LOCUS       DAOQAU010000001       111720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_86_length_111720_cov_7.182859, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000001 DAOQAU010000000
VERSION     DAOQAU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 111720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 111720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111720
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_111720_cov_7.182859"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /locus_tag="PKI94_00005"
     CDS             <1..1895
                     /locus_tag="PKI94_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNW25154.1"
                     /translation="ELKMYENYFDYIICGDVLEHLYNPLDVIICMKHFLQSDGHFIIS
                     LPNIAHGGIKLNLLKNKFNYNDYGLLDKTHIRFFSLKSIIDFMTNASLEIIDINMVVM
                     PIENSIENVQEKEHPIEIVNYIKKDIESYVYQYVIKSKISKLDMQTLFEKNFKKTLLS
                     KEEKQMLMRNLILSEMPHKKLWGSKLRPFLRNLKKKFLPPPVIKTQKIPNTTNIKMQK
                     LVRKFLQISNKYIIKNINRILSKLKNPLFLKTLYINASEKSSEYVNGSKINIKLTDDT
                     MKLIAFYLPQFHPIDENDKWWGKGFTEWINVTKAVPQFLGHYQPHLPDELGFYDLRLN
                     EIMYRQIELAKQYGIYGFCFHYYWFSGKRLLEKPIFDFLNNKELNFPFCLCWANENWT
                     RRWDGQDNEILISQTMEDGDDIKFIEDIIPFFKDSRYIRINNKPVLIIYRIHLYTKEK
                     VQKIVRNWRNYCKQVGIGDLHLIAALTHGFFDNPSEWGLDAAIEFPPHNMNLPNISNY
                     LPIMNPNFKGAIYDLSKYFQQDNYLKKNDFTIYRTVFPNWDNTARLPNNGSIFLNANP
                     ETYKKWLSDAIKFTKTNYSKEDQFVFINAWNEWAEGAHLEPDAKYGYAYLNETKKALE
                     NQFDKN"
     gene            complement(1901..2905)
                     /locus_tag="PKI94_00010"
     CDS             complement(1901..2905)
                     /locus_tag="PKI94_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25155.1"
                     /translation="MKIIFTICSNNYLAKAKALGDSLLRHNSDYKFIICLCDIKHPDI
                     DYSFFEPYRVIEAHNLEIEKFEQMCNKYNIIELNTSIKPFVFDYLFKNYKDVEYIMYF
                     DPDTYVFDNLATIEKELMESSILLTPHIYKPIEFDGKTPVENDFTQHGTYNLGFLALK
                     RSEDVYEILDWWKRRLEVNCYIKPTKGIFVDQLPMNFAPIFFNRVKVSENWGLNVAPW
                     NLHERTLTLKGDKYFINDKYHLIFYHFSDYNFNKADVLSENYTRVSFEDNDVLKRLYE
                     GYKKEILNNKFSELSKIKCVYANDKLRSSSNNIRTIILKIFKYIKKDPLFFLRKTFWL
                     "
     gene            2991..3665
                     /locus_tag="PKI94_00015"
     CDS             2991..3665
                     /locus_tag="PKI94_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HNW25156.1"
                     /translation="MILNFIDILKRKHANKKQRQIIENFHTLYYNNSERTWRNTNWLG
                     INVLKCPLDLWIYQEILFEVKPDLIIETGTYVGGSAYFLASIMDLLENGEIVTIDSEI
                     RGNLPKHKRINYLNGLSTSIEIIEKVKTFTKNKKRILVILDSDHSKENVLKEMRIYNK
                     FVSKDSYLIVEDSNINGHPTLPEFGPGPWEAINSFLNENKEFKIDKEKEKFYFTLNPN
                     GYLKKQ"
     gene            complement(3749..3820)
                     /locus_tag="PKI94_00020"
     tRNA            complement(3749..3820)
                     /locus_tag="PKI94_00020"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3786..3788),aa:Asn,seq:gtt)
     gene            4075..4470
                     /locus_tag="PKI94_00025"
     CDS             4075..4470
                     /locus_tag="PKI94_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25157.1"
                     /translation="MEIQSINSAESGFANYDNLEPNDKASIQKSLQNLGIPLIIINSS
                     ARAIKTYATEHGINLKSISARIDAMIKEQQAKTPAQTPQKDFEDALFLSGIPQEIINK
                     GQNSIERYAKEKGIELPKSPDGTAFNKSF"
     gene            complement(4546..6342)
                     /locus_tag="PKI94_00030"
     CDS             complement(4546..6342)
                     /locus_tag="PKI94_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW25158.1"
                     /translation="MFIGNLRKFIGYFGKGRKIRLLGFFILSLIAGLFEFIGIALVYP
                     FVLLIINPQSVINSSYYIKFSEIFQIKDALVGSFIIGLFVVLLFILKNIFMICVVYLQ
                     NKFANNWKFDISRKFMEYYLFAPYKMALKTQPAQKLYTLNFLIPQSLDGFVFRAVNFA
                     TNFIIVAMILLLLFIKFPFAALVTTVFVGLSLFIQSAFFKNKMKKLSKKLLEVSLESN
                     SQVLNNINNLKELKILSSENYFYEQYLKTQKEFNKAVFQNNFFASIPPYIVEILVVVS
                     LLILAAIISVQTAQNSSVMIASYAITVAAIFRIAPALNKIQTSLNAMNASREVVRGLI
                     TEYEHCDFSSVEKKSDLKIDFKDKIELKNIDFSYSENRKVIKNLSLEIKKGEFIGIIG
                     LSGVGKSTLADIIMGLLPVDAGEIKVDGITLNSENFVALRKIIGYVPQQINILDGTFK
                     QNVAWGVNESDIDDKRVLEVLERAKLIDLVGEFEEGIDSKITSGLRGMSQGQKQRLAI
                     ARAIYRNPQVLIFDEATSALDVETEHEITKMLNDLKGEKTIIAIAHRLSTLKSCDRLV
                     YLKNGEVVATGSFSELSKKFPDFENLVKLSNI"
     gene            6502..7230
                     /locus_tag="PKI94_00035"
     CDS             6502..7230
                     /locus_tag="PKI94_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNW25159.1"
                     /translation="MTNPVFNQNAIDRERVLDSEPMTVNGAINKTFILFCLLLLSSLV
                     VWDMFFKGYTDKVALLTMVGFISSIIAFIVIMFNRNALPIAAPVYAASEGLLLGGISA
                     YIDKAYPGIAIQAVGLTLMALFSMLALYRVGAIRCTDKFRSVLFISTLSIAGIYLVNF
                     IGSFFGMHIPQIFTSSNFGIGFSIVVVAIASLNLIIDFDFIEQGAQNLLSKNFEWYGA
                     FGLMVTLVWLYIEILNLLAKLRDR"
     gene            complement(7770..10268)
                     /gene="leuS"
                     /locus_tag="PKI94_00040"
     CDS             complement(7770..10268)
                     /gene="leuS"
                     /locus_tag="PKI94_00040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNW25160.1"
                     /translation="MKEHYFPQEIEKKLQKKWGECKCFATPTDSDKPKYYALSMFPYP
                     SGRLHMGHVRNYTITDVIARFKKAQGFNVLHPIGWDSFGLPAENAAMQKGANPAQWTD
                     ENIEYMTKQLKMLGLSYDWDREVTTCKPDYYKWTQWLFLQFYKRGLAYKKEAAVNWCE
                     KCATVLANEQVIDGKCWRCDEVVTKKNLSQWFLKITDYAEALLEDLDKLNGWGENVKT
                     MQSNWIGKSTGAILKFKVAEYDMEIPVYTTRPDTALGITYLVVAPEYKDIEALTLPQY
                     KEEVERYRENARKMTEIERLSTERIKTGVPLGTHCINPFTGEKFPLWTADYALAEYGT
                     GAVMAVPAHDERDFDFAKKYNLPIKEVIANNEILKQVQDDVSFSGLTVAYTESGTMVN
                     CGEYDGISNEEFKKIITQKAADGGFGEFKTQYRLRDWLISRQRYWGAPIPIVYCDKCG
                     IQAVNEGDLPVLLPEDVDFKVTGKSPILTSKTFIDTKCPKCGGDAKRETDTMDTFICS
                     SWYYLRYADPKNSQKCFDKNIVDKWLPVDQYVGGIEHAILHLLYSRFFTKAMRDMGLL
                     SFDEPFKNLLTQGMVLKDGSKMSKSKGNTVDPDEIFENYGADTARLFILSDSPPARDF
                     DWSDAGVEGCYKFLNRVWKLVSTNQDKIIFDYKLSENLSKENDDLVRFTHIMIKGITN
                     DIQNEFQFNTVISKYRELTNAIYDYLGKKKDFSQEDKNVFSFAVLTLMKLMSPVAVHL
                     TEEIWTSLGAKTSIHDEKWCEWDENLAKTSSITLVVQVNGKVRDKVEVAQDLSQEKMK
                     QIALNCEKAKEFIAGNEIVKVIVVPKKLVNIVVK"
     gene            complement(10456..11349)
                     /locus_tag="PKI94_00045"
     CDS             complement(10456..11349)
                     /locus_tag="PKI94_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNW25161.1"
                     /translation="MFCKNSFILKTKKKPAFTLPEVLLTLLIIGLIAAVTIPILLGYF
                     EQQKFLSGLKKAYTQFNQALIGTAHDFTCTGDLICTGLFKVGTTSQTLGTELVKHFNV
                     LKNCETNTLGCFSESVSDSYDGSLARTDDYAKISSGFYRFISADGIAFILKNNHNNCS
                     SSMSTGVTQNMEQVCGELYIDLNGLKPPNNMGKDIFRFYITNGKGPLLYPSGGIDDQE
                     NGHWKNGDTAQHCFPSDTDGNYCAARIIEEGWKMDYLANISSLAAAPLSEDPPEEEEE
                     EDPVTPPPDPPSPPGGCIACQ"
     gene            11483..11941
                     /locus_tag="PKI94_00050"
     CDS             11483..11941
                     /locus_tag="PKI94_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNW25162.1"
                     /translation="MSIKPWIEYFLDLAKTCASRSNCIRAQVGAVIVGDDKKIKATGY
                     NGTPSKVQSCYERGECYRIKNNIPSGTRYETCRSIHAEQNAIIQAGQDRCMDATMYIW
                     GHDFICILCKRFIVQSGIKEVYLRKDENSPILKISVEDIRKELEAEPQLV"
     gene            12304..13914
                     /locus_tag="PKI94_00055"
     CDS             12304..13914
                     /locus_tag="PKI94_00055"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNW25163.1"
                     /translation="MTDVQFHSDLDRLIEILPKKITKHITYENMDDVIEIVLDISRLP
                     EVRHSGGRIESLGVEPITDEDINYITQRVQEFTSDNRSGIPGTLHRISAIRNRQGKII
                     GLTCRIGRVVTGTISCIKDFVTQGKSILFLGRPGVGKTTKLREISRLMADELGKRVIV
                     VDTSNEIAGDGDYPHPAIGKARRMQVRQPEYQKDIMIEAVENHTPEVIVVDEIGTEAE
                     AQAARTIAERGVMLIATAHGNSLENLIKNPTLSDLVGSIQSVTLGDDEAKRRGSQKTV
                     LEREKQPTFDIVIEIIDRNTLAVYKDAAEAVDYILRGWPIRPEIRKVDQNYSEKTTET
                     VSNKISQLEQKALPHAEDSLKFSFSRQNYVKEVKNFKKIYIYAVSRSIVEKVIERLDL
                     NAEITRNVEDADIVLAHKNFAKGGAKVLSTANDYRIQVFYVKTNSMAQIQKVIKEALG
                     IQQDTSETLQGYYDEAERALDEAKAAIAKILEGEEHIELQPQNQQIRKLQHELVEQHN
                     LASQSVGEGEQRHLKIVGGKDFKEKNAS"
     gene            complement(14189..15070)
                     /locus_tag="PKI94_00060"
     CDS             complement(14189..15070)
                     /locus_tag="PKI94_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNW25164.1"
                     /translation="MAVTINTNVSSLVAQNNLSKNTEAYQKAMEQISSGLRINRASDD
                     AAGLSISESIKTQLNGNSKAISNIANGQNLLNVAEGGEITVTNHLQRIRELCVQAANE
                     TYTASDKNNILQEIKQRLKDINNISESTTFNGVKLLDGSKTSLSLQIGANSDEATNAL
                     DIGPALADVHISVLGNPVGNVDDPKGIIIPDSTTGETWSTSDIRDYMDRLDTAITSLS
                     SKRALLGAYGNRLAATSSNLTVMNQNLSETNSRILDADIASTSSDLVKYQILQQTSAS
                     ILTQANQLPSIALKLLQ"
     gene            15250..15984
                     /locus_tag="PKI94_00065"
     CDS             15250..15984
                     /locus_tag="PKI94_00065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNW25165.1"
                     /translation="MPHFFISSDSITEGILTISDENNYKHIAKSLRAKIGEKLLFIDE
                     KQIQHEGIVQKITSKLIEVKITKSAKSNRKLDFALYLAQSPLRSDAQSLIVEKATELG
                     VAGLYPILTDNCALNKEVINKKIPKWQKTMAEASKQCERADVPTCFEHATIQTLFEKG
                     SFDRIFAFVERKACYNLKEYLRKNPIKKSEKILVIIGPEGGFSQDELEFFKQNKIPML
                     SLGNLILKAETAAFVALGNIIYEYSN"
     gene            15968..17383
                     /locus_tag="PKI94_00070"
     CDS             15968..17383
                     /locus_tag="PKI94_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW25166.1"
                     /translation="MNTVIEKIKKDKIIKKIKTHCKAAQVYLVGGTIRDFYLGLENFD
                     KDIVIDKINAKDFAKNLAKSLDATFVPLDEENKIYRLVLKDKINYIDIANIIGNSIED
                     DLKRRDLTINSVAINLKTFEIIDITGGVKDLENKKIKHISEQNFVDDPLRLLRAYRFG
                     AVLGFKIDDGLQKIITRHAAKIQKPAVERINYELLKLFEGRFSSQILFEMDKSGLLEE
                     ILPIIKDLKKVPPNSHHHLNLFDHSIETVKQIQRIYEESSNEVKLHLEKHDFGGASRL
                     AHLKFAGFLHDIGKFETWTIEEETGRHRFIKHDDLGSKMAIEFLKKNKFSKKQIDYIS
                     KMIKLHIYPSHVIKNPEINEKLYMRLIRKMKENVIDIIILAMADRYSARGVEITDEIV
                     NKNISDLNLLLEFYLKIKDELKPLPKLLSGDEIMEILNIKPSKELGEIIKKLHESQLS
                     GEIQTKEEALNFIQNFTAKNT"
     gene            complement(17345..18388)
                     /locus_tag="PKI94_00075"
     CDS             complement(17345..18388)
                     /locus_tag="PKI94_00075"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF002727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNW25167.1"
                     /translation="MKRIIDANLNRAAEASRILEEIARFLLDDFDLSEKLKNIRHKIC
                     AFQQEDYDKCLKSRDTQNDVGVDISNPTKRTDVENIFKANIKRLQQALRTLAEYSGQS
                     SDNVKVFEKLRYTSYTLEKIMWEKLKENFSSIKLSDKRLYLVTNSDKFETQDEFLDAV
                     ASALEGGVEIVQLREKTMSAKNLIELGKKVKQLCAQYGAIFIVNDRVDIAMILEADGV
                     HLGQDDLDVKSARALIGEGMIIGVSTHAPEQALKAVEEGADYIGVGPVFETPTKQGRK
                     AVGLDYVKWAAENIEIPFFAIGGIDGANVDKVLGVGAKRIAVVRAIINAKSPQKSAEE
                     FLSVLGGKILNKI"
     gene            18521..19108
                     /locus_tag="PKI94_00080"
     CDS             18521..19108
                     /locus_tag="PKI94_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW25168.1"
                     /translation="MLGKLPYKKIPMEELVMLSQKSDYRALEELIKREQKNIFAAFSY
                     LSKKRENIADLTQEALLRMARGISNLKNPKTFKSWLNQIVTNLFYDDLRKSTRRPETI
                     SIDNDENENPYHTQIPDTKHRPMEKIMSSELDRLIKEEIQNLPDHFRIAIILRELQGL
                     TYEEIANATNTNVGTVKSRIARAREKLQDGLKHYI"
     gene            19120..19671
                     /locus_tag="PKI94_00085"
     CDS             19120..19671
                     /locus_tag="PKI94_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNW25169.1"
                     /translation="MTINLTCTQVSALLSFYLDDRLNQQLKQFVKAHLDICPTCRAKF
                     ETLKKMVESLKEVHEKVATVESQEKEASESPQYNEFRTNLSAYIDNELSDEENIKIKK
                     YVISNPKARLEIENLYEFEKTLHNSFERIKNHSKTDFSKQIMRRIDIQEEINTPDSFA
                     KIIAIFIILFAVCTLGMLFIYWI"
     gene            complement(19672..20736)
                     /locus_tag="PKI94_00090"
     CDS             complement(19672..20736)
                     /locus_tag="PKI94_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW25170.1"
                     /translation="MKLVSTEEIINYKILPFNIYSEFGEKLFSAGEVLTPGKLLQLRH
                     MTVLYRDDEIQAVPAEARVVEAKPVEKEVKPEKPKVVVEVFDMNPSSKITKTKLSVDD
                     IDIMSYKGPVNKRAKIDPQTQIKIKAFYNNILEMMEEKPPLEMLGMFANVRDKIIQDI
                     IFENNDVFLFSQLKLLGEYKKCHALNTAILSGAVAKKMGLNEDLINDVVLAGLLHDIG
                     KTKIPQDLLLKSNLSSYEHKAIQNHTRIGYKIIKHEMDLPENIARVALEHHENNDGSG
                     YPIGKSGDSINVEAQIVNVCNCFDNITSNRTEYNIKNTKEALRIMLQLGTRKFSAEAL
                     YTFIHMFSYNDTVNFEEMLL"
     gene            complement(21366..21680)
                     /locus_tag="PKI94_00095"
     CDS             complement(21366..21680)
                     /locus_tag="PKI94_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25171.1"
                     /translation="MKSREKIVKQMQEVVEQMRLDDIQENPDIVNDFFDCDCCGKFKC
                     LAGSIQYGDYRLCNDCVLYAELGFELKTIKTVDDLIEAMEDKKLEEICEFIRQDTLAQ
                     NN"
     gene            complement(21681..22391)
                     /locus_tag="PKI94_00100"
     CDS             complement(21681..22391)
                     /locus_tag="PKI94_00100"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNW25172.1"
                     /translation="MLLGINIDHIATLRNARGGFEPDLIECALICEKCGVDGITVHLR
                     EDRRHIKDADVRALKKVLKTRMNLEMAMTDEMQKIALELKPSACCIVPEKRQEVTTEG
                     GLDVFSQLERAKKFVKPLNDAGIAVSLFINPDEKQVQAAFETGAEFIELHTGAYSEAF
                     ERENEEEEFLKLKNSASLAQSLGLKVNAGHGLNYKNVHRMKEIPNLVELNIGHSIISR
                     AIYVGLEKAVKEMKELVE"
     gene            complement(22391..23320)
                     /locus_tag="PKI94_00105"
     CDS             complement(22391..23320)
                     /locus_tag="PKI94_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25173.1"
                     /translation="MSAVEEIEELQEILKSDSSNYQARRRLAVLLLDNGFNEEALKQF
                     LFLSGVFPTDAGLFYNLGIVFEKLKKLDNAIHYYKKATEISPDETDFYYNLGLVYIDK
                     QEYDKAIECFKKVLTKDKDDSNSYFNLGLCFFKQGKYEPAAKCFNKALEYNPCDVFAY
                     FYLGYIHKNKGETEEAKEKYYKVIEVCPEYSWAYFNIASIAFEEGEMELAVENLQKTL
                     ELNPLDIEAYKIYSKILIKSGEIQEAQKLIEHAITENPDKADLYYVLAQIYKLQGNNE
                     DYVKALKHALKQYKTLSFSPKAVKQELDKIEVK"
     gene            23665..24438
                     /locus_tag="PKI94_00110"
     CDS             23665..24438
                     /locus_tag="PKI94_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW25174.1"
                     /translation="MIKFTKNTKVSTMSQGYTGFTLAEVLITLTIIGVVAALTVPNLY
                     AYYQKTQYVTQLKKVYSEISQAIKLLMADEGVNKLSASNTLTFDEVSDYDEFIQGLGD
                     NFFKKYFKVIKDCGTGYECFANNYKTIDENNFSGITCSYSVIIASGASICTSQIASPS
                     MPGDFFVDINGLNPPNVAGRDLFSFSYYHDGSIDSFVSPECRKGVPNSSGLLCFGHNS
                     PKEARDYFFDNGEGTGCLKSTYGVGCFAKILNDNWKMDY"
     gene            24817..26370
                     /locus_tag="PKI94_00115"
     CDS             24817..26370
                     /locus_tag="PKI94_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25175.1"
                     /translation="MEKVKPIVFYLIFFLFILAYCTGAGGYDYDLWARLIAGMGFVQG
                     GHVLKQDFLSYTPTHTWFDHEWGSGVIFYLVQHFFPTAGLIVLNAVLIFILFFIITKI
                     VNLRGVKVTTAYNFIFYLFAFLAIAINVMEPIRCQMFSFIFFALFIYILELARKGQNR
                     PLWLLPFIMIIWNNLHGGCVSGIGLICLYILGEFLNKKMFKKYIFTLIPTILVLVINP
                     WGFSYLNFLIDAVTMKRPDIMEWWGLFHKIHIHNFLIFKLFSLTMILTYLISSVKNFS
                     YKNIDKTKFLILSVTLYQAIIHIKMIPFFVISATCFLYDDFYTLLDNIIKFIKQKLKI
                     PAEFFGEKFIVKKEFAIYCFIIICSIATIHTNNLGIILKSDKFAYKEIEFLKINNIKG
                     NLFLNFGQGSYAAYKLYPNIKIFMDGRYEEVYYNYMLPVMNEFYYSKVDNTKLMKKFP
                     PDIMIIEKNYPVYKNLLKHKNWKLAYEGIFYGVFLNKKDLKKNYKLPSNDRKYYKKTL
                     FDTNINFKK"
     gene            26398..27600
                     /locus_tag="PKI94_00120"
     CDS             26398..27600
                     /locus_tag="PKI94_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNW25176.1"
                     /translation="MSRENNSLKYTCIFGGGAIRGVSYVGTIRALEEIGICADTIAGS
                     SVGAIFAGLWATGYKADELKDIFMHTNYELFRDIHFGFGKDFAISKGEIFLDWLRDLI
                     EQKFYGERYKKGENPPVTFKDLDKNVVIITTDLTNFKYKEFSKFETPDVEIAYAIRIS
                     STMPGLMKPIVYQDSLLVDGDLQKSWPLWRLSKNLCPDDQRVLEFRLEGDYEGKGSNA
                     INFINTIYSCVTSLATDFIIDTYGNKDKFDYIKVNTGAVVVVDFNQPKEKREELMDIG
                     YKQTVDYFEVDLRKKKREILKQYERILKHVKKIKNDVESNKIKQAQSGLGLLYMDLSS
                     IKKVIDLKYFNLIDDFKECFMSNVNPASLFGIFSLNNSKLVKSKINAINFELEEKVAE
                     LESYVFKI"
     gene            27610..28635
                     /gene="ilvC"
                     /locus_tag="PKI94_00125"
     CDS             27610..28635
                     /gene="ilvC"
                     /locus_tag="PKI94_00125"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNW25177.1"
                     /translation="MQELKIYKDKDIDEKIIKDKKITIIGYGSQGYGQSQNLRDFGCN
                     VILGLRKDGVSYKKALAEKFEIMEVEDAVKWADIIQILVPDEIQAKLYKEKIAPYLRT
                     GQYLMFSHGFNVHFGKIVAPKDVNVIMVAPKSPGHALRSEFLKEKGVPALVAVYQDYS
                     GDSKEMALAYAKAIGSGRAGIIETTFREETETDLFGEQAVLCGGCSALIKAGFETLVE
                     AGYSPYMAYFEVLHELKLIVDLINQGGLTDMHYSISNTAEYGDLTRGPRVINDNTKTE
                     MKKILKEIQNGTFAKEFIDEINSGSKNFDKLRKQIEEHQIEKVGKTIRTNFSWGSENK
                     IIDREKN"
     gene            28644..29474
                     /locus_tag="PKI94_00130"
     CDS             28644..29474
                     /locus_tag="PKI94_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW25178.1"
                     /translation="MFDNETIYEVVIFSIGDTKSGTKMGKLQLKNTQNETLLNCILWE
                     ETLNRIDSKLFRTGNLVKIVSATYNEKFNNCLVSALELVKEAKLGIDESERNSLYSEI
                     LEYVNKISDEKLKNFIIDYFKEHEKEIKIAPAAKLMHHNYMGGLLVHIVECLKFADAN
                     MELFKIKIDKNKIYAACILHDIGKIFEYKIDTETGIIDYDENFRHDWVTHSQYGFSIC
                     MAQGFKDVARMIAAHHARSDWGAIIDLDQKDLESWVYLMHHIDDLSAKYGRISVSLLS
                     "
     gene            29549..29851
                     /locus_tag="PKI94_00135"
     CDS             29549..29851
                     /locus_tag="PKI94_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25179.1"
                     /translation="MHKRWYDADPTVSLAISLLRNTNEKTQLRCADFIIDYAKQRNVT
                     RPANALNDAFNYILRRWYDQDKKIAEAFEFFEAAQPDVQKEISIEIINFLQFSELY"
     gene            complement(29848..30810)
                     /locus_tag="PKI94_00140"
     CDS             complement(29848..30810)
                     /locus_tag="PKI94_00140"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNW25180.1"
                     /translation="MPKVVILGYGEMFMNLIAGCLDAHCDVVGVFRYDKVRHKGLDGL
                     ILDIFNPSKEFNYIKGHKLYEIKAKSANSVEFKKEILKLNPDIVFVATWGEKLKKSII
                     DLPKIATINVHPSLLPKYRGPNPYLQAIKHLEQQSGVTFHLMDKDFDTGPILLQKTVE
                     ITDKDTGKELKEKIAGATRQAVTELIKELGEEIIIPVVQDEKKASYFSVITEDEVMLD
                     FSKSAEELSAQIRAFHPWHKCYYEYKNQFFVPNPYCLEILENNTNFNEVGSIVAKSPK
                     NNQITVLCGDGKLLKMSRVRLYGKIRKFFTNFYIKYLIKKGEKA"
     gene            complement(30877..31947)
                     /locus_tag="PKI94_00145"
     CDS             complement(30877..31947)
                     /locus_tag="PKI94_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNW25181.1"
                     /translation="MADLKQQKFDTSMEAEIFEQPEILRQILEKYIAPNGYIILNIPT
                     KITKIVLVASGTSYHCARLAAEMFGEIADIEARAIYSSEFLLKKIVPHDKNILYIFIT
                     QSGETTDTLSALNKAKQLGVKTMCITNKENSTAWQTADYRINCYAGEEKSIASTKSLT
                     AQMLCLYLMALKYAQIHEVDITEQLEKVKTLPEILEKTLELSPQIKALARFLSKYSNI
                     LITADGISYALAKEACLKIKETSYINVMSHILGEFMHGHVAVLNNKCVLVYVSNKPIS
                     YSTIKHMEKIKRDYNPPICVIGTSSDRINSAFNVDLEVDDENLKIFSMAVMVQLLALK
                     IAQKLGRNVDKPKGLQKVVTEV"
     gene            complement(32066..33514)
                     /gene="gatB"
                     /locus_tag="PKI94_00150"
     CDS             complement(32066..33514)
                     /gene="gatB"
                     /locus_tag="PKI94_00150"
                     /inference="COORDINATES: protein motif:HMM:NF004015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNW25182.1"
                     /translation="MEQELKDKYEVVIGLEVHAQLKTKSKIFAPDGTEFGNEQNTQIS
                     PITLGMPGVLPVLNKEVVNMAILTGLALNCQIPNRCKFDRKQYFYPDLPKGYQISQYD
                     EPICVNGSVEIDGKRIGITRAHLEEDAGKLVHAGADGIHGSSYSLVDLNRAGTPLLEI
                     VSEPDMRSSDEAKAYVEHLRNILRYIGVCDGNLEEGSMRCDANISVMPKDSKEFGTRA
                     EIKNVNSFRALQRAIEFEIERQIDIVESGEKVIQETRLWNDNTGETKSMRGKEDAHDY
                     RYFPEPDLMPLEISKEWVEEIRAKMPELPSAKIERYMGLGLSEYDAKVIVEEMETALF
                     FDEVIKLGANVKTAVNFLMGDIAALLKEEKININQTKLTPQNLAELVNLIEKGTISNN
                     IGKQIIPQMLKEGTKASEIIEKQGLSQISDEGAIKEIVKKIIEANPEQVSAYKAGKVQ
                     LLGFFVGQVMKETQGRANPKTVNELIKSLLES"
     gene            33598..34419
                     /gene="kdsA"
                     /locus_tag="PKI94_00155"
     CDS             33598..34419
                     /gene="kdsA"
                     /locus_tag="PKI94_00155"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNW25183.1"
                     /translation="MTEVKKIKLKNFEIGGNKLTIIAGPCVIESMDILNKTAEGLKKI
                     TEVLDVNFIFKSSFDKANRSSINSFRGPGIEKGLEMLSKIKKEFDLPIVTDVHTESET
                     KIAAEVADIIQIPAFLCRQTDLLIAAAKTGKIVNIKKGQFLAPEQMKSLIKKVTDSSN
                     ADVLLTERGTTFGYNNLVSDFRSIPIMQEFGYPVIFDATHSVQLPGVGEECSGGDRRF
                     VPVLAKAAVAAGADGLFFEVHPDPDKALCDGPNMIALKDAQKLFKTCRDIFEIVK"
     gene            34419..35036
                     /locus_tag="PKI94_00160"
     CDS             34419..35036
                     /locus_tag="PKI94_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW25184.1"
                     /translation="MILKHFTVGFLEVNNYLLIDEKSKEAILIDCTDKNPQIEEAIRE
                     LGAKLKYILLTHGHFDHVLGVNEFKKLYGCKVLIHKNDEIMLENVDKISSSFGFGKSE
                     AQHTDGYVEDNQSIYFGGHEIKVIHTPGHSQGSVCYLIDDMLFSGDTLFKECVGRTDL
                     YGGNFDQLKANIENKLFTLDGHTKVFPGHGEFSTIEYERVNNEFL"
     gene            35150..36184
                     /gene="pheS"
                     /locus_tag="PKI94_00165"
     CDS             35150..36184
                     /gene="pheS"
                     /locus_tag="PKI94_00165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNW25185.1"
                     /translation="MKETLETILKNAKSDIQKASTTEDIEEIKNKYLSRKGELNSIKK
                     NLKDLSDDDKRIIGALANDIAQELESALDEKFNTIYRKELEQQLKKEQIDITLPGNFT
                     PKGNVHPLTSTTNEIVSILQTLGFSLVQSENSPEVETEYFNFDMLNFPPDHPARDMQD
                     TFYTKIASNVILRSQTSNAQVREMTVKKPPLRVISPGRVYRSEAVNARKNNLFHQIEG
                     LYVDKDITFADLKGVLNEFIRLYFGSSRVTRFRSSFFPFTEPSAEIDVQCIICGGKGC
                     KTCSNTGWLEILGAGMVDVNVLKNVGIDPEIYTGFAFGMGVERLAMLKYAIDDIRLFF
                     NNDERFLKQF"
     assembly_gap    36238..36337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            36406..37113
                     /locus_tag="PKI94_00170"
     CDS             36406..37113
                     /locus_tag="PKI94_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25186.1"
                     /translation="MASIIDSFRETFTDNMSFFKLLTFAVPLYFAHDLYLKNPQNPTG
                     ALWLAGITIFFLFGFLIKVTGEVLNEGDTVLPFLNPLKLALASLKGIIAIGPITWLSA
                     SIATYVIPKVNVIGWFNITFQIVIWLIVIGIALTSFLMFVKNEKIADSYNLKVLFNKS
                     VDLSIAIIFFIFQLTLINIPTNVFIGYIILVLFGFGPLFYFFAAYAIVFNVAVTGHYM
                     AQLQYEVLGYDKNKMDF"
     gene            complement(37118..39103)
                     /gene="uvrB"
                     /locus_tag="PKI94_00175"
     CDS             complement(37118..39103)
                     /gene="uvrB"
                     /locus_tag="PKI94_00175"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNW25187.1"
                     /translation="MENKFKIHSKYKPSGDQPQAIEKLTKGLNCGDDSQTLLGITGSG
                     KTFTIANVIEQVQKPTLVIAHNKTLAAQLYNEFKELFPDNAVEYFISYYDYYQPEAYI
                     PRTDTYIEKSSSINEEIDRLRHNTTRSLYERNDVIIVASVSCIYGLGLPENYFKGSIE
                     LSVGKEISRQDFLRHLVEVRYIRNDLVLERSTFRARGDIVEIMPAYEKIVTRIYFFGD
                     EIEKIVKIDSVTGEILETPQKTVIYPAVHYLSYDENVDETIGLIKEELKARLATLNEQ
                     NKLIEAQRLEQRVNYDIEMIKEMGYCSGIENYSRIIERRPVGSAPSTLLDYFRGDFLT
                     VIDESHVTLPQLKGMYRGDMARKETLVEYGFRLPCAKDNRPLKENEFFDKVHQKIYVS
                     ATPAEFEKQTSAQLVEQIIRPTGLVDPKISVRPIETQIDDLIKEIDLRAQKNERVLIT
                     TLTKKMAEDLCDYFLQQGVRVRYLHSEIQSLERVEILRDLRLGEFDVLIGVNLLREGL
                     DIPEVSLVAIMDADKEGFLRSQTSLIQTIGRAARNSCGAVIMYADKITDSMKKAIDET
                     NRRREIQLNYNKKYGIIPHTIKKPIENNLLSLVASYRNLEDIVAEEMVDLGISEKELP
                     KLIDKLEKDMHKAAKILDFERAAQIRDQLKKLREMVK"
     gene            complement(39121..39621)
                     /locus_tag="PKI94_00180"
     CDS             complement(39121..39621)
                     /locus_tag="PKI94_00180"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNW25188.1"
                     /translation="MAKECSFDVVSEFDRQELVNAVDQVEREIISRFDLKNSNSKVEL
                     EADKAITITTCDDMKLRNILDILQSKLVKRNLSIKILDPQPMENALGGNVRQVYKLKK
                     GLTQELAKKIVSDIKDSKIKVQASIQGDQVRVSGKDKDDLQAVIKILRENEEKYDYPL
                     QFQNYR"
     gene            complement(39718..40605)
                     /locus_tag="PKI94_00185"
     CDS             complement(39718..40605)
                     /locus_tag="PKI94_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25189.1"
                     /translation="MNYLPDEYGNTYGINPGTTGFNPYLMNTMTMPGMTVPGYGVNGG
                     LNTNYLGRPAPLDAFVHREKDDTALKAGTIIGTIALLIGSLALLRKGKGTKAVKEAAK
                     AAQEAIKPATEAVANVVDDVAKPATEAVEQVADTVAKKSKYTLARGKYKIKTPQTIKK
                     GIQEAANVAEEASKSAIEVIKNTSDDVATFYNRPKSPSKVKTNHYWEYTAEQNKLRKE
                     ASETVFIGEGSNSVRATKRIIETPYAVNVPKEPVITTPYAVNIPKEPLIESAYIHKPT
                     EPIIESAYVQKPKGEIILP"
     gene            40867..41682
                     /locus_tag="PKI94_00190"
     CDS             40867..41682
                     /locus_tag="PKI94_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNW25190.1"
                     /translation="MSLMKGKKGVIFGVSNQRGIAYAIAEQLHNAGAEIAFTYAGEIM
                     ESRVRPLAEGMNAKIIMECDVTKEEHVKKVFEQCEKVYGKIDFVVHAVAFANKEDLMG
                     KFIDTTKEGWDLALGVSAYSLISIARNAQPIMNEGGSIFALTYLGSDYVVANYNVMGV
                     AKAALESSMRYLAEDLGKYGIRVNAISAGAVKTLAAKGIAGFDKMLKAAVVKAPLRRN
                     VSLEDVGKAGLYLASDLSSGTTGQILFVDCGCHCVYASGEEMELISNCIQAQS"
     gene            complement(41739..42047)
                     /locus_tag="PKI94_00195"
     CDS             complement(41739..42047)
                     /locus_tag="PKI94_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25191.1"
                     /translation="MILSEFAKYLQEREDEIIEKQSCTKLLGFWLKEILERKPKTNVE
                     KIIHAEISLAKNKAGDFLLVAKSPSGQKLTNALYNFALSFEHHVMKKWLKDKKPDDFK
                     "
     gene            complement(42044..42709)
                     /locus_tag="PKI94_00200"
     CDS             complement(42044..42709)
                     /locus_tag="PKI94_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25192.1"
                     /translation="MKKFISYCLDWIYKKKCYFCSSSQESVKMCSKCFSNLEHLPEFE
                     QREICGVKVFCAGVYEGELQKLIRGIKYHKQRDLAYYQAKFMHTFWLKTGDLDKCYQI
                     VPVPMFKAREKQRKYNHMVLVAQEFSKLTGYEVNLDLVQRIKDTKPQYNLKKDQRMQN
                     LSGAFKVNKNKLLKGDILLMDDICSTGATFENIILELKNQGIENIVCLSASTPFAQSG
                     VCV"
     gene            42764..44131
                     /gene="rseP"
                     /locus_tag="PKI94_00205"
     CDS             42764..44131
                     /gene="rseP"
                     /locus_tag="PKI94_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNW25193.1"
                     /translation="MSIIIMLLLLSVLILVHEAGHFIAARYFKIKVDKFGFGLPIGPT
                     LYETKWGDTKILIHAFLLGGYVAFPDDEKDCELSKDSPERFINKPVYQRAIVVSAGVF
                     ANILCAFFFVFLTAALWGNLPSGKYEIYVNDIVAPKQESIWQSGLQKKDRIIEINGTK
                     VENTYALLDFIKMSKSYDGKIDANTKTDNYLRLKALNSVFTQDEIIPEGIAVKLPEKT
                     FEAPVKIEQKVLKGMKKHVDKQISLNSKQTKLRDKLIGQTAYISDGTCTLDDLAYAIS
                     DNSRPLDIKVLRNGKTINLKTVYANKKGLLGIKLEAKEVLIPTKTPQQVITKGTKYLW
                     DNTYMLLYGLYQIFSGQIPLKDLHGIVAITKVGGDIITNSGIFFGLLLIAIISMDLAI
                     INFLPIPALDGGHIMFLIIEKLRGKPLDEKVIDKIGSVGFAFLITLMVFVIFNDIAGL
                     ITKKF"
     gene            44222..44866
                     /locus_tag="PKI94_00210"
     CDS             44222..44866
                     /locus_tag="PKI94_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW25194.1"
                     /translation="MVDKITIELDEQFLKKFFGLTEKFIPEDQEITLLMLRKQLEEIE
                     EAIFNAKMTSSAAAAAPAYQKPSGPQAPTNVDAFGEEIHNGDEFNVPKKSILIIDDLG
                     VITYQLSVLFRNMGYYVVDSKEIYDAIEKFKKNNFDLVIMDLFIPTEREGFILLEELK
                     KTSLSKQKQARIGIMSASAKKEHKQICKMKGAEFYVEKVDDWQKELINVIQNLS"
     gene            complement(44863..45792)
                     /gene="atpG"
                     /locus_tag="PKI94_00215"
     CDS             complement(44863..45792)
                     /gene="atpG"
                     /locus_tag="PKI94_00215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNW25195.1"
                     /translation="MANLKDIKSRILSVQNTKKITRAMKMVAAANVKKAENAVKASRP
                     FSEELIIAFKKLLSAVGDYSSEGLKIKLAIDNYPELLKKHEVKTAGLLVITSNKGLAG
                     AYNANVVRNTIKTIEEYKQQGINCKLFIIGQKGNAALKRKAEDYGFEIVKTYIKVAGE
                     ISAAGAQYVAEDLSQAYVSGEIDTIEIFTTKFKNMMSYLAEKWNLLPINAEEDLKTES
                     AVKIDPVMIFEPNEHSILQKVVPMYITNTIFQSLLEAQASELASRMTAMSAASNNAEE
                     MIRTLTIDYNKARQAAITQELLEVVSGADALKG"
     gene            complement(45904..47409)
                     /gene="atpA"
                     /locus_tag="PKI94_00220"
     CDS             complement(45904..47409)
                     /gene="atpA"
                     /locus_tag="PKI94_00220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNW25196.1"
                     /translation="MAVIKPDEISSIIKSKIENYESQTEISNVGSVLEVGDGIARLYG
                     LRNVMANELIEFQDGKGTLGITLNLEEDNVGVVILGEYQHIKEGMLVKTTGRITSVPV
                     GDGLIGRIVDPTGQPIDGKGEIKADKTLPVEKVAPGIVKRKSVHQPLQTGLTSIDALT
                     PIGRGQRELIIGDRQTGKTAIAIDTIINQKGQDVICIYVAIGQKASTVAQLAKTLESY
                     GAMEYTIIVAASANESAPLQYIAPFAGCAMAEEFLEQGKHVLIIYDDLTKHAQAYRAM
                     SLLLRRPPGREAYPGDVFYLHSRLLERAVKLSDALGGGSITALPIIETQAGDVSAYIP
                     TNVISITDGQIFLESGLFNAGIRPAINAGISVSRVGGAAQTKGIKQVAGKLRLDLAQF
                     RELEAFAQFASDLDKATKQQLLRGQKLVEVLKQPQYQPLSVAQQVTVLFAANEGLLDE
                     VENREIPTFKKGWFEYFDANMPELSQRLNAGEALSDEDKEALKNAILKFKG"
     gene            complement(47429..47983)
                     /gene="atpH"
                     /locus_tag="PKI94_00225"
     CDS             complement(47429..47983)
                     /gene="atpH"
                     /locus_tag="PKI94_00225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNW25197.1"
                     /translation="MANNQTKNILIAQRYAEALVELVKDGKLTYEKVSADIKTVLQTL
                     KTSFDLQEFLTNPLISIEDKKEVAARVFTDEIDTLVLNFIKILIDKDRFCQFEAVSEA
                     YDKILDKINNVSRIKVTSAVALGEDAKKRLKEKLENKLSKKVTFDWEISPEIIAGLVI
                     QMGDNVIDTSLKHKLEDLSKSITK"
     gene            complement(47973..48494)
                     /locus_tag="PKI94_00230"
     CDS             complement(47973..48494)
                     /locus_tag="PKI94_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNW25198.1"
                     /translation="MINQQIQQIWHIIVESNTFNFIFFAAIFIWIFKKIDIKKAIESL
                     HTKIVEAIEAAKKSKEEAHANLKQVEKLVENLPQELEEMIKEAKASAHVIGEKILDDA
                     KKQVENIGTNAKKIIDAEEKMIISKLTQKASKASIEAAKSHITNSLVSNPELHEKYIN
                     ESIEELDRLNFGK"
     gene            complement(48498..48911)
                     /locus_tag="PKI94_00235"
     CDS             complement(48498..48911)
                     /locus_tag="PKI94_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNW25199.1"
                     /translation="MEFNATFIVSAISFIVFVLIMNGIFYKPLSKIVSQRQEFLDEQY
                     HQANLAKQKSEALIADKENKVEESKTRAKKIIFDTSESAKRQKADLTGIAQKRASEEV
                     SGAKEELEKSKAEAQKVLEDEVKKLAQTISTKILG"
     gene            complement(49027..49278)
                     /gene="atpE"
                     /locus_tag="PKI94_00240"
     CDS             complement(49027..49278)
                     /gene="atpE"
                     /locus_tag="PKI94_00240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNW25200.1"
                     /translation="MAAEEIKTLSEMAQLGAGIAIGFGAVGAGLGIGVATKGFLESVA
                     RQPEIAGKAVGFFLLGAALSEACALYAFVIALKLSGMMG"
     gene            complement(49319..50011)
                     /gene="atpB"
                     /locus_tag="PKI94_00245"
     CDS             complement(49319..50011)
                     /gene="atpB"
                     /locus_tag="PKI94_00245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNW25201.1"
                     /translation="MSEAGHWIAQVGNYSFNMDTLVTMWTAMAFLIIFALIATRKLTL
                     IPSKIQAFSESLMGFFWDLTDSMIGKEGRKHIPLVASLFLFIITANLMGQLPLRLIQL
                     KQGELASPTNDINMTAAMAIIVLVYYVFTGVKKKGPKYFLHGFDFISIILALVEVLDM
                     FTRPLTLALRLFANILAGEILVSALLGMCAYFLPLPIMFFEVLVACIQAMVFMMLTIA
                     YIAMAVEEPEHH"
     gene            50139..50861
                     /locus_tag="PKI94_00250"
     CDS             50139..50861
                     /locus_tag="PKI94_00250"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNW25202.1"
                     /translation="MKLIFFEKIDSTNTYAKLNIDDLEDKSVVYTNFQTRGRGRFDRS
                     WVNLGSENIYMTIILKPSEKFEQNYSNLTQYLSLCLCKQLKEPGLTPQIKWPNDVLVN
                     GKKIAGILAETVIKGGNLKGIALGIGVNLNADLQDVLNIDRPATSLNLELGKNIDKPK
                     FMKKLLEEFFESYDDFLKHGFKFIKEDYEKRACFLGQNLKVVIFDIIKEGYCKGLDDF
                     GNLILTDKNGHEQSINMGEIIE"
     gene            complement(50909..52033)
                     /locus_tag="PKI94_00255"
     CDS             complement(50909..52033)
                     /locus_tag="PKI94_00255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNW25203.1"
                     /translation="MIPILDLKRQYDKIGKEIEKEVVAVLRSGSYILGNHNKELEKEL
                     SEYIGVKHAIALNSGTDALHLALRALDIGAGDEVISVAFTFVATTEAIGIVGAKPVFV
                     DIDPDTFNIDVEKIENAITPKTKAIIPVHLYGQPCNMDVIMDIAKKHNLYVIEDCCQA
                     IGAKYKGKMVGSFGDVGCFSFFPTKNLGAMGDGGLLTTNSQSIKDRVIGLRNHGGAVR
                     YYHDEIGVNSRLDEVQAAILRVKFKHIDEWNKKRRQNAYRYNELFANCADVQTPKELD
                     DTYCVYHQYTIKIPNRDEVHKMLQEAGIGAMLYYPVPLHLQKVHEYLGVKEGSLPATE
                     ADTKVVISLPMFPELTLDEQKTVVNTVVECINKTKNPVGV"
     gene            52666..53490
                     /locus_tag="PKI94_00260"
     CDS             52666..53490
                     /locus_tag="PKI94_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNW25204.1"
                     /translation="MTKLRIFLGYFILIIISLSMLLPFFAMLSLSFMPGSEIFSYPPK
                     LFSKNITVQNYFSVFTSIPVTRYFLNSLFVATITTIGQVFFAALAGYAFARLKFKGKD
                     ILFFIFLITMMIPPQVNIIPLFFLMRELHLVDTYQALIIPGFFGGFGVFMLRQYFLNL
                     PKELEESSKIDGCNIFQMFFKIALPLALPAVATLAIFTFVSSWNSFMWPLIITSSDSM
                     RTLPVGLAAFKGSFREVTMWGDLLACAVICTLPVVGIFLLGKKYFINDLLQGGVKE"
     gene            53628..55079
                     /locus_tag="PKI94_00265"
     CDS             53628..55079
                     /locus_tag="PKI94_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNW25205.1"
                     /translation="MSETNFIYTNQNGVSEALYNNFLFVENTARLKSIKNFEKTKLCV
                     IKTDFGKNDLKTIEKIKSENQDVEFWICSNNSSRKNILAANKIGINTVLACPFDISMI
                     EEYLNSQQFPYLNNLSENNYSGLEGLKVMIVDDNVMNVELLEEVLSELNLRISSFTKP
                     AQAMEFVLREKFDLFLLDIMMPEISGFDLIKIIKKTPLNQNAPIIFISALSDAQNKIK
                     SYNLGSYAYIEKPFDINIVKSQIYNILKNHKIQEILSSDKETFLATVAHDLKTPIKAE
                     INALNMLLDDKFDKMEVDKQEILEDILSSTRFMQDLVENLLCKNKIEQEKINLCKDFY
                     CVKELVLHCIELTKYILLPKKQKINFKCKTEETLLFFDFLEMKRALHNLIANASEYSS
                     EKDEILVEIFKEGNNLGLSVQDFGRGIKLEHQKDVFSQYMSLAKQQKTVGSGLGLYIT
                     KSIVEAHQGEILLDSKVGEGTKITIMLPIYTKV"
     gene            55165..55530
                     /locus_tag="PKI94_00270"
     CDS             55165..55530
                     /locus_tag="PKI94_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25206.1"
                     /translation="MKKQLPYEKNSNCIFKLNSDNISSNLVYKLKKTFKKLPKNKSVA
                     INLQNVEYVCSEFLEFLKEFSKKREISLFNIQAEISALFYLTKYDKFAHIYLNDIDFY
                     EQKRMLLNRNFSILNNFKQ"
     gene            55578..56300
                     /locus_tag="PKI94_00275"
     CDS             55578..56300
                     /locus_tag="PKI94_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25207.1"
                     /translation="MFDFVQKFIYWVLSLQEKRLKHKLGGSLKRSFSNSTSKIVFSKS
                     DSLKLNSMTEKNKERLEKEVKIILKHCENNPDKVFEFIEKKGTKVHKIPFAHKILKLI
                     GLEEGLVAKLNGIKALYLTSVLAVLGKENKISLTTKPMFVMRDLPLNSYYTIQQFHKW
                     YATKLDMPGFDAKSQEHFQKYLDNINDESIKSLSMEEIIGLKEAIARDVEAINFVISL
                     AKSTEGAKNALNKMATEKGASV"
     gene            complement(56327..56935)
                     /locus_tag="PKI94_00280"
     CDS             complement(56327..56935)
                     /locus_tag="PKI94_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW25208.1"
                     /translation="MRKKGFTLAEVLITLAIIGVVAALTIPMVINRTNEKELRVALKK
                     AYSTLNQVSIQVLSEDPDFYKKLGSELTNEDFQSTFEQYLSVIKSCDNSTIAGNCFVE
                     EVKTLDGAGGFYVSDDGKVGSILKDGTAIAYDLANAGFLVDVNGLKPPNVMGKDVFSI
                     IYDCNGNGIYDRLCPYGYWVAPTEIECPSGNGFDCAAKYLNE"
     gene            complement(56956..57684)
                     /locus_tag="PKI94_00285"
     CDS             complement(56956..57684)
                     /locus_tag="PKI94_00285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW25209.1"
                     /translation="MNKNVLITGASRGIGRAIALELKALGYNLFLSAKNETNLTKIAQ
                     ELGVKDFIACDLSKPNEVEKLGNFIQKNEINVLVNNAGEYLYANIENADREKISQIFE
                     VNLLSPIVLCSKAVPFMKVQKWGRIVNIGSISGVMGEAYASLYSGSKSGLIGFTKALA
                     LELAQHNITVNTINPGWVQTDLGEKSIEKSDFSLEETLDMIPQRRFVEPKEVAKLVAY
                     LLSNDARGITGQSINLCAGLSVGV"
     gene            complement(57677..58006)
                     /locus_tag="PKI94_00290"
     CDS             complement(57677..58006)
                     /locus_tag="PKI94_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25210.1"
                     /translation="MKHVRWYDKNPDLKDVFEFIENLDEQRQGDIAKDILQILVNDFN
                     FDLDEKINNISKNYTYKCKRWYDTNIDLHTSFEIIKDVPEDVQCQIVRKIIQTVLLIY
                     FESGGNE"
     gene            complement(58055..58294)
                     /locus_tag="PKI94_00295"
     CDS             complement(58055..58294)
                     /locus_tag="PKI94_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25211.1"
                     /translation="MVANIVNLLSRAFSANNLQKTTALSPVSNSNPFSNPFVTSSTAK
                     SYAGYAKNKPVQGGYFAGYYNGRPNIVGQRLFLEV"
     gene            complement(58490..58804)
                     /locus_tag="PKI94_00300"
     CDS             complement(58490..58804)
                     /locus_tag="PKI94_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25212.1"
                     /translation="MQAIINKIENMNPIHYFLSQLEEADNNVKNQIENTLVNIGAQAV
                     PELVSQLQVVKGVKRGVVAMTLIRIGDASVEYLKKAAADNEDFAWIAKYLISEIKGSI
                     AA"
     gene            59147..59449
                     /locus_tag="PKI94_00305"
     CDS             59147..59449
                     /locus_tag="PKI94_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25213.1"
                     /translation="MENCQADCSRHGHMELKNVNKEIISWLEDVIDENNSRVERKEWK
                     SKYNSYVVYDYEPFCAEGFEINLLITSYDSAYLNFIKYLYDEKINTIQYLNNCIGV"
     gene            complement(59512..59871)
                     /locus_tag="PKI94_00310"
     CDS             complement(59512..59871)
                     /locus_tag="PKI94_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNW25214.1"
                     /translation="MENKRVNSIIEALAKHEDVQSVNVLERVGTNCENDDVRRLTAKA
                     LIKKNNQDSLAVVISSKGKGINDLSTSVAMSTINELLQLKDKEEAISVLENTENNHED
                     DEVRETARSVKALMSFS"
     gene            complement(59884..60351)
                     /locus_tag="PKI94_00315"
     CDS             complement(59884..60351)
                     /locus_tag="PKI94_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25215.1"
                     /translation="MEKLSINNISKNFFRTTTVKKVDKNHTNPFGVSFKGNVLTADVF
                     ETKNNNVNLIERAKNKSRMLSSAVVGSITNLSSAISTRLNSVVSFGNRIRQNATDFWT
                     KAQQSEIHIDMAKLSNKITSKMPSFTGTYKVSNLVKRPVSELEEMLKAELATI"
     gene            complement(60582..61436)
                     /locus_tag="PKI94_00320"
     CDS             complement(60582..61436)
                     /locus_tag="PKI94_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25216.1"
                     /translation="MIALIEKENNTSDKILKPDFNQKTGRELLAFYLQSKFKQILAHD
                     KKYHTPIFIEVRPDFISRFSKRLINNPQKKILVGITGESACGKSTICNKIKDTIALLN
                     LPVTIISSDNYFNDISDLISRWGDFDTLRDNGYDVDAPESFQLDLLKEDLEIISKGLD
                     IKIPQYLLNGTGVSVPKSIPVQAQKIVVVEGMSTMYKDVKEIFDIKIYIEADCQERQR
                     RFMSRAVCRNQDECNAQKHWEYVKIAGEKYIKPFRNEADMILNGNSDLEYFSQILEYI
                     YTITNNFQ"
     gene            complement(61699..62721)
                     /locus_tag="PKI94_00325"
     CDS             complement(61699..62721)
                     /locus_tag="PKI94_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25217.1"
                     /translation="MNIKPLNYSLETNQIENEEEVKTTEESSTENTEGKTAEEAIDNA
                     EYSVIDSEMVEEEDSFDGLKDLMKKCVDAFQEMTNYISQLVSGNSSKAEQAQTLKDEI
                     SSISTEDGTGAGKNSAYSLNLAGTEDSEDNTGQNSDNTEASSKLEEKSSALDSLGEDI
                     QTNADKIGKVSKDIDTTDSAVKDILAKVKKGKSGKKSLLAKIGLGSLAVGGIGAAAVV
                     GVKSLIAVQLAGTAVVDATTGAVVGVVGAAAFSPALLAVAVIGVGVAAAALFGLFGGK
                     KDKNAEIKAEIRGIGEVAEKEAEDAKAAVEEAKESIEQPTDKIAEGEQTAKEKEDEIK
                     EKENQA"
     gene            62887..64293
                     /gene="atpD"
                     /locus_tag="PKI94_00330"
     CDS             62887..64293
                     /gene="atpD"
                     /locus_tag="PKI94_00330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNW25218.1"
                     /translation="METLVKNEGLISQIIGPVIDVEFQQGELPEIYNALKIQKDDGSF
                     VVAEVQQMLGSNKVRTVAMSSTDGLKRGMKVIDTKDSIKIPVGNQILGRILNVIGEPV
                     DEMGPIETNDYLPIHRESPKLVDQNTNIEILETGIKAIDLLQPYQKGGKIGLFGGAGV
                     GKTVLIMELIHNIAMEHSGVSVFGGVGERTREGNDLWNDFKEGGVLDKVALIYGQMNE
                     PPGARMRVGLSALTAAEYFRDFSKQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQ
                     PTLATEMGELQERITSTKDGSITSVQAIYVPADDLTDPAPATTFSHLDASTVLSRQIA
                     SLGIYPAIDPLASTSRVLDPNIIGDEHYNTTKRVLEILQKYRELQDIIAILGMDELSE
                     EDKETVNRARKIQRFLSQPFFVAEKFSGFEGKYIKLEDTIKGFKEIIEGKHDNLPEQA
                     FYMVGTIEEAVEKAKGLN"
     gene            64296..64703
                     /locus_tag="PKI94_00335"
     CDS             64296..64703
                     /locus_tag="PKI94_00335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNW25219.1"
                     /translation="MSEHKLHLKITTHEKVVFDADVDEIYSRSMQGEFGILPDHIPFM
                     CALDIGVTKIVIGSEVQYFAIMGGVFQLKDNQALILAQTAENGKDIDATRAKEAKERA
                     QARLTESSDDIDIKRAEIALARAIIRLKAASGN"
     gene            complement(64700..65116)
                     /locus_tag="PKI94_00340"
     CDS             complement(64700..65116)
                     /locus_tag="PKI94_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNW25220.1"
                     /translation="MTVINEKFVVNTKGHTDIIDITKNVQNSVYRHSLQNASVLVYVA
                     GSTVSITNIEFEPGVLVDLFEALERVIPTGIEYHHDATWHDGNGYAHVRAAIVGNSTS
                     VPLIDGALQLGTWQQIVLVDFDNRPRTRTVHVQIVY"
     gene            complement(65261..65530)
                     /locus_tag="PKI94_00345"
     CDS             complement(65261..65530)
                     /locus_tag="PKI94_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25221.1"
                     /translation="MITASSSAKNDTNGNGKYKPLRKRKKQASSEQVQDFVYLEKIDY
                     RMRFNNSKDPIYFLFDCIEKKSVLQLTMEFIEDSLFEIKNLFAKE"
     gene            complement(65796..67169)
                     /gene="hydG"
                     /locus_tag="PKI94_00350"
     CDS             complement(65796..67169)
                     /gene="hydG"
                     /locus_tag="PKI94_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNW25222.1"
                     /translation="MNIIDVAKINSYLELKGDEKRVNEILTKSLELKGLGTEEIAVLL
                     NLEDENLLNKLFRTAKKVKEGIYGKRIVLFAPLYLSNYCCNNCLYCGFRRDNKEIKRR
                     KLTFDEAVHEAEQIINMGHKRILLVAGEDTNQCNLDYIKEIIDKIYEKKIMNGEVRRI
                     NVNMAPLKTEEFKRLASFGIGTFQSFQETYHPDVYKKMHLSGPKANYQWRLETMDRAI
                     EGGIQDFGMGALFGLCDYRFEVLALIEHSKYLLNKYGVGPHTLSVPRIEPASGSDLSK
                     NPPHAINDKQFKKIVAILRLAVPYTGMILTTRESAQVRREVIELGISQISAGSKTNPG
                     GYSDENSTEQFTMGDTRSLDEIVEELAQHGFIPSFCTSCYRLGRVGKDFMDLAQVGLI
                     QRFCDPNAVATFAEYLNDYASEETKKAGFEAIENHIKNNIKDEKIRAKVIETLDKIQN
                     GARDLYV"
     gene            complement(67260..68123)
                     /locus_tag="PKI94_00355"
     CDS             complement(67260..68123)
                     /locus_tag="PKI94_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25223.1"
                     /translation="MEETSDRMLQSNFNLTNYDFYSSRHEMELVANIVDSLNKIIAED
                     RKEKQPLFLRTTNNFLFDIAKRVVHDKKKTFLIGIAGESASGKTTFVDNTVKACVKEK
                     KEGIYTVIRCDDYFYDTSKELIDAGSYEALFKTGFSFDTPKAVNLDLMKQHLIELKQG
                     NTVVSPNYDFVTCVSKSDGEVKKPAKVILNEGLYVLNEDLREIMDIRVYVFTPIDVIK
                     DRWYNRAATRGKTGEAADMMFADVNHTAQRYIRPAMEISDVIINGMVAVDYIEVITDK
                     IFNAIAEACEI"
     gene            68285..69490
                     /locus_tag="PKI94_00360"
     CDS             68285..69490
                     /locus_tag="PKI94_00360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNW25224.1"
                     /translation="MKKSIKDLGDIKGKRALVRVDVNVPLDENKNVTDDTRIQAIIPT
                     IKFLQEKGAKIILMAHLGRPKGEWNMEFTLAPVAKKLSKVLGQEVLFISTPIGQGAEK
                     DLTELKDGQVALLENIRFYKAEEAKDDDMTFAKELAKLGDFYVNDAFGAAHRNHTSTS
                     KLAKVLKPAVSGFLMEKEIRCLGNALNNPVKPFTAIVGGAKVSTKIGVLENLIDKVDN
                     LIIGGGMAYTFVKANGGKIGNSICEDDQLEVAKEVEKKAAAKGVKIIMAKDVLVTDDF
                     SGNGTNKIVDVYEIPDGFEGVDAGPETQKAFEAVIKTSKTILWNGPVGAFENDKFTQG
                     TKAVALAVQEATKNGAVSVLGGGDTVAAVKKYKLDENTFTHISTGGGASLEFIEGKVL
                     PGVDALDDK"
     gene            69492..71273
                     /locus_tag="PKI94_00365"
     CDS             69492..71273
                     /locus_tag="PKI94_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNW25225.1"
                     /translation="MIELLAPAKDKICAKAAIDFGADAIYIGASEFGARKNAPNSLAD
                     IEEIVQYAHKFNVKVYAAVNTILTDEEIKRAEKLIEDLSKIGIDAIIIQDMGLLSAIK
                     ANNICCHASTQCDNTTAEKVKFLESIGFSRVILARELSIEQIREIKKQTNLELEAFIH
                     GALCVSYSGKCYLSYAIGGRSANKGECAQPCRKKYSLIDEKGNYLAKDRYLLNLKDFN
                     ASNYIKELAEAGVTSFKIEGRLKDENYVKNVVAYYRKLIDDLDYQKTSSGKILFDFEP
                     DLNKSFNRGFTDYFLEGKRKKCFDFDSPKSKGEYIGEVTKIKKDNFEITSPLTLHPSH
                     LFSQDGLCFMYNGEIQGCLVNKVENNKIFPNKMNGVKIGTKIYRNLDSEFEKKLKNSK
                     TQRKIGVNLTFESDKITAVDEDGNITKINYAYEELAKNSIKAKETIIKQLKKSGDSDF
                     EVEDVTININKMPFLPVSKLNELRRELLEKLMKKRLQNYSPIHHPEPISESHQPLNQT
                     INYQGNILNKKANIFYEKHGCKIVESALESGKIPVCGKIVMTTKHCLKSAFNMCKSSE
                     KMFLVDEKGKKYSLKFNCDKCEMEVIF"
     gene            71320..71979
                     /locus_tag="PKI94_00370"
     CDS             71320..71979
                     /locus_tag="PKI94_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW25226.1"
                     /translation="MQNANNYNNFVINLILKSIVDKNFKTLDFGAGYGFFANLLRKSG
                     VSNIKCVEIDEELSEHCKNLDFFVFKNLHDIENNSYDFIYTLNVLEHIRDDKAVLLAL
                     KAKLKKEGKIFIYVPAFKLLYSSFDKKIRHCRRYKKEDLIPFLENNGFEILNAKYVDS
                     FGFFLALIYKFINKNDGEINMLSVKIFDKILFPVGRIFDKIIFEKFLGKNLIVEAKLI
                     Q"
     gene            complement(71969..72955)
                     /locus_tag="PKI94_00375"
     CDS             complement(71969..72955)
                     /locus_tag="PKI94_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LdpA C-terminal domain-containing domain"
                     /protein_id="HNW25227.1"
                     /translation="MQSRQCGLQKLLESRKCFKLICGAGNEDLDEVEKLVAIYAASGC
                     RFFDLSPHKEVIEAAQKGILRVTDKKNLSDYHFCISVGIKGEPHFNKAKINQKFCKKC
                     VNCIDSCLQKAINPDFMVIEEKCIGCARCLKTCKHSAIEIYQKNKPIEDIFFPLFTLH
                     PSPFTCIELHASGTDEKDVQERWNYLNKNFDGMLSICIDRSKLSNEAVLNRIKNLIKN
                     RKLYTTIIQADGNPMSGGEDDFKTTLPAVAMAEIIANEKLPVYLLISGGTNSKTAELA
                     KIFGIDFNGISVGSYARKIVKKYIERDDFWTNKAVFEQASKTAKELIIKIIE"
     gene            complement(73008..73889)
                     /gene="rfbA"
                     /locus_tag="PKI94_00380"
     CDS             complement(73008..73889)
                     /gene="rfbA"
                     /locus_tag="PKI94_00380"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNW25228.1"
                     /translation="MKGIVLAGGAGTRLFPTTMVVSKQLLPIYDKPMIYHPISVLMLA
                     GIKEILIISTPQDLPNFKKLLGDGKQFGVEFSYKEQPSPDGLAQAFILGEEFIGNDTA
                     ALVLGDNIFFGQGFSSMLEGVVRRIKSQGGATVFGYQVKDPQRFGVVDFDKGGKALSI
                     EEKPANPKSNYAVTGLYFYDNKVVEYAKNLKPSARGELEITDLNNVYLKNGNLSVELL
                     GRGFAWLDTGTHSSLLQASQYVQTIEENQGVKIACLEEVALRMKFLKKEDLLEQAAKY
                     KKNEYYEYVARVAQELG"
     gene            complement(73891..74880)
                     /gene="rfbB"
                     /locus_tag="PKI94_00385"
     CDS             complement(73891..74880)
                     /gene="rfbB"
                     /locus_tag="PKI94_00385"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNW25229.1"
                     /translation="MKRLLVTGGAGFIGSCFVRHILNKYPDYKVINLDALTYAGNIEN
                     LDDVKDNLNYTFVKGDICDRTLVRELVEKVDYVVNFAAESHVDRSITGPEIFIETNVK
                     GTLNLLQACKELGIQRYLQVSTDEVYGTLGKTGYFYETTPLAPNSPYSASKASADMLV
                     RAYWETYKLPVLTTRCSNNYGPYQYPEKLIPFFISQLLKGDKVPVYGDGLNVRDWLYV
                     YDHCSAIDTVLHKGRLGEVYNIGGHNEKTNMEITKIILDAMGKDESSIKYVEDRLGHD
                     KRYAIDNEKIQTELGWEPSLTFEEGIKLTIDWYLKNQAWMHSIEDKKAALKGS"
     gene            complement(74877..75434)
                     /gene="rfbC"
                     /locus_tag="PKI94_00390"
     CDS             complement(74877..75434)
                     /gene="rfbC"
                     /locus_tag="PKI94_00390"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNW25230.1"
                     /translation="MPFEFEKQEIEGVILVKPKVFGDNRGFFMETYRKSEFFQNGITC
                     DFIQDNHSKSSKGVLRGLHYQATPKPQAKLIRCSKGKVFDVIVDLRKNSKTFGKWFKA
                     ELSEENKNMLFVPAGFAHGFVVLSDEAELLYKTDAEFCPECDRGLLWNDEEIGIDWGI
                     DFEPILSEKDKIQPKLKELNKEELL"
     gene            complement(75424..75783)
                     /locus_tag="PKI94_00395"
     CDS             complement(75424..75783)
                     /locus_tag="PKI94_00395"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNW25231.1"
                     /translation="MSIGVDIEDIRRFQDKSQDFLDRVYTKSEQEYCLSKKLPAKHFA
                     VRFCAKEAVLKALTGLNISHPPLNMIEVYHIENNFPKIRLLNNLDNKLTIQVSLSHDK
                     TKAIAFVMIDDRGFNAV"
     gene            complement(75861..76688)
                     /gene="tpiA"
                     /locus_tag="PKI94_00400"
     CDS             complement(75861..76688)
                     /gene="tpiA"
                     /locus_tag="PKI94_00400"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNW25232.1"
                     /translation="MRINSIQTNMNFTGKPMVIGNMKMNKSPKETSELINRSAFEMLD
                     LPTHNDIYVGFAPTALAFCKVKEGRNYVDMANIKLGGQEADLGIEKGGAVTGAISQDM
                     YKEAGADFVIIGHSERRGKFAETDKQVNAKTLSALEKGLMPVVCVGESLKQREAGDAD
                     DVVSTQVIKALKNIKEEDITKVVIAYEPIWAINTGKTCDADEADRMCRMIRNEIAKIY
                     GEQNAADVSILYGGSVNDSNADKLFAQSNIDGGLVGGACLDAEKFSKIVHSLVNQKS"
     gene            complement(76753..78033)
                     /locus_tag="PKI94_00405"
     CDS             complement(76753..78033)
                     /locus_tag="PKI94_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNW25233.1"
                     /translation="MRVNLQTQGFYANKNIALVQRTNVQKVNFGNNTELSPAQKLAQT
                     RAYYQHALEKGYAIPAFNVNDLLNVHGIINAVKRTQSPLIIQASKGARDYAGLPYLVQ
                     MVKVAKEEAKGIPVMLHLDHGDTFQVCKECIDGGFDSVMIDGSKFPLDENIRLTKEVV
                     DYAHSKGIFVEAELGKLAGVEDEVSNKTSKFTDPQEAARFVKETGCDSLAISIGTSHG
                     AVKFRPDEKPQLDFERLSEIKKAVEAILPENIGKKPGDAGWRQYPFVLHGASSAPKDL
                     IDMFNQSVVIPRKKFETLENSINDLVNSRAGALTVLSAIEAMKSNTANYFGKGVPEEM
                     YKEATLRGISKVNVDTDFRLGYVGTFKNVAGELDVTDKASIDPRTYGKPIMKTLETIG
                     ARKTDMLNSSGQAMDATDKATKAVELAKNLDIKA"
     gene            complement(78183..78530)
                     /locus_tag="PKI94_00410"
     CDS             complement(78183..78530)
                     /locus_tag="PKI94_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25234.1"
                     /translation="MYVEIEENKLLSWCENPYLDYVYVDIDYATFEPEKYRVIDGILT
                     DISHTQEYAQARIQEQREADIENLKTQIEEFDIKRIRAIAEPEVKDETTEQTWLEYYT
                     QQIIDLRQQLAEL"
     gene            complement(78586..79599)
                     /locus_tag="PKI94_00415"
     CDS             complement(78586..79599)
                     /locus_tag="PKI94_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25235.1"
                     /translation="MSGYLSLIEFVPGTKAKAQEINENFQAVSIALSEKADKAGNSTQ
                     AFNVAAATTSTNAINKSQLDSLSTDLNSKINAAETRFCVKSGNVTNGAGDLLSYSSMS
                     VSFKVGGSYPSLIISNARGTFATFTSVGTLSMSGKPNGAHNVFISTSGNVYSLANNIY
                     KQATRPTLVEGDIWLNTSVEPLKAIKYTSGTDVEFLDIPLGKVTIAGGVITAVETFPY
                     NQNDYDITLNSAIKASTNLAKSITNLPMPNYSSGVSKSWNTSYLAESDGFVMVGCGFG
                     LTFSYSFDGTNWTSNLLFNHSGQGYGAWGFIPIPKGIYYKLNYEYDGSSWKHLKFFPT
                     KGT"
     gene            79747..80640
                     /locus_tag="PKI94_00420"
     CDS             79747..80640
                     /locus_tag="PKI94_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW25236.1"
                     /translation="MKISVIVPAYNTSSYLKQCLESLISQTFNDIEIICVNDGSTDNS
                     LEILNKFAKKDKRIKIISQKNQGLSAARNTGIKAAKGEYIGFVDSDDWADESMFEKLY
                     QNAKKFDADISMCSITTFNEDTGVYNNSDPYMTMNLFPESFEDRVFSPKETYDFIFRI
                     CVVAWNKIYKRDFLDKIGGFFKEGLVFEDNLFFCQTFMQANKICITKENLIFYRLNSP
                     KSITFGDKDFQKLDFFEVFELIEKFLKEKNYYGQLEDYFQNYKKGTLVYWYKKLKNED
                     TKQKYLKKFKKLYPEESLILK"
     gene            complement(80632..80961)
                     /locus_tag="PKI94_00425"
     CDS             complement(80632..80961)
                     /locus_tag="PKI94_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25237.1"
                     /translation="MHEKVEKAVNLLIAEFSKRFDDFHGIYLYGLFLDGNFHEEEDIE
                     LVAVFDSEDKAKRELIWPIVGKVETELDVFIDLNPTTMAELEKDEELYEDVVKNGLFF
                     NPLGQSF"
     gene            complement(81028..81501)
                     /locus_tag="PKI94_00430"
     CDS             complement(81028..81501)
                     /locus_tag="PKI94_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25238.1"
                     /translation="MGEEIVKYFKLSKNCGTEDEDCGSAKIYFNLYTNDEAYNWLGSP
                     YVYKFITLDGMSWAVSVAGDCIEAWHGQSMFGGVINLNLQLCGVAQVDINGMKPPNRY
                     GRDFFSFYITNGNGPLLVPWGSTKIGEDWEWSCGQNFQDDFCTARVIEEGWKMNY"
     gene            complement(81529..81882)
                     /locus_tag="PKI94_00435"
     CDS             complement(81529..81882)
                     /locus_tag="PKI94_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW25239.1"
                     /translation="MTFLVNKTKIQVSFSLGGYLDTKGLRIKIAFTLAEVLIVLGIIG
                     MIADLTLPSLIANFQKEQYVIALKKAYTTFNQALAMMSNDMGCPGDLKCTGLFALGLP
                     PDGIDIETYRNNITP"
     gene            complement(81895..83133)
                     /gene="hisS"
                     /locus_tag="PKI94_00440"
     CDS             complement(81895..83133)
                     /gene="hisS"
                     /locus_tag="PKI94_00440"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNW25240.1"
                     /translation="MTNLIKAQKGTKDILPSEIVNWQCLEKAALDVFSNAGYREIRTP
                     IFEATELFSRGVGDTTDIVNKEMYTFEKSERSLTLRPENTAGVVRAYIENGMHRFPTP
                     VKLWYKGPMFRYERPQAGRQRQFHQVGVEMFGIQEPSADAEVIMLAVDYLKKLGLKNL
                     DVEINSLGCHDCREIYKASIKGAIKPYLKELCPDCQARYEKNPLRLLDCKVESCQKIF
                     KKSEIQKVITSDYICESCSEHFSALKKYLDVLKIKYSVNKLLVRGLDYYNRTVFEIKS
                     NNLGSQNAVCGGGRYDSLVKNLGGEDTPAVGWAMGMERLNSLLPELEPEKLKAYVVSD
                     STQEALKLVQELRENNISADFDMTSKKFGKQLEKASKSALYALILGEDEIKNNKISVK
                     NLAKSEQISVSRLKLINLLK"
     gene            complement(83256..85103)
                     /gene="dnaK"
                     /locus_tag="PKI94_00445"
     CDS             complement(83256..85103)
                     /gene="dnaK"
                     /locus_tag="PKI94_00445"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNW25241.1"
                     /translation="MAKTIGIDLGTTNSVVAVMEGGKPTVIANAEGARTTPSIVGFSK
                     TGERLVGQLAKRQAILNPDKTVASIKRHMGEDYKVKIDDKNYTPQEISAMILRKLADD
                     ASSYLGEKVTSAVITVPAYFNDAQRQATKDAGKIAGLDVLRIVNEPTAAALAYGLDKV
                     KPEKVLVFDLGGGTFDVSILEIGDGVHEVLSTSGDTHLGGDDFDNKVMDWLCDEFKKS
                     EGIDLRNDKQAMQRIKEAAEKAKCELSSVVETNINLPFITADANGPKHLDISLTRAKF
                     EDLTHDLLDRCKKPVENAIKDANLSKGEIDEVVLVGGSTRIPAVQALVKEYTGKDPNQ
                     SVNPDEVVAVGAAIQAGVLAGEVKDIVLLDVTPLTLGIETLGGVSTPLVPRNTTIPVS
                     KSQVFSTAENNQTAVDINVLQGERPMAKDNKSLGMFRLDGIPPAMRGLPQIEVTFDID
                     ANGIVNVSAKDKATNKEQKITITNSSNLSEDDIDKMVKEAEANAAEDKAKKEEAETRN
                     NASGLIASAERIVKDFEGKIDEADKTKLDEQKEALQKALDENKATEEVKKLSEDLQQT
                     MFAISQKAYEQVQKEGETAQNTSSEGEASAQSEASSSDDDVIDAEYTKE"
     gene            85351..86790
                     /locus_tag="PKI94_00450"
     CDS             85351..86790
                     /locus_tag="PKI94_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNW25242.1"
                     /translation="MKIYAINNYANTKLNSTNQNPIGLYSYGLKADSVNFTCSHCEDK
                     KTQRSMPVKKDVRDLGNIEGKTVLMRADFNVSMNKDGSINDSFRIKQAVKTIKYLTDK
                     GAKVVLMSHFGDPAKELKKAKAKNPDLNETEEMAKIQQKLSLRPVAEKLSEFLGKEVY
                     FYSYRTDHLPYNSLQNGEVCLIENTRFNDGETKNDPALARHWGFNTDIYVNDAFGAAH
                     RAHSSTEGVAQYCKKLANPAVAGLLMANEVDMACEILDNPERPFTVVSAGSKLESKIG
                     PINTLIDKMQPEDNQIIAGLMVIPFIINENPNSPLRKLYKNEEELNADLERAKSIVNK
                     CAENGVNLIMSQDVVVQNKDTKEVKTVFVEDIEDGWEIMDVGETTIETLKDTISNSKT
                     VFVNGPVGYFEGGFTNGTKEMLQAIQDLKKNNPEAKTIAGGGDTVTAITNIFGEESFE
                     NFTHVSTGGGALLDFLSGKLPGYLALEDK"
     gene            complement(86862..88127)
                     /gene="gcvPA"
                     /locus_tag="PKI94_00455"
     CDS             complement(86862..88127)
                     /gene="gcvPA"
                     /locus_tag="PKI94_00455"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNW25243.1"
                     /translation="MENFLAHTDETREQLLKEISVSSVNDLFKIIPQQALCAEFDLPD
                     ALSEMQTQKQVKALAKKNNTEMISFLGAGVYNKFIPACVSQVAQRFEFLTAYTPYQPE
                     ISQGTLQVMYEFQSMICNLTGMDVANASVYDGATACAEAILMASRITKREKVLIDENI
                     NPEYKEVIKTYCRAIGIEIEWKITKEKINTGEFACVLIQNPNFYGEIEDINLGEVLTL
                     ANTLLAVCVDISSLSILKAPAEYGAAIVVGDIQPLGIPMSFGGPHAGFMACREKFMRQ
                     LPGRIAGRTLDAEGNQAFTLTIQTREQHIKREKATSNICSNQALIALCATLYLTLLGE
                     KGFKQAGFLSAQNAHKLSEKLSQKGIKTINENFYNEFVIEVENSDKFLLKLRENGIIG
                     GLKLDEKRILIAVTEMNSDEDIELYCKSF"
     gene            complement(88127..88507)
                     /gene="gcvH"
                     /locus_tag="PKI94_00460"
     CDS             complement(88127..88507)
                     /gene="gcvH"
                     /locus_tag="PKI94_00460"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNW25244.1"
                     /translation="MSLTEGILCSRTHEYILEQGDKYVIGLTDYAVEQLGDIVFLELP
                     DVGAEFVKGEVFATIESVKAASEIYMPISGKIVEINDKVVDSPEILNESPFEKGWLVK
                     IQSQNATQDSVDLLEYNDYREEVA"
     gene            complement(88529..89632)
                     /gene="gcvT"
                     /locus_tag="PKI94_00465"
     CDS             complement(88529..89632)
                     /gene="gcvT"
                     /locus_tag="PKI94_00465"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNW25245.1"
                     /translation="MTKKTFLHDEQVKLGAKMIEFAGWMMPVSYSSIIDEHKTVRENV
                     GLFDVSHMGEVFVKGSDALPFLNKLVPQDVSRLTNLKAVYCQLLNKEGGIIDDLIIYK
                     LEENDYLLIVNASRIDEDLNWMVRNSLGFNVSIDNQSHNYSLLAVQGPKASDLMDKMG
                     LKKENQPEFFSIKRAELFNINLFVSRTGYTGEDGFEILVKNEFSELLWNYIIEAGKEF
                     GLKPIGLGARDTLRLEAALHLYGNDMDEQTSPIEANLTWSVSKTKIDDYNGKETIQKH
                     LQTGGDKKLVGLKMLDRAIARHGYEVFYNNENVGIITSGGVSPVLNENIALAYVKNIT
                     EICTGTVLQVMIRDKLYNAEVVKRPFILKKNKI"
     gene            89907..91019
                     /locus_tag="PKI94_00470"
     CDS             89907..91019
                     /locus_tag="PKI94_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNW25246.1"
                     /translation="MGDLKLDKFEINDFLKKSVLMGASDVHLKVDEHPVIRKDGRIIK
                     IDMPKLNDENIENIINVLAPKTIKNKINTVFDIDFSYELPGISRFRVNLSQQLGKSAL
                     VIRNIPYKIKSLEELNLPVSMEQFAQYNNGLVIVTGPTGCGKSTTLACLIDYINTNYP
                     KHIVTIEDPVEFIFTNKKSIISQRQVGVDTPSFHEGVKYALRQDPDVILIGEIRDRET
                     IESALKASETGHLVFATLHTNDAVQTINRIVNLFDPQDRQYIRNQISSTLRGTIAQKL
                     LNKKNSDGRVPALEILVSTPTVKDFIEKDELEQIYSLVKKGSFNEMITMNMSLLKLVQ
                     NEIITKDEAINASENKIELQQMLRGVYHGTAEDGGV"
     gene            91023..91775
                     /gene="recO"
                     /locus_tag="PKI94_00475"
     CDS             91023..91775
                     /gene="recO"
                     /locus_tag="PKI94_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNW25247.1"
                     /translation="MNNFVTDAINLKSYNLSENDKIILMYSKEKGLIKGVAKGCKKPK
                     SKLGARMDCLIANTLMLYKGKNLDTICEAQAINTFKNSRQDLDKLFNSVYISEIVGNF
                     GMENDPASEEIYNLLYKALEKISSAKDKKDILISVIKFQLKFMQLSGFGLELDTCLCC
                     SKPVLEENMYFSSQKGGIICENCKKGEIKLHNKLRDFLTAMLQFDFDYESEYDKKATE
                     KICNVCFELLKDYIQLHCPKKIKTTKIMHEIA"
     gene            91940..93784
                     /locus_tag="PKI94_00480"
     CDS             91940..93784
                     /locus_tag="PKI94_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNW25248.1"
                     /translation="MNQNKQAEDNKKVLYSGNGSSNPIKDGGILARINTLNTRTNLNK
                     VYGGYSHHLYNVSTSVDYSDVLQALNEMTQNKKTLSDVFYSIHSILVNKMNCFFTAYG
                     IYQNQSNYINLKLIDRIGGTYSSKIFSAEENHPIITSYGNRALEFKSDSSFLNIPYLK
                     NSPVVILPLISVDNCIGVLLIGDNNPQANIDFYSLLASHFALFAHNTDLIEKVTETTN
                     TDSLTLLKNHSGFQESFACELKKTQEANTNLSVILMDINNISKINRELGHAKGDEIIK
                     LVAEKVKQNIRNTDIAGRYGGDEIAVILPDTDTSEAKYIAEFLTYSLSCCFVDDVGPV
                     KVSVGISTYPSCSREQEKLLILAEQAMFISQSKGYKDGMSAIVSSQDYNLWDDAALSS
                     FAEVFTKKHAQIGINFEDELINKFNSENIISKNHLIEMVTSLASAIDAKDTYTKGHST
                     SVSRYAEALARAIDLPEKEVERIALGALLHDIGKIGIPEDVLRKPDRLSDEEWNIMKQ
                     HPVIGAEKVLMPNESLRDLIPMVKYHHEHWDGTGYPEKLKGEEIPVAARIVSIADAYH
                     ALISNRPYRKGLSVTKACEILNMGAGVQWDRNLIRHFIQIAPSLSTKV"
     gene            93914..95695
                     /gene="fliD"
                     /locus_tag="PKI94_00485"
     CDS             93914..95695
                     /gene="fliD"
                     /locus_tag="PKI94_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNW25249.1"
                     /translation="MSISISGLGSGLPVDTWIEKLLALKQAKIDEISQEKTFAGYSSS
                     ALSTIKTSFNTLLSSMQKVTDSRFGSTSDLFAQKTATSSDSNILTASVTALASKQTVS
                     VSVSQLASSTVAKSVNTASAKMTGTTTVSSLANGGVTEGNFSVYVNNTKYSISVASTD
                     TLNNILGRINSQTGMSATVTDGKITISDPAQTANIVVGSNSDTTNFANIVALKKNAQG
                     AYVSNQSVLLANTSANLTSANAGFAQQINTSSFKIGNATFNVDSSTTLNGLIYEINNS
                     EDAGVNAYWDTGAGKLVLTSKTTGASNINVQNLSGNFTDVMGLTASTFNPDGSIATTK
                     LADNTQTLGNLATLTINGTSMISSSNTITSDISGITGLTLNLKSINTEETPTTTITIE
                     TNNSSLNSAISDFISNFNEVIANVDEATGSDGYLNGESVLVSIRNNLRMTATASVDID
                     GNYKTLASIGITTGAVGSDINANTNQLQIDSTKLAAALADDPDAVKKLLLGDDTHDGV
                     FKKLESIIDSSMDSSYGYFTTRSATLSENISDLTDTITKKTEELGDYKIELQDKFNQM
                     DQLIATLQNQYSKVISALSTITSNSSS"
     gene            complement(95699..96793)
                     /gene="aroB"
                     /locus_tag="PKI94_00490"
     CDS             complement(95699..96793)
                     /gene="aroB"
                     /locus_tag="PKI94_00490"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNW25250.1"
                     /translation="MLSNELWVDLPSGKKYPIFIDDESIESLGEKIFKHTNANKILVV
                     ISQKVNKLYGNLLNIPNSVKFVLKDGEGQKNFKNFQKIVRFAIKNKLERNDAIVAIGG
                     GVVGDIAGFAAATYLRGIDFIQIPTTLLACVDSSVGGKVAINSNLGKNMVGAFYQPKA
                     VFCNLNFLKTLDERQLKCGMAEVIKYAFIEKNCGVEQDFDLFDFLIANPQRIFSRNTN
                     TLGEIIKISLTLKIAVVKKDEKEQGLRKVLNFGHTLGHAIEKITNYKKFNHGEAISIG
                     MKFAVIVAYERKIIRKSLKVKAFELIDNYKIIKKIPKFNPQKLIQIMESDKKVENGNI
                     NFILPVQKSFVEVFNDITNKEIETCLKLLY"
     gene            complement(96783..97316)
                     /locus_tag="PKI94_00495"
     CDS             complement(96783..97316)
                     /locus_tag="PKI94_00495"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNW25251.1"
                     /translation="MSAEENNKNIVLTGMPGAGKTYIGNKLAKILVHFDFVDIDSEIE
                     KQAKMNICEIFEKFGQDYFRKLEQEIIEKFSKLKNQIIATGGGTCQSPKNVLNLKKNG
                     IIFYLSATSDELFSRIKNETKRPLLNVENSLEEIKKLLSKRRRNYLKADFVIDTNKKQ
                     AYTILDSILKEYEGHVK"
     gene            complement(97318..98382)
                     /locus_tag="PKI94_00500"
     CDS             complement(97318..98382)
                     /locus_tag="PKI94_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNW25252.1"
                     /translation="MLKNFRFLTSGESHGQCLNAIIEGVPAGLKIDFDFINSELSRRQ
                     QGYGRGERMNIEADTVEIKSGLRFGKTTGAPICLEIKNKDFENWQKIMSTEEHDFTDE
                     KSFSAPRPGHADFAGAIKYDLKDLRDVLERSSARKTAVDVAVGAVAKIILKEFGINCF
                     SKILHIAGESEEEKIKEKVDWAKGQGETLGGKFEVIFENLPVGLGSHVHWDRKLDGLL
                     AQAVMSIPAIKSVEIGLGEKSSQTLGSQMQDEIFFENGKYLRKTNNAGGIEGGISNGE
                     NIVIKASMKPIPTMKKALSTVDFKDNSQVESYFERSDTCAVEACCVVAEAMSACILAD
                     EFLEKFGKDSLTEITKNYRG"
     gene            complement(98451..98915)
                     /locus_tag="PKI94_00505"
     CDS             complement(98451..98915)
                     /locus_tag="PKI94_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25253.1"
                     /translation="MKKILTVFTIMMFGLCTFADGQEDALKFFNSFVNAANSYSQAVP
                     SMYSDNAKIIRQIVKPDGSLANAYFTADQYRAQMRLSEKIAKLKNYKNYYSNIKVTKV
                     SNGYRIDSLRRPSLSNYYLKSSMVVQKQSDGRWLIVEELMQTKEQIFLKYAK"
     gene            complement(98981..100024)
                     /locus_tag="PKI94_00510"
     CDS             complement(98981..100024)
                     /locus_tag="PKI94_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNW25254.1"
                     /translation="MTDVITIGSATMDVFVECEDANIVSVSTKNSKSEFMSYPYGAKM
                     EVSAFASNVGGGGVNTALNFANLGFNTSAICKIGDDIYSKGILQALCCKPNLDLSNII
                     QDNSVSTGFSIILMSFQGDRTVLAQRGANATLKKSEINFDAIKNAKLLYIAPLSGESN
                     RVLQSIVEFANENGVKVCFNAGTTSLKRGFEYIKKIIDTAQVAVMNKEEAMMCTKIQV
                     RPDNRNENFSKELIHPDIKEMLKKLKVGDEQIIVITDGKNGVYAYDGKTFFQCPPFPV
                     QIVSTLGAGDAFSSTFCASLTKTGFDIGKSLMYASVNSASVVSKFGASEGFLTFDEIE
                     EKLKNNPDYHYNI"
     gene            complement(100114..101028)
                     /locus_tag="PKI94_00515"
     CDS             complement(100114..101028)
                     /locus_tag="PKI94_00515"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNW25255.1"
                     /translation="MGLDAKSKQYLEDFKSYLMVEKNFSKHTSKAYCSDVLSYLLWLG
                     DISCLSVNFSKVREYLHFIQRFDYKKTTIARKIASIRTFYKFLYRERLIETNPALNLS
                     PPKRPKSLPKFLTTDEIEKILNGVKIDTPAGFRNKAILELLWATGMRVSELSGLNFGD
                     LNLDNNEIKVFGKGAKERIVLVSDRAKTYLQRYLDTARLLVAKGFPLEQIGDSVPVFI
                     NNTGYRLQNKTIRNVINEVVNSLELPKKVTPHVFRHSFATRLIENGADLRVVQELLGH
                     ASISNTQIYTHVSTQRMKQVYDEAHPRA"
     gene            complement(101184..102554)
                     /locus_tag="PKI94_00520"
     CDS             complement(101184..102554)
                     /locus_tag="PKI94_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW25256.1"
                     /translation="MKKIISITLSIAILALNAMPVLAVKNKEIKNKRVIQSQVSEYKF
                     KDVNLEWWQGYNDEYLQGYIIKAIKNNYDLKIATLKVEEARQSVKAQFSRELPTASVG
                     FAPALYKMNGVSNTEVSYGFPLMVNYEADIFLKNRDKTKAIKKYYEASQFNEKTAYIS
                     VASAVGAAYFNLVKMDKLIELQQQIISDRKEIYDLMKIRNVQGITSTADLMRAEKAYV
                     LAQTDLSDLQKAREIVLNHLAVLIGDSPDNTKEFKRISYDEMNYKLNIPNEISSEVIT
                     QRPDYQAAEKMVEKAGIDVRVAKKEFLPKIDIIGLLFFNTSSLGASFDWKNALAIMGG
                     QALLPLFTGGARIANLKIYKNRYEQILQNYQKTNLVAIQEVNDALSSLKLDNDKYNNN
                     LKAYQMQEKDYGYMQKRYEQGVISGLDLIQQKETLLVLNKMVVSSKTDCYINEISLYK
                     AVGGKL"
     gene            102759..103226
                     /locus_tag="PKI94_00525"
     CDS             102759..103226
                     /locus_tag="PKI94_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNW25257.1"
                     /translation="MKEKETTNYFNSSIYLMEQVVTYAKIKGTQFFKELKMGVTIDQF
                     VALDAVYSSNDICQRDLSKLILKDRSNTGRILNILELNGFIKRVIETKGKRLVKKIYI
                     TQSGKKLVEESIPKLRSAFLQVFENISDKDFMILRETLEKMKLSLSKTTSIQI"
     gene            103240..104211
                     /locus_tag="PKI94_00530"
     CDS             103240..104211
                     /locus_tag="PKI94_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNW25258.1"
                     /translation="MSNEKNSKLMKKRFLIPSITIIVAILALIGMINSSRYQSTEDAY
                     VETHTVAVAPKVSGQVIEMYVNDNQHVNAGDLVAKIDSADYGAKLDEISAKYEATLLK
                     QKNAKAVYEAANSQITLAKKNLDRYTELYKSGAASKLEFDNAKTNYDNAQASLTQAHQ
                     NLLSKENNRVADADLKQLAATKKQAQLAYSYTNIYAPQSGFVSNKTIAKGAIVQAGQP
                     LFTLVPDDIWIVANFKESQLENMKVGQEVEIKVDAYPHKRFKGKIDSIQRISGAKSSL
                     FPPENAVGSFVKIVQRIPVKIVFAEKIDTSKYTIVPGMSVEPKVKVK"
     gene            104220..105809
                     /locus_tag="PKI94_00535"
     CDS             104220..105809
                     /locus_tag="PKI94_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNW25259.1"
                     /translation="MSENINNLKTEEWKLPENPWPATFAVMIAVFMFVLDSTIANVAL
                     PHMAGSFSSSNEEAMWILTSYMIASGIILPSVAWFSGVFGRKNFFIGCVIIFTVSSFL
                     CGLAASLDQMIIARILQGLGGGAIVPVAQAILIENFPLEKRRMAMSVFGVGIIIAPIV
                     GPVLGGWITDNYNWHWIFYINVPIGFLAALMANMFIEDPPFARKKEGQKIDFLGFSFL
                     IIWLVCLQIVLDKGNNADWFNSPWVCWTFAASMLSMVAFGVSQFVNSKSIIDLKVFKD
                     KNFTFGTIFMVLVTAVLYSSIAIMPLFLQNLLRYSAYLSGLSMMPRGVGSVTGLIITG
                     IFAKKIDDRLLIAIGLALMGVSSLMFGFLNLQISMINIIIPNFIFGLGMSCCMIAIST
                     ISVVTLRAEQMTNATGVQNLLKNIGGAVGMSIVATFLSRFAQVHQNMMVGNLNPLNPA
                     FQYKVSAMTAAFSKYFALPLAQQKANYLMYVELLKQSSLWAFMDAFRIFGVICFIIMP
                     LLLFMKPHKSNSTEDGFSAMH"
     gene            105886..107829
                     /locus_tag="PKI94_00540"
     CDS             105886..107829
                     /locus_tag="PKI94_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25260.1"
                     /translation="MKKKLIALTFACTLAASTTGVFAQEDSSAPVQVHPAKTDNIKAF
                     PENLPQNPTVGQGQTLSTKIQYSKFRKVHKKVTKKYVNYDEVDKLIENSYYEDADKIL
                     KTAINSNSKNIKAKALWVVSLAKQYKLDPAQTQLNELLKKYPDNADLHYAQGIIYYKR
                     TTSSNMEHIKNSQKLFEDSLKEFNKAIELDKKDARYYNAAGVVSLQLGKDADARKLFA
                     KAVELNPKYSTAIDNMGTMDFVDGKLEDAEKKFNQALLHNTQNTTAMYHLAQVANQRQ
                     QYSNALTYLNNALAINPNSYAFQNLSGEIYAKQGNEAAAINAFRKAVMIKPEFAPAYF
                     NLAEVYEKRGDGEFAIEQLKTALAIDPTFYDAKLKIADISLASGKYNQAIDNYSALAQ
                     IDDYKNDALKGLANAYFAKAQISSNKSVLGSSQELYKAFDELNNAIKANNDDLELHLA
                     KLKLAKVINQPAMSQDELQNIINSNDTTLTAMVVKGEAYMTLNNSPEAKKMFDAAAAC
                     AKTLEDRQYLTEIFIYNKQYESAQAIIDNILKSDPQNTQALANSDYIKKCEKYSQNSL
                     KSAQYFLKSKNYPTAQEYATRAIGLNPNNSQGYVILAEACEQQKDYNSAIANYKIYLN
                     MTPTSADKNKIQNKIKYLENRFK"
     gene            107955..108227
                     /gene="rpsT"
                     /locus_tag="PKI94_00545"
     CDS             107955..108227
                     /gene="rpsT"
                     /locus_tag="PKI94_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNW25261.1"
                     /translation="MANIKSAKKRILTAERNAEKNTAFKSSIKTAVKKVLELAQGKDK
                     KALDEAVSKVYKLCDKAVVKGILHKNTAARKKSRLSLALNKLSAKK"
     gene            108300..108830
                     /locus_tag="PKI94_00550"
     CDS             108300..108830
                     /locus_tag="PKI94_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNW25262.1"
                     /translation="MLTVSKINSVPISFKKLILPPKNYKVIDEYVSRSAQPDKESFLW
                     LKNQGITDVINFRTMYEPKIDFDESQYLKQINLNYHHLPTGTRTISELTVKKFIELIE
                     EIRNPNSKRKVHLHCKAGADRTGFFTMIYKGLYNIDSFENNAKEMLEMGHNQGLFPNL
                     INSAKKMIEFFKKIKA"
     gene            complement(108827..109633)
                     /locus_tag="PKI94_00555"
     CDS             complement(108827..109633)
                     /locus_tag="PKI94_00555"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNW25263.1"
                     /translation="MNRFFLTIVALFCITMISCGFCVSEPVQIDYQNGIYHITLDGKR
                     VQKGIKFVSSEKLMTNKEAHETSRSKFTINTGFFDPKNQKTISYIVTDRNISADPLFN
                     ENLLMNPVLRQNLDKIINRTEFRVVDCDGKINYEIVPHKTNLDFACSVVTSAQGGPML
                     LPELRLEEEFFILKKDGKIVRTSASVLDKTARTIIGLKGQDIHILIITNDNPMTIYEA
                     RDLCQQLGFEKAMAFDGGSSTSMNYKKSIEVVSTNDSAGRLLKSFMIVNY"
     gene            complement(109711..110826)
                     /locus_tag="PKI94_00560"
     CDS             complement(109711..110826)
                     /locus_tag="PKI94_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNW25264.1"
                     /translation="MKLFSKIKLYDKYVFEQVFWATMVSILLFTVIWIAPEMLLNTIR
                     KTLEGEYTTKTAILVLFYELPNILGKALPVGLLLGSLFTFDKLSKDFELTIFRVAGLS
                     FWRILSPVIVLSLLITAFCFVTYDKLIPFSASKLVGIKREYSPSQYIYTQKDEKNRPT
                     LAVIVSRFNYSNIANVIVLDFSEKTYTDLHALSHIFVAKNADHYTEKWILKDVTEYRI
                     SNDGIFEEIKHLNNLELLQGKAAQNAYDLMIYSLKRDREIGNGDLRRYIKLLKQEDLQ
                     EEYRFMLNKYFQRFFHPFVCVLLAILGCLLGFSKPREQRLVGFTIAIGCIFLYYITLP
                     FFDLLAEKGVLHPFITASFPLIAFTCAIWAFYKSKDL"
     gene            complement(110826..111551)
                     /gene="lptB"
                     /locus_tag="PKI94_00565"
     CDS             complement(110826..111551)
                     /gene="lptB"
                     /locus_tag="PKI94_00565"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNW25265.1"
                     /translation="MKITAKNLVKIYGDRSVVNDVSFEINKGEVVCLLGPNGAGKTTT
                     FYMVVGLVKPNKGHVYINDQDISAWPMNVRAKAGVGYLPQEASIFRKLSVEDNIKLVL
                     EMNEKLTVDEKKRKLEELLTEFGIIKLRGESAVSLSGGERRRVELARALAASPDFILL
                     DEPFTGIDPIAIGEIKENIRKLSDEKGLGVLITDHNPKATLSITDRAYVIFDGKIKIE
                     GKSEDVANDPIAKEFYLGKDFEL"
BASE COUNT        35882 a        20958 c        18716 g        36064 t
ORIGIN      
        1 aagaattaaa aatgtacgaa aattattttg attacattat atgtggtgat gttttagaac
       61 atctatataa tcctcttgac gtaatcatat gtatgaaaca ttttttacaa agtgacggac
      121 actttataat ttcattacca aacatagcac atggaggcat aaagttaaat ttattaaaaa
      181 ataaatttaa ttacaatgat tatggcttgt tggataaaac acatattagg tttttttcat
      241 taaaaagcat tatagatttt atgacgaatg caagcctcga aataattgat attaatatgg
      301 tggttatgcc aatcgaaaat tcaattgaaa atgttcagga aaaagaacat cctattgaaa
      361 tagttaatta tattaaaaaa gatattgaat cttatgttta tcaatatgta attaagtcta
      421 aaatttctaa gctagatatg caaactttat ttgaaaaaaa ttttaaaaaa actttattat
      481 ccaaagaaga aaaacaaatg ttgatgcgaa atctgatttt aagtgaaatg cctcacaaaa
      541 aattatgggg ttcgaaatta aggccatttt taagaaattt aaagaaaaaa tttttacccc
      601 cccccgtcat aaaaacccaa aaaataccaa atacaacaaa tattaaaatg caaaaacttg
      661 ttagaaaatt tttgcaaatt agtaataaat atattattaa aaacattaat agaatattat
      721 ctaaacttaa aaatcccctt tttttaaaaa ccttatatat taatgctagt gaaaaatcgt
      781 ctgaatatgt taatggttca aaaataaata taaaattaac cgatgatacc atgaaattga
      841 ttgcttttta tttaccccaa tttcatccta tagatgagaa tgataaatgg tggggaaaag
      901 gctttacaga atggataaat gttacaaaag ccgttcccca atttttagga cattatcaac
      961 cacacctgcc ggatgaactt ggcttttatg atttaagatt aaatgaaatt atgtatagac
     1021 aaatagaact cgccaaacag tatgggatat atggattttg ttttcattat tattggtttt
     1081 ccggcaaaag attattagaa aaaccgatat ttgatttttt aaacaacaaa gaattaaact
     1141 ttccgttttg tttgtgttgg gctaacgaaa attggacaag gcgttgggat gggcaagaca
     1201 atgaaatttt gatttcacaa actatggaag atggcgatga tattaaattt attgaagata
     1261 ttataccttt tttcaaagat tccagatata taagaataaa taacaaaccg gtattaatta
     1321 tttacaggat acatctttac actaaagaaa aagtacaaaa aatagttcgt aattggagaa
     1381 attattgcaa acaagtcggc ataggagact tacatttaat tgcggcactt acgcatggtt
     1441 tttttgataa tccttccgaa tggggtctag atgcagcaat tgaattcccg cctcacaata
     1501 tgaatttgcc aaatataagt aattatctgc caattatgaa tccgaatttt aaaggtgcta
     1561 tttatgattt aagtaaatat tttcaacaag acaattactt gaaaaaaaat gattttacaa
     1621 tatatagaac tgttttccca aattgggata acacagcaag actccccaat aatggtagca
     1681 ttttccttaa tgcaaatcct gaaacatata aaaaatggct ttctgatgca attaaattca
     1741 caaaaacaaa ttattcaaaa gaagatcaat ttgtttttat aaatgcttgg aatgaatggg
     1801 ctgaaggtgc tcatcttgaa cctgatgcaa aatacggcta tgcttatcta aacgaaacga
     1861 aaaaagcctt agaaaatcaa tttgataaaa attaatttaa ctatagccaa aaagtctttc
     1921 ttagaaaaaa caacgggtct ttcttaatat atttaaatat ttttaaaata attgttctga
     1981 tattgtttga actagaacgt aatttatcat tagcataaac gcattttatt ttagataatt
     2041 cactaaattt attatttaaa atttcttttt tataaccctc ataaagcctt tttaaaacat
     2101 cattatcttc aaatgacact ctggtataat tttctgacag tacatctgct ttattgaaat
     2161 tataatcact aaaatgataa aaaattaagt gatatttatc atttataaaa tatttatccc
     2221 cctttaatgt taaagtgcgt tcatgtaaat tccaaggcgc aacatttaaa ccccaatttt
     2281 cagaaacctt tacacgattg aaaaatatcg gtgcaaagtt cattggaagt tgatcaacaa
     2341 aaataccctt tgttggtttt atataacaat taacttccaa tcttctcttc caccaatcaa
     2401 gtatttcata gacatcttca cttcttttta aagccaaaaa tcctaaatta taagttccat
     2461 gttgagtaaa atcattttct acaggtgttt taccatcaaa ctcaataggt ttataaatat
     2521 gaggagttaa aagaattgaa ctctccataa gttctttttc tattgttgct aaattatcaa
     2581 agacgtaagt gtcggggtca aaatacataa tatattcaac atctttataa tttttaaata
     2641 aataatcaaa tacaaaaggt ttaatagatg tatttaattc aattatatta tatttattgc
     2701 acatttgctc aaatttttct atttctaagt tatgtgcttc aatcacccta taaggttcaa
     2761 aaaatgaata atcaatatct ggatgtttta tgtcacataa acatattata aacttataat
     2821 cggaattatg ccttaaaaga ctatccccga gggcttttgc ttttgccaga taattatttg
     2881 aacatattgt aaaaattatt ttcattatgg tattatctta taattataac ccttagtagt
     2941 caagcttact tttatgctaa aataatttaa taaaagaagg aaatatttat ttgatactta
     3001 attttattga tattttaaaa agaaaacatg caaataaaaa gcaaaggcaa attattgaaa
     3061 actttcacac tttatattac aacaattctg aacgaacttg gcgtaacaca aattggcttg
     3121 gtataaatgt tttaaaatgt cctttggatt tatggattta ccaagaaata ttatttgaag
     3181 tcaaaccgga tttaataata gaaactggaa cttatgttgg aggaagtgct tattttttgg
     3241 cttcaataat ggatttatta gagaatggcg aaattgttac tattgacagc gaaatacgag
     3301 gaaatttacc aaaacataag agaataaatt atttaaacgg tctttcaact tcaatagaaa
     3361 ttattgaaaa ggtaaaaact tttacaaaaa acaaaaaaag aatattagta attttagatt
     3421 ctgatcattc taaagaaaat gttctaaaag aaatgaggat atataacaaa tttgtatcaa
     3481 aagatagcta tttaatagtt gaagattcaa atataaatgg gcacccgact cttcccgaat
     3541 ttggaccagg cccatgggaa gcaataaact cctttttaaa tgaaaataaa gagtttaaaa
     3601 tagataaaga aaaagaaaaa ttttatttta ctttaaatcc taatggttat ttgaaaaaac
     3661 aataattaca cttaaaaatt ataaattaat ctatgaatgt aatacgccgt ataatttatt
     3721 cgcaacagga ctaagttgta caaaaaaact ccccaggttg gactcgaacc aacaacctac
     3781 cggttaacag ccggttgctc cgccattgag ctactgagga ataatttcta cttcggtctt
     3841 tgtagattta cttttattta attgtttttc atttaccccc acgtgcttct cacgctctgg
     3901 agctactgag gaataccttt caaaatttta acaaaaaaaa cttttcttgt aaagtatttc
     3961 ttgacttagc gagtgctctt tctttatcta atcattaatt tttattaaaa accctcgatt
     4021 tttccgcaaa aatattataa aataaattat ctttactaat aaaagggggt ctctatggaa
     4081 attcaaagta taaactctgc agaatcaggg tttgcaaatt acgataatct tgaaccaaat
     4141 gataaagcaa gtatccaaaa atccctacaa aatctgggga tacctttaat tataattaac
     4201 agcagtgccc gagcaattaa aacttatgcc acggagcacg gaatcaattt gaaatcaata
     4261 tctgcaagaa ttgatgccat gataaaagaa caacaggcaa aaacccctgc tcaaacgcct
     4321 caaaaagatt ttgaagacgc acttttttta tctgggatac cacaagaaat tataaataaa
     4381 ggtcaaaatt ccattgaacg gtatgcaaaa gaaaaaggaa tagagttacc aaaatctccg
     4441 gacggtactg cttttaacaa atcgttttag ataaaacata gggcaaaaat tttgtggcgg
     4501 atttttctct gaaaaatccg ccctttaaaa ttaaaaaact tatccttaga tattcgacaa
     4561 tttaaccaaa ttttcaaaat cagggaattt ttttgacaat tccgaaaaac ttcctgtggc
     4621 aacaacttca ccgtttttca aataaaccaa tctgtcgcag cttttcagcg tcgaaagcct
     4681 gtgtgctatt gcaataattg ttttttcgcc ttttagatcg ttgagcattt ttgttatttc
     4741 gtgttcggtt tcaacatcaa gcgcggacgt tgcctcgtca aagataagca cctgcggatt
     4801 acgataaatc gctcttgcaa tcgccagcct ttgtttttga ccttgcgaca ttcccctgag
     4861 acctgaggtg attttggagt caattccctc ttcaaattcg ccgaccaaat caatcagctt
     4921 tgctctttca agaacttcca aaactctttt atcgtcaata tcggattcat ttactcccca
     4981 tgccacattt tgcttaaaag ttccgtctaa aatgtttatt tgctggggca catagccgat
     5041 tattttcctg agtgcaacaa aattttccga attaagcgtt attccgtcaa ctttaatttc
     5101 ccctgcatca acaggcaaaa gtcccataat aatatctgca agcgtgcttt tgcccacccc
     5161 tgagagccct ataattccta taaattcgcc ttttttaatt tcaaggctta agtttttaat
     5221 aacttttctg ttttcgctgt aagaaaaatc tatatttttg agctcaattt tatctttaaa
     5281 atcaattttc aaatccgact ttttctcaac tgaagaaaag tcacaatgct cgtattccgt
     5341 aattaaaccc ctaacaactt ctcttgaagc attcatggca tttagggatg tttgaatttt
     5401 gttaagggca ggcgcaatcc tgaaaatcgc cgcaaccgta atcgcatagg atgcaatcat
     5461 caccgaagaa ttttgcgctg tctgaacaga aattattgct gccagaatca aaagcgaaac
     5521 gacaaccaaa atttctacta tataaggcgg aattgaagca aaaaagttat tttgaaaaac
     5581 agctttgttg aactcttttt gggttttaag atattgttca taaaagtaat tttccgaaga
     5641 taaaattttc agttctttta aattgtttat gttgttgaga acctgagagt tgctttccaa
     5701 agacacttca aggagttttt tggaaagttt tttcatcttg tttttaaaaa atgcgctttg
     5761 aataaaaagg ctcaatccca caaaaacagt tgtaacaaga gccgcaaagg gaaattttat
     5821 gaataaaagc agcaaaatca ttgccacaat tataaaattt gtcgcaaaat taaccgccct
     5881 gaatacaaat ccgtctaaag actgcggaat aaggaaattt aatgtgtaaa gtttttgtgc
     5941 aggctgggtt ttaagtgcca ttttataagg cgcaaaaaga taatattcca taaactttct
     6001 gctgatatca aatttccagt tgttagcaaa tttattttgc aaataaacga cgcaaatcat
     6061 aaagatattc ttaagaataa acagcaaaac aacaaaaagt cctataataa aagaaccgac
     6121 cagagcgtct ttaatttgaa aaatttcact gaatttaatg taataacttg aattgataac
     6181 gctttgaggg tttattatca acaaaacgaa aggataaaca agtgctattc ctataaattc
     6241 aaacaatccg gcaatcaaag ataaaataaa aaagcccaaa agccttattt ttcttccttt
     6301 gccgaaatat ccgataaatt ttcttaaatt accgatgaac ataaaacaaa tttatcataa
     6361 aaatcataat tgttgtataa tatgggaaat agtaaatagt aaatagtaaa tagtaaatgg
     6421 taaatagaga gggcaaaaat tcaagtaaaa acttttcacc ttttcagcta ttcacctatt
     6481 cacccaataa aggagggaga tatgacaaat ccggtattta accaaaacgc aatcgacaga
     6541 gagcgcgtac ttgattcaga accgatgacg gtaaacggag ctataaacaa aacttttata
     6601 ctgttttgtc tgctgctttt atccagcctt gtggtttggg acatgttttt taaaggctac
     6661 actgataaag tcgcactttt aactatggta ggctttattt caagcataat agcgtttata
     6721 gtaataatgt tcaacagaaa cgctttgcct attgcagcac ctgtttatgc ggcatcggaa
     6781 ggtttgcttc tgggcggaat ttctgcctat atagataaag cgtatccggg aattgcaatt
     6841 caggctgtgg gtttaacgct tatggcactt ttttcaatgc tggcacttta tagggtgggc
     6901 gcaatccgct gtacggataa gttcagaagc gttttgttta tttcaacgct ttctattgca
     6961 gggatttatc tggtaaactt tatcggctcg tttttcggga tgcacattcc tcaaattttt
     7021 acttcaagta atttcggaat aggcttcagt attgttgttg tagcgattgc ctctttgaat
     7081 ctgataatcg attttgattt tatcgagcag ggcgctcaaa accttttgag caaaaatttt
     7141 gaatggtacg gcgctttcgg gcttatggta acactcgttt ggctttacat tgagatttta
     7201 aatctgctgg caaaactccg cgacagataa aatgatattg ttactttttc ttatataatt
     7261 tttctttcgg tactttttgg ttggggccca cccttttgat taagtatcaa aagggaaggg
     7321 ataaaagtac cacatagcca ctattgcaaa caaacatatt aagcggaata tttttgttgt
     7381 tccgtgtcgc cattttctac ggctcgattt gtcatcccgc acctgttgcg ggatctaatc
     7441 tcgccttgaa aagaaggcaa agccacggcc tacctgcgtt cgctaattat tactaaactc
     7501 aactcaagct cgcgaactcg tgccttaagc actcaaacac actcgctttg cagttgtttc
     7561 gttaagtaat aattgctcac tccgataagg tcggaagggg gttaggggca cagccctgac
     7621 gctttggcat tttgttcgcg tgtttttttc ttttctttcg gttcggtttc ttttcttttt
     7681 ttcaatcgca agaaaaaaag aaaagaaatg gaacaaaata aaactagcct atttaaattc
     7741 aaaaattaat aaatattata ttttaatact tatttcacaa ctatattaac aagttttttg
     7801 ggaacgacaa tcactttgac aatttcattg ccggcaataa attcttttgc cttttcgcaa
     7861 tttagcgcaa tttgtttcat tttttcctgc gataaatctt gcgccacttc aactttgtcc
     7921 ctaacttttc cgttaacctg aacaaccaaa gtaattgagc ttgttttggc taaattttca
     7981 tcccattcac accatttttc atcatggata gaagtttttg cacccaatga agtccaaatt
     8041 tcttctgtca aatgcaccgc aacaggtgac atcaatttca tcaaagtcaa aaccgcaaag
     8101 ctgaagacgt ttttatcttc ttgtgaaaaa tcttttttct tgcctaaata atcgtaaata
     8161 gcgttagtca attcacgata cttagaaatc accgtattaa actggaattc attttgaata
     8221 tcgtttgtaa ttcctttaat catgatgtgg gtaaatctta ccaaatcatc gttttctttt
     8281 gatagatttt cgctcaattt ataatcaaaa ataattttgt cctgattggt tgaaactaat
     8341 ttccaaacgc ggtttaagaa tttataacag ccttcaacgc ccgcatctga ccagtcaaaa
     8401 tcccttgcag gaggcgaatc ggagaggata aacagccttg ccgtatcagc cccgtagttt
     8461 tcaaaaattt catcaggatc aaccgtattt ccttttgatt ttgacatttt tgagccgtct
     8521 tttaaaacca ttccctgagt taaaagattt ttaaaaggct cgtcaaagct caaaagcccc
     8581 atatcacgca ttgcttttgt gaaaaatctt gaataaagca agtgcaaaat tgcgtgctca
     8641 attccgccca catactggtc aacgggaagc catttatcga caatattttt atcaaaacac
     8701 ttttgactgt ttttgggatc agcgtatctt agataatacc agcttgaaca aataaatgta
     8761 tccatagtgt cggtttcacg ctttgcatca ccgccgcatt taggacattt cgtatcaata
     8821 aacgttttgg atgtcaaaat gggcgatttg cccgtaacct taaaatcaac atcttccggc
     8881 aacaagacgg gcaaatcgcc ctcatttacc gcctgaattc cgcatttatc gcaataaaca
     8941 atcggaatcg gtgcacccca gtaacgttga cgagaaatca accaatcacg cagacggtat
     9001 tgggttttaa attcgccaaa acctccgtca gcggcttttt gagtaattat ttttttgaat
     9061 tcttcgttac ttattccgtc atattcgccg cagtttacca tagtgcctga ttcagtatat
     9121 gcaacagtta aaccactaaa agaaacgtca tcctgaactt gtttcaggat ctcattattt
     9181 gcgatgactt ctttaatcgg caaattatat ttttttgcaa agtcaaaatc gcgctcgtcg
     9241 tgcgcgggaa ccgccataac cgcccctgtt ccgtattcgg ctagcgcata gtccgctgtc
     9301 caaagcggga atttttcgcc cgtaaaagga tttatgcaat gcgttcccaa aggcacgccc
     9361 gttttaattc tttctgtcga aagcctttcg atttctgtca ttttacgagc gttttcgcgg
     9421 tatctttcga cttcttcttt gtattgaggc aatgtaagtg cttcgatgtc tttgtattcg
     9481 ggggctacta caagataagt tatgcccaaa gccgtatctg gacgtgttgt atagacagga
     9541 atttccatgt catattctgc gactttgaac ttcaaaatcg cccctgttga ttttccaatc
     9601 cagtttgact gcatagtttt aacgttttcg ccccagccgt tgagtttatc caaatcttca
     9661 agaagcgctt cggcataatc ggtaattttc aaaaaccatt gggataaatt tttctttgta
     9721 acaacttcat cgcaacgcca gcattttccg tcgattacct gttcgtttgc aagcactgtg
     9781 gcacattttt cgcaccagtt aaccgccgcc tcttttttgt aggcaagtcc gcgtttataa
     9841 aattgcaaga aaagccattg tgtccatttg tagtaatctg gcttgcaggt ggtgacttct
     9901 ctgtcccaat cgtaagaaag accgagcatt ttaagctgtt ttgtcatata ttcaatattt
     9961 tcatcggtcc actgtgcggg atttgcacct ttttgcatgg cggcgttttc cgcgggaagc
    10021 ccgaaactat cccacccgat aggatgtaaa acgttgaatc cttgagcttt tttaaaacgt
    10081 gcaattacat ctgttatggt gtaatttctg acatgcccca tgtgaagcct gcctgaaggg
    10141 tacggaaaca tcgaaagcgc ataatactta ggtttatcac tatcggtcgg agtggcaaaa
    10201 catttacatt ccccccactt tttttgtaat tttttttcaa tttcctgcgg aaaataatgc
    10261 tctttcaaat tattgctcct caactaatct tttgttaatt aaattttagc ataaaaattt
    10321 tttaaataag gaaaaattaa tcttgaagaa tattaaaact ttaaaaaact aagggccaaa
    10381 aggcgctgct ggtgttggtg gaccgggagg accgggaggg ctaggcggat caggaggagg
    10441 tgtaacagaa aagtcctatt ggcaggcaat gcaacccccg ggagggctag gcggatcagg
    10501 aggaggtgta acaggatctt cttcttcctc ttcttcagga ggatcttcag acaaaggagc
    10561 tgcggccaaa cttgaaatat tagcaagata gtccattttc caaccttctt ctattattct
    10621 tgcagcacaa taattcccgt cagtatcgct tggaaaacaa tgctgagccg tatcaccatt
    10681 tttccaatga ccattttctt gatcgtctat tcctccactt ggatataaca aaggtccctt
    10741 accatttgta atataaaaac gaaatatatc tttacccata ttattgggag gtttaagccc
    10801 atttaaatca atatacaatt cgccacaaac ttgttccata ttttgagtta cgcccgtgct
    10861 catagaagat gaacagttat tatgattatt ttttaaaata aaagcaattc catccgcact
    10921 aatgaaacgg taaaacccgc ttgaaatttt tgcatagtca tctgttctgg ctaaacttcc
    10981 atcgtaagaa tcgctaacac tttcagaaaa acaacctaga gtatttgttt cacaattttt
    11041 caaaacgtta aaatgcttaa caagctcagt accaagcgtt tggctagtag tccccacctt
    11101 aaaaagaccg gtgcaaatca aatcaccggt gcaagtaaaa tcatgagccg ttcctattaa
    11161 agcttgatta aattgagtat aagctttttt taatcctgac aaaaattttt gctgttcaaa
    11221 ataccccaac aaaataggaa ttgtaactgc ggcaattaaa ccaataatta aaagagtcaa
    11281 caagacttca ggcaaagtaa aagccggttt cttttttgtt tttaaaataa aactattctt
    11341 acaaaacata acttgattga atataacata aatttataac ttattctatt gcccaagcag
    11401 gctataagac atttatagcc tgcttgcccc agttattatt tatattttta tgatacaata
    11461 taagaaaaaa gaggattaaa ttatgagcat aaaaccctgg atagaatatt ttttggattt
    11521 ggcaaaaacc tgcgcatcac gttcaaactg cataagagcg caagtcggtg ctgtaattgt
    11581 aggcgatgat aaaaaaatta aagcaacagg ctacaacgga acgccttcaa aagttcaatc
    11641 ttgttatgaa agaggcgaat gctaccgcat taaaaataat attccttcgg gaacgcgcta
    11701 tgaaacctgc cgctcaatcc atgccgaaca aaatgccata attcaggcag gacaagaccg
    11761 ctgtatggat gcgacaatgt atatctgggg acacgatttt atctgcattc tttgtaaaag
    11821 gtttatagtt cagtcgggaa ttaaagaagt ctatctcaga aaagacgaaa actcaccaat
    11881 cttaaaaatt tctgtcgaag acataagaaa agagcttgag gctgagcctc agttggttta
    11941 aaaagtgaac agtgaactgt gaacggtgaa ctgagactat aggactataa gtttattttg
    12001 cctcgcactt cgcattttta cctatcgagg ataccgaaaa attcgaaaaa ttgtcatttt
    12061 tcgaattttt cggtatcctc atcgcacata tttaacctta acaatgcttt acatttccca
    12121 aaagtgtcta aaatacacgc ttttagctgt gttgttttta taaagatatg taacaattac
    12181 tcctttattc atattacaag aggagttaat gatttgaaaa agtggtataa tattagtaga
    12241 ataattgtac aagtgacaaa tcgatagatt taagcagccc gcaagcgaaa ggttggctaa
    12301 tatatgacag acgtgcaatt tcacagtgat ctggatagat taattgaaat tctgcctaaa
    12361 aaaataacga agcacataac ttatgaaaac atggacgatg taatagaaat agtgctggac
    12421 ataagccgtt taccggaagt ccgacactcg ggaggaagga ttgagtctct tggcgttgag
    12481 ccgataacgg atgaggacat taactatata actcaaaggg tacaggaatt tacttccgat
    12541 aaccgttcag gaatccccgg aacactccac agaataagcg caataagaaa ccgtcaagga
    12601 aaaataatcg gtcttacttg caggataggt cgagtcgtta cggggactat ttcatgtata
    12661 aaggattttg tgactcaagg aaaaagtatt ttattccttg gtcgccctgg ggttggtaaa
    12721 accacaaaat taagagaaat ttcgcgcctt atggcagatg aattgggcaa aagagttatt
    12781 gtagtagata cttcaaacga aattgcaggt gacggagatt atcctcaccc ggcgatagga
    12841 aaagcccgca gaatgcaggt aagacagccg gaataccaaa aagacattat gatagaagct
    12901 gttgaaaacc acacacctga agtaattgta gttgatgaaa tcggaacaga agcagaggct
    12961 caagctgcaa gaaccattgc agagcgcggg gttatgctca ttgcaaccgc acacggaaac
    13021 agcttggaaa atcttattaa aaacccaact ttatcggatt tggtaggctc aatccaatct
    13081 gtaacactgg gagatgatga ggcaaaacgc aggggtagcc aaaaaacagt tctcgaaaga
    13141 gaaaaacagc ccacttttga tatagtaatc gaaataatag acagaaacac tttagcggtt
    13201 tacaaagatg ccgccgaagc cgttgactac attttgcgcg gctggccgat aagacccgaa
    13261 ataagaaaag ttgaccaaaa ttacagcgaa aaaaccactg aaacagtttc aaataaaatt
    13321 tcacagcttg aacaaaaagc ccttccgcac gctgaagaca gcttaaaatt cagtttttca
    13381 agacaaaatt acgtaaaaga agttaaaaat ttcaagaaaa tttatatcta tgcggtttca
    13441 agaagtatag tcgaaaaagt tattgaaaga cttgacctta acgcggaaat tacaagaaac
    13501 gtagaagatg cagatatagt gcttgcccac aagaattttg caaagggcgg ggctaaagtt
    13561 ttaagcacgg caaacgatta cagaatacag gtcttttacg taaaaacaaa ctctatggca
    13621 caaattcaaa aagtgataaa agaggccctt ggaattcaac aggacacttc agaaacgctt
    13681 caaggctact atgacgaagc agaaagagct ttagatgaag ccaaagctgc gatagcaaaa
    13741 atacttgaag gtgaagaaca cattgaactt cagcctcaaa atcagcagat aagaaagctt
    13801 cagcacgaac tggtcgaaca acacaaccta gccagccaaa gtgtgggaga aggcgaacaa
    13861 agacatttga agattgtggg cggcaaggac tttaaagaaa aaaatgcaag ttgaaaataa
    13921 ttagtgtgtg gtgaatagtg aagacgggcg ccggactttc agtccggtgc cgttgttttg
    13981 ttatgtcttt gcgaggagaa acaaagtttc gacgaagcaa tctaaaataa aagaattttt
    14041 ggattgcttc gtcgtgccaa ggcacttctc gcaacgacgc gggtggttga gaattaattg
    14101 gaaaatacgt ttaccttact ccgcttcaaa gagaaaacgc cggctcttaa aaagagccgg
    14161 cgcggagaga atctcgactt tgaagtcttt attgtagaag tttaagagcg attgaaggaa
    14221 gctggttagc ttgagtcagg atacttgctg atgtttgttg cagtatctgg tatttgacta
    14281 agtccgaact tgttgatgca atatcagcat ccaatatcct tgaattggtt tcagacaagt
    14341 tttggttcat aacagtcaag ttgcttgaag ttgctgccaa tctgttaccg tatgcaccta
    14401 agagagctct ttttgagctt aatgaagtta ttgccgtatc aagtctgtcc atataatctc
    14461 tgatatccga agtgctccaa gtttctcctg tagtggaatc cggaattata atacctttag
    14521 gatcatctac gttacctaca gggttaccca agacagatat gtgaacatct gccagtgcag
    14581 ggcctatgtc taaagcgttt gtagcttcat ctgaatttgc accgatttgt aaagacaaag
    14641 atgtttttga gccgtctaaa agtttaaccc cgttaaaggt agtagattct gatatgttgt
    14701 taatgtcttt aagacgttgc ttaatctctt gcaaaatgtt atttttatct gatgccgtat
    14761 aagtttcgtt tgccgcctga acgcacagtt cgcgaatcct ttgaaggtgg tttgtaactg
    14821 taatttcgcc gccttctgca acgtttagaa ggttttgacc gtttgctatg ttagaaatag
    14881 ctttactgtt accgttaagt tgtgttttaa tactttcaga aattgataaa cctgctgcgt
    14941 catcgcttgc gcggttgatt ctcaatcctg aagaaatctg ttccattgct ttttgataag
    15001 cttccgtatt cttagataag ttgttctgag caactaatga agatacgttt gtgttaattg
    15061 ttactgccat gatttttctc tcctattttt taccacaatg caaatatcga ttttatacaa
    15121 atcaaacttt agtatagttt tttgtatttt tttaatacaa aattacctaa aaagcttgct
    15181 atataaagtt tttgcgtgta tcatactaaa gtataaaata tccaccgttt aaagtagaaa
    15241 ggaattgttt tgccgcattt tttcataagc tcagattcaa taacagaagg aattttaacg
    15301 atttctgacg aaaataatta taaacatatc gcaaaatctc tcagagcgaa aatcggcgaa
    15361 aagcttttgt ttattgacga aaaacaaatt caacacgaag ggattgtgca gaaaataact
    15421 tcaaaattga ttgaagttaa aataacaaaa agcgcgaaat ccaatcgtaa attggatttt
    15481 gcgctctacc tcgcacaaag cccccttcga agcgatgctc aaagtctaat tgtagaaaaa
    15541 gcaacagagc ttggagtagc ggggttatat ccgattttaa cggataactg cgcactgaat
    15601 aaagaagtaa taaacaagaa aattccaaaa tggcaaaaga ccatggcaga ggcttcaaag
    15661 cagtgcgaaa gggctgatgt tcccacttgt tttgaacatg caaccataca aactcttttt
    15721 gaaaaaggca gctttgacag gatttttgcg tttgttgaaa gaaaagcgtg ttacaattta
    15781 aaagagtatt tgagaaaaaa cccgataaaa aaaagtgaaa aaattcttgt tataatagga
    15841 cctgaaggcg gattttctca agacgaactt gaatttttta aacaaaacaa aattcctatg
    15901 ctttctttgg gtaatttaat tttaaaagca gaaaccgccg cattcgtggc acttggaaac
    15961 ataatttatg aatacagtaa ttgagaaaat taaaaaagac aaaattataa aaaaaattaa
    16021 aacacattgt aaagctgcgc aagtttacct tgtgggcggc actataagag atttttatct
    16081 cgggttggaa aattttgaca aagacattgt aatagataaa attaacgcaa aagattttgc
    16141 aaaaaatctt gcaaaaagcc ttgacgcaac atttgttccc ttggatgaag aaaataaaat
    16201 ttacaggctt gttttaaaag ataaaattaa ttacatagac attgcaaata ttatcggaaa
    16261 ctctattgaa gacgatttaa aacgcagaga tttgacgata aattctgttg caataaattt
    16321 aaaaacattt gaaataattg atattacagg cggcgttaag gatttagaaa ataaaaaaat
    16381 taaacatata tcagagcaga attttgttga tgatccccta agattgcttc gcgcctatag
    16441 atttggggct gttttaggat ttaaaattga tgacgggctt caaaaaatta tcacacgcca
    16501 tgctgccaaa atacaaaagc ctgccgttga gcgtataaat tatgaacttt taaagctttt
    16561 tgaaggaaga tttagcagcc aaattctttt tgaaatggat aaatcggggc ttttggaaga
    16621 aattttacct ataataaaag atttaaagaa agttccgccc aattctcacc accatttgaa
    16681 cctttttgac cactcgatag aaacggtaaa acaaatccaa agaatttacg aagaaagttc
    16741 aaatgaggtt aaattacatc ttgaaaaaca cgactttggc ggagcttcaa gacttgctca
    16801 tttaaaattt gcggggtttt tgcacgatat aggaaaattt gaaacctgga caatcgaaga
    16861 ggaaacgggc agacacaggt ttattaagca cgacgattta ggctcaaaaa tggctattga
    16921 atttttgaaa aaaaataagt tttccaaaaa acagattgac tatatttcaa agatgattaa
    16981 actgcatata tatccttctc atgtgattaa aaaccctgaa ataaacgaaa aactttacat
    17041 gcgcttaatc agaaaaatga aagagaatgt gattgatatt attattcttg cgatggcaga
    17101 caggtactct gcaagaggcg ttgaaataac agatgaaata gtaaacaaaa atatatcgga
    17161 tttaaatttg cttttggagt tttatttaaa aataaaagac gagcttaaac ctttaccgaa
    17221 attgctctca ggtgatgaga taatggaaat tttaaacata aaaccttcaa aagaactggg
    17281 tgaaattatc aaaaagcttc acgaatccca gctttcaggc gaaattcaaa caaaagaaga
    17341 agcgctaaat tttattcaaa attttaccgc caagaacact taaaaattct tctgccgatt
    17401 tttgggggga tttagcattt attattgctc ttacaacagc aattcttttt gcgccgacac
    17461 ctagaacctt atcaacattt gccccgtcaa ttcctccgat tgcaaagaaa ggaatttcaa
    17521 tattttcagc cgcccattta acataatcaa gcccgacggc ttttctgcct tgctttgtgg
    17581 gggtttcaaa aacaggcccg acaccgatat agtccgcgcc ctcttcaact gctttaaggg
    17641 cctgctcggg cgcatgggtg gacacaccta taatcatacc ttcccctatc aacgctcttg
    17701 ccgattttac atccaaatca tcttgaccca aatgtacccc gtcagcttcc aaaatcattg
    17761 caatatcaac cctgtcattt acaataaaaa ttgcaccata ctgcgcgcaa agctgtttaa
    17821 cttttttgcc cagctctatt aggttttttg cgctcattgt tttttctctc agctgaacaa
    17881 tctcaactcc gccttcaagt gctgatgcaa ccgcatccaa aaattcgtct tgcgtttcaa
    17941 atttgtccga atttgtcacc agatacaaac gtttatccga aagcttaatc gaactaaaat
    18001 tttcctttaa tttttcccac ataatttttt ccaaagtgta ggatgtatat cttaattttt
    18061 caaaaacttt tacgttatca gagctttgtc cggaatattc cgcaagggtt cttaatgcct
    18121 gctgaagcct ttttatattt gccttaaaaa tattttcaac atcggttctt ttggtgggat
    18181 ttgaaatatc aaccccgacg tcattttgag tgtctcttga tttaaggcat ttgtcgtaat
    18241 cttcttgctg aaaagcacaa attttatgtc ttatgttttt taatttttcg gacaagtcga
    18301 aatcatcgag caaaaaacgt gcaatttctt ccaaaatcct tgaagcttct gcggctctgt
    18361 ttaaatttgc atcgattatt cttttcaata taatcttcct tttaataaat cccctaactg
    18421 attatataac aaagagtgag caaaattcca caagatttaa ttaaaatttt taaaaatacc
    18481 tttgagtctt tgtaaaaaag tttaaccaga aaggaaagtg atgcttggga aattgcctta
    18541 taaaaaaatt cctatggaag aacttgttat gctttcccaa aaaagcgatt acagggcact
    18601 tgaagaatta atcaaacgtg aacaaaaaaa tatttttgca gctttttcct atctcagcaa
    18661 aaaaagagaa aacattgcag atttaaccca agaagctctt ttgagaatgg caaggggaat
    18721 ttcaaatctt aaaaacccca aaacctttaa aagttggcta aatcaaatcg taacaaacct
    18781 tttttacgac gacctgcgaa aatcaacccg cagacccgaa accatttcaa tagacaacga
    18841 tgaaaacgaa aacccatatc acacacaaat cccggataca aagcatcgtc ctatggaaaa
    18901 aattatgtca tcggaattag acagacttat aaaagaagaa atacaaaatc tgcccgacca
    18961 tttcagaatt gcgattatcc taagagaact tcagggcttg acttacgaag aaatagcaaa
    19021 cgcaacaaac accaatgtcg gaacagttaa atcaagaatc gcaagggcaa gagaaaaact
    19081 acaagacgga ctcaaacatt atatataaag gagtaaacta tgactataaa cctcacctgt
    19141 acacaggtaa gcgcgctttt atctttttat cttgacgaca gacttaacca gcagcttaaa
    19201 cagtttgtca aagctcacct tgatatctgc ccgacgtgca gggcaaaatt tgaaactctt
    19261 aaaaaaatgg tagaaagctt aaaagaagtc catgagaaag tagcgacagt agaatctcag
    19321 gaaaaagaag cttcagaatc acctcaatac aacgaattca gaacaaattt atccgcatac
    19381 atagacaatg aactttcaga tgaagaaaat atcaaaatta aaaaatatgt aatttcaaac
    19441 cctaaagcaa ggcttgaaat agaaaatctc tatgaatttg aaaaaactct tcacaattct
    19501 tttgaaagaa taaaaaacca ctcaaaaaca gacttttcaa agcaaattat gagaagaata
    19561 gatattcaag aagaaattaa cacccctgat tcttttgcaa aaataatcgc gattttcatc
    19621 attctttttg cggtttgtac tttagggatg ctttttattt actggatttg attacaacaa
    19681 catttcttca aaattcacag tatcgttgta gctgaacatg tggataaatg tataaagtgc
    19741 ttctgcagag aactttcttg taccaagctg aagcataatt cgaagtgctt cttttgtatt
    19801 ttttatatta tattcggttc tgtttgaagt tatattgtca aaacagttac aaacatttac
    19861 tatttgagcc tcaacattta tgctgtcccc tgattttcct ataggatacc ccgaaccgtc
    19921 attgttttca tggtgttcaa gtgccactct ggcaatattt tcgggtaaat ccatttcatg
    19981 tttaattatt ttataaccta ttctggtatg attttgaata gctttgtgct cataacttga
    20041 aagattgctt tttaaaagca aatcttgagg aatttttgtt tttcctatgt cgtgcaaaag
    20101 ccctgcaagc actacatcat ttatcaaatc ctcattaagt cccatttttt ttgccactgc
    20161 tccggataga atcgcagtat taagagcatg gcattttttg tattcaccca ataatttaag
    20221 ctgggaaaac aaaaatacat cgttattttc aaatataata tcctgaatta ttttgtcacg
    20281 aacatttgca aacattccaa gcatttccaa cggaggcttt tcttccatca tttcaagaat
    20341 attgttgtaa aacgccttaa tcttaatctg ggtttgaggg tctatttttg cacgcttatt
    20401 aacaggtcct ttgtaactca taatatctat gtcatcaaca gaaagttttg tttttgtaat
    20461 tttacttgaa ggattcatgt caaaaacttc gacaacaacc tttggttttt ccggcttaac
    20521 ctctttttct acaggctttg cctcaacaac tctggcttct gcaggaacag cctgaatttc
    20581 atcgtctctg taaagaacag tcatatgcct taattgcagg agtttcccgg gagttaaaac
    20641 ctctccggca gaaaataatt tttcgccaaa ctctgaataa atattgaatg gcaatatttt
    20701 ataatttatt atctcttctg ttgaaaccag tttcatattc tatccttcac aaatattata
    20761 gcagattttc aatacaaaaa actatacgtt tagatgaaat tttacattgt ggtagaaaca
    20821 ctttttgtat tcattgtagt tattttctct tttatatttt tctttcggta ctttttggtg
    20881 gcaaaaagta ccgcaaaaag ccacgacacg gatcttcgtt cgctaactat tactaaactc
    20941 aacccaagct cgcgaactcg tgccttcggc actcaaacac actcgctttg ccgttgtttc
    21001 gttaagtaat aattgctcac tccgatacgg tcgggggtaa atgcttaagg gggttcgggg
    21061 cgtagccctg acgctttggc attttgttcg cggttttttt cttttcttgc cttcttttgc
    21121 ctgaaacttg tttcagtgca aaatggcgtc acgaagagtg actaccgtct ggtttctttt
    21181 ctttttgcct acttttgccg aaatctgtga tttcgtgcaa aatggcctca cgttgtgaga
    21241 aatcgcgaca aaaaaagaaa agaaatgaga caaaataaga ggtagccagg taaaaaacat
    21301 aaaaaaaaca atattttaat gggctttatt tatatacatt gccaaaatat tattaattta
    21361 ctattttaat tattttgcgc aagcgtatcc tgacgaataa attcacaaat ttcttcaagc
    21421 tttttgtctt ccattgcctc aattaaatca tcaactgttt ttattgtttt aagctcaaat
    21481 cctaattccg cataaagcac gcaatcgtta caaagcctgt aatccccgta ttggattgaa
    21541 ccagcaagac acttgaactt tccgcaacaa tcgcagtcaa aaaaatcatt tacaatatca
    21601 ggattttctt gaatatcgtc aagccgcatt tgctcgacta cctcctgcat ttgttttaca
    21661 attttttctc ttgatttcat ttattcaacc aactctttca tttctttgac tgctttttct
    21721 aaccccacat aaatcgccct tgaaataatg ctgtgtccta tattcaactc aacaaggttt
    21781 gggatttcct tcattctgtg aacatttttg taattaagcc cgtgacctgc attaactttt
    21841 aaccccaaag attgcgctaa tgaggcggaa tttttaagtt ttaaaaactc ttcttcctca
    21901 ttctctcttt cgaaggcttc agaataagcc cccgtgtgga gttcgataaa ttccgctcct
    21961 gtttcaaagg ctgcttgaac ctgtttttcg tcggggttaa taaaaaggct tacggcaatt
    22021 cctgcatcat ttaaaggttt tacgaatttt tttgcgcgct caagttggga aaaaacgtcc
    22081 aacccgcctt ctgttgtaac ttcctgtctt ttttccggca ctatacagca ggcggaaggt
    22141 tttaattcaa gcgctatttt ttgcatctcg tctgtcatcg ccatttcaag gttcatgcgt
    22201 gttttcaaaa cttttttcaa cgcccgaaca tctgcgtctt ttatatgtct gcggtcttct
    22261 cgcaaatgta cagtaatgcc gtcaactccg cacttttcac aaataagcgc acattctatc
    22321 aaatccggtt caaaaccgcc tcttgcgttt ctcagcgttg ctatgtgatc aatatttatt
    22381 cctaaaagca ttattttacc tcgattttat ctaattcctg tttaacggct ttgggcgaaa
    22441 atgaaagcgt tttatactgt ttaagagcat gtttaagagc tttgacataa tcctcgttgt
    22501 ttccctgaag cttgtatatt tgagccaaaa cataatataa atcagcttta tcaggattct
    22561 ccgttatcgc atgctcaatc aatttttgcg cctcttgtat ttcaccggat ttaatcaaaa
    22621 tttttgaata tattttatag gcttcaatgt ctaaaggatt aagctcaagg gttttttgca
    22681 aattttcgac tgcaagctcc atttcgcctt cttcaaaggc aatagaagca atattaaaat
    22741 atgcccaact gtattccggg cagacttcaa taactttgta gtatttttct tttgcctctt
    22801 cggtttcccc tttgttttta tgaatgtatc ccaaataaaa gtatgcaaaa acatcgcaag
    22861 gattgtattc aagtgctttg ttaaaacatt ttgccgcagg ttcgtatttg ccctgtttaa
    22921 aaaaacaaag cccaagatta aaatagctgt ttgaatcgtc tttgtctttt gtaaggactt
    22981 ttttgaagca ctcgattgcc ttatcgtatt cctgcttgtc aatgtaaaca agccccaaat
    23041 tgtagtaaaa atcagtttca tcgggcgata tttctgttgc ttttttatag taatgtattg
    23101 cattatcaag tttttttaat ttttcaaaaa caattcccaa attgtaaaaa agccccgcat
    23161 ctgtggggaa aactcccgac aagaacaaaa actgtttaag tgcttcctcg ttaaatccgt
    23221 tgtcaagcag caaaactgca agcctacgcc ttgcctgata atttgacgaa tcagacttta
    23281 aaatttcctg taattcttct atttcttcaa cagcagacat atttccccaa aaaatcaata
    23341 taacaagtta agtataacat gtttttaaaa atgtgtcatt ctgttaaatc aaaagacaat
    23401 aactggaata tgcttataaa agagattctt cgctacggac taaaatgaaa aataaacctt
    23461 taatatgctc agccttttag ccaaatttcg ctaattaata atttggctta ataatttaaa
    23521 tatagcgcaa acgcaataat aataataata ataataataa taataataat aataataatg
    23581 taaaaaagta gcccgaaact ccttttttac attattattg tgctattatt taattaataa
    23641 attaatttga ggatttttta ctgagtgata aaatttacta aaaatacaaa agtgtcaaca
    23701 atgtctcagg gttatacagg ctttactctt gcagaggttt tgataacttt gacgataatc
    23761 ggtgtggttg cagcacttac ggtgccgaat ttatatgcgt attatcaaaa aacacaatat
    23821 gtaacgcaac ttaaaaaagt ttattcagag atttcgcaag caataaaact tttaatggca
    23881 gatgagggag ttaataaatt gtcagcttca aatactttga cttttgatga agtatctgat
    23941 tatgacgagt ttattcaagg attaggggat aatttcttta aaaaatactt caaggtaatc
    24001 aaagattgcg gaactgggta tgaatgtttt gcgaataatt ataagacaat tgatgaaaat
    24061 aatttcagtg gcatcacttg cagttatagt gtcattattg caagcggtgc aagtatttgc
    24121 actagtcaga ttgcatctcc ttctatgccg ggagattttt ttgttgatat aaacggacta
    24181 aatccaccaa atgtagcagg cagagatctt tttagttttt cgtattacca tgatgggtct
    24241 atagattcat tcgtttctcc agaatgtaga aaaggagtac ctaattcttc aggattactt
    24301 tgttttggtc ataactctcc aaaagaggct cgagactatt tttttgataa tggagagggc
    24361 acgggttgct taaaaagcac ttacggggtc ggctgttttg ctaagattct aaatgataac
    24421 tggaagatgg attactaaac acgtcattgc aagggggtaa atgcattttc gctttttgta
    24481 ccacactcac gtcattgcga gacatgagtt tgcaaaactt tactataatt gaaggaacga
    24541 agcaatctca ttcgcatcct caagacttgc aagagattgc ttcggtggat aataattgtc
    24601 aaagccgata actctcgtct cgcaaagacg tgaacggata gacaaaaagt taaaatctca
    24661 tttacccttc gcaaagacgt gaagttagtt tataggatga atggataaag ggtgagtagc
    24721 taaaaagcta aaagactata agccaagtcc acttctcaca gttaaccttt ctgcatattt
    24781 atttgcaaaa gtaagaattt aagctaaaat aaatttatgg aaaaggttaa accgattgtt
    24841 ttttatctga tttttttctt gttcattctt gcctattgca caggcgcagg cggctacgat
    24901 tacgaccttt gggcaagact tattgcagga atgggctttg tacagggcgg gcatgtctta
    24961 aaacaagatt ttttgtctta taccccaacc cacacttggt ttgatcatga gtggggcagc
    25021 ggagttatat tttatcttgt ccaacacttt ttccctacag cagggcttat tgttttgaat
    25081 gccgttttaa tatttatcct gttttttata attacaaaaa tcgtaaattt aagaggggta
    25141 aaagttacaa cagcatataa ttttatattt tacctttttg cttttcttgc aatagctata
    25201 aatgttatgg agcctataag gtgtcagatg ttttcgttca tattttttgc gctgttcatt
    25261 tatattctgg agcttgcaag aaaggggcaa aacagaccgc tttggctttt accgtttatt
    25321 atgataatct ggaacaacct tcatggcggc tgcgtttctg gtatcggttt aatttgtctc
    25381 tatattttgg gtgaattttt aaacaaaaaa atgtttaaaa aatacatttt tacacttatt
    25441 ccgacaattt tggttttggt aattaatccc tggggctttt catatcttaa ttttttaatc
    25501 gatgctgtta caatgaaaag acctgatatt atggaatggt gggggctttt tcacaaaatc
    25561 cacatccaca attttttaat ttttaaactg ttttctctta ccatgatttt gacttatctt
    25621 atttcgtctg taaaaaattt ttcttacaaa aatatcgaca aaacaaaatt tctaatactt
    25681 tctgtaactt tatatcaggc aataattcac ataaaaatga ttccgttttt tgtaatttct
    25741 gccacttgtt ttttgtatga tgatttttac acgcttttag acaatattat taaatttata
    25801 aaacaaaaat taaaaattcc tgcggaattt ttcggagaaa aatttatagt aaaaaaagaa
    25861 tttgcaattt actgttttat cataatttgt tcaatcgcaa caatacacac aaataattta
    25921 ggaataattt taaaaagcga caaatttgct tacaaagaaa ttgaattttt aaaaataaat
    25981 aatataaaag gaaatttgtt cttaaatttc gggcagggaa gctatgctgc ctacaagctt
    26041 tatccgaaca taaaaatctt tatggacggg cgctacgagg aggtttatta caattacatg
    26101 cttcctgtta tgaacgaatt ttattattcg aaagtcgata atacaaagct tatgaaaaaa
    26161 tttccgcccg atattatgat aatcgaaaaa aattatccgg tttataaaaa ccttttaaaa
    26221 cacaaaaatt ggaaactcgc ctatgaagga atattttatg gagtgttttt aaataaaaaa
    26281 gatttaaaga aaaattataa acttccttca aatgatagaa aatattataa aaaaacactt
    26341 tttgacacaa atatcaattt caaaaaataa aaacttcttt tatggtaaaa tcaagttatg
    26401 agcagagaga ataattcttt aaaatacact tgtatattcg gcggcggtgc cataagaggc
    26461 gtttcttacg tcggtacaat aagggcgttg gaagaaatag gaatttgcgc cgacacgata
    26521 gcaggctcat ctgtcggggc tatttttgca gggctttggg caacaggata taaagccgat
    26581 gaacttaaag atattttcat gcacacaaat tatgaacttt tcagagatat tcacttcggg
    26641 tttggaaaag attttgccat aagcaaaggt gaaatttttc ttgactggct gcgtgattta
    26701 attgagcaga aattctacgg cgaacgctac aaaaaaggcg aaaatcctcc cgtaacgttt
    26761 aaagatttag ataaaaacgt ggttataatt acaacggatt tgaccaattt taaatataaa
    26821 gaattttcca aattcgaaac acccgatgtt gaaatcgcct atgcaatcag gatttcttct
    26881 acaatgccgg gtttaatgaa gccaattgtc tatcaagaca gtcttttagt tgacggcgac
    26941 cttcaaaaaa gctggccttt atggagattg tcaaaaaatc tttgtcccga tgatcaaagg
    27001 gttttggaat tcagactgga aggcgattat gaaggcaaag gaagcaacgc gataaatttt
    27061 attaacacaa tttacagctg cgtaacctcg cttgcgacag attttatcat tgatacctac
    27121 ggaaacaaag acaaatttga ttatataaaa gtaaacacag gtgctgttgt ggttgtggat
    27181 ttcaaccagc ccaaagaaaa acgcgaagaa ctcatggata taggctacaa acagactgtt
    27241 gactattttg aagttgattt gagaaagaaa aaaagagaaa ttttaaaaca atatgaaaga
    27301 atcttaaaac acgtaaaaaa aatcaaaaat gatgttgaat caaataaaat aaagcaggca
    27361 caaagtgggt tgggactttt gtatatggat ttgagctcaa ttaaaaaagt aatagattta
    27421 aaatatttta atttgattga tgattttaaa gagtgtttta tgtcgaatgt caaccctgct
    27481 tcgctttttg gaatattttc tcttaacaat tcaaaacttg taaaatcaaa aatcaacgcg
    27541 ataaattttg agctggaaga aaaagtggca gagcttgaaa gctatgtttt taaaatatag
    27601 gggattaaga tgcaggaatt aaaaatatac aaagacaaag acattgacga aaaaattata
    27661 aaagacaaaa aaataacaat tatcggctac ggctcacaag gctacggcca gtcgcaaaat
    27721 ttgagggatt ttggctgtaa tgtcattttg ggattaagaa aagacggggt ttcttataaa
    27781 aaagctcttg ccgaaaaatt tgagataatg gaagttgaag acgccgtaaa atgggcggat
    27841 ataatacaaa ttcttgtacc cgatgaaatt caggcaaaac tttataaaga aaaaatcgca
    27901 ccttatctca ggacggggca atacttgatg ttctcgcacg ggtttaacgt tcatttcgga
    27961 aaaattgttg caccaaagga tgtaaatgtg attatggtag caccaaaaag cccggggcac
    28021 gcacttagaa gtgaattttt aaaggaaaaa ggtgttcctg ccttggttgc tgtttatcaa
    28081 gattattcgg gtgattcaaa agaaatggca cttgcctacg ctaaagcaat aggctcgggc
    28141 agggcgggaa ttattgaaac cacttttaga gaagaaaccg aaacagacct tttcggcgaa
    28201 caggcggttt tgtgcggtgg gtgttccgcg cttatcaaag caggatttga aacacttgtc
    28261 gaagcaggtt attctcctta tatggcttac tttgaagtgc ttcatgaact aaaacttatc
    28321 gttgatttaa ttaatcaggg cggcttaaca gatatgcact attcgatttc aaacacagca
    28381 gaatacggtg atcttacaag aggaccgaga gtcattaacg acaatacaaa aaccgaaatg
    28441 aaaaaaattc tcaaagaaat ccaaaacggt acttttgcaa aagaatttat tgacgagata
    28501 aattcgggaa gtaaaaattt cgacaaattg agaaaacaaa tcgaagaaca ccagatagaa
    28561 aaggtcggaa aaacaatccg cacaaacttt tcatggggca gtgaaaacaa aattattgat
    28621 agagaaaaga actgaggcag aaaatgtttg acaatgaaac aatatatgaa gtggtaatat
    28681 tttcaatcgg agatacaaaa tccggtacaa aaatgggcaa acttcagctt aaaaataccc
    28741 aaaatgaaac ccttttaaat tgcattttgt gggaagaaac ccttaaccgt attgacagca
    28801 aacttttcag gacaggaaat ttggtaaaaa ttgtctctgc cacttataac gaaaagttca
    28861 acaactgcct tgtatcggca cttgagcttg taaaagaagc aaaactcggc attgacgaga
    28921 gcgaaagaaa ttctctttac agtgaaattt tagaatatgt aaacaaaatt tctgacgaaa
    28981 agcttaaaaa ttttattatt gattatttta aagagcatga aaaagaaatt aaaatagcac
    29041 ctgccgcaaa acttatgcac cacaactata tgggcggatt gcttgttcac atagtcgaat
    29101 gtcttaagtt tgccgatgct aatatggaac tgtttaaaat caaaatcgat aagaataaaa
    29161 tttatgctgc ttgcatcttg cacgacatag gaaaaatatt tgaatacaaa atcgacaccg
    29221 aaaccggaat tatagattac gacgaaaatt tcaggcacga ctgggtaacc cactctcaat
    29281 acggattttc aatctgcatg gcacaaggct ttaaagatgt tgcaagaatg attgcagcac
    29341 accatgcaag aagcgattgg ggagcgatta tagaccttga ccaaaaagat ttggaatcgt
    29401 gggtttattt aatgcatcac attgacgacc tttctgcaaa atacggcaga ataagtgttt
    29461 cgcttttaag ctgaaaaatc tgacatagcg gttgacaatt tgttgaaaat aaattattct
    29521 aagaaagtag ccaagaaaag gtaaagttat gcataaaagg tggtacgatg ccgaccccac
    29581 agtgagtttg gcaataagtt tattaagaaa cacaaatgaa aaaacgcagc tcaggtgtgc
    29641 cgattttata attgactatg caaaacaaag aaatgttacc cgacccgcaa acgccttaaa
    29701 tgatgctttt aactacattt tgagacgttg gtacgatcag gataaaaaaa tagcagaagc
    29761 ttttgaattt ttcgaagcag ctcagcctga tgtccaaaaa gaaatttcaa tagagataat
    29821 taactttttg cagttttctg aattatatta agccttttcg ccttttttga ttaaatattt
    29881 tatataaaaa ttagtgaaaa attttcttat tttgccgtaa agtcttactc tgctcatttt
    29941 gagaagtttt ccgtcgccgc ataaaaccgt tatttgattg tttttaggcg attttgcaac
    30001 gattgaaccg acttcgttaa aatttgtatt gttttccaaa atttccaaac aataagggtt
    30061 tggtacaaaa aactgatttt tatactcgta ataacattta tgccatgggt gaaatgccct
    30121 tatttgcgcg cttagttctt ctgcagattt tgaaaaatcg agcataactt catcttctgt
    30181 gataacggaa aaataacttg cttttttctc atcctgaaca acaggaataa ttatttcttc
    30241 gcctaattcc ttaatcaact ctgtaaccgc ctgccttgtt gcccctgcaa tcttttcttt
    30301 gagttctttt cctgtgtctt tgtctgttat ttcaaccgtt ttttgaagca aaatcggacc
    30361 tgtgtcaaaa tctttatcca ttaaatgaaa cgtaactccg gattgttgct caagatgttt
    30421 tatggcttgc agataaggat ttggtcctct gtattttggc aaaagcgaag gatgaacgtt
    30481 tattgtagca atttttggca aatcaataat gctctttttt aacttttcgc cccatgtagc
    30541 aacaaaaaca atgtcagggt tgagttttag gatttctttt ttaaactcga cactatttgc
    30601 actttttgcc ttaatttcat aaagcttatg accttttata taattgaatt cttttgacgg
    30661 gttaaaaata tccagaatca aaccgtctaa ccccttgtgg cggactttgt cgtatctgaa
    30721 aacacctacc acatcgcagt gcgcatctaa gcaccctgca ataaggttca taaacatttc
    30781 tccataacct agaataacaa cctttggcat aaagaaatta tagcataagg ggcacaaaaa
    30841 tttgtaaaat ttttgtgccc cttatttaaa acttaattaa acctctgtaa cgactttttg
    30901 cagacctttc ggtttgtcca cattgcgccc cagtttttgt gcaattttaa gcgcaagaag
    30961 ctgtaccatt accgccattg aaaatatctt taaattttca tcatcgactt ccaaatcaac
    31021 gttaaaagca gaattaatcc tgtcacttga tgttcctata acgcaaatcg gcggattata
    31081 atctcttttg attttttcca tgtgtttaat tgtcgaataa gaaatcggtt tgttggaaac
    31141 ataaaccagc acgcatttgt tgtttaaaac ggcaacatgc ccgtgcataa attcacccaa
    31201 aatatgcgac attacgttaa tataagaggt ttctttaatt tttaagcatg cttcttttgc
    31261 aagcgcataa gaaattccgt ctgcggttat caaaatgttg ctgtattttg acaaaaacct
    31321 tgcaagtgct ttaatttgag ggcttaattc aagagttttt tccaaaattt caggtaaagt
    31381 cttaactttt tctaactgct cggttatatc aacttcgtga atttgagcgt atttaagtgc
    31441 cattaaataa agacaaagca tttgtgcggt caaggatttt gttgaagcga tacttttttc
    31501 ttcacccgcg taacagttaa ttctgtaatc tgcagtctgc catgcggttg aattttcttt
    31561 gttggtaatg cacatggttt tcacacccag ctgttttgct ttgtttaggg cactcaacgt
    31621 atctgttgtt tcacccgatt gggttataaa aatgtataaa atatttttat cgtgtggaac
    31681 tatttttttg agcaaaaatt cacttgaata aatggctctt gcttctatat cggcaatttc
    31741 gccaaacatt tctgcggcaa gccttgcgca gtgataagaa gttccgcttg caacaaggac
    31801 tattttggta attttagtgg gtatatttaa aataatataa ccgtttgggg caatatattt
    31861 ttccaaaatt tgccttaaaa tttcgggctg ttcaaaaatt tcggcttcca tgctggtatc
    31921 aaatttctgc tgttttaaat ctgccatgta catctccttc acaaaattgt gaaattaaag
    31981 ctaaactcaa ttaataaata atttgacggg actatcggta ttcaatcata aaagtttaat
    32041 aaatttagtc aaatattgca aattgttaac tttcaagtaa acttttaatt aattcattaa
    32101 ccgttttcgg gttagccctg ccttgtgttt ccttcataac ctgtcccaca aaaaagccca
    32161 aaagttgaac ctttcctgcc ttgtatgcac taacctgctc gggatttgct tctataattt
    32221 ttttgactat ttccttaatc gccccttcat ctgaaatttg gcttagccct tgtttttcaa
    32281 taatctcgga agcttttgtc ccttctttta gcatttgcgg aataatttgt tttcctatgt
    32341 tattggaaat tgtgcctttt tcaatcagat tgaccaattc ggcaaggttt tgcggtgtaa
    32401 gttttgtttg attgatgtta attttttctt ctttaagcag agctgcaata tctcccatta
    32461 agaagtttac cgcagtttta acatttgcgc cgagttttat cacctcatca aagaaaagtg
    32521 ctgtttccat ttcctcaaca atgactttag catcatactc acttaatccc agtcccatgt
    32581 agcgttctat tttggcactt ggaagctcgg gcatttttgc tcttatttct tctacccact
    32641 ctttcgaaat ttcaagaggc atcaaatcag gctcgggaaa atacctgtaa tcgtgggcgt
    32701 cttctttgcc tctcattgat tttgtttcgc ctgtgttgtc attccaaagg cgtgtttcct
    32761 gaataacttt ttcgccgctt tctactatat caatttgtct ttcgatttca aattcaatcg
    32821 ctctttgaag tgccctgaag ctgtttacgt ttttgatttc cgctcttgtg ccgaattctt
    32881 ttgagtcttt gggcataaca gaaatattgg catcgcagcg catggagcct tcttcaaggt
    32941 ttccatcaca aacgcctata tatcttaaaa tatttcttaa atgttcgaca taagcctttg
    33001 cctcgtcgct tgaacgcata tcaggctctg atacaatctc taaaagaggc gttcctgctc
    33061 tgttcaggtc aacaagcgaa tagcttgaac catggattcc gtctgctccg gcatgtacga
    33121 gtttgcccgc gtcttcttca aggtgagccc ttgttatacc tattcttttg ccgtcgattt
    33181 caacagaacc gtttacgcaa ataggctcat cgtattgaga tatctgataa cctttgggaa
    33241 ggtccgggta gaaatactgt ttacggtcaa atttgcagcg gttgggaatt tgacagttaa
    33301 gcgcaagccc tgttaatatt gccatattta cgacttcttt gttaagaacg ggtaaaacac
    33361 ccggcatgcc taaagttata ggacttatct gggtattttg ttcgtttccg aattctgttc
    33421 cgtcgggtgc gaaaatcttt gacttggttt taagctgtgc gtgcacttct aaaccaatta
    33481 caacctcgta tttatctttt aattcctgct ccatttaaaa atcccttctt agattatttt
    33541 tatattaatt ttatcacaaa ataatttatt catttatttt cctgatataa taatcctatg
    33601 actgaagtta aaaaaataaa acttaaaaat ttcgaaatcg gcggtaataa attaactatt
    33661 atcgcaggtc cttgcgttat tgaatctatg gatattttaa ataaaaccgc agaaggtctt
    33721 aaaaaaatta cagaagtttt ggatgtgaat tttattttca aatcttcttt tgataaagca
    33781 aatcgctctt caataaattc tttcagaggt ccgggcattg aaaaaggact tgaaatgctc
    33841 tccaagatca aaaaagaatt tgacctgccc attgtaactg atgttcacac agaatcagag
    33901 acaaaaatcg cagccgaggt tgccgatata atccaaattc cggcatttct ttgccgtcag
    33961 acggatttgt tgattgcagc tgccaaaaca ggcaaaattg ttaacattaa aaaaggtcag
    34021 tttttagcac ccgagcagat gaaatctttg attaaaaaag ttaccgattc gagcaatgcc
    34081 gacgttttgc tcaccgagcg cggtacaacc ttcggataca acaacttggt gtctgatttt
    34141 cgctcaattc ctattatgca agaattcggt tatcccgtaa tttttgatgc gacacacagt
    34201 gttcagcttc ccggtgtcgg cgaggaatgt tcgggcggcg acagaaggtt tgttcccgta
    34261 cttgcaaaag ctgcggttgc agcaggagct gacggattgt tttttgaagt tcatccggac
    34321 cctgacaagg cactttgtga cggaccgaat atgattgcac ttaaagatgc gcaaaaactt
    34381 tttaaaactt gcagggatat ttttgagata gtgaaataat gattttaaaa cattttacag
    34441 tcgggttttt agaagtaaat aattatctgc ttattgatga aaaatcaaaa gaagcgattc
    34501 taatagactg cacagataaa aatccgcaaa ttgaagaagc aatcagagaa cttggcgcaa
    34561 aattaaaata tattttgctc acccacgggc attttgacca tgttttgggg gtaaacgagt
    34621 ttaaaaagtt atacggatgc aaagttttaa tacacaaaaa tgatgaaatc atgcttgaaa
    34681 atgtcgataa aatttcttca agttttggct ttggtaaatc tgaggcgcag cacacagacg
    34741 gttatgtgga agataatcag agtatatatt tcggcgggca tgaaataaaa gttatacaca
    34801 ctccggggca ctctcaagga agcgtttgct accttattga cgacatgctt ttttcggggg
    34861 atactttatt taaagaatgt gtgggaagaa cagaccttta cggcggtaac tttgaccaac
    34921 tcaaagcaaa cattgaaaac aaacttttca cccttgatgg tcacacaaaa gtttttcccg
    34981 gccacggcga attcagcaca atcgaatatg aacgggtaaa taacgaattt ctataaaaag
    35041 caaaacgtga ataggtaaat aagtaaataa gttcaatttg ttgctttgca atataaaaca
    35101 aaaaaaatta aagtagggtg ggttccctaa cccaccgaaa cgaggaaaaa tgaaagagac
    35161 tttagaaaca attttaaaaa atgcgaaatc agatattcaa aaagcttcga caaccgaaga
    35221 cattgaagaa atcaagaata aatatctgag cagaaaaggc gagcttaatt cgattaaaaa
    35281 aaatcttaaa gacttgtctg atgatgataa gagaattatc ggtgctttag caaacgatat
    35341 agcacaagag ttagaaagtg ctttggatga aaaattcaat acaatttaca gaaaagaact
    35401 tgaacagcag cttaaaaaag aacaaatcga cataactttg cccggcaatt ttacccctaa
    35461 aggcaatgtt catccgctga cttccaccac aaacgaaatt gtttcaatat tgcagacttt
    35521 aggcttttct cttgttcaaa gtgaaaattc tcctgaagtc gaaaccgaat atttcaattt
    35581 tgatatgctg aattttccgc ccgatcaccc tgcgcgcgac atgcaggata ctttttacac
    35641 aaaaatcgct tcaaatgtta ttttaagaag ccagacttca aacgcgcaag tcagagaaat
    35701 gactgtaaaa aagcctccat tgagggtaat ctcaccgggc agagtttaca gaagcgaagc
    35761 agttaatgcc aggaaaaaca atcttttcca ccagatagaa gggctttatg tcgataaaga
    35821 cataactttt gccgatttaa aaggggtttt aaacgaattt ataagacttt acttcggctc
    35881 aagccgtgta acaagattca gaagcagctt tttcccattt acagaaccca gtgcagaaat
    35941 tgatgttcag tgcataatct gcggcggcaa aggctgcaaa acctgctcta acacaggttg
    36001 gcttgaaatt ttaggtgcag gaatggtgga tgtaaatgtg cttaaaaatg tcggtatcga
    36061 ccctgaaatt tatacgggat ttgccttcgg aatgggtgtt gaaagacttg caatgcttaa
    36121 atatgcaatc gatgatataa gactattttt caataatgac gaacgcttct taaaacagtt
    36181 ttaacggtat tattgcccta atcacatttt tatgatatga ttttttcatt aaaaaaannn
    36241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac ggtattattg ccctaatcac
    36361 atttttatga tatgattttt tcattaaaaa aagagagggt tatatatggc ttcaattata
    36421 gattcattta gagaaacatt taccgataac atgtcttttt tcaaactgtt aacctttgct
    36481 gttccgcttt attttgctca cgatttatat cttaaaaatc cgcaaaatcc cacgggtgca
    36541 ctttggcttg cggggataac aatattcttt ctgtttggat ttttaattaa agtaaccggc
    36601 gaggttctaa acgaaggtga tacagtttta cctttcttaa accctttaaa actggctctt
    36661 gcctcactta aaggaattat tgctataggc cctataacct ggctttctgc atcaattgca
    36721 acctacgtta ttcccaaagt taatgttata ggttggttca atataacttt tcaaattgtt
    36781 atttggctaa ttgttatagg aattgctctt acttcttttt taatgtttgt aaaaaatgaa
    36841 aaaatagcag actcttataa tttaaaagtt ttattcaata aatctgtcga tttgagcata
    36901 gccataatat tttttatttt tcagctcaca ctcattaaca tccctacaaa cgtatttata
    36961 ggctatatta ttcttgtcct ttttggattt gggcctctgt tctatttctt tgctgcctat
    37021 gctattgtat ttaatgtcgc agtaacaggg cactatatgg cacagcttca atatgaagta
    37081 ttgggatacg ataaaaacaa aatggacttt taaaaaatca tttaaccatt tcacgtagct
    37141 tttttaactg gtcacggatt tgtgccgcac gctcaaagtc aagaattttg gcagctttgt
    37201 gcatgtcctt ttcaagttta tcaataagtt ttggtaactc tttctcgctt attcccaaat
    37261 ccaccatttc ttctgccaca atatcttcaa ggtttctgta gcttgcaacc agcgataaca
    37321 aattgttttc tatgggcttt ttgattgtat gcggaataat cccgtatttt ttattgtaat
    37381 taagctgaat ttctcttctt cggttagtct catctattgc ctttttcatt gaatcggtta
    37441 ttttgtcggc atacattata acagcaccgc aagaatttct tgcagccctg ccgattgtct
    37501 ggataagact tgtttgggag cgtaaaaatc cttccttgtc cgcatccatt atcgcaacaa
    37561 gcgaaacctc aggaatatca agcccttctc ttaataaatt caccccgata agaacatcaa
    37621 actctccaag acgcaaatcc cttaaaattt ccacccgctc cagtgattga atttcgctgt
    37681 gcaaatacct gactctaacc ccctgttgaa gaaaataatc gcacaagtct tctgccattt
    37741 tctttgtaag cgttgtaatc aaaacccttt catttttttg agcccttaaa tcaatttctt
    37801 ttattaaatc gtcaatctga gtttcaatag gtcgaaccga aattttagga tctacaagcc
    37861 ctgtcggacg aataatttgt tccacaagct gcgctgaagt ctgtttttca aactcagcag
    37921 gggtagcaga aacataaatt ttttgatgaa ctttgtcaaa aaattcattt tccttcaaag
    37981 gtctgttgtc ctttgcacaa ggcagtctga agccgtattc tacaagggtt tctttccttg
    38041 ccatgtcccc tctgtacatt cctttaagct gaggaagcgt tacatgcgat tcatcaatca
    38101 cggtcagaaa atccccacgg aaataatcca aaagcgttga aggcgcagac cctacgggtc
    38161 tgcgctctat tattctcgaa taattttcaa ttcccgaaca gtagcccatt tcctttatca
    38221 tttcgatgtc gtaatttact ctttgctcga gcctttgggc ttcaataagt ttattttgct
    38281 cgtttagcgt agctaaacga gccttcaatt cttctttaat aagccctatg gtctcatcaa
    38341 cgttttcatc gtaggaaaga tagtgaacgg ctgggtatat aacggttttt tgcggagttt
    38401 caagtatttc tcctgtgaca gaatcaattt ttactatttt ttcgatttca tctccaaaaa
    38461 aataaattct tgtgacaatt ttttcataag cgggcatgat ttcaactata tcaccacgag
    38521 ccctgaaagt agagcgttcc aaaaccaaat catttcttat atatcggact tctacaaggt
    38581 ggcgcagaaa atcttgtctg gaaatttctt ttccgacaga aagttcaatt gagcctttaa
    38641 agtaattttc aggtaacccc aaaccataaa tacagctcac agaagccacg attataacgt
    38701 catttctttc atacaaactt cttgtagtat tgtggcgaag gcggtctatt tcttcgttta
    38761 tgcttgaaga tttttctata taggtgtctg tgcgcgggat ataagcttct ggctgataat
    38821 aatcgtaata gcttatgaaa tattcaaccg cattgtcggg gaaaagttcc ttaaattcgt
    38881 tataaagctg tgctgcaaga gttttgttat gagcaattac aagagtgggt ttttgaacct
    38941 gctcaataac atttgcaatg gtgaaagttt tacccgaacc tgtaatccca agcaaagttt
    39001 gcgaatcatc accgcaatta agccctttgg tcagcttttc gatagcctgc ggctgatcac
    39061 ctgaaggctt gtattttgaa tggattttaa acttattttc cataagaata ttttaacaga
    39121 ctatcgataa ttttggaatt gcagaggata atcgtatttc tcttcgtttt ctctcaatat
    39181 ttttataacc gcctgcaggt cgtctttgtc tttgccagaa actcttacct gatcgccctg
    39241 aatagatgcc tgaactttta tttttgaatc cttgatgtca gaaacaattt ttttggcaag
    39301 ttcttgggtt aaaccttttt taagtttata aacctgtctt acatttccgc ccaaagcgtt
    39361 ttccatcggc tgagggtcaa gaatttttat actcaaattc cttttaacta actttgactg
    39421 caaaatgtcg agaatgtttc ttaatttcat atcatcacaa gttgttattg taattgcttt
    39481 gtccgcttca agctcaactt ttgaattgga attttttaaa tcaaaccgcg atataatttc
    39541 tctttcaacc tgatcaacgg cattaaccaa ttcttgtctg tcaaattccg agaccacatc
    39601 aaaactgcat tcttttgcca tacaaccctc ctttttttcg attgtatcac aaaaacttaa
    39661 gggcggaaat ttttttaaaa aaatttccgc cctttgtttg gtttatatta ggatttacta
    39721 aggtaaaatg atttcccctt ttggtttttg aacatatgca ctttcgatga taggctctgt
    39781 aggcttatga atataagcac tttcaattaa aggttcttta gggatattca ccgcataagg
    39841 tgttgtaata accggctctt ttggaacatt aaccgcataa ggggtttcaa ttattctttt
    39901 tgttgctctg acactatttg agccttcacc gatgaaaacg gtttctgatg cttcttttct
    39961 aagcttattt tgttctgccg tatattccca ataatgattt gtttttactt tgcttggact
    40021 tttcggtctg ttataaaaag ttgcaacatc gtcagaagta ttttttataa cttctatagc
    40081 cgattttgaa gcctcttctg caacatttgc agcttcttga ataccttttt taattgtttg
    40141 aggagttttt attttgtatt ttcctcttgc caaagtgtat tttgattttt tagcaactgt
    40201 atcagcaact tgttccaccg cttctgtagc aggttttgca acatcatcta ctacatttgc
    40261 aactgcttct gttgcaggtt ttatagcttc ttgagccgct tttgcagctt cttttactgc
    40321 ctttgtgccc tttccttttc ttaaaagtgc caaagagcct attaacaaag ctatagtacc
    40381 tattatggtt ccggccttaa gtgcagtatc atccttttct ctgtgaacaa aagcatcaag
    40441 aggtgccggt cttccaagat aattcgtgtt gagcccgccg tttacgccat atcccggtac
    40501 tgtcatcccc ggcattgtca ttgtattcat caaataagga ttaaatcccg ttgttcccgg
    40561 attaattccg taagtattac cgtactcgtc aggcaaataa ttcataacct taacctccaa
    40621 tataaaacca aaccttatct ataaaaaaac aatcaaagcc tgattattgc atgtgcttta
    40681 tgagttttaa gatttttaaa atgcaaaaag tattttaaat caagtgttta caggattgta
    40741 acaaaatatt acgcaagaga atttagtaca aaatttattt atcacttgac caaaaatttg
    40801 cttaattttt gtgtttttga tagactgaac atggaaaatt agtttagaca agatgaggat
    40861 ttttttatga gcttgatgaa aggtaaaaaa ggggtaatat tcggagtttc caaccaaagg
    40921 ggaatagcct atgccatagc agaacaatta cacaatgcag gggctgaaat tgccttcacc
    40981 tatgccggtg aaataatgga aagcagagtc agacctcttg cagaaggcat gaatgccaaa
    41041 ataattatgg aatgtgatgt cacaaaagaa gaacatgtta aaaaagtttt tgaacaatgc
    41101 gaaaaagttt acggaaaaat agattttgta gtgcatgctg ttgcctttgc aaacaaagaa
    41161 gatttaatgg gcaagtttat cgatacaaca aaagaaggct gggatttagc acttggagta
    41221 agtgcctact cgcttatttc aatagcaaga aacgctcaac cgattatgaa tgaaggcggt
    41281 tcaatatttg ccctgaccta tcttggttct gattatgttg ttgcaaacta caacgtaatg
    41341 ggtgtggcaa aagcggcact tgaatccagc atgagatatt tggcagaaga tttaggaaaa
    41401 tacggtataa gggttaacgc aatatcagca ggtgccgtta aaactcttgc ggcaaaagga
    41461 attgcaggat ttgataaaat gctaaaagca gcagttgtta aagcaccgct cagaagaaac
    41521 gtaagccttg aagatgtagg taaagcaggg ctatatttag catctgacct ttcttcaggc
    41581 actacaggac aaattttgtt cgtagattgc ggatgtcact gtgtttatgc ttcaggagaa
    41641 gaaatggaac ttatttccaa ctgtattcag gcacaatcat agtattataa aataacaaaa
    41701 gggcggattt tttcaaagaa aaaatccgcc cttttgtatc atttaaaatc atcgggcttt
    41761 ttatctttca gccacttttt catgacgtgg tgttcaaagc ttagcgcaaa attataaagc
    41821 gcattggtaa gcttttgtcc gctgggtgac tttgcaacaa gcaaaaaatc gccggcttta
    41881 ttttttgcaa gagaaatttc tgcatgaatt attttttcaa cgtttgtttt gggtttgcgc
    41941 tcaagaattt ctttgagcca aaatcctaaa agctttgtgc aagattgttt ttcaataatt
    42001 tcatcttctc tttcttgcag atatttggca aattcggata aaatcataca caaactccgc
    42061 tttgggcaaa gggcgtagag gctgaaaggc aaacaatatt ttctattccc tgatttttaa
    42121 gttctaatat tatattttca aaagtcgcgc ctgttgagca aatatcatcc ataagcaaaa
    42181 tgtcgccttt caaaagctta tttttgttga ctttaaaggc tcctgaaaga ttttgcatcc
    42241 tttggtcttt ttttaaatta tactgaggct ttgtgtcttt aattctttgt acaagatcca
    42301 aattaacctc gtaaccagtc aattttgaaa attcctgtgc gacaagcacc atgtgattgt
    42361 attttctttg tttttccctt gctttgaaca taggaacggg aactatttga taacatttat
    42421 ccaaatcacc cgtttttagc caaaaagtgt gcataaattt tgcctgatag taggctaaat
    42481 ccctttgttt atggtattta attcctctta taagtttttg caattcgcct tcataaacac
    42541 ccgcacagaa cacttttaca ccgcaaattt ctctttgctc gaattcgggc agatgctcaa
    42601 ggttagaaaa gcactttgaa cacattttta cgctctcttg tgaagaagaa caaaaataac
    42661 atttcttttt ataaatccaa tccagacagt atgaaataaa ttttttcata gaaaaattat
    42721 acaatagata tttacatgtt aaaatatagt ttgaggagag aaaatgtcaa ttataataat
    42781 gcttcttctt ttaagtgtat taattctggt tcacgaagcc ggacacttta ttgccgcgcg
    42841 ttattttaaa atcaaagttg acaaattcgg attcgggctt ccgataggac cgactcttta
    42901 tgaaacaaaa tggggcgata caaaaattct tattcacgca tttttgttag gcgggtatgt
    42961 cgcattccca gatgacgaaa aagattgtga gctttcaaaa gattctcccg aaaggtttat
    43021 taataaaccg gtttaccagc gtgcgattgt agtttcagcc ggtgtttttg caaatatctt
    43081 atgtgctttt ttctttgtat ttttaacagc cgccctttgg ggaaatcttc cttctggcaa
    43141 gtacgaaata tatgtaaacg acatcgttgc gcccaaacaa gaaagcattt ggcagtcagg
    43201 attacaaaaa aaagacagaa ttattgaaat taacggaaca aaagtagaga acacatacgc
    43261 gcttttagat tttataaaaa tgagcaaatc ctatgacgga aaaattgatg caaatacaaa
    43321 aacggacaat tatttgcggc taaaagccct aaattctgtt tttacacaag atgaaatcat
    43381 acccgaaggt attgcggtca aattacctga aaaaactttt gaagcaccgg taaaaataga
    43441 acaaaaagtc ttaaaaggaa tgaaaaaaca cgttgataaa cagatttctt taaattccaa
    43501 acagacaaaa ttaagagata agctcatcgg acaaacagct tacatttcag acggaacttg
    43561 cactttagat gatttggctt atgcgatttc cgataattca agacctttgg atataaaagt
    43621 tttaagaaac ggaaaaacaa ttaatctcaa aactgtttat gcaaataaaa aagggctttt
    43681 aggaataaaa ttggaagcaa aagaggtttt aatcccgaca aagacccctc aacaagtaat
    43741 tacaaaaggg acaaaatacc tttgggacaa cacttatatg cttttatacg ggctttacca
    43801 gatcttcagc ggtcaaatcc cgcttaaaga cctgcacgga attgtcgcaa taacaaaagt
    43861 gggcggagat ataattacaa acagcggaat tttcttcgga ttgcttttaa tcgcaataat
    43921 ctcaatggat ttggcaataa taaacttcct tccgattccc gcactcgacg gcggacacat
    43981 aatgtttttg attatagaaa agcttcgcgg aaaacccctt gacgaaaaag ttatcgacaa
    44041 aataggctcc gtcggctttg cttttctgat aacattaatg gtttttgtaa tctttaacga
    44101 cattgcaggc ttaattacaa aaaaattcta attttataac gaatttgaaa aatacagcaa
    44161 atatatggtc taaatcccgc taaattgtat tatctttgta tgataaagtg agggctatta
    44221 tatggttgat aaaattacaa ttgaactgga tgaacaattt cttaaaaagt ttttcgggct
    44281 tacggaaaaa tttatccccg aagatcagga aataactctt ttgatgctaa gaaagcaact
    44341 cgaagaaatt gaagaagcaa tatttaatgc aaaaatgacc tcttctgctg ctgcggctgc
    44401 gcctgcatat caaaaaccat caggtccgca agctccgaca aatgtcgatg cctttggcga
    44461 agaaatacat aacggagatg agttcaatgt acctaaaaaa agtattttga taattgatga
    44521 tcttggggtt ataacttacc aactcagtgt tttatttagg aatatgggtt attacgttgt
    44581 tgactcaaaa gaaatttatg atgccataga aaaattcaaa aaaaataatt tcgacttggt
    44641 tattatggat ttatttatac cgaccgaaag agaaggtttt atcttacttg aagaactgaa
    44701 aaagacttct ctaagcaaac aaaaacaagc aagaataggt attatgtcgg cctctgccaa
    44761 aaaagaacac aaacaaatct gcaagatgaa aggtgccgaa ttctacgtcg aaaaagtaga
    44821 tgactggcaa aaagaactta ttaatgtaat tcaaaattta agttagcctt ttaatgcgtc
    44881 tgctcctgat acgacttcca aaagttcctg tgtaattgca gcctgacgtg ctttgttgta
    44941 gtcgattgta agtgttcgta tcatctcttc ggcgttattt gaagcggcag acattgctgt
    45001 catccttgaa gccaattcgc ttgcctgcgc ttccaaaagc gattgaaata tagtatttgt
    45061 aatatacatc ggaacaactt tttgtaagat actgtgttca ttaggttcaa aaatcataac
    45121 aggatcaatt tttaccgccg attctgtttt taaatcttct tcagcattta taggcaataa
    45181 attccatttt tctgccaaat aagacatcat atttttgaat tttgtcgtaa aaatttctat
    45241 agtgtcaatt tctccggaaa cgtaagcctg agaaaggtct tcagcaacat attgagcacc
    45301 tgcagcagaa atttcgcctg ccacttttat ataagttttt acgatttcaa atccgtaatc
    45361 ttcggctttt ctttttaaag ctgcatttcc tttttgtccg ataataaaga gtttacagtt
    45421 aattccttgc tgtttatatt cttcaattgt tttaatagta tttcttacca cgtttgcatt
    45481 ataagcacct gcaagtccct tgtttgaagt tataaccaaa agccctgctg ttttaacttc
    45541 gtgctttttg agcaattcag ggtaattatc tattgccagt ttaattttta aaccttcgga
    45601 agaataatca ccgactgccg acaaaagctt tttgaaggca attatcagct cctcgctgaa
    45661 aggtcttgaa gcttttaccg cattttccgc ttttttaacg tttgcggcag caaccatttt
    45721 catcgcacgg gtaattttct tcgtgttctg aacgcttagt attctgcttt ttatgtcctt
    45781 aagatttgcc attattatcc tttttgagtg aataagtgta ttttttgtta ttatattttt
    45841 ttgcgaagca aataattgaa cctattcacc tatttactta ttcacttatt tacttctata
    45901 tttttatcct ttaaatttca atatagcatt tttcagagct tctttgtctt catcagaaag
    45961 tgcctcgccc gcgttaagcc tttggcttaa ttcaggcatg ttggcatcga aatattcaaa
    46021 ccagcctttt ttgaaagtcg gaatttctct gttttcaact tcatccaata agccttcgtt
    46081 tgccgcaaaa agaacagtta cctgttgggc aacgcttagc ggctgatatt gaggctgttt
    46141 aaggacttct accagttttt gacctcttaa aagctgttgt tttgttgctt tgtcaaggtc
    46201 agaggcaaac tgtgcaaaag cttccagttc tctaaactgc gcgaggtcaa gccttagctt
    46261 acctgcaacc tgtttaatac cttttgtctg agcagctccg cccacccttg aaaccgaaat
    46321 ccccgcgtta atcgcaggtc ttatgccggc gttaaacaaa cccgattcca aaaatatttg
    46381 tccgtctgtt attgaaataa cgtttgtcgg aatataagct gaaacatcgc ccgcctgcgt
    46441 ttcgataata ggaagggcgg tgatacttcc accaccaagt gcgtcagaga gcttgaccgc
    46501 tctttccaaa agtcttgagt gcagataaaa aacatcgccc ggataggctt cacggcccgg
    46561 cggtcttctc aaaagcaaac tcatcgcacg gtatgcctga gcgtgttttg tcaaatcgtc
    46621 ataaattatt aaaacatgtt ttccctgttc caaaaattct tctgccatag cgcaacccgc
    46681 aaaaggcgca atatattgaa gcggcgcaga ttcattcgcg cttgcggcaa cgataattgt
    46741 gtattccata gctccgtaag attccaaagt tttggcaagc tgggcaacgg tagaagcttt
    46801 ctgaccgatt gcaacgtaaa tacaaattac atcctgacct ttttggttaa ttatggtatc
    46861 aattgcaata gcggtttttc ctgtctgtct gtcaccgatg attaactccc tttgacctct
    46921 gcctattgga gtaagtgcat caatcgaagt taaaccggtc tgaagcggct gatgaacgga
    46981 ttttctttta acaataccag gtgcaacttt ttctacagga agggttttat cggcttttat
    47041 ttcgcctttt ccgtcaatgg gctgacctgt gggatcgact attcttccga taagcccgtc
    47101 acctaccgga acagaagtaa ttcttccggt tgtttttacc agcatacctt ctttaatatg
    47161 ctgatattcg cccaaaatta caaccccgac gttgtcttct tcaaggttaa gggttattcc
    47221 caaagtacct ttgccgtctt gaaattctat aagctcgttt gccataacgt ttctgagccc
    47281 gtaaagcctt gcgatgccgt cgccgacttc aagcacggaa cctacatttg aaatttcggt
    47341 ttgagattcg taattttcaa tcttgctttt gattattgaa ctgatttcgt ccggtttaat
    47401 tactgccatg tctttttcct cattatcttt attttgttat acttttgctt aaatcttcca
    47461 atttgtgctt caaacttgtg tcaattacgt tgtcgcccat ttgaattaca agtcccgcaa
    47521 tgatttcagg gcttatttcc caatcgaaag tcactttttt acttaattta ttttccagtt
    47581 tttcttttaa ccgttttttt gcatcctcgc ctaaagcaac ggcggaggtc actttaatcc
    47641 ggcttacgtt gttgatttta tcaagaattt tgtcgtaagc ttccgaaacc gcttcaaact
    47701 ggcaaaatct gtctttatct ataagaattt ttatgaaatt caacactaaa gtatcaattt
    47761 catctgtaaa aactcttgct gcaacttctt ttttatcttc aatagaaatc agaggatttg
    47821 ttaaaaattc ctgcaggtcg aaagatgttt tgagagtttg caaaacggtt tttatatcag
    47881 ccgaaacttt ttcataagta agttttccgt ctttgaccaa ttcgaccaaa gcttcggcgt
    47941 atctttgtgc tattagaatg ttttttgtct gattatttgc caaaatttaa cctgtccaat
    48001 tcttctatac tttcgtttat gtatttttcg tgcaattcag ggttgctcac aagcgaattt
    48061 gtaatatggc tttttgccgc ctcgatagaa gcctttgatg ccttttgagt aagttttgaa
    48121 ataatcattt tttcttcggc atcaataatt ttttttgcat ttgtcccgat attttcaacc
    48181 tgttttttag cgtcatctaa aatcttttct ccaataacat gcgcacttgc ttttgcctct
    48241 tttatcatct cctcaagctc ttgaggaaga ttttcgacta acttttcaac ttgtttcaaa
    48301 tttgcatgtg cttcttcctt gctttttttt gccgcttcga tagcttcgac tatttttgtg
    48361 tgaagcgatt cgatagcttt ttttatgtcg atttttttga aaatccaaat aaaaattgca
    48421 gcaaaaaaga taaaattgaa ggtgtttgat tcaactataa tatgccatat ttgctgtatt
    48481 tgttgattaa tcatctttta tcccaatatt tttgtagaaa ttgtctgcgc aagttttttt
    48541 acttcatctt ccaaaacttt ttgtgcctca gcttttgact tttcaagctc ttcttttgcc
    48601 cctgaaactt cttctgacgc tcttttttgg gcgattccgg ttaagtccgc tttttgcctt
    48661 tttgcgcttt ctgaggtatc aaaaattatt tttttggctc ttgttttgga ttcttcgact
    48721 ttgttttctt tgtcggcaat taatgcttcg gatttttgct ttgccaaatt tgcctgatga
    48781 tactgctcat caagaaattc ctgcctttgg ctgactattt tgctcaacgg tttgtagaaa
    48841 atcccgttca taatcagcac aaatacaata aaacttattg ccgaaactat aaatgttgcg
    48901 ttaaattcca tttttgtcct ttttagttga aaagtcgaac agtcgataag ttgaaaagtt
    48961 tatgagagta taagattatg agtttataag ttttcttctc actcctcaca tctcacctct
    49021 cactacttac cccatcatgc ctgacagttt aagcgcgata acaaaagcgt aaagtgcgca
    49081 agcttctgat aatgccgcac ccaacaagaa aaatcctact gcttttcctg ctatctctgg
    49141 ttgtcttgca actgattcca aaaatccttt tgtagcaacc ccgataccga gccctgcacc
    49201 cactgcaccg aatccgattg cgatacctgc acctaactgt gccatttccg ataatgtttt
    49261 aatttcttct gctgccataa tttttctcct tttttattct aatttctatt tcctattttt
    49321 agtgatgttc gggttcttca accgccattg caatgtaggc gatagtaagc atcataaata
    49381 ccattgcctg aatacatgct accagcactt caaaaaacat tataggaagc ggcaggaagt
    49441 atgcgcacat tcccaaaagt gccgaaacaa gtatttcgcc tgccaaaatg ttggcaaaaa
    49501 gccgcagtgc aagcgttagc ggtcttgtaa acatatcaag aacttccaca agagcaagaa
    49561 ttatcgatat aaaatcaaac ccgtgcaaaa agtatttcgg gccttttttc tttactccgg
    49621 taaaaacata ataaaccaaa acaatgattg ccatcgccgc tgtcatgtta atatcgtttg
    49681 tcggggatgc aagctctcct tgcttaagct gaataagcct taagggaagc tgccccatta
    49741 aatttgccgt tatgataaat aaaaacaaag aggcaacaag aggaatgtgt tttctgcctt
    49801 cttttcctat catagaatcg gtcaagtccc agaaaaatcc cattaaactc tctgaaaatg
    49861 cctgaatttt tgaaggaata agagttaatt tgcgggtggc aatcagcgca aaaataataa
    49921 gaaacgccat tgcagtccac atagtcacca atgtgtccat attgaaactg taattaccaa
    49981 cctgcgctat ccagtgccct gcttcgctca tctacttccc tcgctatggt tattgtagca
    50041 tttaattttg ttttggcaaa ttatttcgat atataaatat gaaacaggtt caggcgcctt
    50101 ttcttttccc gcctctttct tatgatataa tcaatcaaat gaagttaatc ttttttgaaa
    50161 aaatagattc taccaacact tatgccaaat taaacattga cgatttggaa gataaaagcg
    50221 ttgtttacac gaattttcaa accagaggca ggggaagatt tgacagaagc tgggtaaatc
    50281 tgggctctga aaatatctac atgacaatta ttcttaagcc ctccgaaaaa tttgaacaaa
    50341 actattcgaa tttaacccaa tatttatccc tttgtctttg caaacagctg aaagaaccgg
    50401 gattaacccc ccaaataaaa tggccaaatg atgttttggt aaacgggaaa aaaatagcag
    50461 gaattctggc tgaaactgtc atcaaaggag gcaaccttaa gggaatagca cttggaatag
    50521 gggttaattt aaatgctgat ttacaagatg ttttaaatat tgaccgccca gccacaagtc
    50581 tgaatcttga attgggcaaa aatattgata aacccaaatt tatgaaaaaa ttactggaag
    50641 aattttttga aagctatgac gattttttaa agcatgggtt taaatttata aaagaagatt
    50701 acgaaaaaag ggcatgcttt ttaggacaaa accttaaagt agtaattttt gacataataa
    50761 aagaaggcta ttgcaaaggt cttgatgact ttgggaattt aattctgaca gacaaaaacg
    50821 ggcatgaaca aagtattaat atgggcgaaa taattgaata aaaaagggcg cgaaagttaa
    50881 aaactttcgc gccctttttt ataatattct aaacccctac aggatttttt gttttattaa
    50941 tacattcaac aaccgtattt acgaccgttt tttgctcatc aagcgtcaat tcagggaaca
    51001 tcggcaaaga aataacgact ttggtatcgg cttctgttgc aggaagcgag ccttctttaa
    51061 cacccaaata ctcatgaact ttttgcaggt gcaaaggtac ggggtaataa agcatagcac
    51121 ctattccagc ctcttgaagc attttgtgaa cttcatcgcg gttaggaatt tttatcgtgt
    51181 actggtgata aacgcagtaa gtgtcatcca actcttttgg tgtttgaacg tcagcgcagt
    51241 ttgcaaaaag ttcattatat ctgtaagcat tttggcgtct ttttttattc cattcgtcaa
    51301 tatgtttgaa ttttactctc aaaattgccg cctgaacctc atcaagtctg ctgtttacgc
    51361 cgatttcgtc atggtagtat ctgacagctc cgccatggtt tcttaagcca ataactctgt
    51421 cttttatact ttgagaattt gtggtcaaca gtccgccgtc acccatagct cccaaatttt
    51481 ttgtggggaa gaaactgaaa catcccacat cgccaaatga acccaccatt ttgcctttgt
    51541 attttgctcc tattgcctga cagcaatctt caattacata aagattgtgt ttttttgcaa
    51601 tatccatgat tacatccata ttgcaaggct gaccgtaaag gtgaacggga attatcgcct
    51661 ttgttttagg tgtaatggca ttttctattt tctcaacatc aatgttaaaa gtgtcagggt
    51721 caatatctac aaaaaccggt ttggcaccca caattccgat tgcctctgtc gttgcaacaa
    51781 aagtaaaagc tacagaaata acctcgtcgc ctgctcctat gtcaagtgct ctaagtgcaa
    51841 gatgaagcgc atctgtgccc gaattaagcg caatggcatg ctttactccg atatattcag
    51901 aaagctcttt ttcaagttct ttattgtgat ttcccaaaat ataagaacct gagcgcaaaa
    51961 ccgctacaac ttctttttca atctcttttc caatcttgtc atactgtctt tttaaatcca
    52021 aaataggtat cataactatc tcctttattt caatttttcc gcatcctcta ttttaagttt
    52081 atcacacccc cgccaagcct ttatattaac ttaatatgaa cctaaaaagc tgaagaataa
    52141 accccctctt gataatctct ttgttacaat ttcttaacaa aagttttttt ctgaggcaat
    52201 aacttttaaa aaaaaaactt tatataagag taagggaaaa ggaattataa ctttagggtt
    52261 aaatttcaaa taatatgttc ataacaatga actattaaaa caaagatttc attttatact
    52321 ctctctctta atatcctcgt gtttatttaa tgtttgctcc aaaagggcaa actttttttt
    52381 gcaagcgagc agaggcacga acgaagtgag agtcgaacaa caataatcgg aaaccacgct
    52441 ttccgatttt gttgtgagcg ttgccgttta atgcgagcgc aggtgctacg cacgtagcca
    52501 ctctgtatag attgatgaaa attccctcaa cccatgtctt ttggctttta gaaaatgcgc
    52561 cagtgtgtat tttgtttatt gcgagcgcag aaagacgttt ttaaaggtta tgctgccaaa
    52621 gcagcaaaaa aacatgcttt aataaatcat gttaaaataa tatctatgac taaattaaga
    52681 atttttctcg ggtatttcat tttaattata atctcgctga gcatgctttt gccgtttttt
    52741 gcgatgctga gtctttcatt catgccggga agcgaaattt tttcctatcc gccaaaattg
    52801 ttttcaaaaa atatcacggt gcaaaattac tttagcgttt ttacatcaat ccccgttaca
    52861 agatattttt taaacagcct ttttgttgca acaataacca caataggaca agtttttttc
    52921 gcagcacttg cgggatatgc ttttgcaagg cttaaattta aaggtaaaga tattttattt
    52981 tttattttct taataaccat gatgattccg cctcaggtga atataatccc gcttttcttt
    53041 ttaatgcgcg aacttcatct ggtggacact tatcaagcac ttattatacc tggctttttc
    53101 ggcgggtttg gagtttttat gctcaggcag tattttttga atttgcccaa agaactggaa
    53161 gaatcttcca aaatcgacgg ctgcaatatt tttcaaatgt tttttaaaat cgcgcttccg
    53221 cttgcgctgc ctgctgttgc aacccttgcg atatttactt ttgtatcaag ctggaacagt
    53281 tttatgtggc ctttgattat aacaagctct gattcaatga gaactttacc tgtgggtttg
    53341 gcggctttta aaggaagttt cagagaagtt accatgtggg gggatttgtt ggcatgtgcg
    53401 gttatttgca ctttgcctgt tgtcggaatt tttcttctgg gtaaaaaata tttcataaac
    53461 gatcttttgc aaggcggggt taaagagtaa aaagtaaata gtaaatggag agcgggcaaa
    53521 aacctttccg cccattcacc gaatataacc cacctgacca gtttaaatat attcttaaat
    53581 ctattatcat cctaggtagt cttttgttca aagttcagga ttaaaaaatg tcagaaacaa
    53641 attttattta caccaaccaa aacggtgtga gtgaggcttt atacaataat tttttgtttg
    53701 tagaaaatac agcaagactt aaaagcatta aaaattttga aaaaacaaaa ctttgcgtaa
    53761 taaaaacgga ttttggcaaa aatgatttaa aaacgataga aaaaatcaaa tcggaaaacc
    53821 aagacgttga attttggatt tgttccaaca attcttcaag aaaaaacatt ttagccgcaa
    53881 acaaaatagg aataaatacc gttcttgcat gcccttttga tatttcaatg attgaagaat
    53941 accttaacag ccaacaattt ccttacttaa ataacctttc cgaaaacaat tacagcggac
    54001 tggaagggct taaggtgatg atagttgatg ataatgtaat gaacgttgaa cttttagaag
    54061 aggttctatc agagcttaat ttaaggatat caagtttcac aaagccggct caagcaatgg
    54121 aatttgtatt aagggaaaaa ttcgatttat ttttgcttga tattatgatg cccgaaattt
    54181 cgggatttga tttgattaaa atcattaaaa aaaccccgct taaccaaaat gccccgatta
    54241 tttttatcag cgcactttct gacgcccaaa acaagataaa aagttataat ttggggtctt
    54301 atgcttatat cgaaaagcct tttgacataa acatagttaa atcacaaatt tataacattt
    54361 taaaaaatca taaaattcaa gaaattctat cttccgacaa agaaactttt cttgccaccg
    54421 tagctcatga cctaaaaaca cctataaagg ctgaaattaa tgcgctaaat atgctcttag
    54481 acgacaagtt tgataaaatg gaagttgata aacaggaaat tcttgaagat attttaagtt
    54541 caacacgctt catgcaagat ttggtggaaa atctgctttg caaaaacaag attgaacaag
    54601 aaaaaatcaa cctgtgcaaa gatttttact gcgttaaaga acttgtttta cactgtattg
    54661 aattgacaaa atatatttta ttgcctaaaa aacaaaaaat taattttaaa tgcaaaacag
    54721 aagaaacact gctatttttt gattttttag aaatgaaaag ggctcttcac aaccttattg
    54781 caaatgccag cgaatactca agcgaaaaag acgaaatttt agtagaaatt tttaaagaag
    54841 ggaacaattt gggtctttca gtacaggatt ttggcagggg cataaaactt gaacatcaaa
    54901 aagatgtttt ttcacagtac atgtcgcttg ctaaacagca aaaaacagta ggttcaggct
    54961 tggggctcta cattacaaaa agcattgtag aagcccatca aggggaaatt ttgctcgaca
    55021 gcaaagtcgg cgaaggtaca aaaattacaa ttatgcttcc gatttatact aaagtttaaa
    55081 aaatattatt atttcagata ctggtataaa tctgaataaa tgttagaata ctggtggtta
    55141 atatttccac agataaggaa aagtatgaaa aaacaattgc cgtatgaaaa aaattcaaac
    55201 tgtattttta aactgaattc tgacaatatt tcctcgaatt tggtttacaa acttaaaaaa
    55261 acattcaaaa aactccctaa aaataaatcc gttgcaatta atttacaaaa cgtagaatat
    55321 gtttgctcgg aatttttaga atttttaaaa gaattttcaa aaaaaagaga aatttcacta
    55381 tttaatattc aagctgaaat ttcggctctt ttttacctga caaaatatga caaattcgcg
    55441 cacatttacc ttaacgatat agacttttac gaacaaaaaa gaatgctttt gaacagaaac
    55501 ttttcgattt taaataactt taagcaataa ttaattacaa tttgttttta taaatatata
    55561 gacttcaagg ggttgttatg ttcgattttg tacaaaagtt tatatattgg gttttgtcgt
    55621 tgcaggaaaa acggttaaaa cacaaattag gcggcagttt gaaacgttct ttttcaaact
    55681 ctacttcaaa aattgttttt tcaaaatcag acagtcttaa acttaattct atgactgaaa
    55741 agaacaaaga aagacttgaa aaagaagtaa aaataatatt gaaacattgc gagaataatc
    55801 ccgataaagt ttttgagttt attgagaaaa aaggtacaaa agtccacaaa attccttttg
    55861 ctcacaaaat tttaaaatta ataggccttg aagaagggct tgttgcaaag cttaacggaa
    55921 taaaagcttt gtatttaacc tctgtgcttg ctgttttagg caaagaaaat aaaatctcat
    55981 taactacaaa accaatgttt gtcatgaggg atttgccgct aaattcatat tacacaattc
    56041 agcaatttca caaatggtat gcaacaaagc ttgatatgcc tggatttgat gcaaaatctc
    56101 aggaacattt ccaaaaatat ctggacaata tcaatgatga aagtataaaa agtttgagca
    56161 tggaagaaat tatcggactt aaagaagcta ttgcaagaga cgttgaggcg ataaattttg
    56221 taataagcct tgcgaaatca acagaaggtg caaaaaacgc cctgaataaa atggcaaccg
    56281 aaaaaggtgc atctgtttaa ttaaataaaa ataattacaa aacaatctac tcgtttaaat
    56341 attttgcagc acaatcaaaa ccgtttcctg aaggacattc tatttcggtt ggggccaccc
    56401 aataaccata agggcataat ctgtcgtaaa ttccattacc attacaatca tatattattg
    56461 aaaaaacatc tttacccatg acattaggtg gcttaagccc gttaacatct actaaaaatc
    56521 cggcatttgc tagatcatat gctattgcag ttccatcttt aagaatactt ccgacctttc
    56581 cgtcatcaga aacgtaaaat ccacctgctc cgtcaagtgt tttaacttct tcaacaaagc
    56641 aatttccggc aatagttgaa ttatcacatg attttattac cgacaaatat tgttcaaaag
    56701 ttgattgaaa atcttcattt gttaattcgg aacctagttt tttataaaaa tccggatcct
    56761 cacttaaaac ctgaattgaa acctgattaa gagttgaata tgcttttttc aaagccactc
    56821 tcagctcctt ctcgttagtt ctgttaatga ccatggggat tgtaagagct gcaaccactc
    56881 ctataatagc tagcgttatc aaaacttctg ccaatgtaaa tccttttttt ctcatataaa
    56941 aatcctttct gtaaactata ctccgacgct taaccccgca cataaattta ttgactgtcc
    57001 ggtgattcct cttgcatcgt ttgaaagcaa ataagcaacc aattttgcca cttctttggg
    57061 ctcgacaaat cttctttgag gaatcatatc aagggtttct tcaagcgaaa aatcgctttt
    57121 ttcgattgat ttttcaccca aatcggtctg cacccagccc ggatttattg tatttacggt
    57181 aatgttgtgt tgtgcaagtt caagcgcaag ggctttggtg aatcctataa gccctgattt
    57241 tgaacccgag taaaggctcg cataagcctc tcccataacc ccgcttattg agccgatatt
    57301 tactatccta ccccattttt gaactttcat aaaaggaact gcctttgaac acaaaacaat
    57361 aggagaaagc aaattaactt caaaaatttg tgaaattttc tccctgtcgg cattttctat
    57421 gttcgcatat aaatattccc cggcattgtt taccagaaca tttatttcat ttttttgtat
    57481 gaaattaccg agtttttcaa cctcattagg ttttgacaag tcgcaggcaa taaaatcttt
    57541 taccccgagt tcctgagcaa tttttgttaa attagtttcg ttttttgcac ttaaaaaaag
    57601 attataccct aatgccttta attctaaagc aattgccctt cctatacctc tggatgcacc
    57661 ggttatgaga acatttttat tcatttccac cactttcaaa ataaatcagc aaaacagttt
    57721 ggattatttt tcttacaatc tggcactgaa catcttcagg cacatctttt ataatctcaa
    57781 aagaagtatg caagtctata tttgtatcat accaacgctt gcacttgtat gtataatttt
    57841 ttgaaatatt atttattttt tcatccaaat caaaattgaa atcgtttact aaaatttgca
    57901 aaatatcctt tgcaatatct ccctgacgtt gctcatcaag gttctcaata aactcaaaaa
    57961 catcctttaa atcaggattt ttatcatacc accttacatg tttcataaaa aatttatacc
    58021 atataagtgc ggtttaatca aaatattaaa gtttctaaac ttccaaaaaa agtctttgcc
    58081 ccactatatt gggtctgcca ttataataac cggcaaaata tcccccctga accggcttgt
    58141 tttttgcata tccggcataa ctttttgccg ttgaagaagt tacaaaagga ttagaaaaag
    58201 gatttgaatt tgaaaccgga ctaagtgccg ttgttttttg caaattattt gcagaaaacg
    58261 cccttgacaa taaatttacg atgttggcaa ccataatttt cctttcactt tgggatattt
    58321 caaatattaa tgataattta aaataagtcc atgtatatta taggtttaaa ttaacaaaaa
    58381 ttaaactttt ttcaaaaaaa tcatatatct tatgtactga ttttttatta atttacaaaa
    58441 aagtgcggga tgtcattgac atcccgcact tttttatatt taaatttaac taagctgcga
    58501 ttgaaccttt gatttcactg ataagatatt ttgcaatcca agcaaaatct tcattatcgg
    58561 ctgccgcttt tttaaggtat tcaactgaag catcaccgat tctgataaga gtcatagcca
    58621 caactcctct ttttacgcct ttaaccactt gaagctggct gactaattcc ggaacagctt
    58681 gtgctccgat gtttacaagc gtattttcaa tttgattttt aacattatta tctgcctctt
    58741 ctaactgaga aaggaagtaa tgtatcggat tcatattttc aattttgttg attatagctt
    58801 gcatattgtc tccaaatgtt cttttacttg ttattattat agacgaaaaa gcaacccgtt
    58861 tcggatttcc ttacataatc ttaatatcga aaaaagtgaa gagtgatgag tgagcagtga
    58921 acggaatatt tttgaaatct acaatttcaa aattattccg tgccatcaat tttatttatc
    58981 ctgccccttc acttttcact tctcaccctt cacttttcaa gactacccac ttatcttatg
    59041 ggcatgtagt ctaaaagggg attttttatc aaataaaaac atttaaactt atcttacagt
    59101 gggataaatt cccgtactca agatattggg agtgataatt atgttaatgg aaaattgtca
    59161 agctgactgt agcaggcatg gtcatatgga attaaaaaat gttaacaaag aaattatttc
    59221 atggcttgaa gatgtaattg acgaaaacaa cagtcgagtt gaaaggaaag aatggaaaag
    59281 taaatataac agctacgttg tatatgatta cgaaccgttc tgcgccgaag gctttgaaat
    59341 aaatttgtta ataacatcct acgacagtgc ttatcttaat tttatcaaat acctgtatga
    59401 cgaaaaaata aatacaatcc aatatttaaa caattgtata ggcgtttagg taaaaactca
    59461 aaaaggagac caatcggtct cctttttatt ggggtatttg cgaatatttt tttaagaaaa
    59521 agacatcaaa gctttaacag aacgtgctgt ttctctgacc tcgtcatctt cgtgattatt
    59581 ttcagtattt tcaagaacac tgattgcttc ttctttatcc ttcaactgta aaagttcgtt
    59641 aattgtactc attgcaacgc ttgtactcaa atcgttaatt ccttttcctt ttgaagaaat
    59701 tacaactgca agcgaatcct gattgttctt tttaataagt gctttagctg taagtcttct
    59761 tacatcatca ttttcgcaat tagtgccgac tctttccaga acattaaccg attgtacatc
    59821 ttcatgcttt gcaagtgctt ctataatact gtttactctt ttattttcca tatttttccc
    59881 ctcttaaatt gtagccaatt cggctttaag catttcttcc aactccgaaa ccggtcgttt
    59941 tacaagattt gaaaccttat aagtgcctgt aaaagacggc atttttgatg ttattttgtt
    60001 gcttaatttt gccatatcaa tgtgtatttc tgattgttga gcttttgtcc aaaaatcagt
    60061 cgcattttgt ctgattctgt ttccgaaaga cacaactgaa ttcaacctgg tacttattgc
    60121 agagctcagg tttgttattg aacccacaac cgcactggac aacattctgc tcttattttt
    60181 tgctctttct ataagattta cattgttgtt ttttgtttca aatacatccg cggttaaaac
    60241 gtttccctta aagcttacac caaaaggatt ggtgtgattt ttgtccactt ttttaactgt
    60301 agtggttctg aaaaaatttt ttgaaatatt atttatgctc aatttttcca tttcgatacc
    60361 tttcttactt aataactgac tcaacaagaa caaagttatc acaacaaggc acccagtaaa
    60421 tcatcttttc gtaggataag tatataaaaa agctatacta aggttgggtg ctacgcacgt
    60481 agacattctg tgtagaccaa tgaaaatgcc ttcgactcat gttttttgga tttttgcaaa
    60541 gaataacgct gtgaatatac ttttacttct taccgcccac ttcattgaaa attattggta
    60601 atcgtataga tatattcaag aatttgtgag aaatattcta aatcggaatt tccgtttaaa
    60661 atcatatcag cttcgtttct gaagggctta atgtattttt cgcctgcgat tttaacgtat
    60721 tcccagtgtt tttgggcatt acactcgtct tgatttctgc aaacagccct tgacataaat
    60781 cgtctttgtc tttcctgaca atccgcttct atataaattt tgatgtcaaa aatttcttta
    60841 acatccttat acatagttga cattccttcg acaaccacta ttttttgagc ttgtacagga
    60901 attgattttg gcacagaaac acctgtgccg ttaagcaaat actgaggaat ttttatatca
    60961 agaccttttg aaataatttc caaatcttct ttcaataaat caagctgaaa actttccggt
    61021 gcatcgacgt cgtatccgtt atcacgaaga gtatcaaaat ctccccaacg gcttattaaa
    61081 tctgaaatat cgttaaaata attatcggaa cttattattg taacgggaag gttaagtaag
    61141 gctattgtat cttttatttt gttgcatatt gttgacttgc cgcaggctga ttctcccgta
    61201 attccgacca aaatcttttt ttgaggattg tttataagtc tttttgaaaa ccttgaaata
    61261 aaatcgggtc ttacttcaat aaaaataggc gtatggtatt ttttgtcatg ggcaagaatt
    61321 tgcttaaact ttgactgaag ataaaacgcc agcagctccc tgccggtttt ttgattaaag
    61381 tcgggcttta aaattttatc tgaggtatta ttttcttttt caattaatgc tatcatttcc
    61441 cctcatttgt catcctgaaa ttgtttcagg atctctccaa cttacaccgc tgacgagatg
    61501 ctgaaataaa ttcagcatga cgtttaaatg tatatttaag tataataata cttgtaaaaa
    61561 taaaatttgc tataaaatat aacaaatctt aaaaacattt acaatttaaa aaaaattatt
    61621 gtaaaaattt tctaaaaaaa tatcgacgta caagaatttt tttgtacgcc gataaaatta
    61681 ataatatcta aatttttttt aagcttgatt ttctttttct ttaatttcgt cttctttttc
    61741 tttagctgtt tgttcgcctt ctgcaatttt atcagtaggt tgttcgattg actcttttgc
    61801 ttcttctaca gccgcttttg catcttcagc ctctttttct gcgacttcgc caattcctct
    61861 tatttcagcc tttatttctg catttttgtc tttcttgcca ccgaacaaac caaataaagc
    61921 tgctgctgca acaccaactc caataacggc aacggcaaga agagcagggc taaacgctgc
    61981 agcaccgaca actccgacaa cagctccagt tgttgcgtca acaactgctg ttccagcaag
    62041 ctgaacggct attaaagatt ttaccccgac taccgctgct gcaccaattc ctcctactgc
    62101 caatgaaccc aatccgattt ttgccagtaa actttttttc cctgatttac ctttttttac
    62161 tttagcaagt atatctttaa ctgcggaatc tgttgtatca atatcttttg aaactttacc
    62221 aattttatca gcattagtct gaatatcttc tcctaaagaa tctaaagcag atgatttttc
    62281 ttccaattta cttgacgcct ctgtgttgtc tgaattttgt ccggtattat cttctgaatc
    62341 ttctgtaccg gctaaattta aagaatacgc actattttta ccggctccgg ttccatcttc
    62401 tgtcgatatt gatgaaattt catccttaag tgtttgtgct tgctcagctt tagaagaatt
    62461 accagacaca agttgcgaaa tgtaatttgt catttcttga aatgcatcaa cacatttttt
    62521 cataagatcc tttaacccat caaaagaatc ttcttcttcg accatttcag aatcaatgac
    62581 agaatattca gcattatcta tggcttcttc tgcggttttt ccttctgtgt tttcagtaga
    62641 actttcttct gttgtcttta cctcttcttc attttctatt tgattagttt caagagaata
    62701 gttaagtggt tttatattca tcttatacct ccttttttat atttttacga cattttaagc
    62761 aaaaaacttt agttttttgt taagagaagt aaaaccgttt ttaaattgtc ttaaatgtaa
    62821 cagatttgaa aataaattgt tttagtgtat tatgggatta agcaataagg aagatgaggg
    62881 ttttatatgg aaacattggt aaaaaacgag ggcttaatat cgcagattat cggtcctgta
    62941 attgacgtgg aatttcagca gggagaattg cccgaaattt ataatgcttt aaaaattcaa
    63001 aaagacgacg gaagctttgt tgtagcagaa gttcaacaaa tgttgggttc aaataaagta
    63061 agaacagttg caatgtcttc aacagacggc ctcaaaagag gcatgaaagt catcgataca
    63121 aaagattcta ttaaaatccc cgtcggaaac caaattttgg gaagaatcct aaacgttatc
    63181 ggagagcccg ttgatgaaat ggggcctata gaaacaaacg attatctgcc tattcacaga
    63241 gaatccccca agctcgttga ccaaaataca aatatagaaa tacttgaaac aggaattaag
    63301 gcaatagacc ttcttcagcc ttaccaaaaa ggtggaaaaa tagggctttt tggcggtgcg
    63361 ggagtcggaa aaacagtttt gattatggaa ttaatccata atattgcaat ggagcactcg
    63421 ggcgtttcgg tcttcggcgg tgtcggcgaa agaacccgtg aaggaaacga cctttggaac
    63481 gactttaaag aaggcggagt tttggacaaa gttgcactta tttacggaca gatgaacgaa
    63541 ccgcccggag caaggatgag agtagggctc tcggcactta ccgcagcgga atatttcaga
    63601 gacttttcaa aacaggacgt tttgctgttt attgacaaca tcttcagatt tactcaggca
    63661 ggctccgaag tttctgcact tttgggaagg atgccttctg cggtcggtta tcagccgacg
    63721 ctggctactg aaatgggcga attgcaggaa agaattacat caacaaaaga tggttcaatc
    63781 acatcagttc aggcaattta tgttcctgca gacgacctta cagaccccgc tcctgcaaca
    63841 acattttcac acttggatgc ttcaaccgtt ctttcaagac agattgcatc tttggggatt
    63901 tatcctgcga ttgaccctct ggcttcaact tcaagggttt tagaccccaa tatcataggc
    63961 gatgaacact acaacactac aaaacgtgtg cttgaaattc ttcaaaaata cagagaattg
    64021 caggatatta tcgcaattct gggtatggat gagctttccg aagaagacaa agaaacagta
    64081 aacagagcaa gaaaaattca aaggttttta tctcagccgt tttttgtcgc tgaaaaattc
    64141 tcaggatttg aaggcaaata tattaaactc gaagatacaa taaaaggctt taaagaaatc
    64201 atcgaaggaa aacacgacaa tcttcccgaa caagctttct atatggtcgg cacaatcgaa
    64261 gaagccgttg aaaaagccaa aggacttaac taaatatgtc tgaacataaa ttacacctta
    64321 aaattacaac ccacgaaaaa gtggtttttg acgccgatgt ggatgaaata tattcaagaa
    64381 gcatgcaggg cgaattcgga attttacccg atcacattcc ttttatgtgc gctcttgaca
    64441 taggtgtaac aaaaattgtt ataggctctg aagtccaata ttttgccatt atgggcggtg
    64501 tttttcaact taaagacaat caagcactta tcttagctca aaccgcagaa aacggaaaag
    64561 acattgatgc cacaagagcc aaagaagcta aagaacgtgc tcaggcaaga ttaaccgaaa
    64621 gctcggatga tattgacatt aaacgcgcag aaattgctct tgcaagagca atcataagat
    64681 taaaagccgc ttcgggtaat taataaacta tctgtacatg gacggttctt gttcttggac
    64741 ggttgtcaaa atccaccaaa acaatctgct gccatgttcc aagctgcaat gctccgtcaa
    64801 taagcggaac agaagttgaa ttacccacga ttgctgccct gacatgtgca tagccgtttc
    64861 cgtcatgcca tgtcgcatca tgatgatatt ctattcctgt gggtataacc ctttctaacg
    64921 cttcaaataa atcgactaaa acacccggct caaattcaat atttgtaatc gaaaccgtac
    64981 ttcctgccac ataaaccaaa acggatgcat tttgaagcga gtgtctgtaa acactgtttt
    65041 ggacattttt tgtaatgtca attatatcgg tgtgcccctt tgtgtttact acaaattttt
    65101 cattaataac tgtcattttt aaatccgttt tttattacac tttaatttta aaaaagaaac
    65161 aaaataatta caagctatat gataaaatct aaaaaaagcg gcggatttct taaagaaatc
    65221 cgccgcttta acccatatcc cttatccctt aaatctataa ttattctttt gcaaataaat
    65281 tttttatctc aaaaagagaa tcttctatga attccattgt taactgaaga acactttttt
    65341 tctcaataca atcgaaaaga aaatagatag gatcttttga attgttaaat ctcatcctat
    65401 aatcaatttt ttctaaatat acaaaatctt gaacctgctc cgaagacgcc tgttttttac
    65461 gcttgcgaag aggcttgtat tttccgttgc cgttagtatc atttttagca ctgcttgatg
    65521 ccgtaatcat tttttcttct ctctctttat gtcttatata tataaagaat atatattaac
    65581 aaaaattgcc tttttcgatt cgtttttgta aacaaatgtt aaataagtga gggggtaaat
    65641 gtatttagct gaatggctga atgggtgaac ggctgaaagg ttattttagt tgaaaagtcg
    65701 aacagtcgaa tagttgaaaa gttttatatt agtcgggaaa caacaatttg aacttattca
    65761 cttattcact tattcacttt ttcacccatt tacccctaaa cgtacaaatc tcttgcaccg
    65821 ttttggattt tatcgagggt ttctatgact tttgctctga ttttttcatc ttttatgtta
    65881 tttttaatgt gattttctat ggcttcaaaa cctgcttttt tagtttcttc actagcatag
    65941 tcattaagat attcggcaaa agtagccacc gcattaggat cgcaaaatct ttgaattaaa
    66001 ccgacttgcg ccaaatccat aaaatctttt cccacccttc ccaatctgta acagcttgtg
    66061 caaaagctgg gaataaagcc atgttgtgct aactcttcta caatttcatc cagtgagcgg
    66121 gtgtcgccca tggtgaactg ttcggttgaa ttttcatcag agtatccgcc ggggtttgtt
    66181 tttgaacctg cgctgatttg agaaattccc aattcaataa cttctcttct cacttgggca
    66241 gactcccttg tggtcaaaat catgccggtg tagggcacag caagtcttaa tatcgcaact
    66301 attttcttaa actgcttatc gttaattgca tgaggcggat tttttgataa atccgaacct
    66361 gaagcaggct caatccttgg aacagaaagc gtatgaggtc cgactccgta tttatttaac
    66421 aaatattttg agtgttcaat aagagctaaa acttcaaacc tgtaatcaca aagcccgaat
    66481 aatgcaccca taccgaaatc ctgaatcccg ccttctattg ccctgtccat tgtttccaaa
    66541 cgccattgat aatttgcctt aggtcctgaa aggtgcattt ttttgtaaac atcggggtga
    66601 taagtttcct gaaaagactg gaaagtcccg atgccaaaag aagcaagacg cttgaattct
    66661 tctgttttca acggagccat attaacgtta attcttctaa cttcgccgtt cattattttc
    66721 ttttcgtaaa ttttatcaat aatttctttt atataatcaa gattgcactg gtttgtatct
    66781 tcgcctgcca caagcaaaat tcttttgtgc cccatattta ttatctgttc tgcctcatgc
    66841 acggcctcat caaatgtaag ttttcttctt ttaatttctt tgttgtcacg gcggaaaccg
    66901 cagtaaagac aattattaca gcaataattt gaaagataaa gcggtgcaaa aagaactatt
    66961 cttttgccgt aaattccttc tttaactttt tttgccgttc taaaaagttt attcaaaaga
    67021 ttttcgtctt caagattcaa aagaactgct atttcttcgg ttccaagtcc ttttaattcc
    67081 aatgattttg taaggatttc attaaccctt ttttcatccc cttttaattc caaataagaa
    67141 ttaattttag ctacatcaat aatattcata ataaaattct cctctaacac aatagtacaa
    67201 ctttaaaaat aaaatgcacg taaaaaaatc ataaaaattt tttaaaacag ctaaaaaatt
    67261 taaatttcgc aggcttcggc aatggcatta aatattttat cggtaatgac ttcaatataa
    67321 tcaacagcaa ccattccgtt tataataaca tcagaaatct ccattgcagg tctaatgtag
    67381 cgttgcgcgg tatgattcac atcggcaaac atcatatctg cagcttctcc ggtttttcct
    67441 cgtgtagctg cacgattgta ccaacgatct tttattacat caatcggagt aaacacataa
    67501 acccttatgt ccattatctc ccttaagtct tcattcaaaa cataaagccc ttcatttaag
    67561 attactttag cgggcttttt aacttcgccg tcagattttg aaacgcaggt tacaaaatca
    67621 tagttgggcg aaacaacggt attgccctgt tttagttcaa ttaaatgctg tttcatcaaa
    67681 tcaagattaa ctgcttttgg cgtatcaaaa ctaaaacctg ttttaaataa agcttcgtag
    67741 cttcccgcat caataagttc ttttgaagta tcataaaaat aatcatcgca acgaattacg
    67801 gtataaattc cttctttttt ttctttaacg catgctttta cggtgttatc gacaaaagtg
    67861 gttttacccg aagccgattc tcctgcaatt cctataagaa aagtcttttt cttatcatgc
    67921 acgactcttt ttgctatatc aaaaagaaaa ttattagttg tacgcaaaaa caaaggctgt
    67981 ttttcttttc tgtcttcggc aataatttta tttaaggagt caactatatt agccaccaat
    68041 tccatttcat gacggcttga ataaaaatcg taatttgtta aattaaaatt gctttgcaac
    68101 atcctatccg aagtttcttc catacttaca acaacatcat aacttaaact attttatttt
    68161 tccgcttttg gcaacatgga aaaatacaaa aatttacaat tttttactgt taaaaatata
    68221 ctttacttaa aattatgtgt ctattaaaat aaaagaaagc cgtacttaaa aaaggagcga
    68281 gaaaatgaaa aaatcaataa aggatttagg cgacataaaa ggcaaaaggg cactggtgag
    68341 agttgatgtt aacgttcctc tcgacgaaaa caaaaacgtg acagacgaca caagaattca
    68401 ggctattatt ccgacaataa agtttttaca ggaaaaaggc gcaaaaataa ttctcatggc
    68461 gcatttggga agaccaaaag gcgaatggaa tatggaattt acacttgctc ctgttgcaaa
    68521 aaaactttct aaagtcttag gacaagaggt tttattcatt tcaactccga taggtcaagg
    68581 tgccgaaaaa gatttaacag agcttaaaga cggtcaggtt gccttattgg aaaatattcg
    68641 tttttataaa gccgaagaag caaaagacga tgacatgaca tttgccaaag aacttgcaaa
    68701 actaggcgat ttttacgtta atgatgcttt cggtgctgcg cacagaaatc acacttcaac
    68761 atcaaaactt gcaaaagttt taaaaccggc tgtttcagga tttttaatgg aaaaagaaat
    68821 aagatgtctg ggaaatgccc ttaataatcc cgtaaaacct tttaccgcaa tagtaggcgg
    68881 tgctaaagtt tcgaccaaaa tcggggtttt ggaaaactta atcgataaag tggacaacct
    68941 gataatcggc gggggcatgg cttacacgtt tgtcaaagca aacggcggca aaatcggaaa
    69001 ttcaatctgc gaagacgacc agcttgaagt cgcaaaagaa gtagagaaaa aagctgccgc
    69061 aaaaggcgtt aaaataatta tggcaaaaga tgttcttgta acagacgatt tttcaggaaa
    69121 cggtacaaac aaaatcgtag atgtttatga aatccccgac ggctttgaag gagttgacgc
    69181 aggccctgaa actcaaaaag cgtttgaagc tgtaatcaaa acctcaaaaa caattctttg
    69241 gaacgggcct gtaggtgcgt ttgaaaacga caaatttact caaggcacaa aagctgttgc
    69301 acttgcggtt caagaagcca caaaaaacgg cgcggtaagc gttcttggag gcggcgatac
    69361 cgttgcggcg gttaaaaaat acaaattgga tgaaaatact ttcacccaca tttcaacagg
    69421 cggcggcgca agccttgaat ttatcgaagg caaagtgctt cctggcgttg acgctttaga
    69481 tgataaataa catgattgaa ttactggcac ctgctaaaga taaaatatgt gctaaagcag
    69541 caatagattt cggtgcagat gcaatataca taggtgcaag cgaattcggg gcaagaaaaa
    69601 acgccccgaa ttcgcttgct gatattgaag aaattgttca atatgcccat aaatttaatg
    69661 taaaagttta tgctgcggta aacacaattt taaccgatga agaaattaag agggcagaaa
    69721 aactaattga agatttatca aaaatcggca ttgacgcgat aataatacag gatatgggct
    69781 tactttcagc aataaaagcc aataatattt gctgccatgc ctcaacccaa tgcgataata
    69841 caacggccga aaaagttaaa tttcttgaaa gtatagggtt ttcaagagta attttggcgc
    69901 gggaactctc gattgaacaa attagagaaa taaaaaagca aaccaatctt gaactggaag
    69961 cctttatcca tggtgcatta tgtgtttcat acagcggaaa atgttaccta agttatgcca
    70021 taggcggacg ctcggcaaac aagggcgagt gtgcccagcc ttgcagaaaa aaatattcac
    70081 tcattgacga aaaaggaaat tacctcgcaa aagacagata tttgcttaat ttaaaagatt
    70141 ttaacgcctc aaattacata aaagaattag ctgaagcagg cgttacatca tttaaaattg
    70201 aaggaagatt aaaagacgaa aattacgtaa aaaatgtcgt cgcatattac agaaaactaa
    70261 tcgacgattt agattaccaa aaaacatcgt ctggtaaaat attatttgat tttgaacccg
    70321 atttgaataa aagttttaac agaggcttta cggattattt tctcgaaggc aaaagaaaaa
    70381 aatgctttga ctttgacagc ccaaaatcaa aaggtgaata catcggagag gttacgaaaa
    70441 tcaaaaaaga taactttgaa atcacgtcac ccctcaccct tcacccttca catctttttt
    70501 cccaagacgg cttatgtttt atgtacaacg gcgaaattca gggctgtctt gtaaacaagg
    70561 ttgaaaataa caaaatattt cccaacaaaa tgaatggggt taaaatcggg acaaaaattt
    70621 atcgaaattt ggattctgaa tttgaaaaaa aactaaaaaa ttcaaaaaca caaagaaaaa
    70681 taggggttaa tttaactttt gaatcagata aaatcaccgc agttgatgag gacggaaata
    70741 ttaccaaaat taattatgct tatgaagaac ttgctaaaaa cagcataaaa gcaaaagaaa
    70801 caataattaa acagcttaaa aaatcgggag atagtgattt tgaagttgaa gatgtaacta
    70861 taaacattaa caagatgccc tttttgcctg tttcaaaact aaacgaactt cgccgcgaat
    70921 tgcttgaaaa attgatgaaa aaacgcctac aaaactattc ccctatacat catcctgaac
    70981 caatttcaga atctcatcaa cccttaaatc aaacaatcaa ttatcaaggc aatattttaa
    71041 acaaaaaagc aaatattttt tacgaaaaac acggatgcaa aattgttgaa tccgcacttg
    71101 aaagtggtaa aattcctgtt tgcggtaaaa tagttatgac cacaaaacat tgtctgaaat
    71161 cggcttttaa tatgtgcaaa tcaagtgaaa agatgttttt ggttgatgaa aagggcaaaa
    71221 aatattcgct aaaatttaat tgcgataaat gtgagatgga agttattttt taaaaaaggt
    71281 taatcaatgt catatagggg aaaaaaatta ctaaaaataa tgcaaaatgc taataattac
    71341 aataattttg taattaattt aattttaaaa tcaatcgttg ataaaaactt taaaaccctt
    71401 gattttggag caggatatgg tttttttgct aatcttttaa gaaaaagcgg ggttagtaat
    71461 atcaaatgtg tcgaaataga cgaagaatta agcgaacatt gcaaaaatct tgattttttt
    71521 gtgtttaaga atctgcatga catagaaaat aactcttatg attttattta tacccttaat
    71581 gttcttgagc atatcagaga tgacaaagcg gttttacttg cactaaaagc aaaattgaaa
    71641 aaagaaggga aaatttttat ttatgttcct gcttttaaat tattatattc atcctttgac
    71701 aaaaaaattc gccactgcag aaggtacaaa aaagaagatt tgattccgtt tttagaaaac
    71761 aacggatttg aaattttaaa tgcaaaatat gttgacagtt ttgggttttt cttagctcta
    71821 atatacaaat ttataaacaa aaacgacggt gaaattaata tgttaagcgt taaaatattt
    71881 gataaaattt tattccctgt cggaagaatt tttgacaaaa ttatctttga aaaattttta
    71941 gggaaaaacc ttattgtaga agcaaaactt attcaataat ttttataata agttctttgg
    72001 cggtttttga ggcttgctca aaaaccgctt tatttgtcca aaaatcatcg cgttcaatat
    72061 atttttttac aatttttctt gcgtaagagc cgacggaaat tccgttgaaa tcaattccga
    72121 aaatttttgc aagctctgcg gtcttagaat ttgttccgcc tgaaattaaa agataaacag
    72181 gcagtttttc gttagctata atttctgcca tggcaactgc aggaagtgtt gttttaaaat
    72241 catcttcgcc tccggacatc gggtttccgt ctgcctgaat tattgttgta taaagttttc
    72301 tgttttttat taaattttta atcctgttta aaactgcttc atttgatagt tttgacctgt
    72361 caatacaaat actcagcatt ccgtcaaagt ttttgtttaa ataattccat ctttcctgaa
    72421 catctttttc atctgtgccg gaagcatgca attcaataca ggtgaagggt gaagggtgaa
    72481 gggtgaagag aggaaaaaat atgtcttcta tcggcttatt tttttgatat atttcaatag
    72541 cactatgttt acaggttttt aaacatcttg cacagccgat acatttttct tcgataacca
    72601 taaaatcagg gttgatggct ttttgcaggc aggaatcaat gcaattgaca cattttttgc
    72661 aaaatttttg attgattttt gctttattaa aatgcggctc gccttttatt ccgacactta
    72721 tgcaaaaatg gtaatcagat aagttttttt tgtccgtaac ccttaggatg cctttttgtg
    72781 cagcctcaat aacttcttta tgcggagata ggtcaaaaaa tctgcagccg gatgcagcat
    72841 aaattgcaac aagtttttcg acttcgtcta aatcctcgtt tcccgccccg caaattaact
    72901 tgaaacattt tctgctttct aaaagttttt gcaatccgca ttgtcgggat tgcaatcccg
    72961 acctacctct cacccattta ccccctcacc tctcacttct ccccaattta tcccaattct
    73021 tgtgctacac gggcaacata ttcataatat tcgtttttct tatattttgc cgcttgttca
    73081 aggaggtctt cttttttcaa aaacttcatt cttagcgcaa cttcttcaag acatgcgatt
    73141 ttgacaccct ggttttcttc tattgtctga acatactggc ttgcctgcaa aagcgaagag
    73201 tgagttcctg tatcaagcca tgcaaagcct ctgcctaaaa gttcaacgct caaatttcca
    73261 tttttaagat aaacattgtt taaatcggta atttctaatt cgcctcttgc agaaggctta
    73321 agatttttag catattctac aactttgttg tcataaaaat aaagtccggt aaccgcataa
    73381 tttgatttag gattagcggg tttttcttca atggaaagtg ctttgccgcc tttatcaaaa
    73441 tcaaccaccc cgaatctttg gggatctttt acctgatacc caaaaaccgt tgctccacct
    73501 tgagacttga ttcttcttac cacgccttca agcatgcttg aaaacccctg cccgaagaag
    73561 atattatccc ctaaaacaag tgcagctgtg tcatttccta taaattcttc acccaaaata
    73621 aaagcctgcg caagcccgtc gggacttggc tgttccttat aagaaaactc taccccgaat
    73681 tgttttccgt ctcccaaaag ttttttaaaa ttgggcaaat cctgcggggt tgaaattatc
    73741 aaaatttctt ttattcctgc aagcattaat accgaaatcg gatgataaat catgggttta
    73801 tcataaatcg gcaacagctg ttttgaaaca accattgttg taggaaacaa tcttgttccg
    73861 gctccgccgg cgagtactat tcctttcatt ttaacttcct ttcaaggctg cttttttatc
    73921 ttctattgaa tgcatccatg cctgattttt caaataccag tcgattgtta atttaattcc
    73981 ttcctcaaag gtcaaagaag gttcccagcc cagttctgtt tggatttttt cattatcgat
    74041 agcatatctt ttatcgtgtc ccagcctgtc ttcgacatat ttaatagaac tttcgtcttt
    74101 acccatggca tcaagaatga tttttgtaat ttccatattg gttttttcat tgtgtccgcc
    74161 gatattgtaa acttcaccaa gtctgccctt gtgcaaaaca gtatcgatag ccgaacaatg
    74221 gtcataaacg taaagccaat cgcggacatt caagccgtct ccatagacag ggactttatc
    74281 cccttttaaa agctgtgaaa taaagaaagg aataagtttt tcaggatact ggtaaggtcc
    74341 atagttattt gaacatcttg ttgtcaaaac aggaagttta taagtttccc aataagccct
    74401 cacaagcatg tccgcacttg cttttgaagc cgaatacggc gaatttggtg caagcggcgt
    74461 tgtttcataa aaataaccgg ttttgcccaa tgttccgtaa acttcgtctg ttgaaacctg
    74521 caaatatctt tgaattccta attctttgca tgcctgaagc aaattcagcg tacctttaac
    74581 attggtttct ataaaaatct cgggtcctgt tatacttctg tccacgtgac tttctgctgc
    74641 aaaattaaca acgtaatcga ctttttcaac caactcgcga acaagcgtcc tgtcacaaat
    74701 gtcaccttta acaaaagtat aattcagatt atctttaaca tcgtcaagat tttctatatt
    74761 gcctgcatat gtaagtgcat caagatttat tactttataa tcgggatatt tatttaaaat
    74821 gtgcctcaca aaacagcttc cgataaatcc ggccccaccc gttacaagca atcttttcat
    74881 agcaattcct ctttatttaa ttcttttaat ttaggctgaa tcttatcttt ttcgctcaaa
    74941 atcggctcaa agtcaattcc ccaatcaatt cctatttctt catcattcca caaaagccct
    75001 ctgtcacatt ctggacaaaa ttctgcatcc gttttgtata aaagttctgc ttcatccgat
    75061 aagacaacaa atccgtgagc aaagcctgcc ggaacaaaaa gcatgttttt attttcttct
    75121 gaaagttcgg ccttaaacca cttaccgaat gtttttgaat tttttctcaa atccacaata
    75181 acatcaaaaa cttttccttt tgaacatctt ataagttttg cctgcggctt tggagttgcc
    75241 tgataatgaa gtcccctcaa tacacccttg gaagattttg aatgattatc ctgaataaaa
    75301 tcgcaagtta tgccattttg aaaaaattcg gattttctgt aagtttccat aaaaaacccg
    75361 cggttatcac caaaaacctt tggtttaacc aagataacgc cttctatttc ttgtttttca
    75421 aattcaaacg gcattaaatc ccctatcatc aatcattaca aaagcgattg cctttgtttt
    75481 atcatgcgac aaactgactt gtattgtcaa tttattatca agattgttta acaatcttat
    75541 ttttggaaaa ttattttcaa tgtgataaac ttctatcata tttaaaggcg gatgagaaat
    75601 atttaatcct gtaagtgctt ttaaaacagc ttctttagcg cagaatctta cggcaaaatg
    75661 ctttgcaggc agttttttgg ataaacaata ctcctgttcg gattttgtgt aaaccctgtc
    75721 caaaaaatcc tgcgatttat cctgaaatct tcttatatct tcaatatcaa cgccaatact
    75781 cataaattaa taataataaa aaccctatcg aaaaacaata gggcttttgc ttttattaaa
    75841 ttaggttaat gtttatcctc tcagcttttt tgatttacca aactatgtac tattttgctg
    75901 aatttttctg catctaagca cgctccgccg acaagaccgc catcaatgtt gctttgagca
    75961 aataatttgt ctgcattgct atcatttaca cttccgccgt ataaaattga aacatctgcc
    76021 gcgttttgtt cgccatatat ttttgcaatt tcatttcgta tcattcgaca cattctgtca
    76081 gcttcatcgg catcacaggt ttttcctgta ttaattgccc aaataggttc gtatgctata
    76141 acaacttttg ttatatcttc ttctttaata ttttttaatg cttttataac ctgtgtagaa
    76201 accacatcat cggcatcgcc tgcttctctt tgttttaaag attcacccac gcacacaaca
    76261 ggcattaacc ctttttcaag tgcggaaagt gtttttgcgt taacttgttt atcggtttct
    76321 gcaaatttgc ctcttctttc ggagtgccct attatgacaa aatccgctcc tgcttctttg
    76381 tacatatcct gagatatagc ccctgtaaca gctccgcctt tttctatgcc aagatcggct
    76441 tcctgcccgc ccaatttgat atttgccata tctacataat ttctaccctc tttaacttta
    76501 caaaaagcca acgccgttgg agcaaagcct acataaatat cgttatgagt aggcaaatcc
    76561 aacatttcaa aagcacttct gttaataagt tctgatgttt ctttcgggct tttgttcatt
    76621 ttcatatttc ctattaccat aggtttacca gtgaaattca tatttgtttg tattgaatta
    76681 attctcatct tttatccttt cttctgaaat acaaagcaaa gatataaaat atctttgctt
    76741 tgtataaata atttatgctt ttatatcaag attttttgcc aattcgacag ctttggttgc
    76801 tttatctgtt gcatccattg cctgacctga agaatttaac atatcggttt ttcttgcgcc
    76861 tattgtttca agagttttca taataggttt tccgtaagtt ctggggtcaa tagatgcttt
    76921 atctgtaacg tcaagttctc ctgcgacatt tttgaacgtt ccaacatagc ccagtctaaa
    76981 atcagtatct acgttaactt ttgaaatacc tctaagcgtt gcttctttat acatttcttc
    77041 gggaacacct tttccaaaat aattagcagt atttgatttc attgcctcta ttgcgcttaa
    77101 aacagtcaaa gcacctgctc tggaattaac taaatcatta atactgtttt ctaaggtttc
    77161 aaatttttta cggggaataa ccacgctttg attaaacatg tcaatcaaat cttttggggc
    77221 acttgacgca ccgtgtaata caaaaggata ttgtctccat cctgcatcac cgggcttttt
    77281 acctatgttt tcgggcaata ttgcttcgac cgctttttta atttcagata acctttcgaa
    77341 atctaattgc ggtttttcgt caggacggaa tttaaccgca ccgtgggatg ttccgattga
    77401 aattgcaagc gaatcacaac cggtttcttt tacaaacctt gccgcctctt gagggtctgt
    77461 aaatttagaa gttttatttg aaacttcatc ttcaacacct gcaagttttc ccaattctgc
    77521 ttctacaaaa ataccttttg aatgtgcata atcaacaact tcttttgtta atctgatatt
    77581 ttcatccaac ggaaattttg aaccgtcaat cataacagaa tcaaatccgc cgtcaataca
    77641 ctccttacat acttggaatg tgtcgccatg gtcaaggtga agcattacgg gaataccttt
    77701 tgcctcttct tttgcaactt ttaccatttg aaccaaataa ggaagccccg cataatctct
    77761 tgcaccttta gatgcttgaa taattaaagg ggattgtgtt ctttttaccg cattaattat
    77821 cccgtggaca tttaaaagat cgttcacatt aaacgccggt atagcataac ctttttctaa
    77881 agcatgctga taatatgctc ttgtctgtgc aagtttttgt gccggtgata actctgtatt
    77941 attgccaaaa tttacttttt gaacatttgt tctttgtacc aatgcaatat ttttgtttgc
    78001 ataaaacccc tgtgtttgta aattaaccct cattattttt aactccttaa actgtttaca
    78061 acatgcttta aaaagcgtaa aaatattttt tgctaccaaa ttttaaaaaa attacatttc
    78121 ttaataacaa tagggcggcg gattttttaa aaaatccgcc gccctatatt atttcaataa
    78181 aattacaact ccgccaactg ttgtcttaaa tcaattattt gctgtgtgta atattcaagc
    78241 caggtttgtt ctgttgtttc atctttaact tcgggctctg cgattgccct tattcttttt
    78301 atatcaaact cttctatttg agttttcaaa ttttcgatat cggcttctct ttgttcttga
    78361 attcttgcct gagcatattc ttgagtgtga gaaatatcag ttaatattcc gtcaatgact
    78421 ctatattttt cgggctcaaa ggtcgcatag tcaatatcta cataaacata atccaaataa
    78481 ggattttcgc accagcttaa taatttattt tcttcaattt ctacgtacat ttttatacct
    78541 ttctttatta ttttttgtaa aattacaagt tatatcgcta agagcttatg ttcccttcgt
    78601 tgggaaaaat ttaaggtgtt tccacgaact tccgtcatat tcataattaa gtttataata
    78661 gataccctta ggaataggaa taaaacccca tgccccataa ccttgccctg agtgattaaa
    78721 caaaagatta cttgtccaat ttgttccgtc aaagctgtag gaaaatgtta atccgaaacc
    78781 gcaacctacc ataacaaatc cgtcagattc ggcaagataa gaagtgttcc aactctttga
    78841 taccccgctt gaataatttg gcatcggcaa attagttatg gattttgcca aatttgtaga
    78901 agcctttatc gctgaattta aagttatatc atagtcattc tgattgtaag ggaaagtttc
    78961 aaccgcggtg atgactccgc ctgcaattgt tactttaccc aaaggaatat ccaaaaattc
    79021 cacatccgtg ccgcttgtat attttattgc tttaagaggt tcaacacttg tatttagcca
    79081 aatatcccct tcaaccaatg tcggacgggt tgcctgcttg taaatattat tagcaagcga
    79141 atatacgttt ccgctcgttg aaataaaaac attatgcgcc ccgttaggct ttccgctcat
    79201 actcaatgtc cctacagaag taaaagttgc aaaagttcct cttgcgttag aaattattaa
    79261 gctcggataa gaacctccga ctttaaaaga cactgacata cttgaataac tcaataaatc
    79321 accggctccg tttgtaacat ttccggattt tacacaaaat cttgtttcgg ctgcgttgat
    79381 tttagaattc aagtccgttg acaaagaatc caactgcgat ttattgattg catttgtaga
    79441 tgttgtagca gcagctacat taaaagcttg tgtagaattt cctgctttat ctgctttttc
    79501 cgaaagagca atgctcaccg cctgaaaatt ttcattgatt tcctgtgctt ttgcttttgt
    79561 tccgggcaca aattctatta acgataaata tcccgacata ttttctcctt tcttttttac
    79621 cctagcatta tgaaagattt ttgaaacaaa aaaaagtccg caaaagtgct tccgtacaat
    79681 aacgtaagta tttttacgga cagctttaaa tttgcatttt tataaaaaat gaagtatcat
    79741 ttttttatga aaatatctgt tatagttccg gcttacaata caagcagcta ccttaaacag
    79801 tgtcttgaaa gtttaatttc acaaactttt aatgacattg aaataatttg cgtaaatgac
    79861 ggctcaaccg ataattcgct tgaaatttta aacaagtttg ccaaaaaaga caaaaggata
    79921 aaaattattt ctcaaaaaaa tcaaggattg tctgccgcaa gaaacacggg aataaaggcg
    79981 gcaaaaggtg aatatatagg ttttgtcgat tcagacgact gggctgatga gtcgatgttt
    80041 gaaaaacttt atcaaaacgc caaaaaattt gatgccgata tttcaatgtg ttctataacc
    80101 acattcaatg aggatacggg cgtttataat aattccgacc cttatatgac aatgaattta
    80161 tttcctgaaa gctttgaaga cagagtcttt agccctaaag aaacctatga ctttattttc
    80221 agaatctgtg tagttgcgtg gaacaaaatt tataagagag attttcttga taaaatcgga
    80281 ggtttcttca aagaaggttt ggtctttgaa gataatttat ttttctgtca aacctttatg
    80341 caagcaaata aaatttgcat aacaaaagaa aatctgattt tttacagact aaattcgcca
    80401 aaatcaataa ctttcgggga taaagatttt caaaaattgg atttttttga ggtttttgaa
    80461 cttatagaaa aatttttgaa agaaaaaaat tactacggcc aacttgaaga ttattttcaa
    80521 aattacaaaa aaggcacact tgtttactgg tacaaaaaat taaaaaatga agacacaaaa
    80581 caaaagtatc ttaaaaagtt taaaaaatta tatcctgaag aaagcttgat actaaaatga
    80641 ttgtcctaac ggattaaaaa aaagtccgtt tttaacgaca tcttcataaa gctcttcatc
    80701 tttttccaac tctgccatag tcgtgggatt aaggtcaata aaaacatcca attccgtctc
    80761 gactttgcca acaataggcc atattaactc gcgctttgct ttatcttcag aatcaaaaac
    80821 agctacaagt tcaatgtctt cttcttcgtg aaaatttccg tcaagaaaca acccgtaaag
    80881 ataaattcca tggaaatcat caaaacgctt gctgaattca gcaattaaaa gattaacagc
    80941 tttttcgacc ttttcatgca taggggaaat tataatactt ttagccaatt ttgtcaatca
    81001 aatcggcatc tgtttttacc gaatatttta ataattcatt ttccagcctt cttctataac
    81061 acgtgctgta caaaaatcat cttgaaaatt ttgtccgcaa ctccattccc aatcttcacc
    81121 aatttttgtt gaaccccagg gaacaagcaa tggcccatta ccgtttgtta tataaaaaga
    81181 gaaaaaatcc cttccgtacc tatttggagg tttcattcca tttatatcaa cttgagctac
    81241 accacaaagc tgtaagttta aatttattac cccaccaaac atactttgtc catgccaagc
    81301 ctctatgcaa tctccagcta cagaaacagc ccaactcatc ccgtcaagag ttataaattt
    81361 atatacataa ggacttccca accaattata agcctcatca ttagtatata aattaaagta
    81421 aattttggct gacccgcaat cttcatcttc cgttccacaa ttttttgaaa gcttaaaata
    81481 ctttacaatt tcttcaccca aagccttatt tttaagttcc cattgttctt atggcgtgat
    81541 attatttcta taagtttcta tatcaatacc atccgggggc aatcctaagg caaacagccc
    81601 tgtacacttc aagtcacccg ggcaccccat gtcattagac atcatagcca aagcttgatt
    81661 aaaagtagta taagcttttt taagagctat aacatattgc tctttttgaa aattggcaat
    81721 caaacttggt agggtcaagt ccgcaatcat accaattatg cccaaaacta tcaaaacctc
    81781 tgccagagta aaagctattt taatccttaa accttttgtg tccaaataac ctccgagact
    81841 aaaagacact tgtattttag ttttattaac taaaaaagtc aaattgtaaa caatttattt
    81901 taaaagattt ataagcttta atcttgaaac agatatctgt tctgatttag caaggttttt
    81961 aaccgaaatt ttgttatttt taatctcgtc ttcacccaaa atcaaagcat aaagtgccga
    82021 ttttgaggct ttttccaact gtttaccgaa ttttttactt gtcatatcaa aatcggcact
    82081 gatattattt tctctcaatt cctgaaccaa tttcaaagcc tcttgggtag aatccgaaac
    82141 cacataagcc ttaagctttt caggctcaag ttcaggcaaa agtgagttta atctttccat
    82201 tcccattgcc caacccacag caggagtgtc ttctccgccg agatttttca caagactgtc
    82261 gtatcttccg ccgccgcaaa cggcattttg tgagcctaag ttgtttgatt taatttcaaa
    82321 aacggttcta ttataataat caagccctcg tacaagtaat ttattgacag aatacttgat
    82381 ttttaaaaca tccaaatatt ttttcaaagc cgaaaaatgc tcgctgcagc tttcacaaat
    82441 ataatcagaa gttataacct tttgaatttc agatttttta aatatttttt gacagctttc
    82501 gactttgcag tctaaaagtc ttagcggatt tttttcgtaa cgtgcctgac aatcagggca
    82561 aagctctttt aaataaggtt taatagcccc ttttattgaa gctttataaa tttctctaca
    82621 atcgtgacag cccaaagaat taatctcaac atcgagattt tttaatccca gttttttaag
    82681 ataatcaacc gcaagcataa taacttcggc atcggctgaa ggctcttgaa taccgaacat
    82741 ttcaaccccg acctggtgga attgcctttg cctaccggct tgcggacgct catacctgaa
    82801 catcgggcct ttataccaaa gctttaccgg cgtgggaaat ctatgcatac cattttctat
    82861 atacgctctt acaacccctg cggtattttc gggacgaagg gtcagggaac gctcgctttt
    82921 ttcaaaagtg tacatttctt tgttgacaat atctgtagta tcaccaactc cgcgcgagaa
    82981 taattcagtc gcctcaaaaa tcggcgttct aatttcccta taacccgcat ttgaaaaaac
    83041 atcaagcgcg gctttttcaa gacactgcca attaacgatt tctgagggca aaatatcctt
    83101 tgtgcccttt tgtgctttga ttaaattagt cataaagcga ttatattaag ttaagtgtca
    83161 aaagtcaaat taggaacaaa aaaataacaa aaagggctgg attttccttc ggaaaatcca
    83221 gcccttttat ctattcacta tttactgttt cctatttact ctttcgtata ttccgcatct
    83281 atgacatcat catcagatga agatgcttca ctttgagctg aagcttcgcc ctcggaagat
    83341 gtgttttgag ctgtttcacc ttctttttga acctgctcgt aagccttttg agaaatcgca
    83401 aacattgttt gctgcaaatc ttcagataat tttttaactt cttcggttgc tttattttca
    83461 tccaaagctt tttgaagtgc ttctttctgt tcgtcaagtt ttgttttatc ggcttcatca
    83521 attttacctt caaagtcttt gactattctt tcagcggaag ctataagccc ggaagcatta
    83581 tttcttgttt cggcttcttc ttttttggct ttatcttctg ctgcgttggc ttctgcttct
    83641 ttaaccattt tatcaatgtc atcttctgac aaattgcttg aatttgtaat tgtaattttt
    83701 tgttctttat ttgtcgcttt gtctttagct gaaacattta caatcccgtt tgcatcaatg
    83761 tcaaaagtaa cttcgatttg aggaagccct ctcattgcag gcggaattcc gtcgagtctg
    83821 aacataccca aagatttgtt gtcttttgcc ataggtcttt caccctgcaa aacgtttatg
    83881 tcaactgctg tctgattatt ttccgctgtt gaaaacacct gtgattttga aacaggaatc
    83941 gtggtgtttc ttggaaccaa aggagttgaa actccaccta gagtttcaat acccagcgtc
    84001 aaaggggtaa catccaaaag aacaatatct ttaacttcgc ccgcaaggac acctgcctga
    84061 atagctgcac ctactgctac aacttcatca gggttaacag attggttagg gtctttgcct
    84121 gtgtattctt taacaagtgc ctgaactgca ggaatacgtg ttgatccgcc gaccaaaacg
    84181 acttcgtcga tttcgccttt tgaaagattt gcatctttaa ttgcattttc aacaggtttt
    84241 ttgcatctgt ccaacaaatc gtgtgtcaaa tcctcaaatt ttgctcttgt caaagaaata
    84301 tccaagtgtt taggaccgtt agcgtctgcc gttatgaaag gcaggttaat atttgtttca
    84361 acaacggaag aaagttcgca ttttgctttt tctgcagctt ctttaatacg ctgcattgcc
    84421 tgtttgtcgt ttcttaagtc tataccttct gatttcttaa actcatcaca aagccaatcc
    84481 ataactttat tatcaaaatc gtcaccgcca agatgagtat cgcctgatgt cgaaagaact
    84541 tcatgtacgc catctcctat ttccaatatt gatacatcaa atgttccgcc tccaaggtca
    84601 aataccaaga ccttttcggg tttaacttta tccaaaccgt aagccaaagc ggctgctgtc
    84661 ggttcgttaa ctatacgaag aacatccaaa cccgcgattt tacctgcatc tttggttgcc
    84721 tgtctttgtg catcattaaa gtatgcagga acagtaatta ctgcagatgt aactttttcg
    84781 cccaaatatg aacttgcatc atcggcaagt tttcttaaaa tcatcgcgga aatttcctgc
    84841 ggagtgtagt ttttatcgtc aatttttact ttgtaatctt ctcccatatg cctttttatt
    84901 gaagcaactg ttttatcggg attcaaaatc gcctgacgct ttgcaagctg cccgacaagc
    84961 ctttcgcccg tttttgaaaa cccaacgata gaaggagttg ttcttgcacc ttctgcattt
    85021 gcaataaccg tgggcttacc gccttccatt accgctacta ccgagtttgt tgttcctaaa
    85081 tcaataccta ttgtcttagc cattttttta cgctccttgt atatattagt atgtattctt
    85141 agagtgaata gtaaatagta aatggatttt taatttctat tcactattta ttatttccta
    85201 tttacttctc taaatttaac atcgttttta aaagatcatt aaaaaataaa caaaaaatta
    85261 atcttttaac ttgtaaaatt ttattaagca attagcaaaa aaaattttta ggatttttca
    85321 aacattcata cacgtataaa aggaggaaat atgaaaatat acgcaattaa caattacgca
    85381 aacacaaaat taaattcaac aaatcaaaat ccaatcggtc tttatagtta cggactaaaa
    85441 gcagacagtg taaactttac ctgctcacat tgtgaagaca aaaaaacaca acgctcgatg
    85501 cctgttaaaa aagatgtccg cgacttggga aacattgaag gcaaaacagt tttaatgaga
    85561 gcagatttca acgtttcaat gaacaaagat ggaagcataa atgattcttt cagaattaag
    85621 caagcggtta aaactattaa atatttaaca gacaaaggcg caaaagtcgt cttaatgtca
    85681 cactttggcg atccggctaa agaattgaaa aaagcaaagg caaaaaatcc cgacttaaac
    85741 gaaacagaag aaatggctaa aattcaacaa aaactaagcc taagacccgt tgcagagaaa
    85801 ttatccgaat ttttaggcaa agaagtttat ttttactcct atcgcaccga ccatcttcca
    85861 tacaacagct tgcaaaatgg tgaagtgtgt cttatagaaa atacaagatt taatgatggc
    85921 gaaacaaaaa acgacccggc tttagcaaga cactggggct ttaatacgga catttatgta
    85981 aatgatgcct ttggagcagc acacagggca cactcttcaa cggaaggagt tgcacaatat
    86041 tgtaaaaaac ttgccaaccc cgctgttgca gggcttttga tggctaatga agttgatatg
    86101 gcatgcgaaa tattagacaa tcctgagcgt ccatttactg tggtttctgc cggctcaaaa
    86161 cttgaatcaa aaataggacc tataaataca ttgattgata aaatgcagcc tgaggacaac
    86221 caaattatcg caggattaat ggttatcccg tttattatca atgaaaatcc gaattctcct
    86281 ttgagaaaac tttataaaaa tgaagaagaa ttaaatgctg acttggaaag agcaaaatca
    86341 atagttaaca aatgtgcaga aaatggcgtt aatttaataa tgtcacaaga tgttgttgtc
    86401 caaaacaaag atacaaaaga agttaaaaca gttttcgttg aagatattga agacggctgg
    86461 gaaattatgg acgtaggaga aacaacaata gaaactctta aagacaccat atcaaattcc
    86521 aaaactgttt ttgtaaacgg acctgtagga tactttgaag gcggttttac aaacggcaca
    86581 aaagaaatgc ttcaagctat ccaagacctg aaaaagaata accctgaagc aaaaaccatt
    86641 gcaggaggcg gtgatacagt tactgctatt acaaacatct ttggtgaaga atcatttgaa
    86701 aatttcaccc acgtttcaac aggcggcggc gcactgcttg acttcttaag cgggaaactc
    86761 cccggatatc tggctttaga agataaataa aaatgaatta aataaacact gaggggcggc
    86821 acttttcaag tgccgcccct cagtgtttat actttttaaa tttaaaaact tttgcaataa
    86881 agctctatgt cctcatcgga gttcatttca gtcactgcaa ttaagattct tttttcatca
    86941 agcttcagtc cgcctattat accgttttct ctaagtttta acagaaattt atcagaattt
    87001 tcgacttcaa ttacaaattc gttgtaaaaa ttttcattta tagtcttaat tcccttttga
    87061 gaaagctttt ccgatagttt gtgagcattt tgtgccgata aaaatcctgc ctgcttaaag
    87121 cctttttcgc ctaaaagggt caaatataaa gtggcacaaa gtgctattag tgcttgattt
    87181 gaacaaatat tacttgtggc tttttccctt ttgatgtgct gttctcttgt ctgaatggta
    87241 agcgtaaaag cctgatttcc ttcagcatca agggttctgc cggctattct tccgggcaat
    87301 tgtcgcatga atttttcgcg acaagccata aatcctgcat gcggacctcc aaacgacatc
    87361 ggaatcccaa gcggctgaat atcacctact actatagcag caccgtattc tgcaggagct
    87421 tttaaaatac tcaaagacga aatatcgaca caaaccgcca aaagtgtatt tgccaaagtc
    87481 aaaacttcac ctaaattaat atcttcaatt tcaccgtaaa aattcgggtt ttggattaaa
    87541 acacaggcaa attcacctgt attaattttt tcttttgtga ttttccactc aatttcaatt
    87601 ccgatagccc tgcaataggt ttttattact tctttgtact caggattaat gttttcatca
    87661 attaaaactt tttcgcgttt tgtaattctt gatgccatta aaatggcttc agcacaagct
    87721 gtcgccccat catagacaga agcgtttgcc acatccatgc cagtcaaatt gcaaatcatc
    87781 gattgaaatt cgtacataac ctgaagcgtt ccttgagaaa tctccggctg ataaggcgtg
    87841 tatgcggtaa gaaactcaaa tctttgagct acctgagaaa cgcaagcggg tatgaattta
    87901 ttataaactc ccgcacccaa aaaacttatc atttcagtat tgtttttctt tgcaagtgct
    87961 ttaacttgtt tttgagtttg catttcactt aaggcgtcag gcaggtcaaa ttcagcacaa
    88021 agtgcttgtt ggggaataat tttaaataag tcgttaaccg atgatacaga gatttctttt
    88081 aacaattgtt ctcttgtttc atctgtatgt gccaaaaagt tttccactat gccacttctt
    88141 ccctgtaatc gttatattcc agcaaatcaa cagagtcctg tgtcgcattt tgactttgaa
    88201 ttttaaccaa ccagcctttt tcaaaagggc tctcgttcaa aatttcagga ctgtcaacca
    88261 ctttgtcatt aatttcaaca attttgcctg aaatcggcat gtaaatttca gatgccgcct
    88321 ttacagattc aatagtggca aaaacttcgc ctttaacaaa ttctgctccg acatcgggaa
    88381 gctccaaaaa aactatatcc ccgagctgtt caacggcata atcggtaagc ccgattacat
    88441 atttgtcccc ttgctccaaa atatactcat gggttctgct gcataaaatt ccttctgtta
    88501 aactcatctt taattattct ccttataact aaatcttatt cttcttcaaa ataaaaggtc
    88561 ttttgaccac ttcagcattg tataatttat ctcttatcat aacctgaaga acggttcctg
    88621 tgcaaatctc tgtaatattt ttaacataag caagtgcgat gttctcgttt aacacaggcg
    88681 aaacaccacc gctggttata attccgacat tctcattatt gtaaaaaacc tcatacccgt
    88741 gacgggcgat tgccctgtca agcattttta gcccgacaag ttttttatct ccgcctgttt
    88801 gaagatgttt ttgtatggtt tctttcccgt tgtaatcatc gattttagtt ttggaaacag
    88861 accatgtcaa atttgcttca atcggagaag tttgttcatc catatcattt ccgtacaaat
    88921 gaagagcggc ttcaagcctg agagtatcgc gcgcacccaa ccctataggt tttaacccga
    88981 attcttttcc cgcctcaatt atataattcc acaaaagttc ggaaaattcg tttttaacaa
    89041 gaatttcaaa cccgtcttct cctgtgtagc ctgtccttga aacaaaaagg tttatattaa
    89101 aaagttctgc tctttttata gagaaaaatt caggctgatt ttcttttttt aatcccattt
    89161 tatccattaa atccgaagct ttcggtcctt gaaccgccaa cagagagtaa ttgtgggatt
    89221 gattgtctat tgaaacatta aacccgagtg aatttctgac catccaattt aaatcttcgt
    89281 ctattcttga ggcgtttaca attagcagat aatcattctc ttcaagttta taaataatca
    89341 aatcgtctat aatcccgcct tctttattta aaagctggca gtaaacagcc tttaaatttg
    89401 tcaatcttga aacatcttgc ggaacaagtt tatttaaaaa aggcagggca tcagaacctt
    89461 ttacaaaaac ttcacccata tgcgaaacat caaaaagtcc cacattttct cttacagttt
    89521 tgtgttcatc tataatcgaa gaataagaaa caggcatcat ccaacctgca aattcaatca
    89581 tttttgctcc cagcttaacc tgttcatcgt gtaaaaaagt ttttttagtc attttttctc
    89641 ctcaatgtct tattttaaat tttgctctca actaaaaatt atgtcaagtg tcttggtatg
    89701 aggctgcgga aaaattgaaa atttttctcg cacctccata gtccagtaaa agccaaacta
    89761 tcctttaacc aaggatatgc ttggctttta ccttcttaag gtcggacacg gaaaattctc
    89821 gaaattgtag atttcggaat tttccgtgtc ctctatgttt tttattgcca ttttattgaa
    89881 aaaatgttat cataattaaa aaaatagtgg gagatttaaa gttggataaa tttgaaataa
    89941 acgattttct taaaaaatcg gttttaatgg gtgcctctga cgttcacctg aaagttgatg
    90001 aacatccggt tataagaaaa gacgggcgaa ttataaaaat agatatgcct aaattaaatg
    90061 atgaaaacat tgaaaacatt ataaatgttc ttgcgccaaa aaccataaaa aacaaaataa
    90121 atactgtttt tgacatcgat ttttcatacg aacttcccgg tatttcaaga tttagggtaa
    90181 atttaagtca acaattgggc aaaagtgctc ttgttataag aaatatccct tacaaaatca
    90241 agagtcttga agaattaaac ctgccggttt caatggaaca atttgcgcag tataacaacg
    90301 gacttgtaat tgtaacaggc cccacaggct gcggaaaatc aacaactctg gcatgcttaa
    90361 tagactatat aaatacgaat tatccgaagc acattgttac catagaagac cctgttgaat
    90421 ttatttttac caataaaaaa tcaataattt ctcaaagaca ggtgggagtt gatactcctt
    90481 ctttccatga aggagtaaaa tatgctctca ggcaagatcc ggatgtcatt ctgataggcg
    90541 aaataagaga tagagaaacc atagaaagtg cccttaaggc ttcagaaaca gggcatttgg
    90601 tttttgccac acttcataca aacgatgcag tacagacaat aaacagaata gttaatttat
    90661 ttgaccctca agacaggcaa tatataagaa atcaaatatc aagcaccttg cgcggaacta
    90721 ttgcccaaaa acttcttaac aaaaaaaact cggacggaag agtccccgca cttgaaattt
    90781 tagtttcaac ccctacagta aaagatttta tagaaaaaga tgaacttgag cagatatatt
    90841 cacttgttaa aaaaggttct ttcaacgaaa tgattacaat gaatatgtct ttgcttaaac
    90901 ttgtgcaaaa tgaaattata acaaaagatg aggcgattaa tgcttcagaa aacaaaattg
    90961 agcttcagca aatgctaaga ggagtttacc atggcacggc agaagacggc ggagtttaat
    91021 taatgaataa ttttgtaact gatgccatca acttaaaatc ctataactta agcgaaaatg
    91081 acaaaataat tttaatgtat tcaaaagaaa aaggattgat aaaaggcgtt gccaaaggct
    91141 gcaaaaaacc caaaagtaag cttggtgcaa gaatggattg ccttatcgca aacacattaa
    91201 tgctctacaa aggtaaaaat ctcgacacaa tatgtgaagc acaggcaatt aacactttca
    91261 aaaacagccg tcaggacttg gataaacttt ttaattcggt ttatatttca gagattgtag
    91321 gaaatttcgg aatggaaaat gacccggcgt ctgaagaaat ttacaacctt ctttacaaag
    91381 cactggaaaa aatttcaagc gcaaaagata aaaaagatat tttaataagc gtaataaaat
    91441 ttcaactgaa attcatgcag ctttcaggct ttggtcttga gcttgacact tgtctttgtt
    91501 gctctaagcc tgttttagaa gaaaacatgt atttttcgtc gcaaaaaggc gggataattt
    91561 gtgagaattg caaaaaaggt gagataaaac ttcataataa actcagggat tttttaacgg
    91621 caatgctgca atttgatttt gattatgaaa gcgaatacga caaaaaagca accgaaaaaa
    91681 tctgcaatgt gtgttttgaa cttttaaaag actacattca acttcattgt ccaaaaaaaa
    91741 ttaaaacaac aaaaatcatg cacgaaatcg cttagattaa agctttcatg aagcttttcc
    91801 gatttattaa aaaatttttt tatacaaatt atgaagacaa atgttaattt ttttgtcatt
    91861 ttagcaaaaa ctgggcacta catgttttat aataataata tcgggtgttt attcagtagt
    91921 gtgataaggt tagaaaattt tgaatcaaaa caaacaagca gaagacaaca aaaaggtctt
    91981 gtatagtggc aatggcagct ctaaccccat taaagacggg gggattcttg cgcgcataaa
    92041 cactttaaac accagaacaa atttaaataa agtctacgga ggatatagcc accatcttta
    92101 caatgtttct acaagcgttg attattcgga tgttttgcaa gcactaaatg aaatgacgca
    92161 aaataaaaaa actctcagtg acgtttttta ttctatccat agtattttag tgaacaaaat
    92221 gaactgtttt ttcactgcct acgggattta tcaaaaccaa tcaaactata ttaaccttaa
    92281 gcttattgac agaataggcg gaacatattc ttcaaagata ttttctgctg aagaaaatca
    92341 ccctatcatt acaagctacg gcaacagggc tttagaattc aagtcagatt caagtttttt
    92401 aaatattcct tatcttaaaa actcccccgt tgtaatttta cctttaatat ccgtcgataa
    92461 ctgcatagga gttttactca taggtgataa taatcctcag gcaaatatag atttttattc
    92521 tcttcttgca agccactttg cactatttgc acacaatact gacttaatag aaaaagtgac
    92581 agaaaccaca aacacggatt ctcttactct tttgaaaaat cacagcggat ttcaagaaag
    92641 ctttgcctgt gaacttaaaa aaacacaaga ggcaaacacc aatttgtcgg taatattaat
    92701 ggatataaat aatatttcaa aaataaaccg cgaattggga catgcaaaag gcgatgaaat
    92761 aataaaactt gtcgcagaaa aagttaaaca aaacatccga aatacagata tagcaggaag
    92821 atacggggga gatgaaatag ctgtaatttt acctgatact gatacatccg aagcaaaata
    92881 catagcagaa tttttaactt acagtttatc ttgctgtttt gttgatgatg tcggacctgt
    92941 caaggtttct gtcgggattt caacatatcc gagctgctca agagagcaag aaaaactttt
    93001 aattcttgcc gaacaggcca tgtttatatc tcaatcaaaa ggttacaaag acggaatgtc
    93061 tgcaatcgta agctcacagg attataatct ttgggatgat gctgcgctaa gttcttttgc
    93121 agaagttttc accaaaaagc acgctcaaat aggaataaat tttgaagatg aacttataaa
    93181 caaattcaac agcgaaaaca ttatctcaaa aaatcacctg atagaaatgg taacctctct
    93241 tgcaagtgcg attgacgcca aagacactta cacaaaaggt cattctactt cggtttctcg
    93301 ctacgctgaa gcccttgcca gagccataga tttgcccgaa aaagaagttg aaagaattgc
    93361 cctgggagca cttttgcatg atataggcaa aataggaatc cccgaagatg ttctcagaaa
    93421 acccgacaga ctttctgatg aagaatggaa tatcatgaaa caacaccctg taatcggtgc
    93481 ggaaaaagtt cttatgccaa acgaatctct gagagatttg attccaatgg ttaaatatca
    93541 ccacgaacac tgggacggca cagggtatcc agaaaaatta aaaggtgaag aaatacctgt
    93601 tgccgcaaga attgtatcaa tcgccgacgc ttaccacgcg cttataagca accgccctta
    93661 cagaaaaggt ttaagcgtta caaaagcatg cgaaatttta aatatgggag caggtgtcca
    93721 gtgggacaga aatcttataa gacacttcat tcaaattgcc ccctctcttt ctacaaaagt
    93781 ataaatttta catttaaaaa tccttgcaaa tgcttgaatt tcattctttt gcattagcct
    93841 aaagggttgt atttgtcaca aacaatgcta taatataata atagtgttat taagaagttg
    93901 aaagaggaac tttatgtcta tatcaatatc aggactaggc tcaggtttac ccgtagatac
    93961 ctggatagaa aaattattag ccctaaaaca agcaaaaata gatgaaattt cacaagaaaa
    94021 aacttttgcc ggatattcaa gttcagcttt gagtactata aaaacatctt tcaatactct
    94081 tttatcttca atgcaaaaag tcaccgattc aagattcggc tctacaagcg atctttttgc
    94141 gcaaaaaacc gcaacatctt ccgattcaaa tatattgacg gcatctgtta cggctcttgc
    94201 ctcaaaacaa accgtgagtg tttctgtatc acaattagca agttcgactg tcgcaaaaag
    94261 cgtaaatacg gcttctgcta aaatgaccgg aacaaccact gtgagctctc ttgcaaacgg
    94321 cggggtaacc gaaggtaatt tcagtgttta tgtaaacaac accaaataca gcatttccgt
    94381 tgcgtcaaca gacaccttaa ataacatttt aggaagaata aattctcaaa cagggatgag
    94441 tgcaactgtt accgacggca aaataacaat ttcggaccct gcccaaacag caaatattgt
    94501 agtgggctca aattctgaca ccacaaattt tgcaaatatt gttgctctaa agaaaaacgc
    94561 ccagggtgct tatgtaagca accaatctgt tttgctggct aacacatcgg caaaccttac
    94621 ttctgcaaat gcaggatttg cacagcaaat caacacaagt tcttttaaaa taggaaatgc
    94681 tacatttaat gttgattcca gtacaacttt gaatggcttg atttacgaaa ttaataacag
    94741 tgaagatgcc ggtgtaaatg cttattggga tacaggtgca ggtaaattgg ttttaacctc
    94801 aaaaacaacc ggggcttcaa acatcaacgt tcaaaatctt tcaggcaatt tcaccgatgt
    94861 aatggggctt acagcctcaa cttttaatcc cgacggaagt attgcaacaa caaaacttgc
    94921 agataatacc caaactctgg ggaatcttgc aacgcttaca ataaacggaa cctctatgat
    94981 ttcatcttca aacacaatta caagcgatat ttcaggaatt accggtttaa cccttaattt
    95041 aaaatcgata aatactgaag aaacccctac aacaacaata acaatagaaa caaacaattc
    95101 aagtcttaat tctgcaataa gcgattttat atcaaacttc aatgaggtaa ttgcaaatgt
    95161 tgatgaagcg accggaagcg acggttacct caacggagaa agcgttttag taagtattcg
    95221 aaacaacttg cgaatgacgg caacagcatc tgtcgatatt gacggaaact acaaaaccct
    95281 tgcaagcata ggaatcacca caggtgcggt gggatcagat attaatgcca acaccaacca
    95341 gcttcaaatt gattcaacaa aacttgcagc tgcgttggct gatgaccctg atgctgtcaa
    95401 aaaactgctt ttaggtgatg atacacacga cggggttttc aaaaaactcg aatccataat
    95461 tgacagttct atggattcat cttacggata tttcacgaca agaagtgcga ccttaagtga
    95521 aaatatctca gatttaacag acactattac aaaaaagaca gaagaactcg gagactacaa
    95581 aattgaattg caagataaat tcaaccaaat ggatcaactg attgcaacac ttcaaaacca
    95641 atacagcaaa gtcatttcgg ctttaagcac aattacttca aacagttctt catagtaatt
    95701 aataaagcaa ttttaaacaa gtttctattt ctttatttgt aatgtcgttg aaaacttcga
    95761 caaacgattt ttgcacgggc aaaataaagt ttatatttcc gttttcgact tttttgtctg
    95821 attccattat ttgaattaac ttttgagggt taaattttgg aattttttta attattttgt
    95881 aattatctat aagctcaaat gcttttactt ttaagctttt tcttataatt tttctctcat
    95941 acgcaacaat tacggcaaac ttcattccta tagaaatcgc ctctccgtga ttaaattttt
    96001 tgtaattggt aattttttct atcgcatgcc ctaaagtatg accaaaatta agaacttttc
    96061 ttagcccttg ttctttttcg tcttttttaa ccacggcgat tttaagagtc agagaaattt
    96121 taataatttc gcccaaagta tttgtatttc ttgaaaaaat tctctggggg tttgcaatca
    96181 aaaagtcaaa taaatcaaaa tcctgctcaa caccgcagtt tttttctata aaagcgtatt
    96241 tgataacttc tgccataccg catttaagct gcctttcatc aagtgttttg agaaaattaa
    96301 gattacaaaa cacggctttt ggctgataaa atgcccctac catattttta cctaaattgc
    96361 tgttgattgc gactttgcca cccacggaag aatccacaca ggcaagaaga gttgtgggaa
    96421 tttgtataaa atctattccc cgaaggtaag ttgctgcggc aaagcctgcg atatcaccca
    96481 cgacgcctcc tcctatagca actatcgcgt catttctttc aagtttattt ttaattgcaa
    96541 agcggacaat tttttgaaaa tttttaaaat ttttctgccc ttcgccgtct ttgaggacaa
    96601 atttaacact gtttggaata ttaagcagat ttccgtaaag cttattgact ttttggctta
    96661 ttacgacaag aattttattg gcattggtgt gtttgaaaat cttttctcca agactttcaa
    96721 tagactcatc atcaataaat atcgggtatt ttttacctga aggcagatca acccaaagct
    96781 cgttacttaa catgtccctc atactctttc agaatactgt caagaatagt ataagcctgt
    96841 tttttattcg tgtcaatcac aaaatccgct tttagatagt ttctgcgcct ctttgagaga
    96901 agttttttta tttcttcgag cgaattttca acgttaagaa gcggcctttt agtttcgttt
    96961 ttaattctac tgaaaagttc atctgatgtt gcgctaagat aaaaaataat cccgtttttc
    97021 tttaaattaa gtacattttt ggggctttga caagttcccc cgcctgttgc gattatttga
    97081 ttttttaatt ttgaaaattt ttcaataatt tcctgctcta gttttctgaa ataatcttga
    97141 ccgaattttt caaaaatttc acaaatattc atttttgctt gtttttcaat ttcgctatcg
    97201 atatctacaa agtcaaaatg aacaagaatc tttgcaagct tatttcctat ataggtttta
    97261 cccgctccgg gcatacctgt taaaactata tttttgttgt tttcttcagc cgacacttta
    97321 tcccctatag ttttttgtta tttcagtaag tgagtctttg ccgaatttct ccaaaaattc
    97381 atcggcaaga atgcacgctg acatagcttc tgccacaaca cagcaggctt ctaccgcaca
    97441 ggtgtccgaa cgttcaaaat aggattcgac ttgagaatta tctttaaaat caacggtact
    97501 taaagctttt ttcattgtcg gaataggctt cattgaagct tttatcacaa tgttttcacc
    97561 gtttgaaatt ccgccttcta tcccgcctgc attatttgtt tttctcaaat attttccgtt
    97621 ttcaaaaaat atttcatctt gcatttgact gcccaaagtt tgcgaagatt tttcacccaa
    97681 accgatttcg accgatttta tcgcaggaat actcataaca gcctgcgcca aaagcccgtc
    97741 taatttcctg tcccagtgta catgtgagcc aagacctaca ggcaaatttt caaaaataac
    97801 ttcaaactta ccgcccagag tttcgccctg cccttttgcc caatcgactt tttctttaat
    97861 tttttcttct tcgctttcgc ctgctatatg aagaattttt gaaaaacaat ttatcccgaa
    97921 ttcttttaaa ataatttttg caacagcacc gaccgccaca tcaacggctg tttttctggc
    97981 acttgaacgt tccagaacat ctcttaaatc tttcaagtca tattttatcg cgcctgcaaa
    98041 atcggcatgg cccggacgtg gagcactgaa agatttttca tctgtaaaat cgtgttcttc
    98101 agtgctcata attttttgcc agttttcaaa atccttgttt ttaatctcta aacaaatcgg
    98161 cgcacctgtt gtcttgccga accttaagcc tgatttgatt tcaaccgtat cggcttcaat
    98221 attcatccgc tcgcccctgc cgtagccttg ttggcggcgg ctcagttctg aattaataaa
    98281 atcaaaatca attttaagtc ctgctggaac accttctatt attgcattta agcactgccc
    98341 atgggattcg cctgatgtca gaaatctaaa attttttaac acttttcata cctcaacaat
    98401 ttttaattat aaatgaactt actcacctat tcacctattt acttattcac ttatttagca
    98461 tacttaagaa aaatctgctc ctttgtctgc atcagttctt ctacaataag ccaccttccg
    98521 tcagactgtt tttgcacaac catcgaactt ttaagataat aattgctcaa tgacggcctt
    98581 ctcaaagaat caatcctgta accgtttgaa acttttgtaa cttttatgtt ggaataataa
    98641 tttttataat ttttaagttt agcgattttt tcgctcaacc gcatttgtgc tctgtactgg
    98701 tcagccgtaa aataagcgtt tgcaaggctt ccgtcgggtt ttacaatttg tcttatgatt
    98761 tttgcattat ccgaatacat cgaaggaaca gcttgactgt aactgtttgc ggcatttaca
    98821 aagctgttaa aaaatttcaa agcatcttcc tgcccgtctg caaaagtgca taagccaaac
    98881 atcattattg taaaaacggt cagtattttt ttcattaaca aaatccttaa gtgtttttaa
    98941 atgttaccat aaaaaaaatt aaaaatatca ccctttaagg ctaaatatta taatgataat
    99001 caggattatt tttcaatttt tcttctatct catcaaaagt caaaaagcct tcgcttgcac
    99061 cgaattttga cacaacggaa gccgaattta cagaagcgta cataagcgat tttcctatat
    99121 caaaaccggt ttttgtaagc gaagcgcaga aagtcgatga gaaagcatcg cccgcaccga
    99181 gcgttgaaac aatttgaacg ggaaaaggcg gacattgaaa gaaagttttt ccgtcatagg
    99241 cgtaaacccc gtttttgccg tctgtgatta ctattatctg ctcatctccg acttttaatt
    99301 ttttaagcat ttctttaatg tcggggtgga tgagttcttt cgagaaattt tcatttctgt
    99361 tatctggtct tacctgaatt ttagtgcaca tcatagcttc ttctttattc ataacagcca
    99421 cctgcgccgt atcaataatc tttttaatat attcaaaccc tcttttaaga cttgttgtac
    99481 ctgcattaaa gcaaacttta accccgtttt cgtttgcaaa ttcgactata gattgcaaaa
    99541 ccctgttgga ttcaccgctt aaaggcgcga tataaagcag ttttgcattt tttattgcat
    99601 caaaatttat ttccgatttt ttcaaagtgg catttgcccc tctttgtgca agaaccgttc
    99661 tgtcgccttg gaaactcatc aaaattatcg aaaaacccgt cgaaactgaa ttatcctgaa
    99721 ttatgtttga aaggtcgaga ttcggtttac aacaaagtgc ctgtaaaatt ccttttgaat
    99781 aaatatcatc gcctatctta caaattgcgc ttgtgttaaa acctaaattg gcaaaattta
    99841 gggcggtgtt aacccctcct ccgcccacat ttgaagcaaa agccgacact tccatttttg
    99901 caccgtaggg gtaactcata aattcggatt ttgaattttt tgtcgaaacc gacactatat
    99961 ttgcgtcttc gcattccaca aaaacgtcca ttgtcgcact gcctattgtt attacgtctg
   100021 tcatcttttc tcctttttta agtcattgcg agagggaata agcataaatc taaatcattt
   100081 accccttcac tgctcacctc tcacccttca cctttacgct ctcgggtgcg cctcatcgta
   100141 aacttgtttc attctttggg ttgaaacgtg ggtataaatc tgtgtgtttg aaatgcttgc
   100201 atgacccaaa agttcctgaa ctaccctcaa atccgctccg ttttcaatta atctggtcgc
   100261 aaaactgtgt ctgaaaacgt gaggcgtaac ttttttgggc aattctaaag aattaacgac
   100321 ttcgttaatc acgtttctta tggttttatt ctgcaacctg taacctgtgt tatttatgaa
   100381 aacaggaacg ctgtcgccta tttgctcaag cggaaaccct tttgccacaa gcaaacgggc
   100441 tgtgtccaaa tacctttgaa gataagtttt tgccctatcc gagaccaaca caattctttc
   100501 ttttgcgcct tttccgaaaa cttttatttc attgttatca aggtttaagt caccgaaatt
   100561 aagtccgctc aactccgata ccctcatgcc tgtcgcccag agaagctcta atattgcttt
   100621 gttcctaaaa ccagcaggag tatcaatttt aaccccgttt aatatttttt ctatttcgtc
   100681 tgtcgtcaaa aatttaggca aagattttgg acgctttggc ggactgaggt ttaatgcagg
   100741 gtttgtttcg ataagtcttt ccctgtacaa aaacttataa aatgttctta tagatgcgat
   100801 ttttcttgcg atcgtcgttt ttttgtagtc aaaccgctga ataaaatgca gatattctct
   100861 tactttggaa aaatttacgc tcaagcacga aatatcccca agccacaaaa gatagcttaa
   100921 aacatcagaa cagtaggctt ttgaagtatg ttttgaaaag tttttctcca ccataaggta
   100981 agacttaaag tcttccaaat actgctttga ttttgcatcc aaacccataa aaaatccgcc
   101041 cctttgcaat aattataaac taattacagg gggatgtaaa gagagtaaca ggtaaatagg
   101101 tgttttagtt taaaagtcga atagtcgata agttgaaaag ttaaaaacta ttcaactatt
   101161 caactcttag actgttcaac tagttaaagt tttccgccga ctgctttata aaggcttatt
   101221 tcgttgatgt agcagtctgt tttgcttgaa accaccatct tgttaagaac aagcaaagtt
   101281 tctttctgct gaattaagtc aagccccgaa ataacaccct gttcatacct tttttgcata
   101341 tagccgtaat ctttttcctg catttgatat gcttttaagt tattgttata tttatcatta
   101401 tccaatttga gcgaactaag tgcatcatta acttcttgaa tggcaacaag atttgttttt
   101461 tgataatttt gcaaaatctg ttcatatctg tttttataaa tttttaaatt ggcaattctt
   101521 gccccgcccg taaacaaagg taaaagtgcc tgaccgccca taatagccag cgcatttttc
   101581 cagtcaaagg aagctcccag tgaacttgtg ttaaaaaaca aaagccctat gatatcaatt
   101641 ttgggcaaaa attccttttt tgccacccta acatcaatcc ctgctttttc aaccattttt
   101701 tctgccgcct gataatcagg cctttgtgtg ataacttcag atgaaatttc gtttggaata
   101761 ttcaatttat aattcatttc atcgtaagaa attcttttaa attcctttgt gttatcaggg
   101821 ctgtcgccga ttaaaaccgc aaggtgattt aagacaattt ctcttgcctt ttgcaaatct
   101881 gataaatcag tttgtgccaa tacataagcc ttttcggcac gcattaaatc tgctgtggaa
   101941 gtaatcccct gaacgtttct tattttcatc aggtcataaa tttcttttct gtctgagatt
   102001 atctgctgct gaagttcaat gagtttgtcc attttaacaa gattaaaata agcagccccg
   102061 acagcagatg ccacggaaat atatgccgtt ttttcattga attggcttgc ttcataatac
   102121 ttttttatcg cctttgtttt gtccctgttt ttcaggaaaa tatccgcttc ataatttacc
   102181 ataagcggaa atccgtaaga aacttcagtg ttggaaaccc cgttcatttt gtaaagtgcc
   102241 ggcgcaaaac cgacagaagc ggtaggtaat tctcttgaaa attgtgcttt tacactctgt
   102301 cttgcttctt caacttttaa tgttgcgatt tttaaatcgt aattgttttt tatggctttt
   102361 atgatatagc cttgtaaata ttcatcgtta taaccctgcc accactcaag attgacgtct
   102421 ttgaatttat attcgctcac ctgcgattgt ataactcttt tattttttat ttctttattt
   102481 tttaccgcca aaacaggcat tgcattcaaa gccaaaattg ctatgcttag tgtaattgat
   102541 attatttttt tcatttcatt tcctctgttt tagttgataa tgtaaatatg ttataaggtg
   102601 aaaagctttt gcttgccctc ttgccttcat ttacccccta tttacttttt cctaatggta
   102661 tgctaacata atattgttgt aattgcaaca tgtaatcatt acatcatgtt ggaactctaa
   102721 caaaaaggat ttacaataat attatacggc aggtttggat gaaagaaaaa gaaacgacaa
   102781 attattttaa cagttcgatt tatttaatgg aacaggttgt tacttacgca aaaatcaaag
   102841 gcacacaatt ttttaaagag ctgaaaatgg gtgtaacaat agaccaattt gttgctttgg
   102901 atgcggttta ttcaagcaac gatatttgcc aaagggatct gtcaaaactt atattaaaag
   102961 accgttcaaa taccggcaga attttaaata ttcttgaact gaacggtttt ataaaaagag
   103021 tcattgagac aaaaggcaaa aggttagtca aaaagattta cataacccaa agcggcaaaa
   103081 aacttgtaga agaaagtatt cctaaattga gaagcgcatt tttacaagtt tttgagaata
   103141 tttcagataa agattttatg attttaaggg aaacactgga aaaaatgaaa ctcagcttaa
   103201 gcaaaacaac cagcatacaa atttaaagga aggaataaaa tgtcaaacga aaaaaatagc
   103261 aaattaatga aaaaaagatt tttaatcccc tcaataacaa tcattgtggc aattttggca
   103321 cttatcggaa tgataaattc ttcaagatat caatcaaccg aagatgctta cgttgaaaca
   103381 cacacggtcg cagttgcacc aaaagtttca ggacaggtta ttgaaatgta tgtaaatgat
   103441 aaccagcatg taaacgcagg agatttggtc gcaaagatag attctgccga ttacggagca
   103501 aagctggatg aaatttctgc aaaatatgag gctacgcttt taaagcaaaa aaatgcaaaa
   103561 gctgtatatg aagcagcgaa ttctcaaata actctggcta aaaaaaattt agacagatac
   103621 acagagcttt ataaatcagg tgcagcatca aaattagaat ttgataacgc aaaaacaaat
   103681 tacgacaacg ctcaggcatc tttaactcaa gcgcatcaaa atttactttc aaaagaaaat
   103741 aacagggtgg cagatgccga tttaaaacag cttgccgcga ccaaaaagca agcacagctt
   103801 gcatattctt atacaaatat ttatgcaccc caatcgggct ttgtttcaaa caaaacaatc
   103861 gccaaaggtg caatagtaca ggcaggacag cccttgttta cacttgttcc cgatgatatt
   103921 tggatagttg caaactttaa agaaagccag cttgaaaata tgaaagtcgg acaagaagta
   103981 gaaatcaaag tagatgcata cccgcacaaa agatttaaag gaaaaatcga cagcattcaa
   104041 aggatttcag gtgcaaaatc aagtttattt ccgcctgaaa atgcggtggg aagttttgta
   104101 aaaattgttc aaagaattcc cgttaaaatt gtttttgcag aaaaaataga cacaagcaaa
   104161 tatacaatcg tgcccggaat gtcggttgaa ccaaaagtta aagtaaaata gtgaagtaaa
   104221 tgtccgaaaa tataaataac ttgaaaacag aagaatggaa attgccggaa aacccttggc
   104281 cggcaacttt tgccgtgatg attgcggttt ttatgtttgt attggattcc acaatcgcaa
   104341 atgtggcact tcctcacatg gcaggaagtt tttcttcaag caacgaagaa gctatgtgga
   104401 ttttaaccag ttacatgatt gcaagcggaa taattttacc ttctgtggct tggttcagcg
   104461 gagttttcgg cagaaaaaac ttttttatag gctgtgtaat tatcttcacg gtgtcttcgt
   104521 ttttgtgcgg cttggcggca tcacttgatc aaatgattat tgcaagaatt ttgcaaggac
   104581 ttgggggcgg tgctattgtg cctgtggctc aagctattct tattgaaaat tttccgcttg
   104641 aaaaacgccg tatggctatg tctgttttcg gtgtaggaat tattatcgca cctattgtag
   104701 gacctgtttt aggcggctgg attacggaca attataactg gcactggatt ttttacataa
   104761 atgtaccgat tggttttctt gcagctttaa tggcaaatat gtttatagaa gacccgcctt
   104821 ttgcaagaaa aaaagaagga caaaaaatag attttttagg cttcagtttt ttaattatct
   104881 ggcttgtttg cctgcaaatt gtccttgaca aaggaaacaa cgccgattgg tttaattccc
   104941 cttgggtttg ctggaccttt gccgcatcaa tgctttcaat ggttgccttc ggggtttcgc
   105001 agtttgtaaa cagcaaatct ataatcgatt tgaaagtttt taaagacaaa aattttacat
   105061 tcggaacaat ttttatggtt ttggttacag cggtattgta ttcttctatt gcaattatgc
   105121 cgttattttt gcaaaatctt ttgagatatt cggcatattt aagcggtttg agcatgatgc
   105181 caagaggcgt cggaagtgta accggtttaa ttataacggg tattttcgca aagaaaatcg
   105241 acgacagact cttaattgca atcggacttg cccttatggg tgtatcaagt ttaatgtttg
   105301 gatttttaaa ccttcaaata tcaatgatta atattataat tccgaatttt atcttcggat
   105361 tgggaatgag ctgttgtatg attgcaataa gtacgatttc agttgtcact ttaagagccg
   105421 aacagatgac aaatgccaca ggggtgcaaa atctgctgaa aaacatcggc ggtgctgtcg
   105481 gtatgtctat tgttgcgact tttctctcac gctttgcgca ggttcatcaa aacatgatgg
   105541 tcggaaactt aaacccgctt aaccccgcct ttcaatacaa agtaagtgcc atgaccgctg
   105601 ctttttcaaa atatttcgcg cttccgcttg cccaacaaaa agcaaattat cttatgtatg
   105661 ttgaattgct taagcagtca agcctgtggg catttatgga tgcttttaga atttttgggg
   105721 taatttgttt tataataatg ccgctgcttt tgtttatgaa gcctcacaaa tcaaattcaa
   105781 cagaagacgg attttctgcg atgcactaaa tataatcagg cttaataact ttacatttag
   105841 cttaaaataa tatacaatat tttagtcaat cgggagaagg gttttatgaa aaagaaatta
   105901 attgctttaa cgtttgcgtg cacattggca gcttcgacta caggggtttt tgcgcaagaa
   105961 gattcatctg cgcctgttca ggttcaccct gcaaagacag ataatataaa agccttcccc
   106021 gaaaatcttc ctcaaaatcc gactgtcgga cagggccaaa ctctttcgac aaaaattcaa
   106081 tattcaaagt ttcgaaaagt acataaaaaa gttacaaaaa agtatgttaa ttacgatgaa
   106141 gtcgataaac ttatcgaaaa cagctactat gaagatgcag ataaaatatt aaaaaccgcc
   106201 attaattcaa attcaaaaaa tataaaagca aaagcacttt gggttgtttc tcttgcaaaa
   106261 cagtacaagc ttgaccctgc gcaaacccaa ttaaacgaac ttttgaaaaa atatcccgac
   106321 aatgccgacc tgcactatgc gcaagggatt atttattaca aaagaacaac atcttcaaat
   106381 atggagcata ttaaaaattc gcaaaaatta tttgaagatt ccctgaaaga atttaataaa
   106441 gcaattgaat tggacaaaaa agatgccagg tattataacg cggcaggggt ggtttcgctt
   106501 cagttgggaa aagacgcgga tgcgagaaag ctttttgcca aagccgtaga gcttaacccc
   106561 aaatattcaa ccgcaataga caatatggga acaatggatt ttgttgacgg gaaattggag
   106621 gatgcggaaa aaaaattcaa tcaggcactt ttgcacaaca cccaaaacac aaccgcaatg
   106681 tatcatttgg cacaagtagc aaatcaaaga caacaatact caaacgcttt gacttatcta
   106741 aacaacgctc ttgcgattaa tccaaattct tatgcgtttc aaaatttatc aggcgaaata
   106801 tatgcaaaac aaggaaacga agctgccgca ataaacgctt tcagaaaagc ggttatgata
   106861 aagcccgaat ttgcacctgc ctactttaac cttgccgaag tctatgaaaa aagaggcgac
   106921 ggagaatttg caatcgaaca gcttaaaacc gcacttgcaa tagaccctac tttctacgat
   106981 gcaaagctta aaattgcaga catttccctt gcaagcggaa aatataatca ggcaatagat
   107041 aattattcgg cacttgccca aatagacgac tataaaaacg atgcgctcaa aggacttgca
   107101 aacgcttatt ttgcaaaggc ccaaatttct tcaaataaat cggttttggg ctcgagtcaa
   107161 gagctttaca aagcatttga tgaactaaac aatgcgataa aagcaaataa cgatgatttg
   107221 gaacttcatc tggcaaaact aaagcttgca aaagtaataa atcagcccgc aatgagccag
   107281 gatgaacttc agaacataat taactcaaac gatactacac ttacggctat ggtggtaaaa
   107341 ggcgaagctt atatgacttt aaacaacagc cctgaagcca aaaaaatgtt tgacgctgct
   107401 gccgcttgcg ctaaaaccct tgaagaccga cagtatttaa cagaaatttt tatctacaac
   107461 aaacagtacg aaagtgcgca ggcaattatc gacaatattt taaagagtga cccgcaaaat
   107521 actcaagcac ttgccaattc ggattacatt aaaaaatgcg aaaaatattc ccaaaacagt
   107581 cttaaatcag cacaatattt cttgaaatca aaaaattatc ctacagcgca agaatatgca
   107641 acccgcgcaa tagggcttaa ccctaacaac tctcaaggct atgtgatttt ggcagaggcc
   107701 tgcgaacagc aaaaggatta caattctgca atcgcaaact acaaaattta tctgaatatg
   107761 accccgactt ctgcggataa aaataaaatc caaaacaaaa ttaaatacct tgaaaacaga
   107821 tttaagtaaa accttcttaa caaaattctt gtaatttatg cacttttgta atatgctcag
   107881 agcattaaca ctatcctctg tatatttgtg ccgtcattaa gcacaaaaat caattaagaa
   107941 aggtaaaacc aaaaatggca aatattaaat cagcaaaaaa gagaatctta accgccgaaa
   108001 gaaacgctga aaaaaataca gcattcaaat cttcgattaa aacagctgtt aaaaaagtgc
   108061 ttgaacttgc tcaaggcaaa gacaaaaaag cacttgatga agcagtttca aaagtttata
   108121 aactttgcga caaagcggtt gtaaaaggaa tcttgcacaa aaatacagcc gcaagaaaaa
   108181 aatcaagact gtctcttgca ctcaacaagc tcagtgcaaa aaagtaatta atattttaaa
   108241 taaaaaacag tggtttcaaa aaaccactgt ttttttaaca aaagataaag gataatttaa
   108301 tgttaacagt atctaaaata aattctgttc ctatatcctt caaaaaatta attctgccgc
   108361 ctaaaaatta caaggtaata gacgaatatg tttcacgctc tgcccagccg gacaaagaaa
   108421 gttttttatg gttgaaaaat caaggcatta cagatgttat taatttcagg accatgtacg
   108481 agccaaagat agattttgac gagtcccaat atttaaaaca aataaactta aactatcatc
   108541 atttacctac aggcacaaga accataagtg aacttacggt gaaaaaattt attgaactaa
   108601 ttgaagaaat aaggaatccg aacagcaaaa gaaaagttca tttgcactgc aaagccggcg
   108661 cagacagaac aggatttttt acaatgatat acaaaggact ttacaacata gattcttttg
   108721 aaaataatgc aaaagaaatg cttgaaatgg ggcacaacca gggacttttc ccaaacttga
   108781 taaattctgc aaaaaaaatg attgaatttt tcaaaaaaat taaagcttaa taattcacaa
   108841 tcataaatga ttttaagagt cttcctgcag aatcgtttgt cgaaacgact tcgattgatt
   108901 ttttgtaatt cattgaggtg gaacttccac cgtcaaatgc catagctttt tcaaacccta
   108961 gctgctgaca caaatctctt gcctcataaa ttgtcatcgg attatcgttt gtgataatta
   109021 agatatgaat atcctgacct ttaagcccta taattgttct tgcggtttta tcaagaactg
   109081 aagcggaagt gcggacaatt ttcccgtctt ttttaagtat aaaaaattct tcttccagtc
   109141 ttaactcggg caaaagcata ggaccgccct gtgctgaagt tacaaccgaa caagcaaaat
   109201 caagatttgt tttatgaggg actatttcat aattaatttt tccgtcacaa tccaccactc
   109261 tgaattctgt tctgttaata attttgtcca aattttgcct taaaacaggg ttcatcaaca
   109321 aattttcgtt aaataaagga tctgcagaaa tatttctgtc ggttacgata taggaaatgg
   109381 ttttttgatt tttaggatca aaaaatcctg tattaatggt gaactttgag cgtgaagttt
   109441 catgcgcttc tttatttgtc attaattttt cacttgagac aaatttaatt cctttttgaa
   109501 ctcttttccc gtcaagcgta atgtgataaa ttccgttttg ataatcaatt tgaacaggct
   109561 cggaaacaca aaacccgcaa gaaatcattg ttatacaaaa taaagcaact attgttaaaa
   109621 aaaatctatt catacaaatc ccttaaaaat tgttggtgga ataacagtcc cgccctaccc
   109681 ctgttcacag ttcacggctc acccttcacc ctataaatcc ttcgatttat aaaacgccca
   109741 aattgcgcaa gtaaacgcaa taagcgggaa tgaagcagtt atgaaaggat gtaaaacgcc
   109801 tttttctgca agcaggtcaa agaacggaag cgttatgtaa taaaggaaaa tacatccgat
   109861 agcaatggta aatcccacca aacgctgctc tctgggtttg ctaaatccga gcagacatcc
   109921 taaaatggca agaagaacac acacaaacgg gtggaaaaac ctttggaaat atttattgag
   109981 cataaatctg tattcttcct gcaaatcttc ttgtttaagc agttttatat atcttcttaa
   110041 atctccgttt ccgatttctc tgtcgcgttt aagcgaataa atcattaaat cgtaagcatt
   110101 ttgtgccgct tttccttgta aaagttccaa attatttaag tgctttattt cttcaaaaat
   110161 tccgtcattt gaaatacgat attctgtaac atcttttaaa atccactttt cagtataatg
   110221 gtcggcattt tttgccacaa aaatatgaga tagcgcgtgc aaatcggtgt aagttttttc
   110281 cgaaaaatca agcacaataa catttgcaat attgctgtaa ttaaaccttg aaactataac
   110341 agccaaagtc gggcggtttt tttcatcctt ttgagtgtaa atatactgtg aagggctgta
   110401 ttcgcgctta attccgacaa gtttacttgc cgaaaacgga attaatttgt cgtaagtaac
   110461 aaaacaaaaa gcagtaataa gaaggcttaa aactataacc ggcgaaagaa tccgccaaaa
   110521 agaaagccct gcgaccctaa agattgtaag ttcaaaatct ttgctgagtt tatcaaaagt
   110581 aaacaatgaa ccgagcaaaa gccccaccgg caaagctttg cctaaaatat tgggcagttc
   110641 gtagaacaac accaaaattg ccgtttttgt cgtatattct ccttcaagag tttttcttat
   110701 tgtatttaaa agcatctcag gagcaatcca aataacagta aaaagcaaaa ttgataccat
   110761 tgtcgcccaa aaaacctgct caaaaacata tttgtcatat aactttattt ttgaaaacaa
   110821 tttcattaaa gctcaaaatc ctttcctaaa taaaattctt ttgcgatagg gtcattagca
   110881 acatcttcgc ttttgccttc aattttaatt ttgccatcaa aaattacata ggccctgtct
   110941 gttatagaaa gcgtagcttt ggggttatgg tctgtaatta aaacgccaag ccctttttca
   111001 tcagacaatt tacggatgtt ttcttttatt tcacctattg caatagggtc aattccggta
   111061 aaaggctcgt caagaagaat aaaatctgga cttgcggcaa gagctcttgc aagctcaacc
   111121 cttcttcttt ctccacctga taatgatacc gccgattctc cgcgaagttt tattatcccg
   111181 aattctgtca acaattcttc caatttacgt tttttctcgt caacggtaag tttttcgttc
   111241 atctctaaaa caagtttaat attatcttca acacttaact ttctaaaaat ggaagcctcc
   111301 tgcggcaaat atccgacacc tgcctttgca cgaacattca tcggccaggc agaaatatcc
   111361 tgatcattaa tataaacatg ccctttgttg ggtttcacaa gccccactac catgtaaaaa
   111421 gtggtcgttt tgcccgcccc gttaggaccc aaaagacaaa ctacttctcc tttgttaatt
   111481 tcaaaagaaa cgtcatttac aacgcttctg tccccgtata ttttcacaag attttttgcc
   111541 gtaattttca tattttcctc acccttctta tgataatttt atgttaaaaa caatctcaat
   111601 gcaagaaata gtaaatagga aatagaaatg ttaaaacagt ggacaagagg cgaagatgcc
   111661 aagaagcgta gtaggataac ccacgcatct actcatctta tcttcttata aactattcga
//
LOCUS       DAOQAU010000002        69478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_256_length_69478_cov_8.423332, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000002 DAOQAU010000000
VERSION     DAOQAU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 69478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69478
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_256_length_69478_cov_8.423332"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3079)
                     /locus_tag="PKI94_00570"
     CDS             complement(<1..3079)
                     /locus_tag="PKI94_00570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta''"
                     /protein_id="HNW25266.1"
                     /translation="METKYKNAKKSLDYINKIMDKKSLNNLLSQIYLEFGGAKTATLA
                     NSLKNLGFRYATKAGTTISIADLQVPETKKDLLKDAEKEIEKSTHRYLKGEITEVERY
                     TKVIDTWSETTSRLTESVVENFDRLNPVYMMAFSGARGNLSQVSQLVGMRGLMADAQG
                     QIIDLPIKSNFKEGLSVTEYIISSYGARKGLVDTALKTADSGYLTRRLVDVAQDVIIR
                     DVDCHTDKFIIMTSISNGEKIVAPLADRLLGRTIAQDILDEKGKVLIPCGTTLNREDV
                     KKVAALDLQEVKVRSGLTCGLEYGICQKCYGWAMTTQKMVDVGEAIGIIAAQSIGEPG
                     TQLTMRTFHTGGVFKGAGANREIMAKEAGEVISKVKTRELRTRHGDVVQVATYEADIE
                     IKTAKGATKYHIPAGSTVFFANGMVVKKGDKLAEFEPGSQGDGSRLTEKATKDITSDL
                     SGEVLFEDFVADEKKDRQGNVSRTANKNGIIWVLGGDVYNLPGGSKVLVKDEQKISKG
                     EKLAETLTISEHGGEVRVGQELVIEEVQFEGKKIKKIVQGKELTIVIASLIPSNATFE
                     QTKKEQIWTVKDSGEKYIVKSPVDTTVENGMIIAELIDDECSVKSSGEIRYVDVEVDD
                     NQIITKPGDIVFIPEEIHQINKDANLKMVESGTFVTAGTEIIKDIYCHLDGFVELKEY
                     NDVIHEITVRPGEIHTLSSVSELKVEEGEIVKKGTLIAEGIKAKATSIVTILESDIDD
                     LDIDDLDEELDSSLHLDEENIANQPVQILIRPVQVMSVKQKDVSIKFNTTEELIDIVP
                     VTQLQYKDGARVRGLDGATLTRTSLVLQMQGYLSHLKGMVELDSKNNLKVVVLENLIV
                     RREVEGSSKLSSSMQTELLVKHGQTIAAKTPVAKTQVLAINDGVASIKKAKEDARRLL
                     LICDNYEVKIPIKTKPCVKEGDFIKLDSVLSESGEKSNVSGKVVKVSKTEIVVRTGRP
                     YLISPGTMLQVGSGALVLRGDMLATLVFERQKTGDIVQGLPRVEELLEGRKPKE"
     gene            complement(3195..4874)
                     /locus_tag="PKI94_00575"
                     /pseudo
     CDS             complement(3195..4874)
                     /locus_tag="PKI94_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            complement(4961..8278)
                     /gene="rpoB"
                     /locus_tag="PKI94_00580"
     CDS             complement(4961..8278)
                     /gene="rpoB"
                     /locus_tag="PKI94_00580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNW25267.1"
                     /translation="MSKTQYAKRVTPMGIPNTRLDDLPDLIEVQKKSFEWFLKEGLKE
                     ELLSFSPIKDYTGRLELHFLPNYTFDNAKYTIEEARIHDTTYAKQLRVMVRLVNRDSG
                     EIKEQEVYIGDIPTMTDKGTFIVNGAERVIVSQIVRSPGVYFKREISPTGKRLYNATL
                     IPNRGAWLKIETDSNDLVYVKIDKNRKILATTLLKAMGISAPEMETLFTHAEFLKKTL
                     EKDTAETSDDALVEVYKKLRPGDPASPQGGRSILEARFFDEKKYDIGRVGRYKLNKKL
                     NLNVHESQRTLTVQDIVASIEYLINLTYDEGTIDDIDHLGNRRIRSVGELLQNQFRVG
                     LSRIERIVKERMTLQDSETLTPLNLLNTKPLVASLKEFFGSSQLSQFMDQTNPLAELT
                     HKRRVSALGPGGLMRERAGFAVRDIHPSHYGRICPVETPEGPNAGLIGSLATHAKVND
                     YGFVESPFFVVKDGVVTDEVNYLSADEEENYRIAPSDLQLNKDKSIKHEYVPVRYRSE
                     FTMGESKRVDYMGVSPIQIISVATSLIPFIEHDDANRALMGSNMQRQAVPLLITQRPV
                     VGTGLEARAAKDSGMVAVATVDGVIERVVGEEITIKDKAGKLHVHQLMKYLRSNQDTC
                     INQRPIVREGDKVNAGDVIADGASTCGGELSLGKNILVAYMPWEGFNFEDSILLSERC
                     VQDDIFTSVHIEKLEIDARQTKLGPEEITREIPNVSEESLRHLDERGIVRIGARVYAD
                     DILVGKVTPKGESEHPPEEKLLRAIFAEKARDVKDNSLRVPHGEGGRVVDVKVFDREK
                     GDELPPGANTVIRVYIAQKRKVSVGDKLSGRHGNKGIVSKILPQNDMPFLPDGTPIDI
                     VLNPLGVPSRMNVGQTYECLLGLAAYLTGNHYEAPPFDEMYGDNQSEIATKEELIKGI
                     KSSGCDWVREDGKVTLYDGRTGEAFEQPIAVGLAYVLKLVHLVDDKIHARSTGPYSLV
                     TQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTIKSDDVNGRNRAYEAIVKGD
                     NIPRPGIPESFKVLVRELQSIGLDITVAKKDGVEVDLMSDMDDLRKSAPKRTLSDIDS
                     ELLNINFFETPATFGEL"
     gene            complement(8669..9673)
                     /locus_tag="PKI94_00585"
     CDS             complement(8669..9673)
                     /locus_tag="PKI94_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNW25268.1"
                     /translation="MGLTTYLGIDVGSVTTKVALVDEKGKFIDSYMLRTSGKPVLAVQ
                     EGLRELYKKALCEYDVKGVGTTGSGRNLAGALVGADVIKNEITAHAVAASINVPGVQT
                     ILEIGGQDSKIIILRDGIVTDFAMNTVCAAGTGSFLDQQANRLNVPIELFGETALKST
                     NPARIAGRCGVFAESDLIHKQQLGYPVEDLLYGLCQALVRNYLSNLALGKELLPTVTF
                     QGGVATNSGMVKAFEEALNTKVVVPNNHQTMGAIGAALLAMENHQYTGNETKFKGWQV
                     GEMHFNSITCDCHDCANNCEVITIVEGECEVLHVEKIKDIQGNIIARWGGTCGKWAIG
                     "
     gene            complement(9786..11042)
                     /locus_tag="PKI94_00590"
     CDS             complement(9786..11042)
                     /locus_tag="PKI94_00590"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNW25269.1"
                     /translation="MFDMFFIYGILSTIAFVLILPFWLVLGVFKPKLLVGFKQKCGFY
                     SSPFTLHSSPIVFYGISVGEVLAMENLIRKTKEKFPQKNIVLMTGTQTGQEIAQKKLS
                     EVCSFISYFPYDFPFCINNFLNKINPEAVFIMETELWPNFAYILNKKKIPLFIINARI
                     SDGTYKSYKKLSWFFAPILHKYTGIYPQSQKDMEKLISIGANPDKTKVMGNLKFDIKK
                     PDTKDLNFNKNPNNKILLAGSTHSGEDEIILDVFGDLKKEISGLKLIIAPRHPERIES
                     VLNLMQKKGKTARRSLEWKFSDEDMLLIDTMGELSKFYSICDVAFLGGSFNKTGGHNP
                     LEAIIFEKPVISGSNIRNFKDIYTILTSTSAGKVVQNKQELKNELKKLFTDEDYYKKA
                     SQDCKKVFEQNQGALDFVINVIQTLV"
     gene            11288..12436
                     /locus_tag="PKI94_00595"
     CDS             11288..12436
                     /locus_tag="PKI94_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNW25270.1"
                     /translation="MQDKTFLMIPGPTPVPESVLLAMAKHPIGHRSAEFSEILKSVYK
                     DLKWLFQTKNDVFIYAASGTGAMEAALSNLVNQGDKILSLIIGNFGARWAKIAQSRGA
                     VVEKLEVELGQVINPQDLRTKLAQDTMKEIKIVTLTHSETSTGAKNDIKVLCEIIRKH
                     GAISVVDGVTSVGAMNVKMDEWGIDVLVSGSQKGFMIPPGLAFLAANERAWQVHEKCL
                     YPSFYFDWKAHKNALTDDTTPWTPPINLIFALHEAFKIMKNEGLENIYTRHQNLAMGL
                     RYAVRALGLELFVKEDKNASYAITSIYPPKGISVPDIRKVFKNDYDIVVANGQNQLKD
                     KIFRMGTLGFVSERDLLTAVSSLEAVLQKLGHKFEKGAGLKAVLEYLK"
     gene            complement(12428..13180)
                     /locus_tag="PKI94_00600"
     CDS             complement(12428..13180)
                     /locus_tag="PKI94_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25271.1"
                     /translation="MQVSAIKSFQKNNILYSNKTNSLKNNHQGSVSFTQQKYIESRDF
                     LKQDWVESQDLYTRYKDSSINLEKELKRLDYYIQDAENQRDMADQNFYNEMNPKKNYE
                     INTNKFIEYWDNPYYPKTIEIRTPSGEQKYEYYMTGKIKKIQIEDFKTQEKAVFYFEH
                     NNKAPFYEEVSYYDKISKENVKIIKTKIKPSQSLIYVYTAEVQGCREKEDKHLSWFEN
                     TSNKKEICILNENNHSAFIYDQFGLPQYKQLF"
     gene            complement(13236..14981)
                     /locus_tag="PKI94_00605"
     CDS             complement(13236..14981)
                     /locus_tag="PKI94_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25272.1"
                     /translation="MKKTFEIALDEKELKKRTSKTYLVPKKMLKKDSKEYKKLKIGDK
                     KALKHLIKAAIILDEVYLKQDNPNNIEFREFLKEEIKKGNKKAKMAFKLFQAQRGIIA
                     VDTESNKIILAKGEKESAGKGFYPNDLGVNEFHEILIQMLKDCKIKEVKEILNQRSMI
                     VRDKKELKALDYTNAFEKEFKEAAQELEEAAKYSTNKVFNEYLILQAKALKENNPMLD
                     AQADKKWASLQNTPLEFTISREQYSDEMTGSVVENKELKALLKKHKIEPLSKDSIGTR
                     VGIVNKKGTKTLLEIEKYIPLLAKNMPFYKEYNQKIKQTKKQECIYPQTMVDVDIVTL
                     SGDERAFRGGMTLAQNLPNNDKLSLTIGGGRRNVYHRQVRQISDYKKLQKRLDAILDK
                     KLHKFYDKEADHWFTIGHENAHSLGPKEGTEALGKYKNIIEENKADMASLAMLDILTR
                     EGMYSEFQKKQILVSFMADNFLKAKPTLSQAHRVRSVMQTKYFLENKAIKVDDKGILH
                     IDLEKMIPTAQKMLKDIIRIQLDGDFKKGENYVLKHFVWTDEMERVAKKLKKIDKTLN
                     GIVLTPLADRIVKNI"
     gene            15079..15306
                     /locus_tag="PKI94_00610"
     CDS             15079..15306
                     /locus_tag="PKI94_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNW25273.1"
                     /translation="MLLLYVLEGCPYCKKVITHLEEKNIEHRRLDVNDPVNMDELLHL
                     GREDQVPFLVDTEHNVKMYESDEIIQYIDTL"
     gene            complement(15301..16389)
                     /gene="recF"
                     /locus_tag="PKI94_00615"
     CDS             complement(15301..16389)
                     /gene="recF"
                     /locus_tag="PKI94_00615"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNW25274.1"
                     /translation="MKILETELKNYRNLEKKQINLDFSKILFVGKNAQGKTNILESIY
                     FLSTLKSPRTSNIQEIPTFGEDNFEIKTKIKKTGVETGINYLYKEKKRELKINGLKTT
                     PKNFKNILSAVLFTTQDLLLLRGSPQDRRDWLDEVISKIYPAYDERLSKYNKIRIQKN
                     NFLKDYNGNQTLLDVYNEQLCILGSNIIFLRKKFLKDFEKLSQEKYKKISKDEKLTIF
                     YTCPFLAEEEQIEEISQKFMQKLQERKTEEIQRGQALVGPHRDDVSFFINNKEAIKFA
                     SQGQQRTIVLSLKLAEIEIIEQKTGEKPVLLLDDVLAELDDLRQNYLLKTIDKNTQTI
                     ITSVDTLLFDKKFLKDVKIYKVEEGKIL"
     gene            16650..17768
                     /gene="dnaN"
                     /locus_tag="PKI94_00620"
     CDS             16650..17768
                     /gene="dnaN"
                     /locus_tag="PKI94_00620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNW25275.1"
                     /translation="MEFVVKKEDLFNGIKIVERATAMKGLQPVLANILIETINNKTLK
                     LSATDLDLTVITTIQAQIEKEGKITLPSKTLSEIVTRLSEGLISFSKTTDTNTVEIKS
                     KNSKFEIIGISANEFPSIPEIEIKEENSVEIEIKPFTKAIKQAGFSAAGYETNNLLSG
                     VVCDISQNTLEMASTDGNRLCRVREKIENSGKTSNLIIPSKTLQEFIKMSSFIEEDKI
                     KIYTDKTKLIIKSEDITIISRIMEGQYPKYNQLIPQESPKEAIIKIGQLIAAIERVAI
                     MVNEKTSIIKMEFSSNKLVLKADTPDAGASEEEIDIDYKFEELNIAFNYRYLLECLKN
                     METEEVKIGLNSSLSATVLKPNNEDDYICLIMPVQIRG"
     gene            complement(17765..19072)
                     /gene="trmFO"
                     /locus_tag="PKI94_00625"
     CDS             complement(17765..19072)
                     /gene="trmFO"
                     /locus_tag="PKI94_00625"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNW25276.1"
                     /translation="MKANVIGAGLAGSECALQLAKRGIKVNLYEMRPEKTTGAHKSCD
                     FAEFVCSNSLGSFDVTNASGLLKKEMEILGGELIKVAYLCQVPAGGALAVDREMFSKK
                     VTEAIENNPNIKTIRKEIISIPNTPTVVATGPLTSNLFAQNIKEFTKSENFFFFDAIA
                     PIIEKDSINFEKAFWANRYDKGEATYINCPMNKKEYETFYNVLISSEKNELKDFEKDA
                     KFFESCLPIEVLASRGVDTLRFGPMKPVGLVDKKTGIENWAVVQLRQDNMAKTLYNMV
                     GFQTNLKWKEQKELIKLIPGLENANIIRFGVMHKNTFINSPKILFPSLQTKSRKDLFF
                     AGQITGTEGYTESMATGLLAGINMANYLKKEPLLILPQETMLGSLCKYISFESHKSFQ
                     PVNSNWGIVAPIEIDKKERKNKKLKNELLAKRSLNYLSNNNFL"
     gene            complement(19103..19183)
                     /locus_tag="PKI94_00630"
     tRNA            complement(19103..19183)
                     /locus_tag="PKI94_00630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19148..19150),aa:Leu,seq:gag)
     gene            19479..19661
                     /locus_tag="PKI94_00635"
     CDS             19479..19661
                     /locus_tag="PKI94_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25277.1"
                     /translation="MRNYDYESFKNALGKMKEDMYLELQDDIEAVKKNVENLLEKVFE
                     SSTFKAETRHPGVSGF"
     gene            19707..20000
                     /locus_tag="PKI94_00640"
     CDS             19707..20000
                     /locus_tag="PKI94_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25278.1"
                     /translation="MVDEVFFHALQKNINKKIKKQTKAEKENLKNELSVFQEKKVVFN
                     NDFPFLQRIEVTNKNGKETYKHYELKDGLKPEELIKDPNWRNNMDYWQEVDVN"
     gene            20035..20991
                     /locus_tag="PKI94_00645"
     CDS             20035..20991
                     /locus_tag="PKI94_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25279.1"
                     /translation="MVENVDNNALFGRLVVKSGDGIAQTILKAAKANPSIFEISEGTV
                     NAEEKRRVKEVLKEIQAERRRDGRPDICRRNNIDLVYKGDVLEFTPEEQKRIMEAMGL
                     GLKKQDKISLEDAREFFGLSGQEGEGGFQKSKQTELNIPFENTHKPYQQSYVEALPPE
                     EGQTLPPPNDTINTENNNVENPFTPANQAPEEDLDIEIKIDEPQINDTLPVSPIEIEK
                     VEQKIVNIDTPLQKTSGINTTNPNVSEVNKTPRSPEFDEKIGQITGKVQELTGLYSRI
                     AKLTPDIKNLASELDQMDASPEQKEELKILLSGVSEALGETK"
     gene            21095..21673
                     /gene="def"
                     /locus_tag="PKI94_00650"
     CDS             21095..21673
                     /gene="def"
                     /locus_tag="PKI94_00650"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNW25280.1"
                     /translation="MSVKKVLRYGEKSLREPSKEVHKVSRKIQDLVQDLLDTLYSQNG
                     VGLAAPQIGENHRVFVIDTSSGDEPLNPMVFINPKIIKKSGAFVSHEGCLSFPDMYTN
                     VRRYKNVMIKAIDEKGRSFVLEAKDGSLLAKAIQHEYDHLDGILFIDHAINRFEAEQE
                     LTQKHLPPLEVEKLLEEPELDEELKKEAKQAK"
     gene            21673..22620
                     /gene="fmt"
                     /locus_tag="PKI94_00655"
     CDS             21673..22620
                     /gene="fmt"
                     /locus_tag="PKI94_00655"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNW25281.1"
                     /translation="MIKVIFFATPKIALKSLDYLVKSDKIEVLAVVTQPDKPSGRGHK
                     ILPSSIKEYALEHKIPVFQPHSIKKEIQIQEALKKLSPDFFVTFAFGQILSKEVLQIP
                     KYVTINLHASLLPLYRGANPIQWALINGDKKTGICTMITEEGLDCGAICLKDEINITP
                     NMNYVELFELISQKSPELIETTLLNIKSGALKPTAQCCENVTYAHKIKKEDSKIDWSK
                     SALQIHNFVRGLYQFPSAYTTFKGKNIKIMETRVNEGVNNTNKTQNGEIFDVCKDGIK
                     VVTTDGVLIIKRLKPEGKSEMSSKDWANGIKNLIGEKFE"
     gene            22617..22979
                     /gene="aroH"
                     /locus_tag="PKI94_00660"
     CDS             22617..22979
                     /gene="aroH"
                     /locus_tag="PKI94_00660"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNW25282.1"
                     /translation="MITKGIRGAITVEENTKEAIEAAVVELIEQIFFKNNIENDLISH
                     IIFTTTKDLTAEFPAKIAREHFDLKNTAMICAQEINVENSLKMCLRFLIVVNCLENFC
                     PKHVYLKGAKNLRPDIKM"
     gene            23029..25035
                     /locus_tag="PKI94_00665"
     CDS             23029..25035
                     /locus_tag="PKI94_00665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNW25283.1"
                     /translation="MKRFLTILGLVLIVIFIQVLFKPFFNKLEWMTYDLRARFSTDKG
                     PFNKKFNKADKKIVLISIDDYSRKQIANHPELNLGAWPWRRNVWGEVLNYIEQGHPRA
                     VLLDIVFNDISRGYDYDNQFAQELKKYNNVVLATSLNNPKYLSDKIKSDDVVNSFFLP
                     TKKPLDVENFDKQIDYNITYLSHAPIYNIYTEHNTMGVVNKVVGTDSIIRKVQPIFKL
                     EKNKQVYYIPSLAFAGFLKYMGEGGKITIKHNKIFYKNRVIPIDDEGTTLLSWHGKGN
                     DYTYIPISKIFLREKINGKKITPNFFKDKIVIIGRTEAGTDIHASAVNPTYAGPEANA
                     AGIDNFINDTDEKSNLKRKFISQLPPFIKYEIIIFFCLIIAFIGLTSKNAIYALFNSL
                     FLVFLYLFISFYAFAHPGIRLWIPIIEPLFYILLTTGIILALRLHKETTQKREIMNMF
                     GKFVSPNVLSTIIKNPSKLVLKNSKKRVTILFCDVKDFTSLSEKYDPQKLVDNLNELF
                     NEIVNIIFKNNGTVDKFIGDSVMAYWGDPIASPDDAYMAVKTALEIKKKVNEMKLENA
                     KNNKIIFDVKIGINTGDALLGLSGSNKIMSYTAMGDAVNTASRLESACSKLKKDILIS
                     KSTYEKVKEKILAIYVDKIEVKGKDEQIEVYEPVSFVQESLNEV"
     gene            25037..25255
                     /locus_tag="PKI94_00670"
     CDS             25037..25255
                     /locus_tag="PKI94_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25284.1"
                     /translation="MSEKKIKKCPFNSGDCTKECSLFIDTDDLNELLAARLASLGILD
                     KENGICAIKTIAMSTGRFIFENTSTKRF"
     gene            25359..25706
                     /locus_tag="PKI94_00675"
     CDS             25359..25706
                     /locus_tag="PKI94_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25285.1"
                     /translation="MDFTFRFSPESSKGQEAWSNFFLSSDSEETQAAGAIALLDVNDC
                     QGDTFCSKDAFGTTDFSNIGEFVCSADNTSVEGLGSIAFTGFDSGIDCASSAGSFDCA
                     SSDCGASSSSFIG"
     gene            complement(25775..26035)
                     /locus_tag="PKI94_00680"
     CDS             complement(25775..26035)
                     /locus_tag="PKI94_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HNW25286.1"
                     /translation="MYQEEKLICEECGCEFIFTVGEQEFYAEKGLTNKPKRCTECRKA
                     RKQKHKRKLYDAVCSKCGVNTQVPFRPIEGKEIFCRDCFKKD"
     gene            complement(26305..26979)
                     /locus_tag="PKI94_00685"
     CDS             complement(26305..26979)
                     /locus_tag="PKI94_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNW25287.1"
                     /translation="MNLFKKFIIYMIFLGIFPCFAVFGQNYDAEEQININVYEKINPA
                     IVCIDAQLKEGISSGTGCILTQQGVILTSSHVVDGSNDIDVTTISGKVYKAKVLSILG
                     EKNDIALLKIEPQEPLSTVKLGNSEEIKVGQKVLAIGNPFGFSGTLTQGIVSRIDYTK
                     NKIQTDAAINPGNSGGPLVNTTGEVIGISQSIYNPDNNKSNIGIGFAVPINSVKDLIS
                     LKIDKN"
     gene            27127..27882
                     /locus_tag="PKI94_00690"
     CDS             27127..27882
                     /locus_tag="PKI94_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HNW25288.1"
                     /translation="MGRMDLFRQHLKEKRAVKIIAGIDNFDIESIKKVVTAANQAGVS
                     AIDICADTKIISQVKEMTKLPVFVSSIIPKELAQAIESGADAIELGNFDALYKNGTRM
                     GVNEVLDLVRETLSLIDKTQTFFCVTIPGHVSVSEQVALAVKLESLGIDLIQTEGAVI
                     AQAKSEGARGLLEVASVSIANTIEITRNVDIPVMTASGITSTTAPMALAAGASAIGVG
                     SCINKLNSQIAMIAQAKNLVESVNSSQREKVLV"
     gene            complement(27952..29334)
                     /gene="dnaA"
                     /locus_tag="PKI94_00695"
     CDS             complement(27952..29334)
                     /gene="dnaA"
                     /locus_tag="PKI94_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNW25289.1"
                     /translation="MEISEVKKIWEEVKKELEPKIPASSFEPWILPLEPIGYENNQFS
                     VLTGQAFAVSIIRKNHYNQVIEAFKNVLGKEIEFNIIFDEELSQKLKKQIQKAKQTAE
                     KTQSATPMENLAQMQSFCNLNLKYKFDNFVVGENNRFAQAAALTVAKTPCKKYNPLFI
                     YGASGLGKTHLMQAIGHYIIFNHPKLKVRYIKTEDFTNELINNIRKGGDINDRMTKFR
                     QKYRNIDVLLLDDIQFIESKERTMEEVFHTFDSLHNNSKQIVITSDRLPEDIPTLPER
                     LKTRFQMGLVVDITPPDFETRVAILANLADEIGMKISSEVLEFIAHNYCDNVRELEGA
                     FNKVSAFASIQNIELDVDTVKKVLKIDGKKKKVTIENICETVAKYFDISLDELKSSAR
                     NQKISHTRHLAIYLAREMTQMSFVNIADFLNKKHPTILFSYEKIKEELKTNSELEKLL
                     CELKEKIRGK"
     gene            complement(29489..29662)
                     /locus_tag="PKI94_00700"
     CDS             complement(29489..29662)
                     /locus_tag="PKI94_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25290.1"
                     /translation="MATLLLSDTVSGIYKETSEFINSPDLNKKDIRKKSFQELLQESF
                     FHGASRFGGNFIA"
     gene            complement(29792..30679)
                     /locus_tag="PKI94_00705"
     CDS             complement(29792..30679)
                     /locus_tag="PKI94_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNW25291.1"
                     /translation="MNLIISLTIGIAAAIITAIFLYEPQSPLEKRFKRKFKKRESFIA
                     NIIRNLHELLEPLSQKNAKSTIGKTKKLLLQAGEPSSEEDVIKFETQKITGALIAFLI
                     CAVILYIQFDFMVLAGCILFILIVYRLPEIKIKKEIALRRKEFMKFIPDAVDLLSICV
                     QAGLGLDSAFSKVAEEFELTSKTISTEFDRLNKDILSGLSRQDAYKNLLLRNENQDLQ
                     SFVALLIQTEKLGSSIVQSLEAFCDSMRVRKRQKIEEMAQMASTKMTIPMVIFMMPAI
                     FIIIMYPALQKITTNLGGM"
     gene            complement(30687..31646)
                     /locus_tag="PKI94_00710"
     CDS             complement(30687..31646)
                     /locus_tag="PKI94_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNW25292.1"
                     /translation="MIFLILSLIIGISAAIFVWAIFVIEKDLSPNTLKRLTRIKQKTN
                     IIEDDEEEFNLLKVSEYKIQQIGEFLQNFKISNKIKELIALSGVNLYIDTFIFISLLC
                     AAPFLLFILTPFKLMSLFGIVAIFLPTFYLKQTETKRFIEFSKQFPDALNLMASSLKA
                     GHPLFSAIDTVVEEMPKPISDVFETVKKDLSLGIDTKEAFLSMTRIMPKSMDLRFFIT
                     AVLIQKEIGGNLAELLNSLSATIRERFKLIGQLRVQTAQSRLSGIILGIVPIAVLLII
                     SFMNPEYMKPLFETKDGQMAFATAMALIVVGFISIKKASEIEF"
     gene            complement(31643..33112)
                     /locus_tag="PKI94_00715"
     CDS             complement(31643..33112)
                     /locus_tag="PKI94_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNW25293.1"
                     /translation="MNAANKNITVPASSSETPSAASGYGTNDFLSLKEQLHGLLVEKV
                     NSTPTWVNYDDEQQKELIKQFIEGQLLTSFKNVPLNKSEKDRLIKEIIQEAKGYGPLD
                     PLLEDPSVSDILVNGPKSVYIEKNGKLYKTSVSFKDNQHLRNIIERIVSKVGRRIDEK
                     SPMVDARLADGSRVNAIIPPLALDGPSLSIRKFKQDAGSLESLLGWGALTKEISDTLS
                     LAVKARLNIIISGGTGAGKTTLLNSLSSQIPNDERIITIEDSAELALQQEHVVRLETR
                     PANIEGTGQINARDLVINALRMRPDRIIIGECRGAETLDMLQAMNTGHDGSLTTLHAN
                     SPRDALSRLETMSLYSGIDLPEKNIKAQISSAIDLIIQVSRLQDGSRKITSVTELIGM
                     EGNVISMQDIFLFEQTGIENSRVLGYHHSTGIRPKFVEKLKEKNLNIPLEYFNKERKH
                     TYTSSLFTNQVQQAQINKDLSIKSRLTVSNNPLTQRLQK"
     gene            complement(33141..34340)
                     /locus_tag="PKI94_00720"
     CDS             complement(33141..34340)
                     /locus_tag="PKI94_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW25294.1"
                     /translation="MTNQIQTVIIDTQTQSTDVLKLFLKELDYVQILDSFVDVVEGYN
                     YILENRPNLVIVDISNKTELALDIINKISLNHKTCKIIVTSSNYSTDLIIKAMRAGAR
                     EFLPKPLIKEELKTALEKLKEQIAGLVPENNRCRVITVFSNKGGIGKTALSTNLALEI
                     ANITKEKVALIDLNLQLGDITTFLDINPAFDISYVIQNLSRIDETFLLSTLEKYKDTS
                     LYVLADPPYLEQAEDISAEQISTLFSVLRQTFSYIVVDTSASFDGKTITALDNSDLIL
                     LVAIVNLPAIRNCQRCLELFERLGYEKEKIKIVLNRYMENDEIKVEDVEDVLEQKVFW
                     KIPNNYFTIMSAINKGVPVNKVGPDANITQSYKELAAMLSDNIYKHSFAKKVERKPFF
                     DFKSLFK"
     gene            complement(34337..35644)
                     /locus_tag="PKI94_00725"
     CDS             complement(34337..35644)
                     /locus_tag="PKI94_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HNW25295.1"
                     /translation="MKKSLILLIILFLMSILTAKLVKAAEPDYKDTPIPQQLGTVKSD
                     LVKDSVNLNPYKDSNEKLYSIVGKTQLIKFDEPVKRISITDPSLADLVLLSPKELMLN
                     GKKGGRTSLIFWGDNNKPVFFNLVVQQDSDAFMQAIEEVAPNEDLQYTFTDDGVILSG
                     KISSTTVKENINNIVKAYNFKLLDLTESPTKQVLLEVKITEASRNFTRELESKFTVGE
                     NYDNFLTQILGITNHQDLSNATVNGNTGGQYIYSSSTARVAWALRMAEQKGIVKILAE
                     PKLLAINGKSGSFNVGNEIPVPSQMGQYGNIAYDFKTTGVILNFTPTILEKSNRVMLK
                     LSPEVSEIDSSYVSQSGIPGFKTRKVDTTVELGDGETLVIAGLLSHTSSQSKSKVPWI
                     GDLPGIGLLFSSLKNEKRDTELVIFITPKIVEVDKDNHNRIDL"
     gene            complement(35656..36522)
                     /locus_tag="PKI94_00730"
     CDS             complement(35656..36522)
                     /locus_tag="PKI94_00730"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNW25296.1"
                     /translation="MRTNKNTSKIILAIVVALLATMISYSSFNNMQAQLGEKEKILET
                     LQGSDTIKDFSYAVATKDLKAGELVADDDIDFSSFSTPTFGAFENRSDVVNKVLLKDI
                     IKGTVFTDSYIAKISGDEIELKEGFRALTLPAEKFQGKSNRMKTGSNVDILSDFEGGW
                     SMEGVKIIGLEGEKGLPAGDIISAKTITFEVPADDMADLIMSISKGNLILVSRNPGDK
                     KIGLKKKKSPASTYSANDSYSSLPNLPANIPISDYPSSGVSGLPQPIAPLSNAPSVEV
                     IEANVKTKVTFD"
     gene            37000..37632
                     /locus_tag="PKI94_00735"
     CDS             37000..37632
                     /locus_tag="PKI94_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25297.1"
                     /translation="MIKRVFVELKEHAPFTFLGALSGILIFVLLKNIPYKISYGLFYT
                     FHPLHVFLSAYVTASMYKLHKKNPDMIKLFLIGFFGSVGVATLSDSVIPYLGEILLKM
                     PNTELHLGFIEKWYFVNPVAILGIVTAIFVSKTKFPHMGHVFVSTWASLFHIMMAGEN
                     MGSGISLYFWILILLFFSVWIPCCFSDIVFPLLFIKGHDTDCPCTCCGKD"
     gene            complement(37683..37771)
                     /locus_tag="PKI94_00740"
     tRNA            complement(37683..37771)
                     /locus_tag="PKI94_00740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37735..37737),aa:Ser,seq:gct)
     gene            37847..38617
                     /locus_tag="PKI94_00745"
     CDS             37847..38617
                     /locus_tag="PKI94_00745"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNW25298.1"
                     /translation="MTLKIADKEFTSRFMIGTGKYPDFEVMQKAHRASGAQIITVAIR
                     RVELNAPGHVGLMDYIDLDKYWILPNTAGCATVEEAVRIAHLSRAMGINNWIKLEVIP
                     DSKYLLPDPIATLEAAKILVQEGFVVLPYINADPILAKRLEEIGCATVMPLASPIGSG
                     QGVRNLDNIKIIIENTNIPVIVDAGLGVPSDASTVMELGADACLLNTAVALAQDPEKM
                     AYAFKLGIEAGRASYEAGRIPIKEIASASSPLVGVVGK"
     gene            complement(38619..40427)
                     /locus_tag="PKI94_00750"
     CDS             complement(38619..40427)
                     /locus_tag="PKI94_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25299.1"
                     /translation="MQIIEVKNNYVKISYDASAQELVLSGFLVIKDRIQSFIAQIMHL
                     EANSEGSFAVAKLLFNFDDQGTVTGYNGAIPSLSSPVGMVYPQEILELFNTQNPILLG
                     ELAQQKTPLKLDYEFLKEKLLICCEKISDSRLLTENLNAQLCALGKKVLVFDFAGNFD
                     FSKNRLTAGKSFKLPLNSEAINFIYEKGLTDAKAETKALLQEVFLELQNYVKTIPDMF
                     VPFETFLKVVDEQYRQLGYIELVLLKNRLLRFFEKGVFAQQKKDFAKLKASLKHPVTT
                     IFNLSKMDEMIQREMISYAYSLIKTLDKEVYVIVNLGENSDKKLLKQIFTTKNAFTTI
                     ICSYSYKYLNELKQIAKNLFLFAPIEPQNYFAGYNTFLNKLNAGEFIVYGSATNHIPF
                     IVKLSDLPQTNFESPVVTPPPPTPEQVAKQEIVEEQIKKEVDEIYTRPKSEINTPEEP
                     VVTTIEDLSDEDLDFIENSITNPAEEDEIIQESISPLDVIEIAPQQILEQEIPPQPVF
                     ESQEKEEQDEILNVSTQNEGGILPVYSAEIQSENPLGAQIFEQGETVYHPKYGKGTVE
                     KIISYGTKSLCSIQFENVGRRLLDPALAEIRKISEQ"
     gene            40604..41101
                     /locus_tag="PKI94_00755"
     CDS             40604..41101
                     /locus_tag="PKI94_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25300.1"
                     /translation="MVTQELEKTVLSPHEVRNILNADNREIIRLCKKATIIPKKNKQG
                     QTYFTSDEVKRLKRLQNEVPAVVTSPITQAESNLVVANLLSTLEKFENNITDSLSKVI
                     EEKLEGMDEVVVELIRCKTENESLRQKLNELNKENYNLKNSIKSYKSVGLGFYIKTSQ
                     DDFSI"
     gene            41207..42226
                     /gene="recA"
                     /locus_tag="PKI94_00760"
     CDS             41207..42226
                     /gene="recA"
                     /locus_tag="PKI94_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNW25301.1"
                     /translation="MVVKEQEIKAISEEKNKALKMAIEKIEKDFGKGSIMKLGDRPMV
                     NVEAIPTGALALDVALGVGGIPRGRIIEVYGPESSGKTTLALHIVAECQKKGGIAAYV
                     DAEHALDPEYSRNLGVNVDDLLISQPDTGEQALDITEELVRSGAVDIVVVDSVAALVP
                     KAEIEGSMEDQQMGLQARLMSKALRKLTGVIGKTNTTVIFINQLRMKIGVMYGNPETT
                     TGGNALKYYASVRLEIRRTEGLKKDAEDIGNHVRVRVLKNKVAPPFRQAEFDIIFGKG
                     ICKIGNIIDVAVNLDIIKKAGAWFSYNDEKLGQGRDKAKELLESNPALLAEIETLVRE
                     KLAQQ"
     gene            42414..43865
                     /locus_tag="PKI94_00765"
     CDS             42414..43865
                     /locus_tag="PKI94_00765"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNW25302.1"
                     /translation="MIKFDLFTLEAFVSENKDFLINSRIQKIQQPTRKELILHLRNQG
                     ESRKFYVNTNPEFYHVCFMSKENEQKRLIEIPKAPPMFCMLLRKYLEGAKIAKVEQPK
                     SERILEFYFEVYNEISEKIYLCLAIELMGKHSNVVLYNHDTNVIIGCLHNVGSDKSRQ
                     RELVGGLPYVYPPKSSGGKFLCLADNFDKKLSVNENIDNYFANLQGEKKFKTLKSKLF
                     SYFNSDLKKINNSLLKQQKQISSDEKTQTYRKKADLIMSGLNEIKNKDFLTFIELIDW
                     ETNLPVKIELDKSLTLKENANRYYKLYNKAKTAHSKTEEMTAELKREKDYLEQVLYSV
                     ESVQNIDELLEIEEETEQKRGQEGKKAGGQEKKIFSPIYPLTLHSSPFTIYIGKNNKQ
                     NDYIVSKLAKDEDLWFHVKDCAGSHVLLKTEKQIPSDEIILKCAKLAKEYSSAKNSTK
                     AAVIYTKAKYLKKPPKAKLGYVTFTNEKEIVID"
     gene            complement(43862..45103)
                     /gene="hisD"
                     /locus_tag="PKI94_00770"
     CDS             complement(43862..45103)
                     /gene="hisD"
                     /locus_tag="PKI94_00770"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNW25303.1"
                     /translation="MQIIKYENLTDNFFEKIKFEAISSVDEIAQEVRKKGDSAIRKFH
                     AQFDKEILDGFLVTKEEILEAYEKVDANTIKAIKKSIKNVETFAKQQLKSLKSLKTTI
                     DKNELGHKIIHLEKVGCYIPGGNYPLISSAVMTIVPANVAGVDEIVACSPKIKPEVIV
                     ACDMAGADKIYKIGGIQAIAAMAYGTQTIPKVNKIVGPGNKYVTAAKKYVYGEVGIDF
                     LAGPSEIMIIADESANPEFIAADMLAQCEHDKDARAYLICTDKDFAKKVEIFAQKKLD
                     ELSTKEIAEKSFEKSYAVIVKNIDEAIEIANKKAPEHLEICFKDAKKYINKFKNYGSL
                     FVGNLSAEVFGDYCSGTNHVLPTNEVSKYSGGLSVFDFIKIQTYQIISPQGKKLISPC
                     ASKLAQMEGLAAHKLAADIRN"
     gene            complement(45228..46457)
                     /gene="hisIE"
                     /locus_tag="PKI94_00775"
     CDS             complement(45228..46457)
                     /gene="hisIE"
                     /locus_tag="PKI94_00775"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNW25304.1"
                     /translation="MLIPSIDLMNGKAVQLKQGKEKVLEREDVFELAKYYSRFGDIAV
                     VDLDCAMGNGNDNEELIKQLCKIASCRVGGGIRTVEKAKRLIAAGAKKIIIGTAASEE
                     FLSQLPKDRVIVAIDSKNGRITTEGWTKELDKTPLDFIKHFDKLCSGYLYTVVEKEGM
                     MQGCDMASIEELKKATKKEFVAAGGICSIEEIVDLNRKNISTQLGMCIYTDTVKLEDA
                     FLACLDFEKGKGLIPTIVQDIDTKQVLMLAYSNEKSLRKTFKKGLATYFSRSRQELWT
                     KGETSGNIQELQTVRFDCDFDTLLFKVKQKGNACHLDRFSCFEDKEFSVFELFELLKL
                     RKENMPESSFTTKLFKDKFLLKRKIMEEAFEVVNFEQGDGLGWEVADLAYFILTFMAE
                     NNVTPQEILNNLASRSK"
     gene            46540..47499
                     /locus_tag="PKI94_00780"
     CDS             46540..47499
                     /locus_tag="PKI94_00780"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNW25305.1"
                     /translation="MKAVVFDGELKLVTDYKKPEPKVGEALIKVSMAGICNTDLELTK
                     GYMGYKGILGHEFVGVVEEVNDKDKSLLGKRVVGEINCGCNNCEWCHKGLQRHCPNRQ
                     TLGIWQKDGCMAQYVCMPLDTLLEVPENVSDEQAVFVEPLAAGLEILEQLHIQPVEKV
                     LILGDGKLGLTTALALNVSNLDVLLVGKHQNKLDIAKNQGVNVKLLSDLVIKKEWDFV
                     VEATGSISGFETALNLTKPRGTLVLKSTVAASKEFNLAPIVIDEIKVQGSRCGQFAPA
                     LRLLKSEKIDFSPLISAVYSVDKALEAFEKNKEKDTLKVLLRF"
     gene            47706..48824
                     /locus_tag="PKI94_00785"
     CDS             47706..48824
                     /locus_tag="PKI94_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNW25306.1"
                     /translation="MGVNFIKRLTILDKYILRQVIEMFIMGVFVFTSIIFASDTFITL
                     IKQISLYGIPFKVALMIIILNLPSVFVMTIPMGVLLATVMTLNRLSLASEITVMRSCG
                     IGINRIAKPIFIFAAIMCLASFAINETVVPVMTKQSKDLALWALGQKNIPEGKQNFIF
                     KELRDNGTLKRLFYVGYCKDKVLHNITVLDLGKDDTIQVLQAQDGGTSPSGWEFNRGA
                     IYTVAKEGKILNTTFFEHSIAQFGIDLSRELNQNVAKEHNFTSLIKYMIENRDKISEQ
                     KNFYLIQLFDKLALPITTIVLVLIGVPLAITPPRVRYNRGFLFSILIIFTYYLIRALS
                     LSFGEAGSLHPFIAAWAANILLTLAGVALYYRKVYTIN"
     gene            complement(48821..49114)
                     /locus_tag="PKI94_00790"
     CDS             complement(48821..49114)
                     /locus_tag="PKI94_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25307.1"
                     /translation="MSNSVNTSNYGQFGLTWGSMGQGFRAFGENIGARVSEALEKPNE
                     ELNNFFDQVEFVQSEDYEKLREYYNIAAFNTTMAMNRENLMTNGKTPKLSIKG"
     gene            complement(49220..51253)
                     /locus_tag="PKI94_00795"
     CDS             complement(49220..51253)
                     /locus_tag="PKI94_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNW25308.1"
                     /translation="MISRQKITLIAIIIFAVIAIAFINTPKIWTPESISNKKYTQALE
                     KYKNNDLSQAYADFEKIPKTSKLKPAALYRQAFCAEQLSDKKGAIKKYNEIIKKYKNS
                     KIALKAKYSKAQYLYEEKSYKKAKKEFKEIIRKYPQSDYALASGYYMGSIETLHIERV
                     KNKKKKNKTIKKASHYFRNYLKEAPSGRFSINCIEKWILLNKKLKNEDNLIIAKSYMA
                     NGDFKNAQKYLNYTNINISWPYFVQNAYSMKDYSKVKYYTQFGLKEPPVNAVAINEAP
                     EDTIDNKAVYDAIDTYLSISQNPKTSISYLLSLSENAPGHDYLLYKNCLNMPQNLQLA
                     CFNTLYRKYPKGQFAAESLSNIFYEKIKTRDYYTAKKIGSEHLSNFPKTNSTPRVMFW
                     LAKINEKTKNYDIAINFYKNLILQFPDDYYAYHSYLNLNGFKNLSLTAEELENKEILF
                     PYKKNSENELIIKLAQVKDWGLINELCKDDDFIQSWLKYQQGDFSSSARIARDAMDKL
                     ETKPDRKDPRWRLVYPIHFYKEIKENAKIFNNNPVLILSIIREESYFNPKSISPVGAM
                     GLMQLMPITASEVGKDRGINLLSDKFLLQPDINIKIGNIYYSRLRKVLFDKDILAVLA
                     YNGGIGSVSRWKEDINFADIDDFVEQIPYLETQNYLKKVYKSYWNYVRIYSND"
     gene            51353..51499
                     /locus_tag="PKI94_00800"
     CDS             51353..51499
                     /locus_tag="PKI94_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25309.1"
                     /translation="MSEKIFKTDSKTAQKSKYFSMPRHDFVFQSRKNLIKNYILDPEV
                     KLVK"
     gene            complement(51502..52122)
                     /locus_tag="PKI94_00805"
     CDS             complement(51502..52122)
                     /locus_tag="PKI94_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNW25310.1"
                     /translation="MLNDLKNEIIKYGDIVDAKGFSPGASGNISVRYKDKIIITASGS
                     SNGDLAWEDLVVIDFEGKVIEGDKKASSEKMLHVAFYKKRSDINAIIHVHPSYLSSFA
                     AAGKGLEEPVMAENVYYFGKIPLAPYALPSSKELVEKTEKYFEKHNAVLMANHGFIVG
                     DINLKQAYLKLELAESYAQVVLNAKLLGGAVLLNKKEVEEIEALKI"
     gene            complement(52140..54263)
                     /locus_tag="PKI94_00810"
     CDS             complement(52140..54263)
                     /locus_tag="PKI94_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25311.1"
                     /translation="MKKNLILLLIVLATSSGLAFAGQEPKVISDIDSKKQTMDSFSEI
                     NSYTNNFFTSVITQQEKERLKKLKEESISKTDQVPGEGQMRGTLPPVKKIRLAIKNKG
                     KKTQETSAQTTASKNQALLDCNTMEYFADRTELEANGNVVLFFPKNNSTIKADKMIYN
                     QTSNLIKAFGKVVLISDGKELTGDYMQVDLNEENAFLEKPEGDIFQIQAKAKMGYMYG
                     DKIIQEQGSLYIKKKMPINLRSDMFGPDLSTMYLANQDKALYKKDSHGEKFKIKTNDL
                     IINSKDEHDTVTLKHAEIYFDEKKIGTIPSITVHTNKNQDYVEADYPELGTTTNLGFY
                     AGPGFVFDTPKGSTVKLIPMFNYNAGKIGAGGIAKFKNATNKTDIAYGTAGEMFIVRG
                     KQRLDDNLYLQYGSNAYMDDWFMGFRMPKYMAEVYYDDRVLNENFLGKNLDMMFSQRF
                     AAGYMQDGDVGDGNIGALKSEGGIGTLRLKYMTEVSQTIYNFNCERFMQDASLKTIKN
                     KDDILAARLEMVFQGAASLYGTGDTQMLARVGPRLHTQYKYWMQDVGYFLSGYNDNTP
                     MPLFDKYMYGRSNAYVRETLRLTKYLTLSWFGSVNLTNDAWNGSLMQENSFFFSLGPD
                     DIKLHVGYDTVRQQSFVTMALALDAKGSTIEYKRMEIKNPDTLGKQKNGKNNPNNTFA
                     PTTQDDDSGIERADVIEIKNDNETI"
     gene            complement(54273..55388)
                     /locus_tag="PKI94_00815"
     CDS             complement(54273..55388)
                     /locus_tag="PKI94_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNW25312.1"
                     /translation="MNKNIFKIFAIFFFATAMLSFVAMPCFANTELQQKQQQTRAKIN
                     HLKWLENLETNKLYKNQQKLERTTSSLVESRQQLNTTQSKLSNLETDLSRSLSDFSEI
                     EFQMKRRIRQIFKSQRGGFFELLLSAQDFNSFLDRLYFEGIVIRGDYRRMNYARMKAK
                     EIALLKYNIEEQKRVLSRSIKNINYQQQAIQQDIARNENMIHRLRTDRRTYEQAEREL
                     ARQSESIQAMISRGSHASDIRIVSGFIKPISGRITSPFGWRRHPIFNSRSFHSGIDIG
                     GPYNGSIRASNAGKVIYTGWYGGYGKVVILDHGQVNGTPTTTLYAHLNSSRVSVGEYV
                     SKGQVVGYEGTTGYSTGPHLHFEVRINGRPNNPLNYI"
     gene            55891..58317
                     /locus_tag="PKI94_00820"
     CDS             55891..58317
                     /locus_tag="PKI94_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25313.1"
                     /translation="MKYNFLNIFLDKEDLFNQEKFSIDKFLLENNYSQIEKIYNFYKE
                     TSNLLYVSGFLGTGKTQVINYTASFLSAETVLLSYFCSSSTILDDILLSFFAEFRRLA
                     DKKLIVEPKVKTENFAEKINVYFSQIERPFVIILDSFDSILSENRQEILDFIMHLCKL
                     NKVKAVIISKTFEQEYFNNPSVRLEKVALSAFSQGIFEKYLKSEGIKLPPKILEEFYK
                     QTRGYMFFTSLVINLIKSKNLEPIQFLREFSESFLTLDEFLSRQTLGFISSEAKNLFW
                     FLSMLRHPVNIELLKILDVYDEQKMKNLKDNFILVKNNSSVYIHEFFKDRFDIEPPAN
                     IVQKMHQYIVDIYKTQLPLKPFERNILISRQTMRKEIKYHEFFLPKKINTLEDKNIDI
                     GYLSYAKGLEFDLGHPTSASSQSVVSAPFSETKKEVEVKTFNPEAKDKTRIVSQIFPH
                     EKLQKAEEVKKISISDLIKNIKDAKRAYNYGLVIDFCKELLLREDTLENKKDLPFIYT
                     ELAFAYQKMADIENALKYFNLAQEKFLQQGKTTEANEIKLTISNIFIENYKTEQAKFI
                     LEDILKCEEITDILRIKALMHLAVVEDNLSNTQKTFEYYKQAVKISKDVIDNNVLMEL
                     YFRFAAFLDDNNETQLAQEYYLKCINLDLESDNNFVALANSNLADLYLEQENSKSARE
                     HYFRAFEIYNKNGNYDGLYHSSSKLAVILSIRSPQETLKYLEAAFESASFLNDKFYMA
                     SAQLALGDFFYDSLNDEMAIKNYLGALKIIEKESDKENSDKIQLRLKDVKLRIGEEKF
                     IELINRFGNE"
     gene            58310..58933
                     /gene="rsmG"
                     /locus_tag="PKI94_00825"
     CDS             58310..58933
                     /gene="rsmG"
                     /locus_tag="PKI94_00825"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNW25314.1"
                     /translation="MNDFGEKTAIIEKYARIFLEKNSRLNLISKNDEKVFLKKHVSDS
                     LAIDKFFQKHKIKKGQKLLDMGTGGGLPAVPIAIFYPVLEVFAIDSISKKINAVEEIK
                     KELELENLYPIWGRVENLPAQNPEKFDYVVTRALAPLDKILKYAIPNLKTGGYFIAYK
                     SKRALDEIKEAQKTLKKYNLKVVEIIEYELKSEQTYQRNLIVIKVSK"
     gene            complement(59146..59307)
                     /locus_tag="PKI94_00830"
     CDS             complement(59146..59307)
                     /locus_tag="PKI94_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25315.1"
                     /translation="MILKPVKKEVEEDATDKIQEEKVVQAPFVDRSEDLFEYMEMIAF
                     NNRSILKIA"
     gene            59475..61370
                     /locus_tag="PKI94_00835"
     CDS             59475..61370
                     /locus_tag="PKI94_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25316.1"
                     /translation="MTYPINTKLNQYPQGFSATPAIPVDAEKVKQSVNQNGLVKQAKE
                     EENPVLLLGVMMPIWAAIGHLMAKFNYACSDANGKNILQKIADFGHNIGENKVFKSPG
                     AKHAGNIFSNIKNFAKTKIIDKSSILSAMFKTPTAPEFQMAKVMSKGTISEVATDASQ
                     ALDVFVAQHNSLDDALKALKIDKVLYENIKKDPYKHIDDIIDICKKQKGDAFVMLKKL
                     GRMPLPKFLQRKVYFSELANKLTFLKGLKNPNPNLNVVGKVLPKMSIRTLEGFTNGTA
                     GGKFAILMQAYIFASALIKAAKAPKGQKVKTFSENVFYDLGFYLTIPSSMALMHGAGG
                     LKYIGMSEKQVDFYRKALEKLNASVDAGTISKVKYLKARKDLGILLKGNTKILKSDKF
                     LVRSAKHLKNIVYNPLKWAGKMVSVGLERIKPYAGKDANAILKGFGKFKYKLKGAAGV
                     PARFLLFSMVFAPFLAKYFAKASHLIFGRPTKSVLDDDKESKAPEQIKSQPTNQQGAV
                     PTSLITPPAQSGNLVNMYNQQANKPVPQAVQPQQPASQQQIAPGQALQPVVQPAAVQP
                     NTETSLVYPPKTANQTPPAPARTYVPSAEPIKQEKTQQMTEAEQKLALALQKADSAEQ
                     YAKKYAG"
     gene            61583..62452
                     /locus_tag="PKI94_00840"
     CDS             61583..62452
                     /locus_tag="PKI94_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25317.1"
                     /translation="MNTILNTDLDTAQRNCVASPIHKNRFQKLETALRDLFYKELNSS
                     DSIFLEISPLLVKKVAMFLSGLAFRPAAIGICGETASGKSTIVTDSIDVINSFAQEFI
                     LENVVTTINTDDYYYDRSKEVQKAGSFAEFVKTYDLDVPQAMELSLMKKHIQALLSKQ
                     SVLLPKYDMSGTAKRFENHTKANPCSVVISEGLFTLTDEIADVFDFKIYVDIDKSVQK
                     RRFYERAQKRGLGDSADRIFQNATDKAEIYIRPCMKRADIVLNGEISRQKYKQFVASF
                     LNCLGEIYYGIIM"
     gene            62475..63068
                     /gene="nadD"
                     /locus_tag="PKI94_00845"
     CDS             62475..63068
                     /gene="nadD"
                     /locus_tag="PKI94_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNW25318.1"
                     /translation="MKLCVFQGTFNPIHSIHMAMAGFVKKVYNFDTILFIPAYKPPHK
                     EFDDDLANHRLQMVKIAIEGNTSFSISNIEFQNERYSYTYLTIEELYKRYKIEGKIAF
                     IIGMDAFREVTEWYEADKLKTLVDFIVFPRGQEIDKDRLALLRYKGYNFTIAKMDAVD
                     LSSSMVRERIVKGQSVAGLIPLKVMEYIRENGLYKQE"
     gene            63046..63630
                     /gene="yqeK"
                     /locus_tag="PKI94_00850"
     CDS             63046..63630
                     /gene="yqeK"
                     /locus_tag="PKI94_00850"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNW25319.1"
                     /translation="MDYTNKSDKEILAWLEKNLSQGRFLHSLGCAKRSVELAKKFGQD
                     EKKAYVAGLLHDCAKSFDVEEMRKIIQDKKLDVASCEFVNPKTLHAPLSAYVAKTAFG
                     VEDEEILSAIRWHTLGRVNMSKFEKIIFLADKIEPHTRKTEYAQLIMGILEEENGIDK
                     ALLVCYGETIKSLKARGLEICQVTIDVYNSLQSL"
     assembly_gap    63685..63784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<63785..64938)
                     /locus_tag="PKI94_00855"
     CDS             complement(<63785..64938)
                     /locus_tag="PKI94_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNW25320.1"
                     /translation="MSGEREAVAIIGAGPAGCMCAYFASKNADVILFDYAQPLHTLLY
                     TGGGRCNLAYAQYDFKELAKNYPRGEKFLYSVFSQFSTSNAIEFFEKIGVKTYTQQDS
                     RIFPISNSAKEVKEKMLKSIKNCTIKKEKVIKIKPLTLPSPYGTPHPNPLPQGERESN
                     YNKIFSPRGEGKEKSKFLITTDKNNYSFDKVVIATGGHAGFDLAQSLGHKIIKPKPSL
                     VGLISNENFSSLKGLSLKNIKAQVFFNDRLITKENEDILFTHQGVSGPLIYKISSLCA
                     RLDYNRQNPLKIKLEFIPEDFDLQIALNSNVKKNIKNLLCEFVPKSMAEYILGLNNIK
                     VDDKCSQINSQKRDLIKKSLSEFEINAISPTKEGEIVTSGGICLDEINSKT"
     gene            complement(64949..67768)
                     /gene="ileS"
                     /locus_tag="PKI94_00860"
     CDS             complement(64949..67768)
                     /gene="ileS"
                     /locus_tag="PKI94_00860"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNW25321.1"
                     /translation="MGKEYKDTLNLPQTTLEMRANAAQKEPLIQKKWVEENIYDKIIE
                     KKDKSHKFVLHDGPPYLSSDKIHIGTALNKILKDILIKYKTQAGFYSPYVPGYDGHGL
                     PIENAVVKNIKGGREALSPFELRQKCREFAHKNLKGQEAEFKKLGVLGDWENPYLTID
                     PDFEAEQIRVFGQMYKNGYVEKGLKPVYWCAACETALAEAEVEYADHTSTSIYVRFKF
                     DEESINKIQKIIPSLFTVHRSLYAVIWTTTPWTIPSNMAICLHPKLEYTFFDYKNDVY
                     VIAQALLASFLQGVGWEEKDIKVLGSVLGEELELLNTIHPLYERKSPIILGEHVTTDA
                     GTGAVHTAPGHGLEDYEIGVKYNLEVFSPLDGRGIWTNQVPDLEGIPYYKGNAIVIER
                     LEQKGALLAKSEIIHSYPHCWRCKKPVIYRATPQWFVKVDKFRNETLEEIKKVKWIPA
                     SGENRIFNMVEGRSDWCISRQRAWGVPIPVFYCKDCGEVIVTDETIKHVAELFDKESS
                     DAWVKYSEKELLPNNFKCPNCKGENFRKEKDIMDVWFDSGITWRAVVEKRQKELGNIP
                     VEMYLEGSDQHRGWFQSSLLTAMATQSKAPYQEVLTHGFVLDAEGRKMSKSLGNVVAP
                     QEIIKVYGADVLRLWAASVDYRNDIKIGENVIKQLVEIFKKMRNTMRFLLGNIYDFDP
                     QTDYVQYSELKNLDKFALHKLNRLTKDVTEAFDSYEFYKYFQYLQNFAAVDLSAFYLD
                     IVKDRLYTAGKKSLSRRACQTVLFEISQALVRILTPVMPHQAEDIWTSVIECQKDGME
                     SILLSNWVKFNPRWENAQLEEEFATILKAREIVTKAIEPLRADKKVGSSLEVAVYVKA
                     PQESHEILSANQDELCNIFITSQAFLTEQKPQNILNEYKEEGFFVWVTQAQGQKCERC
                     WKYRTLGEHEGHETICSECVDAISE"
     gene            complement(67790..69055)
                     /locus_tag="PKI94_00865"
     CDS             complement(67790..69055)
                     /locus_tag="PKI94_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNW25322.1"
                     /translation="MRKSKFTLAIFMALVLGVFVGWLFPDFAQKLHILAVIFLRMVKM
                     IIAPLLFATLVVGVAGHGDVKSIGKIGLKTLVYFEVVTTLALIIGLSVGNFFKPGEGF
                     GVTINPSSLRTATEMAAVHVHSSFHELITEVFPTSVIQSMAEGNLLQIVVFSIFFALA
                     LCAVGQKAKPVLDVLNSLSEIMFKFTEYVMYFAPVGIFGAIASTVGENGVRVLLGYAK
                     VIFALYFALAIFVAMVLFIACKIARISLRNLIKAIQEPALLAFSTASSEAALPKAMEI
                     MEKFGVPKNIVGFVMPTGYTFNLDGSTLYLALGVLFATQIVGIHMSFNQQIMIMLALM
                     LTSKGIAAVPRVSLIVLAGTLASFNIPLIGVAILLGIDQILDMGRTTVNLIGNCVATV
                     VIARWENDFDYDKMREFVREAKIKKMQPA"
BASE COUNT        21365 a        12118 c        12227 g        23668 t
ORIGIN      
        1 attctttggg ttttcttcct tcaagaagtt cttcaaccct tggaagaccc tgtacaatgt
       61 cacctgtttt ttgtctttca aataccaatg tagcaagcat atcgcctcta agcaccaacg
      121 cacctgaacc gacttgaagc attgttcccg gagaaattaa gtaaggacgt cctgttctta
      181 caactatttc tgtttttgaa acttttacaa ctttacctga tacattagat ttttcaccgc
      241 tttcagagag cactgaatct aattttataa aatcaccttc tttaacgcaa ggttttgttt
      301 taataggaat ttttacttca taattatcgc aaatcaaaag cagtcttctt gcatcttctt
      361 ttgctttttt aattgaagca acaccgtcat tgattgcaag aacctgagtt tttgcgacag
      421 gtgtttttgc agcgatagtt tgaccgtgtt ttacaagaag ttctgtttgc atagaagaag
      481 ataattttga acttccttca acttctcttc ttacaataag gttttccaac acaacaactt
      541 taagattatt tttgctgtca agttccacca tacctttaag gtgtgaaaga tatccctgca
      601 tttgaagcac caaacttgtt cttgtcaaag tcgccccgtc aagccctcta actcttgcgc
      661 cgtctttgta ttgaagctgt gtaacaggaa ctatgtcaat caattcttct gtcgtattga
      721 attttattga aacgtctttt tgtttaacgc tcataacttg aaccggtctt ataagaattt
      781 gaacaggctg atttgcaata ttttcttcat ctaaatgcaa acttgaatca agttcttcat
      841 ccaaatcatc aatgtccaaa tcgtcaatat cggattccaa aattgtcact attgaagttg
      901 cttttgcttt tatgccttct gcgataagcg ttcctttttt aacaatttct ccttcttcta
      961 ctttcaattc gcttacactt gaaagtgtat gaatttcacc cggtcttact gtaatttcat
     1021 gaattacatc gttatattct ttcaattcta caaacccgtc aaggtgacag taaatgtctt
     1081 tgataatttc tgttcctgcc gtaacaaaag tacctgattc aaccatctta aggtttgcat
     1141 ctttgtttat ctggtgaatt tcttccggta taaatactat gtcgccgggt tttgtaataa
     1201 tttgattgtc atcaacttca acatcaacat atcttatttc accgcttgat ttaacagaac
     1261 attcgtcatc tattaattcg gcaataatca tgccgttttc tactgttgta tcaacaggag
     1321 atttaacgat atatttttcg cctgaatctt tgactgtcca aatttgttct ttctttgttt
     1381 gttcaaaagt agcgtttgaa ggtattaaag aagcaattac tattgtaagt tcttttcctt
     1441 gaactatttt tttaattttc tttccttcaa attgaacttc ttcaataacc aactcttgtc
     1501 caactctgac ttcaccgcca tgctctgata tagtaagcgt ttcagcaagt ttttcgcctt
     1561 ttgaaatttt ttgttcatct ttaacaagaa cttttgagcc gccaggaagg ttataaacat
     1621 ctccgcctaa cacccaaata attccgtttt tatttgctgt tcttgaaacg ttaccctgtc
     1681 tgtctttttt ctcgtcggct acaaaatctt caaataaaac ttcacccgat aaatcagaag
     1741 taatatcttt tgtggctttt tctgtcaaac ggcttccgtc accttgactt ccgggttcaa
     1801 attcagctaa tttatcgcct tttttaacta ccattccatt tgcaaaaaat acagttgagc
     1861 ctgcaggaat atggtatttt gttgctcctt ttgctgtttt aatttcaata tcagcttcat
     1921 aagttgcgac ctgaacaaca tccccgtgcc ttgttcttaa ttctctggtt ttaactttag
     1981 agataacttc acctgcttct tttgccataa tttctctgtt agcaccggca cctttaaata
     2041 caccgcctgt gtggaatgtt ctcattgtaa gttgtgttcc cggctccccg attgactgag
     2101 ctgctataat accgattgcc tctcctacat caaccatttt ttgagtagtc attgcccagc
     2161 catagcattt ttggcaaatt ccgtattcaa gtccgcatgt aagccctgaa cgtactttaa
     2221 cctcttgaag atctaaagct gcaacttttt taacatcttc tctgtttaaa gttgtgccgc
     2281 aaggaatcaa aacctttcct ttttcatcta aaatatcttg tgctattgtt cttcccaaaa
     2341 gcctgtcggc aagaggagca acaatttttt caccatttga aatagaagtc ataataataa
     2401 atttatccgt atggcagtca acgtctctta tgataacgtc ttgcgcaacg tcaaccaaac
     2461 gtcttgtcaa ataccctgaa tccgctgttt tcaatgccgt atcaacaagc ccttttcttg
     2521 caccgtatga tgaaataata tattctgtta cagacaaacc ttctttaaag tttgatttga
     2581 taggcaagtc gataatctgg ccctgcgcat ctgccatcaa tcccctcata ccgacaagct
     2641 gagaaacctg agataagtta cctcttgcgc cggaaaatgc catcatataa acagggttta
     2701 atctatcgaa attctcgaca acgctttctg taagccttga agttgtttca gaccaagtat
     2761 cgataacttt tgtatatctt tcaacttctg ttatctcgcc ttttaagtac ctgtgggttg
     2821 atttttcaat ctctttttcg gcgtctttaa gcaaatcttt tttcgtttca ggaacctgca
     2881 aatccgcaat agaaattgtt gttcctgctt ttgtggcata tctgaagcct aaatttttaa
     2941 ggctgtttgc aagagttgcc gtttttgctc cgccaaactc aagatatatt tgagacaaaa
     3001 gattgttcaa agactttttg tccataattt tgttgatata atcaagagat ttttttgcgt
     3061 ttttatattt tgtttccatt tattgcacac tccttgtttg tgtttgttct tttatttttt
     3121 aagtaagcag taaatgaaag gtactctatt tactatttac tatttactat ttactaacct
     3181 attaccgctt ttctcacagt ttcattaaaa ataattcttc caactgtggt ttctaccctt
     3241 actccattgt catctcttac aataattttg tcgtgaagtt ttataacttt agcctcaaga
     3301 gcagaaattg catcctggaa attgtagaaa tgtcccttaa caggagcgtc ttcgtctttt
     3361 aggattgtca aataatacat tcccaaaacc atatcctgag aaggtgttat aatcggcttg
     3421 cctgttgcag gggcaagaat attgtttgtt gcaagcatca acattcttgc ttctgtttgg
     3481 gcttcaatag aaagcggaac gtgaacagcc atttggtcgc cgtcgaagtc ggcgttaaat
     3541 gccgtacaaa caagcggatg aagctgaatt gctcttcctt caaccaatac cggttcaaac
     3601 gcctgaatac caagtctgtg aagagttggt gcacggttca gcattacagg gtgaccgtcg
     3661 attacctctt ctaagatatc ccaaacgata ctttctgaac gttctatttt ctttttagcg
     3721 gatttaatgt tttgaacgta tccgcgctcg attaatttat taaccacaaa aggtttaaac
     3781 aattcgatag ccatttcttt aggcagtccg cattgattta aactcaactg aggacctaca
     3841 acaataacag aacggcctga gtagtcaacc cttttaccca aaaggttttg tctgaaacga
     3901 ccttgtttac cttctattat atttgaaagt gattttaaag gtctgttatt aggccctaca
     3961 actgttcttc cacgtcttcc gttatcaatt aaagcgtcaa ctgcttcttg aagcatcctt
     4021 ttttcgtttc ttacgataat ctcaggtgct cccatttcaa gaagccttgc aagacggttg
     4081 tttctgttta ttacacgtct gtataaatcg tttaagtcag atgttgcaaa tcttccgccg
     4141 tctaactgta ccattggtct taaatcaggc ggtgttacag gaagaacatc cattaccatc
     4201 catgtaggat ctgtatttga agaaatcaaa gaatccaaaa gtcttaattt cttaatcaat
     4261 ttggcttttc tttgcgcgga agaaatttgt ccttcaagct ctgttctcat ttcttctgcc
     4321 attactttaa tatcaagttc tgaaagaaga tcttttatag cttctgcacc aattcctact
     4381 ttaagctcgg tttcttcgtt ttctattaag taatcttcgt attcttcttc gcttaaaagc
     4441 tgatattttt tgaatgtgct ttcacccggc tctattacaa cataattatt aaaatatata
     4501 acctgttcta aatctttcaa agacatatcc aaaagcagcc ctaaataact cgggatacct
     4561 tttaaaaacc agatatggct gacaggtgct gcaagtttaa tgtgtcccat tctgtgtctt
     4621 ctgactttag aatcggtaac ttcaactccg caacgttcac agataatacc tttatgtctt
     4681 actcttttat actttccgca ataacattcc cagtcttttg aaggtccgaa gattctttcg
     4741 cagaataaac cttctctttc cggtttaagg gtacggtaat taatggtttc aggctttgtt
     4801 acttcgccga aagaccattt catcacccta tcgggtgaag ctatgctgat tcgtatgtaa
     4861 tcaaaataat ctatacttgt tgacaatgtc gttctccttt ttttaagtaa acagtaaaag
     4921 gatttttcta ttcactattt actatttact atttactttt ctataattct ccgaatgttg
     4981 ccggtgtttc aaagaaattg atattcaaca actctgaatc gatgtcggaa agcgttcttt
     5041 tcggagctga ttttctcaaa tcatccatat cgctcatcaa atcgacttct acaccgtctt
     5101 ttttggcaac cgttatatcc aaacctatac tttgcagttc tcttacaaga actttaaatg
     5161 attcggggat tcccggtctt ggtatgttat cgccttttac tattgcttca taagctctgt
     5221 ttcttccgtt gacatcatct gatttaatag tcaacatttc ctgcagagta taagctgcac
     5281 cgtaagcttc aagtgcccaa acttccattt caccgaacct ttgaccgccg aattgtgctt
     5341 taccgcccaa tggctgctga gttacaagtg aataaggacc tgtgcttctt gcgtgaattt
     5401 tatcatcaac aaggtgaaca agttttaaga cataagccaa acctaccgca ataggttgtt
     5461 cgaaagcttc tcctgttctt ccgtcataca gagtaacctt tccgtcttct cttacccagt
     5521 cgcagcctga acttttaata cctttgataa gttcttcttt tgttgcaatt tctgattgat
     5581 tatctccgta catttcatca aacggaggtg cttcgtaatg gttgcctgtc aaataagccg
     5641 ccaaaccaag caaacactcg taagtttgtc cgacattcat acgagaaggt acgccaagtg
     5701 gattcaaaac tatatcgata ggtgttccat caggcaagaa aggcatatcg ttttgaggca
     5761 atattttaga aacaataccc ttgttcccgt gacgtcctga aagtttatcg cctacagaaa
     5821 ctttgcgttt ctgagcaata taaaccctta tgaccgtatt tgctcccggt ggtaattcgt
     5881 cgcctttttc tctgtcaaac actttaacgt caacaactct tccgccttct ccatgaggta
     5941 cacgaagcga attatcttta acatctcttg ctttttcagc aaaaattgct cttaaaagct
     6001 tttcttcggg cggatgttcg gattcacctt ttggtgtaac ttttccgacc aaaatatcat
     6061 cagcgtaaac ccttgcacct attcttacaa tccctctttc atctaaatgt cttaaagact
     6121 cttcagaaac attaggaatt tctcttgtaa tttcttcagg cccgagtttt gtctgtcttg
     6181 catctatttc aagtttttcg atgtgaacag aagtgaaaat atcatcctgt acacaacgct
     6241 ctgaaagcaa aatagaatcc tcaaaattaa aaccttccca gggcatgtat gccacgagaa
     6301 tgtttttacc taaagaaagt tctccgccgc aagttgaagc accgtctgct ataacatctc
     6361 ccgcattaac cttgtcacct tctcttacga ttggtctttg gttaatacaa gtatcctggt
     6421 tgcttcttaa atatttcata agctggtgaa catggagttt gccggcttta tctttaattg
     6481 taatttcttc tcccacaact ctttcaataa ctccgtcaac agttgcaaca gcaaccattc
     6541 cggaatcttt tgccgctctt gcctctaaac ctgttcctac aaccggtctt tgggtgataa
     6601 gaagaggtac ggcttggcgt tgcatgtttg aacccattaa cgcacggttc gcgtcgtcgt
     6661 gttcgataaa cggaatcaaa gaagtcgcaa cagaaataat ctgaatagga gaaaccccca
     6721 tatagtcaac tctttttgat tcgcccattg taaattcaga acggtaacga actggtacat
     6781 attcgtgttt tatacttttg tctttgttta attgcaaatc agaaggcgcg atacggtagt
     6841 tttcttcttc gtcagctgat aaatagttaa cctcatctgt tacaaccccg tcttttacca
     6901 caaaaaatgg tgattcaaca aatccataat cgttaacttt tgcgtgagtc gcaagtgagc
     6961 ctatcaaacc tgcattagga ccttcgggtg tttctaccgg acaaattctt ccataatgag
     7021 aggggtgaat gtcgcgaaca gcaaagcccg ctctttctct cattaatccg cctggtccta
     7081 aagccgaaac ccttcttttg tgagttaatt cagccaatgg gtttgtttgg tccataaact
     7141 gagatagctg cgaacttccg aaaaactctt ttaaagaagc caccaaaggc tttgtattta
     7201 ataaattcaa aggagtcaaa gtttcagaat cctgaagcgt cattctttct ttaacaattc
     7261 tttctatcct tgaaagccca accctaaact ggttttgaag caattcgccc accgagcgga
     7321 ttcttctgtt tcctaaatgg tcgatatcat cgattgttcc ttcatcgtaa gttaaattaa
     7381 tcaaatattc tattgaagcc acaatatcct gaacggttaa ggttctttga gattcatgaa
     7441 cattcaaatt taattttttg tttaatttgt aacggccgac tctgcctata tcgtattttt
     7501 tctcatcaaa aaatcttgct tcaagaattg agcggccacc ctgaggagat gcaggatctc
     7561 cgggtcttaa tttcttgtaa acctccacca aggcatcatc agaagtttca gccgtatctt
     7621 tttccaaagt ttttttcaaa aactctgcgt gagtaaataa tgtttccatc tcgggagcac
     7681 ttattcccat tgctttcaat agcgttgtgg caaggatttt tctgtttttg tcaattttaa
     7741 cgtaaaccaa atcgttagaa tcagtttcga ttttaagcca tgccccgcga ttaggaatca
     7801 aagttgcatt ataaagacgt tttcccgtag gagaaatttc ccttttaaag taaacaccgg
     7861 gagaacgaac gatttgtgaa acaattacac gttccgctcc gtttacgata aacgttcctt
     7921 tgtcggtcat ggtcggaata tcaccaatat aaacttcctg ttctttaatt tccccgctgt
     7981 cacggtttac cagccttacc attacacgaa gctgtttagc gtaagtcgtg tcatggattc
     8041 tagcttcttc tattgtatat tttgcgttat caaatgtata atttggcaaa aaatgcaatt
     8101 ctagtcttcc ggtatagtct ttaataggcg aaaaagaaag taattcttct tttaagcctt
     8161 cttttaaaaa ccattcaaaa gatttttttt gcacttcaat caggtcgggt aagtcatcca
     8221 atctggtatt gggaataccc attggtgtta ctcgttttgc atattgtgtt ttagacatta
     8281 attcacctcg tctgtgtttg tggtgtacgt acttattaaa attttcaaaa atattcggct
     8341 aaagcatgcc ctattttaca aaagcatgtt atacaatcgt acaacttcaa tgatgttagt
     8401 caaggaaaaa agtcatttaa aaatatttgt gttcacaatt ctttacaatt agggtttttt
     8461 aaggggtttt tagcgcaaaa ttattaattt tattggtttt ggctgtcaag tggggcactg
     8521 taaaatagta aatagtaaat ggtaaataga aatgttaaaa caggggacaa gaggcgaaga
     8581 taccaagagg cgtagtagga taacccacgc atccaagcat ctaaccatct tatcttctta
     8641 taaactcttc aactgttcga cttttcaact accctatcgc ccattttccg caggttccgc
     8701 cccagcgggc tattatattc ccttgaatat cttttatttt ttctacatga agaacctcac
     8761 actcaccttc gacaatggtt atgacttcac agttatttgc gcaatcatga caatcgcagg
     8821 taattgaatt aaagtgcatt tctcccacct gccaaccttt aaattttgtt tcattacctg
     8881 tatattgatg attttccata gctaaaagtg ccgcccctat tgcgcccatg gtttggtgat
     8941 tattgggaac aacaactttt gtgtttaatg cctcttcaaa cgcttttacc atgcctgaat
     9001 ttgtcgcaac accgccttga aaagtcaccg tgggtaaaag ttctttaccc agtgccaagt
     9061 tgctcaaata atttcgcacc aatgcctgac aaagcccgta taacaaatct tcgacagggt
     9121 atccgagttg ttgtttatga atcaaatcgc tttctgcaaa aaccccgcaa cgtcctgcta
     9181 ttcttgcagg atttgttgat tttaaggcgg tttcaccaaa aagctctata gggacattta
     9241 atctgtttgc ctgctggtcg agaaagcttc ctgttcctgc agcacaaacc gtattcatgg
     9301 caaaatcggt cacaattccg tctcttaaaa ttataatttt gctgtcctgt ccgccgattt
     9361 caagtattgt ttggactccg ggcacattaa tgcttgccgc aacagcatgt gcggttatct
     9421 cgttttttat aacatctgca ccgaccagtg cccctgcaag atttcttccg ctgcctgttg
     9481 tgccgacgcc tttaacgtca tattcgcaaa gtgctttttt atataattct ctaagccctt
     9541 cttgaaccgc caagacaggt tttcctgaag ttcttaacat atagctgtca ataaatttcc
     9601 ctttttcatc aacaagtgcg acttttgttg tcaccgagcc tacatctatt cctaagtatg
     9661 ttgttaaacc cattatcaat ccttttttta gtgaatagta aatggtaaat agtaaataaa
     9721 aatttcaatc tattcactat ttcttatttc ctatttactt tttcatcttc tcaaaaacaa
     9781 tttttttaaa caagcgtttg tataacatta atcacaaaat caagtgcacc ttgattttgt
     9841 tcaaaaactt ttttgcaatc ctggcttgcc ttcttataat agtcttcatc cgtaaataat
     9901 ttttttaatt cattttttaa ctcttgtttg ttttggacaa cttttcctgc cgaagtggaa
     9961 gttaaaatag tataaatatc tttaaagttg cggatattcg agccagaaat aacaggtttt
    10021 tcaaaaatta ttgcctcaag ggggttatga ccgcctgttt tgttaaaact tccacccaaa
    10081 aatgcaacgt cacaaatcga ataaaattta gacaactctc ccattgtgtc aattaaaagc
    10141 atgtcctcat cgctaaattt ccactccaaa gaccgtcttg cggtttttcc ttttttctgc
    10201 attaaattaa gtacgctctc aattcgctca ggatggcgag gtgctattat caattttaag
    10261 ccggaaattt ctttttttaa atcaccgaaa acatcgagta taatctcgtc ttctccgcta
    10321 tgtgtgctac cggctaaaag tattttgtta ttcggatttt tattaaaatt caagtctttt
    10381 gtatcgggtt ttttaatgtc aaattttaaa ttacccataa cttttgtttt atcagggttt
    10441 gcacctattg aaataagttt ttccatatcc ttttggcttt gcgggtaaat tccggtatat
    10501 ttgtgcaaaa taggtgcaaa aaaccatgac aattttttat aacttttata agtcccgtca
    10561 gatatccttg cgttaattat aaaaagtggg atttttttct tgtttaaaat atatgcaaaa
    10621 ttaggccaca attcggtttc catgataaaa acggcttcgg gatttatctt gtttaaaaaa
    10681 ttgtttatgc aaaaaggaaa gtcatatgga aaataagaaa taaaactaca gacttcgctt
    10741 aatttttttt gtgcaatttc ttgtcctgtt tgggttcccg tcattaaaac tatgtttttt
    10801 tgtgggaatt tttctttcgt ttttctaatc aaattttcca tagctaaaac ttcaccgaca
    10861 gaaataccgt agaatactat aggtgaagag tgaagggtga agggtgaaga ataaaatcca
    10921 catttttgtt taaacccgac aagcagtttc ggcttaaaca cccctaaaac cagccaaaaa
    10981 ggaagtatta aaacaaatgc tattgtagat aaaattccat aaataaaaaa catatcaaac
    11041 attctctatt ccgtcatcct gaaattgttt caggatctat ccaacaaaca acaggtggta
    11101 agatgctgaa ataaattcag catgacatat attattatat tcaaaacatt actttttgtc
    11161 agagaaaata aatttttgtt tcattattga gtgaaaactg attttatgcg aaaataataa
    11221 aataaaccga tatttaagtt gttaatttag aaaggcgcgt aaacaatatg tttcgtgtgg
    11281 tgacaaaatg caagacaaaa cttttttaat gattccagga cctacgcccg ttcccgaaag
    11341 tgtacttttg gctatggcaa aacacccgat aggacacaga agtgcagagt tctcggaaat
    11401 tttaaaaagt gtttataaag atttaaaatg gctttttcaa accaaaaatg atgtctttat
    11461 ttatgcggca agcggaacag gtgcaatgga agcagctctt tcaaaccttg taaatcaggg
    11521 cgataagatt ttatcgttaa ttatagggaa ttttggcgca cgctgggcaa aaatagcaca
    11581 atcaagaggt gctgttgttg aaaaattaga agttgaattg ggacaagtaa taaaccccca
    11641 agatttaaga acaaaacttg cccaagacac aatgaaagaa attaaaatcg taacattgac
    11701 tcactccgag acctcaacag gtgcaaaaaa cgacatcaaa gtgctttgcg aaataataag
    11761 aaaacacggc gcaatctctg ttgttgacgg ggttacaagc gtgggtgcga tgaatgttaa
    11821 aatggatgag tggggaattg atgttcttgt atcaggctct caaaaagggt ttatgatacc
    11881 tcccggactt gcatttcttg ccgcaaatga aagagcatgg caagtacatg aaaaatgtct
    11941 ttatccaagt ttttactttg actggaaagc tcataaaaac gctcttacgg atgacacaac
    12001 accatggacc ccacccataa accttatttt tgcgcttcat gaagccttta aaataatgaa
    12061 aaacgaaggc ttagaaaata tttacacaag acaccaaaat cttgcaatgg ggctcagata
    12121 cgccgttagg gccttgggat tagaattatt tgtaaaagaa gacaaaaacg caagttatgc
    12181 cataacatcc atttacccac cgaaaggaat ttctgttccc gatataagaa aagtttttaa
    12241 aaatgactat gatatcgtgg ttgcaaacgg acaaaaccag cttaaagaca agattttcag
    12301 aatgggaact ttaggttttg tttccgagcg cgatttattg accgcagtaa gctccttaga
    12361 ggccgtactt caaaaactcg gtcacaaatt tgaaaaaggc gcagggttaa aggctgtttt
    12421 agaatattta aaataattgt ttatattgag gcaagccaaa ttggtcatat ataaaagctg
    12481 aatgattatt ttcatttaaa atacaaatct cttttttgtt tgaagtattt tcaaaccaac
    12541 tgagatgttt gtcctctttt tctctacaac cctgaacttc tgcagtataa acataaatta
    12601 aactttgtga tggttttatt tttgttttaa ttattttaac attttctttt gaaattttat
    12661 cataatatga cacttcttca taaaaagggg ctttattatt gtgctcaaaa taaaatactg
    12721 ctttttcttg cgttttaaaa tcttctattt gtattttttt aatttttcct gtcatataat
    12781 attcatattt ttgctcacct gaaggtgttc ttatttctat tgttttgggg taataagggt
    12841 tatcccaata ttcaataaat ttgtttgtat taatttcata atttttttta ggattcattt
    12901 cattataaaa gttttgatct gccatatctc tttgattttc ggcatcttga atataataat
    12961 caagcctttt taactctttt tctaaattta ttgaagaatc tttgtatctt gtatataaat
    13021 cttgtgattc aacccagtct tgcttcaaaa aatcccttga ttcaatatat ttttgctgag
    13081 taaaactaac agagccctga tgattatttt ttaaactatt agttttatta ctgtaaagga
    13141 tattattttt ttgaaaagat tttattgcag atacttgcat tggtttctcc ttttgatgat
    13201 tttaaaacct atattaaaaa ttttttgctg ttttattaaa tattctttac aattctatca
    13261 gcaagtggag ttaaaacaat tccattcaat gttttgtcga tttttttgag tttttttgca
    13321 actctttcca tttcatctgt ccatacaaaa tgttttaaaa cataattttc accctttttg
    13381 aaatctccgt caagttgtat tcttataatg tcttttaaca ttttttgcgc agtcggaatc
    13441 attttttcca aatcaatatg taaaatacct ttatcatcga cttttattgc cttattttca
    13501 agaaaatatt ttgtctgcat gacagagcga accctatgtg cctgacttaa agtcggtttt
    13561 gcctttagga aattatccgc cataaaagaa accaaaatct gttttttctg aaattcgctg
    13621 tacataccct ctctagtcaa aatatcaagc attgctaaag atgccatatc ggctttgttt
    13681 tcttctatta tgtttttgta tttaccaagt gcctctgttc cttctttggg gcccaaagag
    13741 tgtgcgtttt catgcccgat tgtaaaccag tgatctgctt ctttgtcata aaatttatgt
    13801 aattttttat ccaaaatggc atccaatctt ttttgaagct ttttgtaatc ggaaatttgt
    13861 cttacctgcc ggtgataaac atttcttctg ccgccgccta tggttaaaga cagtttgtcg
    13921 ttatttggca gattttgtgc caaggtcata ccacctctaa aggctctttc gtcgccagaa
    13981 agtgttacta tatcaacatc caccatggtt tgggggtaaa tgcattcttg tttttttgtt
    14041 tgttttattt tttgattata ttccttgtaa aacggcatgt tttttgccag aagcggaata
    14101 tatttttcta tctcaagcaa ggtttttgtg ccttttttgt ttactattcc aacccttgtc
    14161 ccgattgaat cttttgaaag aggttcgatt ttgtgttttt tcaaaagtgc ttttaattcc
    14221 ttgttttcaa ccacagagcc tgtcatttca tctgaatact gttctcttga aatagtaaat
    14281 tcaagcgggg tgttttgaag gctcgcccat tttttatctg cttgcgcgtc aagcatcgga
    14341 ttattttctt taagtgcttt tgcctgtaaa attaaatatt cattgaaaac cttattagta
    14401 gaatattttg ccgcctcttc caattcttgt gctgcttctt taaattcttt ttcaaaagca
    14461 tttgtgtaat caagagcttt tagctctttt ttatccctta caatcataga acgttgatta
    14521 agaatttctt tgacctcttt tattttgcaa tctttgagca tttgtattaa aatttcgtga
    14581 aattcattta cccctaaatc attagggtaa aaacctttgc ccgctgattc tttttctcct
    14641 tttgctaaga taattttatt ggactcagtg tcaaccgcaa taatacctct ttgtgcttga
    14701 aaaagcttga atgccatttt tgctttttta tttccctttt ttatttcttc ttttaaaaac
    14761 tctctaaatt caatattgtt tgggttatct tgttttaaat aaacttcatc aagaataatt
    14821 gctgctttaa ttaaatgctt tagtgctttt ttatcaccaa tttttaattt tttatactct
    14881 ttgctgtctt ttttgagcat cttttttgga acaagatagg ttttgcttgt tctttttttt
    14941 aattcttttt cgtctaacgc tatttcaaaa gtttttttca ttacccctcc cctttttgtt
    15001 taatattatc atttttatac tcttgtgtct aatgcgtttg taatattttt tggtataatt
    15061 ttaaaaaagg aggtttatat gctgctttta tatgttcttg aaggatgccc ttactgtaaa
    15121 aaggtaataa ctcatttaga agaaaaaaac atagaacacc gcaggcttga tgttaacgac
    15181 cctgttaata tggacgaact tttgcatctt ggcagagagg atcaggttcc ttttttggtg
    15241 gacaccgaac acaatgtaaa aatgtatgaa tctgacgaga ttattcaata tattgatact
    15301 ttataaaatt tttccttctt ccaccttata aatttttaca tcttttaaaa attttttgtc
    15361 aaataaaagc gtgtcaacac ttgttattat tgtttgagtg tttttatcta tggttttgag
    15421 caaataattt tgtcttaaat catccagttc ggcaagaaca tcgtccagta ataaaacggg
    15481 tttttctcct gttttttgct ctataatttc tatttcggca agttttaagg ataaaactat
    15541 tgttctttgc tgtccttgag atgcaaattt tattgcttct ttattgttta taaaaaacga
    15601 aacgtcatct ctgtgggggc ccacaagagc ttgtcctctt tggatttctt ctgtttttct
    15661 ttcttgaagt ttttgcataa atttttgaga aatttcttct atctgttctt cctcggcaag
    15721 aaacgggcag gtataaaaaa ttgttagttt ttcatctttg cttatttttt tatatttttc
    15781 ttgagaaagt ttttcaaaat cttttaaaaa cttttttctc aaaaatataa tattgcttcc
    15841 taaaatacaa agttgttcat tataaacatc taacagggtt tggtttccgt tataatcttt
    15901 taaaaaattg tttttttgta ttcttatttt gttgtattta gaaagtctct cgtcataggc
    15961 cggataaatt tttgaaataa cctcgtcaag ccaatctctt ctatcttgag gggagcccct
    16021 caaaagaagt aaatcttgtg ttgtaaaaag aactgctgaa agaatatttt tgaagttttt
    16081 tggcgttgtt tttaatccgt ttatttttaa ttctcttttt ttttctttat ataaatagtt
    16141 tattcctgtt tcaactcctg ttttttttat ttttgttttt atttcaaaat tatcttctcc
    16201 gaaagtcggt atttcttgaa tgtttgatgt tcttgggctt ttaagtgttg ataaaaaata
    16261 aatactctct aaaatatttg tttttccttg tgcgtttttt cctacaaaaa gtattttaga
    16321 aaaatcaagg tttatttgtt ttttttccaa atttctatag ttttttaatt ctgtttctaa
    16381 aatcttcatt tttttattat atcaaactaa taataaatgt agaaaaatgt aaattgtttt
    16441 tataaaggtg tcttttttaa tttttattat ctttttttat aattttctac atttttttct
    16501 acatttattt ttgagttatt gcttttttta atgttctttt ttatattttt ttgttgctta
    16561 ttattaatat tatattttaa taaattattt atataatata atattaaata aagaaaaatt
    16621 ttttgtagaa aacttttaaa gaggtgtaaa tggaatttgt agttaaaaaa gaagatttat
    16681 ttaatggaat aaaaattgtt gaaagagcaa cagcaatgaa agggcttcaa cctgttcttg
    16741 caaatatatt aatagaaaca ataaacaata aaactttaaa attaagcgcg acagatttgg
    16801 atttgactgt tataacaaca attcaggctc aaattgaaaa agaaggaaaa ataacacttc
    16861 cttcaaaaac attaagtgaa attgtaacaa ggctttcaga aggtttaata agtttttcaa
    16921 aaacaacaga tacaaatact gtagaaataa aaagtaaaaa ttctaaattt gaaataatag
    16981 gaattagtgc aaatgaattt ccttctatac ctgaaattga aataaaagaa gaaaattcag
    17041 tcgaaattga aataaaacct tttacaaaag cgataaaaca agcagggttt tccgcagctg
    17101 gatacgaaac gaacaatttg ttgtcgggag ttgtttgcga tatttctcaa aacactttag
    17161 agatggcatc aactgacgga aacagacttt gtcgggtaag agaaaaaata gaaaattcgg
    17221 gaaaaacaag caatcttata attccttcaa aaacactaca ggaatttatt aaaatgagtt
    17281 cttttattga agaagataaa ataaaaattt atacagataa aacaaaatta ataataaaaa
    17341 gcgaagacat aactattatt tcaagaatta tggaagggca gtatcctaaa tataaccaac
    17401 ttattccaca agaaagccca aaagaagcta ttattaaaat aggtcaacta atagcagcaa
    17461 tagaaagagt tgctattatg gttaatgaaa aaacaagtat tataaaaatg gaattttcat
    17521 caaataaact tgttttaaag gcagatacac ctgatgcagg agcatccgaa gaagaaatag
    17581 atatagatta taaatttgaa gaactaaata tagcttttaa ttatagatat ttgttggaat
    17641 gtttaaaaaa tatggaaaca gaagaggtta aaataggact aaattcaagt ctttccgcta
    17701 ctgttttaaa accaaacaac gaagatgatt atatttgttt aataatgcct gttcaaatca
    17761 ggggttaaag gaaattattg tttgataaat aatttaaaga acgctttgca agaagctcgt
    17821 ttttgagctt tttgtttttt ctttcttttt tatcaatttc aatcggtgct acaattcccc
    17881 aattagaatt tacgggctga aaagacttgt gactttcaaa agaaatatat ttacacaaag
    17941 agcctaacat tgtttcttga ggaagaatta aaagaggctc cttttttaaa taattggcca
    18001 tgtttattcc cgccaaaaga cctgttgcca tagattctgt gtaaccctcg gttcctgtta
    18061 tctgtcctgc aaaaaataaa tcttttctgc tttttgtctg caaagagggg aacaatattt
    18121 ttggagaatt aataaaagta tttttgtgca taaccccaaa gcggataata tttgcatttt
    18181 ctaagcctgg aattaatttt ataagttctt tttgttcttt ccattttaaa tttgtctgaa
    18241 aaccgaccat gttataaagg gtttttgcca tgttgtcttg tctcagttgg acaacggccc
    18301 agttttcaat acctgttttt ttatcaacaa ggccaaccgg tttcatcggg ccaaaacgta
    18361 aggtgtcaac ccctcgggag gcaaggactt ctataggcaa gcatgattca aaaaactttg
    18421 catccttttc aaaatctttt aattcatttt tttcagaaga aatgagcaca ttataaaagg
    18481 tttcgtattc ttttttattc atggggcagt ttatataagt cgcctcgcct ttatcataac
    18541 gatttgccca aaaagctttt tcaaaattta tggagtcttt ttctataata ggagctattg
    18601 catcaaagaa aaaaaagttt tcacttttag taaattcttt aatgttttga gcaaacaaat
    18661 tacttgtaag aggaccggtt gcgacaacag tgggggtgtt tggaattgat attatttctt
    18721 ttcttattgt tttaatgttt ggattatttt ctatagcttc agtaactttt ttagaaaaca
    18781 tttctctgtc aactgcaagt gctccgcctg cgggaacttg acaaagatag gcaactttta
    18841 ttagttcgcc ccccaaaatt tccatttctt tttttaacaa cccggaagcg tttgtaacat
    18901 caaaagaacc taaactgttt gagcatacaa attctgcaaa gtcgcaggat ttatgagcac
    18961 cggttgtttt ttcggggcgc atttcgtaaa gattaacttt tattcctctt ttggcaagct
    19021 gaagggcaca ttctgaacca gccaaacctg ctcctataac atttgctttc atataattta
    19081 acatatataa aaaaaacaaa aatgtcgatg gggggacttg aacccccaca gattgctcca
    19141 cacgcccctc aaacgtgcgc gtctaccatt ccgccacatc gacattcaaa tttaattttc
    19201 aaagttttta tttgggctgt ggcggagtta aaccttcggt ctaacctcct ccccttttat
    19261 aactaaatgt tataaaaaag cggagtcctt accacatcga caaaaaatta tttaaaaaaa
    19321 ttggcaagtg ttatatctat tatattataa aaaacagacc cacaaaaaca aaaaagggtt
    19381 ttgtaaacta atgttaaaaa aaataataaa aaattaaata tttttgccaa ccctgccgaa
    19441 gacatgaaag ttagcagtaa aaatgagggt tgtgtgcgat gagaaattat gattatgaaa
    19501 gctttaaaaa tgctttaggc aagatgaaag aagacatgta tcttgaactt caagatgaca
    19561 tagaggcagt taaaaaaaat gtagaaaatc tgttagaaaa agtttttgaa agcagtactt
    19621 ttaaggctga aacaagacac ccgggtgtaa gcgggttcta attttgtaaa aaaagagtta
    19681 taataacaaa caaaaaggag tttataatgg tggacgaagt attttttcat gctctgcaaa
    19741 aaaatattaa taaaaaaatt aaaaaacaaa cgaaagcaga aaaagaaaat ttaaaaaacg
    19801 aactctctgt ttttcaggaa aagaaagtgg tttttaataa tgattttcca tttttacaaa
    19861 gaattgaagt aacaaacaaa aacggaaaag aaacatataa acactatgag cttaaggacg
    19921 gattaaaacc cgaagaatta atcaaagatc caaattggag aaacaatatg gattattggc
    19981 aagaagtgga tgttaattaa tttttttaaa aaataaaaag aaaggagaat aaaaatggta
    20041 gaaaatgttg acaacaacgc attatttggt cgtcttgtag taaaaagcgg cgatggaata
    20101 gcacaaacta ttttaaaggc ggcaaaagca aacccttcaa tttttgaaat atccgaaggc
    20161 accgttaatg ccgaagaaaa aagaagggta aaagaagttt taaaagaaat tcaagcagag
    20221 cgaagaagag acggacgccc ggatatttgt cgtagaaaca atattgatct tgtttacaag
    20281 ggagatgttt tagaatttac ccccgaagaa caaaaaagga ttatggaagc catggggctt
    20341 ggtttgaaaa aacaagacaa aatttcactg gaagatgcca gagaattttt tgggcttagt
    20401 ggtcaagaag gcgaaggcgg gtttcaaaaa tcgaaacaaa cagagttaaa tattcctttt
    20461 gaaaataccc ataaacctta ccaacaatct tatgtggagg cgttaccgcc ggaagaaggt
    20521 caaacacttc cacctccaaa cgatacaatt aatacagaaa acaacaatgt tgaaaaccct
    20581 tttaccccgg ccaatcaggc accagaagaa gatttagaca ttgaaataaa aattgatgag
    20641 ccgcaaataa atgatactct tcctgtttca ccaattgaaa ttgaaaaagt agaacaaaag
    20701 attgttaata tagacacacc gcttcaaaaa acttccggaa taaatacaac taaccccaat
    20761 gtttcagaag ttaataaaac accccgctct cctgagtttg atgaaaaaat tggtcaaata
    20821 accggcaagg tacaagagtt aacagggctt tattccagaa tagcaaaatt aactcccgat
    20881 ataaaaaatc ttgcgagcga acttgatcaa atggatgcct ctcccgaaca aaaagaagag
    20941 ctaaaaatac ttttaagcgg tgtttcagag gcactggggg aaacaaaata aaaagtattt
    21001 atctctttaa aacgacaaaa agaaggtttt aaccttcttt tttgtcgttt tgatttaaac
    21061 atgatataat aattttaaaa aagaaaggtt taaaatgtct gtcaaaaaag ttttaagata
    21121 tggtgaaaaa tcactcagag agccctcaaa agaggttcat aaagtttcaa gaaaaattca
    21181 ggatttagtt caggatttat tagacacgct ttattctcaa aacggcgtgg ggcttgcagc
    21241 tcctcaaata ggagaaaatc acagagtgtt tgtaatagat acatcatcag gcgatgaacc
    21301 tcttaatccc atggttttta ttaatccaaa aattattaaa aaatcaggag cgtttgtaag
    21361 tcatgaaggt tgtttaagct ttcctgatat gtacacaaac gtaaggcgtt acaaaaatgt
    21421 tatgataaaa gcaatagatg aaaaaggtcg ctcttttgtt cttgaggcaa aagacggaag
    21481 cctgcttgct aaagccattc aacacgaata tgaccatctt gacgggattt tgtttataga
    21541 ccatgcaata aatagatttg aggcagagca ggagcttaca caaaaacatc ttccgccgct
    21601 tgaggttgaa aagcttttgg aagaaccgga gctggatgaa gaattaaaaa aagaggcaaa
    21661 acaagcaaaa taatgataaa agtaatcttt tttgcgacac ctaaaatagc gttaaaatca
    21721 cttgattatc ttgtaaaatc cgataagata gaggttttag ctgttgtaac ccagcctgat
    21781 aaaccctcag gcagggggca taaaatttta ccttcttcaa ttaaagaata tgcactggag
    21841 cacaaaatac ctgtttttca gccacattca ataaaaaaag aaatacaaat tcaggaagca
    21901 ttaaaaaaat tatcccccga tttttttgta acttttgcgt tcgggcagat tttatcaaaa
    21961 gaagttttac agataccaaa atatgtcaca ataaaccttc atgcatcact cttgccttta
    22021 tacaggggcg caaatcccat tcagtgggca cttataaacg gggataaaaa aacaggtatt
    22081 tgtactatga taacagaaga agggcttgac tgcggagcga tttgtttaaa agacgagata
    22141 aatataactc caaatatgaa ttatgtagaa ctttttgaat taatctcgca aaaatcgccc
    22201 gaacttattg aaacaacgct tttaaatatt aaatcagggg cacttaagcc aaccgcacag
    22261 tgctgcgaaa atgtgactta tgctcataaa attaaaaaag aagattcaaa aatagattgg
    22321 tcaaaatccg ccttgcaaat tcataatttc gtaagaggct tatatcaatt tccaagcgca
    22381 tatacaacat ttaagggtaa aaacataaaa ataatggaaa caagggtaaa tgagggggta
    22441 aataacacaa ataaaacaca gaatggtgaa atttttgacg tatgcaaaga tggaataaaa
    22501 gttgtaacaa cagacggagt gcttataata aaaaggttaa aacctgaggg aaaatccgaa
    22561 atgagctcta aagactgggc aaacgggatt aaaaatttaa tcggagaaaa gtttgaatga
    22621 ttacaaaagg cataagaggc gcaataacgg ttgaggaaaa cacaaaagaa gctatagaag
    22681 cagctgtggt cgagcttatt gagcaaatat tttttaaaaa caatattgaa aacgatttaa
    22741 tttctcacat aatttttaca acaacaaagg atttaaccgc agagtttccg gcaaaaattg
    22801 caagagagca ctttgattta aaaaatacgg caatgatttg tgcgcaagaa ataaatgttg
    22861 aaaattcatt aaaaatgtgt cttagatttt taatagttgt taactgttta gagaactttt
    22921 gtccaaaaca tgtttactta aaaggcgcaa aaaatttacg ccctgatatt aaaatgtaac
    22981 aaataaaaat aattttgatt ttaaaaaacc agcatatata ataatcttat gaaaagattt
    23041 ttaacgattt tgggactggt tttaatagta atttttattc aggttttgtt caaacctttt
    23101 tttaacaaac ttgaatggat gacttatgac ttgagagcaa ggttttcaac agacaaaggc
    23161 ccttttaaca aaaaatttaa caaagcggat aaaaaaattg ttttaatctc tattgacgat
    23221 tattcaagaa aacaaatagc aaatcatcct gaattgaatc tcggcgcatg gccctggaga
    23281 agaaatgttt ggggtgaggt tttaaactat atagagcaag gacacccgag ggcggttttg
    23341 cttgatattg tttttaatga tatttcaaga ggttatgatt atgacaatca atttgcgcaa
    23401 gagttaaaaa agtataataa tgtagtgctt gcaacttcat taaataatcc taaatacctt
    23461 tccgacaaaa taaaaagcga cgatgttgta aacagctttt ttcttccgac aaaaaaaccg
    23521 cttgatgttg aaaattttga caaacaaata gattacaata taacctattt gtcacatgct
    23581 cctatttata atatttacac ggaacacaac acaatggggg ttgttaataa agttgtgggg
    23641 accgattcaa ttataagaaa agtccaacca atttttaagc tggaaaaaaa taagcaggtt
    23701 tattacattc cctccctggc ttttgccgga tttcttaaat atatgggaga gggcggtaaa
    23761 attacaataa aacataacaa aatattttat aaaaacaggg ttataccaat tgacgacgag
    23821 ggaacgaccc ttttaagctg gcatggaaaa ggaaatgatt acacatatat tcctatttca
    23881 aaaatatttt taagagaaaa aataaacggc aaaaaaataa ccccaaactt ttttaaagac
    23941 aaaatcgtca taataggaag aacagaagca ggaacagata ttcatgcaag tgctgtaaac
    24001 ccaacatacg caggccccga agctaatgct gcagggatag ataattttat caacgataca
    24061 gatgaaaaaa gtaatttaaa aaggaaattt atttcacaac ttcctccttt tataaaatat
    24121 gaaataataa tctttttctg tttaataata gcgtttatag ggctaacttc aaaaaatgca
    24181 atatatgctc tttttaacag tttattcctg gtgtttttgt atttatttat ctcgttttat
    24241 gcttttgcgc atcctggtat aaggctttgg atacctataa ttgagcctct tttttacata
    24301 ttactcacaa ccggtataat tttggcacta agattacaca aagaaacgac acaaaaaaga
    24361 gaaattatga atatgttcgg caaatttgtt tcaccgaatg ttttgtcaac cattattaaa
    24421 aatccttcaa aattggttct taaaaattca aaaaaacgtg taacaatatt attttgtgac
    24481 gtaaaagatt ttacaagtct ttcagaaaaa tacgacccgc aaaaattggt tgataatctt
    24541 aatgaattat ttaatgagat tgtaaatata atatttaaaa ataacggaac ggtagataaa
    24601 tttatcggag attcggttat ggcttactgg ggtgatccca tagcctcacc tgacgatgcc
    24661 tacatggcgg ttaaaacagc tcttgaaata aagaaaaaag taaatgaaat gaaattagaa
    24721 aatgccaaaa acaataaaat aatctttgat gtaaaaatag gtattaacac aggtgatgcg
    24781 cttttggggt tatcgggttc aaataaaatt atgagttata ccgcaatggg agatgccgta
    24841 aataccgcat caaggcttga atcggcttgt tccaagctca aaaaagatat tttaatatcc
    24901 aaatcaactt acgaaaaagt taaagaaaaa attctcgcca tttatgttga caaaatcgag
    24961 gtaaaaggca aagacgaaca aatagaggtt tatgaacccg ttagttttgt tcaggaaagt
    25021 ttaaacgagg tttaatatga gtgaaaaaaa gattaaaaaa tgtcctttta attccggtga
    25081 ttgtactaaa gaatgttctc ttttcataga tacagatgat ttaaatgaac ttcttgctgc
    25141 aaggcttgca agtctgggta ttttagacaa ggaaaacgga atttgtgcta ttaaaacaat
    25201 agcaatgagc acaggccgct ttatttttga aaatacctca accaaaagat tttaaaaaat
    25261 ttattaacaa atgtaacaaa aaatatgctt cttgaaatcg gactataaaa atatttttta
    25321 tatatatgta acacgaagat attttaagga gctttataat ggattttaca ttcagatttt
    25381 cacctgaaag ttcaaaaggt caagaagcat ggagtaattt tttcttgtca tctgatagtg
    25441 aagaaactca agcagcaggt gctattgcac ttttggatgt taatgattgt caaggagata
    25501 ctttttgttc taaagatgct tttggaacaa cagatttttc aaacatagga gaatttgttt
    25561 gtagcgcaga taatacttct gtagaaggtc ttggttcaat tgcctttaca ggatttgatt
    25621 caggcattga ttgtgcttca tcggcaggtt cttttgattg cgcatcttct gattgcggag
    25681 cctcttcaag tagttttata ggataataag atataaaaag gcgggctgtg tttttaaaca
    25741 cagcccgcct ttttatagtt tttataaaac aaaattaatc ttttttaaag cagtctctgc
    25801 aaaaaatttc ttttccttca attggtctga aggggacttg tgtgttaacg ccacattttg
    25861 agcagactgc atcatagagt tttcttttgt gtttttgttt gcgtgccttt ctgcattcgg
    25921 tacatctttt tggtttgttt gtgagtcctt tttctgcgta aaattcttgt tcgccgactg
    25981 tgaaaatgaa ttcacaaccg cactcttcac agatgagttt ttcttcttgg tacatttggt
    26041 gttctcctaa tttcagtaac ggtaatgaaa aaatcaagtt ttttcattta atcctttaca
    26101 ttctaatgct tttttgaaaa atttgcaata gagggtacgg aataattcca aaatatacaa
    26161 ttttgagaat tttccgtgcc cgaccttaag aaggtgaaaa ctaagcacag ccttggtaaa
    26221 aggctggcat agtttttacc ggactaaggc ggcttcgtga aaaattgtaa atttttccca
    26281 agccgctaga ggacaccaaa attattaatt tttgtctatt tttaaagaga ttaaatcttt
    26341 aacagaattt ataggaacag caaaacctat tcctatgttt gatttgttgt tatcagggtt
    26401 ataaattgac tggcttattc ctataacttc gccggttgta ttcacaagcg gtccgccaga
    26461 attgccaggg tttattgccg catcggtttg gattttgttt tttgtgtaat ctattcttga
    26521 aacaattccc tgtgttaaag ttcccgaaaa accgaaagga tttcctatgg caaggacttt
    26581 ttgcccgact tttatttctt cggaattccc aagcttgact gttgacaaag gttcttgggg
    26641 ctctattttt aaaagagcaa tatcgttttt ttctcctaga attgataaaa cttttgcttt
    26701 ataaactttg ccggaaatcg tagtgacatc tatatcgttt gagccgtcaa caacatgtga
    26761 gctggttaaa attacgccct gttgggttaa aatacagcct gttccgcttg aaattccttc
    26821 tttaagttgg gcgtcaatac agactatagc gggatttatt ttttcgtaaa cattaatatt
    26881 tatctgttct tcggcatcat aattttgtcc aaaaacagca aaacaaggga aaattcccaa
    26941 aaaaatcata taaataataa attttttaaa taaattcata aaaaccctca ccccttaaaa
    27001 aattttgcat ttttttaaag aaagtttcca cctgcttttt tggtgaacaa aggttaattc
    27061 ttttacttat taaaatttta atgatacaat gtagaaaaat taaaattatt taaggaagga
    27121 gaaatcatgg gtagaatgga tttattcagg cagcatttaa aagaaaaaag agcggtaaaa
    27181 ataatcgcag gtattgacaa ttttgatatc gaaagcatca aaaaagttgt aacagcggca
    27241 aatcaggcag gtgtaagcgc aatagacatt tgtgcagata caaaaataat ttctcaggta
    27301 aaagaaatga caaagcttcc tgtttttgtt tcatcaataa ttcctaagga attagctcag
    27361 gcaatagaat caggtgctga tgcaatagag ttaggaaatt ttgacgcgct ttataaaaac
    27421 ggcacaagaa tgggagtaaa tgaggtttta gatcttgtca gagaaacact ttctttaata
    27481 gataaaacac aaactttttt ttgtgtaaca ataccggggc atgtgagcgt gtcagagcag
    27541 gttgcacttg ccgttaagct tgaatctttg ggaattgacc ttatccaaac agaaggtgca
    27601 gtaatagctc aggcaaaaag cgaaggcgca agagggcttc ttgaagttgc aagtgtttct
    27661 atagcaaata ccattgaaat cacaagaaac gttgatattc ccgttatgac agcatcagga
    27721 atcacttcaa caacagcacc gatggcactt gccgcaggcg caagcgcaat cggagtaggc
    27781 tcttgtatta ataaacttaa ctctcaaata gctatgattg ctcaggctaa aaatttagtc
    27841 gaaagcgtaa attcaagcca aagagaaaaa gttttagtat aaatatttaa aaaaagggcg
    27901 gactgtcatt tgacagtccg cccttttttt aaataaaatt ttatttaccc ctcatttacc
    27961 tctgattttt tctttcaatt cgcaaaggag tttttcaagt tctgaatttg tttttaattc
    28021 ttctttaatt ttttcataag aaaaaagaat agtaggatgt tttttgttta aaaagtctgc
    28081 aatgtttaca aagctcatct gagtcatttc tcttgcaaga taaattgcaa gatgtcttgt
    28141 gtgtgaaatt ttttggtttc ttgcagaact ttttaattca tctagagaga tatcaaagta
    28201 tttggcgaca gtttcacaaa tattttcaat tgtaactttt ttctttttcc cgtcgatttt
    28261 taagaccttt ttaacggtat caacatcaag ctcaatgttt tgaatacttg caaacgcgct
    28321 tactttattg aaggcccctt caagttctct tacgttatcg cagtaattat gggcgataaa
    28381 ttcaagaact tcagatgaaa ttttcattcc tatttcatct gcaagattag ccaagattgc
    28441 tactcttgtt tcaaaatcag gcggtgtgat atcaacaacc aaacccattt ggaatctggt
    28501 tttaagtctt tccggaagtg tggggatgtc ttcaggtagg cggtctgaag ttattacaat
    28561 ttgctttgag ttattgtgaa gactgtcaaa agtgtggaaa acttcttcca tggtgcgttc
    28621 tttcgattcg ataaattgaa tatcgtcaag caaaagcaca tcgatatttc tgtacttttg
    28681 gcgaaatttt gtcattctgt cattaatatc gccgcctttt cttatgttgt tgataagttc
    28741 atttgtaaaa tcttccgttt taatatatct tacttttaat ttcgggtgat taaaaattat
    28801 ataatgacca atagcttgca ttaagtgggt tttaccaagt cctgaggctc cgtagataaa
    28861 aagcggatta tattttttac aaggggtttt tgcaactgta agtgctgcag cttgggcaaa
    28921 tcggttgttt tcgcccacta caaaattatc aaatttgtat tttaaattca aattacaaaa
    28981 agattgcatc tgggcgaggt tttccattgg cgtggcactt tgagtttttt ccgcggtttg
    29041 ttttgctttt tgaatttgtt ttttaagttt ttgagaaagt tcttcatcaa aaattatgtt
    29101 aaattcaatt tctttaccca aaacattttt aaaagcttct ataacctgat tatagtggtt
    29161 ttttcttatt atggaaacag caaaggcttg ccctgtcaaa acagaaaatt ggttgttttc
    29221 atacccgata ggctctaaag gtaaaatcca aggctcaaaa gaagaagcgg gaatttttgg
    29281 ctccagctct tttttgactt cttcccatat ttttttgact tctgaaattt ccatgccctc
    29341 catgctacaa caaaaagagg gaaataggaa ataggtaata tgaaatgggg aaaagaaggc
    29401 aagttataaa cttataaaac aaaataaaaa agcgggcttt tgcccgcttg aaaccaatcc
    29461 ttgaccttat ccctttaagg cattttatct atgcgataaa attacctcca aatctgcttg
    29521 ctccatggaa aaacgattcc tgcaataatt cttgaaaact ttttttcctg atatcttttt
    29581 tgttcaaatc aggagaattt atgaattccg acgtttcttt atagataccc gaaacggtat
    29641 ctgaaagtaa aagtgtagcc atttttcttt tccttgtctc tcttaatatt tctctcttat
    29701 attaataaaa aagaaataag ggaaattatt tactttttat ataataataa tataaaaaat
    29761 ttacaaaaag ttacaaacct tctgtaaaaa attacatgcc gcccaggttt gttgttattt
    29821 tttgcagtgc agggtacata attattataa aaatagccgg catcataaaa ataaccatgg
    29881 ggatagtcat ttttgtgctt gccatctggg ccatttcttc tattttttgt ctttttctga
    29941 ctctcattga atcacaaaaa gcttcaagag attgtacaat actgcttccg agtttttctg
    30001 tttgaattaa tagtgcaaca aaagactgca aatcctggtt ttcatttctc aaaagcaggt
    30061 ttttgtaagc gtcctgtctg cttaaacctg aaagaatatc tttgtttagt ctatcaaatt
    30121 cagttgaaat tgtttttgaa gtcaattcaa attcttccgc aacttttgaa aaagcagagt
    30181 caagccccaa tcctgcctga acacagattg ataataaatc aaccgcatca ggaataaatt
    30241 tcatgaattc ttttcttcta agtgctattt cttttttaat tttaatttcg ggcaatctgt
    30301 aaactattag aataaacaaa atgcatcctg caagaaccat aaaatcaaat tgaatataaa
    30361 gaataaccgc acaaataaga aaagcaataa gggcacctgt tattttttgt gtttcaaatt
    30421 ttattacatc ttcttcagaa gaaggctctc ctgcctgcaa aagaagtttt tttgtttttc
    30481 ctatggtgga ttttgcattt ttttgggata agggttcaag aagttcgtgt aaattacgga
    30541 ttatgtttgc aataaaactt tctctttttt taaattttct tttaaatctt ttttcaagcg
    30601 gagattgcgg ctcatataaa aatatcgccg ttataattgc tgctgctatt ccgattgtta
    30661 atgatattat taaattcatt tttctcctaa aattctattt cgcttgcttt ttttattgaa
    30721 ataaatccta caacaattaa agccatcgcg gttgcaaacg ccatttgtcc gtctttggtt
    30781 tcaaaaaggg gtttcatata ttccgggttc ataaaagata ttatcaataa aacggcaatc
    30841 ggaactatac ctaagataat gccggataat cttgattgag ccgtttgaac acgcagctgt
    30901 ccgatgagtt taaatctttc tctgattgtg gcagaaaggc tgtttaataa ttctgcaaga
    30961 tttcctccga tttctttttg gataagaacg gcggttatga aaaatctcaa atccatactt
    31021 tttggcataa ttcttgtcat acttaaaaaa gcttcttttg tgtcaatacc taaagacaaa
    31081 tcttttttaa cagtttcaaa tacatcggaa atgggtttgg gcatttcttc aacaactgta
    31141 tctatagctg aaaataaagg gtgacctgcc ttaagagaac ttgccataag gttcagtgcg
    31201 tcgggaaatt gtttggaaaa ttcaataaat ctttttgttt ctgtttgttt taagtaaaaa
    31261 gttggtaaaa aaattgctac aattccaaaa agagacatta acttaaaagg ggttaaaata
    31321 aataataaaa aaggtgcagc gcatagtaag cttataaaaa taaatgtgtc tatataaaga
    31381 tttacacccg aaagagcaat taactctttg atcttatttg atattttaaa attttgaagg
    31441 aattcgccaa tctgttgaat tttatattca ctgactttta gcagattgaa ctcttcttca
    31501 tcgtcttcta taatatttgt tttttgttta attcttgtaa gtctttttaa ggtgttaggg
    31561 ctgaggtctt tttctattac aaaaatcgcc cagacaaaaa tagctgcact tattccgata
    31621 attaaactta ggattaaaaa aatcattttt gcaacctttg agtaagagga ttgtttgaaa
    31681 cagtcaagcg cgattttatt gataaatctt tatttatttg tgcctgttgc acctgatttg
    31741 taaaaaggga acttgtgtaa gtgtgttttc tttctttgtt aaagtattcc aaaggaatgt
    31801 ttaaattctt ttctttgagt ttttcaacaa atttaggcct tattcctgtt gagtgatgat
    31861 agcccaaaac tcttgaattt tctattcctg tttgttcaaa aagaaaaata tcctgcattg
    31921 agataacatt tccttccatc cctataagct cagtcacgga agtaattttt cttgagccgt
    31981 cttgaagcct gcttacctga ataattaaat caatagcaga agatatttga gctttaatat
    32041 ttttttcagg caaatcaatt cctgaatata aagacatagt ttctaatctt gacagagcat
    32101 ctcttgggga attggcgtga agggtcgtta atgaaccgtc atgacctgtg ttcatggctt
    32161 ggagcatgtc aagtgtttcg gcaccgcggc actcccctat tataatcctg tcagggcgca
    32221 tccttaatgc gtttataacc aaatctcttg cgtttatttg ccctgtgcct tcaatgtttg
    32281 caggtcttgt ttcaagcctt acgacatgtt cttgttgaag agcaagttct gcagagtctt
    32341 caattgtaat aattctttcg tcattaggaa tttgagaaga caaactgttt aaaagagttg
    32401 ttttacctgc accggttccg ccgcttatta taatgtttaa acgggcttta actgcaagag
    32461 aaagtgtgtc ggagatttct tttgtgagtg cgccccaacc caataaactt tcaaggcttc
    32521 cggcatcttg cttgaatttt cttattgaaa gtgaagggcc gtcaagtgcc aatggcggaa
    32581 taatggcgtt tacccttgag ccgtctgcaa gccttgcatc taccatcggg gatttttcgt
    32641 ctattcttct tcctactttt gagacaattc tttcgattat atttcttaag tgctggttgt
    32701 ctttaaacga aacacttgtt ttgtaaagct ttccgttttt ttcgatatag acactttttg
    32761 gaccattaac aagaatatct gaaacactcg gatcttccaa aagaggatcc aaaggcccgt
    32821 atccttttgc ctcttgtata atttctttta taagacgatc tttttcgctt ttatttagag
    32881 ggacattttt aaaagaagtt aaaagttgac cttctataaa ttgttttata agttcttttt
    32941 gttgttcgtc atcataattt acccatgtgg gtgttgagtt aaccttttca acaagaagtc
    33001 cgtgaagttg ttctttaaga gataaaaaat catttgttcc gtaaccgctt gcagcagaag
    33061 gagtttcgct gcttgatgca ggtacggtta tatttttatt tgctgcgttc aacctatccc
    33121 taagtgacaa cttttactcc ttatttgaat aaacttttaa aatcaaaaaa cggctttctt
    33181 tcaacttttt tggcaaagct gtgtttgtag atattatccg aaagcattgc ggcaagttct
    33241 ttatagcttt gagtaatatt tgcatcagga cccactttgt ttaccggaac tcctttgttg
    33301 atagcagaca ttatagtaaa gtaattgttc ggaattttcc aaaaaacttt ttgttctaga
    33361 acatcttcaa catcttcaac ttttatttcg tcgttttcca tatatctgtt taaaactatt
    33421 ttaatttttt ctttttcata acccaatctt tcaaacaact ccaaacatct ttgacagttt
    33481 cttatagccg gaaggtttac aatggcaacc aataaaatta aatcagaatt atcaagagcg
    33541 gttattgttt ttccgtcaaa actggcactt gtatcaacca caatgtaaga aaaagtttgt
    33601 cttaaaactg aaaataacgt tgaaatctgt tctgcgctaa tgtcttctgc ctgttctaaa
    33661 taaggagggt cggcaagaac ataaaggctt gtgtctttat atttttccaa agtgcttaaa
    33721 agaaaagttt cgtctattcg ggaaagattt tgaattacat aagaaatatc aaatgcaggg
    33781 tttatatcca aaaaagttgt tatatcgcca agctgaagat ttaaatcaat cagggcgact
    33841 ttttcttttg ttatatttgc aatttcaagg gctaaatttg ttgaaagtgc cgtttttcct
    33901 attccacctt tgtttgaaaa aacagttata actctgcagc ggttattttc agggacaagc
    33961 cctgctattt gttcttttaa cttttccagt gcggttttta attcttcttt aataagaggt
    34021 ttgggcaaaa attctcttgc accggctctc attgctttta ttattaagtc tgtagaataa
    34081 tttgaggaag taacaataat cttgcaggtt ttatgattga gagaaatttt atttattata
    34141 tcaagggcaa gttccgtttt gtttgatatg tcaacaatta caagattggg acggttttcg
    34201 agaatataat tgtagccttc gacaacatca acaaaagaat ctaaaatctg aacataatcc
    34261 aattctttta gaaacaattt taaaacatcc gttgattgag tttgtgtgtc tatgattact
    34321 gtttggattt gatttgtcat aggtctatcc tgttgtggtt atctttatcg acttcaacta
    34381 ttttcggtgt tatgaaaatt accagttctg tatctctttt ttcatttttt aaagaagaga
    34441 aaagcaatcc tataccaggt aagtcgccaa tccatgggac ttttgatttg gactgggaag
    34501 atgtgtgaga caaaagccct gctataacaa gcgtttcgcc gtcacctaat tcaactgttg
    34561 tatcaacttt tcttgtctta aatcctggga ttccggattg agatacatat gaactgtcaa
    34621 tttctgaaac ctcaggagat aattttaaca taaccctgtt tgatttttcc agtatagtag
    34681 gagtgaaatt taaaattacg ccggttgtct taaaatcgta agcgatatta ccgtattgcc
    34741 ccatttgaga aggaactgga atttcgtttc ctacgttaaa acttcctgat ttgccgttta
    34801 tggcaagaag tttaggctct gcaaggattt ttactatacc tttttgttct gccattctaa
    34861 gtgcccatgc cactcttgcg gttgaagacg aatagatata ttgcccgccg gtgtttccgt
    34921 taacggttgc attgcttaaa tcctggtggt ttgtaattcc taaaatttgg gttaagaagt
    34981 tgtcgtaatt ttcacctacg gtaaatttag attcaagttc tcttgtaaag ttccttgatg
    35041 cttctgttat tttaacctca agcaaaacct gttttgtagg gctttcggta aggtcaagaa
    35101 gtttaaagtt gtaagctttt acaatgttgt ttatattttc ttttacggtt gtggaagaaa
    35161 tttttcccga taaaattacc ccgtcatctg tgaaggtgta ttgtaaatct tcattaggcg
    35221 caacctcttc tattgcttgc ataaaagcat cggaatcctg ctgcactaca agattaaaga
    35281 aaacaggttt gttgttgtcg ccccaaaata taagacttgt tcttccgcct tttttgccgt
    35341 ttagcataag ttctttcggc gaaagaagca ctaaatcagc taaagaaggg tctgtgattg
    35401 aaattctttt taccggttca tcaaatttta ttaattgggt ttttcctact attgaataaa
    35461 gtttttcgtt tgaatctttg taagggttta aattaactga atctttaact aaatcgcttt
    35521 ttaccgtgcc tagttgttgg ggtatgggcg tgtctttata gtcaggctct gcggctttaa
    35581 ccagttttgc ggttaaaatc gacattaaga acaaaattat taaaagtatg agactttttt
    35641 tcaatttaaa ctcctttaat caaaagttac cttcgttttg acatttgcct ctataacttc
    35701 caccgacggg gcattgctca aaggcgcaat cggctgagga agccccgaaa ctccgcttga
    35761 aggataatca cttatgggta tatttgcagg caaattagga agtgatgagt aagaatcatt
    35821 tgccgaataa gttgaagccg gagatttttt ctttttcaat cctatttttt tatcacccgg
    35881 atttcttgag acaaggatta aatttccttt agagatactc attataagat ctgccatatc
    35941 atctgccggt acttcaaagg ttattgtttt tgcgcttata atatcgcccg ccggaagacc
    36001 tttttcacct tcgagcccga ttatttttac cccttccatt gaccagccgc cttcaaagtc
    36061 acttagaata tcaacatttg agcctgtttt catccggttt gattttccct gaaatttttc
    36121 tgcaggaagg gttaatgctc taaagccttc ttttaattca atttcgtctc cggatatttt
    36181 tgcgatataa gaatcggtaa aaacggttcc ttttattata tcttttaaaa gtactttatt
    36241 tacaacgtca gaacggtttt caaacgcccc gaaagtaggc gtggaaaaag aagaaaaatc
    36301 tatgtcatcg tctgccacaa gctctcctgc tttcaaatct tttgtggcta cagcataaga
    36361 aaaatctttt attgtatctg agccttggag cgtttcaaga attttttctt tttcacccag
    36421 ttgagcttgc atattattaa aagatgaata agaaatcata gtcgccaaaa gagcaacaac
    36481 gattgccaga attatttttg aagtattttt atttgtacgc ataaattctt aacccgaaaa
    36541 attactatgg accccaatcc accctatgag cttgcggaaa aattgtcatt tttcgaattt
    36601 ttccgcatcc tctataacat tttactatac tagtgattta agctcaaatc catactttat
    36661 ttagttgatt tatgtaggat aaaagtcgaa aaatacaaaa ggcataagct ttttgaacaa
    36721 atttttacat taacctttgt taacaatttt tatgggggaa caactgaaag gctgaatggg
    36781 tgaagagtta ttttagttga aaagtcgaac agtcgaatag tttataagaa gataagatga
    36841 gtagatgcgt gggttatcct gctacgcctc ttggcatctt cgcctctttg catctttctt
    36901 aaggcttagc cccacactct gtcatttcta tttatcattt actatttact tttttatcgt
    36961 cgtgttatac ttatttttgt aagaattttg ggaagaatta tgataaaaag agtttttgtt
    37021 gaattaaaag agcacgcacc ttttacgttt ttgggtgctt tgtcgggaat tttgatattt
    37081 gtgttgttaa aaaacattcc ttataaaatt tcttacgggc ttttttatac ttttcatccc
    37141 ttgcatgttt ttttaagtgc ttatgtaacg gcttcgatgt acaaactaca caagaaaaat
    37201 cccgatatga taaaactttt tttgatagga tttttcggct ctgtgggtgt ggctacttta
    37261 agtgattctg ttattcctta tttgggagag attttgttaa agatgccaaa cactgagctt
    37321 catttaggat ttatcgaaaa gtggtatttt gtaaatcctg ttgcgatttt ggggattgtc
    37381 acggcaattt ttgtttcaaa aacaaagttt cctcatatgg ggcatgtttt tgtaagcact
    37441 tgggcgtctt tatttcatat aatgatggca ggagaaaata tgggctcagg aatttctttg
    37501 tatttttgga ttttaatttt attgtttttc tcggtttgga ttccatgttg ttttagtgac
    37561 atagtttttc cgcttctttt tataaaaggt catgatacag attgtccttg cacttgttgc
    37621 ggcaaggatt agaatttaac tcaaaacaaa aacacccaca atggggtgtt tttatttaaa
    37681 atcggagaag cagggatttg aaccctggat acagattact ccatataaca ccttagcagg
    37741 gtgccgcctt aagccactcg gccacttctc cgtgtaattt tataatatca taaaaatatt
    37801 ttaggagtat aattattgta taaattcatt aaaagaggaa attaaaatga cattaaaaat
    37861 cgcagataaa gaatttacat caaggtttat gataggaaca ggaaaatatc ctgattttga
    37921 agttatgcaa aaagcccaca gggcaagcgg tgcacagata attaccgttg ccataaggcg
    37981 ggtggagctt aatgccccgg ggcatgtcgg gttaatggat tatattgatt tggataaata
    38041 ctggattttg ccaaataccg caggctgtgc gacagtagaa gaggctgtta gaatagccca
    38101 tttaagcagg gcaatgggaa ttaataactg gataaaactt gaggtaatcc ctgattctaa
    38161 atatcttctg cctgatccca tagcgactct tgaagcggct aaaattcttg ttcaggaagg
    38221 ctttgtggtg cttccttata taaatgcaga tcctattctt gccaaaaggc ttgaagaaat
    38281 aggctgtgca acggttatgc ctttagcctc ccctataggc tcaggtcagg gcgtaagaaa
    38341 tttagataat ataaaaataa tcattgaaaa taccaatata ccagtaattg tcgatgccgg
    38401 attgggtgtt ccttcagatg cgtcaaccgt tatggaattg ggcgcagatg cctgcctttt
    38461 aaacaccgct gttgcacttg cccaagatcc tgaaaaaatg gcatatgctt ttaaattggg
    38521 aattgaagca ggaagagcct cttacgaggc aggaagaatt ccgattaaag aaatagcaag
    38581 tgcttcatct ccgcttgttg gtgttgtagg aaaataaatt attgttcgct tatttttctg
    38641 atttccgcaa gagccggatc caaaagtctt ctgccgacat tttcaaattg gattgagcaa
    38701 agcgattttg tgccgtagga aattattttt tctactgtgc cttttccgta tttggggtga
    38761 taaaccgttt ccccctgttc aaaaatttgt gctccaagag gattttcgct ttgaatttcg
    38821 gcagaataca caggtaaaat cccgccttca ttttgagtgg aaacatttaa aatttcatct
    38881 tgttcttctt tttcttggct ttcaaagaca ggttgaggag gaatttcttg ttcaagaatt
    38941 tgctgaggag caatttctat aacatcaaga ggacttatgc tttcttgaat aatttcgtct
    39001 tcttcggcag gatttgtaat cgaattttcg ataaaatcca aatcttcatc gcttaaatct
    39061 tcaatggttg taaccacagg ctcttcgggg gtgtttattt cggatttagg tctggtgtag
    39121 atttcatcta cttctttttt aatctgttct tcaactattt cctgttttgc gacttgttcg
    39181 ggtgtaggag gaggcggcgt tacaacaggg ctttcaaaat ttgtctgcgg aaggtcgctt
    39241 aatttaacga taaagggaat gtggttagtg gcacttccat agactataaa ttcgcctgca
    39301 tttaatttat ttaaaaaagt gttgtagcct gcaaaataat tttgaggctc aatcggcgca
    39361 aaaagaaaaa ggtttttggc gatttgtttt aactcgttta aatatttgta agagtagctg
    39421 caaattattg tagtaaaagc gtttttcgtg gtaaaaatct gttttaaaag ttttttatcc
    39481 gaattttcgc ccaaattcac aatcacgtaa acttccttgt caagggtttt tataagagaa
    39541 taagcgtaag aaatcatttc acgctgaatc atctcatcca ttttgctcag gttaaagatt
    39601 gtcgtgacag ggtgttttaa tgaggcttta agctttgcaa aatccttttt ctgctgggca
    39661 aaaactcctt tttcaaaaaa tctcaaaagc ctgtttttaa gcagtaccag ttcaatgtaa
    39721 cctaattgtc ggtactgttc gtcaacaact ttcaaaaagg tttcgaaagg aacaaacata
    39781 tcgggaattg ttttaacata gttttgaagc tcaagaaaaa cttcctgaag cagagctttt
    39841 gtttctgctt tggcatctgt cagccctttt tcgtatataa aattaatggc ttctgaatta
    39901 agcggaagtt taaaactttt tcctgcggta agtctgtttt ttgaaaaatc aaaatttcct
    39961 gcaaaatcaa atactaatac ttttttgccc aaagcacaga gctgggcgtt taaattttcc
    40021 gtcaaaagtc tgctatcgga gattttttcg cagcatataa gcagtttttc tttcaaaaat
    40081 tcataatcga gtttaagcgg ggttttttgc tgggcaagtt cgcccagtaa aatcgggttt
    40141 tgagtgttaa aaagttcaag gatttcctgc ggataaacca ttccaacagg gcttgaaagc
    40201 gaaggtatag caccgttata gcctgttacc gtcccctgat cgtcaaagtt aaaaagcagt
    40261 tttgcaacgg caaagcttcc ttcggaattt gcttcaaggt gcataatttg tgcgataaaa
    40321 gactgaattc tgtcttttat aaccaaaaac cccgacaaaa caagctcttg cgcgcttgca
    40381 tcgtagctga ttttcacata attgtttttg acctcgatta tctgcatgtt ccaacccttt
    40441 tgtagtttat tttactataa aaaatactgt gatttatata tttactccca aattcttaag
    40501 tgatgtaaag attaaatccg atttttatat catttatttg ttttttttac taaaataaaa
    40561 taagatggta gtggattaaa agttagagaa gaaggttttg taaatggtca ctcaagagtt
    40621 ggaaaaaacg gttctttcgc ctcatgaagt aagaaacatt ttaaacgccg ataaccgtga
    40681 aattatcagg ctgtgcaaaa aagccacgat tatccctaag aaaaataaac agggacaaac
    40741 ttacttcacc tcagatgagg ttaagcggct caagaggctt caaaatgaag ttcctgcagt
    40801 tgtgacctca cccattactc aggcagaatc caatcttgtt gtcgcaaatc ttctttcaac
    40861 tttagaaaaa tttgaaaaca atattacaga ttcgctttca aaagttatcg aagaaaaact
    40921 tgaaggcatg gacgaggttg ttgttgagct tatcagatgc aaaacagaaa acgaaagctt
    40981 aagacaaaaa ttaaacgaac ttaacaaaga aaattacaat ttaaaaaaca gcataaaaag
    41041 ctataaatca gtgggactgg gtttttacat aaaaacctct caggatgatt tcagtatttg
    41101 aaagtgaata ggtgaataag taaataagtg aataagtgta attacaatat aatttaaact
    41161 attcaactta tcgactgttc gacttatcaa ctaaaaagga gacgatatgg ttgtaaaaga
    41221 gcaggaaata aaagcgattt ccgaagaaaa aaataaagca cttaaaatgg cgattgaaaa
    41281 aattgaaaag gattttggca agggttctat tatgaagctc ggcgacagac ctatggttaa
    41341 tgtcgaagct attcctacag gcgcgttggc acttgacgtg gcactgggcg tgggtggaat
    41401 cccaagaggc agaattatag aagtttacgg accggaaagt tcgggtaaaa ccactttggc
    41461 attgcacatt gttgcagagt gtcagaaaaa aggcgggata gcagcttatg tcgatgcaga
    41521 gcacgcactt gatcccgagt attcaagaaa tttaggcgta aatgttgatg atttgttaat
    41581 ttcacagcct gatacaggtg agcaggcact tgatattaca gaagaacttg tgcgctccgg
    41641 tgctgttgat attgttgttg tggattctgt tgcggcactt gttccaaagg ctgaaatcga
    41701 aggctctatg gaagatcagc aaatgggact tcaggcacgt ttgatgagca aggctttgag
    41761 aaaattaaca ggtgttatcg gaaaaacaaa cactacagta atttttatca accaattgag
    41821 gatgaaaatc ggtgttatgt acgggaatcc cgaaaccaca acaggcggaa acgcgcttaa
    41881 atattatgcc agcgtaaggc ttgaaatcag aaggacagaa gggcttaaaa aagacgccga
    41941 agatatcgga aaccatgtaa gagtgagggt tttgaaaaat aaagtcgctc ctcctttcag
    42001 acaggcagaa tttgacataa ttttcggcaa aggcatctgt aaaatcggaa acatcattga
    42061 cgttgccgtt aatttggata taattaaaaa agcgggtgcg tggttcagtt acaacgatga
    42121 aaaactcggt caaggaaggg ataaagcaaa agaacttttg gagagcaacc ctgcacttct
    42181 tgccgaaatt gaaacgcttg tcagagaaaa acttgcccag cagtaacatt gaattataat
    42241 aggattatct tttcttggat tgccgcgtca gtcgccttgc tccttcctcg caatgacggg
    42301 ctgtgtttgc attgttgtca ttgttaggga taaatgatcg gattattact aaaatcgttc
    42361 acgtcgttgc gaggcgaagc cgaagcaatc taaaaatctg ataataggta caaatgatta
    42421 agtttgattt gtttacttta gaagcttttg tgtcggaaaa caaagatttt ctgataaatt
    42481 cgcgcataca aaaaattcag caaccgacca gaaaagaact tattttacat ttaagaaatc
    42541 aaggtgaaag cagaaaattt tacgtaaata caaatcccga gttttatcat gtctgtttta
    42601 tgtccaaaga aaacgagcaa aaacgcctta ttgaaattcc caaagctccg ccgatgtttt
    42661 gcatgctttt gagaaaatat ttagaaggtg caaaaatcgc aaaagtagaa caaccaaaaa
    42721 gcgagcggat tttggagttt tattttgagg tttacaacga aatttcggaa aaaatttatc
    42781 tgtgccttgc aatagagttg atgggaaaac actcgaatgt tgttttgtat aaccatgata
    42841 caaatgtgat tataggctgc cttcataatg tgggctctga taaaagccgc caaagggagc
    42901 ttgtgggcgg gcttccttat gtgtaccccc ccaaaagttc ggggggaaag tttttgtgtt
    42961 tagccgataa ttttgacaaa aaattgtctg tgaatgaaaa cattgacaat tattttgcta
    43021 atttgcaggg cgaaaaaaaa tttaaaacat taaaatcaaa acttttttct tattttaatt
    43081 ctgatttgaa aaaaattaat aactcgcttt taaaacagca aaagcagatt tcatcagatg
    43141 aaaaaaccca aacttaccgt aaaaaagccg atttaatcat gagtgggtta aatgagatta
    43201 agaataaaga ttttttgaca ttcattgaac ttattgactg ggaaacgaat ttacctgtaa
    43261 aaatcgaatt ggacaaaagc cttactctaa aagaaaatgc caacaggtat tacaaattgt
    43321 acaacaaggc aaagactgcc cattcaaaaa ccgaagaaat gacagcagaa ttaaagcgtg
    43381 aaaaagatta tctggagcag gttttgtact cggttgaaag tgtccaaaat attgatgaac
    43441 ttttggaaat cgaagaagaa acagaacaga aaagagggca agaaggcaag aaggcaggag
    43501 gacaagagaa gaaaatcttt tcacctattt accctctcac tcttcactct tcacccttca
    43561 ctatttatat cggcaaaaat aacaaacaaa acgattacat cgtgtcaaaa ctggcaaaag
    43621 atgaggactt gtggtttcac gttaaagact gcgcaggctc tcatgttctt ctaaaaaccg
    43681 aaaaacaaat tccttcagat gaaattattc ttaaatgtgc aaaacttgca aaagaatatt
    43741 ccagtgcaaa aaattcaacc aaagcggcgg ttatttacac caaagccaaa tacttaaaaa
    43801 aaccgccaaa agcaaagtta ggctacgtta ccttcactaa cgaaaaagag attgttatag
    43861 attaattcct aatatctgcg gcgagtttgt gggcggctaa tccttccatt tgcgctaatt
    43921 ttgatgcgca gggcgaaatg agttttttgc cttgaggaga aattatctga tatgtctgga
    43981 tttttataaa atcaaaaacg ctcaagcccc ctgaatattt tgaaacttcg tttgtgggca
    44041 aaacgtggtt tgtgccagag cagtaatcac cgaaaacttc tgcagaaaga tttcccacaa
    44101 aaagagagcc gtaatttttg aacttattaa tatatttttt tgcatcttta aagcagattt
    44161 ccaagtgttc gggtgccttt ttgtttgcaa tctcgattgc ctcgtcaatg tttttgacta
    44221 taacagcata tgatttttca aaagattttt cggcgatttc ttttgtcgaa agctcatcaa
    44281 gctttttttg ggcaaagatt tcgacttttt tggcaaaatc tttatccgtg cagattaagt
    44341 aagctcttgc atctttatcg tgttcgcact gggcgagcat atctgctgct ataaattcgg
    44401 ggttggcact ctcgtctgct attatcataa tttcagaagg tcctgcaaga aaatcaatcc
    44461 ccacctcgcc gtaaacgtat ttttttgcgg cagtgacata tttgtttccg gggcccacga
    44521 ttttatttac tttgggaatg gtctgcgtgc cgtatgccat ggcagctatt gcctgaattc
    44581 ccccgatttt gtaaatttta tccgcacccg ccatatcaca tgccacaatc acttcgggct
    44641 taatcttggg agaacatgcc acaatctcat ccactcccgc aacgtttgca ggtacaatcg
    44701 tcataacagc agaacttata agcggataat tcccgcccgg gatataacat ccgacttttt
    44761 caaggtggat aattttgtgc cccagctcat ttttatctat tgttgttttt aaacttttta
    44821 atgactttaa ctgctgtttt gcaaaagttt caacgttttt aatagatttt ttaattgctt
    44881 taatcgtatt tgcatcgact ttttcgtacg cttcaagaat ttcttctttt gtcaccaaaa
    44941 atccgtccaa aatttccttg tcgaattgag cgtggaattt tcttatggca ctgtctcctt
    45001 tttttcgaac ttcctgggcg atttcgtcaa cagacgaaat cgcctcaaat ttgatttttt
    45061 caaagaaatt atctgtcaga ttttcgtatt ttattatttg catattattc cttttttatt
    45121 tgtcgttgcg aggctttagc cgaagcaatc caaataattt taatttttag attgccgtcc
    45181 tgaaacaagt tcaggataaa cttcctcgca aagacgagaa tgttttgtta cttacttctt
    45241 gatgccaagt tgtttaaaat ttcctgcgga gttacgttgt tttctgccat aaatgtcagg
    45301 atgaagtagg ctaaatcggc aacttcccaa ccgagcccgt cgccctgttc aaagtttacc
    45361 acttcaaatg cttcttccat gatttttctt ttcaagagaa atttatcttt gaaaagtttt
    45421 gtagtaaaag agctttcagg catgttttct ttccttaatt ttaataattc aaaaagctca
    45481 aatacactga attctttatc ttcaaagcag ctgaatctgt ccagatgaca ggcgtttcct
    45541 ttttgtttaa ctttaaaaag aagcgtgtca aaatcacagt caaaccgcac cgtttgaagt
    45601 tcttgaatat tgcctgacgt ttcgcctttt gtccaaagct cttgtctgga gcggctgaaa
    45661 tatgttgcaa gccctttttt aaaagttttt cttaaagatt tttcattgga ataagcaagc
    45721 atcaaaacct gttttgtgtc aatatcttgg acaatagtgg gaattaatcc ttttcccttt
    45781 tcaaaatcaa ggcaagcaag aaatgcgtct tcgagtttaa ccgtatccgt gtaaatacac
    45841 attcctaact gtgttgaaat gtttttgcgg ttaagatcta ctatttcttc aatagagcaa
    45901 atcccgcctg ctgcgacaaa ttcttttttt gttgcctttt tcaattcttc gattgatgcc
    45961 atatcgcagc cctgcatcat gccctctttt tccacaacgg tataaagata gcccgaacaa
    46021 agtttgtcaa agtgtttgat gaaatcgagt ggggttttgt ccagctcttt tgtccagcct
    46081 tctgttgtga ttcttccgtt ttttgaatca atcgccacaa taaccctgtc tttgggaagc
    46141 tgcgataaaa attcttcgct tgctgctgtt cctattataa tttttttagc acctgctgct
    46201 ataagccttt ttgctttttc tacggttctt atcccaccgc cgactcggca cgatgcaatt
    46261 ttgcaaagct gttttattaa ttcttcattg tcatttccgt ttcccatagc acagtcaagg
    46321 tcaacaactg caatatcgcc aaatcttgaa tagtattttg caagttcaaa gacgtcttct
    46381 ctttcaagaa ctttttcttt accttgctta agctgtactg ctttgccgtt catcaaatct
    46441 atgcttggaa tcaacataat attttccttt caattatttt ttattatagc ttaaaaaata
    46501 tttttgagag ataattgctt tataaaataa ggagaattta tgaaagcggt tgtatttgac
    46561 ggagaattaa aactggttac ggactacaaa aaacctgagc ccaaagttgg tgaagctttg
    46621 ataaaagttt ctatggcagg aatctgtaac accgatttgg agcttacaaa aggctatatg
    46681 ggatataaag ggattttggg acatgaattt gtgggggtgg tggaagaggt aaatgataag
    46741 gataaatcat tgttgggtaa aagagttgtg ggagaaataa actgcggctg caataactgt
    46801 gaatggtgtc ataaaggctt gcaaaggcac tgcccaaacc gccaaacttt gggaatttgg
    46861 caaaaagacg gctgcatggc gcaatacgtt tgcatgcccc ttgatactct tttagaagtg
    46921 cctgaaaatg tttcggacga gcaggcagtt tttgttgaac ctcttgccgc aggtttggag
    46981 attttagagc agcttcacat tcagccagtt gaaaaagttt tgattttagg cgacgggaaa
    47041 ttgggtttaa caacagcgct tgctttaaat gtaagtaatt tggatgttct cttggttggc
    47101 aagcatcaaa ataaattgga tattgccaaa aatcagggag taaatgttaa gcttctttct
    47161 gatttagtga ttaaaaaaga atgggatttt gtggttgagg ctacaggctc gatttcaggg
    47221 tttgaaacgg ctttgaattt gacaaaaccg cgcggaacac ttgttctcaa aagtactgtt
    47281 gcagcgtcaa aagagtttaa ccttgcgccc attgtaatcg atgaaataaa agttcagggc
    47341 tcccgctgcg gtcagttcgc tcctgcattg aggcttttga aatcagaaaa aattgatttt
    47401 tcgcctttga tttctgcggt ttattctgtc gataaggcat tagaagcttt tgaaaaaaac
    47461 aaagaaaaag atacattaaa agttttgtta agattttagg gggtaaatat atttggctga
    47521 atggctgaat aggtgaacgg ctgaaaggtt attttagttg aaaagtcgaa tagtcgaaga
    47581 gttgaagagt tgaaaagttg aaaagttcat gagcttgtaa attcttctca cttctcacta
    47641 tttaccattt actatttact tattccccta tttccttttt ataatgtttc atctaaaata
    47701 gtcttatggg tgtgaatttt attaaaagat taaccatttt ggataaatat atcttgaggc
    47761 aggttattga gatgtttatc atgggcgtgt ttgtttttac ttccataatt tttgcttcag
    47821 atacttttat aacacttatt aagcaaattt cactttacgg gattcccttt aaagtggcat
    47881 tgatgataat tattttgaac ctgccttcgg tttttgtaat gacaattccg atgggggttt
    47941 tgcttgcgac cgtaatgact cttaacaggc tttctttggc atctgaaatt accgttatgc
    48001 gctcttgcgg aataggaatt aacaggatag caaaacctat ttttatattc gctgctatta
    48061 tgtgtcttgc aagttttgcg attaacgaaa cggttgtacc tgtaatgaca aaacagtcaa
    48121 aggatttggc cctgtgggct ttgggacaaa aaaacatccc cgagggcaaa cagaacttta
    48181 tttttaagga attaagagat aacggaacac ttaaaaggct tttttatgta ggctattgca
    48241 aagataaagt gcttcacaac attaccgttt tggatttagg aaaagatgat acaattcagg
    48301 tccttcaggc acaagatggc ggcacctctc cttcgggctg ggaatttaac cgcggggcta
    48361 tttatactgt cgctaaagaa ggcaaaattt taaatacgac tttctttgaa cactctatag
    48421 cacagtttgg gatagattta tcaagagagt taaatcaaaa tgttgcaaaa gagcataatt
    48481 ttacctcttt gattaaatac atgatagaaa atcgtgataa aatttcggag caaaaaaact
    48541 tttatctcat acagcttttt gataaacttg cgcttccgat taccaccata gtgcttgttt
    48601 taataggtgt tccgcttgct attaccccgc ctcgggtaag atacaacaga ggatttttgt
    48661 tcagtatttt gataattttt acttattatt tgattagagc cttgtcgctg tcttttggtg
    48721 aagcgggctc actgcatcct tttattgcag catgggcagc aaacatcctg cttactcttg
    48781 caggtgttgc gctttattac agaaaagttt atacaataaa ttagccttta atacttagtt
    48841 ttggcgtttt tccgtttgtc attaaatttt ctctgttcat tgccatggtt gtattaaaag
    48901 cagctatgtt gtaatactct cttagttttt cataatcttc actttgcaca aattccacct
    48961 gatcaaagaa attatttaat tcttcgttag gcttttcaag tgcttcgctt actcttgcac
    49021 cgatattttc cccaaacgca cgaaaaccct gccccatgct accccaggtt aaaccaaatt
    49081 gcccataatt agatgtgtta acagaatttg acatttttat cccttaattt cgatacttac
    49141 aataatttaa tcggaataat cacacctgaa gtttaattat ttaagggtaa aagtacataa
    49201 agtttacaaa aatttacgtt taatcgtttg aataaattct cacataattc caataactct
    49261 tataaacttt tttgagataa ttttgggttt caaggtaggg gatttgttct acaaaatcat
    49321 cgatgtcggc aaaatttata tcttctttcc atcttgaaac agagcctatt ccaccattgt
    49381 aggcaagaac agctaagatg tctttatcaa aaagcacttt tctgagtctt gaatagtaaa
    49441 tatttccgat ttttatgttt atgtcgggct ggagcaaaaa tttatcactt aaaagattaa
    49501 ttcctctgtc ttttcccact tcgcttgctg ttatcggcat aagctgcata agccccattg
    49561 cgcccacagg gcttatgctt ttggggttga aatagctttc ttctcttatt attgaaagga
    49621 ttaatacagg gttgttgtta aatattttgg cgttttcttt aatctcttta taaaaatgaa
    49681 tcggataaac aagtctccaa cgggggtctt ttctgtcggg tttagtttca agtttatcca
    49741 tagcgtctct tgcaattctt gcagaagaag aaaaatctcc ctgttgatac ttaagccagc
    49801 tttgaataaa atcgtcatct ttgcaaagtt cgtttataag tccccagtcc ttgacttgcg
    49861 cgagttttat tattaattcg ttttcggaat tttttttgta agggaataaa atttctttat
    49921 tttcaagttc ttctgccgtt aaacttagat ttttaaaccc gtttaaattc agataggaat
    49981 ggtatgcgta ataatcatcg ggaaattgca gaatgagatt tttgtaaaaa tttatcgcaa
    50041 tgtcataatt ttttgtcttt tcattaattt ttgcaagcca aaacataacc cttggggtag
    50101 agtttgtttt cgggaaattg ctaagatgct ctgagcctat ttttttggct gtgtagtagt
    50161 ctcttgtttt gattttttcg taaaaaatat ttgaaagtga ttctgccgcg aattgacctt
    50221 ttgggtattt gcggtatagg gtgttaaagc aagctaattg aagattttgg ggcatgttaa
    50281 gacaattttt gtaaagcaga taatcatgac cgggcgcatt ttcagacaaa ctcaaaagat
    50341 atgatataga tgtttttggg ttttggctta ttgaaagata agtgtcgatt gcatcataaa
    50401 cagccttatt atcgattgtg tcttcagggg cttcatttat tgcaacagca ttaacaggag
    50461 gctcttttag tccgaattgc gtgtaatatt ttacctttga ataatctttc attgaataag
    50521 cgttttggac aaaataaggc catgagatgt ttatgtttgt gtaattaaga tatttttgcg
    50581 catttttgaa atcgccattt gccatgtagg atttggctat tatcagatta tcttcgtttt
    50641 tgagtttttt atttaataaa atccattttt ctatacaatt tattgaaaat cttcccgagg
    50701 gtgcttcttt gagataattt ctgaaataat gtgaagcttt tttaattgtt ttatttttct
    50761 tttttttgtt ttttactctt tcgatatgga gggtttcaat cgagcccatg taatagcctg
    50821 aagcaagcgc gtaatcactt tgggggtatt ttctaataat ttctttgaat tctttttttg
    50881 cttttttata gcttttttct tcatacaggt actgcgcttt ggaatatttt gccttaaggg
    50941 ctatttttga atttttgtat tttttgatta tttcgttata ttttttaatg gctccttttt
    51001 tatctgaaag ttgctcggca caaaaagctt gtctgtaaag tgctgccggt ttgagttttg
    51061 atgtttttgg tattttttca aaatcggcat aagcttggga taagtcgttg tttttatatt
    51121 tttccaaagc ttgggtatat tttttgttgc ttatactctc cggagtccaa attttcggag
    51181 tatttataaa agcaatcgca atcacagcaa aaattataat tgcaataagt gttatttttt
    51241 gtcttgatat catatttttt accccaaatc aaattttatt gtaacaaaaa atattcttat
    51301 aataaatatt ttgtctaagg ctttttacaa aaaaaacgtt tattgttgca aaatgagcga
    51361 aaaaattttt aaaacagatt ctaaaacagc acaaaagagt aagtactttt ctatgcccag
    51421 gcatgatttt gtctttcaat cgagaaaaaa tcttattaaa aattacattc ttgaccctga
    51481 agtaaagctg gttaaataaa tttatatttt taaggcttcg atttcttcga cttctttttt
    51541 atttagcaaa accgctccgc ctagaagttt tgcgtttaat actacctgcg cataagattc
    51601 tgcaagttct aactttaaat aagcttgttt taaattaata tcgcccacta taaatccgtg
    51661 atttgccatt aaaaccgcat tgtgtttttc aaaatatttt tcggtttttt caacaagttc
    51721 ttttgatgaa ggaagagcat aaggcgcaag aggaattttg ccgaaataat aaacattttc
    51781 tgccataacc ggttcttcca agccttttcc tgccgcggca aaagaactta aatatgacgg
    51841 atggacatgg attattgcat ttatatcgga cctttttttg taaaaagcaa cgtggagcat
    51901 tttttcgctt gaagcttttt tgtctccttc aataactttt ccttcaaagt ctattacaac
    51961 taaatcttcc caagccaaat caccgtttga agagcctgac gcggttatta ttattttatc
    52021 tttataacga acagaaatgt ttcccgaggc tccgggtgaa aatccttttg catcaacaat
    52081 atccccgtat tttatgattt cattttttaa atcgtttaac attaaaacct cttttgattt
    52141 taaatagttt cattgtcatt tttaatttcg ataacatcag ctctttctat gcctgaatca
    52201 tcatcttgtg ttgtaggagc aaaagtgttg ttagggttgt ttttgccgtt tttttgtttt
    52261 cctaaagtgt cgggattttt gatttccatt cttttatatt caatagtaga gccttttgca
    52321 tcaagcgcaa gagccattgt aacaaaggat tgttgcctta ctgtatcata tcctacatga
    52381 agctttatgt cgtcggggcc caatgaaaag aagaaagaat tttcctgcat taaagagccg
    52441 ttccatgcgt cgtttgttaa gtttacagag ccaaaccatg atagtgtgag atattttgta
    52501 agccttaatg tttctcttac ataggcattg gagcgtccat acatgtattt atcaaataaa
    52561 ggcatcggag tattgtcgtt gtagccggat agaaaatatc cgacatcttg catccagtat
    52621 ttatattgag tatgaagtct tggacctact cttgcaagca tttgggtgtc gcctgttccg
    52681 taaagtgaag cggcaccttg gaataccatt tcaagccttg cggccaaaat atcgtctttg
    52741 tttttaattg tttttaaaga cgcatcttgc ataaatcttt cgcaattaaa attgtaaatt
    52801 gtttgagaaa cttcggtcat atattttaat cttaaagttc ctattccgcc ttcgcttttt
    52861 agtgctccga tgttgccgtc accaacatca ccgtcttgca tgtaacctgc tgcaaatctt
    52921 tgtgaaaaca tcatatcaag atttttgcct aaaaagtttt cgtttaaaac tctgtcatcg
    52981 taatatactt cagccatata ttttggcatt ctgaagccca taaaccagtc atccatataa
    53041 gcatttgatc cgtattgcaa atataaattg tcatcaagtc tctgtttacc tcttactata
    53101 aacatttcac cggctgttcc gtaggcgata tcggttttat ttgttgcatt tttgaatttt
    53161 gcaattccgc ctgcaccaat tttacctgcg ttatagttaa acataggaat aagttttacg
    53221 gtagagcctt tcggagtatc aaaaacaaaa cccggtcctg cataaaagcc caaatttgtt
    53281 gttgttccaa gttcaggata atctgcttcg acgtaatctt ggttcttgtt tgtatgcacc
    53341 gtgattgaag ggattgttcc gatttttttc tcgtcaaaat aaatttctgc atgttttaga
    53401 gttaccgtgt cgtgctcatc tttggagtta attattaaat cattggtttt gattttgaat
    53461 ttttctccgt gagaatcttt tttataaaga gctttatctt gatttgcaag gtacattgta
    53521 cttaaatcag gtccaaacat gtctgagcga agattaatgg gcattttctt tttaatataa
    53581 agacttcctt gctcttggat tattttatcg ccgtacatat agcccatttt agcttttgcc
    53641 tgaatttgga aaatgtcgcc ttcgggtttt tccaaaaatg cgttttcttc atttaaatcc
    53701 acctgcatgt aatcgccggt taattctttt ccgtcgctta ttaaaacaac ttttccgaat
    53761 gctttaatca ggttggaggt ttggttataa atcattttgt cggcttttat ggtggaattg
    53821 tttttaggga aaaataatac aacgttaccg tttgcttcaa gctctgtcct gtcggcaaaa
    53881 tattccatag tattgcaatc caacaacgct tgatttttgg aagcggttgt ttgggcggaa
    53941 gtttcttgtg tttttttacc tttatttttt attgcaagcc gaattttttt tacaggaggc
    54001 agtgttccgc gcatctgccc ttcgccgggt acttggtcgg ttttagaaat ggattcttct
    54061 ttcagttttt ttaatctttc tttttcttgt tgggttatta cggatgtaaa aaagttattt
    54121 gtataactgt taatttcgga aaaagaatcc atagtttgct ttttgctgtc gatatcagaa
    54181 ataaccttag gctcttgtcc cgcaaaggca agccctgaag atgtcgctaa aactattaat
    54241 aataaaatca gatttttttt catagattct ttctatatgt aatttaaagg attattgggc
    54301 cttccgttaa ttctaacttc aaaatgtaag tgaggccctg tgctgtatcc tgttgtgcct
    54361 tcatatccta cgacttgtcc tttggaaacg tattctccga cgcttactcg ggatgaattt
    54421 aaatgggcat ataatgttgt ggtaggagtt ccgtttactt gtccatggtc taatataacg
    54481 acttttccgt atcctccgta ccagcctgta taaatcactt ttcctgcatt tgaggctctt
    54541 atgcttccgt tgtaaggccc gcctatatct atgccggagt ggaaactcct gctgttaaat
    54601 atcgggtgcc ttctccaacc gaaaggcgag gttattcttc cgcttatggg ttttataaat
    54661 cctgaaacaa tcctgatatc tgaagcatga ctccctcttg aaatcattgc ctggatgctt
    54721 tcggattgtc tggcaagctc tctttctgcc tgctcataag ttctgcggtc agtccttaat
    54781 ctgtgaatca tattttcgtt tcgggcaatg tcttgctgaa tagcttgttg ctgataattt
    54841 atatttttta tagaacgcga aagtactctt ttttgttctt caatattata cttcaaaagc
    54901 gcaatttctt tagccttcat tctggcataa ttcattctgc gatagtcgcc tcttatgact
    54961 attccttcaa aatagagcct gtccaaaaag gaattaaaat cctgtgcgct taacagtaat
    55021 tcaaaaaatc cccctctttg gcttttgaaa atctgtctta ttcttctttt catctgaaac
    55081 tctatttcgg aaaaatcaga caaagatctt gataaatctg tttctaaatt acttaacttg
    55141 ctttgagtag tgtttaattg ctgtctggat tcaaccagcg atgaagttgt tctctcaagt
    55201 ttttgctgat ttttataaag tttatttgtt tctaagtttt caagccattt taaatggtta
    55261 atttttgctc ttgtctgctg ttgtttttgt tgaagttccg tgtttgcaaa acaaggcatg
    55321 gcaacaaagc tcagcattgc cgttgcaaag aaaaaaatcg caaatatttt aaatatattt
    55381 ttattcaccg tttaccttgt tcggcttata cccataccgc cggctatccc tcagcctgta
    55441 tgggtttagc acttcttagt tcggatttga aaaattacaa aaaattgtca ttttttgaat
    55501 ttttcctcat cctctatttt attaaaagag gcagcatcat aagccctaca gtccatgaaa
    55561 tgaatgtaaa agctattatt gcaataatta tcttctgatg ttttctgaaa aattccataa
    55621 tcctctccaa tgataatatt ttactattaa accgccaaaa gtgcaagagg gggtaaatgt
    55681 atttagctga atggctgaac gggtgaaagg ctgaaaggtt attttagttt aaaagtcgaa
    55741 tagtcgaata gttgaagagt tttatattag tcgcgaagcg acaaattgga cttattcact
    55801 tatttaccca ttcacttcta tttaccattt accatttact atttactgta taaaattgcc
    55861 aaaatgtcaa aaatattata gaattaattc atgaaatata attttttaaa tatttttttg
    55921 gataaagaag atttatttaa tcaggaaaaa ttttccatcg ataaattttt gcttgaaaac
    55981 aattattccc aaattgaaaa aatttataat ttttataaag aaacatccaa tttattgtac
    56041 gtaagcggtt ttttaggaac cggcaaaacg caggtcatta attacactgc aagtttttta
    56101 tcggcagaaa cagtcttgtt gagttatttt tgttcaagtt caaccattct ggatgatatt
    56161 ttgctttcgt tttttgcaga attcagaagg cttgcggata aaaaattgat tgttgagcct
    56221 aaagttaaga ccgaaaattt tgccgagaaa ataaacgtat atttttctca aatagaaaga
    56281 ccttttgtta taatcttgga ttcttttgat tcaattttat ctgaaaatcg gcaagaaatt
    56341 ttggatttta taatgcattt gtgcaaactt aacaaagtaa aagctgttat tatttctaaa
    56401 acttttgagc aggaatattt taacaatcct tctgtgaggc ttgaaaaagt tgctctaagt
    56461 gctttttctc aaggcatttt tgaaaaatat ctcaagtccg aaggcataaa acttccgcct
    56521 aaaattttgg aagaatttta taaacagaca agggggtata tgttttttac ctcgcttgta
    56581 attaatttaa ttaagtcaaa aaaccttgag cccattcagt ttttaagaga attcagcgaa
    56641 agttttttaa ctcttgacga gtttttgtca aggcaaactt tagggtttat ttcttccgag
    56701 gcaaaaaatt tattttggtt tttatcaatg cttcgccacc ctgtaaatat tgagctgcta
    56761 aaaatattag atgtttatga cgaacaaaaa atgaaaaatt tgaaagataa ttttattctt
    56821 gttaaaaata attcttcggt gtatattcac gagtttttta aagacaggtt tgatattgag
    56881 ccgcctgcca atattgtaca aaaaatgcat caatacattg ttgatattta caaaacacaa
    56941 cttcctctaa aaccttttga gcgaaacatt ttaatttcaa ggcaaaccat gagaaaagaa
    57001 atcaaatacc atgaattttt ccttcctaaa aaaataaaca cattagagga taaaaatatt
    57061 gatataggct atttgtctta tgcaaaaggt cttgaatttg atttgggtca tccgacgtcg
    57121 gcaagcagtc aaagtgttgt ttctgctcct ttttcggaga ctaaaaaaga ggtggaagta
    57181 aaaacgttta accctgaggc aaaagataaa actcgcattg tttctcaaat ttttccccat
    57241 gaaaaattac aaaaagctga agaagttaaa aaaataagta tctcggattt gataaaaaac
    57301 attaaagatg caaaaagggc ttataattac ggattggtaa tcgatttttg caaagaatta
    57361 cttttgcgcg aagacactct tgaaaataaa aaagatttac cgtttattta tacggagctt
    57421 gcttttgcat accaaaaaat ggcagatatt gaaaacgcac ttaagtattt taatcttgct
    57481 caggaaaaat ttttacagca aggaaaaaca acagaagcaa atgaaataaa acttacaatt
    57541 tctaacatat ttattgaaaa ttataaaaca gaacaggcaa aatttatttt ggaagatatt
    57601 ttaaagtgcg aagaaattac agatatctta agaataaaag ctttaatgca tcttgcagtt
    57661 gttgaagaca acctttctaa tacccaaaaa acatttgaat attataaaca agcggttaaa
    57721 atttcaaaag acgttattga taataatgtc ttaatggagc tttattttcg ttttgctgcc
    57781 tttttggatg ataataatga aactcagctt gctcaagaat attatttaaa atgcattaat
    57841 cttgatttgg aatcggataa taattttgtt gctcttgcaa attcaaatct tgctgatttg
    57901 tatctggaac aggaaaattc aaaatctgca agggaacact attttagggc ttttgaaatt
    57961 tataataaaa acggtaatta tgatggatta tatcattctt cgtcaaagct tgccgttatt
    58021 ttaagtataa gaagccccca ggaaacactt aaatatcttg aagctgcatt tgaaagcgca
    58081 agttttttaa atgacaaatt ttatatggca tctgcacagc tggccttggg cgattttttc
    58141 tatgattcgc ttaatgatga aatggctatt aaaaattatc tcggagcatt gaaaattatc
    58201 gaaaaagaat ctgataaaga aaattccgat aaaattcagc taaggcttaa ggatgtaaaa
    58261 ttaagaatag gtgaagaaaa atttattgaa cttataaaca ggtttggcaa tgaatgattt
    58321 tggagaaaaa acggcgatta ttgaaaaata tgcgcgaatt tttcttgaaa aaaattcgcg
    58381 cctcaatttg atttcaaaaa acgatgaaaa agtttttctc aaaaaacatg tatcagattc
    58441 tcttgcaata gataaatttt ttcaaaaaca taaaataaaa aaaggtcaaa aactccttga
    58501 catgggaaca ggcggcgggc ttccagctgt tcctattgca attttttatc cggttttgga
    58561 agtttttgca atagacagca taagcaaaaa aattaatgcg gtggaagaaa tcaaaaaaga
    58621 acttgaatta gaaaatcttt atccgatttg gggcagggtt gaaaatttac cggcgcaaaa
    58681 ccctgaaaag tttgattatg ttgttacaag agcacttgcg cctttggata aaattttaaa
    58741 gtacgcaatt cctaatttaa aaacgggcgg atattttatt gcatacaagt caaaaagagc
    58801 acttgatgag attaaagaag cccaaaaaac tttaaaaaaa tacaatttaa aagttgttga
    58861 aatcattgaa tatgaattaa aatcagaaca aacgtatcaa agaaatctga ttgttattaa
    58921 agtgagtaaa tagtaaatgg taaatggtaa atggtgagag gtgagaagtg agaagtgaga
    58981 agaatttaca agctcatgaa cttttcaact cttcgactgt tcgacttttc aactcatcaa
    59041 tccaaataaa aagcccgaaa aatttcgggc gaaaatcttt tgaagggaag gtagggtagg
    59101 ttggggtgta gggggtttgt ttttatgtgt aaactttgaa aggagttagg cgattttcag
    59161 gattgacctg ttgttgaatg caatcatttc catatattca aataaatctt cggagcggtc
    59221 aacaaaagga gcttgaacaa ctttttcttc ttggattttg tctgttgcat cttcttcgac
    59281 ttcttttttg acaggtttta aaatcatcgg atttttgcct cgtttttgct ctcgtatata
    59341 taaagtatat accaagagtt ggatttcagg tttactgaaa actgttacat aaattaatat
    59401 ttttaaagct tttttttaac aatttttggt tcaaaaatgt ttttatataa taaaggtagg
    59461 aatacagtaa agttatgaca tatccgatta atacaaagtt aaatcaatat ccgcaggggt
    59521 tttcggcaac acccgcaata cctgttgatg ctgaaaaagt taaacaaagc gttaatcaaa
    59581 atgggcttgt aaaacaggca aaagaagaag aaaatcctgt acttttgctt ggtgttatga
    59641 tgcctatttg ggcagctatc ggtcatttaa tggcaaaatt taattatgcc tgttctgatg
    59701 ctaacggaaa aaatattttg caaaaaatag cagattttgg tcacaatatc ggagaaaata
    59761 aggtttttaa atctccgggt gcaaaacatg caggtaatat tttttcaaat ataaaaaact
    59821 ttgctaaaac aaaaattatt gacaaaagca gtatattgtc tgcaatgttc aaaactccta
    59881 ctgcacctga atttcagatg gcaaaagtga tgtcaaaagg gactatttct gaagttgcaa
    59941 cagatgcttc tcaagctttg gatgtttttg ttgctcagca caatagtttg gacgacgcgc
    60001 ttaaagcttt aaaaattgat aaagtattat atgaaaatat taaaaaagat ccatacaaac
    60061 atatagacga tataattgat atttgcaaaa aacaaaaagg cgatgctttt gtaatgctta
    60121 aaaaattggg tagaatgcct ttgccaaagt ttttgcaaag aaaagtttat ttttctgaac
    60181 ttgcaaataa attaactttc ttaaaagggt taaaaaatcc taaccctaat cttaatgtag
    60241 tcggtaaggt tttaccaaaa atgagcatca ggactttaga agggtttaca aacggtacag
    60301 ctggaggcaa atttgccatt ttgatgcagg cttacatttt tgcatcagca cttataaaag
    60361 cagcaaaagc tccaaaaggt caaaaagtaa agactttttc agaaaatgta ttttatgatt
    60421 taggatttta tttaacaatt ccttcaagta tggcacttat gcatggagca ggagggctta
    60481 aatatatcgg aatgagtgaa aaacaagtcg atttttacag aaaagctttg gaaaaactta
    60541 atgcaagtgt tgatgccgga acaataagca aagtcaaata cttaaaagcc agaaaagatt
    60601 taggaatttt attaaaagga aataccaaaa ttttaaaaag cgataaattc ttagttagaa
    60661 gcgcaaaaca tcttaaaaat attgtttata atcctttgaa atgggcgggg aaaatggttt
    60721 cagtcggctt agaaagaata aaaccttatg ccggaaaaga tgcaaacgct atattaaaag
    60781 gttttggtaa atttaaatat aagcttaaag gcgcagcagg ggttcctgca aggtttttac
    60841 ttttcagtat ggtttttgct ccgtttcttg caaaatattt tgcaaaagca tctcatctta
    60901 tattcggcag acctacaaaa tcggtacttg atgatgacaa agagtcaaaa gcacccgaac
    60961 aaattaaatc gcaaccgaca aatcagcaag gggcagtgcc tacatcttta atcactcccc
    61021 ctgcacaaag cggtaattta gtaaatatgt ataatcagca agcaaataaa cctgttcctc
    61081 aggcagttca gccacagcag cctgcttctc aacagcaaat tgcgcctggg caagctttgc
    61141 agcctgttgt tcagcctgcc gctgtgcagc caaacactga aacctctttg gtttatccgc
    61201 ccaaaacagc aaatcagact ccgcctgcac ctgcaagaac ttatgttcct tcggcagagc
    61261 ctataaagca agaaaaaact caacaaatga cagaagctga gcaaaaactc gcacttgcgc
    61321 tccaaaaagc agattccgcc gagcagtacg ctaaaaaata tgcaggataa taattggttg
    61381 aataggtgaa gagctgaaaa gttataaaat tgatgtcatt gcgagcgacc aacgggagcg
    61441 aagcaatcca aaagatgggg aggtcgggga atatctaccc taatttactt attaaatttt
    61501 tataaaacca ctggcaaatt attttttttt ggggtttaat ataaaaaata cagaaaaaat
    61561 tttatttgaa gaaggaaaaa ttttgaatac gattttgaat accgatttag atactgccca
    61621 aagaaattgt gtcgcaagcc caattcataa aaatcgtttt caaaaattag agacggcatt
    61681 aagagattta ttttataagg agttgaattc aagcgactct atttttttgg aaatttcgcc
    61741 tttattggtt aaaaaagttg cgatgttttt atcggggctt gctttcagac ctgcagcgat
    61801 tggaatttgc ggggaaaccg caagcggaaa atccactata gtaacagatt caatagatgt
    61861 cataaacagt tttgcgcaag aatttattct agaaaatgtc gtaactacaa ttaacaccga
    61921 cgattactac tatgaccgct caaaagaagt ccaaaaagcg ggttcttttg ccgagtttgt
    61981 aaaaacttac gatttagatg ttccgcaggc aatggaattg agcctgatga aaaaacacat
    62041 tcaagctctt ttaagcaagc aaagcgtttt gctccccaaa tacgatatgt cgggaactgc
    62101 aaaaagattt gaaaatcaca caaaggctaa tccttgttct gtggtaattt cagaagggct
    62161 ttttacttta accgatgaaa ttgcggatgt ttttgatttt aaaatttatg ttgatataga
    62221 taaatccgtt caaaaaagaa gattttacga aagggcacaa aaaagagggc tgggagactc
    62281 tgccgacaga atttttcaaa atgcaacaga taaggcagaa atttatataa gaccttgcat
    62341 gaaaagagca gatattgttt taaacggtga aatttcaaga caaaagtata aacaatttgt
    62401 tgcttctttt cttaattgcc tcggtgagat ttattatggt ataataatgt aggcatttag
    62461 tttaaggaat aagtatgaaa ttatgcgtgt ttcagggaac ttttaatccc attcacagta
    62521 ttcatatggc tatggcaggt tttgtaaaga aagtttataa ctttgacact attttgttta
    62581 tccctgctta taagccgccg cataaggaat ttgacgatga tttggcaaac catagactac
    62641 agatggtcaa aattgcaatc gaaggcaaca cctctttttc tataagtaat attgaatttc
    62701 aaaacgaaag atattcttat acttatctta cgatagaaga actttataaa agatataaaa
    62761 tcgaagggaa aattgctttt ataataggaa tggacgcttt cagagaagtt acagaatggt
    62821 atgaggcaga caaacttaag acattggttg attttatagt tttccccaga gggcaggaga
    62881 ttgataaaga tagacttgcg ctgttaagat ataagggcta caattttacg attgcaaaaa
    62941 tggatgcggt ggatttgtct tcttctatgg ttagagaaag aattgtaaaa gggcagtctg
    63001 ttgccggttt aatccctctt aaagttatgg agtatataag agaaaatgga ttatacaaac
    63061 aagagtgaca aagagatttt agcttggctt gaaaaaaact tgagtcaggg gcgtttttta
    63121 cactctttgg gctgtgccaa gcgttcagta gagcttgcaa aaaaattcgg gcaagatgaa
    63181 aaaaaagctt atgttgcagg acttttgcat gattgtgcca aatcttttga tgtggaagaa
    63241 atgcgaaaaa taatccaaga taaaaaactt gatgttgcgt cttgtgaatt tgtaaatcct
    63301 aaaactcttc atgctccgct tagtgcttat gttgcaaaga ctgcttttgg agttgaagat
    63361 gaggaaattt tgtctgcaat ccgctggcac actttgggta gggttaatat gagtaagttt
    63421 gagaaaatta tttttcttgc agataaaata gagcctcaca caagaaaaac agagtatgca
    63481 cagcttatta tggggatttt ggaagaagaa aacggaatag acaaagcact tttggtctgc
    63541 tacggcgaaa caataaaaag tcttaaagcg cgcggtctgg aaatttgcca agtcacgatt
    63601 gacgtttata acagtcttca aagtttataa gaagacaagt aaagtaggtc ggcattgcta
    63661 tgccgacaaa attattggcg ggatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    63721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    63781 nnnngttttg gaattaatct catcaagaca aattccgccg gaagttacga tttcgccttc
    63841 ttttgtcggc gatatcgcat ttatttcaaa ttcagacagg gattttttaa ttaaatctct
    63901 tttttgggaa ttaatttgag agcatttgtc gtcaacctta atattattta gccccaaaat
    63961 gtattctgcc attgattttg gtacaaattc acaaagaaga tttttaatat tttttttaac
    64021 gtttgaattt aaagcaattt gcaggtcaaa atcttcgggg ataaattcaa gcttaatttt
    64081 caggggattt tgcctgttgt aatcaagtct tgcgcaaagg gaagaaattt tgtaaatcaa
    64141 aggccctgat actccttgat gtgtaaaaag tatgtcttca ttttcttttg tgattaatct
    64201 gtcattaaaa aatacttgag ctttaatgtt tttaaggctt aaacctttaa gcgaagaaaa
    64261 attttcattg cttattaaac cgacaagaga aggttttggt ttaatgattt tatgccccaa
    64321 actttgcgcc aaatcaaagc cggcatgccc gcctgttgca attactactt tgtcaaagga
    64381 gtaattgttt ttgtcggttg tgatgaggaa ttttgatttt tctttcccct cgccccttgg
    64441 gctaaagatt ttattataat tgctctccct ctccccttgc gggagagggt tggggtgagg
    64501 ggtgccgtag ggcgagggta gggtgagggg ttttattttt attacttttt cttttttaat
    64561 cgtgcaattt ttaatgcttt tgagcatttt ttctttgact tcttttgcgc tgtttgagat
    64621 cgggaaaatt ctcgaatctt gctgagtgta agtttttact cctatttttt caaaaaattc
    64681 tattgcattt gaggttgaaa attgtgaaaa aactgagtac aaaaactttt cgcctctggg
    64741 gtaatttttt gctaattctt taaaatcgta ttgggcgtaa gccaaattgc atcttcctcc
    64801 gcccgtgtaa agcagagtat gaagcggttg ggcataatca aacaaaatca catcggcatt
    64861 ttttgaggca aagtaagcgc acatgcatcc tgcaggtcct gcaccgatta ttgcaacagc
    64921 ttctcgctca ccgctcacag ctcactactc actcacttat agcatcaacg cactcagaac
    64981 atattgtttc atgcccttcg tgttcgccca gcgttctgta tttccagcag cgttcacatt
    65041 tttgcccttg agcctgagta acccaaacaa aaaagccttc ttctttgtat tcgtttaaaa
    65101 tattttgagg tttttgttcc gtcaaaaagg cttgagaagt tatgaaaata ttacaaagtt
    65161 cgtcttggtt tgcgcttaaa atttcatggc tttcttgggg tgctttaaca taaactgcaa
    65221 cctccaaaga acttcccact tttttgtcgg ctcgaagagg ctcaatcgct tttgtgacaa
    65281 tctctcttgc ctttaatatt gttgcaaatt cttcttctaa ttgagcgttt tcccatcgtg
    65341 ggttgaattt cacccaattt gaaagcaaaa tactttccat accgtctttt tggcattcga
    65401 taacgcttgt ccaaatgtct tctgcctgat gaggcattac aggagttaaa atcctcacaa
    65461 gtgcctgaga tatttcaaat aatactgtct gacatgcccg ccttgaaagg gattttttgc
    65521 ctgcggtgta gagtctgtct tttacgatgt caagataaaa tgcacttaaa tccacagccg
    65581 caaagttttg caaatactgg aagtatttgt aaaattcgta gctgtcaaag gcttctgtta
    65641 cgtcttttgt cagcctgtta agcttgtgca gtgcgaattt gtccagattt ttgagttccg
    65701 aatactggac ataatcggtt tgcgggtcaa aatcgtaaat attcccgagc aaaaaccgca
    65761 tggtgtttct cattttttta aatatttcaa caagctgttt tatgacgttt tcacctattt
    65821 taatgtcgtt tcggtaatcc acagaagctg cccaaagcct cagtacatcg gcaccataga
    65881 ccttgataat ttcttggggc gcaactacat tgcccaaaga ttttgacatt tttctgcctt
    65941 ctgcatcgag cacaaatccg tgggttaaaa cttcttgata aggtgcttta ctttgcgttg
    66001 ccatcgcagt cagaagcgaa gattgaaacc aacccctgtg ctgatctgag ccttcaaggt
    66061 acatttcaac ggggatgttg cccaattctt tttgtcgttt ttctacaacg gcgcgccatg
    66121 taatgccgga gtcaaaccaa acatccataa tgtctttttc ttttcggaaa ttttcgcctt
    66181 tgcaattagg acatttgaaa ttgttcggca acaactcttt ttcgctgtat tttacccacg
    66241 catcggagct ttctttgtcg aataattcgg caacgtgttt gattgtttca tctgttacaa
    66301 tgacttcacc gcaatctttg caatagaaaa caggaattgg aaccccccaa gctctttggc
    66361 gcgaaataca ccagtcggag cggccttcta ccatgttaaa aattctgttt tcgccgcttg
    66421 cgggaatcca ttttactttt ttaatttctt caagcgtttc gtttctgaat ttgtcgactt
    66481 ttacaaacca ttgaggagta gcgcggtaaa tcaccggttt tttgcatcgc cagcagtgag
    66541 gataagagtg gataatttct gattttgcaa gcagtgcacc tttttgttca agtctttcta
    66601 tcacaatggc gtttcctttg taataaggaa taccctctaa atccggcact tgatttgtcc
    66661 aaatgcctct gccgtcaagc ggtgaaaaga cttccaaatt gtatttaacc cctatctcat
    66721 aatcttctaa gccgtgtccg ggagcggtat gtaccgcgcc tgttccggca tctgttgtta
    66781 cgtgttcgcc caaaattata ggagatttgc gctcataaag cggatgaatt gtgtttaaaa
    66841 gctccaattc ttcgcccaaa acagagccga gtactttaat atctttttct tcccagccaa
    66901 cgccctgtaa aaagcttgcc aaaagtgcct gagcaattac atacacatca tttttgtaat
    66961 caaagaaagt gtattccaat ttagggtgaa gacaaattgc catattggaa ggaattgtcc
    67021 aaggagttgt ggtccagatt actgcgtata gtgagcggtg aacggtgaag agtgaaggga
    67081 taattttttg gattttattt atggattctt cgtcgaattt aaaccttaca taaattgatg
    67141 tagaagtgtg gtctgcgtat tcgacttctg cttctgcaag tgcggtttcg caagctgcac
    67201 accaataaac aggttttaag cccttttcaa catagccgtt tttgtacatt tgtccgaaaa
    67261 ctcttatttg ctctgcctca aaatcgggat caatagtcaa ataagggttt tcccagtcgc
    67321 ccaaaacacc cagtttttta aattcggctt cctgaccttt taagtttttg tgggcaaatt
    67381 ctctgcactt ttgtcttaat tcaaacggac tgagtgcttc tctgcctcct tttatgtttt
    67441 ttacaacggc attttcaata ggaagtccgt gcccgtcata accaggaaca taaggcgaat
    67501 aaaatcctgc ctgagtttta tattttatca aaatgtcttt taaaatttta tttagagccg
    67561 ttccgatgtg aattttgtct gagcttaaat aaggcgggcc gtcgtgaagg acaaatttat
    67621 gacttttatc ttttttttcg attattttgt cataaatatt ttcttcaacc cacttctttt
    67681 gaattaaagg ctctttttgc gccgcgtttg cgcgcatctc cagagtggtt tgcggtaaat
    67741 tcagtgtgtc tttgtattct tttcccattt ctaattcctt atatcgccat tatgcgggct
    67801 gcatcttttt gatttttgct tcccttacga actctctcat tttatcatag tcaaaatcgt
    67861 tttcccacct tgcaataact acggttgcaa cgcagtttcc gattaagttt acagttgttc
    67921 ttcccatgtc aagaatttgg tctatcccta gcaaaatggc aaccccgatt agaggaatgt
    67981 taaagcttgc aagagttcca gcaagcacaa tcagagaaac ccttggaacg gcagctattc
    68041 ctttgcttgt aagcataagc gcaagcatta tcataatctg ttgattaaat gacatgtgaa
    68101 ttccgacaat ctgtgtcgca aaaagtacac ccagtgccag ataaagcgtg cttccgtcga
    68161 ggttaaacgt gtatcctgtt ggcattacaa agcctacaat attttttggt acgccgaatt
    68221 tttccataat ctccattgct ttaggcaaag ccgcctccga gcttgcggta gaaaatgcaa
    68281 gcagggcagg ctcttggatt gctttgatta aatttctcaa agaaattctt gctatcttgc
    68341 aggcaataaa aagcaccatt gcaacaaaaa ttgcaagcgc aaagtaaagc gcaaaaataa
    68401 cttttgcata acccagcaga actcttactc cgttttcacc gactgttgaa gctatagcac
    68461 cgaaaatccc aacaggcgca aaatacatta catattcggt aaatttgaac ataatttcag
    68521 aaagcgaatt taaaacatct aaaacaggtt ttgctttttg tcccaccgca cacagagcaa
    68581 gtgcgaaaaa tattgaaaat actactattt gaagcaagtt gccttctgcc atggattgaa
    68641 ttacgcttgt gggaaatact tccgtaatta actcatgaaa agaactgtga acatgtactg
    68701 ccgccatttc ggttgcagtt ctcaggcttg aagggttaat tgtaacccca aacccttcgc
    68761 ccggcttgaa aaaattcccc acactcaatc ctatgataag cgcaagtgtt gttaccacct
    68821 caaagtaaac aagggtttta agccctattt ttcctatact tttaacatcg ccgtgtcctg
    68881 caacacctac aacaagagtc gcaaaaagca aaggtgctat tatcattttt accattcgca
    68941 ggaatattac cgccagaatg tgaagctttt gggcaaaatc gggaaaaagc cagccgacaa
    69001 aaacgcccaa taccaaagcc ataaatattg ccagggtgaa tttagatttc ctcaaaactt
    69061 ctttttcctt aaattaccgt aaaattaaga cgttgaatat attatataat aaaaatgccc
    69121 cgaaagttta taaaaggctt aaactttata aatttttaat aataggcact aaatatgata
    69181 atttttttgt cgttgcgagg ggtgaacaat atatttaccc cgaagcaatc caaaatgttt
    69241 attatttttt aagtaccttt tggtggcaaa aggtactgcc aaaagccacc ccctgctcgt
    69301 cgcgactatt agagtacggc tcattccatt gaaaatttta aactcgtttc gctaaagcta
    69361 cactcagaca ataaaatttt cgggcattgc atttcgccta ctctaataac gccgctcctc
    69421 aaaaaaaggg ggggtaattt tttattcgtt ttgataagtt caggatgacg caagaggc
//
LOCUS       DAOQAU010000003        64155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_310_length_64155_cov_7.890374, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000003 DAOQAU010000000
VERSION     DAOQAU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 64155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64155
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_310_length_64155_cov_7.890374"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1021
                     /locus_tag="PKI94_00870"
     CDS             323..1021
                     /locus_tag="PKI94_00870"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNW25323.1"
                     /translation="MKINAGVNFIENGLTASLRAMHVNSELVGMINENVIGFDKVGYQ
                     RKEPIISSFTEFLGVNGISATVDDKVGRLTATSSPLDLALAGKGYFQTATKNGVKLTR
                     DGRFKIDKFGNLLTTDDAKVLGNNGLAIKFPNPPESLKDVIVGSGGKISLFNKAKGKV
                     EYVGTLGVVDSNGAAVVTPDVKQGYTEFSNVSLEREFLSMMPIIRGFDANRQLFMIQS
                     QNLTKAISQLGSVS"
     gene            1040..1756
                     /locus_tag="PKI94_00875"
     CDS             1040..1756
                     /locus_tag="PKI94_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HNW25324.1"
                     /translation="MYNIQGIIRKSLNNTTTQFDRLGYVANNFANYNTNGYKSVRFEQ
                     MLQEDGYLKGAVRTDYEVGMMQSTGNPYDVAINGQGFIPVTSTSGEVAYTRDGSFKKG
                     KNGYLLTNDNWLVGEGIKIPPNCYKFAIKPNGDIVSYDSGGKSGEILGKIPLVQFANP
                     EGLTQGDGNKMKASDESGEAFLVKEHNYIAQGSIERANVNVYDSVSEMLRLNASMIAS
                     MRMAKVVDDMYNKAINIRQS"
     gene            1866..2216
                     /locus_tag="PKI94_00880"
     CDS             1866..2216
                     /locus_tag="PKI94_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HNW25325.1"
                     /translation="MTFTPIDSMGKASLMMDLIAQRQKAIGSNLANVDTPGYVRKDIN
                     FSQYLGSMDSPLETKLSGKLGPSAIVEDRNYEEINPANELMELQKNSLLYTMATRRMS
                     NIITQMRTVINVGK"
     gene            2219..2662
                     /locus_tag="PKI94_00885"
     CDS             2219..2662
                     /locus_tag="PKI94_00885"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNW25326.1"
                     /translation="MGILDSMNMGSKSLKVHSLRLDIAAKNIANVDTPNYVRKIPVLN
                     ATEDISFHGLMNEMKNDVFSTGAMPYVQGGVAFTGVVEDPTMGEKIYKPGHPDADKDG
                     YIRASNVNPMVDIADAMLAQRAYEANLALINITKAMASRATEIGK"
     gene            complement(2750..3352)
                     /locus_tag="PKI94_00890"
     CDS             complement(2750..3352)
                     /locus_tag="PKI94_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNW25327.1"
                     /translation="MISPQEALEKLIAGNERFAQNKSIHPNRFEETREVLLKQQKPFV
                     AVLSCSDSRVPIEIIFDAGLGDIFSVRTAGHVLSKEVLGSLEYAVKTLGVKLVIILGH
                     ENCGAIRAAIETQKDDSKISENLKAIMEHIYPAIESSECIEDSEDFLHCAVVSNIKYQ
                     LRDLLKKDEYLAQKVKTGEIMLKGALYNLATGKVELVNGK"
     gene            complement(3375..3779)
                     /locus_tag="PKI94_00895"
     CDS             complement(3375..3779)
                     /locus_tag="PKI94_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25328.1"
                     /translation="MDVKKKILIVDDSRGWIEYHKQTIQKLFGAQFALEEANCASGGY
                     DMVYNNLNIPYALIISDLQMELDFEPKYAGEWLIEQVKNLKEYQSVPIIIISATYDIR
                     NIASQLGVNCLPKSIAARDLMSYKLAIEECLR"
     gene            3931..4323
                     /locus_tag="PKI94_00900"
     CDS             3931..4323
                     /locus_tag="PKI94_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25329.1"
                     /translation="MDIRPVSLNFGNSKITFQKALPAFKLNNLDLLDITKKVQEYKVV
                     NQNNEVIIHELNSAGKRILKCLYKRITDTLSPDYGKLWLREKTAYSHIDGKISRKILY
                     NKYGEVVEAQAYNGNARPYTYHLNPDFV"
     gene            complement(4304..5413)
                     /locus_tag="PKI94_00905"
     CDS             complement(4304..5413)
                     /locus_tag="PKI94_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW25330.1"
                     /translation="MFRRILSLIKKEFIAIWADPRSRILIIVPPLLQLFIFAHAATME
                     VRNLHVAVLDKSNTLQSRELTADFANSRWFKKIIYVKDENELAKLIRQQKVQMGLSFN
                     TDFATKLLAQKTADVQVIVDGRQTNVAAITNGYASEIIAQYEAKSFPKRYKSVPRINL
                     EVRNWYNPNLIFLWYTVISLISILATTIALVLTALSIARERELGTFDQLIVSPLSSLE
                     ILIGKTVPPLIISIVLSSIMTLAATFAFQIPFTGSIWLFWMGMFFYLLSILGVGLFIS
                     SICKTQQQAILGAFTFQMPAILLSGFISPVEEMPIFFQYFTYVNPIRFFLVIMKGLFL
                     KAMPLHDVAQNIIPLIFIAVLTLCVATWTFKRNLD"
     gene            complement(5416..6528)
                     /locus_tag="PKI94_00910"
     CDS             complement(5416..6528)
                     /locus_tag="PKI94_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW25331.1"
                     /translation="MQILKALIKKEFNQIFRDPSSILIAFVLPLILLIIYRYGVNLDT
                     VKIHMGIKIDDTSQQVVTLIDSFKGSKYVQPIFYQDKNKMYDDLTGSHIRGIVVIPND
                     FSTKLDRGEIASAQVITDGSEVNLANYAQNYTKSILQQWLTYSSKYSKKIPPPLINVQ
                     TRFWYNQKIDSHYFVLPGSLSVTMTLIGMLLTALVIAREWERGTMEALLATRVRKIDI
                     VLAKYISYFILGMSSMSFCVFLCISVFQIPFKGSFLVLFLFSGLFLLTAMGQGLLIST
                     LLKDQYNASQAALVAGFLPALMLSGLVYPINSMPLPLQILSVFMPARYMVSSIQNEFM
                     AGTVWGIILPNCLFLATLSVILFAVVYKKTATRLED"
     gene            complement(6722..8233)
                     /locus_tag="PKI94_00915"
     CDS             complement(6722..8233)
                     /locus_tag="PKI94_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW25332.1"
                     /translation="MISSIEIKNLTKNFSDNCAAIDNLSLNIEKGKITGLIGPDGAGK
                     TTLIRLIAGLLVPTEGSISTLNLNPETQKELINRKMGYMPQKFGLYEDLSVIENLTLY
                     ADLKGIEKKDRAEIFEKLLTFTDLKNFQPRLAGALSGGMKQKLGLACALLGTPEFLLL
                     DEPSVGVDPISRRELMQMVKELAGGGITILWSTAYLDEAHSFDNCIVLNDGKIIYNGK
                     PQELSSTSQGFEDEVIKLMGGYQKKESKLAKSYDLREQDGEFVIEALDLVKKYGNFYA
                     VKNNTFHIKKGEIFGLLGPNGAGKSTSFKMMCGLSTPTKGTAKIMGQDILKNPSKARS
                     HLGYMAQKFSLYGGLNVEQNLRFFSGIYGLGLKERKEKIDEIIDIFHFEKYLKSKASE
                     LPLGYKQRLSLACAVMHSPPVLFLDEPTSGVDPISRKEFWNHITSLAQKGVTIMVTTH
                     FMDEAEYCDRISLFYRGETIALGTPTELKNMAGAKNMEEAFVTLIERSEEVNR"
     gene            complement(8356..9198)
                     /locus_tag="PKI94_00920"
     CDS             complement(8356..9198)
                     /locus_tag="PKI94_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW25333.1"
                     /translation="MMKKIIILVFLISLSGLIFYSISSKKVDKDFLTLYGNVEIRQVD
                     LGFRVEGKIAKMLVEEGDAVKKGQLLALLEDENYKATYEKSLADIELYRALSTNAASK
                     YERNIPLCKDSTTSKQECDDLLNSKNSTKASFEAATAASKGAKKNFDDTRIYAPDDGI
                     IMTRIQEPGASVTPSQPIYTMAKNKPVWVRAYIPEPNLANVKYGMEAKVLTDTKNPQT
                     GKQREYKGQVGYISPVAEFTPKTVQTTDLRTDLVYRIRVYIYDLDEYLRQGMPVTVKI
                     KLNS"
     gene            complement(9195..10559)
                     /locus_tag="PKI94_00925"
     CDS             complement(9195..10559)
                     /locus_tag="PKI94_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW25334.1"
                     /translation="MQKIKKIFALFLISIFFIQIPCFAFWGKKFCPCPINTKEKIEYI
                     NPCWWENFNDPILTEYIIKAVENNHNARQASWKVEEYRQAVKYQFGSELPSLSVGGNY
                     ILNHLPDSFVSTKNNIFAVPFQVNYEADIFLKNRDKTKSSKKTYESSKYEEKAIYIVL
                     ASDVATAYFNLVKSDKQICLQEQIVEIRREKLRREQQKFDRGTVSATSLNNAKKEVEN
                     AKNSLDDFLKTREKTLTQLAVLIGESPENTNFKRVSFDNLEYKCPVPDSISSDVVFSR
                     PDIMATEAKLQKAKIDIRVARKEFLPKINLTGIYAFSNVGSLGFGTWGSTIAALVAGA
                     SLDLFTGGRKIANLKMSKSRYEQMFEAYRQNDLTALKEVNDSLFVLKKDSEIDKNTLK
                     NLNIQQDTYKRSLERFNRGTISYPQLLSEHEALILSQQNRVNSKTNRLVDYLTLYKAV
                     GGNL"
     gene            complement(10561..11229)
                     /locus_tag="PKI94_00930"
     CDS             complement(10561..11229)
                     /locus_tag="PKI94_00930"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HNW25335.1"
                     /translation="MSFKYGGFMPKKPDSSQKTKRKILDSATELFAKKGFDGISTRDI
                     CKAAGVNISMISYYFGGKEELYSKVVSSIVENIIQYVMNNMGTKELPQSFEHLSKEEK
                     IAALFKFLDMMMDYFYSDKISDSEIMIIFREQMTSGVPLNAEGYKVLKRLLASILGRD
                     ENDKEIIFRTITIVGQIHSAKILKQFSLKLMNQSGYSKQDIQMLKQIVMGQVKAILKD
                     LGAV"
     gene            complement(11499..11581)
                     /locus_tag="PKI94_00935"
     tRNA            complement(11499..11581)
                     /locus_tag="PKI94_00935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11546..11548),aa:Leu,seq:cag)
     gene            11715..12506
                     /gene="surE"
                     /locus_tag="PKI94_00940"
     CDS             11715..12506
                     /gene="surE"
                     /locus_tag="PKI94_00940"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNW25336.1"
                     /translation="MKILISNDDGIVSNGIKVLSETLAEEHEVYIVAPDRERSAAGHS
                     LTLHTPLRVEELEAKNGIKRCWVTTGTPGDCVKIGLSAILEENEKPDLVISGINHGPN
                     LGADILYSGTVSCAMEGAMLGYPSIAISLASMRCELEDFKFPAKFIASLLPKLKEFDF
                     PKKTILNVNVPALDPEDISGVAITELGKKMFTDNYEKRVDPRGKIYYWLAGELMNEPE
                     DAITDIAAVRNNKISISPVTYEMTRKDSMKELNKILCSGDACEWY"
     gene            complement(12563..13408)
                     /locus_tag="PKI94_00945"
     CDS             complement(12563..13408)
                     /locus_tag="PKI94_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNW25337.1"
                     /translation="MNIIQAILMGIVQGLSEFLPVSSSAHLVFTSSLYKYFTGVLNVI
                     QSNEEVFFDIMLHLGTLIAVLIFFRKEIVQIIKSLFNAIKTKDFSDYEAKLGLYIILG
                     TFFTIFVAYPLKDFCEKLVFSPAIVGVLLFITGIVLLLSEFVSKKIPQKTDKINLKNS
                     ILIGIAQGLAAFPGFSRSGWTIATGLFSGLDRVTCAKYSFLLSIPIILGTSMLYPLLE
                     IDFKEIAAFNWTSIISGTIVSAVVGYFCIKYFLKFLQKFSLAFFAYYCMIVGAIMAVF
                     FKFIT"
     gene            complement(13475..13834)
                     /gene="queD"
                     /locus_tag="PKI94_00950"
     CDS             complement(13475..13834)
                     /gene="queD"
                     /locus_tag="PKI94_00950"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNW25338.1"
                     /translation="MFELKIQMYFSAAHHLLNYEGDCENQHGHNWLVEVFVQGNSLDK
                     SNILIDYKILKKELKAVLDILDHQDINNLPEFKGISPSSEIIAKFIYEKMKEKIKIVS
                     KVSVWETPTSCASYYVN"
     gene            complement(13822..14364)
                     /locus_tag="PKI94_00955"
     CDS             complement(13822..14364)
                     /locus_tag="PKI94_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNW25339.1"
                     /translation="MIKDIQLKSIAKKIKLVAFDVDGVMTDGSLTFDENGVEYKTYNS
                     KDGQGIVMLIRAGVIPAIITARNNGTVVHRAKILGITELYQGQKNKIHALDEIIEKYK
                     ISYDEVAYMGDDIPDLCVLEKVGLSCCPADAVEEVISVCKFVSTKGGGKGAVRELADF
                     ILKSKSVNLKEKGGLSACSN"
     gene            complement(14490..15506)
                     /locus_tag="PKI94_00960"
     CDS             complement(14490..15506)
                     /locus_tag="PKI94_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNW25340.1"
                     /translation="MIELNHNPPKTGEKMQMTKSINDLAKEVLDIEANSILKLKDKMN
                     GNFEKAIEILYACKGRVIVTGMGKSGLVGKKIAATLSSTGTPSYFLHPAESTHGDSGI
                     ITREDVIIAISNSGQTQELLNLLPLLKRFDVPIIAMTGVLNSTIAKSSDVVLDVSVEK
                     EACPLNKAPTASTTATLAMGDALAVCLLEKRGFSEEDFLIYHPSGNLGKGFLYKVEEL
                     MLSGEKLPLASQDDKFTDVIELISNKKLGLAILTDEKGIMTGVLTDGDIRRTLIKYKS
                     VENLKAKEVMTVNPKTVLPDALAAKALNLMEKYSITALAISDKKGFPVGVIHIHDLLK
                     AGVA"
     gene            15647..15943
                     /locus_tag="PKI94_00965"
     CDS             15647..15943
                     /locus_tag="PKI94_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25341.1"
                     /translation="MTQNLLERNFINPPAHEACGALDIVESMKKYIFENDCPVMSMDV
                     SHLNVIDASKVAILCSTYHYAKYPHGHIDWFVNSSEIKNLVKPLNLGNIDLITV"
     gene            complement(15948..16646)
                     /locus_tag="PKI94_00970"
     CDS             complement(15948..16646)
                     /locus_tag="PKI94_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNW25342.1"
                     /translation="MKKFLLILGLLLAGLTVKADFVESSSGVTMENFWEKNGKEQQKI
                     LEVGTRIINANQLDKRIPINLDKTPKVINAHASLTNKTITVSSGILPYIDNDDELAFI
                     MSHETAHSLDAYKGGFEAVMMKLNTREYEYRADLKGIDLMTKAGYNPIAAICVSNKFM
                     PEESWDFFTTHPVTSKRLFAMYKHIYKNYPWALKTDMTQNINFKNFSRTAETQIYYFQ
                     QAEKNFTKKPENNL"
     gene            16795..17676
                     /locus_tag="PKI94_00975"
     CDS             16795..17676
                     /locus_tag="PKI94_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25343.1"
                     /translation="MNNLCVDNFSPVSKVNFCGTTIPQYKPLKPLSQDTFERTTSKKE
                     ISFEGWPKIFSEVGAVTTEAVAKLSKAEMQHLTGVAKSKIDEQLVDDITRFSQILKKG
                     LDAKFNKKYVFVSIGQSPAAFADVLNATGVETAICPISNLSNLKNASPLLRNPNLSKY
                     FEYMKKIGLDPNKMKNSGKQYIFVDISSTGKSLKIFEEFLRDKKSGFNLPNVKFVSLQ
                     DLMSSCKPSNSEEASFMEEFFEEYIKTPELKKEFSSIFKLDYKDIDKVQQYRDKTPAN
                     EKLNMLKLLVLNKVLGR"
     gene            17918..19762
                     /gene="glmS"
                     /locus_tag="PKI94_00980"
     CDS             17918..19762
                     /gene="glmS"
                     /locus_tag="PKI94_00980"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNW25344.1"
                     /translation="MCGIVGYIGDKPVVDILVNGLTSLEYRGYDSSGVALHADGKIKI
                     YKAEGKLQNLKDVLAKVTNETKTAKMGIGHIRWATHGAPNEINAHPHTCTCGKLVLVH
                     NGIIENFKELREELIKKGCKFCSQTDTETVAHLVAQEFHKTKDLTKAVNQATKRLHGA
                     YALVIMHQDLPGMIVATKRNAPLLVGLGEGEFFVASDVPAIIEHTNKALYLNDDEIVT
                     ITKEKMVLSDSQGNELSQKIEVLPWEPVALSKMGFKHFMLKEIHEQPDVIRNVLSGKL
                     YNIDEPISLKEVQLSKETLKNLNRIQIIACGTSLHAAMVGKYLIEDFCNIAVDVEPSS
                     EYIYRKTVTDSKTLVIGVSQSGETADTITAIKQSKEKGSHILIITNRPDSTMAREADS
                     LVPINAGIEVSVAATKSYTAQLISFYLLALYMAEIKGTLDNDFLTELKRELIVLPQKV
                     ETLLSHKEEIQKCAKAFSATKDFIYIARGINFPTALEGALKLKEISYINATGYPAGEL
                     KHGPIAMLDETMPVLSILMQGKVYEKILSNAEEAKARNARLIALTNSKDEKLGELFDF
                     ILRVPEVNEIFSPVIAMIPLQLLAYYIAEFLGKDVDQPRNLAKSVTVE"
     gene            19759..20034
                     /locus_tag="PKI94_00985"
     CDS             19759..20034
                     /locus_tag="PKI94_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25345.1"
                     /translation="MTQINSKLIEIEVPEVFDNDFIEQKLSEMGIEPLRWAVVSGSRV
                     WKLEGRALPACRAVTDRWRRKRLHCKRHRSNLANKIILTVSAAFESL"
     gene            20130..>21394
                     /gene="ahcY"
                     /locus_tag="PKI94_00990"
     CDS             20130..>21394
                     /gene="ahcY"
                     /locus_tag="PKI94_00990"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNW25346.1"
                     /translation="MLMTQQKYDIKDINLADQGKNQIEWAFKDMPVLNQIQERFIKEQ
                     PFKGLKLSACVHVTKETAALCVVMKAGGADAILVASNPLSTQDDVAAALVKHYGIPTF
                     AVAGESVETYKEHIKEALNHNPDIIIDDGCDIVAMIHAEHPEVAQKVIGSTEETTTGI
                     QRLQALEAEGKLKFPAVGVNTSMTKHLYDNRYGTGQSTMDGIMRAANILIAGKTVVVS
                     GYGWCGKGCAMRAKAMGANVIVCEIDPLKALEAAMEGYRVMPIDEASKIGDIFITVTG
                     NKHVVDVKHILNMKHGAFVCNAGHFNYEVNVPDLANETVEIKKIRPTLEEYKLKNGKS
                     VYVLAEGRLVNLISAEGHPASVMDMSFANQALGIEFLVKNKGKLENKLYTLPVEVDNE
                     IAKIKLESMGISIDTLTPLQQEYLRSWNI"
     assembly_gap    21395..21494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21495..21782)
                     /locus_tag="PKI94_00995"
     CDS             complement(21495..21782)
                     /locus_tag="PKI94_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25347.1"
                     /translation="MSKELVKQICHIAKKYKVETVAIMTNHLKIKGKLANCPPDDESD
                     CILTLNDAKTWLLEDLCKCSDQKCGCESHFVSHSEWLGINADKIVSFSIYK"
     gene            22015..22353
                     /locus_tag="PKI94_01000"
     CDS             22015..22353
                     /locus_tag="PKI94_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25348.1"
                     /translation="MNVNTLNGLSFQAKTQNGNEYKKTGFFRTALPVLSSAAVIGGTY
                     INKDIDATFARKSFMEEIKKSAKKPKNILVFAAVLLGAGVINFIIGAGFDSLINKHRS
                     NKADKKAVQA"
     gene            22729..23364
                     /locus_tag="PKI94_01005"
     CDS             22729..23364
                     /locus_tag="PKI94_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HNW25349.1"
                     /translation="MAESDEQNLVYLLDGKIYINLTNKCTANCVFCIRSLKDDVCGAN
                     LRLKNENFGAQEVIDQLKKLVKNEREIIFCGYGEPTLKLDILKEVANFLKTSYNNVKL
                     RINTNGHANCVYKRNIIPELKGLIDEISVSLNASNKEQYEELTQPKLPCENPFEEVKD
                     FIKKSVEEGIETTVTVVNGYKNHKIDLNACEKIAQDLGANFRVRQWLDEGY"
     gene            23539..25704
                     /locus_tag="PKI94_01010"
     CDS             23539..25704
                     /locus_tag="PKI94_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNW25350.1"
                     /translation="MKQLDKIMKPKSVAVIGASTKPLAIGSEIMLRLKEYGFKGDIYP
                     INPKADQIEGFKAYPSVLDVPGEIDFAVVVIPKQFILDTLDQCNQKGIKGLCIISSGF
                     KEVGSEGAELEKQVLAKAQEYGMRFVGPNCLGVLNTNDNVRLDATFAEALPEKGGVAF
                     VSQSGALGGGILNILKDLNLGFSQFVSIGNQADINAETMMEYWEDDEDVKQILLYMES
                     IANPANFRKLATRISKKKPIIALKSGRSAAGASAASSHTGSLAGADKAANALLAQSGV
                     IREYSLKNFFDTAKVFSNCPLPKGNRVAIITNSGGPGIMATDAVCEHGLEIAKIKEET
                     KDYLRSFLPDVASVKNPIDMIASAPAEHYQKTLTAVIEDENVDMIMVIFLPLIGQKDI
                     DAAKAIMEIKEKYPQKPIIGVFITKQEFFAKVADLNPNMPFFMYAEEAADGLYRLDQY
                     RRWLERKEGKMPCFEVDKAKAEKIIAQSISEGREQLTTLESIDVLDAYGIRACKYGLA
                     TNEEQVVNLGNEIGYPVVMKMTSKTTSHKTDVGGVRVNIQSEDQLRAEYQDLMAKLKE
                     KGLLEGLEGVIIQEMVKGNREMVCGIATDPQYGPMLMFGLGGVFIEVMKDVTFRIAPL
                     TDVDASEMVRSVKAFHLLEGARGTKPAQIEQIEETLLRLSQLVNDFKFIDELDINPLL
                     ISEKTGEGIAVDGRIKVRMAEAKEAINCNCSCGCGCSCK"
     gene            25814..27256
                     /gene="gltX"
                     /locus_tag="PKI94_01015"
     CDS             25814..27256
                     /gene="gltX"
                     /locus_tag="PKI94_01015"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNW25351.1"
                     /translation="MEQIRVRIAPSPSGNLHIGTARTALFNYLFAKKHNGKFVLRIED
                     TDLERSSDEYIKNIFDSLKALGLQWDEGPDVGGEYGPYSQSQRFETYQKYAQILIDKG
                     YAYECFCTPEELEEEKRISQEKKIAHIYSGKCKNLTDAQKKELRKEGRKPAIRFSVEA
                     RELIFNDLVKGELKLDTGLIGDFVIMKSNGSPTYNFAVVIDDMLMKISHIIRGEDHIS
                     NTPKQILIYEALGAKVPQFGHLGMILAPDRSKLSKRHGATAVSEFIEKGYLTEALVNF
                     VALLGWSPSDGVEIKTVDEIAADFRIHEVSSSNSIFEYDKLNWMNSQYIKKLGVNELK
                     EKLLPFLKDYDLSELSEEKLTRMIEITREPLVILSDITDAVSYLFGQGVNVEPEVQSE
                     VLDTEISQTVLKEVAQLAQNWNFDDEEALHHTLEELRNSFKERGIKPKETMWAIRAAV
                     TGRTKGADICAVLWSLGREKVLNRINKALK"
     gene            complement(27260..27922)
                     /locus_tag="PKI94_01020"
     CDS             complement(27260..27922)
                     /locus_tag="PKI94_01020"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HNW25352.1"
                     /translation="MTGVRKVQNLSRKKIINSAIILFASKGFDSTSTREICKHAGVNL
                     SLISYYFKTKEGLYISIIESVLNYGLKFIQEEIEKSNEIGKMTREEKIKLLFELLDKY
                     ISFIYSESVPNNFVILMIKEQTSSNSKFSNLYNEKIQIFYKAFRKILASILGRTENDK
                     TIIFQTSAIVAEILSFKFMTRAILTPLKQSAYTKEDLKLVRKIISENVKMSLEKLGIK
                     PL"
     gene            28245..30689
                     /locus_tag="PKI94_01025"
     CDS             28245..30689
                     /locus_tag="PKI94_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW25353.1"
                     /translation="MKRLLTFLLLGVFCTSMSLPALAAPVALSGAAAANVKAIKYKPN
                     ARSINYAFVFDGPSDKNEVVLKQFEKAITATTAPDYKAEFPKNLVFVGNWAQSGVKTA
                     SDKALASNATMVVSLGYLSSNYLADKKGKNKFVVTIDQYGLRDIGEGFFNPVQQSVNG
                     IKNFKTLLSFKKAAILMNENYYKTQKDWAKFAGDKIPNINFAVIPATTDVAATMASIP
                     SDCDAVVLTPLFNLSVEQRKNLISQLNAKKIPTYSTLGKEDVEMGVLLGTGAYDLDRK
                     VAEMTSFNIKGVLDGQTKMAGKVNFYEDEVLYMNRDTADLIGYQPHLRVLYNAEVISN
                     KKPTVYDLSTIFNLLETQNLDIQRKKLLVKAARRATAAAALRYLPSAVVNLGFQQYDE
                     DYAYTVKLMQPEKTGIFQIGLEQVLYSPALVTNIIIKKKGLNFAKHEQFMVEQNMGID
                     IALLYIETNMLENMIKVQKEYVKEARENLAIARVREKMGVCGNEETMRWAAQVNIKEQ
                     HLLDMREELKNLKIEINKMLFKDQKEQFELAALTAKDPAFYTKDLHVINYVTTPEKLE
                     KFTQMLIGEAFRVAPELAKLKTAIEMKNAEAAMYYQKFILPDAKLTYTYTSLMDRQFA
                     AGLPSAPFAYSPPAGGTIPGSMWTNLSALRPGATNSQLGIFAQWRPIEGGTKIAEIAR
                     INAERQELQRYQDEVKTAIEEQIRQTINRAVAAYFSIEKNYKAMYASQENYAKVKERY
                     LTGKAPIDQLIDAETTYLNSKEKAMNSQYVFFKELVWVQRAICAVNWATASTEAKQFI
                     ESIKKEITPQPDVSLL"
     gene            complement(30764..31789)
                     /locus_tag="PKI94_01030"
     CDS             complement(30764..31789)
                     /locus_tag="PKI94_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNW25354.1"
                     /translation="MNNLQKFQNNQIVDKDETQYLYFSLGKKKYAVEVSQVVEIMKLP
                     YLEYPQKLKNNVIGLLGYNNFTLNVLDLRFYLNVKVTPYSLSNQLLVVKTDESMFGLI
                     ADKVEDIVSIENSSLEYNSQIKKEKIVDFSYRNKGEIISVINLNALENILKKGVKDEN
                     INISSLFPNDDDSRYKLMQRNTELSEKSKQGLVKNIFSQDEFISFLLGQNVYCISLEY
                     VKEFLKDVSITTVPCTPDYIAGIITLRGDFITVVDVKNFLGIPYKKSLQKSNGVIILE
                     TVDFKIGFLTDEIFDIMNIAEELIIQNSYNRMNKYILSEVVIDDKLYKILNMKNILSD
                     ERFFIEE"
     gene            complement(31761..33248)
                     /locus_tag="PKI94_01035"
     CDS             complement(31761..33248)
                     /locus_tag="PKI94_01035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNW25355.1"
                     /translation="MANKILLIDDSTTQLESLKIQFTKAGFEVKTAQDGADGYKKVFE
                     NAPDIILSDVIMPNLNGYQLCRLLKNTPATKKIPIVLFTVLDKKIDEFWAGKSGADAF
                     MSKNENFEDLHIKVCELLEQKRVSEDYKKFILNQQIREESVQNQINDVLDELLMNSTF
                     LNEFRDLGEYLTHEKVLIEKTFELLSMFIDYNVAGIFFKSLDKNDKNILHLDINKSFV
                     SNFVIEKIKRDFFSNMPDMPEFTIRDFGHDIVREKINEEEKIVSFDDIKSSHVFPIIF
                     EDKLLGGVCFFSNNQINYSDFKFYNTMVSEFLLLFKMRFLYSETEYLSVTDGLTGLYN
                     RRHFEYNLEREFSRVMRYKGDLSLAIIDIDFFKKVNDTYGHQYGDYVLREISDILSAS
                     FRKTDMIYRYGGEEMAIILTETTIENALIPIERLREKISQYKFCYNDIETNITVSIGL
                     STNFETIETEKDFVESADKALYKAKQDGRNRVVIYSYEQFAEISK"
     gene            33361..34413
                     /locus_tag="PKI94_01040"
     CDS             33361..34413
                     /locus_tag="PKI94_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW25356.1"
                     /translation="MKTDSKFNLGQRIKIKNIIDISLLLLMITAVLLSGFSMKIGRYD
                     ALIMSLVIIFYIAQYIMSQNWINNAFKISVKKQINQTTAATEEIFDSVKKQKNSLLEH
                     LESVKTALENIKELKANFEKAKQSSSNVSSKAEKSLDFSQKEQEAIKANIEKMITLRQ
                     KIQIIAELILELSEHTQQIGSIIGIVEDIAEQTNMLALNAAVEAARAGEHGKGFAVVA
                     GEIRKLADESKQATTKITNLIKDIQQATNSTVMATEEGSKEIELGVKLADNINQNVDL
                     LTGLIKEVNLSAEEICSNSKIQTNYSEKAQEAIENVCEGIKTSLDSLEENIQKIQALN
                     FISVSFKENFTDEQDF"
     gene            34414..37515
                     /locus_tag="PKI94_01045"
     CDS             34414..37515
                     /locus_tag="PKI94_01045"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNW25357.1"
                     /translation="MDFQEEEFNEILRIFQIESEEIISRLNNNILELEKTPDNKDIIV
                     ILFRDAHSLKGAARMVGFNHIQTLAHKIEDILGLAKDNQIRLSPETVNALYKSVDFLS
                     EIIGKSIEQKTEFYDENLAVQISLLDKISAQPQENTEKSKEEFSIKELKNNINEINSL
                     IPEGLFELMSALSGQNQEIIKEFSQKIDRLFEIFKQVGIYDIKKSLEDIKVKLDFVIK
                     TSGSFNLKEIDDFQKIIDGVISNLVSVCEIHAIKTPDYYSLAFEMFSAKSDRSSAQKE
                     PDMPEKTLEHQEEIFDLPPEAIVQKQEVKEFDNKNLLNIKEMFSSLGQNYTSLGELKQ
                     KLLDFETTAKDEIISPIINNTLKIIDFSIKNKMKLNEDAISAMEQGIDYCLSRLNNQE
                     EATDSALVIQRLEIIQQVLELSNQSTENENILPRKSTDLIKKNSDLNAIFDTGEIKTL
                     RVDSIKLDSMVSQVGELMIAKIKAKKHIYGLDSINISMDEWHKDCAKVLNHLKYFEKK
                     YPKLQNEDSQSFYFINQILNVLENNCRKFSDTLKEISNLRRMIQEDETKISLIIDELE
                     STVKNIRVLPFATIFHIFGRMVRDIAQEKGKQINFEISGSETSTDKKILEEIKAPLIH
                     IIRNSIDHGIETPQQRISAGKDPVGKIILKASQTDNKVKIEIHDDGKGIDVAKIKEKA
                     LKKGYLKKEEADSMSDEQITNLIFAPGFSTGDEITSISGRGIGLDVVQTKISQLNGKV
                     KVLSELNKGCSVIIELPTTMSTLKSFLVRASGQIFAIPMSVINSVMLKNSNEIFFNEG
                     LKTIIIEGKTVPIYNLSAILNLPLHEQNSNKKTILTLESDNKIIGLEVDKLLGDQEIL
                     QKKLCAPLYKLKNISGVTTLASGDLCFILNISKILKNINKINYIEQSQPYEKISVKEN
                     KILLVDDSITTRTLEKNILTKAGYSVELALNPIEALEKLSKEKFNLIISDIEMPEMNG
                     FEFLRKIKSDKNYSDIPVLMLSSLYNEQERKKAKDYGAKEYLNKNEFNQEEFLGIINN
                     IFQ"
     gene            complement(37520..40279)
                     /gene="mutS"
                     /locus_tag="PKI94_01050"
     CDS             complement(37520..40279)
                     /gene="mutS"
                     /locus_tag="PKI94_01050"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNW25358.1"
                     /translation="MSKQEINHVNSLDINIEDVTPVIAQYLEIKRQHQDVILLYRLGD
                     FYETFFEDAIIMSRELELTLTGKDAGAKMGRIPLAGVPAKAVENYLEKLIEKNYKVAI
                     CEQLEDPKETKTLVKRGVVRLVTAGTLTESNLLDKNSNNYICAIIKDKRDDNLFGFAY
                     ADISTGEFKTTHAPLSMILSELARLQPSEIIAPSIAQKVMPFQIVPDEKIDLPDEIVN
                     NYNCSKVPSSIFEQNFALNNLKTVFNTNSLEAFGYENYKTGFLACGALVSYIWETMKE
                     NFPKFDTITPYELSEFVVIDANTRKNLELTQTLRGQNKYGSLLWAIDKTKTNMGARLL
                     KNRICQPLKNVDEINKRLDIIEELIQKPQTRGNLAQILEKIYDIQRLSTRLSNNSANP
                     RDFLGLKDSLFMLPQIIKEIQGLESDSFDKLSQYEQELIEFANIIERTIREDAPISVK
                     DGGVIKSGVSGELDYFRDLLTGGEKWLKEFEEKEKERTGMKFLKVGYNKVFGFFIEIT
                     NSNLNLVPNNYIRKQTLTNAERFITDELKKHEDDVLSAQFKALELENKLFCDFKNYAK
                     EYVTKIRQVADCIANIDVLTSLAFVAVENNYTRPTVDESRDFLVKNGRHAVLEKVLPL
                     GSYVANDLELAYQKETVNGKQETDLSLREVSEANDEATQKYPSPEFQALEQSSRAEIL
                     PSPSRGEGLSSHSSTQFMILTGPNMAGKSTFMRQNALIAILAQMGSFVPADYVKLGVV
                     DKIFTRVGASDDLTLGQSTFMVEMIETGYILNSASEKSLILLDEIGRGTSTYDGVAIA
                     WAVAEFIATKIKARCIFATHYHELNVMTNTYPQIKNYRITISEENGEIEFLRKIVQGG
                     ASKSYGIQVAKMAGLPQSVINRSQDLMKKMQRDFSNDLSARKRSSKDEQNQLQIKAPQ
                     LNLFK"
     gene            40451..41053
                     /locus_tag="PKI94_01055"
     CDS             40451..41053
                     /locus_tag="PKI94_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25359.1"
                     /translation="MGCLKNIFKAIILVLAAIGFVSIGGKDYVINLWNQYTNPPQDVM
                     LERAKKVGDFSQVPDEYEIDKAASVLGYNGVLAEHRATGQKMVVVDSGKKPLLTPQDF
                     EDGSADKKLEDLTKKLKYQFINIQDLKITKHGKINSFGQKTNFIKFQAKANKLPIGEI
                     EGIISAVETEDGKIKIIASANEKGKYSQIVSEEFFKKVKE"
     gene            41217..41942
                     /gene="pyrH"
                     /locus_tag="PKI94_01060"
     CDS             41217..41942
                     /gene="pyrH"
                     /locus_tag="PKI94_01060"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNW25360.1"
                     /translation="MGKDLKYKRVLLKISGEALLGSQEFGIDPVPVEMIANEIKLAHD
                     EGAQIGVVVGGGNIFRGVKNSAKLGMDQASGDYVGMLATVMNALTLQSALKKVGVSCR
                     VQSAIQMNQIAEPYIRFKAIRHLEKNRVVIFAAGTGNPFFTTDTAASLRASEINADAM
                     LMAKNGVDGVYSDDPRVNKKAKKFDKISYDDIIKNGLKVMDTASCALCKQNNIPIIVF
                     DFQAKGSIKKILHGENLGTYVGG"
     gene            41944..42510
                     /gene="frr"
                     /locus_tag="PKI94_01065"
     CDS             41944..42510
                     /gene="frr"
                     /locus_tag="PKI94_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNW25361.1"
                     /translation="MSEAVDEMFLFGEEKMEKALNQLKKEFASIRSGRANPMILDKVM
                     VDYYGAPTPLRQMSQVTVQDGTTIVVSPYDKSIIKEIEKAIIKAELGIPPNSDGIVIR
                     LAFPPLTEERRKEITKDVKKLGEDSKVVIRNIRRDMVDELKKLEKSENLPEDVAKDNQ
                     DEIQKLTDKYVKMIDMTVEEKEKEVLTV"
     gene            42510..43418
                     /locus_tag="PKI94_01070"
     CDS             42510..43418
                     /locus_tag="PKI94_01070"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNW25362.1"
                     /translation="MNLTLNKNAKRIITGSIMGLIALWCLIYGGIALLALITIVVFLG
                     SKEYVLILENKGFFPSLKVILFSDAVFALLCYFNRFDLVAIAFTICSITAFLWVLFKG
                     RQPYIANVSTTILGFVYGGWFPLHLLLVRGLNAENYHGFLKLNIQNDGLGYVMFLFTV
                     ILFTDTGCYYFGKKFGKHKLAAVISPNKTIEGAVGGTICAVISAFIVGHYIHLAWYHC
                     LVMGLLCTTFAQLGDLSESLIKRDAGVKDSGNTLPGHGGFLDRTDSYVLTIPVMFYYL
                     KYFVVSNQLWFDFVNFIKGFLHALGV"
     gene            43432..44118
                     /gene="rnc"
                     /locus_tag="PKI94_01075"
     CDS             43432..44118
                     /gene="rnc"
                     /locus_tag="PKI94_01075"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNW25363.1"
                     /translation="MEKIFKIKLENLKIFEKALTHSSFTKENDISSLENFEQLEFLGD
                     AVLKLCVSDILYKKFPKYSEGELTKIRSILVSDNTLAKIAKKIKLDKLLILGKREEKS
                     GGRQKNSILACAFEAVLGAYFLEGKYIDLSKNLEKILKPFIKEIDENFEKFNAKAILQ
                     EYTQSQNKQTPLYKIVEEKGPQHDKVFVIEVSYMEKVLAKGEGKTKKEAEQNAAMLAC
                     KKLGVINSEQ"
     gene            44197..44835
                     /gene="rpe"
                     /locus_tag="PKI94_01080"
     CDS             44197..44835
                     /gene="rpe"
                     /locus_tag="PKI94_01080"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNW25364.1"
                     /translation="MKTIISPSILSANFANLEHDIKAVEKAGAHWLHIDVMDGHFVPN
                     ITIGVPVVKSIKKITSLPLDVHLMIENPKKFVKSFVEAGADIITFHYEACEDKILEVI
                     YYIKSFGIKAGLSIKPKTDAKEIIPFLSDLDMVLVMTVEPGFGGQEFMHDCAQKIPLI
                     KQNSPDNMIIQVDGGINNVTGKICTSLGANSLVAGNYIFKSKDIEQAVKSLM"
     gene            complement(44898..45287)
                     /locus_tag="PKI94_01085"
     CDS             complement(44898..45287)
                     /locus_tag="PKI94_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25365.1"
                     /translation="MLLHFMKEATMTGVSAVKKIIFIPFENGVRKFVVDAAESIKKPP
                     MTKAQEKALLEELTTSPADTFTKVNEVIIHAVQPKVKSPLVQKVIGETYLPYEGGIRT
                     QEVTRNVLAPEDFVRYQAEGRILNIKV"
     gene            complement(45330..46427)
                     /locus_tag="PKI94_01090"
     CDS             complement(45330..46427)
                     /locus_tag="PKI94_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW25366.1"
                     /translation="MEILKKKNQVECFTTPSHSQSFFIFSKLRNFYKYDISETEAQNP
                     EAALFCAQKSAAKIYKTQSTHFLTNGSTSGIIAAVLACTKKHEDLRVPPRPLRERVGV
                     RGEKFLIWDKAHRCHNSSVILVGAQPVFYEYEFDKDWGVYTIPKLNQIEEILKKEKIK
                     ALIITSPTYEGVVCDVEKISKLCKKYKTYLIVDEAHGALYPFCDKLPKSAIYQGADFV
                     IHSLHKTAGGLNPTALLHCNLKDIDIQSALALISTTSPSYPMLASIEKNINFLNSKKG
                     RAKILELIENIENMKKNLPNLEFFKGDSTKILTKHPELSGFELSKILFEKYKIEDEIS
                     NQKSVMFLTGLGTSAKKLAGLEKMLKKINIS"
     gene            complement(46452..47444)
                     /locus_tag="PKI94_01095"
     CDS             complement(46452..47444)
                     /locus_tag="PKI94_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNW25367.1"
                     /translation="MEKIKVLLVDDEKDNLELLYRTLRHDYLVEKTTSPLEALEMLDT
                     KYFDIILSDHKMPEMDGVEFLKRSCELSPQSVRLLVTAYSDANILIDAINEGKIFRYL
                     KKPWEPAELIMVMQTAVDFSKLKQENDRLIYDLKKLFSGTVNAIIEALDAKDSFTLGR
                     SRRVTMLSMLVAKKMNFPPEEIGKLELAGLLHDIGMIGVSEEILNKIEALTPYEYEMI
                     KKHVEYGVRILEDIKQLKEVIEIIKTHHEKYDGSGYPLGIKGDEIPISARIIAVADAY
                     DSMVSQRSYRQGLSHDEAIKRIELEKGRQFDPDVADVFLEVIPEAIKYLDEAND"
     gene            complement(47431..48807)
                     /locus_tag="PKI94_01100"
     CDS             complement(47431..48807)
                     /locus_tag="PKI94_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW25368.1"
                     /translation="MTNKKKCLLIRMLWNDFKNSEVIMKNIGKKKSNREKLSIGTTFA
                     LSMSLLVAVATIGMAVYAVFFVDSIFSFFVPQDSMYSTILTFRENLQRIVIAFLIFGL
                     ILSYYMTKIIISPLEKLINGTKELSEGNLKYRVEFTKYTEINQLVQTYNQMAENLQSL
                     YDNLEKKVQERTKELKLAYEELENTQIIMVHSEKMRSLGELVAGITHEINNPINFIHG
                     NMTHLKNYSKALIELIEVYQNYENDLSEEKRDTLNKIKQEVEYSFIKEDLPMLIQSCQ
                     EGTERTKNIILDLKNFSRIEELVVNEIDLKREIETILNILHNKIKYKAEVIKEYDQDF
                     PIIEGYGGQLNQVFMNILDNACYAIEKKGKIYIRLKKEQDNVIIEIEDTGSGMSEDQL
                     KKIFDPFYTTKPQGEGTGLGMSISYKVIQNHGGQISAHSQLGKGSLFRIKLPVVLDKK
                     RELSYGKN"
     gene            complement(48761..49072)
                     /locus_tag="PKI94_01105"
     CDS             complement(48761..49072)
                     /locus_tag="PKI94_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25369.1"
                     /translation="MSKNQLKNKHKLAAVFASWTILIAGALSLTGSMSLDYYSVFATL
                     KKVIPASFAMGFIGWMMGSILDKPRRRRPVFHNSLFIKEVTKKENDEQEEMSFDSDVM
                     E"
     gene            complement(49069..49665)
                     /locus_tag="PKI94_01110"
     CDS             complement(49069..49665)
                     /locus_tag="PKI94_01110"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNW25370.1"
                     /translation="MIGLLKKEQAINIVPQDFKDATRGKVVDMKEKGFSVELNFEPQN
                     IPKGKKSEFYSSTENGILYFEAKVLDVEGKILKIENPVKHRYLQRRQFTRIKFINEMA
                     LHSNNKVIKVNSLDLSAGGMKIQSKDSLDIDTEYMTSIELFGDNEIRCLFEPIRIEKS
                     DDGIYTVSGRFKNMSNVAKMTLVQLCIKKNLETVNKKS"
     gene            complement(49678..51756)
                     /gene="flhA"
                     /locus_tag="PKI94_01115"
     CDS             complement(49678..51756)
                     /gene="flhA"
                     /locus_tag="PKI94_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNW25371.1"
                     /translation="MKNNDVILAIGLVTIVAMMVLPIPPGLLDFLLTINISLSIVILL
                     VCLYTKEPLDYSTFPTILLIATLFRLGLNVSSTRLILLYGEAGNVIQSFGEFIVGGNY
                     VVGSVIFVILVIINFMVITGGATRVAEVSARFTLDSMPGKQMSIDADLNSGLINDEEA
                     KARRRKIEREMNFYGTMDGASKFVKGDATAGIVITIVNIVGGLIIGMIQLKMNFLNAV
                     STYTLLTVGDGLVTQIPALLVSTSTGLIVSRASGQDASLSEDIRKEMFSDPRVLGVVA
                     GLLCFMGMIPGMPTIPFLSIGMITAIFAYYKIQDNKKVAEAELKQKEEVQKQERLGKR
                     KKKATRESVLELLAVETIEIEVGYRLVPLLNIEQGGDLLERIAQIRRQTALDLGIVLP
                     SVRVRDNLQLSPNTYQIKLKGIAIESGEVYPERSLAMNASGVNNDSNLTGINAIEPAF
                     GLPALWIEEKDKDYAETYGYTVVSPSAVISTHLTEIIKKNSADILSRADVQQLIDNLQ
                     KEVSEDYVKDLMKDLTAADVQQVMQNLLRERVSVRDLKTILETVSLQAKISKNYDFLT
                     EQTRQALARQICKQNLSDTGELFAITLAPDVENTIAQGVSPDGQSLTLDPDFTRRLLD
                     TLNSELEKAILSSGNQPVILCSSAIRLAFRRLIERTYPQITVMSYNEISSNVKAKSVG
                     IIKAVSQAVA"
     gene            complement(51855..52931)
                     /gene="flhB"
                     /locus_tag="PKI94_01120"
     CDS             complement(51855..52931)
                     /gene="flhB"
                     /locus_tag="PKI94_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNW25372.1"
                     /translation="MGNQAAERTEKATGRRRGKERDKGNISKSQDLISALMITMGVAL
                     LSIFSGYVLSNLKNLFYYTFTHLNPKDISTDDILLVFLPFAKTAGQIILPFLLFLMVM
                     GTIVIRLQIGHVFAKEKIKLKLSNISPMKMLQNAKKLFNPVEPRTIVEFVKSLIKLAV
                     VGMCGFSVINGRKDELFGLLGMDIEPAFAIIGSILGQMVVNICLAMLVLGLLDKKYQD
                     YEYEKSIKMTKQEVKDEMKDTEGDPKIKAKIRSIQVKMARQRMFANIPKADVVVTNPT
                     HYAVALKYDKMESPAPRVIAKGVDYVAFRIREIAQENNIPIVENKPLAQALYKLVPLD
                     GVIPADMFVAVAEILAYVYNKDKN"
     gene            complement(52955..53737)
                     /gene="fliR"
                     /locus_tag="PKI94_01125"
     CDS             complement(52955..53737)
                     /gene="fliR"
                     /locus_tag="PKI94_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNW25373.1"
                     /translation="MPTDLLVLLSPYNLILFVVVFTRLGGLMSSAPIFSTYPIPMQIK
                     VWFVALITFIIYPIVLVKSGFQAPSTIPELSVILLKEFMIGYVIGFISNLVFVGVEMA
                     SEIISIQMGLAAAQALNPLTGMSAPILGQGYVILTSMVFIGLNAHQWLFSAVYKSFAV
                     IPPGYGFIFNGQLTQEIVFLSGQIFSMALGIALPIFAVLFVTDVLLGFTAKMMPQMNI
                     FMVAMPLKIYIGLVMIIMFIAAMYSHIGVIIEKLFNAIAVIF"
     gene            complement(53746..54012)
                     /gene="fliQ"
                     /locus_tag="PKI94_01130"
     CDS             complement(53746..54012)
                     /gene="fliQ"
                     /locus_tag="PKI94_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNW25374.1"
                     /translation="MEQEVIISIMQKTFILILELSAPVLIVSITVGLMVSVFQTITQI
                     QESTLTFVPKIIAGILTIIILMPWMMSLFISNIHELFDAIPGLL"
     gene            complement(54025..54783)
                     /gene="fliP"
                     /locus_tag="PKI94_01135"
     CDS             complement(54025..54783)
                     /gene="fliP"
                     /locus_tag="PKI94_01135"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNW25375.1"
                     /translation="MKSGILKKMLFVLAAMLLFVPMAHCAMAFPNINIGMKAANTPQD
                     FSNGVQILIWMTILTLAPSIFIMATSFIRIIIVLALTRQAIGVGSLPPTQILSGLALI
                     LTFFIMSPTINKINETAIQPYMKNQITQQTALDKAIIPARDFMFKQTDEKNLALFIKM
                     AKIEKPKSKKDVPTYILLPSFILSELNTAFKIGFVIFLPFLVIDIVVSSILVSMGMLF
                     LPPTMIALPFKLIMFVMVDGWYLIVKSLVESFAT"
     gene            complement(54895..56412)
                     /locus_tag="PKI94_01140"
     CDS             complement(54895..56412)
                     /locus_tag="PKI94_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HNW25376.1"
                     /translation="MKKLFLTLAAFFCSLQSVFAFNLDKEIDKYVTPVSEKVSGIMFF
                     PVTLFGVEIPVIILWILIAGLFFTFYFRWIGIWGLKHSFEILSKPKEGNAEGEISPLG
                     ALMSALSGTVGLGTIAGVAISISIGGPGAALWIMFGAVLGMTLKFAEVSLSLKYRKFL
                     PDGTVAGGPMHFIAHGLTRRKMRWLGQPLAVIFAWLCIGGALTGGNMIQINQTTRQII
                     NIAGGETGILHGYNWLIGLICAILMGMIIIGGVKAITKVAMKAVPFMIYLYLFAGIII
                     LIANWHNLPYTFYIMVKEAFFPSSVAGGIIGIMIIGMRRSVQTNEAGTGSAPIVYAPA
                     QTDEPISQGFIALIEPFLTGLVCLLSAGIIVSSGVYKNYTTGVTGIELTSSAFESIFP
                     WFKYILSVVVILFALTTLISWAYYGQKAWNYIVGEGKKRTLFYQLGFLMFTVLGAIMN
                     VNTVINLTDSMMLGMAVPNCLAMYIMAKELKDDLKTYCKKYNVGTKFNKNWFKKA"
     gene            56597..56869
                     /locus_tag="PKI94_01145"
     CDS             56597..56869
                     /locus_tag="PKI94_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25377.1"
                     /translation="MLSIAGISRQVAFEKKQDKLNPKKTAPRKIAPSEDCNHLCYIAH
                     DGTEIPTDNPKALKIIQKINSDFHKKMKAKRPRSFKELVATKKIKI"
     gene            complement(56879..58024)
                     /locus_tag="PKI94_01150"
     CDS             complement(56879..58024)
                     /locus_tag="PKI94_01150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW25378.1"
                     /translation="MKILFVTDLYPVKQGEKNCPLTLHRFVANWIQLKNQVDVIRPNF
                     LLNSFLRGKVFHKNGFYEYENVRIFNVNYFSPFLFDITKKLPTEIDLKSYDIIVAHMP
                     SGIILANKIAKKYNIPLVCGVHCSDIEILTKLMYRFYFKKKLEEAYKNSKKIACRSFV
                     LQKKFCELYPDLADKTFVAPSGISVVGINSPLPYPPPQGGRRTDKTPTYEPLTSHLSP
                     LTIITCANLIKRKNVNKLILAINDLENFELKVIGEGKELSKLRKIASKNVKFVGKISN
                     KEVLEQMKNSHIFILPSVKETFGMVYLEAMASGCITVCTKDDGIDGIIKDGENGFLCE
                     PTIEGIKEILLKIKNFEDKEKIVQNSINTAKSFCEQNCALNYLENIK"
     gene            58090..60336
                     /locus_tag="PKI94_01155"
     CDS             58090..60336
                     /locus_tag="PKI94_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNW25379.1"
                     /translation="MANVGKKLSVAFYWHFHQPPYQLSEESDYLMPWVRLHALKDYLD
                     MLLPIKDFPKLKLNFNFVPTLLDALIEYGEKGYHDIHSRLTVTPIEELSAEDKEFILN
                     NFFDANYTSMICPNEPYDRLYQKRCKPEGTGIEDFSNQEFADIMAWFNLAWIDPVFKE
                     MFPELLVLLEKGENYSLEDRIKIIDFHRKIIKMIIPTYRQYLEQGKIEITTSPYYHPI
                     LPILLDVKSAQKNLSTPDTDLTDLGMEEDAKLQTKMALDRIEELFGKRPKGIWPPEHA
                     MSPDELEMLKELGVEWTISDEGILSNSIDFEFVRDFKGYLEDPYFLMKSYKYKTKSSD
                     IKLIFRDSVIPNLISFEYSNHDSKLAASDLYDRIKVIQNKLKSSPDENHLLTIAMDGE
                     NCWENYPQDGMIFLRKIYSLLEKDDTIETVLISDYLEKEKNHKKLDKIYTGSWINKNF
                     QLWVAEPVKNIAWHFLKRAKDDFEELKRTNPDNPDIEAAHKELMIAQGSDWFWWFGEP
                     NDSGQDHVFDYMFREHLKNIYRYFNADYPQRLDDPFLSVSIEPVRYPKCNFTPAIDGM
                     DFPEDQWENAGCINMPDGPSRQENKLFNRICYGADKNNFYLRIYLNDFFQETFLDSMF
                     LSQLYVYMKNGNRTQPLSPMRLVNQTENIFPIMKEKFHNEIRLSIINKKLYSIRLIKS
                     MPGGVWVAKHSKDMKVAFEQVFDVSVPFDDIDIKSGEKMEFFFANANFGIKDTFVPQD
                     HLLIIQRP"
     gene            complement(60592..61509)
                     /locus_tag="PKI94_01160"
     CDS             complement(60592..61509)
                     /locus_tag="PKI94_01160"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNW25380.1"
                     /translation="MLLIGENIHIISKSVQNALIERDEFFILDLIKKQSHLDYIDLNV
                     GPAKNQMQGILPWLCKLVEQNSNLKISFDTTNFDEMKAGLQKSKSPQNCLLNSTSADE
                     EKLEKMTSLTADFGSNLIALTMNKQIGIPKSADDRLNLAFEIIEKTSEKGIENKKIFF
                     DPLILPVSVAQDQAQEALNTIQMIKQSFEPEVMTTIGLSNISNGSPAEIRPLINKIFL
                     ILAYGAGLDSAIINSLDFDLINTVKMLETSKPKSSTDELCINLSEMIRDFGQIEDVAF
                     DTQDIEQVKIIKTAKILLNKEIYSHSFLN"
     gene            61627..62118
                     /locus_tag="PKI94_01165"
     CDS             61627..62118
                     /locus_tag="PKI94_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25381.1"
                     /translation="MNKKIFAVLLGLSMSLPVFASKLEGQVNKQTSPIIIQEQELTIG
                     TVQKYVHNGMSQDEVAISLGSPNIVTQDSNGKETWIYDKVASSITSRDKMESMIENDM
                     GARYFWHGVLTFVTLGLINKDVKPEMKDVAQLVTTQKTLTIVIKFDSTNKVESYTYHM
                     SKF"
     gene            62131..62649
                     /locus_tag="PKI94_01170"
     CDS             62131..62649
                     /locus_tag="PKI94_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25382.1"
                     /translation="MKKYLILLLISLVIFVALPVFAAKAPVQQATQLEKRAYQTRSFD
                     GLDKATIMKAMLNVLQDEGFIVNNANPLLGFISGSKEFDVTDKTIDVQKEFGIKKGAL
                     AMKGIRVATIEATANVSEFGKEIRVRINFKRKLLNTYGNAQFIDEISDEKYYQDFFSK
                     VDKAIFIQRQKI"
     gene            62663..63271
                     /locus_tag="PKI94_01175"
     CDS             62663..63271
                     /locus_tag="PKI94_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25383.1"
                     /translation="MKKIISVFLALTFSFYCVLPVSAAKIKVAKSQLEVREIQTHIFD
                     TNNINEVFKAAINTLQDEGYSILNIEDELGYIQARKEYKAVRIDKGRMTGYYFLLAEY
                     IALTALTYGMEAPIMADAIRKISNERSPRTVVVDSNVTIEPYGKKTKVRFTLIEKELE
                     NADGYSYIKSSPRKVLRIYTEPIYKEFFAEMDKSIFYGNAKL"
     gene            63297..63383
                     /locus_tag="PKI94_01180"
     tRNA            63297..63383
                     /locus_tag="PKI94_01180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63331..63333,aa:Ser,seq:gga)
     gene            63873..>64155
                     /locus_tag="PKI94_01185"
     CDS             63873..>64155
                     /locus_tag="PKI94_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25384.1"
                     /translation="MNELTETQKIIPEHAKKIALAKMDLMKQWSEFRKKSNNKFKADY
                     EFVQLYNTSNSNIFSILGKISRGTLHRWKAKLSDSEDYTKLLPQYKYSSV"
BASE COUNT        21196 a        11594 c        11379 g        19886 t
ORIGIN      
        1 tcttttcttt ttttcaatcg caagaaaaaa agaaaagaaa tgaggcatca aaaatagttg
       61 gtgagaattt tctagtttat aattttttgg attgcttcgg ggttttcaac ccctcgcaaa
      121 gacaaaagtt attaaaatat tattaatatt aagcttgcgt aaaaattgag atttgctttt
      181 aaatttgcat tgactttagc cgaaaaatca ttaaaaactt tagcaagata caccatgtgt
      241 atgaattttt gacaaaaaat tcatatttca ggttaaaaat ccgacgtaaa tcaatagtat
      301 aaacgtcaaa aagaggtcag ctatgaagat aaacgcaggt gtgaatttta tcgaaaacgg
      361 gcttacggct tcgcttcgag cgatgcatgt aaacagcgaa cttgtaggaa tgataaacga
      421 aaacgttatc ggatttgaca aagtaggtta tcagagaaaa gagcctataa tttcatcctt
      481 caccgaattt ttaggtgtaa acggaatttc tgcaaccgtt gatgataaag tgggtcgtct
      541 taccgcaaca tcaagccctt tagaccttgc tcttgcaggc aaaggttatt ttcagacagc
      601 cacaaaaaac ggtgtaaaac taacccgcga cggaaggttt aaaattgata aattcggaaa
      661 tcttttgact accgatgatg caaaagtttt aggtaacaac gggcttgcga taaaatttcc
      721 caacccgccc gaaagcctaa aagatgtaat tgtcggctca ggcggaaaaa taagtttatt
      781 caacaaagca aaaggcaaag tcgaatatgt aggaacttta ggcgtagttg attcaaacgg
      841 agcggctgtt gtcactcccg atgtaaaaca aggttatact gaattctcaa acgtttctct
      901 tgaacgcgaa tttctttcaa tgatgcctat aataagagga tttgacgcta acaggcagtt
      961 gtttatgata cagagccaaa acctcacaaa agctatttct caactgggca gtgtaagcta
     1021 attaaaaaag gaataaaata tgtacaacat ccaaggaatt atcagaaaat ctttaaacaa
     1081 caccacaacc caatttgaca gattgggcta tgttgcaaat aattttgcca attacaacac
     1141 aaacggatat aaatcggtaa gatttgagca gatgcttcaa gaagacggtt acttaaaagg
     1201 tgcagtcaga acagattatg aagtaggtat gatgcaatcc accggcaatc cttatgatgt
     1261 tgcaataaac ggacaaggct ttattccggt aacttccact tcgggtgaag tcgcatatac
     1321 cagggacggc tcttttaaaa aagggaaaaa cggttatctt ttaacaaacg ataactggct
     1381 tgtcggcgaa ggaataaaaa ttccgcctaa ttgctataaa tttgccataa aaccaaacgg
     1441 cgatatcgtt tcttacgatt caggcggaaa atccggagaa atattgggca aaattcctct
     1501 tgtgcaattt gccaatcccg aaggactgac acaaggtgac ggcaataaaa tgaaagcatc
     1561 ggatgaatcc ggagaagctt ttttggtcaa agaacataat tacatcgcac agggctcaat
     1621 cgaaagggca aatgtaaatg tttacgactc ggtttctgaa atgctcaggc taaacgcatc
     1681 aatgattgca agtatgcgta tggcaaaggt agttgacgat atgtacaaca aagcaattaa
     1741 cataagacaa tcatagcgga tttcggcaag tgcgccgtga cacgaagtgt cccagcacgt
     1801 ccggatgagc aaaaccacgc ttttgcgaat cgccgataat ccaagataaa aataaggaga
     1861 taaaaatgac attcacaccg atagattcaa tgggcaaagc atctttaatg atggatttga
     1921 ttgcacaaag acaaaaagca atcggttcaa accttgccaa tgttgacaca cccggatatg
     1981 tcagaaaaga cataaacttc agccaatatt tgggctcaat ggacagccct ttagaaacaa
     2041 aactctcagg caaacttgga ccttcggcaa tagtggaaga ccgaaattac gaagaaatta
     2101 accctgcaaa cgaacttatg gaacttcaga aaaactcgct tttatacaca atggcaacaa
     2161 gaagaatgtc aaatataata acgcagatga gaacagtaat taacgtagga aaataattat
     2221 gggaatactt gattcaatga acatgggctc aaaaagcctc aaagtacaca gcttaagact
     2281 tgatattgcg gcaaaaaaca tcgcaaacgt tgacacgccc aattacgtaa gaaaaatccc
     2341 tgttttaaac gcaacagaag acatttcttt ccacgggctt atgaacgaga tgaaaaacga
     2401 tgttttctca acaggtgcaa tgccctatgt tcaaggcgga gttgccttta caggggttgt
     2461 tgaagaccct acaatgggtg aaaaaattta taaacccgga caccccgatg ccgacaaaga
     2521 cggttacata agagcttcaa acgttaaccc tatggttgat atagcagatg ccatgcttgc
     2581 acaaagggct tatgaggcta accttgcgct gataaatatc acaaaagcaa tggcgtcaag
     2641 agcaacagaa atcgggaagt aattgataag ttgaagagtc gaagagttga atagttgaaa
     2701 ttattataat tgcacttatt cacctattca cttatttact tattcactct tatttaccat
     2761 tcactaattc aacttttccc gttgcaagat tgtacagagc tcctttgagc atgatttcgc
     2821 ctgttttgac tttttgtgca aggtattcgt ctttcttaag taaatcccga agctggtatt
     2881 ttatatttga aacaacagca caatgaagaa aatcttcact gtcttctatg cattccgaac
     2941 tttcgatagc cggataaata tgctccatta ttgcttttaa attttctgat attttactgt
     3001 catctttttg ggtttctatg gcggctctta tcgccccgca attttcatgc cctaaaatta
     3061 taactaattt tactcctaag gttttaaccg catattccaa acttcccaaa acttcttttg
     3121 aaagaacatg tcctgccgtt ctgacagaaa aaatatcgcc cagccctgca tcgaaaatta
     3181 tctctatggg aactctggaa tccgagcacg aaagtacggc aacaaaaggt ttttgttgtt
     3241 ttaataaaac ttctcttgtt tcctcaaacc tgttggggtg aatagatttg ttttgtgcaa
     3301 accgttcgtt tcctgctatt aacttttcca acgcctcttg tggtgaaatc ataaaatcct
     3361 catcttaaac acttttacct taaacactct tcaatggcaa gtttgtatga cattaaatct
     3421 cttgctgcga ttgatttggg aagacagttt acaccaagct gcgatgcaat atttcttatg
     3481 tcgtaagttg cagaaattat tatgattgga acactttggt attcttttaa gttctttacc
     3541 tgttcaataa gccattcccc tgcgtatttg ggctcaaaat caagttccat ttgcaggtca
     3601 gaaattatca gcgcataagg aatattgagg ttattgtaaa ccatatcata acccccactg
     3661 gcgcaatttg cctcttcgag ggcaaattgc gccccgaaaa gcttttgtat agtttgttta
     3721 tggtattcaa tccaacctct tgaatcgtca actattaaaa ttttcttttt aacatccatg
     3781 aaattattat accaaattat taaaacagaa taaatatgtt gcccttattt gtgaaatttg
     3841 gtaaaattta tactgtgaag gattgaaaaa atcattcatg tgtgttacta ttgtagcaga
     3901 attgtgttta tattttattt aggggtggaa atggacatca gacctgtatc tttgaatttt
     3961 ggcaatagca aaattacttt tcaaaaagca cttccggctt ttaagttaaa caatttggac
     4021 ttattggata taacaaaaaa agtgcaagaa tacaaagttg taaatcaaaa taacgaagta
     4081 ataatccatg agcttaactc ggccggaaaa aggattttaa aatgtttgta taaaaggata
     4141 actgatactt tgtcgcctga ttacggcaaa ttatggctca gggaaaaaac tgcttattca
     4201 catattgacg gaaaaataag cagaaaaatt ttatacaata aatacggtga agttgtagag
     4261 gcacaagctt ataacggaaa tgccagacct tatacatatc atcttaatcc agatttcgtt
     4321 taaaagtcca tgtcgcaaca caaagggtca aaactgctat aaaaataagc ggtataatgt
     4381 tttgcgctac atcatgcagt ggcattgctt ttagaaaaag acctttcatt attacaagaa
     4441 aaaatctgat aggattcaca taggtaaaat attgaaaaaa tatcggcatt tcctcgactg
     4501 gagaaataaa accggagagc aaaatagccg gcatttgaaa agtaaaagca ccaagaattg
     4561 cctgttgttg ggttttgcag attgaagata taaaaagtcc gacacccaaa attgataata
     4621 aataaaagaa cattcccatc caaaaaagcc agattgagcc ggtgaaagga atttggaatg
     4681 caaaagttgc cgcaagtgtc attattgaag aaagaactat agatatgatt aatggcggaa
     4741 cagtttttcc tatcaaaatt tcaagtgatg ataaaggcga gactatgagt tggtcaaaag
     4801 ttcccaattc tctttctctt gcaatggata gagcggttaa aacaagcgcg attgtggttg
     4861 caagaattga aataagcgaa attacagtgt accagaggaa aataagatta gggttatacc
     4921 agtttcgcac ttcaagatta attctcggta cggatttata ccttttgggg aaactttttg
     4981 cctcgtattg tgctattatt tctgaggcat aaccgtttgt tatcgcggcg acattggttt
     5041 gtcttccgtc aactatgact tgaacatcag cggttttttg ggcaagaagt tttgtagcaa
     5101 aatccgtgtt aaaagaaagc cccatttgaa ctttttgctg tcttataagc tttgcaagtt
     5161 cattttcgtc ttttacataa attatttttt taaaccacct tgaatttgca aaatccgctg
     5221 taagttctct tgattgaagg gtattactct tgtcaaggac agcaacatga aggtttctga
     5281 cctccatagt tgcggcatga gcaaagataa aaagttgcaa aagcggtgga acaataataa
     5341 gaatacgact tctagggtca gcccatattg caataaattc tttttttatc agtgataaaa
     5401 ttcttctgaa catttttaat cctcaagcct ggttgccgtt tttttatata caacggcaaa
     5461 caaaattaca ctcagtgttg ctaaaaaaag acaattcggt aaaattatcc cccaaaccgt
     5521 gcctgccata aattcatttt gaatagatga aaccatgtat cttgcgggca taaatacact
     5581 aagtatttga agcggaagcg gcattgaatt tatcggataa acaagccctg acaacatcaa
     5641 agcaggtaaa aatcctgcta caagagctgc ctggcttgca ttatattgat cttttaaaag
     5701 ggttgaaatc aaaagtccct gccccattgc ggttaacaaa aacagtccag aaaaaagaaa
     5761 aagcacaaga aagcttcctt tgaaaggaat ttgaaaaacg cttatacata aaaacacgca
     5821 aaaagacatc gaagacatcc ccaaaataaa ataagaaatg tattttgcaa gcactatatc
     5881 tatttttctc actctcgtgg caagaagtgc ttccatagtg cctctttccc actcccttgc
     5941 tattacaagt gcggtcaaaa gcattccgat aagagtcatc gtaacagaca aggagcctgg
     6001 cagaacaaaa taatgactgt ctattttttg attataccaa aatcttgtct gaacgttaat
     6061 caaaggcggc ggaattttct ttgaatactt gcttgaataa gtaagccact gctgaagaat
     6121 tgattttgtg taattttggg cataattggc aaggttaact tccgaaccgt ctgttatgac
     6181 ttgcgccgat gctatttcac ccctgtcaag tttggttgaa aaatcgttgg gtattactac
     6241 aattcctcta atgtgtgaac ctgttaaatc atcgtacatt ttatttttat cctgataaaa
     6301 tatcggttga acatacttgc ttcctttaaa agaatctata agtgttacta cttgctgtga
     6361 agtgtcgtcg atttttattc ccatatgaat ttttactgta tcaaggttta ccccgtatcg
     6421 gtaaattatc aaaagaataa gcggcagcac aaatgctatc agaatactgc tggggtctct
     6481 gaaaatttgg ttaaattctt tttttataag tgcttttagt atttgcatcc gtttattttg
     6541 accttttctt ttttacccct cgccccttga gggagagggt tgaatttcag cacgagagct
     6601 tgcttgagtg cttgaaatta gggtgagggg tatttttgga ttgccgcgtc gctcattctt
     6661 cgctcctcgc aatgacacat ttctaatttt cactttgttc aaaaagaact gcgtcactta
     6721 ttcacctatt tacttcttca ctcctttcta tcaacgtgac aaacgcctct tccatgtttt
     6781 ttgcaccggc catgttttta agctcggtcg gggtgccaag tgcaatagtt tcgcctcgat
     6841 aaaacaggct tatcctatcg cagtattcgg cctcatccat aaagtgtgtt gtaaccatta
     6901 tggttacacc tttttgtgca agggatgtta tgtgattcca aaactctttt cttgaaatcg
     6961 gatcaacccc tgaagtcggc tcatcaagga ataaaacagg cggcgagtgc ataaccgcac
     7021 atgccagaga aagcctttgt ttgtaaccca gaggaagttc tgaggctttg ctttttaagt
     7081 atttttcaaa gtggaaaata tctattattt cgtcgatttt ttcttttcgc tcctttaagc
     7141 ccaacccgta aatccctgaa aaaaatctta agttttgttc gacatttaat ccgccgtaaa
     7201 gcgagaattt ctgtgccatg taaccaagat gcgagcgtgc ttttgaagga ttttttaaaa
     7261 tatcctgccc cattatttta gctgtacctt ttgtaggtgt agaaagtccg cacatcatct
     7321 tgaaggaagt ggatttccca gcaccgttag gtcctaaaag tccgaaaatt tcaccctttt
     7381 taatgtgaaa agtattattt tttaccgcat aaaaatttcc gtatttttta accaaatcaa
     7441 gtgcttcaat tacgaattca ccgtcttgct ctcttaaatc ataacttttg gcaagctttg
     7501 attctttttt ttgataaccg cccataagct ttattacttc atcttcaaag ccctgtgaag
     7561 ttgagcttaa ctcctgaggt ttaccgttat aaattatctt gccatcattt aagacgatac
     7621 agttgtcaaa actgtgcgcc tcatcaaggt aagccgttga ccaaagaatt gtaatcccgc
     7681 ctcctgcgag ttcttttacc atttgcataa gttcacgcct tgaaatcggg tcaaccccga
     7741 cagaaggttc gtcaagcagc aaaaattcag gagttcccaa aagcgcacag gcaagtccca
     7801 gtttttgttt cataccccct gaaagtgctc ctgcaagtcg cggttgaaag ttttttaaat
     7861 ccgtaaaggt caaaagtttt tcaaaaatct ctgccctgtc ttttttttca atacctttta
     7921 aatccgcata caatgtaagg ttttcaataa cgcttaaatc ttcgtaaagc ccgaattttt
     7981 ggggcatata acccattttt cggttaataa gttccttttg tgtttctggg tttaagttca
     8041 atgttgaaat cgagccttcg gtgggaacta aaagtcctgc aataagtctt atcagggtag
     8101 tttttccagc cccatcagga cctataagcc ctgtaatttt acccttttcg atatttaagc
     8161 taaggttatc aattgcagcg cagttatctg aaaaattttt ggttaaattt ttaatttcaa
     8221 tgcttgatat catttttatc tttcattatt taatgaaaag ctttatgaag gtgaataggt
     8281 taataagtaa ataagtgaat aagtgcaatt tgttgcttcg caaccaaaat attaaccttt
     8341 cagctattcg cccattcagc tgttcagctt gattttaacc gtaaccggca tgccctgacg
     8401 caaatattcg tcaaggtcat agatgtaaac ccttatcctg taaaccaaat cggttcttaa
     8461 atccgttgtc tgaactgttt ttggagtaaa ttcggcaaca ggcgaaatat atcccacctg
     8521 ccctttatat tctcgttgtt ttccggtttg tgggtttttg gtatccgtta aaacttttgc
     8581 ttccatgccg tatttaacat ttgccagatt aggctcaggg atataagccc ttacccaaac
     8641 aggtttgttt tttgccatgg tataaatcgg ctgagatggt gttacagaag ctccaggctc
     8701 ttgaattctt gtcatgataa ttccgtcatc gggtgcgtaa attcttgtgt catcaaaatt
     8761 cttttttgcg cctttgctgg cggctgttgc cgcttcaaaa gaagcttttg ttgagttttt
     8821 ggaattgagc aagtcatcgc attcctgctt tgaagtggtt gaatctttgc aaagaggaat
     8881 atttctttcg tattttgaag ccgcatttgt gcttaatgcc ctatatagtt caatgtcggc
     8941 aaggcttttt tcataagtcg ctttgtaatt ttcatcttcc aacaaagcaa gaagctgacc
     9001 ttttttaacc gcatcgcctt cttcaaccag catttttgcg attttgcctt caactctgaa
     9061 gcccaaatcc acctgacgga tttccacgtt tccgtaaagt gttaaaaaat ctttatcgac
     9121 tttttttgag cttatggaat aaaaaattaa tccggacaaa cttatcaaaa atactaagat
     9181 tataattttc ttcatcataa attgcctcca actgctttgt atagggttaa ataatcaaca
     9241 agacggtttg tttttgaatt taccctgttt tgttgcgaca aaatcaaggc ttcatgttcg
     9301 cttaaaagct gcggataaga aattgttccg cgattaaatc tttcaagcga gcgtttataa
     9361 gtatcttgct gaatatttaa atttttcaag gtgtttttat ctatctcgct gtcttttttc
     9421 aaaacgaaaa gactgtcgtt tacttcttta agtgctgtta agtcattttg gcggtaagcc
     9481 tcaaacattt gttcatagcg gcttttgctc atttttaaat tggcgatttt tcttccgccg
     9541 gtgaacaaat ccaaagaagc tcccgcaaca agtgctgcga tagttgaacc ccatgtgccg
     9601 aatcctaatg agcccacgtt gctgaaagcg taaattcccg ttaaatttat tttgggcaaa
     9661 aattcttttc ttgcaacgcg gatgtcgatt tttgcctttt gaagcttggc ttctgttgcc
     9721 ataatatcgg ggcgcgaaaa aactacatcg gaagaaatgc tatcagggac agggcattta
     9781 tattcaagat tatcaaacga aactctttta aaatttgtat tttcaggaga ttctccgatt
     9841 aaaaccgcaa gctgggtcag ggttttttct cttgtcttta aaaagtcgtc gagcgaattt
     9901 tttgcgtttt cgacctcttt ttttgcattg tttaaagaag tggcagaaac cgttcctctg
     9961 tcaaatttct gttgctcgcg tcttaatttt tctcttctta tttcgactat ttgttcttga
    10021 agacaaattt gtttgtcgga tttgaccaag ttgaaataag cggttgcaac atctgaggca
    10081 agcactatgt aaatcgcttt ttcttcatat tttgatgatt cgtaagtttt tttcgaagat
    10141 tttgttttat cgcggttttt taggaaaatg tctgcttcgt agtttacctg aaaaggaact
    10201 gcaaaaatgt tgttctttgt tgaaacaaag ctgtcgggaa gatggtttaa aatgtaattg
    10261 ccgcctacag aaagcgaagg aagctcgctt ccgaactggt atttgaccgc ctgcctgtat
    10321 tcttcgacct tccaggatgc ctgccttgca ttgtggttgt tttcgactgc ctttataata
    10381 tattcggtta aaatcgggtc gttaaaattt tcccaccagc atgggtttat gtattcgatt
    10441 ttttcttttg tgtttatcgg gcacggacag aatttcttgc cccaaaaagc aaaacaggga
    10501 atctgtatga aaaaaattga aattaaaaat aaggcaaata ttttttttat tttttgcatt
    10561 ttacaccgca cccaaatctt tcagtattgc tttgacctgt cccattacaa tttgttttaa
    10621 catttgaatg tcttgtttac tatagcctga ttggttcatt agttttagcg aaaattgttt
    10681 aagaatttta gccgagtgga tttgtcccac tatggtaatt gttctgaaaa ttatttcttt
    10741 gtcattttcg tctctgccta aaatagaggc taataaacgc tttaaaacct tgtagccttc
    10801 tgcatttaaa ggtacacccg aggtcatctg ctctctgaaa ataatcatta tttcagaatc
    10861 cgagatttta tcggagtaaa aataatccat catcatatcc aaaaatttaa acagggcggc
    10921 tattttttct tcttttgaca aatgctcaaa actttgagga agctctttgg tacccatatt
    10981 attcataacg tactgaataa tgttttcgac tatacttgaa actacctttg aataaagttc
    11041 ttcttttccg ccaaaatagt aagaaatcat gctgatgtta acgcctgcgg ctttgcagat
    11101 atctctagtg ctgataccgt cgaatccctt ttttgcaaaa agttcggttg cactatcaag
    11161 gattttcctt ttggtttttt ggcttgaatc aggcttttta ggcataaatc ctccatattt
    11221 aaaactcaca tgctgcatta taaatcaaac atttaattta atcaagtgtt tgactttaat
    11281 taattttaaa attatttttc aggggcggtt ttgccttgca aaaccgcccc tgaaaaagtt
    11341 tagaatgccg aagacaggac ttggcttgca ccgttagcga ggagtatgaa actccgagcc
    11401 ttacggggca ggatgtccgc ttgcgggccc ctgcgctgtg cgagcagcac agccgcaggt
    11461 tcaaagtccc taaataaatc ttgaaaataa aaataaaatg ccgaagacag gacttgaacc
    11521 tgcacgagcg tttactcaca tgtacctgaa acatgcgtgt ctaccatttc accacttcgg
    11581 catgaagcta taattatttt acaacaaaat tttaattgta acaaagtgtt gcacataaat
    11641 taacaagttg gcaatagata aagaattaga ctaaaataga taatacacgt aatgttgcat
    11701 gcagaaggag tattatgaag attttaattt ccaacgacga cggaatagtt tcaaacggta
    11761 taaaagtttt atcagagaca ttggcagaag aacatgaggt ttacatcgta gcaccggata
    11821 gggaaagaag tgctgcggga cactcgctta ccctgcatac tcctttaagg gttgaagaat
    11881 tagaagccaa aaacgggatt aaacgctgct gggtaacgac aggaaccccg ggggattgcg
    11941 ttaaaatagg cttaagcgcg attttggaag aaaatgaaaa acctgatttg gtaatttcag
    12001 gaattaacca cggacccaat ttgggtgctg atattttgta ctcaggcaca gtaagttgcg
    12061 ctatggaagg ggcgatgttg gggtatccga gcattgcgat ttctcttgcc agcatgagat
    12121 gcgaacttga agatttcaaa ttcccggcaa aatttatcgc aagtttattg ccaaaattaa
    12181 aagaatttga ttttcctaaa aaaaccatat taaacgttaa tgttccggca cttgaccctg
    12241 aagatatttc aggagttgca ataacagaat tgggcaaaaa aatgtttacg gacaattatg
    12301 aaaaaagagt tgacccgaga ggaaaaattt attactggct tgcaggcgaa ttgatgaacg
    12361 agcccgaaga tgcaattaca gatattgcgg ccgttagaaa caataaaatt tcaataagtc
    12421 ccgttactta tgagatgaca agaaaagatt caatgaaaga acttaataaa attttatgca
    12481 gcggcgatgc ttgcgaatgg tattagaaaa gtaaaaagga gcgggcgcaa tttcattgcg
    12541 cccgctcctt tttactaaaa ttttatgtta taaatttaaa aaacacagcc attattgcac
    12601 cgacaatcat gcagtaatat gcaaagaagg cgagcgagaa tttttgcaaa aatttcagga
    12661 aatattttat acaaaaatag cctacaacag ctgaaactat tgttcctgaa ataattgatg
    12721 tccagttaaa agctgctatt tctttaaaat ctatttccaa aagcgggtag agcattgaag
    12781 ttccaagtat tatgggaata ctcaacagga aagaatattt ggcgcaagtt actctgtcaa
    12841 gccctgagaa aagccctgta gctattgtcc atcccgagcg cgaaaatccg ggaaacgccg
    12901 caagcccttg tgcaataccg attaaaatgg agttttttag atttatttta tctgtttttt
    12961 gcggtatttt tttggaaaca aattcgctca ataataaaac aatccctgta ataaaaagaa
    13021 gaactccgac tatagcgggc gagaaaacca atttttcaca aaagtctttg agcggatagg
    13081 cgacaaagat tgtaaaaaaa gttcccaaaa ttatgtacaa gcccagtttt gcttcataat
    13141 cggaaaaatc ttttgtcttt attgcgttga aaagcgattt tattatttga acaatttctt
    13201 ttctgaagaa aatcaaaact gcgataagtg ttcccagatg aagcatgatg tcaaaaaaca
    13261 cttcttcatt ggattgaatt acgttcagaa cgcccgtaaa gtatttatat aaacttgagg
    13321 tgaaaacaag gtgcgcagaa cttgaaacgg gcaaaaactc gctaagccct tgaactattc
    13381 ccattaatat tgcttgtatg atattcataa ttcctctatt acccctcacc ccaaccctct
    13441 ccctcaaggg gcgaggggga gtcaaagatt ccctctaatt tacataataa cttgcacaag
    13501 atgtcggggt ttcccagaca gaaactttgc ttacaatttt aattttttct ttcatttttt
    13561 catagataaa tttagcgata atttcgctgc ttgggcttat tcctttgaat tcaggcaagt
    13621 tattaatatc ttggtggtct aaaatgtcca aaacagcttt taattctttt ttgagaattt
    13681 tataatcaat caaaatattg cttttgtcga gcgaatttcc ctgaacaaaa acctccacca
    13741 gccagttatg cccatgctga ttttcgcaat caccttcgta attcaaaagg tgatgcgccg
    13801 ccgaaaaata catctggatt tttaattcga acatgccgaa agcccccctt tttctttaag
    13861 attaaccgat ttgctcttta gaataaaatc cgcaagttct cttacagcac cttttccgcc
    13921 gccttttgtg gaaacaaatt tgcagactga aataacttct tcaacagcat ctgcaggaca
    13981 acaagaaagt cccacttttt caagcacgca aagatcaggg atatcatctc ccatatacgc
    14041 tacttcatcg taagaaattt tgtatttttc gattatttca tcaagcgcgt ggattttatt
    14101 tttttgcccc tgataaagct ctgttattcc taaaattttt gccctgtgaa cgactgttcc
    14161 gttgtttctt gcggtaatta tcgcgggaat aacgcctgcc ctgataagca ttacaattcc
    14221 ctgaccgtct ttggagttgt atgttttgta ctccactccg ttttcgtcaa aagtcaagct
    14281 tccgtctgtc ataaccccgt caacatcaaa ggcgaccagt tttatttttt ttgctattga
    14341 ttttaattga atatctttta tcatttttgc tcctgttttg gtacgccctg cgcaccctac
    14401 tttataattt tgtcattccg catttattgc gggatctttt ttggtgggat agcaatccca
    14461 ccctaccgtt cactgctcac cgttcaccat taagcaactc ctgcttttaa taaatcgtga
    14521 atgtgtataa ctcctacggg aaaacctttt ttgtcgctta ttgcaagtgc tgtgatagaa
    14581 tatttttcca ttaaattaag agccttggca gcaagcgcat cgggtaaaac ggttttggga
    14641 ttaacagtca taacctcttt tgccttcagg ttttcaacgc ttttgtattt gattaaagtt
    14701 cttctgatat caccgtctgt caaaacacct gtcataattc ccttttcatc tgtcaaaatg
    14761 gcaagcccga gttttttatt tgaaatcagc tcaatcacat cggtgaattt atcgtcttgg
    14821 cttgcaagag gaagcttttc gcccgatagc ataagttctt ctactttata caagaagcct
    14881 ttgccaagat ttccggacgg gtgataaatt aaaaaatctt cttcagaaaa tcctcttttt
    14941 tcaagcagac aaaccgcaag cgcatcgccc attgcaaggg ttgcggttgt acttgcagta
    15001 ggtgctttgt tcaaagggca tgcttctttt tcgacagaaa catcaagcac cacatcagaa
    15061 gattttgcga ttgtggaatt taaaactcct gtcattgcaa ttatcggtac gtcaaatctt
    15121 tttaaaagag gcagaaggtt aagcaattct tgagtttgac cgctgtttga aattgcaata
    15181 ataacatctt cccttgtaat tatgcccgaa tccccgtgag tgctttctgc tgggtgcaga
    15241 aaatatgaag gagttccagt tgatgataga gttgctgcaa tttttttgcc cacaagccct
    15301 gatttgccca ttcctgtaac aattacccta cctttgcagg catataaaat ttcaatcgct
    15361 ttttcaaaat ttccgttcat tttatctttg agttttaata ttgaatttgc ttcaatatca
    15421 agaacttctt ttgccaaatc atttatactt tttgtcattt gcattttttc tcctgttttt
    15481 ggcggattat ggtttaactc tatcacagtt cacagctcac tgctcacttt tcactatttt
    15541 attatacgat aaattcatac aaatgtatta gttttataca aaagttcaaa tttttatttg
    15601 aaaagtgccg aaaaattcta catatacagt aaatgtaaga aggactatga cgcaaaatct
    15661 tttggaaaga aacttcatca atcctcctgc gcatgaagca tgcggcgcac ttgatattgt
    15721 tgagtcgatg aaaaaataca tttttgaaaa cgattgccct gttatgtcaa tggatgtttc
    15781 gcatttgaat gtaatagacg caagcaaagt agcaattctt tgttcgacat accactatgc
    15841 caaatatcct cacgggcaca tagactggtt tgtcaattct tccgagataa aaaaccttgt
    15901 aaaaccattg aatttaggta atattgattt aattacggtg tgatttttta caaattattt
    15961 tcgggttttt ttgtaaagtt tttttcggct tgctgaaaat aataaatttg tgtctctgct
    16021 gttctggaaa aatttttaaa gttaatattt tgtgtcatat ctgttttcaa ggcccaagga
    16081 taatttttat aaatatgttt atacattgca aacaatcttt ttgaagttac ggggtgtgta
    16141 gtaaaaaaat cccatgactc ttcgggcata aatttgtttg aaacacaaat agccgcaatc
    16201 ggattataac cggcttttgt catcaaatcg attcctttta aatctgccct gtattcatat
    16261 tctctggtat ttaacttcat cattaccgct tcaaaacctc ctttgtaagc atcaagtgaa
    16321 tgggctgttt catgcgacat tataaaggca agttcatcat cattatctat ataaggcaaa
    16381 atccccgaag aaacagtaat ggttttatta gttagcgagg catgagcatt tattactttc
    16441 ggtgttttgt caagattaat aggaatgcgt ttgtcaagct gatttgcatt gataattctt
    16501 gttccgactt caagaatttt ttgttgttcc ttgccgtttt tctcccaaaa attttccata
    16561 gtaacacccg aacttgattc gacaaaatct gctttaacag ttaaccctgc caataacaac
    16621 cccaaaatta acaaaaactt tttcataaaa tcctcctaaa ttcttatacc taaattttat
    16681 acgctgattt ttttaaaacc attagcctgt aagagaaaat acaaaaattt acaaaattta
    16741 atttttaaaa gctttaaaag taaataatgc aaatatattt gtttttaata atatatgaac
    16801 aatttatgtg tagataattt tagccctgtt tccaaggtta atttttgcgg aacgacaatt
    16861 ccgcaatata agcccttaaa acctttgagt caggatacat ttgagcgtac aacttcaaaa
    16921 aaagaaattt cttttgaagg ttggcctaaa attttttctg aagtaggtgc ggtaaccaca
    16981 gaagcagtgg caaaactgtc aaaggcggaa atgcagcatt tgacaggtgt cgcaaaatct
    17041 aaaattgatg aacaacttgt agatgatatt acaagatttt ctcaaatttt aaaaaaagga
    17101 ttggatgcta aatttaacaa aaaatatgtt tttgtatcaa tcggacaatc ccctgcggct
    17161 tttgccgatg ttcttaatgc cacgggagtt gaaaccgcga tttgcccgat ttcaaatctt
    17221 tctaacttaa aaaacgcctc accgcttcta agaaatccca atttatcaaa atattttgaa
    17281 tatatgaaaa aaataggact tgaccccaat aaaatgaaaa acagcggcaa gcaatatatt
    17341 ttcgttgata tttcatctac gggcaaaagt cttaaaattt tcgaagaatt tttaagggac
    17401 aaaaaaagcg gatttaatct tcctaatgta aaatttgttt cacttcagga tttgatgtcc
    17461 tcttgcaaac cgtcaaacag tgaagaagct tcttttatgg aagaattttt tgaagaatat
    17521 ataaaaacac ctgagcttaa aaaggaattt tcttcaatat tcaaactgga ttacaaagac
    17581 attgacaagg ttcaacaata cagagacaaa acccctgcaa acgaaaagct taacatgctg
    17641 aaacttttag ttctaaataa agtcttgggt agataatctt tgtgtaaatt ttaataaaaa
    17701 tttaatttga ttgtaaaata ttaatgatag aatgctatta ttaattcttt tcaaaggtta
    17761 aaagaggtgc gagcgggcaa agttaaataa aatcgaggtt cgcaagcaaa aatatagcga
    17821 gtgcataagg ctggtaagcc gatagaatga cagtttaata aatgtaacag ttagttagta
    17881 aaggcgcgaa taaaaaagtt tcgtgtaatg gtaaaatatg tgcggaatag taggatatat
    17941 aggtgataaa ccggttgttg acattttggt taacggctta acaagtttgg aatacagagg
    18001 atacgattct tcaggtgttg ccctacatgc agacggaaag attaaaatat ataaagcaga
    18061 aggcaaatta caaaacttaa aagatgtttt ggcaaaagtt acaaatgaaa caaaaacagc
    18121 caaaatgggc ataggtcaca tccgctgggc aacacacggt gcgcccaatg aaattaacgc
    18181 ccaccctcac acctgtacct gcggaaaact tgtacttgtt cacaacggaa ttatagaaaa
    18241 ttttaaagaa ttaagagagg aattaatcaa aaagggctgt aaattctgct cccaaacaga
    18301 cacagaaaca gtcgctcacc ttgttgcgca ggaatttcat aagacaaaag atttaacaaa
    18361 ggcggtaaat caggcgacaa aaaggcttca tggagcttat gctcttgtta taatgcatca
    18421 ggacttgccc ggcatgattg tcgcaaccaa aagaaacgcc ccgctgcttg ttggattggg
    18481 cgaaggtgaa tttttcgtgg catctgatgt ccctgcaata attgagcata caaacaaggc
    18541 actttatctc aatgacgatg agattgtaac cataacaaaa gaaaaaatgg ttttgtcgga
    18601 cagccaaggt aatgaactta gccaaaaaat agaagttctg ccctgggagc ctgtcgcgct
    18661 tagcaagatg ggcttcaaac actttatgct aaaagaaatc cacgaacagc ccgatgtcat
    18721 tagaaatgtt ttgagcggca aattgtataa tattgacgag ccgatttcat taaaagaagt
    18781 tcaactaagc aaagaaacac ttaaaaactt aaacagaatt cagattattg cctgcggaac
    18841 ttctttgcat gcggctatgg taggaaaata tttaattgaa gatttttgta acattgcagt
    18901 agatgttgaa ccgtcaagtg aatacattta cagaaaaacc gtcacagaca gcaaaaccct
    18961 tgttataggg gtttcacagt ctggcgaaac ggcagatacg attaccgcaa ttaagcaatc
    19021 taaggaaaaa ggctctcaca ttttaatcat aacaaaccgt cccgattcga ccatggcaag
    19081 agaagcagac agtttggtgc cgattaacgc agggattgaa gtttctgttg cggcgacaaa
    19141 atcttacacc gcgcagttga tttcttttta tctgctggca ctttatatgg cggaaattaa
    19201 aggaacactc gataatgact ttttaaccga gttaaaaaga gaacttattg ttcttcccca
    19261 aaaagttgaa acgcttttat cgcataaaga agaaattcaa aaatgcgcca aggcattttc
    19321 tgccaccaaa gattttatct atatcgcaag gggaataaat ttccccacag ctttagaagg
    19381 tgcgctgaaa ttaaaagaaa taagctacat aaacgcaacc ggctatcctg caggagaact
    19441 taaacacggg ccgattgcaa tgcttgatga gaccatgcct gttttgtcca ttttgatgca
    19501 gggcaaagtt tatgaaaaaa ttctttcaaa cgcggaagaa gcaaaagcca gaaacgcaag
    19561 gcttatcgca cttaccaatt caaaagacga aaaattgggc gaactttttg actttatttt
    19621 aagggttccc gaggttaacg agatattttc cccggttatt gcaatgattc ctctgcagct
    19681 tcttgcttat tacatcgcag aatttttagg aaaagacgtt gaccagccgc gtaatttggc
    19741 aaaatcggta acggtggaat gacacaaata aattcaaaat taatagaaat agaagtccct
    19801 gaagtgttcg acaatgattt tattgaacaa aaactttctg aaatgggcat tgagccttta
    19861 aggtgggctg ttgtaagcgg tagccgtgtg tggaagctcg aaggtcgggc tttgcccgcc
    19921 tgcagagctg taaccgatcg atggcgacgt aagcgtttgc attgcaaacg ccacaggagc
    19981 aatttggcaa ataagattat tttaactgtc agcgcagctt ttgaaagttt atgatatgat
    20041 attaaagtga atagtaaata gtaaattgta aatagatttt tccattcact atttcctatt
    20101 taccatttac tgttaaaaag gagataaaaa tgcttatgac acaacaaaaa tacgacatta
    20161 aagacataaa ccttgccgat cagggcaaaa accagataga atgggcgttt aaagatatgc
    20221 ctgttttaaa tcagattcag gaaagattta ttaaagaaca gccgtttaaa ggcttaaaat
    20281 tatcggcttg cgttcatgtt acaaaagaaa ccgcagcact ttgtgttgtg atgaaagcag
    20341 gcggtgcaga tgcgattttg gttgcgtcaa acccactttc gactcaagat gacgttgcag
    20401 cagcacttgt taaacactac ggaatcccga cttttgcggt tgcaggagaa agcgttgaaa
    20461 cttataaaga acacattaaa gaagcactta accataatcc cgatatcata atcgacgacg
    20521 gatgcgacat cgttgcaatg attcacgccg agcaccccga agttgcgcaa aaagtcatag
    20581 gttcaaccga agaaaccaca acgggaatcc aaagacttca agcacttgaa gctgaaggaa
    20641 aacttaaatt tcccgcagtc ggtgtaaaca caagcatgac aaagcattta tatgacaacc
    20701 gctacggtac gggacaaagc acaatggacg gaattatgag agcagcaaac attcttattg
    20761 caggaaaaac cgtcgttgta tcaggctacg gctggtgcgg aaaaggctgc gctatgaggg
    20821 caaaagcaat gggcgcaaac gtaattgtgt gcgaaattga tccgcttaaa gcacttgaag
    20881 cggctatgga aggctataga gttatgccga ttgatgaagc ttcaaaaatc ggagatattt
    20941 ttataaccgt cacaggaaac aaacatgttg ttgatgttaa gcacattttg aatatgaaac
    21001 acggtgcgtt tgtatgcaac gcaggacact ttaattatga ggttaatgtt cctgatttag
    21061 ccaacgaaac cgtcgaaatc aaaaaaatca gacctacttt agaagaatat aagcttaaaa
    21121 acggaaaatc ggtttatgtt ttagcagaag gcagactggt aaatttaatt tccgcagaag
    21181 gacaccccgc aagcgttatg gacatgtctt ttgcaaacca ggcacttgga attgagtttc
    21241 ttgtcaaaaa caaaggcaaa ttagaaaaca aactctatac ccttcctgtc gaagtggata
    21301 acgaaattgc aaaaatcaag cttgagtcaa tgggaatttc tattgacacc ttaaccccgc
    21361 tgcagcagga atatttgaga agctggaata tatannnnnn nnnnnnnnnn nnnnnnnnnn
    21421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnntcactt ataaatactg aaagaaacga ttttatcggc atttatgccg
    21541 agccattcgc tgtgtgaaac aaagtgtgat tcgcagccgc acttttgatc tgaacatttg
    21601 cataaatctt caagcagcca tgttttggcg tcatttaggg ttaaaatgca atcggattca
    21661 tcatcgggcg gacagtttgc aagcttacct tttattttta aatggtttgt cataattgca
    21721 acagtttcaa ccttatattt ttttgcaatg tgacaaattt gtttaaccaa ttcctttgac
    21781 ataaaaacct cctttaataa gattttaaaa gaattattta aaaatttaat cacctttatt
    21841 gctaatttaa aacaattgag gatacagaaa aattcgaaaa attaaaattt ccttgcaatc
    21901 tcatttttgt tgaaaacatt aatatttgta acaatttaat tttaatcggc aaaaaattgt
    21961 gattacgtat tttaaactta tacctatgat ttattaaaaa aggagtataa aaatatgaac
    22021 gtaaacacat taaatggttt gtcttttcaa gctaaaacac aaaacggaaa cgaatacaaa
    22081 aaaacagggt ttttcagaac agcacttcct gttttatctt cagccgcagt tatcggcgga
    22141 acttatatta acaaagacat tgatgcaacc tttgcaagaa aaagctttat ggaagaaatt
    22201 aaaaaatcag caaaaaagcc aaaaaacatt ttggtatttg cagcggtttt acttggcgca
    22261 ggtgtcataa attttatcat cggtgcagga tttgatagtt taatcaacaa acaccgctca
    22321 aacaaagccg ataaaaaagc tgttcaagcg taattgattt tttaaatatt tggcgggggt
    22381 tttgcaactg caaaaacccc gcctttgttt aattaccaca ctcgtcatgc cggaacttaa
    22441 aatgacgact ttgtaatgag tgcgcttaat ccggcatctc atttatgaga ttctgaataa
    22501 ggacgaccaa ttctgagccg ataacaaatg tttcaggacg acaggaataa tttattttgt
    22561 cggcattgca atgccgacct acattctttt caattctatt tactatttac catttactat
    22621 tcactccttc atttacccct ctttatataa tttttacttg attgtgaaga ttgttgtgcc
    22681 ataatatatt tgaacatttg acttaatttt ttagcacaga gagaaaaaat ggctgaatct
    22741 gacgaacaaa atttggtata tttattagac ggtaaaattt atattaattt gaccaataaa
    22801 tgcaccgcca attgtgtttt ttgtatcaga agcctcaaag atgacgtttg cggagctaat
    22861 ttaaggttaa aaaacgaaaa tttcggcgca caagaagtta ttgaccaatt aaaaaaactt
    22921 gtcaaaaatg agcgagaaat tattttttgc ggttacggcg agcccacttt aaaacttgat
    22981 attttaaaag aggtagcaaa ttttctcaaa acaagctata ataatgtcaa actccgcata
    23041 aacacaaacg ggcatgcaaa ctgtgtttac aagagaaata taatccccga attaaaaggg
    23101 cttattgatg aaatttcagt aagcttaaat gcatcaaaca aagaacaata cgaagagctt
    23161 acacagccga aattaccttg cgaaaatccg tttgaagaag tcaaggattt catcaaaaaa
    23221 agcgtcgaag agggaattga aaccaccgta acggtagtaa acggttacaa aaaccataaa
    23281 atagacctga atgcttgcga aaaaatagca caagatttag gtgcaaattt cagagtcagg
    23341 caatggctcg acgagggtta ttaagcgaca taattcttgg cgaagaatga gccttagaag
    23401 tatgtcgttg cgaggagaga gcagggcgaa cgacgcggca atccaaaaac ccctcaccct
    23461 aaccctctcc cgcaaggggc gaggggacaa ataaaaataa tattaaaaag taagaatata
    23521 aaagaaaggc aaacgaacat gaaacagtta gacaaaatta tgaaaccaaa atcagtagcg
    23581 gtaatcggag cttcaaccaa gcctttggca atcggctctg aaattatgct caggttaaaa
    23641 gaatacggat ttaaaggcga tatttatcct attaacccta aagcagacca aatcgaagga
    23701 ttcaaagcat atcccagtgt tttggatgtg ccgggtgaaa tcgattttgc ggttgtggtt
    23761 atcccaaaac aattcatact cgacaccctt gaccagtgca accaaaaagg tataaaaggg
    23821 ctttgtatta tatcatcagg cttcaaagaa gtaggatctg aaggtgccga acttgaaaag
    23881 caggttcttg caaaggctca ggaatacgga atgagatttg tcggtcctaa ttgtttggga
    23941 gtattaaata caaacgataa cgtaagactg gatgcgacat ttgcagaagc cttaccagaa
    24001 aaaggcgggg tagcttttgt atctcaatca ggcgcattgg gcggcggaat tttaaatata
    24061 ttaaaagatt taaatttggg attttcccaa tttgtttcaa tcggaaacca ggcagatatt
    24121 aacgccgaga ccatgatgga atactgggaa gacgatgagg acgtaaaaca aattttgctt
    24181 tacatggaat caatcgcaaa ccctgcaaat ttcagaaaac ttgcaacaag aatttcaaag
    24241 aaaaaaccta ttatagcact caaatcagga cgttcagcgg caggtgcttc tgcagcgtct
    24301 tctcacacag gctcattagc aggtgcggat aaagccgcaa acgcactttt ggcgcaatct
    24361 ggcgttatca gagaatactc tttgaaaaat ttctttgata ccgcaaaagt tttttctaat
    24421 tgcccgcttc ctaaaggaaa cagggttgca attattacaa actcaggcgg ccctgggatt
    24481 atggcaaccg atgcggtttg cgaacacggg cttgaaatag caaaaattaa agaagaaaca
    24541 aaagattatt taagaagttt tcttcccgat gttgcaagcg ttaaaaatcc gattgacatg
    24601 atagcatcag ctccggctga acactaccaa aaaacactta cagcggttat tgaagacgag
    24661 aatgtcgata tgattatggt tattttcctt ccgcttatag gtcaaaaaga tattgatgct
    24721 gcaaaagcga ttatggaaat taaggaaaaa tatcctcaaa aaccgattat cggagtattt
    24781 ataacaaaac aggaattttt tgcaaaagtc gccgacctaa accccaatat gccgtttttc
    24841 atgtacgcag aagaagcagc agacgggctc tacaggcttg accagtacag aagatggctt
    24901 gaaagaaaag aaggcaaaat gccttgcttt gaagttgata aggcaaaagc tgaaaaaatt
    24961 atcgctcaat caatatcaga aggcagagaa caattaacaa cattagaaag tatcgatgtt
    25021 cttgatgctt acggcataag agcatgtaaa tacgggcttg cgacaaacga agaacaggtt
    25081 gttaatttag gaaacgaaat cggctatccg gttgtaatga aaatgacttc caaaacaact
    25141 tctcacaaaa cagatgtcgg cggagtcaga gttaatatcc agtctgaaga ccaactgaga
    25201 gcagaatacc aagatcttat ggcaaaatta aaagaaaaag gtttgcttga agggcttgaa
    25261 ggggtgatta ttcaagaaat ggtaaaaggt aaccgagaaa tggtctgcgg tatcgcaaca
    25321 gaccctcaat acggaccgat gcttatgttc ggacttggcg gggtgttcat tgaagttatg
    25381 aaagatgtaa cttttagaat tgcgcctttg actgacgttg atgcttcaga aatggttcgc
    25441 tcggttaaag ccttccacct tttagaaggt gcaagaggaa caaaacctgc tcaaatcgag
    25501 caaatcgaag aaactttgtt gagattgtca cagttggtta atgatttcaa attcattgac
    25561 gaacttgaca tcaatcctct tcttatttct gaaaaaacag gcgaaggaat agctgttgac
    25621 ggacgtatta aagtaagaat ggcagaagct aaagaagcaa taaactgcaa ttgttcttgc
    25681 ggatgcggct gcagctgtaa ataataaata acaaaaagag gcgggaagtt cttaaaagaa
    25741 cttcccgcct cttttattaa aattttgttc gaagtataat gaacacaaat gtgaaataga
    25801 aagagaaatt aaaatggaac aaatcagagt acgaatagcc ccttcgccaa gcggaaattt
    25861 acacatcgga acagcaagaa cagccctgtt caattacctt tttgcaaaaa aacacaacgg
    25921 caaattcgta ttaagaattg aagatactga cctagagcgt tcaagcgatg aatacatcaa
    25981 aaatattttt gacagtttaa aagctttggg cttacagtgg gacgaaggtc ctgacgttgg
    26041 aggcgaatac ggcccttaca gccaatctca aaggtttgaa acttatcaaa aatacgcaca
    26101 aatcttaata gataaaggct atgcgtacga gtgtttctgc actcctgaag aattggaaga
    26161 agaaaaaaga atttctcagg agaaaaaaat cgctcatatt tattcgggca aatgcaaaaa
    26221 tttaaccgat gcacaaaaaa aagaattgag aaaagaggga agaaagcctg ctataagatt
    26281 ttctgtcgaa gcaagagaat taatttttaa tgaccttgta aaaggagaat taaaactaga
    26341 tacgggttta atcggtgatt ttgtaattat gaaatcaaac ggttctccga cttacaattt
    26401 tgcggttgta atcgatgaca tgctgatgaa aatttctcat atcataagag gcgaagacca
    26461 catttcaaac acgcccaaac aaattttaat ctacgaagcc cttggagcga aagttcctca
    26521 attcgggcac ttggggatga ttttagcacc tgacagaagc aaactttcaa aaaggcatgg
    26581 cgcaactgcg gtgagcgaat ttattgaaaa aggttatctg acagaagctc tggtaaattt
    26641 tgttgcgctt ttgggttggt cgccatcaga cggggttgaa ataaaaacag tagatgaaat
    26701 tgcggctgat ttcagaattc atgaagtttc ttcttcaaat tcgatttttg aatacgataa
    26761 attaaactgg atgaactcac aatacattaa aaaacttggg gtaaatgagc ttaaagaaaa
    26821 attgctaccc tttttaaaag actatgattt atcggaacta agcgaagaaa aattaaccag
    26881 aatgattgaa atcactcgtg aaccgcttgt tattttatcc gatataacag acgcagtttc
    26941 ttaccttttt ggccaaggag taaatgttga gcctgaggta caaagcgaag ttttggacac
    27001 cgaaatttct caaacagtat taaaagaagt tgcacaactt gctcaaaatt ggaattttga
    27061 cgatgaagaa gctcttcacc acacgcttga agaattgaga aactccttca aagaacgcgg
    27121 aattaaaccc aaagaaacca tgtgggcaat aagggctgca gttacaggca gaacaaaagg
    27181 tgccgacatt tgcgccgtgc tttggagttt aggcagggaa aaagtattaa acagaataaa
    27241 caaagcctta aaataatttt taaagcggtt taataccaag tttttcaagg ctcattttta
    27301 cgttttctga aatgattttt cgaaccaatt taaggtcttc ttttgtgtat gcagactgct
    27361 ttaaaggcgt aagtattgct cttgtcataa acttaaatga aagaatctct gcaacaatgg
    27421 cggaagtttg aaaaatgatt gtcttatcat tttcggttct tcccaaaatt gaagcaagaa
    27481 tttttctgaa agccttataa aatatttgaa ttttttcatt atacaggttg ctgaattttg
    27541 aattagaact ggtttgctct tttatcatca gaataacaaa attgtttgga acactttccg
    27601 aataaataaa agagatatat ttatcaagaa gctcaaaaag caattttatt ttttcttcgc
    27661 gtgtcatttt gccgatttca tttgattttt caatttcttc ttgtataaat ttaagcccgt
    27721 aatttaagac cgattcaatt atactgatgt aaagcccttc tttggtctta aaataataag
    27781 aaatcaaact caaattaact ccggcatgtt tgcatatttc tcttgtactt gtagagtcaa
    27841 aacccttgct tgcaaacaga attattgccg aatttataat tttttttctt gataaatttt
    27901 gtacttttct tactccagtc attttttaat gctaaaacaa aaaaaactca aaatcaattt
    27961 tgtaaaaaca ggcgtaatat ataaacaggt cgcttgcttg aaaataaatc ggtcgagtga
    28021 ccaaataatg cttgattata aacaaatgta aagctggaaa cactattgaa atttggactt
    28081 taaccctatt ttaaagttat atggagtttt tgttttaaaa atggtaagta tttgaatacg
    28141 attttaaaaa gagaatacga taaatttaaa aatatataat ttatgaaatt tttgtcgttt
    28201 ttctttaaga cgtattatta gattttggaa ttaggagtat ttcaatgaaa agattactaa
    28261 catttttact gcttggtgtg ttttgcacaa gcatgtcact accggctttg gctgctcctg
    28321 tagcactttc aggtgctgcc gcggcaaacg taaaagcaat caagtacaaa ccaaatgcaa
    28381 gaagcatcaa ctatgctttt gtctttgacg gaccttcgga caaaaacgaa gttgtactta
    28441 aacaatttga aaaagcgata acggcaacga cagcaccgga ctacaaagct gaatttccaa
    28501 aaaatttagt atttgtcgga aactgggctc aaagcggagt aaaaaccgca agcgacaaag
    28561 ctttagcatc aaatgccaca atggttgttt cgctggggta tttgtcaagc aactatcttg
    28621 cggataaaaa gggcaaaaac aaatttgtcg taacgattga ccaatacggg ctcagagata
    28681 taggtgaggg cttttttaac cccgttcagc aatccgtaaa cggtattaaa aacttcaaaa
    28741 ccttgctcag cttcaaaaaa gcagctattt taatgaacga aaattattat aaaacccaaa
    28801 aagactgggc taaatttgca ggggataaaa ttcctaacat aaattttgct gttatacctg
    28861 caacaactga tgttgcagcg acaatggcaa gtattccaag cgactgcgat gctgttgtat
    28921 taaccccctt atttaacctt tctgtagaac aaagaaaaaa tcttatttct caacttaatg
    28981 ccaaaaaaat ccctacatac tcaacactag gtaaagaaga tgttgaaatg ggggttttac
    29041 tcggaacagg cgcatacgat ttggacagaa aagtcgcaga aatgacttct tttaacatta
    29101 aaggcgtgct tgacggtcaa acaaaaatgg ctggtaaagt taatttctac gaagatgaag
    29161 tcctttatat gaatagggac actgccgatt taatcggtta tcagcctcat ttgagggttt
    29221 tatacaacgc agaagtcatt tcaaacaaaa aacctaccgt atatgattta tcaactatct
    29281 ttaatctttt agaaacacaa aacttagata tccaacgtaa gaagttactg gtaaaagcag
    29341 caaggcgcgc aactgctgca gccgctttaa gatatttacc ttctgcggtt gttaatcttg
    29401 gttttcagca atacgatgaa gattatgctt acacggtaaa acttatgcag cctgaaaaaa
    29461 caggcatttt ccaaataggc ttggaacagg tgctttattc tcctgcactg gttacaaaca
    29521 taataattaa gaaaaagggg cttaattttg caaaacatga acaatttatg gttgaacaaa
    29581 acatgggaat agatattgcc cttctatata ttgagacaaa catgctcgaa aacatgatta
    29641 aagtccaaaa agaatacgtc aaagaagcaa gagaaaacct tgcaatcgca cgtgtaagag
    29701 aaaaaatggg tgtttgcgga aatgaagaaa ccatgagatg ggcagcacag gtcaatatca
    29761 aagaacaaca ccttcttgat atgagagaag aactcaaaaa cttaaaaata gaaatcaaca
    29821 aaatgttgtt caaagaccag aaagaacagt ttgaattagc ggcattaaca gctaaagacc
    29881 ctgctttcta tacaaaagac ctgcatgtaa tcaactatgt aacaactccg gaaaaacttg
    29941 aaaaatttac tcaaatgctt ataggtgaag ctttcagggt tgcaccggaa ttggcaaaac
    30001 ttaaaaccgc aatagaaatg aaaaacgcgg aagcggctat gtattatcaa aaattcatat
    30061 tgcccgatgc aaaattgact tatacttata catcgcttat ggacaggcaa ttcgcagcag
    30121 gactgcctag tgccccattt gcttattcac cacctgcagg cggcaccatt ccgggttcta
    30181 tgtggaccaa cttaagtgct ctgcgcccag gtgcaacaaa ctctcagttg ggaatattcg
    30241 cccaatggag accgattgaa ggcggtacta aaattgcaga aatagcaaga ataaatgctg
    30301 aaagacaaga acttcaaaga tatcaggatg aagttaaaac cgctatcgaa gaacaaatca
    30361 gacaaacaat caacagagca gtagcagcat acttcagtat agagaaaaac tataaagcaa
    30421 tgtatgcttc tcaagaaaac tacgctaaag taaaagaaag atacttaacg ggtaaagctc
    30481 ctattgacca gttaatcgat gctgaaacca cttacctgaa ctctaaagaa aaagcaatga
    30541 attctcaata cgttttcttt aaagaacttg tttgggtaca aagagcaata tgtgccgtaa
    30601 actgggctac agcatcaact gaggcaaaac agtttattga aagcatcaaa aaggaaatta
    30661 cacctcaacc cgatgtttca ttgctgtaat aatattaatg cataattaaa aggcgaagcc
    30721 gcaaaatgtg gcttcgcctt ttaaaattca gaaaagaatt aatttattct tcaataaaaa
    30781 atctttcatc cgagagaata tttttcatat tcaaaatttt ataaagcttg tcgtctataa
    30841 caacctcgct gaggatatat ttattcatcc tgttgtaaga gttttgaata attagttctt
    30901 ctgcaatatt cattatatca aagatttcat ctgttaaaaa acctatttta aaatcgactg
    30961 tttcaagtat tataacccca ttggattttt gcaaagattt tttataaggt attccgagaa
    31021 aatttttaac atcaacaaca gtaataaaat cacctctgag agttataatg cctgctatat
    31081 aatcgggcgt acaagggacg gttgtaatag aaacgtcttt aagaaattct tttacgtatt
    31141 caagacttat acaatatacg ttttgaccta gaagaaaaga aatgaattcg tcttgggaga
    31201 aaatattttt aaccaatcct tgttttgatt tttcacttaa ttcggtattc ctttgcatta
    31261 atttatatct ggaatcatcg tcgttgggaa acaaagatga gatattgata ttttcatcct
    31321 taaccccttt ttttaaaata ttttccaggg catttaagtt aataaccgaa attatttcac
    31381 ctttatttct gtaagaaaaa tccactattt tttctttttt tatttgagaa ttatattcca
    31441 agcttgaatt ttcgattgaa acaatatctt cgactttgtc tgctataagt ccgaacatcg
    31501 actcgtctgt ttttaccacc aaaagctggt ttgaaagcga ataaggagtt acttttacat
    31561 taagataaaa tcttaaatca agaacattca aggtgaagtt attatatcct aaaagcccta
    31621 taacattatt tttaagcttt tggggatact ccaaatacgg aagtttcatt atctcaacga
    31681 cttgggaaac ttcaacggca tattttttct ttccaagtga gaaataaaga tattgtgttt
    31741 cgtctttgtc aacgatctga ttattttgaa atttctgcaa attgttcata agagtatatt
    31801 acaaccctgt ttcttccgtc ttgctttgcc ttgtaaagtg ctttatcggc actttcaaca
    31861 aaatcttttt ctgtttcaat tgtttcaaaa ttagtactca aacctatact aaccgtaata
    31921 tttgtttcaa tatcattata gcaaaatttg tactgagaaa ttttttccct aagcctttcg
    31981 ataggaataa gcgcattttc aatagttgtt tcggtaagga ttatcgccat ttcttctcca
    32041 ccgtagcgat aaatcatatc ggttttcctg aaagaagcgg ataaaatatc tgatatttct
    32101 cttaaaacat aatctccgta ctggtgtccg taggtatcat taactttttt aaagaaatca
    32161 atatcaatga ttgccaaact caaatcgcct ttataacgca ttacccttga aaattcacgc
    32221 tcaaggttgt attcaaaatg ccttctgtta taaagtccgg tgagtccgtc tgtaactgaa
    32281 agatattcgg tttcggaata taaaaatctc attttaaata agagtaaaaa ttcgcttacc
    32341 attgtattgt aaaatttaaa atcagaataa tttatttgat tattactgaa aaaacaaacc
    32401 ccgcccaaaa gcttatcttc aaaaattata ggaaaaacat ggcttgattt aatatcatca
    32461 aaagatacta ttttttcttc ttcgttaatt ttttctctaa ctatgtcatg cccgaaatct
    32521 cttattgtaa attctggcat atcaggcata tttgaaaaaa agtcacgctt aattttttct
    32581 ataacaaagt ttgatacaaa agatttattt atatcaagat gaaggatatt tttgtcattt
    32641 ttatcaagcg atttaaaaaa tattcccgca acattatagt caataaacat gctcaaaagt
    32701 tcaaaagttt tttcgattaa aactttttcg tgtgtcaaat attcacctaa atccctgaat
    32761 tcatttaaaa aagttgaatt cataagcaat tcgtcaagca catcgtttat ttgattttga
    32821 acgctttctt cgcgaatttg ctgatttaaa ataaattttt tgtagtcctc agaaactctt
    32881 ttttgttcaa gaagctcgca tactttaatg tgcaaatcct caaagttttc gtttttagac
    32941 ataaatgcat ccgcaccgga ttttccagcc caaaattcgt ctattttttt atccaaaact
    33001 gtgaacaaga ctataggaat tttttttgtt gcaggagtgt ttttaagaag cctgcaaagc
    33061 tgatacccgt taagatttgg cattattacg tcagaaagga ttatatccgg agcgttttca
    33121 aaaacctttt tgtacccgtc agcaccgtct tgtgccgttt taacctcaaa gcctgctttt
    33181 gtaaattgaa ttttcaatga ttcaagctgg gtggtgctgt catctattaa taagattttg
    33241 tttgccataa agtatctttc gtatttataa tagaataatt atacaacaaa ttaatgatta
    33301 ttaaaataag ttttacattt ataatataaa ttacataatg taaaataaaa aggagcttat
    33361 atgaaaacag attcaaaatt taatttggga caaagaatta aaattaaaaa tataatcgat
    33421 atttcacttt tacttttaat gataacggct gtccttcttt cgggattttc aatgaaaata
    33481 ggacgctacg acgcgcttat tatgtctttg gtaattattt tttacattgc gcagtatata
    33541 atgagtcaaa actggattaa taatgcattc aaaatcagtg ttaaaaaaca gataaaccaa
    33601 acaaccgcgg ccactgaaga aatttttgat tcggttaaaa aacaaaaaaa ctctttactg
    33661 gaacatttag aatcagttaa aaccgccttg gaaaatataa aagaacttaa agctaatttc
    33721 gaaaaagcaa aacaaagctc aagcaatgtt tcttcaaagg cagaaaaatc tttagatttt
    33781 tctcaaaaag aacaagaagc cattaaggca aatatcgaaa aaatgataac actaaggcaa
    33841 aaaattcaaa taatcgccga attgattttg gaactttcag agcacaccca acaaatagga
    33901 agcattatag gaatagttga agacatcgca gaacagacca acatgcttgc cctgaatgct
    33961 gctgtagaag ctgcaagagc aggagaacac ggaaaaggtt ttgctgttgt tgcaggtgaa
    34021 ataagaaaac ttgccgatga atcaaaacaa gctacaacaa aaatcaccaa cttaataaaa
    34081 gacattcaac aggcaacaaa ttcaaccgtt atggcaacag aagaaggctc aaaagaaatt
    34141 gaactgggag ttaagcttgc cgataatata aaccaaaatg ttgacctgct aaccggactt
    34201 ataaaagaag ttaatttatc tgcagaagaa atttgttcaa attccaaaat tcaaacaaat
    34261 tattccgaaa aagcacaaga ggctattgaa aatgtttgcg aagggataaa aacatctttg
    34321 gactctttag aagaaaatat ccaaaaaata caagctttaa attttatttc tgtttctttt
    34381 aaggaaaatt ttactgacga acaggatttt tagatggatt ttcaggaaga agaatttaac
    34441 gagattttaa gaatctttca aattgagtct gaagaaatta tctccagact caacaacaat
    34501 attcttgaac ttgaaaaaac tccggacaac aaagatatta ttgttatcct attcagagat
    34561 gcccacagct taaaaggtgc tgcccgaatg gtcgggttta atcatattca aactttagct
    34621 cacaaaatcg aagatatctt gggtcttgca aaagataatc aaataagact tagtcctgaa
    34681 acagtaaacg ctctatataa aagcgtggac tttttgtctg aaataatagg aaaatctatt
    34741 gagcaaaaaa ctgaatttta tgatgaaaac ttggctgtcc aaataagtct tttggataaa
    34801 ataagtgcgc agcctcaaga aaataccgaa aaatcaaagg aagaatttag cataaaagaa
    34861 cttaaaaaca atattaatga aataaattct ttaatccccg aaggtctttt tgaacttatg
    34921 agtgccttaa gtggacaaaa tcaggagata ataaaagaat tttcacaaaa aattgacagg
    34981 ctttttgaaa tttttaaaca agtgggaata tacgatatta aaaaatccct tgaagatata
    35041 aaggtaaaat tggattttgt aataaaaaca tcaggaagtt tcaacttaaa agaaattgat
    35101 gattttcaaa aaattatcga cggagttatt tcaaatcttg tttctgtttg cgaaatacac
    35161 gcaataaaaa cccccgatta ttattcgctg gcatttgaaa tgttttcggc aaaatctgac
    35221 aggtcttctg ctcaaaaaga gcccgacatg cctgaaaaaa ctttagaaca ccaagaggaa
    35281 atttttgatt taccccctga agccattgtg caaaaacaag aagtaaaaga atttgataat
    35341 aaaaatcttt taaatataaa agaaatgttt tcttcgctgg gtcaaaatta tacttcttta
    35401 ggagaattaa agcaaaaact tttagatttt gaaacaacgg caaaagatga aattataagc
    35461 cctataataa ataatacctt aaaaataatt gatttttcaa taaaaaacaa aatgaaactc
    35521 aacgaagatg caatttctgc gatggaacaa ggcattgatt actgcctttc aagattgaac
    35581 aatcaagaag aagcgaccga cagtgccctt gttattcaaa ggcttgaaat tattcagcaa
    35641 gttttggagt taagcaacca atcgacggaa aacgaaaata ttttaccgcg aaaaagtacc
    35701 gatttaatta aaaagaattc tgatttgaat gcaatttttg acacaggaga aattaaaact
    35761 ttaagagtag attctataaa attggattct atggtaagtc aagtcggaga gttaatgatt
    35821 gcaaaaataa aggcaaaaaa acatatctac gggcttgatt caataaatat atcaatggac
    35881 gaatggcata aagactgtgc aaaagtttta aaccacctta aatattttga aaaaaaatac
    35941 cctaaattgc aaaacgaaga ttctcaatct ttttacttta taaaccaaat actaaatgtt
    36001 cttgaaaaca actgcagaaa attttcagat accctcaaag aaatttcaaa tctgcgaaga
    36061 atgatacaag aagacgaaac aaaaataagc cttataatag acgagttgga aagcaccgtt
    36121 aaaaatataa gggttttgcc ttttgcaaca attttccata ttttcggcag aatggtaaga
    36181 gatatagcac aagaaaaagg taaacaaata aactttgaaa tatcaggaag cgaaacaagc
    36241 accgataaaa aaatacttga agaaattaaa gctccgctca ttcacattat aagaaattca
    36301 atagatcatg gaattgaaac cccacagcaa agaatttcgg cagggaaaga cccggtgggt
    36361 aaaataatcc ttaaagcttc tcaaacggac aataaagtaa aaatagaaat ccacgatgac
    36421 ggcaaaggaa tagacgttgc aaaaattaaa gaaaaagcct taaaaaaagg ttatttaaaa
    36481 aaagaagaag cagattcgat gtcagacgaa caaataacca acctgatttt tgcccccgga
    36541 ttttcaaccg gagatgaaat tacaagtatt tccggcagag ggatagggct tgatgttgtt
    36601 cagactaaaa tttctcaact taacggaaaa gtgaaagtcc tttccgaatt aaacaaaggc
    36661 tgcagtgtga taatagagct tccgacaaca atgtccacgc ttaagtcttt tttggtaagg
    36721 gcttcgggac aaatttttgc tattcctatg agtgttataa attcggttat gctcaaaaac
    36781 tcaaacgaaa tattttttaa cgaagggcta aaaacaatca ttatagaagg caaaaccgtt
    36841 cctatataca atttgtcagc aattttaaat ttacctttac atgagcaaaa cagcaataaa
    36901 aaaaccattt taacattgga aagcgataat aaaataatag ggcttgaagt tgacaaactt
    36961 ttgggggatc aagaaatttt acaaaaaaaa ctttgcgcac cgctttataa gctcaaaaat
    37021 atatccggtg ttactactct tgcctcgggt gatttatgct ttatcttaaa tatttccaaa
    37081 attttgaaaa atataaacaa aataaattac atagagcaat ctcagcctta tgaaaaaatt
    37141 tcggttaaag aaaataaaat cctgttggtt gatgattcta taaccacaag aactctggaa
    37201 aaaaatattc ttacaaaagc cggctacagc gttgaacttg cactaaatcc gatagaagct
    37261 cttgaaaaac tctcaaaaga aaagtttaat cttataataa gcgacattga aatgcctgaa
    37321 atgaacgggt ttgaattttt gcgcaaaata aaaagcgata aaaattactc tgatattcct
    37381 gttcttatgc taagctctct ttataacgaa caagaacgca aaaaagcaaa agactacggc
    37441 gcaaaagaat acttaaataa aaatgagttt aaccaagaag agtttttagg cataataaac
    37501 aatatttttc aataaataat tatttaaaca aattcagctg tggggcttta atttgtagtt
    37561 gattttgttc atctttgctt gaacgttttc gggcggataa atcatttgaa aaatcccgct
    37621 gcattttttt catcaaatcc tgtgaacggt ttattacact ttgcggaagt cctgccattt
    37681 tagccacttg aattccgtaa cttttgctgg caccgccctg tacgattttt ctcaaaaatt
    37741 cgatttcgcc gttttcttcg cttattgtta ttctgtaatt ttttatctgc gggtaagtgt
    37801 ttgtcataac atttagttcg tggtaatggg ttgcaaaaat acaccttgcc ttaatttttg
    37861 tcgctataaa ttctgccaca gcccaggcaa tagcaactcc gtcataggtg cttgtgcctc
    37921 tgccgatttc atccaataga atcaaacttt tttcgctggc agaattcagg atgtagcctg
    37981 tttcaatcat ttccaccata aaagtcgatt gtcccaaagt taaatcatcc gatgcgccca
    38041 cacgggtgaa aattttatcc acaaccccta atttgacgta atctgcggga acaaaagagc
    38101 ccatttgcgc caaaatcgct atcagcgcat tttggcgcat aaatgttgac ttacctgcca
    38161 tgttaggacc tgtaagtatc ataaattggg ttgaggagtg agaagaaagc ccctcgcccc
    38221 ttgagggaga gggtagaatt tcggcacgag agctttgctc aagtgcttga aattcgggtg
    38281 aggggtattt ttgggttgct tcgtcgttcg cttcgctcac ttctcgcaat gacagatctg
    38341 tttcttgttt accatttacc gtttcctttt gataagccag ttccaaatcg tttgccacat
    38401 aacttccaag cggcaaaact ttttctaaaa cggcatggcg accgtttttt acaagaaaat
    38461 ccctgctttc atctaccgtc ggtcttgtat aattgttttc gacggcaaca aaggcaagcg
    38521 aagttaaaac atcgatattt gcaatacaat ctgcgacttg tcttattttt gtaacgtatt
    38581 cttttgcata atttttaaaa tcgcaaaaca gtttattttc aagttccaat gccttaaatt
    38641 gtgccgaaag cacgtcatct tcatgttttt tcaactcgtc tgtgataaac ctttcggcat
    38701 ttgtaagggt ttgttttctg atatagttat tgggaacaag gtttaagttt gaatttgtga
    38761 tttcgataaa aaagccaaaa actttgttgt agccgacttt caaaaacttc attccggtgc
    38821 gctctttttc tttttcttca aattctttaa gccatttttc gccgcctgtg agcaaatccc
    38881 tgaaataatc gagttcgccg cttacgccgg attttataac tccgccgtct tttaccgaaa
    38941 taggcgcatc ttctcggatt gtacgctcaa ttatgtttgc aaattcaatc aattcttgtt
    39001 cgtattgact taatttatca aaagagtcgc tttcaagccc ctgtatttct tttattatct
    39061 gaggaagcat aaaaagagag tctttaagcc cgagaaaatc tctgggattt gccgaattgt
    39121 tgctcaatct tgtagaaagc ctttgaatat cataaatctt ttccaaaatt tgtgccaaat
    39181 tgcctcttgt ttgaggtttt tggattaact cttctataat atcgagtctt ttgtttattt
    39241 catcaacatt tttcaaaggt tggcaaatgc ggttttttaa aagccttgcg cccatattcg
    39301 tctttgtttt atcgattgcc caaagaagcg aaccgtattt gttttgtcct cgaagcgttt
    39361 gcgtcagttc aagatttttt cttgtattgg catcaattac tacaaattcg ctcaattcat
    39421 agggcgttat tgtgtcaaat ttgggaaaat tttctttcat cgtttcccaa atgtaggaaa
    39481 caagagcccc acatgccaaa aaccccgttt tataattttc ataaccgaag gcctcaagag
    39541 aatttgtatt aaaaacggtt tttaaattat tcagtgcaaa attctgctca aaaatgctcg
    39601 aagggacttt cgaacagttg taattattta cgatttcatc gggcaggtcg attttttcat
    39661 cgggcacaat ctgaaagggc atgacttttt gggcgattga aggtgctatg atttcagagg
    39721 gttgaagcct tgcaagctct gataaaatca tactcaaagg ggcatgcgtt gtcttaaatt
    39781 ctcccgttga aatatcggca taagcaaaac cgaacaggtt atcatctctt ttatctttta
    39841 taattgcgca gatgtagttg tttgaatttt tatccaacag gttgctttct gtaagcgttc
    39901 ctgcggtaac aagccttacc acccctcttt ttacaagcgt ttttgtctct ttggggtctt
    39961 ctaactgttc gcagatagcg actttgtaat ttttttcaat taatttttca agataatttt
    40021 ctactgcttt tgcaggaact cccgcaagcg ggattctgcc catttttgcg ccggcatctt
    40081 tgcccgtcag ggtaagctcc aattcccttg acatgatgat tgcgtcttca aaaaaggttt
    40141 cgtaaaaatc cccgaggcga tacaacaaaa taacatcctg atgctgtctt tttatctcaa
    40201 gatactgggc aataacaggt gttacgtcct caatatttat atctaaactg tttacatgat
    40261 tgatttcttg ctttgacatt gctacactta tcctagaaaa acattgggaa aactcccaaa
    40321 ttatacattt tgtcatgctg aatttatttc agcatcttac cagcaaagta cgttggatag
    40381 atcctgaaac aagttcagga tgacaggcag atttagttaa attatcttat catgaaatca
    40441 ggagaaattc atgggctgtt taaaaaatat ttttaaagca ataattcttg ttttagctgc
    40501 tatcggattt gtgtctatag gcggcaaaga ctacgtcata aacctttgga atcaatacac
    40561 aaatccgcct caagatgtca tgctcgagcg tgccaaaaaa gttggggatt tctcccaagt
    40621 gcccgatgaa tacgaaatag ataaagcagc atcggttcta ggctataatg gagttttagc
    40681 agagcataga gcaacgggac agaaaatggt agtggtggat tcgggcaaaa aacccctttt
    40741 gacacctcag gattttgaag atggaagtgc cgataaaaaa ttggaagatt taacaaaaaa
    40801 attaaaatac cagtttataa acatacagga tttaaagatt acaaaacacg gtaaaattaa
    40861 ttctttcggg caaaagacaa atttcataaa atttcaagca aaagcaaata aactgcccat
    40921 aggagaaatt gagggcataa tttctgccgt tgaaaccgaa gacggaaaaa ttaaaataat
    40981 cgcttctgca aacgaaaaag gcaaatattc ccaaatagtc tccgaagaat ttttcaaaaa
    41041 ggtaaaagaa tagtttataa gtttataagc tgccaagccg ctaaacttct tagtcactca
    41101 gttacttagt catctagtca cttttactat ttctttacaa atatttgttt ttaaattaaa
    41161 ataaatcttg ttaaatctgt tatgtattga aatttaaaag agagtcgcgg tgtctgatgg
    41221 gaaaagattt aaaatacaaa agagttttgc taaagattag cggagaagca cttttgggct
    41281 cgcaggaatt cgggattgac cctgttccgg ttgaaatgat agccaatgaa attaaactcg
    41341 cgcacgacga aggtgcgcaa ataggcgttg tagttggcgg cggaaatatt ttcaggggag
    41401 ttaaaaacag tgccaagctt ggaatggacc aagcatctgg cgattatgtc ggaatgcttg
    41461 caaccgtaat gaatgcgctt actcttcaaa gcgcgcttaa aaaagtcgga gtttcatgca
    41521 gggttcaatc tgccattcag atgaatcaga ttgcagagcc ttatattcgc tttaaggcta
    41581 taagacacct tgaaaaaaac agggtggtga tttttgccgc aggtaccgga aatcctttct
    41641 ttacaacaga tacggcagca tctttaagag catcagaaat taacgcagat gccatgctta
    41701 tggcaaaaaa cggggttgac ggagtttatt cggatgatcc cagggtaaac aaaaaagcca
    41761 aaaaattcga taaaatatct tacgatgata ttataaaaaa cggtttaaaa gttatggata
    41821 ctgcctcttg tgcgctttgc aaacaaaaca atatccccat tatcgtattt gattttcagg
    41881 caaaaggcag catcaaaaag attttacacg gtgaaaattt aggaacttat gtaggaggat
    41941 aatatgtcag aagcagtaga tgaaatgttt ttattcggcg aagaaaaaat ggaaaaggca
    42001 cttaaccagc ttaaaaagga atttgcttca atccgctcag gcagggcaaa tccgatgatt
    42061 ttagataagg ttatggtaga ttactatggt gctcctactc cattaagaca aatgtcgcaa
    42121 gtaactgttc aagacggaac aaccattgtt gtttcgcctt atgataagtc gataatcaaa
    42181 gaaatcgaaa aagcaataat aaaagccgaa ttaggaattc ctccaaacag cgacgggatt
    42241 gtcataagac ttgctttccc tcctttaact gaagaaagaa gaaaagaaat tacaaaagat
    42301 gttaaaaaac taggcgaaga ttcaaaggtt gtaataagaa acataagaag agacatggtt
    42361 gacgaattaa aaaaactcga aaaatctgaa aatcttcccg aagatgttgc aaaagacaat
    42421 caagatgaaa tccaaaaatt aaccgacaaa tacgttaaaa tgattgatat gaccgtagaa
    42481 gaaaaagaaa aagaggtctt aacagtttaa tgaatttaac tttaaacaaa aatgcaaaaa
    42541 gaattattac aggctcaatt atggggctta ttgccctttg gtgcctgatt tatggaggaa
    42601 tcgcccttct tgctttgatt actattgttg tgtttttggg ctcaaaagaa tacgttttaa
    42661 ttttggaaaa taaaggattt ttcccgagtt taaaagtaat ccttttttca gatgccgttt
    42721 ttgcgcttct ttgttatttt aaccgctttg atttggttgc aatcgcattt acaatatgtt
    42781 ctattaccgc atttttgtgg gtattattta aaggcaggca gccttatatc gcaaatgttt
    42841 cgaccacaat tttgggtttt gtctatggcg ggtggttccc gcttcacctg cttttggtaa
    42901 ggggacttaa cgcagaaaat tatcatgggt ttttaaaact taatattcaa aatgacggac
    42961 ttggatatgt tatgttttta tttacggtaa ttttattcac agatacaggc tgttattatt
    43021 tcggaaaaaa attcggaaaa cataagcttg cagctgtcat aagccctaat aaaaccatag
    43081 aaggagctgt gggaggaact atatgtgccg taatcagtgc ttttatcgtt gggcattata
    43141 ttcaccttgc atggtatcat tgcttggtta tggggctttt atgtacaact tttgcacaac
    43201 ttggcgattt atcagaatct ctgattaaaa gagacgccgg agtaaaagat tcgggcaata
    43261 cgcttcccgg acatggcgga tttttggaca gaacagacag ctacgttttg actattcctg
    43321 ttatgtttta ttatttaaag tattttgtcg tttctaacca gctttggttt gattttgtta
    43381 actttataaa aggatttttg catgcactcg gagtgtgaaa atttaaaaaa attggaaaag
    43441 atttttaaaa ttaagctgga aaatcttaaa atttttgaaa aagcacttac ccacagttct
    43501 tttactaaag aaaatgatat ttcttccttg gaaaattttg agcagcttga atttctggga
    43561 gatgcggtct taaaattgtg tgtttctgat attttataca aaaagttccc aaaatattca
    43621 gaaggtgaac tgacaaaaat ccgctcaatt ttagtttcag acaacaccct tgcaaaaatc
    43681 gcaaaaaaaa taaaactcga caaactctta atcctaggaa aaagagaaga aaaatcaggc
    43741 ggaagacaaa aaaattcaat tcttgcctgt gcctttgaag cggttttggg agcatatttt
    43801 ttagaaggca aatacattga cctttcaaaa aatttagaaa aaatactaaa accttttata
    43861 aaagaaattg acgaaaattt tgaaaaattt aatgcaaaag caattttgca agaatatacc
    43921 caatcgcaaa acaaacaaac tcccttatat aaaatcgttg aagaaaaagg tcctcaacat
    43981 gacaaagttt ttgtgataga agtatcttat atggaaaaag ttttggcaaa aggcgagggc
    44041 aaaacaaaaa aagaagccga gcaaaatgct gccatgctgg catgtaaaaa actcggagta
    44101 ataaacagtg aacagtgaag cgtgagcagt gagcagaaat gtcctttcac agttcaccgc
    44161 tcactaaaaa catttcacac caaggaggaa aatttattga aaactattat ttcaccttcg
    44221 atattatcgg caaattttgc aaatcttgaa cacgacatca aagcagtcga aaaagcaggg
    44281 gcgcattggc tccacattga tgtcatggac ggacattttg ttcccaacat cacaatcggc
    44341 gttccggttg ttaaatcaat taaaaaaata acttcacttc ctttggatgt tcatttgatg
    44401 attgaaaacc ccaaaaagtt tgttaaatct tttgtggaag caggagctga cattataacg
    44461 ttccactatg aagcctgcga agataaaatt ttagaggtaa tctattacat aaaatctttc
    44521 gggataaaag cggggctttc cataaaacca aagaccgatg ccaaagaaat aattcctttc
    44581 ttgtccgatt tggatatggt tttagttatg actgtagagc ccggatttgg cgggcaggaa
    44641 tttatgcacg attgcgccca aaaaattcct ctgataaaac aaaattcgcc tgataacatg
    44701 ataattcagg tggatggcgg cataaataac gtaaccggaa aaatctgcac ttcactcggc
    44761 gcaaattcgc ttgtggcagg aaattatatt ttcaaaagta aagatataga acaagctgtt
    44821 aaatctttga tgtaataaaa agcataaaaa caggggcggg gttttttaaa aaaccccgcc
    44881 cctgttttat atatttttta tacttttata ttaagtattc tgccttctgc ctgataacgg
    44941 acaaaatctt caggtgctaa aacatttcta gtaacctctt gtgttcttat tccgccttcg
    45001 taagggagat aagtttctcc tattactttt tgcaccaaag ggcttttaac ttttggttga
    45061 acggcatgaa ttataacctc attgactttt gtaaaagtat ccgcgggtga tgtcgtaagt
    45121 tcttccaaaa gtgctttttc ttgcgctttt gtcattggtg gttttttaat actttctgcc
    45181 gcatcaacaa caaactttct gactccgttt tcaaatggta taaatataat ttttttaact
    45241 gcacttactc ctgtcatagt cgcctctttc ataaaatgta ataacataca tcaatattaa
    45301 acatcgtcaa aaaatttttt gctagttttt taagaaatat taattttttt taacattttt
    45361 tcaagtccgg caagtttttt tgcacttgta ccaagcccgg tcaaaaacat aacggacttt
    45421 tggtttgaaa tttcatcttc aattttgtat ttttcaaaca ggatttttga aagctcaaaa
    45481 ccgcttaatt cagggtgttt tgtcagtatt tttgtagaat cccctttaaa aaattctaaa
    45541 tttggaagat tttttttcat attttcaata ttttcaatca attccaaaat ctttgccctg
    45601 cctttttttg aatttaaaaa gttaatgttt ttttctatac ttgcaagcat tggataagaa
    45661 gggcttgtgg ttgaaattaa agcaagtgcg ctttgaatat cgatatcttt taaattgcag
    45721 tgcaaaagtg cagtagggtt taagccgcct gccgttttgt gaagtgagtg gattacaaaa
    45781 tcagcccctt gataaatagc agattttgga agcttatcgc aaaaaggata cagagccccg
    45841 tgtgcttcat ctactataag ataagtttta tattttttgc aaagttttga aattttttca
    45901 acatcacaaa ccactccttc ataagtgggc gaagttatga ttagtgcttt aattttttct
    45961 ttttttaaaa tctcttctat ttgatttaat ttggggattg tataaactcc ccaatctttg
    46021 tcaaattcat actcgtaaaa aacaggctgc gcaccgacaa gaataaccga actattgtgg
    46081 cacctgtggg ctttatccca gattaagaat ttttcacccc tcaccccgac cctctcccgc
    46141 aaggggcgag ggggcaccct taaatcctca tgctttttcg tacatgctaa aaccgccgca
    46201 ataataccgg atgtagagcc gtttgttaga aaatgtgtcg attgggtttt ataaattttg
    46261 gcggcacttt tttgcgcgca aaaaagtgcc gcttcaggat tttgagcctc ggtttctgaa
    46321 atatcgtatt tataaaaatt tctcaatttt gaaaaaatga aaaaactttg tgaatgagat
    46381 ggtgtagtaa aacattcaac ctgatttttt ttctttaaaa tttccaaaaa ccccatgact
    46441 tcaactttca gctaatcatt tgcttcgtct agatatttaa ttgcctcggg aataacttca
    46501 agaaaaacat cggcaacatc aggatcaaat tgtcttcctt tttcaagctc aattcttttt
    46561 attgcctcat cgtgcgaaag cccttgccta tatgagcgtt gagaaaccat actgtcataa
    46621 gcatctgcaa cagcaataat tcttgcgctt ataggaattt catctccttt aattcctaaa
    46681 gggtaaccgc ttccgtcata tttttcatgg tgtgttttta ttatttcgat aacttctttt
    46741 aactgtttta tatcttctaa aattctaact ccgtattcca cgtgtttttt aatcatttca
    46801 tattcataag gcgtcagggc ttcaattttg ttaagaattt cttcggaaac tcctatcatt
    46861 cctatatcat ggagcaaacc ggcaagttca agttttccga tttcttcagg cggaaaattc
    46921 atcttttttg caacaagcat ggataacata gttaccctgc ggcttcttcc caaggtaaaa
    46981 gaatcttttg catcaagagc ctcaataata gcatttaccg taccggaaaa taattttttt
    47041 aaatcgtata ttaatctgtc attttcttgc tttaattttg aaaaatcaac tgctgtttgc
    47101 attaccataa tcaattctgc aggctcccag ggttttttca gatatctgaa aattttgcct
    47161 tcattgatag catcaattaa tatgttcgca tcagaataag cggttacaag caaccttacg
    47221 ctttggggac ttaattcaca gcttcttttt aaaaactcga ccccgtccat ttcaggcatc
    47281 ttgtggtcag ataaaattat atcaaaatat tttgtatcaa gcatttcaag tgcttccagc
    47341 ggggaggttg ttttttcaac tagatagtca tgcctgagtg ttctgtacaa taactccagg
    47401 ttatcttttt catcgtccac aagtaaaact ttaatttttt ccataactca attctctttt
    47461 tttgtctaaa acaacgggta actttattct aaaaaggctt cctttaccaa gttgactgtg
    47521 tgcgctaatt tgcccgccat gattttgtat aaccttataa ctgatagaca tgcccaaacc
    47581 tgtaccctct ccttgaggtt ttgttgtata aaaagggtca aaaatctttt tcaactgatc
    47641 ttcggacatg cccgagcctg tatcttcgat ttctattata acattatctt gttccttttt
    47701 taaccttatg tatattttac cttttttttc tattgcataa caggcattgt ccaaaatatt
    47761 cataaaaact tgatttaact gtccgccgta gccttctata ataggaaaat cttgatcata
    47821 ctcttttata acttctgctt tgtattttat tttattatgc aaaatattca aaatagtttc
    47881 aatttctcgt tttaaatcaa tttcatttac taccaattct tctattcttg aaaagttttt
    47941 taaatccaaa attatatttt ttgttctttc cgttccctct tgacagcttt gtataagcat
    48001 cggcaaatct tcttttataa aagagtattc cacctcttgt ttaatcttgt ttaaagtatc
    48061 ccttttttcc tcactcaaat cattttcgta attttgataa acttctataa gttcaattaa
    48121 agctttagaa taatttttca agtgcgtcat attcccgtga ataaagttta tggggttgtt
    48181 tatttcatgt gtaataccgg cgacaagctc tcccaaagaa cgcatttttt cgctgtgcac
    48241 cattattatt tgcgtatttt caagttcttc ataggcaagt tttaactctt ttgttctttc
    48301 ctgaaccttt ttttccaaat tgtcatacaa agattgaaga ttttccgcca tttgattgta
    48361 agtttggaca agctgattta tttcggtgta tttggtaaat tcaaccctgt acttaaggtt
    48421 tccctcggat aattcttttg tcccgttaat aagtttttct aaagggctta tgattatttt
    48481 tgtcatgtaa taagataaaa taagaccaaa aattaaaaac gctattacta ttctttgaag
    48541 gttttctctg aaagtaagaa ttgtcgaata cattgaatct tgagggacaa aaaaactgaa
    48601 aatactgtca acaaaaaaaa cggcataaac cgccattccg attgtcgcaa cggcaaccaa
    48661 caaactcatg cttaaagcaa aggtagtccc tatacttaat ttttctctat ttgatttttt
    48721 tttgccgata tttttcatta tcacttccga atttttgaaa tcattccata acatccgaat
    48781 caaaagacat ttcttcttgt tcgtcatttt ctttttttgt aacttctttt atgaaaagac
    48841 tattatggaa aaccggtctt cttcttctgg gtttatccag tatagaaccc atcatccagc
    48901 cgataaaccc cattgcaaag cttgcaggta taactttttt taaggtcgca aaaactgaat
    48961 aataatccag tgacatactt cctgttaagg ataatgctcc tgctataagt attgtccagc
    49021 ttgcaaatac tgcagctaat ttatgtttgt ttttaagttg atttttgctc atgatttttt
    49081 atttactgtt tccaaatttt tcttgataca aagctgtacg agagtcattt ttgccacatt
    49141 tgacatattt ttaaatcttc ccgaaaccgt ataaattcca tcatcgcttt tttctattct
    49201 tatgggctca aacaaacatc ttatttcgtt atccccaaaa agctcgattg aagtcatata
    49261 ttcagtatca atatccaagc tgtctttact ttgaattttc attccgcccg cggataaatc
    49321 caatgaattt accttaataa ccttattatt tgaatgaagt gccatttcat taataaattt
    49381 aatccttgta aactgccttc tttgcagata tctgtgtttt acgggatttt ctatttttaa
    49441 tattttgccc tccacgtcta aaacttttgc ttcaaaatat aaaattccgt tttccgtaga
    49501 agaataaaat tcactttttt tgcctttggg aatattttgc ggctcaaaat ttaattccac
    49561 agaaaatcct ttttctttca tatcgaccac cttgcctctt gtagcgtctt taaaatcttg
    49621 aggtacgata tttatagcct gctctttttt taacaatcca atcattttaa atcctttcta
    49681 ggctaccgcc tgtgaaaccg ccttaattat acccacggat tttgctttaa cattggaact
    49741 tatttcatta tacgacatta ccgtaatttg cggataagtt ctttctataa gccgcctgaa
    49801 agcaagtctt attgcagatg aacaaagtat aacaggttga ttgccgcttg acaagatagc
    49861 cttttccaac tcggagttaa gagtatctaa aagtcttctt gtaaaatcag gatcaagagt
    49921 taaactttga ccgtcagggc tgactccttg cgctatagtg ttttcaacat caggtgcaag
    49981 tgttattgca aataattctc ccgtgtcaga aagattttgt ttacaaattt gtcttgcaag
    50041 tgcctgtctt gtctgctcgg taagaaaatc gtaattttta cttatttttg cctgaaggct
    50101 tactgtttcc aaaattgttt ttaaatctct tacagaaacc ctttctctta aaagattttg
    50161 cataacctgc tgaacatctg cggcggttaa atctttcatc aaatccttaa cataatcttc
    50221 ggaaacttct ttttgtaaat tgtctatcaa ctgctgaaca tcggcacgcg aaagaatgtc
    50281 ggcagaattt ttctttataa tctcggttaa atgtgttgaa ataacagcag aaggacttac
    50341 aacggtgtag ccgtaagttt ctgcatagtc tttgtctttt tcttcaatcc aaagagcggg
    50401 aagcccgaaa gcaggctcaa tggcatttat gcccgtcaaa ttgctgtcgt tatttacccc
    50461 tgaagcattc atcgcaaggc ttctttcggg ataaacctcc cctgattcta ttgcaattcc
    50521 cttaagtttt atttgataag tattgggtga aagctgcaaa ttgtctctaa cccttactga
    50581 aggtaaaact attcccaaat ctaaagcggt ttgccttctt atttgggcaa ttctttcaag
    50641 caaatcccca ccttgctcta tattaagaag cggcacgagt ctgtatccga cttctatttc
    50701 gattgtttcg accgcaagaa gttccaaaac gctttctctt gtggcttttt tctttctttt
    50761 acccagccgc tcctgctttt gaacttcttc tttttgttta agctcggctt ctgcaacctt
    50821 tttattatcc tgaattttgt aatatgcaaa tattgccgta atcataccaa tacttaaaaa
    50881 aggtattgta ggcattcccg gaatcatacc catgaaacac aaaagtcctg cgacaacgcc
    50941 caaaactctg ggatccgaaa acatttcttt tcttatatct tcacttaaag aagcgtcttg
    51001 acctgaagcg cgggaaacta ttaaacctgt ggaagttgat accaaaagag caggaatttg
    51061 ggtaacaaga ccgtcaccta ctgttaagag agtgtatgtt gaaacagcgt ttaaaaaatt
    51121 cattttaagt tgaatcatcc cgattatcaa tccgccgact atatttacaa tagttattac
    51181 aatacctgct gtagcatcgc cttttacaaa ctttgaagca ccatccatgg ttccgtagaa
    51241 attcatttcc cgttctattt ttcttcttct tgcttttgcc tcttcgtcat ttataagccc
    51301 tgaatttaag tctgcatcaa tactcatttg tttgcccggc atagaatcca atgtaaacct
    51361 tgcggaaact tctgcaaccc ttgttgcacc acctgtgatt accataaagt taattattac
    51421 aagaatcacg aatattaccg aacctacgac atagtttcct cctactataa attcgccgaa
    51481 agattgaata acgtttccgg cttcgccata gagcagaata agtcttgttg aactcacgtt
    51541 aagacccaac ctaaaaagag tagcaataag caaaatagta ggaaaagtcg aataatccaa
    51601 aggctctttt gtataaagac aaaccaaaag gattactatt gaaagagaaa tatttatggt
    51661 aagaagaaaa tccaaaagcc ccggaggaat aggcaatacc atcattgcga caatagttac
    51721 aagccctatt gccaaaataa cgtcgttatt cttcattatt actgctaatt ttcccaagtc
    51781 catttttaaa aaacatccta tatatacatt ttactttatt ttttgttttt ttgctcctct
    51841 gttttgccca aaatctaatt tttatctttg ttataaacgt aagccaaaat ctctgcaaca
    51901 gcaacaaaca tatctgccgg tattaccccg tctaaaggta caagtttata taaagcctgc
    51961 gccagaggtt tattttcgac aatcggaata ttattttcct gtgcaatttc tctgattcta
    52021 aatgccacat aatccacccc tttggctata accctcggtg ccggtgattc cattttatca
    52081 tattttaagg caactgcata atgtgtagga tttgtaacaa caacatcagc tttggggata
    52141 tttgcaaaca ttctctgcct tgccatttta acctgaatag aacgtatttt tgctttgatt
    52201 ttagggtctc cttcggtatc cttcatttcg tcttttactt cctgttttgt cattttaatt
    52261 gatttttcat attcataatc ttgatacttt ttatctaaaa gccccaaaac caacattgca
    52321 agacaaatat ttacgaccat ttgtcctaaa attgaaccta taattgcaaa agcaggctca
    52381 atatccatac ccaaaagccc gaatagttca tcttttcttc cgttaataac agaaaatcca
    52441 cacataccta caacagcaag cttaataagt gatttcacaa attctacaat agtgcggggc
    52501 tcaacaggat taaaaagttt ttttgcattt tgcaacattt tcataggtga aatattactc
    52561 aatttgagct tgattttttc ttttgcaaaa acatgcccga tttgaagacg aataacaata
    52621 gtgcccataa ccattaaaaa aagcagaaaa ggcagaataa tttgtcctgc cgttttggca
    52681 aatggcaaaa atacaagtaa aatatcatct gtgctgatat ctttcgggtt aagatgagta
    52741 aatgtataat aaaacaagtt ctttaaatta gacaaaacat aaccggaaaa aatacttaaa
    52801 agggcaactc ccattgtaat cattaaagcc gaaattaaat cctgactttt tgatatatta
    52861 cccttatcgc gctctttacc tcgcctgcgg ccggttgctt tttcggttct ttctgctgcc
    52921 tgattaccca tttatttttc aacctttctt tgtatcagaa aatcaccgca attgcattaa
    52981 acaatttttc gataataacg cctatatgag aatacattgc tgctataaac ataattatca
    53041 ttaccaaacc tatatatatt ttaagcggca ttgctaccat aaaaatattc atctgcggca
    53101 tcatttttgc agtaaatccc aaaagaacat ccgttacaaa caatacagca aaaatcggta
    53161 aagcaatccc aagtgccata gaaaaaattt gccccgacag aaaaactatt tcctgagtaa
    53221 gctgcccgtt aaaaataaac ccgtatccgg gaggaattac cgcaaaactt ttataaacag
    53281 ccgaaaataa ccattgatgc gcatttaatc ctataaaaac cattgacgtt aaaattacat
    53341 atccctgccc taaaatcggt gcgctcattc cggtaagagg gtttaatgcc tgtgctgccg
    53401 caagtcccat ttgaatagaa attatttctg atgccatttc tacccccaca aatacaaggt
    53461 ttgaaataaa cccgattaca taacctatca taaattcctt taaaagtata acgctcaatt
    53521 caggaatggt agaaggtgct tggaatccgc ttttgacaag aactatggga taaattataa
    53581 aagtaattaa ggcgacaaac cagactttta tctgcatggg tatcgggtag gtagaaaata
    53641 tcggcgcaga tgacattaat ccgcccagtc ttgtaaaaac cacaacaaac aaaattaagt
    53701 tatatggaga taataaaact aataaatctg tgggcatttt tacctctaca atagtccggg
    53761 aatggcatca aaaagctcgt gaatatttga tataaataaa ctcatcatcc atggcattaa
    53821 tataataatt gttaatattc cggcaattat tttaggaaca aaagtcaaag ttgattcttg
    53881 aatttgtgtt atagtttgaa aaacgcttac cataagcccc actgtaatac ttacaatcaa
    53941 aacaggtgcg gaaagctcca atattaatat aaaagttttt tgcattatcg atattataac
    54001 ttcttgttcc atttttaatc cttattacgt tgcaaagctt tccacaaggg attttactat
    54061 cagataccaa ccgtcaacca ttacaaacat aatcagctta aaaggcaaag ctatcattgt
    54121 cggcggcaag aacagcatac ccattgatac caaaatactc gacactacaa tatctataac
    54181 caaaaacggc agaaaaatta caaagccgat tttaaatgca gtgtttaatt cacttaaaat
    54241 aaaagacggc aacaaaatat atgtcggaac atcttttttg ctcttgggtt tttcgatttt
    54301 tgccattttt ataaaaagag ccagattctt ttcgtctgtt tgtttaaaca taaaatctcg
    54361 cgcaggaata attgccttat caagagcggt ctgctgggta atttgatttt tcatataagg
    54421 ctgaattgcc gtttcattaa ttttgtttat agttggagac ataataaaaa atgtcaaaat
    54481 caaagccaaa cctgataaaa tctgtgtcgg gggaagcgag cctaccccta ttgcctgtct
    54541 tgtaagcgca agcacaatta ttattcttat aaaagaagtt gccattataa aaatacttgg
    54601 ggcaagtgtt aaaatagtca tccaaataag aatttgaacg ccgttagaaa agtcttgcgg
    54661 agtgtttgca gctttcatcc cgatatttat attgggaaat gccattgcgc aatgtgccat
    54721 tggcacaaat aaaagcattg ccgctaatac aaaaagcatt tttttcaaaa tacccgactt
    54781 catttttcct ccaaacttat actaattttg acaatactcg tcccacctta acataataac
    54841 agctaaggaa aaataagacc aattcttaac taaacttcaa atagtatata ctttttaggc
    54901 ttttttaaac caatttttgt tgaattttgt tccgacatta tattttttgc aataagtttt
    54961 caaatcgtct ttaagttctt ttgccataat atacatcgca agacagtttg gaaccgccat
    55021 acccagcatc atagagtctg taagatttat aacggtatta acattcataa tcgcgcctaa
    55081 caccgtaaac attaaaaatc ccaattgata aaataaagtt ctttttttac cttcgcctac
    55141 tatataattc catgcctttt gtccgtaata tgcccaagaa ataagggtag taagtgcaaa
    55201 taaaattaca accacagaca aaatatattt aaaccatgga aaaattgatt caaacgccga
    55261 tgaagtaagc tcaattccgg taacacctgt cgtatagttt ttataaacac ctgaagaaac
    55321 gataattcct gccgaaagca aacaaacaag ccctgtcaaa aaaggctcga taagtgcgat
    55381 aaaaccttgc gaaatgggtt catctgtctg cgccggagca taaacaatcg gtgcagaacc
    55441 ggtccccgcc tcgtttgttt gaacagaacg cctcattcct ataatcataa tcccgataat
    55501 tccgccggca acagaagaag ggaaaaaggc ttctttcacc attatataaa aagtgtatgg
    55561 aagattatgc cagtttgcga ttaaaattat aatccccgca aaaaggtata aataaatcat
    55621 aaaaggaaca gctttcatcg caactttggt tatagcctta actccgccga taataatcat
    55681 tcccattaaa atcgcacaaa ttaagccgat aagccagtta taaccgtgca aaattcctgt
    55741 ttcgccgcct gcgatattaa taatttgcct tgtggtttga ttaatctgaa tcatattccc
    55801 gccggtcaaa gccccaccga tacaaagcca agcaaaaata acggccaaag gttgaccgag
    55861 ccagcgcatt tttcttcttg taagcccgtg tgcaataaag tgcatcggac cgcctgcgac
    55921 tgtcccgtca ggtagaaatt ttctgtactt taatgaaagt gaaacttcgg cgaatttaag
    55981 cgtcataccc aaaaccgccc caaacattat ccaaagagca gccccggggc ccccgattga
    56041 aattgaaata gcaacccctg ctattgtacc gaggcctacc gtgcctgaaa gtgccgacat
    56101 aagtgctcct aaaggagaga tttcaccctc ggcgttgcct tctttaggct tgcttaaaat
    56161 ttcaaaagaa tgcttgagcc cccaaattcc tatccagcga aagtaaaagg tgaaaaataa
    56221 tcctgcaata agaatccata aaataattac agggatttca accccaaaaa gcgttacggg
    56281 gaaaaacatt atgcccgaaa ctttttctga aacaggggtt acgtatttgt ctatttcttt
    56341 gtccaaatta aacgcaaaaa cactttgcaa actgcaaaaa aatgccgcca atgttaaaaa
    56401 taactttttc atcaataatt cctcgctagt ggtctttaac aatcgtagca aaaacaacat
    56461 tcaaaaaaac tgtttcttta agaatttgtt gcaaaaaaat aattaagaaa tattaatttt
    56521 aaatctaaga aagcaattat ttttatgttt gttttttatt aaaatgttat gagttagtta
    56581 aaaaacggag aaaattatgt taagtattgc gggaatatca agacaagttg cttttgaaaa
    56641 aaaacaagat aagcttaatc ccaaaaaaac cgcccctcgc aaaattgcac cttctgagga
    56701 ctgtaatcat ttatgctata tagctcacga tggtacagaa attcctactg acaaccctaa
    56761 agccctaaaa ataattcaaa aaataaatag tgattttcat aaaaaaatga aagcaaaaag
    56821 acctcgttca ttcaaagaat tagtagcaac aaaaaaaatt aaaatataaa aacatacttt
    56881 atttaatatt ttctaaataa tttagggcac aattttgttc acaaaaagat ttagcagtat
    56941 ttatagagtt ttgtacaatc ttttctttat cttcaaagtt ttttatcttt aaaagaattt
    57001 cttttattcc ttcaattgtg ggctcgcata aaaatccgtt ttcgccgtct tttattatcc
    57061 catcgattcc gtcatctttt gtgcaaacag taatacatcc cgatgccata gcctctaaat
    57121 ataccatgcc aaaagtttct tttacacttg gcagaataaa aatgtgggaa tttttcatct
    57181 gctccaaaac ctctttatta gaaatttttc ccacaaattt tacgtttttt gaggcgattt
    57241 ttcttaattt tgaaagctct ttgccttctc cgataacctt taattcaaag ttttccaaat
    57301 cgtttattgc aagaattaat ttattaacgt ttttgcgttt gattaaattg gcgcaggtaa
    57361 taatagtgag aggtgagagg tgagaggtga gaggttcgta ggttggggtt ttgtctgtcc
    57421 tcctcccccc ttgaggggga gggtagggga ggggggaatt tatcccaaca acggaaatcc
    57481 ccgaaggtgc gacaaaggtt ttgtctgcca agtcgggata cagttcacag aacttctttt
    57541 gtaaaacaaa agagcggcat gcgatttttt tggaattttt ataagcctct tccagttttt
    57601 tcttaaaata aaatctgtac atcagctttg tcaaaatctc aatatctgag caatgaaccc
    57661 cgcaaacaag cgggatatta tatttttttg ctattttatt tgccaaaata attcctgagg
    57721 gcatgtgtgc cacaataata tcataacttt ttaaatcaat ttccgttgga agtttttttg
    57781 taatatcaaa caaaaaaggg ctaaagtaat ttacgttaaa aattcgtaca ttttcatatt
    57841 cataaaaacc gtttttgtga aaaaccttgc cccttaaaaa gctgtttagt aaaaaattcg
    57901 gccttatcac atcgacctga ttttttaatt gtatccaatt tgcaacaaaa cgatgtaaag
    57961 tcaaagggca attcttttcc ccttgcttta caggatataa gtctgttaca aacaaaattt
    58021 tcataaaaca agtataacat attttaaaaa actatatatt tttcaaaaaa agttgctata
    58081 atagacttaa tggcaaatgt tggaaaaaaa ttatctgttg cattctactg gcactttcat
    58141 cagccgccct atcagttaag cgaggaaagt gattatctta tgccctgggt gagattacac
    58201 gcccttaagg attatcttga catgcttttg ccgataaaag attttccaaa attaaaatta
    58261 aattttaact ttgttccgac actgcttgat gccttaattg aatatggcga aaagggttat
    58321 catgatatac attcaagact tacggtgacc cctattgaag aattgtcggc agaagataag
    58381 gaatttattt taaataattt ttttgatgca aattacactt caatgatttg tccaaatgaa
    58441 ccttacgaca ggctttatca aaaacgctgc aagcctgaag gcacaggtat tgaagatttt
    58501 tctaatcaag aatttgccga tataatggcg tggtttaatc ttgcctggat tgaccctgtt
    58561 tttaaggaaa tgtttcccga gcttttggtg cttttagaaa aaggcgaaaa ttactcgctt
    58621 gaagatagaa taaaaataat tgactttcac agaaaaatca taaaaatgat cattccaacc
    58681 tataggcaat acttagaaca aggcaaaata gaaattacaa caagccctta ctatcacccg
    58741 attttaccta ttttgcttga tgtaaaatcg gcacaaaaaa atctttcaac acccgatact
    58801 gatttaactg atttaggcat ggaagaagat gcgaaattgc aaacaaaaat ggcgcttgac
    58861 cgaatcgaag aacttttcgg caaaaggcct aaaggaattt ggccgcccga gcatgccatg
    58921 agtccggatg agttggaaat gctcaaagaa ctcggtgttg aatggacaat ttctgatgag
    58981 gggattttgt caaattcgat tgattttgaa tttgtaagag attttaaggg gtatttggaa
    59041 gatccatact tcttgatgaa atcttacaaa tacaagacaa aaagcagtga tataaaactg
    59101 attttcaggg attctgttat tccgaacctg ataagttttg agtattcaaa tcacgattca
    59161 aaacttgcag caagtgatct ttacgacagg ataaaagtca ttcaaaacaa attgaaatct
    59221 tctccggatg aaaatcattt gctcactatt gctatggacg gcgaaaattg ttgggaaaat
    59281 tatcctcaag acggaatgat ttttttgagg aaaatttatt cgcttttgga aaaagatgat
    59341 accatagaaa ctgttctgat aagtgactat ttggaaaaag aaaaaaacca taaaaaactc
    59401 gataaaattt acacaggttc atggataaac aaaaatttcc agctttgggt ggcagagcca
    59461 gttaaaaata ttgcatggca ctttttgaaa agggcaaaag acgattttga agaattaaaa
    59521 cgaacaaacc ctgacaatcc tgatatagaa gccgcacata aagaacttat gatagcacag
    59581 ggcagcgatt ggttttggtg gtttggcgag cccaacgatt ctggacaaga ccatgttttt
    59641 gattatatgt tcagggaaca tttaaaaaac atttacagat actttaatgc agattatccg
    59701 cagcggttgg atgatccttt tctttccgtt agcattgaac cggtaagata tcccaaatgt
    59761 aatttcactc cagccataga cggaatggat ttccccgaag atcaatggga aaatgcaggg
    59821 tgtattaata tgcctgatgg tccttcaagg caagaaaaca agcttttcaa cagaatttgt
    59881 tacggggcgg acaaaaataa tttctatctg agaatttatt taaatgactt tttccaggaa
    59941 accttccttg acagtatgtt tttatcccaa ctttatgttt atatgaaaaa cggaaacaga
    60001 actcaaccgc tttctccgat gaggcttgtc aatcagaccg aaaacatttt tcctatcatg
    60061 aaagaaaaat ttcataacga aataagactt tccattataa ataaaaaact ttattcaatc
    60121 cgtcttataa aatcaatgcc cggcggagtt tgggttgcaa aacactccaa agatatgaaa
    60181 gttgcctttg aacaggtttt tgacgtgagt gtgccgtttg atgatattga tataaaaagc
    60241 ggagaaaaaa tggaattttt cttcgcaaac gccaacttcg gaataaaaga cacatttgtg
    60301 cctcaagacc acttgcttat tatccaaaga ccataaaaaa ttgaagggtg aagggtgagc
    60361 ggtgaaaggg tgaaaagtga aagattaaat agttgataag tcgaatagtc gaagagttga
    60421 aaagttgttt cgtcattctg agcggcggcg aagaatctca cgtcattgcg agacatgagt
    60481 tattggcttt tacaattttt attcaccgaa gcaatctaaa aaccggtgca caaaacacac
    60541 cttaagcttg ccttcctgca ctcttattca ccatttacta tttaccattt attaatttaa
    60601 aaaactgtga gaataaattt ctttattgag aaggattttt gctgttttaa ttatttttac
    60661 ctgttcaata tcttgagtat caaaagccac atcttcgatt tgcccaaaat ctcttatcat
    60721 ctcagaaagg ttaatgcaaa gctcgtctgt ggagcttttt ggttttgagg tttcaagcat
    60781 ttttactgtg ttaataaggt caaaatcaag cgagttaatt atggcactgt caagtcccgc
    60841 cccgtatgcc agtatcaaaa aaattttgtt tatgagcggt ctgatttcag caggcgagcc
    60901 gtttgaaata tttgaaagcc ctattgtggt cataacctca ggctcaaagc tttgcttaat
    60961 catctgaatt gtgtttaggg cttcctgtgc ctgatcttgg gctacgctta caggcaaaat
    61021 taaaggatca aaaaatattt ttttattttc tattcctttt tcggatgttt tttcgatgat
    61081 ttcaaaagca agattaagtc tgtcatcggc ggattttggg attccgattt gtttgttcat
    61141 ggttaaggcg attaagtttg aaccaaaatc cgccgtcaat gaagtcattt tttctaattt
    61201 ttcttcatcg gcagaagtgc tgttcagcag acaattttgc ggtgatttgc ttttttgaag
    61261 tcctgctttc atctcatcaa agtttgttgt atcaaaagaa attttaaggt ttgaattttg
    61321 ttcgaccaat ttacaaagcc agggcaggat gccttgcatt tgatttttgg caggtcctac
    61381 gtttaaatcg atataatcaa ggtgcgattg ttttttgatt aaatccagaa tgaaaaattc
    61441 atcgcgttca atcagggcat tttgaactga ttttgaaatt atatgaatgt tttcgccgat
    61501 taataacatt tttttattat acataaaaaa agttagaaaa attgtaaatt aatggtaata
    61561 tgttcttgta catatgatta ttatgagagt atgcttatta agtaggaaat taattaagga
    61621 gaagttatga ataaaaagat ttttgctgtt ttgttgggtt tatcaatgag tttgccggtt
    61681 tttgcttcaa agctggaagg acaggtgaac aaacaaacct cgccgattat tattcaagag
    61741 caggagctaa ctatcgggac cgtacaaaaa tatgtacaca acggaatgtc tcaggatgaa
    61801 gttgccatat ctttgggctc gccaaacatt gttactcagg attcaaacgg aaaagaaaca
    61861 tggatttacg acaaagtagc ctcttccata acaagcagag ataaaatgga aagcatgatt
    61921 gaaaacgaca tgggtgcaag atatttttgg catggtgtcc ttacttttgt gacactcgga
    61981 ttaattaata aagacgttaa acctgaaatg aaagatgttg cacagctggt tactacacaa
    62041 aaaactttga ctatagttat taaatttgac agtacaaata aagttgaatc atacacctat
    62101 cacatgagca aattttaatt aggagaaagc atgaaaaaat atttaatttt attattaata
    62161 tctttagtga tatttgtcgc cctgccggta tttgccgcaa aagcacctgt ccagcaggca
    62221 acgcagcttg aaaaaagggc ttatcagaca agatcctttg acgggttaga caaagctact
    62281 attatgaaag caatgctgaa tgtattgcag gatgaaggat ttattgtcaa taatgcaaac
    62341 ccgcttttgg gatttatttc aggctcaaaa gaatttgacg ttacggacaa aaccattgac
    62401 gttcaaaaag aattcggtat aaaaaaagga gcgcttgcca tgaaaggcat aagagttgct
    62461 acgattgaag caacagcgaa tgtttccgag ttcggaaaag aaataagagt gagaataaat
    62521 ttcaaaagaa aattgcttaa tacttacggt aatgcccaat ttattgacga aatttcagac
    62581 gaaaaatatt atcaggattt cttttcaaaa gtagataaag caatatttat tcaaagacaa
    62641 aaaatatagt tgaaagttta ttatgaaaaa aataatttcg gtttttttag ccttaacttt
    62701 ttcattttat tgtgttttgc cggtaagtgc ggcaaaaatt aaagtggcaa aatctcaact
    62761 ggaagtcaga gaaattcaaa cacatatatt tgatacaaac aatataaacg aagttttcaa
    62821 ggctgcgata aatactttgc aagacgaagg ttattcaatt ttaaacattg aagacgagtt
    62881 aggatatata caggcaagaa aagaatataa agcggttcgc atagataaag gcaggatgac
    62941 gggatattat tttttattag cggaatatat tgctttaaca gcattaacat atggaatgga
    63001 agcgcctata atggcagatg cgataagaaa aataagtaac gaacgaagcc cgagaacggt
    63061 agttgttgat tcaaacgtaa caatagagcc ttacggtaaa aaaaccaaag taagatttac
    63121 actgattgaa aaagaactgg aaaacgcaga cgggtattca tatatcaaat catcgccgag
    63181 aaaagtttta agaatatata cagagcctat ttataaggaa ttttttgccg aaatggacaa
    63241 aagcattttt tatggtaacg caaagctgta attttttatg ataaaattat agataaggag
    63301 aggtgtccga gcggcttaag gtgcaaactt ggaaagtttg tgtgggggca acttcaccgg
    63361 gggttcgaat ccccccttct ccgaattaaa gcgggtatgg cataagccat acccgcttta
    63421 attataccct cacagtgcaa gtgagacaga gagaaaacct gagaatttac aaacacaccg
    63481 ccgattgagg cggtttttgt atttggagat gcacttgcac taattaccag ttatctgcac
    63541 tatttgtcct attacttgca ctgaaatgtt gtcatgctga atttatttca gcatctatcc
    63601 cacttcgtgt ttaacttcca gccctatgca atttagtggc aaacacgcac aaaaggctaa
    63661 attcacgggc tcattaagcc ttttggctgg ttgcacccat caaactacgc accgctttgc
    63721 ttgtgcttgt ttgttgtgca attctttgca atttcaaatt atcctgcaaa acaagatgtg
    63781 tcggaatgtc tctgagattg cgacagatga cttgatgtct ttaaaacgag agttatactg
    63841 tgaatgacgg taagaataag gattcacagt atatgaacga actaacagaa acacaaaaaa
    63901 taatcccaga gcatgcaaag aaaattgctc tggcgaaaat ggatttgatg aaacaatggt
    63961 ctgaatttcg caaaaaatcc aacaataaat tcaaagcaga ttacgaattt gttcaactct
    64021 acaacacaag taattcaaat atttttagca tacttggaaa aatttcacga ggaactttac
    64081 accgttggaa agcaaaactc agcgacagcg aagattatac aaaactactt ccgcagtaca
    64141 aatattcaag cgtaa
//
LOCUS       DAOQAU010000004        64119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_311_length_64119_cov_6.880619, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000004 DAOQAU010000000
VERSION     DAOQAU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 64119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64119
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_311_length_64119_cov_6.880619"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKI94_01190"
     CDS             complement(<1..202)
                     /locus_tag="PKI94_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25385.1"
                     /translation="MKFNIFSRLLPPENKVFYDCFEDSAKNCNDIAKLFNEAVINGIT
                     DELLVKAKTLKHRGSGLERETIN"
     gene            complement(342..1019)
                     /locus_tag="PKI94_01195"
     CDS             complement(342..1019)
                     /locus_tag="PKI94_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW25386.1"
                     /translation="MHDNQSKIKEYIDIIQKVAKVEHRRIPSHMVEYEELVSIGIIAV
                     QIMIKNKTPEQLEKYNAAYIGTAVRWAIRNELRNRYKWYSLKHNKTEEDIGETSHGEE
                     SDEEGLDVSPTKVREAIYETILSIDSLAAAASDTDSPFDFVKDTSAMPDEKAEISEMG
                     RMIREAISTLPQKDRTIVEYRFYRNMQVKDIARQVGLSSSRITRIVQQSLNEIREYLQ
                     TKEHFEY"
     gene            complement(1028..2125)
                     /gene="flhB"
                     /locus_tag="PKI94_01200"
     CDS             complement(1028..2125)
                     /gene="flhB"
                     /locus_tag="PKI94_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNW25387.1"
                     /translation="MADEDKQFEATPRKLEQARKEGQVVKSKDFSTAISLLLLFTAIY
                     ALSPFIWDQITKLFVLCYEQIPNKHLSDIGLPYVLTVAIVPTLLIIGPILFLAWFVAI
                     MADFIQVGPLVATAPLVPKLDKLNPSKYFKNLISVKTLFELLKNILKVIILGWIGWSV
                     YSAHITDILRLAAIDNNFAVMMEFGKLIVEFVFKACIAFLAISGADYMVTRWKFMKDQ
                     KMSFKEIKDEYKNTEGDPNVKAALRQRRQQMLQQSMMDSVPTADFVVRNPTHIACALK
                     YDAEEMESPMLVAKGTELIAKKIINIATENGVPVIDNPPVARAIFRVVEINQIIPPEL
                     YKAVAEILLFVYNLKNNQNQGRIMDKPDTSN"
     gene            complement(2263..2913)
                     /gene="deoC"
                     /locus_tag="PKI94_01205"
     CDS             complement(2263..2913)
                     /gene="deoC"
                     /locus_tag="PKI94_01205"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNW25388.1"
                     /translation="MKNLNRYIEHTLLKQDATKKELANLFKEAIKYKFLGICVNPVNV
                     KLAKANLKNSGVKIVAVVGFPLGASKSEAKAYETKLAIKDGADEIDMVLNVSAMKNKD
                     YKTVQEDVATVKKACGKKKLKVILETDLLTKEEIKKACEICIKAKADFVKTSTGFVKN
                     GVGAKVEDVELMYKTISPFGLEVKASAGIRDKEAAIKMIKAGATRLGTSAGVEIVK"
     gene            3066..5897
                     /locus_tag="PKI94_01210"
     CDS             3066..5897
                     /locus_tag="PKI94_01210"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNW25389.1"
                     /translation="MKINPIISFKSNIEALKTPPHNAVATNPIQNSQDLQATALDYKV
                     KTPMKYSDLGELELPCSTKGHLYKLENGQRVVIITKIGPTVVKTYVNSGSMNEPDKFR
                     GISHFIEHNLFNGSEGLSAGQFFDTVNKMGANTNASTGFAATDYYIESNLLKKSDLEK
                     EIKIHASMLEKPTFALDMIEKEKGPVTSEINMILDDPENIATNNTLKMLFNINSTSED
                     IIGGTVDNIKKITREDVIDYYKKNYSPQNMVTVITGEAEPQEAIGLVSKYFSSNNTNP
                     KPRNYEDLKPIQKAVRRDIISDKTPASIISIGFVGPKNIDTKDKILLDAFQFFMIGSN
                     VSRLNKSLERIQTNALMTSDRISTRPQDNTVILFSTQVSEENSEKAIQMIFSEIANLE
                     NDPPSEKELNIVKKKLKLNLAQVFENSQLINTAVGTSMLDNDLKSVMEFEKVIDNMTS
                     SDIVNFAKNYFNLNKAAMTVVHPNTVNAEAIKQNYQRTHNVSFTGNLDEINHKTALDL
                     SKVKRYGITNNFSVATNEIHNNLATFDLSLSAEKPADIKPGVSNILAIMLNRGSKFKN
                     EKDFFSDLENQGVSTQFYASERGIGVTSMFLPQDGKLAMKSAKEVILNPRFKQEELDY
                     AKNILRENIKNTPKISMDGLMKEMFKGQFYGNSLSDILNNLDGIQMADVMSLYNYIVQ
                     NAQGTVAISAPFEKNPDLKSEIFSELCSDFPTLKPSVPSLFNGFIPVRDKSVVVQEHN
                     KSQAEIQMGYKFAVNGNLKDSIVFELMNSILGGTPSSRLFSDLREKQKLAYQVNSRWT
                     NMDNSGILSLYIKTTTDDPQSGQICYDNLQKSLEGFNFHIQKMKNENVTEDELESAKL
                     TLKNKILNASEMTSDKNSSLLSGLNSFYGLSKNNQLLDMIDSISADDIKKCANYVFNS
                     NPTVSVVATKNTIENNKDYLASLGNIVRM"
     gene            5939..6604
                     /gene="sdaAB"
                     /locus_tag="PKI94_01215"
     CDS             5939..6604
                     /gene="sdaAB"
                     /locus_tag="PKI94_01215"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HNW25390.1"
                     /translation="MKNNSLFDVISPVMVGPSSSHTAGAVRLGLLARNIYAQPVKKIT
                     FRLYNSFAQTGKGHGTDKGLMAGLLGFQVDDVRIKKVFELPEAKEIEYDFEYLEDFNR
                     HPNAVDFIFGGKDKMEIFAESVGAGNVRVRRINKFGVDITGEYDTLVLIYKDKPGMLS
                     KVGGIIQGEKINIASLICDRSAKGEDASMCIALDSGLNYSVVQKIKNIEDVYFVRHVS
                     KIL"
     gene            6601..7494
                     /gene="sdaAA"
                     /locus_tag="PKI94_01220"
     CDS             6601..7494
                     /gene="sdaAA"
                     /locus_tag="PKI94_01220"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNW25391.1"
                     /translation="MRKEITTFAELLYACGQKKKRIYELVVQREAFLLDLSEDDVRKK
                     VKVSLDAMKEAIKHGLKSDELSISAKCGADCKKVLEKFSKKPSLFGNLFEKITAYALA
                     TVEENLRMGKIVACPTAGSCGIVPSVLVAVSEELKLTEEEQINALIIAGEIGSIVANK
                     VALAGAVAGCQAECGVAAAMSAGALAYLLGASERQIVHAVALALKNVLGLTCDPVAGL
                     VEIPCVKRNVFLAIHSVVAVELALAGVESKIPADEVVDALLQTGQLMSPMLKESSQAG
                     LAKTKTAKKIEQKLNKKYQNL"
     gene            complement(7535..9349)
                     /gene="mutL"
                     /locus_tag="PKI94_01225"
     CDS             complement(7535..9349)
                     /gene="mutL"
                     /locus_tag="PKI94_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNW25392.1"
                     /translation="MKRIKQLSKHLINQIAAGEVIERPCSVVKELVENSIDASATKIS
                     VEVSNECRDLRIADNGSGIHPDDILLAFSKHATSKIETDEDLFAISTLGFRGEALSSI
                     ISIAKLTCTTRTKDFEVGTKVECENSQVKKVQTGCAVGTIMEVKNLFYNLPARLKFLK
                     NPKTEFSYIQELIQALAIINPQISFELKNNGKTILKTSGLGDLKEVIKEVYSQEVIDN
                     LKEVIKTDNLSGLKISGVVSTPDFTRSSKKNYHTYINSRMIKCPVFLKSIDMAYKNLI
                     GIGKYPFVVLNLEVNPSEIDVNVHPTKKEVRYKNANQVFAFIQSSINMALSSGGDFGQ
                     RNEIKAASPENVAFAFPKKEESDDVYVKEEAFEQAILPVYPSPNLQTFEDNSSHVANF
                     PSPARGEEDNFSPFTLYSSPCSSLLTAHCSPTIIGQYKNTYILIEKEEGLEIVDQHIA
                     EERFIYEKLKKQKQAASQLLFISDVLEVSASEAQLIKENKEKFEKFGYGIEFLKDNQL
                     TFKKVPQLLAKANPKDLLADILENLQGDLDGLEEKILISMSCKASIKAGQKLNMWQMQ
                     EIIQNWQTCEKPYTCPHGRPISKTIPHKQIAAFFERAE"
     gene            9464..10357
                     /locus_tag="PKI94_01230"
     CDS             9464..10357
                     /locus_tag="PKI94_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNW25393.1"
                     /translation="MARYSTKSNLAPKKVEKHLIPDLKVVDKNAIKTTAYSDINLKKW
                     RDYSHIKTDTLWEFPSRLKEGGHSNEYHGNYIPQIAQQLYERFTKKGDVILDLFLGSG
                     TSGIEAVNMKRRCIGVELKEELAQKVSEKFSEKQLVTDVNIICADSTSKEAKEKIQAR
                     LDIMGKKQAQFLVLHPPYDDIIKFSDKKEDLSNCSSTEEFYNLFEKVAKNGYDLLEKG
                     RFAVLIIGDSYKNSQIQPLGFECMRRMNNLGFITKSIIVKDIQGNEKAKGKTANLWRY
                     RALAGGFYIFKHEYVMIMQKV"
     gene            complement(10354..11721)
                     /gene="mgtE"
                     /locus_tag="PKI94_01235"
     CDS             complement(10354..11721)
                     /gene="mgtE"
                     /locus_tag="PKI94_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNW25394.1"
                     /translation="MLNVENITERIREIIDDENLEQLREELEGYHSADLADFFPELKP
                     EERLLCFKTLDEEKAADLLEYLSPQLQVEILGDIDEELASRVISKMPHDSAADVLGDM
                     EEDESETYLDKLPRKFSSEVRELLTYNEDTAGGIMTPVVMTVNVDMTVQDVLSHIREK
                     AQEDNIELYYIYVVDKQNHLLGVISLRKLLTSPIGQKVEEIMITDIVKLHVDDYQDYI
                     ADIFMKYQYNALPVVDLYNRLKGMITWDDAQDIVEEETTEEIYTSSGISTDVVDEDEI
                     LSGNLLKAIRARTPWLFITLIGEFIAVQVGNHYESTLHAVPIIAIFMPLLAGLGGNIG
                     TQSITLMVRGLSTGQVTLSSAFKHIFRETFIGFLIGSVFGLLVMLVTWGWRDNIYLGL
                     VVGLAMLINMTMATVIGTFTPFALKNMKIDPAVASGPMIATAIDVVGLIVYFTLVSIY
                     LAKIV"
     gene            11815..13635
                     /gene="argS"
                     /locus_tag="PKI94_01240"
     CDS             11815..13635
                     /gene="argS"
                     /locus_tag="PKI94_01240"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNW25395.1"
                     /translation="MLKDFFLEEVENAVEKAVKENKLGEMKSYEKGTLSVEKPKNSEF
                     GDFAINVSALARFARLLPAQIAQEIVSALKPQNGTVTVVGGFINFKAGEELLKKTIEE
                     LFKQKENYGKPKKINKEKILLEYVSANPTGPFHIGHGRWAAMGSALANLLKFYGHDVT
                     QEFYINDAGSQIEKLGKSLEIRIKQELGEKIDFPQDETERKNYYTGDYLIPIAKKYLT
                     ENTPHPSPYLSQNDTQSFCSAESQGERENYVSSPFTLHPSLLSDYAKKEMLALQKKLL
                     KEFRVDFDIFYSELDLHNSGKVDETVEKLNKMGKLYEKDGALWFKSSDYGDDQDRVIK
                     KADGSNTYLTADIAYHLDKLERGFDRMINIWGADHHGYVARVKASIEALGYNPESLEV
                     LLGQLVNLVINGEEVRMGKRKNMVTLDELIEEVGVDATRFWMCMRNIDNTLDFDIELA
                     KTSSDENPVFYVQYAHARACSILRNAILEKIDTQTGEKTSPPISQSELDSLIKNFDKT
                     ILNPIFEEKETLESAKRLILKLEEYKFLIKTAAENRAVYALCRYAQELAGDFHSFYTV
                     SRVISEDKALMKARLSLVIAVKTVLANVLKLLAVSAPESM"
     gene            complement(13639..14622)
                     /locus_tag="PKI94_01245"
     CDS             complement(13639..14622)
                     /locus_tag="PKI94_01245"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNW25396.1"
                     /translation="MDNYTPAKLIEYIRDGLIEQEHLGFVVKVGKNIQKIGNDKNYPF
                     YLRSCAKPLQASLLIDYEIDKTFNMTDEEIAVCCASHAGELCHTQTIEGLLNKINLSK
                     ENLKCSTHVPFSRSAQKKLLIEGLKESVLQNNCSGKHAMMLSICKKMGWEVNDYYNID
                     HPLQIAIKKKIYDLCEVKKEYTSTKDGCGVPIFAMPLENMIKGYLNLFFDKKYEKIKN
                     AFLNKPYFIGGEERIDTAIMCANPDLIAKIGAGGLCIVINLKKKEGIMVKIMDCDMKA
                     RSIVLIEALKQLKWLDKDMLENPLIKFQNQRNILTLHLEKVGEITPVFNLT"
     gene            complement(14615..15364)
                     /locus_tag="PKI94_01250"
     CDS             complement(14615..15364)
                     /locus_tag="PKI94_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25397.1"
                     /translation="MKKNMFMLFMDKLLSFPLWVKQIIYLYLEKDLASSLSTDFIKRS
                     EKEIFHLHIPILSFIGKTELNERKSGLDDNIYNFLSNANEGLSILEISMNNFWTMEEV
                     AKYYIFCLEQNYIKTPESPYIHAMAGFMAGKFRTGEYFKRAERISMEQLERAINTQKE
                     SLNTEKPMKMAEVLISLGYVTEKDTASLLLIKEEASKRFILDTSIVPDNFLRDLTQTK
                     AHKEEIEKLTEQNRVLKEQLNRILAFVKKNG"
     gene            15461..15913
                     /locus_tag="PKI94_01255"
     CDS             15461..15913
                     /locus_tag="PKI94_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25398.1"
                     /translation="MGLDGFSMSNLGLPERLTSAQMAERAEQLALEGSEYKVKDITQL
                     ASQNGVQRKENESSGFTPGYSGSFRKKKDGEETPEEKEKNQEVLNQVNFEEKDSKDFS
                     VRINPETENIELYSLRNQKVIETIGADDLRDLILKLDDASGVFVNKKI"
     gene            15928..16422
                     /gene="fliS"
                     /locus_tag="PKI94_01260"
     CDS             15928..16422
                     /gene="fliS"
                     /locus_tag="PKI94_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNW25399.1"
                     /translation="MNNLVNPYLKQYQKNQVETASPEQVLILLYDGAIQYLNKAKIAI
                     GMNDIEQIQKNLTGCERIILEFMSTLDIKNGGSLAQNLYKLYDYLYNTLVEINVSKDE
                     KKLEEVLRHLTGLRETWNKAIAMANAERQSNLNDFKRVDIKDDQDYEEGEYFDQDDEE
                     QEDE"
     gene            16434..16802
                     /locus_tag="PKI94_01265"
     CDS             16434..16802
                     /locus_tag="PKI94_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25400.1"
                     /translation="MTQFSQLKLLYNQFFRLSDEIKSMIVNDEFNEAISVVQRKDALI
                     KAIIEAKKTLEIKEEEKSEMEALEKDLKEKELANINLLKDLQHEVAQQLGKTNKSLKL
                     NSAYSAQNDENSGSMLDFSE"
     gene            16816..17697
                     /locus_tag="PKI94_01270"
     CDS             16816..17697
                     /locus_tag="PKI94_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNW25401.1"
                     /translation="MNEEFVKEKKKAAKLSIVSNSFLIVLKFVAGFISGSIGIISEAI
                     HSGTDLLASFIAYFSISKSSQPADKEHPYGHDKYEDFAGFVEGLLIIFAAFYIIHEAV
                     KKILNPELIELDVDLGLLIMGISIIANFFVSGYLFKVAQKTTSMALHADGEHLRTDVY
                     SSLAVFLGLLFVKVTDNPVFDPVVAICVAVIIFFAGYRICRDCTSNLLDTSLSENENS
                     YIRGIIGDFINESEVIEIKNMRTRKAGMKKNIDITLSVNGIMPISQAHELCDRIEKQI
                     EDSIQNTDITIHVEPHK"
     gene            17814..18278
                     /locus_tag="PKI94_01275"
     CDS             17814..18278
                     /locus_tag="PKI94_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNW25402.1"
                     /translation="MKHSLAIKKPDFDFDSFNQEFNNFLRDTLFETSFASPLHLKMTS
                     TWRPAIEVQQTEKEYKVKVQLPGVNKEDIHVDLNNDFMTVTAEIKEEKDKKEASKMHT
                     CEIRYGKYVRTIAFDTPIKTADSSAEYKNGILTVTMPKQKITKTQTKRLTVK"
     gene            complement(18339..19382)
                     /locus_tag="PKI94_01280"
     CDS             complement(18339..19382)
                     /locus_tag="PKI94_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNW25403.1"
                     /translation="MKMKSIQYWGPGDIRFEEISIKPLEEGEVLVKVESALTCGTDVK
                     TFKRGHPVLIKQIPSGFGHEFAGTIAQLGKNVEGFEIGDRVVAANSAPCGKCFFCKKE
                     EYNLCENLDLLNGAYAEYITVPARIVQKNMLKLPDDLSFDKAAFCEPLANVVHGVERT
                     EIKPGQTVGIIGIGPIGLMFAKLAKLKGAKVIAAGRNPLKLELAKSFSQADEVVDLTK
                     YPNPEKIFMEFTEEGKGLDVAVECVGLPSIWERIFSMIRPGGTVHFFGGCKSGSTVTF
                     DTTKMHYGDIKMISVFHHTPKYFKKALDLISSGQVPVEKLITETISLKEVQSALEKHI
                     EGNAIKFLVKPWS"
     gene            complement(19388..20371)
                     /locus_tag="PKI94_01285"
     CDS             complement(19388..20371)
                     /locus_tag="PKI94_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNW25404.1"
                     /translation="MKSAQIKKNRIVIKDAPKPNLKKFGFGAIIKTSGCGLCGSDIVK
                     FRQNFIKEGTVLGHEIVGEIVEINSKTAFKIGDKIVSSHHIPCFKCNYCKNKNYSMCK
                     HFKETNIIPGGFSEYIYLSEEHLQNVAVKILKKMTEIEASFYEPLGCCIRAIKRGGGK
                     YKGKKVLVVGLGSIGLLMGQALKAYGANVFGCDIIQERIDFAKKFKIKAVHSSKTQKL
                     DSDIVFMTAGADATIDLALKAVRDGGKIVVFASTPKNFGYANNEIYYRELTVLGSYSP
                     SPKDLKEAMKLLSSAKVKVKNISTVYDFCDIEKAFRDTIENKVMKAYIKVG"
     gene            complement(20352..20531)
                     /locus_tag="PKI94_01290"
     CDS             complement(20352..20531)
                     /locus_tag="PKI94_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25405.1"
                     /translation="MKKGQTVIEYFLIFVLVALFATVFASKFDFKKIKNYVFSRPSSG
                     PVIEIEPMTNEISTN"
     gene            20619..21623
                     /locus_tag="PKI94_01295"
     CDS             20619..21623
                     /locus_tag="PKI94_01295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW25406.1"
                     /translation="MARIRRLRYFDKFKLKKMISFLSSDVISHYTSVFMKFPFNTLHE
                     FLPLRFKFMPETHVLTQNGEILGMITVSPTPGNPFRLAINRLFLEQNYYNAGKQLIEF
                     VVAKYGAKGASAFVAAIDESHGELLQLFIEGCGFRHCSSEQLWKMSEFHFTKENHAFI
                     RPFKNSDAQSVAMLFNDSVITHFKHSIEKKKNEYQDPLFQGLRNIYKLKYVIEDEHTK
                     TVKAYFSLSTTDNMNYILDITTSPWYDCSYEDIFAFTVNQITKRKKDFSLFVKLKKYT
                     VKAEIFEEFLRQKGFNCVQTHLILVKDFYKLVKEPESALKIVMFNEASEKPVFKIEGF
                     "
     gene            21638..22726
                     /gene="ribD"
                     /locus_tag="PKI94_01300"
     CDS             21638..22726
                     /gene="ribD"
                     /locus_tag="PKI94_01300"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNW25407.1"
                     /translation="MKKFEKYMKKCLKLAQKGDGKTSPNPLVGCVVLDKNGKEISSGY
                     HKKYGSLHAEADALSKLKDNQIKGSTLIVNLEPCVHFGKTPPCADLIIEKGVKTLVVA
                     MKDPNPVVSGKGIEKCKKAGINVLEGILEEEARQLNEVFIKNMEKRAIFVATKIASTL
                     DGKIAAQNGKSKWITGEYAREEVQKIRNRYDAILTTSATVIKDNPSLTCRIKNGKNPI
                     RIVLDKDLKTDFKSKIYENNCEKIYVAVDKNTDKTRLSTVPAHVEIIKCPLKASKIDL
                     NFLFKKLFEKEIRSILIEAGGNLNGELIFQDLADKIYLFMAPKIMNDKNAINSFYGEK
                     TFKIDNLARFKISKIDKIAEDVLFILNK"
     gene            complement(22798..23055)
                     /locus_tag="PKI94_01305"
     CDS             complement(22798..23055)
                     /locus_tag="PKI94_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25408.1"
                     /translation="MITVSPIKLNSYKNISFLSNKNEINKDIKSSTYELTDVQFKSVI
                     PKKNIFSKIINKISKFVKDFVKTPPSYEDFDIQDEIFRVLA"
     gene            complement(23611..25107)
                     /gene="lysS"
                     /locus_tag="PKI94_01310"
     CDS             complement(23611..25107)
                     /gene="lysS"
                     /locus_tag="PKI94_01310"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNW25409.1"
                     /translation="MTEEIQEHSSQKDSIRQTRVQKLADLADKGINPYPYSYSKDANA
                     NDLQEKYKDLPVGEETQDFYSVAGRVMAMRNTGMFIDLMDATGKIQIFCHKENMDEEA
                     IKTLKLIDIGDIVGFYGSMRRTPRGELSIKAKNYKLLSKALLPLPEKFHGLTDIETRY
                     RQRYVDLIVNEESREVLRKRSLIIQKIREYLTQQGFLEVETPMLHPQAGGANARPFIT
                     HHNALDMEMYLRIAPELYLKRLLVGGLSEKIFEINRSFRNEGCDTRHNPEFTMIELYQ
                     AYVDYNEMMALTENMVSSVAQDLLGTMKINFDGKEIDLTPPWDRKTMLGAIKEYTGID
                     FNTMYTAQEAAQKAKDMGIEVEETDNWGQILDKIFEEKVEPHLIQPIHIIDYPRDISP
                     LAKVHRDNDRLTERFESRINGWEIANAFSELTDPIDQRSRFEAQARAKEMGDEEACDI
                     DEDFINALEYGMPPAGGMGMGIDRLTMLLTDSQSIRQVIAFPTMKKIG"
     gene            complement(25350..27443)
                     /locus_tag="PKI94_01315"
     CDS             complement(25350..27443)
                     /locus_tag="PKI94_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNW25410.1"
                     /translation="MSEFQVPKRIKKAKSTNINPDIANVVLIVVSALLAGIVAFHVYL
                     LSLPPIRNLDEFKPNIVTKFYSSDDEIIKTFTAFTFSKVDLKDIPDNLKNAIIATEDK
                     NFYKHRGYDITGLVRSTFANVLAGHVVQGASTITQQLARILFLSNEKTFTRKIKELII
                     AARIEKTIPKSKILEMYLNNVYLGSGAYGVEGASQIYFNKHLKDLTLAESALLAGLPQ
                     APSVYSPFNNEDLAIERRNQVLKRMYKMRFITKKEYQKAVKEKLKLNPLPEIYTLNKA
                     PYFCDYVMKELEKLGFDEVEISQGGYKVITTIDYKTQQQTNEAIIRNLNGWGLRGEKQ
                     QAAVFSFSPIDGRILAYAGGKDYSKSQYDRVTQAVRPPGSAFKPFIYAAAVEKGLDPN
                     DLIEDAPFKAGDWAPRNYHNKYRGKIPAYKALMISSNVCAARVIQSTGIRSVIQMARL
                     LGISTPLEYDYTIALGSNGVKLFEITRAYGAFANGGYRVEPYGIERVESSRGQVIYQA
                     PKAKISKVIDTNTAAIMTAMLKTVMKSGTGVAANIGKPAAGKTGTTDDYKDACFIGYT
                     PDVVTGVWVGNDDNTKIGNLTGGTVPALIWRDVMKVATAPYGNADFDYPKVELKNYSA
                     AQKEEKAEGEEEITDENATDSTTLENNEILPSDVEQPVKKPSAPATPKQISAPKASKP
                     NQLAPIPMATPAGIN"
     gene            27514..27906
                     /locus_tag="PKI94_01320"
     CDS             27514..27906
                     /locus_tag="PKI94_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNW25411.1"
                     /translation="MFNLFFNIINEIKSYFVVEKTEYEIIGECKKCGKCCNYMYSFDT
                     YTEKEFKFMQFLYPAYKRFYIKGKDEEGNFIFACKYVTKEGLCSVYDKRLKMCKIYPF
                     RKIKYPAKLHEGCGYKVLKKDFEDYLNK"
     gene            27970..29118
                     /locus_tag="PKI94_01325"
     CDS             27970..29118
                     /locus_tag="PKI94_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNW25412.1"
                     /translation="MKKILFKLALGLFALSYMILPSCAKVFPDVADNHWASSQIKELS
                     QAGVIVGYPDGTFKPDANVTRAEFACMAIRALKQENTKVIQPVDFTDINEDYWAYDMI
                     QRALYFELISCPPSGKLFRPDDSVSHAEALTMSVNALTTETISAQKASEVLKVYTDLN
                     QIPDWFLITAGKAEILSMNVIIPNVPRTMNADKPATRAEITVLLSNMMAQAKLNPNKK
                     LAEAMRKKTGDGFVIESAFVQGTVGTIPAGSIIPIQLTAPISSQTSKLGEIYLAKAPE
                     NYITRDKYILIYKNSDLKGKLIDVREGKLFVRNGMLILDNDTLVTPNDQTINFHGVGE
                     VCVKKNWFVAFLRKVFKGEKLKVAPKGTVYVKLLQPVKVDLCNGWILE"
     gene            29214..30524
                     /locus_tag="PKI94_01330"
     CDS             29214..30524
                     /locus_tag="PKI94_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25413.1"
                     /translation="MKKILLSLVFIFVFCPRVFSGDYCAVGVDHFKKGNYEKAMYNLE
                     KAVKASPQNVNARYYLAQCYILQKRNSDALNQYNRIILLAPSSDAARLSVVGISLIEQ
                     SYNGKSVVGVGLLSDELAKYKDNYFDYVVTGDNALFRWASFPVRVYIQPSAQQGIVKK
                     AFDEWQNKTGNLVKFQFVNSAQNAQINVDFLQQLESTSTKEAYVAGYSKPYYKDGKIV
                     KSDVHILATEPDTGQPVNNDFLYFSALHEIGHALGFRGHSPNENDVMFAQAKTPKLSL
                     SQRDINTMNVFYRINEKTLTAKGGGQSDVKLQQAVDYSKQFPEKSVSWTGLGDIYMGK
                     KMYPDAIKNYKKAVAIEPQKADLYALLGNAYSASGDSRNAFVNLRQACDLDKNNGDYV
                     YNFAVFCAKNGQKQIGEQYVKSYLTANPQALSDSKMQSLLQSYK"
     gene            30729..31946
                     /locus_tag="PKI94_01335"
     CDS             30729..31946
                     /locus_tag="PKI94_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25414.1"
                     /translation="MRVRNDFSYDVQKRQGKRDIGFSKFSFGADAPAPAQPAQKVEEP
                     KEKGSFIKKATNFLGVAAWLFMVGYSIKKLRLLEPSYHENVAKSAKNYSKRTLSYIEK
                     LADPEAANISKVLEEKGFKGPSKFVRKAFYGIGDWFQNWKMNMGSELVNNLTYAFGTL
                     VVMPLVVLFSPFGKKNSSKEDKAFAVLRQPLSVAATLGMQFTFDKLIDKYVPEVLKQN
                     KFEEPAIVGENGKIVWKNQNNEFLADNFNKIIYNKDEAKEAFKEYAKNVLSEDEIKEL
                     FTDRSFEEGSTSELKTRLKDILNKKYAEAGLKLDKIENVEVNDINRKASSFSEFKKIE
                     KIAAENIEDLLVKFKKFASVLDNNKMAIQKCKTGVNVVAASIIGCTFLNVIYGKFMKA
                     LKKPQEQQAKEVK"
     gene            31946..32824
                     /locus_tag="PKI94_01340"
     CDS             31946..32824
                     /locus_tag="PKI94_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25415.1"
                     /translation="MTIDISNALNNLNISKIEVKLDETFDYVLKQKEAKKQFKILSKI
                     ASNKDIFDKENIDALNIVKTHLETLKANFETIESVTKKVAGEIKIPKVFNHVVKLLGK
                     VKIDKTGNKALDTASALTHVVLWGNVGKEVVGTAMYTVQALTNEDLPKDKRKFVGMYD
                     LAVGVVSTCFSFIFGVGLENTIKNGYKKLLKPLAESENLALKSRSGAAIVGLAAFSSF
                     ILQTIVGKRMVAPAVATPVAGKLKKKLEERENGKKPVDLTATDLNIGFPKVGDNSQGA
                     GTTNLLDKARALSVKA"
     gene            33080..36610
                     /gene="nifJ"
                     /locus_tag="PKI94_01345"
     CDS             33080..36610
                     /gene="nifJ"
                     /locus_tag="PKI94_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNW25416.1"
                     /translation="MSGKTITVDGNYAAAHVAYALSEVSAIYPITPSSTMGEWVDEWA
                     SQHKKNIWGTEVKVAEMQSEAGAAGAVHGSLAAGCLTSTYTASQGLLLMIPVMHKAAG
                     ELLPHVMHVTARSLAAQSLSIFGDHSDVMGCRNTGYAMLAANSVQEEMDLALVAHLAT
                     YKSKVPFLQFFDGFRTSHEVHKIEEISYEDIAKLVEPQYIEEFKNRALKPEAPICKVG
                     AQNPDVYFQGRETVNKFYDAVPDIVQEYMDKVAKVTGRQYHLFDYVGASDATDVIVAI
                     GSACETIEETIEYLNSKRGKKYGLIKVRLYRPFDVKRFNEALPKSTKRVIALDRTKEP
                     GAIGEPLYMDVVAAIGCQKDIRVIGGRYGLSSKEFTPSMALAVYKHSENNGFHGFTVG
                     IEDDITNLSLKIDEQIVTEPEGTTNCMFWGLGSDGTVGANKSSIKIIGDYTNKDAQAY
                     FAYDSKKSYGVTVSHLRFGDKQIKSTYLITAPDFVSCSAHSYIGRYDLLKGISEGGTF
                     LLNSPYSKDEVFCHLTKDMQETIINKKIKFYNVNAEELIKQQPGLRGKGANTVMMVAF
                     FKVSGIIPFEQAYEGMKNMTVKTFKKKGDDVVNMNLALIDAAINACEEIPVPSSLDVN
                     CVSAAKLLPDDASDFAKKIIEPSMKQKGDDIPVSAMSVDGVIPTSTSKLEKRCIAPRV
                     PHWNSETCIQCGICASACPHAVIRTKLIMPEDLKNAPATFNTIDAKPGDGEKFKVQVY
                     CEDCTGCGVCVDQCPININKPETPALSWSTIEAEKEAGEKENEIFFEALPDNVMGKNT
                     TKTIKGAMLRKPLFEFSGACAGCGETPYVKLVSQLFGENMIVANATGCSSIYSGTFPT
                     IPYTTSKEGRGPAWANSLFEDNAEFGFGMRLAVDQNRETLKLYAEKLLDCEKTPVELK
                     ESIKASLALWDDSKTDEAIKAQDKVKELLAKTDCDCVELAKVKELQDYFTDKSVWIIG
                     GDGWANDIGYGGIDHVLAQGKDVNILVLDTEVYSNTGGQASKATPTAAVAKFATGGKP
                     TYKKNMGLMSMSYGHVYVASVALGADRGQTLKALQEAEAYNGPSIIFAYAPCIAHGID
                     MSKTQTEEKRAVEAGYFPLYRYNPANEQPFSWDAKETKGSYQDFIRSEGRYKQLMKTN
                     PEAAEALYKQAEDDASKRMAVYKAVGELMK"
     gene            36711..37607
                     /locus_tag="PKI94_01350"
     CDS             36711..37607
                     /locus_tag="PKI94_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNW25417.1"
                     /translation="MFNKEKPVEVIIDKEKCTKCGICIGYCHEFLGKDQDGYPKAMPV
                     ENTLAGCIQCGHCMMACPNSAIEIKGEDISKSHLREFGNAANFEELNNLFLKRRSIRK
                     FKKDEVSKEVLDKIIQAAATAAVSIPPSELKVLVISGSKKVQELAEEIVQGMKKFPKM
                     FNPISLNLMKIFAGETNYRMFKEFIIPLCKITVEARDRGEDYLFYNAPAVILFYSTEL
                     SDKEDSLIAATQATLAAEALGLGTCWIGSLGAVFQHNEKLRKKYGILKNEKIGSGFVV
                     GYPDTKFYRAFQRNFKNVTFIS"
     gene            37678..37851
                     /locus_tag="PKI94_01355"
     CDS             37678..37851
                     /locus_tag="PKI94_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25418.1"
                     /translation="MSVNLPIIEKVAGKVFVCSKGVDPVKIECASETIATQIAHQVKM
                     LAITLGKKLDKTV"
     gene            37875..38096
                     /locus_tag="PKI94_01360"
     CDS             37875..38096
                     /locus_tag="PKI94_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25419.1"
                     /translation="MSESIASIVKKDGQIFVNVKPAGAKEGKLGEGGVLVKCKDEEEA
                     KAMVKEIDAAAIKEATLKEGEGKKIDKAA"
     gene            38269..39504
                     /gene="glyA"
                     /locus_tag="PKI94_01365"
     CDS             38269..39504
                     /gene="glyA"
                     /locus_tag="PKI94_01365"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNW25420.1"
                     /translation="MEIEIIKQTDPEVAQFVEAELKRQQATIELIASENFTSKAVMAA
                     CGTVLTNKYAEGKPHKRFYNGCENVDAIEELAQERAKKLFNVAHANVQPHSGAQANMA
                     VFLYALKAGDKVMGMDLSNGGHLTHGSPVNFSGLYFDIVSYGVDENGCIDYNEVEKLA
                     QKEKPKMIICGASNYSKIIDFKRFREIADGIGAYLMADIAHIAGLVAGGVHPSPSVYA
                     HFITTTTHKTLRGPRGGIIMCGEEHAAGIDKAVFPGIQGGPLEHIIAGKAIALLEASK
                     PEFKLYAQQIIKNAQALANGLIGETMDIVGGCTENHLMTLDLRKLNKTGKDMANVLER
                     VGITANKNTVPNDPQSPFVTSGIRLGTPAVTTRGFREDDMDEVAKIIASAVFSSDNEV
                     GLNNLRERSLKLCQKYPLY"
     gene            39517..40557
                     /locus_tag="PKI94_01370"
     CDS             39517..40557
                     /locus_tag="PKI94_01370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNW25421.1"
                     /translation="MIIKLSEAHIIGAIISYLLGVFIVPLVISYSEKQGLIDEPNERK
                     IHRKPISRLGGIAIWLSTMLTFLFLVFLSYYPYGSLVSGILLGSSLMFFLGLIDDIYN
                     LDAKFKLFIQIAIVTLVYFLGVKIDTIINPFGGVIHLGHFSYIITVLWIVGISNAVNF
                     IDGVDGLAGSVITVNSITLAIIAVAMVPPSPISALIGFILAGSMLAFLTYNFNPAKIF
                     MGDSGALFAGFLLAALSITGVMKAAALAIILPFVVLAVPIADITYSSLRRISKGKSPF
                     VADAEHIHHKLLKAGFTQKKTVLILTSVAVFAGAVAAFFAGSIKHYIAYILALIVIMV
                     VLNLIKIFKKSA"
     gene            complement(40610..41422)
                     /locus_tag="PKI94_01375"
     CDS             complement(40610..41422)
                     /locus_tag="PKI94_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNW25422.1"
                     /translation="MDKNLKIGVVGLGLIGGSIFKELCALGFDVIGVSKSAGTIEKAK
                     KYSFATDSLQSLNDCDIVFVCTPMNKTISTLDELENILNENTIVTDVCSLKRFLQSKN
                     RPQKYKFKFIPGHPMAGSEFSGFDNSIEGLFKEANWVLTPLDKNLKEDIKKLENIIKS
                     LGANPVFTTPEAHDEAVALISHMPMLISQAIFKAACCNELALKIASSGFRDMTRLAIS
                     NEEMAKDMINLNSDNIQNALLKLYSSVGDLLNKDYFEQICEIKKQRKEMFKN"
     gene            complement(41432..42772)
                     /locus_tag="PKI94_01380"
     CDS             complement(41432..42772)
                     /locus_tag="PKI94_01380"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNW25423.1"
                     /translation="MQKEVRKSQLGLKGEVVAPADKSISHRAAIILSLVKGKSVVKNF
                     SKGADCLSTLSVLKNLGIETKFGSDGELFINSENGFKAPEKELFCGNSGTTIRLMSGV
                     LAGQNFDCVLTGDESLSKRPMKRIIEPLELMGAKIIASKSPQRHPELVSGSIQESNFT
                     KVCFTAPLKILASKLNGIDYHSPISSAQVKSCVLLAGLFAQGKTTFTEPFISRNHTER
                     MLEYLGAKISVQGNTITIEKSQFEPKTITVPGDISSAAFFIVAALIVKNSDILIKNVG
                     LNPTRTGILEVLQEMGADIEILDKKLISNEQVGDLRVRFSELKACEISGELIPKLIDE
                     LPIIALLATQAQGETVIKDAQDLRNKESDRISAVVSELKKLGSEIEETADGFRIKGKS
                     KLKGGVEVESYHDHRLAMMLYVAGLICYEPILINDFEWVNISFPDFEKLLEDLI"
     gene            complement(42780..43562)
                     /gene="murI"
                     /locus_tag="PKI94_01385"
     CDS             complement(42780..43562)
                     /gene="murI"
                     /locus_tag="PKI94_01385"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNW25424.1"
                     /translation="MNKKPIAFFDSGVGGITVFKKVKELLPYENYIYFGDLKNLPYGE
                     KTSEELYKFVDNILKFFEKQDVKAVVMACNTTSATVYEKLKDNYGFKIYPIIQASAKE
                     ISQIQTKKVGVFATNATIKSGIYAKELKKYNPDLEVFEIATPEWVNIVENQTQNLQEN
                     INLIEKYVKEMLKNNPDKIILGCTHYPYLIDILAQFAPKEMFIDPSVYFADAIKQGIY
                     ETGINDSKIEGFENFFVSANPEDFKKAASVFYSLKDLPKLIV"
     gene            complement(43574..45460)
                     /locus_tag="PKI94_01390"
     CDS             complement(43574..45460)
                     /locus_tag="PKI94_01390"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNW25425.1"
                     /translation="MIKKILLLFLFIAVFIQSALCDEVFKITSVNFDTSNSIILLTSP
                     DNTVEPILKNIKLVKLHEPERAYFDIDSAVLTVPGQNWFFNTGLLKQVKVGQFSTNPN
                     KVRVVLYFDEGASAQKVSFLKVNNNIVIKLKDGICKEDYFQNIYRDERTSSSDFYEYL
                     SISDEDANTVKVAVDTPKQDEVLAQIQKAFNASIAPQVSLTPVKSVVSEGVKKELKLK
                     SKYYLDAIYPKQDGILLSGFGAIGIEKPLYLTEPSRVVFDIPNAVANPKIRNKEIKLN
                     DKETLKIGQFLANKVRIVITTEEKEKYFPVFSSDGQSILISDTKNVDLTSLFSKTTDI
                     VTYYSKQVNPTTSEYIFAFNTAVVTGIQRDDSKIEITFYNALRYNDVDFKNSLKSGVF
                     ANMKMELLPKVGLKLTLPLKKETAINCDLGADAKAFKITLKGEKTTYILPKRQISISG
                     EHKVMIDPGHGGADYGAIRAGINEKDITLDISKRVESILREKGVTVLMTRVKDDTVSL
                     KDRTFICCENNPDIFVSIHVNSSTKPEITGIETHYYQPQSVGLAQTIHSSMMSLIKSP
                     DRGLFKSRFYVINHTEVPAILVEIGFLSNDAERAQLVSEQRKQATAQAIAEGILKYLN
                     QGKK"
     gene            45605..45928
                     /locus_tag="PKI94_01395"
     CDS             45605..45928
                     /locus_tag="PKI94_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25426.1"
                     /translation="MNKRWYDNHDETLQTFDILKKLDRESRKRLANDIVDVANQIKAV
                     HREEDEPSLTIGLERVLGIWQSSNARRWYDKQEDLNTAIKTISTLPEDDFLNIMEGIC
                     VSLKE"
     gene            45953..46372
                     /locus_tag="PKI94_01400"
     CDS             45953..46372
                     /locus_tag="PKI94_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25427.1"
                     /translation="MKIQRVTGLQAANKLSKSPNFNGNLYLQKGIIDSSLEFSKKNLH
                     SLFDQIIPKDFFETFYKMINNLSKRISKKDELPNVVFTIYPKKEGLIIEGLRGVSNRN
                     NRKTVEFLPQESFRKRLNDAFLAIKKYYDDFDKAVKS"
     gene            46482..46868
                     /locus_tag="PKI94_01405"
     CDS             46482..46868
                     /locus_tag="PKI94_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25428.1"
                     /translation="MQINRVSNSTSFDGRLLIPSHKKAGEMVKYTERGLRALLGDNVC
                     KYDCQEYLSSLNNLLRIVKKESKNFILSLYPVPENGNVRFIFSQDAKFSKVVEQPAND
                     LKIVGEEIAVKYKTIMQDPKQFSELA"
     gene            46998..47168
                     /locus_tag="PKI94_01410"
     CDS             46998..47168
                     /locus_tag="PKI94_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25429.1"
                     /translation="MAKIIENKVGRRTIRLSVDDVISTVREYQRIVRNPKTYEGIRIL
                     LKDSSIYLPEDI"
     gene            47262..47654
                     /locus_tag="PKI94_01415"
     CDS             47262..47654
                     /locus_tag="PKI94_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNW25430.1"
                     /translation="MKNFSIIALIFVIPLVAYMILTKPDASFANKAGGGVKAQVIKFA
                     SPMCLDCKKMDKILKELHPKYEGSINFIEIPVQNDDEYTKGQIEKYNVTLVPTMIFVN
                     TKGQKVKKIEGYIDKDELEKIMKDLING"
     gene            47647..48360
                     /locus_tag="PKI94_01420"
     CDS             47647..48360
                     /locus_tag="PKI94_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNW25431.1"
                     /translation="MDNYISLFSQSAGGGEHFQLILLAVSFLGGLLASISPCSLAMLP
                     IIIGYIGGYSKESPIKIFVQMMFFVFGTSLVFATIGVICAVTGKVFISFAPGYFILIL
                     ASLLMVMGLNILGILDFNLPVIIKQMPQSNGRNMFLYPILLGAVFAIAGTPCSTPILA
                     GIMAFASLSTNIALAVLMLFLFSLGQGLILIIAGVFTSTLKGFKSFAVVSEILLRLSG
                     ILLLGSSLYIFYKVFAPLL"
     gene            48487..48951
                     /gene="ribE"
                     /locus_tag="PKI94_01425"
     CDS             48487..48951
                     /gene="ribE"
                     /locus_tag="PKI94_01425"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNW25432.1"
                     /translation="MAKTFEGKLTVKNEKFCIIVSRFNEFISSKLLSGAVDELSRHGV
                     KDENISVIWSPGAFEIPLLAKKCAKTGKYDAIITLGAIIKGATAHFDYVSAELSKGIA
                     SVSLETGVPVIFGVLTTDNIEQAIERAGTKSGNKGSDAAKAAIEMANLVQLI"
     gene            48976..49851
                     /gene="murQ"
                     /locus_tag="PKI94_01430"
     CDS             48976..49851
                     /gene="murQ"
                     /locus_tag="PKI94_01430"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNW25433.1"
                     /translation="MSEVITETRNLLTRDIDIISTREMVQKMNDEDKLVAFAVEEALD
                     EISQAIDIISKQFLKGGKLYYFGAGTSGRLGVLDASECPPTFSVEPDMVQGIMAGGDS
                     AFKTAVEGIEDNFDAGVEDAKILTEKDVAVAISASGNPNYLLGVLAQAEKVGAKTVGI
                     TCNPSGKISEEAGLVICVDVGPEVIAGSSRLKAGTAQKMILNMLTTGAMIKIGKTYEN
                     FMIDLKASNEKLKDRAIRIVAELAQVTHAEALKKLIEFNWEIKPAIVSLYLAIDIDKA
                     KEQLKKHNGILRKVI"
     gene            complement(49848..50156)
                     /locus_tag="PKI94_01435"
     CDS             complement(49848..50156)
                     /locus_tag="PKI94_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25434.1"
                     /translation="MINDINFCSNQPFVKYRPHHGAFNPKDVHPMDIVRVDNYEKAMN
                     GEQNHYFSAIALTYPDRKGNVKVLDLKNPYSPEVKNINIYNKFDDKIEAKLGNLDLIA
                     "
     gene            complement(50178..50477)
                     /locus_tag="PKI94_01440"
     CDS             complement(50178..50477)
                     /locus_tag="PKI94_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25435.1"
                     /translation="MNLNVSSVSFKSRPQKIDFVKSSENSNEFNSSDVVDFEYKPLTA
                     RGINEYIPGIILSKDGNEAEVAILKGRDKGEIVHIDLNNSDYRPVNVLGSLDITA"
     gene            50718..51719
                     /locus_tag="PKI94_01445"
     CDS             50718..51719
                     /locus_tag="PKI94_01445"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNW25436.1"
                     /translation="MKLTVLGPGCWGLTLAWLLTNNFDKITVWGRQEDLYEDLIKNKH
                     CSKPLEIQLDEKIEITSDLSSAIKDSEIILLVVATSGIRPVCEALKKSGIKKEQILVN
                     ASKGIELPSLMRMSEVIKEVLPEQKLAILSGPTLAKEVLLGKPTAASVACENLETAKF
                     VQQACNVSGRFRLYTNKDVIGVELGGSLKNVIAIASGFAHAMNLGDNCMGALLTRGMA
                     EIVRVSVKLGANPSTLYGLSGMGDLIATCSSPLSRNYTVGAMLGKGKKIDEILSELGS
                     VAEGVKTSKAICELAKKLDIEVPVSEAIYDAVYTDITPIEVLGKLMNRKLKEEESYL"
     gene            51732..52646
                     /gene="ricT"
                     /locus_tag="PKI94_01450"
     CDS             51732..52646
                     /gene="ricT"
                     /locus_tag="PKI94_01450"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNW25437.1"
                     /translation="MKYAVRLLKNTFHPLQVPETLELEHGQMVLVRTEKGEEALKTFL
                     VNPEIAQQWEKSKPEALPVIRTLSQRDMQTLDEIKQEEVESFNKCKELVKKHNLSMNL
                     VQCKITFDRRKITFYYTASERVDFRELLKDLTQTFKRVRIDLRHIGVRDETSIVEGNG
                     ICGRPFCCCSFLRKFDSINVKLAKDQGMPITPGKISGTCGRLLCCLNYEYSNYIEAAK
                     DMPPVGSSVMTPDGLGKVFSLNFLNSKLSVKLEDGKIKEYKKAELEMVDAEVNIEIDI
                     PVVAYPEETEHVDISQLEDDKNSSTGNV"
     gene            complement(52711..53214)
                     /locus_tag="PKI94_01455"
     CDS             complement(52711..53214)
                     /locus_tag="PKI94_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25438.1"
                     /translation="MSLNVSYNQSINRGIDSKALVDVTQAIFKRAESKSSELSNLDFS
                     KFKKPDLGLDLYAGKVNAATAREISMASSGVQVSLSEKAVKSLQFLNSEASKAIFKNV
                     EGKIALPVEEKGNETKNVYQLPKFTELVKPATDLNKDKNNSNPFYKGELRKAEKKETE
                     EKINIFA"
     gene            complement(53411..53650)
                     /locus_tag="PKI94_01460"
     CDS             complement(53411..53650)
                     /locus_tag="PKI94_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25439.1"
                     /translation="MKVMFNCCPRPSVYTLFRGEGKKPETTTVVAQTTAAAPAAEQSK
                     TDTVQITTKTTEKAPEVQPKQETEPKKCEGPDCKK"
     gene            53825..53974
                     /locus_tag="PKI94_01465"
     CDS             53825..53974
                     /locus_tag="PKI94_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25440.1"
                     /translation="MNMQVNDSFVQETFDIRKLWNEQHPIIHTWFLLSSVSICFLSAI
                     LFIIP"
     gene            54063..54863
                     /locus_tag="PKI94_01470"
     CDS             54063..54863
                     /locus_tag="PKI94_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25441.1"
                     /translation="MKRAVILLVFYLFGTNSGFCDVTYFPPLQPLGTNANNAFALRNY
                     NQVNNADYQKITQIENSLYGKNFAYQDILIRLSRIEKNIFNRTYPNLSLSQRVDNIVA
                     NYNQTTQYPNISKNTLSKMEMKIFGQNYNGDSPEVRIERLEQQLLGAVQSGDITTRYT
                     TLKTLAGNYNSNQYSQNYYSNPIVGTQGGWKGVLGSLGGMLLGGNGMMTGFTPPIDQY
                     YGYSNPSYLNYSSLGNPGYGYYNGYRTNHGYYDRFNNYGSQTGVTILD"
     gene            complement(54913..55518)
                     /locus_tag="PKI94_01475"
     CDS             complement(54913..55518)
                     /locus_tag="PKI94_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25442.1"
                     /translation="MNMQQSGIYELLKLLEIVLEKSFPILPNYLVAIKVKEVESLIDR
                     IYASLPTEVQEARAFLRRREELQIEAQQKAEKIIFDAQNEAGRLLSESDVLRAVQREA
                     EKIKEQVISECEDIKRKALSEAEAIRVQAADEAIRIREGAELYAEQVLTNIEHDLIQL
                     QQVVKNGQVYMEKLRSESLKNFDTLKDNYPQNQAMEDFKIG"
     gene            complement(55561..56040)
                     /gene="coaD"
                     /locus_tag="PKI94_01480"
     CDS             complement(55561..56040)
                     /gene="coaD"
                     /locus_tag="PKI94_01480"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNW25443.1"
                     /translation="MTIAVYPGSFDPITNGHLDVLKTASEIFEKVIIAVSKNSEKKSL
                     FTFEQRLDLIKKSINGLNNVEVDSFDGLTVEYAKSKGAKVIIRGLRAVSDFEFEMQLS
                     QTNRSLAGDIKTVFLTTRPKYNFISSSTVKEIARMHGDVSKFVTEPVEEFLKLRFAK"
     gene            56284..57528
                     /locus_tag="PKI94_01485"
     CDS             56284..57528
                     /locus_tag="PKI94_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25444.1"
                     /translation="MVNSVSFGNSYGNFQPQGYVSPYYLKKAEDTAKYTTAAVNAQSN
                     EGGPFTGVGLWSGFMGAGWLWRNRKDIRGGFNTLKVNGQTQAELFKADGGLWSRNAYK
                     QAFLRYETEAAKAAKLSSVDQSIKNLNKLSAQAKKAGVTFEEYVAKNAGKTGMLSKAG
                     DFIKNTKAVKAAGKIPGLSKIAKLGKGNGFFFAISAALELFTQVIPTFKQLGAKAGWK
                     QIGKSTATTIGTVGGFVAGQAAGAAAGAAIGAAVGSVIPVAGTAVGAVVGLACGFLGS
                     WLGGKAAKAVVGENELDKAKKEGRLPADDAQPEVAAQTQLPGQNESVGQIAQLPPIPG
                     LENPLMENNSMPPFTAMPQPQAQQQPQQQVTAFGVNNPFAARRQKQFQPNSNPFGAGI
                     QNQMSSLPYAERDFMSMTAGLA"
     gene            57651..58334
                     /locus_tag="PKI94_01490"
     CDS             57651..58334
                     /locus_tag="PKI94_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25445.1"
                     /translation="MKTKILILLLSVMFGVNSVYAAPFKADAKTKRIPAGTKLKIELM
                     DSLYTQVGNQGDAFNAMLLEDQKSQTGVILPAGSIIRGSINKVTPIKMLSRGAVLYLD
                     FDHVVTPNGRQLPLSMGVCNRAKITDDGGLYESKGYGEALQKNWQKTVEITQNVTQFG
                     IDAGENSFNGAVFITAPVCAIGGAMGGGAYLLGDSIIDLFRKGSDVKLNKGTVLDVML
                     TQPIDVPVN"
     gene            complement(58555..58875)
                     /locus_tag="PKI94_01495"
     CDS             complement(58555..58875)
                     /locus_tag="PKI94_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNW25446.1"
                     /translation="MKQYYVYILTNYNRTVFYTGVTSDLIKRAYEHNNESADGFTKKY
                     HIKQLLFYEIHYDILEAISREKTIKRWKRAWKWNLIKDVNPNLVNLYDNGNILPLPLL
                     TTVY"
     gene            complement(59104..59964)
                     /locus_tag="PKI94_01500"
     CDS             complement(59104..59964)
                     /locus_tag="PKI94_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNW25447.1"
                     /translation="MAITVNTNLSAINIQNNLNDATDAMSKSMLRMSTGFRINSASDD
                     AAGLAVSTSFETTLSSSKVASKNVQIGSNLLTTTEGTLGVIKSNLQRIRDLAEQSANG
                     TYSADDRAAMKAEAQQRALEIDRLANSTSFNGIKLLDGTQSSGIAIQVGTGSSADDSL
                     TISSDVFAKATVSAIQLTPAGGTSIDSAFESAASSKAFLANCTTALTNITNRQTKIGA
                     FQNRLSAAGKNLTVQQTNLTAANSTIKDADVAEESANYVKNQILQSASASLLAAANQA
                     PSIALKLING"
     gene            complement(60519..61535)
                     /locus_tag="PKI94_01505"
     CDS             complement(60519..61535)
                     /locus_tag="PKI94_01505"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNW25448.1"
                     /translation="MNKPNIAILGATGVVGREILKVMEELDIPFNNLKLLSSARSAGK
                     EMEFKGKIYKVEEAKPESFEEVNICLASAGGSTSLSLAPEAVKKGCVFIDNSSAFRMD
                     KDIPLVIVGVNDEDLKLHKGIIANPNCSTSQLMLVLKPLDDFAKIKRLIVSTYQSVSG
                     AGIAAINELTENTKAALDEKTFENKVFKKPIAFNVIPQIDSFLENGYTKEEMKVVNET
                     KKILHLSQSLPITCTAVRVPVYRGHSEAVTIEFENEITPKKAKELLKNAYGIEVIDNI
                     ENYEYPTPIDAAYKNPVYVGRIRKDLAFENAISLWCVADNLLIGAALNTVRIAQKVIS
                     MNLY"
     gene            complement(61726..62484)
                     /gene="dapB"
                     /locus_tag="PKI94_01510"
     CDS             complement(61726..62484)
                     /gene="dapB"
                     /locus_tag="PKI94_01510"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNW25449.1"
                     /translation="MMKIAVCGACGKMGKEVIKAVGENPEMKLVAKIDICGGESVFKS
                     IEDAIKSVEIDVLVDFTQPKSIFDNAKFCLNNKIRPVIGTTGLSDAQIEELKKISKEK
                     NVSCLIAPNFSTGAVLMMMFSQQAAKYFDNAEIIELHHNQKKDAPSGTAIKTAQMIAG
                     VNENKTFATGNCEEVELIKGSRGGVAYSDIHIHSVRMPGYIASQEVIFGASGQIFKLR
                     HDTMDRACYMQGVILAIKHVAQNSDFVYGLENIL"
     gene            62547..63458
                     /gene="rnhC"
                     /locus_tag="PKI94_01515"
     CDS             62547..63458
                     /gene="rnhC"
                     /locus_tag="PKI94_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNW25450.1"
                     /translation="MNTFSQKIPIAKQEAIKKFILQNFQAIVHPKQYTFWEIKNKDFV
                     VTFYNSGKFVIQGKNIDILLDVLSSKFHEFSEQEIVNGKHNTMATPLTVHSSPLTYIG
                     TDESGKGDFFGPLVIAGVLIDEKNRPIFKDLGIKDSKMLKDENILKMAKQIQKNAVFS
                     TVVISNAKYNELYKEFKNLNKLLAWGHARVIENILEKRPCEYALSDKFGDESLIKNAL
                     MKKGKVITLEQRVRAESDIAVAAASVLARATFVEKMQSMEKFYGIKFPKGASEKVKDS
                     AKEFSRKYGRDRLKEVCKIHFKTYKEL"
     gene            63519..63605
                     /locus_tag="PKI94_01520"
     tRNA            63519..63605
                     /locus_tag="PKI94_01520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63553..63555,aa:Leu,seq:taa)
     gene            64039..64115
                     /locus_tag="PKI94_01525"
     tRNA            64039..64115
                     /locus_tag="PKI94_01525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64073..64075,aa:Met,seq:cat)
BASE COUNT        20546 a        10938 c        12170 g        20465 t
ORIGIN      
        1 tgttaatggt ttctctttca agtcccgaac ctcggtgttt aagggttttt gcttttacaa
       61 gaagttcatc tgttattccg tttataaccg cttcgttaaa taattttgct atatcgttac
      121 agtttttggc agaatcttca aaacaatcgt aaaagacctt gttctccggc ggtaataatc
      181 ttgaaaatat attaaatttc actttatatc tcccaatttt tgctacacac acccatgata
      241 tgtcaaacaa aatctaaagt aactatcatt ttcttttttg ttaccaaata ttaaactcaa
      301 atttaaagcg gcgggaattt tataaatttc acgccgcttt tttaatattc aaaatgttct
      361 tttgtttgca gatattctcg aatttcattc aaagattgct gaacaatcct tgtaatcctt
      421 gaggaagaaa gtccgacttg tcttgcgata tctttaacct gcatgtttct gtaaaatctg
      481 tattcaacaa ttgttctgtc tttttgggga agtgtagaaa ttgcttctct aatcattctt
      541 cccatctcgg aaatttctgc tttttcatcg ggcattgcag aagtgtcttt tacaaaatca
      601 aagggcgaat cagtatctga agcagcagca gcaaggctgt ctatggataa aattgtttca
      661 taaattgctt ctctgacttt tgtcggtgaa acatcaagtc cttcttcatc actttcttcg
      721 ccgtgagaag tttctccaat atcttcttct gttttgttgt gtttaagaga ataccacttg
      781 tagcggtttc taagttcgtt tcttattgcc catcttacgg cagtacctat gtatgcagcg
      841 ttatattttt cgagctgttc aggggtttta tttttaatca taatttgcac cgcaataatg
      901 ccgatactta ccagctcttc gtattcaacc atatgcgaag gaattcgacg atgttcaacc
      961 tttgcaacct tttgaatgat gtctatgtat tctttaattt tagattggtt gtcatgcata
     1021 acttttatta attactcgtg tcgggtttgt ccattattct accttgattt tgattatttt
     1081 tcaagttgta aacaaaaagt aaaatttcag cgaccgcttt ataaagctcg ggcgggatta
     1141 tctggttgat ttcaaccact ctgaaaattg ctcttgcaac cggaggatta tcaattaccg
     1201 gaaccccgtt ttctgttgct atgtttataa ttttttttgc aataagctct gttccttttg
     1261 ccacaagcat cggtgattcc atttcttccg cgtcatattt aagggcgcag gcaatgtggg
     1321 tagggtttct tactacaaaa tcggcagtcg gaacagaatc catcatactt tgctgaagca
     1381 tttgttggcg gcgttgacga agggcagctt taacgtttgg atcaccttct gtgtttttgt
     1441 attcgtcttt aatttcttta aaggacattt tttgatcttt cataaatttc caacgggtga
     1501 ccatataatc ggcaccggat atggcaagaa aggcaataca ggctttgaat acaaattcta
     1561 caataagttt tccgaattcc atcataaccg caaagttgtt atcaatcgcc gcaagtctta
     1621 gaatatccgt aatgtgtgca gaataaacac tccagcctat ccagcccaga attattactt
     1681 tcaaaatgtt ttttaaaagt tcaaaaagag ttttgactga aattaagttt ttaaaatatt
     1741 tagaagggtt aagtttatca agtttcggga caaggggtgc tgttgccaca agaggtccta
     1801 cttgaataaa atctgccata attgccacaa accatgctaa aaacaaaata ggacctatga
     1861 ttaacaaggt aggaactatt gcaacagtta aaacataagg caagcctata tcgctgaggt
     1921 gtttattggg tatttgctca taacagagca caaacagctt tgttatctgg tcccaaataa
     1981 aaggcgaaag ggcataaatc gctgtaaaca aaagcaataa agatattgcg gttgaaaaat
     2041 cttttgattt tacaacctga ccttcttttc ttgcctgctc caattttcgg ggcgtggctt
     2101 caaattgttt gtcttcatct gccatggtct aagcctttta gttgtgttat taattttatg
     2161 tttggagtaa ataggaaata gtaaatagtg aatagatttt tgcttctatt taccatttac
     2221 tatttaccat ttactaaatt atacaatgat atgttagcat aattatttaa ctatctccac
     2281 gccggcactt gtgcctaacc ttgttgcgcc tgcttttatc atttttatag ccgcttcttt
     2341 atccctgatg cctgcagagg ctttgacttc aagcccgaag ggcgaaatgg ttttgtacat
     2401 aagttcaaca tcttcaactt ttgcacctac tccgtttttg acaaatccgg ttgaagtttt
     2461 tacaaaatca gcttttgctt ttatacagat ttcgcaagct tttttgattt cttctttggt
     2521 aagcaaatcc gtttccaaaa taacttttaa ttttttcttc ccgcaggctt ttttaaccgt
     2581 tgcgacatct tcttgaactg ttttgtagtc tttgtttttc atcgcagaaa catttaatac
     2641 catatcaatt tcatctgcgc cgtcttttat ggcaagtttt gtttcataag cttttgcctc
     2701 gcttttgctt gctcccaaag gaaatccgac taccgcaact atttttacac cggaattttt
     2761 taagtttgct tttgcaagtt ttacgttaac aggattaacg caaattccca aaaatttgta
     2821 tttaatggct tctttaaata aatttgccag ttcttttttt gtagcatctt gttttaaaag
     2881 tgtgtgttca atatatctgt ttaaattttt catacatata tcctccgtaa agctatttta
     2941 taacaaaaaa tcgggcaatt atttatatgt ttaattttat taagataatg ctttaaaatg
     3001 tcaacttttt ttgtcagttt gtttttaata ctatataaaa gtgtgttttt taataaggtt
     3061 ttcaagtgaa gataaatccg attataagtt ttaaatcaaa tatcgaggcg ttaaagacgc
     3121 cgcctcacaa tgcagttgca acaaatccca ttcaaaactc gcaggatttg caggctactg
     3181 cgcttgatta taaagtaaaa acacctatga aatatagcga tttaggagaa cttgagcttc
     3241 catgttctac aaaaggacac ctttacaagc ttgaaaacgg acagagagtt gttataataa
     3301 caaaaatagg gccgacagta gtcaaaactt atgttaattc aggttctatg aacgagcctg
     3361 ataaattcag aggcataagc cattttattg aacacaatct ttttaacggc tctgaaggct
     3421 taagtgccgg tcaatttttt gacaccgtaa ataaaatggg tgcaaacacc aatgcctcaa
     3481 ctgggtttgc cgctaccgat tattatatcg aatcaaacct tttaaagaaa agcgatttgg
     3541 aaaaagagat taaaatacac gcttctatgc ttgaaaaacc gacttttgcg cttgatatga
     3601 ttgagaaaga aaaagggcct gtaacatctg agattaatat gattttggat gatcccgaaa
     3661 atatcgcaac aaataatact ttaaaaatgc tttttaacat aaactccact tccgaagaca
     3721 taatcggggg caccgttgac aacattaaaa aaattaccag agaagatgta attgactatt
     3781 ataagaaaaa ttattcccca cagaatatgg tcaccgttat aaccggtgaa gccgaaccgc
     3841 aagaagcaat agggcttgtt tcaaaatatt tttcttcaaa caacacaaac ccaaaaccca
     3901 gaaattatga agatttaaaa ccgatacaaa aagccgtaag aagggacata atctctgata
     3961 aaacccctgc ttcaataata agcataggct ttgtaggacc taaaaatatt gacacaaaag
     4021 acaaaatttt acttgatgcc tttcaatttt ttatgatagg ttcaaatgtt tcaaggctca
     4081 ataaatctct tgaaagaatt cagacaaacg cacttatgac atcagacagg ataagcacaa
     4141 gacctcaaga taacacggtt atactttttt caacacaagt gtctgaagag aattcagaaa
     4201 aagcaattca gatgattttt agcgaaattg ccaatcttga aaatgacccg ccaagcgaaa
     4261 aagagttaaa tatagttaaa aagaaattaa agcttaacct ggcgcaagtt tttgaaaatt
     4321 ctcagcttat aaacacagcg gtcggtacaa gtatgcttga caatgacctc aaaagtgtta
     4381 tggaatttga aaaagttatc gacaatatga catcatcgga tatagtcaat tttgcaaaaa
     4441 attattttaa tcttaacaaa gcggcaatga ctgttgttca tcccaatacg gttaatgctg
     4501 aggctataaa acaaaattat caaagaactc acaatgtttc atttaccgga aatttagatg
     4561 agattaacca caaaacagca cttgatttaa gcaaggttaa aagatacgga atcacaaata
     4621 atttttcggt ggcgacaaat gaaattcata ataaccttgc gacttttgat ttaagcttgt
     4681 ctgcagaaaa gcctgcagac attaagccgg gtgtttctaa tattttggcg ataatgttaa
     4741 acaggggctc aaaattcaaa aacgaaaaag atttcttttc cgatttggaa aatcaaggtg
     4801 tttccaccca gttttatgca agtgaaagag gtataggcgt aacttcaatg tttttgcctc
     4861 aagatggcaa gcttgcgatg aaaagcgcaa aagaagtaat tttaaacccc aggttcaagc
     4921 aggaagaact tgactatgca aaaaatattt tgcgcgaaaa cataaaaaac acccctaaaa
     4981 tttctatgga cgggttaatg aaggaaatgt ttaaaggtca attttacggt aacagcctca
     5041 gcgatatttt aaataattta gacggtatcc aaatggcaga tgttatgtcg ctttataatt
     5101 atattgttca aaatgctcaa ggaacagtcg caatttctgc tccttttgaa aaaaatcctg
     5161 atttaaaaag tgaaattttc tctgaacttt gctctgattt tccgacactt aagccttctg
     5221 taccttcgct ttttaacgga tttattcctg taagagacaa aagcgttgta gttcaggaac
     5281 acaataaaag tcaggcagaa atccaaatgg gctacaaatt tgcggtcaac ggaaatctca
     5341 aagacagtat cgtttttgaa cttatgaatt ctattcttgg cggaacacct tcttcaagac
     5401 tattttctga tttaagagaa aaacaaaaac ttgcttatca ggtaaattca agatggacaa
     5461 atatggacaa ttccggaatt ttgtctttgt atattaaaac aacgacagac gatcctcaaa
     5521 gcggtcaaat ctgctatgac aatcttcaaa aatctttgga aggttttaat ttccatattc
     5581 aaaaaatgaa aaatgaaaac gtgacagaag acgagcttga aagtgccaaa ttaactttga
     5641 aaaataaaat cttgaacgca tcagaaatga catcggataa aaattcctcg cttttgagcg
     5701 gattgaattc tttttacggg ctttccaaaa ataatcagct gcttgatatg atagacagca
     5761 ttagtgcaga tgatattaaa aaatgcgcaa attatgtatt caattcaaac ccgactgttt
     5821 ctgttgtggc gacaaaaaat actattgaaa ataacaaaga ttatttagct tctttgggca
     5881 atattgtaag aatgtaatat tcttgaactg cttgaaaaat tgggctataa tgctttttat
     5941 gaaaaacaat tctttattcg atgtaatttc gcctgtaatg gtaggacctt ccagctctca
     6001 tacggcaggt gctgtgaggt tggggctttt ggcaagaaat atttacgcgc agcctgtaaa
     6061 aaaaataacc ttcaggcttt ataattcttt tgcgcagacc ggaaaagggc atggcacaga
     6121 caaaggactc atggcaggac tcttgggttt tcaggttgat gatgtgagga taaaaaaagt
     6181 ttttgaactg ccggaagcaa aagaaatcga gtacgatttt gaatatttgg aagattttaa
     6241 tcgtcatccg aatgctgttg attttatttt tggcggaaaa gataaaatgg aaatttttgc
     6301 agaatcagtc ggtgcgggaa atgtcagggt gcgaagaata aataaattcg gagtagatat
     6361 aaccggtgaa tatgacactt tggttttgat ttataaagac aagccgggca tgctttctaa
     6421 agtgggcgga attattcaag gcgaaaaaat caatattgct tctttgattt gcgacagaag
     6481 tgccaagggc gaggatgctt caatgtgtat tgcgcttgac agcgggctta attacagtgt
     6541 tgttcagaaa attaaaaaca ttgaagatgt ctattttgta agacatgtga gtaaaatatt
     6601 atgagaaaag aaataacgac ttttgccgaa ttactttatg cttgcggtca aaagaaaaaa
     6661 agaatatacg aactggttgt tcaaagggaa gcttttttac ttgatttatc agaagatgac
     6721 gtgagaaaaa aagtaaaagt atcgcttgat gcaatgaaag aggcgataaa acacgggtta
     6781 aaatcagacg aactttctat aagcgcaaaa tgcggagcag attgtaaaaa agttttggaa
     6841 aaattttcta aaaaaccttc gcttttcggc aatttatttg aaaaaattac cgcttacgca
     6901 cttgcaacgg ttgaagaaaa cctgcgtatg ggaaaaattg tcgcatgccc tacggcgggc
     6961 tcttgcggaa ttgttccttc tgttctggtt gcggtgtcag aggaattaaa actcacggaa
     7021 gaagaacaga ttaacgcact tataatagca ggtgaaatag gctcaattgt cgcaaacaaa
     7081 gtagcacttg cgggggctgt tgccggctgt caggcagaat gtggcgtggc agcagctatg
     7141 agtgcagggg ctttggcata tcttttggga gcaagtgaaa ggcagattgt tcatgcggtc
     7201 gcacttgcgc ttaaaaatgt tttggggttg acttgtgatc ctgtagccgg acttgttgag
     7261 attccttgcg tgaaaagaaa cgtttttctt gcaatacatt cggttgttgc agtagaactt
     7321 gcgcttgcag gggttgagag caaaatcccc gccgacgaag tcgttgatgc cctgcttcag
     7381 acaggacagt tgatgtcgcc tatgcttaaa gaaagttctc aggcaggact tgcaaagaca
     7441 aaaaccgcca aaaaaatcga acaaaaatta aataaaaaat atcagaattt ataaagggga
     7501 cgcggaaaaa ttttaatttt tctgcaagtc aaacttattc tgcacgttca aaaaatgcag
     7561 ctatctgttt gtgaggaatt gtttttgaaa tagggcgacc gtgcgggcag gtgtaaggtt
     7621 tttcgcaagt ttgccaattt tgtattattt cctgcatttg ccacatatta agtttttgcc
     7681 ctgcttttat agaggccttg caggacatgg aaataaggat tttttcttcc aatccgtcca
     7741 aatctccttg caggttttcc agaatatctg ccaaaaggtc ttttggatta gcttttgcaa
     7801 gaagttgggg aactttttta aatgtaagtt gattatcttt taaaaattcg attccgtatc
     7861 caaatttttc aaatttttct ttattttctt tgattaattg ggcttcgctt gcagaaactt
     7921 ccaacacatc tgaaataaat aaaagttgtg aagccgcctg tttctgtttt tttaattttt
     7981 cgtaaataaa tctttcctct gcgatgtgtt gatcaacaat ttcaagccct tcttcttttt
     8041 cgattagaat gtaggtgttt ttgtactgac cgatgattgt gggtgaacag tgagcagtga
     8101 gcagtgagct acaaggcgag gagtaaagag tgaagggtga aaagttatcc tcttcgcccc
     8161 ttgcgggaga ggggaaattt gcaacgtgag aggagttatc ctcgaatgtt tgcaaattgg
     8221 gtgaggggta aacaggcaaa attgcttgtt caaaggcctc ttcttttaca taaacatcgt
     8281 cgctttcttc ttttttaggg aaggcgaagg caacattttc aggtgaagcc gcctttattt
     8341 catttctttg tccgaaatct ccccctgatg aaagtgccat gtttattgaa gattgaataa
     8401 atgcaaaaac ttggtttgca tttttgtatc ggacttcttt ttttgtgggg tgtacattaa
     8461 catcaatctc agaagggtta acttctaaat taagcaccac aaaaggatat tttcctattc
     8521 cgattaaatt tttgtaagcc atatcgattg attttaagaa aaccggacat tttatcattc
     8581 ttgaatttat ataagtgtgg taattttttt tgcttgaacg ggtaaaatca ggagtgctca
     8641 caacccctga tattttaagc cctgaaaggt tgtctgtttt tataacctct tttaagttat
     8701 cgataacttc ttgtgaataa acttctttta ttacttcttt taaatcgcct aatcctgatg
     8761 tttttaaaat cgtttttccg ttgtttttaa gctcgaacga aatttggggg ttaataatgg
     8821 caagtgcttg aattaattct tgaatataag aaaattccgt tttgggattt tttaaaaatt
     8881 ttaagcgcgc aggaagattg tagaaaaggt ttttgacttc cataatcgtg ccaacagcac
     8941 agcctgtttg aacctttttt acctgcgagt tttcgcattc gacttttgtt ccgacttcaa
     9001 aatcttttgt tcttgtcgtg caggttaatt ttgcaatcga aattatactc gaaagggctt
     9061 cgcctctgaa cccgagcgtt gaaatagcaa acaaatcttc gtcggtttcg attttgcttg
     9121 tggcatgttt tgaaaacgca agcaggatat catcggggtg aattcccgag ccgttgtcgg
     9181 caattcttaa atccctgcat tcgtttgaga cttcaacaga aatttttgtc gcactcgcat
     9241 cgattgaatt ttccacaagt tctttaacaa cagaacaagg gcgttcaatt acttcgcctg
     9301 ctgcgatttg gtttataaga tgtttagata actgtttaat tcgtttcatt ttatcttttt
     9361 catttacccc ctcatacaaa aattgtacat taacagctaa ttttcttcaa atatcaatta
     9421 aattaatgat tacaatctta acatattttt ttatacttct attatggcaa gatatagcac
     9481 aaaaagtaat ttagcaccca aaaaagtcga aaagcattta atccccgact taaaagtcgt
     9541 tgacaaaaat gcgataaaaa cgactgctta cagcgacatt aatcttaaaa agtggcgtga
     9601 ttattcgcat ataaaaactg atactctttg ggagtttcct tcaagattga aagaaggagg
     9661 acattctaac gaatatcacg gaaattatat tcctcaaatc gctcaacagc tttatgaaag
     9721 atttactaaa aaaggcgatg tcatactgga tttgtttttg ggttccggca cctcgggtat
     9781 tgaagccgtt aatatgaaaa gacgctgcat cggcgttgaa ttaaaagaag aattggcgca
     9841 aaaagtgtca gaaaaattca gcgaaaagca gcttgtaact gatgtgaaca taatttgtgc
     9901 cgattcaact tcaaaagaag ctaaagaaaa aattcaggca agacttgata ttatgggcaa
     9961 aaaacaggcg cagtttcttg tacttcaccc gccttatgac gatataataa agttttccga
    10021 taaaaaagaa gatttatcaa attgttcaag caccgaagaa ttttataatc tttttgaaaa
    10081 agttgcaaaa aacggctatg atttgcttga aaaaggacgt tttgcggtat tgataatagg
    10141 ggacagttat aaaaattcac aaattcagcc tttgggattt gagtgtatga gaagaatgaa
    10201 taatttagga tttataacaa aatccattat tgtaaaagac attcagggca acgaaaaagc
    10261 aaaaggcaaa acggcaaacc tttggcgata cagggcttta gcgggcggtt tttacatttt
    10321 taaacatgaa tacgttatga ttatgcaaaa ggtttaaact atttttgcca aataaattga
    10381 aacgagggta aaatagacaa taagtcctac cacgtctatt gcagttgcaa tcattggtcc
    10441 tgatgctact gcagggtcga ttttcatgtt tttgagtgca aaaggagtga aagttcctat
    10501 aactgttgcc attgtcatat tgataagcat tgcaagcccg acgaccaacc ctaagtagat
    10561 attgtctctc catccccatg tgacaagcat tacaagcagc ccaaagactg agcctattaa
    10621 aaatcctata aacgtttctc taaaaatatg cttaaaagct gaacttaaag taacctgtcc
    10681 ggttgaaagt ccgcgcacca taagcgtaat gctttgtgtt ccgatatttc cgcccaaccc
    10741 tgctaaaagt ggcataaata ttgcgattat cggaacggca tgtaaagtcg attcataatg
    10801 gtttccgacc tgtactgcta taaattctcc gataagagtt ataaaaagcc acggagttct
    10861 cgccctgatt gcttttagaa gatttcctga taagatttca tcctcgtcaa caacatcggt
    10921 agaaatacct gaagatgtat agatttcttc tgtggtttct tcttcaacaa tgtcttgggc
    10981 atcgtcccag gtaatcatcc ctttaaggcg gttatataaa tctacgacag gaagagcatt
    11041 gtactgatat ttcataaaaa tatcggcaat atagtcctga taatcatcaa catgaagctt
    11101 aacaatgtct gtaatcatta tttcttcgac tttttgaccg ataggagagg tcaaaagttt
    11161 gcgaagcgaa attaccccta gaaggtgatt ttgtttgtca acgacataaa tataataaag
    11221 ttcaatattg tcttcctgcg ctttttctct gatgtgagaa agcacatctt gaaccgtcat
    11281 atcgacattt accgtcataa caacaggagt catgataccg ccggcggtgt cttcgttata
    11341 ggttaaaagt tctcttacct cagatgaaaa cttgcgaggt aatttgtcaa ggtaagtttc
    11401 gctctcatct tcttccatat ctcccaagac gtctgccgca gagtcgtgag gcatctttga
    11461 aattaccctt gaagcaagtt cttcatcaat gtcacctaaa atttcaactt gaagttgcgg
    11521 tgataagtat tccaacaaat cggcagcttt ttcttcatct aacgttttga agcacaaaag
    11581 cctttcctca ggcttcagtt cagggaaaaa gtccgccaaa tcagcagagt gatagccttc
    11641 cagctcctct ctgagttgct cgaggttttc gtcatctatg atttcgcgaa tgcgctctgt
    11701 tatgttctct acgttaagca tatttccacc ttataaattg attatatatt aaacattttt
    11761 tttgggatat aattattagt tgaaaaggtg aacaggtgaa aaggtgaata gttaatgctt
    11821 aaagattttt ttcttgaaga agttgaaaat gccgttgaaa aagcggtaaa agaaaataaa
    11881 ctgggtgaga tgaaaagcta tgaaaaaggc acgctttctg tcgaaaagcc caaaaattcc
    11941 gagttcggag attttgcgat aaatgtttca gcactggcgc gatttgccag gctgttgcct
    12001 gcgcaaatcg cgcaggaaat cgtttctgct ttaaaacccc aaaatggcac agtaacagtt
    12061 gtgggcggat ttatcaattt taaggcagga gaggagcttt taaaaaaaac aattgaagaa
    12121 ctttttaagc agaaagaaaa ttacggcaag ccaaaaaaaa taaataaaga aaaaatcttg
    12181 cttgagtacg tttcggctaa tcctacaggt ccttttcaca taggacacgg gcgttgggca
    12241 gctatgggaa gtgctttggc taatttgctt aaattttatg ggcatgatgt tacgcaggaa
    12301 ttttacatta acgatgcagg ctctcaaatt gaaaaactgg gaaaatctct tgaaataaga
    12361 attaaacagg aactgggaga aaaaatagat tttccgcaag acgaaaccga aagaaaaaat
    12421 tattataccg gcgattattt aattccgatt gctaaaaaat atttaaccga aaacacccct
    12481 caccccagcc cttacctctc acaaaacgat actcaatcgt tttgttcggc agagtcgcaa
    12541 ggggagaggg agaactatgt ctcttcaccc ttcacccttc acccttcact tttatcagat
    12601 tatgctaaaa aagaaatgtt agcattgcaa aaaaaattat taaaagaatt cagagttgat
    12661 tttgatattt tttactcgga acttgactta cacaattcgg gaaaagtcga tgagacggtt
    12721 gaaaaactta ataaaatggg taaattatat gaaaaagacg gcgcactttg gtttaaatct
    12781 tctgattacg gcgacgacca agacagggtt ataaaaaagg cggacggctc aaatacttat
    12841 ctgaccgcag atattgccta tcaccttgat aaacttgagc gcggatttga cagaatgatt
    12901 aatatatggg gtgcagacca ccatggatat gtggcaaggg ttaaggcttc aatagaagct
    12961 ttgggctata acccagaaag tcttgaagta cttttggggc agcttgtaaa tcttgtaatc
    13021 aacggcgaag aagtccgcat gggcaagcgc aaaaacatgg ttacgcttga tgaattaatc
    13081 gaagaagtcg gagttgatgc gacacgtttt tggatgtgta tgagaaatat cgacaatact
    13141 ttggactttg atattgaact tgcaaaaaca tcttctgatg aaaatccggt tttttacgtt
    13201 caatatgcac atgcaagggc ttgttcgatt ttaagaaatg caattttaga aaaaatcgac
    13261 actcaaacag gtgaaaaaac atcgccgccg atttctcaat cagaacttga cagtttgata
    13321 aaaaactttg ataaaacaat tttaaatccg atttttgaag aaaaagaaac actggagagt
    13381 gcaaaaagat tgattttgaa gcttgaagaa tataaatttt taatcaaaac agcagcagaa
    13441 aatagggcgg tttatgcgct ttgccgttat gctcaggagt tagccggcga tttccactct
    13501 ttttataccg tttcaagagt tatatcagag gacaaagcat taatgaaggc aagattgtcg
    13561 cttgttatag ctgtaaaaac agttcttgca aatgtattaa aacttcttgc tgttagcgcg
    13621 cccgagagta tgtgagtgct atgtaaggtt aaacacaggg gttatttcgc cgactttttc
    13681 caagtgaagg gttaaaatat ttctttgatt ttggaatttg ataagaggat tttcaagcat
    13741 atctttatcc agccatttca gttgttttaa ggcttcaata agcacaatgg agcgtgcttt
    13801 catatcgcag tccattattt ttaccattat accttctttt ttctttaaat ttatcactat
    13861 gcacaagccg cctgcgccta tttttgctat taaatcggga ttggcgcaca ttattgcggt
    13921 gtcaatcctt tcttctccgc ctataaaata aggtttgttt aagaaagcat ttttaatttt
    13981 ttcatatttt ttgtcaaaaa acagatttaa ataacctttg atcatatttt ccaaaggcat
    14041 ggcaaatatc gggactccgc agccgtcttt tgtcgaagta tattcttttt tgacttcaca
    14101 caaatcatat atttttttct taattgcaat ttgaagcggg tggtcaatat tataataatc
    14161 atttacctcc cagcccattt ttttgcagat gctaagcatc attgcatgtt ttcccgagca
    14221 gttgttttgc aggacacttt ctttcaaccc ttcgattaaa agttttttct gagcggacct
    14281 gctaaaggga acatgcgttg aacattttag gttttctttt gatagattta ttttattaag
    14341 cagtccttca atagtttggg tgtgacatag ttctccggca tgggaagcgc agcagacagc
    14401 aatttcttca tccgtcatat taaaagtttt atctatttca taatcaatca aaagcgaggc
    14461 ttgaagaggt tttgcgcagg agcgtaaata aaacgggtaa tttttgtcat taccgatttt
    14521 ttggatgttt ttgccgactt tcacgacaaa gccgagatgt tcctgttcaa tgagtccgtc
    14581 tcttatgtat tcaataagtt ttgcaggtgt gtaattatcc atttttcttt acaaatgcta
    14641 aaattctgtt taattgttct tttaaaaccc tgttttgttc ggtaagtttt tctatttctt
    14701 ctttatgggc ttttgtttga gttaaatccc gtaaaaaatt gtcaggaacg attgaagtat
    14761 caagtataaa tctttttgac gcctcttctt taattaaaag cagtgatgcg gtatcttttt
    14821 cggtaacata acctaaagaa attagaactt ctgccatttt cataggcttt tcggtattta
    14881 aggattcttt ttgcgtgtta atggctcttt ccaactgttc catggaaatt ctttctgccc
    14941 ttttaaaata ttctccggtt ctgaatttcc ccgccataaa tcccgccatt gcgtgaatat
    15001 aaggcgactc aggtgtttta atataatttt gttcaaggca gaaaatataa tattttgcga
    15061 cttcttccat tgtccaaaag ttattcatgg atatttcaag tatgcttaag ccttcgtttg
    15121 cattgcttaa aaaattataa atattatcat ccaacccgct ttttctttcg ttgagctctg
    15181 ttttcccgat aaatgaaagt atcggaatgt gaagatgaaa tatttctttt tcgcttcttt
    15241 tgataaaatc agtgcttaaa gaacttgcca aatctttttc cagatataga taaattattt
    15301 gttttaccca taaaggaaag gataataatt tatccataaa aagcataaac atgttttttt
    15361 tcaaaataaa acctccaaaa gtcttctatt ttttaaatta tatattataa tgtagttata
    15421 aatcaagatt gtaaagttca atttaacttt ggggttttaa atgggtttag acggattttc
    15481 aatgagtaat ttgggtttgc cggaaaggct tacttccgca caaatggcgg aaagagctga
    15541 gcagcttgcc ttggaaggtt cagagtataa agtaaaagat attacgcaac tggcaagtca
    15601 aaacggtgtt caaagaaaag aaaatgaatc gagcgggttt acaccgggct atagcggaag
    15661 ttttcgcaag aaaaaagacg gtgaagaaac gcctgaagaa aaagaaaaaa atcaggaagt
    15721 tttaaatcag gttaattttg aagaaaaaga ttcaaaagat ttttctgtta gaataaaccc
    15781 tgaaacagaa aacatagaac tttacagcct aagaaatcaa aaagtaatag aaacgatagg
    15841 agcagatgat ttaagagatt taatcttaaa gcttgatgat gcttcgggcg tttttgtaaa
    15901 caaaaaaata taaaaggaac aattattatg aacaatcttg ttaatcctta tttaaaacaa
    15961 tatcagaaga atcaagtcga aacggcatct cctgagcagg tactgatttt actctatgac
    16021 ggtgcaatac agtatcttaa caaagcaaaa atcgcaatcg ggatgaatga tattgaacaa
    16081 attcaaaaaa atctgacagg atgtgaaaga attatacttg aatttatgag cactttggac
    16141 ataaaaaacg gcggctcttt ggctcaaaat ctttataagc tttacgatta cctttacaat
    16201 actcttgtag aaataaacgt gagcaaggat gagaaaaaac ttgaggaagt tttaagacac
    16261 ctgacaggtt taagggaaac ctggaataaa gccattgcaa tggctaatgc cgaaagacaa
    16321 agtaacttaa atgattttaa aagagttgat ataaaagacg atcaagatta cgaagaaggc
    16381 gaatattttg accaagatga cgaagaacaa gaagatgaat aagaggctgc tttatgacac
    16441 aattttcaca gttaaaactt ttatataatc aatttttccg gttatctgat gaaataaaat
    16501 ccatgattgt caatgatgaa tttaatgagg ctatttctgt tgttcagcgc aaagacgccc
    16561 ttataaaagc tattatagaa gcaaaaaaaa ctcttgaaat aaaagaagaa gaaaaatctg
    16621 aaatggaagc tttagaaaaa gatttgaaag aaaaagaact tgcaaatata aatttgctca
    16681 aagacttaca gcatgaagtt gcacaacagt tgggtaagac aaacaagagt ttaaaattga
    16741 acagtgctta ttctgcgcaa aatgacgaaa atagcggctc catgcttgat ttttcagaat
    16801 aaaaggaaat aaattttgaa tgaagaattt gtaaaagaga aaaaaaaggc cgcaaaatta
    16861 tcaatagttt caaatagttt tttgatagtt ttgaaatttg ttgccggttt tatctcagga
    16921 agtataggaa taatttctga ggctattcac tcgggaacag atcttttggc ttcgtttata
    16981 gcttattttt ctatttcaaa atcatctcag ccggcagata aagaacatcc ttacgggcat
    17041 gataaatatg aggattttgc cggttttgtg gaagggcttt tgataatttt tgcagctttt
    17101 tatataattc atgaagcggt caagaaaatt ttaaaccccg aattaataga attggatgtc
    17161 gatttagggc ttttaataat gggtatttct ataatcgcaa acttttttgt aagcggatat
    17221 ctttttaaag ttgctcaaaa aactacttct atggccttgc acgcagacgg tgaacatcta
    17281 aggactgatg tttattcgtc tttggcggta ttcttgggat tactttttgt taaagttact
    17341 gataatcccg tttttgatcc cgttgttgca atttgtgtcg ccgtaataat cttttttgcg
    17401 ggttacagga tatgtcgcga ttgtacaagt aatcttttgg atacttcttt gagtgaaaat
    17461 gaaaattctt atataagagg tatcatcgga gattttataa atgaatccga agtcatagaa
    17521 attaaaaata tgagaacaag aaaagcaggc atgaaaaaaa atattgatat tacactttca
    17581 gtaaacggaa ttatgcctat aagccaagct catgaattat gcgatagaat tgaaaaacaa
    17641 atagaagatt caattcaaaa tacagatatt acaattcacg ttgaacccca taaatagtat
    17701 tatcttaaag tattaattca atttattttt ttagtaatta agcataatgt ttaagcttta
    17761 tggttatagt aacttgattt tggtattctt tgagtaccga aaggaggaaa attatgaaac
    17821 actctttagc cataaaaaaa cctgattttg attttgattc ttttaatcaa gaattcaata
    17881 actttctcag agacactctt tttgagactt cttttgcttc tccattgcat ttaaagatga
    17941 cttcaacctg gcgaccggca atagaagttc agcaaaccga gaaggaatat aaagttaagg
    18001 ttcagctccc cggcgttaac aaagaagata ttcacgtgga tttgaacaat gattttatga
    18061 cggtcacggc ggaaattaag gaagaaaaag acaaaaaaga agcaagtaaa atgcatactt
    18121 gtgaaattcg atacgggaag tatgtaagaa caatagcatt tgataccccg ataaaaaccg
    18181 cagacagcag cgcagaatat aaaaacggta ttttgacggt tacaatgcca aaacaaaaaa
    18241 taacaaaaac ccaaacaaaa cgtttaactg ttaaataaaa attccgacac aaaaggggcg
    18301 aagtttttga aagaaacttc gccccaccat tatttttttt aagaccatgg tttaactaaa
    18361 aacttaattg cattaccttc aatgtgtttt tcaagtgcgg attgaacttc ttttaatgat
    18421 atagtttcag ttatgagttt ttcgacaggc acttgtcctg aggaaatcaa gtcaagtgct
    18481 tttttaaagt attttggtgt gtggtgaaag acgctaatca tttttatgtc accgtagtgc
    18541 atttttgtgg tgtcaaaggt tacggttgaa cctgatttac agccgccgaa gaagtgcact
    18601 gttcccccgg gtcttatcat tgaaaaaatt ctttcccaaa ttgatggcag tccgacacat
    18661 tctaccgcaa cgtcaagccc ttttccttct tcggtaaatt ccataaatat tttttcagga
    18721 tttgggtatt tagtcaaatc aacaacttca tctgcttgtg aaaaactttt ggcaagttct
    18781 aattttaaag gatttctgcc tgctgcgatt acttttgcgc ctttgagttt tgcaagtttt
    18841 gcaaacataa gaccaattgg tccgattcct attattccga cagtttgacc gggtttaatt
    18901 tcggttcttt caaccccgtg aacaacatta gccaaaggct cgcaaaaagc ggctttgtca
    18961 aagcttaaat catcgggtaa cttaagcata tttttttgaa caatccttgc gggaacggtt
    19021 atatattctg cgtaagcacc gtttagtaaa tcaagatttt cgcaaaggtt atattcttct
    19081 tttttgcaga aaaagcattt tccgcatggg gccgaatttg ctgcaacaac tctgtctccg
    19141 atttcaaatc cttcaacatt tttgcccagt tgcgctattg ttcctgcaaa ttcgtgtccg
    19201 aatcctgagg gaatttgttt tattaaaaca ggatgaccgc gtttaaaggt ttttacgtca
    19261 gtgccgcatg taagcgcaga ttcgactttt actaatactt cgccttcttc aagcggtttt
    19321 atgcttattt cttcaaatct tatgtcaccg gggccccagt attgtattga tttcattttc
    19381 atataaatca gcctactttt atgtatgctt tcataacttt gttttcgatt gtatcacgaa
    19441 aagctttttc aatatcgcaa aaatcataaa cggttgaaat attttttact ttaacttttg
    19501 cgcttgaaag cagcttcatt gcttctttta aatctttggg tgaaggagaa taacttccaa
    19561 gcacagtcag ttccctgtaa taaatttcgt tgtttgcgta gccgaaattt tttggagtgc
    19621 ttgcaaaaac aacgattttt cctccatctc ttactgcttt tagagcaagg tcaatagtag
    19681 catcggcacc tgccgtcata aatacaatat ccgaatcaag tttttgtgtt tttgaggaat
    19741 gaacggcttt aattttgaat ttttttgcaa aatcaatgcg ttcttgaatt atgtcgcaac
    19801 cgaaaacgtt agctccgtaa gcttttagtg cctgccccat taaaagtcct attgagccca
    19861 agccgacaac aagaaccttt ttccccttat atttaccccc gcctcttttg attgccctga
    19921 tacagcagcc cagcggctca taaaaggaag cttcgatttc ggtcattttt ttgaggattt
    19981 tgaccgctac gttttgtaaa tgttcttcgg aaagataaat atactcgcta aagcctcccg
    20041 ggattatatt tgtttcttta aaatgtttgc acattgaata atttttattt ttacaataat
    20101 tgcatttaaa acaaggaata tgatgtgaac ttactatttt atcaccgatt ttaaaagctg
    20161 tttttgaatt tatttcaaca atttcgccta caatttcatg ccctaaaaca gtcccttctt
    20221 ttataaaatt ttggcggaat tttactatgt cagagccgca aagtccgcag cctgaagttt
    20281 ttattatggc accaaaacca aattttttta aatttggctt tggtgcatcc tttataacta
    20341 ttctattttt tttaatttgt gctgatttca tttgtcatcg gctctatttc tataacgggt
    20401 ccgctcgaag gtcttgagaa aacataattt ttaatttttt taaaatcaaa tttagatgca
    20461 aaaactgttg caaacaaagc tacgagaaca aaaattaaaa aatattcaat tactgtctga
    20521 ccttttttca tatcaaaatt ttatcacaaa aaacatgtat tttattgcaa aaattcataa
    20581 aaaactgtat gatgaagata taatcgttga gggttttaat ggcaagaata agaagattaa
    20641 gatattttga taaatttaag cttaaaaaaa tgatttcttt tttaagttct gatgttataa
    20701 gtcattatac gagcgttttt atgaagtttc cttttaatac gcttcatgaa tttttgcctt
    20761 tgagatttaa atttatgccc gaaacccatg ttttgaccca aaacggcgaa attttaggta
    20821 tgattacagt ttcaccgact cccggcaatc ctttcagact tgcaataaac agactttttt
    20881 tggaacaaaa ttattacaat gcaggcaagc agctaatcga atttgtagtt gcaaaatacg
    20941 gtgcaaaggg cgcaagcgca tttgttgcag cgattgatga atctcacgga gaactcttgc
    21001 agctttttat agaaggttgc ggatttaggc actgttcaag cgaacagctt tggaaaatgt
    21061 cagaatttca cttcacaaaa gaaaatcatg cgtttataag accgttcaaa aattcagacg
    21121 cccagtctgt tgcaatgctt tttaacgatt ctgttattac ccattttaaa cactctatcg
    21181 aaaaaaagaa aaatgagtat caggaccctt tgtttcaggg tttaagaaat atttataaac
    21241 ttaagtatgt aattgaagat gagcatacaa aaacagttaa agcttatttt tcactttcta
    21301 caacagataa tatgaactat attttggata ttactacctc gccttggtat gactgttctt
    21361 atgaagacat ttttgcgttt actgtaaacc aaattacaaa aagaaaaaaa gatttttcgc
    21421 tttttgtaaa acttaaaaaa tatacggtta aagcagaaat atttgaagaa tttttgcgcc
    21481 aaaaaggttt taattgtgtt caaactcatt taattttggt aaaagacttt tataaattag
    21541 ttaaagagcc tgaatctgct cttaagatag taatgtttaa cgaagcaagc gaaaaacctg
    21601 tttttaagat tgagggtttt tgagttaaaa taaattcatg aaaaaatttg aaaaatacat
    21661 gaaaaaatgc ttgaagcttg cacaaaaagg cgacggaaaa acttctccaa acccacttgt
    21721 gggctgtgtt gttttggata aaaacggaaa agaaatttca agcggctatc ataaaaaata
    21781 cggctcactt catgccgaag ccgatgccct gtcaaagctg aaagataatc agataaaagg
    21841 ctctaccttg atagttaatc ttgaaccatg tgttcatttt gggaaaaccc cgccttgtgc
    21901 tgatttgatt attgaaaagg gtgttaaaac gcttgttgtt gcaatgaaag accctaatcc
    21961 tgttgtttca ggcaagggaa tcgaaaaatg taaaaaagcc ggtattaatg ttcttgaagg
    22021 gattttggaa gaagaagcaa ggcagttaaa tgaagttttt ataaaaaaca tggaaaaaag
    22081 ggcgattttt gtcgcgacaa aaatcgcctc aactcttgac ggcaaaatcg ccgcgcaaaa
    22141 cggtaaatcc aagtggatta caggcgaata cgcccgtgaa gaagttcaaa aaataagaaa
    22201 ccgctatgat gcaattctga caacgagcgc aactgtgatt aaagataacc cttcgcttac
    22261 atgcagaata aaaaacggca aaaatcctat caggattgtg cttgataagg atttaaaaac
    22321 agattttaag tcaaaaattt atgaaaataa ttgtgaaaaa atttatgttg cagttgataa
    22381 aaacacagat aaaacgaggt tatctaccgt tcctgctcat gtcgaaatca taaaatgccc
    22441 ccttaaagct tcaaaaatcg accttaattt tcttttcaaa aaactttttg aaaaagaaat
    22501 aagaagtata ttgattgagg caggcggaaa ccttaacggg gagttgattt ttcaagattt
    22561 agcggataaa atttaccttt ttatggcacc taaaattatg aatgataaaa acgcaataaa
    22621 ttcgttttat ggtgaaaaaa cctttaaaat agataattta gcacggttta aaatttctaa
    22681 aattgataaa attgcagagg acgttctgtt tattttaaac aaataaaaaa aacgggacaa
    22741 atgtcccgtg agtcttgtta tgctgtgttt atatgttaag ttaggagttt attgtatcta
    22801 agccaaaact ctgaaaattt catcttgtat gtcaaagtct tcgtatgaag ggggtgtctt
    22861 aacaaagtct ttaacaaatt tgcttatttt gttaattatt tttgaaaata tgttcttttt
    22921 aggtattacg cttttgaatt gaacgtctgt caattcatag gttgaagatt ttatgtcttt
    22981 atttatttca ttcttattgg acaagaatga aatgtttttg tagctattta attttattgg
    23041 gctaacggta atcattgttt tctccttgag aattaccatt ctctttttat taagtgtttt
    23101 tctaacactt catcttacat tttcattatg tatcataaag aaaaatttgt caaccccctt
    23161 ttgttaataa aatgttaaga ttgtaaaaac cagtattaat gggctatttg ccataaagtg
    23221 ccaaatgtac acttaatata gtggtcttgg ggctatgttt tcatatcgaa attacctgat
    23281 aagctaggtt tttattttta aaatataaga cgactgatta attgaaaaat tatcgtttat
    23341 tgtttttaat gagtttaaat ctttttcata cttccagcta ttcttaattc caacagggcc
    23401 gtaaggccct tttctttttg tggaaatggg tagtaaatgg tgaagggtga agagtgaagg
    23461 gttataaaaa atccgtcatt gcgaggcgta gccgaagcaa tccaaaaatt ggtgcgcatt
    23521 gcgcacccta ctttcttata aactcatcaa cttataatct tataaactaa actattcaaa
    23581 ttatcgactg ttcgactatt taactaaact tcagccgatt ttcttcattg taggaaacgc
    23641 tataacctgt cttatgcttt gagaatccgt taaaagcatt gtgagacggt caattcccat
    23701 acccatgcca cctgccggcg gcatgccgta ttcaagtgcg ttaataaaat cttcatcaat
    23761 atcgcatgct tcttcatcgc ccatttcttt ggctctggct tgagcctcaa agcggcttct
    23821 ttggtcaatc gggtcggtaa gttcagaaaa agcgtttgct atttcccagc cgtttattct
    23881 ggactcaaac cgttctgtca gcctgtcgtt gtccctgtgc acttttgcaa gcggtgatat
    23941 atctctgggg taatcgatta tgtgaattgg ttgaattagg tgaggctcaa ctttttcttc
    24001 aaaaattttg tctaaaatct gtccccagtt atcggtttct tctacttcta tgcccatatc
    24061 ttttgctttt tgggcggctt cttgggcggt gtacatcgtg ttaaaatcaa ttcctgtata
    24121 ttctttaatc gcgccgagca tggtttttct gtcccatggc ggggtcaagt cgatttcttt
    24181 tccgtcaaaa tttattttca tagtgcccaa aaggtcttga gcaactgaac ttaccatatt
    24241 ttctgtaagt gccatcattt cgttgtaatc aacataagcc tgataaagtt caatcattgt
    24301 aaattcaggg ttatgccttg tatcgcagcc ttcgtttctg aaacttctgt taatttcaaa
    24361 aatcttttcg ctcaaaccgc ccaccaaaag ccttttcagg taaagctcag gtgcaattct
    24421 caaatacatt tccatatcaa gggcattatg atgtgtaata aagggccttg cgtttgctcc
    24481 gcctgcctga gggtgaagca taggggtttc aacttcaaga aagccttgtt gagtgaggta
    24541 ttctctgatt ttttgaatta taaggcttct ttttctcaag acttctcttg attcttcatt
    24601 cactatcaaa tcaacgtacc tttggcggta gcgtgtttct atgtcagtga gtccgtgaaa
    24661 tttttcgggt aacggtaaaa gtgctttcga caaaagctta taattttttg cttttattga
    24721 aagttcgcct ctgggggttc ttctcattga gccgtaaaac cctacaatgt cgcctatatc
    24781 tataagttta agcgttttta tcgcttcttc atccatgttt tctttatggc agaaaatctg
    24841 aattttgcct gtggcatcca ttaaatcaat aaacatgcct gtatttcgca ttgccatgac
    24901 tctgccggca accgaataaa agtcttgggt ttcttctccg acgggcaaat ctttatactt
    24961 ttcctgcaaa tcgtttgcat ttgcatcttt gctgtaagaa taaggataag gattaattcc
    25021 tttatcggca aggtcagcta atttttgaac tcttgtttgt cttatcgagt ctttttgtga
    25081 tgaatgttct tgtatttctt ctgtcatatt tccctctttt tttaactttt ctaaaattat
    25141 tttagcatga aaataagcaa tagtgaaagg gcgagcagtg agcagtgaga ggtgagaggt
    25201 gagaggtgag agcgggattt tcaaagaaaa tcccgctctc acctctttta tgtcattgcg
    25261 aggggtttac ccagaagcaa tctaaaactc ggttcgcaaa acgtatttta tagcggctat
    25321 ttaccgttca ctgctcaccg ttcacttttt taatttatcc ctgcaggtgt cgccatcgga
    25381 atcggtgcaa gctgatttgg tttagaggct tttggtgcag aaatttgttt tggcgttgca
    25441 ggggctgaag gcttttttac aggctgttca acatcggaag gaagaatctc attgttttcc
    25501 aatgttgttg aatctgttgc attttcatct gttatttctt cttcgccttc ggctttttct
    25561 tctttttgtg ctgcagaata atttttaagt tcaacttttg ggtaatcaaa atcagcattt
    25621 ccgtaagggg cggttgctac tttcattaca tctctccaaa taagagcagg aacagtacca
    25681 ccggtgagat ttcctatttt tgtgttgtca tcgtttccga cccaaacgcc cgtaacaaca
    25741 tcgggcgtgt agccaataaa gcatgcgtct ttgtagtcgt ctgttgttcc tgtttttcct
    25801 gcggctggtt tgcctatatt tgcagcaacc cctgttccgc ttttcattac ggtttttagc
    25861 attgccgtca taattgctgc tgtatttgtg tctattactt ttgaaatttt tgccttaggt
    25921 gcctgataaa tcacctgccc gcgcgaactt tccactcttt ctattccgta gggttcaact
    25981 ctgtatccgc cgtttgcaaa cgctccgtat gcccttgtta tttcaaaaag ttttacgccg
    26041 tttgagccca aggctattgt ataatcgtat tcaagcgggg ttgaaattcc taaaagcctt
    26101 gccatttgaa tgacggagcg aattcctgtt gactggatta ctcttgcggc gcagacgttt
    26161 gaagatatca ttagggcttt gtatgcagga attttacctc tgtatttgtt gtgataattt
    26221 ctcggtgccc agtcgccggc tttaaatggc gcatcttcaa ttaagtcatt cgggtcaagt
    26281 cctttttcaa ctgcagcagc ataaataaaa ggtttaaaag cagagccggg tggtcttacc
    26341 gcttgagtta ccctgtcgta ttggcttttt gaatagtctt ttcctccggc ataagctaat
    26401 attcttccgt cgataggact gaatgaaaat actgccgctt gttgtttttc gcctcttagt
    26461 ccccaaccgt ttaagtttct tatgattgct tcgttagttt gttgttgagt tttgtaatct
    26521 attgttgtta taactttgta gccgccttgt gaaatttcta cttcatcgaa cccgagtttc
    26581 tcaagttctt tcattacgta gtcgcaaaaa taaggtgctt tgttaagggt ataaatttca
    26641 ggaagcgggt ttagttttaa tttttcttta actgcttttt gatattcttt ttttgttata
    26701 aaacgcattt tatacattct tttaagaact tgatttctgc gttcgattgc taaatcttca
    26761 ttattaaaag gcgaataaac cgaaggtgct tgcggcaagc cggcaagaag ggcactttct
    26821 gcaagcgtca aatcttttaa atgtttgttg aaataaattt gggaagcccc ttccactccg
    26881 taagctccag agcccaaata gacattattt aaatacattt caagaatttt acttttgggg
    26941 atagtttttt caattcttgc tgcgattatc aattctttta tctttcttgt aaaagttttt
    27001 tcgtttgaca agaataaaat ccttgcaagc tgttgtgtta ttgtgcttgc accttgaacg
    27061 acatgtcctg ccaaaacgtt tgcaaatgtc gagcgtacaa gccctgtaat atcatagcct
    27121 ctgtgcttat aaaaattctt gtcttctgtt gctatgattg cgtttttaag gttgtcggga
    27181 atatctttta aatcaacttt tgaaaaagta aatgcggtaa aagtttttat tatttcatcg
    27241 tctgaagaat aaaattttgt tacaatgttc ggtttaaatt catctaagtt gcgaatgggc
    27301 ggaagcgaaa gcaagtaaac atgaaatgcc acaattcccg ctaaaagtgc tgaaaccaca
    27361 ataagtacta catttgcaat atcgggattg atatttgtgg attttgcttt tttaattctt
    27421 ttgggtactt gaaattcgga cattttaaaa ctctcaacta ttacatcatg atattattat
    27481 agaataataa ttttacaggt ctatattgta aacatgttta acttattttt taacattata
    27541 aatgaaataa aatcatattt cgtagttgaa aaaaccgagt atgaaataat aggcgagtgc
    27601 aaaaaatgcg gtaaatgctg caattacatg tacagttttg atacatatac agaaaaagaa
    27661 ttcaaattca tgcagttttt gtatcctgct tacaaaaggt tttacataaa aggaaaagac
    27721 gaggaaggaa actttatttt tgcctgtaaa tacgttacaa aagaaggttt atgttcggtt
    27781 tatgataaaa ggcttaagat gtgcaaaatt tatcctttca ggaaaatcaa ataccctgca
    27841 aaacttcacg aaggctgcgg atataaggtt ttgaaaaaag attttgagga ttatttaaat
    27901 aagtaatttt cttgcatgtt tggtttgtat attataaaat cgaaggcgga tagctaagaa
    27961 gaggttttta tgaagaagat tttatttaag ttagcgttgg gactttttgc attgtcttat
    28021 atgattttgc cctcatgcgc aaaggttttt cctgatgttg ccgataacca ttgggcatca
    28081 agccaaatta aagaattatc gcaggcagga gttattgtcg gatatcccga cggaactttt
    28141 aagcccgatg caaatgttac aagagcagaa tttgcatgta tggcaataag agctcttaaa
    28201 caggaaaaca caaaggttat tcagcccgtt gattttacgg atataaatga agattattgg
    28261 gcttacgaca tgattcaaag ggcactttat tttgaattaa tctcatgccc gccttcggga
    28321 aaacttttca ggcccgatga ttctgtttcg cacgctgaag ctttgaccat gtcggttaat
    28381 gcgcttacaa cagaaacaat aagtgcgcaa aaagcaagcg aggtcttaaa agtctatacc
    28441 gaccttaatc aaatccccga ctggtttttg ataactgcag gtaaagctga aattttgtca
    28501 atgaatgtta ttattcccaa tgtcccgcgc acaatgaatg ccgataaacc cgcaacacgt
    28561 gccgaaatta cggttttgtt aagcaacatg atggcgcagg caaagcttaa tcccaataaa
    28621 aaacttgccg aagcaatgcg caaaaaaaca ggtgacggtt ttgttattga aagtgccttt
    28681 gttcagggaa cggtaggtac aattccggca ggttcgataa ttcctattca gttgaccgct
    28741 ccaatcagtt cgcaaacttc taaattggga gaaatttacc ttgcaaaagc ccctgaaaat
    28801 tatattacaa gagataaata tattcttatt tacaaaaatt ctgacctgaa aggtaagtta
    28861 atagatgtca gagaaggtaa actttttgtc agaaacggca tgttaatact tgacaacgac
    28921 actcttgtga cgcctaatga ccaaacaatt aactttcatg gtgttggtga agtttgtgtg
    28981 aagaaaaatt ggtttgttgc ctttttgaga aaagtgttta aaggtgaaaa gcttaaggta
    29041 gcacctaaag gcactgttta tgtcaagctc cttcagcccg ttaaagtcga tctttgtaac
    29101 ggctggatat tggaataatt gatttatgtt aaatagacaa acggcggatt tttcgaagaa
    29161 aaatccgccg tttgtttgtg ttgtgattgc caaatcatgc tataattatt ttcatgaaaa
    29221 agattttatt aagtttagtg tttatatttg ttttttgccc aagggtattt tcgggcgatt
    29281 attgtgcggt cggggttgat cattttaaaa aaggcaatta cgaaaaggca atgtataacc
    29341 ttgaaaaagc cgttaaagct tcaccacaga atgtaaatgc caggtattat ctggctcagt
    29401 gctatatttt gcaaaaacgt aattctgatg cgcttaatca gtacaataga ataattttac
    29461 tggcaccttc gtcagatgct gcaaggcttt ctgttgtggg aatttcttta atcgagcagt
    29521 cttacaacgg caaatcggtt gttggagttg gtttattatc ggatgagttg gcaaagtata
    29581 aggataatta ttttgattat gtcgtaacag gtgacaatgc ccttttcagg tgggcaagtt
    29641 ttcccgtgag ggtttatatt cagccgtctg ctcagcaggg aattgttaaa aaagcattcg
    29701 atgaatggca aaacaaaaca ggaaatttgg tgaaatttca atttgtaaat tcagcgcaaa
    29761 atgcccaaat taatgttgat tttcttcaac agttggagtc aacgtccaca aaagaagctt
    29821 atgtggcggg gtattcaaag ccttattata aggacggaaa aatagttaag tcggatgttc
    29881 acattttggc gacagagccc gatacaggtc agcctgttaa caatgatttt ctttattttt
    29941 ccgcgcttca cgaaatcggg catgctttgg gatttagagg gcacagcccg aatgaaaacg
    30001 atgttatgtt cgctcaggca aaaactccga agttaagcct ttcgcaaaga gatattaaca
    30061 ctatgaatgt tttttacagg attaatgaaa agactttaac ggcaaaaggg ggcgggcagt
    30121 cggatgtaaa gcttcagcaa gcggttgatt attcaaaaca gtttcctgaa aaatcggtaa
    30181 gctggacggg gctgggcgat atttacatgg gtaaaaaaat gtatcctgat gcgataaaaa
    30241 attataaaaa agcggtagcg attgagccgc aaaaagcgga tttatatgca cttttgggca
    30301 atgcttacag tgcttcgggg gattcaagaa acgcttttgt gaacttaaga caggcttgcg
    30361 atttggataa aaataacggg gattatgttt ataattttgc agttttttgc gccaaaaacg
    30421 gacaaaaaca aattggcgag cagtatgtta aatcttattt gactgccaat cctcaagcac
    30481 tttctgacag taaaatgcag tcacttttgc aatcatataa ataattatta agtattgtaa
    30541 acccttctaa tgcagtattt aaaggtattc caaaaaaaag gtgtaaaatt accctgttgg
    30601 actagcaaaa aatttcttga tgagttttaa ttaagagtgg acttcacttt attcaaaaaa
    30661 ggtcgtttat cctgcgaata aatgaaagaa acgagttttg tacagaatgt gtagaaatgg
    30721 agtaacttat gcgagtaaga aatgattttt cttatgacgt acagaagaga cagggaaaga
    30781 gagacatagg tttttctaaa ttttcgtttg gggcggatgc tccggcacct gctcagcctg
    30841 cgcaaaaagt tgaagaacct aaagaaaaag gatcttttat caaaaaagca accaactttt
    30901 taggtgttgc ggcttggttg tttatggttg gttatagtat caaaaaattg agacttttag
    30961 aaccttcata tcatgaaaat gttgcaaaaa gtgcaaaaaa ttattcaaaa aggactttaa
    31021 gctacattga aaaattagct gatcctgaag cggctaatat atcaaaagtg ttagaggaaa
    31081 aaggatttaa aggtccttca aaatttgtaa ggaaagcttt ttacggaatc ggtgattggt
    31141 ttcagaattg gaaaatgaac atgggcagtg agcttgttaa taacttgact tatgcttttg
    31201 gaacgttggt tgtaatgcct ttagttgttt tatttagccc atttggtaag aaaaattcat
    31261 ctaaagaaga caaggccttt gctgttttac gacaacccct ttctgttgct gcaactttag
    31321 gtatgcaatt tacttttgac aaattgatag ataaatatgt tcctgaagta ttaaaacaaa
    31381 acaaatttga agaacctgca attgttggag aaaacggcaa aattgtttgg aaaaatcaaa
    31441 ataatgaatt tttagcggat aattttaata aaataattta taataaagac gaggctaaag
    31501 aagcatttaa agaatatgct aaaaatgttc tttcggaaga cgaaataaaa gaactattta
    31561 cggatagatc ttttgaagaa ggatcaacat cggaattaaa aacaaggtta aaagatattt
    31621 taaacaaaaa gtatgcggaa gcgggtctga agcttgataa aatagaaaat gttgaggtta
    31681 atgatataaa tagaaaagca agttcatttt ctgaatttaa aaagatagaa aaaatcgcag
    31741 ccgaaaacat tgaagacttg ttagttaaat ttaagaaatt cgcttctgtt ttagataata
    31801 ataaaatggc aatacaaaaa tgtaagacag gtgtgaatgt agttgctgct tctataatag
    31861 gatgtacttt cttgaatgtt atatacggta aatttatgaa ggctcttaaa aaacctcaag
    31921 aacaacaggc aaaggaggtt aaataatgac tattgatatc tctaatgctt taaataattt
    31981 gaatatttca aaaatagagg ttaaacttga tgaaactttt gattatgttt taaagcaaaa
    32041 agaagctaaa aaacaattta aaattttatc aaaaatagct tctaataaag atatttttga
    32101 taaagaaaat attgatgctc ttaatattgt taaaacccat ttggaaactt taaaagcgaa
    32161 ttttgagacc atagaaagtg tcacaaaaaa agtagctggt gaaataaaaa ttccaaaagt
    32221 atttaatcat gtagttaaac tgcttggaaa ggttaaaatt gataaaacag gtaacaaagc
    32281 cttagatact gcttctgcgc ttacacatgt tgttttatgg ggcaatgtag gaaaagaagt
    32341 tgtaggtacc gctatgtaca ccgtacaagc tttaactaat gaagatttgc ctaaagataa
    32401 acgtaaattt gtaggcatgt acgatttggc tgttggtgtt gtatcgactt gtttttcttt
    32461 catatttggt gtggggctag aaaatacaat taaaaacggc tataaaaaac ttttaaaacc
    32521 gttggccgaa tccgaaaatc ttgcactaaa atcaagatca ggtgcagcaa tagtcggttt
    32581 agcagcattt tcatcattta tactacagac tattgttggt aaaagaatgg tggctccagc
    32641 ggtagctacc cctgttgcgg gtaaattgaa gaaaaaattg gaagaaagag aaaatggtaa
    32701 aaagccggtt gatttaacag caactgactt gaacataggt ttcccaaaag tcggggataa
    32761 ttcccaagga gcaggcacta ccaatctgct tgataaagca agggctttaa gcgttaaagc
    32821 ttagcttaat taaataaata aaaaagcggc gaagttttta aacttcgccg cttttttata
    32881 aaaatttaat atctctattg tccggctttc gcccctaccg ccggctgtcc cgcagccggt
    32941 agggggctta gcacttctta ggtcggattt gaaaaattgc gaaaaatcgt catttttcga
    33001 atttttctgc atcctctttg tcaaaaaaac ttgtttgtag taccatgaaa tagtattgat
    33061 aattaagaag gagaacaaaa tgtctggtaa aacaataaca gttgacggaa actacgctgc
    33121 agcgcatgtc gcgtatgctt taagcgaagt atcggctata tatccaatca cgccttcatc
    33181 tacaatgggc gaatgggttg atgaatgggc ttcacagcac aagaaaaata tttggggaac
    33241 agaagtaaaa gttgcggaaa tgcaatcaga agcaggtgca gcaggtgccg ttcatggctc
    33301 tttagcagca ggctgtttga cctcaactta tacagcatca caaggtttgt tgttaatgat
    33361 tcccgttatg cacaaagctg caggggaatt gcttccacac gtaatgcacg ttacggctcg
    33421 ttctttagcg gctcaatcgt tgtcaatatt tggcgatcac tcagatgtta tgggctgtcg
    33481 taatacaggt tatgcgatgc tggctgctaa ttcagttcag gaagaaatgg atttggcact
    33541 tgttgcacac ttagcaacat acaagtctaa agttcctttc cttcaattct tcgacggatt
    33601 cagaacttct cacgaagtac ataaaatcga agaaatctct tacgaagaca ttgcaaaatt
    33661 agttgaacct caatatattg aagaatttaa aaaccgcgca ttaaaacccg aagctccgat
    33721 ttgtaaagtc ggtgcgcaaa accctgatgt ttatttccag gggcgagaaa cagtcaataa
    33781 attttatgat gcagtgcctg atattgttca ggaatatatg gataaagtcg caaaagttac
    33841 aggcagacag tatcacttgt ttgattatgt aggcgcgtct gatgcgacag atgtaattgt
    33901 tgcaataggt tctgcttgcg aaacaatcga agaaacaatc gagtacttga attctaaacg
    33961 cggtaaaaaa tatggcttga ttaaagtcag attatacaga ccgtttgatg taaaaagatt
    34021 taatgaagca cttccaaaat ccacaaaacg ggttattgct ctcgacagaa caaaagaacc
    34081 cggcgcaatc ggcgaacctt tatatatgga tgttgtcgct gctataggat gtcaaaaaga
    34141 catcagagta atcggcggaa gatacggttt atcttctaaa gaattcactc cgtcaatggc
    34201 tttggctgtt tacaagcatt ccgaaaacaa cggattccat ggctttacgg tgggtattga
    34261 agatgacata accaatcttt cattaaaaat tgatgaacag attgttacag agcccgaagg
    34321 cactacaaac tgtatgttct ggggcttagg ctctgacggt acggtcggtg ctaataaaag
    34381 ttctattaaa attatcgggg attacacaaa caaagatgct caggcatatt ttgcttatga
    34441 ctccaaaaaa tcttatggtg taacggtgtc tcacttaaga ttcggggaca aacaaattaa
    34501 atcaacctat ttaataacag cacccgattt tgtatcttgc tctgctcatt catatatagg
    34561 cagatacgat ttgttgaaag gtattagtga aggcggaact ttcttgctta acagtcctta
    34621 ttcaaaagat gaggtatttt gccacttaac aaaagatatg caggaaacta ttattaacaa
    34681 aaaaataaaa ttctacaatg ttaatgcaga agaattgatt aaacagcagc ccggacttcg
    34741 cggaaaaggt gcaaacactg ttatgatggt tgctttcttt aaagtttcgg ggattattcc
    34801 ttttgaacag gcatacgaag gcatgaaaaa tatgactgtt aaaaccttta agaaaaaagg
    34861 cgacgatgtt gtaaatatga acttagcttt aatagatgca gcgattaacg catgtgaaga
    34921 aatacctgta ccttcttcgt tggatgtaaa ttgtgtttct gctgcaaaat tgcttcccga
    34981 tgatgcgagt gattttgcaa agaaaatcat tgaaccttcg atgaaacaaa aaggagatga
    35041 tattcctgtt tctgcgatgt cggttgacgg ggttattccg acatctactt caaaacttga
    35101 aaaacgctgc atagctccga gagttcctca ctggaattcc gaaacctgca ttcaatgcgg
    35161 aatttgtgct tcagcttgtc ctcatgcagt tataagaaca aaattaatta tgcctgaaga
    35221 cttaaaaaat gcacctgcaa catttaatac aatagatgca aaacccggtg acggtgagaa
    35281 attcaaagtt caggtttact gtgaagactg taccggctgc ggagtttgtg ttgatcaatg
    35341 tcctattaat attaataaac ctgaaacacc ggccttatcc tggtcaacaa tcgaggctga
    35401 aaaagaagca ggcgaaaaag aaaatgaaat tttctttgag gcacttcctg ataatgttat
    35461 gggcaaaaac acaaccaaaa caattaaagg cgcaatgctc agaaaaccgt tattcgagtt
    35521 ttcaggagct tgtgcgggtt gcggtgaaac tccttatgtt aaacttgtat cgcaattatt
    35581 cggcgaaaac atgattgttg caaacgcaac gggatgttca tcgatttatt cgggaacttt
    35641 ccctaccatt ccttatacaa caagcaaaga aggaagaggt cctgcttggg caaattcctt
    35701 atttgaggat aatgccgaat tcggttttgg gatgagattg gcggttgacc aaaacagaga
    35761 aacattaaaa ttatatgctg aaaaacttct tgactgtgaa aaaacgcccg tggagcttaa
    35821 agagtctatt aaagcatctt tggcactttg ggatgactca aaaacagacg aagcaattaa
    35881 agctcaggac aaagtaaaag aacttcttgc taaaaccgac tgtgattgtg tagaattggc
    35941 aaaagtaaaa gaacttcaag actatttcac ggataaatca gtttggatta taggcggaga
    36001 cggttgggca aatgatatcg gatacggcgg tattgaccat gtattggcgc aaggcaaaga
    36061 cgtaaacatc cttgttcttg acacagaggt ttactctaat acaggaggac aagcctctaa
    36121 agctactcct acagctgctg tagcaaaatt tgccaccggt ggcaaaccta cttacaagaa
    36181 aaacatggga cttatgagca tgtcttacgg tcatgtttat gtagcatcag ttgcactcgg
    36241 cgcggacaga ggtcaaactc ttaaagctct acaggaagcc gaagcttaca atggaccttc
    36301 cataatattt gcgtatgccc cttgtatagc gcatggtatt gatatgagca aaacccaaac
    36361 agaagaaaaa cgcgctgttg aagccggtta cttcccgtta tacagatata accctgctaa
    36421 tgaacaaccg ttcagttggg atgcaaaaga gactaaagga agctatcagg actttatcag
    36481 aagcgaagga cgttataaac agcttatgaa aacaaatccc gaagcggcag aggctcttta
    36541 caaacaggca gaagatgatg cttcaaaaag aatggctgtt tacaaagcag tcggtgaatt
    36601 gatgaagtaa ttttaattaa attaagggcg ggtattttta tccgcctttt taattataaa
    36661 ggtgtaaagt gggttggggt ttccacccca acaacatgag gttaaaaatt atgtttaaca
    36721 aagaaaaacc cgttgaagtc attattgaca aagaaaaatg taccaagtgc gggatttgta
    36781 tcggttattg tcatgaattt ttaggcaaag accaggatgg ttatccaaaa gctatgcctg
    36841 ttgaaaacac tcttgccggt tgtattcaat gcggacattg tatgatggca tgtccgaatt
    36901 ctgctataga aattaaaggt gaagacattt caaaaagcca tttgcgagaa tttggcaatg
    36961 ccgcaaattt tgaagaattg aataatctgt ttttaaaaag aagaagcata agaaaattta
    37021 aaaaagatga agtttcaaaa gaagttttag ataaaattat tcaagccgca gcgactgcag
    37081 ctgtaagcat tccgccttcg gaattaaagg ttttggtgat aagcggcagt aaaaaagttc
    37141 aggaacttgc cgaagaaatt gttcaaggca tgaaaaaatt tccaaaaatg ttcaacccaa
    37201 tatctttgaa tttgatgaaa atttttgcag gtgaaacaaa ttacagaatg tttaaagaat
    37261 ttattattcc gctttgtaaa attaccgttg aagcacgaga tagaggcgaa gattaccttt
    37321 tttataatgc gcctgctgta attttatttt acagcaccga attaagcgac aaagaagatt
    37381 ctcttatcgc tgcgacacag gcaacacttg ctgccgaagc actcgggctt ggaacttgct
    37441 ggataggttc attaggtgcg gtttttcagc ataacgaaaa gctgcgtaaa aaatacggca
    37501 ttttaaaaaa tgagaaaatc ggctcaggct ttgttgtcgg ctatcccgat accaagtttt
    37561 acagggcatt tcagcgaaac tttaagaatg taacatttat ttcatagttt tgcgcaatat
    37621 tttattagaa aaatacttaa ttaaagtatg tagaagttat ttttgtggag gaattttatg
    37681 tcagttaatt tacctattat agaaaaagta gcaggaaaag tttttgtttg tagcaaagga
    37741 gttgaccctg ttaaaatcga atgtgcaagt gaaactattg ctacgcaaat tgcgcatcag
    37801 gtgaaaatgt tggcaataac attgggtaaa aagttagata aaacagttta aaaataaatg
    37861 aaaggaaggt cattatgtca gaatcaattg cgagtattgt aaaaaaagac gggcaaattt
    37921 ttgttaatgt taagcctgca ggggccaaag aaggtaaatt aggcgaaggc ggagttttag
    37981 ttaagtgtaa agatgaagaa gaagcaaaag caatggtaaa agaaattgac gctgcggcaa
    38041 ttaaagaagc aaccttaaaa gaaggcgaag gtaagaaaat agacaaagca gcataactta
    38101 cttataaatt gtgaaaaagg cgggtaagga taaaatatcc ttacccgcct tttttctaaa
    38161 aaatatcgcc ataaaagtta ataaattttt tatttgtaag tttttatttt ataattatga
    38221 tataggggtt ttggcgtaaa gtttgagcca aagaagagag gggaattaat ggaaattgaa
    38281 ataataaaac aaacagatcc tgaagtcgcc cagtttgttg aagctgaatt aaaaaggcag
    38341 caggcaacaa ttgagcttat agcaagcgaa aattttacat caaaagccgt aatggctgct
    38401 tgcggaactg ttttaaccaa taaatacgca gaaggaaaac cgcacaaaag attttataac
    38461 ggatgtgaaa atgttgatgc gatagaagag ttggcgcagg aaagagcaaa aaaactcttt
    38521 aatgtagcac atgccaacgt tcagccccat agcggagcac aggcaaatat ggcggtattt
    38581 ttgtacgcac tcaaagccgg tgataaagtt atgggaatgg atttgtcaaa cggcggtcac
    38641 ttaactcacg gctcgcctgt taatttttca gggctttact ttgatatcgt aagctacgga
    38701 gtagatgaaa atggctgtat tgattacaac gaagtcgaaa aacttgccca aaaagaaaaa
    38761 cccaaaatga taatttgtgg cgcaagcaat tattcaaaaa ttattgactt taaaagattc
    38821 agagaaatag cagatggcat aggcgcgtat ttaatggcag atatcgcaca cattgcaggg
    38881 cttgtggcag gcggagtcca tccttcgccc tcggtatatg cccatttcat aacaaccaca
    38941 acgcacaaga ccttaagagg accaagaggc gggataatta tgtgcgggga agaacatgcc
    39001 gcaggaattg ataaagccgt atttccgggt attcaaggcg gtcctttgga gcacattatc
    39061 gcaggaaaag ccattgcgct tttggaagct tcaaagcctg aatttaagct ttacgcacag
    39121 caaattatca aaaatgcaca agcccttgca aacgggctta tcggcgaaac catggatatt
    39181 gtcggcggat gtactgaaaa ccatttaatg acgcttgatt taagaaaatt aaacaaaaca
    39241 ggcaaagaca tggcaaatgt ccttgagcgt gttggtataa ccgcaaacaa aaataccgtt
    39301 ccaaacgacc cgcaaagtcc ttttgtgaca agcggaataa gactcggaac acctgcagtt
    39361 acaacaagag ggtttaggga agatgatatg gatgaagttg caaaaatcat tgcatctgca
    39421 gtattttcgt cggataatga agtcggctta aataatttgc gcgaacgttc gctgaaatta
    39481 tgccaaaaat atccgttgta ttaaaggagt aaaaccttga tcatcaaatt atcagaagca
    39541 catataatcg gcgcaataat ttcctacttg ttaggagtat tcatcgtgcc tttggtaatt
    39601 tcatattcag aaaaacaagg tttgattgat gaaccgaacg aaagaaaaat tcacagaaag
    39661 cctatttcaa gacttggcgg gattgcaatt tggctaagca ctatgctcac ttttctcttc
    39721 cttgtgtttt tgagttatta cccttacgga agtttggttt cggggatact actgggcagt
    39781 tcactgatgt ttttcttggg gcttattgat gatatttata atctggatgc aaaatttaaa
    39841 ctttttatac aaattgcgat tgtaactttg gtatattttt tgggcgtaaa aatagacacg
    39901 attataaacc ccttcggcgg ggttatacat cttgggcatt tttcttacat aataaccgtt
    39961 ttgtggatag tgggaataag caatgcggta aacttcattg acggagttga cggacttgca
    40021 ggctctgtta taacagttaa ttctataaca cttgcaataa tcgctgttgc aatggttcca
    40081 ccaagtccta taagtgcgtt gataggcttt atacttgcag gttcaatgct tgcgttttta
    40141 acatataatt ttaatccggc aaaaatattt atgggagatt ccggtgcttt atttgcaggc
    40201 tttttgcttg cggcactttc gattacagga gttatgaagg cagcagccct tgccattatt
    40261 ttaccttttg tggttttagc ggtgccgatt gctgatatta cttattcaag tttgagaaga
    40321 atttccaaag gaaaatcgcc ttttgttgca gatgccgaac acatccacca caaactttta
    40381 aaagcaggat tcacccaaaa gaaaaccgtt ttgatactga cttctgttgc tgtttttgca
    40441 ggtgctgttg ctgcgttttt tgcaggctca atcaaacatt atatcgccta tattcttgcg
    40501 cttatagtaa ttatggttgt gttgaattta ataaaaattt ttaaaaaaag tgcttaatga
    40561 gggcacggaa taattccgaa atatacaatt tcgagaattt tccgcaacct caatttttaa
    40621 acatttcttt tctttgtttt tttatttcac aaatttgttc aaaataatct ttgttcagta
    40681 gatctcccac agatgaataa agctttaaaa gagcattttg gatgttatca gaattaagat
    40741 taatcatatc tttagccatt tcttcgtttg aaattgcaag acgtgtcata tccctgaacc
    40801 cgcttgaggc gatttttaag gcaagttcat tgcagcaagc cgccttaaaa atcgcctgcg
    40861 aaatcaacat cggcatatga gaaataagcg caactgcttc atcatgagcc tccggtgtcg
    40921 tgaaaacagg attcgcaccg agcgatttta tgatattttc caatttttta atatcctctt
    40981 ttaagttttt atccaaagga gttaaaaccc agtttgcttc tttaaacaga ccttcaatgg
    41041 aattatcaaa tccgctaaat tcgcttcctg ccataggatg ccccggaata aatttgaatt
    41101 tatatttctg tgggcggttt ttgctttgca aaaaccgctt caaactgcaa acatctgtta
    41161 cgattgtatt ttcatttaaa atattttcca attcatcaag agttgaaatg gttttgttca
    41221 tcggagtaca gacaaaaaca atatcgcagt cattaagaga ttgaagcgaa tctgtggcaa
    41281 atgaatattt ttttgctttt tcgattgtac ctgcactttt tgaaaccccg ataacatcaa
    41341 agcccaaagc gcaaagttct ttaaatatgc ttcctccgat aagccctaat ccgacaacgc
    41401 ctatttttaa attcttatcc ataaaaaact cctaaattaa atcttccagc aatttttcaa
    41461 aatccgggaa ggatatatta acccattcaa aatcattgat taaaatcggc tcgtaacaga
    41521 ttaaacccgc cacataaagc atcattgcaa gtctgtggtc gtggtaactt tcgacttcta
    41581 ctccgccttt aagttttgat ttgcctttaa tcctaaatcc gtctgcagtt tcttcaatct
    41641 cagaacccag tttttttagt tccgaaacaa ccgcgcttat tctgtctgat tctttatttc
    41701 ttaaatcctg agcatcttta atcacagttt cgccctgtgc ttgagttgca agaagagcga
    41761 ttatgggaag ctcatcaata agttttggta taagttctcc gctaatctcg caagccttta
    41821 attctgaaaa tctgactctc aaatcaccga cttgctcatt tgaaataagt tttttatcta
    41881 aaatttcaat atctgcaccc atttcttgca aaacttctaa aatccctgtt cttgtggggt
    41941 ttaacccgac atttttaatt agaatatcgg aatttttaac tatcaatgct gcaacaataa
    42001 aaaatgccgc agaggaaata tcgccgggta cagttattgt ttttggctca aattgtgatt
    42061 tttcaatagt aatagtattt ccttgaacag aaatttttgc gcccaaatat tcaagcattc
    42121 tttccgtgtg gtttctggaa ataaaaggtt cggtgaaagt tgtttttccc tgtgcaaaaa
    42181 gtccggctaa aagcacgcag gatttgacct gtgccgaaga aatcggggaa tggtaatcaa
    42241 tcccgtttaa ttttgaggct aaaattttaa gaggagccgt aaagcagacc ttagtaaaat
    42301 tcgattcttg gatagatcct gaaacaagtt caggatgacg ttgaggtgat ttacttgcaa
    42361 taatttttgc tcccataagt tccagcggct ctataatcct tttcataggg cgttttgaca
    42421 ggctttcatc gcctgttaaa acacaatcaa aattttgtcc tgccaaaacg cccgacatta
    42481 gcctgattgt cgtgccggaa ttgccgcaaa acaattcttt ttcaggtgct ttaaaaccgt
    42541 tttcggaatt aataaacaat tcaccgtcag agccgaattt cgtttcaatg cctaagttct
    42601 tcaaaacact caaagtcgaa agacaatctg cgcctttaga aaaatttttg acaacggatt
    42661 tgccttttac taaagacaaa attatcgccg ccctgtgcga aatcgatttg tccgcaggtg
    42721 caacaacttc gcccttaagc ccgagttgtg attttctgac ttctttttgc atagaacaat
    42781 tatactataa gttttggcaa atctttcaga gaataaaaaa ctgaggcggc ttttttaaaa
    42841 tcttcaggat ttgcacttac aaaaaagttt tcaaagcctt caattttact gtcatttatt
    42901 cctgtttcgt atattccttg tttaatagca tctgcaaagt aaacagaagg gtcaatgaac
    42961 atttctttag gggcaaattg agccaaaata tcaatcaaat aaggataatg ggtgcagccc
    43021 aaaattattt tatcggggtt gtttttgagc atttctttta catatttttc tattaaatta
    43081 atattttcct gcaaattttg agtttgattt tcgactatat taacccactc aggagttgct
    43141 atttcaaaga cttctaaatc agggttgtat tttttaagtt cttttgcgta aattcctgat
    43201 ttaatcgttg catttgttgc aaaaactccg actttttttg tctgaatttg agaaatttct
    43261 tttgcggaag cctgaattat gggataaatt ttaaacccgt aattatcctt aagtttttca
    43321 taaactgttg cagatgttgt gttacaagcc atcacaacag cttttacatc ttgtttttca
    43381 aaaaatttaa gaatattgtc aacaaattta taaagttctt cggatgtttt ttctccatag
    43441 ggaagatttt ttaaatcgcc gaaatagata taattttcgt aaggcaagag ttctttaacc
    43501 tttttaaaaa ctgttattcc tccgactccg gaatcaaaaa atgctatagg ttttttgttc
    43561 ataaatctct cttttatttt ttcccttggt taaggtattt taaaatacct tcggcaatag
    43621 cttgtgctgt tgcctgttta cgctgctcac ttaccagctg tgctctttcg gcgtcgtttg
    43681 aaagaaatcc tatttcgact aaaattgcgg ggacttccgt atgattaata acataaaatc
    43741 ttgatttgaa caagcctctg tcaggagatt ttattaagct catcattgat gaatgaattg
    43801 tttgagcaag ccctacactt tgaggttgat agtagtgtgt ttctattccg gtaatttcag
    43861 gttttgtgct tgaattgaca tggatactaa caaaaatgtc gggattgttt tcgcagcaga
    43921 taaaagttct gtctttaagc gaaaccgtat catctttgac tcttgtcatc aagaccgtaa
    43981 cgcctttttc gcgaagaatt gattcaaccc gctttgaaat atcaagggta atgtcttttt
    44041 cgtttatgcc tgcgcggata gcaccgtaat cagccccgcc atgtccggga tctatcataa
    44101 ctttatgttc accgctgatt gaaatttgtc tttttggtaa aatgtaagtt gttttttcgc
    44161 cttttaatgt tattttaaaa gcttttgcat ctgctcctaa gtcacagtta atcgcggttt
    44221 ctttttttag aggaagtgta agttttaaac ccactttagg aagaagttcc attttcatat
    44281 ttgcaaaaac acctgattta agagagtttt taaaatcaac atcattgtag cgaagggcat
    44341 tgtagaaggt tatttcaatc tttgaatcat ctctttgaat tcctgtcaca acggcagtgt
    44401 tgaatgcaaa aatatactcg cttgttgtcg gattaacctg ttttgaataa taagttacaa
    44461 tatcggttgt tttgctaaaa agcgatgtta aatcgacatt ttttgtgtca gatatcaaaa
    44521 ttgactgccc gtctgacgaa aaaacaggaa aatatttttc tttttcctcg gtggtaatga
    44581 ctattctaac cttatttgcc aaaaactgcc caattttgag cgtttcttta tcatttaact
    44641 ttatttcttt attgcgtatt tttggattgg caacagcatt cgggatgtca aaaaccaccc
    44701 tggaaggttc agtcaaataa agcggttttt caattcctat tgccccgaat ccgcttaaca
    44761 aaatcccgtc ctgttttgga taaatagcgt caagatagta ttttgacttc aatttaagtt
    44821 cttttttaac cccttcagac acaaccgatt taaccggagt taagctgact tgaggtgcta
    44881 tactggcatt aaaagccttt tgaatttggg ctaaaacttc atcctgcttt ggtgtgtcaa
    44941 cagctacttt aaccgtattt gcatcttcgt ctgaaattga caaatattca taaaaatcgc
    45001 tgcttgaagt tcgctcatca cggtaaatat tttggaaata atcttcctta caaataccgt
    45061 ctttaagttt gattacaatg ttattattaa ctttcaggaa agacactttt tgagcggaag
    45121 ccccttcgtc aaaatacaaa accactctca ccttgttcgg atttgtcgaa aattgtccca
    45181 ctttgacttg cttaagcaac cctgtgttaa aaaaccaatt ttgcccaggt accgttaaaa
    45241 ccgcagaatc aatatcaaaa taagcccttt ccggctcatg aagtttaacg agttttatat
    45301 tttttaaaat cggctcaacc gtgttgtcag gagaagttaa caatattatt gaatttgaag
    45361 tgtcaaaatt aaccgaagtt atcttgaaga cttcgtcaca aagcgcagat tgtataaata
    45421 cagctatgaa taaaaataaa agtaaaattt ttttaatcat ttttaaaaaa gtaaaaatcc
    45481 cctctttaga tattatacta ccaaaaagtg ccccaaacaa ttatgtaaac taatatttaa
    45541 tatattgatt aatttataaa acaagatata atatcaatgt aggcaagggg caaggggaat
    45601 ttttatgaac aagagatggt acgataatca cgacgaaaca cttcagactt ttgacattct
    45661 taaaaaattg gacagagaat ccagaaaaag acttgcaaac gatattgttg acgttgcaaa
    45721 tcagattaaa gctgttcaca gagaagaaga tgaaccttct cttactattg gtcttgaaag
    45781 agttttgggg atttggcaga gctctaatgc ccgtcgctgg tacgataaac aagaggactt
    45841 aaataccgca ataaaaacta tttcaaccct tccggaagac gattttctta atattatgga
    45901 aggaatttgc gtttcgctaa aggaataaaa atcaaatcaa aggagacatt ttatgaaaat
    45961 acaaagagtt accggtttac aagcggccaa taaattaagt aaaagtccta attttaatgg
    46021 taatttgtat ttgcagaaag gtataataga ttcaagtttg gagttttcca aaaaaaatct
    46081 tcattcttta tttgatcaaa ttatacctaa agattttttt gaaacatttt ataaaatgat
    46141 taataattta tcaaaaagaa tttccaaaaa agacgagttg ccaaatgttg tttttactat
    46201 atatcccaaa aaagaaggac ttatcatcga agggttaagg ggtgtttcaa atagaaacaa
    46261 tagaaaaaca gtcgaatttt tacctcaaga gtctttcagg aaaagactta atgatgcttt
    46321 tttagcaata aaaaaatatt atgatgattt tgataaagca gttaaaagct aatttattgt
    46381 taatatttgt aaaatttttg atgccaaagt agcaaacttt taaattttga gacattaaaa
    46441 caacgtagct tgtgtatttt tataaaaaaa ggagtattaa aatgcaaatt aacagagttt
    46501 caaatagcac atcttttgac ggtagattat tgattcccag ccacaaaaaa gccggagaaa
    46561 tggttaaata taccgagaga ggattacgtg cattattagg tgataacgtt tgtaaatatg
    46621 attgccaaga gtatttaagc tcactaaaca atcttttaag gatagttaaa aaagagtcta
    46681 aaaactttat tttatctttg tatccggtcc ctgaaaatgg aaatgtaaga tttattttca
    46741 gtcaagatgc taaattttct aaagttgttg aacaaccggc taatgacctt aaaatagttg
    46801 gcgaagaaat agccgtaaaa tataaaacta ttatgcaaga tccaaaacaa tttagtgaat
    46861 tggcttagat aaaacataac tatttaaagt cattattatt atttgaaaaa ttttaaataa
    46921 taaataaagt tgatttttta aagtaccgtt cttattatgc cgacttttaa aaaatgaagg
    46981 caaaggacgg tatttttatg gcaaaaataa tagaaaacaa agtcggcaga aggacaataa
    47041 gattatctgt cgatgatgtt ataagcacgg ttagagaata tcaaagaata gtcagaaacc
    47101 ctaaaactta cgaagggata agaattcttt taaaagattc ttcgatatat ttacctgagg
    47161 acatataaaa acacccgatt gggcaggtaa aataatagac aagtggtgtt attgtcaaaa
    47221 catgtttgtt gtatcctgta aataatagga gggtatttgt catgaaaaac ttttcaatca
    47281 tcgctttaat ttttgttatt cctttggtgg cttacatgat tttaaccaag cctgatgcgt
    47341 cttttgcaaa caaagcaggc ggcggggtta aagctcaggt aatcaaattt gcgtctccga
    47401 tgtgtcttga ctgcaaaaaa atggataaaa ttttaaaaga gcttcatcca aaatacgaag
    47461 gaagcattaa ttttatcgaa attcccgtac aaaacgatga cgaatacaca aagggacaaa
    47521 tagaaaaata caatgtaacg cttgttccga caatgatttt tgtcaacact aaaggacaaa
    47581 aagttaaaaa aatcgaagga tatattgata aagacgaatt agaaaaaatt atgaaagatt
    47641 tgattaatgg ataattacat ttcgcttttt tctcaaagcg caggtggcgg agaacatttt
    47701 cagctaattt tgctcgccgt ttcgtttttg ggcggattgc ttgcatcaat ttcgccctgc
    47761 tcgcttgcca tgctgcctat tattatcgga tatatcggcg gatattcaaa agaaagccct
    47821 ataaaaatat ttgttcaaat gatgtttttt gtattcggaa catctcttgt atttgctaca
    47881 atcggcgtga tttgtgctgt aacgggaaaa gtctttattt catttgctcc ggggtatttt
    47941 attttaatcc ttgcctcgct tttaatggta atggggctga atattttagg tattcttgat
    48001 tttaatcttc ctgttataat aaaacagatg ccgcaaagca atggcagaaa catgtttttg
    48061 taccctattt tgctgggtgc ggtttttgca atagcaggaa cgccctgttc gacacctata
    48121 cttgcgggta ttatggcgtt tgcttcattg tcaacaaata ttgcattggc tgttttaatg
    48181 ctgtttttgt tttctttggg gcaggggctt atacttataa tcgcaggagt ctttacctca
    48241 acgctcaagg gatttaaaag ttttgccgtt gtgtcggaaa ttttattaag attaagcgga
    48301 atcttacttt taggctcttc tttgtacata ttttacaaag tctttgcccc gttattgtaa
    48361 aaaaagctta tgtgtagtaa aattaacgca gatgaggcag ggttaatttt ttacccctca
    48421 cctcaatcct ctcccgcaag gggagaggaa gaataattaa ttttacaata cctagggaga
    48481 gaaactatgg ctaaaacttt tgaaggtaaa cttaccgtaa aaaacgaaaa attttgcatt
    48541 attgtttcaa gatttaacga attcataagt tcaaaattgc tttcaggagc agttgacgag
    48601 ctttcaaggc acggggttaa ggacgaaaac atcagcgtta tatggtcgcc cggagctttt
    48661 gaaattccgc ttcttgcaaa aaaatgcgca aaaacaggaa aatacgatgc gattataact
    48721 ttaggcgcaa taattaaagg tgcaaccgct cattttgatt atgtcagtgc agaattatca
    48781 aaaggaatag caagcgtaag ccttgaaacc ggagttccgg ttattttcgg ggtacttaca
    48841 acagataata tcgagcaggc aatcgaaaga gccggcacaa aatcaggaaa caaaggttct
    48901 gatgccgcaa aagccgctat tgaaatggca aatcttgttc aactgatata atttagggat
    48961 aaattcgggg aagttatgag tgaagtaatt acagaaacgc gaaatctctt aacaagagat
    49021 atcgacatta tttctaccag agaaatggtg caaaaaatga atgacgagga caaactcgtt
    49081 gcttttgccg tagaagaagc acttgacgaa atatcacaag ccatagatat aatttcaaaa
    49141 caatttttaa aaggcggaaa actttactac ttcggtgcag ggacttcggg aagattaggt
    49201 gtattggatg catcggaatg ccctccaact tttagcgtgg agcctgatat ggttcaagga
    49261 attatggcag gcggcgacag cgcatttaaa acagccgttg aaggtataga agataacttt
    49321 gacgctggag tcgaagatgc aaaaatttta acagaaaaag atgttgcagt ggctatttct
    49381 gcaagcggaa accctaatta ccttttaggt gttttggctc aagctgaaaa agtcggagca
    49441 aaaacagtcg gaataacctg caacccaagc ggaaaaatat ccgaagaagc agggctggta
    49501 atctgcgttg atgtaggacc cgaggttatt gcaggctcaa gcaggctaaa agcaggaact
    49561 gcgcagaaaa tgattttaaa tatgctcaca acaggtgcaa tgataaaaat cggcaaaact
    49621 tacgaaaatt ttatgattga tcttaaggca agtaacgaaa aacttaagga cagagcaata
    49681 agaatagttg ccgaattagc acaagtaacc catgccgaag ctttaaaaaa acttattgaa
    49741 tttaattggg aaattaagcc tgctatagtt tcactttacc ttgcaataga tatcgacaag
    49801 gcaaaagaac agcttaaaaa gcacaacggg attttaagaa aggttattta agctattaaa
    49861 tctaaattgc ccagctttgc ctcgatttta tcatcaaact tattataaat gtttatattt
    49921 ttaacttcag gtgaatacgg gttttttaaa tctaaaactt taacattacc ttttctatca
    49981 gggtatgtta aggctattgc tgaaaaataa tgattttgtt caccgttcat tgctttttca
    50041 tagttatcaa ctcttactat atccatagga tgaacgtctt ttgggttaaa cgcgccgtga
    50101 tgaggtctat acttaacaaa aggttgattt gagcaaaaat taatatcgtt aatcatgaat
    50161 ttctcctttt acaaaaatta agctgttatg tctaagcttc cgagaacatt aacagggcga
    50221 taatcagaat tattcaagtc tatgtgaaca atttcgcctt tatcacgtcc tttaagaatc
    50281 gcaacttcag cctcgttgcc atctttgctt aaaattattc cgggaatata ttcattaatc
    50341 cctcttgcgg ttaaaggttt atattcaaaa tcaaccacat ctgatgaatt aaattcatta
    50401 ctattttcac tgctttttac aaaatctatt ttttgaggcc ttgatttaaa agaaacagaa
    50461 ctaacattta aattcatata tttactcctt aactaattac gtaaatatta aaacctctaa
    50521 aaaatatttt tgctaatatt tataaaaatc gttacaaatt taaaaattgt acaaatattg
    50581 acgttcttga tataattaaa atgtcatccc gcactacgtt gcgggatcta atcaataagc
    50641 atatttaaac gagattgctt cggtgtataa tttttattaa gccgaaaatg aatgtctcgc
    50701 aatgacggag gtaaacaatg aaattaacgg ttttaggccc gggatgttgg ggattaactc
    50761 ttgcgtggct gcttacaaat aatttcgata aaataaccgt ttggggcagg caagaagatt
    50821 tgtacgaaga tttaatcaag aacaaacact gctcaaagcc acttgaaatt cagcttgatg
    50881 aaaaaataga aattacatcc gatttatcat ctgcaataaa agactcagaa attatacttc
    50941 ttgttgttgc aacttcaggg ataagacctg tttgtgaggc tttgaaaaaa tctggaatca
    51001 aaaaagaaca aattcttgta aacgcttcaa agggaataga attaccttct ttgatgagaa
    51061 tgtctgaggt tataaaagaa gttttgcccg aacaaaaact tgcaattctg tcaggtccga
    51121 ctcttgcaaa agaagttcta cttggcaaac ccactgctgc gtctgttgcc tgcgaaaatt
    51181 tggaaacggc aaaatttgtt cagcaggcat gcaatgtctc gggacgtttc aggctttaca
    51241 caaacaaaga tgtaataggc gttgaacttg gcggaagcct taaaaacgta atcgctatag
    51301 catcaggctt tgcccatgca atgaatttgg gcgacaactg catgggggca cttttaaccc
    51361 gtggaatggc agaaatagta agagtaagcg taaaattggg cgcaaatcct tcaactctat
    51421 acggactttc cggcatgggt gatttaatag cgacttgttc aagcccgctt tcaagaaatt
    51481 acacggttgg cgcaatgctt ggcaaaggca aaaaaattga cgaaatttta agcgaattgg
    51541 gctcagttgc agaaggtgtt aaaacttcaa aggcaatttg cgaacttgca aaaaaacttg
    51601 atattgaggt tcctgtatca gaagccatat atgacgccgt ttatactgat attactccga
    51661 tagaagtctt gggtaagctt atgaacagaa agcttaaaga agaagaatca tatctgtgat
    51721 agaataaaat tatgaaatat gctgtaagat tattgaaaaa tactttccac cctttgcaag
    51781 ttcccgaaac ccttgaactg gaacatggtc agatggtttt ggtcagaact gaaaaaggcg
    51841 aagaagcact aaaaactttc ttggtcaatc cagaaattgc ccaacaatgg gaaaaatcaa
    51901 agcctgaggc tcttcctgtt ataagaactt tatctcaaag agatatgcaa actcttgatg
    51961 aaattaaaca agaagaagtt gaatctttta acaaatgcaa agaattggtt aaaaaacaca
    52021 atctgtcaat gaatttggta caatgcaaaa taacttttga cagaagaaaa ataacatttt
    52081 attataccgc ctcagaaaga gttgatttta gagaactgtt gaaagattta acacaaactt
    52141 ttaaacgcgt aagaatagac ttaagacata tcggagtacg tgatgaaacc tcaatagttg
    52201 aaggcaacgg catctgcgga agaccttttt gctgctgttc ttttttgaga aaatttgatt
    52261 caataaacgt aaaactggct aaagaccaag gcatgccgat aactccgggt aaaatctccg
    52321 gaacctgcgg aaggctttta tgctgcctga attatgaata ttcgaactac attgaggctg
    52381 caaaagatat gccgcctgtg ggttcaagtg tcatgacgcc tgatggattg ggcaaggtct
    52441 tttcacttaa ttttcttaac agtaaattaa gtgttaaact cgaagacgga aaaattaaag
    52501 aatataaaaa agccgagctt gaaatggttg atgcagaagt aaatatcgaa attgatattc
    52561 ctgttgtagc ataccctgaa gaaacagagc atgtggatat ttcacagctt gaagatgata
    52621 aaaacagttc aaccggaaat gtttaaaaat ttttaataag attaaagggg cggatttgct
    52681 ttgcaaatcc gcccctttaa aaacactatc ctacgcgaat atattaattt tttcttcggt
    52741 ttcttttttt tctgctttac gaagttcacc tttataaaaa ggatttgagt tgtttttgtc
    52801 tttgtttaag tcagttgcag gtttaaccaa ttcggtaaat ttcggaagtt gataaacatt
    52861 ttttgtttca ttaccttttt cttcaacagg aagagcgatt ttaccttcaa catttttaaa
    52921 tattgccttt gaagcttcgc tgtttaagaa ttgaaggctt ttaacagctt tttcgcttaa
    52981 agaaacctga actcctgaac ttgccattga aatttcacga gcagttgcag cgttgacttt
    53041 accggcatac aaatccaatc ctaagtcagg ctttttgaat ttgctgaaat ccaaatttga
    53101 caactcggaa gatttagatt cggccctttt gaaaattgct tgcgtaacat caacaagtgc
    53161 ttttgaatca attcctctat taatgctttg gttgtaagat acattaagac tcatgacgat
    53221 atccctttat cccttttttc gtaacttttt cgtaatccat tccttatagt tgttttatcg
    53281 gttgttttta aagcaaactt aagaacttaa gagaaaatat tacaaaaaat ttacaaaatg
    53341 aagaaaaaag ataataaaaa cagggcggat ttctgaaaga aatccgccct gtttttattc
    53401 atatttaaat ttatttttta caatccggtc cttcgcattt tttaggctca gtttcttgtt
    53461 ttggctgaac ttcaggtgct ttttccgttg tttttgttgt tatttgtacg gtatctgttt
    53521 tagattgttc tgcggcaggt gcggcagcag ttgtttgtgc tacaacagtt gttgtttcag
    53581 gttttttccc ttcgcctctg aacaaagtgt aaacagatgg tctggggcaa caattaaaca
    53641 ttactttcat ttaaaaaaat ctcctcaacg atatctaaca aaataattat aaacttaatg
    53701 ataaaaaacc acaaataaaa tcagattatt aatttacaaa tctaaacagg atgaaactat
    53761 actcaaaatc aaccgattag gtgatgttca ttttttaata ataaacaaaa tagaataagg
    53821 aggaatgaac atgcaagtaa atgattcttt cgtacaggaa acctttgata taagaaaatt
    53881 atggaatgaa cagcatccca taatccacac ctggtttttg ttgagttctg tttcaatttg
    53941 ttttttatct gcgatattat ttataatccc ttaaaaacta tccgattaac ctatatggga
    54001 gaataatgtt aaaatttctc ttctgattta tgctgaagtg ttaaaattag aaggagagga
    54061 aaatgaagcg tgcggtaatt ttactcgtat tttacctgtt tggcacaaat tcggggtttt
    54121 gtgatgtaac atattttcct ccgcttcagc ctttgggaac aaatgcaaat aatgcttttg
    54181 ctcttagaaa ctacaatcag gtgaataacg ctgattatca aaaaataact cagatagaaa
    54241 attcacttta cggcaaaaat tttgcatatc aagatatctt gataagactt tcccgaatag
    54301 agaaaaatat ttttaacagg acgtatccga atttatcttt gtcacaaagg gttgataata
    54361 tagtcgcaaa ttataatcaa actacacaat atccgaatat atcaaaaaac accttaagca
    54421 aaatggaaat gaaaatattc gggcaaaatt ataatggaga tagtcctgaa gttagaatcg
    54481 aaagacttga acaacagctt ctcggagcag tacaaagcgg ggacataaca acaaggtaca
    54541 caacactaaa aacattggcg ggtaattata attcaaatca atactcacaa aattattatt
    54601 ctaacccgat tgtaggcacc caaggcggat ggaagggggt attagggagc cttgggggca
    54661 tgctccttgg tggaaacggt atgatgacag gatttacccc acctatagac cagtattacg
    54721 gttattcaaa tccttcatat cttaattatt cttcgttggg gaatcccgga tacgggtatt
    54781 acaacggata cagaacaaac cacggatatt acgatagatt taataattac ggctcacaaa
    54841 caggtgttac cattttagat taaataaaaa gggcggattc gctttgcgaa tccgcccttt
    54901 ttcaaattta ttttaaccta ttttaaaatc ttccatagcc tgattttgag ggtaattatc
    54961 tttcaatgtg tcaaagtttt ttaatgactc tgagcgaagt ttttccatat aaacctgacc
    55021 gtttttaaca acttgttgaa gttgaattaa gtcgtgttcg atatttgtta aaacttgttc
    55081 tgcgtagagt tccgcacctt ctcttattct tattgcttcg tcagctgctt gaactcggat
    55141 ggcttcagct tcagaaagag cttttctttt aatatcttca cattctgata taacctgttc
    55201 tttaattttt tcagcttctc tttgtactgc gcgaaggaca tctgattcac ttaaaagcct
    55261 gccggcttca ttttgagcgt caaaaattat tttttcggct ttttgctggg cttctatctg
    55321 gagttcttct ctgcgcctta agaaagcacg ggcttcttgc acttcggtag gcaatgaggc
    55381 gtaaattcta tcgattaaag actcaacttc ttttacttta attgcaacca aataatttgg
    55441 aagaatcgga aagctttttt ccaaaacgat ttcaagtaat tttaagagtt catatatccc
    55501 gctttgttgc atattcataa tcaacgatac ctttcttact atattctata acaataaatt
    55561 ttatttggca aatctcaatt ttaaaaattc ttcaaccggt tcggtaacga atttagaaac
    55621 atcaccgtgc attcgggcga tttcttttac ggtacttgaa gaaataaagt tatacttggg
    55681 tcttgttgtc aaaaaaaccg ttttaatatc acccgcaagt gacctgtttg tttgagaaag
    55741 ctgcatttca aattcaaaat ctgatactgc tctcagccct cttataataa cttttgctcc
    55801 ttttgatttt gcgtattcaa cggttagtcc gtcaaaggaa tctacttcaa cgttattcaa
    55861 tccatttatg ctttttttaa ttaagtcaag cctttgttca aaagtaaaca aagatttttt
    55921 ttcgctgttt ttagaaaccg caattataac cttttcaaat atttcagaag cggttttcaa
    55981 aacatccaaa tgtccgttag taatgggatc aaagctaccc ggatatactg caatcgtcat
    56041 atttttcctt taaaaatagt atctatctac aattatataa tcataaaatt aaaaccccta
    56101 ataacaggta attttttcat tacaaaattt aacaaacaga taaatgtaac aaaagcttaa
    56161 caatatttta ccttgttttt caatgttttc aaggtaaaaa cagtattttt gtttattaat
    56221 aggcaatttt ttaaaatcaa aagagtttat gtatataagg ttagttttca tcttggaggt
    56281 tacatggtaa acagtgtaag ttttggtaat agttatggga attttcaacc gcaaggttat
    56341 gtttcaccat attatttaaa aaaagccgaa gatacagcaa aatatactac tgctgcagtt
    56401 aatgcccaaa gtaatgaagg cggacctttc acaggagtag gcctttggag cggatttatg
    56461 ggtgcaggct ggctatggcg aaatagaaaa gacatccgcg gaggctttaa tacgcttaaa
    56521 gtaaacggac aaacacaagc agaattattt aaggcagatg gcggactttg gagcagaaat
    56581 gcttataaac aggctttttt aagatacgaa actgaagctg caaaagctgc taagttaagc
    56641 agtgttgatc aatccattaa aaatttaaat aaattatctg ctcaagctaa aaaagcaggt
    56701 gttacttttg aagaatatgt tgctaaaaat gccggtaaaa caggtatgtt gtcaaaagcc
    56761 ggtgatttta taaaaaatac aaaagcggta aaagcagcgg gaaaaatccc cggattatct
    56821 aaaatagcta aactgggtaa aggaaacgga ttctttttcg cgatatcagc agcattagaa
    56881 ttatttactc aagttattcc tacattcaaa cagttaggcg caaaagccgg ttggaagcag
    56941 attggaaaat caactgcgac aactatcgga acagttggag gttttgtcgc aggacaagcc
    57001 gcaggcgcag cagccggagc tgcaataggc gcggcagtcg gttcagtaat tcccgtagcc
    57061 ggaacagcag tcggcgcagt tgtaggactt gcttgcggat tcttaggctc ttggcttgga
    57121 ggaaaagcgg ctaaagctgt tgtaggagaa aatgaacttg ataaagctaa aaaagaagga
    57181 agattaccgg cagatgatgc tcaaccagaa gttgctgcac aaactcagtt gccggggcag
    57241 aatgaatctg ttggtcaaat tgctcaattg ccgcctattc ccgggttgga aaatcctttg
    57301 atggaaaata acagtatgcc cccatttact gcgatgcctc aaccgcaggc tcaacaacag
    57361 cctcagcagc aagtgacggc tttcggggta aacaatcctt ttgcagcaag aaggcaaaag
    57421 caatttcagc caaatagcaa cccttttgga gcagggatac aaaaccaaat gtcaagctta
    57481 ccttatgcag aaagggattt tatgtcaatg acagcaggat tggcctagaa taagtattta
    57541 ataatcgggc gaagatttca tttatgaaat cttcgcccga ttatttgtat aaaagttgca
    57601 ttttttgaaa ttggaattac aattatatat atagcggagg agagaaattt atgaaaacaa
    57661 agattttaat attgttgtta tcagttatgt tcggggtaaa ttcagtttat gcagcacctt
    57721 ttaaggccga tgcaaagacg aaaagaattc ctgcgggcac aaagttaaaa attgagctta
    57781 tggattcttt atatacacaa gtcggaaatc aaggagatgc ttttaatgca atgcttttag
    57841 aagatcaaaa atcgcagaca ggagttattc ttcccgcagg ctcaataata agaggaagta
    57901 taaataaagt aaccccgata aaaatgctat ccagaggcgc ggttttgtat cttgattttg
    57961 atcacgtagt aacacccaac ggaagacagt tgccattatc tatgggcgtt tgcaatagag
    58021 caaaaataac agatgacggc ggtttatacg aatcaaaagg atatggtgaa gccttacaga
    58081 aaaattggca aaaaacagtt gaaataacgc aaaatgtaac tcaattcggg attgatgcgg
    58141 gtgagaattc atttaacggt gctgtattta taaccgctcc tgtttgcgca ataggcggag
    58201 caatgggcgg aggtgcttat ttgttgggcg atagcataat agatttattc agaaaaggtt
    58261 cggatgttaa attaaacaaa ggaacggtac tggatgtaat gctaactcaa ccgattgatg
    58321 tccctgtaaa ttgacgcagt tctaggcgag cgaagcgagc cgtagaaatg cgtcatcctg
    58381 aaacattagt tatcggctgt gcattggccg tacttattca ggatctcatt gtaaaccgga
    58441 tttttgtcat cccgcatgaa caaagtgaat tgcggaatct cacgtgcaaa tagaactaaa
    58501 aatatcatcc cgcagactac tgtcatcccg catgaacgaa gtgaattgcg ggatctaata
    58561 tactgtcgtc aaaagtggta acggtaaaat attgccatta tcgtacaagt taactaaatt
    58621 aggattaaca tctttaatta aattccattt ccatgctctt ttccaacgct taatagtttt
    58681 ttctctcgaa attgcttcca agatatcata atgaatttcg taaaataata gctgctttat
    58741 atggtatttt ttggtaaacc catcagctga ttcgttatta tgctcataag ctctttttat
    58801 aagatcggaa gttacaccgg tataaaatac tgttctatta taattagtta aaatataaac
    58861 ataatattgt ttcatgtgat tagtttaaca tatattttat agatcccgca acaagtgcgg
    58921 gatgacagat ggtgtgcggg atgacagatg gtgtgcggga tgacaggtgt gtgcggggtg
    58981 acaggtgtgt gcgggatgac aggatggggg cggggtataa attttacgtg ataatataaa
    59041 gaagcggcgg atctttcgac ccgccgcttc ttttggaata atttccaaca agctcaaata
    59101 ttattaaccg ttgataagtt ttaatgcaat acttggagct tggtttgcag ctgccaacaa
    59161 tgaagctgaa gctgactgaa gaatttggtt tttaacatag ttagcagatt cttctgcaac
    59221 gtctgcgtct ttgattgttg aatttgcagc tgttaagtta gtttgttgta ctgtcaagtt
    59281 tttacctgca gctgacaatc tgttttggaa tgcaccaatt tttgtttgtc tgtttgtaat
    59341 gtttgtcaaa gcagttgtac agttagcaag gaatgcttta gaacttgcag cactttcaaa
    59401 cgctgaatca attgatgtac caccggcagg tgttaactgg atggcagata cggttgcttt
    59461 cgcaaaaaca tcagaagaaa tagtaagtga atcatccgct gaacttcctg tacctacttg
    59521 gatagctata cctgatgact gggttccatc taacaattta attccgttaa atgatgtgct
    59581 gttagccaaa cggtcaattt ctaatgctct ttgttgtgct tcagctttca ttgccgctct
    59641 atcatctgca ctataagttc cgttagcaga ttgttctgcc aggtctctga tacgttgcaa
    59701 gtttgattta ataactccta aagtaccttc tgttgttgtt aataggtttg aacctatttg
    59761 aacgttttta gatgctactt ttgaacttga taaagtagtt tcaaatgatg ttgaaaccgc
    59821 caatccggct gcatcatcgg atgctgagtt gattctgaaa cctgttgaca ttctcaacat
    59881 cgatttgctc attgcatcgg ttgcatcatt caagttgttt tgaatgttga ttgccgataa
    59941 gtttgtgttt acagtaattg ccatagttga tctcctttca aagtgacctt tttacttcct
    60001 tgtgtttact tccttgtgtt tacttttttt gactgctagt ctgcaaaaaa taagatccaa
    60061 aaatacacgc acaccgcgtt aaggttgatc ctttttgctc actcacattc atactgttga
    60121 ttaatctaag ccagtgctaa taactaattt aagttacatg cataacttga cttcacatac
    60181 acatattacg actattctga ggactaatta attcagattt agtttgaaat atctgatact
    60241 ttagtataaa tcttttaaaa ataaatttaa agagggtaaa agtgtatttt aaacatttct
    60301 taatataaaa aaacaaacaa aacttgacaa agtcaataga taaaagggtt ttaaagtata
    60361 tatgttttat attttattta actactaatg taaattttta ttacaattat aaagttattt
    60421 taaatttttg gcatggtttt atagtgttga gtttagaaaa aaatggggcg aaaattttta
    60481 aaaattttcg ccccattttt ttctaatggt ttttcttatt aatataggtt cattgaaata
    60541 actttttgag caattcttac agtatttaaa gctgcgccga taagcagatt atctgccaca
    60601 caccacagag aaatagcgtt ttcaaaagca aggtcttttc tgattctgcc gacataaaca
    60661 ggatttttgt aagctgcatc aatcggtgtg ggatattcat aattttcaat gttatcaatg
    60721 acttcaatac cgtaggcatt ttttaacagt tctttagctt ttttaggtgt tatttcattt
    60781 tcaaattcta tggtaacggc ttccgagtgt ccgcgataga caggtacacg cacggcagta
    60841 caggttatcg gaagactttg agacaaatgc agtatttttt ttgtttcatt aacaactttc
    60901 atttcttctt ttgtataacc gttttccaaa aaagagtcaa tctggggaat aacgttaaat
    60961 gcaatcggtt ttttaaaaac tttattttca aatgtttttt catccaatgc cgcttttgtg
    61021 ttttcggtta attcattaat cgccgcaatt ccggctccgc ttaccgattg ataagtggaa
    61081 acaatcaggc gttttatttt ggcaaaatca tctaacggtt ttaagacaag cataagctga
    61141 gaagtcgagc agttaggatt tgcaataatt cctttgtgca gttttaaatc ttcatcatta
    61201 actccgacaa taacaagcgg aatatcttta tccattctga aggcacttga gttgtcaata
    61261 aaaacacagc cttttttaac agcttcgggt gcaagtgaaa gacttgtaga gccgcctgcg
    61321 gatgcaaggc agatatttac ttcctcaaac gattccggct tagcttcttc gactttataa
    61381 attttaccct tgaattccat ttctttgcct gcgcttcttg cactggagag aagttttaaa
    61441 ttattaaaag ggatatcaag ttcttccata acttttaaaa tttcacgccc gacaacacct
    61501 gtggcaccaa gtattgctat atttggtttg ttcatttgtt tccttccttt gcacgtcatc
    61561 ctgaacttgt ttcaggatct cataggcgaa agactttgcg atgctgatac tattgaaaat
    61621 tttaacatga attggtctat ttctgcaagc cgtaaggaca ttttctacgg ctcgttcctc
    61681 gccttgaaaa gaagtcaaat aaattcagca tgacgtaata attaactata atatattttc
    61741 aagtccataa acaaagtcgc tattttgtgc aacgtgtttt attgccaaaa tcacgccctg
    61801 catatagcaa gccctgtcca tcgtatcgtg gcgaagttta aaaatctgcc ctgatgcacc
    61861 gaaaataact tcttgcgaag caatgtagcc cggcattctg acagagtgaa tatgaatatc
    61921 agaataagca acgccgccac gagaaccttt tatcagctca acttcttcgc agttgcccgt
    61981 tgcaaaagtc ttattctcat ttacccccgc aatcatttga gcggttttaa ttgccgtacc
    62041 tgaaggtgca tctttctttt gattgtggtg aagctcaatg atttctgcat tatcaaaata
    62101 ttttgcagcc tgctgcgaaa acatcatcat caaaaccgca ccggtagaaa aattcggcgc
    62161 aattaagcaa gaaacatttt tttcttttga aatctttttt aattcttcga tttgagcatc
    62221 tgacaatccg gttgtaccga taactgggcg aattttgtta ttcaagcaaa attttgcgtt
    62281 atcaaaaatt gatttcggct gggtgaaatc gaccagaacg tcgatttcta cgcttttaat
    62341 cgcatcttct atgcttttaa aaacactttc tcccccgcaa atatctattt ttgcaacaag
    62401 tttcatttca ggattttcgc ctaccgcttt tattacttcc ttgcccattt tcccgcaggc
    62461 accgcacacc gcaattttca tcatgtcagt atcctctttc ttcttaacaa ttatattata
    62521 aaaattaatt atgataaaat tgttccatga atactttttc ccaaaaaatt ccaatagcta
    62581 aacaggaagc gataaaaaag tttatcttgc aaaactttca ggcaatagtg caccctaagc
    62641 aatatacctt ttgggagatt aaaaataagg attttgtcgt aactttttat aattcaggca
    62701 aatttgttat tcaagggaaa aatatcgaca tattgcttga tgttttaagt tcaaaatttc
    62761 atgaatttag tgaacaggaa atagtaaacg gtaaacacaa taccatggca actccgctca
    62821 ctgttcatag ttcaccgctc acttatatag gcaccgacga atcagggaag ggcgattttt
    62881 ttggtccact ggtgattgcg ggcgtgctta ttgatgaaaa aaatcgcccg atttttaaag
    62941 atttgggcat taaagacagt aaaatgctta aagacgaaaa tattttaaaa atggcaaaac
    63001 aaatccaaaa aaacgctgta ttttcaacag ttgtaatttc aaatgcgaaa tacaatgagc
    63061 tttataaaga atttaagaat ttaaataagc ttttggcgtg ggggcatgca agggttattg
    63121 aaaatatttt ggaaaaaagg ccttgtgaat atgcgctttc tgacaaattc ggtgatgaaa
    63181 gcttaatcaa aaacgcttta atgaaaaaag gcaaagttat cacccttgaa cagagggtta
    63241 gggcagaaag cgatattgcg gttgcggcgg catcggtttt ggcacgggca acttttgttg
    63301 aaaaaatgca aagtatggaa aagttttacg gaataaaatt tcccaaaggc gcaagcgaaa
    63361 aggttaaaga cagcgcaaaa gaattttccc gaaaatatgg gagagacaga ctaaaagaag
    63421 tttgcaaaat ccatttcaag acttataaag agctttaaat tcatttgaca taagtatttt
    63481 tttatattag catatagctt aagtccaagc ctttatgggc ctcggtggcg gaatcggtag
    63541 acgcgttgga cttaaaatcc aatcaggtta attacctggt gccggttcaa gtccggcccg
    63601 aggcaaactt ttaagagtgg gttatctaaa tggatagccc ttttttaatt tcgaggtaaa
    63661 gatgacggac aataaaaaga aattgacaaa aaagaataag aaaaaagctg tttttaatgg
    63721 ggttaagggg cttaacattg ataaaaacga atatcaggaa atagttttgt cgaacacgaa
    63781 ctttccggat aaatcttttg gaaaccggct gaagtagaag ttatagttgt ttgcttgcaa
    63841 aaaattcgtc agcatgcaat aatgatattg gaagtatata aagcgggata ctctgtcggg
    63901 ggtaaatgcg ataaaagata ctgaaacttt aatacaacgc gggatactct gccgaaccaa
    63961 attgactaaa tgtcaatttg agtgagaggt aagtctggaa gactgattag tgagttgagc
    64021 gtcttgataa aagaataacg cgggatggag cagtctggta gctcgtcggg ctcataaccc
    64081 gaaggtcgtt ggttcaaatc cagctcccgc aaccattac
//
LOCUS       DAOQAU010000005        60477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_361_length_60477_cov_7.532819, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000005 DAOQAU010000000
VERSION     DAOQAU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 60477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60477
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_60477_cov_7.532819"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PKI94_01530"
     CDS             <1..842
                     /locus_tag="PKI94_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNW25451.1"
                     /translation="IVDLSEEFSLVTCCDGIGSKIIPLYEKKLFKIISADVVAANLND
                     LVCTGAKAVGFVDYIAANKLDSNEVFSIVEAIKKELDNCGCKFLGGETSEINELITKG
                     NIDISGFAVGVVKKEEKLDKNNVKENDVVVGLCSSGIHANGFSLVRKLYAQKKLTDKE
                     FETCLAPTCNYYNCVSKLVEKKLVKSCANITGGGIFSNLIRAIPDNLSAELDFEKIPP
                     QSIFQKLKSICGDEFFEVFNAGVGFCMIADEKNLEQIFEISKEFNPFILGKIIIKNKI
                     ITQ"
     gene            944..1546
                     /locus_tag="PKI94_01535"
     CDS             944..1546
                     /locus_tag="PKI94_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNW25452.1"
                     /translation="MTKKLAIMGSGNGSNFEAIAKFLQGKDVEITCLSDVKDAFILKR
                     AQNLGISHEFLSFEKNYEYFSKNKFDLVALAGYMKILDEKTLKKMGNVINIHPSILPS
                     FRGKGAIKKAFLAGVKLSGVTIHWVEKEVDKGKIIAQYPVFIENAMSLDEFEQAIHNV
                     EYKLYPIVIEKLLEDKVFDFQELITPTSNKKCGGCGGCQN"
     gene            1534..>1832
                     /locus_tag="PKI94_01540"
     CDS             1534..>1832
                     /locus_tag="PKI94_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HNW25453.1"
                     /translation="MSELNQKLNVLVLGSGAREHSIALSISKSPLLNKLFLAQTGAFE
                     LGEIIEFRDFEDLAKKCVLKKINLAVVGPEEPLCEGIVDVFKKYKIPTIGVDKYFS"
     assembly_gap    1833..1932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1933..2649
                     /locus_tag="PKI94_01545"
     CDS             <1933..2649
                     /locus_tag="PKI94_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNW25454.1"
                     /translation="GKNAQTILIEEFLQGEECSLISLWDGKEFLHFPPARDFKKLDNS
                     KNAPNTGGMGAFCPVLLNKKQQTKLDEYKKQLQNALKKEKAGFAGFIYSGLIWAQKDK
                     IWDWYVLEYNVRMGDPECQAILTHLNSDFLEILFKASKGKLKNLKPSYKKNTSACLVI
                     ACSGYPQKPKDGEEIVLPEDKKIQVYFAGVEKKGEKLFSKGGRILSLCTNAKEPFSIL
                     KEYAKTIKMKHKYFREDIAL"
     gene            complement(2627..3100)
                     /locus_tag="PKI94_01550"
     CDS             complement(2627..3100)
                     /locus_tag="PKI94_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25455.1"
                     /translation="MRDINFQNLLNLKERSNLRLVWLILLCFSFCICLSGCRKPHTII
                     LFNHSPISKENFLNSSVNFKRDERIYYLFISEKPIKSDYIRIQVLKKDEKSEWWGTKI
                     AYANDFKVYKDQVYYYNDYIVLNEAGYYYMRVFAKNNLETPLATASFYVREQYLP"
     gene            complement(3123..5156)
                     /locus_tag="PKI94_01555"
     CDS             complement(3123..5156)
                     /locus_tag="PKI94_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25456.1"
                     /translation="MKKKLLASAIILALTTFPAQARMTKEAHSLYQQACTLEYQKKYE
                     GAITLIQKAIEISGEDAMLYTKMAGLYADMGDLQNALASYKKAIHLRPNDAFIYISIG
                     NILQTQGDYENAYKSYKQAMDIFPDYKYNYLNMANVQYYMGENKEAIDNFNKFLAIYP
                     DNQDSRINLAGAYLKDNQIEKACDEYNALYAKNPADFDQYASYGMALFKAKKYDTAID
                     ILKKAVEQNPEDVVSHANLAVSYQQMDKDDLALTEFEETFKLKPDFTSLRFDYANLLA
                     DMGKDDAAIEQYKIFLARYPEAPNAYANLATVYKRQNNLDLAILNFTKSLSKDNTNIA
                     VKKELALCYHTKGDYSNALNYYEEVLATDKGDYDIKANKALVLHAMERYDSAIPTYKE
                     LLAQKDNPRLKINLSSALLSQGDILMKNSDYQKAASSFYEAIIYNPNEAYAYFSLAKA
                     YENLGYEDKAIENYEKAIEIDPENQDYRLSHKALVDKNKPKEEIIEQTQPQVFGQQIE
                     TEVQTPAAMPKIVEPQVEDVQTLIKSGDALYNQKRFEEAIVKYSKALDLKSDDSVTCL
                     KLGNLYKLKNNPDKAVFYYKKAIEIDPNYTDAIFNLGLVYAEGSNYTESKKCFEKVVY
                     LNPNYTYAHYALGLAYETEGNKVKAIQSYEKFVGLEKDPKIRQAVEAKIDSLK"
     gene            complement(5292..7226)
                     /locus_tag="PKI94_01560"
     CDS             complement(5292..7226)
                     /locus_tag="PKI94_01560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW25457.1"
                     /translation="MNKNKKNSCPSLKILIFASIIFVIGVAYITWRWIFTLNLLTSPF
                     TTFWSYALLAAELVTLIVFTNFSMILVKASNLEKTEPDNVLQQDYSKDIFKNGELVTF
                     KNFCPSVDIFICTIIESPDLLATTISACKNIDYPNKKVYVLDDGRRPEIKELTELLGC
                     NYITRDTNKGFKSGNINNGLSQTDSDIVVIFDADHMPASTFLRETVYFFTDENMAMVQ
                     TPQHFCNPDAFQKNLKMEKFLSNEQEMFYRVIEPALNEYGSVFCGGTNILIRRTHLAS
                     VGNFPENSITEDSLLGLKFHAAGYGIAYYNRPIAIGVAASTFEEYIKQRCRWAKGNVQ
                     ILTTPKNWEYYKKLTPIQAFFYLSGVMYFITPIARIIFLLAPVLFLFFDITPVLILFY
                     QIFAFQICYFTIKFAFIFNQCVRIKNVILADVYDLITSIFTIGSILQALFIPEKLAKF
                     KFIVSKKFSKSTKTNMKYAVPLAVIFGILIAAQLEGIYDILYSDVYSVLAVIANLFWN
                     SINIIVLFFGIQVVTEKPEKRTYQRVNLEENLNIEDDKHKKYQTIVEDASRSGLKFLA
                     DENLEQKAQNEFTTKIGNEESPLKILSCSLEKGKYLCKSMFKKPIALFKLTRQNVNRL
                     DNYVKFAYKKPDKWDDYFED"
     gene            complement(7219..8337)
                     /locus_tag="PKI94_01565"
     CDS             complement(7219..8337)
                     /locus_tag="PKI94_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNW25458.1"
                     /translation="MQKINLLIFGVIVSLVALFILKAPSKEPAFCLKDSYDFYKIYYM
                     TKDGRIMDPQRDNITTSEGQSYMLLRSMIMNDFKTFDLVLSWSQNNLQRNDKLFSWLW
                     GENKTGDYTILDKNSASDADIDIAFSLILAYEKTKNRKYLKTALPIINSVWENETKIV
                     DGKRILMPGVNQTLVEKVEVNPSYFSPYAFRIFQKYDKKHDWDELIKSSYYYLEKSME
                     KTKTGLPSDWFLIQNGQVVLENSTRSDFSYDAIRVFARIFLDNEKTGDKRAFKILKNA
                     DFFTENWKKSGKIYVNYTAEGKLKNKEEFAGSVAILIPVINMSDEKIAREIYTEKLVP
                     IFKDKGYWDSTSDYYGQNLIWFGCFLYNCDKTHGNLDE"
     gene            complement(8324..11212)
                     /locus_tag="PKI94_01570"
     CDS             complement(8324..11212)
                     /locus_tag="PKI94_01570"
                     /inference="COORDINATES: protein motif:HMM:NF017257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HNW25459.1"
                     /translation="MRIFLTLSVIFFLLSATPCEALFSKKDYKQIFIQSAQRAEKRKN
                     DKAAFNLFEKTMYYYKDDKQVLEEYAGFCERRGYNEKAVELYTKLYALYKDEYYLYRK
                     YFNEAKSGKLDKNKFSKIQKDKHLKPSQTEKINLELIKQFSYNNDWVGAKKACDKVSP
                     KNLNLETVVICTVSSEKVSDEKAAYKYYLRYYEVDPKNTSIIKRIIKLSDSFGDYKIE
                     EKFLKIFSSQNPNDNGIKYSLAGFYEKQGNFSRAAKVYEALMASGDRSEHVKKSHAYA
                     LAELNPKKHQKGFQKRVLTEREIKENQLYEALRAKNYKKTLDITEDLLKKYPDDKKLL
                     ILALENSMAQNLWDKTTIYNEKLLLTEPDSEKFLKNRAAIFSIKKDFKNAALSYEKLI
                     EKYPKPEYKFELANMYMANKEFEKAQEVIEPLYKENPENPDAVKALLNSLMAQNKTRN
                     AYIIIKTHHLENTPQGYQILGDMAMLNDDYESASRHYYRALQADKDNLLMKNKLAETY
                     RLLGYSEASQRIYEDILAQEPENQMAQIGLGYIQIDKKEFETARNIFNNILAKDPDYT
                     PAKEGIVFSYIGNGDDFNTLGALNSLPQNDETKLLRAQTYYKMGMPDNAKAALQGSVT
                     KEARDLRYKIRRDKAITVTPTYSFFFQQLAEQFKLDYHKFGVNVSQAAEGNKTVFAEY
                     NVIVYSSGGLNQQNNVVNEFKGGVYARPTKELEYRADLGVKAFQFGNGAMIITDSWIK
                     RYFNDNFAVKLGVKRNNVEQSYLSAVGTPIDGIFTGRAADNKVYLDLQIKLPKQCYAF
                     LLGSYGAIYAQNLITNMYYEGMVGLGRLWYNNPKNPWLQRFSTDIISYNSSYQYNLLD
                     LYSSTGQLYGGYFSPSYFNATTLNLKLEGEIKKWHLKYGIKGFGGIQEAITPDFTRLT
                     WGVSPYATYRINDHIDIVAEYNHFTYANLLRDQFMISAVIKGFKNAKN"
     gene            complement(11331..11576)
                     /locus_tag="PKI94_01575"
     CDS             complement(11331..11576)
                     /locus_tag="PKI94_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25460.1"
                     /translation="MAVNVAKFSDFAEEEFFPQNKGEISDSITQNWTEQALECYRLNC
                     DCSKCSISKGQYSFACQMPKVLELLVSIAGKPSVAEI"
     gene            complement(11755..12336)
                     /gene="yihA"
                     /locus_tag="PKI94_01580"
     CDS             complement(11755..12336)
                     /gene="yihA"
                     /locus_tag="PKI94_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNW25461.1"
                     /translation="MKQLSAKFIVSAEKLSQCPDFKLAEFPLLGRSNVGKSSFINALA
                     NNNKVAKTSNTPGKTRLINFFNFSDKFIIADLPGYGYAKVSKEAQDKWQKYLEEYILN
                     RPQIKCLIQFIDARHDIQKNDFQMRQWVLAHNLPVMTIVTKIDYIPKTKIQNVLNEVQ
                     KEFGGVVLPFSATDKRYCEEVLSTLLQEANETN"
     gene            complement(12361..12636)
                     /locus_tag="PKI94_01585"
     CDS             complement(12361..12636)
                     /locus_tag="PKI94_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25462.1"
                     /translation="MNIFKTFDIFLREPLSLFRDKIIQIVGINSGGSKRASVSAEFLD
                     KKTQITVYNSDKMYNLLRTVLYKKELQKNNQLQKNQNVLVGQLLKRY"
     gene            complement(12869..13333)
                     /locus_tag="PKI94_01590"
     CDS             complement(12869..13333)
                     /locus_tag="PKI94_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25463.1"
                     /translation="MKISTVNAFYQNQNNYSKHSNPSFGIHLGEHFKETIGFMSDRKS
                     LSKKSKELIKKIDNNHNFDDWILDINNSGKYLTLFPTVALPYKKYLHASPVAEIHGEL
                     MENALEEFLEASSAEEKITLAKKDIASQLKYIEEHGKQHIDTKSFLDTLNAI"
     gene            complement(13431..13946)
                     /locus_tag="PKI94_01595"
     CDS             complement(13431..13946)
                     /locus_tag="PKI94_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25464.1"
                     /translation="MNITPINNFSQQNKKNLNSTPSFGIGFHSSAKNFYKQVRPELPL
                     EQRKAVKEVLMHLRQNKILPDWIIGTAEDKQGFHITALKKGSEHLVDKPISIWHLTEP
                     SASASEKVHELLTCLRDDLDFFTRRLHTAFEELQSKFDFKKAREKALKEQQKLQDEFF
                     KKHPHLKDVIL"
     gene            14283..14834
                     /locus_tag="PKI94_01600"
     CDS             14283..14834
                     /locus_tag="PKI94_01600"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNW25465.1"
                     /translation="MSVEITGLDITLNRINTIERNFQALSGELNKPDSEFQSILDGYM
                     RINNGEDVSKAEINNLIDVYSAKNNLDADFVKAVVKQESGFNEKATSRCGAMGLMQLM
                     PGTAKSLGVENPYNAEDNIKGGTKMLSNLLKTYGGNKELALAAYNAGGGAVKKYGGIP
                     PYQETQNYVKSIMSMYQRTKGGA"
     gene            14835..15638
                     /locus_tag="PKI94_01605"
     CDS             14835..15638
                     /locus_tag="PKI94_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HNW25466.1"
                     /translation="MLLRGLASASNGMQALIDRNDSIANNIANVNTVGFKKENMVFKN
                     IYDSAVVEKSADTDEAKTLGELSIGSQVEKLTYDFSQGALSRTGGTFDLAIEGDGFFK
                     IKNNNGEVSYTRNGSFTLNNGGYLVTKDGDFVLDDQSQKIRIGLDGLKMHSLNDLIIG
                     EKGKIQVNNEKNQVNMQTVGVFDFSNKEDMTAIGGAKFKPTDIATNKELKAEKFTIQQ
                     GALEMSNSNVVQEMINSINTSRNYEALSKMVKMGSQTISNALQLGRIRG"
     gene            15659..16444
                     /gene="flgG"
                     /locus_tag="PKI94_01610"
     CDS             15659..16444
                     /gene="flgG"
                     /locus_tag="PKI94_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNW25467.1"
                     /translation="MLKALTTAATGMIAQQNYLDVISNNIANVNTTAFKKSRIEFQDL
                     LSQAVKTPGAMINQGTFQPVGIEMGLGVKTAATTRVFGQGVAISTGRNLDVAIEGEGF
                     FQIMKEDGTLAYSRDGAFKVDAMGQLCTTDGLLVQPQTSIPQDASEINITQDGNISVK
                     IGSNTTQQIVGQLQLVKFQNPSGLLSVGHNLYVETPGSGSPIQDTPGQNGMGLIQQGF
                     LEGSNVQIVDELISLIKAERAFESNSKIINSSSEILKQTNNLA"
     gene            16449..17156
                     /gene="flgA"
                     /locus_tag="PKI94_01615"
     CDS             16449..17156
                     /gene="flgA"
                     /locus_tag="PKI94_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNW25468.1"
                     /translation="MKKIINLALITILTLSALKTQARTFSSDEVKNEITQQVIESYKK
                     YTDADLKATVLAVPFKDMALPDGKVRFEVTSNVDKFMARDLKKVNIYVNDKFVRTFNA
                     PVDVKAYKDVLVAADFIAREKSLTRSMVQIKRMEVSYNLEYALTENMLGKDIITKKTF
                     KEGEIIDKRFVKLRPDVQRNSEITAFFKRDNILISVDAIALSDGMTGDYIGVENKSYK
                     KIYTGKIIGENKVLIEM"
     gene            17271..17894
                     /locus_tag="PKI94_01620"
     CDS             17271..17894
                     /locus_tag="PKI94_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNW25469.1"
                     /translation="MKLFKKNLIVLAVLAATVSYVSAESLFTLGASQTYSPAPKSLYG
                     GVQARQVGDLISIIMSESIKISDSLTYSASRESTTKDNFSNLINSFIGKDVINSNINN
                     FGGSNEVDSSASNSRNMSFGDMIAVQVVQQMPNGNLVVQGKKTIVNGNERMDLLVSGV
                     VDPRWINAAGQVYSKNVANLQFASSGRGSISRSGNEGIINRVIRYLF"
     gene            17933..19042
                     /locus_tag="PKI94_01625"
     CDS             17933..19042
                     /locus_tag="PKI94_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNW25470.1"
                     /translation="MITTIKKVLISALALLLTVMPASAINSTVRIMDITHVKGVRDNQ
                     VIGYGVVVGLPGTGDNSRSTQITNKMLLLNLGTVIEQENYIQKGSTAAVIVTATIPPF
                     AKNGDRIDVTVSTMADAKSLEGGVLVQTILKAPNGEAIAVAQGPLSVAGVNAKAGGSS
                     TRTTITTTGRIPGGAIMERDMVTDIGDADTVTLSLDNADYTLISRIAQTISSVIAPAK
                     ALDGTSVQVTVPEQFKDDRVGFLSILENLEVNPTMEKAKVIVNERTGTVVLGADVKLL
                     PAAVAHGNLTVTVTTTNEVSQPNAFAQGSTVGFSNSEIIVNKTPGSVIAIPANSNLND
                     LVRALNSIGVTPVDLIAILQALKKSGSLQAELIII"
     gene            19052..19387
                     /locus_tag="PKI94_01630"
     CDS             19052..19387
                     /locus_tag="PKI94_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25471.1"
                     /translation="MEINPLNYANMTETVSGEQQILNRIKDTPSSKEQLKKVASEFEG
                     IFISKMFSLMDETVDRENGIFGEETKHLDKFKSYMYNELGRQLASNPKTSFGFAKQIY
                     EQMERHLKD"
     gene            19400..19672
                     /locus_tag="PKI94_01635"
     CDS             19400..19672
                     /locus_tag="PKI94_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25472.1"
                     /translation="MVNSIGISGVNPIQYLNATNAFKSLQAEVPEQIIPEFSDGIGVN
                     QENLIAEQNMEEIKQIVQSIGEENISDDDIKYGVTYGRSVIADYVA"
     gene            19684..20184
                     /gene="flgN"
                     /locus_tag="PKI94_01640"
     CDS             19684..20184
                     /gene="flgN"
                     /locus_tag="PKI94_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNW25473.1"
                     /translation="MIDNTLTKFEEAIDEEIRAYENMEDLYKLKQAILIQGKSDVLWD
                     VDAKIVDNMKNIRNLNSKRKEVAKYLGDENFTISDVIDKAKLSNDTLAKKLTEQKVKL
                     NVLSSSLSLYEKTNLDLIKHGLVMVGKKLNIIVNTVLPPPNQYNSNGKTIGNEKIEIS
                     SIVEEA"
     gene            20210..21745
                     /gene="flgK"
                     /locus_tag="PKI94_01645"
     CDS             20210..21745
                     /gene="flgK"
                     /locus_tag="PKI94_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNW25474.1"
                     /translation="MSLTSSMNIAQQALLVNEAALNVVSNNISNVNTIGYSRQRVCLS
                     ANVNHTSFGGSAYLQAYSGTGVNIDRVERYTDAYLQSYYRQQNSLYNYLNQAANIASN
                     VEDIMNELNGTGLQAAFTSFFEAAQTLNLNPSDISARQNYAQQVQTVAMKFNELGSSL
                     KDARTALVGDVNEFGSLQSSKIYSLTTLANEKLQEIAKANADIVKISSPGMLPSGLLD
                     TRDQLIEELSQLVPVSIVENSNGSVNLSINGIEMIKGSQQTGTLDVVAGSQSEPAIIR
                     VKDLQGEVVYSNINSYLNSGSIGAVIDMASEEGDVLTIKSTMKNLDKLAKGFSDIINR
                     IQTQVIDGKTSLAIDKDTMLLTDATENIFESSDGEEINALNIRVNSDILEDPYLIAAA
                     RLDKTDPNYSAGAIGNNSNMLMVLDVRTNGSSELDNFSPESFLSQMVGDIGLKTASIN
                     SSLKSQSAVLSQVSSQLSSATGVNINEEVIDLTKYQAAYQASARVYSVCNDLLDVLLN
                     LGR"
     gene            21759..22691
                     /gene="flgL"
                     /locus_tag="PKI94_01650"
     CDS             21759..22691
                     /gene="flgL"
                     /locus_tag="PKI94_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNW25475.1"
                     /translation="MILRTTNQATNNANLNYIFSNQIKYNEISQQIASQKKILVPSDN
                     AIDSMNILNINRQMSKLNGYLNNMSMAQNELSVLDNSLASITSSLQKANDLAVQAASE
                     TNGTNNLQSMKVEIDQIIENVISLGNTKYNGQYIFAGTNTGTTPFQAVSTGGVEYTGT
                     PQDGPYQRNIEISDGVLAPINVPGDRLLGSYDAATETGTGVLKTLYQLSDAMGAVPPD
                     FDTIRASLDEIQGGITSTTNIRTNFAAMTARFKMTETSVNNSLLQLTSYKSQMQDLDL
                     AAAATNLANTELALNASMAVASNVIQQLSLLDYM"
     gene            22848..23771
                     /locus_tag="PKI94_01655"
     CDS             22848..23771
                     /locus_tag="PKI94_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNW25476.1"
                     /translation="MDKKIELDLDAVVAECRLTEDTEEDILIKNIDNLESIYDGKNLV
                     AIYNKFLTVAKSPNILMYLIRCVDKYRDKSSLSSLLDLLFDKKNSDGVRVMCAKVIAN
                     LKDTSAVTSLLYCLNDKEENYKVRLACADALGRIGDKYAVAPLINVVQDEDEKSVYVR
                     ESAAVALGMLGDARAVDPLVSILETKKGFVDKFTFLKERIIEALGKLNLSSDRVFNAL
                     EHALCDESHLIRINAIEALMESEDERAYGLIKKMLEDLHEEVRKNALIALYNMVGRDI
                     LDEIISGEYDQSLKIEAQAMIEEYETGEEEP"
     gene            23768..24448
                     /gene="queC"
                     /locus_tag="PKI94_01660"
     CDS             23768..24448
                     /gene="queC"
                     /locus_tag="PKI94_01660"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNW25477.1"
                     /translation="MIKSIILLSGGLDSLVSLAVSKEKFNIELALTFDYGQKSAKKEI
                     EASEKICKYYNINQKIINLDWLKEITQTSLVGDKQIPKVSRQDLASDDFVQNSAKSVW
                     VPNRNGLFLNIAGCFADSFGYDYIIFGANKEEGQTFPDNTEEFAEIISQELQYSTLNH
                     PKILAPLINSNKDDIVKLAIKHAVPLNLTRSCYSDKQKHCGQCESCIRLKRALEANNC
                     QNLIKELF"
     gene            24451..25020
                     /locus_tag="PKI94_01665"
     CDS             24451..25020
                     /locus_tag="PKI94_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HNW25478.1"
                     /translation="MHTKFIEKEIKYTGAELCPHWIYRTFKLQGDAVVAFCGECEVKL
                     TEMVDIEDVINNEPIYSKSMLNFIVEHFNTGLIEGVTRQRLLICIIKEELENLFTIYH
                     LPFTLQRRGDDLFIDNKKLSVSIATKSSTSVLMHIGVNIDPEGAPIEAIGLKDLGISD
                     INDFAQKIMVKYSKEIDDIILASTKVVGR"
     gene            25035..25718
                     /locus_tag="PKI94_01670"
     CDS             25035..25718
                     /locus_tag="PKI94_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNW25479.1"
                     /translation="MKTPDYLSYTSYKKKKQNSSKKSMVIFVTTFFATLLVFTAIAKQ
                     LTPNVDVTIGEDTDMEAKETGLGVKRFIDDRLKMIQMEDNSAGVSKRDSDKSIASAED
                     ENFDSFAEDLDERVYLPKKKPAKEEVAEKPAKETVTPPRPKPNDEINPFDAPKMSKVF
                     VGYYSSVEQAKVAQGILMDADLGVTPFIKNMGGAFTLQVGSYSSKIKALELANELLRN
                     NFPARVVQE"
     gene            complement(25801..26571)
                     /locus_tag="PKI94_01675"
     CDS             complement(25801..26571)
                     /locus_tag="PKI94_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25480.1"
                     /translation="MAIKVCQTKKNISDFCTNPILDKPALAYELCVKADALRLASLYQ
                     EAVSKYLNAILIDRNNPESYYGLGVCYKHLNNLDKAICALEKAKKLKEFDSNIYYELG
                     ICYLLSNQSCCAIKNFVKSLKLNPNNLNAQIQLAIAHEIVEENDMALMIYQKIIETNP
                     GYLKAYNHKSALLMSMGDYTQASLIFHKTLKLNPNYNKAYLGIGICFDKMGKNADAQR
                     YYKKFLIQKPHSQHASFVKSRLEHLKTKAGNKNFLSIV"
     gene            26729..27607
                     /locus_tag="PKI94_01680"
     CDS             26729..27607
                     /locus_tag="PKI94_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNW25481.1"
                     /translation="MKRFLILFVIFIFQMIFILPCHAIKIGLQTGMDRVYIGSSTQAQ
                     IINGRTNRLLYTINGMKAYEVRAYRSGIAIKVLGKYFNLDSDYVVVKPSQGGFISVKN
                     KWYRGDFIICNRNGKLNVVNDVGIEDYIKGVVPSEMPSKWAYEAHKAQAIAARSYALA
                     NLGKRAAGGYDLKDTPEDQAYGGASAESEQTNKAVSETKGIVLIYDLKIIPAYYSASA
                     GGQTSTSSEVWTKNLPYLQSVPSFDDNIRKNGHGVGMSQHGANNLAKSGYNAYQILQY
                     FYKGVQFARINPEYYK"
     gene            27913..28185
                     /locus_tag="PKI94_01685"
     CDS             27913..28185
                     /locus_tag="PKI94_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNW25482.1"
                     /translation="MNKEELVKEVSKKAKVSQKAAADVIAAALDTIQKTVSKGKKVTL
                     VGFGTWEPRKRAARMGRNPQTGAQLKIAAKVVPAFSAGKKFKELVK"
     gene            28317..29009
                     /locus_tag="PKI94_01690"
     CDS             28317..29009
                     /locus_tag="PKI94_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25483.1"
                     /translation="MKVLFNNKYKTYNLNLKNSDTRNRSFLIQKPDTVSFGMANIGDD
                     GDFELNQIRVNQKRFETNIRQHIVSIPDTLLFLQDHNGVTQFMRALYDEHPNNAMRIL
                     DYVKDANFDLKEKKKFFELKDKDGNSQFDVAIKTKYYEIAMKLLKLMAKINPEKVVDL
                     NIPEEIYKMISFPRVVDIMIENEALSKEQTLKFLDKNMEYVPLYEKRRALVEDYADIY
                     ITDEDISGANWV"
     gene            complement(29348..29422)
                     /locus_tag="PKI94_01695"
     tRNA            complement(29348..29422)
                     /locus_tag="PKI94_01695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29388..29390),aa:Gly,seq:gcc)
     gene            29560..30933
                     /gene="dnaB"
                     /locus_tag="PKI94_01700"
     CDS             29560..30933
                     /gene="dnaB"
                     /locus_tag="PKI94_01700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNW25484.1"
                     /translation="MAVNTAKKIDDNLIPQNIEAEEAVLGAILVNPNTITKVVEFLKP
                     ESFYKPAHRYVYEAMLQLFNQNERIDLVSVSDVLNFNAKLEVIGGRAFINDLSYKTIT
                     TSNIEYYAKIVQEKAIKRSLINAGSEIITFGYDLNPIDESLDNAEKLIFDIAAKKTTK
                     DLLHVKDLVLQSYEKIEYRYNHREEMTGVPTGFYELDNITSGLQKSDLIILAARPSMG
                     KTALALNIAQNVAIRAKFPVAVFSLEMSKEQLVQRMLCSEAEVDSQRLRTGHMQSKDW
                     EKLANAMNDFAQAPIYIDDSPGCSLTDIRAKCRRLAMQEKNLGLIVIDYLQLMEGSGK
                     EERIQQISAISRGLKTLARELDVPVIALSQLSRAVEQRKDRRPMLSDLRESGAIEQDA
                     DIVMFIYREDYYNREEGDEMPKATGKEGKSDILVAKHRNGPVGNFELIFQSNITKFKN
                     PIKTDVF"
     gene            complement(30957..31808)
                     /gene="pheA"
                     /locus_tag="PKI94_01705"
     CDS             complement(30957..31808)
                     /gene="pheA"
                     /locus_tag="PKI94_01705"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNW25485.1"
                     /translation="MTFLERVESLVYLGPGGSFTEMAKDIFCQKYNLDPYLVPMKSIK
                     KAVEYVDENPYSLAVLPIENSIEGTIRETIDSLIKTQNPNIKVVAELIMPIKHCLLSK
                     TTELYSITGVIAHPQAISQCQNFIHDKLPLHTNIIEVANTAEAAKSLQNYNLTYAAIG
                     TQKTADIYYLNVLLDNINDEQDNKTRFALIGNYNTPKTGKDETSIAFSTENKPGALLD
                     ILKIFQDFEINLSYIDSRPSKTNFGEYTFFVDFDGHILDDKIAEALAQIQKHTPYFRL
                     VGSYEKF"
     gene            31867..33261
                     /locus_tag="PKI94_01710"
     CDS             31867..33261
                     /locus_tag="PKI94_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25486.1"
                     /translation="MANIKFGTDGWRAVVGEGFNSQNVEIVTNAIGKYVFDNFGIDKK
                     ILIGHDSRNMAFEFANQCANLLASKGFEILMSKKVLPTPILAYNAKHKNACAIMFTAS
                     HNPPQYLGMKFIPDYAGPATDEITDEIVRNIKSNQTIQLFDCSTVQLTDFMPDYFEHL
                     KELIDFESIKTLKTNIIFDGLYSATIGYFDNILDEFEIKYDSIHMFHDKNFGGAMPEP
                     KPKYLKELIEKVKSNPNSIGLANDGDGDRFGVINENGEWVSPNEIIAILLLYLKNYKH
                     LKGPVVKTVGASLLFDRIASKIGVEVIETAVGFKFVGAAMREFNPIIAGEESGGLSIQ
                     GHIPEKDGILANLLILEAMAVIGKSLVELQRELWFLVGTKFINDRVDLHLQNDEQIKA
                     ALEKVSAIKNIGKFKVIKTCTKDGIKLYFDDNSSWVLVRASGTEPLLRIYFESDNHDK
                     LEFLKESLKKTLSA"
     gene            33310..34308
                     /locus_tag="PKI94_01715"
     CDS             33310..34308
                     /locus_tag="PKI94_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25487.1"
                     /translation="MVSVKLQTFAKYLDQPALVKHLDKKMPVVLSALGLAYGINDSFQ
                     APKDEQYKTFIQNLSVLSLTIVSALAATRGLKLFGKKIEGLIELPHLHKKDISEVLKK
                     ITDDKTKKLIQKVLDDKILKFQEVKNLKENLKKLYDININKIIPDAHNHGAFEELGKL
                     SLLGLIPVLGGITGGVIGDVLTKDNWKKKFPNKIKEGTYQYLNNIFLCNVGAGIALAT
                     MNRLKIQSKIARFGAMLAGVVGVGLIAGSAIANFIGKNFINPIFDKKEIPKRDFHSMT
                     ANLNSERHPEALDLSLHIDDIASVGFLSGLKWIGPVLPLLYSVSAYRAGIGYRNSK"
     gene            34406..34789
                     /locus_tag="PKI94_01720"
     CDS             34406..34789
                     /locus_tag="PKI94_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNW25488.1"
                     /translation="MSEYINNIRFDAKKAENFFAQRLAYTLGPVELSEMMKEGKVKLI
                     DVRYPDDFVEGHIPFAVSMPKIELAQRLSELSKKELHVVYCYNQQCHLASSAALILAK
                     NGYPVMELEGGFDVWKNGFKFDVEK"
     gene            complement(35168..35764)
                     /locus_tag="PKI94_01725"
     CDS             complement(35168..35764)
                     /locus_tag="PKI94_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNW25489.1"
                     /translation="MIEKYEKYLEIVGSYLDKYFKQQKPYIHCKEGCSLCCKCGEYPF
                     TRLEMQYLMSGFMKLDEASKKTIREKISELKKEKQKTLENGEKFMYECPFLIDKRCSV
                     YDYRGLICRNHGLMTMFTNKDKKETFQIPYCAREDLNYSIVYDKKRGTISKKLWRKSG
                     IKQEPLSYNVGLDYLLNNEFTQNLNINFGEQLALIDWF"
     gene            35878..37659
                     /gene="thrS"
                     /locus_tag="PKI94_01730"
     CDS             35878..37659
                     /gene="thrS"
                     /locus_tag="PKI94_01730"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNW25490.1"
                     /translation="MEKNNETLHVLRHSTSHIMAQAVQALFPGARLAIGPSTDTGFYY
                     DFDLTDGHAFTEDDLIKIEEKMKEIIKQNLTFEKYFVEDVDAKIAEFKKQGEIYKAEL
                     LEEHRNDNPTLYLTKNKDGKVLFNDLCAGPHIPYSSFIKPNAFKLLKVAGAYWRGSEK
                     NKMLQRIYATVFWTKEDLADYLNFLEEAEKRDHRKLGTALDLFSTREEVGGGLVLWHP
                     NLAVVREEVENYWRNEHRKRGYVIVNTPHIAKSKLWDISGHTGHYRDNMFFIHEDDEE
                     YVLKPMNCPFHIMIYQSNRYSYRSLPLRMAELGTVYRKEKSGALSGLTRVQGFTQDDA
                     HVFCTPEQLVEEINKIIDFVADTMAIFNMEFDVELSTRPEENYVGTIQNWDLAEAGLK
                     EAMDKRKMKYEINEGDGAFYGPKIDFKVKDAIGRTWQCATIQLDFNLPERFELKYQDK
                     DGQLKTPVMLHRVIFGSMERFHGLLIEHYAGAFPTWLAPTQVCLVPISNEKHADFAQK
                     IYEKLNNEGIRVKLDDRSESMNYKIREALQDKKIPYVAVIGDKEIEANSLAIRARGKG
                     QIGVFEIDDFVKKLSEEIKSKGKKIVE"
     gene            complement(37728..37916)
                     /locus_tag="PKI94_01735"
     CDS             complement(37728..37916)
                     /locus_tag="PKI94_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25491.1"
                     /translation="MDNKITIRSDRKNDYTFQYKNEDVVLKAGSIISIANGLNEVVFP
                     TCAMKIINNLIIIKDDVK"
     gene            complement(37931..38164)
                     /gene="rpsR"
                     /locus_tag="PKI94_01740"
     CDS             complement(37931..38164)
                     /gene="rpsR"
                     /locus_tag="PKI94_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNW25492.1"
                     /translation="MARENLKDLKKHKNCSFCADKIDFIDYKDASKLKRYLNEAGKII
                     PRRITGNCAEHQRMVAKAVKRAREASIISYVFD"
     gene            complement(38278..38670)
                     /locus_tag="PKI94_01745"
     CDS             complement(38278..38670)
                     /locus_tag="PKI94_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNW25493.1"
                     /translation="MTIAKAVVTGTVYRTPEKRFTTNNVAIWAFVLDIGEKEETLIRV
                     MCKRMSLDEVASSINKGDRILVEGRLEVATAKMDDGSERKIYEIDANTVEKMGATAPV
                     KNSNNEEIVKFAQEEFSDELIGEDEIPF"
     gene            complement(38763..39062)
                     /gene="rpsF"
                     /locus_tag="PKI94_01750"
     CDS             complement(38763..39062)
                     /gene="rpsF"
                     /locus_tag="PKI94_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNW25494.1"
                     /translation="MKNYELLTVIKPNLDLDEVDKVIAKIQETIESFGGKVSSTDKIG
                     RKKLAYDIQKFRDGFFTTQVLSIPADKVEEFKRQLKLNENVLRIMFVEMAKTQVT"
     gene            complement(39121..39942)
                     /gene="dapF"
                     /locus_tag="PKI94_01755"
     CDS             complement(39121..39942)
                     /gene="dapF"
                     /locus_tag="PKI94_01755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNW25495.1"
                     /translation="MNKINVTKMQGLGNDFVIVDYCEYEKSGLPMDELAKKLCDRNFG
                     IGADGMIIPALCTKDADIGWFFYNSDGSTAQMCGNGIRCFAKYVYDRKLVNSKKFSVS
                     TLAGIIIPEILEDGQVKVNMSKPRGECTKNNLKIKGRIFETNIVSMGNPHCVIFVDTD
                     EDLLELAKTYGPEIEKHELFPEKTNVEFAKIISKNEIDLRVFERGCGITLACGTGACA
                     TVVAAVLNNLTEGKVKVNLLGGALTIEWQGFSDNTDFDVFMSGPAQYSFLAEYLL"
     gene            complement(39970..40767)
                     /locus_tag="PKI94_01760"
     CDS             complement(39970..40767)
                     /locus_tag="PKI94_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25496.1"
                     /translation="MGIINGVLKIGSTGLRKALPAVSKKAPILSEVSADLISIGNKTS
                     PQPVIKNFNLKSVLNFVRNIFSKNKPPKIVKISDDLFKKTFGNLEAFFSKMGCAQDLV
                     NFKKPPKLLKGLKEITEIKQKNHIHNIPEGLVESFIQELEKSGLYKGRKIKGIIGVGS
                     KTAAFELDNQEVLKIALSNNLTTRPHNSLFDLPVLDSGKIGDVYYYIQPKADRTNLTT
                     EHVIDVINKIKFSGYTPRDLQTNRFDQIGLYKGKPYLIDRECARQAV"
     gene            40924..41011
                     /locus_tag="PKI94_01765"
     tRNA            40924..41011
                     /locus_tag="PKI94_01765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40958..40960,aa:Ser,seq:tga)
     gene            complement(41297..42091)
                     /gene="arsM"
                     /locus_tag="PKI94_01770"
     CDS             complement(41297..42091)
                     /gene="arsM"
                     /locus_tag="PKI94_01770"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNW25497.1"
                     /translation="MEFDIREKVKEFYGDIASDVSKGNTSSCACCDEISKTSVIYDGA
                     ELDDLPIEAINASLGCSNPLVLAELKEGETVLDLGSGGGIDVLMSSKYVGKSGKIYGL
                     DMTDQMLELANKNKEKMGADNVEFIKGYIENIPLADNSVDAVISNCVINLSSDKKKAL
                     SEAYRVIKEGGRLAVADVVSLREVTPELKKQAELWAGCIAGTILINEYKQILSDVGFK
                     NIEIEPMHIYSKAIIEELVKDKNELAKINLEEIDGAFAGAHIKAWK"
     gene            complement(42184..42477)
                     /locus_tag="PKI94_01775"
     CDS             complement(42184..42477)
                     /locus_tag="PKI94_01775"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNW25498.1"
                     /translation="MNKELYKKKTKMIKALANPTRLLIVDLLASGEKNVSEIIELTGE
                     EQSNVSKCLAVLKSNGIVKDRKDGMNVYYSLKICCMGEFFVCMNKMFEENSGN"
     gene            42562..42792
                     /locus_tag="PKI94_01780"
     CDS             42562..42792
                     /locus_tag="PKI94_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNW25499.1"
                     /translation="MKKIEILGGGCQKCNDLAEKVKAVADSMGIEYELEKVTDITKII
                     NYGVIKTPALVVDGEVKVSGEIPSVDEIKELL"
     gene            42789..43169
                     /locus_tag="PKI94_01785"
     CDS             42789..43169
                     /locus_tag="PKI94_01785"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNW25500.1"
                     /translation="MKKLLLILTILLGFSNTVFAADKVNVYYFYGKPRCVTCQKIENY
                     TKQAVESMKDKDVNYKSIDMEKSENSSMVKKYNLFTKSVVISKTKNGKEQWKNLDKVW
                     LKVGNEQDFKNYIITETKIFKGSK"
     gene            43169..43840
                     /locus_tag="PKI94_01790"
     CDS             43169..43840
                     /locus_tag="PKI94_01790"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNW25501.1"
                     /translation="MVIAILSALWLGILTAISPCPLATNIAAISFISKNIECPYRTVW
                     AGFLYTLGRVLAYVILGFIVVGGLLAVPSVANFLQHSITKFIGPLLILIGLLLLNVIK
                     LNFSFGVKHEKIQEFAATSNFLGAFLLGVIFALSFCPVSAALFFGSLTSLAVEYHSRL
                     IIPTMYGIGTGLPVVIFAIVVAFSIHKLGEIYNNMVKFEFWFRRITGGIFIIAGVYFI
                     VIHFM"
     gene            43858..44244
                     /locus_tag="PKI94_01795"
     CDS             43858..44244
                     /locus_tag="PKI94_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25502.1"
                     /translation="MTEELPGFIMCICTGKCPGFQAMDIWDFINQVRVELPVEYAFIH
                     PQLCEEDGDRFLADFLKANRKVVIAACAPNMQRKMFKDAFKEAGLDLEKDAVMLDIRD
                     MTNQQAMKKVAKALSDMGLDADEEDY"
     gene            44244..44573
                     /locus_tag="PKI94_01800"
     CDS             44244..44573
                     /locus_tag="PKI94_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW25503.1"
                     /translation="MSNNDEDMKILPPTGDTFMGVPRDKIDWCPTIDPKKCNDCMECL
                     KFCPHKVFDVDENAKPKLIVKNPKNCVVFCRACSKTCGPDAISFPSKPETIKKIKQTR
                     KGLNQIE"
     gene            44777..45637
                     /locus_tag="PKI94_01805"
     CDS             44777..45637
                     /locus_tag="PKI94_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNW25504.1"
                     /translation="MNKKYAILPCNGLDKSAGCVAREIALQIAEQTGSEIICPVLYQV
                     ADARYKKITQDNPLLVIDGCQTRCASKLAADKGLKVTKKITVTEYAKNNGIELCEPLR
                     IDDAAINLAKTLTKDALKETSSAENKGETFAMPEDIQYSTYQKDKFIFKTPTEGFLFN
                     ENDCWAYVIGNVARVGVADYVQHSLSDIIFFTPPNVGNEVEQFEELGQIESGKAVFEV
                     ISPVSGKIIRVNEALINAPELINQDPYVKGWIADIELSNFEEDKEFLLDYEGYFSVLK
                     RKVDEYHVKK"
     gene            45624..46016
                     /locus_tag="PKI94_01810"
     CDS             45624..46016
                     /locus_tag="PKI94_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNW25505.1"
                     /translation="MSKNKVKIIPCSGMGKAFGLIARESTLESIKKCPQDAETVCLAY
                     VVTGDEEAKKLVEGQKCITIDGCPAMCSAKNIEIAGGIIEEKIRVVDVFRNHRGVNAG
                     SATELTEDGWKIVDEIAEDVAKKIKGAK"
     gene            46016..46423
                     /locus_tag="PKI94_01815"
     CDS             46016..46423
                     /locus_tag="PKI94_01815"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNW25506.1"
                     /translation="MNKNNTKIGVVSCSGEDCLGGTISRLATRKMLELRADETVTICL
                     PLYLVGGEEERGFANEYPTISVDGCDKYCGKRATEKYSGKVSASINVGDIIGEETAKS
                     AALSCRDLTDVHKAMVKKVSDVIQEKYDEILKK"
     gene            46492..47232
                     /locus_tag="PKI94_01820"
     CDS             46492..47232
                     /locus_tag="PKI94_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNW25507.1"
                     /translation="MFFIQNENTNPYFNLALEEYLLKEFDQDCFMLWQNEPCVVVGKN
                     QNVLNEINEDFIRKNNIKVARRISGGGAVYHDLGNLNFTFIINDTRNDLRNYRKFTTL
                     IIEVLAKLGIKAELSERNDLTIEGLKFSGNAQYKNKNRLLHHGTILFSSNLDNVKTAL
                     KVGKNKSGRWVKSFASPITNIQDHIRTHLSINELKTFIQNHFEGNQKNYHIYNLTLTD
                     IEKVNKLVKQKYATDKWIFKDSLIELYS"
     gene            complement(47505..48239)
                     /locus_tag="PKI94_01825"
     CDS             complement(47505..48239)
                     /locus_tag="PKI94_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNW25508.1"
                     /translation="MKQYSEIYLKAKVKLALNHYKRNKEQVLLIAPGWTMSKDSKFIK
                     QAAIEFAKTFDVISFDFRGHGRSSGFYTFSKKEPDDLNAVVDYAKGIYKKVYVIGFSM
                     GGAISIIQSAAQSNADCLIIVSAPHSFEKIKNVKWIKDFLTNPFKKYEFKKWIRVRVS
                     PIIQKMVKPIDVVDKINCPTLFIAGDNDTIILPDDTKSLFEKAKCKKEFELFEGCNHA
                     EDLIYQQKEKFIKTCTNWLCSCLRTA"
     gene            complement(48333..50012)
                     /locus_tag="PKI94_01830"
     CDS             complement(48333..50012)
                     /locus_tag="PKI94_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNW25509.1"
                     /translation="MKYKKSNILLLLEEKTDVYNDRVALGIKTALGWKEFTFKGIGLL
                     SRKLASYLINDIQVKKGEKLAILSESKPEYGACVFASVMSGMVTVPLDVKLTKYELKS
                     ILLDCQPTILLTSQENIDKALDLQKEIKSIKHVILIDEPSYNLQMPCLYTLPNNYDCK
                     WRHRSSKSTAFIIYTSGTTGAPKGVEITFRNMLAQLNDMSKVYYHFFDKNEGISVLSI
                     LPMNHLFELTVGFSTFLYWGLSVYYTQSLKPKDILSIMREKGINFMIVVPAFLKLLKA
                     GIETEIANSPKSVQVGFKVMYNIAKRIPSYAVRKMMFKKIHDKFGGNFNGCIAGGAPL
                     DLAVAEFFETIGIKVYQGYGLSETSPVVSVNYDKRWMLASVGRPLESFEAKIDPKTGE
                     ILLRGPSVMKGYHNQPELTAEVIDADGWLHTGDIGRIDEDSHIFITGRLKNMIVLSGG
                     KKVFPEEVESVLEKSQYFAEVCVLGSARSGGSKDGTEDIVAVVVPKEDLINKVKDEKE
                     INRIVREEVKYLSNQLASYKRPINIIVHNEPLPKTTTRKVKRKDVKELLKI"
     gene            50268..51575
                     /locus_tag="PKI94_01835"
     CDS             50268..51575
                     /locus_tag="PKI94_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25510.1"
                     /translation="MGMAASQARYLGLTARKTNTEYEGQQINQQRTALANQSAGLFNR
                     MLSLEVPTPPSTQDYTKVSYTYSDGVNKETISSFTPLAGDPDYNYTVTHFHNSQVYTG
                     IETKQVNPQVRKSGSTYTYIGNSPLTLVSVAKQQAGVTEPQIIYSGDGATRTYTSLTE
                     GGSSRSLSAYTTADRAALEQLAALHPTSNLGQTFLDGDTSEIYRYTGSDGEVHYVCKD
                     DLDASIGSTASGGAGMAALDNYTGGDTTSWAAVNQIVTDNASSQFAEDAIAGDDIYSY
                     QLGGVTYYVCESDLDASYNSIIPNGAFEGQSALNRYNSAYLDTRVETTEKAFLQQDQS
                     GRFISAKLENSSPTFTLGSSTVTDDNAYNDAMNQYNYNTFKYQKEIEDINAKTKIIQE
                     EDRTLELRLKQLDTEQKALQTEMEAVKKVIDKNVEMTFKTFSS"
     gene            51775..53079
                     /locus_tag="PKI94_01840"
     CDS             51775..53079
                     /locus_tag="PKI94_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25511.1"
                     /translation="MGMAASQARYLGLTARKTNTEYEGQQINQQRTALANQSAGLFNQ
                     MLSLQVPTPPSTQDYTTVSYTYSDGVNNQTISNFSPLAGDPDYNYTVTHFYNSNIYTG
                     IETKQVNPQVRKSGSTYTYVGNSPLTLVSVAKQQADVTDPKIVYSGLVGVRTYTSLTD
                     GTTSCTLSAYSSTADRAALEQLAALHPTSNLATAFLSGDTDEIYSYTGEDGKVHYVCK
                     TDLDTSIGGISSGGAGMADLDNYTGGDATNWAAINQVITDNASSQFKTDAVAGDDIYS
                     YQLGGVTYYVCESDLDASYNSSGGAIEGQIALNRYNSAYLDTKVETTERAFLQQDQSG
                     RFISAKLENSSPTFSLNSATITDENAYNDAMNQYNYDTMKYQQQVQDINAKTQIIQEQ
                     DRTLELRLKQLDTEQQALQTEMEAVKKVIDKNVETTFKTFGG"
     gene            53154..53993
                     /locus_tag="PKI94_01845"
     CDS             53154..53993
                     /locus_tag="PKI94_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25512.1"
                     /translation="MSYRYDSYLVIANKFSSAGSDAKAQLFETYDRLRDLTVSGSAAT
                     GTGFEQAGGFLYQVASLFSPSSFMTTMGGTSAMNIPGTSYWSPVSGGNAVVNGGYSAF
                     GIGGLGNYSGFPGGAANINLGLGDYTGGASSILSGFGSMQGSATGFASSIGGIADVAS
                     AAAYGAGMGSGYGFGTNVVLPIAGVISGWGGIMQAAAPYLGEYGLPAIVMGNLMQGTT
                     SAALSAYQNVSGRILSNADSILTNKVRNIETVCKMLDTQGDVVRKMLKESIDSDSKAI
                     QNL"
     gene            54317..54589
                     /locus_tag="PKI94_01850"
     CDS             54317..54589
                     /locus_tag="PKI94_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25513.1"
                     /translation="MYQETLQTNFKRFMNFLGFARSFFLRRNPIKTFINGLAEALKDF
                     LTMNKKLKLARYRLSYHRHQLNKMESLIAENNQHTFLRGNKINEVR"
     gene            54602..54937
                     /locus_tag="PKI94_01855"
     CDS             54602..54937
                     /locus_tag="PKI94_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25514.1"
                     /translation="MGLLIATLRKTYLIAYKNELEYKIQLITQAKIALTQSTNDLLNV
                     GTDMDPDNPVVKQLEQRKERLNLLEKKLDMQMDEYKTKLDMVEKEIQSCDGMFKSNLE
                     SSFSYGGGR"
     gene            55199..56533
                     /gene="tig"
                     /locus_tag="PKI94_01860"
     CDS             55199..56533
                     /gene="tig"
                     /locus_tag="PKI94_01860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNW25515.1"
                     /translation="MKVDLKKEKDNVVTLDIQVPAKDAENEYNKAVRKVSEYVNIPGF
                     RKGKAPRNIVEKHVGIERIQQEALERMLPAIFKDAITKNDLDIVSQPYVESYDFNLGE
                     DLKIVAKVELRPEIKLGEYKGLKLEAQGYTIPEDAFDKALEGLLQQHVKYELVVGRPA
                     QKTDIVVIDFDGSVNGEKIKGGAAENYPLDLANSNFIPGFAEQLIGKNLGEEFDIEVK
                     FPEEYHEAKLAGQPAIFKIKIKEIKEKVLPELTDEFAQKLGTFKTVEDLKADIQKYLE
                     ATKNNEDAKIISNTIFEKVLSGVKIDIQNSMVERETQSLLEEYKQRLMMQGIEWQKAV
                     ESQGEENILKGLKEEALLRIKNSLVIDEIAKVENIKIGPEELDKKLKEIEDAYKMPRA
                     ELMKQLSQNPQVFSSLSQQALNEKVAKFLNENNTVELAADKEKKAKSAKKND"
     gene            56595..57194
                     /gene="clpP"
                     /locus_tag="PKI94_01865"
     CDS             56595..57194
                     /gene="clpP"
                     /locus_tag="PKI94_01865"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNW25516.1"
                     /translation="MSFVPVVIEQSSRGERSFDIFSRLLRERIIFLGTPVDDMVANLI
                     VAQMLLLDSENPEKDIMLYINSPGGSVTAGFAIYDTMQHIRADVSTICLGQAASMGAF
                     LLAAGTKGKRMALPHSRVLIHQPLGGAQGQATDIEIQAAEIVRIKKSLNEILAANTGQ
                     SIKKIEKDTDRDYIMTPEEALEYGMIDKIVSKEILKESK"
     gene            57205..58518
                     /gene="clpX"
                     /locus_tag="PKI94_01870"
     CDS             57205..58518
                     /gene="clpX"
                     /locus_tag="PKI94_01870"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HNW25517.1"
                     /translation="MSKHDDSRLKCSFCGKTQDQVKKLIAGPEVYICDECVELCNEIL
                     DEEFFEGKDKDAAGEKGKEAEEKTIPKPAEIKSYLDEHIVGQEDAKKVLSVAVYNHYK
                     RLRHNKSKDKEGGVEIQKSNILLVGPTGSGKTLLAQTLAKMLDVPFAVADATTLTEAG
                     YVGDDVENILLRLLQNAEYDAAKAERGIIYIDEIDKIARKSENTSITRDVSGEGVQQA
                     LLKMIEGTVANVPPQGGRKHPHQEFIQIDTSNILFIVGGAFVGLEKIVERRADDGSSI
                     GFGAKTASSKAEKEAQAAKMLKKLQPEDLLKFGLIPEFIGRVPVYAVLDALDEKTLKM
                     ILTEPKNAILKQYKCLLGMDGVELEFEPEAIDLIAADAIKRKTGARALRSIVEEIMLD
                     IMYDIPSKEHMDKFVITADMVKNRNKAELIKLPTKKKLEQEEEIA"
     gene            59240..59503
                     /locus_tag="PKI94_01875"
     CDS             59240..59503
                     /locus_tag="PKI94_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25518.1"
                     /translation="MISSKVKVLVIISALLLSVGLMSVGCKSAAQKGWVNTDSMPGHT
                     FSKHKNKNNQNTQIVYLGASEKDSSTAVDFGPQDVSFEFSNLK"
     gene            59639..59980
                     /locus_tag="PKI94_01880"
     CDS             59639..59980
                     /locus_tag="PKI94_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNW25519.1"
                     /translation="MIGGVLEIEIMNCIWSLQKQDEDANISVADVVAFLSRNKVKRAY
                     TTIKTVMDRLSAKGLLARYKSGKKFFYRSTLDREEAAKDALKEVSTQFFGGNYVEMIK
                     FIEKECSAVLV"
     gene            59991..60353
                     /locus_tag="PKI94_01885"
     CDS             59991..60353
                     /locus_tag="PKI94_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25520.1"
                     /translation="MNIQDDRKSVSRLIYKVLTESLTVREAILNFPKDVNDASLKTAY
                     HALVHLEADEDLRRRDLLYRQEQDDYLEFIAQTLQKGEVLPYNIIKEYEKYYEGISTP
                     HSRSMKGILKSLCKFLNV"
BASE COUNT        19790 a        10193 c        11585 g        18809 t
ORIGIN      
        1 gaattgttga tttgtctgag gaattttcgc ttgtaacctg ttgcgatgga atcggctcaa
       61 aaattattcc gctttatgag aaaaaacttt ttaaaataat ctcggctgat gtcgtagctg
      121 caaatttgaa cgatttggtc tgcaccggtg ctaaagctgt cggttttgtg gattatatcg
      181 ccgcaaacaa actagacagt aatgaagttt tttcaatagt cgaagcgatt aaaaaagagt
      241 tggacaattg cggatgcaaa tttttgggcg gtgaaacctc agaaataaat gagttgatta
      301 caaaagggaa tatcgatatc tcaggctttg ctgtcggtgt ggtgaaaaaa gaagaaaaac
      361 ttgacaaaaa taacgttaaa gaaaacgatg ttgtggtagg gctttgttcc tcgggaatcc
      421 acgcaaacgg gttttcgctt gtgcgaaaac tgtatgctca aaaaaaactt accgataaag
      481 agtttgaaac ctgccttgca ccaacctgta attattacaa ctgcgtttca aaactcgttg
      541 agaaaaaatt ggtaaaatct tgcgccaaca taacaggggg cggaattttt tcaaatttaa
      601 tccgcgcaat tccagataat ttgtcggcag aacttgattt tgaaaaaatt ccgccccaat
      661 caatttttca aaagctgaaa tcaatctgcg gggatgaatt ttttgaagtc tttaacgcgg
      721 gtgtgggttt ttgcatgatt gcagatgaga aaaatttgga gcaaattttt gaaatttcaa
      781 aagaatttaa cccttttatt ttagggaaaa taattataaa aaataaaata ataacgcaat
      841 aacataatgt catgctgaat ttatttcagc atctttccag ctttgtatgt tggatagatc
      901 ccgaaataag ttcgggatga caaaaaagaa aaagaaggaa taaatgacaa aaaaattagc
      961 aataatgggt tcaggaaacg gcagtaattt tgaagcaatc gcaaaatttt tgcagggtaa
     1021 agatgtggaa attacttgtt tgagcgatgt taaagatgct tttattctaa aacgagctca
     1081 aaatttgggc ataagccatg aatttttgtc ctttgagaaa aattatgaat acttttcaaa
     1141 aaacaaattt gaccttgtgg cacttgcagg ttacatgaaa attcttgatg aaaaaacttt
     1201 gaaaaaaatg ggaaatgtca tcaatattca tccttcgatt ttgccttctt tcaggggcaa
     1261 aggcgcaata aaaaaagctt ttttggcagg ggttaagttg agcggagtta caatccattg
     1321 ggtggaaaaa gaagtagata aaggaaaaat tattgctcaa tatccggttt ttatcgaaaa
     1381 tgcaatgagt ctggatgaat ttgagcaggc aatccataac gtagaataca aactttatcc
     1441 gattgttata gaaaaacttc ttgaagataa agtttttgat tttcaggaat tgataactcc
     1501 aacatcaaac aaaaagtgcg gaggctgcgg gggatgtcag aattaaatca aaaattgaat
     1561 gttttggttt tgggatcagg tgcaagagaa cattcaatcg cactttcgat aagcaaatcg
     1621 ccgcttttga ataaattgtt ccttgctcaa accggtgcgt ttgaattggg agaaataatt
     1681 gaatttagag attttgagga ccttgctaaa aaatgtgtcc ttaaaaaaat aaatttggcg
     1741 gttgttggac ctgaagaacc tttgtgtgaa gggattgttg acgtttttaa aaaatataaa
     1801 attcccacaa tcggggttga taaatacttt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnggcaaaaa cgcccaaaca attttaatcg aagaattttt gcaaggcgaa
     1981 gagtgctccc ttatttctct ttgggacgga aaagaatttt tacattttcc tcccgcaaga
     2041 gattttaaaa aactcgataa ttccaaaaat gcaccaaaca caggcgggat gggggcgttt
     2101 tgccctgttt tgctcaacaa aaaacaacaa acaaagcttg acgaatacaa aaaacaactg
     2161 caaaacgccc ttaaaaaaga aaaagccggt tttgccggat ttatttattc ggggcttatt
     2221 tgggcacaaa aagataaaat ttgggactgg tatgtccttg aatacaacgt aagaatggga
     2281 gatcccgagt gtcaggcgat tttaacacat cttaattcgg attttcttga aattttattt
     2341 aaagcctcaa aaggaaagct taaaaattta aaaccgtctt acaaaaaaaa tacttccgcc
     2401 tgtcttgtaa ttgcctgcag cggttatccg caaaagccaa aagacggcga agaaatagtt
     2461 ctccccgaag acaaaaaaat tcaggtttat tttgcgggcg ttgaaaaaaa aggcgaaaaa
     2521 cttttttcaa aaggcggcag aattttatcc ctgtgcacaa atgcaaaaga gccttttagt
     2581 attttaaaag aatatgcaaa gacaatcaaa atgaagcaca aatattttag ggaagatatt
     2641 gctctctgac ataaaatgat gccgttgcaa gaggggtttc aaggttattt tttgcaaaaa
     2701 ccctcatata ataatacccg gcttcattta atacgatata gtcgttatag taataaacct
     2761 gatctttata tactttaaaa tcattggcgt aagcgatttt tgtgccccac cattcggatt
     2821 tctcgtcttt ttttagcacc tgaatcctta tgtaatctga ttttatgggt ttttcgctta
     2881 tgaataaata ataaattctc tcatcgcgct taaaattaac agacgaattc aaaaaattct
     2941 cttttgaaat cggcgaatga ttaaaaagaa ttatagtgtg gggttttctg cagcccgaaa
     3001 ggcaaataca aaaactaaag cacaacaaaa taagccaaac aagcctgaga ttgcttcgtt
     3061 ccttcaaatt aagcaggttt tgaaaattta tgtctcgcaa tgacgtaacc tttttatcat
     3121 tactatttta agctgtcgat tttggcttcg accgcctgtc tgattttagg atctttttcc
     3181 agcccgacaa atttttcgta actttgtatc gccttaacct tgttaccttc ggtttcatag
     3241 gcaagcccca gggcatagtg agcgtaggtg taattagggt ttaaataaac cactttttca
     3301 aagcattttt tgctttcggt gtaattagag ccttcggcat aaacaagccc taaattaaaa
     3361 atcgcatccg tgtaattagg gtcgatttct attgcttttt tatagtaaaa aactgctttg
     3421 tcgggattgt ttttgagttt gtaaagattc ccgagcttaa gacaagtgac agaatcatcg
     3481 ctttttaaat ccagtgcttt tgaatatttt acaatcgctt cttcaaatct tttttggtta
     3541 taaagagcgt cacccgattt tatcaaagtc tgaacatctt caacctgcgg ctcaacaatt
     3601 tttggcattg cggcaggagt ttgtacttca gtttcaatct gttgtccaaa aacttggggc
     3661 tgagtttgtt caattatttc ttctttaggt ttgtttttat cgactaaagc tttatggcta
     3721 agcctgtagt cttgattctc agggtcgatt tcaatagctt tttcgtaatt ttcgattgct
     3781 ttatcctcat agcccaaatt ttcgtaagct tttgcaagcg aaaaataagc ataagcctcg
     3841 ttaggattat aaattattgc ctcataaaag cttgaagcag ctttttgata atcgctgttt
     3901 ttcattaata tgtcaccctg cgaaagtagt gcgcttgaaa gattaatttt taatctgggg
     3961 ttatcttttt gcgccaaaag ctccttgtaa gtaggaattg cgctgtcata gcgttccatg
     4021 gcatgaagta caagtgcctt gtttgctttt atgtcgtaat cgcctttgtc tgtagctaag
     4081 acttcttcat aataattaag cgcattggaa tagtcgcctt ttgtatgata gcaaagtgcc
     4141 agttcttttt ttaccgctat atttgtgttg tctttggaaa gcgattttgt gaagtttaaa
     4201 attgctaaat caaggttgtt ttgtctttta taaacagttg ccaaattggc gtaagcattt
     4261 ggtgcttcgg gataccttgc cagaaaaatc ttatactgtt caatcgcagc gtcgtctttt
     4321 cccatatctg caagaaggtt tgcataatca aaccttaggg aagtaaaatc gggtttgagt
     4381 ttaaaagttt cttcaaattc tgtcagtgct aaatcgtctt tgtccatctg ttggtaagaa
     4441 acagctaaat ttgcgtgtga aacaacatct tcagggtttt gttctaccgc ttttttgaga
     4501 atatctattg cggtatcgta tttttttgcc ttaaaaagtg ccataccgta acttgcatac
     4561 tggtcaaaat cagccggatt ttttgcataa agcgcattgt attcatcaca tgctttttct
     4621 atctgattgt ctttaagata tgctcctgcc aaatttattc ttgaatcctg attgtcagga
     4681 taaatagcaa gaaatttgtt aaaattatct attgcttctt tattttcgcc catatagtac
     4741 tgaacgtttg ccatgttcag gtaattgtat ttgtaatcgg gaaaaatatc cattgcctgt
     4801 ttgtaagatt tataagcgtt ttcataatcg ccctgggttt gaagaatgtt tcctatgctt
     4861 atatagataa aagcgtcatt gggccgtagg tgaatggctt ttttgtaaga agcaagcgca
     4921 ttttgcaaat cgcccatatc agcgtaaagc ccggccattt ttgtatagag catcgcatcc
     4981 tcgccgctta tttcaatggc tttttgaatt aaagttatcg cgccttcata ctttttttga
     5041 tactccaaag tacatgcctg ctgataaaga gaatgagctt cctttgtcat cctcgcctgt
     5101 gcaggaaacg ttgtcagagc caaaattatc gccgatgcca aaagtttttt cttcatattt
     5161 taatcttctt cttttaaact ctcaagctga ttttaccaaa aaattagggc aggcgcaagg
     5221 aatattcaaa agaatatgaa ttattattaa taaggcggcg ggcaaaattt ttgcccgccg
     5281 ccaaaaaaag attaatcttc aaaataatca tcccacttat cgggcttttt gtaagcaaat
     5341 tttacataat tatcaagccg gttaacattt tgccgcgtca gcttaaaaag cgcaatcggt
     5401 tttttaaaca tggatttgca cagatatttg cccttttcaa gagaacagct cagtatttta
     5461 agcgggcttt cttcatttcc tattttagtc gtaaattcat tttgggcttt ttgttcaagg
     5521 ttttcgtcag ctaaaaattt tagtcccgag cgcgaagcat cttcgacaat agtctgatat
     5581 tttttgtgtt tatcgtcttc aatgttaaga ttttcttcca agttcaccct ttgataagtt
     5641 cttttttcgg gtttttccgt taccacctga attccgaaaa acaaaacaat aatatttatg
     5701 ctgttccaaa aaaggtttgc aataaccgca agaacactgt aaacatcaga gtatagaata
     5761 tcataaatcc cttcaagttg tgcggcgata agaattccga aaattacggc aagaggtact
     5821 gcatatttca tgtttgtttt agttgatttg gaaaattttt tgcttacaat aaatttaaat
     5881 tttgcaagtt tttcgggaat aaaaagtgcc tgaaggatgc ttccgattgt aaaaatagaa
     5941 gttataaggt cataaacatc ggcaagaata acatttttaa ttctcacgca ttgattaaaa
     6001 atgaaggcaa attttatcgt aaaataacaa atctgaaacg caaaaatctg ataaaaaagt
     6061 attaaaacag gggttatgtc aaaaaacaaa aataaaacag gcgcaagaag aaaaattatc
     6121 cttgcaatcg gagttataaa atacataacc cccgataaat aaaagaaagc ttgaatgggc
     6181 gtaagctttt tataatattc ccagtttttt ggcgtggtaa gaatctggac atttccttta
     6241 gcccagcggc atcgctgctt aatatattct tcaaaagtgg aagccgcaac acctattgca
     6301 atcggtctgt tgtaataggc tataccgtag cctgcggcat gaaacttaag ccccaaaaga
     6361 gaatcttcgg ttatgctgtt ttcggggaaa ttacccacag atgcaagatg agttcttctt
     6421 attaaaatat ttgtcccccc gcaaaaaacc gagccgtatt cgtttagcgc aggttcaatt
     6481 accctgtaaa acatttcctg ctcgtttgaa agaaattttt ccattttcaa gtttttttga
     6541 aaagcatccg gattgcaaaa atgctgaggc gtttgaacca ttgccatatt ttcatccgta
     6601 aaaaagtaaa cggtttctct taagaaagtc gaagccggca tgtgatccgc atcaaaaatt
     6661 accacaatat cagagtccgt ttgagaaagc ccgttattta tatttcccga cttaaatcct
     6721 ttatttgtgt ctcttgtaat gtaattgcac cctaaaagtt ctgttaattc cttaatctca
     6781 ggtcttctgc cgtcatctaa aacgtaaact tttttattgg gataatctat gtttttgcat
     6841 gcagaaatcg ttgttgcaag taaatcgggc gattcaataa tggtacagat aaaaatatcc
     6901 acgctcggac aaaagttttt gaaagttaca agttcgccgt ttttaaaaat atcctttgaa
     6961 tagtcctgct gaagtacatt gtccggctct gttttttcta aatttgaagc ttttacaaga
     7021 atcatactga aattggtaaa aacaattaaa gtcacaagct ctgctgccaa aagcgcataa
     7081 gaccaaaagg ttgtaaaagg cgaagttaaa agattaagag taaaaatcca acgccaagta
     7141 atataagcaa ccccgattac aaaaattatt gaggcaaaaa tcagaatttt taatgagggg
     7201 caagaatttt ttttgttttt attcatctaa atttccgtgg gttttatcac agttataaag
     7261 gaaacagcca aaccaaatta aattttgtcc gtaataatcg cttgtactgt cccaataacc
     7321 tttgtctttg aaaataggta ctaatttttc agtataaatt tctcttgcaa ttttttcatc
     7381 ggacatgttg ataacaggaa ttaaaattgc aacagaaccc gcgaattctt ctttattttt
     7441 taattttcct tctgcagtat aattaacata tattttgcct gattttttcc aattttctgt
     7501 gaaaaaatcc gcatttttca aaattttaaa tgctctttta tcgcctgttt tttcattatc
     7561 aaggaaaatc cttgcaaaaa ctcttatcgc atcgtaagaa aaatcgcttc tggttgaatt
     7621 ttcgagcaca acctgcccgt tttggattaa aaaccaatca gagggtaaac ctgttttcgt
     7681 tttttccatt gatttttcaa gataatagta agatgattta attaattcat cccaatcatg
     7741 ctttttgtcg tatttttgaa aaattctgaa agcataaggt gaaaaataag aaggattaac
     7801 ttcaaccttt tctaccaagg tttgattaac tcccggcatt aaaattcttt ttccgtcaac
     7861 aattttcgtt tcattttccc aaacggaatt tataatcgga agggcggttt ttagatattt
     7921 tctgtttttt gttttttcat aagccaaaat aagcgaaaat gctatatcaa tatcggcatc
     7981 cgatgccgaa tttttatcca aaattgtgta atcgcccgtt ttattttcgc cccaaagcca
     8041 gctgaataat ttatcatttc gttgaagatt gttttgtgac cagcttaaaa caaggtcaaa
     8101 agttttaaaa tcattcatta tcatacttct aagcagcata taagattgac cctcagaggt
     8161 tgtaatatta tctctttgag ggtccataat tcttccgtct ttggtcatgt aataaatttt
     8221 ataaaaatca taactgtctt taaggcaaaa agcgggctct tttgaaggtg ctttaagaat
     8281 aaaaagtgca acaagagaaa cgattacacc aaatattagt aaattaattt tttgcatttt
     8341 taaatccttt tatcactgca ctaatcataa actgatcgcg cagcaagttg gcataggtaa
     8401 agtgattata ttctgcgaca atatcaatat ggtcattaat cctataagtc gcataaggcg
     8461 aaactcccca tgtaagcctt gtaaaatcag gtgttattgc ttcctggata ccgccaaaac
     8521 cttttatgcc gtattttaga tgccattttt tgatttcgcc ttcgagtttt aggttaaggg
     8581 ttgtggcatt aaaatagctc gggctgaaat accctccgta gagttgtccg gttgaactgt
     8641 acagatctag cagattatat tgatagcttg aattgtaact tattatatca gtagaaaatc
     8701 tttgtaacca aggatttttg gggttattat accagagcct gcctaaaccg accatgcctt
     8761 catagtacat gtttgtaatc aggttttggg cataaatagc accgtaagag cctaaaagaa
     8821 atgcgtagca ttgtttaggc aattttattt gaaggtctaa ataaacttta ttatctgctg
     8881 cacgtcctgt aaaaataccg tcaataggtg ttcctactgc agataaatat gattgttcca
     8941 cattatttct tttaacccct aatttaactg caaaattatc gttaaaataa cgtttaatcc
     9001 agctgtctgt tataatcatt gcaccgtttc cgaattgaaa tgcttttacc cccaaatctg
     9061 ctcggtattc taattctttt gtcgggcgag cataaactcc gcctttaaat tcgtttacca
     9121 cattgttttg ctgatttaat ccgccggaac tgtaaactat tacgttgtac tcggcaaaaa
     9181 ccgtcttatt gccttctgcc gcctgagaaa catttactcc gaacttgtga taatctaact
     9241 taaactgctc tgccagctgt tgaaagaaga atgaataagt cggtgtaact gttattgctt
     9301 tgtcacgcct tatcttatac cttaaatctc tagcctcttt tgtgacactt ccttgcagtg
     9361 cggctttagc gttgtcaggc atgcccattt tgtagtaagt ctgggctcga agaagtttgg
     9421 tttcatcatt ttgaggcaag gaatttaatg caccaagagt gttgaaatca tccccgtttc
     9481 cgatgtatga aaaaactata ccttcttttg caggggtgta atcagggtct ttggcaagta
     9541 tattgttaaa aatatttctt gcggtttcaa actctttttt atctatttga atatatccca
     9601 gccctatttg tgccatttgg ttttcgggtt cttgtgccag aatatcttca taaatccgtt
     9661 gagaagcctc cgaatacccg agaagcctgt aagtttcggc aagtttgttt ttcatcagca
     9721 aattatcttt atcagcctga agcgcacggt aataatgtct gcttgccgat tcataatcgt
     9781 cgttgagcat tgccatatcg cctaaaatct gatacccctg cggtgtgttt tcaaggtggt
     9841 gggttttgat aattatatat gcatttcttg ttttattctg tgccataaga gaatttaaca
     9901 aagctttaac cgcatcagga ttttcgggat tttctttgta gagcggttca ataacctctt
     9961 gtgctttttc aaactcttta tttgccatat acatatttgc aagctcaaat ttgtattcgg
    10021 gtttggggta tttttcgatt aacttttcat aagataaagc ggcattttta aagtcttttt
    10081 ttatggaaaa aatagccgcg cggtttttta aaaatttttc cgaatcaggt tctgtcaaaa
    10141 gcagtttttc gttatagatt gtcgttttgt cccagaggtt ttgcgccata gaattttcca
    10201 gcgcaagaat taaaagcttt ttgtcgtcgg gatatttttt taacaaatct tctgttatat
    10261 caagtgtttt tttgtaattt tttgccctca gtgcttcata aagctggttt tctttgattt
    10321 cacgctctgt taaaactctt ttttggaacc ctttttgatg ttttttcggg tttaattctg
    10381 caagcgcata ggcatgactt tttttgacat gttcgcttct gtctccgctt gccataagtg
    10441 cttcataaac ttttgcggct cggctgaaat tgccctgttt ttcataaaaa ccggcaagag
    10501 agtattttat tccgttgtcg ttggggtttt gagaagaaaa tattttaaga aatttttctt
    10561 ctattttgta atcaccgaaa gagtcggata attttataat cctttttatt atggatgtgt
    10621 tttttgggtc aacttcataa tatctgagat aatatttgta agccgctttt tcgtctgaca
    10681 ccttctcgga tgaaacagta caaataacaa ctgtttcaag gtttaaattt ttaggggata
    10741 ctttatcaca ggcttttttt gccccaaccc aatcattatt gtaagagaac tgttttatca
    10801 actccagatt tattttttct gtctgtgaag gcttaaggtg tttatccttt tgaatttttg
    10861 aaaatttgtt tttatctaat ttgcctgatt ttgcttcgtt gaaatatttt ctgtaaaggt
    10921 aatattcgtc tttatacagt gcataaagtt ttgtgtataa ttcgactgct ttttcattgt
    10981 agcctcttcg ttcgcaaaat ccggcgtatt cttcaaggac ctgtttgtcg tctttgtagt
    11041 agtacattgt tttttcaaac agattaaacg ctgctttgtc attttttctt ttctccgccc
    11101 tttgtgcgct ctgtataaat atttgtttat aatctttttt gctgaaaagt gcctcacaag
    11161 gtgttgcgct tagtaaaaaa aatattactg atagcgtcaa aaaaatcctc attcggactc
    11221 caatgcatgt tcaaattata attgttaatt ccatactagt tataaaaaaa tgcaaaacaa
    11281 ttactcaatc gattaatacc catgtggcta ttaaaaaaca aaaaaatagc ttaaatttct
    11341 gcaacagaag gctttccggc aatacttaca agaagttcca aaacttttgg catttggcag
    11401 gcaaatgagt attgcccttt tgaaatcgaa cactttgagc agtcacaatt aagtctgtaa
    11461 cattcaagag cctgttctgt ccagttttgg gtaatggaat cggaaatttc gcctttgttt
    11521 tgcggaaaga attcttcttc cgcaaaatcg ctgaatttag caacattaac cgccatagac
    11581 aattctccct aattatccca tgctcagttt agaatatttt ttgcccgatg tactccacta
    11641 tttaacggat ttttttaagt gaataagtaa ataggtgaat aagtgaataa gttcaatttg
    11701 tcgctttgcg acgagtataa aactgttcaa cttatctact attcgacttt tcaactaatt
    11761 agtttcattt gcttcctgca aaagcgttga caaaacttct tcgcagtagc gtttgtctgt
    11821 tgcagaaaaa ggcaagacaa ctccgccaaa ttctttttga acttcattta aaacgttttg
    11881 gatttttgtt tttgggatat aatctatttt tgtaactatt gtcatcacgg gtaaattgtg
    11941 ggctaaaacc cattgtctca tttgaaaatc gtttttttga atgtcgtgtc tggcgtcgat
    12001 aaattggatt aaacatttaa tttgagggcg attgagaatg tattcttcca aatatttttg
    12061 ccatttatcc tgggcttctt ttgaaacttt ggcatagccg tagcccggaa ggtctgcaat
    12121 gataaatttg tctgaaaaat taaaaaagtt tatcagcctt gttttgcccg gagtgttgga
    12181 agtttttgcg actttattgt tgtttgcaag ggcgtttatg aaagaagatt ttcccacatt
    12241 tgagcgtccc aaaagaggaa attccgccag cttaaaatcg ggacactggc ttaatttctc
    12301 cgcacttact ataaactttg cacttaattg tttcattact tctcaatttt tatattgtaa
    12361 ttaatatctt tttaaaagct gtccgactaa aacgttttga tttttttgga gctggttgtt
    12421 tttttgaagc tcttttttat ataaaacagt tcttaaaaga ttatacattt tatcgctgtt
    12481 ataaacagta atttgcgttt ttttgtcgag aaattctgcg cttaccgatg cacgctttga
    12541 accgcccgag tttattccga caatctgaat tattttgtct ctgaaaaggc tcagaggttc
    12601 tcgtaaaaat atgtcaaatg ttttaaagat gttcatatat gtataatacc aaacaagatt
    12661 tttttttgct caaatattaa gaggaaatta ttaataatta caaaggagga tgcggaaaaa
    12721 ttaggaattt ttctcgcacc tccatagtcc agtaaacgcc aagccaccct tttaccaagg
    12781 ctgtgcttgg cgtttacctt cttaaggtcg ggcacggaaa acactaaaaa ttgtaaattt
    12841 ttgtattttt ccgtgccctc aaggggattt aaattgcatt cagagtgtct aaaaagcttt
    12901 tggtatcaat gtgttgtttc ccatgttctt caatatattt aagctgactt gctatatctt
    12961 tcttagccaa agttattttt tcttctgccg atgaagcttc caaaaattct tcaagggcat
    13021 tttccattaa ctcgccatga atttctgcaa caggagaagc gtgcagatat tttttataag
    13081 gcaatgctac agtcgggaaa agtgttaaat atttgccact gttgttaata tctaaaatcc
    13141 agtcatcaaa attgtgatta ttatcaattt ttttaataag ttcttttgat tttttagaaa
    13201 gactttttct atcagacata aaaccaatgg tttctttaaa gtgttctcct aaatgaatcc
    13261 caaaggaagg attagagtgc ttactgtaat tattttggtt ttgatagaag gcatttacgg
    13321 ttgatatttt catttttgaa acctcagtta gtgaaacatt tatataaaaa tcataaatca
    13381 attatttgct ggttttaaat atttttttaa tatttcttta ctattgcttt ttataatatt
    13441 acatccttta agtgagggtg ttttttaaaa aattcatctt gaagcttttg ttgttctttt
    13501 aaagcttttt cacgggcttt tttgaaatca aatttacttt gcaattcttc aaaagcggta
    13561 tggagtcttc tggtaaaaaa gtccaagtcg tctcttaaac aagttaataa ttcatgtact
    13621 ttttcagaag cagaggcaga gggttcggtt aagtgccaaa tacttatagg tttgtcaaca
    13681 aggtgttctg aacctttttt aagggctgtt atgtgaaagc cttgtttatc ttctgcggtg
    13741 cctataatcc aatcaggaag aattttattt tgtcttaaat gcattaaaac ttctttgaca
    13801 gcttttcttt gttcaagcgg aagctcaggt cttacttgtt tataaaagtt tttggcactt
    13861 gaatgaaagc caattccaaa agacggtgtt gaattaagat tctttttatt ttgttgtgaa
    13921 aagttgttta tgggggttat gttcacgata aaatctccta taatcgttta caagtgttta
    13981 aaggtacctg aaaaaaataa ttgccttaat tttgtttatt gttaagaaaa tttaataaaa
    14041 gggggttatt ttttcggcaa acacgttgat tttaactcgg acaaaatatt ttgtcaaaga
    14101 ggggcgaatt ttttttaaaa aaattcgccc ctcttgacca catggtctag aacacaagaa
    14161 ataattcaac catacggttg atgataacaa acgtatatat atagtaaatt atatctaggt
    14221 aaggtgtatt cagattaata gctggatgca ggaggtaagt ttaaaaagca ggggagtaag
    14281 gtatgtccgt agagattacg ggacttgaca taacgctaaa ccgcattaat acaatagagc
    14341 ggaattttca ggcactttcg ggagaattaa ataaacccga cagtgaattt caaagtattt
    14401 tagacggtta tatgcgaata aataacggtg aagatgtttc aaaagccgaa ataaacaatc
    14461 tgatagatgt ttattcagcc aaaaataatc ttgatgcgga ttttgtcaaa gcagttgtta
    14521 aacaagaatc aggttttaat gaaaaagcga cttcgcgctg cggtgcgatg ggattaatgc
    14581 agttaatgcc gggcactgca aaaagccttg gagttgaaaa tccatacaat gccgaagata
    14641 acataaaagg cggcacaaaa atgctttcaa accttttaaa gacttacggc gggaacaaag
    14701 aacttgcact tgcggcatat aacgcaggtg gcggcgcggt caaaaaatac ggcgggattc
    14761 cgccttatca ggaaacccaa aattatgtaa aaagcattat gagcatgtac caacgtacca
    14821 aaggaggtgc ttagatgttg ttaagaggac ttgcctctgc ttcaaacggt atgcaggcat
    14881 taatcgacag aaatgattct attgcaaata atattgcaaa cgttaatacc gtaggtttta
    14941 aaaaagaaaa tatggtattt aaaaacatat atgattctgc tgttgttgag aaatcggcag
    15001 acaccgatga agccaaaact ttgggtgaat tgagtatagg aagtcaagtc gaaaagctta
    15061 cttatgattt ttcgcaaggt gctttgagcc gcaccggagg gacttttgat ttagcaatag
    15121 aaggtgacgg atttttcaaa ataaaaaata ataacggaga agtttcttac acaagaaacg
    15181 gctcttttac tcttaataac ggcgggtatt tggttacaaa agacggagat tttgttcttg
    15241 atgatcagag ccaaaaaata agaataggtc ttgacggttt aaaaatgcat tcgctaaacg
    15301 acttgattat tggagaaaaa ggcaaaattc aggtaaataa cgaaaaaaat caggttaata
    15361 tgcaaacggt cggtgttttt gatttttcaa acaaagaaga catgacagca atcggcggtg
    15421 caaaatttaa acctacagat atagccacaa ataaagagct taaagcagaa aaatttacta
    15481 ttcagcaagg tgctttggaa atgtccaatt caaacgtggt tcaggaaatg attaattcca
    15541 taaacacttc aagaaactat gaagcacttt caaaaatggt taaaatgggc tcacagacta
    15601 tatcaaacgc tcttcagcta ggaagaataa gaggctaaaa taacatagga gaataaaaat
    15661 gttaaaagca ttaacaacag cagcaacggg gatgattgcc cagcaaaatt atctggacgt
    15721 gatttccaac aacatcgcaa acgtgaatac aacggcgttt aaaaaatcgc gcattgaatt
    15781 tcaggatttg ctcagtcaag cggtcaagac acccggtgct atgattaatc aaggaacatt
    15841 tcaacctgtc ggtatagaaa tggggctcgg ggtcaaaaca gctgctacaa ccagagtctt
    15901 tggtcaaggt gttgcaattt ccaccggaag aaatctcgac gttgcaatag aaggcgaagg
    15961 ctttttccaa atcatgaaag aagacgggac tcttgcctat agcagagatg gtgcatttaa
    16021 agttgatgct atggggcagc tttgtactac agacggcctt ttggttcagc ctcaaacttc
    16081 aatccctcaa gatgcttcag aaattaatat aactcaagac gggaatattt cggtaaaaat
    16141 aggctcaaac acaactcagc aaatcgtggg acagcttcag ctggttaaat ttcaaaaccc
    16201 gtcgggtctt ttatctgtgg ggcataacct ttatgttgaa actcccggct caggcagtcc
    16261 tattcaggat acccccgggc aaaacggtat gggacttatt caacagggat ttttggaagg
    16321 ttctaacgtt cagatagtag atgaacttat aagtcttatc aaagcagaaa gagcgtttga
    16381 atcgaattca aagataatta attcttcaag tgaaattttg aaacaaacaa acaatttggc
    16441 ataaaattat gaaaaaaatc attaacttag ctttaataac aattttgaca ttaagtgcgc
    16501 ttaagactca ggcgcgtact ttcagttccg atgaggttaa gaatgaaatt acccagcagg
    16561 ttattgaatc ttacaaaaaa tacacagacg cagatttaaa agcaactgtt cttgcggttc
    16621 cttttaagga tatggcactt cctgacggga aagtacgctt tgaggtgact tcaaatgtcg
    16681 ataaattcat ggcaagagat ttgaaaaagg ttaatattta tgtcaatgac aaatttgtca
    16741 gaacttttaa cgctcctgta gatgtaaaag cctataaaga tgttttggtt gccgcagatt
    16801 ttattgcccg agaaaaaagt cttacaagaa gcatggtcca aattaaaagg atggaggttt
    16861 cttataattt agaatatgca cttacagaaa atatgcttgg aaaagatata ataaccaaaa
    16921 aaacttttaa agaaggtgaa attatagata aaaggtttgt aaaattaaga cccgatgtcc
    16981 aaagaaattc agaaataaca gcttttttta aaagagacaa cattttaata tcggttgatg
    17041 ccatagctct ttcagacggg atgactggag attatatcgg agttgaaaac aaaagttaca
    17101 agaaaatata tacaggaaaa attataggtg aaaataaagt attaattgaa atgtgacata
    17161 attctcggcg aagaatgagc cttagaagta tgtcattgcc aggagcaaag cgacgcggca
    17221 atccaaaaga tgagaaaata aaaacaagaa aacgtgagaa ggataaatat atgaagttat
    17281 ttaagaaaaa tttaatagtg ttagcggttt tagcggctac ggtaagttat gtgagtgctg
    17341 aatccctgtt tacactcggg gcttctcaga cttattcgcc cgcgccaaaa tctttatacg
    17401 gcggggttca agctcggcaa gtcggagatt taatttcgat tataatgagc gaaagtataa
    17461 aaataagcga cagcttgact tatagcgcat ccagagaatc taccactaaa gataattttt
    17521 cgaatttaat aaattctttt atcggtaaag atgttataaa cagtaatata aacaatttcg
    17581 gcgggtcaaa cgaggtagat agttcggctt ccaattccag aaatatgtct ttcggggata
    17641 tgattgcagt gcaggtagtt cagcaaatgc cgaatggaaa tcttgttgtt caaggcaaaa
    17701 aaactattgt aaacggtaat gaaagaatgg acttgcttgt ttccggcgtt gtcgatccca
    17761 gatggataaa tgctgcaggt caggtttatt ctaaaaatgt tgcaaatctg cagttcgcat
    17821 cttccggtag aggttcaatc tcacgctcag gaaacgaagg cataataaac agagttataa
    17881 ggtacttatt ctaataaata aaatcgaaaa taaaatggtg gaggacataa aaatgataac
    17941 gacaattaaa aaggtgttaa taagcgcact tgcattatta ctgacggtga tgcccgcaag
    18001 cgcaataaat tccaccgtaa gaattatgga tataacccat gtaaaagggg tcagagacaa
    18061 tcaggtaata ggctatggcg tagttgtagg tcttcccgga acaggggaca actcacgctc
    18121 aactcagatt acaaacaaga tgctgttgtt aaatttaggc acggtaatcg aacaggaaaa
    18181 ctatattcaa aaaggttcga cagcagcggt tatcgtaacg gcaacaattc cgccgtttgc
    18241 aaaaaacggg gatagaattg atgtgacggt ttctactatg gcagatgcaa aaagcttgga
    18301 aggcggtgtt ttggttcaga caatcttaaa agcaccaaac ggtgaggcga tagcagtagc
    18361 acaaggccct ctttctgttg ccggagttaa tgccaaagca ggcgggagct ctaccagaac
    18421 aacaattacc acaaccggca gaatacccgg cggtgctatt atggaaagag atatggtaac
    18481 agatatcggt gatgccgaca ccgttaccct ttctttagat aatgcagatt atacacttat
    18541 ttcaagaata gcccaaacta tatccagtgt tattgctcct gcaaaagcat tggatggaac
    18601 aagcgttcag gtaaccgttc ctgaacaatt taaagatgac agagttgggt ttttatcaat
    18661 acttgaaaat cttgaagtga atcccacaat ggaaaaagct aaagttatag taaacgaaag
    18721 aacaggaaca gtcgttttgg gtgcagatgt aaaacttctt cccgcagcgg tcgctcacgg
    18781 gaatttgaca gtaacggtta caacaacaaa cgaagtttcc cagcctaatg cttttgcgca
    18841 aggttcaact gtagggtttt caaattctga aattattgta aacaaaactc cgggaagcgt
    18901 tattgcaatt ccggcaaata gtaatctgaa cgaccttgta agggctctta attcaatcgg
    18961 ggtaactcct gttgatttga tagctattct tcaggctttg aaaaaatcag gaagccttca
    19021 ggcagagctg ataataattt aagaggtaag aatggaaata aatcctttaa actatgcaaa
    19081 tatgacagaa acggtaagcg gcgagcagca aattcttaac cgtattaaag acacgccttc
    19141 ttcaaaagaa cagttgaaaa aggttgcctc ggaatttgaa ggtattttta tttcaaaaat
    19201 gttcagcctt atggatgaaa cagttgacag agaaaacgga attttcggag aagaaacaaa
    19261 acatttggat aaatttaagt cttatatgta caacgaactt ggaagacaac ttgcaagcaa
    19321 tcctaaaacc tctttcggat ttgcaaaaca aatttatgag caaatggaaa gacatttaaa
    19381 agactaggaa ggtaaattaa tggtaaattc aataggaata tcgggagtaa atcccattca
    19441 atatcttaat gcgacaaatg cttttaaatc tttgcaggca gaagtgcctg aacaaataat
    19501 tccggaattt tcagatggaa tcggggttaa ccaggaaaat cttattgccg aacagaacat
    19561 ggaagaaatt aagcaaattg tacaaagcat aggagaagaa aatatttccg atgatgatat
    19621 aaaatacgga gttacttacg gaagaagcgt tatagctgat tacgtagctt aacggagaaa
    19681 attatgattg ataatacttt gacaaaattt gaagaagcaa ttgatgaaga aataagagct
    19741 tatgaaaata tggaagacct ttataagctt aaacaggcta ttttgattca gggaaaaagc
    19801 gacgttcttt gggatgttga tgcaaaaata gtcgataaca tgaaaaatat aaggaatctg
    19861 aattccaaaa gaaaagaagt tgcaaaatat ttgggggatg aaaattttac aatatctgat
    19921 gttattgata aagctaaact ttcaaacgac actcttgcaa aaaaacttac cgaacaaaaa
    19981 gttaagttga atgtactttc aagttcactt tcgctttatg aaaagacaaa tttggatttg
    20041 ataaaacacg gacttgttat ggtcggcaaa aaactcaaca taattgtcaa tacggttttg
    20101 cctccgccta atcagtataa cagcaacggt aaaactatag gaaacgaaaa aattgaaata
    20161 agctcaatag tggaagaagc ttagggcttt aaaaaatagg agaaaaaaaa tgtctttaac
    20221 ttcaagtatg aatatagccc aacaggcact tttggttaac gaagccgcac ttaatgttgt
    20281 gtcaaataac atttcaaatg ttaatactat aggctattca aggcagagag tttgtttatc
    20341 ggcaaacgtt aatcatacct cttttggagg ctcggcttat ctgcaggcat attcaggaac
    20401 aggtgtaaat attgacaggg tagaaagata tacggatgct tacctgcaat catattacag
    20461 gcaacaaaat tctctttata attatttgaa tcaggcagct aatatagcaa gcaacgttga
    20521 agatattatg aatgaattaa acggaacagg tctgcaggca gcatttacca gcttttttga
    20581 agcagctcaa actcttaatt taaatccttc tgatatttcg gcaagacaaa attatgccca
    20641 gcaggtgcaa accgttgcaa tgaaatttaa tgaattgggt tcaagtctta aagatgcaag
    20701 aaccgctctt gtcggggatg ttaatgaatt cggttccctt cagtcctcaa aaatttattc
    20761 tttgactaca ttggcaaatg agaaattgca agagatagca aaagccaatg ccgatattgt
    20821 aaaaataagc tctccaggta tgcttccaag cggcttgctt gatacccgag atcagctaat
    20881 tgaagaactc tctcaattag ttcctgtaag tattgtggaa aacagtaacg gctcggtcaa
    20941 cctgtcaatt aacggaattg agatgataaa aggttctcag cagacaggaa cgctggatgt
    21001 ggtggccggc agtcaatcag aacctgcaat aatcagggtt aaagaccttc aaggcgaggt
    21061 tgtctatagc aatataaatt cttatcttaa ctccggctct atcggcgcgg ttattgatat
    21121 ggcaagcgaa gaaggagatg tattaacaat aaaatctact atgaaaaatt tggacaaact
    21181 tgccaaaggt ttctcagata ttattaacag aatacaaacg caggttatag atggcaaaac
    21241 atctcttgca atagataaag acacaatgct gttaacagat gctaccgaaa atatatttga
    21301 aagcagtgac ggagaagaaa ttaacgctct taacataagg gtaaattcag atattttaga
    21361 agatccttat cttattgctg ctgcaagact tgataaaaca gatcctaatt acagtgcagg
    21421 tgcaataggt aacaactcta acatgttaat ggttttagat gtaagaacta acggttcatc
    21481 cgaacttgat aatttttcac cagaaagctt tttgtcacaa atggtcggcg atataggctt
    21541 aaagactgca agcattaatt caagcttaaa aagtcaatcg gcagtattat cacaagtttc
    21601 cagccaatta tcttcggcaa cgggtgtaaa tattaacgaa gaagttattg atttaactaa
    21661 atatcaagcc gcatatcagg catctgcaag agtttacagt gtatgtaacg atcttcttga
    21721 tgtattgtta aatttgggca gatagaaagg aataaaaaat gattttaaga accacaaatc
    21781 aggcaacaaa taacgctaat ttaaattata ttttttctaa tcaaattaag tataatgaga
    21841 tttctcagca aattgcttca cagaaaaaga ttcttgtacc ttcagataat gccatagatt
    21901 caatgaatat tttgaatata aacaggcaaa tgagcaaact taacggatat ttgaataata
    21961 tgtctatggc gcaaaacgag ctaagcgttt tggacaattc tcttgcttca ataacttcaa
    22021 gcctgcaaaa agcaaatgac cttgcggtac aggcagcaag cgaaacaaac ggaacaaaca
    22081 atcttcaaag catgaaggtt gaaattgatc agataataga gaatgtaata agtttaggca
    22141 atacaaaata taacggacaa tacatttttg ccggtacaaa taccggaact accccttttc
    22201 aagcagtttc aacaggcgga gttgaatata cgggaactcc tcaagacgga ccttatcaaa
    22261 ggaatattga aatatctgat ggagttcttg cgcctataaa cgttccgggc gacagacttc
    22321 tgggtagtta tgatgcggct acagaaacag gcacaggggt tttaaaaact ctttatcagt
    22381 taagcgatgc aatgggtgct gttccacctg attttgacac aatcagggca agtttggacg
    22441 aaattcaggg cggaattaca tccacaacaa atataaggac aaactttgcc gcaatgaccg
    22501 caaggtttaa aatgaccgaa acgtccgtta ataatagtct tttgcagctg acaagctata
    22561 aatcacaaat gcaggatttg gatttggcag cggcagccac aaatcttgca aacactgaac
    22621 ttgcacttaa tgcttctatg gctgttgctt caaatgttat tcaacaatta agtcttttag
    22681 attatatgta aatcttttat aaacaagcaa aaacttacca tcagggcgca atgcgccctt
    22741 ttttttataa aaatggtgtc tgggattaat aagccctatc atttcctccc ctcttgataa
    22801 aaaatggtgt ttagtgtaac tttattttag aaatgggctc ttgaaaaatg gacaaaaaaa
    22861 ttgaattgga cttggatgct gttgttgccg agtgcaggtt aaccgaagat actgaagagg
    22921 atattttaat caagaatatt gacaatcttg agtcaattta cgatggaaaa aatcttgttg
    22981 caatttacaa taaatttttg accgttgcca aaagccccaa tattttgatg tatttgataa
    23041 gatgtgttga caaatacagg gataaatctt ctctaagttc gcttttggac ttgctttttg
    23101 acaaaaaaaa ttctgatggt gttcgtgtta tgtgtgcaaa agtcattgca aatttaaaag
    23161 acacaagtgc cgtaacctcg cttttatact gcctgaacga caaagaagaa aattataaag
    23221 taaggctggc atgtgccgat gccttgggca gaataggcga caaatatgcg gttgcgcctt
    23281 tgattaacgt tgttcaggat gaggatgaaa aatctgttta tgtaagagaa tccgctgccg
    23341 tggcacttgg aatgttggga gatgcaaggg cggttgatcc tttggtctcg attttggaga
    23401 caaaaaaggg atttgtggat aaattcactt ttttaaaaga aagaattata gaggctttgg
    23461 gtaaacttaa tttgagcagc gatcgagttt ttaatgctct tgaacatgct ctttgcgatg
    23521 aatctcatct gataagaatt aacgcgatag aagcacttat ggaatctgag gatgaaagag
    23581 cttacgggct tataaaaaaa atgttggagg atttacacga agaagtcaga aaaaatgctc
    23641 ttatagctct ttacaatatg gtcgggcgtg atattcttga tgagataata agcggcgaat
    23701 acgatcaaag tttgaaaata gaagcccaag ccatgattga agaatatgaa acaggcgagg
    23761 aagagccatg ataaaatcaa taattttact ttcaggcggg ctggattctc ttgtaagcct
    23821 tgctgtttca aaggaaaaat ttaatattga acttgctctc acttttgact atggtcaaaa
    23881 atcggcaaaa aaagagattg aagcctctga aaaaatctgc aagtactaca acataaatca
    23941 aaagattatt aatcttgatt ggctcaaaga aattacgcaa acttcgcttg taggtgataa
    24001 gcaaattcca aaagtaagcc gacaggattt agcctcggat gattttgttc aaaacagtgc
    24061 aaaatctgtg tgggtgccta atagaaacgg actttttttg aacattgccg gatgttttgc
    24121 ggattctttc ggatacgatt atataatttt tggtgcaaat aaagaagaag ggcagacctt
    24181 tcctgacaat acggaagaat ttgcagaaat tataagtcag gaacttcaat actctaccct
    24241 taatcacccc aaaatccttg cccccttgat taattcaaac aaggatgata tagtaaaact
    24301 tgcgataaag cacgctgtac cgcttaattt aacaagaagc tgttattcag ataaacaaaa
    24361 gcattgcggt caatgtgaat cgtgtataag attgaaaagg gcacttgaag ctaataattg
    24421 ccaaaattta atcaaagaat tattttaaat atgcatacaa aatttataga aaaagaaatt
    24481 aaatatacag gggctgaact ttgtcctcat tggatttaca gaacttttaa gctgcaagga
    24541 gatgccgttg ttgccttctg cggcgaatgt gaagtgaaat taaccgaaat ggtggacatt
    24601 gaagatgtaa taaataatga accgatttat tcaaaatcga tgctgaattt cattgtcgag
    24661 cattttaata caggacttat tgaaggagtt acaagacaaa ggcttttaat ttgtataatc
    24721 aaagaagaat tggaaaatct atttactatt taccatttac catttactct tcaaagaaga
    24781 ggcgatgatt tatttatcga taacaaaaaa ctttcggtat cgattgcgac aaaatcttcg
    24841 acttccgttt tgatgcatat aggagttaat attgaccccg aaggtgctcc gattgaggct
    24901 atcgggctta aagatttggg aatttccgat ataaatgatt ttgcacaaaa aattatggta
    24961 aaatattcta aggaaattga tgatataatt ttggcaagta ctaaagtggt cgggcgctga
    25021 gttaaggaga acggatgaaa acaccggatt atctgagtta taccagttat aaaaagaaaa
    25081 aacaaaacag ttcaaaaaaa agtatggtaa tttttgtaac cacttttttt gcgacattgc
    25141 tggtttttac cgctattgcc aaacagctta cgcctaacgt tgatgttaca atcggcgaag
    25201 atacagacat ggaagcaaaa gaaacaggtt tgggcgtaaa aagatttata gatgacagat
    25261 taaaaatgat tcaaatggaa gacaattccg caggtgtttc caagagagat tctgataaga
    25321 gtattgcctc agcagaagat gaaaattttg acagttttgc agaagattta gatgaaagag
    25381 tttatttgcc caagaaaaaa cctgcaaaag aagaggttgc agaaaaaccc gcaaaagaaa
    25441 ccgtaacacc gccaagacca aaaccaaatg atgaaataaa cccttttgat gcaccaaaaa
    25501 tgtcaaaagt tttcgtgggg tattattctt ctgttgagca ggcaaaagta gctcagggga
    25561 ttttaatgga tgctgatttg ggagttactc catttataaa aaacatgggc ggagctttta
    25621 cgcttcaagt gggctcatat tcaagcaaaa taaaggcact tgagcttgca aacgaacttt
    25681 tgagaaataa tttccctgca agggttgttc aggaataata tagtaaatag taaatagtaa
    25741 ataggaaata gagtttgcag gtcaggcagt gcctgatttt ggtatgtttg taaagtgttg
    25801 ctaaactatg gataagaaat ttttatttcc tgcttttgtc ttaagatgtt ccagccttga
    25861 ttttacaaac gaagcgtgtt gtgaatgggg tttttgaatt aaaaactttt tgtaatacct
    25921 ttgtgcatcg gcatttttgc ccattttgtc aaagcagatt cctattccaa gatatgcttt
    25981 gttataattc gggttgagtt tgagcgtttt gtggaaaatt aagcttgcct gagtataatc
    26041 tcccatactc atcaaaagtg ccgatttgtg attgtaggct tttaaatatc ccgggttggt
    26101 ttcaataatt ttttgataaa tcataagtgc catatcgttt tcttcgacta tttcatgggc
    26161 gattgcaagc tgaatttgag catttaaatt gttagggttg agtttaagcg attttacaaa
    26221 gttttttatg gcacagcaac tctggtttga aagcagataa caaatcccaa gttcataata
    26281 aatatttgaa tcgaattctt ttaatttttt tgctttttca agtgcgcaaa ttgctttatc
    26341 aaggttatta agatgcttat aacaaactcc aagtccatag taagattcgg gattgtttct
    26401 gtcaataaga attgcattaa gatattttga aacagcctct tgatataagc ttgcaagcct
    26461 cagagcatct gcttttacgc aaagttcgta agccaaagca ggcttgtcta aaatcggatt
    26521 tgtgcaaaaa tctgaaatgt tttttttagt ctgacaaact tttattgcca aaatcttacc
    26581 tcaatccctt ataaatataa gtcatataac gacattctat aagctttttc gggtaaaaat
    26641 aatgaccata aggtttaagt tgttatagac cattggattt atatttttat tattattttt
    26701 tttgactata ataatgttgg gggaattaat gaaaagattt ttaatcttat ttgtaatatt
    26761 tatttttcaa atgattttta ttctgccctg ccatgcaatc aaaataggct tgcagacagg
    26821 tatggacagg gtttatatcg gctcttccac tcaagcacaa attataaatg gcagaacaaa
    26881 caggcttttg tacacaatta acggcatgaa agcttatgaa gtaagggctt acagaagcgg
    26941 aattgcgata aaagttttgg gaaaatattt taatctggat tctgattatg ttgtcgtgaa
    27001 accttctcag ggcgggttta taagtgttaa aaataaatgg tacaggggtg attttataat
    27061 ctgcaataga aacgggaaat tgaatgttgt caatgatgtt gggatagaag attacataaa
    27121 aggggttgtg ccttctgaaa tgccttcaaa atgggcttat gaggcacaca aagctcaagc
    27181 aattgccgca agaagctatg cgcttgcaaa tttaggcaaa agggcagcag gcggctatga
    27241 cttgaaagat actcccgaag atcaggcata cgggggggct tcggcagaat cggagcaaac
    27301 taacaaagca gtttcagaaa caaagggaat agttttgatt tacgatttaa aaataatacc
    27361 tgcttattat tcggcctctg ccggcggaca aacttcaaca tcatcggaag tttggacaaa
    27421 aaacttgccg tatttgcaat cagttccttc ttttgacgat aatataagga aaaacgggca
    27481 tggggtcgga atgagccagc atggcgcaaa taatttagca aaaagcggtt acaacgctta
    27541 tcagattttg cagtatttct ataaaggcgt gcaatttgcc agaattaacc ctgaatatta
    27601 taagtagttg aatagttgat aagttgaaaa gttaaattta ttataattgt acttatttac
    27661 atctatttac tatttaccat ttactatttc cttcaaatta ttaactttta gtaatatttc
    27721 gggttatttt tttgaaaaac ccttgccaaa actggattta tatatataat aatcttattg
    27781 ttccctgtat ttgggggcat ggtagtacaa acaccggaga ttgacatcaa tttaagtcgt
    27841 ttttgatgaa tagagagtaa aggttttctt ttcatgaaaa tgtgtcaagg atggtaaaaa
    27901 aggaggttcg ttatgaacaa agaagaattg gtaaaagaag tatcaaaaaa agcaaaagtt
    27961 tctcaaaaag ctgctgctga cgttattgcc gctgcattgg acacaatcca aaaaacagtt
    28021 tctaaaggca aaaaagttac tttggtaggt tttggaactt gggaaccacg caaaagagct
    28081 gctagaatgg gtcgtaaccc acaaactggt gctcaattaa aaattgcagc taaagtcgtt
    28141 cctgcttttt cagccggtaa aaaattcaaa gaattggtta agtaacctga ttcttttggt
    28201 tttaaagcgg cgagaaaaat ttttctcgcc gcttttttaa aaaaaaaatt attaattaaa
    28261 tagcaaaaat tttttttaga ggatttataa atattaatta gctgtaggag tgcgaaatga
    28321 aagttttatt taacaataaa tataagactt ataatctaaa tttaaaaaat tcagatacaa
    28381 gaaacaggtc atttttaatc caaaaacccg atacggtttc ttttggtatg gcaaatatcg
    28441 gtgatgacgg ggattttgaa ttaaatcaaa taagagttaa tcaaaaaagg tttgaaacaa
    28501 atataagaca gcatatagtt tctatccctg acactctttt gtttttacag gatcataatg
    28561 gtgttacgca atttatgagg gcactttatg atgaacatcc gaataatgct atgagaattt
    28621 tagattatgt taaggatgca aattttgatt taaaagagaa aaaaaagttt tttgaattaa
    28681 aggataaaga tggaaatagc caatttgatg tcgctataaa aacaaaatat tatgaaattg
    28741 caatgaaact tcttaaactt atggcaaaaa ttaatcctga aaaagttgtt gatttaaata
    28801 ttcctgaaga aatttataag atgataagtt tcccaagagt agtggatatt atgattgaga
    28861 atgaagctct gtcaaaagaa caaacattaa agtttttgga taaaaatatg gaatatgttc
    28921 ctttatatga aaaaaggcgt gctcttgttg aagattatgc agatatttat ataacagatg
    28981 aagatataag tggtgctaac tgggtttaat ggagcgggag acgaggctgt cttgctgcgc
    29041 tcgcattaaa cggcaacgct cacaacaaaa tcgaaaagca tcgtttccga ttattgttgt
    29101 tccctctctt cctctcagaa aaagatactt aatctttttc ttcggcagag tgctcgtgcc
    29161 tctgctcgct tgcgcttctt ggattattcg gggtgtgcca aaagcgtggt tttgtcacac
    29221 cttacgggct gggttcgctc acgctcacac ggcgccctac cgaaaatccg ctcgaactca
    29281 aaatgagttc tcgcctctat ctctacaaaa attaaaagcc cattccaaag atggaatggg
    29341 cttttaatgg agcgggagac gaggctcgaa ctcgcgacat cttccttggc aaggaagcat
    29401 tctaccactg aattactccc gcgtatcttt tattttatat tatcaaaaaa aatttgcaag
    29461 aaaaaacatt atcaagatta attaattttt gtttcacttt gctttaaaaa aaaattctta
    29521 tgtattaaaa ttaaaaaacg aataaaaaag gatttaatca tggctgtgaa tactgcaaaa
    29581 aaaattgatg ataatttaat acctcaaaat attgaggcag aagaggcggt tttgggggca
    29641 attttggtta accccaatac aataaccaaa gttgttgaat ttttaaagcc cgaaagcttt
    29701 tataagcccg cccacagata cgtttatgag gctatgcttc agctttttaa ccaaaacgag
    29761 cgtattgatc ttgtttctgt atccgatgtt ttgaacttta acgcaaagct tgaagtaata
    29821 ggcggaagag ccttcatcaa tgatttaagc tataagacaa taacaacttc aaatatagaa
    29881 tattatgcaa aaattgttca ggaaaaagca attaaacgct ctttgattaa tgcaggttct
    29941 gaaataatca ctttcggtta tgaccttaac cctattgatg aatctctgga caatgccgaa
    30001 aaacttattt ttgacattgc ggcaaaaaaa acaacaaaag accttcttca tgttaaggat
    30061 ttggtcttgc aaagctatga aaaaatagaa taccgctaca atcacagaga agaaatgaca
    30121 ggtgttccaa ccggttttta tgaattggac aatattacaa gcggtcttca aaaatctgat
    30181 ttaataattt tagctgcgcg tccttcaatg ggaaaaacag cattggcatt aaatattgct
    30241 caaaatgttg ccataagggc aaaatttccc gttgccgttt tctcgcttga aatgtcaaaa
    30301 gagcaacttg ttcaaaggat gctttgctcg gaagccgaag ttgacagcca aaggctaagg
    30361 acagggcata tgcaaagcaa agattgggaa aaacttgcaa atgccatgaa cgattttgcg
    30421 caagcaccaa tttatattga cgattctccg ggctgtagcc ttaccgatat aagggcaaaa
    30481 tgccgcaggc ttgccatgca ggaaaaaaat ctcgggttga ttgtaatcga ctatttacag
    30541 ttaatggaag gttccggcaa agaagagaga attcagcaga tttcggctat ttcaagaggt
    30601 ttaaaaacac ttgcaagaga attggacgtt cccgtaatcg cactttctca gctttcacgt
    30661 gcggttgagc agagaaaaga ccgcagaccg atgctttctg acttgagaga atcgggtgcc
    30721 attgagcagg atgccgatat tgttatgttt atatatagag aagattatta taacagagaa
    30781 gaaggtgacg aaatgccaaa agccacaggg aaagaaggca aatctgatat tttggtcgca
    30841 aaacacagaa acggacctgt cggaaatttt gaacttatct tccaatcaaa tattacaaaa
    30901 ttcaaaaatc cgattaaaac ggatgtattt taattagttg ataagttaaa agttttttaa
    30961 aatttctcat aagagccgac caagcggaag taaggggtat gtttttggat ttgtgccaga
    31021 gcttctgcta ttttgtcgtc taaaatatgc ccgtcaaagt caacaaagaa agtgtattca
    31081 ccgaaatttg tttttgaggg tcttgaatct atgtaagaca ggttaatttc aaaatcctga
    31141 aatattttca aaatatctaa aagtgcaccc ggcttgtttt ctgtcgaaaa agcaatagaa
    31201 gtctcatctt tacctgtctt tggggtattg taatttccga taagcgcaaa tcttgttttg
    31261 ttgtcttgct cgtcattaat attgtccaat aaaacattca ggtaatatat atcagccgtt
    31321 ttttgggtac ctattgcagc gtaagtcaag ttgtaatttt gaaggctctt tgccgcttct
    31381 gcagtgtttg ctacttctat tatatttgtg tgcagcggaa gtttatcatg aataaaattt
    31441 tgacattgtg aaattgcctg cggatgagca atcacgccgg ttatactgta aagctctgtt
    31501 gtttttgaaa gcaggcagtg ttttattggc attataagct ctgcgacaac ttttatattt
    31561 gggttttggg tttttataag actgtctatt gtttctctta ttgtcccttc aatggaattt
    31621 tctatcggta aaaccgcaag cgaatatggg ttttcgtcaa catattcaac cgctttttta
    31681 attgatttca tcgggacaag ataagggtca agattatatt tttggcagaa aatatctttt
    31741 gccatttctg taaaagaacc gccgggtcct aaatatacaa gactttcaac tctttctaaa
    31801 aacgtcattt gctaaaaccg ccttctttta gtataattac tattattgta caggaaggtc
    31861 aatagaatgg caaatattaa gtttgggaca gacggctggc gtgcggttgt tggggagggt
    31921 ttcaattctc aaaatgttga aattgttaca aatgcaatcg ggaaatatgt ttttgataat
    31981 ttcggaatcg acaaaaaaat tctcataggg catgactcca gaaatatggc ttttgaattt
    32041 gccaatcaat gcgcaaattt gcttgcttca aagggttttg aaattctgat gagcaaaaaa
    32101 gttttgccca cgcccattct tgcctataac gcaaaacata aaaacgcctg tgcgataatg
    32161 tttacagcct ctcacaatcc gccccaatat ctgggaatga agtttattcc cgattatgcc
    32221 ggcccagcta cagatgaaat tacagacgag attgttagaa atattaaatc aaatcaaact
    32281 attcaactat tcgactgttc gactgttcaa ctcaccgatt ttatgcccga ttactttgaa
    32341 cacttgaaag aattaataga ttttgaaagc ataaaaactt taaaaacaaa cataattttt
    32401 gacgggcttt attctgccac aataggttat tttgacaata ttttagatga atttgaaata
    32461 aaatacgatt caatccacat gtttcacgac aaaaatttcg gtggagcaat gcccgagcct
    32521 aagcctaaat atttaaagga attaattgaa aaagtaaaat caaatccaaa ttcaatcggg
    32581 cttgcaaacg acggtgacgg ggacaggttc ggggttataa atgaaaacgg tgaatgggtt
    32641 tcgccaaatg aaattattgc aattttgctg ttgtatttaa aaaattataa acacttaaaa
    32701 ggtcctgttg taaaaacagt cggcgcaagc cttctttttg acagaatagc atctaaaata
    32761 ggggttgaag taattgaaac ggccgtaggg tttaaatttg ttggtgctgc catgagagaa
    32821 tttaatccca ttatagcagg cgaagaatca ggtgggctga gcattcaagg gcatatcccc
    32881 gaaaaagacg gaattcttgc aaatcttttg atattagaag ccatggctgt tatcggaaag
    32941 tctttggttg aactccagcg cgaactgtgg tttttggtcg ggacaaaatt tatcaatgac
    33001 agagttgacc tgcatttgca aaatgatgag caaattaaag ccgcacttga aaaagtttct
    33061 gcgataaaaa atataggcaa atttaaagta atcaaaacct gcacaaaaga cggaataaaa
    33121 ctttattttg acgacaattc aagctgggtt ttggtcagag caagcggaac agaacctttg
    33181 ctgagaattt actttgaaag cgataatcac gataaattgg agtttttgaa agaaagtttg
    33241 aaaaaaacac tttcggcttg aaaatccttt tttttggttt atcataaaaa aagtcgatta
    33301 ggtggtaata tggtttcggt caagctacaa acttttgcaa aatatttgga tcagcctgct
    33361 ttggtaaaac acttggataa aaaaatgcct gttgttttat ccgccctggg acttgcttac
    33421 gggataaacg acagttttca agcacccaaa gacgagcaat ataaaacctt tattcaaaat
    33481 ttatctgtat tatcgcttac aatcgtttct gctcttgcag ctacaagagg acttaaactt
    33541 ttcggcaaaa aaattgaagg acttattgaa cttccgcacc ttcacaagaa agacataagc
    33601 gaagttttga aaaaaattac cgatgataaa acaaaaaaac ttattcaaaa agttttggat
    33661 gataaaattc tcaaatttca ggaagttaaa aatttaaaag aaaatttaaa aaaattatac
    33721 gatataaata taaataaaat catccctgat gcccacaatc acggtgcatt tgaggagttg
    33781 ggcaaacttt ctttattggg cttaattcct gttttgggcg gaattacagg aggggttata
    33841 ggtgacgttt tgaccaaaga caattggaaa aagaaattcc caaataaaat taaagaagga
    33901 acttatcagt atttgaacaa tatttttttg tgtaatgtcg gggcaggaat tgcacttgct
    33961 acaatgaaca ggcttaaaat acaatccaaa atcgcaagat ttggggctat gcttgcagga
    34021 gtcgtgggag tgggtcttat cgcgggaagt gctattgcaa attttatagg caaaaatttt
    34081 atcaacccga tttttgacaa aaaagaaatc ccaaaaagag attttcactc aatgacggca
    34141 aacttgaaca gcgaaaggca ccctgaagca cttgatttga gtctgcatat tgacgatatc
    34201 gcatcggtgg gatttttatc gggtttaaaa tggataggtc ctgttcttcc gcttttgtat
    34261 tcggtttctg catatagggc aggaatagga tacagaaaca gcaaataatg gtttttcaaa
    34321 atttaaaaaa taagtttata attaaacttc tcggcaatgt caagaagctt ttttagttga
    34381 ataatttata aaaaggagaa attttatgag tgaatatata aataacataa gatttgacgc
    34441 caaaaaggcg gaaaatttct ttgcccaaag actagcttat actcttggtc ctgtagaatt
    34501 gagtgaaatg atgaaagaag gaaaagtgaa attgatagac gttagatatc ctgatgattt
    34561 tgtcgaagga cacattcctt ttgctgtttc aatgccgaaa atagagttgg cgcaaagatt
    34621 gtctgaactt tcaaaaaaag agcttcacgt ggtatattgc tacaatcagc agtgccacct
    34681 ggcatcaagt gccgctttaa ttttggcaaa aaacgggtat cctgttatgg aacttgaagg
    34741 cgggtttgat gtttggaaaa acggatttaa gtttgatgtg gaaaaataag attttttatg
    34801 ccacttttgg tggcaaaagt ggcgcaaaag ccacgacctg catctgcgct cattaactat
    34861 cactaaactc aactcaagct cgcgaactcg ctacgatttt tgtattactc ttgatgtttt
    34921 acaaaaactc gctcaaacac actcgctttg ttgttgtttc gttaagtgat agttattcgc
    34981 ttcgataagg tcgaaaaaac ggggttcggg gcagagccct gacgctttga cattttgttc
    35041 gcgagtttct ctttcttttc gtccttttct ttctctttgc atcgcaacaa agagaaagaa
    35101 aaggacaata taaaaaggca catccgcaaa agctaaaaaa caataaaaaa ataatataaa
    35161 ataagattta aaaccaatca ataagtgcta attgttcgcc gaaattaatg tttaaatttt
    35221 gggtaaactc gttattaagc aaataatcca accctacatt ataagaaagc ggctcttgct
    35281 ttatgccgga ttttctccac aatttttttg aaattgtgcc gcgttttttg tcgtacacaa
    35341 tactgtaatt taaatcctct ctggcgcagt atggaatttg aaaagtttct tttttgtctt
    35401 tgtttgtaaa cattgtcata agtccgtggt ttctgcagat aagccccctg taatcataaa
    35461 ctgaacacct tttgtcaatc aaaaaaggac attcgtacat gaacttttct ccgttttcaa
    35521 gtgttttttg tttttctttt tttaattcag agattttttc gcggattgtt tttttggaag
    35581 cttcgtccag tttcataaac cccgacatca gatattgcat ttcaagcctt gtaaaaggat
    35641 attctccgca tttgcagcac agcgaacagc cttctttgca gtggatgtaa ggcttttgct
    35701 gcttaaaata tttatccaaa taagagccca ctatttctaa atatttttca tatttttcaa
    35761 tcatatctaa tattaaacaa caaaagcagc aaaatttgta attatgcctt gagaatgtta
    35821 taactaagat aagagcggaa gaagagttat ctttcgcaat cttagaaaga aggaattatg
    35881 gaaaaaaaca acgaaacact acatgtgctc aggcactcta cttcacacat catggcacaa
    35941 gccgttcaag ccttgtttcc cggtgccaga cttgccatag gaccttcgac ggacacgggg
    36001 ttttactacg attttgacct gactgacggg catgccttca cagaagatga cctgataaaa
    36061 atcgaagaaa aaatgaaaga aataatcaaa caaaatctca cattcgaaaa atattttgtt
    36121 gaagatgtcg atgctaaaat cgccgaattt aaaaagcagg gcgaaattta taaagcagag
    36181 cttctggaag aacacagaaa cgacaacccc accctgtatt taaccaaaaa caaagacgga
    36241 aaagttttgt tcaatgatct ttgcgcagga ccacacattc cctattcgtc ttttatcaaa
    36301 cccaacgctt ttaaactttt aaaagttgca ggtgcttact ggcgaggcag tgaaaaaaac
    36361 aaaatgcttc agcggattta tgcaacggta ttctggacaa aagaagattt agcagattac
    36421 ttaaattttt tagaagaagc agaaaagcgc gatcacagaa aattaggaac cgcactcgac
    36481 ttgttttcga caagagaaga agttggcggc ggacttgtac tttggcatcc gaatttagca
    36541 gtcgtcagag aagaagtcga gaattattgg agaaatgagc acagaaaacg cggctatgtt
    36601 atcgtaaaca ccccgcacat cgcaaaatca aaattatggg atatttcagg ccacacagga
    36661 cattaccgcg ataacatgtt ttttattcac gaagacgatg aagaatacgt cttaaaacca
    36721 atgaattgtc cttttcatat catgatttac cagtcaaaca gatactctta ccgctcgctt
    36781 cctctgcgta tggcagagct cggtactgtt tacaggaaag aaaaatcggg agcattatct
    36841 ggattaaccc gcgtacaggg atttactcaa gatgatgcgc acgtgttttg cactcccgaa
    36901 cagttggtgg aagaaataaa taaaattatt gattttgttg cagatacaat ggcaattttt
    36961 aatatggaat ttgacgttga gctttcgaca agaccggaag aaaattatgt cggaacgatt
    37021 caaaattggg atttggcaga agccggatta aaagaagcca tggataagcg caaaatgaaa
    37081 tatgagatta acgaaggcga cggagctttc tacggtccta aaattgattt taaagttaaa
    37141 gacgcaatcg gaagaacatg gcagtgtgct acaatccagc ttgattttaa tttgcccgaa
    37201 agatttgagc ttaaatatca ggacaaagac ggtcagctga aaacccccgt catgcttcac
    37261 cgagtaattt ttggctcaat ggaaagattt cacggacttt tgattgagca ctacgccggt
    37321 gctttcccca catggcttgc gccgacacag gtttgtcttg ttcctataag caacgaaaaa
    37381 catgccgatt ttgcacaaaa aatctatgaa aaattaaaca atgaaggtat aagagttaag
    37441 cttgatgacc gctctgagag tatgaattac aaaataagag aagcacttca agataagaaa
    37501 attccttatg tggccgttat cggagataaa gaaatcgaag caaattcgct tgcaatccgc
    37561 gccagaggca aaggtcaaat cggagttttt gaaatcgacg attttgtcaa aaaactttcg
    37621 gaagaaatta aatcaaaagg caagaaaatt gtagaataaa ataaagacaa gaaacaaaag
    37681 ggcggatttt tcgaagaaaa atccgccctt ttgtttcttt tgtttaatta cttaacatcg
    37741 tctttgataa taatcagatt attgattatt ttcattgcgc aggtaggaaa aacaacttca
    37801 ttcaagccgt ttgctatgct tattatgctg cctgctttaa gcaccacgtc ttcatttttg
    37861 tattgaaaag tgtaatcatt ttttctgtct gatcttatcg tgattttatt atccatattc
    37921 actccaattt ctaatcgaat acgtagctta tgatagaggc ttctcttgca cgtttaactg
    37981 cttttgcaac cattctttgg tgctcggcgc aatttcccgt aattctgcgg ggaataattt
    38041 ttcctgcttc attcaaatat ctttttaatt ttgaagcatc tttgtagtcg ataaaatcga
    38101 ttttatccgc gcaaaagctg cagtttttgt gcttttttaa gtcttttaaa ttttctcttg
    38161 ccatttgttt gtttgctcct tgtttttgtt tattacttta tgttttgatg aagagtgagc
    38221 tgtgagctgt ggtgaggatt ttaaattctg ttcaccgttc accgttcact gttcacttta
    38281 gaaaggaatt tcgtcttcgc cgattaattc atctgagaat tcttcttggg cgaatttgac
    38341 tatttcctcg ttattactgt ttttgacggg tgctgttgcg cccatttttt caacggtgtt
    38401 ggcatctatt tcatagattt ttctctcgga accgtcgtcc atttttgccg ttgcgacttc
    38461 aagtctgcct tctaccaaaa tcctgtcgcc tttatttatt gatgaagcaa cttcatctaa
    38521 ggacattctt ttgcacataa cccttataag cgtttcttct ttttcgccta tgtcaagtac
    38581 aaatgcccaa atagcaacat tatttgtggt aaaacgtttt tcgggtgttc tgtaaacagt
    38641 ccctgttaca actgcttttg caatagtcat aaatcacatc cttttagtat tttagattgc
    38701 cacggggttt tcaacccctc gcaatgacac acttttaaga ttcggctttg cctcatcaag
    38761 aattatgtaa cttgtgtttt tgccatttcg acaaacataa ttctcaaaac gttttcgtta
    38821 agcttaagtt gtcttttaaa ttcctctact ttatcagccg gaattgaaag aacctgcgtt
    38881 gtaaaaaatc cgtctctgaa tttttggata tcgtaggcaa gtttttttct gccgatttta
    38941 tcggtgcttg aaacttttcc accgaagctt tctattgttt cttggatttt tgcaataact
    39001 ttatcaactt catccaagtc taaattcggt ttaattaccg ttaacaattc ataatttttc
    39061 aatttttctg tctcctttat atttggctat agtataaaaa tcttatcata aacaaaaatt
    39121 ttacaacaga tattcggcta gaaaactgta ttgagcaggt ccgctcatga aaacatcaaa
    39181 atcggtattg tctgaaaaac cttgccactc aatggttaga gccccgccaa gaagattaac
    39241 ttttactttg ccttctgtta agttatttaa aacagcagcc acaactgttg cacaagcacc
    39301 tgttccgcag gctaaagtta tcccgcatcc gcgttcaaaa actcttaaat cgatttcatt
    39361 ttttgaaata atctttgcaa attccacatt ggttttttcg gggaaaagtt cgtgcttttc
    39421 aatttctggt ccgtaagttt ttgccaactc caaaaggtct tcatctgtgt caacaaaaat
    39481 tacacaatgg ggattaccca ttgatacaat gtttgtttca aaaattctgc cttttatttt
    39541 gagattattt ttagtgcatt cacctcttgg ttttgacatg ttaactttga cttgcccgtc
    39601 ttcaagaatt tcggggatga ttattcctgc aagcgtgctt acgctgaatt ttttggaatt
    39661 gaccagtttt ctgtcataaa cgtattttgc aaaacatctt atgccgtttc cgcacatttg
    39721 tgcggtagag ccgtcggagt tgtagaagaa ccaacctatg tctgcatctt ttgtgcaaag
    39781 agcaggaata atcatgccgt ctgcgcctat gccgaaattt ctgtcacaga gtttttttgc
    39841 caactcatcc ataggtaaac ccgatttttc gtattcgcag taatccacga ttacaaaatc
    39901 atttccaagc ccttgcattt tggttacgtt aattttattc ataaaataat tttagcataa
    39961 aaacgattgt taaacagcct gtcttgcaca ttctctgtca attaaataag gctttccttt
    40021 gtaaagtcca atctgatcaa acctgtttgt ttgaagatct cttggggtgt agcctgaaaa
    40081 ttttatttta tttattacat caataacatg ctcggtggtt aagtttgttc tgtcggcttt
    40141 cggttgaatg tagtaatata catcgccaat ctttccgctg tctaagaccg gcaaatcaaa
    40201 caatgagttg tgaggtcttg tggtaagatt gttgcttaaa gcgattttaa ggacttcttg
    40261 attgtccaat tcaaacgctg cggtttttga acctactcca ataattcctt ttatttttct
    40321 tcctttataa agtcctgatt tttccaattc ttgtatgaag ctttcaacca aaccttcggg
    40381 aatgttgtga atatggtttt tttgtttaat ttctgttatt tctttaaggc cttttagtaa
    40441 tttggggggc tttttaaaat ttactaaatc ttgtgcacat cccattttac taaaaaaagc
    40501 ttccaaattt ccgaacgttt ttttaaataa atcatctgaa attttaacga ttttaggagg
    40561 tttattttta gagaaaatat ttcttacaaa atttagaaca gattttaaat tgaaattttt
    40621 aataacgggt tgagggcttg ttttattgcc tatagatatt aaatctgcgc taacttcaga
    40681 taaaatcggt gctttttttg acacagcagg caaggctttt ctaagaccgg ttgaacctat
    40741 ttttaatacg ccgtttataa ttcccattta acaccaaaaa ttctacctta tagatataaa
    40801 gtaaaaattt ataaaaaaat tgcttaaaat ttagtatgaa tttttattga gtgcctttat
    40861 ctgctttgcc tctttacttt ttagtgtggt tatgataaaa atattgtgtt gccgaaaaat
    40921 cgcggaggag tgccagagcg gtttatagga gcggtcttga aaaccgtcgt acgtgacgag
    40981 cgtaccgtgg gttcgaatcc cacctcctcc tagttaaaga gcccaccttt aggtgggctc
    41041 tttaactagg agggtgagaa cccaaacgaa gcgaagcttt gtcagtgggt tcgagcacta
    41101 cgaaaaacaa ggagaggaac gagccgtagt tttacggatg caaccagcta aatttcaagg
    41161 acaaagtccg tagaaatttg cgtgttgcca cctccttttg atttaagttg tcaaaaacag
    41221 agtaggtccg attcaaatta gacttaaaac attatatcta taagtaatat tcccataaga
    41281 tgtgttatgc aaatatttat ttccaagctt taatatgtgc tccggcaaat gccccgtcta
    41341 tttcttcaag attgattttt gcaagttcgt ttttgtcttt gactaactct tcaataatag
    41401 cttttgaata aatatgcatc ggctctattt caatattttt aaatccgaca tctgacaata
    41461 tttgtttata ttcattaatt aaaatcgttc ctgcaataca acctgcccat aactctgcct
    41521 gctttttaag ctcgggcgta acttctctta aagatacaac atcagctacc gctaatcttc
    41581 caccttcttt tataaccctg taggcttccg ataatgcttt tttcttgtct gaactaaggt
    41641 taataacaca gtttgaaatg accgcatcta ctgaattgtc ggctaaagga atattttcta
    41701 tatagccttt gataaattca acattatctg ctcccatttt ttctttattt ttattggcaa
    41761 gttcaagcat ttgatcagtc atatcaagcc cgtaaatttt acctgacttc ccgacatatt
    41821 tggaagacat caagacatca attcctccgc cgctgcccaa atccaagacg gtttcgcctt
    41881 cttttaactc ggcaagtacc aaggggttgg agcaacccaa agacgcgtta atagcctcta
    41941 tcggtaaatc gtctaattct gccccgtcat atattacaga agttttgctt atttcgtcgc
    42001 aacaggcaca actgcttgta tttcctttgc ttacatcact tgcaatatca ccgtaaaatt
    42061 cttttacttt ttctctaata tcaaattcca tatttaaact cctttatact tccaaaaatt
    42121 tacttacttg actatatgac aaatttatca tatagtcaag tgttttaaca gcgcaaatga
    42181 aatttaattt cctgaatttt cttcaaacat tttgttcatg caaacaaaaa attcgcccat
    42241 acaacatatt tttaagctgt aatacacatt cattccgtct tttctgtctt ttacaatccc
    42301 gttggatttt aaaaccgcaa gacattttga aacatttgat tgttcctcac ccgttaattc
    42361 gataatttca gatacatttt tttcaccgct tgcaagcaaa tccacaatca gtaatcttgt
    42421 cggatttgcc agtgctttaa tcatttttgt tttcttttta tatagttctt tattcatttt
    42481 atgccctgaa atattaaaca gttgactata tgacaattat atcatatagt gttattatgc
    42541 ttctgacagg aggataataa catgaaaaaa atcgaaattc taggaggcgg ttgccaaaaa
    42601 tgcaatgatt tggcagaaaa agtcaaagca gtggcagact caatggggat tgaatacgaa
    42661 ctggaaaaag ttacagatat tacaaaaatc ataaattacg gagtgataaa aacacccgca
    42721 cttgtagttg acggggaagt taaagtttca ggtgaaatcc cttctgtaga tgagataaag
    42781 gagttgctat gaaaaaatta ttattaattc taacaattct tttaggattt tcaaatactg
    42841 tttttgcagc tgataaagta aatgtttact atttttacgg aaagccaaga tgtgtaactt
    42901 gccaaaagat tgaaaactat actaaacaag ccgttgaatc aatgaaagac aaagatgtta
    42961 attacaaaag cattgatatg gaaaagtccg aaaacagttc aatggttaaa aaatataatt
    43021 tattcaccaa atctgttgtt atttcaaaga ctaaaaacgg caaagaacaa tggaagaatc
    43081 ttgacaaggt ttggttgaaa gtcggaaacg aacaagattt caaaaattat ataattacgg
    43141 aaacaaaaat ttttaagggc tcaaaataat ggttatagca atactttcag cactatggtt
    43201 aggaatactg acagctatca gcccgtgtcc tttggcgaca aatatagctg caatttcttt
    43261 tatatcaaaa aatattgaat gcccttacag aacagtctgg gcaggatttt tgtacacatt
    43321 aggaagagtt ttggcttatg taattttagg ttttattgtt gtaggcggac ttttggctgt
    43381 tccgagcgta gcaaactttc ttcaacattc aataactaaa tttatcggtc ctttgttaat
    43441 tttaataggt ttacttttgt tgaatgtaat taaactaaat ttttcttttg gagtaaaaca
    43501 cgaaaaaatc caagagtttg ccgcaacttc aaatttcttg ggagcatttt tacttggtgt
    43561 aatttttgca ctgtcttttt gccctgtatc tgcggcttta tttttcggaa gtcttacttc
    43621 tttagcggtt gaatatcatt caagattaat aatcccgaca atgtacggaa taggaacagg
    43681 tttgcctgtc gtaatttttg caatagtagt agcattttca atacataaat tgggagagat
    43741 ttataacaat atggttaaat tcgagttttg gtttaggaga ataacaggcg gaatttttat
    43801 aattgcaggt gtgtatttta tagtaattca ttttatgtag agaataggag aacgattatg
    43861 acagaagaac tacccggatt tataatgtgt atttgtaccg gcaaatgccc cggatttcag
    43921 gcaatggaca tatgggattt cattaatcag gtcagggttg aattacccgt tgaatacgct
    43981 tttatccacc cgcaactttg cgaagaagac ggcgacagat ttttggcgga ttttttaaaa
    44041 gccaacagaa aagttgttat agctgcctgt gctccaaaca tgcaaagaaa aatgtttaag
    44101 gatgctttca aagaggcagg gcttgacttg gaaaaagatg ctgtaatgct tgatattcgt
    44161 gatatgacca accaacaggc aatgaaaaaa gtagcaaaag cactaagcga tatgggctta
    44221 gatgcagatg aggaggatta ctaatgagta ataatgatga ggatatgaag atacttccac
    44281 ccacgggcga tacttttatg ggcgtcccga gagacaaaat cgactggtgt ccgacgattg
    44341 accccaaaaa atgtaacgat tgtatggaat gtttaaaatt ttgtcctcac aaagtttttg
    44401 atgttgatga aaacgcaaaa cctaaactta tagtaaaaaa cccgaaaaac tgcgttgttt
    44461 tttgtcgcgc gtgcagtaag acttgcggac ctgatgcaat atcttttcca agcaagcctg
    44521 aaacaatcaa aaaaataaaa caaactcgaa aaggacttaa tcaaattgag taatttgatt
    44581 tttcgcctcg ccccttgagg gagaggccga ctttcagtac gagcaatagc ttgcgagtat
    44641 tagaaagtcg ggtgagaggt actacaaccg tcattcgctt tttacccctc accctgaatt
    44701 tcagacactc atacttcgtg ccaaaattca tccctctccc ccaaggggcg agggtaaagc
    44761 aaaaaggaga aacttaatga ataaaaaata cgcaatattg ccttgtaacg ggctggataa
    44821 atcagcaggt tgtgtagccc gtgaaatagc acttcaaata gcagaacaaa caggaagcga
    44881 gattatttgc cctgttttat atcaggtcgc tgatgcccgt tataaaaaaa taacacaaga
    44941 taatccgctg ttagtaattg acggttgtca gactcgttgc gcgagtaaac ttgctgcgga
    45001 taaaggatta aaagttacta aaaaaataac ggttacagaa tatgcaaaaa ataacgggat
    45061 tgaattatgc gaaccattaa gaattgacga tgcggcaatt aaccttgcaa aaactcttac
    45121 aaaagatgct ctcaaagaaa cttcatcagc tgaaaataaa ggtgaaactt tcgcaatgcc
    45181 ggaagatatt caatattcaa cctaccaaaa agacaagttt attttcaaaa ccccaacaga
    45241 aggatttttg tttaatgaaa atgattgttg ggcatacgtt ataggcaatg ttgcaagagt
    45301 cggtgttgcc gattatgttc agcacagcct ttcggacatt atctttttca ccccgccgaa
    45361 tgtaggtaac gaagtagaac agtttgaaga actggggcaa attgaatcag gaaaggcggt
    45421 ttttgaagtg atttctcctg taagcgggaa aataataaga gttaacgaag cgttaataaa
    45481 tgcacccgag ttaatcaacc aagacccata tgttaaagga tggattgcag atatagagct
    45541 ttcaaatttt gaagaagaca aagaattttt actcgattat gagggctatt ttagtgtttt
    45601 aaaaagaaag gtggatgaat atcatgtcaa aaaataaggt taaaataatc ccttgcagcg
    45661 ggatgggcaa agcattcgga cttatcgcaa gagaaagcac tcttgagtca ataaaaaaat
    45721 gtccccaaga tgccgaaaca gtttgtctgg catacgttgt gacaggtgat gaagaagcaa
    45781 aaaaacttgt tgaaggtcaa aagtgtatta caattgacgg ctgccctgca atgtgttctg
    45841 caaaaaacat tgaaatagca ggaggaatta tagaagaaaa aatcagagtt gttgatgttt
    45901 tcagaaatca cagaggtgta aacgcaggca gcgcgacaga actgacagaa gacggctgga
    45961 aaatagttga tgaaatcgcc gaagatgtgg ctaagaaaat taagggggct aaataatgaa
    46021 taaaaataat acaaaaatag gtgttgtttc ttgcagcgga gaagactgcc tcggcggaac
    46081 catatcaaga ctggcaacca gaaaaatgct tgaactcaga gccgacgaaa cggttacaat
    46141 ttgcctcccg ttgtatttag taggcggaga agaggaaaga ggatttgcaa acgaatatcc
    46201 tacaatttct gttgacggat gcgataaata ttgcggtaaa cgtgctacag aaaaatacag
    46261 cggaaaagta agcgcatcaa taaatgtcgg tgatattatc ggagaagaaa ctgccaaatc
    46321 agcagcactt tcttgcaggg atttgacaga tgttcataaa gctatggtta aaaaagtttc
    46381 cgatgttatt caagaaaaat atgatgaaat cttgaaaaag tgaaaggtga aaggtgagag
    46441 gtgagaagaa aactgttcaa ctattcgacc tttcaactat tcaactaatt aatgttcttt
    46501 atccaaaatg agaatacaaa tccatatttt aatctggctc ttgaagaata tcttttaaaa
    46561 gaattcgacc aggattgctt tatgctgtgg caaaacgaac cctgcgttgt tgttggtaaa
    46621 aatcagaacg ttttaaatga aattaatgaa gattttatca gaaaaaataa tatcaaggtt
    46681 gcgcgcagaa tctcaggggg cggggcggtt tatcacgatt tagggaattt aaatttcact
    46741 tttatcataa acgatacaag aaatgatttg cgaaattatc gtaaatttac aactctgatt
    46801 attgaggttt tggcaaaact tgggataaaa gcagaattat ccgagcgaaa tgatttaaca
    46861 attgagggtt taaaattttc gggcaatgca cagtataaaa acaaaaacag gttgctgcac
    46921 cacggcacaa ttcttttttc gtcgaattta gataatgtta aaactgcact aaaagtcggt
    46981 aaaaacaaga gcggaagatg ggttaaatct tttgcaagcc cgataacaaa cattcaagac
    47041 catattagaa cacatttaag tatcaatgag ttaaaaacgt ttattcaaaa tcactttgaa
    47101 ggcaaccaaa aaaattatca catttataac ttaactttga cagacatcga aaaagtaaac
    47161 aaacttgtta aacaaaagta tgctaccgat aaatggattt tcaaagactc tttaattgag
    47221 ttatattctt gaaataaaaa agtataattc tgccttttga ggattttagt gggatgagaa
    47281 accatacaag cacttaggag ttacttactt gttaatatct caagtgcctt ttttacaatt
    47341 ttgtgtactt tttgttgtat ttaaattctt ttttccatag gcttattatg tatcaaatac
    47401 taaacctttg ggcgattaaa ttattacaga tataatctaa aataatcttg cccggaggat
    47461 ctcaggatta taagacccgc cgggctcttt gtttaaagtt aaagctaagc agttcttagg
    47521 caagagcata accagtttgt acatgttttt ataaattttt ctttttgctg ataaattaaa
    47581 tcttcggcat ggttacagcc ttcaaaaagt tcaaattctt ttttgcattt tgctttttca
    47641 aacaaacttt ttgtatcgtc gggaagtatt attgtgtcgt tatctccggc tataaaaagt
    47701 gttgggcagt ttattttatc cacaacatca attggtttga ccattttttg tataatcgga
    47761 cttaccctca ctctaatcca ttttttaaat tcatattttt taaaagggtt tgtcaaaaaa
    47821 tcttttatcc acttaacgtt tttgattttt tcaaagctgt gcggagcaga aacgattata
    47881 agacaatcag cgtttgattg tgcggcactt tgaataattg atatcgcccc tcccatggaa
    47941 aaacctatca cataaacttt tttgtaaata ccttttgcat aatcgacaac agcatttaag
    48001 tcatcaggtt cttttttgct gaaagtatag aatcccgaac ttctgccatg ccctctgaaa
    48061 tcaaaagaaa ttacgtcaaa agtctttgca aattcaatgg cggcttgctt aatgaatttg
    48121 ctatctttgc tcattgtcca gcccggtgca atgagcaaaa cctgttcttt atttcttttg
    48181 taatggttga gtgcaagttt aactttagct tttaaataaa tttctgaata ttgtttcata
    48241 aataaattct caacaatatt attgtttatt ttttaatttt ttcaatttga ttaatcattt
    48301 ttgaaatttg tgcggcttct cattttgtca acttaaatct tcagaagttc cttcacgtct
    48361 tttcttttaa cttttcttgt tgtagttttt ggcaaaggtt cgttgtgaac tattatattt
    48421 ataggccttt tgtaagaagc aagctggttt gaaaggtatt tgacttcttc tcttactatt
    48481 ctgttgattt ctttttcgtc tttgacttta ttaatcaagt cttcttttgg cacaacaacg
    48541 gctacaatat cttctgttcc gtcttttgac ccgccggatc ttgcggagcc gagaacgcaa
    48601 acttctgcaa aatattggct tttttccaaa accgattcca cttcttcggg gaatactttt
    48661 ttgccgccgg ataaaacaat catattctta agtcttcctg ttataaaaat gtgactatcc
    48721 tcatctattc ttcctatatc tccggtatga agccacccgt cggcatcaat tacttcggcg
    48781 gttaattccg gttgattatg ataccctttc atgactgaag gtcctcttaa aaggatttcg
    48841 cctgttttag ggtcaatttt tgcttcaaaa ctttccaaag gtctgcctac agaagctaac
    48901 atccagcgtt tgtcatagtt tacagagaca acggggctgg tttctgaaag cccgtaacct
    48961 tgataaactt ttattcctat tgtttcaaaa aattcggcaa cggctaaatc aagtggggca
    49021 cctcctgcga tacatccgtt aaaattaccg ccgaatttgt cgtgaatttt tttgaacatc
    49081 atttttctca ctgcataaga aggaatcctt tttgcaatgt tatacattac tttaaaacct
    49141 acttggacag atttgggcga gtttgcaatc tctgtttcaa ttcctgcttt aagtaatttc
    49201 aagaacgcag ggacaactat cataaagtta atcccctttt ctctcattat gcttaaaata
    49261 tctttcggct taaggctttg ggtgtaataa accgacaacc cccagtaaag gaatgtagaa
    49321 aatcccaccg taagttcaaa taaatgattc ataggcaaaa ttgaaagtac ggaaatgccc
    49381 tcgtttttat caaaaaagtg ataataaact ttgctcatgt cgtttaattg agcaagcatg
    49441 tttctgaaag ttatttcaac gccctttgga gcacctgttg ttcctgaagt ataaattata
    49501 aaagctgttg attttgagct tctgtgccgc cacttgcagt cataattgtt tggcaacgtg
    49561 taaagacaag gcatttgaag gttataagac ggttcatcaa ttaaaattac atgcttaatt
    49621 gatttaattt ctttttgaag gtcaagtgct ttatctatat tttcttgtga agtcaataaa
    49681 atagtgggct gacaatctaa aaggatagat tttaattcat atttggtaag ttttacatct
    49741 aaaggaacgg ttaccatacc ggacattaca gaggcaaaaa cgcaggctcc gtattcgggt
    49801 tttgattcgg ataaaatagc cagtttctcg ccttttttaa cttgaatgtc gtttataaga
    49861 taagaggcaa gttttcttga taaaagtcct atccctttaa aggtaaattc cttccatcct
    49921 aaagcggttt taatccctaa ggcaaccctg tcattataaa catcggtttt ttcttccaat
    49981 aaaagaagaa tattactctt tttatacttc atcaaatcct ccaacagccc ccattccaac
    50041 actaaaagac catctattat ttaatcctat aaataaagtt ctatgatgtc aagtaatttg
    50101 taatttttgt ttagttgaag ttttctacgc caaatgcatg atttttaact aaattaagga
    50161 ctttttcgga caaaaatgtc gtaatattaa ttataaaatt gtgcaaaatt gacttttgca
    50221 caaaagttat taaataaaaa aacgtaaaaa caaaggagaa tgacactatg ggtatggcag
    50281 catcacaggc aaggtactta ggcttaaccg ccagaaaaac caatacggaa tacgaaggtc
    50341 aacagataaa ccaacagcgt acagctcttg caaaccagag tgcgggtttg tttaacagaa
    50401 tgttgtccct ggaagttccg acacctccaa gcactcaaga ttatacaaaa gtaagctata
    50461 cttatagcga tggcgtgaat aaagaaacca taagcagctt tacaccgctt gcaggggacc
    50521 ctgattacaa ttataccgtg acccatttcc ataattctca agtttataca ggtatagaga
    50581 caaaacaggt taatccgcag gttagaaaaa gcggttcaac ttatacttat atcggaaatt
    50641 cacctttaac tttggtcagc gtggcaaaac aacaagcagg tgtaacagaa cctcaaataa
    50701 tatatagtgg tgatggagct acaagaactt atacatcact tacagagggt ggctcttcca
    50761 ggtcgttaag tgcatacacg actgcagata gagcagcctt agagcaatta gcggcacttc
    50821 atcctacgtc aaatctcgga cagacatttt tagacggtga tactagcgaa atatacaggt
    50881 acaccggctc tgacggcgaa gtgcattatg tctgcaaaga tgacttggat gcatcaatag
    50941 gttcaacagc atcaggaggt gccggaatgg ctgctcttga taattacacc ggtggtgata
    51001 ctacgagttg ggcagctgta aaccaaattg ttacagacaa cgcttcttcg caatttgcag
    51061 aagatgcaat tgcaggtgat gatatttatt cttatcagct gggcggagta acttattatg
    51121 tttgcgaatc tgatttagac gcaagctata acagtataat tccaaacggt gcatttgaag
    51181 gtcaaagtgc tttgaatcgt tataattcgg catatcttga cacaagagtg gaaacaacgg
    51241 aaaaagcatt tttgcagcag gatcaatcag gcagatttat atctgctaag cttgaaaact
    51301 catctccgac ctttactctc ggttcaagta ccgtaaccga cgataacgct tacaatgacg
    51361 caatgaatca gtataattac aatactttta aatatcaaaa agaaattgaa gatataaacg
    51421 ctaaaacaaa aattatacaa gaagaggaca gaacccttga actgcgattg aaacagcttg
    51481 acactgagca aaaagcactt caaaccgaaa tggaagcggt caaaaaagtt atcgacaaaa
    51541 acgttgaaat gacctttaaa acattttctt cttaaaaaat ttaatttttt gcttaaattt
    51601 tttgctcaaa aactccttca aacggatgat ttttactccg aagaaggaat tttcgggttt
    51661 aaaatgtcgt ataattatta gtagttaaat aaaggctttt aggagattct tgactttttg
    51721 aaaagaatca ttaataaggt tatatacaaa ttaattagat aggagaataa aactatgggt
    51781 atggcagcat cacaggcaag gtacttaggc ttaaccgcca gaaaaaccaa tacggaatac
    51841 gaaggtcaac agataaacca acagcgtaca gctcttgcaa accagagtgc ggggcttttt
    51901 aaccaaatgt tatccctgca ggttccgaca cctccgagca ctcaggatta tactacagta
    51961 agctacactt atagcgatgg cgttaacaat caaaccataa gcaatttttc accgcttgca
    52021 ggggatcctg actacaatta caccgtaacc catttttaca actcaaatat ttatacaggc
    52081 atagaaacaa aacaggttaa tccgcaggtt agaaaaagcg gttcgactta tacttatgtc
    52141 ggaaattcac ctttaacttt ggtcagcgtg gcaaaacaac aagcagatgt aacagatcct
    52201 aaaatagtat atagtggttt agtaggcgta agaacataca catcacttac tgacggtaca
    52261 acttcttgta ctttaagtgc atacagttcg actgcagata gagcagcctt agagcaatta
    52321 gctgcacttc atcctacgtc aaatcttgcc acagcatttt taagcggcga tactgacgag
    52381 atatacagtt acacaggtga agacggcaaa gtgcattacg tatgcaaaac cgatttagat
    52441 acatcaatag gcggaatatc atcaggaggt gccggaatgg ctgatcttga taattacacc
    52501 ggcggcgatg ctacaaactg ggcagcaata aaccaagtta ttacagacaa cgcttcttcg
    52561 caattcaaaa cggatgcagt cgcaggtgat gatatttatt cttatcagct tggcggagta
    52621 acttattatg tttgcgaatc tgatttagat gcaagctata acagcagcgg cggagcgata
    52681 gaagggcaaa tagcacttaa cagatacaat tcggcatatc ttgacacaaa agtggaaaca
    52741 accgaaagag cgtttcttca acaggatcaa tcgggcagat ttatttccgc aaaacttgaa
    52801 aactcatctc cgacattttc tctgaattca gctaccataa ctgatgaaaa tgcttacaat
    52861 gatgcgatga atcagtataa ttacgataca atgaaatatc agcagcaagt gcaggatata
    52921 aacgcaaaaa cacagattat tcaagagcag gacagaaccc ttgaactgcg tttaaaacag
    52981 cttgatacag aacaacaagc acttcaaacc gaaatggaag cagttaaaaa agttatcgac
    53041 aaaaacgtcg aaacaacatt caaaacattc ggcgggtaag taattaaata aaatcttttt
    53101 actactcaat tatcaaaaaa catctctaaa ataccccaca aaggaaaaac aaaatgtctt
    53161 acagatacga tagttacctt gtaatagcaa acaaattcag ctcggcaggt tccgatgcca
    53221 aagcgcagct ttttgaaact tacgacaggt tgcgtgattt gactgtttcg ggttcggctg
    53281 caacgggcac aggatttgaa caagcgggcg gatttttata tcaggttgcc agtttatttt
    53341 cgccttcatc atttatgaca acaatgggcg gaacttctgc aatgaatatt cctgggactt
    53401 cttactggtc acctgtaagc ggcggaaacg ctgtcgttaa cggtggttat tctgcctttg
    53461 ggataggcgg attgggtaat tattcaggat ttcccggagg tgctgcaaac ataaatttgg
    53521 ggctcggtga ttataccggc ggggcatctt caattttgtc gggatttggt tcaatgcaag
    53581 gttctgcaac aggatttgct tcgagcatag gcggaatagc cgatgtggca agtgcagcag
    53641 cttacggcgc aggtatggga agcggttacg gatttgggac aaacgttgtt cttcctattg
    53701 caggtgttat ctcgggttgg ggcggcatta tgcaggcagc ggcaccttat cttggagaat
    53761 acggacttcc ggcaattgtt atgggtaatt tgatgcaggg aacaacttct gcggcacttt
    53821 ctgcttatca gaacgtttcc ggcaggattc tttccaatgc ggattctatt ttgacaaata
    53881 aagtaagaaa tattgaaaca gtttgtaaaa tgcttgatac tcaaggcgat gtcgttagaa
    53941 aaatgctcaa agaaagcatt gactctgaca gcaaagctat tcaaaattta tagaaaattt
    54001 aggtttttaa atgttgattc gaacatatat acgcacactc ttcaataaaa tttttaccca
    54061 aaactttatt gttaaatttt tgggtaaaat tgtaaacttt tattgcgaag tttggtttaa
    54121 aacgtcaaaa aacataaagt ctgaacaaaa tttgccgatt aacatgacaa aggaacaaat
    54181 gttgttcttt caaagtgtta caggcgacaa ggtggactgt aaatagttaa gacatgttaa
    54241 gtgtgttgtt tacatgaggc aataattcaa tcttaaatgt ttttattatt atgaaggtcc
    54301 ggtaaagggt atcaaagtgt atcaagaaac tttacagaca aacttcaaaa gatttatgaa
    54361 ttttttagga ttcgcaaggt cattttttct cagaagaaat cctataaaga ctttcataaa
    54421 tggtttagca gaagccttaa aagacttttt gacaatgaac aaaaaactta aattggctcg
    54481 atataggcta agttatcaca gacatcagct aaacaaaatg gaatctctca tagcagaaaa
    54541 taatcaacat actttcctta gaggaaataa aattaacgaa gtcagataaa ggtagaaaaa
    54601 aatgggttta ttaatagcaa cattaagaaa aacgtattta atagcttata aaaatgagtt
    54661 agaatataag attcagctta taacccaagc taaaatcgca ttgactcagt caactaacga
    54721 tttgttgaat gtcggtacag acatggatcc tgacaatccg gtcgtaaaac aattggagca
    54781 gcgcaaagaa agacttaatc tgcttgagaa aaaacttgat atgcaaatgg atgaatataa
    54841 aacaaaactt gatatggttg aaaaagaaat acaaagttgt gacggaatgt ttaaaagcaa
    54901 ccttgaatcg tccttctctt atggcggcgg acgttaacag ataaaaattg aaacaaagcg
    54961 gcgggtttga aaaaacccgc cgctttgttt ttcagccttt taattattaa gttgtttttg
    55021 tttgcaatta taattttttt tggtgtataa ttgaatgata taagaggatg cgggaaaatt
    55081 cgaaaaatga caatttttcg caatttttca aatccgacct tagaagtgct aaatccttgc
    55141 cggctgcggg atagccggcg gcaaggatga aagccggaca agagaaaagg gcgtgaaaat
    55201 gaaagttgat ttaaaaaaag aaaaagacaa tgttgtgaca ttagacattc aggttcctgc
    55261 aaaagatgcg gaaaatgaat acaataaagc agtcagaaaa gtttctgagt atgtaaacat
    55321 tcccggtttc agaaaaggca aggctcctag aaacattgtt gaaaagcatg tcggaataga
    55381 aagaattcag caggaagcgt tggagcgtat gttgccggct attttcaaag atgcaataac
    55441 aaaaaacgat ttagacattg tatctcaacc ttatgttgaa tcttatgatt ttaatttggg
    55501 agaggattta aaaatagttg caaaagttga gcttcgtccg gagataaaac ttggtgaata
    55561 taaggggtta aagcttgagg cacagggtta tacaattcct gaagacgctt ttgacaaagc
    55621 attagaaggg cttttgcagc agcatgttaa gtatgaattg gttgtaggca gacctgctca
    55681 aaaaacagat attgtggtaa ttgattttga cggttcggtg aatggagaaa aaataaaagg
    55741 cggagcggcg gaaaattatc ctcttgattt ggcaaactct aatttcatac ccggctttgc
    55801 agagcaactt ataggtaaaa atttgggtga agaatttgat attgaagtaa aatttcccga
    55861 agaatatcac gaagcaaaac tcgcaggaca gcctgcaatt tttaagataa aaattaaaga
    55921 aataaaagaa aaagttttac ctgaattgac agatgaattt gcgcaaaaac tgggaacttt
    55981 taagaccgtt gaagatttaa aggcagatat tcaaaaatat cttgaagcaa caaaaaacaa
    56041 tgaagatgca aaaatcataa gcaacactat ttttgaaaaa gtgttgtcag gtgttaaaat
    56101 tgatattcaa aattcaatgg tggaaagaga aactcaatct ttgcttgaag aatacaaaca
    56161 aaggcttatg atgcagggaa tcgaatggca aaaagcagtt gaatctcaag gtgaagaaaa
    56221 tatcttaaaa ggcttaaaag aagaagcttt gcttaggatt aaaaattcac tggtaataga
    56281 cgaaattgca aaagttgaaa atataaaaat aggtcctgaa gaattggaca aaaaattaaa
    56341 agaaattgaa gatgcctaca aaatgccaag ggcggagctt atgaagcaat taagccaaaa
    56401 tccgcaggta ttcagttctt tgtctcagca ggctctaaac gaaaaagtcg caaagttttt
    56461 gaacgaaaac aatacggttg aattggctgc tgataaagag aaaaaggcca aatctgcgaa
    56521 gaaaaatgac taaaaagcgt agatttattg tgaaaatatg ttataatata tttggataag
    56581 gaaaggaaaa gaagatgagt tttgtacctg tagtaataga gcagtcaagc agaggtgagc
    56641 gttcttttga cattttttca aggttgctca gagaaagaat tatatttttg ggaactccgg
    56701 ttgatgatat ggtggcaaat ttaattgttg cccaaatgct tcttttagac agtgaaaatc
    56761 cggaaaaaga tattatgctc tacataaaca gccccggcgg aagcgttact gcaggatttg
    56821 cgatatacga tactatgcag cacataagag ccgatgtaag cacaatttgc ttaggtcagg
    56881 cggcaagtat gggagcattc cttttggcgg caggcacaaa aggtaaaagg atggcacttc
    56941 ctcattcgcg cgtacttatc caccaacctt tgggcggagc ccaaggtcag gctactgata
    57001 ttgaaattca ggcggcagaa atcgtaagaa ttaaaaaatc gttgaatgaa attcttgctg
    57061 caaacacagg acaatcaatt aaaaagatag aaaaagatac agacagagat tatattatga
    57121 cccccgaaga agctttagaa tacggaatga ttgacaaaat tgtctcaaaa gaaattctaa
    57181 aggagtccaa ataggagaaa acctatgtcg aaacatgacg atagcagatt aaaatgttcg
    57241 ttttgcggaa aaacacagga tcaggtaaaa aaattaatag caggtcctga agtttatata
    57301 tgtgatgaat gtgttgaact ttgcaacgaa attcttgatg aggaattttt tgaaggcaaa
    57361 gataaagatg ctgcaggcga aaaaggaaaa gaggcagaag aaaaaactat tccaaaacct
    57421 gcggagataa aatcttacct tgatgagcat attgtaggtc aagaagatgc gaaaaaagtt
    57481 ttgtctgttg cggtttacaa tcactataaa agattaaggc acaacaaatc aaaagataaa
    57541 gaaggcggag ttgaaatcca aaaatcaaat atcctacttg tcggtcctac aggaagcgga
    57601 aaaactcttc ttgcacaaac acttgcaaaa atgcttgatg ttccttttgc ggttgccgat
    57661 gccacaactt taaccgaagc cggctatgtg ggcgatgatg ttgaaaacat tttgctcaga
    57721 ctgcttcaaa atgccgaata tgatgcggca aaagcagagc ggggcataat ttatatcgac
    57781 gaaatagata aaatcgccag aaaatccgaa aatacttcaa ttacaagaga tgtttcaggt
    57841 gaaggggttc agcaggcgtt attgaaaatg attgaaggta ctgttgcaaa tgttcctcct
    57901 caaggcggaa gaaaacaccc tcatcaggaa tttatccaga ttgatacaag caacattttg
    57961 tttattgtgg gcggtgcttt tgtaggattg gaaaaaatcg ttgaaagacg tgcagacgat
    58021 ggaagttcaa taggctttgg cgcaaaaaca gcttcctcta aagcagaaaa agaggcgcag
    58081 gctgcaaaaa tgctcaaaaa acttcagccc gaagacttgc ttaaattcgg attaatccct
    58141 gagtttatcg gcagagttcc tgtttatgcg gttttagatg ctttggatga aaaaactttg
    58201 aaaatgattt taacagagcc taaaaatgct attttgaaac agtacaaatg tcttttaggt
    58261 atggacggcg ttgaacttga atttgagcct gaagctattg atttaatagc agctgacgca
    58321 ataaaaagaa aaacaggcgc aagggcactt cgttctatag ttgaagaaat tatgcttgat
    58381 attatgtatg atattccttc caaagagcac atggacaaat ttgttataac agcagatatg
    58441 gttaaaaaca ggaataaagc tgaattaatc aagcttccta caaagaaaaa acttgaacaa
    58501 gaagaagaaa tagcttagtt tttgtttatt taagtattaa ttttggactt tgctaaagtc
    58561 cttcgcgtca ttctgagggc ggagcccgaa gaatctctta tcacgtcgtt gcgaggggga
    58621 acaatatatt tacccccgaa gcaatctaaa atgttataat tttttgaata ttttttggga
    58681 ccttttggtg gcaaaaggtc ccgccaaaag ccaccccctg ctcgtcgctt tttgccaaag
    58741 tacggctcat tccattgaaa atttcaaact cgctttgctc aaacagtgaa attttcgggc
    58801 attacatttc acctactctg gcataaaagc tcctcaaagg gggattttta tctatgcttg
    58861 ctccgatatg gtcggaaaag agtccgtaca gggtttcaga gtgaagcact gacgctttga
    58921 caaagcgaac aggcgaagaa tgagccttgt gagcctgtct ctgtaagtga gcatttcgtt
    58981 cgcgtgtttt ccccttttct tgccttcttt tgcctgaaac ttgtttcagt gcaaaatggc
    59041 gtcacgaaga gtgactactg cctggtttct tttctttttt tcaatcgcaa gaaaaaaaga
    59101 aaagaaatga ggcatcaaaa attggagggg atatttttct aatttaaaaa attttagatt
    59161 gcaaaaactc aatttctatt acatgactag caattttttg taacatatgt tataattata
    59221 ataacaagag gatagaaaaa tgatttcttc caaggtaaaa gttcttgtta ttatttccgc
    59281 attattatta tccgtaggtc ttatgtctgt tggctgcaaa tcggcagctc aaaagggttg
    59341 ggtaaatact gattctatgc ccggacacac tttcagcaag cacaaaaaca agaacaatca
    59401 aaacactcaa attgtttatt taggggcttc cgaaaaagat tcttctacgg cagttgattt
    59461 tggcccccaa gatgtcagtt ttgagttttc aaatttgaag taatttttag ttgaacagtc
    59521 gaatagttta tgagcttgta aattcttctt actatttact atttacgtat taccccttga
    59581 aaaatgtttt ttttgtatta ctatactgtt agtagtggtc aaaaaataag gggttattat
    59641 gataggcgga gttttagaaa ttgagattat gaactgcatt tggtcgttgc aaaaacagga
    59701 tgaagatgcc aatatttctg ttgcggatgt cgtagctttt ttaagcagaa ataaggtgaa
    59761 aagagcttac actacgatta aaaccgttat ggacagatta agtgccaaag gcttgcttgc
    59821 cagatataaa agcggcaaaa aattctttta cagaagcaca ttagatagag aagaagcggc
    59881 aaaagatgca ctaaaagaag tttcaacaca attttttggc ggaaattatg ttgaaatgat
    59941 taaatttata gagaaggaat gttcggccgt tttggtttaa gggagaattt gtgaatattc
    60001 aagatgacag gaaatcggtt tcaaggctta tttataaagt tttgacagag tcattgactg
    60061 ttcgtgaggc gattttgaat ttccccaaag atgttaatga tgcttcatta aaaacagcct
    60121 atcatgcgct ggttcatctt gaggcggatg aagatttgag aagaagggat ttgctttaca
    60181 ggcaagaaca ggatgattat ttggagttta tagcccaaac ccttcaaaaa ggagaagttt
    60241 taccttataa tattattaaa gagtatgaaa agtattatga aggaatttct acccctcatt
    60301 caagaagtat gaaaggtata ttaaagagtt tatgcaaatt tttaaacgtt tagcatttaa
    60361 aaaacccgcg cagaacattg ttgaatttat ttttataatg cctgttttag ttatttttac
    60421 gcttgctata tttgaacttg cactatattg gcaggatgtg aacgcggttt attctct
//
LOCUS       DAOQAU010000006        59843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_374_length_59843_cov_5.943132, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000006 DAOQAU010000000
VERSION     DAOQAU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 59843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59843
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_59843_cov_5.943132"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="fusA"
                     /locus_tag="PKI94_01890"
     CDS             <1..404
                     /gene="fusA"
                     /locus_tag="PKI94_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNW25521.1"
                     /translation="YDGTYHDVDSSEMAFKIAGSMAMKEGTKKAKPCLLEPMMKVEIE
                     VPEENTGDVIGDISSRRGRIEGMEMIEGTGIQKINVVVPLAEMFGYSTDIRSKTQGRG
                     TFSMEFKCYEQVPSNIETEIIAKAGGNKDKD"
     gene            complement(490..1032)
                     /locus_tag="PKI94_01895"
     CDS             complement(490..1032)
                     /locus_tag="PKI94_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNW25522.1"
                     /translation="MKSSTLRRSNLSKERMAMLEELAKNRQKPYDASPGVYVRASKEK
                     ELDVLWQNFRVSQKEDKSPGIYLATGFIVGAVAMLIMTTLLSFSVRRDDTQDIKPPKR
                     MKAEKARLTFIPSDKNNETATSTKNETYTVKDGDTLESIIVQFYGKFDPSKVEKIRVA
                     NKMVNPNKLQIGQNLIVPLD"
     gene            1277..2935
                     /locus_tag="PKI94_01900"
     CDS             1277..2935
                     /locus_tag="PKI94_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25523.1"
                     /translation="MLEQAIMGSNLSINLQKARESLEIAREHHEKYLVRLNNQDLQFA
                     IEHYITAIKLDPSIPETYYRLASLMWENGQICLNTAIEQCKTAVSIAPNNINAHIYTG
                     YFLKLAHEFNSAENEFKNAIKLGGVNSARPRLILSLALLEKMNTQKTSVREFAKCLYY
                     LTTGTLMMAWDLASLKMLYKNFAEDFSIFSYKNAGEFCEKFNRLNAAAKVYEKGAEIT
                     GCEELFYHKTADICVKKREMESAVENYQKVLDANPKNRDVLIKLATIIQTFFPDDSDI
                     AIDCYNKLLEIEEDNAKIYYELGHLYLNKNDKIHALSAFKLALDKDSENPFYNNALAY
                     AFVQAELYDEAIEHYQKAIKLNPDKQWTAIVCQALGAIYYQIKENFEAAVASFQAGIV
                     LDPDNSEIYLSLGDVYMAEDDLDKAIRAYCDSISINPEDYRAYSKAGLALWEKDYLEE
                     AIVAYHRAIDLNPDYDIAHNNLGVIYLDGIGTPDEALTYFQNAIEINPNYTLAYFNAG
                     RALQALAKNNDAADYYQMAMDLNKLTQDIDEEDIKERLYGLFNI"
     gene            3004..4146
                     /gene="dxr"
                     /locus_tag="PKI94_01905"
     CDS             3004..4146
                     /gene="dxr"
                     /locus_tag="PKI94_01905"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNW25524.1"
                     /translation="MKKKISIIGSTGSIGTQTLEVIEKLQDKFEIIALAGGDNVKLLR
                     EQILKFKPKKVCVKSLSARNELKAEFSKLEIFCDDEGLDEICSDRSNDLIVVAVSGKI
                     GLKPTLKAIENKIDIALANKETLVMAGDIVMKKAIENNVKILPIDSEHSALHQCIKDI
                     SMVEKLIITASGGPFLYKSLEEIKNATVEQTLAHPKWKMGKKITVDSATLMNKGLEVI
                     EAHHLFGFDYDKIEVIIHPQSLIHSAVEYVDGSVVAQIGLPSMHIPIQYAITYPERFE
                     GIKSKSFSFLDAKRFDFEKPDLEKFPCLKMAYEAGKMGGSATVCLNAANEEAVFSFLE
                     GKIKLYRISEIVEKILSEHKLVKIPSIEEIFEIDKEVRIKTREFRS"
     gene            complement(4136..5305)
                     /locus_tag="PKI94_01910"
     CDS             complement(4136..5305)
                     /locus_tag="PKI94_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25525.1"
                     /translation="MYKKILTILFLGVFLSANCLIAAIAEPELFKGRAEKSEQIKEQS
                     ELFTGKIDTLDSKDVIRMTVSQVLDCSFSQEGDEFFAEVTNEVSGPTGVIIPMGTVAH
                     GKISESKGAKRLGRDGSLTLDFDYLITPDGREIPIEGKMTTKASSAKAVGKIVATDIG
                     YTAVGGVVGGIVALNFFGLEAAIASQGYTVAGGAAVGGAIGLGASLYRKGKDVLISPG
                     DEIRVKINTSIPLPVYKDTALMQHEMFCEGLDVKINNITYENDPFGEANTITLSLVIT
                     NRTKMTFSSFDLALVNDYNSVFHPSIFGDTKLMFTEIKPGDRVAGRVSFSVDNVKRKF
                     WLTFYDRRSRKAIAKISIDNAYRKVSNETKKKNERFFIKKKSNYYKDNDPFEANF"
     gene            5489..5728
                     /locus_tag="PKI94_01915"
     CDS             5489..5728
                     /locus_tag="PKI94_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25526.1"
                     /translation="MAIGARDYIDQLLVKKYAQEKVDNHDNIASMVSPEKMMEAMNDY
                     AAMHRNMFALSQKLTSACYAINARSAKYNKAVNFS"
     gene            5804..6367
                     /locus_tag="PKI94_01920"
     CDS             5804..6367
                     /locus_tag="PKI94_01920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNW25527.1"
                     /translation="MKKIILASASPRRKEILQNAGFDFEIKPSDFDEKLDDLDFSYEK
                     IEKLAYEKAFSVAKKLSDKKYVVGADTVVVFANNILGKPKNTQEAINMLKMLSGNVHC
                     VVTSICVIESATMEFKIASVTTYVEFNKLSDIEISNYVNKFMPLDKAGAYGIQELPKN
                     FVKKIDGSFENVVGLCPKSVQDLLKEF"
     gene            6545..7369
                     /locus_tag="PKI94_01925"
     CDS             6545..7369
                     /locus_tag="PKI94_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25528.1"
                     /translation="MGMSASQARLLTITSRMSDLELQAQNITNNKIRLSMKSQEIAQN
                     YSDALNNKKLVFNKTDISVTANNLTSYNKDSLDVQRILKDSSGRVVVSQSIIDAFNVD
                     PNHVSSFCHNLQPPVKISADDEAVPGEFDLAKFKHYFNLWSQLRDCGYNIQSDANLNN
                     SDWLQEQLSNGNLMIAQMKSNAWYGANGAPFTHSVFDTISYSSDTSLVEELDESDDAA
                     AEAEYKVKTAELQAKDKIMDNELNQIQTEHKALETEYDSVKKVIEKNIEKTFKIFG"
     gene            complement(7366..9060)
                     /locus_tag="PKI94_01930"
     CDS             complement(7366..9060)
                     /locus_tag="PKI94_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNW25529.1"
                     /translation="MKVVDYIAKYIENLGIEQVFGYQGAAINPLIDAIVKTGKVKYIQ
                     AYHEQAAGFCADAYSRVTGKTGIALVTNGPGVTNLISAIANAHLDSVPCLFFTGQVNT
                     FDINPSFLNIRQHSFQEVDTINMVKPVTKYAKTITDALKIKYELEKALYIANEGRKGT
                     VLIDLPFDIQNEEIEEKYLDNFKTKEKSQTDDKILQKINQAAEMISKAKRPVVIVGGG
                     LRSAEALNEFEKFIEITNMPSVSTLMGLDIFSKTNVGFSGLYGTTPANLTVYNADLMI
                     FLGSRFSKRHFGFNRDHFNINGKIIHIDIDKHELNRATKEDLSINLDAKAFLIELNKQ
                     LNTLKMKDLSQWEKIIQKWQKKYENNTQINKNGLDPVKFISEISKYLPEDAIITTDVG
                     QNQMWVAQGLKVKKNQRILTSGGLACMGFSLPAAIGAKLAQPNKNVFAFSGDGGFQMN
                     LQELQMISDKKINIKCFVFNNSTLGMIKELQHKFFGSRYNGTGNEYGVPDLKQIAKIY
                     SMDYLKINKNYKIEALKQKLESKNPCLIEVELNKDNTFVLNKNCEPEIYKENLVKN"
     gene            complement(9073..10137)
                     /locus_tag="PKI94_01935"
     CDS             complement(9073..10137)
                     /locus_tag="PKI94_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNW25530.1"
                     /translation="MQKNEKIYLAGHSGLVGSAIMRKLKSEGFNNIITKELHELDLCN
                     QKAVEEFFEKEKPDYVFLAAAKVGGIMANSTYPAEFIYQNIMIASNVIHASYKFGVKK
                     LLNLGSSCIYPKLAPQPMKEDCLLTSELESTNEAYAIAKIAAIKLCRYYNEQYGTNFI
                     SVMPTNQYGAGDNFNMETAHLLPMLLRRFHLAKLLKNGDFEAIKADLKKYKLGWGLDD
                     KLNLNDKTSITKALEGIGAYSDKVVVWGDGSPYRELMHSDDLGDACFYLMQNKNYNDI
                     GELVNITDGTDIQLKDLINTVKEIVGFEGKIVYDKSKPNGTPRKLMDATKIKSLGWSP
                     KINLKEGIKAAYQWYLSSTN"
     gene            complement(10139..11230)
                     /gene="gmd"
                     /locus_tag="PKI94_01940"
     CDS             complement(10139..11230)
                     /gene="gmd"
                     /locus_tag="PKI94_01940"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNW25531.1"
                     /translation="MAKVALITGITGQDGSYLAEFLLKKGYEVHGMKRRSSSFNTARI
                     DHLYQDVHNKNLKFKLHYGDLTDSTNLIRLIQEIQPDEIYNLAAQSHVKVSFDCPEYT
                     ANADGLGTLRILEAIRILKLEKKVKFYQASTSELYGLVQETPQKETTPFYPRSPYACA
                     KLYGYWISVNYREGYNIFACNGILFNHESPRRGETFVTRKITRAAVRIKLGIEEKLFL
                     GNLDSKRDWGHAKDYVEGMWLVLQQDKPEDYVLATGVTTTIRDFCNMAFREVGIDLEW
                     KGRGVGEKGIDKATGKTVIEVDPRYFRPTEVDLLLGDATKAQRRLGWKPKYDLKTLIE
                     EMVAEDLKEAKKEVFLKQGGYEVLNPQEV"
     gene            complement(11298..11924)
                     /locus_tag="PKI94_01945"
     CDS             complement(11298..11924)
                     /locus_tag="PKI94_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNW25532.1"
                     /translation="MSALDSILAPVVHFIHTTISTMGPLGVSMLMAIESCNVPLPSEA
                     ILPFAGYLVSKGNMTIHTAAFAGALGCVLGSIPSYYLGYFGGRPFVEKYGKWFLISHR
                     DLEMADKWEQKYGNLAFFICRMLPIVRTFISLPAGILKADIKMFTLFTFLGSLIWSYP
                     LVYVGIKLGEHTEALKAFWHKFDLAIVLVLGTLFVLYLINHFKHLKES"
     gene            12037..13191
                     /locus_tag="PKI94_01950"
     CDS             12037..13191
                     /locus_tag="PKI94_01950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNW25533.1"
                     /translation="MLNFEFCCPTKVVFGKGSIAKLSELIDNDKKILMIYGGGSIMKN
                     GVYNQVKVALNDFNMIEFGGIEPNPTYETCMEAVKIVKKEKINFLLAVGGGSVLDATK
                     FIAAAAKYKGEEPYDIISKCAPFDEFIPFGSVITLPATGSEMNANTVISRKSTQEKLA
                     LQSGKLYPTFSIIDPEVTFTLPTKQTVNGIVDTFVHVMEQYATYDVNSPLQDSWALGL
                     IKTLINESPKVLTNPKDYEARANIFWCATCGLNFWLSCGLVQDWATHRIGHELTALYG
                     LDHGQSLAIILPRLWQYKKKDKAKKLAKMARDVYGCVEKDELKAADYTIKKTEEFFNS
                     IGMKTKLSDYGINPQEAAKIVKERLEQRKVVFGENNDITPQVAYEVLLKC"
     gene            complement(13251..13544)
                     /locus_tag="PKI94_01955"
     CDS             complement(13251..13544)
                     /locus_tag="PKI94_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25534.1"
                     /translation="MGCDISHEEKLEHMQHYFFKAFIISFVLLLFSSLMCIFFFDYQA
                     QMMEKLFQLDVDDYSMLLVFLMGLWKILIIQFTLVPALAIWCMRKCCCCKGKK"
     gene            13836..14912
                     /locus_tag="PKI94_01960"
     CDS             13836..14912
                     /locus_tag="PKI94_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25535.1"
                     /translation="MYKFKKFVKILFSKVFLLIVFAAFLLSIFFYWGWYERQYDKGLG
                     MYYVYKGDKAYQQRDLQKAIGYYNTALEVFPSHYGARLNLGNIFVVYEDYYAAADSYE
                     KAIEYNKNFSEARMNLGIVLSEKIGDFDSAISQYQAIINSKPRVFSIPFIFDNKKSER
                     INRGLAYYNMGIAYRQKALYLEDDQDKEEKAAMFLRKAIGAYKNAVRILKSDYDSRYN
                     LALAYHLVGNYQKAGLTYCEAIDIEPMNYEAHYNLAILLRHLRMYQEAKEELEKASIL
                     ISSTHSDSYTSRYIFDVLNEVTHNALYRTEYEHLREKIDDEPSYAQKVAYVHGKIVAS
                     DELDKAMIKNFRNCRTKKLFKDYE"
     gene            14916..16367
                     /locus_tag="PKI94_01965"
     CDS             14916..16367
                     /locus_tag="PKI94_01965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNW25536.1"
                     /translation="MKNNNFELNLLAEISKVLTENSNPSDIVLLLSKIFSDFADLSKL
                     SIFIYDENTKLLKDYSKSWVVIDEFHKNFYTDKLYIALANFSQWDFYINDKKFKTLDF
                     KFSGEIKLTKDINKILIPLKRKNKPYGIVELSFSGDISKLLTYEFFIALSVVSYQLSL
                     KIQNTILAEQMQTNIDFHDAMKNIAKIIETQYELNYIIPLIGEMIDRFISEHLVYIFL
                     KNEKNGKIELFWPRACRDKKILSAVSKVNTKSKITLLDDEKIGIFPLIGEKTLLGCIV
                     AHSNVDKLTKNEIEYLEQLSKQSSITIHRAEVYAEVLQHATLDALTGLNNRRQFEMRL
                     KQEYSTAKRQHKPLCAMMIDVDFFKKVNDTYGHNAGDCVLKNVANLIKAGLRDYDIAS
                     RYGGEEFAVLLPYTKIEEAFAVAQRLRQSIEKAKIDISGEGDVDVKPISVTISIGLYQ
                     FKSEDSLEILYQNADKALYDAKTHGRNKVVIFK"
     gene            complement(16580..17590)
                     /locus_tag="PKI94_01970"
     CDS             complement(16580..17590)
                     /locus_tag="PKI94_01970"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNW25537.1"
                     /translation="MVLEMNFPQLEKAIRPTHDIKLFSGRSNYTLTHEIANYLGTTVG
                     PMVIKNFADGEIYVQVKESVRGDDVFIVQPLCNPVNENLMELLIIIDAFKRASAKTIT
                     AVIPYYGYARQDRKTSGREAITAKLVADLLTTAGADRVLAMDLHTGQIQGFFNILVDH
                     IFATPILVDYIKKLNLTSDELVAVSPDTGGVPRTRYFAKELDCPLAIIDKRRDKHNEA
                     VAAHVIGDVKDKICIMFDDIIDTAGTICEAAKLLKKEGAKDVYVCAAHAVFSGPAVER
                     LTNSPITEVIITNTIPVDMSNMPAKIKQLSVAPLLGEAISRIHDDESVSSLFGFEKEL
                     KE"
     gene            complement(17679..18392)
                     /locus_tag="PKI94_01975"
     CDS             complement(17679..18392)
                     /locus_tag="PKI94_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNW25538.1"
                     /translation="MKKIFFAFLTVLLISVVAIAAEPQKEEGYISTSFSQTKEIPPNI
                     AEISIAVETSAQTLQKASDDNKAICEKVYSGLKSIINTASGDYIKTKNYSARPVYVYT
                     KENKKVFDKYIVSNTISVKTKNVDLVSKIIDKSIELGATNVNNLEFFAADYDSACNDL
                     LAELTKKAYTQASSVATTMNSKVLGVKSINTTCSTQNNPAPYYTLMAKSTQDTAASTP
                     IEGGKIKIYANVDASFYVK"
     gene            complement(18413..18823)
                     /locus_tag="PKI94_01980"
     CDS             complement(18413..18823)
                     /locus_tag="PKI94_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25539.1"
                     /translation="MKKNLKIIQINGFRGILLGLFIVSCLIAGFIAFPAFLTMNGWNY
                     LSSTTGSFPPINFTQGVLLWGIIVLSIYIFNKKKFIVSFNARQELSDEELSEVISKIR
                     AQTIKNGLLQDRDKNIENKNQTEEDLELHSENKE"
     gene            complement(18836..20893)
                     /gene="ligA"
                     /locus_tag="PKI94_01985"
     CDS             complement(18836..20893)
                     /gene="ligA"
                     /locus_tag="PKI94_01985"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNW25540.1"
                     /translation="MNILDRINFLKEQINRHNYLYYVEENPSLSDFEYDEMFRELQKL
                     ESENPLLITPDSPTQRLGGISSSFEPHKHKYRLYSLDNSNNYEELIKWYERITKEVGE
                     DVELVCELKIDGLAISLTYENGIFTTGATRGDGEVGENITNNLKTIKAIPLKLNPSPQ
                     PSPARGEGETPEPSHHLSLLTSHFSLEVRGEIYMPKSSFEKLNEENLENGEKIFANPR
                     NAASGSVRQLDSSITAKRDLSMLCYSAILTDTNLKPKTHYENLMLLKKLGFKISPNTQ
                     AVKNIQEAIEYCKNWETKRFELDYATDGVVIKVNDLRLQEELGFTSRAPKWATAFKFP
                     PEEVNTKLLDIETNVGKTGAVTPVAILEPVQLAGSTVGRASLHNFDEIKRLEIRIGDT
                     VLIKKAAEIIPKVIKVSQHAPNSSQYTPQNDCPVCHTPLETREGEVNLYCPNSLFCPA
                     QIKAKIEYWVSKEAMDIDFVGPSIIEQLFDKNMVKSAADLYALSQQDFMQLDLVQDKS
                     AFNIYNSIQASKNPTLTRFLTALSIRHVGKETAHIIAQHFQKLENVSKANLEELAQIE
                     GIGEKIAKNVFDFFRNEKSLEFLNRLQELGIIPKSEASNKISNILAGKTFVLTGTLQT
                     MTRDEAEEKIKSLGGKTSSSVSKNTSFVVAGESAGSKLTKAQNLGVIILSEHEFLDMI
                     NKV"
     gene            complement(20914..21147)
                     /locus_tag="PKI94_01990"
     CDS             complement(20914..21147)
                     /locus_tag="PKI94_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25541.1"
                     /translation="MTTNDELIKALPNARKQKIIENSDKEIVKWGGKRRGAGRKPVNG
                     VVLEFRINVSQQEKEFINYAREHKIDFKELMQG"
     gene            complement(21134..21460)
                     /locus_tag="PKI94_01995"
     CDS             complement(21134..21460)
                     /locus_tag="PKI94_01995"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNW25542.1"
                     /translation="MKKFNSQYTKEALEQFKELDDNSQDKILEAVRIFEYIGTNYKNI
                     NSLGNGLFELKPKGIRAYFMYAKNKIIIIGLIVLKKTQKAPKRFIEQAMRNIENYKKE
                     FSNDNK"
     gene            21767..22378
                     /locus_tag="PKI94_02000"
     CDS             21767..22378
                     /locus_tag="PKI94_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25543.1"
                     /translation="MNKLKILGEKLNKILCYDYTKTLSVLSSIMLILSLFTLSSVYYD
                     FLILIIFVSSAFILFRYYQSKEKSVFVIFFLLVLLLFNPFIPVIIDRQIWQILNVFSA
                     LIFWIYFLKDFSEKIKIIAILSLILFLTLFTPLPPQEINHLKILGNNGFVVKIKEDSM
                     FAEEKPIMHETIIHNNLITFFIQKCINLIPVKSDEEIKFNFKE"
     gene            complement(22443..23276)
                     /gene="speB"
                     /locus_tag="PKI94_02005"
     CDS             complement(22443..23276)
                     /gene="speB"
                     /locus_tag="PKI94_02005"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNW25544.1"
                     /translation="MLGPFLDRNWMEQNPDYKSSDVVMLGLPFDGTVTYRSGSRFAPE
                     MIRLASWGIETYSPYFDMDLEEVNFHDVGDLEFPLGNTQKSLNLIKENVEQIYKDGKK
                     FFGIGGEHLVTLPAIQAISKFYKDITVVHFDAHTDLRKEYLGEEMSHSAVMYHIGKIV
                     GFKNLKQIGIRSGTKEEFDLMDKHHTRLNFPEQISPLVNKNVFVSVDLDVLDPSIMSG
                     TGTPEAGGLTFNELIDWFKKLKGLNIVGADVVELAPHYDASGVSTAVAAKVIRELLMC
                     M"
     gene            complement(23350..23487)
                     /locus_tag="PKI94_02010"
     CDS             complement(23350..23487)
                     /locus_tag="PKI94_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25545.1"
                     /translation="MFDLENEAIIIEIRELVNGIDKVKEEVDVYCDLVKQMFLSKSGM
                     N"
     gene            23658..24920
                     /gene="thiC"
                     /locus_tag="PKI94_02015"
     CDS             23658..24920
                     /gene="thiC"
                     /locus_tag="PKI94_02015"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNW25546.1"
                     /translation="MTTQIIEAQNGRITPEMFAVAEKEGYKQEEILEKVASGRVVILK
                     NNRRENVVPTAVGEGMTVKINANIGTSLERSSIEEELDKVKIIEATGADVLMDLSTGD
                     KIDETRKAIIAATNLPIGTVPMYQAGKEALDLQKDISKLSKEKLFSDIEKHCKDGVDF
                     ITVHCGVTKFVVNQLEKQGRITNIVSRGGSMLACWIKATGMENPLYEYYDELLDLVRE
                     YDCTLSLGDGLRPGCTADAGDRAQVAETIVLGELVQRARKAGVQTMVEGPGHVPLNKI
                     PSMIETIKVLTDYAPLYVLGPLVCDVAPGYDHITSAIGGTLAAYHGADFLCYVTPAEH
                     LGLPDVKQVREGIIASKIAAHAADLARGNKFAIERNLKMSVARKDLDWDGQRKYSIDK
                     CVFEKIDQGKPCTMCGQYCSMKIFKEYF"
     gene            25015..26130
                     /locus_tag="PKI94_02020"
     CDS             25015..26130
                     /locus_tag="PKI94_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW25547.1"
                     /translation="MSINFVDKTIASDLEYILLSSERGKKHKVLAVDDEENNLALIKR
                     TLRGEYEVIIAYNGQEALEIIDKMGDEISLIVSDQRMPMVSGTELFKKVSEKYPDIIN
                     ILLTGHSNIDILLDSVNECQLFQYILKPFDPEHLCMVIQSGIRKFEMTTSRSQILCDL
                     SELFYKTIKSIAFALDAKDKYTHGHSMRVTLYSLALAKNLELPDDLLEEIETAGLLHD
                     IGKIAIPEKILLKPGKLTDEEFEIIKSHPELGEKLVKSVEKLGLISNWLKHHHERYDG
                     KGYPDGLVGEDIPISARIIALADTYDAMTSDRAYRKALSHEEAIAEVERCSGTQFDPN
                     LAKIFIEIQEEIKHLKENSEQEYAKYSYLNKFMNSKS"
     gene            complement(26125..26946)
                     /locus_tag="PKI94_02025"
     CDS             complement(26125..26946)
                     /locus_tag="PKI94_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25548.1"
                     /translation="MRKLIWLFSALLLVFLTTQTCFAQDKKILILPDNIQFESTNYYI
                     YPDTSVIFASDTINSLRLSGKVDTVSMAEIRDAFRKNVRLNITTRRALKEYKYNYNIA
                     FVDIKPIAHTFDTNKVLLITSTTDTQSYLLRRTVWDFLNIPGAAVIDPAYRVSTYIAL
                     VDVDEEKVLWQGTFQKNLSAVENRMIGVNFAPATKQLEKLKFYSSYFTPMIAQIVENK
                     LCPSNIPVKSVESKIIDENNLAPESVKIEEIDIGNKPFAPTKPRDKKSNFMINDL"
     gene            27051..27434
                     /gene="cdd"
                     /locus_tag="PKI94_02030"
     CDS             27051..27434
                     /gene="cdd"
                     /locus_tag="PKI94_02030"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNW25549.1"
                     /translation="MDYSQLLQKAKDASNKAYCPYSHFPVGACVLTKSGKTYSGCNVE
                     NASYGLSVCAERNAIANAAIAGDLDIVAIAVYAQKMENCLPCGACRQIIFEFQKDNEI
                     DVIIQFGEGYKIYKINYLLPEAFSL"
     gene            complement(27635..28255)
                     /locus_tag="PKI94_02035"
     CDS             complement(27635..28255)
                     /locus_tag="PKI94_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25550.1"
                     /translation="MKRKAQNIIEISLLACVIAIIGFSVFVIYNNQKISLADKSKSKI
                     FAAQSVNLNDIDQDMNDIIPYNGNANIPPAGLTYLSMTQGKFNLLMSNITYQNLSDAL
                     NLTPATPMPMNLFEYANSALNLDPPLSPSTINSQTISALIDAFNYAIQNPSAAGSSDY
                     LSQFQSLVNSAENTTIEGTAVVTSQGPTGNSCTSQADCGSGEGRID"
     gene            complement(28364..29689)
                     /locus_tag="PKI94_02040"
     CDS             complement(28364..29689)
                     /locus_tag="PKI94_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW25551.1"
                     /translation="MQKTTLIIDKRKELSTKYKKLIENDENIVLISRSLNSAIKLIQD
                     NEPDLIIISDSIEEKLSDFCKKLRILTYNMRPAIVAVSKSAETEDKINALENGADDFL
                     SEPINSEEFKTRIKAHLRREHESNINLKTMLPNKNYSLKALKRIISKDSKWACLLISV
                     QNFENYKEIYSELASDKLIQTYCAIIKSALEKEDYFGHFSENEFLIITHPIRAEKIAS
                     YLTFAFDSISQKFYSTQDVNRGYTILQGDELAGRRSDFVFSTIGITSNEFKKYTDVKQ
                     IINSLLQVHNLAKKPYGSSYAVERPKISAQNAVLDKNFNNKIWVMEEDEALSFLLATN
                     LDLEGYETQLLKNPQAMRTLLSTDITVDTPPAVIIIDAGDIETLSGLNICKYLKENTK
                     FKNSKIIITSVLHDKELVLNTGAELYIPKPYQLSSLMKWVKIFISEFND"
     gene            complement(29839..30582)
                     /locus_tag="PKI94_02045"
     CDS             complement(29839..30582)
                     /locus_tag="PKI94_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW25552.1"
                     /translation="MDENQKQQDFISTVSHELRTPLTSIRGFAQTMLNSWDALDDDNK
                     KKFIEIIEQQSNRLINLVENLLSVNKLQSFKDDFIFKNANVSTLIEPIIQIVKHQYPQ
                     HEFQTDFNPKIPKIFVDVDKFQQIMTNLIENAAKYSNEGTKIIVKADFSPCPDYLSIK
                     VIDEGISINQEDYDKIFTKFSRLDNPLTRKVQGSGLGLYITKVLTEKMNGKINVTGSD
                     EGNVFEILIPTENLENQAGKQFKGCENNK"
     gene            complement(30648..30830)
                     /locus_tag="PKI94_02050"
     CDS             complement(30648..30830)
                     /locus_tag="PKI94_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25553.1"
                     /translation="MSIRIGGFNPAYLPTRKQNPSGGSSSEELPCKDSNKPVTPEDYR
                     RLIAGINIKPGNPRIK"
     gene            complement(31302..31910)
                     /locus_tag="PKI94_02055"
     CDS             complement(31302..31910)
                     /locus_tag="PKI94_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25554.1"
                     /translation="MTDVRMQGFKGIDPNAQGPKKKDEAEKKETAPQVAPERAKVDPS
                     TVDAFMKAQAAALGTVVKPSDKEIGKNLEGSKKEEAFEKFMKKLDEQISLDPKNSKLT
                     RLKDSLLAKGIEGVTIPDTGVMETKSTIIIHNPPWDDIIIDVPEGGTVEISTERNVTP
                     KPLPLEQAISEVDIPEVKGGTTIIVKDKDGHVVKLVWIRSGD"
     gene            complement(32518..33777)
                     /gene="tsaB"
                     /locus_tag="PKI94_02060"
     CDS             complement(32518..33777)
                     /gene="tsaB"
                     /locus_tag="PKI94_02060"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNW25555.1"
                     /translation="MNILAFDTCLDKMYIGGRVKDKIIPSKIVETTEKHYHSAFLIST
                     IKKFLKENNLTPKDIDVIVTNIGPGSFTGIRACTTVARIYAQQLNKKAVGVSSLEILS
                     RINSQTSPLAGEGVNDLTSVTFPSPLAGEGEFQHEPASVRNSGEGYKIAHRNYAPYIK
                     EFARKMRKNATPQETKLWSILRNNKLGFKFRRQFSINNKYIADFVCLEKRMIIEIDGG
                     QHNGSFSDIERTFYIENEDFRIIRFWNNEINENIEGCFEFLHNELNTPHPYPLALQYP
                     SPQPSPARGEGEQLFLQSLPQGERESDYFYNSQRKGRGNKPTLVALDARKETAYVAIY
                     DNKKEILPPQAMFLEDLKEMIKKEEYFVITDDKLQNVLGGISYQKDDYDLGRFLTEIA
                     QEKLNTDFDTDWKKLLPLYIQPPAVHK"
     gene            complement(33774..34334)
                     /gene="tsaE"
                     /locus_tag="PKI94_02065"
     CDS             complement(33774..34334)
                     /gene="tsaE"
                     /locus_tag="PKI94_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNW25556.1"
                     /translation="MEITCKTLEDTEKLAQKFALAVKDKGAFVSLYGQIGAGKTAFVK
                     LVAKALNVKERVTSPSFVILNEYKSGKLPIYHFDLYRLENEGVKTILDELREYSEGKI
                     LTFVEWAEFSQDEIPFNHIKINVEYVDNCNLNSCQLIPPRPLRERVGVRGTVSRNSSE
                     GYKHDARKYSFEAFGQENIKIIEGLK"
     gene            34401..35255
                     /locus_tag="PKI94_02070"
     CDS             34401..35255
                     /locus_tag="PKI94_02070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW25557.1"
                     /translation="MIEEKVCYLCGNKDFEKREGNVRDNPDLKVLECKNCSLVVLSSF
                     DHINDKFYENDGMHGGSPDVQKWLNATKDDDKRRFRLLKNEIKGKKVLDFGCGNGGFL
                     SFAQKSARKAVGVELQKSMQEYYNSQNLEVYESIDQVSEKFDYITVFHVLEHIKNPIV
                     LLEKLSEKLNEGGKIIIEVPNSNDALLTVYKNNAFMNFTYWSCHLFLFNAKNLMNLAQ
                     KAEFNINYIKHVQRYGFANHLGWQIFKKPGGHKKMWYLNIGFLNKFYEKSLAQIEATD
                     TILMSISK"
     gene            35424..37196
                     /gene="aspS"
                     /locus_tag="PKI94_02075"
     CDS             35424..37196
                     /gene="aspS"
                     /locus_tag="PKI94_02075"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNW25558.1"
                     /translation="MITKYKTKNCGELNLKNIDEEITLSGWAACIRDLGGIIFVELRD
                     RSGFFQVVADPQVNPQVHKVFEHLKTETVLTVKGKITRRPEETYNEKYATGQVEMYPT
                     FVEVLSEAKVLPFMLDDENVSEDVRLKYRYLDIRREQTLNNLILRHKIVTATRNYLNG
                     QDFLEIETPILINTTPEGARDYLVPSRVHEGKFYALPQSPQIFKQLLMVGGVEKYYQI
                     AKCFRDEDLRADRQPEFTQIDLEMSFVEQQDIINVVEGILVDAFKAAGVEIKPPFIQM
                     TYKEAVERFGSDRPDTRFGLELFDVTDIMEASTFEAFKGVIAGGGTVRAIKIPGIANY
                     SRKDMDDIRNLAISFGAKGLAWVTYMEDGEVKSPVFKFLTEDQIADIQKRADAQRGDI
                     VFFVADKPKIVFDVLGRFRLYFGKKLEMIDENQHNLLWVVDFPMFEYSEEHQRYVSVH
                     HPFTAPNPEDIDKMESDPANCRSIAYDIVYNGTELGGGSVRIHSGELQERVFKALGFT
                     EEEVKQKFSFMVNAFKYGTPPHAGLAIGLDRLVALLAGTTSIRDVIAFPKNSTAKCLM
                     TDAPTDASIMQLRELHLKSTVVKH"
     gene            37486..37815
                     /locus_tag="PKI94_02080"
     CDS             37486..37815
                     /locus_tag="PKI94_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNW25559.1"
                     /translation="MKEIITFELNNCKVPFEDWLCKLDNTVKRRIAQRLARLEEDDYY
                     GDCKAIDNEIKELRFQFGSGYRIYFHETNNIIILLLCGGDKSTQSKDVQKAKDYLKIW
                     RKQNYGT"
     gene            37805..38113
                     /locus_tag="PKI94_02085"
     CDS             37805..38113
                     /locus_tag="PKI94_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW25560.1"
                     /translation="MERKFKNSISWEEVLIRQLQDEEFARDFLNDSFMEYVSGGNFNM
                     FFRSLERVIKARMSVRQFAKEAEIDRTNLYDLFKGKKKPQLKTVIKILAKLGYTLKIA
                     "
     gene            38118..39017
                     /locus_tag="PKI94_02090"
     CDS             38118..39017
                     /locus_tag="PKI94_02090"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNW25561.1"
                     /translation="MKIFNELNENKGLSLAFGFFDGVHLGHQAVIESAVSYAKENDKK
                     SAIITFKNHPCCFFNHIKPKYITKISDKFLLFEKLGVDYVYCLDFDEHLALMSASDYL
                     KEIIINYFEPCAISTGFNHFFGAEKSGNVKFLAKMQESFAYKYIEVPPVLFNNEVISS
                     TRIRENIAIGNIELVNSMLGFEYSIEETVIKGRQIGGTIGFKTANLVYPKDMLEPAFG
                     VYCVNVDYESKMYKGIANFGLRPTVDNTIHPILEVHILDFDKEIYGEKIRVHFLKKIR
                     DEKKFGSLDELKAQIQKDISALN"
     gene            complement(39009..39635)
                     /gene="nth"
                     /locus_tag="PKI94_02095"
     CDS             complement(39009..39635)
                     /gene="nth"
                     /locus_tag="PKI94_02095"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNW25562.1"
                     /translation="MEIDKIVETLKKAKQPQSEFVKLMDSFNNPFLVLISCILSLRTN
                     DRTTYPATLRMLELGRTPAAFAHISPEVLRKAIYPVGFYKNKAEQIIELSKILVEKYN
                     SKVPDTIEELIKFKGVGRKTANLVLAKGFNKPAICVDVHVHRIFNRLGYFKTKTPEET
                     EFALRKKLPEKYWIEINTLMVTHGQNVCKPINPKCDQCPIEKYCPKLI"
     gene            complement(39625..40668)
                     /gene="lpxK"
                     /locus_tag="PKI94_02100"
     CDS             complement(39625..40668)
                     /gene="lpxK"
                     /locus_tag="PKI94_02100"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNW25563.1"
                     /translation="MGLKTYITKIHYNKQAKGLFFSLLKFFSLFYGFASRLKNSLYEK
                     DKLKPKKVDAYVISVGNLTTGGVGKTPVVAQIADYLIEKGEKVAILSRGYGGKLSGVN
                     VISDGENIYYSARKAGDEPHWLAQNTKAIVITAKNRYKGAKFAIDKFCATKIILDDGF
                     QHRKLHRDLDIVLVDSEMGFGNENLLPAGPLREGTEAFERIDRLVIVSKNIDHTRAEK
                     FARILEKKLNKPTSLCYSEPDFAYNIFEPNEKLENGDRIVAFSAIGQPQQFYNFLKNF
                     DVVNTFNFDDHHKYSKNDVQKMQKKCIDMNINKLVTTEKDATKLDQSMFYLKVFALKL
                     KTNLDVEKLINGN"
     gene            complement(40747..42108)
                     /locus_tag="PKI94_02105"
     CDS             complement(40747..42108)
                     /locus_tag="PKI94_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNW25564.1"
                     /translation="MRFSSSFKVGILALIALFILLFTILWVKGRSFSSAARMEIVFKD
                     VNGMRPGSGVQMMGLRVGQVEEIIPFIDGENSFVKMKFVITEPGISIPKASVFSIQQS
                     GLIGEQFLEITPPKTRTTYFSVYGGSDLLYKNDPVQIKLDNKYYDVGKVKDTQILSAK
                     MVPAIMQEKIKTPYAYKIDYVITLPGLIMPDYMLGSMIKDKGVNKLRLQMIDNTVLPY
                     PHQSSPYTVVEPMRIADFMDLQYKAAESLTETNIKINELLSDNVIADLKQTVSNINAL
                     TAQATTTIAKAEKLVDASKEDLDSMVAMANKVSTSFIKLADNINGVVGDENFKPTLIS
                     TAKSIDKLSQNLNKILDNTDAKQLGADLQSIANNLNDISTFVSTMTKDEKLKKQLSDT
                     ILKVNTAMTDISSAMELVNSMTPENKRKIEQIMEDTSATTHNLRKFSDKLNKRFLLFR
                     LMF"
     gene            complement(42144..42866)
                     /locus_tag="PKI94_02110"
     CDS             complement(42144..42866)
                     /locus_tag="PKI94_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW25565.1"
                     /translation="MSIKVKKLIKIFGNKTVIDNVSFEVADGEVLAIVGFSGSGKSTI
                     LKLICELLKPDSGEIETSMGDVAMVFQYSALFDSLNVFNNIAFALKERKEFRKKFSQR
                     GLEEIVAQKLKMVGLEGIEKKYPSELSGGMQKRVSFARAIVTEPQTILYDEPTSGLDP
                     ISSTLIEDYIVRLKEETHASSIVVTHQMSTIQRTADRIIMLYNGKIVYEGTPKDMLTS
                     DNDYARQFVSASLEGPMQMLAE"
     gene            complement(42898..43710)
                     /locus_tag="PKI94_02115"
     CDS             complement(42898..43710)
                     /locus_tag="PKI94_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW25566.1"
                     /translation="MSSQIVSYFKKSATYQLYRLFTKQVDDFFTTLGEISIKAISTFS
                     YIMKLEIDKNEVIAQSARFGVSSLPITLSIVGMSAIIVAMQVAGEMVKQGAGNYVGML
                     VTMLIVREDGVIMSGFAIISMIGSSLAAEIATMRVTEQIDAMKSLKVDPIRYLFVPRV
                     VAGLLMMPLVVIVASVVGVLGGAVASNLAGGLNYRAYFDSVWYGLYMKDIHVCLAKAV
                     TFGGTIALISCTCGYNAKGGAKGVGIATTKAVVWSFVAIVIWDLLFALAFFF"
     gene            complement(43767..44438)
                     /locus_tag="PKI94_02120"
     CDS             complement(43767..44438)
                     /locus_tag="PKI94_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25567.1"
                     /translation="MCPVHDKYANYLRNHLFDYLASSNYQYYQEGRKSEYEQEEYLKN
                     IEKLTSSIQHNIKSFGKLKNNGYRGSIIDSMKNLETLKKLGVKTAISLYPLYDYEGED
                     KCKKEGIEYFYIEMPPDFNNIDSGYCKKFVKFSELTKQGNFILGCEFGTYKTNFAMTA
                     YKILNPHNPLLTHYDQDYEINYTDWINIVDIYETLSADDKKELGYTSEVEEEIIKSFE
                     LRGIY"
     gene            complement(44656..44895)
                     /locus_tag="PKI94_02125"
     CDS             complement(44656..44895)
                     /locus_tag="PKI94_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25568.1"
                     /translation="MKKDFSLKYKNDKNISPVEIFKKNLLELEILCKQANKENFQIVL
                     HSPDKIFEPQNVYELSVLLAEDMVKKFSNKNEIVR"
     gene            complement(44892..45083)
                     /locus_tag="PKI94_02130"
     CDS             complement(44892..45083)
                     /locus_tag="PKI94_02130"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HNW25569.1"
                     /translation="MECKKEENKINCTCTYSCSKKGICCDCVAYHRQNGEIPGCFFPA
                     SAEKTYDRSIANFIRSYQR"
     gene            complement(45073..45630)
                     /locus_tag="PKI94_02135"
     CDS             complement(45073..45630)
                     /locus_tag="PKI94_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNW25570.1"
                     /translation="MKKDNKILLHSCCAICSAYPISHLRELGYEPVVYFFNPNIFPEC
                     EFNKRLGAQKELCKNLNCELIIESYEPKYHEIISFGLEDEPEKGKRCAKCFEMRLTQS
                     AKKAKELGIKSFTTTIVISPHKSFELISKLGKTISNKYGINYLDIDFKKNDGFLKSNQ
                     KAKELGLYRQNYCGCKYSMGAKNGV"
     gene            complement(45617..46819)
                     /locus_tag="PKI94_02140"
     CDS             complement(45617..46819)
                     /locus_tag="PKI94_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNW25571.1"
                     /translation="MFNINVTVVRKIVIFILAVIGFATTVKLSVIYYDSNFNPYALPS
                     FCSINQFVDCDGVAQTVHSQFLGIPLALWGLLFYIFTIFLLFVNKLKHVKFLHFLKVF
                     KHPMRYICALGLISFLISMILASISIFEIKKICILCVFTYFLNLFIAYAAVGKAGFYH
                     SFKISTVDFIHALRIKKYLISFIVLALIAGGVLAYTATSYVLAPQVKRYKSIKSFADM
                     KTNPFKSGGNTLGDKNAKIIIDVYTDYRCPICYTFNIMVYRMAKELGNIKFIHHNLPL
                     DEECNHYVRQEFHEGACMLAKYSVAAQNQGHLWDFNSELFEKQPKTEDDILKLAKSLG
                     YDTIRLRQDANSKDTSDRIQKDIENSIDLGIIGTPTMVMNGKIMPGIKPYYEFKEMLI
                     KAGAVEKR"
     gene            46931..48205
                     /locus_tag="PKI94_02145"
     CDS             46931..48205
                     /locus_tag="PKI94_02145"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNW25572.1"
                     /translation="MKKIKIGMIGLGTVGSGVFKTLSDFENIEVVKIAVKNLKKQRNL
                     PKLNEAILTDNPYEVVKHPDIDIIVEVVGGIEPAFDLIKTAILNGKHVVTANKELLAK
                     HGEELFNFAEEHNKVVLYEAAIAGGIPIIMPIKTILAGNKITKIAGILNGTTNYILTK
                     MDVQGASYESVLKEAQELGYAETDPTGDVEGFDAAYKLATLATIAFNKRINIKNIYRE
                     GISKIQAEDMIFANDFGYKIKLIALAKLDEKGNADVRVHPMLVGKSKSLAHIDYVTNA
                     VEITGHPVGEIMLSGPGAGEYPTASSVVGDVLAIATEIGKTDYLLPMMRCHHHETANM
                     LNISDTYNKYYLSVNVKNNFGVIAAMGSVFAKNNISISTLLQKGLKSDNTANIIVITE
                     KCLEKDMQNAIKAFEKDTCINKINSLIRVMEG"
     gene            48206..49270
                     /gene="thrC"
                     /locus_tag="PKI94_02150"
     CDS             48206..49270
                     /gene="thrC"
                     /locus_tag="PKI94_02150"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNW25573.1"
                     /translation="MSNHYRGLIDKYKEYLPVSDKTPVITLNEGNTPLIKADNLAKKI
                     GINSEIYLKFEGANPTGSFKDRGMTMAVSKAAEAGSNAIICASTGNTSASAAAYGAKA
                     GMRTFVLIPDGYIALGKLSQAMMYGAEIIAIKGNFDEALEVVIQIADKYPVTLVNSVN
                     PYRIEGQKTGAFEICEALDDAPDYHFIPVGNAGNITAYWKGYKEYFEFKKTTKTPKMM
                     GFEAEGAASIVKGERILKPETIATAIRIGNPASWKQAEDARNESGGVINFVTDDEIIY
                     AYKLIASTEGILAEPASAASVAGVIKAKKLGLIEEGSKIVCILTGNGLKDPDSAIKYS
                     ASQVKKTSSQIGDILKVMNI"
     gene            49280..51565
                     /locus_tag="PKI94_02155"
     CDS             49280..51565
                     /locus_tag="PKI94_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25574.1"
                     /translation="MLGFLFAKKEIIKKDFELNQIYLKLKEIYKFDEISDDRKAFLLD
                     LTEKYQYLPYPHIKALEELSDAEVLFALEVKWGLNGIFKNGEFTFENDKISPLSRNDI
                     KNSNWIKREGHDIKLVNLAGLGNGNKYDNAGKFIDWLRQLLILPAGNISKGIFPTTIY
                     LIPFHPREFGCAYLPTSTDVSPNLEDETLKIELGLDAKAQVQTFITLAQLAGHPVIYD
                     VLPQTGRFSKLVLANPFIARWFDINELLAKLENSADLVAQKLEEKFDKEDIEVIKDIC
                     KQTLKSGAGDLSEHYQGIYDEFEAELIELKKQFSQEMSMKENQIKLQKRAKEIIAGIH
                     EQKSSKNFNEHEITNQADCIKGLMAEGLWPLSGGAWCSAGVPVFDRMSDCGSYPMFLH
                     FDFNGEDVSNFANLDCQTPYYFTFLENGQLNVPVVEFFVNLMKKFQEDYNFDGFRVDH
                     IDHIVDEVSQKNDIPISYRAPKEVLCRLNHAMKQKVPYFATMAEYMLWDNYLKEYHED
                     MCFDLLWGNDIISQSEKTPEKIIEDNQNLAIYNTKNFSIENLSILKTYNNQDGEFRVI
                     DQYPGQLGESGALFKWFKYKFLPGGKYAQRPVLYIDGDESFTQKGIERAIGTEVSMKR
                     EKNYDFFAKFDAINRFSKSQELIFEGEAQIIRQDEDGFVCWLISKEAVKNAFLIVANY
                     FCETEKIAVKDLKDGTHIELREGTEVFDKSLVLPCDYVIKSEFVFDGKDFVEKELGHK
                     TNSLSFNVIKPSEFKIFLLKK"
     gene            complement(51568..52089)
                     /locus_tag="PKI94_02160"
     CDS             complement(51568..52089)
                     /locus_tag="PKI94_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25575.1"
                     /translation="MNVTFRANDLQALISQPQKFSQDGATSTKPVETTPNTEEKPNKH
                     GSTGKGFFWGAIVTLAAAIMIAGKGSPAKGFKLLLGGFEKLAKKSMDESIKLKDEAIA
                     ESIKLKDEAIAEANKLKDEAVKELNKLKNVGKRFSKKVKVENKDTKKSTSYIVQQKAK
                     MRAKRAEKAAKRK"
     gene            complement(52171..53007)
                     /locus_tag="PKI94_02165"
     CDS             complement(52171..53007)
                     /locus_tag="PKI94_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNW25576.1"
                     /translation="MTKYSEDTNNSHLPSPLAGEGRISIRGTSIRNSGEGYNLQTGQL
                     QRYNRNIIIEKVGLEGQNKLLQSKVLICGAGGLGSTVIANLASLGIGKIGIVDNDFLE
                     LSNLNRQYIHKFNNLGGIKVESAKKWINEFNPEIEVKTYPIRLDENNYNDIVSEYNLI
                     IDCFDSFQSKFLLNKIAVKTGKILIHGGVSEFYGQATTIVPQKTACLNCILPQEDSFV
                     TKGVLSPTVSLIASIQAMEAVKIILNLGENLENKLLSYDGLNMKFRTINLSKNPDCKI
                     CS"
     gene            complement(52991..54091)
                     /gene="mnmA"
                     /locus_tag="PKI94_02170"
     CDS             complement(52991..54091)
                     /gene="mnmA"
                     /locus_tag="PKI94_02170"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNW25577.1"
                     /translation="MVNKNSKILVGMSGGVDSSVTALLLKEQGHEVIGVTMSIWSGGK
                     YKGDISSDNSCKNACYSPNEKEDIEEAKKIADKIGIPFYTFDCSKEYEKIVLENFKQE
                     YLSGRTPNPCIRCNSLIKFDVLPFLAKRAGLEFDKFATGHYAQVEFDKKNKRYIIKKG
                     INPKKDQSYFLYKLRQDQLANIIMPLGCYEKDEIRKIAKNNGLKVHDKPDSQDFYDGD
                     YNELLEVKPKTGNIVDKNGKILGKHQGIWNFTIGQRKGIGIAHTSPLYVIELNKEKNE
                     VIVGEIDKTFKKSLLAVDINWVSINELKNKTKATAKVRSAQIPVDVKLIPQGNNILVE
                     FDEMQKSLTKGQSVVFYDGDVLLGGGIIDEVF"
     gene            complement(54134..54343)
                     /gene="thiS"
                     /locus_tag="PKI94_02175"
     CDS             complement(54134..54343)
                     /gene="thiS"
                     /locus_tag="PKI94_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNW25578.1"
                     /translation="MTEINLKINGKDIVLKNSSTIEELIKERQVSGNMFVVEQNLTIV
                     PKEIYSTTPIKENDSIEIVGFFGGG"
     gene            complement(54411..54641)
                     /locus_tag="PKI94_02180"
     CDS             complement(54411..54641)
                     /locus_tag="PKI94_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNW25579.1"
                     /translation="MNNWEIDFTDQNNKPLTQREIEVLELIVKGKSNTEIAKELIVSV
                     HTAKAHVCSILQKMSVDDRVQAAVKAIREGLV"
     gene            55018..55689
                     /locus_tag="PKI94_02185"
     CDS             55018..55689
                     /locus_tag="PKI94_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25580.1"
                     /translation="MGFLLFAVRKLFLRRKSNDLSFRSIMLSQKKQEITEQINSVMQG
                     ISQAKSAAAFTAGQMQSIFAKQQNSEMSSIFEDFNANSKKHEFYEAFNGLTDQKEKTA
                     WLKEHQTALTDLGWSSSDNNLDAWEKVKAELSETDNTRMTALQQAQMEQNTKIAMATQ
                     LTNNVFESMSNASLPYLNAEDSRISLEMANIESQLKAVNGELEGVEKAETEAAKSEAP
                     KFGLG"
     gene            complement(55761..55946)
                     /locus_tag="PKI94_02190"
     CDS             complement(55761..55946)
                     /locus_tag="PKI94_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNW25581.1"
                     /translation="MLVTITNRCTGCGACSAINPEVFEIRNNFATVNPEFVDGNEDDC
                     IDAALACPVNAIKIDEY"
     gene            56111..56674
                     /locus_tag="PKI94_02195"
     CDS             56111..56674
                     /locus_tag="PKI94_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25582.1"
                     /translation="MSYKNLNANSLLTGIYAGLTNTYSILAQSNPNGVTLDSISKSYS
                     NTSNGYSINPSFASYLQTNFSTLDKDKDGILSSSEFSNFTNQLSTQGLTQAQLTQLGT
                     ATGLSSEALSEVLSHFAQVDANKDGKVTSAEISSYQVTSAKEKKMEEFTNRSAGNMSV
                     FYGDDSASAADSSSLLDYRYMSDDETT"
     gene            complement(56861..57592)
                     /locus_tag="PKI94_02200"
     CDS             complement(56861..57592)
                     /locus_tag="PKI94_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW25583.1"
                     /translation="MISFFGLCVQNLITSIKYLFSKQMRFSTMISQAAAISYDSLPIS
                     IIIVFIAAAVIAIQVSKQFLMSGAETYIGGTLTIVILREIAPGFAALAIGARAGTAIS
                     AEIANMQVTSQVDAIKTLKVDPVGYYFTPRLISAGITVPMVVLIAELIGIVGGMFVCF
                     FTINLHPNRYINSVWLWLQMRDVYISLFKAFVFGILIALVCATQGYTTQGGAKDVGIS
                     TTQAAIKSTIYMLIADFMINLVFYL"
     gene            complement(57585..58502)
                     /locus_tag="PKI94_02205"
     CDS             complement(57585..58502)
                     /locus_tag="PKI94_02205"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNW25584.1"
                     /translation="MNDFVYMNGQYVEKEKACIPVMTHGFLYGTSIFEGIRAYYNEKE
                     NQLYAFRVPEHFERLNQSAKIMHMKVENSVQELCEITKKLLNKNGYRQNIYIRPTLYK
                     SAQRIGTSLLNNPDSLLIFTVPMEDYIDTTKGLKVCVSNWRRNSDNALPPRAKIGGAY
                     ANASLISSDSKLAGFDDAIALSETGEVTEGSAMNLFLVFGKKLVTTSTTDNILQGITR
                     NTVIQIAKEVMKLEIEERTIDRTELYLADEAFYCGTGAQVSPIVSIDNRQLGDGKVGT
                     ISSELQKLYFDVVCGKVKEYKKWCMPIYD"
     gene            58637..>59843
                     /locus_tag="PKI94_02210"
     CDS             58637..>59843
                     /locus_tag="PKI94_02210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW25585.1"
                     /translation="MELILNLLYFYVAVYSVYFFVASIKNLSDKKFAIQQKYSGINAQ
                     SELCVIVYSHNNEITLQNLVKQLKSQDYPIDKFSVYVILDNCSDNSEKLFINESFIKV
                     FNIADQDTVGKDQAISILLEKLSTVQNFNAYVFLDASRYIDEHFLSSVNASLTDEPVV
                     SGSTVLVSEYPSFKDKVKISFHKYYTNFIQKSRSILGLASIIDSDILIMRQEIIEKIG
                     CVDFQSINSELKYSLLLSKVGLPCSFNPNIKTYIGVEDFNLRIPSISARLNLFKNCFT
                     QLWTKNFVFIEHVFSLLTPNVLLLTFIYLYLLKHSFKYYFIVDFSVVLLSFIMLILGF
                     SLSLINSKLNREEMLYLFLYPFYSLGHIIKNLKLVKRIKSKITGKDEVFVNTEKLVTD
                     VLVTDGKSNI"
BASE COUNT        18420 a        10031 c        10766 g        20626 t
ORIGIN      
        1 tgtatgacgg aacttaccac gatgttgact cttctgagat ggcgttcaaa atcgcaggtt
       61 ctatggctat gaaagaagga actaaaaaag ctaaaccttg ccttcttgaa cctatgatga
      121 aagttgaaat cgaagtcccc gaagaaaata cgggcgacgt tattggcgat atttcttcaa
      181 gaagaggtcg tattgaaggt atggaaatga ttgaaggaac aggaattcaa aaaattaatg
      241 ttgttgtccc gctggcagaa atgtttggct attcaacaga catacgttca aaaacccaag
      301 gaagaggaac tttctcaatg gaattcaaat gctatgaaca agttccaagt aacattgaaa
      361 cagaaatcat tgccaaagca ggcggtaata aagataaaga ctaatcgctt ggttaataaa
      421 aatcaaaagg gcggatttct taagaaatcc gcccttttgt ctataattat ttgttataat
      481 ttaaaaagat taatctagag gcacaattaa attttgacct atctgtaatt tgttagggtt
      541 aaccattttg tttgcaactc ttattttttc aacttttgaa gggtcaaatt taccgtagaa
      601 ttgaacaata atactttcaa gagtatcacc gtctttaacc gtataagtct cattttttgt
      661 gcttgttgcg gtttcattgt ttttgtctga tggtataaaa gttaatcttg ctttttcagc
      721 tttcattctt tttggaggtt tgatatcctg agtgtcatct cttcttacag aaaagctgag
      781 caaggttgtc atgataagca ttgcaaccgc accgacgata aaccctgtgg caagataaat
      841 gcccggtgat ttgtcttctt tttggcttac acggaagttt tgccaaagaa cgtcaagttc
      901 tttttctttg cttgcgcgga cataaactcc gggtgaagca tcataaggct tttgcctgtt
      961 tttggctaat tcctctaaca ttgccatccg ttctttggaa agatttgatc ttcttaatgt
     1021 ggaacttttc atagaccttc tccgtatttt aattcttaac aacatgatat tagcaaggca
     1081 tgccgtttgt caagaaatat atattttgta ttacatttct aaacattttt accatgctct
     1141 tatataaaac cggaatataa aaatttttgc tagtaaactt ctacatctaa tggtgtatat
     1201 tttgttcatt ttattaatgt tttcgccttt tttgacgact taaattaagt tatacacaat
     1261 atgttgggag ttttacatgc ttgaacaggc aattatgggt tcaaatttat caataaatct
     1321 gcaaaaagcg agagaatcat tagaaatagc aagagaacat catgaaaaat atttagtaag
     1381 gctgaataat caggatttgc aatttgcgat tgagcattat attaccgcaa ttaagttgga
     1441 tccgagtatc cctgaaactt attataggct tgccagttta atgtgggaaa atggacaaat
     1501 ttgtcttaat accgctattg agcagtgtaa aactgctgtt tctattgctc ctaacaacat
     1561 aaatgcacat atttataccg gttatttttt aaagttagcg catgaattta attctgcaga
     1621 aaacgagttt aaaaatgcta taaaacttgg aggggttaat tcggcacgtc caagattaat
     1681 tttatcgctt gctttgcttg aaaaaatgaa tactcaaaaa acttctgttc gtgaatttgc
     1741 aaaatgcctg tactatttga ctacaggaac gcttatgatg gcatgggatt tggcatcatt
     1801 gaaaatgctt tataaaaatt ttgctgaaga ttttagtatt ttttcttata aaaatgcagg
     1861 tgaattttgt gaaaaattta atcgcttaaa tgctgcggca aaggtttatg aaaaaggggc
     1921 agaaattacc ggatgcgaag aactttttta tcataaaacc gctgatattt gtgttaaaaa
     1981 acgggaaatg gaatctgcgg ttgaaaatta ccaaaaagtc ttggatgcaa atccgaaaaa
     2041 tcgagacgtc ttaattaagt tagcaactat tatacaaaca ttctttcctg atgactctga
     2101 tatagcaatc gattgttata ataaacttct tgaaatcgaa gaggataatg ctaaaattta
     2161 ctatgaatta gggcatcttt atctaaataa aaacgacaaa atacatgcct taagtgcttt
     2221 taagcttgca cttgataaag acagcgaaaa tcctttttat aacaatgcgc ttgcctatgc
     2281 ttttgttcag gcagaacttt atgatgaagc tattgaacat taccaaaaag ctattaagtt
     2341 aaacccagat aaacaatgga cagctattgt ttgtcaggct ttaggagcaa tttattatca
     2401 gattaaagaa aattttgagg ctgcagtcgc atcttttcaa gccggaattg ttcttgatcc
     2461 cgacaatagt gaaatttacc tttctctcgg tgatgtttat atggcagaag acgatttgga
     2521 taaggcaata agagcttatt gcgattcaat aagtataaat cctgaagatt atcgcgccta
     2581 ctctaaagca ggacttgcac tttgggaaaa agattatctt gaagaggcga ttgttgctta
     2641 ccatagagca atcgatttaa atcctgatta cgatattgca cacaataatt tgggcgtaat
     2701 ttatcttgac ggtatcggaa caccagatga agcgttaact tattttcaaa atgcaataga
     2761 aataaatcct aattatacat tggcttattt taatgctgga agagcacttc aggcacttgc
     2821 caagaataat gatgcggcag attattatca aatggcaatg gatttgaata aattaactca
     2881 agacatcgat gaagaagaca ttaaagagcg tttgtacggg ttgtttaata tctaatggaa
     2941 attttttctt ttatcatgca atttatagca acttgtcgag catatttgat gtacaattaa
     3001 tttatgaaaa agaaaataag cattataggt tcgacaggtt cgataggtac tcaaaccctt
     3061 gaagttattg aaaaacttca agataagttt gaaatcattg cgcttgcggg cggagataac
     3121 gttaaacttt tacgtgaaca gattttaaaa tttaagccga aaaaggtttg tgttaagtca
     3181 ctttctgcaa gaaatgaact taaggcagaa ttttctaagc ttgaaatttt ttgcgatgac
     3241 gagggtttag atgaaatttg ctctgacagg agtaatgatt taattgttgt tgcggtatcg
     3301 ggtaaaatag gcttaaaacc tacattaaaa gctattgaaa acaaaattga tatcgctctt
     3361 gcaaacaaag aaactcttgt tatggcagga gatattgtta tgaaaaaggc tattgaaaac
     3421 aatgtcaaaa ttcttccgat agacagcgaa cattctgcac ttcatcaatg tataaaagat
     3481 atttcaatgg tggaaaaatt aataataaca gcatcaggcg gaccttttct ttataaatcc
     3541 cttgaggaaa ttaaaaatgc aacagttgag cagacattgg ctcatccgaa atggaagatg
     3601 ggcaaaaaga ttacagttga cagtgcgaca cttatgaata aaggacttga agtcattgag
     3661 gctcatcatt tgttcggatt tgattacgac aaaattgagg ttattattca tcctcaaagt
     3721 cttattcatt cagctgtaga atatgttgac ggaagcgttg tggctcaaat cggattaccg
     3781 agtatgcata ttccaattca gtacgcaatt acttatcccg agcgttttga aggtataaaa
     3841 tcaaaaagct tcagttttct tgatgcaaaa cgttttgact ttgaaaaacc tgatttggaa
     3901 aaattccctt gcttaaaaat ggcttatgag gctggtaaaa tgggcggaag tgctacggta
     3961 tgtcttaatg ctgcaaatga agaggctgtt ttttcttttc ttgaaggtaa aatcaaactc
     4021 tacagaattt ctgaaatagt agaaaaaata ctttcggaac ataaattagt caaaattcct
     4081 tctattgaag aaatttttga aattgacaaa gaagtcagga taaaaaccag agaatttaga
     4141 agttagcttc aaacgggtcg ttatctttgt aatagtttga ttttttcttt ataaaaaatc
     4201 tttcattttt cttttttgtt tcgtttgaaa cttttctgta agcattgtct atagaaattt
     4261 tcgcaatagc ttttctgctt cttctgtcgt agaaagttaa ccagaatttt cttttgacat
     4321 tgtcaacaga aaaagaaacc cttcctgcga ctctgtcacc tggtttgatt tctgtaaaca
     4381 ttaattttgt gtcgccgaaa attgaagggt gaaaaactga gttatagtcg tttaccaatg
     4441 ccaaatcaaa gcttgaaaat gtcatttttg ttctgtttgt tattactaat gataatgtaa
     4501 ttgtatttgc ttccccgaaa gggtcatttt catatgttat gttgtttatt ttcacatcta
     4561 aaccttcgca aaacatttca tgctgcatca gtgccgtgtc tttataaaca ggaagaggaa
     4621 tggaagtatt tattttaacc cttatttcat cccccggtga tattaaaacg tcttttcctt
     4681 tgcggtaaag cgatgcacca agtcctatgg cacctcctac ggcggctcct cctgctactg
     4741 tatagccttg cgaagcaatg gctgcttcaa gtccgaagaa atttagggca acaattccgc
     4801 ccacaactcc tcctactgct gtatagccga tgtctgtggc gacaattttc cctactgctt
     4861 ttgcactgct tgcttttgtt gtcatttttc cttcaatagg aatttctctt ccgtcaggtg
     4921 ttataaggta gtcaaaatca agcgttaatg aaccatctcg cccaagcctt tttgcccctt
     4981 ttgattcact tattttgccg tgtgctactg ttcccattgg gattattaca cctgtaggcc
     5041 ctgaaacttc attagttact tctgcaaaaa actcgtcgcc ttcttgggaa aaactgcagt
     5101 ctaaaacctg tgatactgtc attcttatta cgtcttttga atcaagcgtg tcaattttgc
     5161 ccgtaaaaag ttcgctttgc tcttttattt gttcggattt ttcagccctt cctttaaata
     5221 attcaggctc tgcaattgcc gctattaaac agtttgcaga tagaaatacc cctaaaaaca
     5281 atattgttaa aatcttttta tacatcaact tctcccaaag ctaatattat tataactttg
     5341 tttttgtttt tttccaaata attaataatg ttaacattct gtaatattta gtttataaaa
     5401 ggcaatattt ttatgaagtt atactttata tatacataag ggatattaag agacttaggg
     5461 attacaattt catagggagg ataaaattat ggctattggg gcacgcgatt atattgacca
     5521 attattggtt aaaaaatatg cacaggaaaa agttgataac cacgataata tagcatcaat
     5581 ggttagtcct gaaaagatga tggaagctat gaacgattat gcagcaatgc atagaaatat
     5641 gtttgcacta agccagaaat taacttcagc atgttatgca ataaatgcaa ggagtgctaa
     5701 atacaacaaa gccgtaaatt tttcataagg gttactctcg gcaaaaacgg gtgttcttag
     5761 aacacccgtt tttgccatgt ttttgtgtta atataattat caaatgaaaa agattatttt
     5821 agcatctgct tcgcccagaa gaaaagaaat acttcaaaat gcagggtttg actttgaaat
     5881 taaaccttct gattttgatg aaaaattaga tgatttagat ttttcttatg aaaaaataga
     5941 aaaacttgct tacgaaaaag ctttttccgt tgcaaaaaag ttatctgaca aaaaatatgt
     6001 tgttggggca gatacggtcg tagtttttgc aaataatatt ttaggcaagc ctaagaatac
     6061 acaagaagca attaatatgc ttaaaatgtt atcagggaat gtccattgtg ttgttacatc
     6121 aatttgtgta attgaatctg ctactatgga atttaaaatt gcatctgtaa caacttatgt
     6181 agaatttaac aaattatctg atattgaaat aagtaattat gtcaataagt ttatgccttt
     6241 ggataaagca ggtgcatacg ggatacagga attacccaaa aattttgtaa aaaaaataga
     6301 cggaagcttt gaaaatgttg tcggattatg tcctaaatca gtccaagatt tattaaaaga
     6361 attttaatgt aaagtattat tttttattgc gttttgtttt cttattaaat attctttgta
     6421 aacttttgtt aacaatttat atttgaatat taaagtatta tccttataat tccgacatgg
     6481 ggggtatagg gaaaaagaat tgttacgaaa aaaggaaaaa ggtcgaattt aaggagaaac
     6541 tacaatgggt atgtcagcgt cacaggcaag attattaact ataacatcaa gaatgtctga
     6601 tttagaattg caagctcaga atattactaa caataaaatt aggctttcaa tgaaatctca
     6661 agaaattgct caaaactata gtgatgcatt gaataataaa aagcttgttt tcaataagac
     6721 tgatatttca gttacagcta ataatttgac atcatacaat aaagattctc ttgatgtgca
     6781 aagaatatta aaagacagta gcggcagggt tgttgtatcc caaagcatta tagatgcttt
     6841 taatgttgat cctaaccatg tatcttcttt ctgtcataat ttgcaaccgc cggtaaaaat
     6901 ttctgcagat gatgaagctg tacccggaga atttgatcta gcaaaattta aacactattt
     6961 taatctttgg tcacagttac gtgattgcgg atataatatt caaagtgatg ctaatttgaa
     7021 taattcggat tggttacaag aacaattgtc taacggtaac ttgatgattg ctcaaatgaa
     7081 atcaaatgct tggtatgggg caaatggagc accatttact cactctgttt ttgatacaat
     7141 ttcttacagt tcagatacca gcttagttga agaacttgat gaatcagatg atgctgctgc
     7201 agaagcagag tataaggtta aaactgctga acttcaggct aaagataaaa taatggacaa
     7261 tgagttaaac caaatacaaa ctgaacacaa agcacttgaa accgaatatg attcagttaa
     7321 aaaagttatt gagaaaaata ttgaaaaaac ttttaaaatt ttcggttaat tcttaactaa
     7381 gttttcttta taaatttcag gttcacaatt tttatttaaa acaaaagtat tatccttgtt
     7441 taattcaact tcaattaagc agggattttt actttcaagc ttttgtttta aagcttcgat
     7501 tttgtaattt ttgtttattt ttaagtaatc cattgaataa atttttgcaa tttgttttaa
     7561 atcgggtacg ccatattcgt ttcctgttcc gttgtatctt gagccaaaga atttgtgttg
     7621 taattctttt atcattccaa gggttgaatt gttgaaaaca aagcatttta tatttatttt
     7681 tttgtccgaa atcatttgta attcctgcaa attcatttga aatccgccgt caccggaaaa
     7741 tgcaaaaaca tttttatttg gttgcgcaag ttttgctccg attgcagcag gaagtgaaaa
     7801 tcccatgcag gcaagccctc ctgatgttaa tatgcgttga ttttttttga ctttaagtcc
     7861 ttgagctacc cacatttggt tttgacctac gtccgttgtt attattgcat cttcgggcaa
     7921 atattttgaa atttcactta taaatttaac cggatcaagc ccatttttat ttatttgtgt
     7981 attattttca tatttctttt gccatttttg aattattttt tcccattggg ataaatcttt
     8041 catttttaat gtatttagct gtttatttaa ttcgattaga aacgcttttg catcaaggtt
     8101 tatgcttaaa tcttctttgg tcgccctatt gagttcgtgt ttatctatgt caatgtgaat
     8161 aatttttccg tttatattaa aatgatctcg attaaaacca aaatgtcttt tggaaaatct
     8221 tgagcctaaa aatatcatta aatcagcatt ataaacagta agattggcag gtgttgtacc
     8281 ataaagaccc gaaaaaccga catttgtttt tgaaaaaata tccaagccca taagtgttga
     8341 aacacttggc atatttgtta tttcaataaa tttttcaaat tcattgagag cctcggcact
     8401 tcttaagcct cctccgacta ttacaacagg tctttttgct tttgaaatca tttctgcggc
     8461 ttggtttatt ttttgtaaaa ttttatcgtc tgtttgggat ttttcttttg ttttaaaatt
     8521 gtccaaatat ttttcttcaa tttcttcgtt ttgtatgtca aaaggcaagt caattaatac
     8581 agtgcctttt cgtccttcgt ttgctatata taaggctttt tcaagctcat atttgatttt
     8641 taaggcatct gtaattgttt ttgcatattt tgtgactggt ttgaccatat taatggtgtc
     8701 aacttcctga aaactgtgct ggcgaatgtt taaaaaagac ggatttatat cgaaagtatt
     8761 gacctgtccg gtgaaaaaca aacaagggac agagtctaaa tgagcatttg ctattgcgct
     8821 tatcaaattt gtcactccgg ggccgtttgt aacgagcgcg attcctgttt tgcctgtaac
     8881 tctggaataa gcatcggcgc aaaagcctgc agcctgttcg tgatacgctt gaatatattt
     8941 aacttttcct gtttttacaa tggcgtcgat aagcggattt atcgcagctc cctgatagcc
     9001 aaaaacttgc tctattccaa gattttcaat atattttgct atgtaatcta cgactttcaa
     9061 aaaaattttt ccttaatttg tacttgacaa ataccattga taagcagctt ttattccctc
     9121 tttgaggtta attttaggac tccagccaag cgactttatt tttgttgcat ccattaattt
     9181 tcgaggagtt ccgttcggtt tagatttatc ataaacaatt ttgccttcaa aacctacgat
     9241 ttcttttacc gtattaatta aatctttaag ctgaatgtct gtaccgtcgg ttatattaac
     9301 caattcgcct atatcgttat aatttttatt ttgcatcagg taaaagcagg catcaccaag
     9361 atcatccgag tgcataagct cgcggtaagg cgaaccgtca ccccaaacaa ccactttatc
     9421 tgagtaagct ccgatacctt ccagtgcttt tgtgattgaa gttttgtcat taaggtttag
     9481 tttgtcgtct aagccccaac caagcttata tttttttaaa tcggctttta tcgcttcgaa
     9541 atcaccgttt ttgagcagtt tggcaagatg aaaccgtctt aaaagcatcg gaagaaggtg
     9601 tgccgtttcc atgttgaaat tgtcacccgc gccgtattgg tttgtgggca taacggaaat
     9661 aaaatttgtt ccgtactgtt cattataata cctgcaaagc tttatagcgg caatttttgc
     9721 aatagcgtaa gcttcgtttg tgctttctaa ttctgaggtc aaaaggcaat cctctttcat
     9781 cggctgcggt gccagttttg gataaataca gcttgagccc agatttaaaa gtttttttac
     9841 accgaatttg tagcttgcgt gaataacgtt tgaggcaatc attatgtttt gataaataaa
     9901 ttcagccgga taagttgaat ttgccatgat tccgccgact tttgcagctg caagaaaaac
     9961 ataatcaggc ttttcttttt caaaaaattc ttctaccgcc ttttggttgc acaggtcaag
    10021 ttcgtgaagt tcttttgtga ttatattatt gaacccttca gatttaagct ttctcataat
    10081 agcagagcct acaagtccgc tgtgacctgc cagatatatt ttttcgtttt tttgcacttt
    10141 atacctcttg aggatttaat acctcataac cgccctgttt taagaatact tcttttttag
    10201 cttctttcag gtcttcggcg accatttctt caataagtgt ttttaaatca tacttgggtt
    10261 tccagccgag ccttctttgg gctttggtag catcaccgag aagcaaatca acttctgtcg
    10321 gtctgaaata acgcggatcg acttcaatga cggttttgcc tgttgcttta tctatacctt
    10381 tttcgccgac acccctgcct ttccattcaa ggtcaattcc gacttctctg aatgccatat
    10441 tgcagaaatc tcttattgtg gttgtaacgc ccgttgcaag aacgtagtct tcgggtttat
    10501 cctgttgaag gacaagccac atgccttcga cataatcttt tgcgtgtccc cagtcacgtt
    10561 tcgaatcgag gttccccagg aaaagttttt cttctattcc gagtttaatt cttactgctg
    10621 ctcttgtgat ttttcttgta acaaaagttt caccgcggcg agggctttcg tggttaaata
    10681 aaattccgtt gcaggcaaaa atgttgtagc cttcgcggta atttacgcta atccagtaac
    10741 cgtaaagttt ggcgcaagca taaggtgagc gcgggtaaaa tggtgttgtt tctttttgag
    10801 gagtttcttg tacaagcccg tataattccg aagttgatgc ctgataaaat tttactttct
    10861 tttctaattt taaaattctt atcgcttcta gtattcttaa tgtgcctaat ccgtcagcat
    10921 ttgccgtgta ttcggggcaa tcaaaagata cttttacatg agattgcgct gctaagttat
    10981 aaatttcatc gggctgaatt tcttggatta atctgattaa attcgtgctg tcggtcaaat
    11041 cgccgtagtg caatttgaat ttaagatttt tgttatggac atcttgataa aggtggtcaa
    11101 ttcgtgccgt gttaaagctg gatgaacgtc ttttcatgcc gtgaacctca tatccttttt
    11161 taagcagaaa ctctgcaagg tatgagccgt cttgtcctgt aatccccgtt attaaagcta
    11221 ctttagccat ttaaccccct taatttttgc gtgttacata aagttagaat acatttaccc
    11281 caaattttag tataaactta ggactctttc aagtgtttaa aatgatttat taagtacagg
    11341 acgaataatg tccccaatac cagaacaatt gctaaatcaa atttgtgcca gaaggcttta
    11401 agggcttctg tgtgttcccc gagctttatt ccgacatata caagcggata actccaaatc
    11461 aaagaaccca aaaacgtaaa tagagtgaac attttaatgt ctgcctttaa aatccctgca
    11521 ggaagcgaga tgaaagttct tacaatcgga agcatcctgc agatgaaaaa tgcgaggttg
    11581 ccgtattttt gttcccattt gtccgccatt tcaaggtcgc ggtggcttat cagaaaccat
    11641 tttccgtatt tttcgacaaa aggtcttccg ccgaagtaac ccaaataata tgaaggaatt
    11701 gagcctaaaa cgcaccccaa agcgcctgca aaggctgctg tatgaattgt catgtttcct
    11761 ttacttacaa gatatccggc aaacggcaaa atggcttcgc ttggaagcgg aacattgcac
    11821 gattcaatcg ccataagcat tgaaacgccc aaaggtccca ttgtgcttat tgtagtgtgt
    11881 ataaaatgca ccaccggtgc caatattgaa tctagtgcgc tcataaattc tccttccaac
    11941 cgctttattt attttacttt aaaaattttt cttgtaaatc atttttgaaa tgatattctg
    12001 aatttgttgt tacgccgttt ttaaaggagg gttattatgt tgaattttga attttgttgt
    12061 cctacgaaag tggttttcgg caaaggctca atcgctaagt tgtctgaact tatcgataac
    12121 gataaaaaga ttttaatgat ttacggcggc ggctctatta tgaaaaacgg agtttacaat
    12181 caggttaaag ttgcgctgaa tgattttaat atgattgaat ttggcggaat cgaacctaac
    12241 ccgacttacg aaacctgcat ggaagcggtt aaaatagtta aaaaagaaaa aataaatttt
    12301 ctccttgcag tcggcggggg ctctgttttg gatgccacaa aatttatcgc ggcagctgct
    12361 aagtataaag gcgaagagcc ttatgacata atctcaaaat gtgctccttt tgatgagttt
    12421 ataccttttg gaagtgtaat aacgcttcct gcaacaggct ctgaaatgaa tgccaacacc
    12481 gtaatatcaa gaaaaagtac tcaggaaaaa cttgcgcttc aatcaggaaa gctctatccg
    12541 actttttcta taatagaccc cgaagtcact tttacactac cgacaaaaca aaccgtaaac
    12601 gggattgttg atacttttgt tcatgttatg gagcagtatg ctacttatga tgttaattcc
    12661 cccctgcaag actcatgggc tttgggtttg attaaaactt taataaatga atcgcctaaa
    12721 gtccttacta atcctaaaga ttacgaggca agggcaaata ttttttggtg tgcgacctgt
    12781 ggacttaatt tttggttatc ctgcggactt gttcaggatt gggcgacaca cagaatagga
    12841 cacgaactga ctgctcttta cggacttgat catgggcaaa gtcttgcgat tattttgccg
    12901 cgcctttggc aatacaagaa aaaagacaaa gcaaaaaaac ttgctaaaat ggcaagagac
    12961 gtctatggct gtgttgaaaa agatgaactt aaagcggctg attacacaat taagaaaacc
    13021 gaagaatttt ttaactctat cggtatgaaa actaagcttt cagattatgg cattaatccg
    13081 caggaagctg caaaaattgt caaagaaaga cttgagcaaa gaaaagttgt atttggtgaa
    13141 aataatgaca ttactccgca agttgcttat gaagtcttgc ttaagtgttg atttaaaaaa
    13201 agggggcgaa gctaaagctt cgcccccttt gaaaatttaa atttcaaaga ttatttttta
    13261 cctttacagc agcagcattt tctcatgcac caaattgcca gcgcaggtac tagggtaaat
    13321 tggattatca aaattttcca aagtcccatt aaaaatacca aaagcatact gtaatcgtca
    13381 acatcaagtt ggaacaattt ttccatcatt tgagcctgat agtcaaagaa aaatatgcac
    13441 attaaagatg aaaaaagcag taaaacaaaa ctgataataa aagctttgaa gaaataatgt
    13501 tgcatgtgct ccaatttttc ttcgtgtgaa atgtcgcatc ccataaaaac caaacctttc
    13561 tttttttaac tttcgagatt gcagaaaaat taaaattttt ctcgcaatct cccttgtccg
    13621 gctttcatcc ttgccgccgg ctatctcgca gccggcaagg atttagcact tctaatgtcg
    13681 gacttgaaaa attgcgaaaa attgtcattt ttcgaatttt cccgcatcct cttttgcatt
    13741 ataggttttt gtttagagct tgtcaatagg acaaggtatt attttcttat tgcaacaaac
    13801 ttgcattgag ttggattgaa ggttaaaata aagtcatgta taaatttaag aagttcgtta
    13861 aaattttatt tagcaaagtt tttttattga ttgtttttgc tgcctttctt ttgagcatct
    13921 ttttttattg gggctggtat gaaaggcagt atgacaaagg cttaggcatg tattatgtct
    13981 ataaaggaga caaggcttac caacaaaggg atttacaaaa ggctatcggt tattataata
    14041 cggccttaga agtttttccg tctcattacg gtgcaaggct taatttaggt aatatttttg
    14101 ttgtttatga agattattat gctgctgcag attcttatga aaaagcaatt gaatataata
    14161 aaaatttcag tgaagcaaga atgaatttag gtattgtgtt gtctgaaaaa atcggtgatt
    14221 ttgattctgc aatcagccag tatcaagcaa ttataaattc aaagccaagg gtattttcaa
    14281 ttccgtttat ttttgacaac aaaaaaagtg aaaggattaa tcgcggactt gcttactata
    14341 atatgggaat tgcgtacagg caaaaagcct tatatcttga agatgaccaa gataaagaag
    14401 aaaaagcggc tatgttttta agaaaagcta ttggggctta taaaaatgct gtcagaattt
    14461 taaaaagtga ctatgattcc agatataatt tagctttggc atatcatttg gtcggcaatt
    14521 atcaaaaagc agggcttaca tattgtgaag cgattgatat tgagccgatg aattacgaag
    14581 cccattataa tcttgcaata cttttgagac atctgagaat gtatcaagaa gcaaaagaag
    14641 agcttgaaaa agcttctata cttatttcat ctacacattc ggattcttac acttcacgat
    14701 atatctttga tgttttaaat gaagttactc acaatgcttt gtacaggaca gaatatgagc
    14761 atttaagaga aaaaatcgac gatgaacctt cttatgcaca aaaagttgcc tacgttcatg
    14821 gtaaaattgt tgcctcggat gaacttgaca aagctatgat taaaaatttc agaaattgca
    14881 gaacaaaaaa acttttcaaa gattacgagt aatatatgaa aaataataat tttgaattga
    14941 acttactggc tgagatttca aaggttttga cagaaaattc aaatccttcg gatatagttt
    15001 tacttttgag taaaatattt tctgattttg cggatttatc caagttgagc atttttattt
    15061 acgatgaaaa cacaaaactg ttgaaggatt attcaaaaag ctgggttgtt attgatgaat
    15121 ttcataaaaa tttttatacg gataaacttt atattgcgct tgcaaatttc agccaatggg
    15181 atttttatat aaatgataaa aagtttaaga cactagattt taaattctct ggcgagatta
    15241 aattaacaaa agatattaac aaaattttaa tccctttgaa acgcaagaat aagccttatg
    15301 gaattgttga attgagtttt agcggtgata tttcaaaatt attaacttat gaatttttta
    15361 ttgcactttc ggttgtttca tatcagcttt cgttaaaaat tcaaaacaca attctagccg
    15421 agcaaatgca gacaaatatt gattttcacg atgcaatgaa aaatattgca aaaattattg
    15481 aaactcaata cgaacttaat tatattattc ctcttattgg tgaaatgatt gacagattta
    15541 tatcggaaca tcttgtttat atcttcttaa aaaacgaaaa aaacgggaaa attgagcttt
    15601 tttggccaag agcctgccgc gataaaaaga ttttgtcggc tgtttcaaaa gttaatacaa
    15661 aatcaaaaat cacattgctt gatgatgaga agataggaat ttttcctctt atcggcgaaa
    15721 aaactttgct ggggtgcatt gtggcacaca gcaacgttga taagcttaca aaaaatgaaa
    15781 tcgaatattt agaacagttg tcaaagcagt cttcaataac aattcacaga gcggaagttt
    15841 atgccgaagt tctgcaacac gccacacttg atgctttgac gggtttaaat aatagaagac
    15901 agtttgaaat gaggcttaag caggaatatt ctaccgcaaa acgtcagcac aaaccgcttt
    15961 gtgccatgat gattgatgtg gattttttca aaaaagtaaa tgacacatac ggtcacaatg
    16021 caggtgattg tgttttgaaa aatgtcgcaa atcttatcaa ggcggggctt agagactatg
    16081 atatcgcctc acgctacggt ggggaagaat ttgcggtttt gcttccatat acaaagattg
    16141 aagaagcatt tgctgttgca caaaggctta ggcaatccat tgaaaaggca aaaattgata
    16201 tttcaggcga aggtgatgtt gatgttaaac caatcagtgt taccataagc atcgggcttt
    16261 atcagtttaa atccgaagac agccttgaaa tactttatca aaatgcagac aaggcactgt
    16321 atgatgctaa aacacacggc agaaataagg tggttatttt taaatagtca atgaaacacg
    16381 tcgttgcgag gggggaaatg atttcggatt tatgattcgc ccctcacgtc tttgcaagac
    16441 aaaggttatc ggcttttaca attattttcc accgaagcaa tctaatcaac ttgctcatga
    16501 taaaaagatt gccgcgctcg ctttgctcgc tcgcaatgac gtcacacttt tcgactttat
    16561 tttattcaat tgattataat tactctttta attctttttc aaagccgaaa agcgaactta
    16621 cggattcatc gtcatgaatt cttgaaattg cttcacccaa aagtggtgca actgaaagct
    16681 gtttaatttt tgcgggcata ttagacatat caacaggaat tgtatttgta attataactt
    16741 cggttatagg tgagtttgta agtctttcta cggcaggacc ggagaaaacg gcgtgcgctg
    16801 cgcagacata aacatctttc gcaccttctt ttttaagcag ttttgccgct tcgcagatag
    16861 tgcctgcggt gtcgattata tcgtcaaaca taatgcaaat tttgtcttta acatcaccga
    16921 taacgtgtgc tgcgactgct tcattgtgtt tatctcgtct tttgtcgatt attgcaagcg
    16981 gacaatcaag ttcttttgca aaatatcttg ttctcgggac tcctcctgtg tcagggctta
    17041 ccgcaacaag ttcatcggat gtaagattca gttttttaat gtaatctacc aaaataggtg
    17101 ttgcaaaaat gtgatcaacc agaatgttga aaaagccctg aatctgacct gtgtgcaaat
    17161 ccattgcaag aactctgtca gcacctgcgg ttgtgagcaa atccgcgaca agttttgccg
    17221 tgatggcttc tctgcctgat gtttttctgt cctgtctggc gtatccgtaa tatggaatta
    17281 ccgcagttat tgtttttgcg cttgctcttt tgaaagcgtc aattattatc aaaagctcca
    17341 taaggttttc gttgacagga ttacaaagag gctgaactat aaaaacgtca tctcctctta
    17401 cgctttcttt aacctgaaca taaatttcac cgtctgcaaa gttttttata accataggtc
    17461 cgacggttgt tcccaaataa tttgcaatct catgtgtcaa agtataattt gaacgtccgc
    17521 taaaaagctt tatatcgtgg gttggacgaa tagctttttc cagttgggga aaattcattt
    17581 ctaaaaccat ttcaaagtcc ttcctaaatt gtcatccgca caattattat aacaactaag
    17641 gatttaaact tcaaggaata tagattaaat atgaagtttt actttacata aaaagaagca
    17701 tcaacattgg cataaatttt tattttacca ccttcaatag gcgttgaagc cgcagtgtct
    17761 tgagttgatt ttgccattaa ggtgtagtat ggcgcaggat tattttgggt ggagcaggtc
    17821 gtgttaattg attttacgcc tagtactttt gaattcattg ttgtggcaac ggatgaagct
    17881 tgtgtataag ctttttttgt aagttccgca agcaagtcgt tgcatgcgct gtcgtaatct
    17941 gccgcgaaga attcaagatt atttacattt gttgcgccta attcgattga cttgtcgata
    18001 atttttgaaa ccaaatcaac gtttttggtc tttacgctga ttgtgtttga gactatatat
    18061 ttgtcaaaaa cttttttgtt ttcttttgta taaacataaa ccggccttgc agagtaattt
    18121 ttggttttga tgtaatctcc gcttgccgtg tttatgatgc tttttaagcc tgagtaaact
    18181 ttttcacaga ttgctttgtt atcatcggag gctttttgaa gagtttgggc ggaggtttct
    18241 acggcaatag aaatttctgc gatattgggt ggaatttctt ttgtctgcga aaaacttgtc
    18301 gaaatatagc cttcttcttt ttggggttca gcggctattg caacaacaga aataagcaat
    18361 acagttaaaa atgcaaaaaa gatttttttc atggatgccc ccttataaaa atttattctt
    18421 tgttttcaga atgtaattca aggtcttctt ctgtttgatt tttattttca atgtttttgt
    18481 ccctgtcctg aagaagcccg ttttttatgg tttgtgctct tatttttgaa ataacttcgc
    18541 tcaattcttc atctgaaagt tcctgccttg cattaaacga tacaataaat ttttttttat
    18601 taaaaatata aatgctcaaa actattatgc cccaaagtaa aacgccctga gtaaaattaa
    18661 tcggaggaaa tgaacctgta gttgaggaaa gataattcca cccgttcatt gtcaaaaatg
    18721 ccggaaatgc aataaagccc gcaatcaggc aactaactat aaaaagtccc aataaaatcc
    18781 ctctgaatcc gtttatttga ataattttta aatttttttt cattatatcc tcaacctaaa
    18841 ctttgtttat catgtccaaa aattcatgct ctgacaatat tataacacct aaattttgtg
    18901 cttttgttaa ttttgagcct gcgctttcgc ctgcaactac aaaagaggtg tttttactga
    18961 ccgatgatga ggtttttccg cctaaagatt taattttttc ttcggcttca tcccttgtca
    19021 tagtctgaag tgtgccggtt aaaacaaaag tttttcccgc caaaatgttt gatattttgt
    19081 tgcttgcttc ggatttcggt attattccaa gttcttgaag cctgtttaag aattcaagac
    19141 ttttttcatt tcggaaaaag tcaaaaacat ttttggcaat tttttcgccg attccttcaa
    19201 tttgtgccag ttcttctaag tttgcttttg aaacattttc gagtttttgg aagtgttgag
    19261 cgataatgtg tgctgtttct ttgccgacat gtcttatcga aagcgcggtt aaaaatcttg
    19321 ttaatgtcgg gtttttgctt gcttgaatcg aattataaat attaaaggcg gatttgtcct
    19381 gaaccaaatc caactgcatg aaatcctgct gcgaaagtgc gtacaaatcc gccgcagatt
    19441 taaccatgtt tttgtcgaaa agctgctcga taattgatgg tcctacaaaa tcaatgtcca
    19501 tagcctcttt agaaacccag tattcaattt ttgcctttat ttgagcgggg cagaaaagtg
    19561 aattcggaca ataaagattg acttcgcctt ctcttgtttc aagaggagta tgacaaacag
    19621 ggcagtcgtt ttgcggggta tattgagaag aatttggtgc gtgttggctt actttaatta
    19681 cttttggaat aatttcagcg gcttttttga ttaaaaccgt atcgcctatt cttatttcaa
    19741 ggcgtttaat ctcatcaaaa ttatgcagac tggcacgccc aactgtgctt cctgcaagct
    19801 gaacaggctc taaaatcgca accggagtca ccgcaccggt ttttccgaca ttggtttcaa
    19861 tatctaaaag ttttgtgttg acttcttcgg gcggaaattt gaaggcggtt gcccattttg
    19921 gcgcgcgcga tgtgaatcct agttcttcct gcagtcttaa gtcgtttact tttattacaa
    19981 ctccgtctgt tgcataatcc agttcaaatc gctttgtttc ccagtttttg caatattcta
    20041 ttgcctcttg aatgttttta acggcttggg tattcgggct gatcttaaat cccagttttt
    20101 ttagcagcat aagattttca tagtgcgttt ttggttttaa atttgtatct gttaaaatcg
    20161 cactgtagca aagcattgac aaatctcttt ttgccgtaat tgaactgtct aattgtctta
    20221 cagagccgct tgcggcgttt ctggggtttg caaatatttt ttcgccgttc tcaagatttt
    20281 cttcgttaag tttttcaaaa gaggattttg gcatgtaaat ttcgccgcgt acttcgagtg
    20341 agaagtgaga ggtgagaagt gagaggtgat gggatggttc aggcgtttcc ccctctcccc
    20401 ttgcgggaga gggctggggt gaggggttaa gcttcaacgg aattgcctta attgttttta
    20461 ggttgtttgt tatattttcg ccgacttcgc cgtcgcctct tgttgcgccg gttgtaaaaa
    20521 ttccgttttc gtaagtcaga gaaatagcaa gcccgtcaat tttaagctcg cagacaagct
    20581 ccacgtcttc tccgacctct tttgtaattc tttcgtacca cttgatgagt tcttcataat
    20641 tgtttgaatt atcaaggcta taaagcctgt atttgtgttt gtgcggttca aagcttgaac
    20701 ttatgccccc gagtctttgg gtggggctgt caggagtaat taaaagaggg ttttcgcttt
    20761 caagtttttg aagttctctg aacatttcat cgtattcaaa atcgctcaat gacgggtttt
    20821 cttcgacata gtaaagataa ttatgccgat ttatttgctc ttttaaaaaa tttattctgt
    20881 ctaaaatatt caatttgaaa cctttacccg aagctacccc tgcattaatt ctttaaaatc
    20941 aattttatgc tctcttgcat agtttataaa ttctttttcc tgttggctta cattaattct
    21001 gaattctaaa acaaccccgt taacgggttt tctgcctgca cctctacgtt tgccgcccca
    21061 tttgacgatt tctttgtcgg agttttcgat tattttttgc tttcttgcgt tcggtaaagc
    21121 ctttattaat tcgtcatttg ttgtcattgc tgaactcctt tttataattt tcgatatttc
    21181 tcatagcttg ttcgataaat cttttcggtg ctttctgcgt tttctttaaa actataagcc
    21241 ctatgatgat gattttgttc ttggcgtaca taaagtatgc cctgatacct tttggcttta
    21301 gttcgaaaag cccgtttcct aagctgttga tatttttgta attagttccg atatattcga
    21361 aaattcttac ggcttctaga attttgtctt ggctgttgtc gtctagttct ttaaattgtt
    21421 ctaaagcttc tttggtgtat tgtgagttaa attttttcat taatttgcct tactaatatt
    21481 atacttgatt aaacaccttt tttcaagtgc taaataaaat tatcttaata ttttaatata
    21541 attttattat aaaactcatc ttaatgcaaa cgaagcaggc tttataaggc tcttggaaat
    21601 atctgtggcg gtattcgtaa ctttaagcaa attaaatttt gaaaaatctg agtcgaatta
    21661 aaaataaata gttttaatat atcttcgtaa aaatcaaaaa tatgtgtatc catgctaaac
    21721 tatactttag ttatatggag aatttattat aaatggagat gtgttgatga ataaattaaa
    21781 gattcttgga gaaaagttaa ataaaatact ttgctatgat tatactaaaa ctttatctgt
    21841 tttatcctca ataatgttga ttttatcttt gtttacactt tctagtgtat attatgattt
    21901 tttaatatta attatttttg taagtagtgc ttttatatta ttcaggtact atcaaagtaa
    21961 ggaaaaaagt gtatttgtaa tatttttttt attggtttta ttgttattta atccatttat
    22021 accagtaata attgataggc aaatatggca aatattaaat gttttttcag cattaatatt
    22081 ttggatttat tttctaaaag attttagtga aaaaataaaa ataatagcca ttttaagttt
    22141 aattttattc ctaactttgt ttactccttt acctccgcaa gaaataaacc atcttaagat
    22201 attgggaaac aatggatttg tggttaaaat caaagaagat tcaatgtttg ctgaggaaaa
    22261 accaataatg catgaaacaa ttatccataa taatttaata actttcttta tacaaaaatg
    22321 tataaatttg attcctgtaa aaagtgatga agaaattaaa tttaatttta aagaatagct
    22381 tttattttta tgttacaaaa aacttttccg ctattttttt catagcgttt ttttggagta
    22441 ttttacatac acatcaataa ttcacgtata acttttgccg caaccgctgt tgaaactccg
    22501 cttgcatcgt agtgcggtgc aagttctacg acatctgcgc cgactatatt tagtcctttt
    22561 aattttttaa accagtcaat taattcgtta aaggtcaaac cgcctgcctc cggagtgcca
    22621 gtccccgaca ttattgaagg gtctaaaaca tccaaatcga cgcttacaaa aacgtttttg
    22681 ttaaccaacg gtgatatttg ctcgggaaag tttaatcttg tgtgatgttt gtccattaaa
    22741 tcaaattctt cttttgttcc cgagcggatt ccgatttgtt ttaaattttt aaacccgaca
    22801 atttttccga tatgatacat aaccgcagaa tgtgacatct cttcgcccaa atactctttt
    22861 cttaaatccg tatgagcatc aaaatgtaca accgtaatat ctttataaaa tttagaaata
    22921 gcctgaatcg caggaagcgt aactaagtgt tcgccgccga ttccgaagaa ttttttcccg
    22981 tctttgtata tctgttcgac gttttctttt ataagattta gcgacttttg agtattccct
    23041 agaggaaatt ctaaatcacc gacatcgtga aaattaactt cttccaagtc catgtcaaaa
    23101 taaggcgaat aggtttctat accccagctt gcaagccgaa tcatttccgg tgcgaacctt
    23161 gagcctgaac gataagtaac tgtcccgtca aaaggaagcc ccaacataac aacgtcgctt
    23221 gatttataat ccgggttttg ctccatccaa tttctgtcta aaaatggtcc taacattttt
    23281 tcattttact acaaaaaaga ggcggcggaa aatttatttt tccgccgcct ctaaaattac
    23341 tctgtctcgt taattcattc ctgattttga caaaaacatc tgttttacaa ggtcgcaata
    23401 aacatcaact tcttctttga ccttgtctat cccatttaca agttccctaa tctcaattat
    23461 tattgcttca ttttctaaat caaacattct atacaccatg ttccctacat tttatttttc
    23521 ggaatttata ggaaatactt tataaaattt ttgttctttg ttacaaagat taataaaaaa
    23581 gtaaatagta aatagtaaat agttatattt ccattttttt gttaatatta ttgaaaagaa
    23641 aagtttaagg aggagaaatg acaacacaga ttattgaagc acaaaacgga agaattaccc
    23701 ctgaaatgtt tgcagttgca gaaaaagagg gatataaaca ggaagaaatt cttgaaaaag
    23761 ttgcttcagg gcgtgtcgtt attttaaaaa ataatcgcag agaaaacgtt gttccaaccg
    23821 cagtaggtga aggaatgaca gtcaaaatca acgccaatat aggaacttct cttgaacgct
    23881 catcaataga agaagaactt gataaggtta aaataattga agcaacaggc gcggatgttt
    23941 taatggattt gtcaacaggc gataagattg atgaaacaag aaaggcaata attgcagcaa
    24001 ctaatcttcc aatagggaca gttcctatgt atcaagcggg aaaagaagca ctggacttac
    24061 aaaaagacat ttctaagctt tctaaagaaa aacttttttc tgatatagaa aaacattgta
    24121 aagacggggt tgactttatt actgttcatt gcggcgtgac aaaatttgtc gttaatcaat
    24181 tggaaaaaca aggaagaatt acaaatatag tttcaagagg aggctcaatg cttgcttgct
    24241 ggattaaagc aacgggaatg gaaaatcctt tgtacgaata ttatgacgaa ttgcttgatt
    24301 tggtgcgtga atatgattgt actttgtcat taggtgatgg actaagaccg ggttgtaccg
    24361 cagatgccgg tgacagggca caggtagcag aaaccattgt tttgggagaa cttgttcagc
    24421 gcgcaagaaa agccggagtt cagacaatgg tggaaggtcc cggacatgtt cctttgaata
    24481 aaattccttc aatgattgaa accataaaag ttttgactga ctatgcgccg ctttatgttc
    24541 tcggacctct tgtctgcgat gttgcaccgg gttatgacca cataacttca gccataggtg
    24601 ggacacttgc ggcctatcac ggagcagact ttttatgtta tgtgacaccg gcagagcacc
    24661 tcggtttacc agatgttaaa caggtcaggg agggaattat tgcaagcaaa attgcagctc
    24721 atgccgctga tttggctcgg gggaataaat ttgctattga aagaaatttg aaaatgtctg
    24781 ttgcaagaaa agatttggac tgggatgggc aaagaaaata ttcgattgat aaatgtgttt
    24841 ttgaaaaaat cgatcaaggc aagccatgta ctatgtgcgg acaatattgc agtatgaaaa
    24901 tatttaaaga atatttctaa tttgaatttt tgtaaacatt tatgctatta tgtttaaggt
    24961 catggctgtt tgtgtcgtta aggattatgg tgctattgtt taaggattta tattttgagt
    25021 attaattttg ttgacaagac tattgcatct gatttggaat atattcttct ttcttctgaa
    25081 agagggaaga agcataaggt gcttgctgtt gatgatgaag aaaataacct tgcccttatt
    25141 aaaagaacat tgaggggtga gtatgaagtt attattgctt ataatgggca agaagcactt
    25201 gaaataattg acaaaatggg cgatgaaatt tccttgatag tttctgatca aagaatgcca
    25261 atggtaagtg ggactgaatt atttaaaaag gtttcggaaa aatatcctga tataattaat
    25321 atattgttaa ctgggcattc aaatatagat attttgttgg attctgtaaa tgaatgtcag
    25381 ttgtttcaat atatacttaa accttttgac ccggaacacc tttgcatggt gattcaaagc
    25441 gggataagaa aatttgaaat gaccacatca agaagtcaaa ttttgtgcga tttatctgaa
    25501 ttattttata agactataaa gtcaattgct tttgcgcttg atgccaaaga taaatatacg
    25561 catggtcatt caatgagagt gactctttat tctcttgctc ttgcaaaaaa tcttgaattg
    25621 cccgatgatt tgttagaaga gattgaaaca gcaggtttgt tgcatgatat aggaaaaatt
    25681 gctattcctg agaaaattct tctaaaaccg gggaaattga ctgatgaaga atttgaaatt
    25741 ataaaatcgc atcccgaact aggcgaaaaa ttggtaaaaa gtgttgaaaa gctgggactt
    25801 atatcaaatt ggcttaagca tcaccatgaa agatatgacg gtaaaggata tcctgacgga
    25861 cttgtaggag aagacattcc tatttctgct agaattatag ctcttgccga tacttatgat
    25921 gctatgactt ctgatagagc ttacagaaaa gcgctatctc atgaagaagc tatagctgaa
    25981 gttgaaagat gctcgggcac acaatttgat cctaatctgg caaaaatatt tattgaaatt
    26041 caagaagaaa ttaaacattt aaaagaaaat tccgaacaag aatatgcaaa atacagttat
    26101 ttaaacaaat ttatgaactc taaatcataa atcgttaatc ataaaattac tttttttgtc
    26161 tcttggtttt gtaggggcga aaggtttatt cccaatgtct atttcttcaa tttttacaga
    26221 ctcgggggca agattatttt catcaataat tttgctttca acgcttttaa caggaatatt
    26281 agaagggcat aatttgtttt caactatttg tgcaatcatt ggagtaaaat aagatgaata
    26341 aaacttaagt ttttccaatt gttttgtcgc tggcgcaaaa ttaactccaa tcatgcggtt
    26401 ttcaacggcg gaaagatttt tttgaaaagt cccttgccat aaaacttttt cttcatcaac
    26461 atcaacaagc gcaatataag tcgatactct atatgcaggg tcaatcactg ccgcccccgg
    26521 gatatttaaa aaatcccaaa ccgtacgcct tagaaggtag ctttgggtat ctgttgtgct
    26581 tgtgattagc aaaactttgt ttgtatcaaa ggtatgtgct ataggtttta tgtcaacaaa
    26641 agctatgttg taattgtatt tatattcttt aagagctctt ctggttgtaa tatttagccg
    26701 tacatttttt ctgaaagcat ctcttatttc tgccatgctt acagtatcaa ccttgccgga
    26761 aagtcttagt gagttaattg tgtcagaggc aaaaattacg gaagtgtcag gataaatata
    26821 ataatttgtg ctttcaaact gaatattatc aggtaatata aggatttttt tatcctgcgc
    26881 aaaacaagtc tgtgtcgtca aaaacactaa taataaagct gaaaataacc agattaattt
    26941 tctcataacc ccattgtatc ctaatttata taattatcaa atatttttac aatacttaaa
    27001 tcctatgatt aattgatttt aatttggata ttttgagtaa aatttaaaat atggattatt
    27061 cgcaactttt acaaaaagct aaagatgctt caaataaggc atattgcccg tattcacact
    27121 ttcctgtcgg ggcttgtgtg cttaccaaaa gtggtaaaac atattcgggc tgtaatgttg
    27181 aaaatgcaag ttacggactg tcggtttgcg cagaaagaaa cgctattgca aatgctgcta
    27241 ttgcaggtga tttggatatt gttgctatag ctgtttatgc ccaaaaaatg gaaaattgtc
    27301 ttccttgcgg agcttgcagg caaattattt ttgaatttca aaaagataat gaaattgatg
    27361 ttataataca atttggggaa ggttataaaa tttataaaat taattatctt ttacccgagg
    27421 cattcagttt atagatgtat tttattgaat taataattaa aaaatttaaa aaaaaagaca
    27481 aacaattaaa agtttcaaat caaattgatt ttgattatga atcttgtgag catgttttta
    27541 tgccaattga tagcacaaat gaaatgtttt catgcacaaa atgcggcttt attagaaaca
    27601 aaaaagattt gaaaaataaa aacttttttg aaacttaatc tattcttcct tctccactac
    27661 cacagtctgc ttggcttgta cagctgttcc ctgtaggtcc ttgtgacgtt actacggccg
    27721 taccttcaat agttgtattt tctgcactat ttacaaggct ttgaaattga gacaggtagt
    27781 cactgcttcc tgcagctgaa gggttttgta tagcataatt aaaagcatct atcaatgcac
    27841 ttattgtttg agaattgata gttgaagggg acaaaggagg gtcaagattt aatgcagagt
    27901 ttgcatattc aaataaattc attggcattg gcgtagcggg agtcagattt aatgcatcac
    27961 ttaaattttg ataagtaata tttgacatta ataaattaaa tttcccttga gtcattgaca
    28021 aatatgttag tcctgcagga ggaatgtttg catttccgtt atatggaatt atgtcattca
    28081 tatcttgatc aatgtcgttt aaattaacac tctgggcggc aaatattttt gattttgact
    28141 tatcggctaa gcttattttt tgattattat aaataacaaa gaccgaaaag cctattatag
    28201 ctataacaca agccaataaa cttatttcaa ttatattttg tgcttttctt ttcatacttt
    28261 ctccttaaat tagaccatat agctttataa tgcccagttt ggcgaaattt ataacatatt
    28321 ttacaacatt ttttacaatt atgcaaaatg ttaatatatc atattaatca ttaaattcag
    28381 atatgaaaat ttttacccat ttcatcagtg atgaaagctg atacggttta gggatataaa
    28441 gctctgcgcc tgtatttaat actaattctt tatcgtgcag aactgatgta ataattattt
    28501 ttgaattttt aaatttagta ttttctttta agtatttgca gatatttaag ccggaaaggg
    28561 tttcaatatc gcctgcatca attataataa ccgcaggcgg ggtgtcaact gttatgtctg
    28621 tcgataaaag agttctcatg gcttgaggat ttttaagtaa ttgtgtttca taaccttcca
    28681 agtcaaggtt tgtagccaat aaaaaagaaa gtgcttcatc ttcttccatt acccagattt
    28741 tgttgttaaa atttttatct agaaccgcat tttgcgcgct tattttgggt ctttccactg
    28801 catagcttga cccgtaaggt ttttttgcaa gattatgcac ctgtaataga gaatttataa
    28861 tctgttttac atcagtgtat tttttaaact cattgcttgt aatgccgatt gtgctaaata
    28921 caaaatcaga gcgcctgcca gcaagttcat ccccctgaag tattgtgtag ccgcggttta
    28981 catcctgagt agaataaaat ttttgagata tggaatcaaa tgcaaaagtg agatatgaag
    29041 cgattttttc tgccctgata gggtgtgtta ttatcaaaaa ttcattttcg gaaaaatgcc
    29101 cgaaataatc ttctttttcc aacgcagatt taataatagc gcaataagtt tgaataagtt
    29161 tatctgaagc aagttctgaa taaatttctt tgtaattttc aaaattttgt acgcttatta
    29221 aaagacatgc ccatttagaa tcctttgaaa tgattctttt cagggctttc aatgaataat
    29281 ttttattggg aagcattgtt tttaaattaa tattagattc atgttcgcgt cttaaatgcg
    29341 ctttaattct ggttttaaat tcttctgaat ttataggctc gcttaaaaaa tcgtcagcac
    29401 cgttttcaag agcattaatt ttatcttctg tttcggcgga ttttgacact gcaactattg
    29461 cagggcgcat gttgtaggtt aaaattctta gttttttgca aaaatccgac agtttttctt
    29521 ctatgctgtc tgaaattata atcaagtcag gttcgttgtc ttgaattagt tttattgccg
    29581 aattcaagct gcgagaaata agtacaatat tctcatcatt ttctatgagt tttttgtact
    29641 ttgtcgaaag ttcttttcgt ttgtctataa tcaaagtggt tttttgcatt tttacctttt
    29701 tatcttttct ctcccccctc gcgaaattgg cttatttact tttttatttt tcccctctcc
    29761 ccttgcggga gagggctagg gtgaggggtt ttaagaacat attttggatt gcttcgggct
    29821 tcgccctttc aatgacattt atttattatt ttcacatcct ttaaattgct ttcctgcttg
    29881 attttcgaga ttttctgttg ggattaatat ttcaaaaaca ttaccctcgt cgctgcccgt
    29941 aacatttatt ttcccgttca ttttttctgt cagcactttt gtaatataaa gacctagccc
    30001 gcttccttga acttttctag tgagcggatt gtcgagtctt gaaaatttcg tgaaaatttt
    30061 gtcgtaatct tcttgattta tactgattcc ctcatcaatt acttttatgg aaagataatc
    30121 agggcaaggg gaaaaatcag cttttacaat tatttttgtt ccttcgttgg aatattttgc
    30181 cgcattttcg attaaatttg tcataatttg ttggaattta tccacatcaa caaaaatttt
    30241 aggaattttg gggttgaaat ccgtttgaaa ttcgtgctgg ggatactggt gttttacgat
    30301 ttgaataatg ggctcaatta atgtgcttac gttagcgttt ttaaaaataa aatcgtcttt
    30361 aaaagattga agtttattga cagaaagcag attttcaaca agattaataa gcctgtttga
    30421 ttgctgttct atgatttcga taaacttttt tttgttgtca tcatcgagtg catcccagga
    30481 attaagcatg gtttgggcaa accctcttat actggttaag ggagttctaa gctcatgtga
    30541 aactgtagaa ataaaatcct gctgtttttg attttcgtcc atagctttat tataacaaag
    30601 ctttcaaaaa caacaggatt tattaagatt ttttttatta ttcgagatta tttaatacgc
    30661 ggatttccag gttttatatt tattccagct atcaatctac gataatcttc tggggtgacc
    30721 ggtttattgc tatctttaca tggcaattct tccgaagagc tacctcccga agggttttgt
    30781 tttcgtgtag gtagatacgc tggattaaat ccgccgattc taatactcat aatttactcc
    30841 tttcaattag ttaaacgata aattaatatc ccttgtatta catattcaag ttgtcggagt
    30901 gttttataaa aaactttaga actttgattt aaattgtaat attttgttac aaatgaatat
    30961 tgaaaattgg gcagaaaata tttgtcgtta ttgctttaca taggtctgca aagttttatc
    31021 ataattgaag aaacgaagca atcttgtctc tcattgttaa gacttgtagg agattgcttc
    31081 ggtgggtaat agtcattaaa gccgataatt cccgtctcgc tcttgtaaca catattgaat
    31141 atgtcggagt aatctgaaaa aatatttaat aatttcaaga aaaatttaac aaattgttac
    31201 aaggggtttg aaaatttaat ttagtaaaaa acaaaaccct gctgtttttt aaaacagcag
    31261 ggtttattaa gtttttttat tattctgtat aaattttacg attaatcccc gcttctaatc
    31321 cagactaact ttacaacatg accatcttta tctttaacaa ttattgtggt accgcctttt
    31381 acttcaggga tgtctacttc actaatggct tgttctagtg gcagcggctt aggggtaaca
    31441 tttctttcag ttgaaatttc aactgttcct ccctcaggca catcaataat aatatcatcc
    31501 cacggaggat tatgaataat aatcgtagac ttagtctcca ttacaccagt atcaggtatt
    31561 gtaacaccct ctataccttt agccagtaaa ctatctttta atctggtcaa ctttgaattt
    31621 tttggatcaa gagatatttg ttcgtcaagt tttttcataa atttctcgaa ggcttcttct
    31681 tttttcgatc cttctaagtt tttacctatc tctttatctg atggttttac aactgttcca
    31741 agagccgctg cctgtgcttt cataaaagca tcaacagtac ttgggtcaac ttttgctctt
    31801 tcaggtgcta cctgtggcgc tgtttctttc ttttcagctt catctttttt ctttggtcct
    31861 tgagcgtttg gatcaattcc tttaaaacct tgcatcctaa catctgtcat aaaaaactcc
    31921 tttcttaatt ctaagttttt ttaagcattt ttaatactca cattttaaac ttttttttgg
    31981 ggactgtcaa tattgaaagt agtatatact atcaatttcg agtgtttcaa atattttgtg
    32041 aagacaaaca agagcctctt ttgtaaggtt tgtaacctct atattaattt ttgctctttt
    32101 gttttaaaac attgaagggc ttgacactcc atcctttcta atgaataatt tcggggtaac
    32161 tgtaatgctt aataaatata ttcggtaaca ttagaataat actttagagt ttttattata
    32221 atgttaacaa gttgttacat tgataaataa atcttagttg acttgtcttt ttaaattata
    32281 ctttcgtatg gattcgtatt ttaaatttta ttaatagagg atagattgct tcggtgggca
    32341 aatagtcgtc aaagccgata agcctcatct cgcaatgacg tgagtggctt tagcaaaggg
    32401 ttaaagcata tttacccccg caatgatgta aaaagggatt cttcgggctt cgtcctcatt
    32461 cttgccaaaa tccgcctcag aatgatgaat gtgctgccct ttcaggatga ctgcgtttta
    32521 tttgtgcacc gcagggggtt gaatatatag cggtaaaagc tttttccaat cggtgtcaaa
    32581 atcagtattt aatttttcct gagcaatttc tgttaaaaat ctgcccaaat catagtcatc
    32641 tttttgataa gaaattccgc ccaaaacgtt ttgaagtttg tcgtcggtaa tcacaaaata
    32701 ttcttctttt ttaatcattt cttttaaatc ttcaagaaac atagcctgag gaggtaaaat
    32761 ttccttttta ttatcatata tcgcaacata agccgtttct tttctggcat caagcgcaac
    32821 gagggtaggt ttattccctc gccccttgcg ctgagaattg taaaaataat cgctctccct
    32881 ctccccttgc ggtaaggatt gtaaaaataa ttgttctccc tcgccccttg cgggagaggg
    32941 ctggggtgag gggtattgta aggcaagagg gtaggggtga ggggtattaa gttcattatg
    33001 taagaattca aaacagcctt caatgttttc attgatttca ttattccaaa atcttattat
    33061 tctaaaatct tcgttttcta tataaaatgt tctttcaata tcagaaaatg agccattatg
    33121 ttgtcctccg tcaatttcaa taatcatccg tttttctaaa caaacaaaat cagcaatata
    33181 tttattatta atagaaaatt gtcttctgaa tttaaaacca agtttattat ttcttaaaat
    33241 tgaccataat tttgtttctt gtggtgttgc attttttctc atttttctag caaattcttt
    33301 tatgtatggg gcatagtttc tatgtgcgat tttatacccc tcacccgaat ttctgacact
    33361 cgcaggctcg tgttgaaatt cgccctctcc cgcaaggggc gagggaaaag tgacggatgt
    33421 caaatcattt accccctctc ccgcaagggg cgaggtttgg gaatttattc ttgacaaaat
    33481 ttctaaagat gaaacaccca ctgctttttt atttaattgt tgggcataaa ttctggcaac
    33541 tgtcgtgcag gctcttatgc ccgtaaagct tccgggtccg atatttgtta cgattacatc
    33601 aatatccttt ggagttaagt tattttcttt taaaaacttt ttaattgtag agattaaaaa
    33661 agccgaatga taatgttttt cggttgtttc aacaattttt gagggaataa ttttatcctt
    33721 aacacgcccc ccgatataca ttttatccaa gcatgtatca aaagccagta tattcatttt
    33781 aagccctcaa taatttttat attttcttgt ccgaaagctt caaatgagta ttttctagcg
    33841 tcatgtttat atccctcact cgaatttctg cttacggtac ccctcacccc aaccctctcc
    33901 cgcaaggggc gagggggaat gagttgacat gaatttaagt tacaattatc tacatattca
    33961 acgttaattt ttatatgatt aaatgggatt tcgtcttgtg aaaattctgc ccattcgaca
    34021 aaagttaaaa tctttccttc tgaatattca cgtaattcat ccaaaatggt ttttacgcct
    34081 tcgttttcca gtctgtataa atcgaagtga taaatcggaa gtttgccgct tttatattca
    34141 tttaaaatta caaaactggg gctggtcact ctttctttga cattaagtgc ttttgcgaca
    34201 agttttacaa aagcggtttt gcctgctccg atttgaccgt aaaggcttac aaaagcacct
    34261 ttgtctttaa cggcaagcgc aaacttttgg gctaattttt ccgtatcttc tagtgtttta
    34321 catgttattt ccataatttt aatgatagca tgaagacaat ttaggttgaa atccgctttc
    34381 ctttgcttta aaatactgtc atgattgaag aaaaagtttg ttacctgtgc ggaaataaag
    34441 attttgaaaa gcgtgaaggg aatgtcaggg ataatcccga cttaaaagtg cttgaatgca
    34501 agaactgttc gcttgttgtg ctttcttctt tcgatcacat taatgataaa ttctatgaaa
    34561 acgacggtat gcatggcgga agtcctgatg tccaaaaatg gcttaatgcg acaaaagacg
    34621 atgataaaag acgttttcgc cttttgaaaa acgagattaa agggaaaaaa gttctggatt
    34681 ttggttgcgg taacggcgga tttttgagct tcgctcaaaa atccgcccga aaagctgtcg
    34741 gggtcgaact tcaaaaatca atgcaggaat attataactc tcaaaattta gaagtttatg
    34801 aaagtattga ccaagttagt gaaaaatttg attatattac ggtttttcat gtacttgaac
    34861 acataaaaaa cccgattgtt ctgcttgaaa agttatctga aaaactcaat gagggcggta
    34921 aaattataat agaagtgcca aattcaaacg acgcgctttt gactgtttac aaaaacaatg
    34981 cgtttatgaa ttttacttat tggagctgtc atttattttt atttaatgca aaaaatctta
    35041 tgaatcttgc acaaaaagcc gaatttaata ttaattacat taaacatgtt caaagatacg
    35101 gatttgcaaa ccatttaggc tggcagattt tcaaaaagcc cggcggacac aaaaaaatgt
    35161 ggtatttaaa cattggattt ttaaataagt tttacgaaaa atcgcttgcg cagattgaag
    35221 ctacggatac gattttaatg agtatttcaa agtaaatcag tattcttcac attttttttt
    35281 gtttttgtta aactaaatca agtaggtatc tcataatacc aaaataaaca gatcccgcaa
    35341 ctagtgcggg atgacaatgt ttaacataga aagtaagtag gttgggcttt ctagcccaac
    35401 gagattgatt tagaaagaga aaaatgatta caaaatataa aactaaaaac tgcggtgaat
    35461 taaacttaaa aaatattgac gaagaaatta ccttgtcagg gtgggctgct tgtattaggg
    35521 atttgggcgg aattattttc gttgaattaa gagacagaag cggctttttt caggttgttg
    35581 cagatcctca ggttaatccg caggtgcaca aagtttttga acatttaaaa acagaaacgg
    35641 ttttgactgt taaaggtaaa attacaagac gccctgaaga aacttataat gaaaaatatg
    35701 caaccggtca ggttgagatg tatccgacgt ttgttgaggt gctctctgag gcaaaagttt
    35761 taccttttat gcttgatgat gagaatgtat ccgaagatgt aaggcttaaa taccgctatc
    35821 ttgacataag acgcgagcaa acgcttaata atttaatttt gcgccacaaa atcgttaccg
    35881 cgacaagaaa ttatttgaac ggacaggatt ttcttgaaat agaaactccg attttaatta
    35941 atacgacacc tgaaggtgca agagattact tggttccttc aagagtgcat gagggaaaat
    36001 tttacgcact tccgcaatcg cctcaaattt ttaaacagct tttaatggtg ggcggggttg
    36061 aaaaatatta ccagatagca aaatgtttca gggatgaaga tttaagggca gacagacagc
    36121 ctgaattcac tcagatagac ttagaaatgt cttttgtaga acagcaggat ataattaatg
    36181 ttgtagaagg aattttggtt gatgcgttta aagctgcagg agttgaaatt aagcctcctt
    36241 tcattcagat gacatacaag gaagcagtag aacgtttcgg ttcggacagg cccgacacac
    36301 gctttggatt agaacttttt gatgtgacag atattatgga agcttctact tttgaagcgt
    36361 tcaaaggagt tattgcaggc ggcggtacgg ttcgcgcgat aaaaatcccg ggcattgcaa
    36421 attattcaag aaaagatatg gatgatatca gaaatcttgc aatttctttc ggtgcaaaag
    36481 gacttgcgtg ggtaacttac atggaagacg gcgaggtcaa atcgcccgta ttcaagtttt
    36541 taaccgaaga ccagattgca gacattcaaa aaagagcaga cgcgcaaaga ggcgatattg
    36601 ttttctttgt agcagataag ccgaaaattg tttttgatgt tttgggaaga ttcagacttt
    36661 atttcggtaa aaaacttgaa atgattgatg agaatcagca caatttgctt tgggttgttg
    36721 attttccaat gtttgaatac tcggaagaac atcaaaggta tgtttctgtt caccatccgt
    36781 ttacggcacc aaaccctgaa gacattgaca aaatggaatc agaccctgct aattgccgtt
    36841 caatcgccta tgacattgtc tataacggaa cagaactcgg tggaggcagc gttagaattc
    36901 attcgggcga attacaggaa agagtcttta aggctctagg ctttacagaa gaagaagtta
    36961 aacaaaaatt cagctttatg gtaaatgctt ttaagtacgg aacgccgcct catgcaggtc
    37021 ttgcaatcgg tttggacagg cttgttgcac ttcttgcggg aacgacttca atcagagatg
    37081 taattgcatt tcctaaaaat tctaccgcaa aatgtcttat gacggatgct ccgacagatg
    37141 cttcaatcat gcagcttcgt gaattgcatt taaaaagtac ggttgtaaag cattaaaagt
    37201 aggtcggcat tgctatgccg acaaaacaag tcaataaata gcgggatttt tctgaaagaa
    37261 aaatcccgct atttatttca tattgtttgt tttcgatgga ctgaatccaa gtctatgttc
    37321 ttgccatttt acagtagtag gtcaagcacc gcttgacaaa ggtaaagtca ggcaatgctt
    37381 gacttactga atatttggtg ttatgactag cttttagcta ttttaaattc gtatttacaa
    37441 aagttagtga ttgacttttg gttggtttta agctacacta tcttaatgaa agaaattata
    37501 acttttgaat tgaataattg caaagttcct tttgaagatt ggctttgtaa attagacaat
    37561 acagtcaaaa gacgaattgc ccaaagactt gccaggctag aagaggatga ttattatggt
    37621 gattgcaaag caattgataa tgaaattaaa gaactaagat ttcaattcgg ttcaggttac
    37681 agaatttatt ttcatgaaac aaacaatata ataattcttt tgctttgcgg cggtgataaa
    37741 tctactcaaa gtaaggatgt acaaaaggct aaagattacc ttaaaatttg gaggaaacaa
    37801 aattatggaa cgtaagttta aaaattcaat atcgtgggaa gaagtattaa taagacaact
    37861 tcaagatgaa gaatttgcaa gagattttct taatgatagc ttcatggaat acgttagtgg
    37921 cggtaatttt aatatgtttt tcagatcgct tgaaagagtt atcaaagcaa gaatgtctgt
    37981 aagacaattt gcaaaagagg ctgaaattga cagaaccaat ttatacgact tattcaaagg
    38041 aaaaaagaaa cctcagctta aaacggttat aaaaattctt gcaaaactcg gttatactct
    38101 taaaattgca taaaattatg aaaatattta atgaattaaa tgaaaataaa gggctgtcat
    38161 tggcttttgg tttttttgac ggggttcacc tcggtcatca agccgtaatt gaaagtgccg
    38221 tgtcttatgc aaaggaaaat gacaagaagt ctgctataat tacttttaaa aatcatcctt
    38281 gctgtttttt taatcatatt aagccaaaat acattacaaa aatatcagac aaatttttac
    38341 tttttgaaaa acttggggtc gattatgttt attgccttga ttttgatgag catttagctc
    38401 ttatgagtgc ttctgattac ttaaaggaaa ttataataaa ttattttgag ccctgtgcca
    38461 tttcaacggg ttttaaccac ttttttggag cggaaaaatc cggaaatgtt aaatttcttg
    38521 ccaaaatgca ggaatcattt gcttataaat atattgaagt tccgcctgtg ctttttaaca
    38581 atgaggttat cagcagtaca agaatcagag aaaacatagc cattggtaac atcgagcttg
    38641 ttaattcaat gcttggattt gagtattcta ttgaagaaac agttattaaa ggtcgtcaaa
    38701 ttggcggaac tatcgggttc aaaacggcta atctggttta tccaaaagat atgttagagc
    38761 ctgctttcgg ggtttattgt gttaatgttg attatgaaag taaaatgtat aaggggattg
    38821 ctaattttgg cttaagacca accgttgata atactattca cccaatattg gaagttcata
    38881 tcctggattt tgataaggag atttacggtg aaaaaattcg ggtacatttt ttgaaaaaaa
    38941 tccgcgacga aaaaaagttt ggctctttgg atgagctgaa agcacaaatc caaaaagata
    39001 tttctgcttt aaattaattt cggacagtat ttttcaatcg ggcattggtc gcatttcgga
    39061 ttaatcggct tacaaacatt ctgcccgtgg gttaccataa gggtgtttat ttctatccaa
    39121 tatttttcgg gaagcttttt ccttagcgca aattctgttt cttcgggggt tttagtttta
    39181 aaatatccta agcggttaaa aattctgtga acgtgaacat caacacatat tgcaggcttg
    39241 ttaaaaccct ttgccaaaac aagattggcg gtttttctgc cgactccttt gaatttaata
    39301 agttcttcaa tggtatcggg gacttttgaa ttgtattttt cgactaaaat ttttgataat
    39361 tcaataattt gttctgcttt attcttgtaa aacccgacag gataaatcgc ttttcttaat
    39421 acttcagggc ttatgtgggc gaacgccgca ggcgttcgcc ccagttctaa cattctcaaa
    39481 gtcgcagggt aagtcgttct gtcgtttgtt cttaagctta aaatacaaga aattagtacc
    39541 aaaaacggat tgttaaaact gtccataagt tttacaaact cgctttgggg ctgttttgct
    39601 ttttttaaag tttctactat tttatcaatt tccattaatc agtttttcca catccaaatt
    39661 tgttttcaat tttagcgcga aaactttaag ataaaacatt gattggtcaa gctttgtcgc
    39721 atctttttcg gtggtgacaa gtttattgat attcatatcg atgcattttt tttgcatttt
    39781 ttgaacatca tttttggaat atttatggtg atcgtcaaag ttaaaggtat ttacaacgtc
    39841 aaaatttttc aaaaaattat aaaactgctg aggctgtcct atcgcgctga aagcaacaat
    39901 tctatcaccg ttttcaagtt tctcgttcgg ttcaaaaata ttgtaagcaa aatcaggttc
    39961 gctgtaacaa agagaagtcg gtttattcaa ttttttctcc aaaatgcgtg caaatttttc
    40021 tgcccttgta tggtcaatgt ttttacttac aataacaagc ctgtcgattc tttcaaaggc
    40081 ttctgttccc tccctcagcg gacctgcggg aagtaggttt tcgttgccga atcccatttc
    40141 gctgtcaacc agaacaatat ctaaatcccg atgaagtttt ctatgctgaa atccgtcatc
    40201 aagaattatt tttgtggcgc aaaatttgtc tattgcaaat tttgcgcctt tatatctatt
    40261 ttttgctgtg atgactattg cttttgtatt ttgcgcaagc caatgaggtt cgtctccggc
    40321 ttttcttgcg ctgtagtaaa tattttcacc gtctgaaata acatttactc ccgataattt
    40381 tccgccgtag cctcttgata atattgcaac tttttcgcct ttttcaatca aataatcggc
    40441 aatttgggca acaacaggag tctttcccac gccgcctgtg gttaaatttc ctactgaaat
    40501 aacataagcg tcaacttttt taggctttaa tttatctttt tcgtacaggc tgtttttcag
    40561 tctgcttgca aagccgtaaa acagagaaaa gaattttaga aggctgaaaa acagcccttt
    40621 tgcctgtttg ttgtagtgga ttttagttat ataagttttt aagcccatta atgttttacc
    40681 cctcaccctg ccctctcccg caagggggcg agggtaatga tatttttgat tacctttatc
    40741 ttaaatttag aacatcaatc taaagagtaa aaatcttttg tttaatttat cgctaaattt
    40801 tcttaaatta tgggttgtcg cagatgtatc ttccataatt tgttcgattt tgcgtttatt
    40861 ttcaggtgtc attgaattaa caagttccat tgcgcttgaa atatcagtca ttgctgtatt
    40921 aacttttaaa atagtatcgg aaagttgttt tttaagcttt tcgtcttttg tcattgttga
    40981 gacaaaagtg cttatgtcgt taaggttatt ggcaatcgat tgtaaatctg cgccgagttg
    41041 tttggcgtct gtgttatcaa gaattttgtt taaattttgt gatagtttat caattgattt
    41101 tgctgttgat ataagagtag gcttgaagtt ttcatctcca actacgccat taatgttatc
    41161 cgcaagttta ataaagctgg tgcttacttt atttgccata gcgaccattg agtctaagtc
    41221 ttcttttgaa gcatcaacaa gtttctcggc cttggctatt gtggtcgttg cctgtgcggt
    41281 aagcgcgttg atatttgaca ctgtttgttt taaatcggca ataacgttat cggaaagcag
    41341 ttcgtttatt ttaatatttg tttccgtgag agattctgcg gctttatatt gcaagtccat
    41401 aaaatcggca attctcatag gttcaacaac agtgtaagga gagctttgat gcggataagg
    41461 caaaacagtg ttgtcaatca tttgtaagcg aagtttgttt acacctttgt cttttatcat
    41521 actgccgagc atgtaatctg gcataataag ccccggaagt gttattacgt agtcaatttt
    41581 gtaagcgtaa ggggttttaa ttttttcctg cataatcgcc ggaaccattt tggcagaaag
    41641 aatttgagta tctttaacct tacctacgtc ataatatttg ttatcgagtt taatctgaac
    41701 agggtcattt ttgtaaagca aatcagagcc gccatagacg gaaaaataag ttgtccttgt
    41761 tttaggaggt gtgatttcca agaactgttc accgataagt cctgactgct gaattgaaaa
    41821 tacagaagct ttcggaatgg aaattccggg ttctgtaata acgaatttca ttttaacaaa
    41881 gctgttttca ccgtcaatga agggaataat ttcttcgact tggccgactc ttaagcccat
    41941 catttgaact cctgaacccg gtctcattcc gtttacgtct ttaaacacga tttccatcct
    42001 tgcagcagat gaaaaagaac ggcctttaac ccataaaatc gtgaataaca aaatgaaaag
    42061 ggctattaat gccaaaattc ctactttaaa cgatgatgaa aatctcatat tacctcttcc
    42121 tgttccattg tatcaaaaaa aacttattct gccaacattt gcataggtcc ttccaaagat
    42181 gccgatacga attgccttgc gtaatcatta tctgaagtta acatatcttt aggtgtgcct
    42241 tcatatacta ttttgccgtt ataaagcatt atgattctgt cagccgtacg ctgaattgta
    42301 gacatctggt gagttaccac aatcgatgat gcatgggttt cttctttgag ccttacgatg
    42361 taatcttcaa ttaacgtcga tgaaatcggg tctaagcctg aagtcggctc gtcatataaa
    42421 attgtttgcg gctcggtaac tatggctcgc gcaaagctta cacgtttttg cataccgccc
    42481 gaaagttccg acggatactt tttttcaatt ccttcaagcc cgaccatttt aagtttttgg
    42541 gcaacgattt cttcaagtcc tctctgggag aatttttttc tgaattcttt tctttctttg
    42601 agcgcaaaag caatgttgtt gaatacgttt agggagtcaa acaatgccga atattgaaaa
    42661 accatagcaa catcacccat tgatgtttca atttctccgc tgtcgggttt taaaagttca
    42721 cagataagct ttagaatcgt actttttccc gaaccgctaa agccaacgat agcaaggact
    42781 tcgccgtctg caacttcaaa cgaaacattg tcaattaccg ttttattccc gaaaattttg
    42841 attaattttt taacttttat actcatgttt tatcctttat tgtattacat attcccttta
    42901 aaaaaagaat gccaatgcaa acagcaagtc ccagattact attgcgacaa atgaccagac
    42961 gactgcttta gttgttgcaa taccgactcc ttttgctccg ccttttgcat tatacccgca
    43021 agtgcagctt ataagcgcaa tagtgccgcc gaaagttacc gcttttgcaa ggcaaacgtg
    43081 aatgtctttc atatagagtc cgtaccaaac agaatcaaaa taagctctgt aattgagccc
    43141 ccctgccaaa tttgaagcca ccgctccgcc caaaaccccc acaactgagg cgacgattac
    43201 aacaagcggc atcatcaaaa gtcctgccac tactcttggg acaaaaagat agcgtatcgg
    43261 gtcaactttc agggatttca tggcatcgat ttgttctgta actctcattg ttgcaatctc
    43321 ggcggcaagc gatgagccta tcattgaaat tatggcaaat cccgacatta taacgccgtc
    43381 ttcgcgcacg ataagcatag ttacaagcat tcctacataa tttcctgccc cctgcttaac
    43441 catttctcct gccacctgca ttgcaacgat tattgcagac attcctacta tcgaaagggt
    43501 tatcggaagt gaacttacac caaagcgggc tgattgtgcg ataacttcgt ttttgtcgat
    43561 ttcaagcttc ataatgtacg aaaaagtgga aattgctttt atgctgattt cccccaatgt
    43621 cgtaaaaaag tcgtcaacct gctttgtaaa aagtctgtat agctgatatg tcgccgattt
    43681 tttaaagtat gatacaattt gactgctcat agctaaatta tattaaaaaa aacaactttt
    43741 ggctatttgt taagataaaa ttttgtttaa tatatgcctc ttaactcaaa agattttata
    43801 atttcttctt ctacttcaga ggtatatcct aattcctttt tgtcatcagc agataatgtt
    43861 tcatatatat ctacaatgtt tatccaatcc gtataattaa tttcataatc ttgatcataa
    43921 tgtgttaaga gaggattatg aggatttaaa attttatatg ctgtcattgc aaaattagtt
    43981 ttatatgtgc cgaattcgca acctaaaata aaattgcctt gttttgtaag ttcagaaaat
    44041 ttaacaaact ttttgcaata accagagtca atattgttaa aatcaggagg catttcaatg
    44101 taaaaatatt ctataccttc ttttttacac ttatcttcac cttcataatc atataaagga
    44161 tataatgata ttgcagtttt tactcccagt tttttaagtg tttctaaatt cttcatcgaa
    44221 tcaattattg agcctctgta accgttgttt ttcaatttac caaatgattt tatattgtgt
    44281 tggatgctag aagttaattt ttctatgttt tttagatatt cttcctgttc atattcagac
    44341 tttctgccct cttgataata ttgataattt gatgaagcta aataatcaaa taagtggttt
    44401 cttaaataat tagcgtattt atcatgaaca ggacacatga tatgtccgat tttttcgtca
    44461 tcattgtctg attttttttg atttttacca aacgtcgttt gtttgtttgt ttgtttgttt
    44521 gtttcgtcaa attgatagtg ttaacaagca tagccaatag tttattacct tgatttaatt
    44581 taccattgtt ttaaaacgtg cttttaattt tttttgttat tttgtaatga ataatttaca
    44641 attcttattg attttttatc tcacgatttc gtttttattg ctaaattttt taaccatgtc
    44701 ttcggcaagc aagacggata attcataaac attttgaggc tcgaaaattt tatcaggcga
    44761 gtgaagaact atctgaaagt tttccttatt tgcctgtttg catagaattt caagttcaag
    44821 aaggtttttc ttgaaaattt caacaggtga aatatttttg tcgtttttat attttagtga
    44881 aaagtctttt ttcatctttg atatgacctt ataaaatttg ctatcgaacg gtcataagtc
    44941 ttttctgcgc ttgcggggaa aaaacagccg ggaatttctc cgttttgtct gtgataggca
    45001 acacaatcgc agcatatacc tttttttgaa caggaataag tgcatgtgca gttaatttta
    45061 ttttcttctt ttttacactc cattttttgc tcccatactg tatttacagc cgcagtaatt
    45121 ttgtctgtaa agtcctaatt cttttgcttt ttggttagac tttaaaaagc cgtcattttt
    45181 cttaaaatca atgtccaaat aatttatacc atatttgttg gaaattgtct ttccgagttt
    45241 ggaaatgagt tcaaagcttt tatgagggct tataactatc gttgttgtaa agctttttat
    45301 gccgagttct tttgcttttt tggcgctttg tgtgagcctc atttcaaagc attttgcgca
    45361 tcttttgccc ttttcgggct catcttccaa tccgaatgaa ataatttcgt ggtattttgg
    45421 ttcatagctt tcaataatta actcgcagtt taaattttta caaagttcct tttgcgcacc
    45481 gagtcttttg ttaaattcgc attcgggaaa aatattgggg ttgaaaaagt atacaacagg
    45541 ttcatatccg agttctctta agtgtgaaat agggtatgcc gaacaaattg cacaacagct
    45601 gtgcagcaag attttattat cttttttcaa ccgcgcctgc ctttataagc atttctttaa
    45661 attcgtaata tggttttatt ccgggcataa ttttgccgtt cataaccatt gtgggtgttc
    45721 cgattattcc taaatcaatt gaattttcta tgtctttttg aattctgtcc gaggtgtctt
    45781 ttgaattagc gtcttgtctt aatcttatag tgtcatagcc caatgatttt gcaagtttta
    45841 gaatgtcgtc ttcggttttg ggctgttttt caaaaagctc cgaattgaag tcccataaat
    45901 gtccttgatt ttgtgcggct acggaatatt tagcaagcat acatgcacct tcgtggaact
    45961 cttgtcttac ataatggtta cactcttcat caagcggcag gttgtgatga ataaatttta
    46021 tattacctaa ttcttttgcc attctgtaaa ccataatatt gaatgtgtag caaatgggac
    46081 agcggtaatc tgtgtaaaca tctatgataa tttttgcgtt tttatcgcct aaggtgtttc
    46141 cgcctgactt aaacggattg gttttcatat ccgcaaatga ttttatggat ttataccttt
    46201 taacctgcgg ggctaaaaca taacttgtag cggtataggc aagaactccg cctgcaatta
    46261 atgccagaac aataaagctg attaaatatt tttttattct taaagcatga ataaaatcaa
    46321 ccgtactgat tttaaaactg tgataaaaac cggctttccc gacagctgcg tatgctataa
    46381 aaagatttaa aaaataagta aaaacacaca gtatgcatat ttttttgatt tcaaaaatgc
    46441 ttatacttgc aagtatcatg gaaattaaaa aagaaattaa ccccaaagcg cagatgtatc
    46501 tcatcggatg tttgaaaact tttaaaaaat gtaaaaattt aacgtgtttc agcttgttta
    46561 caaaaagtag aaaaatcgta aaaatataaa acagcaaccc ccaaagcgca agaggaattc
    46621 ctaaaaactg tgaatgaacc gtttgtgcaa cgccgtcgca atctacaaat tggtttattg
    46681 aacaaaaact cggaagcgca taaggattaa agtttgaatc ataatatatc accgataact
    46741 ttacagttgt ggcaaaacct attactgcaa gaataaatat tacaattttt ctgacaaccg
    46801 ttacatttat gttaaacatg tattactccc taaacctttc ttttatacta actgtaaaaa
    46861 aatttttttc aagagtttat atgacaaatg ttttgtgttt taattgaatt acaaagagat
    46921 tgaggttttt atgaaaaaaa taaaaattgg tatgattggt ttaggaactg taggttcggg
    46981 tgtttttaag acgctgtctg attttgaaaa tattgaagtg gtaaaaattg cagtaaaaaa
    47041 tttaaaaaaa caaaggaatc ttccaaaatt aaatgaagcc attttaaccg acaatcctta
    47101 tgaagttgtt aaacatcccg atattgacat tattgttgaa gttgtaggcg gaatagaacc
    47161 tgcttttgat ttgattaaaa cagcaatttt aaacgggaaa cacgttgtaa cggcgaacaa
    47221 agaactgctt gccaaacacg gcgaagaatt atttaatttt gccgaagaac ataacaaagt
    47281 tgttctctat gaagcggcaa tcgctggcgg aattcctatt attatgccga ttaaaacaat
    47341 tttagcggga aataaaatta caaaaatcgc aggaatatta aacggaacaa caaattacat
    47401 tttaactaaa atggatgttc agggtgcttc ttatgagagc gtcttaaaag aggcacagga
    47461 gctcggatat gcagaaactg accccacagg ggatgtcgaa ggcttcgatg cggcttataa
    47521 gcttgcgacg cttgccacaa tagcttttaa taaaagaatt aacataaaaa atatttacag
    47581 agaaggcatc agcaaaattc aggcagaaga tatgattttt gcaaatgatt tcggctataa
    47641 aatcaaactt atcgcacttg caaagcttga cgaaaaagga aacgcagatg taagagttca
    47701 tccgatgctt gtaggtaaat caaaaagcct tgcacacatt gattatgtga caaacgcggt
    47761 tgaaattacg ggtcatcccg tgggcgagat tatgctttca ggtcccggtg cgggtgaata
    47821 tccaacggca agttctgttg tcggtgatgt tcttgcgatt gcaacagaaa taggaaaaac
    47881 cgattattta ttgccgatga tgagatgcca ccaccacgaa actgctaata tgctcaatat
    47941 ttcggacact tacaataaat attatctgtc tgtcaacgtt aagaataatt tcggtgtaat
    48001 tgccgctatg ggaagcgttt ttgcaaaaaa taacataagt atttctacac tcttgcaaaa
    48061 aggtttaaaa agcgacaata ccgcaaatat aattgtaatt accgaaaaat gccttgaaaa
    48121 agacatgcaa aatgcgatta aagcttttga aaaagatact tgtatcaata agattaacag
    48181 tttaataaga gttatggagg ggtaaatgtc aaatcactat agaggattaa ttgacaaata
    48241 caaagagtat ttacctgtaa gtgataaaac tcctgtaatt acccttaacg aaggcaatac
    48301 gcctttgatt aaagcagata atcttgcaaa aaaaatcgga attaattctg agatttatct
    48361 gaaatttgaa ggagcaaacc cgacgggaag tttcaaagac agaggcatga caatggcagt
    48421 ttccaaggcg gcagaagccg gctcaaatgc cataatctgc gcctcaaccg gaaacacttc
    48481 tgcttctgcc gccgcttacg gggcaaaagc aggaatgcgc acttttgttt taatcccgga
    48541 cggctacatt gctttgggta aactttctca ggcgatgatg tacggggctg aaatcatagc
    48601 gattaaaggc aattttgacg aagcacttga agtcgttata caaattgcgg ataaatatcc
    48661 tgtaactctt gtaaattcgg ttaatcctta taggattgaa ggtcaaaaga caggagcgtt
    48721 tgaaatttgc gaagctttgg atgatgctcc cgattatcat tttattcctg tcggaaatgc
    48781 aggaaacata accgcttact ggaaaggtta caaagagtat tttgagttta aaaaaacaac
    48841 taaaactccc aaaatgatgg gatttgaagc cgaaggagca gcgtctatag taaaaggcga
    48901 gagaatttta aaacccgaaa ctattgcaac tgcaataagg ataggaaatc ccgcaagctg
    48961 gaaacaggct gaagatgcac gcaatgaaag cggcggggta ataaattttg ttacagatga
    49021 tgaaataatt tatgcataca aattaatcgc gtcaacagaa ggaattcttg cagagccagc
    49081 aagtgcggca tctgtggcag gagtcataaa agcaaaaaaa ctcgggttga ttgaagaagg
    49141 ttcaaaaatt gtgtgtattt tgaccggaaa cgggcttaaa gaccctgatt ctgccattaa
    49201 atactcggct tcacaggtta aaaagacatc atctcaaatt ggtgatatac taaaggtaat
    49261 gaatatataa ggggttaatg tgctggggtt tctgtttgct aaaaaggaaa taataaaaaa
    49321 agatttcgaa ttaaatcaaa tttatctgaa attaaaagaa atttataaat ttgatgagat
    49381 ttctgatgac agaaaagctt ttctgcttga tttgacggaa aaatatcaat acttgcctta
    49441 cccccatata aaagctttag aagaactttc tgatgcggaa gttttattcg cacttgaagt
    49501 aaaatgggga cttaacggaa tatttaaaaa cggagaattt acttttgaaa atgataaaat
    49561 aagcccgctt tcaagaaatg atattaaaaa ttcaaactgg attaaacgcg aaggtcatga
    49621 cataaagctt gtaaatcttg cggggcttgg aaacggcaat aagtatgata atgcagggaa
    49681 atttattgat tggcttcgtc agcttttgat tttgcctgcg ggtaatattt cgaaaggaat
    49741 ttttcctaca acaatttatt tgattccttt tcacccgaga gaattcggct gtgcttatct
    49801 gcccacaagt accgacgtga gcccgaattt agaagatgaa acattaaaaa tagaactcgg
    49861 attagatgca aaagcgcagg tgcagacttt tattacttta gcgcaacttg cggggcatcc
    49921 tgttatttat gatgtacttc cgcagacggg cagattttca aaattagttt tggcaaatcc
    49981 ttttatcgca aggtggtttg atataaacga attgcttgct aaacttgaaa attccgctga
    50041 tttagtcgcc caaaaacttg aagaaaaatt cgataaagaa gatattgaag ttataaaaga
    50101 tatctgtaaa caaacgctaa aaagcggggc gggtgattta agcgagcatt atcagggaat
    50161 ttatgatgaa tttgaagccg aattgattga acttaaaaaa cagttttcgc aggaaatgtc
    50221 gatgaaagaa aatcagatta agctacaaaa gcgagcaaaa gaaataattg caggcataca
    50281 tgagcaaaag tcttcaaaaa attttaatga acatgaaatt acaaatcagg cagattgtat
    50341 aaaaggatta atggcagaag gtttatggcc gctttccggc ggtgcatggt gttctgctgg
    50401 agttcctgtt tttgacagaa tgagcgactg cggaagttat ccgatgtttt tgcactttga
    50461 ttttaacgga gaagatgttt caaattttgc caaccttgac tgccaaactc cttattactt
    50521 tacttttttg gaaaacggtc aattaaatgt ccctgttgtt gagttttttg ttaatcttat
    50581 gaaaaaattt caggaagatt ataattttga cggatttaga gttgatcata tagatcacat
    50641 tgttgatgag gtttcacaaa aaaatgatat tccgataagt tatagagcac ctaaagaagt
    50701 tttgtgcagg cttaatcatg ctatgaaaca aaaagttcct tattttgcca ctatggcaga
    50761 atatatgctc tgggataatt atttaaaaga atatcacgaa gatatgtgtt ttgacttgct
    50821 ttggggaaat gatattattt cgcagtctga aaaaacccct gaaaaaatta ttgaagataa
    50881 tcaaaacctt gccatttaca atacaaaaaa tttcagcatt gaaaatttat ccattctgaa
    50941 aacttataac aatcaggacg gcgagttcag ggtaattgac caatatccgg gacaattagg
    51001 tgaaagcggc gcgctgttta agtggtttaa gtataaattt ttacccggcg gtaaatatgc
    51061 ccaaagaccg gttttatata ttgacgggga tgaatccttt acccaaaaag gtattgagcg
    51121 cgctataggt accgaagttt cgatgaaacg tgaaaaaaat tatgattttt ttgcaaaatt
    51181 cgatgcaatt aaccgttttt ccaaatcaca ggaactgatt ttcgaaggcg aagcacagat
    51241 tataagacag gatgaagacg gttttgtttg ctggcttata tccaaagaag ctgttaaaaa
    51301 tgctttttta attgtggcaa attatttttg cgagacagaa aaaatagccg taaaagactt
    51361 aaaagacggt actcatattg aattgagaga aggcacggaa gtttttgata aatcgcttgt
    51421 tttgccttgt gattatgtta ttaaatcgga atttgtgttt gacggaaaag attttgtgga
    51481 aaaagaactt gggcataaaa caaattcgct cagttttaat gttataaaac cgagcgaatt
    51541 taaaatattt ttattgaaaa aataatccta tttcctttta gctgcttttt cggctctttt
    51601 tgctcgcatt ttagcttttt gttgcacaat atatgatgtt gatttttttg tatctttatt
    51661 ttcaactttc acttttttgc tgaatctttt accgacattt tttaatttat ttaattcctt
    51721 aacagcttca tcttttagtt tattggcttc tgcgattgct tcgtctttta gtttaatgga
    51781 ttctgcgatt gcttcatctt ttagtttaat ggattcatcc atgctttttt tcgctaattt
    51841 ttcaaagccg cctaacagca atttaaaacc ttttgcaggg ctgcctttgc ctgcaatcat
    51901 tattgctgct gcgagtgtta caatcgcacc ccaaaagaaa ccttttcctg tgctgccatg
    51961 tttattaggt ttttcttctg tgtttggtgt tgtttcaaca ggttttgtac ttgttgctcc
    52021 atcttgagag aatttttgcg gttgagatat taatgcttgt aagtcatttg ctctaaatgt
    52081 aacgttcatg tatttctcct ttcaataagt gtctatataa aagcgcgtaa aaatattttt
    52141 tgctcttttt tgcctaaata ttaagaaaat ttaagaacaa attttgcaat caggattttt
    52201 agataaattt attgttctaa atttcatatt taatccgtca taagataaaa gtttattttc
    52261 aaggttttct cctaagttta gaataatttt aacggcttcc atagcctgaa tagaggcgat
    52321 aaggcttact gttggactta aaactccttt agtgacaaaa gagtcttctt gcggcaaaat
    52381 acagttcaga caggcggttt tttgagggac aattgttgtt gcttgcccgt aaaattcgct
    52441 tactccacca tgtattaaga tttttcctgt tttaaccgca attttgttca aaaggaattt
    52501 tgattgaaaa ctgtcaaagc agtcaataat taaattatac tcgcttacaa tatcattgta
    52561 attgttctca tcaagcctta tcggatatgt tttaacttca atttcagggt taaattcatt
    52621 aatccatttt tttgcacttt caactttgat tccacccaga ttattgaatt tgtgaatata
    52681 ttggcggttt aagtttgaaa gctcaaggaa atcattgtca acaattccga tttttccgat
    52741 gcctaagctt gcaaggtttg caattacggt tgagcccagt ccgccggctc cacatataag
    52801 aacttttgat tgaagaagtt tattttgacc ttctagtcct actttttcga ttattatgtt
    52861 gcggttgtag cgttgtaatt gaccggtttg taaattatac ccctcacccg aatttctaat
    52921 actcgtacct cgtattgaaa ttctaccctc tcccgcaagg ggcgagggta aatgcgaatt
    52981 atttgtatct tcagaatact tcgtcaataa tacctccgcc aagcagtaca tcgccgtcat
    53041 aaaaaactac cgattgacct ttggtaagcg atttttgcat ttcatcaaat tctactaaaa
    53101 tgttgttgcc ttgcggtatt aatttcacat caacaggaat ttgcgccgag cgtacttttg
    53161 cggttgcttt tgttttgttt tttagttcat tgattgatac ccaatttata tcaactgcta
    53221 aaagagattt tttaaatgtt ttgtcaattt ctcctactat aacctcattt ttttctttat
    53281 tgagttcaat tacatacaaa gggctcgtgt gagcaattcc gattcctttt ctttgcccta
    53341 ttgtaaaatt ccaaattcct tggtgtttgc ccaaaatttt gccgttttta tcgacgatat
    53401 tacctgtttt cggttttact tcaagtaatt cgttataatc cccatcgtaa aagtcctgac
    53461 tgtccggttt gtcgtggact tttagtccgt tattttttgc tatttttctt atttcgtctt
    53521 tttcatagca gcctaagggc ataattatgt ttgcaagctg gtcttgcctt aatttatata
    53581 agaaatagct ttgatctttt ttgggattta ttcctttttt aataatatat cttttatttt
    53641 ttttatcaaa ttccacttgc gcgtagtgtc ccgttgcaaa tttatcgaac tctaagccgg
    53701 cccgttttgc caaaaaaggc agaacgtcaa attttatcag gctgttgcat cttatacagg
    53761 gattgggggt tcttccggat agatattctt gtttaaaatt ttctaataca attttttcat
    53821 attcttttga acagtcaaaa gtgtaaaaag gaattccgat tttgtcagct attttttttg
    53881 cctcttcaat atcttctttt tcattggggc tataacaggc gtttttacag gaattatctg
    53941 aagaaatatc ccctttatat ttacccccgc tccaaatcga catggttaca ccgataactt
    54001 catgtccttg ctcttttaat aaaagtgccg taaccgatga atctacaccg cctgacatcc
    54061 cgactaatat tttagaattt ttattcacca ttcactattt accatttact atttactatt
    54121 tactatttac tatttatcct ccaccgaaaa atccgacaat ttctatactg tcattttctt
    54181 ttataggtgt tgtagaataa atttccttag gaacaatggt taaattttgc tccacaacaa
    54241 acatattacc gcttacttga cgttctttta taagctcttc tatcgtagaa gagtttttaa
    54301 gcacaatgtc tttgccgtta atttttaaat taatttctgt cattttgtcc tcaaaacaat
    54361 atataaacag ataatctcat agaaattatc tgttttcaag gacaaatttt ttaaaccaaa
    54421 ccttctctga tggcttttac tgcagcttga accctgtcat caacggacat cttttgcaat
    54481 attgagcaaa catgagcttt tgcggtgtga acactgacta ttaattcttt tgctatctca
    54541 gtattgcttt tgcccttgac gataagttcc aaaacctcaa tttctctttg ggttaatggt
    54601 ttattgtttt ggtcggtaaa atcaatttcc cagttattca tagcacaatt cctgaagttt
    54661 attccatact ttgttattct aaattaaaag ggagacaata aaatgaacat attttccgtt
    54721 tttagatatt gaatgaaggc ataattttta aaatataaat ttttctatcg gcacgtttat
    54781 gaaaaaactt tatatattgt atatattatt tatttaacat gttatctatc gattaaaaaa
    54841 tgtaaaactc ataaaggcta tatataaaga gattaagcct gtttttttgt gctttaaaaa
    54901 taagaggtat ttgtaaagaa atgtaacata ttttggttaa atgaggtgct ttaagcaatt
    54961 cattaaaagt tatatacttt atatatttac gagagtaaac aacacgagga aaaaaacatg
    55021 ggctttttat tatttgcagt aagaaaatta tttcttagaa gaaaaagtaa tgaccttagc
    55081 tttagatcca taatgctatc tcagaaaaag caagagatta ctgaacaaat aaattcggta
    55141 atgcagggta tttctcaggc aaaaagtgcc gctgctttca cagccggtca aatgcaaagc
    55201 atttttgcaa aacaacaaaa ttcagaaatg agttctattt ttgaagactt taatgctaac
    55261 agtaaaaagc atgagtttta tgaagcgttt aacggtctta cggatcagaa agaaaaaact
    55321 gcttggttaa aagaacatca aactgccttg actgatttag gatggagctc cagtgataat
    55381 aatctggatg cttgggaaaa ggttaaagct gaacttagcg aaacagataa tacaagaatg
    55441 actgcgttac aacaagcaca aatggaacaa aacactaaaa ttgcgatggc aacacaactt
    55501 acaaacaatg tttttgaatc aatgtctaac gcatcacttc cttatctgaa cgcagaggat
    55561 tcaagaataa gtttagaaat ggctaatatc gaatcacaat tgaaagctgt aaacggtgaa
    55621 ctcgaaggag ttgaaaaagc agaaacagag gcagcaaaaa gtgaagctcc taaattcggt
    55681 ttaggctaag ataaataaat taaacaaata ataaaaagag ccctttgcaa aagcaaaggg
    55741 ctcttttttg attaaaacat ttagtattca tctattttta ttgcattcac ggggcaggca
    55801 agtgctgcgt caatgcaatc atcttcgttg ccgtcaacaa attcggggtt tacggttgca
    55861 aaattattgc ggatttcaaa tacttcgggg ttaattgcag agcatgctcc gcagccggta
    55921 catctgtttg tgattgttac tagcatatac tttctcctaa atctctacat ttattatctt
    55981 cacacatttt gtgaaaataa aaattatctt aatcggcaat tcaatatagc cgaaatgctt
    56041 ataatttcct ctaaatatat ggttgatttt aatgaaaata accttaaaat acaaaaaaag
    56101 gagatttatt atgtcttata aaaatttaaa tgcaaattct ttgcttacag gaatttatgc
    56161 cggattaaca aatacttatt ctatacttgc gcaatcaaat cctaacggtg ttactttaga
    56221 cagtatttca aagtcttatt caaatacttc aaacggatat tcaataaatc cttcttttgc
    56281 ctcatatttg cagacaaatt tttccactct ggataaagat aaagacggaa ttttaagttc
    56341 atctgagttt agtaatttta caaatcagct ttctacgcag gggttaacgc aggcgcagtt
    56401 gactcaatta ggtaccgcaa ccggactttc atctgaagct ttatctgaag ttttatcaca
    56461 ctttgctcaa gttgatgcaa acaaagacgg gaaagttact tcggcagaaa tttcttctta
    56521 tcaggtaaca agtgcaaaag aaaagaaaat ggaagaattt acaaatcgtt ctgcgggtaa
    56581 tatgtcagta ttttacggcg atgacagtgc ttctgcagca gattcatcaa gtttattgga
    56641 ttacagatac atgtcagacg acgaaaccac ttagggcatg ttggcacaaa tattgcagaa
    56701 acgtcatgct gaacttgtct tctcttcttg tttccgaaca tagtgaggat tacaagggag
    56761 agtatcggaa cgatgtatca gcatcttata attgaacaat cttgagacct tgaaacgagt
    56821 tcagggtgac gtgttagttt tttgtgtcga atcgccctaa ttaaagatag aaaaccaaat
    56881 ttatcataaa atcggcaatc aacatgtaga ttgttgattt tattgcagct tgtgttgttg
    56941 aaattcctac atctttagca ccgccttgtg ttgtatagcc ttgcgtagcg caaacaagcg
    57001 cgattaaaat gccaaaaaca aaagctttaa aaaggctgat ataaacatct ctcatttgaa
    57061 gccaaagcca aacagaattt atatatctgt tggggtgaag atttatagtg aaaaaacaca
    57121 caaacattcc gcctacgatt ccgattaatt ctgcaatcaa aactaccatt ggaactgtaa
    57181 ttcccgcaga aattaatctc ggagtgaaat agtatcccac agggtcaact tttaaggttt
    57241 ttatggcatc aacttgagat gtaacctgca tatttgctat ttctgcgcta atagctgttc
    57301 ctgcacgcgc acctatggca agtgccgcaa atccgggggc aatttcgcgt aagataacta
    57361 tagtcaaagt cccgccgatg tatgtttcag cccctgacat taaaaattgt tttgatactt
    57421 gaatggctat tacggcggcg gcaataaaaa caattattat tgaaataggc agggaatcgt
    57481 agcttattgc agctgcttga gaaatcatgg ttgaaaagcg catttgtttt gaaaacaaat
    57541 actttatgct ggttattaaa ttttgaacac atagtccgaa aaaactaatc ataaataggc
    57601 atacaccatt ttttatattc ttttactttt ccgcatacaa catcaaaata gagtttctgc
    57661 agctcagaag aaattgttcc tacttttccg tctccgagtt gtctgttatc aatagaaacg
    57721 atagggctta cctgtgcacc tgttccgcag tagaaagctt cgtcggcaag gtaaagttcg
    57781 gtcctgtcga ttgttctttc ttctatttca agcttcataa cctcttttgc tatttgtata
    57841 acagtatttc tggttattcc ttgtaaaata ttatcagtag tgcttgttgt aacaagtttt
    57901 ttgccgaata ccaaaaacag attcattgca gagccttctg ttacttcacc ggtttctgaa
    57961 agtgctatag catcgtcaaa gcctgcaagt tttgagtcgc ttgaaataag tgaggcatta
    58021 gcataagcac cgccgatttt ggctcttggc ggaagtgcat tgtcgctgtt tctgcgccaa
    58081 tttgaaacac aaacttttaa gccttttgtt gtatctatgt aatcttccat agggacggtg
    58141 aaaataagca gtgaatcagg attattaagc aaagatgttc ctattctctg tgccgattta
    58201 taaagagtcg gacgaatata gatattttgc ctgtatccgt ttttgtttaa aagttttttt
    58261 gttatttcac aaagttcttg gacactattt tcgactttca tgtgcataat ttttgcactt
    58321 tgattaagcc tttcaaaatg ctcgggcact ctgaaggcat aaagctggtt ttctttttcg
    58381 ttgtaataag ctcttatacc ttcaaaaatt gatgttccgt acaaaaatcc gtgagtcata
    58441 acaggaatac atgctttttc tttttctacg tattgcccgt tcatataaac aaaatcattc
    58501 ataaatcttc tccttacctt atataaatat ctcataaatt ttttatattt gatatagaaa
    58561 tatttattat tattacattt tttaattttt tgtggaaatt atcttgactt tttaataaaa
    58621 ttttattgag gatgttatgg aattgatttt aaatttactt tatttttatg ttgctgtgta
    58681 ttcggtttac ttttttgtgg cgtcaattaa aaatctaagc gataaaaaat ttgccataca
    58741 gcaaaaatac tcaggtatta acgctcaaag cgagctttgt gtaattgttt attctcacaa
    58801 taatgaaatt acccttcaaa atcttgttaa acagctcaaa tctcaggatt atccgattga
    58861 caaattttct gtatatgtga ttttggataa ttgttctgat aattcagaaa aactttttat
    58921 aaatgaaagc tttataaaag tttttaatat tgcagaccaa gacactgtgg gaaaagatca
    58981 ggcaatttca attttacttg aaaaactttc aacagttcaa aattttaatg catacgtttt
    59041 tcttgatgca agtcgttata ttgatgaaca ttttttaagc agcgtaaacg catcattaac
    59101 cgatgagccg gttgtaagcg ggtcaactgt tttggttagt gaatatcctt catttaaaga
    59161 taaagttaag atttcttttc acaagtatta tactaatttt attcaaaaaa gccgttctat
    59221 attgggactt gcatcaataa tcgattcgga tattttgatt atgaggcagg aaattataga
    59281 aaaaatcggt tgtgttgatt ttcaaagtat taattcggaa cttaaatata gcctgctttt
    59341 atctaaagtc ggtttgcctt gttcgtttaa cccgaatata aaaacttata tcggtgttga
    59401 agattttaat ttgaggattc cttctatcag tgcaaggctg aatcttttta aaaattgttt
    59461 tacacagctt tggaccaaaa attttgtttt tatagaacat gttttttctc ttttgacacc
    59521 taatgtttta ttgttgactt ttatatattt gtacttgctt aaacattcct ttaaatatta
    59581 ttttatagtt gatttttctg ttgttttgct ttcatttata atgcttattt tgggcttttc
    59641 tctaagctta attaattcaa aactaaatag ggaagagatg ttatacctgt ttctttatcc
    59701 gttttactct ctgggacaca ttataaagaa tttaaaactt gtaaagcgga ttaaaagtaa
    59761 aataacaggc aaagatgaag tttttgtaaa cacagaaaag cttgttaccg atgttcttgt
    59821 gacagacggg aaaagcaata tct
//
LOCUS       DAOQAU010000007        58529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_397_length_58529_cov_7.123576, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000007 DAOQAU010000000
VERSION     DAOQAU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 58529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58529
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_58529_cov_7.123576"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>757)
                     /locus_tag="PKI94_02215"
     CDS             complement(<1..>757)
                     /locus_tag="PKI94_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25586.1"
                     /translation="SLIIRDTGAESKFTIEHGLKDLDTKALSLDTLIDDIYKFARAAK
                     SPKILETFKTSSLADNVFIKRLKALNTKTALLGLGMAVFVGICVQPFNMYLTKKKTGK
                     GGFVGVEGREPDKSLGFKILKSATAMAFALGTLATIGKSGSDIFRKLQFKGFTPTIEQ
                     FKLVYGLTIMSRLFSARDKNELREASIKDTLGFLNWLVFGGFVTKLAAAGFEKMSGQK
                     LINYNKVSNGKGWFNWITRATIKTRDEILHSDLK"
     assembly_gap    758..767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<768..1364)
                     /locus_tag="PKI94_02220"
     CDS             complement(<768..1364)
                     /locus_tag="PKI94_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25587.1"
                     /translation="MKVQIPQTQFNNQKQIGNAYRHRDVSFGGPVDGAFQVFWKFLDT
                     NKAWGANAVDIGAMVLPRTLVDFTRGPAAGAETMRREMSGTTNHTLIGTYGLVAASLL
                     GFGLNKAFPNVGVNKMFINSDNIDVMAELWHKHHNEGGQGIDGYLNELIDRIRGFNPD
                     KKKRVGWTSLEESKQEVAKTLKEELEKGKLSKESKQYIK"
     gene            complement(1387..2955)
                     /locus_tag="PKI94_02225"
     CDS             complement(1387..2955)
                     /locus_tag="PKI94_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25588.1"
                     /translation="MIEKINTNSINKKISTPSKNPQFGGLGDAILRGIQVCETNPMVN
                     VAVLDLSTDIIPRTARETKTNIFAGFEAFRRESSGLIVNCLLPSFIVLGIAKLFEKVT
                     MGNKTKMGSCWANEETINTVAHYWENAKGEGTSKVEDTIKTLLKDIHGKDGEVLKSFN
                     DDSLVEKSVKLLAQAVNEKNPKAVKKLIKEAFGHITNQTHISENIKIGSKDGKYFSQN
                     LSSVMRDIPKVLQELIENPQAKDLSWFKNSARRLVNTKSVLGLSIIIPLAISMQPLNR
                     WITSKISGKKGAPIYKDFKEAEHKELTAEEKMKLFSGKIASVGAMIGVALLAMLKKPS
                     LQMFQFSGLFPTMNQARLISASTFSSRIASSENEDELKESTVRDIGTFSGLYFLGDYA
                     SKITATIIEKTKGIKLLNYLSDKPDKSQGLIKKFLHWAKDTKLKSSHEVLEGAKNYRA
                     ICQLSNIGFSLLLLGIFIPFYTRGKTDKKHREDLKKAGVDNATIKKYYPNFMMNSANK
                     VSSKKIYKAFFTSN"
     gene            complement(3258..4802)
                     /locus_tag="PKI94_02230"
     CDS             complement(3258..4802)
                     /locus_tag="PKI94_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNW25589.1"
                     /translation="MSEKFLYNRLKNKQTNFNVWMAFPGIYSFALSSLGYMWLFKTID
                     EVEDVNVEMVCTDTKKTSLMIKDINLIGFSFSFDLDFLNIFKILQKYKIPFKAKDRNS
                     SHPLVFGGGPVLSANPEPFCEFFDFIIIGDGEQTNIKAIQVCKENKSLEKAKILEKLS
                     EIEGIYVPSLKQKFVKKSHCELSQCISTPILSEKSFFKNTFIIEIARGCSNRCGFCLA
                     SYLNLPARFIEYEEIINTIDLGLENTDKIALLGALISAHPRFTEICEYISIKASDKKI
                     EMSVSSLRADSIAPNVVKTLVKCGQKNTTIAIEAGSERLRKVINKNLSEEQILQTAKT
                     ARQNGLKGLKIYAMIGLPTETKEDLIETVNLAKKLKAENKGFDLTFSFNSFVPKPHTP
                     FQWCKRDDTKTLEKKINYLKKEFHKLGIKARFSSPKWDYWQTLLSRGSRDLTDYLVRV
                     YELGGNIGAFKSSYREFVKKKLLPDSDFYALREYNSEEILPWDFIQIPPGKDFLKKEY
                     IRLLKN"
     gene            complement(4799..5827)
                     /locus_tag="PKI94_02235"
     CDS             complement(4799..5827)
                     /locus_tag="PKI94_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNW25590.1"
                     /translation="MIFQEYMTTKNVIFFILILITLTFISQIKAIAMLFFASYIIACS
                     LNPLVDRLSKKIKRSLATSIVLFGTIIISFACFVPIVLVAIEQIKGFVNALPAQLKLL
                     EDFTMTHEFYGHKLSEFLNFESIVKSSSPFASGVVNQSINLTLGITQGIIFLLAIFMI
                     VFYLLADKDEIKKSFISIFPDKIKERADLISQNISHKVGGYVIAQILNMIAIGLITAV
                     ALFLIKVDYALLLGLITGVLDIIPIIGPTIALGLCLIMCYQMGPVGIALVIFAFLAAQ
                     WVSNNLVRPVVFGRFLDLHPLVIIFSLLVAAQFLGVWGVILAPAIAAIICVLFDEIYL
                     KTVNNGQE"
     gene            complement(5839..6078)
                     /locus_tag="PKI94_02240"
     CDS             complement(5839..6078)
                     /locus_tag="PKI94_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25591.1"
                     /translation="MKNLYDINEWSDIQIHQKIGEILMQSGKLSLVHLGMALDIQKFE
                     DIQLGEILLGMKIVTQTDINQALFVQEEIDKNIKQ"
     gene            6383..7402
                     /locus_tag="PKI94_02245"
     CDS             6383..7402
                     /locus_tag="PKI94_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25592.1"
                     /translation="MNVNSIKNYDFKNHTNISLNAPLKNGASKSKISFKGGEYNLANM
                     MGLIEGLGFFGAFFIIDSLSLITPRIVVGLNRDKDKLGHLNYKAGAEEAGREVLSGPS
                     MFLIPIGIFEVVRRTAAASKIPKNTMKVLFEHTKQTLKGLGDTSLLSNKKEFSEKFAQ
                     QLFDKAFGEFDLGDKAAQFKKRFTTRLVWCANSTSDKKSFKKNMAAFEKIISEINNSN
                     RSKTPIHPKIIDIGDNVKVRAKDLFEDFRHYSSDIITKISKRKWIDDASKSLKKEAVN
                     WTDNMKKIRLSLRFGACVASFFAVGAFLIHLPKLYQVSKDSSPAEESAKRASLEKSKG
                     GTNAS"
     gene            7392..8315
                     /locus_tag="PKI94_02250"
     CDS             7392..8315
                     /locus_tag="PKI94_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25593.1"
                     /translation="MQVRNISPEIINKNINKTSQNPSFGNILTKSRKAVLDLFEPGRA
                     GEMGMKLFVINAYVFLLSGRLLSSRDKKGEKTQKETAMDYIRNNNEKRETIIRDVPTI
                     LLAVMGVPWIEKFVSKRLQKSQGFAIHDIQKDKLGNIIPKYQVGDWYIFDKNLKTGFK
                     GFTQRLADMGGNLHKIYSHLDEGIKDKIKGFSLQNDEFMNQLFADKELTKEVETAFSN
                     PGNKAFRQAGFLKALPKVFGFVSTLLLIGILIPKFNIFITEMLHKGKKQEVAQENKDN
                     NQKPAEQKQTSTNLLEHAKNNSNTSNVKPNI"
     gene            complement(8367..9641)
                     /gene="metK"
                     /locus_tag="PKI94_02255"
     CDS             complement(8367..9641)
                     /gene="metK"
                     /locus_tag="PKI94_02255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNW25594.1"
                     /translation="MASKNFLFTSESVTEGHPDKVCDQISDAILDEFLTKDPKSRVAA
                     ETTVTTGMALVCGEITSDCYVDIPQVVRNTIKNIGYEGENCGGFDYKTCAVLTSIDEQ
                     SCDIAGGVNKAFETRDDNADTSNMETENLGAGDQGIMFGFACDETPELMPLPISLANK
                     LSFKLAQVRKQGLLKYLRPDGKSQVTVEYTEGKPSRINTVLLSTQHDPVINGEEDNHK
                     IQQIIKNDLIEYVINPVFENESLKPDSDTKYLINPSGRFVIGGPQGDAGLTGRKIIVD
                     TYGGYSRHGGGAFSGKDPTKVDRSAAYAARWVAKNIVASGLAQKCEIELAYAIGVAEP
                     VSIMVDTFGTGKICDDDICELISKNFDLRPAAIINQFDLRGLPAKNNGKFYQKLAAYG
                     HMGRMDFEVPWENTDKAQILKEQVNLNCFCTK"
     gene            9872..11575
                     /locus_tag="PKI94_02260"
     CDS             9872..11575
                     /locus_tag="PKI94_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNW25595.1"
                     /translation="MDSKEKRFRVFDQRLGCWNICFAIFAIILIIYLFCIQVIDIKQY
                     RIKAKKQRSSKNFIMRGDIFDRNGVKLASDKIYYDVYAHPADYDHSPEELAKILAPYL
                     KISTATLVEKLSKQDSVITLKKDLDRNAAQAIKKLGLREISLGKKNTRVYPQGSMAAH
                     VLGYYNFDADIASGIELTAKDQLEHVEKNVQTERTPDGNIIYSFSTDPVATSSPLKGK
                     SLTLTIDSAIQHICEQELHRMIAKRSALRGTVIVMNPRNGEILAYAVYPYYDPNNYKS
                     ATQRQIKNWTLTDVFPPGSTFKTITVTSAMALGKINEHTTVYDSGKIRVGWWEIKNYD
                     YSKRPCPGVINLEYLFEHSSNVGAVRIAQKMTSQEYYSMLTKFGIGSKTGIDLPGESS
                     GLLPKWQKWDPSTHASMAYGYAASLTPIQMVSAVSALANDGIRVTPHVIKYSPEEAET
                     KIFKTEVMPPETARTITKILTKSIENSKSPIKMDAYYIAAKTGTSRKPNENAAGYSNK
                     LYTSIIGYLPASNPQVLIYVLVDSAAGGEVWGSTIAAPVFKEIASQSARILNLKPDKS
                     K"
     gene            complement(11578..12249)
                     /locus_tag="PKI94_02265"
     CDS             complement(11578..12249)
                     /locus_tag="PKI94_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25596.1"
                     /translation="MRIQNTTFKPNQPAFKQIYKVTVAKKCFTDDANNSISPSLLNKE
                     QYIRELIKENMVEEIKKFVLRHGVYSLIEPVRHNRLQKVLESDFLPDFSVVGEQSLWS
                     YLEDAVENTFPMEYVINTINPNIKHPMNSKLNTFYVYSQEHCDIFQEILSEGDSYYDK
                     FLNSKEARESRCPQVWAYTKKMETIKKQLDEAFEGEKESEFIASSKESLRAILKMIVE
                     ENIKK"
     gene            12401..13861
                     /locus_tag="PKI94_02270"
     CDS             12401..13861
                     /locus_tag="PKI94_02270"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNW25597.1"
                     /translation="MKLSDLISNLQYEEKLNFEDVEIRGVSYNSKTTKQGDIFVCLVG
                     EHSDGHQYFKNAQENGSSAFLTEKELDTKLPQIIVDSTRQKIADIADKFYDSPSKKLN
                     LIGITGTNGKTTVTHLIQKIFEENKQQCALIGTLGYKLTSESEYKEAKHTTPQAPELQ
                     FTLDLIADKMKIPNCVMEVSSHALEQNRVGGCVFKGAVLTNLTQDHLDYHITMDNYFE
                     SKAILFKEVGEFAVINVDDLYAKRFLEVIPEGVKILTYGIENSADIMAKDIEFSPKGA
                     EFALEGGKFGAQNVCLQMNGMFSVYNVLAAITTGLASGFELKSCINPLEQTQGVAGRF
                     EVVAQNPLVIVDYAHTPDGLENVLKAAKEITPKGSELICLFGCGGDRDATKRPKMGAI
                     AEALSDKVVITSDNPRSEEPQQIITDILAGLRSVTSEKVFVEADRGRAIALLKTICKN
                     NDVIVIAGKGHEDYQILKDKTIHFDDREEARKIFNV"
     gene            13854..14819
                     /locus_tag="PKI94_02275"
     CDS             13854..14819
                     /locus_tag="PKI94_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25598.1"
                     /translation="MFEHFYKSKLLVEQHFHGAFGVDFSVCEADEILFLAKKLLQKGI
                     GGFYPTLVTDTVENIKRQIGRIKTASQKQSCEEAKILGIHLEGIFLNPEKSGIHDKNL
                     FLNPTVENYKLIEDDFIKIVTLAPELDKDFELSRYLKLKGVKVQAGHCVGSDLSYCDG
                     ATHLFNAMSGVNHRENSTALSALDDDRIYTEIIADGIHLSEDILKLVFKTKPHDKIIL
                     VSDSLPIAGSDLKEMAFGGKRIFYDGKKATSKEGTLAGSTAFLDEIVRHLSLENPENF
                     EKYVKMASDNLYKYHNIELDGCVFWDENFRIKAVEKDGVVLYKNN"
     gene            14877..16577
                     /gene="ilvD"
                     /locus_tag="PKI94_02280"
     CDS             14877..16577
                     /gene="ilvD"
                     /locus_tag="PKI94_02280"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNW25599.1"
                     /translation="MLKQVQHDRLKKGINKMKSDEIKKGISRAPHRGFLYATGVSKEG
                     LKRPFIGIASSFSDLVPGHIGMRDLERQIEKGIHSGGGQSFIFGTPAICDGLAMGHSG
                     MRYSLPSRDLIADCVESVAAAHQLDGIVLLSNCDKITPGMILGALRLNIPAIVVTAGP
                     MLDGCSKGEKLTMIRGFEAIGKCANGEISQERLFEIEENACPSAGSCQGLYTANTMAC
                     LTEVMGMSLPYCATASAVSSQKRRIAFESGMAIVDMVKSSVKPLDIITKDSIRNAIVM
                     DLALGGSTNSVLHLLAIANQAGVNIFLDDFEKLSHVVNQIIKLDPSSTTTMSDFHIAG
                     GIPAALKTLWGKTPELKNTKNCVGLSVEKIAKAAWVDENVIKPFNAPLTKNAGLGILY
                     GNLATEGAVIKISGVDESCFEFEGTAMPYDSEEEAMKALENNQIKAGHVIVIRYEGPK
                     GGPGMREMLAPTSLLVGKGLGKTAALITDGRFSGATRGICVGHISPEAALGGPLALVK
                     KGDKIKIDINKRSIELLVDEAELLKRKAELKPFELPQKGYLARYAQAVKSASVGAITG
                     "
     gene            16639..17892
                     /locus_tag="PKI94_02285"
     CDS             16639..17892
                     /locus_tag="PKI94_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNW25600.1"
                     /translation="MKKLIDYKEEIQKCSKCGLCQSVCPVYKVTGNDCAVSRGKFIML
                     GGIIKGDLKLKKNVNKYLDMCLKCNACKDFCPSGIDAREIFTCAKCEYFEGANSKFVK
                     ILNSPPVFNLFLNSAKICTNTYRFLKFDRILKLFYPILLKSNFGKRAVLANEFIAKNS
                     NILTKCTYSPIHLFTHSPKIIYFKGCVNEYLNPSVKNAAENVLKKMGVNVLDIKFQCC
                     GLPFLSVGNAEQFKKQAEFNLAQIPDDFANEFDYFLTDCASCQDAFREYKKVIDDEKL
                     MQKLDKILEKSLNINEFIVKNTQKFVFDKKIKITFHKPCHLENIDFLQEFLSKAENVE
                     YIEMKEFDSCCGFAGAFALNNDKISKEISSKKAKNAIETEADYILTSCPSCVLGLKQG
                     LIENGKLIPVLNFVEFIQVFIKSLH"
     gene            17964..19286
                     /gene="murA"
                     /locus_tag="PKI94_02290"
     CDS             17964..19286
                     /gene="murA"
                     /locus_tag="PKI94_02290"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNW25601.1"
                     /translation="MSEKFRIKGGVPLKGEVSIGGAKNAVLKLMAAAILAKGETKIYN
                     VPDLTDVEIMSSVIEGLGAKIVYDKTEKSISIDATDLSSITAKYELVSKMRASFIILG
                     ALVSRCKEAMVALPGGCAIGERRVDFHIKGLEALGTKIKIENGYVHAKVSKLTGADVY
                     LDIPSVGATENLMLASVFAEGSTRIQNAAQEPEIIDLANFLNTMGADISGAGTSEIVI
                     NGVKENSLRPIEYTTIPDRIEAGTYMSAIIATKGKGIIRNVFPAHLTFFNDKLLKMGA
                     NIKLIEPTALEVSCKNRLSSINFVTQPYPGFPTDLQSMAMVLLSTAEGVGVITESLYE
                     NRFMQVPDLVRMGADIHQDRNHAIIKGVKKLAGTDVSASDLRAGASLAVAGLMAEGIT
                     TIENLHHIDRGYERFEEKIRLLGGKIERYNDENPPAKLKEGLYNETHL"
     gene            19270..19602
                     /locus_tag="PKI94_02295"
     CDS             19270..19602
                     /locus_tag="PKI94_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25602.1"
                     /translation="MKPIYKRWYDHDPLLLEVIELLRNYQEELKAQAEVFLKKIEEKV
                     SKDAIDRFYMLVKPVNGNRWYDHDPVISKTVELLRVVPPDVQKSCAKHFIDSLKEIGI
                     EYASPNLK"
     gene            19602..23240
                     /gene="mfd"
                     /locus_tag="PKI94_02300"
     CDS             19602..23240
                     /gene="mfd"
                     /locus_tag="PKI94_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNW25603.1"
                     /translation="MLDFDFDNKNFSRMKRNLAASKSFAITGLTSFLRLLLVQKIKEF
                     SDKKVLFITASEQTALKCQNDLKNAFEIDAEIFPFQSTSMYEPVSANLYDYAAQIELL
                     NSDKDLIVPVKALLEKFPDKKFFEKNSIYLKIGDEIDTKKLAQNLINLGYKRSTMASD
                     MGEFSIRGDIIDVFSLSQNPFRIELWGDEIVDLRIFDNQTQKSIEKVKEITIKPVYKF
                     IFDKSINKRHSELVSESHPLSETKIKLEEGYFEGIEVYQNYFNPDLVSIFDYFNPSPL
                     PSPARGEGEQLSSHPSPFTSHLIVFDEMSEIFSKYEFIAQNYQNQLGENIKTGLIFYP
                     SPLPPLPQGERGKTECPKNSTLRPLMEEINVSDIPPTPRPLREREDFQCEERALENRV
                     RGNLKNHFELEEFQNQTAGFVKIGFNNFIDYDCGDLTEFDSQPLKGYFSGIDEITDFI
                     SSKKGYKILVATDFPERVKEIFAQKNLFDFETAKSLSCSGCIIEDLKLILLTDKELFN
                     KRPKEITASKRAYYKEKPEFIESINDIKEGEFVVHSIHGVGVYKGISQQQLDGQYKDY
                     LTIEYALGDKLHMPAEQINLLCRYRGSGTLKPKLSRMGGSDWENTKTRVKKEVEKIAY
                     DLLRLYAKRKIQEGIVFEQDNPWQFEMEEAFEYTETPDQMKSILEVKSDMENEIPMDR
                     LICGDVGFGKTEVAIRAIFKAVTSGYQAAVIVPTTILALQHFQTIQERFKPFGIKVEL
                     LSRFKSAKEQKQILKELTLGSIDVVIATHRLLQGDVHFKNLGLLVIDEEHRFGVRHKE
                     KLKSIKENIDIISMSATPIPRTLYMSLSGIKDMSVINTPPKNRLPIKTFVGEFNESQV
                     KNAIVHELDREGQVFYLYNRVETIEEFKLQLQKIVPEAKIQIGHAQMDEKQLEKVMVD
                     FANKEFDILLCTTIIESGLDIPNANTIIIHDCDKFGLAQLYQLRGRVGRSERQAYCWC
                     FYKKSKELTQEAIKRLKAIKEFTTLGSGYQIALRDIEIRGIGNILGTKQHGHMVNVGF
                     DTYCQLLEETINELKGESTEKIPPAIVDINVTAYIPDEWVGSKEQKMIEYKRLSDVKS
                     QIELDYITQEWQDRFSKAPESVQNLIKLIKIRLAATDAKISSIRETHENIRIYTPYLL
                     PEWNIIKGHLPPNITKYIKFTSVPKSCTEGNSIILLDNSCMNFDEVFNILIDLFYYIY
                     KVSKGYTS"
     gene            23253..24413
                     /locus_tag="PKI94_02305"
     CDS             23253..24413
                     /locus_tag="PKI94_02305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW25604.1"
                     /translation="MNTKKLFATIAISALLLTGCSFGKKDVIIKVNDREITQTQYDQA
                     FDKAAGNSMFAKMGIDIKKDKNGFLYMMIKDRVINELVVKALIDGEIEKRNIKVTKED
                     TDKELQNIIDKIGSKEKFNEILKQNGISSSQFKKDLTEEVKMKKLVNSLAVIKISDAD
                     AKKFYRQNLNKFKYPDKVRASHILIAANPEEIKEKITSDKANKNLSEEEVKAKIKQEI
                     NSQHEEAMKILTEVKKDPKSFERIAREKSQDTTSAKQGGDLGFFAKQEMVEPFAKAAF
                     SLRPNTVSEIVQTPYGYHIILVTDRMSAGQEPFEKVKDEIKTYLEAQEQVKVLENLIE
                     SLKKQAKIEYVNPEYNPLTVQDILKKQAKSNPAAKEEMEPQSAAPPATPAKE"
     gene            complement(24473..24817)
                     /gene="rnpB"
                     /locus_tag="PKI94_02310"
     ncRNA           complement(24473..24817)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI94_02310"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            25557..25628
                     /locus_tag="PKI94_02315"
     tRNA            25557..25628
                     /locus_tag="PKI94_02315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25589..25591,aa:Gly,seq:tcc)
     gene            25653..26201
                     /locus_tag="PKI94_02320"
     CDS             25653..26201
                     /locus_tag="PKI94_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNW25605.1"
                     /translation="MISQFFSKHFNEILELSIVFITVLVLFKAIDIFNANAKKRILAK
                     RQDSTILGFMPLINKVLKFTILFLALATILQSHGYSITSLIAGFGITGLAVGFGAQST
                     ISNVFGSFSLLADKAYKIGDYIVINQTVHDKAVEGIVEDINLRSTKIRTTDGLIIVPN
                     NIVANGVVKNTSIKEKVIPNEN"
     gene            26191..26661
                     /gene="rlmH"
                     /locus_tag="PKI94_02325"
     CDS             26191..26661
                     /gene="rlmH"
                     /locus_tag="PKI94_02325"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: protein motif:HMM:NF000985.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNW25606.1"
                     /translation="MKIKIVALGKIKEKFLKEGIDEFLKRLTPYASVEIIELSPLEIK
                     DENLIQKTLKQEGEKILAQIKPDSYVITLEIDGANFSSEKFARKLEEIFNMGISEIVF
                     IIGSSHGLCPEVSTRANLKLSLSKMTFLHQFARLILIEQIYRAFKIQKDETYHK"
     gene            complement(26672..27178)
                     /gene="fliN"
                     /locus_tag="PKI94_02330"
     CDS             complement(26672..27178)
                     /gene="fliN"
                     /locus_tag="PKI94_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNW25607.1"
                     /translation="MPDEEKIEQEDIKQEAPAPEVKEEKEEVVAQPTEEVIVKREPAE
                     STVVDNEDSQIQATDEPVTVRPIKFASFEDINPSQGEVNKNLDILMDIKLQLTVELGR
                     TELPIRKVLELTRGSIIELEKVAGEPVELYANGKLVAHGEVVVIEDNFGLRITSITEP
                     EDRIKGIQ"
     gene            complement(27193..28290)
                     /gene="fliM"
                     /locus_tag="PKI94_02335"
     CDS             complement(27193..28290)
                     /gene="fliM"
                     /locus_tag="PKI94_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNW25608.1"
                     /translation="MPETPEEKEQSAETEAKSEEKSAAGLEADSAVAVADENTTNSES
                     SAEDIYNPYSGDNADEDYKKGYKLYNFRRPDKFSKDHLRALQDVHREFSRQISLVLTA
                     YLRMHIEIDVVSTDQLTYDEFMRSMPSPITIGIFEMNPLPGQILLGISFEVLSCIVDR
                     MLGGVGLSENKQRELTDIEEALAKKVIERTIKTLESAWSNIVPVQSELMGLDNSYQMI
                     QVATSGEIVALITFEVQISGKYFGLISLCFPYPVLETVLGHLSTQHIFQTKGIVATTE
                     ERQKMIQKLNTSKVDLSVVYGSCQISLEDFLDIKEGDILKMDNKTNEDLVVKVNDEKK
                     FFARPGTLKNHVCVKIMDRYDPTIDSLKGYI"
     gene            complement(28300..28953)
                     /locus_tag="PKI94_02340"
     CDS             complement(28300..28953)
                     /locus_tag="PKI94_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNW25609.1"
                     /translation="MAKPNQPKDIKPKAEGEEGASEASPKGGGIKIDENMKMIIINLA
                     TTVLICILFLSVNYILQANLLNSKLAQKTSATEETAAEGEEGAGDEVQRGIIVDLGDF
                     ILNLSDVSPRKYLKVNVALEVSKSPQDMELEAESAKGGGHGEGAAAVNPVEAEMAQFK
                     PSMRDAIITTLSSKTSAELSTVAGKELAKEQIAEAVNGIFSGEREVIRVSFGQFIIQ"
     gene            complement(28969..29649)
                     /locus_tag="PKI94_02345"
     CDS             complement(28969..29649)
                     /locus_tag="PKI94_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNW25610.1"
                     /translation="MRNDYYTNSKDYINRWVVSYADFVTMLLALFMVMFAISQLDNQK
                     LMELQAKLQKTFSDTPQIENVTKYHKITEGDKSYSTQSLKMLFEQNLSQTKSIKLIKA
                     DRGLIVRVSDKMLFDEGSADIKPRSKKALGEIIKVLTKIDNPVIIEGHTDSVPIKNAK
                     FPSNWELSTARATNIISYLTKTSKIPPKRLCAVGYGEYYPIADNTSISGRMLNRRVDI
                     IVLGKDSN"
     gene            complement(29646..30416)
                     /locus_tag="PKI94_02350"
     CDS             complement(29646..30416)
                     /locus_tag="PKI94_02350"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNW25611.1"
                     /translation="MNLTAVFALALGFVMVFITQIFEGGSISVLFQPTAFCIVVGGTF
                     CASLLNFNMQTLKTAFRHAKEIFYHQENKTMKAVDDIIQLAYFARKNGLLSLQGVLDK
                     VQDPFLSRGIQLAIDLNNPQLLYDLLSSEISYEEEEELINSRVFEALGGYAPTFGIVG
                     AVMGLIQVMSNIKDLHILGTGIATAFVATLYGVGFANLILLPIAGNLKQKTREKILLK
                     EVILQGIISIQMQENPAIIEEKLVAYLRFHNKKHMALR"
     gene            complement(30859..32763)
                     /locus_tag="PKI94_02355"
     CDS             complement(30859..32763)
                     /locus_tag="PKI94_02355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNW25612.1"
                     /translation="MGFLDKIKEFTGQISEVENRIYANKQDFLEIYERNLQLEKEISE
                     RTKELDIANQRMLTLQHIWEMMNSSTPLSNVLATITKSIKGDLGYLYSGIMQKKTDEK
                     GNYIKTITYAEDELIERLNLAFGVPIQTIKFDYCEDSLFAEALTNRRIVQTTDLRSII
                     KAVSKDLSEDSVGKILNNAKSRSIILVPLWTRNTEFGWLIVFSSREEASEAELDFLNL
                     FSKQIELAITIADLFQAVKNQAVTDALTGLYNRRHFEEFLKKEATRAERMNQPFTIIS
                     LDLDYLKQINDKYGHSYGDMAIKEVAEVLKKNARSIDVAARMGGEEFNIILPGVDSKG
                     GLIAAERIRKAISDCKIRKVGKITASVGLATFLEHSDKIDELLEITDQAMYMAKRNGR
                     NRVQVAKPISETSWQEVAITTFMDILSRKRLPIPKDVASELHEKLATSLEKSSTPKEM
                     LYSVADMLSATYNPIHAKGKTKSKILMVSNLAKSFDFPKEEIDKLRIATLLYDIGNIM
                     LPQEILQKIEPLTEEEQAEIKKHPLIAAREILKPISDIQDVIPIIEHHHENWDGTGYP
                     GKFSGEKIPLSSQIILITDAYFALIEPRAYRQAMSPQDALEVIKKDSDKKWNQDLVKE
                     FVTFIENELK"
     gene            32980..33687
                     /locus_tag="PKI94_02360"
     CDS             32980..33687
                     /locus_tag="PKI94_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25613.1"
                     /translation="MLRISSTMPIYTANVTSKNCIKSVTFKGKQVPEEIVIRPGQEIP
                     EIIKAKKVIIKGEYHHWIVAKDTKIICETCELEHAMFKEINASKSATLSKESRVNVLN
                     TNDWASISLSSVGKINAKGRVCMQWMASADEVNAGEILMINSRVKSLTTEKTADLSDS
                     KVKILTSPAVKMSNQSEVENLSADYLHAEDSTVKSGVVKKSIYKCNSAINVKCNDIYD
                     DTFSKSLRELQASECNR"
     gene            complement(33699..34763)
                     /locus_tag="PKI94_02365"
     CDS             complement(33699..34763)
                     /locus_tag="PKI94_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNW25614.1"
                     /translation="MSDNQKKVLIIRLTSLGDVIFTLPLACTLKDENYTVGWVVAEKG
                     LGVIKNNPKIDKVHFVPLKEWKKGPFSFKTFMEFFQIIKQIRKEKYDIALDCQQMFKS
                     LFLLLFCGAKRRVTFKDARELSFLGANEFVEPKAKFSDLNYHIVERNLDFARHLGIEP
                     KGINFALPSTSSDVKNKVSELLAQIDHSKPIVVISPATTWKNKHWDEKNWANLIDEIQ
                     GKCNIVFTGTDFNEDLIQRILGKTKTTPQYLNLVGKTNVEQLIELFSRSKIVVSPDSG
                     SAHLAWATQIPAVIAIFTCTPPKRFGPYGQEGKYFSVLGNKLNCQPCFKKRCRHKAGK
                     NECTKYPSSEEIISVVNKLL"
     gene            34931..36313
                     /locus_tag="PKI94_02370"
     CDS             34931..36313
                     /locus_tag="PKI94_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25615.1"
                     /translation="MAYNTITPLRQKLYTVHNNNFVKPKEDEESSHLPQPAEEQTEAE
                     QSPKLTLDEQKRVFPEQAEKNQASINSLKNAVNIAQILKDFRSTALAIGTPPDLNQEV
                     ETYLLLVDTQVRKENPNIKTIKSNLKNASALLDGYITETLQRPSNVVENWLDALFLQQ
                     VDFKFNENDVNPQFLVQFPDSVKTSQTENTQQTEETSQTEEKTNQSVIIPQDAQLKSL
                     FIQAKKLAYAGEAKRALETFEQARIRAIEVKDSETESKVLYEIGQIYDSKDFLAQALT
                     SYHKSLGAATDLNIKTKAHFSMAKIYDDVAQFEPAINHYMSSISFAGQNDNFAAQSTS
                     LTKIGNIFTDKYDKKAFEFYDEAKTIADESKTPKIKGYVSGNTAGAYAKFNNPKLALQ
                     NYSEAVQEYQNAKSPEQAAINYKKAAQLMIKLQNFNKAKSLLQKALANAKQTNNTELI
                     SKINSQLQTL"
     gene            complement(36366..36635)
                     /gene="rpsO"
                     /locus_tag="PKI94_02375"
     CDS             complement(36366..36635)
                     /gene="rpsO"
                     /locus_tag="PKI94_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNW25616.1"
                     /translation="MSINLKNKQEVVKKYGKNEKDTGSASVQIAMMSQKISELTEHLK
                     INKKDFATKRGLLMMVGRRKRLLSYLKGENLEEYRKLVKKLNIRG"
     gene            complement(36756..37148)
                     /locus_tag="PKI94_02380"
     CDS             complement(36756..37148)
                     /locus_tag="PKI94_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25617.1"
                     /translation="MKFVFIYIRDINEDFGMDFVTNDLKQRTLESINNQAEDARQKMY
                     EKRTIYEASMKKYGIFSELKEKAFTIFQSYKNTSKEEEFKTKYQNAQKSLFEAESDSD
                     ILRDSLRSSISFCSKMNESAFIANSILG"
     gene            37200..38489
                     /gene="purB"
                     /locus_tag="PKI94_02385"
     CDS             37200..38489
                     /gene="purB"
                     /locus_tag="PKI94_02385"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNW25618.1"
                     /translation="MIERYSLKEMKKIWDLQSKFGYYLQVELAVCEAYSKMGQISAKD
                     LDKIKKLAKFDLKRIDEIEKEVNHDVIAFITNINESLGNLSKYFHLGLTSSDVIDTAL
                     ALQIKDAAKIINKDLDNVISSLKKLIKKCKKTVCIGRSHGVHAEIMTFGVKLCSWLDA
                     FERVKENFNFALEKIMVGQISGPVGTYSNISPEIEKITCKILGLKPAKISTQIIARDI
                     HAQFMQSLALIASVIERFATEIRHLQRTEVLEVEEGFKKGQKGSSAMPHKKNPITSEN
                     LCGLARVVRSNSIAALENIALWHERDISHSSAERIIFPDSTILVDFMLERFKNILDNL
                     VIHEENMLKNTNLFGGIIYSQKVMLELVNRGLSREKAYEIVQKNALDAFEKSGNFKSN
                     LLKNKEIKKYLSQKEIEACFNAQDYLKNTDEICSKFD"
     gene            complement(38490..39071)
                     /locus_tag="PKI94_02390"
     CDS             complement(38490..39071)
                     /locus_tag="PKI94_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNW25619.1"
                     /translation="MEKTQNFIENRPQELCNMCGKCCRVATSSLSFQELKSLVRQGDE
                     YAKDFLNIFEPYQSIQDAKNVCQKTVENILSKLNSEKEDFDEKEATFYRCRYIKDDNL
                     CGIYEKRPELCKRFPSNPWVVVPPGCGYEGWLFKKREEKKQYIRKLKEDILSYEIILK
                     GDLPESERENILKILEKINDIIEIYSKYGAKDW"
     gene            39191..39922
                     /locus_tag="PKI94_02395"
     CDS             39191..39922
                     /locus_tag="PKI94_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNW25620.1"
                     /translation="MQNTVVNPQTVAPAQAVVTPAAGNQPQVATQPVVNPGVVATQNP
                     QTVQVPNYSGVNIQIYNPSVMTPGTNMAYPSNYYTTNYGPQGQPQQAAAPAAPAEGDK
                     KKTEKRDIVELTDDYIKNLENYLNSQDPEVRMMGAKEVLARLQEDDSRKDDKALNALV
                     NKMLQDPSQKVRVIALAALDSKAVTGDDLTVQILKNMQNKKTSDGQDAMQASQILLKM
                     SGNTVKKEFEVKDKPKKEEKSDKKE"
     gene            39935..40366
                     /locus_tag="PKI94_02400"
     CDS             39935..40366
                     /locus_tag="PKI94_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25621.1"
                     /translation="MNISNIAIKAIGIAGLGAVLYDSHTIGKEECNMNQRNLKTDSVE
                     KTFMDTISLDTPSKTKDIMKKQFLKYKLNENFTTFFTGAAGYVKGFTKMLVNNVIPFG
                     LALGTVATKGLASKACGVGLIGYGLVYLLHDVMGIFKPKKL"
     gene            complement(40456..40824)
                     /locus_tag="PKI94_02405"
     CDS             complement(40456..40824)
                     /locus_tag="PKI94_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25622.1"
                     /translation="MSIDRIGLNSFAVGAKMGGAGKAPEKKVEKEKESEGSAKRQEVS
                     PEAVLGFMGAKAVLGIESSSSLKKAFAMAESASPEVVARMTTMMDKFADEVGKNMAAV
                     QKELGLSEPAAREVALKMTE"
     gene            41024..41620
                     /locus_tag="PKI94_02410"
     CDS             41024..41620
                     /locus_tag="PKI94_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25623.1"
                     /translation="MPIKKINSVNTDAPSFGALPKPFVIKRAKTIDILEKMGKFSSPQ
                     QRLFLGVTALMTQPFIDLNNKSVDEKTRVVSCARTIAKNFIGTIVGITVRELCIKAID
                     FCTEVDAKKLKDTKYFKFKTALLPSNVKFKEVTRELVKHRQALGTFIALGAMLFTNFA
                     IDLPLTKWMTNLLVKKIEKGAQKKLEKQKQDKLQGGGK"
     gene            41667..42416
                     /locus_tag="PKI94_02415"
     CDS             41667..42416
                     /locus_tag="PKI94_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25624.1"
                     /translation="MKLSLQKTVDGVYKHFSHDLGFMLLVTGAVGWALSSLAHVTAVV
                     INKDIPKEQKNFLIPQEIADAFVNIAAFIAITHQFNKFGTRMVESGKLTTAPIRNFLI
                     KNQESFKNLNERIIDSNDLISKYFKKVFKNDSAAKQKLEPFFTKRKVNITDSDVFKNN
                     QDNIQKDFYSFYDGVDFISSTVGAIVSCNIVTPILRNIFGAKRQKSLIMHDKLHQSSI
                     MFAKPRVTLESYAQNVSQKTSLTSPNSSMKI"
     gene            complement(42413..42631)
                     /locus_tag="PKI94_02420"
     CDS             complement(42413..42631)
                     /locus_tag="PKI94_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNW25625.1"
                     /translation="MEENQNFSKNLTKREFEVLNLVTLGKSNTEIAKELAISSHTIKA
                     HVCSILHKMSVEDRVQAAVKAVREGLVD"
     gene            complement(42741..42986)
                     /locus_tag="PKI94_02425"
     CDS             complement(42741..42986)
                     /locus_tag="PKI94_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25626.1"
                     /translation="MKKTKKIIIKKAAQALVEYGLILALVAIVAIAVLTKLGGTITEA
                     GNKAGNTVSGVSDNASNNYCQSLTPPMTYNPTTGSCE"
     gene            complement(43016..43240)
                     /locus_tag="PKI94_02430"
     CDS             complement(43016..43240)
                     /locus_tag="PKI94_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNW25627.1"
                     /translation="MKKTAQSLIEYGLILALVAIVALAVLSKFGGTIGNVGNSTDTQV
                     QKVTENSGAAYCNSVSGQWNEDTKFCDITP"
     gene            43451..44188
                     /locus_tag="PKI94_02435"
     CDS             43451..44188
                     /locus_tag="PKI94_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNW25628.1"
                     /translation="MTMIKLENVRKSYRTTRILNNVSFCAHRGEIVAIIGPSGCGKTT
                     LLKCIDGLEKIDNGTITIEGHDITKKTKDLSKLRQNVGIVFQQYNLFPHMTVKQNIVL
                     APLIVKRMKKNDAEILAMLLLEKVGLLDKMDHYPHQLSGGQAQRVAIARTLAMKPKVV
                     MFDEPTSALDPHMKVEVLEVIRKLKNQDMIILLVTHELKFAKEIADRVVFLYNGQVIE
                     NDSAQNVFNSPKNEVTKKYFEKISGAE"
     gene            44200..44982
                     /locus_tag="PKI94_02440"
     CDS             44200..44982
                     /locus_tag="PKI94_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNW25629.1"
                     /translation="MKRQVIYIFLLGLVIFFSGCKKQDETSDVLDKVLSRGKLIVGVK
                     FDTKPFGYIDEKQNLTGFDVDLAKHIAKNLLGSEDKIEFKQVTPSNRILTLNSGQVDM
                     IIATMTITDERKEVIDFSKPYYMAGQAILVPAKSDIGSMADLNGKRVIIIFGSTAEKN
                     TRLMAPEAKISGYKTYTNGYSALKQGRADAITSDDTILMGFAMQDDSVKLLPKRYSKE
                     PYAIGFKKGESSTRLRQRVDLILDEMIKSGQLNKLKAKWIKY"
     gene            45041..45715
                     /locus_tag="PKI94_02445"
     CDS             45041..45715
                     /locus_tag="PKI94_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNW25630.1"
                     /translation="MYEMDFSTLLKNYPMIIEGILTTISVSLLAGILAFVLGSFFAYF
                     RTSPLNIISKITSFIVLFIRNMPLLILIYLFYKGLPSIHLVLSAYACGILALGLYTGC
                     YISDAIISGINAVPKEHFQASQSLGMSRIKSFIYIIYPQATRYVIPILGNQFMSLVKN
                     SSLVSFIAVTDIFYVVYKGISDTYRIYEYFILAVVLYLFLTGFVLLLTKILQKIYRIP
                     SQEVIV"
     gene            45712..46380
                     /locus_tag="PKI94_02450"
     CDS             45712..46380
                     /locus_tag="PKI94_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNW25631.1"
                     /translation="MTSDVIINNLPFLMKGLLVTLKVSFICIILSLFSGIILGTLRFA
                     KIPLISFIAGLFIDLTRAIPLILFIIFVHFSVSPYLFGYSAFLNYMGIDSLEMYSAII
                     ALVIFNCAYIAEIIRSGLESVGKDQILAAQSLALSKVQVIEHIVLPQALRRIKPSLVA
                     QLVTLVKDTSLASAIDLIELTRACEIVYETSYHEFEVLCFAACVYVSVNFLIQKMFNT
                     DIKC"
     gene            46489..47049
                     /locus_tag="PKI94_02455"
     CDS             46489..47049
                     /locus_tag="PKI94_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNW25632.1"
                     /translation="MKKRLGFTLAEVLITLGIIGVVAAMTIPTLMNQTGQAEFKTGLK
                     KMISGINQAVTMNVALDSVSFSEGDETNNLYKLFSDRMNVISSTSAAVGTGDINVDEG
                     NYIIFLNDGAAISWTDTVNETACTTTVGTNEATGVCNVLIDVNGKKRPNALTTSSTDI
                     KDQFGVRIGGQQALPGGVAKAVMYNR"
     gene            47118..47792
                     /locus_tag="PKI94_02460"
     CDS             47118..47792
                     /locus_tag="PKI94_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW25633.1"
                     /translation="MKISGEKQLDLLTNLVNFIFSIQRHLTFIKEVNNPELKPCIYAL
                     WHGHQCCIFGVEDKAHLNVLISRSIDGEIIARATEKLGFKTVRGSKGKKGSVEATMQL
                     LDRLKNSECIAMTVDGPSGPAFQVKEGVIKIAKMSGAPIIPVYWYSPQINFIKFPSWD
                     KLLYPLGATWLVNLYGEPIYVNSDNTDEQDEEARLKLQNALKELEEKAPQVHKEIYKF
                     RLWKKN"
     gene            47777..48658
                     /locus_tag="PKI94_02465"
     CDS             47777..48658
                     /locus_tag="PKI94_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNW25634.1"
                     /translation="MEKKLKIAAIQMSSKIGDIDFNIKKIDGLIKKNIKEEIDLLILP
                     EVWTVGWMPAGFKNSAKDIEKSKAFLFLSKIAKKYNTNIIGGSFITKRDSKYYNSCPV
                     INNKGELIATYDKMHLFSYYGCDEGAFVEKGNNPVMVEFCLNSKKIASVNDNCKSRQS
                     TSRNDIVKIGLSICYDIRFPEIYRAYANAGADLLINMAAWPKSRAGHWETLTKARAVE
                     NQCFMVALTQSGKITEDEWNLGHSRIIDFNGEVISQIKKGEGVIFAQPDFEKMYQFRK
                     KCTVLLDIQENYDVKLF"
     gene            48666..50030
                     /gene="dacB"
                     /locus_tag="PKI94_02470"
     CDS             48666..50030
                     /gene="dacB"
                     /locus_tag="PKI94_02470"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNW25635.1"
                     /translation="MKKIKLISTFIALFVFAQISIAKPLINPIFSAIWESDLSRRALI
                     GVSIKEVSNGKTAYEMNAKMPMSPASIQKVITLLSSIDTLKENYEFKTELYRAQDGSI
                     YLKLGADPYLTTRDLRELISMLPQKQDLNKPFYIDDSVMDSQEWGEGWQWDDDLNPLM
                     PKFGAYNLDKNLLKINFCPGSQQGAPAEISTEVFYPTAFSNNVTTGSSTEIKLDRKNY
                     ISPDVINAQGTISGDYSIEIPINHPRRYFVLRLEEVLRKQKVGYYGEFERMKLPKDAV
                     LLGKISHPISQAQSDILKQSNNMAAETVFKLAGGKYINNAGSAEAGVEMLNNYYKKLG
                     INTDSIKIVDGSGVSKNNLITADFMTNVLVKIAKEPSYNDFINSLAKSGEGTLTDRML
                     YFKDNLKAKTGTLSNISAITGYLTAKSGKTYAFCIMVNDPKSTNFDKKTFEEYVLRKI
                     YEEL"
     gene            complement(50015..51022)
                     /gene="ispE"
                     /locus_tag="PKI94_02475"
     CDS             complement(50015..51022)
                     /gene="ispE"
                     /locus_tag="PKI94_02475"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNW25636.1"
                     /translation="MREIKMKNDPSSGFQPPSPARGEGKLNPPRPLREREKFRNEVRV
                     SEIWVRGKKEIKVKCPAKINLTLEVLNKRQDGFHNIQSVMQTIDLFDFLTIKIEENCK
                     FEIKLSGTSDQIPYDESNLVYKAILLFVEHTNLHPHKISVFIEKNIPVSAGLAGGSTD
                     AAGTLWGLNKIFSDVLSKEELHKLCAKLGSDLNFCLEGGCQLATGRGEILERLPFQEF
                     CLALIKPKNLGISAKEAYTKFSQLKVKPNLNMSAKLIAALKSGENIVPFLHNDLETAL
                     IGDYAQLLEIKKLYPKTIMSGSGSAFFAIGERISAQDKFWAKNALKSINYGIAEVQSS
                     S"
     gene            complement(51015..51854)
                     /gene="rsmA"
                     /locus_tag="PKI94_02480"
     CDS             complement(51015..51854)
                     /gene="rsmA"
                     /locus_tag="PKI94_02480"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNW25637.1"
                     /translation="MDYLRRAKSFRTKKRLGQNFLIDEKTIRAILESAQISKDDIVVE
                     IGAGVGFVTEQLVKVAKKVIAIELDEEAIKELKKIHCDNLVILHQDILKTDLSLVCDE
                     KVKIVANIPYYITSPIIAHLLGEIDDLDNKNRNKIKEIILMVQKEVAQRIAATEKSPS
                     KQYGLLTILSQFWADCEIIKNVGRKCFYPSPKVDSALVKLIVRKEPKVKLLDYKHFRK
                     TIKAAFSQRRKNIKNCLLSGGFSKENIEKSLKNVKIDLNARGETISIENFGKLSQELI
                     NNA"
     gene            51966..53285
                     /locus_tag="PKI94_02485"
     CDS             51966..53285
                     /locus_tag="PKI94_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNW25638.1"
                     /translation="MLKSKDETILSVFKKGCKPKEEYSIGVEYERLPIHALTYKSVGY
                     EGENGIYELLRAFSKLDNWEYITDDYNIIGLKKGEDLITLEPGCQFELSLKPEKNIAD
                     LKTKIDNFNKKITPLLRRYDINLLEYGVSPVSTYKSIKIIPKKRYHIMANYLWGILSD
                     VMMRETAGIQACFDFSSEKDAIRKFRVTNMLSPFMTAMFANSPIRGSVDTGYKTFRGL
                     SWLNTDSERCGFLSKKFFEKNSDYGFEDYIKDVMKTPMIFIMREGNPVKILGKIDFEQ
                     FIERGFDGFEATLDDFLLHANLCFPEVRLRDFIEIRNHDCANHGMQYAILGIYKGILY
                     SQKSLEEAEELVSNFSYNDIAEFRYNVPKNALETKIKGHFAKDIAKEILKISQKSLKE
                     QGLGEEKFLDSIAEYTLQGLCPADVILKNWYGSWNKDINKLMKYLNN"
     gene            complement(53282..53506)
                     /locus_tag="PKI94_02490"
     CDS             complement(53282..53506)
                     /locus_tag="PKI94_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25639.1"
                     /translation="MGKSKKRILHQGRNNQITKLTREDALGEVVKACNKKRIDEEAKN
                     LISLFGFSAEELLESGARYEDVIVLKGILS"
     gene            complement(53593..54477)
                     /locus_tag="PKI94_02495"
     CDS             complement(53593..54477)
                     /locus_tag="PKI94_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNW25640.1"
                     /translation="MKKALAIDIGGTKIYHTLVSEQGEILGEVNKISTPKDIQELKSL
                     LEEVIKTYEGQVDVVAIATAGAVNNQNNKVISSTANLPKGYNQIDFQSLSTKKVFIEN
                     DANAAAWAEHELGASKNCPYSVMLTLGTGVGGGIILQDRLYKGKSGGAGEMHFKLFAD
                     MRRRCTCGAWDCFEAYASGTGLKLTAEEILRDNNVTTYDVIEGVKKGDTKMKEVFDIW
                     QNHILAGLVGLANLFDPDCFVLSGSLAEFVDTDYLENETNAQIVTAPIKVYKAKAGNH
                     SGMIGAALLALSWIENTN"
     gene            complement(54478..55155)
                     /locus_tag="PKI94_02500"
     CDS             complement(54478..55155)
                     /locus_tag="PKI94_02500"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF002231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HNW25641.1"
                     /translation="MEVLAQLKNKIVVSVQAMPDEPLYKEECMLAMMESVVQGGACAL
                     RVAGTRDTKNAKSFGLTVIGLTKPDKLPDNWLEIVYITPTLEDVEKLIKAGADIVAFD
                     GTFRKRECSVEDIIKKIHSYGKFAMADVSTFEEGLACAEFGADMVSTTLSGYTSYSLT
                     KSDEPDFELLAKLTKKLKVPVVLEGRIWEPKQVQKAFELGAWCVVIGSAITRPQLITK
                     RFLGEKM"
     gene            55201..56535
                     /locus_tag="PKI94_02505"
     CDS             55201..56535
                     /locus_tag="PKI94_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNW25642.1"
                     /translation="MNIKKTLVKYPFLTKEVEIYELENNHKIVLAHKEGGLVNVSSWI
                     KTGSINENDQNNGISHFLEHLMFKGTNKYKAGEFDKILEAKGGIVNAATWKDYTFYYV
                     TLPKGEKDKDLYKAIELHADMILDPILPEEEIGPAFDVNDTKVNEKRERHVVIEEIRM
                     RQDQPWTKVYNACNKAMYTSHPYKRDVIGTPEIISQVTRQDIMDYYKTFYSPQNITTI
                     IVGDFDNKEILNKVISEFQDKWGQRGNLKLTSKPDEPVCETKYLEQKSQINSAFMMFG
                     YLGPLACDLKNSIALEIIAIILGEGTSSRLYQNLIEKQKEPVFNMINADNYQFKDGNN
                     FFIQANFIPEKKDLAINLVKSELEKIVNEEVSQREFLKAKKKIKSLFAHNAETVSEIG
                     ETIGYYMTVCENLALVEEYLNILEKITPKDLKEAARNYLPVSNAVISILMPE"
     gene            56631..57953
                     /locus_tag="PKI94_02510"
     CDS             56631..57953
                     /locus_tag="PKI94_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNW25643.1"
                     /translation="MKLHMQILIALGLGLKRNWHIFFGLIAGIFVGIFMHSHDSVFLT
                     KILTFIGQIFIRLIQMVVIPLVISAIVIGITSIGDNKQLGKFGTKMIIYYGIITVLAV
                     TIGAALALTMKPGVGAAQYINVDAAASIQQQVATTIEQQKGNVLNIFINFIPKNPLES
                     VAKGDMISIIVFALIFAVALAKVGEINRPVVSFFESIFAATMKVTDWIMYFAAPGVFA
                     LTATAVSSFGIDIFQSISKYLAVLCIGFAIQFFVVYPIFLKVFSKVPVLMLYAAIAEA
                     MMVAFGTASSSATLPLTIACCEKRGISHKVSSFVLPLGATLNMDGTALLQTVAVIFLA
                     QAYGIHLGPFLIIQIAFLAMIASSTCAGIPGAGLITIALVLNGLGLSPEQLVAGFAFL
                     FTIERLTDMMRTTLNVTSDAVVAAVIADNENEINYDLLNNPESYKEII"
     gene            57954..58445
                     /gene="queF"
                     /locus_tag="PKI94_02515"
     CDS             57954..58445
                     /gene="queF"
                     /locus_tag="PKI94_02515"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNW25644.1"
                     /translation="MRSKKKLESIKSLGKTKTDYPTEYDPSVLECFENVHPENDYVVT
                     LYAYEFSSLCPKTGQPDFAKLYIKYIPNIKMVESKSLKLYLFSFRNHGDFHEDCINMI
                     MKDLVKLMKPKYFEITGIFTPRGGISITPFANYSNGKEKYNKLRDSRLLEVLKEKTPP
                     SIS"
BASE COUNT        19393 a        10886 c        10311 g        17929 t
ORIGIN      
        1 tttaaggtcc gagtgcaaga tttcatcacg ggtttttatt gttgctcttg taatccagtt
       61 aaaccagcct tttccgtttg aaactttatt ataatttata agtttttgcc ctgacatttt
      121 ttcaaaaccg gctgcggcaa gttttgtaac aaaaccgcca aatacaagcc agtttaaaaa
      181 gcctaaagtg tcttttattg aagcttctct aagttcattt ttgtcgcggg cagaaaataa
      241 tcttgacatt atggtaagcc catagacaag tttaaactgt tctattgtag gagtaaaacc
      301 tttaaattga agttttctga aaatatcgct gccgcttttt cctattgtag ctaaagtgcc
      361 taaagcaaat gccattgcag ttgcgctttt taaaatttta aaacctaaag atttatcggg
      421 ctctcttcct tcaacaccga caaaaccgcc tttaccggtt ttctttttgg tcagatacat
      481 attgaaaggc tgaacgcata ttccgacaaa aacagccatg ccaagcccta atagtgcggt
      541 ttttgtatta agtgccttta atctttttat aaaaacatta tctgcaagag aggatgtttt
      601 aaatgtttca agaattttag ggctttttgc cgctcttgca aatttataaa tatcatcaat
      661 taaagtatcc aaagataaag cttttgtatc taaatctttc aagccatgct ctattgtaaa
      721 tttactttct gcacctgtat cacgaattat aagagatnnn nnnnnnnttt aatatattgc
      781 tttgattctt tacttaattt gcctttttca agttcttcct taagtgtttt tgccacttct
      841 tgctttgatt cttcaaggct tgtccatccg actcttttct ttttatcagg attaaaacct
      901 cttattctgt ctataagttc attcaagtaa ccgtcaatac cttgaccgcc ttcgttgtga
      961 tgtttgtgcc aaagttctgc catgacatca atgttatcgc tatttataaa catcttatta
     1021 acaccgacat ttggaaatgc cttgttaagt ccaaagccta acaacgaagc tgcaacaagt
     1081 ccgtaagtcc ctattaaagt gtgatttgtc gtacctgaca tttcgcgacg cattgtttca
     1141 gcacctgctg caggtcctct tgtaaaatca acaagcgttc ttggaagcac catagcccct
     1201 atatctacag catttgcacc ccaagcttta tttgtgtcca aaaatttcca gaaaacctga
     1261 aaagcaccgt ccacaggccc gccaaatgaa acatctcggt gacgataagc gtttccgatt
     1321 tgtttttgat tattaaattg tgtttggggg atttgtactt tcaaattttt tcctatcgat
     1381 tttttgttaa ttagaagtga aaaaggcttt atatattttt ttgcttgaga ctttgtttgc
     1441 actattcatc ataaaattgg ggtaatattt tttaattgtt gcattatcaa caccggcttt
     1501 ttttaaatcc tcccgatgtt ttttgtcggt ttttcctctg gtataaaaag gaatgaatat
     1561 tcccaataac aacaaagaaa aacctatatt gctaagctgg catattgcac ggtaattttt
     1621 tgcgccttcc aaaacttcgt gtgaagactt taattttgta tcttttgccc agtgtaagaa
     1681 ttttttaata agaccttggc ttttatcggg cttgtcactt aaataattca aaagcttaat
     1741 accttttgtt ttttcaatta ttgtcgctgt tatttttgaa gcatagtctc caaggaagta
     1801 aagccctgaa aaagtaccga tatcccttac ggtagattcc ttaagctcat cttcattttc
     1861 cgaagaagca attctgcttg aaaaagtaga agctgaaatt aatcttgcct gattcattgt
     1921 agggaaaaga ccgctaaact gaaacatctg cagactgggc tttttaagca ttgcaagaag
     1981 cgcaacacct atcatggcac ctaccgaagc aattttgcca ctgaagagtt tcattttttc
     2041 ttctgcggta agttctttat gctcggcttc tttgaagtct ttgtatatcg gagcaccttt
     2101 tttaccggat attttagaag taatccacct gtttaaaggc tgcattgaaa ttgcaagcgg
     2161 aataattata ctcaatccaa gcacgctttt tgtgttaacc aaacgtcttg cgctgttttt
     2221 aaaccagctg agatctttgg cttgggggtt ttcaatcaac tcttgcaata ctttaggaat
     2281 atcacgcatc acagaagaaa gattttgact gaaatatttt ccgtctttgc ttcctatttt
     2341 tatgttttca cttatatgag tttgatttgt aatgtgcccg aaagcctctt ttataagctt
     2401 tttaactgct tttggatttt tctcgtttac ggcttgagcc aaaagtttta cgcttttttc
     2461 aactaaagaa tcatcattaa atgacttaag aacttcaccg tcttttccgt gaatatcctt
     2521 taaaagggtt tttattgtat cttctacttt tgaagttcct tcaccttttg cattttccca
     2581 ataatgtgca accgtattta tagtctcttc gtttgcccaa caagaaccca tttttgtttt
     2641 gttgcccata gtgacttttt caaaaagttt tgcaataccc aaaacaataa aactaggtaa
     2701 aaggcagttt acaataagcc ccgaggactc ccttctgaag gcttcaaagc ctgcaaaaat
     2761 atttgttttt gtttctcttg ccgttcttgg aataatgtct gttgaaagat ccagtaccgc
     2821 aacattcacc atggggtttg tttcgcaaac ctgtattcct cttaatatcg catcgccaag
     2881 ccccccgaat tgtgggtttt tacttggggt tgatatcttt ttattaatgc tattagtgtt
     2941 aattttttct atcattttca aattttaaac cgatacacac tacaatgata acagtttgat
     3001 gcctatttta aaagtgctga attttaaaaa ttgcctaaaa aagtcctttt gtaaagttta
     3061 tattaagaca aattttgaat aattttctaa aaatagaaca ctttaaaaag gtttctaact
     3121 tttgaactaa aaaacgataa aaataaaaaa ctaaaaatac caaataacag attttttaat
     3181 gagcaaattt ttgtaaattt ttgttaagag aaatagagcc cccacctgaa ttcaaataaa
     3241 aacaagggga ttatttgtca attttttaat aaacggatgt attctttttt aagaaaatct
     3301 tttcctggcg gaatttgaat aaaatcccat ggcaaaattt cttctgaatt atactctctt
     3361 aaagcgtaaa aatcactatc gggcaataat tttttcttaa caaactctct gtaagaagat
     3421 ttaaacgcac cgatgttgcc gcccagttca taaactctaa ccagataatc tgttaaatct
     3481 cttgagcctc tggataaaag cgtttgccag taatcccatt tcgggcttga aaaacgggct
     3541 tttatgccga gtttgtgaaa ctctttttta agataattta tttttttctc aagagttttt
     3601 gtatcatctc ttttgcacca ttgaaaaggc gtatggggtt taggcacaaa agaattaaaa
     3661 ctgaaagtca ggtcaaatcc tttattttcg gctttaagtt ttttggcaag attaacggtt
     3721 tcaatcaaat cttcttttgt ttctgtcggt aaccctatca ttgcatatat ttttagacct
     3781 ttaagcccgt tttgccttgc cgtttttgct gtttgaagga tttgttcttc actcaggttt
     3841 ttattaatta ctttcctgag cctttcactt cctgcttcaa tagcgatagt tgtatttttt
     3901 tgaccgcatt ttacaagggt tttaacgaca ttcggggcta tcgagtctgc tctgagggaa
     3961 gaaacgctca tttcaatttt tttatcggag gctttaattg aaatatattc gcatatttcc
     4021 gtaaatcttg ggtgtgcact tattaatgcg cccaaaagtg ctattttatc ggtattttca
     4081 agccctaaat cgattgtgtt aataatttct tcatattcaa tgaatcttgc gggaagattt
     4141 aagtatgagg caaggcaaaa accgcatctg ttggaacatc cgcgggctat ttctattata
     4201 aaagtgtttt tgaaaaaact tttttcgctt aaaatcggtg tagaaatgca ctgagaaagt
     4261 tcacaatggg attttttaac aaacttttgt tttaaagaag gaacgtaaat cccttcaatt
     4321 tcagaaagtt tttccagtat tttggctttt tcaagtgatt tattttcctt gcagacctga
     4381 attgctttta tattcgtttg ctcgccgtca cctattatga tgaagtcaaa aaactcgcaa
     4441 aacggttcag gattagcact taaaacgggc cctccgccaa aaaccaacgg atgagaagaa
     4501 tttctgtctt ttgccttaaa aggaattttg tatttttgga gtattttaaa aatattcaaa
     4561 aaatccaaat caaaagaaaa tgaaaaaccg attaagttaa tatcttttat cattaaagaa
     4621 gtctttttag tatctgtaca aaccatctcc acattaacat cttcaacctc gtcaatggtt
     4681 ttaaaaagcc acatgtaacc caaggaggac agggcaaaag aataaatacc gggaaacgcc
     4741 atccatacat taaaatttgt ttgtttattt tttaatctgt tatataaaaa tttttcgctc
     4801 attcttgtcc gttattaact gttttcaagt atatttcatc aaatagtacg cagattatgg
     4861 ctgcaatagc aggtgccaaa attacacccc aaacccccaa aaattgtgct gcaacaagca
     4921 gcgaaaagat tattacaagc ggatgcaaat ccaaaaaacg cccaaaaaca accggcctta
     4981 caagattatt cgatacccac tgagctgcca aaaacgcaaa aataacaagg gcaattccaa
     5041 ccggtcccat ttgatagcac attatcaaac aaagccctaa cgcaatggta ggcccgataa
     5101 tcggtataat gtctaaaacc cctgttatca aacctaaaag cagtgcataa tctactttta
     5161 ttaaaaacaa tgctactgcg gtaattaaac ctattgcaat catattcaaa atttgggcta
     5221 taacatatcc gccgacttta tgagaaatat tttgtgaaat taaatctgct ctttctttta
     5281 tcttatcggg gaaaatactt ataaaactct ttttaatttc gtctttgtct gccaaaagat
     5341 aaaatacaat cataaatatt gcaagcaaaa agattatacc ctgagtaatc cccaacgtta
     5401 aatttattga ctgattcaca accccgcttg caaaaggcga agaagatttt actattgatt
     5461 caaaattcag aaactccgaa agcttatgtc cgtaaaattc atgcgtcatt gtaaaatctt
     5521 caagtaattt caattgcgca ggtaatgcat ttacaaagcc tttaatttgt tcaatcgcta
     5581 ccaatacaat cggaacaaag catgcaaacg aaattattat agttccaaaa agtacaatgc
     5641 ttgttgctaa agaccttttt attttcttag aaagcctgtc aacaagcgga ttaagagaac
     5701 atgcgattat gtaagatgca aaaaataaca ttgctattgc ttttatctgc gaaataaatg
     5761 taagcgtaat taaaattaaa ataaaaaata ttacgttttt tgtcgtcatg tattcctgaa
     5821 aaatcatctg taaactcctt actgcttaat atttttatct atctcttcct gaacaaacaa
     5881 tgcctgatta atatcggtct gtgtcacaat cttcattccc aaaagaattt caccaagctg
     5941 tatatcttca aatttttgga tatcaagtgc cattcccaaa tgcacaagac ttaatttccc
     6001 actttgcata aggatttcac cgattttttg atgaatttga atatcagacc attcattaat
     6061 atcatataaa ttcttcatga aaatattata acagaattat ttaaaattaa taccaaatga
     6121 aaacttaata ctacccaatg ttagtattat ttgtaaataa atgtataaaa taaccctcaa
     6181 aggggttgtg ccgaaataaa ataacaagta tacttagtac acttattctt ttctactaca
     6241 aatgtaaaat ttcgtaaaga tgaaggaata acatggcaat taatacccta agcaaaccct
     6301 ttatttaaat ataagcaata gtgtgctgtt gccgacatgt gtaaagatta taggtatctt
     6361 cggagaaggt taattacatt agatgaacgt taattcaatt aaaaattatg attttaaaaa
     6421 tcatactaat atttcattaa atgccccttt aaaaaacggt gcttccaaat ctaaaatttc
     6481 ttttaaaggc ggagaatata atttggctaa tatgatgggt cttattgaag gactgggttt
     6541 ttttggtgcc ttttttatca tagatagcct tagtctgatt acacctagaa ttgttgtcgg
     6601 tttaaaccga gacaaagata aattaggaca cttaaattac aaagcaggag ccgaagaagc
     6661 cggaagagaa gttctttccg gtcccagcat gtttttaatt cctataggaa tttttgaagt
     6721 cgtaagaaga actgctgctg cttctaaaat cccaaaaaat actatgaaag ttttatttga
     6781 acatacaaaa caaactttaa aggggcttgg cgatacttct ttgctgtcaa ataaaaaaga
     6841 attcagtgaa aaatttgcac aacagttatt tgataaagca tttggtgaat ttgacttagg
     6901 cgataaagcc gctcaattca aaaaacgttt tacaacaagg cttgtttggt gcgcaaattc
     6961 aacaagcgat aaaaaaagct tcaagaaaaa tatggcggca tttgaaaaaa taatatctga
     7021 aattaataat tcaaaccgct ctaaaactcc gattcaccca aaaataattg atataggcga
     7081 caatgtaaaa gtaagagcaa aagacttatt tgaagacttc agacattatt caagcgatat
     7141 aattacaaaa atttcaaaac gtaaatggat agatgatgct tctaaaagcc ttaaaaaaga
     7201 ggctgttaac tggactgata acatgaaaaa aatcaggcta agtttgcgct ttggagcttg
     7261 tgtagcatcc ttctttgccg taggggcgtt tttaattcac ctgccaaaac tttatcaagt
     7321 tagcaaagat tcttctccgg cagaagaaag tgctaaacgc gcaagcttag aaaaatcaaa
     7381 aggagggact aatgcaagtt agaaatatct ctcctgagat tatcaataaa aatatcaaca
     7441 aaacaagcca aaacccaagt tttggaaata ttctgacaaa atcacgcaaa gccgttttgg
     7501 acttattcga acccggaaga gccggtgaaa tgggtatgaa gttgtttgtt ataaatgctt
     7561 acgtattctt gctcagtgga agacttttga gttctcgcga caaaaaaggt gaaaaaactc
     7621 agaaagaaac tgcaatggat tatatccgta acaacaacga aaaaagagaa acaatcataa
     7681 gagacgtccc aacaattctt cttgccgtaa tgggagttcc ttggatagaa aaatttgtct
     7741 ctaaaagatt acaaaaatcc caaggttttg caatacatga cattcaaaaa gataagttgg
     7801 gcaatattat tccaaagtat caagtcggcg actggtatat atttgataaa aacttaaaaa
     7861 ccggcttcaa aggttttacc caaagattag ccgatatggg cggaaatttg cacaaaattt
     7921 attctcattt agatgaaggc ataaaagata aaatcaaagg ctttagctta caaaacgatg
     7981 aatttatgaa tcaacttttt gcagataaag agttaactaa agaagttgaa acagcatttt
     8041 caaaccccgg caacaaagct ttcaggcaag caggatttct taaagccctt cctaaagtct
     8101 tcggctttgt ctcaacactg ttgctgatag gaatacttat tccaaaattc aatattttta
     8161 taacagaaat gcttcataaa ggcaaaaaac aagaagttgc tcaagaaaac aaagataaca
     8221 atcaaaagcc ggctgaacaa aaacaaactt caacaaatct gttggaacat gctaaaaata
     8281 attcaaatac atctaatgta aaaccaaaca tataatctta aggcggcgca aagatttgcg
     8341 ccgcctcttt ttatttttta aactatttac tttgtacaaa aacagttcaa atttacctgt
     8401 tctttaagta tttgagcttt atcggtattt tcccagggaa cttcaaaatc cattctgccc
     8461 atatgaccgt aggctgcaag tttttggtaa aacttcccgt tatttttggc aggaagtcct
     8521 cttaagtcaa attgattaat gattgcagca ggtctgaggt caaaattctt actgataagt
     8581 tcacagatat catcgtcaca aatttttcct gtaccaaagg tatcaaccat tattgaaaca
     8641 ggttctgcga cacctatagc ataagccaac tcgatttcgc atttttgtgc aagtcctgat
     8701 gctactatat ttttagcaac ccaccttgcg gcataagctg cggaacggtc aacctttgta
     8761 ggatcttttc cggaaaacgc accgccgccg tgtcttgaat atccgccgta agtgtcaacg
     8821 attatttttc ttccggtcaa gccggcatcg ccttgaggcc caccgattac aaaccttccc
     8881 gaaggattta taagatattt tgtatcagaa tccggtttta agctttcgtt ttcaaatacg
     8941 ggattaataa catattcaat caaatcattt ttaattattt gctgaatttt gtgattatct
     9001 tcttcgccgt taattacagg gtcgtgttga gttgaaagaa gaaccgtatt aatccttgaa
     9061 ggctttcctt ctgtgtattc aactgttacc tgagacttgc cgtcgggtct taaatatttc
     9121 aacagtcctt gctttctgac ttgtgccaat ttaaaagaaa gtttgtttgc aagacttatc
     9181 ggaagcggca ttaactcagg ggtttcgtca caggcaaaac cgaacataat accctgatct
     9241 cctgcgccca agttttctgt ttccatattt gaagtgtctg cattgtcatc tcttgtttca
     9301 aatgctttgt tcacaccacc tgcgatatca caagactgct cgtctatgct cgttaaaacc
     9361 gcacaagttt tataatcaaa acctccgcaa ttttcaccct catagcctat gtttttaatt
     9421 gtatttctta caacctgagg aatatcgaca taacaatcag aggttatttc tccgcacaca
     9481 agtgccattc ctgtagtaac cgtggtttct gctgctactc tggatttagg gtcttttgtc
     9541 aaaaattcgt ccaaaatcgc gtctgaaatt tgatcgcaaa ctttgtcagg gtgtccttca
     9601 gtaactgatt ccgaagtgaa taaaaaattt tttgatgcca tttttttctc cttaattttt
     9661 ttactttgtc ggtaagtttt taaatcccaa cccgacacta tcctttagat tctatgtgta
     9721 aaatctttga taaaatagta gcccaaaaac atttgaaaat caaatttttg aggtaaacaa
     9781 caaaatcatt atccgagttt cttaaagtta tttaataata tttctttgcg cttccccttg
     9841 tgttaaaata tttttaaagc ggggaacaaa cttggatagt aaagaaaaaa ggtttagagt
     9901 atttgaccaa aggctggggt gttggaacat ctgttttgct atatttgcaa taatcttaat
     9961 tatttatctt ttttgtattc aagtaatcga cataaaacaa tacaggatta aagccaaaaa
    10021 acaaagaagc tcaaaaaact tcattatgcg cggagatatt tttgacagaa acggggtaaa
    10081 acttgcctct gacaagattt attatgatgt ttacgctcac cctgcagatt atgaccattc
    10141 gcccgaagaa ttagcaaaaa ttcttgcccc ttatttaaaa atttcgaccg ctacacttgt
    10201 cgaaaagctt tccaagcaag attctgtaat aactttgaaa aaagacttgg atagaaatgc
    10261 cgctcaagcc attaaaaaac tgggattaag agaaataagc ctgggcaaaa aaaacacaag
    10321 ggtttaccct caaggctcaa tggcggctca cgtcttgggg tattacaatt ttgatgccga
    10381 tatagcatca ggaattgaac ttacggcaaa agaccaatta gagcatgtcg aaaaaaatgt
    10441 tcaaaccgaa aggacacctg acggaaatat aatttacagt ttttcaacgg atcctgttgc
    10501 aacttcatcc ccattaaaag gcaagagttt aactctcaca attgattctg caattcagca
    10561 catttgcgaa caggaacttc acagaatgat tgcaaaaaga agtgccctaa gaggtacggt
    10621 tattgtgatg aaccctagaa acggcgaaat tttagcctat gcggtttatc cttattatga
    10681 ccctaataat tacaaaagtg ctacacagcg acaaataaaa aactggaccc tgaccgatgt
    10741 tttccctccc ggctctactt ttaaaaccat aacggtaacc tctgctatgg cattgggcaa
    10801 aatcaatgaa catacaacag tttacgattc gggcaaaatc agagtcggct ggtgggaaat
    10861 caaaaactat gactactcaa aaagaccttg tccgggtgta attaatcttg aatatctttt
    10921 tgagcactca agcaatgtcg gagcggtaag gattgctcaa aaaatgacct cgcaggaata
    10981 ttattcaatg cttacaaaat tcggcatagg ctcaaaaaca ggcattgatt tacccggaga
    11041 atcaagcgga cttttaccta aatggcaaaa atgggatcct tcaacacatg cttcaatggc
    11101 atacggttat gcggcatctt taacccctat tcaaatggtt tcggctgtct ctgcgcttgc
    11161 aaacgacgga atcagagtta ctcctcatgt aataaaatat tcgccagaag aagccgagac
    11221 aaaaattttc aaaacagaag ttatgccgcc tgaaaccgct cgaacaataa ctaaaatact
    11281 taccaaaagt attgaaaatt caaaatcacc gataaaaatg gatgcatatt atatcgcggc
    11341 aaaaacagga acttcaagaa aaccgaacga aaatgcagca ggttattcaa acaaactcta
    11401 tacctctatt ataggctacc tgccggcttc aaatcctcag gtattgattt atgtactggt
    11461 agattctgca gcaggcggcg aagtctgggg aagcacaatc gcagcacctg tttttaaaga
    11521 aatagcaagc caaagcgcaa gaattttaaa tcttaaaccg gataaatcca aataaattta
    11581 ttttttaata ttttcttcca ctatcatttt taatattgcc cttaaagact ctttagaaga
    11641 tgcaataaat tcagactctt tttctccttc aaatgcttcg tctaattgtt ttttaatggt
    11701 ttccattttt tttgtatatg cccaaacttg tggacaacgc gattctcttg cttcttttga
    11761 gtttaaaaat ttatcgtaat aagaatcgcc ttcagacaat atttcttgga aaatatcgca
    11821 atgttcttgg gaatacacat aaaaagtgtt aagttttgaa ttcatagggt gtttaatgtt
    11881 cggattaata gtatttatca catactccat tggaaaagta ttttcaacgg catcttctaa
    11941 gtatgaccaa agagattgct ccccaactac agaaaaatca ggcaagaaat ctgattccaa
    12001 aactttttgc aatcgattat gcctaacagg ctcaattaaa ctataaacac cgtggcgaag
    12061 aacaaatttt tttatttctt caaccatgtt ttcttttata agctctctta tatattgctc
    12121 tttgtttaaa agactgggcg aaatactatt atttgcatca tcggtaaaac atttttttgc
    12181 aacggttact ttgtaaattt gtttaaatgc gggttgattt ggcttaaatg tagtattttg
    12241 tattctcatg tttgtgcctt tattctgtca tgttaatttt ctataacccg taaaaaaaat
    12301 tttttgttat ttcgtaaaag aattgttaca aaaaattttt ttcaaatttg tgctataatc
    12361 ggcttgaaat atcggacaaa attaggagtg gagattttaa atgaagctga gtgatttaat
    12421 atcaaattta caatatgaag agaaattaaa ttttgaagat gttgaaataa ggggggtttc
    12481 ctacaattca aagacaacaa aacaaggtga tatttttgtt tgtcttgtgg gcgaacactc
    12541 cgacggacac caatatttta aaaacgcaca agaaaacggc tcaagcgcct ttttgaccga
    12601 aaaagaactt gacacgaaac tgccgcaaat tatcgttgat tcaacaagac aaaaaatagc
    12661 agatatcgca gataaatttt atgattcgcc ttcaaaaaaa ctaaatttga taggaattac
    12721 cggaacaaac ggaaaaacaa cagtaacaca tttgattcaa aaaatatttg aggaaaataa
    12781 acagcaatgt gctcttatcg gaactttagg ctacaaattg acctccgaaa gcgaatacaa
    12841 agaagcaaaa catacaactc cccaagcacc tgagcttcaa tttacgcttg atttaatcgc
    12901 tgacaaaatg aaaattccaa actgcgttat ggaagtaagt tcgcatgcac tggagcaaaa
    12961 ccgagtgggc ggctgcgttt ttaaaggtgc agttttgaca aatttaactc aggatcattt
    13021 ggattatcac ataacaatgg ataattactt tgaatccaag gcgattttgt tcaaagaggt
    13081 tggcgaattt gccgtaatta atgtcgacga tttatatgca aaaagatttc ttgaagtaat
    13141 ccccgaagga gttaagattt tgacttacgg gatagaaaac agtgccgata ttatggcaaa
    13201 agatatcgaa ttttcaccaa aaggcgccga atttgccctt gaagggggca aattcggcgc
    13261 ccaaaacgtc tgtttacaaa tgaacggaat gttttctgtt tataacgttc ttgccgcaat
    13321 tacaacaggg cttgcctcag gttttgaact taaaagctgc atcaacccgc ttgaacaaac
    13381 acaaggagtt gcgggcagat ttgaagttgt agcgcaaaac ccgcttgtga tagttgatta
    13441 cgcccacacg ccagacggac ttgaaaatgt cttgaaagct gcaaaagaaa taacacccaa
    13501 aggctctgag ttaatttgtc ttttcggctg cggaggcgac agggatgcga caaaacgccc
    13561 gaaaatggga gcgatagcag aagcactttc agataaagtg gttataactt ccgacaaccc
    13621 cagaagcgaa gaacctcaac agattataac agacattctt gcaggactta ggtctgtgac
    13681 ttccgaaaaa gtttttgttg aagcagaccg cggcagggct attgcgcttt tgaagacaat
    13741 atgcaaaaat aatgacgtaa ttgttattgc aggaaaagga cacgaagatt accagatttt
    13801 aaaggataaa acaattcact ttgacgacag ggaagaagca aggaaaatat ttaatgtttg
    13861 aacactttta taaatcaaaa ctgcttgtgg agcagcattt tcacggagct ttcggggtag
    13921 atttttccgt ttgtgaagcc gatgaaattc tttttcttgc aaaaaaactt ttgcaaaaag
    13981 gtatcggcgg tttttatccg acacttgtca cagacacggt tgaaaacatc aaacgccaaa
    14041 tcggtcgtat aaaaaccgcc tcgcaaaaac aatcttgcga agaggcaaaa attttaggaa
    14101 ttcaccttga aggaattttt ctcaatcccg aaaaaagcgg aattcacgac aaaaacttat
    14161 ttttgaatcc gactgtcgaa aactacaagc tcatagaaga tgattttata aaaatagtaa
    14221 cccttgcacc tgaattagac aaagactttg aacttagcag atacttaaaa ctcaaaggag
    14281 ttaaagttca ggcaggacat tgtgtgggct cagacttaag ctattgcgac ggagcgacgc
    14341 acctttttaa cgcaatgtcg ggtgttaatc accgggaaaa ttcgaccgcg cttagcgcac
    14401 ttgatgacga caggatttac acagaaatta tcgccgacgg aattcatctg agcgaagata
    14461 ttttgaagtt ggttttcaaa acaaaacctc atgacaaaat cattttggta tcagattctc
    14521 ttccgattgc aggaagcgat ttaaaggaaa tggcattcgg aggtaaacga attttttacg
    14581 acggcaaaaa agcgacttca aaagaaggta cgcttgcagg aagcaccgct tttttggatg
    14641 aaattgtaag acatttgtca ctagaaaatc ccgaaaattt tgaaaaatac gtaaaaatgg
    14701 caagcgacaa tctgtataaa tatcataata tcgaacttga cggatgtgta ttttgggatg
    14761 aaaatttcag aataaaggcg gtagaaaaag atggagtcgt actatataaa aataattaaa
    14821 tattattgtc atcccgaact cgtttcggga tctatcccac atactaagct ggaaagatgc
    14881 tgaaacaagt tcagcatgac agattaaaaa agggaataaa taaaatgaaa agcgatgaaa
    14941 taaaaaaagg tatatcaaga gcacctcaca ggggcttttt gtacgcaacc ggcgtcagca
    15001 aagaagggct taaaagacct tttataggaa tcgccagttc gttttcggac ttagtcccgg
    15061 gccacatcgg aatgagagat ttggagcgcc aaattgaaaa aggaatccac tcgggcggcg
    15121 gtcaatcgtt tattttcggc acccctgcca tctgtgacgg tcttgcaatg ggacattcgg
    15181 gaatgcgcta ttcgcttccc tcgcgcgatt taatagccga ctgtgtggaa agcgtcgccg
    15241 cagcccacca attagacgga attgtcctcc tttcaaattg tgacaaaatt acacccggaa
    15301 tgattttggg tgctttgaga ctaaatattc cagccatagt agtaacggca gggcctatgc
    15361 tcgatgggtg ctcaaagggc gaaaaactta caatgataag aggttttgaa gcgataggaa
    15421 aatgcgcaaa cggagaaatt tctcaagaaa gactttttga aatcgaagaa aatgcctgtc
    15481 catcggcggg ctcttgtcag gggctttaca ccgcaaatac tatggcgtgt ttgaccgaag
    15541 ttatggggat gagtcttccg tattgcgcca cggcaagtgc tgtttcttcc caaaaacgca
    15601 gaatagcctt cgaaagcgga atggcaattg ttgacatggt caaaagcagc gtaaaaccgc
    15661 ttgatataat aacaaaagat tcaatccgaa acgctattgt aatggatttg gcacttggcg
    15721 gctcgacaaa ttctgtcctg caccttcttg ctattgcaaa tcaagctggg gtaaatatat
    15781 ttttggatga ttttgaaaag ttaagccatg ttgttaatca aataatcaaa cttgaccctt
    15841 catcaaccac aactatgagc gactttcaca ttgcaggcgg aattcctgcc gcgctaaaaa
    15901 ctttgtgggg caaaactccc gagttaaaaa acacaaaaaa ttgtgtgggg ctttcggtcg
    15961 aaaaaatcgc caaagccgcc tgggttgatg aaaatgtgat aaaacctttt aatgctcctt
    16021 tgaccaaaaa tgcgggactc gggattttgt acggcaatct tgccacagaa ggtgctgtga
    16081 taaaaatttc gggagtggat gaaagctgtt ttgaatttga aggaacagca atgccctatg
    16141 acagcgaaga agaagcaatg aaagcacttg aaaataacca aattaaagca ggacacgtta
    16201 tcgtaattcg ctatgaaggg ccaaaaggcg ggccgggaat gagagaaatg cttgctccga
    16261 cttcgcttct tgtcggaaaa ggtttgggaa aaaccgccgc attgattaca gacggaagat
    16321 tttccggtgc cacaagagga atctgtgtgg gacacatttc gcctgaagcg gcacttggcg
    16381 gacctttggc tttggttaaa aaaggtgata aaattaagat agatatcaac aaacgttcaa
    16441 tcgaactgct tgttgatgaa gctgaacttt tgaaaagaaa agccgagctt aagccttttg
    16501 aactgcctca aaaaggctac cttgcaaggt atgcccaagc cgtaaaaagc gcaagcgtcg
    16561 gcgcgataac cggatgattg tcccctcgcc ccttggggga gagggtgccc gtagggcggg
    16621 tgaggggtaa aaaatcgaat gaaaaaatta atcgactata aagaagaaat acaaaaatgt
    16681 tccaagtgcg ggctttgcca gtcagtatgc cctgtttata aagttacggg taatgattgc
    16741 gccgtttcgc gcggaaaatt cataatgctc ggcggtatta taaagggcga tttgaaactc
    16801 aaaaaaaacg ttaacaaata ccttgatatg tgtttaaaat gtaacgcatg caaagacttt
    16861 tgcccaagcg gaattgacgc aagagaaatt tttacctgcg caaagtgtga atattttgag
    16921 ggggcgaatt caaaatttgt taaaattttg aattcgcccc ctgtgtttaa tttgttttta
    16981 aattctgcaa aaatctgtac aaacacttac cgttttttga agtttgacag gattttaaaa
    17041 ttgttttatc cgattttgct gaaatcaaat ttcggcaaaa gagcagtttt agcaaatgaa
    17101 tttattgcaa aaaattcaaa tatattaaca aaatgcacct attcacctat tcacctattc
    17161 acccattcac ctaaaataat ttacttcaaa ggctgtgtga atgaatattt aaacccgagt
    17221 gttaaaaatg ctgcagaaaa tgtgctcaaa aaaatgggtg taaatgtatt agatattaaa
    17281 tttcagtgct gcgggctgcc ttttttgtct gtgggaaatg ctgaacagtt taaaaagcag
    17341 gcggagttta atttggcaca aatccccgat gattttgcca atgagtttga ttatttctta
    17401 accgactgtg caagctgtca ggatgctttc agagagtata aaaaagtaat tgatgatgaa
    17461 aaattaatgc aaaaattgga caaaatcctt gaaaaatctt taaatataaa cgagtttatt
    17521 gtaaaaaaca cccaaaaatt tgtatttgac aaaaaaatca aaataacttt ccacaaacca
    17581 tgccaccttg aaaacattga ttttttgcaa gaatttttaa gcaaagcaga aaatgtcgaa
    17641 tatattgaga tgaaagaatt tgactcctgt tgcgggtttg ctggtgcttt tgcgctaaac
    17701 aacgataaaa tttcaaaaga aatttcttct aaaaaagcta aaaatgcaat cgaaacagaa
    17761 gccgattaca ttttaacatc ctgcccttcg tgtgttttag gcttaaaaca aggactgata
    17821 gaaaacggaa aacttattcc tgtattaaat tttgtagaat ttatccaagt ctttataaaa
    17881 tctttacatt agttaaacta tttacatata aatctactaa ttgtagtatg atatagttat
    17941 agaatgtaat tcttggagga attttgtccg aaaagttcag aataaaaggc ggagtgccct
    18001 taaagggaga agtgtcaatc ggaggggcta aaaatgcagt cctgaagttg atggctgctg
    18061 caattttagc aaaaggtgaa accaaaattt ataacgttcc cgacctcaca gacgttgaga
    18121 ttatgtcaag tgttattgaa ggtttgggcg caaaaatcgt ttacgacaaa acagaaaaat
    18181 caatcagcat tgatgccaca gacctttcaa gcataaccgc aaaatacgaa ctggtaagca
    18241 aaatgagagc gtcatttata attttaggtg cgcttgtttc aaggtgcaaa gaagcaatgg
    18301 tcgcgcttcc gggcggctgt gccataggag agcgcagagt tgactttcac ataaaaggct
    18361 tagaagccct tggcacaaaa ataaaaatcg aaaacggata cgtgcatgca aaagtttcaa
    18421 agctcacagg agcagatgtt taccttgata ttccctcagt cggtgcgaca gaaaatctta
    18481 tgctggcttc tgtttttgca gaaggctcta caagaattca aaatgcggca caagagcctg
    18541 aaataattga tttggcaaac tttctcaaca caatgggtgc tgatatttca ggtgcaggaa
    18601 cctcagaaat tgttataaac ggagtaaaag aaaattccct gagacccatc gaatacacca
    18661 caattccgga tagaatcgaa gcgggaactt atatgagcgc gataattgcg acaaaaggaa
    18721 aagggattat aagaaacgtt tttcctgcgc atttgacttt ctttaacgac aaattgctca
    18781 aaatgggtgc aaatatcaaa ttaatcgaac ccacagcact tgaagtttcg tgcaaaaacc
    18841 gcttaagttc gataaacttt gtaacccagc cttatccggg ctttccgact gaccttcaat
    18901 caatggcaat ggtgctttta tcaaccgccg aaggtgtcgg agttattaca gaaagtcttt
    18961 acgaaaacag gtttatgcag gttcccgacc ttgtaagaat gggtgcagac attcatcagg
    19021 acagaaacca tgctataata aaaggagtta aaaaacttgc aggcacagat gtttcggcaa
    19081 gtgatttaag agcaggtgct tcgcttgcag tagcagggct tatggcagaa gggattacga
    19141 ctatcgaaaa ccttcaccac atagaccgcg gctacgaaag atttgaagaa aaaataaggc
    19201 tgcttggcgg caaaatcgaa cgctacaacg acgaaaaccc gccggcaaaa ctaaaagaag
    19261 gattgtataa tgaaacccat ttataaaaga tggtacgatc atgatccgct gttgcttgaa
    19321 gtcattgaac ttctgagaaa ttatcaggaa gaattgaagg ctcaggcaga agtttttcta
    19381 aaaaaaatcg aagaaaaggt ctcaaaagat gctatcgaca gattttatat gcttgtaaaa
    19441 cctgttaacg gcaaccgctg gtacgaccat gaccccgtta tctcaaaaac agtcgaactt
    19501 ttaagggttg ttccgcccga tgtccaaaaa agctgcgcaa aacattttat tgattcactt
    19561 aaagaaatag ggattgaata cgcaagtcct aatttaaaat aatgcttgat tttgatttcg
    19621 ataataaaaa tttctcccgc atgaagcgca atcttgcagc ttcaaagtct tttgcaataa
    19681 ccggtttaac ctcttttttg cgcctgcttt tagtgcaaaa aataaaagaa ttttcggaca
    19741 aaaaagtcct ttttataaca gcaagcgaac aaaccgcact caaatgccaa aatgacctta
    19801 aaaacgcttt tgaaattgat gccgaaattt ttccgtttca aagcacttcg atgtatgaac
    19861 ctgtcagtgc aaatctttat gattatgccg cgcagattga gctgctcaat tcagataaag
    19921 accttattgt gcctgtcaaa gcacttttgg aaaaattccc cgacaaaaaa ttctttgaaa
    19981 aaaattcaat ttatttaaaa atcggcgatg aaattgatac aaaaaaattg gcgcaaaatc
    20041 ttataaattt aggctacaaa cgctcaacta tggcaagcga tatgggtgaa ttttctataa
    20101 gaggcgatat catcgatgtt ttttcgcttt cacaaaaccc tttcaggata gagctttggg
    20161 gtgatgaaat cgtcgatttg agaatttttg acaaccaaac ccaaaaaagt atcgaaaaag
    20221 ttaaagaaat aacaatcaaa cctgtttata aattcatatt tgataaatca ataaataaac
    20281 gtcattctga acttgtttca gaatctcatc cgctttcaga aactaaaata aaactggaag
    20341 aaggatattt tgaaggaata gaagtttatc aaaattattt caatcctgat ttggtgagta
    20401 tttttgatta ttttaacccc tcacccctac cctctcccgc aaggggagag ggagaacaat
    20461 tatcctctca cccttcacct ttcacttctc acttaattgt attcgacgag atgtcagaga
    20521 ttttttccaa atacgaattt atcgcacaaa attatcaaaa ccagctgggt gaaaatatta
    20581 aaacggggct tatcttttac ccctcacccc taccccctct cccgcaaggg gagaggggaa
    20641 aaacagaatg tccaaaaaac tcaacccttc gccccttgat ggaggagata aatgtttccg
    20701 atattccccc tacccctcgc cccttgcggg agagggaaga ttttcaatgc gaggaacgag
    20761 cattagaaaa tcgggtgagg ggtaatctta aaaatcattt tgaattggaa gaatttcaaa
    20821 accaaaccgc gggttttgtc aaaataggat ttaacaattt tatagactac gactgcgggg
    20881 atttaaccga atttgattcc cagcccctga aaggttattt ttccggaatt gacgaaataa
    20941 cagatttcat aagctccaaa aaaggctata aaattctggt cgcaacagat tttccggagc
    21001 gcgtgaaaga aatttttgct caaaaaaatc tttttgattt tgaaacagca aaaagtcttt
    21061 cctgttcggg ctgcattata gaagacctga agctgatttt gcttacagac aaggaacttt
    21121 ttaacaagcg tccaaaagaa attaccgcct caaagcgtgc ttattacaaa gaaaagcccg
    21181 aatttatcga aagcataaac gacattaaag aaggcgagtt tgttgttcat tcaatccacg
    21241 gggtgggagt ttacaaaggt atatcacaac agcagcttga cgggcagtac aaagattatc
    21301 tgacaatcga atatgcgctg ggcgataaac tccacatgcc ggcagaacaa attaacctcc
    21361 tttgcagata cagaggctca ggtacgctta aacctaaact ttcgcgcatg ggaggaagcg
    21421 attgggaaaa caccaaaaca agggtcaaaa aagaagttga aaaaattgca tacgaccttt
    21481 tgagactcta tgcaaaacgc aaaatacaag aggggatagt ttttgaacaa gataacccat
    21541 ggcagtttga aatggaagaa gcctttgaat acacagaaac tccggaccag atgaaatcaa
    21601 ttcttgaagt aaaatcggac atggaaaacg aaataccaat ggacaggctg atttgcgggg
    21661 atgtgggatt cggcaaaact gaagttgcta taagggcaat atttaaagcg gttacttcag
    21721 gctatcaggc agctgttata gtccctacaa caattcttgc gcttcagcat tttcagacga
    21781 ttcaggaacg ttttaaacct ttcgggataa aagtcgagct tttatcgaga tttaaaagcg
    21841 caaaagagca aaaacaaatt ttaaaagagc tcactctggg cagcattgat gtggtaattg
    21901 ccacccacag gcttttgcag ggcgacgtcc atttcaagaa tttaggacta ttggtaatcg
    21961 atgaagaaca ccgcttcggc gtcagacaca aagaaaaact taaatcaatc aaggaaaaca
    22021 tagacataat aagcatgtct gcaacgccaa tccccagaac gctttatatg tcgttatccg
    22081 gcataaaaga tatgagcgta atcaacactc cgcccaaaaa caggcttccg ataaaaactt
    22141 ttgtcggcga attcaatgaa agtcaggtta aaaacgccat tgtccatgaa ctggacagag
    22201 aaggtcaggt tttctatctt tacaacaggg tagaaaccat tgaagaattt aagcttcagc
    22261 ttcaaaaaat agtccccgag gcaaaaattc aaatagggca tgcgcaaatg gatgaaaaac
    22321 aattggaaaa agtcatggtg gattttgcaa acaaagaatt tgacattctt ctttgcacca
    22381 caataatcga atcagggctt gatattccaa atgccaacac aataattatt cacgattgcg
    22441 acaagttcgg actggctcag ctttatcagc ttagaggaag ggttggaaga tctgaaaggc
    22501 aagcttactg ctggtgtttc tacaaaaaaa gcaaggaatt aacccaagaa gcaatcaagc
    22561 ggcttaaggc gataaaagaa tttaccacgc ttggaagcgg ttatcagatt gccctgcggg
    22621 atatagaaat ccgtgggatt ggaaatattt tgggaacaaa gcagcatgga cacatggtta
    22681 atgtcggatt tgatacatac tgccagcttt tagaagagac tatcaatgag cttaaagggg
    22741 aaagcacaga aaaaattccg cctgctatag ttgatataaa cgtaaccgcc tacattcccg
    22801 acgagtgggt gggctcaaaa gagcaaaaaa tgattgaata taaaaggctt tcggacgtta
    22861 aatcccaaat cgaactcgat tatattaccc aagaatggca agacagattt tccaaagcac
    22921 ccgaatccgt gcaaaatctt atcaaactta ttaaaatcag gcttgcggca accgatgcaa
    22981 aaatttcttc gataagagaa actcacgaaa atataagaat ttataccccg tatcttttgc
    23041 ccgaatggaa cattattaaa ggacatttac ccccaaatat tacaaaatat ataaaattta
    23101 catctgtccc aaagtcttgt acagaaggca attccatcat tttgcttgac aattcctgta
    23161 tgaactttga tgaagtattt aacatattga ttgatttgtt ttattatatt tataaggtta
    23221 gtaaaggtta cacaagttaa gaggagcata ttatgaacac caaaaaatta tttgcaacta
    23281 ttgcaatctc tgccttgctt ttaacaggat gttctttcgg caaaaaagac gtcataataa
    23341 aagttaacga cagagaaatt acacaaaccc agtacgatca agcttttgat aaagctgcgg
    23401 gaaactctat gtttgctaaa atgggaatag atattaaaaa agacaaaaac ggctttttgt
    23461 atatgatgat taaagataga gtaatcaacg aacttgtggt gaaagcactc atagacggcg
    23521 aaatagaaaa aagaaatatc aaagttacaa aagaagatac tgataaagaa ttacaaaata
    23581 tcatagacaa aataggttca aaagaaaaat ttaacgaaat tcttaaacaa aacggaattt
    23641 cttcttcaca atttaaaaaa gacttaacag aagaagttaa aatgaaaaaa ctcgttaatt
    23701 cacttgccgt aataaaaatt tccgatgcag atgcaaaaaa attctacaga caaaatttaa
    23761 acaaatttaa atatcccgac aaagtaagag cgtcacacat tttaattgcc gcaaatccgg
    23821 aagaaatcaa agaaaaaata acttcagata aagctaataa aaacttaagc gaagaagaag
    23881 ttaaagcaaa aataaaacaa gaaatcaatt ctcagcacga agaagcaatg aaaattctta
    23941 ccgaagttaa aaaagatccc aaaagctttg aaagaatcgc aagagaaaaa tctcaagaca
    24001 caacaagcgc aaaacaaggc ggggatttag gcttctttgc aaaacaggaa atggttgaac
    24061 cttttgcaaa agcagctttt tcactcagac caaatactgt gagcgaaatt gtacaaactc
    24121 cttacggtta ccatatcatc ttagttacag acaggatgag tgcgggtcag gaaccttttg
    24181 aaaaagtcaa agatgaaatc aaaacctacc ttgaagccca agaacaggtt aaagtgcttg
    24241 aaaaccttat tgaatcactt aaaaaacagg caaaaatcga atatgtaaat cctgaatata
    24301 accctttgac tgttcaggat atattgaaaa aacaggcaaa atccaaccct gccgcaaaag
    24361 aagaaatgga acctcaaagc gcagcaccac cggctacgcc tgcaaaagaa taaatactct
    24421 taaaaagacg aggcgggcaa aacccgcctc gtcttttttg tgaataaatt aaaaaataaa
    24481 gcagctcata agccgggttc tgttcctaga taatcatctg tctgggacgg taattactta
    24541 ccgccacaag cgggtttcaa agaaggcggc ggacgacctt aatcttcttt tccttgcatc
    24601 cgatcggggt ttacctggcc gtatcctctc gaatacgccg gtgaagctct taactcaccg
    24661 ttgcaccatc gcctgtactg attgctcagc catcggcaga cttcatttct gtggcactat
    24721 ccacgcgctc acgcgctccg gggtttcccc ggcgatctgt ccttagatgc ccggactttc
    24781 ctcacttttt gcaaagcgcg attatccggc tgctttattt tgatatttta gcacgaaaaa
    24841 tttattataa ctcatcttgt ccggctttca cccataccgc cggctgtccc gcagccggta
    24901 tgggtttagc acttctaagg tcgaatttga aaaattaaga aaaattgtca tttttcaaat
    24961 ttttccacat cctctaaagc aaaaacattc cttcccacct ataatgggaa ggaaccttaa
    25021 tccattatta ttaaccgcat agaaagatta ttaattaaga gcgtaagtca gcgacatttt
    25081 aaaccaaacc cgctaaaaga aaaactctta tcgcaacccc gaaaaagtgc ttggatgttg
    25141 ccaacttact taattataat acaacttttc gcatgccttt tcaccattct ttagaatgac
    25201 atcaaatgta aaatataatg ctttagagtt aatactttat agttatgttt ttgtgtgtaa
    25261 ttttttattg aaaaaaaatt ttttttgtat tagactagag cctaaagcag tattgaaaat
    25321 taaatattac aaaaatatgc ggaagtaact caatgcccgt tgaatctttt acgaaagtaa
    25381 aagttcaaca aaagttaaat aaactctacc attgagttat ggtgaaaatt gaatattatc
    25441 aaaatatgcg gaagtaactc aatgcccgtt gaatctttta cgaaagtaaa agttcaacaa
    25501 aagttaaata aactctacca ttgagttatg gtgaaaattg aatattatca aaatatgcgg
    25561 aagtaactca atggtagagt acttgccttc caagcaagct gttgcgagtt cgagtctcgt
    25621 cttccgcttt tttaaaaaag cgaaagcaaa ttatgatttc acaatttttt tcaaaacatt
    25681 ttaatgaaat attagaactt tctattgttt ttataaccgt tttggtactt tttaaagcta
    25741 tagatatctt taacgcaaac gcaaaaaaaa gaatccttgc aaaacgtcag gattccacaa
    25801 ttttgggctt tatgcctttg attaacaaag tgcttaaatt tacaatttta tttttggcac
    25861 tggcaacgat tttgcaaagc catggctatt ctataacgtc cctaatcgcg ggtttcggaa
    25921 ttacaggact tgccgtaggc tttggggcac aatcaacaat ttcaaatgtg ttcggctcgt
    25981 tctcacttct tgccgacaaa gcctataaaa tcggagacta catagttata aatcaaaccg
    26041 tccacgacaa agcagtagaa ggcatcgtag aagacattaa ccttcgctcc acaaaaataa
    26101 gaacaacaga cggattaatc attgtaccaa acaacatagt tgcaaacggt gttgtcaaaa
    26161 atacatccat aaaagaaaaa gttattccaa atgaaaatta aaatcgttgc acttggcaaa
    26221 attaaagaaa aatttttaaa agaaggcatc gacgaatttt taaaaaggct tactccatac
    26281 gcaagcgttg aaataattga actttctccg cttgaaatta aagacgaaaa tcttattcaa
    26341 aaaacgctca aacaagaagg cgaaaaaatt ttagcccaaa ttaagcccga ttcttatgta
    26401 attactttgg aaatagacgg ggcgaatttt tcgtccgaaa aattcgccag aaaacttgaa
    26461 gaaattttta acatgggcat aagcgaaatc gtttttataa tcggctcatc ccatgggctt
    26521 tgccccgaag tttcaaccag agctaattta aagttaagtc tttctaaaat gacttttctg
    26581 catcaatttg caagacttat tttaattgag cagatttatc gcgccttcaa gattcaaaaa
    26641 gacgaaacgt atcacaaata aaacagttct tttattgaat acccttaatt ctgtcttcgg
    26701 gctctgtgat gcttgttatt ctcaaaccga aattatcttc gattacaacg acctcaccgt
    26761 gtgcgacaag ctttccattt gcataaagtt caacgggttc accggcaact ttttcaagtt
    26821 cgataattga gcctcttgtc aattccaaaa cttttcttat aggaagttct gttctaccca
    26881 gttcaactgt aagctgaagt ttaatatcca tcaaaatatc aagattttta ttgacttctc
    26941 cctgagaagg gtttatatct tcaaaagaag caaattttat aggccttacg gttacaggct
    27001 catctgtagc ctgaatttgc gaatcctcat tatcaactac ggtagattca gcaggctctc
    27061 tttttacaat aacttcttct gtcggttggg cgacaacctc ttctttctcc tctttcactt
    27121 caggtgcagg agcttcttgt tttatatctt cttgttcaat tttttcttca tcgggcataa
    27181 atttctcctt gattaaatat aacctttaag tgaatcgata gtaggatcat acctgtccat
    27241 aattttaaca cagacatggt ttttcaaagt tccgggtcgg gcgaaaaatt tcttttcatc
    27301 gttaacttta acaactaaat cttcgttagt tttgttgtcc attttaagaa tgtccccttc
    27361 tttaatgtca agaaaatctt caaggcttat ttgacatgaa ccgtacacca cgctcaaatc
    27421 gacttttgaa gtatttagtt tttgaatcat tttttgacgt tcttcggttg ttgctacaat
    27481 accttttgtc tggaatatat gctgggtact taaatgcccc aagacggttt ccaaaacagg
    27541 atatgggaaa cacaaactta taagtccgaa atattttccc gaaatttgta cttcaaaagt
    27601 aattagtgcg acaatttcgc ctgatgttgc aacctgaatc atctgataac tgttatcaag
    27661 tcccataagt tcagattgga caggaactat attgctccag gcactttcta aagttttaat
    27721 ggttctttca atgacttttt ttgctaaagc ttcttcgata tcggtcaatt ctctttgctt
    27781 attttcgctt aaacccaccc cgccaagcat cctgtcaacg atacaagaaa gcacctcaaa
    27841 gcttatgcct aaaagaattt gtccgggcag cggattcatt tcaaaaatcc ctatagtaat
    27901 aggtgatggc atagaacgca taaattcatc gtaagtaagc tgatcggttg atacaacatc
    27961 aatttcaata tgcatcctta aatatgccgt caaaacaaga gaaatctgcc ttgaaaactc
    28021 cctgtgaaca tcttgaagtg cccttaagtg atcttttgaa aatttatcag gacgccgaaa
    28081 attgtataat ttgtaacctt ttttatagtc ttcatcagca ttatctccgg agtaaggatt
    28141 ataaatatcc tcagccgatg attcagaatt tgttgtattt tcatctgcaa cagcaactgc
    28201 cgaatctgcc tccaaacccg ctgcggattt ttcttcagat ttagcttcag tttctgctga
    28261 ttgttctttt tcttccggag tttctggcat tgtacctcgc tactgaatta taaattgccc
    28321 gaagcttact cttatgactt ctctttcacc cgaaaagatt ccattgaccg cttcggctat
    28381 ttgttctttt gcaagttctt tgcctgcaac cgttgaaagc tctgccgatg ttttacttga
    28441 aagagttgtt ataatagcat ctctcataga aggtttaaat tgagccattt ctgcttccac
    28501 cggatttact gctgctgcac cttctccatg cccgccgcct tttgcagatt ctgcttcaag
    28561 ttccatatct tgaggagatt ttgagacttc aagtgcgaca tttactttaa gatactttct
    28621 aggtgaaacg tcactgagat ttaaaataaa atctcccaaa tctacaataa ttcctctttg
    28681 aacttcatct cctgcccctt cttcgccttc tgctgctgtt tcttctgtcg cagatgtttt
    28741 ttgagctaat tttgaattca ataaattagc ctgcagaata taatttacac ttaaaaacag
    28801 tatgcaaatt aaaacagtcg ttgcaagatt aatgataatc attttcatgt tttcatcaat
    28861 ttttattcca ccacccttag gtgacgcttc tgaagctcct tcttcacctt ctgcctttgg
    28921 tttaatatct tttggttgat tcggttttgc cattttattt tctccaactt aattactatc
    28981 ttttccaagc actattatat caactcttct attcaacatt ctaccactaa ttgatgtatt
    29041 gtcggctatc ggataatatt ccccatagcc taccgcacaa agtctttttg gcggtatctt
    29101 acttgttttt gtcaaatagg atattatgtt tgttgctctt gccgttgaca attcccagtt
    29161 tgaagggaat ttagcatttt ttatcggaac agagtctgtg tgcccctcta ttataacagg
    29221 gttgtcgatt tttgttaaga cttttataat ttcacctaac gcctttttcg accgaggttt
    29281 tatatctgcc gaaccttcat caaaaagcat tttatcgctg accctcacaa tcaagcccct
    29341 atcggctttt atcaatttta ttgattttgt ctgcgaaagg ttttgctcaa ataacatttt
    29401 taaactctgg gttgaataac ttttatcacc ttctgtaatt ttgtgatatt tcgtaacatt
    29461 ttcaatttga ggagtatctg aaaatgtttt ttgaagtttt gcctgaagtt ccattaattt
    29521 ttggttatca agctgggaaa ttgcaaacat taccataaaa agtgccaaaa gcattgtcac
    29581 aaaatccgca taggatacaa cccagcggtt tatgtaatct tttgaatttg tgtagtagtc
    29641 gtttctcatc tcagtgccat atgtttttta ttatgaaatc ttaaatatgc tacaagtttt
    29701 tcttcaatta ttgcaggatt ttcctgcatt tgaattgaaa taatcccctg caggataact
    29761 tcttttaaaa gaattttttc tcttgttttt tgttttaagt ttcctgcaat aggaagcaaa
    29821 atcaaatttg caaaccccac accgtaaagc gttgccacaa aggctgtcgc aattccggtt
    29881 cctaaaatgt gaagatcttt gatatttgac ataacctgaa ttaagcccat aacagcaccg
    29941 acaatcccga aagtcggagc atatccgccc agtgcctcaa aaactcttga gtttatcaat
    30001 tcttcttctt cttcgtagga aatctcggac gacaacaaat catataaaag ctgcggatta
    30061 tttaaatcaa tcgcaagctg aattccgcgc gataaaaaag gatcttgaac tttgtccaaa
    30121 actccctgca aggataaaag tccgtttttt cttgcaaaat aagccagctg aattatatcg
    30181 tcaaccgctt tcatggtttt gttttcctga tggtaaaaaa tttcttttgc gtgcctgaat
    30241 gcggttttta acgtctgcat attaaaattt aaaagcgaag cgcagaaagt tcccccgacg
    30301 acaatacaaa acgcggtcgg ctgaaaaagt accgaaattg aacccccttc aaatatttgg
    30361 gtgataaata ccattacaaa acctaatgca agtgcaaaaa cagcagttaa attcaatttc
    30421 cttctcccta tttctcccta cttctcccaa tctgacaata tcatcgcata ttttaaaggg
    30481 cttagccatc atccaggaaa agtatttttg ggtaaagggc ttaataattt taacttttaa
    30541 gccccctata cttattctta tcatttgtac tatcttgaaa aacattaaaa aatatgtgat
    30601 aatataattg ctttacacgg gtgttaattt tgttccttca atttttataa acgggagagt
    30661 tgactctgaa agtattgaag taaacccgcc tttgaattaa cttcaaggca agttcatttt
    30721 ttgaactgac agcgggaaac ggattaaata aggcactcac ccacctgcga gattaagcag
    30781 gtaacaaaat tcgctcgtgt aaagcattat ttcagcgggg caaaaatctt tgatttttgc
    30841 cccgctgaat ctataaactt attttaattc attttcaata aaagtgacaa actctttaac
    30901 caaatcttgg ttccattttt tgtcagaatc ttttttaatt acctcaagtg catcttgagg
    30961 actcatcgcc tgcctatagg ctcttggttc tatcaaagca aaataagcgt ccgttattaa
    31021 aattatttga gaagaaagcg gtattttttc gcctgaaaat ttaccgggat atcctgttcc
    31081 gtcccagttt tcatggtggt gttcaattat cggaataaca tcttgaatat ctgaaatcgg
    31141 cttaagaatt tctcttgctg caatcagagg atgtttttta atttcagcct gctcttcttc
    31201 tgtaagcggc tctatctttt gcagaatctc ctgaggaagc ataatatttc ctatatcata
    31261 aagcaatgta gcaattctaa gtttgtcaat ttcttcttta ggaaagtcaa aactttttgc
    31321 caaatttgaa accataagaa tttttgactt ggtttttccc ttggcgtgta taggattata
    31381 agtagcagaa agcatgtccg caacagaata aagcatctct tttggagtgg agcttttttc
    31441 aagagaagtg gcaagttttt catgcaattc tgaggcaaca tcttttggaa tgggaagcct
    31501 ttttcttgaa agtatgtcca taaaggtcgt aattgcaact tcctgccatg aagtttcact
    31561 gattggtttt gcaacttgaa cgcgatttct gccgtttctt tttgccatat acattgcctg
    31621 atcggtaatt tcaagaagct catcaatttt atcggagtgt tcgaggaaag ttgccaaacc
    31681 tacacttgcg gtgattttgc cgacttttct gattttgcaa tcggaaattg cttttctgat
    31741 tctttctgct gcgataagcc cgccttttga atcgacaccc ggaagaatta tgttaaattc
    31801 ttctccgccc attcttgctg caacgtctat tgagcgtgca tttttcttca aaacttcggc
    31861 aacttcttta attgccatat ccccataaga gtgcccgtac ttgtcgttaa tttgttttaa
    31921 gtagtctaaa tcaaggctta taatagtaaa cggctggttc atccgctctg cccttgtggc
    31981 ttctttttta agaaattctt caaaatgtct cctgttataa agccccgtaa gagcatctgt
    32041 tacagcctga tttttaaccg cctgaaacaa atcggcaatc gttattgcaa gttcaatttg
    32101 ttttgaaaaa agatttaaaa aatcaagttc agcttcactg gcttcttctc ttgaagaaaa
    32161 aactataagc caaccgaatt cagtatttcg agtccaaagc ggaacaagaa ttatagaacg
    32221 gctctttgca ttatttaaaa tttttcccac actgtcttcg gacaaatctt ttgaaacagc
    32281 tttaattata cttctcaaat ccgttgtttg aacaattcgc ctattggtta atgcttctgc
    32341 aaacagactg tcctcacaat aatcaaattt tatcgtctga ataggtactc caaatgccaa
    32401 atttaacctt tcaataagct cgtcttcggc ataagttatt gttttaatgt aattgccctt
    32461 ttcgtcggtt tttttctgca taattccgct gtaaaggtaa cctaaatcac cttttatgct
    32521 ttttgttatt gttgccaaaa cattagaaag cggagtggaa gaattcatca tctcccaaat
    32581 atgctgcaaa gtaagcatgc gctgattagc tatatcaagc tcttttgttc tttccgatat
    32641 ttctttttca agttgaagat ttctttcata aatttcaaga aaatcttgct tatttgcata
    32701 tattctgttt tcaacttccg aaatctgtcc ggtaaactct tttatcttat ctaaaaaacc
    32761 catttaaccc cttgtctgtg gttgctaacc cactttatta tacaacagtt taaaaaaatt
    32821 gtaagggtta ttttaataat tgatttacaa tagttattat tgtatttggc acacaaatat
    32881 tcttaaatta tctttacaaa ctagcaaaaa caatttttat gagttttatt acactaaatt
    32941 gttattaaac agttaggatt ttatagaaaa gggaaataaa tgttaagaat aagttcaaca
    33001 atgccaatct atacggcgaa tgttacctct aaaaactgta taaaatcagt aactttcaag
    33061 ggcaaacaag tgccggaaga aatcgtaatt cgcccgggac aagaaattcc agaaattatt
    33121 aaagcaaaaa aagtaataat aaaaggcgaa taccaccact ggatagtcgc taaagacaca
    33181 aaaataatct gcgaaacatg tgaacttgaa catgccatgt ttaaagaaat aaatgcttca
    33241 aagtctgcaa cattatcgaa agagtccaga gttaacgttt taaataccaa tgattgggcc
    33301 tctatttcac tttcaagcgt tggaaaaata aatgcaaaag gcagggtttg tatgcaatgg
    33361 atggctagtg cagatgaggt aaatgccggt gaaattttga tgataaattc tcgggttaaa
    33421 agtttaacaa cagaaaaaac ggccgattta agcgattcta aggtaaaaat cctcacctca
    33481 ccggctgtta aaatgagtaa tcaatctgaa gtggaaaacc tgagtgcaga ttatctacat
    33541 gcagaagatt caacggttaa atcaggcgtt gtcaaaaaat caatttataa atgcaacagt
    33601 gcaattaatg taaagtgcaa tgatatctat gacgacacat tttcaaaatc tttgcgtgaa
    33661 ttacaggcaa gtgaatgcaa ccgttagtag tataaaaatt aaagcaattt atttactacg
    33721 cttataattt cttcacttga aggatatttt gtgcattcat ttttgcccgc tttatgtctg
    33781 catcttttct tgaaacaagg ctgacaatta agtttattgc ccaatactga aaaatatttt
    33841 ccttcttgtc cgtaaggacc gaatcttttt ggcggagtac aggtaaaaat tgcaataaca
    33901 gcaggaattt gtgttgccca tgccagatga gcactccccg aatcagggct tacaacaatt
    33961 tttgagcgcg aaaacaattc gattaattgt tcaacatttg tttttccgac caaattcaaa
    34021 tactgcggag ttgtttttgt tttgcccaaa atcctttgta ttaagtcctc gttgaagtct
    34081 gttcctgtaa aaacaatatt gcatttgccc tgtatttcat caattaaatt tgcccaattt
    34141 ttttcatccc aatgcttatt tttccaggtc gtagcaggcg aaataacgac aatcggtttt
    34201 gaatggtcaa tttgtgccaa taattcgctg actttgtttt ttacatcaga acttgtcgaa
    34261 ggaagcgcaa aatttattcc ttttggctca atccctaaat gtcttgcaaa atcaagattt
    34321 ctttctacta tatgataatt taaatcacta aactttgcct taggctcaac aaattcgttt
    34381 gcacccaaaa aagacaattc acgggcatct ttaaaggtta cccgcctttt tgccccgcaa
    34441 aacaacaaca aaaaaagact tttgaacatt tgctggcaat caagtgctat atcgtatttt
    34501 tcttttctta tctgttttat tatttgaaaa aattccataa aagttttaaa agaaaacggc
    34561 ccttttttcc actcttttag aggcacaaag tgaactttat cgattttcgg gttatttttt
    34621 ataaccccta accctttttc tgccaccacc cagccaactg tataattttc atcctttaaa
    34681 gtacaagcta aaggcaaagt aaaaataaca tccccaagag aagtcaatct tattattaaa
    34741 acttttttct gattatcact cactgctcac tgctcactgc tcactgcatt ataacatgac
    34801 tttcaaaaga atttcattaa attaaaaata acaggtcata cgaaagtatg attttcacca
    34861 aaaaagttgt gcaaaataaa aggttttacc gacaaaaaac acagaagact tcggttaaaa
    34921 agggcttaaa atggcttaca acacaataac tccgttaagg caaaagcttt atacggtgca
    34981 taacaataat tttgtcaaac ctaaggaaga tgaagaatct tcccacctgc cgcagcctgc
    35041 cgaagaacag acagaagcag aacaaagccc gaaacttacg cttgacgaac aaaaaagagt
    35101 ttttcccgag caagcggaaa aaaatcaggc ttcaataaac tcgcttaaaa acgccgttaa
    35161 catagcacaa atcttaaaag atttcaggag caccgcttta gcaataggca ctccgcctga
    35221 tttaaaccag gaagtggaaa cctacctttt attggttgac acacaggtaa gaaaagaaaa
    35281 tcctaatata aaaacaatta agtcaaacct taaaaatgcc tctgcgcttt tagacggata
    35341 cataacagaa acccttcaaa gaccttcaaa tgttgttgaa aactggcttg atgcattatt
    35401 tttacaacaa gttgatttta aattcaacga aaacgacgtc aatcctcaat ttcttgtcca
    35461 attccccgat tctgttaaaa cttctcaaac agaaaatacg caacaaaccg aagaaacttc
    35521 ccaaactgaa gaaaaaacaa atcaaagcgt tattattcct caagacgccc aattaaaatc
    35581 gctttttatt caagcaaaaa aattggctta cgcaggagag gctaaaaggg cacttgaaac
    35641 ctttgaacag gcaagaatcc gtgcaattga agttaaagac agcgaaacag aatctaaagt
    35701 tttatacgaa atcgggcaaa tttatgacag caaagatttt cttgcccaag ctcttaccag
    35761 ctatcataag tctttgggtg ccgccactga tttaaatata aaaaccaaag cccacttctc
    35821 aatggcaaaa atctacgacg atgttgccca atttgaaccc gcaatcaatc attacatgag
    35881 ctcaatttcc tttgccggac aaaatgataa ttttgcagcc caatcaacat ctctgactaa
    35941 aatcggaaat atttttaccg acaaatatga caaaaaagct tttgaatttt acgacgaagc
    36001 aaaaacaatt gcagatgaga gcaaaacccc aaaaattaaa ggctacgttt caggcaacac
    36061 tgcaggtgct tatgccaaat ttaacaatcc taaacttgcg cttcaaaact actctgaggc
    36121 agtacaagaa tatcaaaatg caaaatctcc cgaacaagct gcgattaatt acaaaaaagc
    36181 cgcacaatta atgataaaac ttcaaaattt caacaaagca aaaagtcttc ttcaaaaagc
    36241 ccttgcaaat gcaaaacaaa caaataatac agaattgatt tcaaaaatta attctcaact
    36301 ccaaacccta tagaaaaaag ggcgggcaaa atttattttg cccgccctta aatcttaaca
    36361 gtaaactaac ctctgatatt aagttttttg actaattttc tgtattcttc aaggttttcg
    36421 cctttcaaat aagaaagtag tctttttctt cttcctacca tcattaaaag tccgcgtttt
    36481 gtcgcaaagt cttttttgtt aatttttaaa tgttcggtaa gttcgctgat tttttggctc
    36541 atcatcgcaa tttgaactga agcagaacct gtatcttttt cgtttttacc gtatttttta
    36601 acaacttctt gtttgttttt aagattaatg ctcatgtaaa atattccttt ttcttagtaa
    36661 tagcaatcgg cgtaagctat ttatacgaat taatcatatt acataaagaa aaaaaatcaa
    36721 tatttgccca aagttatgtt acaagcactt aaaaattaac ccaaaataga attggcaata
    36781 aatgccgatt cattcatttt tgagcaaaat gaaatggaac ttcttaagct atcgcgaaga
    36841 atatcagaat cggattctgc ttcaaacaaa cttttttgag cgttttggta tttagtttta
    36901 aattcttctt ctttgcttgt gtttttataa gattgaaaaa ttgtaaaagc tttttctttt
    36961 aattcgctaa aaataccgta ttttttcatt gaagcttcat aaatagtccg tttttcgtac
    37021 attttttgac gggcatcttc tgcttgatta ttaatacttt ctaaagttct ttgctttaag
    37081 tcgtttgtga caaaatccat cccaaaatcc tcattaatat cccttatata tataaaaaca
    37141 aatttcataa aaaaattgcc ttttttcatg ttaaaattaa ttcggaaaaa ggatttttta
    37201 tgattgaaag atattcatta aaagaaatga aaaaaatttg ggatttacaa tccaagttcg
    37261 gctactattt acaggtggaa ctcgctgttt gtgaggctta cagtaaaatg ggacaaatca
    37321 gcgcaaaaga tttggacaaa ataaaaaaac tggcaaaatt tgatttaaaa agaattgatg
    37381 aaattgaaaa ggaagtcaat catgacgtaa ttgccttcat aacaaacatt aacgaaagtt
    37441 taggaaattt atcaaaatat tttcacctcg ggcttacaag ttccgatgtt atagatacgg
    37501 cacttgcgct tcagataaaa gatgccgcca aaatcataaa caaagacctc gataatgtta
    37561 tttcttcttt aaaaaaactt attaaaaaat gcaaaaaaac tgtctgtata ggtcgctcac
    37621 acggagttca cgctgagatt atgacttttg gggtaaaact atgcagctgg cttgatgctt
    37681 ttgaaagagt aaaagaaaac tttaactttg cacttgaaaa aataatggtt gggcaaattt
    37741 caggaccggt aggaacttac agcaacattt cgcctgaaat tgaaaaaatt acctgcaaaa
    37801 ttttagggct taagcctgca aaaatttcaa cccaaataat cgcaagagat atccacgcac
    37861 aatttatgca atcattggca ttgattgcaa gtgttataga gcgttttgca acagaaataa
    37921 gacaccttca aagaacagaa gttttggaag tcgaagaagg gtttaaaaaa ggccaaaaag
    37981 gttcttctgc aatgcctcat aagaaaaatc caattacaag cgaaaacctg tgcggacttg
    38041 caagggttgt aaggtcaaat tctattgctg cacttgaaaa tatagcactt tggcacgaaa
    38101 gggacatttc gcacagttcg gctgagcgca taattttccc cgacagcaca attttagttg
    38161 attttatgct ggaaagattt aaaaatattt tggataatct ggtcatacac gaagaaaata
    38221 tgctcaaaaa cacaaacctg tttggcggaa taatttattc gcaaaaagtt atgctggaac
    38281 ttgtaaacag agggctttca agagaaaaag catacgaaat tgtgcaaaaa aatgcccttg
    38341 atgcttttga aaaaagtggg aattttaaat caaacctttt aaaaaataaa gaaattaaaa
    38401 aatatctgtc tcaaaaagaa attgaagcct gttttaatgc acaggattac ctgaaaaata
    38461 cagatgaaat ttgctcaaaa tttgattaac taccaatctt tggcaccgta ttttgaataa
    38521 atttctataa tatcatttat tttttctaaa atttttaaga tattttctct ttctgattcg
    38581 ggcaaatctc ctttgagtat aatttcatag gataaaatat cttccttgag ttttcttata
    38641 tactgttttt tttcttctct ttttttaaaa agccacccct cgtagccaca ccccggaggg
    38701 actactaccc aagggtttga agggaacctt ttgcacaact cgggtctttt ttcataaatt
    38761 ccgcacaaat tgtcatcttt gatataacga catcgataaa aagtagcctc tttttcatca
    38821 aaatcttctt tttcggagtt gagcttagaa agtatgtttt caaccgtttt ttgacaaaca
    38881 ttttttgcat cttgtattga ttgataaggc tcaaaaatat ttaaaaaatc tttggcatat
    38941 tcatccccct gccttacaag actttttaac tcttgaaagg acagtgaaga agttgcaacc
    39001 ctgcaacatt ttccacacat attgcaaagt tcctgcggac ggttttcgat aaaattttga
    39061 gttttttcca tgtttcctca aaattattat atcaaaaaat taacttaaca tttcttaaaa
    39121 aatttaccga aagggcaatt atttcatggt tttagacttt atttatataa gtggtgaaag
    39181 gtaaggtttc atgcaaaata cagttgtaaa ccctcaaact gtagctcccg cacaagctgt
    39241 ggttactcct gctgctggta atcagcctca ggtagctact caacctgtgg taaaccccgg
    39301 agttgtagca acacaaaacc ctcaaacagt tcaggttcct aattactctg gggttaatat
    39361 tcaaatatac aacccttctg taatgacacc gggaacaaac atggcttatc cttcaaatta
    39421 ctatacaaca aattacggtc ctcaaggtca gcctcaacaa gctgcagcac ctgcggctcc
    39481 tgcagaaggg gacaaaaaga aaacagaaaa acgcgatatt gtcgaattga cagatgatta
    39541 tattaaaaat cttgaaaatt acctgaacag tcaagaccct gaagtcagaa tgatgggcgc
    39601 aaaagaagtt cttgcaagac ttcaagaaga cgacagcaga aaagacgaca aagctttaaa
    39661 tgccctagta aacaaaatgc tccaagaccc ttcacaaaaa gtaagagtta tagctcttgc
    39721 agcacttgat tcaaaagcgg taacaggtga tgatttgacg gtacaaattc tcaaaaacat
    39781 gcaaaataaa aagacctccg acgggcaaga cgcaatgcag gcttcccaaa tactgttaaa
    39841 aatgtcggga aatactgtta aaaaagaatt tgaagtaaaa gataaaccta aaaaagaaga
    39901 aaaatccgat aaaaaagaat aaggaatttt aaaaatgaat atttcaaaca tagcaataaa
    39961 agcaatagga atagccggct taggcgcggt tttatacgat tctcatacaa tcggaaaaga
    40021 agaatgcaat atgaatcaac gcaatcttaa aaccgacagc gtagaaaaaa cttttatgga
    40081 cacgatatct cttgatacac ccagcaaaac caaagatatt atgaaaaaac agtttttaaa
    40141 atacaaatta aatgaaaact tcacaacatt cttcacagga gctgcaggat atgtaaaagg
    40201 atttactaaa atgttggtaa ataacgtaat tccttttggg ttggcactag gaacggttgc
    40261 cacaaaagga ctcgcctcca aagcatgcgg tgtgggttta atcggctacg ggctggtgta
    40321 tttgctgcac gatgtaatgg gaatatttaa acccaaaaag ttataaaaaa gtttgataat
    40381 aatcaaaagg acttgttaaa aacaagtcct tttttataca catcgcgggg taacgattta
    40441 taagccttaa attttttatt ctgtcatttt aagagcaact tctcttgccg caggctctga
    40501 caagcctaac tctttttgaa cagctgccat attttttcct acttcatcag caaatttatc
    40561 catcatggtt gtcattctag ctaccacttc cggagaagca ctctcagcca ttgcaaaagc
    40621 ttttttcaat gaagagctcg attcaattcc caatacagct tttgcaccca taaaccctaa
    40681 aacagcctca ggagaaacct cttgacgttt tgctgaacct tcagattctt tttctttttc
    40741 aacttttttt tcaggagctt ttcctgcccc acccattttc gcacctacag caaaactatt
    40801 taaccctatt ctatcgattg acattttgat ttctccttag atttcgtatc cactttttgt
    40861 ttatcggttt aatttttaat gacctttaga aaaaagtctt tttttgtaac aactttttac
    40921 aaattgtaat aatttgaatt attttgtaaa ccttttgtaa aaaatatggt aaattaagca
    40981 attatacccc tttacatgtt ttatagactt gggtaagaat aaaatgccga taaaaaaaat
    41041 taatagtgta aatactgatg cgccttcttt tggcgcactg cctaagccct ttgtaattaa
    41101 aagggcaaaa actattgaca tacttgaaaa aatgggcaaa ttttcttctc cccagcagcg
    41161 attatttttg ggcgtaacgg cattaatgac ccaacctttt atagacctta acaacaaaag
    41221 cgtggatgaa aaaactagag tggtttcttg tgcaagaaca attgcaaaaa actttatagg
    41281 aacaatcgtc ggcataacag taagagagct ttgtattaaa gcgattgatt tttgcaccga
    41341 agtcgatgct aaaaaattaa aagatacaaa atattttaaa ttcaagacag ccttgctccc
    41401 aagcaatgtc aaattcaagg aagttacaag agaattggtt aagcacaggc aagctcttgg
    41461 tacttttata gctcttggag caatgctttt tacaaacttt gccattgacc ttccgcttac
    41521 aaaatggatg acaaacctgc ttgtcaaaaa aattgaaaaa ggcgcgcaga aaaaactgga
    41581 aaaacaaaaa caagataaac tgcaaggagg gggtaaatga ttatgcttaa atctttaccg
    41641 ctcatatttt tgcaaggagg gagcaaatga aattatcctt gcaaaaaaca gtagatgggg
    41701 tttacaaaca tttttcccat gatttgggat ttatgctttt ggtcacaggt gctgtgggct
    41761 gggctctttc ttcacttgcc catgttactg cagttgttat aaataaagac attccaaaag
    41821 aacaaaaaaa ctttttgatt cctcaagaaa ttgcagacgc ttttgttaat atcgcagcct
    41881 ttattgccat aactcaccaa ttcaataaat tcggaacccg tatggttgaa tcaggcaagc
    41941 ttactaccgc gcccattcgt aattttttga ttaaaaatca agaatctttt aaaaacctga
    42001 atgaaagaat tattgattca aatgatttga tttcaaaata ttttaaaaag gtctttaaaa
    42061 atgattctgc cgccaaacaa aaacttgaac ctttctttac taaaagaaaa gttaatataa
    42121 cagattctga tgtatttaaa aacaaccaag acaatatcca aaaagatttt tattcgtttt
    42181 atgacggagt tgattttata tcaagcacag taggtgctat agtttcgtgc aacattgtta
    42241 cccctattct tagaaatata tttggtgcaa aacgccaaaa atctcttata atgcacgata
    42301 aacttcacca aagtagcatt atgtttgcaa aaccgagggt tacgctggaa agttatgcac
    42361 aaaacgtaag ccaaaaaaca agcttaacct cgccaaattc ttctatgaaa atctaatcta
    42421 caagaccttc cctgactgct tttacggcag cctgaaccct gtcttccaca gacattttgt
    42481 gaagaataga gcacacatgc gcctttattg tatgcgaact tatggctaat tcttttgcta
    42541 tttcggtatt actttttccc agggtaacca aattcaggac ttcaaattct cttttagtaa
    42601 gattttttga aaagttttgg ttttcttcca ttaatacctc aaaggttttt gtaaaaaaat
    42661 taaaaagcgg actgacaaca ttataatacc gtcagtccgc aaaagtctat ttattaagtt
    42721 ttattacctg aataagcctg ctattcgcaa ctgcctgttg ttggattgta tgtcattggc
    42781 ggcgtaaggc tttggcaata gttattgctg gcgttatcgc taacacccga aacggtattt
    42841 cctgctttat tccccgcctc ggtaatcgtt ccgccaagtt ttgtaagcac agctattgca
    42901 actattgcaa ccagtgccaa aatcaatccg tattcaacta aggcctgtgc agccttttta
    42961 attattattt tttttgtttt tttcattatt taaattttgc tcaatttata gactgttatg
    43021 gggttatgtc acaaaatttt gtgtcctcat tccattgtcc actaacagag ttacaataag
    43081 ctgctcctga attctctgta actttttgta cttgagtatc tgtgctgttt ccaacgttgc
    43141 cgattgttcc gccaaactta ctcaaaacgg caagtgcaac tatcgcaaca agtgctaaaa
    43201 tcaaaccgta ttcaatcaat gattgtgctg tctttttcat aaaactctcc ttgttattgt
    43261 taattatata attacaatcg gattatatca cttttatttt tataatgcaa atggtttttt
    43321 gggtgaatgg cttaacagtt gagaagtcga acagtctaat agttgaaaag tttttattca
    43381 cttctattta ccatttacta tttactgtta accccacttg caaaaaaatg cgaaatggtt
    43441 tagattatac atgactatga ttaagttgga aaatgtaaga aagagttaca gaacaacacg
    43501 catccttaat aacgtttcat tctgcgcgca tcgcggagaa attgtcgcta ttatcggtcc
    43561 ttcgggctgc ggcaaaacaa cattgctcaa atgtatagac ggactggaaa aaatcgacaa
    43621 cggaacaatt acaatagaag gtcatgacat aacaaaaaaa acaaaagatt tatcaaaatt
    43681 aagacaaaat gtcggaatcg tttttcaaca gtacaattta tttccgcaca tgacggtaaa
    43741 acaaaacata gtccttgccc cattgattgt taaaaggatg aaaaaaaacg acgctgaaat
    43801 tttagccatg ctgcttttag aaaaagtagg cttgcttgat aaaatggacc attacccgca
    43861 tcagctctca ggcggtcaag cacaaagggt tgcgatagca agaacccttg caatgaaacc
    43921 caaagtagtt atgtttgacg aaccgacaag cgcgcttgac cctcacatga aagttgaagt
    43981 tcttgaagtc ataagaaagc ttaaaaatca ggatatgatt attcttctgg taactcacga
    44041 acttaaattc gcaaaagaaa tcgccgacag ggtagtattt ttatacaacg gacaggtaat
    44101 agaaaatgat tctgcgcaaa atgtttttaa ttcgcccaaa aatgaggtta caaaaaaata
    44161 ttttgaaaaa atttctgggg cagaatagaa agttttaata tgaaaagaca agttatctat
    44221 atttttttat taggtttggt tatttttttc tcgggctgca aaaaacaaga cgaaacaagc
    44281 gatgttttgg ataaagtgtt aagccgcgga aaattaattg tcggagtaaa atttgacaca
    44341 aaacctttcg gctacattga cgagaaacaa aatttgacgg gctttgacgt cgatttggca
    44401 aaacatatcg caaaaaatct tttaggcagt gaagataaaa tagaattcaa gcaggtaacc
    44461 ccttcaaaca gaattttaac tttaaattcc ggtcaggttg atatgattat agcaacaatg
    44521 actataacag atgaaagaaa agaagtcata gatttttcaa aaccttatta tatggcagga
    44581 caggcaattt tagttcccgc aaaaagcgac attggttcta tggctgactt aaacggaaag
    44641 cgtgtcataa taattttcgg ctctacggca gaaaaaaata caagacttat ggcacccgag
    44701 gctaaaattt caggttacaa aacttatacc aacggctaca gcgcgctaaa acaaggcaga
    44761 gcagatgcaa tcacctcgga tgataccatt ttaatgggct ttgccatgca agatgattcg
    44821 gttaaacttt tgcccaaaag atattcaaaa gagccttatg caataggatt taaaaagggc
    44881 gaaagctcaa caagattaag acaaagagtg gatttaattc ttgacgaaat gattaaatcc
    44941 ggtcaactaa acaaattaaa agcaaaatgg attaaatact gaacttaaga cagttcccgc
    45001 aacaaatgcg ggatgacagt atgcaaagga ttaaatactg atgtatgaaa tggatttttc
    45061 aacactctta aaaaactatc cgatgataat cgaaggtatt ttaactacga tttcggtatc
    45121 actgcttgca ggtattttgg cttttgtctt aggaagtttt tttgcttact tcagaacaag
    45181 tcctttaaat attatttcaa aaatcacaag ctttatcgta ctttttataa gaaatatgcc
    45241 gcttttgata ctgatttatc ttttttataa gggtttaccg tcaattcatc ttgttttgtc
    45301 ggcatacgct tgcggaattt tagcattagg gctttacacg ggctgttata tttcagatgc
    45361 tataatctcg ggtataaatg cagttccgaa agaacatttt caagcaagcc aatcacttgg
    45421 tatgagcagg attaaatctt ttatttatat catttaccct caggcaacaa ggtatgtgat
    45481 tccgattttg ggaaaccagt ttatgagttt ggttaaaaac tcttcactgg tgagttttat
    45541 tgccgtaaca gacatatttt atgtcgttta caaagggatt tcagatactt acagaatata
    45601 tgaatatttt attcttgccg ttgttttata tctgtttttg acaggttttg tgcttttgtt
    45661 gacaaaaata ttgcaaaaga tttatagaat accctcgcag gaggtgattg tatgacatcc
    45721 gatgtaatca taaacaacct gcctttttta atgaaaggac ttttggttac gctgaaagtt
    45781 tcctttatct gcataatttt aagtttattt tcaggaataa ttttaggaac actgaggttt
    45841 gcaaaaattc ctttaataag ctttattgca ggacttttta ttgatttgac aagagcaatt
    45901 cctttgattt tgtttataat atttgttcac tttagtgttt ctccttattt gttcggatac
    45961 agtgcttttt taaattatat gggcatagat tcactggaaa tgtactctgc aataatcgcc
    46021 cttgtaattt ttaattgcgc atacatagca gagattataa gaagcgggct tgaaagtgtc
    46081 ggaaaagacc aaattcttgc ggcacaatct cttgccctga gcaaagttca ggtaattgag
    46141 cacatagtgt taccccaggc attgagaaga attaaaccct cgcttgtggc gcagttggta
    46201 acacttgtaa aagatacgtc tttagcatcg gcaatcgact taatagaatt aaccagagca
    46261 tgcgaaatag tttatgaaac ctcctatcac gaatttgaag tattatgttt cgcggcttgt
    46321 gtttatgtga gcgtaaattt tttaatacaa aaaatgttta atacggatat aaaatgttaa
    46381 actattgact ttttttttaa agtttgttag tatttatctg tactttataa tataaggtga
    46441 gttgtaagga ttttattgta tattaactaa gcgaaaggta gaaagattat gaaaaaaaga
    46501 ttaggattta ctttggcaga ggtattaatc actttgggta ttataggtgt agttgctgcc
    46561 atgactattc cgactttgat gaaccaaaca ggacaagctg agttcaaaac agggcttaaa
    46621 aaaatgattt caggaattaa tcaagccgtt actatgaacg ttgcactaga cagcgtaagc
    46681 ttttccgaag gagatgaaac caacaactta tataaattat tctctgacag aatgaatgtt
    46741 atcagttcaa catctgcagc cgttggaacc ggagatataa atgttgacga aggtaattat
    46801 atcatattct taaatgacgg ggcggctatt tcttggacag atactgttaa cgaaactgct
    46861 tgtacaacaa ctgtaggaac aaatgaagca acaggtgtat gtaacgtcct tattgacgta
    46921 aacggcaaga aaagacctaa tgcacttaca acttcatcaa ctgatattaa agaccagttt
    46981 ggtgtcagaa tcggtgggca acaagcactt ccgggtggcg ttgcaaaagc agttatgtat
    47041 aacagataat ttaatatata aactataaat ttaaaggcaa cagatatctg ttgccttttt
    47101 tgtgttaaaa ttgttatatg aaaatatcgg gagaaaaaca gttagattta ttgaccaatt
    47161 tggtaaattt tatatttagc attcaaaggc atttaacatt tatcaaggaa gttaataacc
    47221 ctgagcttaa gccttgcatt tacgccctat ggcacggaca tcagtgttgt attttcgggg
    47281 ttgaagacaa agctcatttg aatgtattaa taagccgctc gattgacggt gaaattattg
    47341 caagagcaac agaaaaatta ggattcaaaa cagtcagagg ttcaaaaggc aaaaaaggca
    47401 gcgttgaagc cacaatgcaa ttacttgaca ggcttaaaaa tagcgagtgc attgcaatga
    47461 cagttgacgg accaagcggt cctgcttttc aagtcaaaga aggggtaatt aaaatagcaa
    47521 aaatgtcggg tgctcccata attcccgttt attggtattc tcctcaaatt aattttataa
    47581 aattcccctc ctgggataaa ttgttgtatc cgcttggcgc tacatggctt gttaatttat
    47641 acggggagcc gatttatgta aattcggata acacagatga gcaagatgaa gaggcaagat
    47701 taaaacttca aaatgcattg aaagagcttg aagaaaaagc cccgcaagta cataaagaaa
    47761 tttataaatt cagattatgg aaaaaaaatt aaaaatagcg gcaattcaaa tgtcgtcaaa
    47821 aatcggcgat attgatttta acattaaaaa aattgatgga ttaatcaaaa aaaacatcaa
    47881 ggaagagatt gatttgctta ttttgccaga agtttggaca gtaggctgga tgcctgcagg
    47941 atttaaaaac tccgcaaaag atattgaaaa aagcaaagct tttttgtttt tatcaaaaat
    48001 tgcaaaaaaa tacaatacaa atataatcgg cggaagtttt ataacaaaaa gggattcaaa
    48061 atattacaat tcatgtcctg tgataaataa taaaggcgaa cttattgcaa cttacgacaa
    48121 aatgcacctt ttttcttact acggctgcga cgaaggagct tttgtagaaa aaggaaacaa
    48181 tcccgtaatg gttgaatttt gtttaaactc aaaaaagatt gcttcggtga acgataattg
    48241 taaaagccga caatctacgt ctcgcaatga cattgtcaaa atcggcttaa gcatctgcta
    48301 tgacataaga tttcctgaaa tttacagggc ttacgctaat gcaggggcgg atttattgat
    48361 aaacatggcc gcttggccaa aatcaagggc aggacattgg gaaacgctta ccaaggcgcg
    48421 cgctgttgaa aatcagtgtt ttatggttgc tttgacacaa tcgggcaaaa tcaccgaaga
    48481 tgaatggaat ttgggacatt caagaattat agatttcaac ggagaggtaa tttcacaaat
    48541 aaaaaagggc gaaggggtaa tttttgcaca acccgatttt gaaaagatgt atcaatttag
    48601 aaaaaaatgt acagttttac ttgatataca agaaaattat gatgtaaaat tgttttaagg
    48661 acgtcatgaa aaaaattaaa ttaatttcaa cttttatagc attatttgtt tttgcacaga
    48721 tttcgattgc aaaaccactt ataaacccga ttttttctgc gatatgggaa tctgaccttt
    48781 caagaagggc attaatcggc gtttcaataa aagaggtttc aaacggaaaa accgcctacg
    48841 aaatgaatgc caaaatgcct atgtcacctg cttccataca aaaagttata acacttttgt
    48901 cctccattga taccttaaaa gaaaattacg aatttaaaac agaactatac agagctcaag
    48961 acggaagcat ttatttgaaa ttgggagctg atccgtatct tacgacaaga gatttaagag
    49021 aattaataag catgcttcct caaaaacaag atttaaacaa accgttttac attgacgact
    49081 ctgtaatgga cagccaggaa tggggcgaag gttggcaatg ggatgatgat ttaaaccctc
    49141 ttatgccaaa attcggtgct tataatttgg acaaaaatct tttaaaaata aatttctgcc
    49201 cgggttctca gcaaggggca ccggctgaaa tttccacgga agttttttat cctaccgcat
    49261 tttcaaataa tgttacaacc ggaagcagca ccgaaattaa gcttgacaga aaaaattata
    49321 tttcacccga tgtaataaat gcccaaggta caatctcagg cgattattcc atagaaattc
    49381 ccataaacca ccccagaaga tattttgtgc ttcgccttga agaagttttg cggaagcaaa
    49441 aagtaggata ctatggcgaa tttgaaagaa tgaagcttcc taaagatgcc gttttgctgg
    49501 gaaaaataag ccacccaatt tcacaagcac aatcagatat cttaaaacaa agcaataaca
    49561 tggcagcaga aactgttttc aaactggcag gaggcaaata tataaacaac gcaggctctg
    49621 ccgaagcagg cgttgaaatg ttaaataatt actacaaaaa attaggcata aacaccgaca
    49681 gcataaaaat agttgacgga agcggcgttt ctaaaaataa ccttattaca gctgatttta
    49741 tgacaaatgt tctggtaaaa attgccaaag aaccttctta taacgatttt ataaactccc
    49801 ttgcaaaatc aggcgaagga actttaaccg acaggatgct ttattttaaa gataatctta
    49861 aagcaaaaac aggaacctta tcaaatataa gcgcaattac aggatatttg accgcaaaaa
    49921 gcggaaaaac ttacgctttt tgcattatgg taaacgaccc taaatcaaca aatttcgaca
    49981 aaaaaacttt tgaagaatac gttttaagaa aaatctacga agaactttga acctcagcta
    50041 tgccgtaatt aattgatttt aaagcgtttt ttgcccaaaa tttgtcctgc gctgaaatcc
    50101 tttccccgat ggcaaaaaac gccgaacccg aacccgacat aattgtcttg ggatataatt
    50161 ttttaatttc aagcaattga gcataatcgc caattaatgc cgtttccaaa tcattatgca
    50221 aaaacggaac tatattttct cccgatttta aggcagcaat aagttttgcg ctcatattca
    50281 aattcggctt aaccttaagc tgtgaaaatt ttgtataagc ttcttttgcg cttatgccga
    50341 gattttttgg ttttatcaat gcgaggcaaa attcttgaaa cggcaatctt tccagaattt
    50401 cgcctctgcc tgttgcaagc tgacacccgc cttccaggca aaaattaagg tctgaaccaa
    50461 gttttgcaca tagtttatga agttcttctt ttgaaagcac atccgagaaa atcttattta
    50521 atccccatag agttcctgca gcatctgtgc ttccgcctgc aagccctgca gagacgggaa
    50581 tatttttttc aataaagaca gaaattttat gcgggtgcaa atttgtatgc tctacaaaaa
    50641 gcaaaattgc cttataaaca agattacttt cgtcataagg gatttggtcg cttgtgccgg
    50701 aaagttttat ttcaaatttg cagttttctt caatttttat cgtcagaaaa tcaaacaaat
    50761 caattgtttg cataacactt tgaatattgt gaaatccgtc ttggcgtttg ttcaaaactt
    50821 caagagtaag gtttatttta gccggacatt tgacctttat ttctttttta cccctcaccc
    50881 aaatttctga aactcgtacc tcgtttcgaa atttttccct ctccctcaag gggcgagggg
    50941 gatttaattt tccttctccc cttgcgggag aaggtggctg aaagccggat gaggggtcat
    51001 ttttcatttt aatttcacgc attattgatt aactcctgtg ataatttgcc gaaattttca
    51061 atagaaatag tttcgcctct tgcgttaaga tcgattttga catttttgag tgatttttca
    51121 atattttctt ttgaaaaacc gccgcttaaa aggcagtttt taatattttt tcttctttga
    51181 gaaaaagccg ctttaattgt ttttctgaaa tgcttataat ctaaaagttt aactttgggt
    51241 tctttgcgga cgataagttt taccagtgcc gaatctactt tgggactcgg gtaaaaacac
    51301 ttgcgcccta cgtttttgat tatttcacaa tccgcccaaa actgcgacag aatggttaaa
    51361 agcccatact gttttgaagg gcttttttct gttgccgcaa ttctttgagc gacttctttt
    51421 tgaaccataa gaataatttc ttttattttg tttctgtttt tattatccaa atcgtcaatt
    51481 tcgcccaaaa gatgtgcgat tatagggctt gtgatgtaat aggggatgtt tgctacaatc
    51541 ttgacttttt catcgcaaac caagctcaaa tcagttttta aaatatcctg atgcaggatg
    51601 accaaattgt cgcaatggat tttttttaac tcttttatcg cctcttcgtc aagctctatt
    51661 gcgataactt tttttgcaac tttaacaagc tgttctgtca caaaaccgac tcctgcgccg
    51721 atttcaacca caatatcatc ttttgaaatc tgtgcgcttt ctaatatcgc gcggattgtt
    51781 ttctcgtcaa taagaaaatt ctgtccgagc cgtttttttg ttctgaacga ttttgctctt
    51841 cgtaagtagt ccatgttact aatctataat aatacaaaca gggtaatgtt ttttttaagt
    51901 tttatatgat agtttacatg ttatgattta tttggacaag aagatttatc agcaaaagag
    51961 gatttatgtt gaaatcaaag gatgaaacga ttttatcggt attcaaaaaa ggctgcaaac
    52021 caaaagaaga atactcaata ggtgttgaat acgaaaggct tccaatccat gccctaactt
    52081 acaaatccgt aggctacgaa ggtgaaaacg gaatttatga acttttaaga gccttttcaa
    52141 aacttgataa ttgggaatac ataactgatg attacaacat tatcgggctg aaaaaaggcg
    52201 aagatttgat aactttagag cccggctgcc agtttgaact gagtttaaaa ccggaaaaaa
    52261 atattgccga tttaaaaaca aaaatagata atttcaataa aaaaatcacc ccgcttttga
    52321 gaagatatga tattaacctg cttgaatacg gcgtttcgcc tgtttcgact tacaaaagca
    52381 taaaaattat tcccaaaaaa cgctaccaca taatggcaaa ttacctttgg ggaatcttgt
    52441 ctgatgttat gatgagagaa actgcaggga ttcaagcctg ctttgatttt tcatcggaaa
    52501 aagacgcaat aagaaaattc agggtaacaa acatgctctc gccttttatg acagcaatgt
    52561 ttgcaaactc accaattaga ggcagcgttg acacaggcta caaaaccttt agaggcttaa
    52621 gctggctaaa taccgacagc gaaagatgcg gatttttgag caaaaaattc tttgaaaaaa
    52681 attcagacta cggatttgaa gattatatca aggatgttat gaaaacgccg atgattttta
    52741 taatgcgcga aggaaaccct gttaaaattt tgggcaaaat tgattttgaa cagttcattg
    52801 aaagaggctt tgacggcttt gaagcgactt tagacgattt tcttttgcat gcaaatttgt
    52861 gttttccaga agttagattg agagatttta tcgaaatcag aaaccatgac tgcgcaaatc
    52921 atggcatgca gtatgctatt ttgggaattt ataaagggat tttgtattcg caaaaaagcc
    52981 ttgaagaagc tgaagaactg gtttcaaatt tttcttacaa cgatatagca gaattcagat
    53041 acaatgtccc caaaaacgca cttgaaacaa aaattaaagg gcattttgca aaagatatcg
    53101 caaaagaaat tctgaaaata agccaaaaaa gtttgaaaga acaagggctt ggcgaagaaa
    53161 aattccttga ctcgattgca gaatatacgc ttcaagggct ttgtccggct gatgtaatcc
    53221 tgaaaaactg gtacggcagc tggaataaag acattaataa attgatgaaa tatttgaata
    53281 attaacttaa aatcccttta agcacgatta cgtcttcgta gcgtgcgccg gattccaaaa
    53341 gctcttctgc ggaaaaaccg aaaagcgaga tgagattttt tgcttcctcg tcaattcgtt
    53401 ttttgttgca cgctttgact acctcaccca aagcatcttc ccttgtgagt ttggtaatct
    53461 gattgtttct tccttgatgt aatattcgtt tcttagattt gcccattttc tacccgagta
    53521 aatggtaaat agtgaatagt gaattgaata tttatttttt ctatttacta tttcctattt
    53581 actatttcct tttcaattag tattttcaat ccagcttaaa gctaacaatg ctgcaccaat
    53641 cattccggaa tggttgcccg cttttgcctt gtaaacctta ataggtgccg ttacaatctg
    53701 cgcgtttgtt tcattttcca aataatccgt gtctacaaat tcggcaagag agcccgaaag
    53761 cacaaagcag tcaggatcaa aaaggtttgc aagcccgacc agccctgcca aaatatgatt
    53821 ttgccagatg tcaaaaactt ctttcatttt tgtgtcgcct tttttaaccc cttcgattac
    53881 gtcataagtg gttacattat tgtcgcgcaa aatttcttct gcggttaatt ttagccctgt
    53941 gccagaagcg taagcctcaa agcagtccca tgcgccgcag gtgcacctgc ggcgcatatc
    54001 tgcaaacaat ttaaagtgca tttcaccggc tccgcctgat tttcccttgt acaatctgtc
    54061 ttgtaaaatt attccgccgc ctactcctgt tccgagtgta agcataactg aataaggaca
    54121 attttttgat gcgcccagtt catgctctgc ccaggctgcg gcgtttgcat cgttttctat
    54181 aaaaactttt tttgtcgaaa gcgattgaaa atcaatctga ttgtaacctt ttggtaaatt
    54241 tgcggttgag cttatgactt tgttattttg attgtttaca gcccctgccg ttgctattgc
    54301 aactacatca acctgccctt cgtaagtttt tataacttct tcaagcaaac ttttaagttc
    54361 ctgaatgtct ttaggtgttg aaattttatt gacttcgccc aaaatttcac cctgttcgct
    54421 cacaagtgtg tgataaattt ttgttccgcc aatatctatt gcaagtgctt ttttcattta
    54481 cattttctcc cctaaaaacc gttttgtaat cagctgcggt cttgttatcg cagagcctat
    54541 cacaacacac cacgcgccaa gttcaaaagc cttttgaacc tgtttgggct cccaaattct
    54601 gccttctaac accacaggca cttttaactt ttttgtcagt tttgcaagaa gttcaaaatc
    54661 cggctcatca gattttgtaa gcgaataact cgtatagccc gatagtgttg tcgaaaccat
    54721 atcagcacca aactcggcac atgccagccc ctcttcaaaa gtcgaaacat ccgccattgc
    54781 aaactttccg tatgaatgaa tttttttgat tatatcctcg accgaacact ctcttttcct
    54841 gaaagttccg tcaaatgcaa caatatcggc tccggctttg attaactttt caacgtcttc
    54901 aagtgtgggg gtaatataga caatttcaag ccaattatcg ggaagtttat cgggttttgt
    54961 aagccctatt acagtcagac cgaagctttt ggcgtttttg gtgtcgcgag tgcctgctac
    55021 acgaagcgcg caggcacctc cttgaactac agattccatc attgcaagca tgcactcttc
    55081 cttgtacaaa ggttcatcag gcattgcctg cacagaaacg acaattttgt tttttaattg
    55141 agctaaaact tccatgagtt taattataac caaaaaattt ttttaaggta taatcaaaat
    55201 atgaatatta agaaaacctt agtaaaatac ccgtttttga ccaaagaagt cgaaatttat
    55261 gagcttgaaa acaaccacaa aattgtcctt gcacacaaag agggcgggct tgtcaatgta
    55321 agttcttgga taaaaacggg ctcgattaac gaaaatgacc aaaataacgg tatttcgcac
    55381 tttttggaac atttgatgtt taaaggcaca aacaaatata aagccgggga attcgacaaa
    55441 attttagaag ccaaaggcgg aattgtaaac gccgcgacct ggaaagatta cactttctac
    55501 tatgtaaccc tccccaaagg cgaaaaagac aaagatttat acaaggcaat agagcttcat
    55561 gccgatatga ttttagatcc gattttgccc gaagaagaaa tcggacctgc atttgatgtt
    55621 aatgacacaa aagtaaatga aaaaagagaa cgccatgtcg ttatagaaga aattcgtatg
    55681 cgccaagacc aaccctggac aaaggtttat aacgcatgca acaaagccat gtacacttct
    55741 cacccttata aaagagatgt cataggaact cccgaaatta tttcacaggt tacgcgtcag
    55801 gacataatgg attattataa aaccttttat tcgccacaaa acattacaac tattatagta
    55861 ggtgattttg acaacaagga aattttaaat aaagttataa gcgaatttca ggataaatgg
    55921 gggcaaagag ggaatttaaa gcttacaagc aaacccgatg agcctgtttg tgaaacaaaa
    55981 tatttagagc aaaaatccca aataaattct gcatttatga tgttcggcta cttgggacct
    56041 ttggcgtgcg atttaaaaaa ctcaatcgca cttgaaataa ttgcaataat tttaggcgaa
    56101 ggcacaagtt caaggcttta ccaaaacctt atcgaaaaac aaaaagagcc tgtttttaat
    56161 atgataaacg ccgacaatta ccagttcaaa gacggaaata atttctttat tcaggcaaat
    56221 tttatccctg aaaagaaaga tttggcaata aatttggtaa aatcagagct tgaaaaaatt
    56281 gtaaacgaag aagtttctca aagagaattt ttgaaagcaa agaaaaaaat caagtcccta
    56341 ttcgcgcaca acgccgaaac agtttctgaa atcggtgaaa ctataggtta ttacatgacc
    56401 gtgtgtgaaa atttggctct tgttgaagaa tatcttaaca ttttggaaaa aattacacca
    56461 aaagatttaa aagaagcagc ccgaaactac ttgccagtaa gcaatgcagt aatttcgata
    56521 cttatgcccg aataaaattg tatatttttt gtaaatatat ttgtattttt tgcttgaaag
    56581 tagtagaatt agaaaataat ttggttattt ttaacttgag agggtaatat atgaaattac
    56641 acatgcagat tctgatagct ctcgggcttg gcttgaagcg taattggcac atattttttg
    56701 gcttaatagc ggggattttt gtagggattt tcatgcacag ccatgacagt gtttttctga
    56761 caaagatact tacatttatc ggtcagatat ttataagact tattcaaatg gtagttattc
    56821 cgttggttat ttctgccata gtaataggaa ttacaagcat cggcgacaac aagcagcttg
    56881 gtaaattcgg aacaaaaatg attatctatt acggtataat aactgttctt gccgttacaa
    56941 taggtgcggc tcttgctctt acaatgaaac ccggagtcgg tgcagcacaa tacattaatg
    57001 ttgatgccgc cgcttcaatc caacagcagg ttgcaacaac aatcgaacag caaaaaggca
    57061 atgttttaaa tatttttata aactttatac ccaaaaatcc tcttgaatca gtcgctaaag
    57121 gtgacatgat ttctatcata gtgtttgccc ttatttttgc tgtagcactt gcaaaagtag
    57181 gggaaattaa ccgccctgtg gtgtcgtttt ttgaatcgat ttttgccgcg acaatgaagg
    57241 ttacagactg gataatgtac tttgccgcac ccggtgtttt tgcgcttacg gcaactgcag
    57301 tatcgagttt tggtatagac atttttcaaa gtatttcaaa atatttggcg gttttatgca
    57361 taggatttgc aatacaattt tttgttgttt atccgatttt cttgaaagta ttttcaaaag
    57421 tccctgttct tatgctctat gctgcaatag cagaagccat gatggtggca ttcggtacgg
    57481 caagttcttc tgcgacactg cctttaacta tcgcatgctg cgaaaaaaga ggaatttcac
    57541 ataaagtttc cagtttcgtg cttcctttgg gtgctacttt gaacatggac ggaactgcac
    57601 ttctgcaaac agttgcggta attttccttg cgcaagctta tggaatccac ctcgggccgt
    57661 ttttgattat tcaaatagct ttccttgcaa tgattgcttc aagtacttgt gcgggaattc
    57721 cgggcgcagg gcttataacc attgctcttg tgcttaacgg tctgggattg agccctgaac
    57781 agcttgttgc aggctttgca ttcctgttta ctatcgaaag gctcacggat atgatgcgaa
    57841 ccacccttaa cgtaacctca gatgcggttg tggccgctgt aatcgccgat aacgaaaacg
    57901 agattaacta cgatttattg aacaatcccg agtcgtataa ggaaataatc taagtgagaa
    57961 gcaaaaagaa gttagaatca attaaatctt taggtaaaac caaaaccgat tacccgacag
    58021 aatacgatcc ttccgtttta gagtgctttg aaaacgttca tccggaaaat gattatgtgg
    58081 taacgcttta tgcttatgaa ttcagtagct tatgcccgaa aacaggtcag cccgattttg
    58141 caaaacttta tattaaatat atccccaata ttaaaatggt tgaatctaaa agtttaaaat
    58201 tatacttatt cagtttcaga aaccatggag attttcatga agattgcata aatatgatta
    58261 tgaaagattt ggtaaaattg atgaaaccaa agtattttga aatcaccgga atcttcaccc
    58321 ccagaggcgg aatttcaatc acaccttttg caaattattc aaacggaaaa gaaaaataca
    58381 ataaactcag ggattcaaga cttttagaag tattaaaaga aaaaactccg ccaagtattt
    58441 cttgagcata aaattagggt ggattcccca acccaccaat caaggatttg ttacatgaca
    58501 accaaaaaca aagaaacagg tgcaaaggg
//
LOCUS       DAOQAU010000008        42383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_781_length_42383_cov_7.583940, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000008 DAOQAU010000000
VERSION     DAOQAU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 42383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42383
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_781_length_42383_cov_7.583940"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..698)
                     /locus_tag="PKI94_02520"
     CDS             complement(189..698)
                     /locus_tag="PKI94_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNW25645.1"
                     /translation="MAIKTTKGKDALFTEINITPLTDIFLVLLIIMMVVAPTFQSMDN
                     SINIPEINSGVSVEQKDATVAVTKEGVMYINGKQISADNLTNELLAIKDSLEKKEVVV
                     KADEKTKSSEIMKIMNAAKEAEFTKLVLAGEPLSKKDQKTLSDGNNKRIKPIEKQPEQ
                     VNDTQNWED"
     gene            complement(806..1453)
                     /locus_tag="PKI94_02525"
     CDS             complement(806..1453)
                     /locus_tag="PKI94_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNW25646.1"
                     /translation="MELLLHSIKMDWPVLLPILVCSILVVAVALNRYSFYNKNKRDVV
                     QFIPKLQKELARNNLDSAQTLSVQLGGVIGEVSEEAVRIFSEQRKGFSRSFDIAASLA
                     TRKLEHHLTILGTIGGVAPFLGLFGTVVRILYTFQDLATQGNQSAAVAMGIGSALIAT
                     AFGLGVAIIAVIFYNSFQSIVKRFEDDFQLIKLLFLSFVDSEEEVKVQSASNIAL"
     gene            complement(1493..2641)
                     /locus_tag="PKI94_02530"
     CDS             complement(1493..2641)
                     /locus_tag="PKI94_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNW25647.1"
                     /translation="MAILGNINSLIDDDDIKEKESYTIPSVLKHSQEGISFKKSLVLS
                     TVLHPAVVGLVWLIILILTLMGITLSIFEKPKPKMNDIEFVLVDKEQMPLNKKTPYRA
                     DRNSRTGGKRDPKKKVSMPSPSPTKKAVQQQSRSSQSAKKPSVKKPQAQKVPQKKNVF
                     EQLTQKQPQARPQPPSARPSIKPPSVPRPSVKPNPEFAVPIPKASSSSTGKTYSTGPI
                     GGSGSAKTGSSGSGRSYAPVPSLSPSGGSGSSAGSSGSKMSKGSSGYGNYGNPGGGGG
                     APGIDAIREPDFGPYMRELQRRIKMNWDPPKGNESKRVILLFKIARDGRLLSIKVFKS
                     SGVPSADKAALSAVELTAPFKPLPPDYKGSSIDIQFTFDYNVFGATNY"
     gene            complement(2819..3568)
                     /locus_tag="PKI94_02535"
     CDS             complement(2819..3568)
                     /locus_tag="PKI94_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25648.1"
                     /translation="MNKKTFSLILLASMAGANLSFAASNYQYQPNFPQPQYQQNYYPS
                     EVRQTGNQPLKGRVVTVPPGITMSAVVSTPISSEFLTLGQPVSVTLGSDFYYNSTLIA
                     PAGSTVMGTVTQVTKAKHGTINGSLRLRFTEVTTPYGARIPISAMIKTDDGTGLLKGG
                     TKMDTAKEYAKDMAVGSAAGALTGLVASAIAGGAVGKGTAIMTGVGAGAGLAKSIYDK
                     GQDVIIPSNATVQLMIDQPITVNPSGYNYDY"
     gene            complement(3638..4207)
                     /locus_tag="PKI94_02540"
     CDS             complement(3638..4207)
                     /locus_tag="PKI94_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25649.1"
                     /translation="MKRLFLLLFLMISFALPCLCEIQLRGYEGAEIPKGTFIPVISTQ
                     EISTQYCDEGTKVKFIAVSDLYLYDTNVIPQNTEFFGYVEKINEPVVGTNGSMVIKVI
                     KFKTPQGYETPIRAYIYTPKGNLIGGELTNPATYDKKPHFQQGFSHGTLQYVPGATRQ
                     MGEHTVVASGADLIIVLAAPVFITQGVTN"
     gene            complement(4376..5329)
                     /locus_tag="PKI94_02545"
     CDS             complement(4376..5329)
                     /locus_tag="PKI94_02545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW25650.1"
                     /translation="MRKPTLAIIVPCFNEELLVESTVSSLFEVINALIEKGKILEDSY
                     VYLIDDGSKDRTWEIIEELHRKNQLVKGVRFVKNYGNQKALMAGLLGARELGCDCVVS
                     IDADLQQDQWAIEKFVDKYSQGYDIVSGIRNRRDTDSFYKKYTALFFYKLMNLLGAKI
                     PVNHSDYRLVSKRALDILARYTEKQLFLRGFFHEIGLKTAYVNFDVKPRMLGKSKFNF
                     ISLLGLALNGITSFSIIPLRIIAVLGFFMALFSFGLGLQVLYEKIVLHSTPNGWATQV
                     VFLTFFGGIQVFCLGIIGEYLGQVYREVKARPRYIKDIELY"
     gene            5401..6252
                     /locus_tag="PKI94_02550"
     CDS             5401..6252
                     /locus_tag="PKI94_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNW25651.1"
                     /translation="MSNKKFILNADDFGMSNAFNRAVLDGYNHGFLTSASICANGEAF
                     DAAVNEIIPECPNLGIGVHLNIIEGYSLSNFTKMNMLCDLNSKFVKSYGQMIYLSGNK
                     DFMAQVETEFRLQIERVLNYTKIDHLDSHVHVHAIPKIFELTCRLAKEYEINQVRTQF
                     EKFYTVPDIKFHLNLKYPPNLLKIMLLNYFTLINRQKIKEYGLQTNDYLIGVGYTGMM
                     NDKTLYCGLETINQDCTVEALIHPCNHINSKKAKSQHQIEFNITQNMDLKDKIQRLDF
                     EITNYKS"
     gene            6273..8060
                     /locus_tag="PKI94_02555"
     CDS             6273..8060
                     /locus_tag="PKI94_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNW25652.1"
                     /translation="MKGFTVTILIFSFLVFLAYQFQKTAEFDVLEITSPIEFTVDLNN
                     NGIKDDDEEIFLEDIKSFSTKPSQSQTELAKKIGISQEDALGLGFLAQNFAKENLSKK
                     VKFTGKEDKKILIDNKVYEKMLLEQGFATSKSYANKDKIQQNILKAKKLNLRIFNNKS
                     LKYHKLNCKYGLLAHNSQILPLNQIPKDAKACQFCSGKQKEKNKSKKIITIYEEKIPY
                     APKAAGIFNGRGIEIYLPDLTSVLKPSNKCNTDFCRALLREINCAQSSIYFAIYGYTK
                     TPAIQSALENAQKRGVKVRFVYDIDGKNQNLYPDTLYLTSVFKNNNADFAVEKKYQNI
                     IMHNKFFIFDNKKVLTGSANISDTDLSGFNSNAIIILDSQQAANVYSQEFEQMYGGKF
                     HNTKQKICNKEGLQVGDTEFSVYFSPTDRTMNSKIIPLIDSAQKYIYMPVFLITHQGL
                     CDSLIRAKNRGVDVKVIIDATNAHGTYSGHKLLRQAGIKVKTENYAGKIHSKSIIIDD
                     LYTIIGSMNFSRSGDNKNDENVLVIKNPDIAVFYKKFFQYLWLRIDDKWLYKNARAES
                     PDSIGSCTDGVDNDFDKKIDMQDESCRAY"
     gene            8180..9349
                     /locus_tag="PKI94_02560"
     CDS             8180..9349
                     /locus_tag="PKI94_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNW25653.1"
                     /translation="MNNYEAGYYIKEKDFRAFVPYPINKPFEIYDLKIISLLEKAGRL
                     IGELNAYSKNLPNIQSFLWFLSLTEANSSNKIEGTKITLNEIISPEEDILPELRDEKI
                     EILNYLVSLEKSYERITGTAGILTTPKKSNVELLPFCTRLIKEAHKILLTNSRGKNKN
                     PGKFRTSQNWIGGTMPSTASFVPPPCDKIPELLSDFENFWHNDDLNIPILIKIAISHY
                     QFETIHPFLDGNGRIGRLIILMQLVDSGILELPTLYLSKYFEKNRKQYYDALNLVREK
                     NDINQWVKYFLEGVCDISKYTVIKLKEVNDLNNLCEEKIKKLTKSHVNASRLLKLLYK
                     NLSVKVNDVARELNLNYQVANALVKAFVKMGILHKGKQKRNTSYIFRDYFNLFVD"
     gene            complement(9346..9525)
                     /locus_tag="PKI94_02565"
     CDS             complement(9346..9525)
                     /locus_tag="PKI94_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25654.1"
                     /translation="MKKQFQTCFHCEYKPLCKVGKSRLEGLDAASPAISDIGCFDFEI
                     YKRQTQDKQLTLGFN"
     gene            complement(9651..10952)
                     /locus_tag="PKI94_02570"
     CDS             complement(9651..10952)
                     /locus_tag="PKI94_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNW25655.1"
                     /translation="MSVIEKFSDISDVMSGLIDFVQTDDEVKADFTEYLSTVGVKTLS
                     ENQLQSVCLPYIFERNLGSDSKSIIELFLEKTKKITKGARLILKALTGSIYSVFEVKK
                     ILKNGFEFYNLVNEKDYSVISLVKMTHFRGVGVGQYVVARIFEFEGEYYLLEISNVLA
                     ANQKEDAMRFAIAKIIQSPEIVYKDNPKKQKEIEVQVKKLHKAFLECFGKEEIVTTNK
                     LADDIIGLFNDFAEGAKKPLAEEIKSKIKTVDEHKFFHVQEFNNSYENFLENSLGGFS
                     AHKETYDVGVIFDEELGLSAIPFYQTFCKIFEEKDYKTIENYEKCVEHFLLTDSVSAN
                     LLRRVEAKYKNFMKVINKIYSTEMTFDELLEKYKKQHLTEKIFSSTTVLYESKIFAKT
                     LGYIEEEEQKPKIEAQNVGRNEPCPCGSGKKYKKCCLYAHA"
     gene            complement(10949..>11335)
                     /locus_tag="PKI94_02575"
     CDS             complement(10949..>11335)
                     /locus_tag="PKI94_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNW25656.1"
                     /translation="IHAGWRGTAAKIVQKAVKKMQEEFKSESEDIIALIGPAIGKCCF
                     EVDKDTMEKILSSVKDEEKVLVCDKMNVDLKLVNKFQLLAMGVEKIDLCEDCTCCKNE
                     LFFSYRKEKGNCARHSLAVMLKRGEK"
     assembly_gap    11336..11435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11436..11659)
                     /locus_tag="PKI94_02580"
     CDS             complement(<11436..11659)
                     /locus_tag="PKI94_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25657.1"
                     /translation="MFKQEEFMGRRVLKSSFLEDEPVIAFFTTRELPLKCGERQDLKG
                     QIEQNKKLVCEGLKIPLENLIIPNQTHSDNV"
     gene            11863..13224
                     /gene="ffh"
                     /locus_tag="PKI94_02585"
     CDS             11863..13224
                     /gene="ffh"
                     /locus_tag="PKI94_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNW25658.1"
                     /translation="MFDSLSEKLQEIIRKTSQGTLTEDNMGEALREIRRALLEADVNL
                     RVVKAFITNIKEKAEGEEILKGINPSQQLVKIVHDELVDVLGHQVSPLNLSGHPSIVM
                     MLGLQGSGKTTSSAKLAVKLKKEGKNPLLVACDVYRPAAIKQIQTLGQQIGVEVFSDE
                     NSKDVQKIVQNSINYAKENGFNVLILDTAGRLQIDTDMMAELLLIDRAFVPHEKLLVI
                     DAMTGQEAVNVAQNFDAQLNLTGLVLTKLDGDSRGGSALSVVYCTKKPIKLTGTGEKL
                     NALEDFYPERMTTRILGMGDIVSLVERAQEVFDEKEALELEKKMRKAEFSFDDFLKMQ
                     KQMKMFGSLDQLLGMLPIPNLKKEDKEMISHQGEKQFKKIEVFIQSMTPQERANPNLI
                     NSDRKKRIAAGSGISLHDINIFIKQFEQMRAMMKGFSDVKDKMKGGKMNPAQMMSMMK
                     RFK"
     gene            complement(13217..14581)
                     /locus_tag="PKI94_02590"
     CDS             complement(13217..14581)
                     /locus_tag="PKI94_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNW25659.1"
                     /translation="MFEGLIINSINNVLNFTQEKALYLSQNSLFIKHIDGLIFASILA
                     VLGLSIFMPSDALGYVALITLFLTVIKLFVKKGEKIEGNLFELLLLVYFIFVVISLSG
                     STLFHLSLKGFLKTFVYLGFYFSAVQYFKNNLSKIPFAIGLIALCAGIEGFVGIFQNF
                     GQVGEIATWQDVTNINPEDVMTRVYGTLQPFNPNLFGGYLVAVLPCLFGFSAMRFYEK
                     HYKLGIFFLVLALVSSYALILSGSRGAYLGFLAIIVGIFAFLAKFIWSDLEKNREFFK
                     KIYVSLAAFAAALVAFAVVFISSVRTRVLSIFAMRADSSTSFRLNVYHSALEMIKDNW
                     LFGIGVGNQNFREIYGLYMKTGFDALSCYSVFLEVAVESGIFALIAFLGFLGVFAKQT
                     FKFITQNSDFKLCLIVSISLISVFAVMVHGLVDTVFFRPQIQFVFWTLTACASSVLRK
                     AETI"
     gene            complement(14679..15281)
                     /locus_tag="PKI94_02595"
     CDS             complement(14679..15281)
                     /locus_tag="PKI94_02595"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HNW25660.1"
                     /translation="MENKKLKLIDIIVILGVFIALVVGFLTYKNVRQTASNKIEGEAK
                     IAFQVFLRGITLTTGQSPIRFGDKTFISIRNVPYTELQIIDVKADSKKTIMPSTRTKE
                     PFVMVEDLSQPFMYDVVVTVIDDAKITDDGAVVGGNKVKIGLPIVLEGKDYKFNGLVS
                     NVQILSKEQVEQIKKVLKDYQAKMAQAKQNPQLAPVQEKK"
     gene            complement(15295..16305)
                     /locus_tag="PKI94_02600"
     CDS             complement(15295..16305)
                     /locus_tag="PKI94_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNW25661.1"
                     /translation="MDVDKNKLIECIKNLRLPKVLVVGDLALDEMIYGDTERISREAP
                     VLILQHTHTKLILGGASNAANNASVINGGKVGVIGVLGEDYQAEQLICAFHSAKIDTK
                     YLVKDKNRKTTTKTRISGSCSQSITQQIVRVDRQTNTFLSEDTEKKVIHNLEHAIPEY
                     DAIIISDYHIGSLTQKVIEKAIEIANKHNKIIVADAQKNLENYKDVTSMTPNLPDTQK
                     FVGFEVKDDVLLEKAGRKLLEVSGAKSVLITCGSEGMVVFNENGEMTKIPVFNKSEVF
                     DVTGAGDTVTAIFTMALASGAQPKYAAIIGNIAASIVVRHFGCATTTVEEILENLEKI
                     AL"
     gene            16394..17056
                     /locus_tag="PKI94_02605"
     CDS             16394..17056
                     /locus_tag="PKI94_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25662.1"
                     /translation="MSNPEEEKELGVKTESVLTQEGNDIEFEDTIDIDKIQQSLQKHL
                     EEDKKNEEVEQDIIQEQEFADEEIQQTTEKAEPEIPAEIVSLQSEQELPKLLQNATPE
                     IDPNAKKYVIYIDPENIPFMENLSVSERKDIINTILKEQEETVRKQKAINQRKVFMRH
                     IIFASIVFIFGFPILFFVVNKSMEAVMSNYQQAEKNFSTLYKQKGKVQPFSPKAAEKF
                     KY"
     gene            17144..18532
                     /locus_tag="PKI94_02610"
     CDS             17144..18532
                     /locus_tag="PKI94_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNW25663.1"
                     /translation="MLRLDYRNASSGIIGNEHGLEIEKEFENHKERISSIISDLNKRK
                     DKPGQWLQWMNLGYSEETIWYVKEYASLVKDRFENILVLGIGGSALGGIALTEALLKP
                     YWNLLDSEQRNNFPRIFFLDNIDPDQIAGLLEILDLQKTLVNVITKSGSTAETMSQYM
                     IVKDIMERELGDNYRRNIVATTDKNMGILRQLADQEGYKTFIVPDDVGGRFSVFSAVG
                     LLPFALVGLDIDEITNGVKDMDLALKNTDVFENIAAQNALIHYLLDTQKGKNISVMMP
                     YSSRLKYIADWYVQLWAESLGKETDRNGEKINCGPTPVKALGATDQHSQIQLYNEGPN
                     DKVINFVRVEEFDNKVEIPKIFEYTGIGYLGGKTINDLINAEADATKVALSDFQRPNI
                     TISIPKINGYYIAQLLYMFEVQTAIAGELYNIDAFNQPGVEQAKNYTYALMGRAGYED
                     SAQTLKEKMAII"
     gene            18738..20096
                     /locus_tag="PKI94_02615"
     CDS             18738..20096
                     /locus_tag="PKI94_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNW25664.1"
                     /translation="MSNELKRTLTLTDAISIVAGSMIGCGIFIVSADIARQVGSTWLL
                     LLVWALAGFVSFCGAIAYGELAANIPDEGGQYMYLKKIFNDKLAFLFGWTLFLVIQAG
                     SIAAICVAFAKFTGILTPLISSHVALFHLGPIAITSQKLFAMATVLLLTFVNSRGIEY
                     GVITQNLFTVTKILSLIGIILCGIFFGMNWSVIHANFATSTFSFHFSDIAKISTATVG
                     ALFASITWNNVTFIAGEIKEPEKNVKKSLAYGVGLVVILYFLINVIYVSSIPLNMIQN
                     APEDIVAAATMGAIFGSIGKHLIAIVIMISAFGCANGMILAGARVYYQMAKDRLFFRS
                     LAVINRQTRVPVNSLWLQCAWICTLIVWGNYTKLLDFVIFASLIFYIITMIGIFVYRK
                     QHPDEDKTLVVNNFFPLAFIIISTYIVICLAMYKPLYTLPGFLITLAGLPVYFLWKRA
                     KK"
     gene            complement(20113..20265)
                     /locus_tag="PKI94_02620"
     CDS             complement(20113..20265)
                     /locus_tag="PKI94_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25665.1"
                     /translation="MEEEKETLTFDDIDILMSVGSDLCEAIRDLIYNIREIKQDVDNI
                     KQHLKL"
     gene            20364..20573
                     /locus_tag="PKI94_02625"
     CDS             20364..20573
                     /locus_tag="PKI94_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW25666.1"
                     /translation="MNKKVKNKLANNIKKLRKEKRISQESLSLELDFDVSYIGKVENE
                     KMNISIDRIIKIADFFEVNFEDLFK"
     gene            21759..22169
                     /locus_tag="PKI94_02630"
     CDS             21759..22169
                     /locus_tag="PKI94_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW25667.1"
                     /translation="MEKTSLAQFVQEKRENLGLSVKGLAKKCHLNLDLIEKIEAGEEL
                     FLSTTVRQNLAKVLKCEPAEIKELEKDFENKFVSQEIIDELKENILAGKTDLTCPNCG
                     SNLITRIAKLYDLEDNLMLHPKARCSKCVFQIKN"
     gene            complement(22239..22592)
                     /gene="rplT"
                     /locus_tag="PKI94_02635"
     CDS             complement(22239..22592)
                     /gene="rplT"
                     /locus_tag="PKI94_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNW25668.1"
                     /translation="MMRVKRGNVSRKRHKKILKLAKGFIGARSRIFKVANCAVMKALK
                     YQYRDRRVLKRNMRRLWIVRINAAVREQGMSYSRFVNACKKANIIVNRKMLADMAVND
                     KEAFSVLVEAAKASA"
     gene            complement(22597..22806)
                     /gene="rpmI"
                     /locus_tag="PKI94_02640"
     CDS             complement(22597..22806)
                     /gene="rpmI"
                     /locus_tag="PKI94_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNW25669.1"
                     /translation="MPKMKTHKSGAKRYKVTANGKILRRHAGIGHLLANKSSTRKRRI
                     SGMEEISETHVDLVSKQLPYKKYAR"
     gene            complement(22932..23516)
                     /gene="hisB"
                     /locus_tag="PKI94_02645"
     CDS             complement(22932..23516)
                     /gene="hisB"
                     /locus_tag="PKI94_02645"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNW25670.1"
                     /translation="MRKSTKTRKTNETEVKIELNIDGKGKMEISTPIKFFTHMLEQFA
                     AHGGFDLKIDVKSHDKDNHHVIEDVALTLGEAFKEALADKKGIVRYGSFVLPMDEALC
                     LAVIDLSGRAFSNVDVEIKDEKTSDFETVLLPHFFNSFAQASLSTIHIKLLEGTDTHH
                     IIESVFKSFARALRQACSFDEKNKDSIPSTKGIL"
     gene            complement(23500..25221)
                     /gene="hisC"
                     /locus_tag="PKI94_02650"
     CDS             complement(23500..25221)
                     /gene="hisC"
                     /locus_tag="PKI94_02650"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNW25671.1"
                     /translation="MKAKKTIQKIEPYQVDLFDEEGFLKLDSNENDFGPSPKVVDAIK
                     NISPQDIKYYPYYGKLIQKLADFHGVGIENIILTAGADEAISSIFSTFLESGQTVLTV
                     EPSFVMPRIYAKVNGLNFKEISYKKKWEFPQEEFLKNIDNADLIHLTTPNSPTGEIID
                     RKVIEKIIKKANGKAILIDETYANFCEISNLDLARKNENIFIVRSFSKDFALAGLRLG
                     YVISSEENISNLRKYLSPYNVSNLSVKAGLAALDDIQYFKKVKKEIEVSKEMLIKGLK
                     KLDNTIYPYPSETNFLCVDFGQKAEFIYKKLLDAKIKVKYFKNIPLLENTMRITVPTL
                     ENTKKILSVLEKKTAIVFDMDGVLIDASKSYRVAVQKTFEHFAKKEFSLEKISQTKKL
                     GGLNNDWDLTEYLLKSEGLKIDYDDIVEVFQGHYKTLAQIEEPLVSAEFFEELSKDYN
                     LVVFTGRLVEEAYYSLDKNNFTKYFYPIITMQDVGLNRQKPDPLGLEIVKQKIIADEI
                     YYLGDTVDDMICAQKAGVKAIGVLPPQDKSEDLKNLLKSKKAVVVLEQTVDLKNYLKE
                     GRYAQKH"
     gene            complement(25243..25929)
                     /locus_tag="PKI94_02655"
     CDS             complement(25243..25929)
                     /locus_tag="PKI94_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25672.1"
                     /translation="MSPNIMPVFNKLLDSKLLNKMYKAAKDNPAKFAAQMALTSALTK
                     DAVGCYYYVTQSLHNKKIPEENRNFVASIDLMNGILNVLLQFTVGSWLDKAAPKFFDN
                     LMEKKLGTNKTRQIAKTLEEIINNKEITAVKIENFLKNKMLGKGGKTAKWLKVGFSAV
                     SMLVATQIITKRMIVPFLSTPLAGWAEKNIFKKKDAPKVNIEPLNNKLSYQDNSSSGR
                     TNLLSLYSKK"
     gene            complement(25926..27302)
                     /locus_tag="PKI94_02660"
     CDS             complement(25926..27302)
                     /locus_tag="PKI94_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25673.1"
                     /translation="MSKVNLVQQVQSTNFKEKPLKKPQVSFKGKEDVLTAAQELIVEQ
                     ALKTKYLGFFGRTLDYFSKTAGEIQNLLLIGFGTAFVAPIFIAYNSLSKQDPKKKKYS
                     AMRQPISAVIATALGFGINKPIADGLSKACSEGALEKFDMSAKPQSDFLKARYNSIVK
                     NFGKLKDLDKKYFEKAGTQDFKDVKAFREKFPTFQHFEQAVHKTTLEEVAQKLLSESS
                     QNSIKNQTLRDFLIKNMDFEKDCIDEKMLNPYMVKSKLEKTTAIDFLRKIGFDDATID
                     EKSLRKALNRIISTKGFDSNQKDIIEQIAENLITEEAKGKNNISMKALFNILGIEKDF
                     HQSPVLDSTLDKFMLTMHEMLSDSTSSSSRKTFTETLKEFNVKKILTENDISRLKNFA
                     KQLAKNSVDDATTAFKAYTKAQGIVLSLITLPFACGFLNWSYPRIMDKFFPQLCPAKK
                     PQEGGKTL"
     gene            27431..28924
                     /locus_tag="PKI94_02665"
     CDS             27431..28924
                     /locus_tag="PKI94_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNW25674.1"
                     /translation="MRRYFLIILLLFLSCAKSFAFEIVYPKKTPCTIDAPSTFFIGST
                     NPQDILKINDAEVPLHKSGSFAFVVPLKEGINEFKITSYPSPLPSSYGTPHPNPLPQG
                     ERENNYNKIISARGEGEINPVSIDFIIERPKPQTTKKIEPILLEYPPMNNFFIKDDNT
                     PLRLTPFDSGINRMAHLQKETPLVINGEKGGFWRVFLNSKTIGWILKSDVEQKTDSTI
                     SALPVSIKKLETKETKDFCIWEFELEGKTPFVLKEEDNRLNLKLFNVKSEDKNLCIED
                     SSLNFDVPVKKLFGYDAYFEGNKFILKVRKAPEKNCDMPLKGLTITVDAGHGGAEYGA
                     IGPKGDKEKDINLEIAKNLKAELETLGAKVIMTRKDDIAVSLKDRVKTARENDSVILI
                     SIHANALPDGADPIKCRGTSVYYYHNQAKPLADSILNSIITKSGTQNDRMRQGSLALV
                     RPTSSVSVLVEVAYIINPDDCELLLDKEFQKNCAKAITEGVVKYLSD"
     gene            complement(28913..29479)
                     /locus_tag="PKI94_02670"
     CDS             complement(28913..29479)
                     /locus_tag="PKI94_02670"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNW25675.1"
                     /translation="MKEKLLAELKRKCSECKACELHKTRNNVVFSDGNPESAKIVLIG
                     EAPGENEDLQGIPFVGRAGKLLNEFLEKAGISREKDLYIINMVKCRPPNNRVPTKKEK
                     STCRHFLTEQILTINPKLILLCGSTAMEEFLEGKKITEVHGKVFEISVSNKTYKAMPI
                     LHPSPLCRYPDKTNVMVKDLSSAKKLIG"
     gene            complement(29436..30950)
                     /locus_tag="PKI94_02675"
     CDS             complement(29436..30950)
                     /locus_tag="PKI94_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HNW25676.1"
                     /translation="MILNVNLDAAIDNFLSPVADKISALIFCHITIGGVQVQLLVALL
                     MFAALYFTIRTRFIGFWGFKHALNLISNKYKHPAVSKKKKIGEVSSFQALSATISASA
                     GMGNIAGTAAAISLVGPGVLFWMILAGLFSMALKFSEVLLGIKLRKVNPDGTVAGGPM
                     YYILKGLKGKYLSKIAPHLAKIYAICCVFAMIGGWNLFQINAMTTQITEVTGGNASIF
                     HNNGWALGLIVALIIWFTIIGGIKSIGKFTEKVTPVMCSLYLLTALVVCLMNFERIPY
                     SFVLILKEAFKPQAVAGGMFVSMLWGFRRAMFSNEAGLGTASIAFSAVKTSKPVAQAF
                     IAMLQPFVDTVIVGSATALVIIVSGEYLNSSGLAGIELTSRAFGSAFSFFPLLLTVMV
                     SCFVTSTLLCGSYYGLKAWHFLFGESGKNTKVFQVIYCVFIILGSAMNFKSIINVSDA
                     FTLFLAVPNLFAVFVLSNVVIKELKKYCRKYSIGIFKEKSNEREIACGIEAQVQ"
     gene            31198..32256
                     /locus_tag="PKI94_02680"
     CDS             31198..32256
                     /locus_tag="PKI94_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25677.1"
                     /translation="MKKTGLTFTEILLSFGIIGILAAIILPVLQSTRPEPNVVMFKKA
                     HKIFVKNVFDLINNETYYPSNKTGLTAGNLLKPRGFNYTDLEGLPDKTDKFCYLFSQK
                     INNLNEVRCKSPGNGYFTTTDGIYWEMKLPFDFPMSVNGYEQVMVDVNGPQNPPNCEE
                     SYCKKGIVPDKYPLYVRYDGKMKTSLLGGVITINPTIQTKKVLTITPQPDTPEVEPTP
                     PDTCTPKGNIKVGNLCVSPTNIIYVPVSYPPYNDKDNYWKGADNACRAYGMRLPTKAE
                     LQDMYKQRKEIGNMNPGWYWSGEEISSDENPTGPPSTWDYAGAYDFTNKGGNVVNGKH
                     DQGGRARCVLQWNGAGYY"
     gene            32698..32913
                     /locus_tag="PKI94_02685"
     CDS             32698..32913
                     /locus_tag="PKI94_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25678.1"
                     /translation="MYSKIEKINKLDIASSLTNQAIRMFFHKDNPISTHVIVSAANEI
                     LINLIKKNGLKTNLKSKYGRFSNESKV"
     gene            complement(33054..33449)
                     /gene="ssrA"
                     /locus_tag="PKI94_02690"
     tmRNA           complement(33054..33449)
                     /gene="ssrA"
                     /locus_tag="PKI94_02690"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            33737..34933
                     /locus_tag="PKI94_02695"
     CDS             33737..34933
                     /locus_tag="PKI94_02695"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNW25679.1"
                     /translation="MKTNKKINIKPADKILNLEKYIFAELDEWKQEARAKGADLIDLG
                     IGNPDGPTPKPIVKAAIKSIKNPACHGYPCFRGKQEFQNAIKEWIKFRYAIKLDSDFG
                     IQTLSGAKEGLAHTALAFTNPGDINIVPDPYYPVLSRGTWISSGDVYHVPLKAENNFL
                     PDLDSIPKDIAKRAKMFFINYPNNPTSAVATKEFLKKLVDFCTKNNILLVYDLAYGEI
                     CYDGYRPPSIFSIEGAKDIAIEFHTFSKTFNMAGWRVGFVIGKKEFIDAIHAMKTNTD
                     YGTSTIVQDAAIEALKMPYENVEKIVKKYQKRRDFMVSAMKKLGWEVNKTPATMYLWL
                     KVPKGIGSKEFCRKTMDKTGVVFTPGVAFGKHSNGFFRVSLVQSLSKLKEAVKRLEKA
                     NVRYEE"
     gene            34935..35669
                     /locus_tag="PKI94_02700"
     CDS             34935..35669
                     /locus_tag="PKI94_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25680.1"
                     /translation="MKIYSKIFIILSVCALGTTLLPANAKFNAGISKNHFYDTQKTIM
                     KEISPNIVPNTDNYWEKNTFFTNKYLPLFMLSNKDLISYLPPKKHKIINRMIIPSVYN
                     VSFKTAPVTNVSLGGGKKNEVVSNQEFDVKWMMLYEQAKNKEADSDKKIETAVLLKDT
                     KNISNYNLAIDLLDDVTRQEPYNAYAYNLKGDIFFVKNDKENAMKNYVEALKLNPLSK
                     QSCLGIAKILEPTNKELAQKYFEKAK"
     gene            complement(35673..36233)
                     /locus_tag="PKI94_02705"
     CDS             complement(35673..36233)
                     /locus_tag="PKI94_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25681.1"
                     /translation="MKVNIISYQTFMRKKPAENKRQNNEPLHFPIMLNCSDLSKEKAK
                     EQYRILQNAIIENKQEKALEIIKHFEKLPKNIQSCIPIVQNDNNENIFILAVKNNQPK
                     VALKVLDFVKTLPEKAKIDFAFIRGRNNENQSYIAGEYSQRIVRDAFGNFVKTLPKDI
                     QTITYQYYGPRQYYNDSTAYGDDLDW"
     gene            complement(36324..36854)
                     /locus_tag="PKI94_02710"
     CDS             complement(36324..36854)
                     /locus_tag="PKI94_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNW25682.1"
                     /translation="MSDYSKEDLYNIIKASPQRFNEWKKGQDEVDLSEMNFSYCILSD
                     VDFSDVDLNSSSFADAHLTSINFSRADLTSVDFTRATILECDFSEAVLTGADYSYAAI
                     NYCNFPEADMAGGVFQESDLSNSDFAGCFNLNACRFDEETIWPEPEMLPEDFDATYSD
                     DLSLLKDDEEDLLQEY"
     gene            complement(36876..37505)
                     /locus_tag="PKI94_02715"
     CDS             complement(36876..37505)
                     /locus_tag="PKI94_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW25683.1"
                     /translation="MSQLARILIVDDNPKYLQDALPMYGYEVKVAVDGVQALKILAKA
                     EKKYDLVLLDVMMPNLNGWDTLKSIRNNERTKHLPVIMLTAVNEEQKMVSGLKIGADD
                     YIVKPFVLPNLLARIEAVLRRSVWNKENTKVHELPFGSDEPVEPLTLREIEVLNLVAK
                     GSNNQAIANKLFVKEVTVKTHLNSIFKKLKVTNRTQAVLLAMQMNLIKQ"
     gene            complement(37514..38161)
                     /locus_tag="PKI94_02720"
     CDS             complement(37514..38161)
                     /locus_tag="PKI94_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW25684.1"
                     /translation="MKKNNILIVEDHELTRFGLKTTFDGQNFVKAVFEADCAEKAFEI
                     INNNNIDLIIMDLGLPNINGIEATKKIRNKNKDVKVVVLTSHNDEKEVLNSLKAGANA
                     YCSKEINPQRLLEVVQSVLEGASWFDPSIAHVVLRAAVNENFVENESKNDYNLTTREA
                     QILKLITQGLSNNQIAKELFISINTTKAHVASILQKLEVDDRLQAALKALKYKIV"
     gene            complement(38172..39767)
                     /locus_tag="PKI94_02725"
     CDS             complement(38172..39767)
                     /locus_tag="PKI94_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25685.1"
                     /translation="MAYSKFKLISLIILFGAVYFSQKVLAVDFSPINSFYPKPSAFEK
                     TINLQTQKGLVRTIDLSDTYDFSNATDRFVQCNIAASKQDFRNIIQKADKNDFFYLTM
                     ADKMAGLGFFDLSDLAISKISSDFSKNSSKAIKRFYYPSKKLTEEQEILLAESYSNII
                     YNNQSTETVKELSKNSSLLLSSDYANYIMALGYYKTGAFAKANKYIDSAISKSPSTLN
                     YQKLKVEILAETGRQEAALRIIASLKKQNLLSYEYERKINALEQYTLYKTGKTSSQRN
                     YHLGYYYYLENDSSKAIKTLQTALSGKKGVDTQAIYSLMAKIYFETSDFEKASENVQK
                     AYKIDKNNPQTLLILGDLNCNQGNFQQALKYYKQAASKNKKDYLPQIKEGIAWQKLSN
                     DQKAKEIWEKTIKENPKSWQAYYYLGLLNENNSEEYFKQSLSINPMFKQAWIELAKGS
                     ISRQNYDDAKKYLSNAFCIDENDFRYYYYQGLVEKNTGNGAQAEYNFKKCLKLKSDFE
                     PAKNELMHTEVEPNPITPVSQIK"
     gene            complement(39767..41515)
                     /locus_tag="PKI94_02730"
     CDS             complement(39767..41515)
                     /locus_tag="PKI94_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNW25686.1"
                     /translation="MVVENIKNEHIKQGEFLIRKALDEFLNKSYKEAFSAALSAQKLF
                     IQEKSSENISICLSLLGVIEYLGDKSKYLSALTYLNDGKYLAECTKNTTAKLINEFML
                     GNLDFIEKNSCTAIIHYENALKIAKRQDEYGLIEHINSRMKQLNSSCDFSTPEKHDPL
                     VALLRIGQTVSAETDINTLLEVIAQETKSAIQADRCSVFLYDRENNELWSKVALGMES
                     EEIRFPADKGLAGHVVQTGETINIKDAYSDDRFNKEIDLKTGYVTKTILCMPIKNLNQ
                     EIIGAFQVLNKNGGEFKEEDEDLLVAIGSSAGIALENAQLFKKQGEMLEEQKQVFDSF
                     MDTLAASIDARDKITAGHSTRVRLYSSLIAKAMNMDEKNVEIIEKAATLHDIGKIGIR
                     DSVLQKEGKLTDEEYKHIQEHAEITHNILEKIHMSDEFKLVTEIACSHHEKFNGNGYY
                     RHLSGEDIPLGGRVLAVSDVFDAITSKRHYRDKMPIMNVIEILLKDSGTHFDSQIIQC
                     FLSIPTNKIVEVFMTENHLTFEEEHKKILEKYSMMDLYNLSTQKQPEELSEDEKIFID
                     LFNFYYTAKSSKDDQN"
     gene            41622..42311
                     /locus_tag="PKI94_02735"
     CDS             41622..42311
                     /locus_tag="PKI94_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW25687.1"
                     /translation="MQTITILVPIFNEVNTLRIVLQKLEEVDFFGLKKEIILVDDCST
                     DGTRDILKQLQDYKIFYHEKNMGKGAALRTGFENATGDIIIIQDADLEYDPIDYQPLI
                     KLILEEKADVAYGSRLSGARPARSFMFTHLLGNKFLTLLTNILYNTTLTDMETCYKAF
                     RTDFIKGIEIKSDRFDFEPEITAKVLKKGARLYELPISYYGREYSEGKKITWKDGFHA
                     IWALIKFRFVD"
BASE COUNT        13544 a         7890 c         7250 g        13599 t
ORIGIN      
        1 gtttttgttt cttttatgtt ttgctgctcg accatatcag gcacggtaag atttaagccc
       61 tgctggtcta atagcggggc tataaccatc attatgatta aaagcaccaa aaatatgtcc
      121 gtaagcggtg ttatgtttat ttcattaaaa ctatctctgt gtcttgttgc cattttattt
      181 tatccttttt aatcttccca attctgtgta tcatttacct gttcaggctg tttttcaata
      241 ggtttaattc ttttattatt tccatcggaa agagtttttt ggtctttttt gcttaaaggc
      301 tcgccggcaa gaaccaactt tgtaaattct gcttcttttg cggcattcat aattttcatt
      361 atttcagagc ttttggtttt ttcgtctgct tttacgacaa cctctttctt ttcaagcgaa
      421 tcttttattg caagaagctc gtttgtgaga ttatcggcgc ttatttgctt gccgttaata
      481 tacataaccc cttcttttgt aaccgcaaca gttgcatctt tttgttcaac agaaactccg
      541 ctgttaattt ctggaatatt tatcgaatta tccatggatt ggaatgtcgg tgcgacaacc
      601 atcatgataa ttaaaagcac gagaaatata tccgtaagcg gagttatatt tatttctgta
      661 aacagtgcat ctttgccttt tgttgtcttg attgccattg gttttgtgtc ctttgtttta
      721 tttatcagct ttatgatttg gtgaagggtg aagcgtgagg ggtgaaggga ttttttctct
      781 tcacacttca ctcatcactc ttcacttata aagctatatt tgacgctgat tgaactttaa
      841 cttcttcctc agaatcgacg aaactcaaga aaagaagttt gattaactga aaatcatctt
      901 caaatctttt tactattgat tggaaagagt tatagaaaat aaccgcaata atcgcaacac
      961 ctaaaccgaa ggcagtagcg ataagggctg agcctatacc cattgcaaca gcggctgact
     1021 gattaccttg agttgcaagg tcttggaagg tataaagaat tctgacaaca gtaccgaaca
     1081 agcctaaaaa cggagccaca ccgccgattg tacccaaaat ggtaaggtgg tgttcaagtt
     1141 ttcttgtagc aagacttgct gctatatcaa aagaacgaga aaatcctttt ctttgctcgg
     1201 aaaaaattct caccgcctct tcgctcactt ctccgataac cccgcctaat tgaacgctca
     1261 gggtctgtgc gctgtcaagg ttatttcttg ccaattcttt ttgtaacttc ggaatgaact
     1321 gcacaacgtc tcttttgttt ttgttgtaaa aagagtatct gttgagtgcc acagcaacga
     1381 ctaaaatcga gcatactaaa atcggtaaca gtacgggcca gtccattttg attgaatgta
     1441 gtaataattc catagtttta tcctcttttt tatgtaataa cttattttct gattaataat
     1501 ttgtcgcccc aaaaacatta tagtcaaatg tgaattgaat gtctatgctg cttcctttat
     1561 aatccggcgg aagcggcttg aaaggtgccg taagttcaac cgcactgagt gcagccttat
     1621 cggcacttgg aacccctgaa gatttgaata cttttattga aagaagtctt ccgtcacggg
     1681 caattttaaa caaaagaatt acacgcttgc tttcgttgcc ttttggcgga tcccaattca
     1741 ttttaattct tctttgaagc tccctcatat aaggtccaaa atcaggttct ctgattgcat
     1801 ctatgcctgg tgctccaccg ccacctccgg gattaccgta attcccgtaa ccggatgaac
     1861 ctttagacat ttttgaaccg cttgagccgg cacttgaacc gctgccgcct gaaggtgaca
     1921 aagaaggcac gggtgcatag cttcttccgc ttccgctaga gcctgttttt gcgcttccgc
     1981 ttccgcctat tggtcctgtg gaatatgttt ttcctgtact tgagcttgat gctttaggta
     2041 taggcactgc aaattcagga tttggcttaa ccgaaggcct tggcacagaa ggaggtttta
     2101 ttgaaggtct tgccgatggc ggctgaggtc ttgcttgagg ttgtttttgg gttaactgtt
     2161 caaaaacatt tttcttttga ggcacttttt gtgcctgagg ctttttaaca gaaggttttt
     2221 ttgcactttg actagatctt gactgctgtt gtactgcttt ttttgtgggg gaaggcgaag
     2281 gcatggaaac ctttttttta ggatctcttt taccgcctgt tcttgaattt ctatctgcgc
     2341 gataaggagt ttttttattt aaaggcattt gttctttgtc aacgagcaca aattctatat
     2401 cgttcatttt tggttttggt ttttcaaata tagaaagcgt tatacccata agcgtcaaaa
     2461 ttaaaattat aagccaaaca agccccacca ccgcagggtg caaaacagtt gacaatacaa
     2521 gagatttttt aaaagaaata ccctcctgcg agtgttttag caccgaagga atggtataac
     2581 tttctttttc ttttatatcg tcatcatcta ttaaactgtt aatgtttccc aaaattgcca
     2641 ttaattgttc ctgtttgctt tgtgcttata ttttaatatt taagtgaata gtaaatagta
     2701 aatagtaaat ggatttttct gttttttttc aaattttact tttaatttct attcactatt
     2761 tcctattaac tatttactac tttataaaga atatgaacaa agattattag ccgatttact
     2821 aataatcgta gttatatcct gaagggttaa ctgtaatagg ttggtcaatc ataagctgaa
     2881 ctgttgcatt tgaagggatt ataacatcct gacctttgtc atatattgat tttgcaagtc
     2941 ctgcacctgc accgacacct gtcataattg ccgttccttt gcctacggca ccacctgcaa
     3001 tagctgatgc tacaagtcct gtaagtgcac ctgcggcaga acctacagcc atatcttttg
     3061 catattcttt agctgtatcc atttttgttc cgccttttaa aagccctgtt ccgtcatctg
     3121 tttttatcat tgcagatata ggaattctag ccccgtaagg tgttgttact tcggtaaacc
     3181 tgagcctcaa agatccgttt attgttccgt gttttgcttt tgtaacttga gttacggtac
     3241 ccataacagt acttcctgca ggtgcgatta atgttgaatt ataataaaaa tcagaaccca
     3301 aagttacact caccggttgc ccaagagtta aaaactcaga gcttatcgga gttgaaacaa
     3361 cagccgacat tgtaattccg ggcggaaccg ttacaactct gccctttagc ggctgatttc
     3421 ctgtttgcct tacctcactt ggataatagt tttgttgata ctgaggttga ggaaaatttg
     3481 gctgatattg atagttactt gccgcaaaag acaagtttgc gcctgccatt gaagccaaca
     3541 aaattaagct aaaagttttt ttgttcatac tagtgtcctc cttttaaaat ttcttgtgtc
     3601 tatatactaa taattattat tctaaaaccc gtttttgtca atttgtaaca ccttgtgtaa
     3661 taaaaacagg ggctgcaagc acgattataa gatcggcacc tgaagctaca acggtatgct
     3721 cacccatttg ccttgtggca ccgggcacat actgaagtgt gccgtgagaa aatccctgtt
     3781 ggaaatgggg ttttttatcg taagtcgcag gattcgtaag ctctcctcct atcaaattcc
     3841 ctttgggagt atatatatat gctcttatgg gtgtttcata tccttgcgga gttttaaatt
     3901 ttataacctt aataaccatg gatccgtttg tccccaccac cggctcattt attttctcaa
     3961 catacccgaa aaattcagta ttttgtggga ttacatttgt atcatacaaa tacaaatcgg
     4021 acactgcaat aaatttaacc ttcgttcctt catcgcagta ttgtgttgaa atctcctgtg
     4081 tgcttattac agggataaat gttcctttag gaatttcagc accctcatag cctctaagct
     4141 ggatttcgca cagacaaggc agagcaaaag aaatcattaa aaataaaagt aaaaataacc
     4201 ttttcatgat taataattat aacaagtaaa tagtaaatgg taaatagaag tgactaaaaa
     4261 atttagcagc tcgacagctc aacagttcag agacttgaca atacaaaatt tataaactta
     4321 tcgactattc gactttttaa ctaaaaaatc tttcagcccc tcacctattc agcctttaat
     4381 acagttcaat gtctttaata tatctgggcc tggctttgac ttctctgtaa acctgcccca
     4441 aatattcgcc tataattccc agacaaaaga cctgaatacc tccaaagaaa gtcaggaaaa
     4501 caacctgagt tgcccaaccg tttggagtcg agtgcagaac tattttttca tacagtacct
     4561 gaagtcccag tccgaagctg aaaagtgcca taaaaaaccc taaaacagca ataattctaa
     4621 gaggaattat actgaatgaa gttataccgt ttagtgcaag tcccaaaagg cttataaagt
     4681 taaactttga tttacctaac attctgggtt tcacatcaaa atttacataa gcggttttta
     4741 agcctatttc gtggaaaaat cctctcaaga aaagctgttt ttcggtatat cttgcgagaa
     4801 tatcaagcgc gcgtttgctt acaagcctgt aatcagagtg attaacaggg atttttgcac
     4861 ccaaaagatt cattaatttg taaaaaaaca atgccgtata ttttttataa aaagaatcgg
     4921 tgtcgcggcg atttcttata ccggaaacta tatcgtagcc ttgcgagtat ttatcaacga
     4981 atttttctat cgcccactgg tcttgctgaa ggtcggcatc tatcgaaaca acacaatcac
     5041 aacccaactc tcttgcccct aaaagccctg ccataagtgc tttttggtta ccgtaatttt
     5101 ttacaaacct tacccctttt acaagctgat tttttctgtg taattcttct ataatctccc
     5161 aagttctgtc ttttgagcca tcatctataa gataaacata gctgtcttca agaattttac
     5221 ctttttcgat aagggcatta atcacctcaa aaagactgct cacagtgctt tctactaaaa
     5281 gttcttcgtt aaaacaaggt acgataattg ctaaggtcgg tttgcgcatc ttaaaaattc
     5341 ctattatgtt ataatattca tattataata tttctgggtt cagagcaagg ggtttttatt
     5401 ttgagtaaca aaaaatttat acttaacgca gacgatttcg ggatgtcaaa cgcttttaac
     5461 agagccgttt tggatggtta taaccacgga tttcttacaa gcgcaagtat ctgcgcaaac
     5521 ggtgaggcat ttgatgccgc ggtaaacgaa attattcccg aatgcccaaa tttaggaata
     5581 ggggttcatc ttaacataat cgaaggctat tccctgagta attttacaaa aatgaatatg
     5641 ctctgcgacc tgaacagtaa atttgtcaaa agctacggac aaatgattta tctttcaggc
     5701 aataaagatt ttatggcgca ggttgaaact gaatttcgcc tgcaaattga aagggtttta
     5761 aattatacaa aaattgacca tttggactct catgtccatg ttcatgcaat tcctaaaata
     5821 tttgaactga cctgcagact tgccaaagaa tacgaaatta atcaggtaag aactcaattt
     5881 gaaaaatttt atacggtacc cgatataaaa tttcacctaa atttaaaata cccgccaaat
     5941 cttttaaaaa taatgctttt aaactatttt accctaataa acagacaaaa aataaaggaa
     6001 tatgggcttc aaactaacga ctatttaata ggagtgggct acacagggat gatgaatgac
     6061 aaaacccttt actgcggact tgaaacgata aaccaagatt gtaccgtcga agccttaatc
     6121 cacccttgca atcacataaa ctctaaaaaa gcaaaatctc aacaccagat tgaatttaac
     6181 ataactcaaa atatggattt aaaagacaaa attcaacgtt tagactttga aataaccaac
     6241 tacaagagtt gaataggtta ataggtgaat aggtgaaagg ttttactgtt actattttaa
     6301 ttttttcttt tcttgtgttt ttggcttacc agtttcaaaa aacagccgaa tttgatgtgc
     6361 ttgaaattac ctctccgatt gaatttacag ttgatttaaa caacaacggg ataaaagatg
     6421 atgacgaaga aatttttctc gaggacataa aaagtttttc gacaaaaccg agccaaagcc
     6481 aaacagagct ggcaaaaaaa atagggattt cacaagaaga tgctttaggt ttgggatttt
     6541 tggcacaaaa ttttgcaaaa gaaaatttaa gcaaaaaagt taaatttaca ggaaaagaag
     6601 ataaaaaaat cctcattgac aataaagttt atgaaaaaat gcttctagaa caaggctttg
     6661 ccacctccaa aagctatgca aacaaagaca aaatccaaca aaacatctta aaagcaaaga
     6721 aattaaacct gagaattttt aataataaaa gtttaaaata tcacaaatta aactgcaaat
     6781 acggacttct ggctcataat tcgcagattt tgcctcttaa tcaaattccc aaagacgcaa
     6841 aagcctgtca attttgctcg ggcaaacaaa aagagaaaaa taaatcaaaa aaaattataa
     6901 caatatatga agaaaaaatt ccttatgcac caaaagcagc gggtattttt aacggcaggg
     6961 gaattgaaat ttatctgccc gatttaacct cagttctaaa accttcaaac aagtgtaata
     7021 cggatttttg cagagcactt ctgagagaaa ttaactgtgc gcaatcgtcg atatattttg
     7081 caatttatgg ctacacaaaa actcccgcaa ttcaaagtgc gctagaaaac gcgcaaaaaa
     7141 gaggggtaaa ggttcgcttt gtttatgata tcgacggcaa aaaccaaaac ctgtatcccg
     7201 acacacttta tctgacaagt gtttttaaaa acaacaatgc tgattttgcc gtagaaaaaa
     7261 aataccaaaa cataattatg cacaacaaat tttttatctt tgacaacaaa aaagtactga
     7321 cgggctctgc caatataagc gatactgatt tatcggggtt taattcaaac gctattataa
     7381 tcctcgattc acagcaggct gcaaatgttt actcacagga atttgaacaa atgtatggcg
     7441 gtaaatttca caataccaaa caaaaaatct gcaacaaaga aggacttcaa gtaggcgaca
     7501 cagaattttc tgtctatttt tctccgactg acaggacaat gaattcaaaa ataattccgc
     7561 tcattgacag tgcccaaaaa tatatttaca tgcctgtttt tctgattact catcagggtt
     7621 tatgcgacag tttaataaga gcaaaaaacc gcggagtcga tgttaaagtc ataattgatg
     7681 ccacaaatgc ccacgggact tattcgggac acaaactttt aaggcaggca ggaattaagg
     7741 tcaagacaga aaattatgcg gggaaaattc attcaaaatc aataataata gatgatttat
     7801 acacaattat aggctctatg aatttttcac gaagcggcga taataaaaac gatgaaaatg
     7861 ttttggttat aaaaaatccg gatattgcag tgttttataa aaagtttttt caatatttgt
     7921 ggctgagaat tgacgacaaa tggctttaca aaaacgcgcg cgcggaatcg cccgattcaa
     7981 taggctcatg cacagacggc gttgataacg actttgacaa aaaaatcgat atgcaagacg
     8041 aaagctgcag ggcttattaa ataataattt tatcattatt atgttttgcg ttgttttaca
     8101 tataataaca agttgcatat tatatgtaaa tgtgttaaaa taataataat taaaagtata
     8161 ttatgaaagg tcgttaaaaa tgaataacta tgaagcagga tactatataa aagaaaaaga
     8221 ttttagagcc tttgtacctt accctataaa caagccgttt gaaatatatg atttaaaaat
     8281 aatttcttta ctggaaaaag cgggacgatt gataggtgaa ttaaatgcat attcaaaaaa
     8341 tttacctaac atccagtctt tcttgtggtt tttgagttta accgaggcaa actcttcaaa
     8401 taaaatagag gggacaaaaa taactttaaa cgagatcata tcacctgaag aagatatttt
     8461 gcctgaatta agagatgaaa aaatagaaat ccttaattat ttggtttcat tggagaagtc
     8521 ttacgaaaga ataaccggta cagcaggtat tttgactaca cctaaaaaat caaatgtaga
     8581 acttttacct ttttgtacaa gattgattaa agaagcccat aaaatattgc tcaccaattc
     8641 acggggcaaa aataaaaatc ctggaaagtt cagaacttcc caaaactgga ttgggggaac
     8701 aatgccttca accgcctctt ttgttccacc gccttgtgac aaaatccctg aacttttatc
     8761 cgatttcgaa aatttttggc ataatgacga tttaaatatc cccattctaa ttaaaattgc
     8821 gatttcgcat tatcaatttg aaactattca tcctttttta gacggtaacg gaaggatagg
     8881 aagattaatt atacttatgc aactggttga cagcggaatt ttagaacttc caaccctata
     8941 tctttcaaaa tactttgaaa aaaacagaaa gcaatactat gatgccttaa atttagttag
     9001 agaaaaaaat gatataaatc aatgggtaaa atatttcctg gaaggggttt gtgacatttc
     9061 caaatataca gttattaaat taaaagaagt aaatgattta aacaatctat gcgaagaaaa
     9121 aattaaaaaa ttgaccaaat cacatgtaaa tgcaagccga ctgcttaagc ttttgtataa
     9181 aaatttatct gtaaaagtaa atgatgtcgc acgcgaactt aatttaaatt atcaggttgc
     9241 aaacgcctta gttaaggcat ttgtaaaaat ggggatacta cacaaaggta aacaaaaacg
     9301 taatacttca tatattttca gagactattt taatttattt gttgattaat taaatcccag
     9361 cgtaagttgt ttgtcctgag tttgcctctt ataaatttca aaatcaaaac atccgatgtc
     9421 agaaattgca ggcgaggctg catcaagccc ttcaagacgt gattttccaa ctttgcaaag
     9481 cggtttatat tcacagtgaa aacatgtttg aaattgcttt ttcatttttg tacccctcac
     9541 ccggatttct aacactcgtg cctcgtgttg aaattccacc ctctcccgca aggggcgagg
     9601 gaaaatatta ttaaaattta tcccctattt accatttact attcactcct ttaggcatga
     9661 gcatacaggc agcatttttt gtatttttta ccgcttccgc aaggacaagg ctcatttctg
     9721 cctacatttt gcgcttcgat tttgggcttc tgctcttctt cttcgatata acccaaagtt
     9781 tttgcaaaaa ttttagactc gtataaaacc gttgtcgaag aaaaaatctt ttccgttaag
     9841 tgctgttttt tgtatttttc caataactcg tcaaatgtca tttctgtcga ataaattttg
     9901 tttatgacct tcataaaatt tttgtatttt gcttcaacgc gccttaaaag attagctgaa
     9961 acgctgtcgg ttaaaagaaa atgttcgaca catttttcgt aattttcaat tgttttataa
    10021 tctttttctt caaaaatttt gcaaaaagtt tggtaaaaag gaattgcaga aagtcctaat
    10081 tcctcatcaa aaattactcc cacatcataa gtttctttgt gcgccgaaaa ccctcccaaa
    10141 gagttttcca gaaagttttc gtaagagttg ttaaattcct gcacatggaa aaatttgtgc
    10201 tcatcaactg tttttatttt tgattttatt tcttctgcca aaggcttttt tgcgccttct
    10261 gcaaaatcgt tgaaaagccc tataatatca tctgcaagtt tatttgttgt aacaatttct
    10321 tctttgccga aacactccaa aaaagcttta tggagttttt tgacctgaac ttcgatttct
    10381 ttttgctttt tggggttgtc tttatagaca atctcgggcg attggataat ttttgcaatg
    10441 gcaaatctca tcgcatcttc tttttggttt gccgcaagaa cattggagat ttcaagcagg
    10501 taatattcgc cttcaaattc aaaaatcctt gcaacaacgt actgccccac acccacaccc
    10561 ctgaaatgag tcattttcac aagcgaaatg acactatagt ctttttcgtt taccagattg
    10621 taaaattcaa acccgttttt gagaattttt ttcacttcaa aaacagaata aatcgaaccc
    10681 gtaagtgctt ttaatataag tcttgcgcct ttggtaattt tttttgtttt ttcaagaaaa
    10741 agttcaataa tgctttttga gtcagagcct aaatttcttt caaaaatata cggcagacaa
    10801 acggattgaa gctgattttc agaaagtgtt ttcaccccga ctgttgacaa atattcggta
    10861 aaatcggctt ttacctcatc atctgtttgc acaaaatcaa ttaatccgct catcacgtcg
    10921 cttatatcgg aaaatttttc tattacgctc atttctctcc cctttttaac atcaccgcca
    10981 aagaatgtct tgcacagttt cctttttctt ttctgtatga aaaaaataac tcatttttac
    11041 agcaagtgca atcctcacat aaatctattt tttctacccc catggctaat agctgaaatt
    11101 tattcacaag ctttaaatcc acattcattt tatcacacac aagcactttt tcttcatcct
    11161 taacgcttga aagaattttt tccatagtgt ctttatcaac ttcaaaacag cattttccga
    11221 ttgcaggacc tataagtgcg attatgtctt cagattcgct tttaaattct tcttgcattt
    11281 ttttgactgc tttttgaact atttttgctg ctgtaccacg ccaccctgcg tgaatnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacatt atcgctgtgg gtttgatttg
    11461 gaattattaa attttctaaa gggattttta acccttcgca gacaagtttt ttgttttgtt
    11521 cgatttgacc ttttaagtct tgtctttcgc cacacttcaa aggcagttcg cgcgtggtaa
    11581 aaaaagcaat aacaggctca tcttctaaaa aagatgattt caaaaccctt ctgcccataa
    11641 attcttcttg cttaaacata agtctattat aatatgcttt ttttatagta caatatatta
    11701 acaaggcaaa agagatgcga gcggacaagg ggtttttacc ggcgaggttc aacgcaaaaa
    11761 tatagcgagt gcatcaatcg ggtaagccga caaaacaaaa aataggcaaa tacaaaataa
    11821 aacaatgaaa ggcgcgtaaa aaagatttcg tgtagtggta aaatgtttga cagtttatcg
    11881 gaaaaattac aggaaataat cagaaagact tctcagggga ctttgaccga agataacatg
    11941 ggagaggctc taagagaaat tcgccgtgcg ctgttagagg ctgacgttaa ccttcgggtg
    12001 gtaaaagcat ttatcacaaa cataaaagaa aaggctgaag gcgaagaaat cttaaaagga
    12061 attaatcctt ctcagcagct tgtaaaaata gtccacgacg aacttgtaga tgttttgggg
    12121 caccaagttt cgccccttaa tttaagcgga catccttcga tagttatgat gctggggctt
    12181 caaggctcag gtaaaacgac atcctcggct aagcttgcgg ttaaacttaa aaaagagggt
    12241 aaaaatccgc ttcttgtggc atgcgatgtt tatagaccgg ctgcgataaa acaaatccaa
    12301 actctggggc agcaaatcgg ggtggaagtt ttttcagatg aaaactcaaa agatgttcaa
    12361 aaaattgttc aaaactcgat taattacgca aaagaaaacg gatttaacgt tctgattttg
    12421 gacaccgcag ggcgtctgca gatagacact gacatgatgg cagaactttt gcttatagat
    12481 agagcctttg tgccacacga aaaacttctt gtgatagatg cgatgacagg tcaggaagcg
    12541 gttaatgttg cccaaaattt tgacgcacag cttaatctga cagggcttgt tttgacaaaa
    12601 ttggacgggg attcaagagg cggctcggca ctttctgttg tctattgcac caaaaagccg
    12661 ataaaactga caggaacagg ggaaaaatta aacgcgttgg aagattttta tccagagcgg
    12721 atgacaacaa gaattttggg aatgggtgat atcgtttctc ttgttgagcg cgcgcaggaa
    12781 gtttttgacg aaaaagaagc acttgaactc gagaaaaaaa tgcgcaaagc cgaattttct
    12841 tttgatgatt ttctaaaaat gcaaaagcag atgaaaatgt ttggctcatt agaccaactt
    12901 ttgggaatgc ttcctattcc gaatttgaaa aaagaagaca aagaaatgat ttctcatcaa
    12961 ggcgaaaaac agtttaagaa aatagaagtt tttattcaaa gtatgacgcc acaagagagg
    13021 gctaatccga atttaataaa ttccgacaga aaaaaaagaa tagccgcagg aagcggaatt
    13081 tctttgcatg atataaatat tttcataaaa cagtttgagc agatgcgcgc gatgatgaag
    13141 ggcttttctg acgtcaaaga caaaatgaaa ggcggaaaaa tgaaccccgc tcagatgatg
    13201 tcaatgatga aacggtttaa atagtttcag cttttctcaa aacagatgaa gcacaagcag
    13261 taagtgtcca gaaaacaaac tggatttgag ggcggaaaaa taccgtgtca acaagcccgt
    13321 gtaccataac ggcaaaaacg cttataagcg aaattgaaac aataaggcac aatttaaagt
    13381 cgctgttttg tgttataaat ttaaaagtct gctttgcaaa aactcccaaa aagcccaaaa
    13441 atgcaatcag agcaaaaatt ccgctctcga ctgctacttc caaaaacacg ctataacaac
    13501 tcaaagcgtc aaaacctgtt ttcatataaa gcccataaat ttctctgaaa ttctgatttc
    13561 ccaccccgat tccaaaaagc cagttgtctt ttatcatctc aagtgcggaa tgataaacgt
    13621 tcaatctgaa agaagtagaa gaatccgccc gcatcgcaaa aatggacaaa acccttgtcc
    13681 ttacagaaga aataaatacg accgcaaatg ccacaagcgc tgcggcaaaa gccgcaagtg
    13741 aaacataaat tttcttaaaa aattccctgt ttttttccaa atcgctccag ataaacttgg
    13801 caagaaaagc aaaaatcccc acaataatcg ccaaaaatcc caaatacgct cccctgcttc
    13861 cagataaaat caaagcgtaa gaagaaacca aagcaagcac aagaaaaaat attcccaatt
    13921 tgtaatgttt ttcataaaat ctcatcgcgg aaaacccaaa caagcaaggc aaaacagcaa
    13981 ccaaataccc tccgaaaaga ttcgggttaa atggctgaag agttccgtaa acccttgtca
    14041 tcacatcttc ggggttaatg tttgtaacat cctgccaggt agcgatttct cccacctgcc
    14101 cgaaattctg gaatatcccc acaaagcctt caatacccgc acaaagtgcg attaaaccta
    14161 ttgcaaaagg aattttggaa agattgtttt taaaatattg aacggcagaa aaataaaaac
    14221 ccaaatacac aaatgttttt aaaaaacctt ttaaacttaa atgaaaaagc gttgagcccg
    14281 aaagacttat aactacaaaa ataaaataaa caagcaaaag aagttcaaat aaattccctt
    14341 caattttttc gcctttttta acaaaaagtt ttatgacagt caaaaacaaa gtgattaaag
    14401 cgacatagcc caaagcatca gagggcataa atatagaaag ccccaaaacc gcaagaattg
    14461 atgcaaaaat caacccgtca atgtgtttta taaaaagact gttttggctc aagtataaag
    14521 ccttttcttg cgtaaaattt aaaacattgt ttatcgaatt tattattaaa ccttcaaaca
    14581 tacttatctc attaaattgt tttggattgc cgcgccggct atgccggctc gcaatgacat
    14641 ccttctacgg ctcgcttggg ctatcgcctt gaattatgtc attttttctc ttgaaccgga
    14701 gcaagttgtg gattttgctt tgcctgtgcc atttttgcct gataatcttt caagactttt
    14761 ttaatctgtt cgacttgctc ttttgataaa atttgaacat ttgaaacaag tccgttgaat
    14821 ttataatctt taccttcaag cactatggga agacctattt taactttatt tccacctaca
    14881 accgcaccat catcggtaat tttggcatcg tcgataaccg ttaccacaac atcatacata
    14941 aacggctgcg aaagatcttc aaccattaca aaaggctctt ttgtccttgt gcttggcata
    15001 attgtttttt tagaatctgc cttaacatct ataatctgca actcagtata aggaacattt
    15061 cttatgctga taaaagtttt gtcgccaaag cgtatgggac tctgtcctgt cgtcaaggta
    15121 atacctctta aaaaaacctg aaaagcaatt ttggcttcac cttcaatctt atttgaagcc
    15181 gtctgcctta cgtttttata agtcaaaaat cccacaacaa gggcgataaa aacgcccaaa
    15241 attactataa tgtctattaa tttgagtttt ttgttctcca taaaatcctc ttttctacaa
    15301 tgcaattttt tctaaatttt ctaaaatttc ttctactgtg gtggtagcac atccgaaatg
    15361 tcttaccacg atacttgctg ctatgtttcc tatgattgca gcatacttgg gttgtgctcc
    15421 gcttgcaagt gccatggtga atatagccgt gacggtatct ccggcacctg taacatcaaa
    15481 aacttccgac ttattgaaaa caggtatttt tgtcatttcc ccattttcgt taaaaaccac
    15541 cattccttca gaaccgcaag ttattaaaac ggattttgcg cccgatactt ccaaaagctt
    15601 tctgcctgct ttttcaagta acacatcgtc tttaacttca aagccgacaa atttttgagt
    15661 gtccggaaga ttcggcgtca ttgaagtcac gtctttgtaa ttttccaaat tcttttgagc
    15721 gtctgctact ataattttgt tgtgtttatt tgcaatttca atggcttttt ctatcacctt
    15781 ttgagttaaa gacccgatgt gataatccga aataataatc gcatcgtatt caggaatagc
    15841 gtgctcaaga ttatgaataa cttttttttc ggtgtcctca ctcaaaaaag tgtttgtttg
    15901 tctgtcaact cttactatct gctgagtaat cgactgtgaa caagagcctg aaatccttgt
    15961 ttttgtcgtg gtttttctgt ttttatcttt aacaagatat tttgtatcga tttttgcact
    16021 gtggaacgca caaattaatt gttctgcctg ataatcttcg cccaaaacgc ctataactcc
    16081 gactttgccg ccgtttatta cagaagcgtt gttagccgca tttgacgcac cgcctaaaat
    16141 taatttggtg tgggtgtgct gcaaaatcag aacaggtgct tctcttgaaa ttctttcggt
    16201 gtctccgtaa atcatttcgt caagggctaa atctcctacg acaaggactt tcggtaatct
    16261 taaatttttt atacattcaa tcagtttgtt tttatcaaca tccatttttc acatcctgct
    16321 tttgcttgat aattcgaaaa aattaaggta taataatttt aagtttacca taaataaaaa
    16381 tgcgaggtca attttgagca atccggaaga agaaaaagag ctgggggtaa aaacagagtc
    16441 tgtcttaaca caagaaggca atgatataga atttgaagac acaattgaca tagataaaat
    16501 tcaacaaagc ctacaaaaac atctggaaga agataaaaaa aacgaagaag tcgaacaaga
    16561 tattattcaa gaacaagaat tcgctgacga agaaatccaa caaacaacag aaaaagcaga
    16621 acccgaaata cctgcagaaa ttgtttcttt gcaatccgaa caagaactgc ctaaactttt
    16681 acaaaatgca actcctgaaa ttgaccctaa tgcaaaaaaa tatgtcatat acatagaccc
    16741 tgaaaatatt ccttttatgg aaaatctttc cgtcagcgaa agaaaagaca taataaacac
    16801 cattttaaaa gaacaggaag aaacagtaag aaagcaaaaa gctataaatc aaagaaaagt
    16861 tttcatgcgt catataattt ttgcaagtat cgtgtttata ttcggttttc ctattttatt
    16921 ttttgttgta aataaatcaa tggaagccgt aatgtcaaat tatcaacagg cagaaaagaa
    16981 cttcagcact ctttataaac aaaaaggcaa agtacaacca ttttctccta aagctgccga
    17041 aaaattcaaa tactaaaatt tcttaacatt cggagtatat actctttatt tttaattttg
    17101 tgaatagtac aattatagaa agtagagaga aaaaggattt ttgatgctca gattagacta
    17161 cagaaatgca agctcgggta ttataggaaa cgaacacggc cttgaaatag aaaaggaatt
    17221 tgaaaatcat aaagaaagaa tttcttcgat aatttccgat ttaaacaaaa gaaaagacaa
    17281 acccggacaa tggctgcaat ggatgaattt gggctacagc gaagagacca tttggtatgt
    17341 aaaagaatat gcttcacttg taaaagacag atttgaaaat attttagtct taggaatagg
    17401 cggctccgca ctaggcggaa ttgcactaac agaagctctt cttaaacctt attggaattt
    17461 acttgacagc gaacaaagaa ataatttccc cagaattttc tttttagata atatcgaccc
    17521 tgaccaaatt gcaggacttc ttgaaatttt ggatttgcaa aaaactctgg taaatgtaat
    17581 tacaaaatcc ggttcaaccg ccgagacaat gtcacaatat atgattgtaa aagacataat
    17641 ggagcgtgag cttggagata attacagaag aaacatcgtt gcaacaacag ataaaaacat
    17701 gggaattcta agacagcttg ccgatcagga aggttacaaa acttttatag ttcccgatga
    17761 tgtgggcgga agattttctg ttttctcggc tgtggggctt ttgccttttg cgctcgtagg
    17821 gcttgatatt gacgaaatta caaacggcgt taaagatatg gatttggcac ttaaaaatac
    17881 cgatgttttt gaaaatatcg cagcacaaaa cgctttaatc cactaccttc ttgataccca
    17941 aaaaggcaaa aatatttctg tcatgatgcc ttattcaagc agacttaaat atatagcaga
    18001 ctggtatgtt caactttggg cagaatcttt gggtaaagaa actgacagaa acggtgaaaa
    18061 aattaactgc ggtccgactc ctgtaaaagc actgggtgca acagaccagc actcgcaaat
    18121 tcagctttac aacgaaggcc ccaatgacaa agtcataaat tttgtaagag ttgaagagtt
    18181 tgataataag gtggaaattc ccaaaatatt tgaatataca ggtatcggat acttgggcgg
    18241 aaaaacaatt aatgacctga taaacgcaga agccgatgca acaaaagttg ccttaagcga
    18301 ttttcaaaga cctaacatta caatctctat ccctaaaatt aacggctatt atatagcaca
    18361 actgctttac atgtttgaag tccaaaccgc aatagcaggt gaactttata acattgatgc
    18421 tttcaatcag cccggtgtcg agcaagctaa aaattatact tatgccctta tgggaagagc
    18481 gggttacgaa gattctgccc aaactctgaa agaaaaaatg gcaataattt agttgaaaag
    18541 tcgaacagtc aaatagttta taagaagata agatgagtag atgcgtggat gcgtgggtta
    18601 tcctactacg cctcttggca tcttcgcctc tctatttacc atttactatt tactatttac
    18661 tttttttgca gtataattag caagtggtaa gtgtaaaatt gacacttttg cttcggggaa
    18721 aaaataaagg aattagaatg agcaatgaat taaaaaggac attaaccctg acagatgcta
    18781 tttcaatcgt agcaggatct atgattggat gcggaatttt tatagtgtcg gcagacattg
    18841 caagacaagt gggctctact tggcttttgc ttttggtatg ggctcttgcg ggatttgttt
    18901 ctttttgcgg tgcaatagct tacggggaac ttgcagctaa catacccgat gaaggcggac
    18961 aatacatgta tcttaaaaag atattcaacg ataaactcgc ttttttattc ggatggactt
    19021 tatttttggt aattcaagca ggctctatcg ctgcaatttg tgttgcgttt gcaaaattta
    19081 cgggaatttt aacccctttg ataagctctc atgtagcctt atttcactta ggaccgattg
    19141 ctataacttc tcaaaaactt tttgcaatgg caactgttct tttgcttacc tttgttaatt
    19201 caagagggat agaatacgga gtaattactc aaaacttgtt tacggtcaca aaaattttat
    19261 ctcttatagg aattattttg tgcggtatct ttttcggaat gaattggagt gtaatacacg
    19321 ccaattttgc aacctcaact ttcagcttcc attttagcga tatagcaaaa atttccaccg
    19381 caacagtagg tgcgcttttt gcatcaataa cctggaataa cgtaacattt attgcgggcg
    19441 aaatcaaaga acccgaaaaa aacgttaaaa aatctcttgc ttacggggta gggctggttg
    19501 ttatacttta ctttcttata aacgtaattt atgtttcttc aattccttta aatatgattc
    19561 aaaatgctcc ggaagacatt gttgcagcag caacaatggg tgcaattttt ggaagcatcg
    19621 gaaaacattt aattgcgata gtaatcatga tttctgcttt cgggtgcgcc aacggaatga
    19681 ttcttgcagg cgcaagggtt tattatcaaa tggcaaaaga cagattattt ttcagaagtc
    19741 ttgccgttat caaccgccaa acaagagtcc ctgttaattc gctttggctt caatgcgctt
    19801 ggatttgtac tcttatagtt tggggaaatt acacaaaact tttggatttt gtaatttttg
    19861 cctcactgat tttttatata atcacaatga taggaatttt tgtttacaga aaacaacacc
    19921 ccgatgaaga caaaactttg gtagttaata atttcttccc cttagccttt ataataattt
    19981 caacttacat agtaatttgt ttggctatgt acaaaccgct ttataccctt cccggatttt
    20041 tgataactct ggctggtctg ccggtttatt tcctatggaa aagggcgaag aagtaaaagt
    20101 atattaattt atttataatt ttagatgttg tttaatatta tcaacatcct gttttatttc
    20161 tctgatgtta taaatcaagt cccttattgc ctcgcacagg tcagaaccca ctgacattaa
    20221 aatgtctata tcatcaaatg ttaaagtttc tttttcttct tccatattta ttttcctttt
    20281 tgattaattt ataaaaataa tataccatat tagcacgatt gtcaatcttg gcattatcct
    20341 gttaattcct tcacagtatt agtatgaata aaaaagttaa gaacaaatta gcaaataata
    20401 ttaaaaaact cagaaaagaa aagaggattt cgcaggaatc tttgtctttg gagttggatt
    20461 ttgatgtttc ttatatcgga aaagtcgaaa atgaaaaaat gaatatttcg atagatagaa
    20521 taattaaaat tgctgatttt tttgaggtta actttgaaga tttgtttaaa taaaattttt
    20581 ctttcggtac tttttggttg gggcccgcca ttttgattaa ttatcaaaat ggaagggata
    20641 aaagtaccac aaaaagccac cccctgctgc tcatttttaa atttgagtac ggctcattac
    20701 attgaaaatc ctcaactcgc tatgctcaaa caatcggatt ttcgggcatt acatttcgcc
    20761 tactcaaact aaaagaactc gctaaagggg gaaagggggg cattgtataa cttcaaatag
    20821 ccacttattt tccgaccata tcggagtgag caattattac ttcttactca ctcgccttaa
    20881 acggcgttac gtgcgaaaaa atgtgatttt tcgcacttca gcctcggctc gttcgcgctt
    20941 aacgaaacaa ctgcaaagcg agtgtgtttg agtgccgaag gcacgagttc gcgagcttga
    21001 gttgagttta gtaataatta gcgaacgcag ataggccgtg gctttgcctt cttttcaagg
    21061 cgagattaga tcccgcaaca ggtgcgggat gacaaatcga gccgtagaaa atggcgacac
    21121 ggaacaacaa aaatattccg cttaatatgt ttgtttgcaa tagtggctat gtggtacttt
    21181 tatcccttcc cttttgatac ttaatcaaaa gggtgggccc caaccaaaaa gtaccgaaag
    21241 aaaaaagtat atttatatta ataacagctt tttttgcgga aaaattatta aaaattttcc
    21301 gctctacctg catgcgctgc gctcgcatta aacggcaacg ctctcaacaa aatcggaaag
    21361 cgtcgtttcc gattattgtt gttcgactct cacttcgttc gtgcctctgc tcgcttgcaa
    21421 aaaaaagcta tgaactttag gggtgcatag ctgttgatta gggatatgtg tatcgttgtc
    21481 tctaaggagt atcgatttaa taggtttata ttagcaattt atttctttaa ttaaatcata
    21541 catttttacg atttaattcc atataaagcc tcttcaaatc ctccaatctt atggggtaaa
    21601 taagtggacg gctaaaagat gcaaagaggc aaagacacaa agaaaagctg caaaatatag
    21661 aaaaaactat gcatctatgt atccaggcat ctttgcctct ctatttacta tttactattt
    21721 actatttacc atttactatt tactgctata atggttttat ggaaaaaaca tctttagcgc
    21781 agtttgtgca ggaaaaaaga gaaaatttgg gattatccgt aaaaggattg gcaaaaaaat
    21841 gccaccttaa tttggacttg attgaaaaaa tcgaagccgg agaagagctc tttttatcca
    21901 cgacggtaag acaaaacctt gccaaagtgc ttaagtgcga gcctgcagaa attaaagaac
    21961 ttgaaaagga ttttgaaaac aaatttgttt ctcaagaaat tattgatgaa ttaaaagaaa
    22021 acattttagc cggcaagact gatttgacat gcccaaattg cggctcaaat ttaataacaa
    22081 gaattgcaaa attatatgat ttggaagaca acctcatgct acaccctaaa gcaagatgct
    22141 caaagtgtgt ttttcaaatt aaaaattaag ggtaaatgac ttaaaaggtg ccgagtttta
    22201 aaaaactcgg caccttttat ttgatatttc ttcacatttt aagccgaagc ttttgcggct
    22261 tcaaccaaaa cactgaaagc ctctttatca ttaacagcca tatcggcaag catttttctg
    22321 ttaacgatga tgtttgcctt tttgcaggcg ttaacaaatc ttgaatagct catgccttgt
    22381 tctctgactg ctgcgttaat tcttacaatc cagagtcttc tcatgtttct tttcagaacc
    22441 cgtctgtctc tgtattggta tttaagtgct ttcataacgg cacagtttgc aactttaaat
    22501 attctgcttc ttgcgcctat aaaacctttt gccagtttaa gtattttttt atgtctttta
    22561 cgactaacgt ttccgcgttt tactctcata attcacctac cttgcatact tcttgtacgg
    22621 taactgtttt gataccaaat ccacatgtgt ttctgatatc tcttccatac cggaaattct
    22681 tctttttctg gtggaacttt tgtttgcaag aaggtgacct ataccggcgt gtcttctcaa
    22741 gattttgccg ttagcggtaa ctttatagcg tttcgctccg gacttgtgtg ttttcatttt
    22801 tggcattttc ttttctcctt tattttaaag ttaagtacaa aaaagattat ggtagccaat
    22861 accataaaac tttcggcttt gttttaccat aatatcaaat caaaactcac atgcaagcat
    22921 ttaattaagt tttataatat tcctttggta gaaggaatgg aatctttgtt tttttcatca
    22981 aaagaacacg cctgcctgag tgctcttgca aaagacttga aaacggattc tattatgtga
    23041 tgggtatctg tgccttcaag aagttttata tgaatggtgg ataaacttgc ctgagcaaaa
    23101 ctgttaaaaa agtgcggcag aagaacagtt tcaaaatcgg aagttttttc atctttgatt
    23161 tcgacatcga cattagaaaa agcccttccg ctcaaatcaa taacagcaag acaaagtgct
    23221 tcatccatag gcagaacaaa gcttccgtaa cgaactatgc cttttttatc ggcaagagct
    23281 tctttaaaag cttcacccaa ggtaagcgca acatcttcta tcacgtggtg attatctttg
    23341 tcgtgggatt taacatctat ttttaagtca aatccgccgt gtgctgcaaa ttgttcgagc
    23401 atgtgggtga aaaattttat cggcgtagaa atttccattt ttcctttacc gtcgatatta
    23461 agttcgattt taacttcggt ttcgtttgtt tttcttgttt tagtgctttt gcgcatatct
    23521 gccttctttc aaataatttt tcaaatcaac cgtctgctcc aatactacca cagctttctt
    23581 ggatttcaaa aggtttttta aatcctctga tttatcctgc ggaggcaaaa cgcctattgc
    23641 ttttacccct gctttttgcg cacaaatcat atcatctacg gtatcgccaa gataatagat
    23701 ttcatctgca ataatttttt gcttaacgat ttcaagcccc aaaggatcgg gtttttgacg
    23761 attaagcccg acatcttgca tggtgattat cggataaaaa tattttgtaa aattgttttt
    23821 atcaagcgaa taataagctt cttcgacaag ccttccggta aatactacca aattataatc
    23881 ttttgacaac tcttcaaaaa attctgcact tacaagcggt tcttcgattt gggcaagggt
    23941 tttgtaatgt ccctggaaaa cttccacgat atcatcataa tcaattttta aaccttcgct
    24001 tttgagcaaa tattctgtca ggtcccaatc gttattaagc cctccaagct tttttgtctg
    24061 cgaaattttt tcaagcgaaa attctttttt tgcaaaatgc tcaaaagttt tttgcacggc
    24121 aacccgataa gactttgagg cgtcaattaa aactccgtcc atatcaaaga ctattgcggt
    24181 ttttttttca agtaccgata aaatcttttt cgtgttttca agtgtaggaa ccgtaattct
    24241 catagtgttt tcaagcaatg gaatattttt aaaatattta actttgattt tggcatcaag
    24301 aagttttttg tagataaatt cggctttttg cccaaaatcc acgcacaaaa aatttgtctc
    24361 agatgggtag gggtaaatgg tattgtccaa tttttttaag ccttttatca acatttcctt
    24421 tgacacttcg atttcttttt taaccttttt aaaatattga atatcgtcaa gtgctgcaag
    24481 tcctgcttta acagacaaat tcgaaacatt gtaagggctt aaatattttc tcaaattaga
    24541 aatgttttcc tcagatgaaa ttacatatcc taaacgcaat ccagcgagtg cgaaatcttt
    24601 tgaaaaagag cgcacaataa aaatattttc gtttttcctt gccaaatcaa gattcgaaat
    24661 ttcgcagaaa ttagcgtaag tttcgtcaat caaaattgct tttccatttg cttttttgat
    24721 gattttttct ataacttttc tgtcgattat ttcgcctgtt ggagaattcg gtgttgtgag
    24781 gtgaatcaag tcggcattgt caatattttt caaaaattct tcttgcggaa attcccactt
    24841 ttttttgtaa gaaatttctt tgaagttaag tccgttaact tttgcataaa tgcggggcat
    24901 aacaaaagaa ggctcaaccg tcaaaaccgt ttgccctgat tccaaaaaag tcgaaaaaat
    24961 tgaacttatg gcttcatccg cgcctgcggt caaaattata ttttcaatac ctaccccgtg
    25021 aaaatcggca agtttttgaa tcaatttgcc gtaataaggg taatatttaa tatcttgggg
    25081 cgaaatattt ttaatcgcat caacgacttt tggcgaaggc ccaaaatcat tttcgttgct
    25141 atccagcttt aaaaaacctt cttcatcaaa aaggtcaacc tgatagggct caattttttg
    25201 aatagttttc ttggctttca ttcgacaatt atataccaaa aactactttt ttgaataaag
    25261 cgataaaaga tttgttcttc cggaagaaga attgtcctga tagctgagtt tgttgtttaa
    25321 gggttcaatg ttaacttttg gggcatcttt tttcttaaat atattttttt ctgcccaacc
    25381 tgcaagagga gttgaaagga aaggaacaat cattcttttt gtgataattt gagttgcaac
    25441 aagcatgctc accgcgctaa atccaacctt aagccatttt gcggtttttc cgcctttgcc
    25501 gagcatttta tttttaagga aattttctat ttttactgcc gtaatttctt tattgtttat
    25561 tatttcttcc aaagtttttg caatttgtct tgttttattt gttcctagtt ttttctccat
    25621 taaattatca aaaaatttag gagcagcctt atctaaccat gagccaactg taaattgaag
    25681 caagacattt aaaatcccat tcatcaaatc aattgaagca acaaaatttc tgttttcttc
    25741 gggaattttt ttattgtgca aactttgtgt aacataataa taacaaccca cagcatcttt
    25801 tgttaaagca gaagtcaaag ccatctgcgc agcaaatttt gcaggattgt cctttgccgc
    25861 tttatacatc ttatttaaaa gcttactgtc taataattta ttaaatacag gcattatgtt
    25921 agggctcata atgttttacc tccctcttga ggttttttcg cggggcataa ttgagggaaa
    25981 aatttatcca ttattctggg gtaagaccaa ttcaaaaatc cgcaggcaaa aggcagggtt
    26041 ataagagaaa gcactattcc ctgagcctta gtgtatgcct taaatgcggt tgttgcatca
    26101 tctactgaat tttttgcaag ttgttttgca aagtttttca accttgaaat gtcattttct
    26161 gtaagtattt ttttaacatt gaattctttt aatgtttccg taaaggtttt tcttgaagac
    26221 gaagaagtgc tgtcactcaa catttcgtgc atagtgagca taaatttatc taaagttgaa
    26281 tctaaaacag ggctttgatg aaaatctttt tctattccca aaatattaaa taaagccttc
    26341 atcgaaatgt tgtttttacc tttagcttct tctgttataa gattttctgc tatttgttca
    26401 ataatatctt tttgatttga atcaaagcct tttgtactta taattcgatt tagagctttt
    26461 cttaaagatt tttcgtcaat tgttgcatca tcaaatccta tttttcttaa aaaatctatt
    26521 gcagtggttt tttcaagttt tgatttcacc atataaggat taagcatttt ttcgtcaatg
    26581 cagtcttttt caaaatccat atttttaatc aaaaaatcac gtaaggtttg atttttgatg
    26641 ctgttttgag agctttccga aagaagtttt tgagcaactt cttccaaagt tgttttatga
    26701 actgcctgtt caaaatgctg aaaagtaggg aatttttctc taaaagcttt aacatcttta
    26761 aaatcttgag tgcctgcttt ttcaaaatat tttttatcga ggtctttcaa tttaccgaaa
    26821 ttctttacaa tgctgttata tcttgccttc agaaagtctg attgaggttt tgccgacata
    26881 tcaaattttt ccaacgctcc ttctgagcat gctttgctta aaccgtctgc aataggttta
    26941 ttaattccaa aacccaaagc cgttgctata actgcagaaa taggctgtct cattgcagaa
    27001 tattttttct ttttggggtc ttgttttgat aaagagttat aagctataaa aataggggcg
    27061 acaaaagccg ttccaaagcc tatcaaaaga aggttttgaa tctctcctgc cgtttttgaa
    27121 aaataatcca aagttcttcc gaaaaagcct aaatactttg tttttaaagc ctgttcaact
    27181 atcaactctt gtgctgcggt caaaacatct tccttgcctt taaaagacac ttgcggcttt
    27241 tttaaaggct tttctttaaa atttgtgctc tgaacttgtt gtactaaatt tacttttgac
    27301 attacggcca cactttcaat gcatgaaaga aaactgaaga ttaaaaattg acttttttag
    27361 tttatttttt taagattttt catattttgt tacaattaaa aaaaataaaa attaatgcta
    27421 aaattaatct atgaggagat attttttaat aattttatta ctatttctat cttgtgcaaa
    27481 aagctttgcg tttgaaattg tctatccgaa aaaaacgccc tgcaccatag atgctccttc
    27541 gacttttttt ataggttcga ccaatccgca ggatattttg aaaataaacg atgccgaagt
    27601 tccgcttcat aaaagcggct cttttgcttt tgtagtgccg ctcaaagaag gtataaatga
    27661 atttaaaatt acttcttacc cctcacccct gccctcttcc tacggtaccc ctcaccccaa
    27721 ccctctcccg caaggggaga gggagaacaa ttataataaa atcattagtg caaggggaga
    27781 gggagaaatt aatcctgtat caattgattt tattattgaa cgaccaaaac cgcaaacaac
    27841 aaaaaaaatt gaaccaattt tgctggaata cccgccgatg aataattttt ttataaaaga
    27901 tgacaacacc cctttgaggc ttaccccttt tgacagcgga atcaaccgca tggcacacct
    27961 tcaaaaagaa acccctcttg tgataaacgg cgaaaaaggc gggttttgga gagtgttttt
    28021 aaattcaaag acaattggct ggattttaaa atcagatgtc gagcaaaaaa ccgattcaac
    28081 aatttctgca ctgcccgtaa gcattaaaaa acttgaaaca aaagaaacaa aagatttttg
    28141 catatgggaa tttgaacttg aagggaaaac tccttttgta ctgaaagaag aagataatcg
    28201 ccttaattta aaacttttta acgtcaaatc cgaagacaaa aatctttgta tagaagattc
    28261 ttctttgaat tttgatgtgc ctgttaaaaa actcttcggc tatgacgcat attttgaagg
    28321 gaataaattt attttaaaag taagaaaggc gcccgaaaaa aactgcgata tgccgcttaa
    28381 gggcttaact ataaccgttg atgcgggaca cggcggggct gaatacggcg caatcggacc
    28441 aaaaggcgat aaggaaaaag acattaacct tgaaattgct aaaaatctca aagcagaact
    28501 tgagactctg ggtgcaaaag ttataatgac aagaaaagac gacatagccg tttcgctcaa
    28561 agacagggta aaaaccgcaa gggaaaatga ttctgtaatt cttataagca tacacgcaaa
    28621 cgcgcttcct gacggagcgg atcctataaa atgccgcgga acaagcgttt attactatca
    28681 caatcaggca aagcctcttg cagatagcat tttaaattca attatcacaa aatcgggaac
    28741 tcaaaacgac aggatgcggc agggaagcct tgcgcttgtg cgcccgacat caagtgtgag
    28801 cgtgcttgtg gaggttgcct acattataaa ccccgacgac tgtgaacttc tgcttgacaa
    28861 agagttccaa aaaaactgcg caaaggcaat tacagaggga gttgtaaaat atttatccga
    28921 ttaacttttt tgcagatgat aaatctttta ccattacatt tgttttatcg ggatatcggc
    28981 acagagggct gggatgcaaa atcggcattg ccttataagt tttgtttgaa acagatattt
    29041 caaaaacttt tccgtgaact tctgtaattt ttttcccctc cagaaattct tccatcgcag
    29101 ttgagccgca taataatatt aatttagggt taattgttaa aatctgctct gttaaaaaat
    29161 gcctgcatgt gcttttttct ttttttgtcg gaaccctgtt gtttggaggt ctgcatttta
    29221 ccatattaat tatgtataaa tctttttccc ttgaaattcc ggctttttca agaaattcgt
    29281 tcaaaagttt tcccgccctg cccacaaaag gaatcccctg caaatcctca ttttccccag
    29341 gagcttctcc aattaaaaca attttggcac tttcagggtt tccgtctgaa aaaaccacgt
    29401 tatttcttgt tttatgaagc tcacatgcct tacactcact gcacttgcgc ttcaattccg
    29461 caagcaattt ctctttcatt acttttttcc ttaaaaattc ctatcgaata ttttctacag
    29521 tattttttca actcttttat aacaacattt gaaagcacaa aaaccgcaaa caaattaggc
    29581 acggcaagaa acagggtaaa agcgtcagac acattaatta tgctcttaaa attcatcgca
    29641 gagcctaaaa ttatgaaaac acagtatata acctgaaaaa cttttgtatt tttgccactt
    29701 tcaccaaaaa gaaaatgcca cgccttaagc ccgtaataag agccgcaaag cagggttgag
    29761 gttacaaaac agcttaccat aacagtcaaa agcagaggga agaaagaaaa tgcgcttcca
    29821 aaagcacggg aggtcaattc gattcctgca agccccgatg aatttaaata ctcgcccgaa
    29881 acgattataa caagggcggt tgcagaaccc acaattacgg tatcaacaaa gggctgaagc
    29941 attgcaataa aggcctgcgc cacaggtttg cttgtcttaa ccgcagaaaa agcaatcgat
    30001 gctgttccca agcccgcttc gtttgaaaac atcgcccgcc tgaagcccca caacatagaa
    30061 acaaacattc cgcctgcaac cgcctgaggc ttaaaagctt cttttaaaat caaaacaaaa
    30121 gaataaggta tgcgctcaaa attcatcaaa caaacaacca aagcggtcaa aaggtagagc
    30181 gaacacataa cgggtgtgac tttttctgtg aattttccta tggactttat tccgcctatt
    30241 atggtaaacc atattatcag agccacaatt aagcccaatg cccaaccgtt attgtgaaaa
    30301 atgcttgcat tccctcctgt aacttctgtg atttgagttg tcatggcatt aatctgaaac
    30361 aaattccacc cgccaatcat tgcaaaaacg cagcatatcg cataaatttt tgcaagatgc
    30421 ggtgcaattt ttgaaaggta ttttcccttg agccctttta aaatataata cataggacca
    30481 cctgccaccg ttccgtcggg gttaactttt ctgagtttta ttcccaaaag tacctctgaa
    30541 aacttcaatg ccatagaaaa aagccccgcc aaaatcatcc aaaacaaaac accgggacct
    30601 acaagagaga ttgcggctgc tgttcctgca atgtttccca tgccggcaga tgccgaaatt
    30661 gtagcactta aagcttgaaa agaagaaact tctccgattt ttttcttttt tgaaactgcg
    30721 gggtgtttat acttatttga aataaggttt agggcatgtt tgaagcccca aaaccctata
    30781 aaacgggttc ttattgtaaa ataaagtgcc gcaaacatta aaagcgcaac caaaagctgc
    30841 acctgtaccc cgcctattgt tatatgacaa aaaatcaacg ctgaaatttt atcggctaca
    30901 ggggacaaaa aattatctat ggcggcatct aaattaacat tcaaaatcat ttcaacaaaa
    30961 ttatactata aaaaccctta gcaaacagct gagtaaaatt ttcaaaatga atattgtagt
    31021 cttgaaagca aaagtctatt aattttctta aactaccttt atcccttata ttttggaatt
    31081 atgtattcta ttttttattt gtcattttct aaaagtgagc tagaatttta ttgtaaaaat
    31141 attttaaaaa atgtttataa atattctaaa tgtggaagta taacaaaata gggaattatg
    31201 aaaaaaacag ggcttacatt tactgaaata cttctgtctt ttgggattat agggattctt
    31261 gctgcgataa ttcttccggt tttacagagt acaagacccg agcccaatgt tgttatgttc
    31321 aaaaaagctc ataaaatttt tgttaaaaat gtatttgatt tgattaataa tgaaacttat
    31381 tatccttcga ataaaacggg acttacagcg ggaaatttgc ttaaaccaag agggtttaac
    31441 tataccgatc ttgaagggct tccggacaaa acggacaaat tctgttatct gttttcacaa
    31501 aaaataaata atttgaatga agtaagatgt aaatctcccg gcaacggtta ttttacaaca
    31561 acagacggaa tttattggga aatgaaactt ccgtttgatt ttcctatgtc ggttaacggt
    31621 tacgaacagg taatggttga tgtaaacgga cctcaaaatc ctccgaactg cgaagaatca
    31681 tactgcaaaa aagggattgt acctgataaa taccctctgt atgtaagata tgacggaaaa
    31741 atgaaaacaa gtcttttggg cggggttata acaataaatc ctacaattca gactaaaaag
    31801 gttttaacaa ttaccccaca gcctgatact ccggaagttg agccgacccc gccggatacc
    31861 tgtactccta aggggaatat aaaagtgggg aatttatgtg tatctccgac aaatataatt
    31921 tatgttccgg tcagttatcc gccatacaat gacaaagaca attactggaa gggcgcagat
    31981 aacgcttgcc gcgcttacgg tatgagattg ccgacaaaag ccgagcttca ggacatgtat
    32041 aaacaaagaa aagaaatagg gaatatgaat ccgggttggt attggtcagg cgaagaaatc
    32101 tcaagcgatg aaaatcctac agggcctcct tctacttggg attatgccgg ggcttatgat
    32161 tttacaaata aaggcggaaa tgtcgtaaac ggaaaacatg accaaggcgg aagagcccgc
    32221 tgtgtcctgc aatggaatgg ggcggggtat tattgatttt taaagagtat tttttgttcc
    32281 atttcttttc tttttttctt gcgattgaaa aaaagaaaag aaaccgaacc gtagtcactg
    32341 ttcgtgacgc cattttgcac tgaaacaagt ttcaggcaaa agaaggcaag aaaagaaaaa
    32401 aacacgcgaa caaaatgcca aagcgtcagg ggcaagcccc cgaaccccca aattgctcgc
    32461 tccgatatgg tcggaggggg taaatatgtt tggcttaatg gctgaaaagc tgaacggctg
    32521 aaaggttata aaaataagtc atactgagca ttctgccgac aaaacgatta aatatcgttt
    32581 tggagaggaa gaagaatctt gtccgaccac gtcgttgcga gcgaagcgaa gcaatccaaa
    32641 aaacgaactt tgcgcttgaa tttttgatga aattaattta ttatatgtaa taaatttatg
    32701 tatagtaaaa tcgaaaaaat aaacaaactt gatatcgcat caagcttaac aaaccaagct
    32761 ataaggatgt tttttcataa agataatcca atcagtaccc atgttatagt ttctgctgca
    32821 aacgaaatat taataaattt aattaaaaaa aatggtttaa aaacaaattt aaaaagcaaa
    32881 tacggtagat tctctaacga atcgaaagtt taaaatttaa aaaatggaga cgaggactct
    32941 gccgaattaa atatgactaa atgtcatatt taatgagagg aaagaacccg aaaatctttg
    33001 attttcgcgt gagactcccc tcgaattgca agagtcgtaa atctttagtg aaaaaatgga
    33061 gacgagggga gttgaacccc tgtccaaaat ggattagagc aaatctctac gagtgtagat
    33121 tttgtctttc tcactaaagc ccaagtaaaa tcagacttgt tagctttagc aaagcacttt
    33181 taagcgatac ccgctttgag aaaagtcttg atgcgcttaa ttctcatccg cgcactgcaa
    33241 cttgcataat tgtccctacc cgccggcaag ctgggctgat agagaggctg actgcctaaa
    33301 aaattaagca gctagagcag cagctctaga aaaatcagct ttaactacgt tgtttgtagc
    33361 atttgttttt ttgctcgttg ataactcgga acagcgccga gactcgcaac ttactgtaat
    33421 cgaccatcct gtcaaagcca agcgtcccca tgaatatact ttgacccctc accctgtttt
    33481 ctaatgctcg caggcttctg ctcaccctga aaacagacct ctcccgcaag gggcgaggtg
    33541 aaaattcgga gtactttttt tcaatgttct gtattataag tataacccac ttttttaatt
    33601 tttcaacccg aaaaagcttt tatcaaatta taatcttatc accttttacc ctttcattta
    33661 ccccaaagtt taagaatagt aaagagaatg gtttttcccc taaaatctgt tataatattg
    33721 ctacactggg ggagaaatga aaacaaacaa aaaaattaat ataaaacccg ctgataaaat
    33781 attaaatctg gaaaaatata tttttgccga acttgacgag tggaaacaag aagcgcgtgc
    33841 aaaaggtgcc gacctgattg atttgggcat aggaaatccc gacgggccaa ctccaaaacc
    33901 tatagtaaaa gccgcaataa aatcgattaa aaaccctgca tgccacggtt atccttgttt
    33961 tagaggcaaa caagaatttc aaaatgcgat taaagaatgg ataaaattcc gctacgcaat
    34021 aaaattagac tcagattttg gaatacaaac attaagcggg gcaaaagaag ggcttgctca
    34081 cacggcactt gcttttacaa accccggaga tataaacatt gttcccgatc cttactaccc
    34141 tgttttgtca cgcggaactt ggatttcaag cggggatgtt tatcacgttc cgctaaaagc
    34201 agaaaacaac tttttgcccg acctggattc aattcccaaa gatattgcaa aacgcgcaaa
    34261 aatgtttttt ataaattatc ccaacaaccc gacaagtgct gttgctacaa aagaattctt
    34321 gaaaaaactc gttgattttt gcacaaaaaa caacattttg cttgtctatg accttgctta
    34381 cggtgaaatc tgctatgacg gttacagacc gccgagcatt ttcagcattg aaggcgcaaa
    34441 agatattgca atagagtttc acacattttc aaaaaccttt aacatggcag gttggagagt
    34501 cggatttgta atcggcaaaa aagaatttat cgatgcaatc catgccatga aaacaaatac
    34561 cgattacgga acttccacca ttgttcagga tgctgcaatc gaagcactta aaatgcctta
    34621 tgaaaacgtg gaaaaaatag ttaaaaaata tcaaaaacgc agagatttta tggtatctgc
    34681 gatgaaaaaa cttggttggg aagttaacaa aacccctgct acaatgtatt tgtggctgaa
    34741 agttccaaaa ggaatcggct caaaagaatt ttgcagaaaa acaatggaca aaacaggagt
    34801 ggtctttacc ccgggtgtgg cattcggaaa acactccaat ggatttttta gggtttctct
    34861 tgtgcaaagc ctttcaaaat taaaagaggc cgtaaaaaga cttgaaaaag caaacgtaag
    34921 atatgaggaa taagttgaaa atttacagta aaatttttat aattttatca gtttgtgccc
    34981 ttggtacaac acttcttccc gccaatgcaa aattcaatgc gggaatttca aaaaaccatt
    35041 tttacgacac ccaaaagaca attatgaaag aaatttcgcc caacattgtg ccaaatactg
    35101 acaattactg ggaaaaaaac acttttttca caaacaaata tcttccgctt tttatgctgt
    35161 caaataaaga tctgataagc tatttgcccc caaaaaagca caaaataata aacagaatga
    35221 taattccttc tgtatataac gtttctttta aaacagctcc cgttacaaat gtttctctgg
    35281 gcggaggcaa aaaaaacgaa gtcgtttcaa atcaagagtt tgatgttaaa tggatgatgc
    35341 tttatgaaca agcaaaaaac aaagaagcag acagtgacaa aaaaatcgaa acggcagtcc
    35401 ttttaaaaga taccaaaaat atttcaaatt ataatttagc aatagatttg cttgacgatg
    35461 ttacaaggca agagccttat aacgcttatg cctataacct taaaggcgat attttctttg
    35521 tcaaaaacga caaggaaaat gccatgaaaa attatgttga agcattgaaa ctgaacccct
    35581 tgagcaaaca aagctgcctt ggaattgcaa aaattcttga acccacaaac aaagaacttg
    35641 cccaaaaata ttttgaaaaa gccaagtaaa aattaccaat ctaagtcgtc gccgtaagca
    35701 gtactatcat tatagtattg gcgagggcca tagtattgat atgtaattgt ttgaatatct
    35761 tttggtaagg ttttaacaaa attaccaaag gcatcacgga caattctttg agagtattca
    35821 ccggcaatgt aggattggtt ttcattgtta cgaccgcgaa taaaagcaaa atctattttt
    35881 gctttttcag gtaaagtttt aacaaaatca agaactttta aagccacttt tggctgattg
    35941 ttttttacag ctaaaataaa aatattttca ttattatcat tttgcactat agggatacaa
    36001 ctttggatat ttttgggtaa tttttcaaag tgtttgatga tttcaagagc tttttcttgt
    36061 ttattttcaa ttattgcatt ttgtaatatt ctatattgct ctttagcttt ttctttggat
    36121 aaatctgagc aattaagcat tatgggaaaa tgaagcggct cattgttttg gcgtttattt
    36181 tctgcgggtt ttttgcgcat aaatgtttga taagaaataa tattgacttt cataaaaaca
    36241 tctccttgtc ttttaataac attttaaaac gcaaaagaaa aatttttgtc accttgggaa
    36301 taaaatatac acttcttaat aaattaatat tcttgcagta aatcttcttc gtcgtctttt
    36361 aacaaagaca aatcatcact gtatgtagca tcaaaatctt caggaagcat ctcgggctcg
    36421 ggccaaattg tttcttcatc aaagcggcag gcattcaaat taaaacaccc tgcaaaatcg
    36481 gaatttgaca aatcgctttc ctgaaaaacc ccgcctgcca tatccgcctc ggggaaattg
    36541 cagtaattaa ttgcagcata gctgtaatct gctcctgtta aaaccgcctc tgaaaaatca
    36601 cattccaaaa tagtcgccct tgtaaaatca acagaggtta aatctgccct gctgaaattt
    36661 attgaggtta aatgagcgtc tgcaaaagaa ctggaattta aatcaacgtc tgaaaaatca
    36721 acatcactta agatgcagta agaaaagttc atttcactta agtcaacttc atcctgccct
    36781 tttttccatt cgttaaatct ttgcggcgaa gccttaatta tgttgtacaa atcctctttt
    36841 gaataatcgc tcatctttta cctttctcct taattttact gtttaatcaa attcatctgc
    36901 atagccaaaa gcactgcctg cgtgcggttt gtgactttca gctttttaaa tatactgttg
    36961 aggtgggttt ttaccgtaac ctcttttaca aaaagcttat ttgcaatagc ctgattgtta
    37021 gaaccttttg cgacaagatt taaaacctct atttccctga gcgtaagcgg ctcaacaggt
    37081 tcatcagacc caaagggaag ttcgtggact tttgtgtttt ccttattcca gacagagcgg
    37141 cgcaaaaccg cctctattct tgcaagaaga tttggcagaa caaacggttt tacaatataa
    37201 tcgtcagctc ctattttaag ccctgatacc attttttgct cttcatttac tgccgttagc
    37261 ataataacag gcagatgttt tgttctttca ttatttctta tagattttaa agtatcccaa
    37321 ccgttgagat tcggcatcat aacgtcaagc aaaacaaggt catacttttt ttctgctttt
    37381 gcaagaattt tgagagcctg aacaccgtca actgcaactt taacctcgta tccgtacatc
    37441 ggaagagcat cttgtaaata tttaggattg tcatccacta ttaaaattct tgccaactgc
    37501 gacataattc accctacact atcttgtatt ttagggcttt cagagctgcc tgaagcctgt
    37561 catccacttc cagtttttgc aaaatacttg ctacatgggc ttttgtggta tttatactta
    37621 tgaaaagctc ttttgcaatt tgattattac taagaccctg cgttattaat tttaaaattt
    37681 gagcttctct tgttgttaga ttataatcgt ttttgctttc attttcaaca aaattttcat
    37741 tcacagccgc cctcaaaaca acatgggcaa tactggggtc aaaccatgaa gccccttcca
    37801 atacagattg gacaacctca agcagccgtt gagggttaat ttcttttgag caataagcgt
    37861 tagcccctgc tttaagactg tttaaaacct ctttttcgtc gttgtgggaa gttaaaacaa
    37921 caacttttac gtctttattt ttgttcctga ttttttttgt tgcttctatt ccgtttatgt
    37981 tagggagccc caaatccata attattaaat caatattgtt attattaatt atctcaaaag
    38041 ctttttcagc acaatcagct tcaaaaaccg ccttgacaaa gttttgtccg tcaaaagtag
    38101 ttttcagccc aaatcttgta agttcgtgat cttctactat aagtatattg ttttttttca
    38161 ttagatgtcc attattttat ttggcttacc ggagtaatag gatttggttc gacttctgtg
    38221 tgcattaatt cattttttgc cggctcaaaa tcggatttca gttttaagca ttttttaaaa
    38281 ttatattctg cctgagcacc gtttcccgtg tttttttcga ccaatccttg ataataataa
    38341 tacctaaaat cattttcgtc aatacaaaaa gcgttggata aatatttttt tgcatcgtca
    38401 taattttgtc ttgaaatgct tcccttggca agttcaatcc atgcctgttt aaacatagga
    38461 tttattgaaa gtgactgttt aaaatattct tcgctgttgt tttcgtttaa aagtcccaaa
    38521 taataataag cttgccaact tttaggattt tcttttatcg ttttttccca aatttccttg
    38581 gctttttggt catttgaaag cttttgccat gcgatacctt cttttatttg cggcaaataa
    38641 tcttttttat tttttgaagc cgcctgttta taatatttta aagcctgctg aaaattaccc
    38701 tgattacagt ttaaatcacc caaaataaga agggtttgag gattattttt atcaatttta
    38761 taagcttttt gaacattttc tgaagctttt tcaaaatcag aagtttcaaa ataaattttt
    38821 gccatcaagg aataaatggc ttgagtatca actccctttt tacctgaaag tgctgtttga
    38881 agagttttta tagcttttga agagtcattt tcaaggtaat aatagtagcc caaatgataa
    38941 tttctttggg aagaagtttt tcctgttttg taaagagtat attgttcaag tgcatttatt
    39001 tttctttcgt attcgtaaga gagcagattt tgttttttaa gtgaagctat aattcttaaa
    39061 gccgcttctt gcctaccggt ttcggcaagg atttcgactt taagtttttg ataatttaaa
    39121 gtcgaaggac ttttacttat tgcagagtca atatatttgt ttgccttagc aaatgcacct
    39181 gttttataat atcccagagc cataatataa tttgcataat cagaagacaa aagaagcgac
    39241 gaatttttag aaagttcttt tacggtttct gtgctttggt tgttataaat tatgtttgaa
    39301 taggattccg caagcaaaat ttcttgttct tccgttaatt tttttgacgg ataataaaac
    39361 ctttttatag cttttgagga attttttgaa aaatcactgc ttatttttga aattgccaaa
    39421 tccgataaat caaaaaatcc caatcccgcc attttatcag ccattgttag gtaaaaaaag
    39481 tcgtttttat cagccttttg aatgatgttt ctaaaatctt gcttggatgc tgctatattg
    39541 cactggacaa atctgtccgt tgcgtttgaa aaatcataag tatcggataa atcgattgtt
    39601 cttacaaggc ccttttgtgt ctgcaaattt attgtttttt caaaagccga aggtttagga
    39661 taaaaagagt taataggtga aaaatcaaca gctaagactt tttgcgaaaa ataaaccgcc
    39721 ccaaataaaa ttattaaaga tattaactta aatttagaat aggccattaa ttttggtcat
    39781 ccttgcttga ttttgccgta taataaaaat taaacaaatc aatgaaaatt ttttcgtctt
    39841 cgctcaactc ttcaggttgt ttttgtgttg aaaggttata taaatccatc attgaatact
    39901 tttcaagaat ttttttgtgt tcttcctcga aggttaaatg attttcagtc ataaacactt
    39961 caacaatctt gtttgtcgga atcgaaagaa aacattgaat aatttgagaa tcaaaatgtg
    40021 tccccgaatc tttcaaaagt atttcaatta cgttcataat aggcatttta tcacgataat
    40081 gcctttttga tgttatagca tcaaaaacgt ccgaaactgc caaaaccctt ccgcccaaag
    40141 gaatatcttc accgcttaga tgtctgtaat aaccgtttcc gttaaatttt tcgtgatgcg
    40201 agcaggcgat ttctgttacc agtttaaatt catccgacat gtgaattttt tccaaaatat
    40261 tatgagttat ttctgcgtgt tcctggatgt gtttatattc ttcgtctgtt agttttcctt
    40321 ctttttgaag cacagaatct cttattccga tttttcctat atcatgaagc gttgctgctt
    40381 tttcaattat ttcgacattt ttttcatcca tattcattgc ctttgcaata agtgaagaat
    40441 aaagccttac ccttgtggaa tgacctgccg taatcttatc tcgagcgtca atagatgcag
    40501 ccaaagtatc cataaaacta tcaaaaactt gtttttgttc ttcgagcatt tcgccttgtt
    40561 ttttgaaaag ctgggcattt tcaagtgcaa tgcccgcact tgaacctatc gcgacaagca
    40621 aatcttcgtc ttcttcttta aactcgccgc catttttgtt taaaacctga aaagccccga
    40681 ttatttcctg attaaggttt ttaatcggca tacaaagaat ggttttagta acataacctg
    40741 ttttcaaatc aatttctttg ttaaatctgt cgtcagaata agcatctttt atattaattg
    40801 tttcgcctgt ttgcacaaca tgtcctgcaa gccctttgtc cgcgggaaat cttatttctt
    40861 cgctttccat gccaagtgct acttttgacc aaagctcgtt attttctctg tcatataaaa
    40921 atacgctgca tctgtctgcc tgaattgccg actttgtttc ttgggctatt acctctaaaa
    40981 gagtgttaat atcggtttcg gcagatacag tctgacctat ccttaaaagt gccacaagcg
    41041 gatcatgttt ttccggtgtg gaaaaatcgc aactactatt taattgcttc attcttgaat
    41101 ttatatgttc aataagtccg tattcatctt gtctttttgc tattttaagt gcattttcat
    41161 agtgaattat cgcagtacaa gaattttttt caataaaatc caaattcccc aacataaatt
    41221 cgtttataag ttttgcagta gtattttttg tacattctgc aagatattta ccgtcattca
    41281 gataagtcaa tgccgacaga tatttgcttt tatcacccaa atactcaata acacccaata
    41341 aacttaaaca tattgaaata ttttcacttg atttttcctg aataaacaat ttttgagcac
    41401 ttaaagcagc actgaatgct tctttataac ttttattaag aaattcgtct aaggcttttc
    41461 ttataagaaa ttccccttgt tttatatgtt cattttttat gttttccaca accatgctac
    41521 aaaacctctt aacaaactct tattttgatt atacaatatt ttttaggata actcaaaaaa
    41581 aataataaaa tagtatattt acagacaggg aagaacaaaa aatgcagaca ataacaatac
    41641 ttgtacccat ttttaacgaa gttaatacgt taagaattgt tttacaaaag cttgaagaag
    41701 ttgatttttt tggtttaaaa aaagaaatta ttctcgtgga cgattgttct acagacggaa
    41761 caagagatat tttaaaacaa ttacaggatt ataaaatttt ttatcacgaa aaaaacatgg
    41821 gtaaaggcgc agctctaaga acaggttttg aaaacgcaac aggagatatt atcataattc
    41881 aagatgcgga tttagaatat gacccgattg attatcagcc tttgataaaa cttatattag
    41941 aagaaaaagc tgatgttgca tacggatcaa ggctttcggg cgcaagacct gcgcgttctt
    42001 ttatgtttac tcacctttta ggaaacaaat tccttacact tcttacaaat attttataca
    42061 acacgacctt aaccgatatg gaaacctgct acaaagcatt taggacagat tttataaaag
    42121 ggattgaaat aaaatctgac aggtttgatt ttgaacccga aattacagca aaagtcctca
    42181 aaaaaggtgc aagactttat gaattgccta tttcttatta cggcagagaa tattccgagg
    42241 gcaaaaagat tacctggaaa gacggttttc atgctatttg ggcactgatt aaattcagat
    42301 ttgttgatta aatttttcct ccccaatagg gcgatttttg tatctttcct gtaacatttt
    42361 gttaaaaaat aacaattttt ttc
//
LOCUS       DAOQAU010000009        40700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_840_length_40700_cov_7.014713, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000009 DAOQAU010000000
VERSION     DAOQAU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 40700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40700
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_40700_cov_7.014713"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1314)
                     /locus_tag="PKI94_02740"
                     /pseudo
     CDS             complement(259..1314)
                     /locus_tag="PKI94_02740"
                     /inference="COORDINATES: protein motif:HMM:NF021096.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
     gene            complement(1280..2233)
                     /gene="dcm"
                     /locus_tag="PKI94_02745"
     CDS             complement(1280..2233)
                     /gene="dcm"
                     /locus_tag="PKI94_02745"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNW25688.1"
                     /translation="MKFIDLFAGIGGIRLGFESVGFECVFSSEWDEQACQTYFANFGE
                     MPAGDITKINPDEIPNHDILLAGFPCQPFSIIGAKAGFEHETQGTLFFNIEKILKVKQ
                     PRAFMLENVKNLIAHDDGKTFSIIKKHLKALDYKVYYKVLNALDYGVPQKRERIIIVG
                     FKDDVNFEFPKPMPKGNRIDISKILEPETFIDKSLYVKDYIRERRNGLMKIKPDYDYI
                     SHENIGGNVTPHRYSCALRAGASSNYLLINNKRKPSARELLKIQGFPDNFKIVVPYTQ
                     IKKQTGNSVAVPVIKAVARQIKKALYEKEINDRQSKSKICA"
     gene            complement(2287..3300)
                     /gene="aroF"
                     /locus_tag="PKI94_02750"
     CDS             complement(2287..3300)
                     /gene="aroF"
                     /locus_tag="PKI94_02750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNW25689.1"
                     /translation="MLIIMTHGATEENISSVKTFIEKKGFSAHISVGEIHTVIGAVGG
                     KIIDSRDIELLDGVKEVIRISSRYKLASRVFQQEDTVVEVNGVKFGGEHLGIIAGPCT
                     VESYEQTDKTAKALSKSGVKILRGGAFKPRTSPYDFQGLGEEGLKIIREVADKYKMAV
                     ASEIMEISQIPLFEKYVDIFQIGARNMQNFNLLRELGQVKKPVILKRGLSATIEEWLM
                     SAEYILSGGNRQVILCERGIRTFEMVTRNTLDLSAIPVVKKISHLPIIIDPSHGTGLR
                     DKVIPMSRAAVAAGCDGLIIEVHHDPDNAICDGAQSLHPQEYDTLYQSIKKIAQIVDK
                     KVL"
     gene            3409..4704
                     /locus_tag="PKI94_02755"
     CDS             3409..4704
                     /locus_tag="PKI94_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW25690.1"
                     /translation="MKNKTDVIVVGGGPAGISAAVTIARAGKSVILIERGNFSGSKNM
                     FGGAIYVQPTKEIFPNFEQSAPIERKTVIHKYLMLTKDDAVGISYFDKKSEVNSYSVI
                     RAKFDRWMAQEAQKEGVVLVTETVVKDLLIEENKVVGVITELEEYYSDIVILADGVNS
                     LLAKKLGLRKEIKPKDVALGVKEVIKLSKEKIEERFNLEDGEGSIYEIFGEPMLGMLG
                     LGYVYTNKESVSIGLGVGLDELAKRNLKPYELLDQLKSHPRLEPLLKDGELVEYSAHL
                     IPEGGFNKIPKLFSDGVMIAGDGAMLVNNLHWEGTNLAMISGKLAGETAIEALTKGDF
                     SANMLALYQKKLENSFILKDLKSYKNLMNIIHDRAESFLGYYPEKIASFFKMFTTVDS
                     IPKRDKYRSFIKSIFTDRSISELFKDFVNIVKLMLGVLK"
     gene            4704..5006
                     /locus_tag="PKI94_02760"
     CDS             4704..5006
                     /locus_tag="PKI94_02760"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNW25691.1"
                     /translation="MDENQEKSKPTIDDKLFKVKYVPDEESHLKADNKICLLCTSKVC
                     TYICPAHVYEWDEENKKLLIGHENCLECGACRIVCPSKNIDWEYPKGIKGVTFKNG"
     gene            5041..5406
                     /locus_tag="PKI94_02765"
     CDS             5041..5406
                     /locus_tag="PKI94_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25692.1"
                     /translation="MKEEYSPNFRRWYDKDPVLSSAMKTLENSDDETQIKIALNLIKI
                     IIEHNIEESEYEAVEDIISAVEAGVEEERSSRWYDIDSTLKTAMSMLQNCPEKTQNII
                     AKEMAKMVVAKIKEEEIIE"
     gene            5418..6164
                     /locus_tag="PKI94_02770"
     CDS             5418..6164
                     /locus_tag="PKI94_02770"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNW25693.1"
                     /translation="MSGHSKWATIKRKKAKVDSARAVVFQKYSRELMVSARLGGADSA
                     ANFRLRTAIDRAKSAGLPADNIKRAIEKGAGLGSAENFEELTYEGYGAGGVAIFIEAL
                     TDNRNRTAGDIRSYFTKYNGNLGENGCVGWMFKPVGIVSFSKLQDFEKLFDAAADLGA
                     DDVMEDDENYKVITSVENFQSVVEGLEKSGFKNISAELTRLPQNSIEITDEKIASQIL
                     KLLERIEEHDDVQNVYSNFDIADDILQKIE"
     gene            complement(6165..8012)
                     /locus_tag="PKI94_02775"
     CDS             complement(6165..8012)
                     /locus_tag="PKI94_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNW25694.1"
                     /translation="MRILNYNGQITFGSGNKFAINRNKKQEGESELAILFSNDAHGKI
                     GKLPNLKTARDEFVSSFKDKDAASFVLTTGDNFIGSSSKIYTIWSEFLNNFFKPEAQT
                     IGNHEFDHSFEHCAKIINNSNIPTLAGNLFALENKMFDDMKKNGKLINSTVIEKSGQK
                     YGFIGVTATDIFEYKSRESVDGLDVFDFEKTREIIKNEIEKLQKQGINKIILLSHLGY
                     IPDLLIAGAFENIENEKEKNILSKTISLKGIKTFEGLRGIDIIIGGHSHEEIQGSNSI
                     EKIRQLNKDYSSSDLEILTSLKGTYVNLVTPENAEPVLIAQGGAENNYVGFIDVIFNE
                     KGAIKKDKIINKLLSVSDFKENKKVSAYISKIIGKKQDILTLKTPFVDPQRKSENAIQ
                     NMLADSIIKKANHELPKADIALIHGAAARGGLSGKVSNWDIEHDILPFSDKVYMIKLS
                     EKDLVDTVNNTAVRSLVLIGKPVLMHPSKNLRYTISNTPKILENGKKLYVRDITITDK
                     NGKERKIDLINPSVKKEFNVLVEEYFLQGEPCLGGKEGFEALALEGFAKYIAGLAQKP
                     EGILKEYNWSSKDVFIEYLNKKYKGKEIEFLPDGRITIIKDEPVETKTA"
     gene            complement(8113..9192)
                     /locus_tag="PKI94_02780"
     CDS             complement(8113..9192)
                     /locus_tag="PKI94_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25695.1"
                     /translation="MQINLSSQYKTQSKTFSQQSFSGYFACPIKEIHIHPVAEVDGVA
                     QELQQKCSNYFDIILQTDNEIVNLNKAKPNNYINTRLKPWSQDYKVFLESGTVGSLFL
                     NAEKALLELSEFLGLNCKQISSDFAGGNFFIGKKNDGETFMLLGEKSLFNKDGRTISK
                     DFEIKKGNILKIHQSDFHIDMTVRPLIFPDVLVADEDMLLNLLMKEPEVKRNKKLKNK
                     ILKMYNHRKKYQNIERYKETKQIIKEIEEHGLNTIKVPGILGENNLNFMNAIVHQNPD
                     GSLIYITGGSTLFKKEGINLNKIFEKALKNAVPQIKDVIFIECKDKIGETILEGTLKE
                     IHGGLHCMCCERPDFERWNKLINEK"
     gene            complement(9509..10177)
                     /locus_tag="PKI94_02785"
     CDS             complement(9509..10177)
                     /locus_tag="PKI94_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25696.1"
                     /translation="MKKILVLLVIISLTLPAFAQGTKKTRLDKCFEISRQAEARKDSE
                     LFKDYAMCVYRTNKKGIVPKDEIKVLDNQALGLIVKYYTTRYSQTQNKKDFEKAQKYA
                     FAALKNRTNDPETIYNTILLFALALDEENMLLTYDYWQKISPEDSARINSEFGQNIEK
                     VRETKSLLKHNRNYYMKKRIGTILYSVGYDMPSYAMEAGDYHKKEVPPCIYSIENLQY
                     KFEY"
     gene            complement(10257..10670)
                     /locus_tag="PKI94_02790"
     CDS             complement(10257..10670)
                     /locus_tag="PKI94_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNW25697.1"
                     /translation="MKFKTEYLWFNTEKHREYKNIKDQVQDIVTQSGVQEGMVLVSAM
                     HITAGIYVNDAESGLISDIDKWLEEIAPFNINYNHHRTGETNGDSHLKSLLIGHEVIV
                     PITKGQLDFGPWQQIYYAEFDGQRKKRVIVKVMGE"
     gene            complement(11132..11662)
                     /locus_tag="PKI94_02795"
     CDS             complement(11132..11662)
                     /locus_tag="PKI94_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25698.1"
                     /translation="MEEQEINVLENKPQRNWKNVIKISLITLAILLTVGFLLRLYIMT
                     LPINGDDIYCEYKGKFNKIVCVQTGDIVPDWLDLDDSISRGLFGGNVDYSMRLIFAKD
                     TNMYTSLKMVDKVFVTDLRRKFIYEKAEQTLSDYIKFKKFVLGSKMSNWGSVVLYCSN
                     SNLTEDVCKQMKSRHK"
     gene            complement(11834..11908)
                     /locus_tag="PKI94_02800"
     tRNA            complement(11834..11908)
                     /locus_tag="PKI94_02800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11874..11876),aa:Val,seq:gac)
     gene            complement(12185..12255)
                     /locus_tag="PKI94_02805"
     tRNA            complement(12185..12255)
                     /locus_tag="PKI94_02805"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12221..12223),aa:Cys,seq:gca)
     gene            12850..14802
                     /locus_tag="PKI94_02810"
     CDS             12850..14802
                     /locus_tag="PKI94_02810"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNW25699.1"
                     /translation="MNDKLKLSENAVKVLEKRYLKRDKEGKCVETPADMFNRVASSIA
                     NAELKYGKSQAEADSLSKKFYEAITNLYFMPNSPTLMNAGRELGQLSACFVLPVEDSL
                     EGIFETIKNTALIHKSGGGTGFSFSRLRPKNDVVRSTMGVSSGPVSFMEVFNAATEAV
                     KQGGTRRGANMGILRVDHPDIIEFIHCKNDNNKLNNFNISVAITDEFMNALKNNQDYN
                     LYHPKSKQAVGKLSAKQVFDLIVDGAWRNGEPGIVFIDKMNDDNPTPLVGDIESTNPC
                     GEVPLLAYEACNLGSINLGLMLKETSNGLDVDWNKLEEITKLSIHFLDNVITINNYPL
                     PIIGEMVQNNRKIGLGVMGWADMLMKLGLSYSSKEGTDLAYKVMEFIDYHSKIESLEL
                     AKSRGAFNNFKGSVYERGNFLSNKYKGKSAGMISDEMWADLDAKIKQFGIRNATTTCI
                     APTGTISMIAAASGGVEPLFGLVFLRNVMDGTEMLEINPVFEKYAKENGFYSENLMKE
                     ISKDGSVAHVNNVPQKAKQIFVTAHDVTPEWHVLMQAAFQLHTDNAVSKTINFEEHAT
                     REDIEKAYILAFENNLKGITVYRNNSRTFQPMNLDAKKTPQIEIKPIDEPTGELKTVV
                     CPECGNSIEMAEGCFICLNCGYSGCS"
     gene            14820..15659
                     /locus_tag="PKI94_02815"
     CDS             14820..15659
                     /locus_tag="PKI94_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW25700.1"
                     /translation="MKGEEKITIITASYNYARYIEEAINSVFAQTYDNWELIIVDDGS
                     TDNSVEIIKKYCQKDDRIKLFQHENGQNRGLKETILLGLENATGDWVAFLESDDVFKP
                     DNLLKKIEVIKNNPDTNFIFNSVEFIGKENMPQYRIKDFEKTQEKLSKMIFPKNLFFD
                     FMQKNLIFTFSTVAVKTEVLKNVDFKSPCDVFLDWWLWIHLAYRNEFYYINEELTQWR
                     IHPESYIKKSKKPILFQLDIYGDIYKKNKSDLKVLAFIFASVPELILTKAKGFVLKTL
                     QGK"
     gene            complement(15709..16242)
                     /gene="cobO"
                     /locus_tag="PKI94_02820"
     CDS             complement(15709..16242)
                     /gene="cobO"
                     /locus_tag="PKI94_02820"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNW25701.1"
                     /translation="MLEHGYIQIYTGDGKGKTTASLGLALRALGHGWNVLIIQFTKGD
                     SATSYGEIVSASKFLPNLDVMQFGMDRVVYSHNICMEDFKEAKKGWEFAKEAIQGGKY
                     QLVILDEINICIAMNMLKVSEVKEVLLNKPENLEIVLTGRYAHPELKALAHLVTEMQP
                     VKHYFDMGVMARQGIEY"
     gene            complement(16747..17199)
                     /locus_tag="PKI94_02825"
     CDS             complement(16747..17199)
                     /locus_tag="PKI94_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25702.1"
                     /translation="MKKILALLIIGALVILTLSFDSAFAAKKGGKGKGLTQEDLDTMS
                     ASVDTLTKKIYANGLFSPTDNENLIDIKIKLDNQMLVSPDPSLAPLYYKVGVLYKSRQ
                     MKDEAVECFQTILENFADTALAPKATAQLKSMGVEVKAPEKKEGDAAE"
     gene            17306..18283
                     /locus_tag="PKI94_02830"
     CDS             17306..18283
                     /locus_tag="PKI94_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNW25703.1"
                     /translation="MNKSTKEKLYQMFILGLEGETLSGNPNLKAALQKGLGGVIFFTQ
                     NIKTEKQFKNLINEIKKEALIFPFLSIDQEGGRVERTENIHDGKKYLSPKYAAQKGEE
                     FLKNQTEQIACELKDFGINLNFAPCVDVNTNPDNPIIGERAFSDKTDEVIKFGKIVTQ
                     EYLKNGIIPCLKHYPGHGDTNQDSHLTLPEINLPFEEMEKIHIKPFKEISAPMIMAAH
                     LHCACFDKEKIPTSLGKNAIGYLRKNLGFEGVIITDDMVMQGALGFESTKACEMAIKA
                     GVNILLYRNSFDETIQIIENLAQKALLDKELKENIEISFEKIMKLKIKN"
     gene            complement(18498..19316)
                     /gene="proC"
                     /locus_tag="PKI94_02835"
     CDS             complement(18498..19316)
                     /gene="proC"
                     /locus_tag="PKI94_02835"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNW25704.1"
                     /translation="MNKIFNQAELKVGFIGCGKMANAMIKGVLSSGFLGSDNIIASEI
                     SQGLASVKKSELGINILLDNCEVARICDVIFLAIKPHFIKEVLQEIKEELQGKLVVSI
                     AAGVKTKSIEDQIGEVAVIRVMPNAPAVIKEGMSGIVKGNFADEDDVNFVKEFMSNIG
                     KCIVVEEAQIDVLTAISGSGPAFFYKIIHEMALAGEKLGLDYEKSKFLAIQTAIGSAK
                     LMQSSNLNAQDMINMVATKGGCTEVGVEYMNEINTKELFFELIKRTAKKSKELG"
     gene            19411..19563
                     /gene="rpmH"
                     /locus_tag="PKI94_02840"
     CDS             19411..19563
                     /gene="rpmH"
                     /locus_tag="PKI94_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNW25705.1"
                     /translation="MRRTLEGTKIKRQKVSGFRARMATDGGREVLNRRRARGRHKLAV
                     EGSKRA"
     gene            19759..20106
                     /gene="rnpA"
                     /locus_tag="PKI94_02845"
     CDS             19759..20106
                     /gene="rnpA"
                     /locus_tag="PKI94_02845"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNW25706.1"
                     /translation="MRLKKDSAFSATYRIRHSYAFSGIVLWVGKLKTDFQTPTKAGFV
                     VSKKTHKRAVKRNRLKRLMRESYRLLLKEGKIEKSQKFMSLIFVGQEKALGQSFLQIN
                     ETVLKLLNGIREK"
     gene            20103..20624
                     /locus_tag="PKI94_02850"
     CDS             20103..20624
                     /locus_tag="PKI94_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25707.1"
                     /translation="MIEGLIAIQTQTMDEFFPYPPYPMYPSGLIDESKSIYRYAHPSS
                     DCPVLVIKDPIYDEDRNYIPPGHYSLVLSDDRTFLLLVQGDKIFATIPVFKLEESKEE
                     VEKIYDKKYQKQQAKEKKKQDKINAKLARTGAPPQEKPVYMEATIEYDNIERYYLIKY
                     ERGFVRAWGAIKK"
     gene            complement(20803..22080)
                     /gene="obgE"
                     /locus_tag="PKI94_02855"
     CDS             complement(20803..22080)
                     /gene="obgE"
                     /locus_tag="PKI94_02855"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNW25708.1"
                     /translation="MQFLDKATAKIISGHGGNGMVAWRREKYVDKGGPAGGDGGNGGD
                     VYFIADENMSTLMDFKYKSVFKAEDGVNGRSKSQHGKCGKDLYINVPVGTMVKDLKSG
                     KIIGDLTKNSDKILVAKGGRGGRGNARFATAQKRAPQFCEPGEPSIERDLELELKLIA
                     DVGLLGMPNAGKSTFISKISSAKPKIADYPFTTLVPHLGVVKKPSGDGFVVADIPGLI
                     EGASEGIGLGHEFLRHVERCRFLVHIIDLTMEKPVENYNKINLELKKHSQKLSELYQI
                     IALNKIDVISIKDRKNFFKKFKEISDDVFLISAVTGENIEELKLFIAQKVDEIEKPAF
                     EVDVEEDLDAYNNDDSLFEVVKVDKNAFAVVGGKIKRLAQVSDARNTEQVLRLQNIMK
                     NMGVMEALKKAGIKDGDTLIIGHLELVYFDDEV"
     gene            22158..23204
                     /gene="tsaD"
                     /locus_tag="PKI94_02860"
     CDS             22158..23204
                     /gene="tsaD"
                     /locus_tag="PKI94_02860"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNW25709.1"
                     /translation="MNNEKKTLFSNKTVKIGENSGYDNYIMAIETSCDETACAVVKNG
                     REVLSNVVASQIKTHAKYGGVIPEIAAREHLEAINIVIEEAFEQAKIKSEEITAFAGT
                     VGPGLVGSLLVGLNAAKTLALVWDKPFIGINHLNAHVCANYIDTDLKPPFIALLVSGG
                     HTQIIEVKSHSEQKIIGETIDDAVGEAYDKVARLIGLPYPGGPLLDKMAKEGNPNAYK
                     LPEAKVEGYDFSFSGLKTAVLRLVQSLKTASDELPVNDICASFQETVSETLLKKVKRA
                     LEETGYKQVVLAGGVAANSEIRRKFFELENQGYKVFAPQMKYCTDNAAMIASCAYFST
                     NTFDDVNVEVFSRV"
     gene            23280..23543
                     /locus_tag="PKI94_02865"
     CDS             23280..23543
                     /locus_tag="PKI94_02865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNW25710.1"
                     /translation="MEKYAEIFKALAHPIRLKIVCGLCGKDECNVSKMVDGLKIAQPT
                     VSQHLNILKNAGIITGFKKGTQICYRVTNDEVKKIIKSLEIKK"
     gene            23540..25909
                     /locus_tag="PKI94_02870"
     CDS             23540..25909
                     /locus_tag="PKI94_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW25711.1"
                     /translation="MKILIVGGGAAGASCAARLRRLREDAQITILEKTGEISIANCGL
                     PYFCSDVISDRDKMLVSNPTVFKELLNIDVRLHSEVTLINRLNKSVKINNDYEMSYDK
                     LVLALGANSIKPPIEGINAQNIFTVRMLEDADKIKEYVSKNEVKNTVVIGGGFIGVEM
                     AENLAHLGIKTSLVELADQILAPLDAEIAAFAQNQMRDNGVELILSDGVKTFEQKEIE
                     LNSGKKIPYDLAILAIGVRPEISLARECGLDIGQTGGILVNEFMQTSDENIYAAGDSV
                     EIIDFVTNSKALIPLAGPANRQGRIIADNIAGLNSTYKNSQGTAVVKVFDLTAASVGN
                     NEKQLLKKEIEYKKVYIWGNSHAGYYPGSFPLMIKLLFSPEGKIFGAQAVGFDMVEKR
                     IDVISSVMRFGGKVQDLIDSELCYAPPYSSAKDLVNIVGMAADNILKKLSKPAFYEDL
                     QDAFIVDVRPEISFKVKTIEGAVNIPVTQLRWRMGEIPKDKKVVLFCDKGFNSYVAAR
                     ILIQNGFENVLNLSGGFLLYKEIAKDKKGIVALNSNPNTTTLGSVLTLDACGLQCPGP
                     ILKLSEAIKELKEGEIIEVLTTDSGFALDIEGWCETTGNTLLNVSRENKMIKASIKKG
                     EGKVMIQQTKDGQTIVVFSNDLDKVLASFIIANGAVASGKKVSMFFTFWGLNVLRKSG
                     NVKIKKNFIEKMFSFMMPKGAQKLILSQMNMGGLGTLMMKWVMKSKNVFSLPELIKTA
                     QQSGVKFIACTMSMDVMGIKEEELIDGVELGGVAKYIAETNSANSNLFI"
     gene            complement(25902..27488)
                     /locus_tag="PKI94_02875"
     CDS             complement(25902..27488)
                     /locus_tag="PKI94_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25712.1"
                     /translation="MKIDNNLNTKNNIQFKGPFDAPITQALMNIDTNPMLNAALVDTV
                     AMVIPRTYVDSKKRNKYAGAETFFREATGTITVCLSSGVIASGIAYLYNKFFDKKTNI
                     KPNLWITNKTFDILKHSWDKHKNSKSYVAEVLQNISGQDGRKNVSWKDIQWGKVDWYE
                     HPTWDNIRWHDKAFANIKNESKSEEQIIKFLSELINNKTASRHDVKNALKILNYRISN
                     ALGVSNSLTVKVEDKILNTSLNNLIRDIYDAGKNIFTNETLDLTKAGKKLKSMNTTKT
                     LGALAIASSLGLINQYVNRQITKKRTGSDAFVGHENFGSKVQKQTKKNNKGLTSLKIL
                     ASAGIFTLAMGVMKIKNPADFIKRLEFSGPVTSGNAIKTVYTATLIGRFLAAKDKTEL
                     QESATRDYLGFLNWLVLGGFVAKGVGQMLFDKKMNTLFNINKKGNGILHWLNDITPKS
                     HMEIAAKGKDFAKENIWKINFMQASGLIYSTLALGFALPLLNIFIYKMKNGKNQAENI
                     KQPTNLLQKPEKPKAFANFR"
     gene            complement(27734..28441)
                     /locus_tag="PKI94_02880"
     CDS             complement(27734..28441)
                     /locus_tag="PKI94_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25713.1"
                     /translation="MKKYWFILILILFLISKNDAFATQTVFNVPSADVTEKGHVFLQE
                     EAQFQPWNPGANFVNTFYADYGVGHNTEVGAILFNVGAPATQNISMGVGFKTAMPIAG
                     LKEKFPEREFKFTIGSQVLIGMEGNGVGNWTYAHLSGRVPKVNTRLTAGMSYGGRQVF
                     GVNHTCFIAAVEQPVTKKFSLIGDWYSGEEHWAGFLIVGGSYKLPKNTAVYAGYQIPN
                     SPQNGTSGFVVQLSKIF"
     gene            28667..28870
                     /locus_tag="PKI94_02885"
     CDS             28667..28870
                     /locus_tag="PKI94_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HNW25714.1"
                     /translation="MFNFLKYTSYVLVLVGAINWGLIGLFGFDLVATLFGEMSYVSRV
                     VYSLVGISAIISAFTVYYSCKEN"
     gene            complement(28932..29252)
                     /locus_tag="PKI94_02890"
     CDS             complement(28932..29252)
                     /locus_tag="PKI94_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25715.1"
                     /translation="MKIATLFKELCCSRCKSDFDEDSVEIMREEDEMLVFRLVCRQCG
                     KSFGVAFLGIGDFELKNYSQGDKVLKMKGEPEPINEDEVLDAHKFIKEFDKNWRKFIS
                     DLDE"
     gene            29618..30559
                     /gene="rsmH"
                     /locus_tag="PKI94_02895"
     CDS             29618..30559
                     /gene="rsmH"
                     /locus_tag="PKI94_02895"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNW25716.1"
                     /translation="MTDFKHYTVMKKEAVDALGCKAGLIYVDATLGGGGHSKLILEKI
                     QPKGRLIAFDVDKDAIDAAKEKLKGYDNLTIVQDSYTNITEVLQDLKIDKITGGIIFD
                     LGASYHQLTQAQRGFSFSQNAPLDMRFNKDADFSAYNVVNSYSEKDLIRIFSEYGQER
                     FSKRIAKKIVEQRKVKPIETTGELADLIIECTPRIKSSIHPATRVFQAIRIEVNQELT
                     NVKNTLEKVFHLLGDGAIISVISFHSLEDRIVKNLFREYSQKCRCPKNQMICTCKPPM
                     LEVITKKPIVASEKEVKENPPSRSAKLRIAGRVLARE"
     gene            30565..31029
                     /locus_tag="PKI94_02900"
     CDS             30565..31029
                     /locus_tag="PKI94_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25717.1"
                     /translation="MIQRQAQIIDNYGSSQGYYQGYQSNPQYYNPIIEGYFPKENTRK
                     DMAIRKINLTLTTLLGVFIVLTSISYYFSSANEMVLNDLNRQTVVLNDENSDLQNKLD
                     RLKSFNNVDMTMQKNNMLQKAQQVIEVRAVSTATPSDKKIDAQKPFTWAIGY"
     gene            complement(31168..31662)
                     /locus_tag="PKI94_02905"
     CDS             complement(31168..31662)
                     /locus_tag="PKI94_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNW25718.1"
                     /translation="MTVTDKIKSIVGFLTQGFEQRDDVFEDMADELGMEYPIEDNLAL
                     EPMYSSNYRSEKQSDLKVVAHPNYKSYEVMVIEPRSFGEAGQIVQNLKEKKTIVLNLH
                     LLDKEQSQRTVDFVCGAAQALNGKPQKISEMVFIFTPNNVTLSVDAAANVNKFRDSLW
                     NQPQ"
     gene            complement(31683..32345)
                     /locus_tag="PKI94_02910"
     CDS             complement(31683..32345)
                     /locus_tag="PKI94_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNW25719.1"
                     /translation="MGSIEENVLKIQEEIAPYKPNIIAVTKYFDESKIEEAYQAGLRD
                     FGESRVIYAIEKIQKLSQDTRQNSKFHLIGHLQSNKARKAVKFFDLIHSVDSLELAKT
                     ISQEAIKEGKIQKILLQLNNAQEEQKFGFSKGELFAIFEQIKELKGIEIKGLMNMAPF
                     GATSPELKELFEDVVKTRNELEKKYHCELKEISMGMSGDYKEACEAGATMIRIGRKLF
                     ST"
     gene            complement(32589..33986)
                     /locus_tag="PKI94_02915"
     CDS             complement(32589..33986)
                     /locus_tag="PKI94_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNW25720.1"
                     /translation="MSQALYTTMGGISAAQTQINVVSNNIANINTIGFKESNITFQDI
                     FSTTLTGGNSPTVTTGGKNPIQIGLGVQVGTISKNMDSGTWTATGKATDMMIQGNGFF
                     TVRSSSGQFFLTKAGNFTFDANGDMVNAQGYKVVGASQLYAASSSNATVKVPQQIVSS
                     VTANNAMASKALSELNDCQITTGDIFIEVNTSATSQTVPVSLEATDNTIQKICDKIQK
                     AIDDKMLWTPTTRGLTIKCDTSTGGKIKFELNQTIAPPNRVNNINFGPGTANPSNFFE
                     QIELSSLNSDTHEYTSKILDYQVDVSPVTSTTSAIAVSNYSIAGDGSIEATYSNGDKI
                     TIELNESDNTFRFKYTTSTGVIIRNDNVHVNPNVAQPPNFQIQLANVINPEGLVSEGG
                     NLWSMGANSGTPIYTVGGTMGVGAIKSGGLEASNVDISKQFSNMIMAQRAIQANSRVF
                     GVTSDIMETLVMLGR"
     assembly_gap    34168..34267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34268..34699)
                     /locus_tag="PKI94_02920"
     CDS             complement(<34268..34699)
                     /locus_tag="PKI94_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNW25721.1"
                     /translation="MAASVQNQITDMQNATAQYQASLPKTTGTSQFDADTFLMLMLKE
                     LQYQDPLSPMDNKEFLAQQAQFTQVSATQELKENMATNNYIMQTLALVGKEVTITDPD
                     NPRQTITGTVTEAGFENGGSCITINGKEYPLSLVQSVREPGT"
     gene            complement(34727..36265)
                     /locus_tag="PKI94_02925"
     CDS             complement(34727..36265)
                     /locus_tag="PKI94_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNW25722.1"
                     /translation="MATAITDLLTQIKTKSESSKKTYTDKSEAFENVFDKVSKNYTKE
                     ENKFNSEDKKVDYQKKADKYEQKEEDKSENTALKDNSQKNSEKENKIEENTNLPKDKP
                     SQDSKQDKIQESKTQQKESYDEGKTAEAENKVQADPEQKNQAQSTEQSKTSITVPVAS
                     EAADILLKNLQIATAQNQVQPQIPTQTQIQPVTDKTKVQTQPQTQQVLLNINIENISQ
                     DNTTPEITNQIPKNAQTDIEQINADIKAKTPVIQANIDVKTKSDENPEQKQSIKDVLQ
                     KTILTQEMLDATNARVVSVEQNASSDNLLNKQSAQENGIKSLMDSNQQNNQNLNITDA
                     KASFSKTIESIQSPKEISKNDILAQIHTKLTEAKDEGSTKVTIVLKPENLGKINLELI
                     NGKDGLIARMTAENVQVKELLEKNLEGLKNSLGAQGVQINEVNVKVENIEKQSNEMLN
                     FEYQQQTMQDEQEQKDSQKADNKEQNVQNNSLDSSEDSDISEENAEETVISEKHTGRV
                     DYKV"
     gene            complement(36277..36729)
                     /gene="fliJ"
                     /locus_tag="PKI94_02930"
     CDS             complement(36277..36729)
                     /gene="fliJ"
                     /locus_tag="PKI94_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNW25723.1"
                     /translation="MKKYKFRLQTVLGMRQKVLEEKQLEMAKVVSLLNMQLQVLEDLL
                     NKKELTKKNLEKIYENNAELDILEITNHKNFFSKMINNIKIQEEVIKNIQIVLNAKQQ
                     EVTEALKEVKILEKLKEKQEKKFYQHYEYLQGKEIDDIASTRYQKMTA"
     gene            37009..39102
                     /locus_tag="PKI94_02935"
     CDS             37009..39102
                     /locus_tag="PKI94_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25724.1"
                     /translation="MFLIAFAQIVLTFEVLSLFSAIKPFQALGLNILFLIFSTSFWFK
                     RSKPLWSLDLSDFKNKIFNSFKLDKSLIWLFVGFCIFIFVSLVLCFVSPISSADASHY
                     HLARSLFWVLQGSLNHFETADIRNLCFPINSEILYSWILLFAKKDVFLGFFSFVGYVL
                     SVVSIYSTLGLMGFCTRKKLWAIFILSSFSSVIVQVSGTETDVIVAGLITSSIFLYWH
                     ALKYDQKMPIFMSSLAYALAIGTKTPAVMAIPGVGLLFLFLSIKFRAKEFYKPLLLFL
                     GFGLLNFIIFASYNYVLNFIHFQNPVSSKGLLMTTKNYYGVKAIPASFIKYIFLFFDF
                     TGFRWSDYVGQYFTDFKNILLNFLHLSYIKDGLYSMDTGPNRTLLEPVMGAGILGFLV
                     FLPCWFWSLIRPLFKPKSKKGILLFIFGLILVINISLMSYLIVFMIYSIRFLATFIII
                     SAPVLVFSYFKRKNVFKFVIVVFALFYLILVSTHLWARPFIKMSKLFYKHPSISYLRE
                     AFKCRGENDFNAYSNATCVLRDVIKNKISEENKILALFSSSQDIFTIKRLEFSGYKID
                     FARAENIKNIDLKKYNIIIIDFNDIKATNITEYEKRKDETKIENNKIITKEGNIFPCI
                     YNFNETLPKSLLNDKEKIYPYVSQCIISKEFIKENKLYILYKVARPLSENRFEGFIML
                     KNQNNPLILHNNFKK"
     gene            complement(39106..40494)
                     /locus_tag="PKI94_02940"
     CDS             complement(39106..40494)
                     /locus_tag="PKI94_02940"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNW25725.1"
                     /translation="MYGIILAGGSGSRLWPLSRELYPKQLLNLTSNESLLQATFERLQ
                     NFMPSKNIISITNTKHVSSVKYQLESLAKNPVVLSEPLSKNTAPAIALATKFIQQQEK
                     DPVIIVVPSDHLIKNTKKFTQTVKKGEKLAKEGYIVTFGIEPNYPETGYGYINTLRHP
                     LLDGFKVKEFVEKPDAKTAQKYLDAKTYFWNSGIFMFKASTMLKEIEKLAPEIYSVMK
                     KFDFSSSDKIEYNLFDKMPSISIDYAIMEKSDKIALVKLESDWNDLGSWQSIYDVGQK
                     DKNNNVIIGNVIDENSKNSLMYSSSKLVATIGLEDTVIVETEDAILACKKDKTQDVKK
                     IFDKLKKINDTTHLVHKTVYRPWGFYTVLAQGIGFLTKKIHVNIDQKLSVQSHNHRSE
                     HWVVLSGKAKVILDGKEHILTPGHSIDIPLKAVHSLQNPFKEDLEIVEVQLGETLIEE
                     DIVRYEDIYGRV"
BASE COUNT        12345 a         7041 c         7122 g        14092 t
ORIGIN      
        1 taccttctta aggtcgggca cggaaaacac taaaaattgt aaatttttgt attttccgtg
       61 ccctcttcaa ggtaatatta atattttttt gtttttttaa atgttttatt taaaaatttt
      121 ctgttttaaa gcaaaaaaat gaaaaaattg aacaaaaatt ttcaaaaagg gcaaaaatcc
      181 gaaggatttt tgcccttttt gaactaaatc tcaaaaataa attttgatac gtttatttac
      241 aatttaaatc ataaaatact attgccaaaa atcgtcttgt attaattcat attttcttga
      301 tgaaagtata ttatttttag tattaacact aggaaattcg gcagatattt cttcgcataa
      361 acattcaatt aatttatcgc caatgatatt tgaatatttt cctcttaggg ctttttcata
      421 ccaattaatt aaatcatctt cagttattat actttggatt tttgatttcc agccaatttg
      481 agttaataat gaaacaatta aacctttttc tgctgtttta caaacgataa ctattttgtc
      541 actagagaca gaatcaacaa tattttcagc tagaacttca tctaatgcaa gatgttttat
      601 ctgaatagca ggcccccaat ttgcatacat atctaagcct ctatctgcgg catttgttac
      661 acctacccta taaacttttg cagattgcat gtaaactggt ttagaaaatt ctatacacat
      721 aacatttttt gtaaagtctt caaattcttt aagtacatat aatttatctt catttattga
      781 aaccttaatg attaattcaa gctcatcaac caatattgaa aacaaagagt aaacaataat
      841 ttcatatatt ttatctaaac ttcttcttaa tccggcttca tgccaaaaag aatctattaa
      901 tgtttttata ttaaatgttt cttttgtatt ttcataacag tattttaatg ctgaagataa
      961 ttggctatgt ctattatcaa acattctata gatataagct tctacagcac cattcgtacg
     1021 attattttca tcaccaagaa ctttaagaac atttggaggt acagcattat catcaaataa
     1081 attatcttgg aatttagcag aactagtaca aattctgcca agtaaatata aagatacttc
     1141 atctcttcat tttttacttt ttgttcgata gctttctaaa tttaataaat ccatttgagg
     1201 gtttaatctt ttttgatata agatttctgc tatctgtatg ggtttgtata aatgcaccct
     1261 tgattttttg attagtttat caagcgcaga ttttgctttt gctctgtcta tcattaatct
     1321 ccttttcata tagtgctttt ttgatttgtc tagctaccgc ttttataaca ggaacagcaa
     1381 cactattacc tgtttgtttt tttatttgag tgtaaggtac aactatttta aaattatcag
     1441 gaaatccttg tatttttaat aattcccttg cggagggctt ccttttatta ttaataagta
     1501 aataatttga gctagctccc gctcttaatg cacaactata ccgatgaggt gttacattcc
     1561 ctccaatatt ttcgtggctt atataatcat agtctggctt tattttcata agaccgtttc
     1621 ttcgttctct aatataatct tttacataga gactcttatc aataaatgtt tccggttcta
     1681 atattttaga gatatcaatc ctatttcctt taggcatagg ttttggaaat tcaaaattta
     1741 cgtcatcttt aaagccaaca atgataattc tttctcgttt ttgaggcact ccataatcaa
     1801 gtgcatttaa aactttataa tatactttat aatcaagtgc ttttagatgt ttttttatga
     1861 tgctaaaagt ctttccgtca tcatgtgcta ttaagttttt tacattctct aacataaagg
     1921 ctcttggctg ttttactttt agtatttttt cgatattaaa aaataaagtg ccttgtgttt
     1981 catgctcaaa cccagcttta gcacctatta tactaaaggg ttgacaagga aaacctgcaa
     2041 gtaaaatgtc atggttagga atttcatcag gatttatttt tgttatatca ccagcaggca
     2101 tttcgccaaa attcgcgaaa taagtttggc atgcctgttc atcccactcg cttgaaaaaa
     2161 cacactcaaa tcctacgctt tcaaagccca gccttatccc tcctattcct gcaaataaat
     2221 cgataaattt catatttaaa ttataatcta aattatatgt ccgtacaaaa aataaataaa
     2281 tattatttac aaaacttttt tatcaacaat ttgtgcaatt tttttaattg actgataaag
     2341 agtatcgtat tcttgcgggt gcaaagactg tgcgccgtca caaattgcat tgtcgggatc
     2401 atggtgaacc tcaataatca atccgtcgca acctgctgca acagccgctc ttgacatagg
     2461 gataactttg tctctgaggc ctgttccgtg actgggatca attattatag gaagatggct
     2521 tatcttttta acaacaggaa ttgcagatag gtcaagtgtg tttcttgtaa ccatttcaaa
     2581 agttcttatt ccgcgctcgc aaagaattac ctgtctgttt ccgccggaca aaatgtattc
     2641 tgcagacatt aaccattctt ctattgttgc ggataatcct ctttttaaga ttacaggttt
     2701 tttaacctgt cccaactctc ttaaaagatt aaaattttgc atgtttcttg ctccaatttg
     2761 aaaaatatcg acgtattttt caaataaagg aatttgagaa atttccataa tctcagatgc
     2821 tacagccatt ttgtatttgt cggcaacttc tcttattatt tttaaacctt cttcgccgag
     2881 tccctgaaag tcataaggcg aagttctggg cttaaatgcc ccgcctctta aaattttaac
     2941 cccgcttttt gaaagagctt tagccgtttt atccgtttgt tcatagcttt caacggtaca
     3001 aggtcctgca attattccaa gatgttcacc tccgaattta accccgttta cttcaacaac
     3061 cgtatcttct tgttgaaaaa ctctgcttgc aagcttgtag cgcgaagata ttcttataac
     3121 ttctttgaca ccatctaaaa gttctatgtc tctagaatca ataattttgc cgcctacagc
     3181 accgattacg gtatgaattt cgccgactga aatgtgggcg gaaaaccctt ttttttcaat
     3241 aaaagttttt acgcttgata tgttttcttc agttgctcca tgcgtcatta ttataagcat
     3301 tgcatatcct cgattcctaa atttaccccg attgtacaat aaatttaaac aatttacaaa
     3361 cgagtttttt cattatataa ttatttcata cttcgaggag aaccttaaat gaagaataaa
     3421 acagatgtaa tagttgtagg cggaggtccg gcaggtattt ctgcggcagt gacaatcgcc
     3481 cgagcaggga aaagcgttat tttaatagag cgcggaaatt tttccggaag caaaaacatg
     3541 tttggcggtg ctatttatgt tcagccgaca aaagaaattt tcccgaattt tgaacaaagt
     3601 gctcctattg agcgaaaaac cgtaatacac aaatatttga tgctcaccaa agacgatgct
     3661 gtcgggattt catattttga caaaaaaagc gaagtaaaca gttactctgt tattcgcgcc
     3721 aaatttgacc gctggatggc tcaggaagcc caaaaagaag gcgtggttct ggttacagaa
     3781 accgttgtta aagaccttct tattgaagaa aataaagtcg tcggagttat cacagagttg
     3841 gaagaatatt actctgatat agtaattctt gcagacggag tcaattcgct tcttgcaaaa
     3901 aaactcgggc tcagaaaaga aattaagcca aaagacgtgg cacttggcgt taaagaagtt
     3961 ataaaactaa gcaaagaaaa aatagaagaa agatttaatc ttgaagacgg cgaaggctca
     4021 atatatgaga ttttcggtga gccaatgctt ggaatgttag gtttgggcta cgtttatacg
     4081 aacaaagaat ctgtttctat aggtttagga gttggcttgg atgaacttgc aaaaagaaac
     4141 cttaagcctt atgaactttt agaccagtta aaaagccacc ctcgccttga gcctttattg
     4201 aaagacggcg aattggtgga atactccgcg cacttaattc ctgaaggcgg ttttaataaa
     4261 atcccgaaac ttttttctga cggggttatg atagcaggtg acggtgctat gttggttaat
     4321 aatcttcact gggaaggtac aaatcttgcc atgattagcg gaaaacttgc gggcgaaaca
     4381 gcaatcgaag cacttacaaa aggcgatttt agcgcaaata tgcttgcttt gtatcagaaa
     4441 aagcttgaaa attctttcat cttaaaagat ttgaaaagtt ataaaaattt gatgaacata
     4501 attcacgaca gggcagaatc attccttggc tattaccctg aaaaaatagc atcatttttc
     4561 aaaatgttta cgacagttga cagtatccct aaacgtgata aataccgttc tttcatcaaa
     4621 agtattttca cagacagaag tatatccgaa ttgtttaagg attttgtgaa tattgtaaaa
     4681 cttatgttag gagtcttaaa ataatggacg aaaatcaaga aaaatcaaaa ccaacaatag
     4741 atgataaact ttttaaggta aagtatgtac ccgatgaaga atcacaccta aaggcagaca
     4801 acaaaatctg tctgctgtgt acttcaaaag tctgcacata tatttgtcct gcgcacgttt
     4861 atgagtggga tgaagaaaac aaaaaactct taatcggaca cgaaaattgt cttgaatgcg
     4921 gcgcgtgcag aattgtatgt ccgagcaaaa atatagattg ggaatatcct aaaggcatca
     4981 aaggggttac ttttaaaaac ggttaattaa agatttagtg tttataaaaa ggagaaagag
     5041 atgaaagaag aatacagccc aaatttcaga cgttggtatg ataaggatcc tgttttgtct
     5101 tcggctatga aaactttgga aaattcagac gatgaaacgc aaatcaaaat tgctttgaat
     5161 ttaattaaaa taattattga gcataacatt gaagagtccg aatacgaagc agttgaagat
     5221 attatctccg ccgttgaagc aggggtggaa gaagaaagaa gttcccgttg gtatgacata
     5281 gattcaactc taaaaactgc tatgagcatg cttcaaaatt gccctgaaaa aacccaaaac
     5341 attattgcaa aagaaatggc aaaaatggtc gttgctaaaa ttaaagaaga agaaattata
     5401 gaataaggta taaattaatg tctgggcact ctaaatgggc aacaataaag cgtaaaaagg
     5461 caaaagtaga ttctgccaga gcggttgtct ttcaaaaata ttcaagggaa ttaatggtat
     5521 cggcaagact tggcggagct gattctgcgg caaattttcg tctgagaacg gcaatcgaca
     5581 gggcaaaatc agcggggctt cctgcagata atattaaacg ggcaatagaa aaaggcgcgg
     5641 ggcttggaag cgcagaaaat tttgaagaat tgacttatga aggctacgga gcaggcggag
     5701 tagctatatt tattgaggct ttaacagata atcgcaacag aacggcaggc gatataagaa
     5761 gttatttcac aaaatataac ggaaatttgg gcgaaaacgg ttgtgtaggt tggatgttta
     5821 agcctgttgg cattgtatca ttttcaaagt tgcaagattt tgaaaaatta tttgatgcag
     5881 cagcggattt gggtgcagat gatgtaatgg aagatgacga aaattataaa gtcataactt
     5941 ctgttgaaaa ttttcaaagt gtagttgaag ggcttgaaaa atcaggattt aaaaatataa
     6001 gcgcagaatt aacaagattg cctcaaaaca gtattgaaat tacggatgaa aaaattgcaa
     6061 gccagatttt aaaactttta gagcgaattg aagagcatga tgatgtgcaa aatgtttatt
     6121 caaactttga tatagcagat gatattcttc aaaaaattga gtaattaagc tgtttttgtt
     6181 tcaacaggct catctttaat tatggttatt ctgccatcgg gaagaaattc aatttctttg
     6241 cctttatatt ttttgttaag atattcaata aaaacgtctt tgcttgacca gttatattct
     6301 tttaaaatac cttcaggttt ttgggcaaga cctgcgatgt attttgcaaa gccttcaagt
     6361 gccaaagctt caaagccttc tttgccgcca aggcagggtt ctccttgaag aaaatattct
     6421 tcaacaagga cattaaattc tttttttacg gaaggattaa ttaaatcaat ttttctttct
     6481 tttccgtttt tgtcagtgat tgtaatatct cttacgtaaa gttttttccc gttttctaag
     6541 attttggggg tatttgatat ggtatatcga aggtttttgc tagggtgcat taaaacaggt
     6601 ttacctataa gaaccaaaga cctgacggca gtattattta ctgtatcaac caaatctttt
     6661 tctgaaagtt taatcatata aactttatca gaaaatggta aaatatcatg ctcaatatcc
     6721 caatttgaga ctttgccgct aagccctcct ctcgccgctg ctccgtgtat tagtgcaata
     6781 tcagcttttg gaagttcatg gtttgctttt ttaattattg aatctgcaag catattttga
     6841 atagcatttt cgctttttct ttgaggatcg acaaaagggg ttttaagtgt taaaatatct
     6901 tgtttttttc ctattatttt tgaaatatat gcggagactt ttttgttttc tttaaaatca
     6961 ctcacgctta aaagcttatt tattatttta tcttttttta tagcaccctt ttcgttaaaa
     7021 atgacatcta tgaatccgac ataattattt tccgcaccgc cttgagctat caaaacaggc
     7081 tctgcgtttt cgggagtcac aagatttaca taagtgcctt taagcgaggt taatatttcc
     7141 aaatcacttg aggaataatc tttatttaat tgtcttattt tttctatgct gttgcttcct
     7201 tgaatttctt cgtgtgaatg tccaccgatt ataatgtcaa tacctcttaa tccttcaaaa
     7261 gtttttatcc ctttaagaga aattgttttt gaaagaatat ttttttcttt ttcgttttct
     7321 atattttcaa atgctccggc aataagcaaa tcagggatat acccgagatg ggaaagtaaa
     7381 ataattttat ttatgccttg tttttgaagt ttttcaattt cgttttttat aatttctctg
     7441 gttttttcaa aatcaaaaac atctaagccg tcaacagatt ctctggattt gtattcaaaa
     7501 atatctgtgg cggtaactcc tataaatcca tatttttgtc ctgatttttc gattacggtc
     7561 gaatttatta atttgccgtt ttttttcata tcgtcaaaca ttttattttc aagtgcaaaa
     7621 agatttcctg ccagagtcgg aatatttgaa ttatttatta tttttgcgca atgttcaaaa
     7681 ctgtggtcaa attcatggtt gccgattgtt tgtgcctccg gtttaaaaaa attgtttaaa
     7741 aattcactcc atatagtata tattttagaa ctgcttccta taaaattatc tcccgttgta
     7801 agcacaaagg aggcggcatc tttgtcttta aaagaagaaa caaattcatc tctggcggtt
     7861 tttaaattcg gtaatttacc gattttcccg tgggcatcgt ttgaaaataa aatcgcaagt
     7921 tcactttcgc cctcttgttt tttattgcga tttatggcaa atttgttgcc gcttccaaac
     7981 gttatttgtc cgttatagtt taatatcctc aacactaaat attaaccctt tctttaaaat
     8041 attaaaactt gcaaaaattt tttttgctat tagaaggcgc gaaaaaatac aaaaatttac
     8101 aaatttctaa tttcattttt catttataag tttattccaa cgctcaaaat caggtctttc
     8161 gcagcacata cagtgaagcc cgccatgaat ttcttttaag gtgccttcta aaatagtttc
     8221 tcctatttta tccttgcatt cgatgaatat tacatcttta atctgtggaa cggcattttt
     8281 taaggctttt tcaaaaattt tattaagatt aattccttct tttttaaaca aagtgcttcc
     8341 gcctgttata taaataaggc ttccgtcagg attttggtgc acaatagcgt tcataaaatt
     8401 caaattgttt tcgcccagga ttccgggaac ttttattgtg ttaagcccat gttcttcaat
     8461 ttctttgata atttgttttg tttctttata tcgttcaata ttttgatatt tttttctgtg
     8521 attatacatt tttaaaattt tatttttcag ttttttattt cttttaacct caggttcttt
     8581 cattaaaaga tttaaaagca tgtcttcgtc agcgaccaaa acatcaggga aaataagtgg
     8641 tcttacagtc atatcaatgt gaaaatctga ctgatgaatt tttaaaatat ttcccttttt
     8701 aatctcaaaa tcttttgata tggttcttcc gtctttatta aaaagggatt tttctccaag
     8761 cagcataaaa gtttcaccgt catttttttt acctataaag aagtttccgc cagcaaagtc
     8821 tgaagagatt tgtttacaat tcaatccaag aaattcggat aattccaaaa gagctttttc
     8881 ggcgtttaaa aataaagagc ctacagttcc ggattccaaa aatactttgt aatcttgaga
     8941 ccatggtttt aatcgggtat ttatgtaatt attaggttta gctttattca gattgacaat
     9001 ttcgttatca gtttgcaaaa taatgtcaaa ataatttgaa catttttgtt gcaactcttg
     9061 tgctacaccg tcaacttctg ctacaggatg aatgtggatt tctttaatcg ggcatgcgaa
     9121 ataaccgcta aagctttgtt gagaaaatgt tttagattgg gttttatatt gtgatgataa
     9181 gttgatttgc atattgaata tctacctttt tgttttataa aaacaggtaa atattttttt
     9241 tgctatcgag cttgcggaaa aattaaaatt tttctcgcaa tctcccttgt tttggattat
     9301 tcggggtgtg gcgaaagtgt ggtttcgcca caccttacga gtttcgcttc gctcactcta
     9361 ccgaaaatcc gctgtccggc ttacacccat accgccggct atctcgcagc cggtatgggt
     9421 gtagcacttc tcaggtcgga tttgaaaaat taagaaaaat tgtcattttt caaatttttc
     9481 cgcatcctca tcaatgcagg gaataatttt aatattcaaa tttatattga agattttcta
     9541 ttgaataaat acagggcgga acttcttttt tgtgataatc tcctgcttcc attgcataag
     9601 aaggcatgtc atagccgaca ctgtagagaa ttgtgcctat gcgttttttc atataataat
     9661 ttctgttatg tttcagaagg gattttgttt ctcttacttt ttctatattt tgcccaaatt
     9721 cagagtttat tcttgcagaa tcttcggggc ttattttttg ccagtaatcg taagtcaaaa
     9781 gcatgttttc ttcatccagg gcaagtgcaa ataaaagaat tgtgttataa atagtttcag
     9841 ggtcgtttgt cctgtttttt aatgctgcaa atgcatattt ttgggctttt tcaaagtctt
     9901 ttttattttg agtttgagaa tatctggttg tatagtattt tactattaac cccaaagcct
     9961 gattgtcaag cacttttatt tcatctttcg gtacgattcc ctttttgttt gttcgataaa
    10021 cgcacatagc ataatctttg aaaagttcgc tgtcttttct tgcttcagcc tgcctgctta
    10081 tctcaaaaca cttatcaagg cgtgtttttt ttgtcccttg agcaaaagcc ggaagcgtaa
    10141 gtgaaataat aaccaataaa actaaaatct ttttcataag ccctctttta caaatattat
    10201 acattaattt gcaatttttg tctaaagcat acaaaaattc gcgccgaatt tgacttttat
    10261 tcgcccataa cctttacaat aactcttttt ttgcgttgtc cgtcaaattc ggcataataa
    10321 atctgctgcc atggtccgaa atcaagctga ccttttgtaa tagggacaat tacctcatgc
    10381 ccgattaaaa ggcttttaag atggctgtcg ccgttggttt cgcctgtgcg gtggtgattg
    10441 taatttatgt tgaagggagc aatttcttca agccacttgt cgatgtcaga aattaagccg
    10501 ctttctgcgt cattaacata aattcccgct gttatgtgca tcgcagaaac aagcaccatg
    10561 ccttcttgta ccccgctttg cgtcacaata tcctgcacct ggtctttaat atttttgtac
    10621 tctctgtgtt tttcggtgtt aaaccacaaa tattctgttt tgaatttcat acaatatact
    10681 ccttttttaa caattataac atgtcatttt atagcaggaa ataggaaata agaaatagat
    10741 agtagcaccc cgcgtcgttg cgaggcttta gccgaagcaa tccaaaaaac attattttaa
    10801 attttttctt agccgctttt ggtggcaaaa gcggcgcaaa agccacgacc tgatctacat
    10861 tcattaactg acactaagct caactcaagc tcgcgaactc gctacatttt tttgttatcc
    10921 gtaaatatta gctaaaaatc cgctcaaaca cactcgcttt aaaattgttt cgctaagtgt
    10981 cagttattca ctccgatatg gtcggggaaa aaccgatatt ccgtcgttgc gaggagtgag
    11041 acgttagccg aacgacgcgg caatccaaaa acggcgggat aataatccca ccatgctcgc
    11101 ttgacctatt tcacttaaaa tagtttaaaa attatttatg acgacttttc atttgtttac
    11161 aaacatcctc agttaaattt gaatttgagc aatataaaac aaccgaaccc caattactca
    11221 ttttacttcc aaggacaaat tttttgaatt ttatataatc acttagggtt tgttcagctt
    11281 tttcataaat aaattttctt cttaaatctg taacaaatac tttatcaacc attttaagtg
    11341 atgtatacat atttgtatct ttagcaaaaa tcaatctcat tgaataatca acattgccac
    11401 caaataatcc tcttgaaata ctatcgtcta aatctaacca atcaggaaca atatccccag
    11461 tttgaacaca aacaatttta ttgaattttc ccttatattc acaatatata tcgtctccat
    11521 ttattggtag agtcattata tatagcctta gcaaaaatcc aacagttagt aatatagcta
    11581 atgttattaa agaaattttt ataacatttt tccaatttcg ttgaggttta ttttctaata
    11641 cattaatttc ttgctcttcc attataatta atccttatgt taaattatgc ttattagtaa
    11701 acaaaatgtg ataaacttgt aaaaagatac gattttcggt gaaaaataaa tacatttttc
    11761 cttgaattta tagtaacact aaatttttca aaagcaataa aaaaagaggg ttctctctga
    11821 actctctttt ttatggtggg ctgtactgga tttgaaccag tgaccccttg cgtgtcgagc
    11881 aagtgcgcta ccactgcgcc aacagcccat gtttattgtt ataatttatt tgaaccagtt
    11941 ttgtctattt ttactgcgct accttcctct cacaaaacct gacatttagt cagcttttgt
    12001 tcggcagagt gccaacagcc ctttcaatag tgagtagtaa gagatgaaaa gtgaaaagaa
    12061 aactattcga cttttcaact aaaataacct ttcagctatt ttaaacttaa aaatatcacg
    12121 atcttctttg ttgggctggc aagcccaacc tactttatga atctttgttt ttaagtttag
    12181 aataaggaga cacccggatt tgaaccgggg ataagagctt tgcaggctcc tgccttacca
    12241 cttggccatg tctccataca ttttgttaat tattaacttt tcaaaaacgg ctccgctcaa
    12301 accgtcctgc tttgaggcaa agcctcaaac tctgccggtt ttcgactcca ctcgatgact
    12361 acgtcatctc gcttgcctct agcctcgccg gcgggcactt ggccatgtct ccctaatgtt
    12421 tttatatcgt attcaataca tgcttgattg tcaagtaaac aacaggttat aaggttggtc
    12481 tgcagagtag aatagttttt agttttttgg attgcttcgt cgctccgtgt ctcgcaatga
    12541 cgtaagcggc ggaagcatta aatccagaaa catttacccc ctcgcaacga caaaaaaaga
    12601 tgcataaatg caccctacta aatgagattc ttcggctttg cctcagaatg acgtgaatta
    12661 attataaatg aacttattca cctattcact tattcactta tttacttatt tgcctattta
    12721 ttcacctttt gtccctttcg ttacgttttc ttaaacatgt tcccatgccc tgttctttta
    12781 gtgtaaaatg gataaactag gaatttttag gtcatggagg ttaatgtgag tctagagttg
    12841 ggagaaaaag tgaacgacaa gttaaaatta tcggaaaatg ccgttaaagt tttagaaaaa
    12901 agatatttaa aaagggataa agaaggcaaa tgcgttgaaa cacccgcgga tatgtttaac
    12961 cgcgtggctt caagtatcgc aaatgccgag cttaaatacg gaaaatctca ggctgaagcc
    13021 gatagtcttt ctaaaaaatt ctatgaagca atcacaaacc tttatttcat gccaaattca
    13081 ccgactttaa tgaatgcagg gcgcgaactg ggacagcttt ctgcttgttt tgttcttcct
    13141 gtcgaagact cattagaagg aatattcgaa acaatcaaaa acacagccct tattcacaaa
    13201 agtggcggcg gaacagggtt ttcattctct cgattaagac ctaaaaatga cgttgtgcgc
    13261 tcaactatgg gcgtatcttc aggtccggtt tcttttatgg aagtctttaa cgcagcaacc
    13321 gaagccgtca aacaaggcgg gacaagacgc ggggctaaca tgggcatctt aagagttgac
    13381 caccccgata ttatcgaatt catccactgc aaaaacgata acaacaaatt aaacaatttc
    13441 aatatctctg ttgcaataac agatgaattt atgaacgcac ttaaaaataa tcaggattat
    13501 aatttgtatc accccaaatc aaagcaagca gtgggaaaac tcagtgcaaa acaagttttt
    13561 gatctgatag ttgacggtgc ttggagaaac ggcgagcccg gaattgtatt tatcgacaaa
    13621 atgaatgatg ataatccgac tccgcttgtg ggcgatatcg aatccacaaa tccttgcggg
    13681 gaagtgcctt tgctggctta tgaagcctgc aatttaggtt caatcaatct gggtttaatg
    13741 ctgaaagaaa cctcaaacgg cttggatgtt gactggaata agcttgaaga aatcacaaaa
    13801 ctttcaatcc attttctcga caacgtaatt accataaaca attatccgct tcctatcatc
    13861 ggcgaaatgg ttcaaaacaa ccgcaaaata gggcttggtg ttatgggatg ggctgatatg
    13921 cttatgaagt tggggctttc ttacagttca aaagaaggta ctgatttagc ttataaagta
    13981 atggaattta tcgattacca ttcaaaaatt gaatctttgg aacttgcaaa atcgcgcggt
    14041 gcttttaaca actttaaagg aagcgtttac gaaagaggca acttcctttc caacaaatac
    14101 aaaggcaaat cagcaggcat gatttccgat gaaatgtggg cagaccttga cgctaaaatc
    14161 aaacagtttg gcataagaaa tgccactaca acatgtattg cacccaccgg tacaatttct
    14221 atgatagcag cagcctcggg cggcgttgag cctctgttcg ggcttgtgtt tttaagaaat
    14281 gtcatggacg gaacagaaat gcttgaaatt aatcctgttt ttgaaaaata cgcaaaagaa
    14341 aacgggtttt actcggaaaa tcttatgaaa gaaatttcca aagacggttc tgtagctcat
    14401 gttaacaatg ttccgcaaaa ggcaaaacag atttttgtaa cggcacatga tgtaacgccg
    14461 gaatggcatg ttcttatgca ggcagcattc caacttcata ccgacaacgc tgtttctaaa
    14521 acaattaact ttgaagaaca tgctacaaga gaagatatag aaaaagccta cattctggcg
    14581 tttgaaaata atttaaaggg aattaccgtt tacagaaata acagcagaac tttccagcct
    14641 atgaatttgg atgctaaaaa aacaccgcaa attgaaatta aacccattga cgaacctaca
    14701 ggtgaactta aaaccgtagt ttgtcctgag tgcggaaatt ctattgaaat ggcagaaggc
    14761 tgttttattt gcttaaactg cggatactcc ggctgttcgt aaaaagtgaa gggtgaagag
    14821 tgaagggtga agagaaaata acaatcatca ccgcaagtta taattatgcc cgatatattg
    14881 aagaggcaat taattcggtt ttcgctcaaa cttacgataa ctgggaattg attattgtcg
    14941 atgacggctc aacggacaat tctgtcgaaa ttattaaaaa atattgtcaa aaagatgaca
    15001 ggattaagct ttttcagcac gaaaacgggc aaaacagagg gcttaaagaa acaattttgc
    15061 tcgggcttga aaacgcaact ggtgattggg ttgcgttttt ggaaagcgat gacgttttca
    15121 agcccgataa tctcttgaaa aaaatcgaag taatcaaaaa caatcctgat acaaatttta
    15181 tttttaattc tgtagaattt atcggaaaag aaaacatgcc ccaatacaga ataaaagatt
    15241 ttgaaaaaac gcaagaaaaa ctttcaaaaa tgatttttcc aaaaaatctg ttttttgatt
    15301 ttatgcaaaa aaatcttata tttacttttt caacagttgc agttaaaaca gaggtgctta
    15361 aaaatgtcga ttttaaatca ccttgtgatg tttttctcga ctggtggctt tggattcact
    15421 tggcttacag aaatgaattt tattacataa atgaagaatt aactcaatgg cgaattcatc
    15481 ccgaaagtta cataaaaaaa tcaaaaaagc ctattttgtt tcagcttgat atctacgggg
    15541 atatttacaa aaagaataag agtgatttaa aagttcttgc atttattttt gcatcagttc
    15601 cagagttaat tttgacaaag gcaaaaggtt ttgttctaaa aactttgcaa ggaaaataag
    15661 cataaattat gcttattaaa acttctctct ttagtcccca atctaatatt aatactcaat
    15721 accctgtctt gccataacac ccatgtcaaa ataatgtttg acaggctgca tttcggtaac
    15781 aaggtgagcg agtgctttaa gctcaggatg agcgtacctt ccggtcaaaa ctatttcaag
    15841 attttccggc ttgttcaaaa gcacttcttt tacttcggac acttttaaca tattcattgc
    15901 aatgcaaata tttatttcat ctaaaatcac aagctggtat tttccgcctt gaatggcttc
    15961 ttttgcaaat tcccagcctt ttttggcttc tttaaaatct tccatacaaa tattatgaga
    16021 ataaaccact ctgtccatgc caaattgcat aacatcaagg tttggaagaa attttgacgc
    16081 agaaacaatc tctccgtagg aagtcgctga atcacctttg gtaaattgta taataagaac
    16141 attccagcca tgccccaaag ctcttagagc aagccccaaa gacgctgtgg ttttgccttt
    16201 gccgtctcct gtatagattt gtatgtaacc gtgttctaac aacttgcctc caaaaaaaga
    16261 tgactattaa accttacaaa cacattataa atttgtttgt aaccctttac atcgattgga
    16321 tgatgtaatt tcatttctcc caaaaaaatt taaataggta tagataataa taactcaaaa
    16381 ccgtttaaaa gtataattat taatttgatt ttttcaaaaa atttttacaa ttattttgga
    16441 tttattcgtg aaaatattgc cattgttaaa cttccgactt tggctttaaa attaaaagtt
    16501 ttacattaat tttggtaaac tttcttaatg aactattgca accatgtagg catgggtatt
    16561 tgcggttatg ttaaaaaaat aatcattttt aataaaacta aacatattgg attaagggct
    16621 taaagagtga aggggtgaaa agtttttatt gtagttgcga agcaacaaaa tgaacttatt
    16681 cacttattta catattcact tttttaaacc tttcacctat taagcttttc agccgttcag
    16741 tcaaaattat tccgcagcgt caccctcttt tttctcagga gctttaactt caacgcccat
    16801 agatttaagc tgtgctgtag cctttggcgc aagtgccgtg tctgcaaaat tttccaaaat
    16861 tgtctggaaa cattcgaccg cttcgtcttt catttgtctt gatttgtaaa gcactccaac
    16921 tttgtaatac aaaggtgcta gtgaaggatc gggggaaacc aacatctggt tatcaagttt
    16981 tattttaata tcaattaaat tttcgttatc tgttggtgaa aaaagtccgt ttgcgtagat
    17041 ttttttggtc aaagtatcaa cgcttgcaga cattgtatcc aagtcttcct gcgtcaaacc
    17101 ttttcctttg cctccctttt ttgccgcaaa ggcagaatca aacgataagg ttaaaataac
    17161 caaagcacct ataataagta acgccaatat ttttttcata agacatccat ccccctaata
    17221 tatatacata tactacataa aaccttgatt tttagcctca attaaatgta tgatatttta
    17281 ggtgaatagg tgaataagta aataagtgaa taagagtact aaagaaaaac tttatcagat
    17341 gtttattttg ggattggagg gggaaacttt aagcggcaat cccaacctta aagctgcgct
    17401 tcaaaagggt ttgggcgggg tgattttttt cactcaaaat attaaaaccg aaaaacaatt
    17461 caaaaattta ataaatgaaa tcaaaaaaga agctttaatt tttccgtttt tgtctataga
    17521 ccaggaaggc ggaagggtag agcgaactga gaacattcac gacggaaaaa aatatttaag
    17581 ccccaaatat gcagctcaaa aaggtgaaga atttttaaaa aaccagacag aacaaattgc
    17641 gtgcgaatta aaagattttg gaattaatct taactttgcg ccctgtgttg atgtgaacac
    17701 aaatccagat aaccctatta taggcgagcg cgccttttcc gataaaaccg atgaggtgat
    17761 aaaatttggc aaaatcgtaa cgcaggagta tttaaaaaac gggattattc cctgccttaa
    17821 gcactacccc ggacacggcg acaccaatca ggacagtcat ttgactcttc ctgaaataaa
    17881 tttgccgttt gaagaaatgg aaaaaattca cataaaacct tttaaggaaa tttctgcgcc
    17941 gatgattatg gcagcgcatt tgcactgtgc ctgttttgat aaagagaaaa tccccacatc
    18001 cctcggcaaa aatgccatag gatatttgcg caaaaatttg ggctttgaag gggttataat
    18061 cacggatgac atggtaatgc aaggtgcgct gggttttgag agtacaaaag cttgtgaaat
    18121 ggctataaag gcgggggtaa atatactttt gtacagaaat tcgtttgatg aaacaattca
    18181 aattatagaa aatcttgcgc aaaaagccct cttagacaaa gaattaaaag aaaatataga
    18241 aatttctttt gaaaaaataa tgaaattaaa aataaaaaat taaagttaaa tttataaaaa
    18301 ccacgtcatt gcgagggagt aaatgtgtcc ggattaatgc ttcgccgttc acgtcgttgc
    18361 gaggagcttg ctttgcaagc gacgcggcaa tctaaaaaat attattttaa attttctttt
    18421 agccgctttt ggatgagtat gtttttcttt cggcactttt tgtcaccaaa aggtacctaa
    18481 agaaaattta tcttacttta tcctaattct ttggattttt ttgctgttct tttgattaat
    18541 tcaaaaaata attcttttgt atttatttcg ttcatgtatt cgacaccgac ttcggtgcag
    18601 ccgccttttg tagcaaccat atttatcata tcctgtgcat taagattgct tgattgcatc
    18661 aattttgcag agcctatcgc ggtttgaatt gccaaaaact tcgatttttc gtaatcaagc
    18721 ccaagttttt ctcctgcaag tgccatttcg tgaattattt tatagaaaaa cgcaggtcct
    18781 gagcctgaaa ttgccgtcaa aacatcaatt tgtgcctctt caacaacaat gcatttgccg
    18841 atatttgaca taaattcctt cacaaaattg acgtcatctt catctgcaaa gtttcctttt
    18901 acaattccgc tcataccttc ttttataaca gcaggagcat tgggcataac tcttataacc
    18961 gcaacttcac cgatttggtc ttctatcgat tttgttttga cgccggcagc aattgaaacg
    19021 acaagcttgc cttgaagttc ttcttttatt tcttgtaaaa cttcttttat aaaatgaggc
    19081 ttgattgcaa ggaatataac atcgcaaatc cttgcaactt cgcaattatc cagtaatata
    19141 tttatcccca gttcggattt ttttacactt gcaagtcctt gagaaatttc agatgctata
    19201 atattatccg accctaaaaa acctgagctc aaaacacctt ttatcatggc gtttgccatt
    19261 tttccgcagc cgataaatcc gactttcaac tctgcttgat taaaaatttt gttcataata
    19321 attaacctta tagttagtga atgattgact aagtgttact tttataataa catcaaaata
    19381 ttaacactaa aatacaggag aataaatagc atgagacgta cacttgaagg aacaaaaata
    19441 aaaagacaaa aagtcagcgg atttagagca agaatggcaa ccgacggcgg cagagaagtc
    19501 ttaaacagac gcagagcaag aggtcgtcat aaattggcgg ttgaaggaag taaaagagca
    19561 taaaagtaaa tagtaaatag taaattgtaa atagagggca cagatatttg atttgtgccc
    19621 cttttaaatt tatttataac ctgattttaa cttttaaatc cccttacttt tctacaattc
    19681 tatttactct ttactattaa cttgtcattg gacacggcac cttatttatg caattattta
    19741 agcatgttga aaaaggattt gagattaaaa aaggattctg catttagtgc tacttatcgc
    19801 ataaggcatt cttacgcttt ttcagggatt gtgctttggg tcggaaaatt aaaaaccgat
    19861 tttcaaactc ctacaaaagc gggctttgtg gtaagcaaaa aaacccacaa gcgagctgtt
    19921 aaaagaaaca ggctcaaacg tcttatgaga gaaagttaca gacttttgct caaagaaggt
    19981 aaaatcgaaa agtctcaaaa atttatgtca ttaatttttg ttggacagga aaaagctttg
    20041 gggcaaagtt ttttgcagat taatgagact gttttgaaat tacttaacgg gataagagaa
    20101 aaatgattga agggcttatt gcaatacaaa ctcaaacaat ggacgaattt tttccgtatc
    20161 ccccttatcc gatgtatcct tcaggactta ttgatgagag caaatccatt tacagatatg
    20221 ctcatccgtc aagcgattgc cctgttttgg taattaaaga tcctatttat gatgaagata
    20281 gaaattatat tccgcccggg cattacagtc ttgttttatc tgatgacagg acgtttttac
    20341 ttttagttca gggggataaa atttttgcca caattcctgt ttttaagctg gaagaaagca
    20401 aagaagaagt agaaaaaatc tacgacaaga aatatcaaaa acagcaggca aaagaaaaga
    20461 aaaaacagga taaaatcaac gcaaagcttg caagaacagg agctccgcct caggaaaaac
    20521 cggtttatat ggaagcaacc attgaatatg acaatatcga acgatactat cttataaagt
    20581 acgaaagagg ttttgtccga gcgtggggcg cgataaaaaa gtaaatagta aatggtaaat
    20641 agaaatgtta aaacagggga caagaggcga agatgccaag aggcgtagta ggataactca
    20701 cgcatctact tatcttatct tcttataaac tattcgactg ttcgactttt caactaaaat
    20761 aactttctca gttcactgct cacggctcac cgttcactca ttttacactt catcgtcaaa
    20821 gtaaacaagt tccaaatgtc cgataattaa agtgtcgccg tcttttattc ctgctttttt
    20881 aagtgcttcc ataactccca tgtttttcat tatattctgc agtcttaaaa cctgctctgt
    20941 atttctggca tcgctgactt gagccaagcg tttaattttt ccgcctacaa ctgcgaatgc
    21001 gtttttatcg actttaacga cttcaaacaa gctgtcatcg ttgttgtatg cgtctaaatc
    21061 ttcttctaca tcaacttcaa aagcaggttt ttctatttca tcgacttttt gcgcgataaa
    21121 aagttttaat tcttcaatat tttcacctgt taccgcagaa atcaaaaaca catcatcaga
    21181 aatttctttg aattttttga agaaattttt tctgtctttg attgaaatca catcaatttt
    21241 gttaagcgca ataatctgat aaagctcaga gagtttttgg gaatgttttt ttaattcaag
    21301 gttgatttta ttgtaatttt cgacaggttt ttccatagtc aaatcaatga tatggacaag
    21361 aaaacggcat ctttcaacgt gtcgcaaaaa ttcgtgtccc aagccgattc cctcgcttgc
    21421 gccttcaata agccctggaa tatccgcaac gacaaaaccg tcaccggatg gttttttcac
    21481 cacccctaag tgtgggacaa gcgttgtgaa aggataatct gcgatttttg gttttgcaga
    21541 acttatttta cttatgaaag tggattttcc tgcattgggc attcctaaaa gccctacatc
    21601 tgctatcaat tttaattcaa gttccaaatc tctttcgata gaaggctcgc cgggttcgca
    21661 aaactgaggt gcgcgttttt gggcggtcgc gaaacgtgcg tttccgcgac cgcctctacc
    21721 gccttttgcc acaagaattt tgtcggaatt ttttgtcaaa tctccgatga tttttcctga
    21781 ttttaagtct ttcaccatag ttccgacagg aacatttatg tacaaatctt ttccgcattt
    21841 tccgtgctgg cttttgcttc tgccgtttac gccgtcttct gctttaaaaa cggatttgta
    21901 tttaaaatcc ataagcgttg acatattttc gtcggctatg aaataaacat cgccgccgtt
    21961 tccgccatcg cctcctgcag gacctccttt atcgacatat ttttctctgc gccaagctac
    22021 catgccgttt ccgccatgtc ctgaaattat ttttgctgta gctttatcta agaattgcat
    22081 ttttgttatt caccatttac catttactat tcatttaaac aaagaattaa gccatttatt
    22141 tgtaatataa tattactatg aacaatgaaa aaaagacact tttcagcaat aaaacagtta
    22201 aaataggcga gaattcgggc tatgataact acataatggc tatagaaaca agctgtgacg
    22261 aaacggcttg cgctgtagtt aaaaacggac gcgaagtttt gtcaaatgtt gttgcctctc
    22321 agattaaaac ccacgcaaaa tacggcggtg ttatccccga aattgctgca agagagcact
    22381 tagaagcaat taatattgta attgaagaag cttttgaaca ggcaaaaatt aagtccgaag
    22441 aaataaccgc ttttgcagga acagtcgggc cgggacttgt cggttcactt cttgttgggc
    22501 ttaatgcggc aaaaaccctt gcgcttgttt gggataaacc ctttatcggg ataaatcacc
    22561 taaatgccca tgtttgcgca aattacatag atactgattt aaaaccgcct tttattgcgc
    22621 ttttggtaag cggcgggcac actcaaatta ttgaggttaa atctcattcc gagcagaaaa
    22681 taatcggcga aaccattgac gatgctgtcg gtgaagctta cgataaagtg gcaaggctta
    22741 tagggcttcc atatccgggt ggtccgcttt tggataaaat ggcaaaagaa ggaaatccca
    22801 acgcatataa gcttcccgaa gcaaaggttg aaggctacga tttcagcttc agcggtctta
    22861 aaacggcggt tttaaggctc gtccaaagcc ttaaaaccgc ctcggacgaa cttcctgtta
    22921 atgatatttg cgcaagcttt caggaaaccg tatcagaaac attgctgaaa aaggttaaaa
    22981 gagctttaga agaaacaggc tataaacaag tcgttttggc aggcggagtt gcagcaaatt
    23041 ccgagataag aagaaagttt tttgaacttg aaaatcaagg ttataaagtt tttgcccccc
    23101 agatgaaata ctgtacagat aacgctgcta tgattgcatc ttgtgcttat ttttccacaa
    23161 acacttttga tgatgtgaat gttgaagtat tttcgagagt ttaattttta aaataaactt
    23221 tgcactcgct tgacaatatt gtaatattag tatattctaa tattacataa ggaggaaata
    23281 tggaaaaata cgcggaaatt ttcaaagcac tggctcatcc cataagactt aaaatagttt
    23341 gcggactttg cggaaaagac gagtgtaatg tttcaaagat ggttgacggt ctaaaaatag
    23401 ctcaaccaac agtttctcag catttaaaca ttttaaaaaa tgcaggaata ataacagggt
    23461 ttaaaaaagg cactcaaatt tgctacaggg tgacaaatga tgaagtaaaa aagattataa
    23521 aatcgttgga gattaaaaag tgaaaatatt aattgtcggc ggaggtgcag caggagcaag
    23581 ctgtgctgcg cgccttagaa gattaagaga agatgcacaa ataacaattt tggaaaaaac
    23641 aggcgaaatc tcaatcgcaa actgcggact gccttacttt tgctctgatg ttatttcaga
    23701 cagggataaa atgcttgttt caaaccctac tgtttttaaa gaacttttaa acattgacgt
    23761 gaggcttcat agtgaagtca ctttaataaa cagattaaac aaaagcgtaa aaatcaacaa
    23821 tgattatgaa atgagctacg acaaacttgt tctcgcactt ggcgcaaact ctataaaacc
    23881 accgattgaa ggtataaatg cccaaaatat tttcaccgtg cgtatgcttg aagatgccga
    23941 caaaataaaa gaatacgtga gcaaaaacga agttaaaaat actgttgtta taggaggcgg
    24001 atttatcggc gttgaaatgg cagaaaattt ggcacattta gggataaaaa cttcgctggt
    24061 tgaacttgca gaccagattt tagcaccttt ggatgccgaa atagcggctt ttgcgcaaaa
    24121 ccaaatgcgc gataatggtg ttgaattaat tctttccgac ggcgttaaaa cttttgaaca
    24181 aaaagaaatt gagcttaatt cgggtaaaaa aataccttat gatttggcaa ttttggcaat
    24241 aggagttcgc cccgaaataa gccttgcaag agagtgcggg cttgacatag gccaaaccgg
    24301 aggtattttg gtcaacgaat ttatgcaaac ctcggatgaa aatatttatg cagcaggcga
    24361 cagtgttgaa attattgatt ttgttacaaa ttcaaaagcc ctaatcccgc ttgcaggacc
    24421 tgcaaacagg caagggcgaa ttatcgccga taacattgca gggcttaatt cgacttacaa
    24481 aaactctcaa ggtacagcgg tagtgaaagt ttttgacctt accgcagcaa gtgtcggaaa
    24541 caatgaaaaa cagcttttga aaaaagaaat cgaatataag aaagtctata tttggggaaa
    24601 ttcgcatgca ggatactatc cgggaagttt tcctttgatg ataaaacttt tattttcgcc
    24661 tgaaggcaaa attttcggcg cacaggctgt cggttttgac atggttgaaa aaagaattga
    24721 tgtaatatct tctgttatgc gatttggagg caaagttcaa gacctgatag acagcgaatt
    24781 gtgctatgca ccgccttatt catctgcaaa agaccttgta aatattgtcg gaatggcagc
    24841 tgataacatt ttgaaaaaac tttcaaaacc tgctttctat gaggatttac aagatgcttt
    24901 tatagtcgat gtcagacccg aaatttcttt taaagttaag acaatagaag gtgcggttaa
    24961 tattcctgta actcagctaa gatggaggat gggcgaaatt cctaaagata aaaaagtcgt
    25021 tcttttttgt gataaaggtt ttaacagcta tgttgcggca agaattttaa tccaaaacgg
    25081 gtttgaaaat gtattgaatt tatcaggcgg ctttcttctt tacaaagaaa ttgccaaaga
    25141 taaaaaggga attgtggctt taaattctaa tccaaacaca accactttgg gcagtgtttt
    25201 aacgcttgat gcctgcggac ttcaatgtcc cggccctatt ttaaaacttt ctgaggcaat
    25261 aaaagagttg aaagaaggcg aaataataga agttttgaca acggattcgg gctttgcgct
    25321 tgatatcgaa ggttggtgcg aaacgacagg aaatacctta ttaaatgttt caagggaaaa
    25381 taaaatgatt aaagcttcga taaaaaaagg agagggcaaa gtaatgattc aacaaactaa
    25441 agacggacaa accattgtag ttttttcaaa cgatttggat aaagttttag cctcttttat
    25501 aatcgcaaac ggggctgtgg caagcggtaa aaaagtgagc atgttcttta ctttttgggg
    25561 gcttaacgtt ttaagaaaat ccggaaatgt aaaaattaaa aagaatttta tagaaaaaat
    25621 gttttctttt atgatgccaa aaggtgctca aaaacttatt ctttcacaaa tgaatatggg
    25681 cgggcttggg actttgatga tgaaatgggt tatgaaaagc aaaaacgttt tttctttgcc
    25741 ggagttaatc aaaaccgcgc agcaaagcgg ggtgaaattt attgcctgta ctatgtcaat
    25801 ggatgttatg gggataaaag aagaagaact aattgacgga gttgaactcg gcggagtcgc
    25861 aaaatatatt gcagaaacaa attctgctaa ttcaaatttg tttatctaaa atttgcaaaa
    25921 gcttttggtt tttcaggttt ttgcaataaa tttgtaggtt gtttaatgtt ttcagcctga
    25981 tttttcccgt ttttcatttt atatatgaat atgtttaaaa gcggaagggc aaagcccaaa
    26041 gccagtgttg aataaataag tcctgatgct tgcataaaat ttattttcca aatattttct
    26101 ttggcaaaat cttttccctt ggcggctatt tccatgtgag attttggtgt aatgtcgttg
    26161 agccagtgta aaataccatt accttttttg ttaatattaa aaagcgtatt catttttttg
    26221 tcaaaaagca tttgccctac accttttgcg acaaacccgc caaggacaag ccagttcaaa
    26281 aagcccaaat aatctcttgt agcagattct tgaagctctg ttttgtcttt tgcggcaaga
    26341 aatcttccga ttaaggttgc tgtataaact gttttaatgg catttccgct tgttacaggt
    26401 ccgctaaatt caagtctttt tataaaatcg gctgggtttt taattttcat aacacccatt
    26461 gcaagagtga aaatccctgc acttgcaagt atttttaatg aagtaagtcc tttattattt
    26521 tttttggttt gcttttgcac ctttgagcca aaattttcgt gccctacaaa agcatcgctt
    26581 ccggtgcgtt ttttggtgat ttgcctgttt acatattggt ttataagccc taatgaagaa
    26641 gctattgcca atgcgcctag agttttggtc gtattcattg atttgagctt tttgccggct
    26701 ttggttaaat ccaaagtttc atttgtaaat atgtttttgc ctgcatcata aatatctctt
    26761 atcaaattgt ttaaagatgt atttaaaatt ttgtcttcaa ctttaacagt cagtgaattg
    26821 cttacgccaa gtgcgtttga aattctgtaa ttcaggattt taagagcatt ttttacatca
    26881 tgtctgcttg cggttttatt gtttataagc tctgagagaa attttataat ctgttcttcg
    26941 cttttggatt cattttttat gtttgcaaaa gccttatcat gccagcggat attatcccaa
    27001 gtgggatgtt cgtaccaatc cacttttccc cattgaatat ctttccaaga aacatttttt
    27061 cttccgtctt gccctgatat attttgtaaa acttctgcta cgtaactttt tgaattttta
    27121 tgcttatccc aagaatgttt taaaatatca aaagttttat ttgtaatcca aaggttgggt
    27181 ttaatgtttg tctttttatc aaaaaatttg ttgtaaaggt atgcaatacc gcttgcaatt
    27241 actcccgaag ataaacaaac cgtaattgtt cctgtggctt ctctgaaaaa agtttcggca
    27301 cctgcatatt tatttctttt tttgctgtca acataagtcc ttggtatcac cattgcaaca
    27361 gtatcaacaa gtgcggcatt taacatcgga tttgtgtcaa tgttcattaa agcctgagta
    27421 atcggcgcat caaaaggccc tttgaattga atattatttt ttgtgtttaa attattatct
    27481 attttcattt tttacttttc tgtttttgtg tgcaaattaa acgctcgaga tttcttaaga
    27541 aatctcgagc gttttttata ttttttatgc tatgcacaac aaaaactacc gcaagatttc
    27601 ttaaaatctt tacagcagca acaagaacct gtttttaaat attgtttttt aagcatgttt
    27661 tcggctttca atttactaca cttatagtgt aaaataaaca gaatgaatct gtcaagtata
    27721 catgttgaca atcttaaaaa atcttggaaa gctgtactac aaaacctgat gttccgtttt
    27781 gaggcgaatt cggtatttga taacccgcat aaacagcggt attttttggc aatttataac
    27841 ttccacctac aatcaaaaac cctgcccaat gttcttcccc tgaataccaa tcacctataa
    27901 gtgaaaactt ttttgtaaca ggctgttcaa ccgcagctat aaaacatgtg tggttaaccc
    27961 cgaacacttg tcttccgcca tagctcatac ccgcagttag tcttgtattt actttaggaa
    28021 ctcttccgct caagtgtgcg taagtccaat ttccgacgcc attaccttcc ataccaatta
    28081 acacctgact tcctattgta aacttaaatt ctctttcggg aaatttttct tttaatcctg
    28141 ctatcggcat tgccgtctta aaccctacgc ccatagaaat gttttgtgta gccggggctc
    28201 cgacattaaa caatatagct cccacttctg tattatgacc aaccccgtaa tctgcataaa
    28261 acgtgttaac aaaatttgca cccggattcc atggttgaaa ttgagcttct tcctgcaaaa
    28321 acacatgccc tttttctgtc acatcggcac ttggaacgtt aaaaaccgtt tgagtcgcaa
    28381 aagcatcatt tttagatatt aaaaacaata ttaaaattaa aataaaccaa tattttttca
    28441 aatttttgtt ccgtttttat actcggacta aaaattataa tagaacaaaa aaattgacag
    28501 gcaacttttt attatctatt taaaatgtta aatcatttta ttttttctgt aaaaatgatt
    28561 gataaaatag caaaaaaata attagacttt tttcttaatc ataaagagga ttgaatgtct
    28621 ttttgtttta taaataaaat aaaaagacat tgataaggag gcagttatgt ttaatttttt
    28681 gaaatacaca tcttatgttt tagtccttgt aggtgctatt aattggggct tgattggtct
    28741 tttcggtttt gatttggtcg caactctttt tggagaaatg agctatgttt caagagtagt
    28801 ttattcgctt gtaggcataa gtgcaataat ttcagctttt accgtgtatt attcttgcaa
    28861 agaaaattag tgtttaaaat acaaaagggt gacgaaaact ttgttttcgt cacccttttg
    28921 tgtaaaaaaa actactcatc caagtcggaa ataaatttgc gccaattttt gtcaaattct
    28981 ttgatgaatt tgtgcgcatc aagcacttca tcttcattta tcggctcagg ctcacctttc
    29041 atctttaaaa ctttatcgcc ttgtgaataa tttttcagtt caaagtcgcc tatcccaagg
    29101 aatgccaccc caaagctttt tccgcattgc cggcaaacaa gcctgaagac aagcatttca
    29161 tcttcttccc tcattatttc gacagaatct tcatcaaaat ccgatttaca ccttgaacag
    29221 caaagttctt taaaaagtgt tgctattttc attttcattt tcctctactc ccatattaac
    29281 ataaatcctt tggttaagtt ttgttaagtg aattgaaaaa caagttaata tttttaacaa
    29341 atgtggaata atatacaggt aagcttattt taggagtcca cagatggaag cttttaacct
    29401 gatactgttc gggttcatcg tctatgctgc attgattgtc gtcccgagca tatgggatga
    29461 atttgtaaca catcactgag tctcaattcc aacttaaaac ctaattagta agacgaggtc
    29521 ttttttgata tgttccgtta agacatgcaa aagtgccctt tgttgtcatg cgtgatataa
    29581 tcgggtgaag agtgagcagt gaagagtgaa gagggaaatg actgatttta agcactacac
    29641 ggttatgaag aaagaggcgg tggatgcatt gggctgtaaa gccgggctta tttacgtcga
    29701 tgcaaccttg ggcggcggcg ggcacagcaa attgattttg gaaaaaattc agccaaaagg
    29761 cagacttatc gcatttgatg ttgacaaaga tgcgatagat gctgcaaaag aaaaacttaa
    29821 aggttacgat aacttaacaa tagtacaaga ttcttataca aatattacgg aagttttaca
    29881 agatttaaaa attgataaga taacaggcgg aattatcttt gatttgggcg catcttacca
    29941 tcagctcaca caggcgcaaa gaggattttc tttttcccaa aatgctccgc ttgatatgag
    30001 attcaataaa gatgcggatt ttagtgctta taatgtggtc aatagctaca gtgaaaagga
    30061 tttgataagg attttcagtg aatacgggca agagcgtttt tcaaaaagaa ttgccaaaaa
    30121 aattgttgag caaagaaagg ttaaacccat tgaaacaaca ggcgaattgg ccgatttaat
    30181 aattgagtgc acgccgagga taaaatcatc cattcatcct gccacaagag tttttcaggc
    30241 aataagaatt gaagttaacc aagagttaac aaatgtaaaa aatactttgg agaaagtgtt
    30301 tcatttatta ggggatggtg ctataattag tgtgataagt tttcattcgc ttgaggacag
    30361 gatagttaaa aatctattta gggagtattc tcaaaagtgc agatgtccta aaaatcagat
    30421 gatttgcacc tgcaagccgc caatgttaga agtgattaca aaaaaaccta tcgttgcaag
    30481 tgaaaaggaa gtaaaagaga atccgccttc cagaagcgcg aaattaagaa tcgccgggag
    30541 ggttttagcc agggagtagg ataattgatt caaagacaag ctcaaattat tgataattat
    30601 ggttcttcac aaggatacta ccagggttat cagagtaatc ctcaatacta taatccgata
    30661 attgagggtt attttccgaa ggagaacaca agaaaagaca tggcaatcag aaaaatcaat
    30721 ttaactttaa ccacactttt gggagttttt atagttttga cttccataag ctactatttt
    30781 tcatcagcaa atgaaatggt cttaaatgac ctaaacagac aaaccgttgt tttaaatgac
    30841 gaaaattcgg atttgcaaaa caaattagac aggcttaaat cttttaataa tgttgacatg
    30901 acaatgcaaa aaaacaacat gcttcaaaaa gcccaacagg taattgaggt cagggcagta
    30961 tcaacagcaa ctccctcaga taaaaaaatt gatgcccaaa aaccttttac atgggctata
    31021 ggatattaat ttgaaagaag aatcaaaggg cgaagtttca aaaagaaact tcgccctttg
    31081 attctaaaaa aaagaaaagg aggctgccgc ctccccagaa ctacaactat cctttaacat
    31141 ctcaaattaa aggtcacttt gccgcaatta ctgcggttgg ttccacaagg aatctctaaa
    31201 tttgttcaca tttgctgctg cgtcaactga caaagttaca ttgtttggag tgaatatgaa
    31261 aaccatttca ctgatttttt gaggttttcc gttaagggct tgtgctgctc cgcaaacaaa
    31321 atcaacagtt ctttgagatt gttctttgtc aagtaaatgc aaatttagaa caatagtctt
    31381 tttctctttt aagttttgaa cgatttgtcc ggcttcaccg aatgagcgag gctcaataac
    31441 cattacttca tagcttttgt aattaggatg agcaacgacc ttcaggtcac tttgtttttc
    31501 actacggtaa ttgcttgaat acataggctc taatgccaaa ttgtcttcaa tagggtattc
    31561 catgccgagc tcgtctgcca tatcttcaaa aacatcgtct ctttgttcaa aaccttgtgt
    31621 taaaaatcca actatagatt taattttgtc tgtaactgtc actaatcttt cctccaaata
    31681 tgttatgtac taaataattt tcttcctatc cttatcattg tggctcctgc ctcacaagcc
    31741 tctttgtaat ctccgctcat tcccatcgat atttctttta attcacaatg atattttttt
    31801 tctaattcat ttcttgtttt tacaacatct tcaaataact cttttaattc cgggcttgtt
    31861 gctccaaacg gtgccatgtt cattaagcct ttgatttcaa ttcctttaag ttcttttatc
    31921 tgttcaaaaa tcgcaaaaag ctctcccttt gaaaacccga atttttgttc ttcttgggcg
    31981 ttattcaact gcaaaagaat tttttgaatt ttcccctctt taattgcctc ttgagaaatc
    32041 gtctttgcaa gttccaaaga atccaccgaa tgtatcaggt caaaaaattt taccgctttt
    32101 cgagctttat tgctttgtaa atgcccgata aggtggaatt ttgagttttg tcttgtgtct
    32161 tgcgaaagtt tttgaatttt ttcaatggcg tagatgactc ttgattcgcc aaagtctctc
    32221 aaacctgcct gataagcttc ttcaatcttg ctttcgtcaa aatactttgt aactgcaatg
    32281 atatttggtt tataaggtgc gatttcttct tgtattttta agacgttttc ttctattgaa
    32341 cccataagtt cctaatccta caagaccttg tcctaaaagc agaacatatt tcgattatat
    32401 tccaactttc atgccaggac aacattttgt ttatttgatt gttttgaccg ttttgatttt
    32461 cccctaaaac catgaaagta catgttttcg ggcgtattta atatttcttc atgtttgtta
    32521 acatacctta ataaattaaa ttatcagtgt taataaggca tgcttgaggc atggtcaaaa
    32581 tattataatt atctgcctaa cataacaagt gtttccatga tatcacttgt tacgccaaac
    32641 actcttgagt ttgcctgtat agcacgttga gccataatca tgtttgagaa ctgttttgat
    32701 atgtcaacgt ttgaagcttc cagtccgcct gatttgattg cacctacacc cattgtgccg
    32761 ccgacggtgt atataggagt accggagttt gctcccattg accagaggtt tccgccttct
    32821 gaaacaagcc cttcagggtt gattacgttg gcaagctgaa tttggaagtt aggcggttgt
    32881 gctacgttag ggttaacgtg aacattgtca tttcttatga taacacctgt tgaggttgta
    32941 tatttaaatc ggaaggtatt atccgattca ttcaattcaa ttgtaatttt atcaccgttt
    33001 gaataagttg cttctatcga accgtcccct gctatactgt agtttgatac tgcaattgca
    33061 gaagtcgtac ttgttacggg agaaacatca acctgataat ccaatatttt acttgtgtat
    33121 tcgtgagtat ctgaattaag tgatgaaagt tctatttgtt caaagaagtt tgacggattt
    33181 gctgttcccg gtccaaagtt aatgttgttt actctgtttg gtggggctat ggtttggttt
    33241 aattcaaatt ttattttacc gccggtagaa gtatcacatt ttattgttag acctctggtt
    33301 gttggagtcc ataacatttt gtcatcgata gccttttgaa ttttatcgca tattttttgt
    33361 attgtattat cagttgcctc taagcttacc ggaactgttt gtgatgttgc agaggtattt
    33421 acctcaataa aaatatctcc tgtggtaatt tgacagtcgt ttaattcaga taatgctttt
    33481 gatgccattg cgttgttagc cgttacagaa gaaacaattt gttggggaac ttttactgtt
    33541 gcgtttgaac tgcttgctgc ataaagctgg ctggcaccta ctactttata accttgagcg
    33601 tttaccatgt cgccgtttgc gtcaaatgta aagttgcctg cttttgtcag aaagaattgt
    33661 ccgcttgaag atcttacggt aaaaaatccg tttccttgaa tcatcatgtc tgtggctttg
    33721 cctgtggctg tccaggttcc agaatccatg tttttagaaa tggttcctac ctgaacaccc
    33781 aaacctattt gaatggggtt tttaccccct gttgttacag taggagagtt cccgcctgta
    33841 agtgtggtag agaaaatatc ctgaaaggtt atattggatt ctttaaatcc gatggtgtta
    33901 atgtttgcaa tgttgtttga aacaacgtta atttgtgttt gagctgctga tataccgccc
    33961 atggttgtgt atagtgcttg tgacattttc tcctcctgtt atatttttct taatttaacc
    34021 tctcacttaa tcccttttta tgatgatgag ccgttattat tgttgtcatt gttatttgtt
    34081 gttgtgtcgt ttgtggtatt attgtttgtc cctgttcccg gttctctgac actttgaacc
    34141 aaagaaagcg ggtattcttt tccgttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34261 nnnnnnntgt tcccggttct ctgacacttt gaaccaaaga aagcgggtat tcttttccgt
    34321 taattgttat acatgaaccg ccgttttcaa atcctgcttc tgtgacggtt cctgttattg
    34381 tttggcgcgg attatcaggg tctgttatag taacttcttt tcctacaagt gcaagtgttt
    34441 gcataatgta gttgtttgtt gccatatttt ctttgagttc ctgagttgca gaaacttgag
    34501 tgaattgagc ctgttgtgct aaaaattctt tattatccat aggtgaaaga gggtcctgat
    34561 actggagttc ttttagcatt agcattaaga aagtatcggc atcaaattgg cttgtgccgg
    34621 ttgtttttgg caaagatgcc tgatattgag cagttgcatt ttgcatatct gttatttggt
    34681 tttgtactga tgctgccatt ttattttctc cccccgtttt taatttttaa accttgtaat
    34741 caactcttcc ggtatgtttt tccgagatta ctgtttcttc ggcattttct tctgaaatat
    34801 cagaatcttc cgaagagtcc aaagagttgt tttgaacgtt ttgttctttg ttgtcggctt
    34861 tttgtgaatc tttttgttct tgttcatctt gcatagtttg ctgctgatat tcaaaattga
    34921 gcatttcgtt ggattgtttt tctatgtttt caacttttac attgacttca ttaatctgaa
    34981 caccttgtgc tccgagcgag tttttaagcc cttccaaatt cttttccaaa agttctttga
    35041 cttgaacgtt ttcggcggtc attcttgcta taagtccgtc tttgccgtta attaattcca
    35101 aattgatttt acctaaattt tcaggcttaa ggactattgt tacctttgtt gagccttcgt
    35161 ctttggcctc tgtaagtttt gtgtgaattt gtgccaaaat gtcattttta gaaatttctt
    35221 ttggagattg aatgctttct attgttttac taaaacttgc ttttgcatcg gttatgttta
    35281 aattttgatt gttttgttgg tttgaatcca ttaaagattt aattccgttt tcttgtgcgc
    35341 tttgtttatt caaaaggttg tctgatgagg cgttttgttc aacactgaca actcttgcat
    35401 ttgttgcatc aagcatttct tgagttaaaa ttgttttctg taagacatct tttattgatt
    35461 gtttttgttc ggggttttcg tcactttttg tttttacatc aatatttgct tgtattacag
    35521 gtgtttttgc cttaatgtct gcattaattt gttcaatatc tgtttgagcg tttttcggaa
    35581 tttgatttgt aatttctggt gttgtattgt cctgagaaat attttctata tttatattaa
    35641 gcagaacctg ttgagtttgc ggctgtgttt gaacttttgt tttgtcagtc acaggctgta
    35701 tttgagtttg tgtcggaatt tgaggttgaa cttgattttg ggcagtagcg atttgtaaat
    35761 ttttcaaaag tatatcggca gcctcggaag ccacaggtac agttattgaa gtttttgact
    35821 gctcggttga ttgtgcttga tttttttgtt ccgggtctgc ttgaacttta ttttcggctt
    35881 ctgctgtttt gccctcatcg taagattctt tttgctgggt tttgctttct tgaattttgt
    35941 cttgttttga atcttgagaa ggtttgtctt ttggaagatt tgtattttct tcaattttat
    36001 tttctttttc actatttttt tgtgaattat ctttcaaagc tgtattttca gatttgtctt
    36061 cttctttttg ctcgtatttg tctgcttttt tttgataatc aactttttta tcttcagagt
    36121 taaatttgtt ttcttctttt gtataattct tgcttacttt atcaaaaaca ttttcaaaag
    36181 cttcagactt atcggtgtaa gttttttttg aactttcact ttttgttttt atttgagtta
    36241 gtaaatctgt tattgctgtt gccatatttc ctcgttttat gctgtcattt tttggtaacg
    36301 tgttgatgct atatcatcta tttcttttcc ttgaaggtat tcgtagtgtt ggtagaattt
    36361 cttttcttgt ttttctttga gtttttccag tatttttact tctttaagtg cttctgtcac
    36421 ttcttgttgt tttgcgttta atactatttg aatgttttta atcacttctt cttgaatttt
    36481 tatgttgttt atcatttttg agaaaaagtt tttgtggttt gtaatttcta aaatatcaag
    36541 ttcagcgttg ttttcgtaga ttttttccaa attctttttt gttaattctt tcttgtttaa
    36601 aaggtcttca agaacttgaa gctgcatatt cagaagactt actacttttg ccatttcaag
    36661 ctgtttttct tcaaggactt tttgtcgcat gccaagaaca gtttgcaatc tgaacttata
    36721 ttttttcaaa aaatacctct tcccgcatag attttataag acttttcttt tcttatatat
    36781 aatatatgcc taaacacagg gtacaacatg attggcgggt taatcatcat ctgttttatg
    36841 taaatttctt aaaaaaatca tttatttgat ttagaaattc ttttttaata tgatatttca
    36901 gggaagtttt ttgagaggaa gtatgatttt attttttata tcattttttt tggtttttgt
    36961 gtcgtcttat tttttgcgcc taaaaaaaac gttttaggct taatttattt gtttttaata
    37021 gcttttgctc aaattgtgct gacttttgaa gtattgtcat tatttagcgc aataaaaccg
    37081 tttcaagctt taggtttaaa tattttattt ttaattttta gtacaagctt ttggtttaaa
    37141 aggtcaaagc cgctttggag tttagattta agcgacttta aaaataaaat ttttaactct
    37201 tttaaattag ataaatcttt aatctggctt tttgtcggat tttgcatctt tatttttgta
    37261 agtttagtct tgtgttttgt atcgccgata tcaagtgccg atgcttctca ttatcacctt
    37321 gcaagaagct tattttgggt tttgcagggc agtttaaatc attttgaaac cgcagatata
    37381 agaaatttat gttttcctat aaattctgaa atattatatt cctggatttt gctttttgct
    37441 aaaaaggatg tgtttttagg ttttttctcg tttgtcgggt atgttttatc tgttgtttca
    37501 atttattcaa cacttggctt aatgggattt tgtacaagaa aaaaactttg ggcaattttt
    37561 atattatctt cattttcttc tgttatcgtt caagtttcag gcacagaaac agatgtaatc
    37621 gttgctgggc taattacttc aagcattttt ctttattggc atgctcttaa gtatgaccaa
    37681 aaaatgccga tttttatgtc ttcgcttgct tatgcgcttg ccatagggac taaaacccct
    37741 gctgtaatgg caattcctgg ggtggggttg ttgtttttgt ttttgtcgat aaaatttaga
    37801 gccaaagaat tttataaacc cttgctactg tttttaggct ttgggctttt gaattttata
    37861 atttttgcat cttacaatta tgtccttaat tttattcatt ttcaaaatcc ggtttcttct
    37921 aagggattgt tgatgacgac taaaaattat tacggtgtta aagcaatccc tgcaagtttt
    37981 ataaaatata ttttcttatt ttttgatttt accggattca ggtggtctga ttatgtcggg
    38041 caatatttta ctgattttaa aaatattttg ctaaattttc tacatctttc atatattaaa
    38101 gacggacttt attcaatgga tacggggcct aatagaacct tgcttgagcc tgtaatgggt
    38161 gcagggattt taggtttttt ggtttttttg ccttgttggt tttggtcttt aatcagacca
    38221 ttgtttaagc ctaaatctaa aaaaggaatc cttttattta tttttggctt aatacttgtc
    38281 ataaatattt cattaatgtc ttatttaatt gtatttatga tatacagcat aaggttttta
    38341 gccactttta tcataatttc tgcaccggtt cttgtttttt cttattttaa aagaaaaaat
    38401 gtttttaagt ttgttatagt tgtatttgcg cttttttatc ttattcttgt ttcgactcat
    38461 ctttgggcaa gaccttttat aaaaatgtca aagctttttt ataaacatcc ttcaatttct
    38521 tatttaagag aagcctttaa atgcagaggg gaaaacgatt ttaatgctta ttcaaatgcg
    38581 acttgtgtac ttagggatgt tatcaaaaat aaaatttccg aggaaaataa aatattagcc
    38641 ctgtttagtt cttcacaaga tatttttacg ataaaaagac tcgaattttc aggttataaa
    38701 atagattttg caagagcgga aaacattaaa aatattgatt taaaaaagta taatataata
    38761 attatcgatt ttaacgatat taaagcaact aatattaccg aatatgaaaa aagaaaagac
    38821 gaaactaaaa ttgagaataa taaaattatt acaaaagaag gaaatatttt cccttgcatt
    38881 tataacttta atgaaacctt accaaaaagc ttattgaatg ataaagaaaa aatatatcca
    38941 tatgttagtc aatgcattat ttcaaaagag tttattaaag aaaacaaatt atatatttta
    39001 tataaagtcg cacgtccgtt atcagaaaat cgctttgaag gttttattat gcttaaaaat
    39061 caaaataatc ccttaatttt gcataacaat tttaaaaagt aggatttaaa ctctgccgta
    39121 aatatcttcg tagcgcacaa tatcttcttc aatcagtgtt tcgcctaatt gcacttcaac
    39181 aatttcaaga tcttctttaa aaggattttg aagcgagtga acagctttaa gcggaatatc
    39241 aatgctgtgc ccgggtgtta aaatatgttc tttgccgtca agaataactt tagcttttcc
    39301 tgaaagcact acccagtgtt cgcttctatg gttgtgcgat tgcacagata atttttgatc
    39361 aatatttacg tggatttttt tggttaaaaa tcctattcct tgcgccagta cagtataaaa
    39421 tccccaaggg cgatagactg ttttgtgcac aaggtgagtt gtatcgttta tttttttgag
    39481 tttgtcaaaa attttcttga cgtcttgggt tttatctttt ttgcaggcaa gaattgcgtc
    39541 ttctgtttct acgataactg tatcttccaa gcctattgta gcaaccagct ttgaagatga
    39601 atacataaga gaattttttg aattttcatc aattacgttg ccgataatta cgttattgtt
    39661 tttatctttt tgacctacat cataaatcga ttgccaggag cctaaatcgt tccagtcgct
    39721 ttcaagtttt acaagcgcga ttttgtcgga tttttccata atggcataat ctattgatat
    39781 gcttggcatt ttatcaaaca gattatattc gattttatcg gaagatgaaa agtcaaattt
    39841 tttcatcact gaataaattt caggtgcaag tttttctatt tctttaagca tcgttgaggc
    39901 tttgaacatg aaaattccgc tgttccaaaa gtaagttttt gcatccaaat atttttgtgc
    39961 ggttttggca tcgggttttt ccacaaactc tttcacttta aacccgtcca aaagagggtg
    40021 ccttaaggta tttatgtagc cgtagcctgt ttcagggtaa ttgggttcta tcccgaaggt
    40081 tacgatgtag ccttctttgg caagcttttc accctttttg actgtttgtg taaatttttt
    40141 ggtgttttta atcaaatgat ctgatggaac aacaattatt acagggtctt tttcttgttg
    40201 ctgaatgaat tttgttgcaa gcgcaatagc cggtgccgta tttttagaaa gtggctctga
    40261 taaaacaaca ggatttttgg caaggctttc aagctggtat tttacacttg aaacatgttt
    40321 tgtgtttgta atgcttatta tgtttttcga gggcataaag ttttgaagcc gttcaaaagt
    40381 tgcctgtaaa agactttcgt ttgaagttaa atttaaaagc tgcttagggt aaagctctct
    40441 agataatggc caaagtctgc ttcccgagcc gcctgctaaa attattccgt acattttaaa
    40501 ccctcttttt tatcaactgt aaatatcata caataaaaaa cttttctttg taaaatcagc
    40561 tttttatccc ttctgattta cctccccaaa tgacctattt ttgtatcttt tctgtaacat
    40621 tttgttaaaa aataacaatt ttttcctttt agatttatta tatagaggac aaggaaaagt
    40681 caaaaatata caagtgttaa
//
LOCUS       DAOQAU010000010        37288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1011_length_37288_cov_7.573550, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000010 DAOQAU010000000
VERSION     DAOQAU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 37288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37288
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1011_length_37288_cov_7.573550"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKI94_02945"
     CDS             <1..357
                     /locus_tag="PKI94_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25726.1"
                     /translation="NLFLGEKLSEYHTGFRGFTKEVLQKLPLEECSDDFIFDNQSLAL
                     LFYYRYKIGELSCPTKYFKEASSINFFRSCKYGLGVLAVSLQYRLAKMGLKSKIFKTT
                     AKKLDLDAKLEYFYKN"
     gene            423..1736
                     /locus_tag="PKI94_02950"
     CDS             423..1736
                     /locus_tag="PKI94_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25727.1"
                     /translation="MSYIGRVSFKIANYFSTITSKTPVTKRTFQAQRDTFCSTNSQIE
                     DDFELKITDDPNLLEKKIKQKILSANINGETLTGWDAEIRSEATAAIYKKNFFDLLSQ
                     KAQTVKDEKIITHINQIFNEIFCKSKITSEEHIQNFWYFRDLLDAYNMSEGLYSKVGK
                     TDEYYKIYTKNEDEDYQKCLINLIFSDKNLLYKILEENPNKNNSLAKWLVNFQNDSRF
                     ESLKYYIKNYSLSQKDMTNYMYEKYYLSQIPENTKKLCEKISAEFGIKMFLHNNYDKE
                     NLNIIYDELSNWKKASQGKALFPHVIDLSTIDPDYIKEKSPSAAYFSTAYKFICVNGN
                     RPLNMQKGSLRHEMTHLNDLKPNDDSYFENLLENRNKFQQEFANAGIDQEYIDYAFKK
                     DASEFKAVATEGDYSKYSDEFKDLLVNLGLPKWVFNMTPVGKMEK"
     gene            1820..2773
                     /gene="fba"
                     /locus_tag="PKI94_02955"
     CDS             1820..2773
                     /gene="fba"
                     /locus_tag="PKI94_02955"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNW25728.1"
                     /translation="MSERKLVGTNEMFKKALEGKYAIGAYNVNNMEILQAIAEAAEET
                     RSPIILQVSAGARKYANQTYLIKLVEAALATTTVPIALHLDHGADFEICKSCIDGGFS
                     SVMIDGSRFPLEENIALTKQVVEYAHARGVSVEAELGKLAGVEDDVNVSAADAKYTNV
                     QEAVKFVTETGCDSLAIAIGTSHGAYKFAGEARLDFERLAQIKAALKEAGHGDYPIVL
                     HGSSSVPKYLVEKCNQFGGQLPNAQGVPEEMLSFASKNGVAKINIDTDLRLAMTATIR
                     EFFVTEPAKFDPREYMGPGRAAVKELVKHKMQVLGTAGHAE"
     gene            complement(2871..3044)
                     /gene="rpsU"
                     /locus_tag="PKI94_02960"
     CDS             complement(2871..3044)
                     /gene="rpsU"
                     /locus_tag="PKI94_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNW25729.1"
                     /translation="MPEIKVGDNESIESALRRFKKKIQKAGILSEIKRRERYEKPSVK
                     RKRKSEAARKRKV"
     gene            complement(3236..4546)
                     /gene="fliI"
                     /locus_tag="PKI94_02965"
     CDS             complement(3236..4546)
                     /gene="fliI"
                     /locus_tag="PKI94_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNW25730.1"
                     /translation="MINFDKYSNIIQQTCTMREIGKVTEIIGLVIESDGPQSSIGDLC
                     YIYRNYNEPPVWAEVVGFRQSKILLMPLGSMEGLQPGAIVVNTGGAMKIKVGEQLLGR
                     VLDGLGRPLDNLGEINSQTLYSTSSEGINPLKRRRITEALSLGIKSVDGFSTVGKGQR
                     MGIFAGSGVGKSTALAMMAKNTSADMNVIALVGERGREVREFIEKTLGVEGMKRTIVV
                     AATSEQPSLVKIKAAQVATAIAEYFRDTGRDVLFMLDSITRISMAQREVGLAIGEPPA
                     TRGYTPSVFAQMPKLLERAGSNEKGTMTGLYTVLVEGDDFNEPISDTARSILDGHILL
                     SRDLAHRNHYPAVDVLESISRVMPDVTSKEHQQAAGNLRNLLAVYKKNEDLINIGAYV
                     KGSDPKCDKAIDMMDKINSFLQQEVGEKFSYEEIVDELIKLSQM"
     gene            complement(4632..5396)
                     /locus_tag="PKI94_02970"
     CDS             complement(4632..5396)
                     /locus_tag="PKI94_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNW25731.1"
                     /translation="MTRIKSDNIKLGGSFEVPIEQSLSSTKIKEALSKEQEIVLQGET
                     KARQIIEDSQRQANEIIENAKVQALNEVEAIHQQAFQEGFEAGRIEGTEKIKEELSQK
                     ILAVDNFAQSTFDIKKNIVKSAHSDIIQLVLEISEKICMKQFDLDKNILKEITQGAIQ
                     SLKDKEDIKIIVNPVMAEQIRGVSDEIKEQIPQLSSIKIVEDNSVSPDGTIVESPLSR
                     VDCRVKSQINEIAEKLMTQVNFEISETLSAENENLI"
     gene            complement(5389..6414)
                     /gene="fliG"
                     /locus_tag="PKI94_02975"
     CDS             complement(5389..6414)
                     /gene="fliG"
                     /locus_tag="PKI94_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNW25732.1"
                     /translation="MLPDLQIQSMTPTQKVAALLIVLGPATASEVLKNITDDDLLEQI
                     TLDIASLNKVPTEILTGILEEFKAIFQASNYISAGGMDYAKQLLEKAYGGDAAKKILD
                     RLVVLMNSNPFQFFNEADPGQLATSFQNENPQLIALIMAYLKPEHSAQVLNYLPPEVQ
                     AQVAMKIADMDTTNPEILSEIEKIVESKFSSVVVQDFSKAGGVEALANILNRADRATE
                     RNVIELLEVQSPQLAEEVRELMFVFEDIVNLEDRAIQRILREVDTKDLAMSLKGTKED
                     VKEKVFKNMSERAQQMLRDEMEYMGPVRAREVQEIQTKIVGVIRTLEVAGEIIIAREN
                     VEDELID"
     gene            complement(6417..7985)
                     /gene="fliF"
                     /locus_tag="PKI94_02980"
     CDS             complement(6417..7985)
                     /gene="fliF"
                     /locus_tag="PKI94_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNW25733.1"
                     /translation="MNNYFKLVIDDIRKLWNGLDIAQKFGMLTLTIVTFVVATYFIIK
                     SMEPNWAVLYTDLSEPDAVSVVENLKKNGYAYKIADDRKTILVQADKKDELRVFVAEN
                     DLIKNSSPGFELLDELPLGSTDFKNKLTKQRIFQGELTRSIENMNGIKSVRIQIADPE
                     RSIFEDEDEEPTASVMLILNPGYKLRGSQVKAIKNLVAYAVPRLTPDRVFITDQNGNN
                     LGDENGKNSNDMETFKSNFEKQTSEKVITVLEKIMGKGNATVQVSADINFDSAKSTIE
                     SYIPLNDKGEGVLTTSQNETENYANPGQGPAPAAAGGNKNLNYSKEKNSTNYSVSKEI
                     KQVIYAPGTVKRMTIAVAVNKILTPKEKEEIQNLVLSASGADYNRGDVITVSSMQFES
                     IAEDKAAQDQMEKDLAQENNIEFWTTKIAPLLIVLILGAGALIVVKSLTKGIGAGGGG
                     VNVANETYNSKMFEAQLPTIKEEPTDLIENEALPQIEAHLDPELERVRMELNDTILAD
                     PSEAARLLTSFIKD"
     gene            complement(8135..8599)
                     /gene="fliE"
                     /locus_tag="PKI94_02985"
     CDS             complement(8135..8599)
                     /gene="fliE"
                     /locus_tag="PKI94_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNW25734.1"
                     /translation="MNFVGPIEGMNFSPVAEYNNYLKGSASFEVNSDLSFETILDRQK
                     DTLRQKNVVQKSQDAVNSDFENVLSSSRIKQNSAANFLNSFGNALGGGLNSVDQSTKT
                     FERAQEAMAMGENVSVHDVMIAAEKANLSMQMAIQLRNKIITAYNEINNVKV"
     gene            complement(8687..9193)
                     /gene="flgC"
                     /locus_tag="PKI94_02990"
     CDS             complement(8687..9193)
                     /gene="flgC"
                     /locus_tag="PKI94_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNW25735.1"
                     /translation="MSFDVFDVAASGMNAQRVRMDTISSNIANINTTRNPDGSVGPYI
                     KKQVSFKAIYDEKMSQGASNFSNNSTSATFNPATGTLKINSGVSMNYGQMSSGVAIDG
                     IVEGKNAVKTVYDPSHPDANADGYVTLPNINVVEEMVGMVEASRAYEANSVAAENVKN
                     MISAAMKI"
     gene            complement(9265..9744)
                     /locus_tag="PKI94_02995"
     CDS             complement(9265..9744)
                     /locus_tag="PKI94_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25736.1"
                     /translation="MDLISTRAIDITKFALDGLMERQKAITANTANIMTPGYVRKEVS
                     FENQLKEMVEKDDLRTYIKEQNSIKYNPSSIDEVTSFGQPQNLTIQKANYLQSDIYSR
                     FEPQITDDTMSGAAQDGNNVVIEKEMMDMAKVGTQYTILSNLEQRALKNVLDVIKGQ"
     gene            complement(9748..11007)
                     /locus_tag="PKI94_03000"
     CDS             complement(9748..11007)
                     /locus_tag="PKI94_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW25737.1"
                     /translation="MHKNKLKDLIYIQDNKPCNEQTIKGLFRAIVEPILDASVESVIM
                     CRLEDKSKKDFNSILKRLEYSSAEVYDFSDKPLSEKFDYVLKEKIWDKTEFIYVLAQR
                     YGAVLVFDYEQGEIEGYAQIYILYNSKNLSDAFNMINSNSTKDLNLYQDKWHPDRRDN
                     DHLNSSIRKMVENLNETNQEVLISEIEKEEIQNSSEDMMKMNLVLKNSSSVAHEVRNQ
                     LSICDLYSNIIQKHLNKISTEESILNSIENALNSIQKSVKLANNSLLELKSFKEASLK
                     SVELKPLIQNVVELGKIYAHEKDIEIKLNIGEDILILADENKLCSVLINMIKNAIESI
                     DKKGKITIESRNKNDKIEILVSNTGEPIKKDLQNKIFETGFTTKKTGSGLGLGICKKS
                     IEEQRGFLRLIKSDEVSTDFEIVLNKG"
     gene            complement(11087..11800)
                     /locus_tag="PKI94_03005"
     CDS             complement(11087..11800)
                     /locus_tag="PKI94_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW25738.1"
                     /translation="MRRAVEFKKKARVLLIDDNYEHLAGIREIINLESQFEVVATSTS
                     ANIGISLVKKHRPDIVLMDINMPEKDGLQAIQEIEKLNLGVRCVALSGYDDSDLIFRA
                     MKLGAKGYVLKTMASAQLIYAIEEVAAGKVYLPAALSSRFFEYFQRSFKEETTIISDE
                     ENLLSYLTQREEEVLDLLTQGITYKGVAGKLFISETTVKTHVNNIFQKLQVNDRTQAV
                     LYAINNGFTNKRKMQLTTV"
     gene            complement(12190..12981)
                     /locus_tag="PKI94_03010"
     CDS             complement(12190..12981)
                     /locus_tag="PKI94_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25739.1"
                     /translation="MKVFFNGFFTKVSVLTRKMVGSVQSDLVRSPNQDVFQQGLKGFS
                     KEIGVAKKALGKRKFSDTDSFEIYDRLRMENVGVLNKLIKEKGQNGQKYRFSIEDLKE
                     MAKVEPSKSLRLEELADAVKAGKEKRFSAQQIITFAKLDDVEYSRAQSLMDFKNLSGE
                     DLILLTKQDLAPQKIESLKVAQEALANKLTSESVRNCLTEESINMQELLPGIKRYYEA
                     SNGLIEKILIRKDGKFNRAEYYYPNENIEKVLLDKGGNILTHAQI"
     gene            13124..14206
                     /locus_tag="PKI94_03015"
     CDS             13124..14206
                     /locus_tag="PKI94_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNW25740.1"
                     /translation="MEILIFIAGFVLGAGLVFAIFALTQRKAEDAKEELISRMQLQFE
                     NLTNKIFKESSQEFSEQNKAKLEEFFSKFRQRIEDFEKRTEFNFKTEAEKLTQFDSNV
                     KTFIEAGNKISQDTTSLATMMKGDNRRQGSWGEIVLERVLEASGLREGQEYELQKGMT
                     DGRPDATINLPEGKKVYIDAKTSLASWDAYVNSEDETEKEENLKEFLESTKAHITGLS
                     KRPYSLSDSSPDYVLMFIPIESCYSLLFCEDSKLWDLAWKNNVMPVSPSTLLAALKII
                     NNFYKIEKQNKNALEISRICTKMLDKFADMLKDVLDARKKISSALTRLNGHDSIIRNI
                     EKLQELGTTINKQIPEIGDDVLEEIS"
     gene            complement(14218..15699)
                     /locus_tag="PKI94_03020"
     CDS             complement(14218..15699)
                     /locus_tag="PKI94_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25741.1"
                     /translation="MRIIFNRINFFKPYFTSHERNANTNHVLPSDNSSNLTGQKGKRK
                     LNFKKTLFLERISKQKEKRHIKKLLIKKLSEQNIENLFKSNDIDYTKIFQKLILACEN
                     IKGQIKEINIRIIEAPLNNIFTEILFKNHNSKIILMNKNGEILSEGQIKTERLKKGSK
                     KLIKTLHDFRNNTESKTISLLCNGLEEILHERIIFRNNVGEIIESFYLQKSSVAGVYN
                     LNYKDKQNKLINYCKALKDKNGNVIIKKNIRGNNNVKTQYYYIKTPSGSRKQLYRIID
                     ENGETLLNKTNIFEVINDNKSFTNINGRKYTTEIVDNSVIINNINTGEVLSYNIDDII
                     ENKRAETIAFFKKINAEDIITMIKNKTKFFYVADDYLSGYSPTANVISCRDNLFSFGH
                     ELGHTKVTSEILNDEKLLKIFSEEQLLFKNASTLLQQNHLNYFTETSDRTPKSNLHEL
                     IAETNAILNTYQSNSCFGIRAHYLQQYFPKTIARICDLIGNNK"
     gene            complement(15866..16312)
                     /locus_tag="PKI94_03025"
     CDS             complement(15866..16312)
                     /locus_tag="PKI94_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNW25742.1"
                     /translation="MKKILMILGIIIAICVTSAVTMAFAPKKNLKPSDYSIGITYEQA
                     VKDKKPFIALFYADWCSYCIRFMPKYKLISDIYKDKYNFVMINVENPKYQKLVEDYSI
                     SGFPSIFIIDKQIDNRIPLYNGIYDDLGKLRGELDRYLKIRSMIKK"
     gene            complement(16377..17612)
                     /gene="glgC"
                     /locus_tag="PKI94_03030"
     CDS             complement(16377..17612)
                     /gene="glgC"
                     /locus_tag="PKI94_03030"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNW25743.1"
                     /translation="MANEDVLVMILAGGEGKRLHPLTRDRAKPAVPFGGRYRIIDFVL
                     NNFINSGFFKIKVLTQYKSDSLNKHITRGWALSPFLNQFVDLVPAQMRTGGEWYKGTA
                     DAVFQNINHIHDLNPDYVCIFGGDHIYKMDVSQMLAFHKKTGSDLTISAIPIPVSEAS
                     EFGIMEVDENWKLINFVEKPKSKPKTIPNNPNMCLASMGNYIFNKDILLNAINQDAKT
                     KVSNHDFGKNVIPTLLKEKKKIYVYNFAQNKVPGMSDSEKGYWRDVGSIDAYWQTNMD
                     LLDYCPELNLYSKEWPIRTFNYNYPPAKFIWEEGDRVGMATNSMVAEGCIISGGSISR
                     CILSPKVRINSFSQVSESILMENVSIGRHSEIRRAIIDKNVEIPPYTKIGFNKQEDEK
                     RGFHVSPGGVTVVPKGSKL"
     gene            complement(17663..18436)
                     /locus_tag="PKI94_03035"
     CDS             complement(17663..18436)
                     /locus_tag="PKI94_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNW25744.1"
                     /translation="MNNFFKCILSWFFVFIAVFLSSQNAFCKTISKDFQIETKDARVI
                     KANLSYLKIENAKKYPTVLLLHSLGYSSENWGNLADMLVNSGYAVIKMDLRGHGKSVY
                     DANLKQRSWQYFTNKVYQKFPSDVVQMLSEIDKQSKKTDLNRLVIIGADIGANTAILV
                     SKNLSVKPKALVLISPTMTFKGLYVPIALSEIGYVPVLAITSQKDSFCLQEQQKLSRF
                     SQGAFYVKNYPNGGMGMLMIKVNPAMSSDIVKWVASFVK"
     assembly_gap    18460..18559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18700..19602
                     /locus_tag="PKI94_03040"
     CDS             18700..19602
                     /locus_tag="PKI94_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25745.1"
                     /translation="MIVIDADIYSLANRFGIAQSKFTQYANKSIDEIIEAEAAQGNSA
                     AAKFSREVLKDSSALVELFKLSSVGNRFAILQNLSEQELEKLLPFLEKQDLIIGLNYF
                     DKAKLLELMGRLPAEQLVKYVLEMFPPKEIMKLMPAESMDKFLTLPDLDKNLVLKHLM
                     QMKPEFLAQMIESVTGKPAKSLNPQELVKEIASLPQEKYKEALVNIPEDQKRKFILAM
                     IEEKPKLLEKFPAEGFVKVLNEKEKPDLIKAAKVIEPEFLVEMLKELPQDLLAVIVTQ
                     INPEIFAQVLIKSYQDILRSIIST"
     gene            complement(19599..20141)
                     /locus_tag="PKI94_03045"
     CDS             complement(19599..20141)
                     /locus_tag="PKI94_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNW25746.1"
                     /translation="MNKFFESLDLIYKKITARTEKKVARFLLKNSMTLSVAESCTGGL
                     LSSRLTDIAGSSAYTKENYITYANEAKIKLLSVTPQTIEKYGAVSEECALEMVKGLFE
                     KTGCDISIATTGIAGPGGINENKDAGLLFVAIKSNFSSAVRKFELNPKISRKKMKFLF
                     TQKALEFLLEFLSEKSNIKY"
     gene            20265..24749
                     /locus_tag="PKI94_03050"
     CDS             20265..24749
                     /locus_tag="PKI94_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25747.1"
                     /translation="MGLISSISDFLSEKAGKVSKKILSKGLAKVKSDFVRSPINDCFE
                     STSQEVCSNVNKIQIDLKNFNLKKFLKSLVDENGKRLIDKAKISVMKSNSKYYGTNEA
                     LPAFIKNLVELDVFDQKQLAEIIKELGNSRDLRQHAEVLKELVKMKALTKNADSEILE
                     WFIKQKTNYALSFDNVQPRKNLLKILNSIEEMYPDTEKSQRLVNRLGKSGMNEILRYF
                     DDEFCLPQYEELLKTFSKNKDINSSAMTRTFRHTGTSRGFEKQEMINAKTLFVNLFND
                     SEIVKSKLKGNLADILCSIYDDKQAKGKMELLSEIFKHPNSENLNSNSINNLVARCFN
                     EEAMASNLDFAKSLLNSKRLEPRSFDSIIYHINENEWPYAKAPAKTARLDLIKFFNEM
                     TDLPKEITSETLQSIIEYNSNPKFAKEYMDYFKSVAKIKGLDADTLNYIANPRNLSYK
                     YNIKDIMKIRTELADFAAQNSQKFEGINKYNIQKIFNEIETTEDVNLVKDFINLTSKL
                     NPQISKQNWFKYHFDFNSFSSGKFLDEIKPNTNILKSLEKIVGVESLDEKLLQNLFYK
                     FRNPEDLINLDKFSASFSKLKNNSGQNFQEILINFLKNENSDLSQKMKIKPPLIDALS
                     DFIKTHPIKEESEREAFGQIVKRILNEDSQEKLNFYSDLFKEFEKAPFKFNYLTYSLD
                     WSENHNYNIPSIKSKMKFLEYLEKSEMKSKINENMSENFIGVLLKEIETDENINSFSE
                     IIKQVSENKQIHEFVWYKILSELHNSEPKDLLELFNIRLNFYKMIQAKEPNNLWQIIS
                     DIHNKKDYQECCEILSNIEKHIHSKSADNYLKNIFNTIYCKEELTTFNKLISIEKIKN
                     DSQIFDKIWNKRDILAGKKNLFDIDYCVSLKNSLKSLKQELDAENILKLEQAGISIDS
                     RLRSIEQFLRKAITPIEVPVESMRLFGKNFLANYPQTENILKTFDFTSFGKEGLPLKY
                     SRKSFMADLSQLLKKESVDEKLKIFEKMEIQPKFDKAGNIFAYEGVPNLKNFTAENDA
                     QTKLIDLTRKFMLENEVVSQNPELNKSMNSIIKAFPEYLNFIGKQQQPTHAYSTDIHI
                     MKVLQEAMNHPDYSKLSDLDKTVMKFSILFHDIAKIEGTIDKQHQNVSALYTNNLLEK
                     INMPKEIKNRIVEIVKNHHWSEEFNTGSASAENIATRFRNPQDYLISKIFAQADLKGV
                     NKDFYDIYSSALSPERLAPIEKALNDIYKTGNVIITSRVVTPSKIPQILHQGKTYKVI
                     NMSNLADNQDMAQFGFVPGVKKENIRFLTHFIPDNAMAESAQKIDILSDVSRPGVLST
                     SVLSPQKNKTYNYRKYGFVLESENSNIANAFEMNQGSGVKKNFDNFVQLTTNKNSYRT
                     FLRDNVIKELSLKYPNLDEAEYGEIYKQIIAKRHFRQISDVNTEKRTIKGEDLKQALS
                     NAQDKLFNPSPTEHNEVVVYNPKISGMIFKENAIEDVPQELLDFAHEKNLPIVLFGRD
                     "
     gene            24904..25221
                     /gene="rpsJ"
                     /locus_tag="PKI94_03055"
     CDS             24904..25221
                     /gene="rpsJ"
                     /locus_tag="PKI94_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNW25748.1"
                     /translation="MATATRQRIRVCLKAYDHKLIDVSADKIVETAKRTDAKVAGPIP
                     LPTKRRIYCVLRSPHVDKKSREHFEMRTHKRIIDIYDPTQQTTEELSRLDLPAGVDIE
                     VKL"
     gene            25404..26039
                     /gene="rplC"
                     /locus_tag="PKI94_03060"
     CDS             25404..26039
                     /gene="rplC"
                     /locus_tag="PKI94_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNW25749.1"
                     /translation="MTLGLIGKKLGMTTVFDEQGLAIPVTVIKVDDIVVTQIKTLKKD
                     GYNAIQVGTVEAKEKHLTKAQIGHFAKNKLSNFRYLQEFRIDNPEAYEVGQKIELSVL
                     ENAKKVDVTGKSIGKGFQGTVKRWNFSRGPMAHGSKNHREPGSIGAGTTPGRVIKGKN
                     MAGNMGNERVTVTKLQLVKIDSENSLILIKGSVPGPEGKLVTIKPSRTKWN"
     gene            26140..26772
                     /gene="rplD"
                     /locus_tag="PKI94_03065"
     CDS             26140..26772
                     /gene="rplD"
                     /locus_tag="PKI94_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNW25750.1"
                     /translation="MSKELKILSTNGKELGAMALNENVFGVEPNLHVMHLALRRQLNN
                     ARQGSACAKTRAEVAGGGRKPWKQKGTGRARAGSIRSPLFAGGGVIFGPKPRDYGFAI
                     PQKARKLALKSALSAKASNLVIVKDFSEIKEPKTKLMTKALSSLKVEGKILIIADTKA
                     AENQYLELAARNIPSVRMILPSNLNVKDLLEADYVVITEAAVNEITERLQ"
     gene            26772..27065
                     /gene="rplW"
                     /locus_tag="PKI94_03070"
     CDS             26772..27065
                     /gene="rplW"
                     /locus_tag="PKI94_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNW25751.1"
                     /translation="MSTENLYDIIKKPLITEKSMAATAQGQYSFEVKKDATKIEIAKA
                     VELAFPGRKVKKVRTVYMPSHSKRMGIKAGQTQSGKKAIVTIEGDPIEELIGA"
     gene            27206..28033
                     /gene="rplB"
                     /locus_tag="PKI94_03075"
     CDS             27206..28033
                     /gene="rplB"
                     /locus_tag="PKI94_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNW25752.1"
                     /translation="MAIRTINPTSPGQRGMTRSDYQEITTSTPEKSLLKKIKKTSGRN
                     NTGRITCRHKGGGHKKAYRVIDFKRDKFGIPATVKTIEYDPNRNVRISLVFYADGEKR
                     YILTPEGLNLGDVILSGPEAQVQTGNALPLELIPLGTIIHNIEMEPGRGGVMVRTAGA
                     GAQLMAKEGNYVTIKLPSSEMRMVRKECLATIGVLGNSEYKNLSVGKAGRTRHLGIKP
                     TVRGVTMNPCDHPHGGGEGKTGPGGHPKTPWGKPALGAKTRKVKKASDKLIVRRRKK"
     gene            28132..28410
                     /gene="rpsS"
                     /locus_tag="PKI94_03080"
     CDS             28132..28410
                     /gene="rpsS"
                     /locus_tag="PKI94_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNW25753.1"
                     /translation="MARSLKKGPYVEESLQKKVSKMNETSEKKVIKTWSRASMIIPDM
                     LGHTIAVHNGKTHVPVYVTEQMIGHKLGEFAPTRMFRGHSGSDKAKRD"
     gene            28433..28762
                     /gene="rplV"
                     /locus_tag="PKI94_03085"
     CDS             28433..28762
                     /gene="rplV"
                     /locus_tag="PKI94_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNW25754.1"
                     /translation="MEAKSKQTDIKMTARKLRRIINEVRGKSVIEAQTMLRFMPYFAA
                     RLVEKNLRAAVANAKEKFGVAPEALKISEIFADESVTYKRGKPRAQGRIYRRLKRTSH
                     LTLKVSD"
     gene            28872..29603
                     /gene="rpsC"
                     /locus_tag="PKI94_03090"
     CDS             28872..29603
                     /gene="rpsC"
                     /locus_tag="PKI94_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNW25755.1"
                     /translation="MGQKTHPTGFRVGIIQPWVSTWFAKSKDYAEFIAEDAKIRKFIK
                     KKLYTAGVSRILIARKAQSTTVTVVTAKPGIVVGRGGQGIDDLKNEVSKYLNKPVIIN
                     VVEVARIDADAQLVSEAIALQLEKRIAFRRAMKQAMQRTMRAGAQGIKVMVSGRLGGA
                     EIARSEWAKEGRIPLQTLRADVDYGFAEADTIMGKIGVKVWVFKGNLMPGEKADLNIK
                     AVKKAGNGEEKSMGRRPKRVKTSEE"
     gene            29689..30099
                     /gene="rplP"
                     /locus_tag="PKI94_03095"
     CDS             29689..30099
                     /gene="rplP"
                     /locus_tag="PKI94_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNW25756.1"
                     /translation="MLMPKKTKYRKMMKGRMKGTETRGVNFEFGQYALQAVEPGWITS
                     RQIEAARRAMTRKIKRGGNVWIKIFPDKPITAKPAETRMGSGKGNLEYWVAVVKPRRI
                     LFELDYFDRAEAVEALQLAAQKLPIKVKIIEKAQ"
     gene            30126..30311
                     /gene="rpmC"
                     /locus_tag="PKI94_03100"
     CDS             30126..30311
                     /gene="rpmC"
                     /locus_tag="PKI94_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNW25757.1"
                     /translation="MKLTELREKTIEELNGVVTESKKQLFDLRIQKATHKLENTALID
                     KTKRLIAQAKTILKEKS"
     gene            30338..30583
                     /gene="rpsQ"
                     /locus_tag="PKI94_03105"
     CDS             30338..30583
                     /gene="rpsQ"
                     /locus_tag="PKI94_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNW25758.1"
                     /translation="MPKKEKQGVVISNKMDKTVVVKVASYNPHPKYKKIMESNKHYNA
                     HDEKGLCGQGDIVKIIEAKPVSKTKRWAVLEVVEKAK"
     gene            30689..31057
                     /gene="rplN"
                     /locus_tag="PKI94_03110"
     CDS             30689..31057
                     /gene="rplN"
                     /locus_tag="PKI94_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNW25759.1"
                     /translation="MIQQESRLAVADNTGAKELLCIRVMGSSNKRFAAVGDVIVATVK
                     DAAPNMTVKKSEVVRAVVVRTKADIKRNDGSTIRFDENAAVIINKDGNPRGTRVFGPV
                     ARELRDKNFMKIISLAPEVI"
     gene            31176..31490
                     /gene="rplX"
                     /locus_tag="PKI94_03115"
     CDS             31176..31490
                     /gene="rplX"
                     /locus_tag="PKI94_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNW25760.1"
                     /translation="MQVKTGDRVMVIAGKDKGKIGNVKKVNPSKSNLVVEGANLVTKA
                     QKPNPMAGIQGGLIKLEAPLDASNVMVVCPSCEKPTRIKHDIVKDKKIRVCKKCGEQL
                     DV"
     gene            31589..32140
                     /gene="rplE"
                     /locus_tag="PKI94_03120"
     CDS             31589..32140
                     /gene="rplE"
                     /locus_tag="PKI94_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNW25761.1"
                     /translation="MAKTKSLKETYQKEVKKALKEKFGYKNDLQVPRLNKIVLNMGVG
                     EASHNSKLAEVIEAQLTKIAGQKSVVTKAKKSIASYKLREGMPVGAMVTLRGERMYDF
                     LQKLICVVLPRIRDFRGVSSKSFDGRGNYTMGLKEQALFPEIKYEEVDLVKGMNVSII
                     TTANTDDEARALLTELGMPFKKG"
     gene            32250..32456
                     /locus_tag="PKI94_03125"
     CDS             32250..32456
                     /locus_tag="PKI94_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNW25762.1"
                     /translation="MAKKAMMNKEARRIELAAKGKYPKVRLHNRCSICGRPHGFHRDF
                     GLCRIHLREMAHRGLLPGVVKASW"
     gene            32570..32965
                     /gene="rpsH"
                     /locus_tag="PKI94_03130"
     CDS             32570..32965
                     /gene="rpsH"
                     /locus_tag="PKI94_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNW25763.1"
                     /translation="MNTDPIADMLTRIRNANLVSHETVEMPSSKLKVELAKLLKEEGY
                     ITDYSTRQSGKFTFLTVVLKYDEKRKPVISNLRRISKPGLRSYCKAKNLPQVLGGLGI
                     AIVSTSKGLMTDRKARKDNMGGEILCYVW"
     gene            33132..33671
                     /gene="rplF"
                     /locus_tag="PKI94_03135"
     CDS             33132..33671
                     /gene="rplF"
                     /locus_tag="PKI94_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNW25764.1"
                     /translation="MSRIGKLPIEIPQGVEVTIEGQTVTAKGPLGTETVDVREEILIK
                     KEDNKVILTRRDEERKTRAIHGLSRTLVANAINGVKTGFEKSLELQGVGYRAAMQGTT
                     LNLQLGYSHPINIVPPQGITISVEANTKITIKGPNKQTVGDVAATIRAKRPPEVYKGK
                     GVRYEGEHVRRKAGKAGKK"
     gene            33827..34195
                     /gene="rplR"
                     /locus_tag="PKI94_03140"
     CDS             33827..34195
                     /gene="rplR"
                     /locus_tag="PKI94_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNW25765.1"
                     /translation="MIKTKTRKEQAQKRHQRVRIKLSGTTEAPRLAVYRSTKHIYAQI
                     IDDIKGVTVASASSVDKDLKEKLNHGGNIDSAKAVGEAIAKKALKKGVKEVVFDRGGF
                     LYHGRVAALADAAREAGLVF"
     gene            complement(34289..34444)
                     /locus_tag="PKI94_03145"
     CDS             complement(34289..34444)
                     /locus_tag="PKI94_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25766.1"
                     /translation="MLRKIETPTNNIYGGVEFILRGAKKRRSMALATAKVLNSEIGIQ
                     TRLVAVK"
     gene            34908..34981
                     /locus_tag="PKI94_03150"
     tRNA            34908..34981
                     /locus_tag="PKI94_03150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34942..34944,aa:Arg,seq:acg)
     gene            complement(35242..35469)
                     /locus_tag="PKI94_03155"
     CDS             complement(35242..35469)
                     /locus_tag="PKI94_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW25767.1"
                     /translation="MQHYKNISSYIKQRRLETGISLNEFAFNNDLEPATLSRYENGKR
                     TISLMNMVKIANGFNQTLAEFLADFEKKYFK"
     gene            35548..36360
                     /locus_tag="PKI94_03160"
     CDS             35548..36360
                     /locus_tag="PKI94_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25768.1"
                     /translation="MKKVKIYTWADKTPEFIYRQLETINKFVKDEDFEFIVFNNTPIT
                     AYDRRYRIKFICQKLGIKCLDVRFRSFISGSAQICGWGIHWAFHRFFRWEKDTIHVVL
                     DSDMFFIKDFCFNEYLGDNDICAIHQRRENIEYPWNGIMIFGGNLPDKTSLDFRLGTI
                     NGIRTDVGGKIYYWLKKNPDLKIKWMGHTGHLDISKSIILPESLRHEYKPEYCFQIIE
                     NSILHYRAGSNWDKCHFSFVDEKRAYFDKLLNALVYNGEKIEVSSEFNIYMQ"
     gene            complement(36432..37028)
                     /locus_tag="PKI94_03165"
     CDS             complement(36432..37028)
                     /locus_tag="PKI94_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25769.1"
                     /translation="MLRVKSFILCVFVMLLLSFVGSKAVAADYSANTSDALINRAITL
                     MEDTYMTSSIKVLQGDNSTGQAIEVKFANLSAISIDYMSANAVTVVNEDGKMVIYISK
                     SFKTAPAEAIACLIRHETTHNDERCSIEEEVRAWTNEAAAWVYFKSRNPGLARTEASS
                     SKLVVRLDYLSNLYKAGGNSLIRKEVLANGMYSQLAMR"
BASE COUNT        12725 a         6496 c         6378 g        11589 t
ORIGIN      
        1 aacctgtttt tgggcgaaaa attgtccgaa tatcacacag gattcagggg ctttacaaaa
       61 gaagttttac aaaaattacc gcttgaagaa tgcagtgacg attttatttt tgacaaccaa
      121 agccttgctt tgctttttta ttacagatat aaaatcggtg agctttcatg cccgaccaaa
      181 tactttaaag aagcatcttc aattaatttt ttccgctcgt gcaagtacgg cttgggagtt
      241 ttagccgtaa gccttcaata tagacttgca aaaatgggat taaaatcaaa aatatttaaa
      301 acaactgcaa aaaaattaga tttagatgca aaactggaat atttttacaa aaattaattt
      361 atatagcaat aatttttatt catatgtttt aaagcagtag ttacaaaaca tatggaataa
      421 aaatgtctta tattggtcgt gtttcattta aaatagctaa ttattttagc actataacct
      481 caaaaacccc tgtcacaaaa aggacttttc aagcacaaag agatactttt tgcagcacca
      541 attcccaaat tgaagatgat tttgagctta aaataaccga tgaccctaat ttattagaaa
      601 aaaaaataaa gcaaaaaata ctttcggcaa atattaacgg ggaaacatta accggctggg
      661 atgcagaaat aagaagtgaa gcaacagcag caatttataa aaaaaatttt tttgacctgc
      721 tttctcaaaa agctcaaacg gttaaagatg aaaaaataat tactcatatt aaccaaattt
      781 ttaatgaaat attctgtaaa tcaaaaatta cttcagaaga gcatatccaa aatttttggt
      841 attttagaga tttgcttgat gcatacaata tgagtgaggg cttatattca aaagtgggga
      901 aaacagacga atattacaaa atttatacaa aaaatgaaga tgaagattat cagaaatgtt
      961 taattaattt aattttttca gataaaaatc ttttatataa aattttggaa gaaaatccca
     1021 ataaaaataa cagcttagca aaatggcttg ttaattttca aaatgacagc cgatttgaga
     1081 gtttaaaata ttatattaaa aattattctc tttcacaaaa agatatgaca aactacatgt
     1141 atgaaaaata ctatttatct caaattccag aaaatacaaa aaaattatgt gaaaaaattt
     1201 cagctgaatt tgggataaaa atgtttttac acaataatta cgacaaggaa aatttaaata
     1261 ttatatatga tgagctatca aattggaaaa aagcaagcca aggtaaagct ttattcccgc
     1321 atgttataga tttaagcact atagatcccg attatataaa agaaaaaagt ccgtctgctg
     1381 cttatttcag tactgcatat aaatttatct gtgtgaacgg caatagacct ttaaatatgc
     1441 aaaaggggag tttaaggcac gaaatgactc atttaaatga cttgaaaccg aatgatgaca
     1501 gttattttga aaatcttctg gaaaatagaa ataaatttca gcaagaattt gctaatgccg
     1561 gaatagacca agaatatatt gactatgcat ttaaaaaaga tgcaagcgag ttcaaagcag
     1621 ttgcaaccga aggcgattat tcaaaatatt ccgatgaatt taaggactta cttgtgaatc
     1681 tcgggcttcc taaatgggtt tttaatatga cacctgtagg taaaatggaa aaataatttt
     1741 caccctcaaa tatattgtaa attaatcctt tttagtgtag atttatatca aggttgttac
     1801 ataaaaagga gagctcacaa tgagtgaaag aaaacttgtc ggaacaaatg aaatgttcaa
     1861 aaaagccctt gaaggcaaat acgcaatcgg cgcatacaat gttaacaata tggaaattct
     1921 gcaggcaata gcagaagctg cagaagaaac ccgctcacct attattttgc aggtttcagc
     1981 gggcgcaaga aaatatgcca accaaactta cctgataaaa ctagttgaag ccgcacttgc
     2041 tacaacaact gtacctattg cacttcacct tgaccacggt gctgattttg aaatctgtaa
     2101 atcctgtatc gacggcggat tttcttccgt tatgattgac ggatcaagat tcccgctaga
     2161 agaaaatatc gcgcttacaa aacaggttgt tgaatacgct catgccagag gggtttcggt
     2221 tgaagccgaa ctcggcaaac ttgcaggtgt tgaagacgat gtaaacgttt ctgcagcaga
     2281 cgccaaatac acaaacgttc aagaagcagt taaatttgtt acagaaacag gctgcgattc
     2341 cttggcaata gcgatcggaa caagccacgg tgcttacaaa tttgcaggtg aagcaagatt
     2401 ggattttgaa agacttgcac aaattaaagc cgcattaaaa gaagcaggac acggagatta
     2461 tccgattgta ttgcacgggt cttcttcggt gccaaaatat ctggtcgaaa aatgtaacca
     2521 attcggcgga caattaccta acgctcaggg cgttcctgaa gaaatgttat catttgcttc
     2581 taaaaacggt gttgctaaaa ttaatatcga cacggattta agacttgcaa tgactgcaac
     2641 aataagagaa ttctttgtga cagagccggc taaattcgac cccagagaat atatggggcc
     2701 gggaagagct gctgttaaag aactcgttaa acacaaaatg caagttcttg gaacagctgg
     2761 acacgcggaa taatatgtca ttgcgagggc tttagccaga agcaatccaa ataaaataaa
     2821 agggcggata ttctttcaga atatccgccc ttttaatatt ttctaaaccc ttaaactttt
     2881 ctcttacgag cagcttcgga tttgcgtttt cttttcacgc tgggtttttc gtaacgttcg
     2941 cgtcttttaa tttcagacaa aattcccgct ttttggattt ttttcttgaa acgacgaagt
     3001 gcgctttcga tactttcgtt atcccctact tttatttccg gcattgcttt ttcaccacct
     3061 taaggtctta tttgtgtaat caatattgct attttaaaca aaacaaatta aaaagtaaag
     3121 gggaagcggt gagggggtaa atgggtgaga agtgagaaga aaacttataa actcataatc
     3181 ttataacctc ataaactttt caactattcg actattcgac ttttcaacta aaaagctaca
     3241 tttgtgaaag ttttatcaat tcgtcaacaa tttcttcata agaaaatttt tcgcccactt
     3301 cctgttgcaa aaaggaatta attttatcca tcatatcgat tgctttgtcg cattttggat
     3361 cagagccttt aacgtaagca cctatattaa ttaaatcttc attcttttta tagactgcaa
     3421 gaagatttct caaattacct gccgcctgct ggtgttcttt tgaagtaaca tcgggcataa
     3481 ccctgcttat tgattccaaa acgtcaactg caggataatg gtttctatgg gcaaggtcgc
     3541 gcgaaagcag aatgtgaccg tctaaaatac ttcttgcggt atctgaaata ggctcgttaa
     3601 agtcatcgcc ttcaaccaaa acagtataaa gccctgtcat tgtacctttt tcgttacttc
     3661 ctgcgcgctc caaaagtttt ggcatttgag caaaaacaga aggagtataa cctcttgtag
     3721 ccggcggttc gccgattgca agcccgactt ctctttgagc cattgaaata cgggttatac
     3781 tgtctaacat aaacaaaacg tcacgacctg tgtccctaaa atattctgca atagcggttg
     3841 caacctgtgc ggcttttatt ttaaccagag aaggctgttc ggaggtggct gcaactacaa
     3901 tcgttctttt catgccctcc actccgaggg ttttttctat aaactctctg acttctctgc
     3961 cgcgctctcc cacaagcgca attacgttca tatcggcgga agtatttttt gccatcattg
     4021 caagtgccgt gcttttgccc acgcctgagc ctgcaaaaat ccccattctt tgccctttcc
     4081 cgacagtgga aaacccgtca acagatttta ttcccaaaga aagtgcttcg gttattcttc
     4141 ttcttttaag cggatttatt ccctccgaac ttgtagaata aagagtttgg gaatttattt
     4201 cgccaagatt gtcaagcggt ctgcccagtc cgtctaaaac tcttcccaaa agctgttcgc
     4261 ccactttgat tttcatagca ccgccggtgt ttactacaat tgctcccggc tgcaagcctt
     4321 ccatagagcc caaaggcatc aacagaattt ttgactggcg aaagcccacg acttctgccc
     4381 atacaggagg ctcattgtaa tttctgtaaa tgtaacataa atcgccaatg ctcgattgcg
     4441 gaccgtcgga ttcgataaca agcccgataa tttcggtaac cttaccgatt tcacgcatgg
     4501 tgcaggtttg ctgtattata ttagaatatt tatcaaaatt tatcattttt taccttgtca
     4561 tgctgaactc gtttcagcat cttgccaaca tagtatgctt gatagatcct gaaacaagtt
     4621 caggatgacg cttaaattaa attttcattt tcagctgata atgtttcact tatttcaaaa
     4681 ttaacctgcg tcattaattt ttcagcaatt tcgttaatct gcgatttcac cctgcaatcc
     4741 acccttgaaa gtggtgattc tacaattgtt ccgtcagggg aaacgctgtt gtcttcaact
     4801 atttttattg aagaaagctg aggaatttgc tctttaattt catccgaaac acctcgaatt
     4861 tgctctgcca taacaggatt tacgataatt tttatgtctt ctttatcttt taaagactga
     4921 atggcacctt gggttatttc ttttaagata tttttatcca aatcaaattg tttcatacaa
     4981 attttttcgg atatttccaa aacaagctgt attatatccg aatgtgccga tttgactatg
     5041 ttttttttga tgtcaaatgt actttgggca aaattatcaa ccgcaagaat cttttgagaa
     5101 agttcttctt taattttttc agttccttca atacgacctg cttcaaagcc ttcttgaaaa
     5161 gcttgttgat gaattgcctc aacttcgttt agtgcctgaa ctttggcatt ttctataatt
     5221 tcatttgcct gtctttgaga atcttctatt atttgtcttg ccttagtttc accttgcaag
     5281 acaatttctt gttcctttga cagagcttct ttgatttttg tactgcttaa actctgctca
     5341 atcggaactt caaaagaacc gccgagtttt atattgtcac tttttatcct agtcaattaa
     5401 ttcgtcctca acgttttcac gtgcaataat aatctcacct gcaacttcaa gagttctgat
     5461 aacaccgaca atttttgtct gaatttcttg aacttctctt gctctgacag ggcccatata
     5521 ttccatttca tcacgcagca tctgttgtgc acgctctgac atatttttga atactttttc
     5581 ttttacatct tcttttgtac ctttcaaaga cattgcaagg tcttttgtat ccacttcgcg
     5641 aagaattctt tgaattgctc tgtcttcgag atttactata tcttcgaata cgaacatcaa
     5701 ttctcttact tcttctgcaa gttgcgggct ttgaacttcc aaaagttcta ttacgtttct
     5761 ttctgttgcc ctgtctgctc ggtttaagat gtttgccaaa gcttctacgc cgcctgcttt
     5821 tgagaagtcc tgaaccacaa cggatgaaaa cttgctttca acaatttttt caatttcaga
     5881 taaaatttca gggtttgtgg tgtccatatc cgctattttc attgcaacct gcgcctgaac
     5941 ctcaggggga agatagttta aaacctgcgc agaatgttcg ggctttaagt aagccatgat
     6001 aagtgctatt aactgaggat tttcgttttg aaatgaagtt gcaagctgtc ctgggtctgc
     6061 ttcattgaaa aattgaaacg gatttgaatt catcaaaaca accaatctgt ctaaaatctt
     6121 tttagcagca tcccctccat aggctttctc caagagctgt tttgcataat ccataccacc
     6181 tgcggaaata taattgcttg cctgaaatat cgctttaaac tcttccaaaa tccctgtcag
     6241 aatttctgtg ggaaccttat ttaaacttgc aatatcaagg gttatctgtt caagcaggtc
     6301 atcgtctgtt atatttttca aaacctcaga agcggttgcc ggtcccagca cgattaaaag
     6361 tgctgcgact ttttgagttg gtgtcattga ttgtatctgt aaatcaggta acatttttaa
     6421 tccttaataa atgaggtgag taatcttgct gcctctgacg gatcggcaag aattgtatcg
     6481 ttaagttcca ttcgaactct ttcaagctct gggtctaagt gggcttcaat ttgaggcagt
     6541 gcctcatttt ctattaaatc ggtcggctct tcttttatag tcggaagctg tgcttcaaac
     6601 atttttgaat tgtaagtttc gtttgcaaca tttacgccgc caccgcctgc gccgattcct
     6661 tttgtaagac ttttaacaac tattagagca cctgctccta aaatcaggac aatcaataaa
     6721 ggtgctattt tagttgtcca aaattctata ttattttcct gtgccaaatc tttttccatt
     6781 tggtcttgag cggctttgtc ttcggctatc gattcaaact gcatgcttga aaccgtaatt
     6841 acatcgcctc tgttgtaatc agctccgctt gcagaaagta caaggttttg aatttcttct
     6901 ttttcttttg gcgttaaaat tttattcacc gcaacggcta tggtcattct tttaaccgtt
     6961 ccgggtgcat agattacctg cttaatttct tttgaaacgc tataatttgt tgaatttttt
     7021 tcttttgaat agttaagatt tttgttcccg cctgcagcag caggagcagg accctgacca
     7081 ggatttgcat aattttcggt ttcattttgg cttgtggtta aaacgccttc gcctttgtcg
     7141 tttagaggaa tataactttc tatggtcgat tttgcactgt caaaatttat atccgcgcta
     7201 acctgcactg tggcatttcc tttacccatt attttttcaa gcactgtaat tactttttcg
     7261 gaagtttgtt tttcaaaatt ggacttgaaa gtttccatat cgtttgaatt cttgccgttt
     7321 tcgtctccca aattgtttcc gttctggtcg gttatgaaaa ctctgtcagg agttaatcta
     7381 ggaacagcat aggcaaccaa attcttaatt gctttaacct gtgagcctct taacttataa
     7441 cccggattaa gaattagcat aacagatgcg gtaggttctt cgtcttcatc ttcaaaaatg
     7501 gaacgttcgg gatctgctat ctgaattctg acgcttttta ttccgttcat attttctatt
     7561 gaacgggtta attccccctg aaaaattctt tgttttgtaa gcttattttt aaaatcggtt
     7621 gaacctaaag gaagctcgtc caaaagttca aaaccggggg atgaattttt aattaaatca
     7681 ttttctgcaa caaaaactct aagttcatct tttttgtctg cctgaacaag tatggttttt
     7741 ctgtcatctg caattttata tgcataaccg tttttcttta aattttctac gacacttacc
     7801 gcatcaggct cggacaaatc ggtatataaa accgcccaat taggctccat tgatttaatt
     7861 ataaagtaag tagccacaac aaaagtaaca atcgtaaggg taagcatacc aaatttttgg
     7921 gcaatatcaa gcccgttcca caattttctt atatcatcta tgaccaattt aaagtaatta
     7981 ttcaaatcct tccccttttc ggtgagcggt gagcagtgag aggaaaatcc ttatttactc
     8041 attcacctat tcacttattc acttctttac accactataa cctctttttt tgttcatttc
     8101 aattatgtaa acatcctgtt accattgact cttactaaac cttaacgtta ttaatttcgt
     8161 tgtatgcagt tataatttta tttctgagct gtattgccat ttgcatgctc aaattggctt
     8221 tctctgcggc tatcataacg tcatgtacac ttacattttc acccatagcc attgcttctt
     8281 gcgcgcgttc gaaagtttta gtactttgat cgacagaatt taatccgccg ccaagtgcat
     8341 ttccaaaact gtttaagaaa tttgcagcag aattttgctt aatcctggaa cttgaaagta
     8401 cattttcaaa atcagaatta actgcgtctt gacttttttg tacaacattt ttttgcctga
     8461 gcgtgtcttt ttgcctgtct aatattgttt caaaacttaa atcggaatta acctcaaaag
     8521 aagcacttcc cttaaggtaa ttattatatt ctgctaccgg tgaaaaattc attccctcaa
     8581 taggccctac gaaattcatt ttatttctcc tttttgtgtt tttcaacacc ttttgtattt
     8641 aataaatagt gaattaaaaa tctatttact atttattatt ttcttatcat atcttcattg
     8701 ccgcagaaat catattttta acgttttctg ctgctaccga gtttgcctca taagcccttg
     8761 aagcttctac cattccaacc atttcttcta caacatttat atttggaagc gtaacgtatc
     8821 cgtctgcatt tgcatcaggg tgcgaaggat cataaaccgt tttgacagca tttttccctt
     8881 caacaattcc gtctattgca actccgcttg acatttgacc gtaattcatt gaaacgccgc
     8941 tgtttatttt aagcgttcct gttgcaggat tgaacgttgc cgaagtcgag ttgtttgaaa
     9001 aattcgaagc cccttgcgac attttttcat cataaatcgc cttaaaactt acctgctttt
     9061 ttatgtaagg accgacagac ccgtcagggt ttcttgttgt gtttatgttt gcaatgttgc
     9121 ttgaaatggt gtccattcta accctttggg cgttcattcc tgaagctgct acatcaaata
     9181 catcaaaact catttttgct cctttttttt aattaaatgg tgaataaatt tctatttact
     9241 atttaccatt taccatttac tgttttattg tcctttaatt acatccaata catttttcaa
     9301 agccctttgt tcaaggtttg aaagaatagt atattgagta ccgacctttg ccatatccat
     9361 catttctttt tcaataacaa cattatttcc gtcttgtgcg gctccggaca ttgtatcatc
     9421 agtgatttga ggctcaaatc ttgaataaat atcgctttgc aaataatttg ccttttgtat
     9481 agttaaattt tgcggctgcc cgaaacttgt aacttcatca attgaagaag gattgtactt
     9541 aatactgttt tgttctttta tataagtcct taagtcgtct ttttcaacca tttctttcag
     9601 ttgattttca aagcttactt ccttgcggac atagccgggc gtcattatgt ttgcagtgtt
     9661 tgcggtaatg gctttttgac gctccattaa tccgtcaagt gcgaattttg ttatatctat
     9721 tgctcttgtt gatattaaat ccatttttta ccccttgttt aaaacaattt caaaatctgt
     9781 ggaaacttcg tctgatttta tcaatctcaa aaaacctctt tgttcttcga ttgatttttt
     9841 gcaaattcca agccccaaac ctgagccggt ttttttagtc gtaaagcctg tttcaaaaat
     9901 tttgttttgc aaatcttttt ttattggctc tccggtattt gaaacgagaa tttcaatttt
     9961 atcgtttttg tttctactct cgattgttat tttgcctttt ttgtcaatac tttctattgc
    10021 atttttaatc atattaatca aaacggaaca gagtttgttt tcatcggcaa gaataaggat
    10081 atcttcacct atatttaact ttatttcgat atctttttca tgcgcgtaaa tttttccaag
    10141 ttcaacgaca ttttgtataa gcggttttaa ttcgacactt ttgagggaag cttctttaaa
    10201 agattttagc tccaaaaggg aattatttgc caatttaacc gatttttgaa tactgtttag
    10261 cgcattttct attgagttta atatgctttc ttctgtactt attttattaa gatgtttttg
    10321 aataatattt gaatacagat cgcatatcga aagctgattt cttacttcat gtgctacaga
    10381 acttgaattt tttaaaacta aattcatctt catcatatct tcggaactgt tttgaatttc
    10441 ttctttttca atttctgata tcaaaacttc ctgattggtt tcgttaagat tttcgaccat
    10501 ttttctgata gaactgttta aatggtcgtt atcccttctg tcagggtgcc atttgtcctg
    10561 ataaagattt aagtcctttg ttgaatttga atttatcata ttaaaagcat ccgataaatt
    10621 tttggaatta tacaagatat aaatttgcgc ataaccttca atttcgccct gctcatagtc
    10681 aaaaaccaag accgcgccgt acctttgggc aagaacatag ataaattcgg ttttatccca
    10741 aattttttct tttaaaacgt aatcaaattt ttcgctcaaa ggtttgtcgg aaaaatcgta
    10801 aacctctgca cttgaatatt caagcctttt taaaatgctg ttgaaatctt ttttggattt
    10861 atcttcaagc ctgcacataa taacgctttc aacgcttgcg tccaaaatcg gttcaactat
    10921 tgcacgaaac agacctttta tggtctgctc gttgcaaggc ttgttgtctt gtatatatat
    10981 caaatctttt aatttgtttt tgtgcatttt aattttccta taagtgaata ggtgaatagg
    11041 tgcaattata ataaatttaa cttttcagcc tttcaccctt tcagccctaa accgttgtca
    11101 actgcatttt tcttttgttt gtaaaaccgt tgttgattgc ataaagcacc gcttgggtcc
    11161 tgtcatttac ctgaagtttt tggaaaatgt tgttcacgtg agttttaaca gttgtttccg
    11221 agataaacaa ttttcctgcc actcctttat aggtaattcc ctgagtaaga aggtcgagaa
    11281 cttcttcttc tctttgggta agataagaaa ggagattttc ttcatctgag atgattgttg
    11341 tttcttcttt gaaagagcgt tggaagtact cgaaaaatct tgaagaaagt gcggcaggaa
    11401 gataaacttt acctgcagca acttcttcaa tagcataaat aagttgagcg gacgccatag
    11461 tttttaaaac gtacccctta gctcccagct tcatggcgcg gaaaattaaa tccgaatcat
    11521 catacccgct caatgcaacg caacgcaccc ctaagttcag cttctctatc tcctgaattg
    11581 cctgaagacc atctttttca ggcatgttga tatccattaa aacaatgtcc gggcggtgtt
    11641 ttttcacaag cgaaatccct atattcgcgc ttgtagaagt agcaaccact tcaaattggc
    11701 tttctaaatt aataatctct ctgatacccg ccaaatgttc ataattgtcg tcaattagca
    11761 aaactctggc ttttttctta aattccaccg ctcttctcat caatctcctc cttatttaag
    11821 taaatataat ttactattcc ctaacttaat ctactccgta catattacct ctttttagag
    11881 gaggttatca tcaacataaa ggtcgatttt ataagagttt tgggtagata agtcgatata
    11941 gaccacatgg tctatgaata ggtgagaagt gagcagtgag cggtgcgaag ggaatttatt
    12001 aaaattaaaa atcaaattat aaaaattttt ataattaatt taaaagtact agtctataaa
    12061 ggtgaatagt aaattgtaaa tagtaaatag aaaggaggat acggaaaaat tcgaaaaata
    12121 attctgaatt tattaaaaag aggcgggctt gtttaatgac aagcccgcct ctttttaatc
    12181 acttattttt taaatttgag catgcgtcaa aatgtttccg cctttgtcca aaagaacttt
    12241 ttctatattc tcatttggat aataatactc ggcgcggtta aattttccgt cttttcttat
    12301 taatattttt tcaataagtc cgttgcttgc ttcataatat ctttttattc cgggcaacaa
    12361 ttcttgcata ttaatacttt cttcggttaa acaatttctt acagattcgc ttgtaagctt
    12421 atttgccaaa gcctcttgag ccactttcaa tgattcaatt ttttgcggtg ctaaatcttg
    12481 tttcgtcaac aaaattaaat cctcgccgct aagattttta aaatccatta aagattgagc
    12541 tctgctatat tctacatcat ccaattttgc aaaagtaatt atctgttgcg cactaaatct
    12601 tttttcttta ccggctttaa cagcatctgc caattcttca agccttaaag actttgaagg
    12661 ttcaaccttt gccatttctt tcaaatcttc gatgctaaaa cgatattttt gaccgttttg
    12721 ccctttttct tttataagtt tatttaaaac cccgacattt tccattctaa gcctgtcata
    12781 aatttcaaag ctgtcagtat ctgaaaattt tcttttgccc aaagcctttt ttgcaacacc
    12841 tatttctttt gaaaacccct tgagtccttg ttgaaaaaca tcttgattag ggcttctgac
    12901 caaatccgac tgaacagagc cgaccatttt ccttgttaaa acacttactt ttgtaaaaaa
    12961 tccgttaaaa aaaactttca ttttaaaccc cacttttgaa tataactaat tacttttata
    13021 aatataataa taaacaaaca aaattttttt tgccagtaaa acaaaattta tataaaaatt
    13081 tgtaatgttt tttcatgata aaattattta aaaagaggtg gatatggaaa tattgatttt
    13141 tatagcaggg tttgttttag gtgcagggct tgtatttgcg atttttgcgc tgactcaaag
    13201 aaaagccgag gatgccaaag aagaactaat aagccgcatg cagcttcagt ttgagaattt
    13261 gacaaacaaa atcttcaaag aaagttctca ggaattttcc gaacaaaata aagcaaaatt
    13321 ggaagaattt ttctcaaaat tcaggcaaag gatagaagat tttgaaaaaa gaacagaatt
    13381 taatttcaaa accgaagccg aaaaattaac ccaatttgac tcaaatgtaa aaacttttat
    13441 tgaagcggga aacaaaattt ctcaagacac aacctcactt gcaaccatga tgaaaggcga
    13501 caaccgcagg cagggaagct ggggcgaaat cgtgctcgag agagttttgg aagcttcggg
    13561 tctaagagaa ggtcaagagt atgaacttca aaaaggaatg accgatggcc gccccgatgc
    13621 cactattaac ttacctgagg gcaaaaaagt ttatatcgac gcaaagactt ctcttgcttc
    13681 ttgggacgct tacgtaaatt ccgaagatga aaccgaaaaa gaagaaaatt taaaagagtt
    13741 tttggaatcc acaaaagcgc acataacggg gctttcaaag cgtccttatt ctttatcgga
    13801 cagctctcca gattatgttt taatgtttat tccgattgaa agctgctatt ctctgctttt
    13861 ctgcgaagac agcaagcttt gggacttggc ttggaaaaac aatgtcatgc cggtttcgcc
    13921 ttcgactttg cttgcagcac taaaaataat aaataatttt tataaaatag aaaaacaaaa
    13981 caaaaacgca cttgaaattt caagaatttg cacaaaaatg ctcgataaat ttgccgacat
    14041 gctaaaagat gtgcttgatg caagaaagaa aatatcatca gctttgacaa ggctcaacgg
    14101 gcatgacagc ataataagaa acattgaaaa gcttcaagag ttgggtacaa cgataaacaa
    14161 acaaattccc gaaatcggag atgatgtttt ggaagaaata agttaaatat taatcgatta
    14221 tttattatta ccgattaaat cacaaattct agctatcgtt tttgggaaat attgctgaag
    14281 gtaatgtgcc cttatgccaa aacaagaatt tgactgataa gtatttaaaa tagcatttgt
    14341 ttccgcgatt aattcatgca gattcgattt aggagttcta tcactcgttt ctgtaaaata
    14401 attaagatga ttttgttgta ataatgtact ggcattttta aataacagtt gctcttctga
    14461 gaatattttt aaaagctttt cgtcatttaa aatttcagaa gttacttttg tatgtcctaa
    14521 ttcatgtccg aatgaaaaca agttatctcg gcaactaatg acattggctg ttgggctata
    14581 tccagataaa taatcatcag caacataaaa gaattttgtt ttgtttttaa tcattgttat
    14641 aatgtcttca gcattgattt ttttgaaaaa ggctattgtt tctgcccttt tgttttcaat
    14701 aatgtcgtca atattataac taagcacttc ccctgtatta atgttattta taattactga
    14761 attatcaact atttcagtcg tatattttct tccgttaata tttgtaaatg atttattatc
    14821 gttaatcact tcaaatatat ttgttttatt aagcagagtt tcaccgtttt catcaataat
    14881 tctatatagc tgtttcctac ttcccgaagg ggttttaata tagtaatatt gtgttttgac
    14941 attattgttc cctctaatat tttttttgat tattacattt ccgtttttat cttttaaagc
    15001 tttgcaataa ttaataagtt tattttgttt atccttgtaa tttaagttat aaacgccggc
    15061 tacagaagat ttttgtaaat aaaaactttc aatgatttcc ccgacattgt ttctgaatat
    15121 tattctttca tgtagtattt cttccaagcc gttacataac aaacttatgg tttttgattc
    15181 ggtattattt ctgaaatcat gtagtgtttt aattaatttt ttagagcctt tcttcaagcg
    15241 ttctgtttta atttgccctt cagataaaat ttctccgttt ttattcatta aaataatttt
    15301 agaattatgg tttttgaaca atatttcagt aaaaatatta tttaaaggtg cttcaataat
    15361 tcttatatta atttctttta tttgtccttt aatattttcg caagctaaaa ttaatttttg
    15421 aaatattttt gtataatcaa tatcgtttga cttaaaaaga ttttctatgt tttgttcaga
    15481 caatttttta attaataatt tttttatatg ccttttttct ttttgctttg atattctttc
    15541 taagaatagt gttttcttga aatttaattt tctcttacct ttttgccctg taaggttact
    15601 agaattgtct gaaggcaaaa catgatttgt gttcgcattg cgttcatgac ttgtaaagta
    15661 tggtttaaaa aaattaattc gattaaaaat tattcgcata atttgccttt taaaaaaatc
    15721 ctagcatcta taataaaaac gataaaaaaa ttttttgcta gcactatttt ataaaagaat
    15781 atgtggctat tttatcagga ttttgcttgg gttttagtca actttattaa aaaaatttat
    15841 tgataaagta aatagcaatt aatatttact tcttaatcat agaacgaatt ttcaaatatc
    15901 tgtcgagttc gcctctgagc ttacccaaat cgtcataaat tccgttatac aaaggaattc
    15961 tgttatcaat ttgcttatca ataataaata ttgaaggaaa tccgcttatt gaatagtctt
    16021 cgacaagttt ttggtatttg ggattttcta catttatcat gacaaaattg tatttgtctt
    16081 tgtaaatgtc cgaaatgagc ttgtatttgg gcataaaacg tatacaatag ctgcaccagt
    16141 cagcatagaa aagagcgata aaaggttttt tatctttaac agcctgttca taggttatcc
    16201 cgattgaata atcagagggt tttaaatttt ttttaggcgc aaaagccatt gtaacagcag
    16261 aggtcacaca aatcgcaata attatcccca atatcattaa aatctttttc attttatcct
    16321 caatcggtgg aatagcaatt ccaccctgct gttcttttca gctcactgct cactgctcac
    16381 aatttggaac ctttcggcac aacggtaact ccgccgggag aaacatggaa tcctcttttt
    16441 tcgtcttcct gtttgttaaa tccgattttt gtataaggcg gaatttcaac gtttttgtct
    16501 attatcgcgc gtctgatctc agagtgtctt ccgatagaaa cattttccat caaaatactt
    16561 tctgatactt gcgaaaagct gttaatcctg acttttggag aaagaatgca tcttgaaatg
    16621 cttccccctg agattataca accttctgcg accatggaat ttgttgccat accgaccctg
    16681 tcgccttctt cccaaataaa ttttgcaggc ggataattat aattaaaagt ccttatcggc
    16741 cattcttttg aataaagatt taattcagga caataatcaa gcaaatccat gtttgtctgc
    16801 cagtaagcgt ctatgcttcc tacatctctc caatagcctt tttctgaatc cgacattcca
    16861 gggactttat tttgagcaaa gttataaaca tatatttttt tcttttcttt taaaagcgtc
    16921 ggaataacgt ttttaccgaa atcatgattt gaaacttttg tttttgcatc ctgattgatt
    16981 gcatttaaaa gaatatcttt gttaaaaatg taattgccca tagaagcaag gcacatattg
    17041 ggattatttg gaattgtttt tggttttgat ttgggttttt cgacaaaatt tatcagcttc
    17101 caattttcat ccacttccat aatcccaaat tccgaagctt cactaacagg aattggaata
    17161 gcagaaattg tcaagtcaga gcctgttttt ttgtggaaag caagcatttg ggatacatcc
    17221 attttataaa tgtggtctcc gccgaaaata cagacgtaat cggggttaag gtcgtgaata
    17281 tgatttatgt tttgaaaaac ggcatcagcg gtacctttat accattctcc gccggttctc
    17341 atttgtgcgg gaacaaggtc aacaaattgg ttcaaaaaag gcgacaatgc ccagcctctt
    17401 gtgatgtgct tattaagcga gtcggatttg tactgtgtta aaacttttat cttaaaaaat
    17461 cccgagttga taaaattatt caacacaaag tctataattc tataccttcc gccaaaagga
    17521 acagcgggtt ttgccctgtc tcttgtcaga ggatgaagtc ttttaccttc gcctccggct
    17581 aaaatcatca ccaaaacatc ttcattcgcc ataaacactc ctatttaaat tttgattata
    17641 ctataaaata taaaaaaaac cactatttta caaaagaagc aacccattta actatatcag
    17701 aagacattgc agggttaact tttatcatca acattcccat gccgccgttg gggtaatttt
    17761 taacataaaa agctccttgc gaaaatcttg acagtttttg ctgttcttga agacaaaagc
    17821 tgtctttttg gcttgttata gcaagcacag gcacataacc tatttcagaa agagctatgg
    17881 gaacatataa acctttaaaa gtcattgtag gagaaattaa aacaagtgct tttggcttca
    17941 cagaaagatt ttttgaaacc aaaatcgcag tatttgcgcc tatatctgca cctattatta
    18001 ccaacctgtt taaatctgtt tttttagatt gtttatcaat ctctgaaagc atttgtacaa
    18061 catctgaagg gaatttttga taaactttat ttgtaaaata ctgccaagaa cgctgcttta
    18121 agtttgcatc ataaacgctt tttccatgcc ctcttaaatc cattttaata acagcgtaac
    18181 ccgaatttac aagcatatct gctaaattac cccagttttc gcttgaataa cccagggaat
    18241 gtaaaagcaa aaccgtcgga tattttttgg cattttcgat ttttaaataa ctcaaattag
    18301 cctttataac acgtgcatct ttagtttcaa tttgaaaatc tttgcttatg gttttgcaaa
    18361 aagcattttg tgaagacaaa aacactgcaa taaaaacaaa aaaccaactt aaaatacatt
    18421 taaaaaagtt attcacctat tcacctattc acctattcan nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnt attcacctat tcacctattc acctattcat ctattcatct
    18601 atattaccat atattaaaaa aagttacaat tttgtaaatt taattaaaat ttttgaataa
    18661 aaatgccgtt aaataacata tacgaaaata gggaaaatca tgattgtaat tgatgcagat
    18721 atttattcat tagcaaaccg attcggcata gcacagagca aatttactca atatgccaac
    18781 aaatccattg atgaaataat tgaagcagaa gcagctcagg gaaactctgc tgctgcaaaa
    18841 ttcagcagag aagttttaaa agactcttcg gcattggttg aactttttaa actgtcaagc
    18901 gtaggaaaca gatttgcaat attacaaaat ttatccgaac aagaacttga aaaacttctt
    18961 ccttttttag aaaaacaaga tttaataata ggtctgaatt attttgacaa agccaaattg
    19021 cttgaactta tgggtcgttt gcccgcagaa cagcttgtta aatatgttct tgaaatgttt
    19081 cctcctaaag aaataatgaa gctgatgccc gctgagagca tggataaatt tttaaccctt
    19141 ccggatttgg acaaaaacct tgtgctcaaa catcttatgc aaatgaaacc tgagtttttg
    19201 gctcaaatga ttgaatctgt tacgggaaaa cccgcaaaaa gtcttaaccc tcaggaattg
    19261 gtaaaagaaa tagcctcttt gcctcaagaa aaatataaag aagctcttgt gaatattccc
    19321 gaagaccaaa aaagaaaatt tattttagcc atgattgaag aaaaacctaa gcttttggaa
    19381 aaatttccgg cagaaggttt tgttaaagtt ttgaatgaaa aagaaaaacc cgatttaata
    19441 aaagcagcaa aagtcattga gcctgaattt ttggtagaaa tgctcaaaga actccctcaa
    19501 gaccttcttg ctgtaatagt aactcaaatt aatcctgaaa tatttgccca agttctgatt
    19561 aaaagctatc aagatatttt aagaagtata ataagcactt aatattttat atttgatttt
    19621 tcgcttaaaa attccaacaa aaattccagt gctttttggg taaataaaaa tttcattttt
    19681 ttgcgcgaaa tttttgggtt aagttcaaat tttctcactg cagatgaaaa attgcttttt
    19741 attgctacaa aaagcagccc ggcatctttg ttttcattta tccctcccgg tcctgcaatc
    19801 cctgtagttg ctatacttat gtcacagcct gttttttcaa aaagtccttt aaccatttca
    19861 agagcacact cttcgctcac cgcaccatat ttttcaatgg tttggggggt tacacttaag
    19921 agttttattt ttgcttcatt agcgtatgtt atataatttt cttttgtata tgcagagctt
    19981 cctgcaatat cggttaatct tgagcttaaa agcccgcccg tgcaggactc tgctacagaa
    20041 agagtcattg agtttttaag caaaaacctt gcaacttttt tttctgtcct tgcggtgatt
    20101 tttttataaa tcaaatctaa gctttcaaaa aatttgttca tgaaatttaa tgtaaactta
    20161 aaatgtaaaa agtcaatgcc tatttgaatt ttcatggcaa tttcttgtct taaaatgttt
    20221 ttattatatt atgcagatta atttttaagg tcgggttttt aaaattggga ttaatatcaa
    20281 gtatatctga ctttctcagc gaaaaggctg gaaaagtttc aaaaaaaatt cttagcaagg
    20341 gtttagcaaa ggttaaatcc gattttgtgc gttccccaat aaatgactgt tttgaaagta
    20401 cttcacaaga agtttgctca aatgtaaata aaatccaaat cgaccttaaa aattttaact
    20461 taaaaaaatt ccttaaatca cttgtggatg aaaacggcaa acgtttaata gataaagcaa
    20521 aaatctctgt aatgaaaagt aattcaaaat attacggaac aaatgaagcc ctgcctgctt
    20581 ttataaaaaa tcttgttgaa ttagatgtct ttgaccaaaa acaacttgca gaaataatca
    20641 aagaattagg aaatagcaga gatttaagac aacatgccga agttttgaaa gaacttgtta
    20701 aaatgaaagc tttgacaaaa aatgccgatt ctgaaatttt ggagtggttt ataaaacaaa
    20761 aaacaaatta tgcgctttct tttgacaatg ttcagccaag aaaaaatctt ttaaaaattc
    20821 ttaattctat tgaagaaatg tatcccgaca ccgaaaaatc gcaaagactt gtaaatcgct
    20881 taggtaaaag cgggatgaat gaaattttaa gatactttga tgatgaattt tgtctgcccc
    20941 aatacgaaga gcttttaaag accttttcaa aaaataaaga tataaattct tctgctatga
    21001 caagaacctt taggcacaca ggaacctcaa ggggctttga aaaacaagaa atgataaatg
    21061 caaaaacttt gtttgtgaat ttattcaatg attctgaaat tgtaaaaagc aaacttaaag
    21121 gtaatttagc agatatattg tgctcaattt atgatgacaa acaggcaaaa ggaaaaatgg
    21181 agcttttaag tgaaattttt aaacatccga attcagaaaa tttaaattca aacagtataa
    21241 acaaccttgt tgcaagatgc tttaacgaag aagcaatggc ttcaaatctt gattttgcaa
    21301 aaagccttct gaattcaaaa agattagagc caagaagttt tgacagtatt atttatcata
    21361 taaatgagaa tgaatggcct tatgccaaag cccctgcaaa aacagcaaga ttggacttga
    21421 ttaaattttt caatgaaatg actgatttgc caaaagaaat tacctctgaa accttacaat
    21481 caattattga atataacagc aaccccaaat ttgccaaaga gtatatggat tattttaaat
    21541 cggtagcaaa aataaaaggg cttgacgccg atacattaaa ttacatagct aatccaagaa
    21601 atttatctta taaatacaat ataaaagata ttatgaaaat tcgaacagaa cttgccgatt
    21661 ttgcagctca aaattcacaa aagtttgaag gcattaacaa atataatata cagaaaattt
    21721 ttaatgaaat agaaacaaca gaagatgtta atttagtcaa agattttata aatttaactt
    21781 ctaaattaaa cccgcaaata tcaaaacaaa attggtttaa atatcatttt gattttaact
    21841 cattttcttc cggtaaattt cttgatgaaa taaagcctaa tacaaatatt ttgaaaagcc
    21901 ttgaaaaaat tgtcggcgta gaaagtcttg acgaaaaatt actgcaaaat cttttttaca
    21961 aatttcgtaa tccagaggat ttaattaatt tagacaaatt tagtgcctca ttttctaaat
    22021 taaaaaataa ttcgggtcaa aattttcaag aaatcttaat aaatttttta aagaacgaaa
    22081 attccgactt aagccaaaaa atgaaaataa agcctcccct aatagatgct ttatctgatt
    22141 ttataaaaac acaccccatt aaagaagaat ccgaaaggga agcttttggg caaatagtca
    22201 aaagaatttt aaatgaagat tctcaagaaa aactaaattt ttattcggat ttgtttaaag
    22261 aatttgaaaa agctcctttt aaatttaatt atctgacata ttcgcttgat tggagcgaaa
    22321 accataatta taatattcct tctataaaat ctaaaatgaa atttttagaa tatctagaaa
    22381 aatctgaaat gaaatctaaa attaatgaaa atatgtctga aaattttatt ggagttttac
    22441 ttaaagaaat tgaaactgat gaaaatatta attctttttc tgaaataatt aaacaggttt
    22501 ctgaaaataa acaaattcat gaatttgttt ggtataaaat attatcagag cttcataact
    22561 ctgaacctaa agatttgttg gaacttttta atatcagatt aaatttttat aaaatgattc
    22621 aggcaaaaga accaaacaat ttatggcaga taatctcgga tattcacaat aaaaaagact
    22681 atcaggaatg ttgtgaaata ttatctaata tcgaaaaaca tatccattcc aaaagtgcag
    22741 ataattattt aaaaaacatt tttaacacaa tttattgcaa agaagagtta acaactttca
    22801 ataaattgat atccattgaa aaaataaaaa acgattctca aatatttgat aaaatttgga
    22861 ataaaagaga tattttagca ggtaaaaaaa accttttcga tattgattac tgtgtttctt
    22921 taaaaaatag tttaaaatca ttaaaacaag aacttgatgc cgaaaatatt ttaaaattgg
    22981 agcaggcagg aattagtatc gattcaaggc taagaagtat tgaacaattt ttgcgcaaag
    23041 ctataacgcc tatagaagta cctgttgaaa gcatgagact ttttggtaaa aactttttgg
    23101 caaattatcc tcaaacagaa aacatcttaa aaacttttga ttttacttct tttggaaaag
    23161 aaggacttcc gcttaaatat tcaagaaaaa gttttatggc agatttaagc caacttctaa
    23221 aaaaagaatc tgtcgatgaa aaacttaaaa tatttgagaa aatggaaatt cagccaaagt
    23281 ttgacaaggc gggaaatatt tttgcctacg aaggcgttcc taatctcaaa aatttcacag
    23341 ccgaaaatga tgcccaaaca aaacttatag atttaacaag aaaatttatg cttgaaaacg
    23401 aagtagtatc acaaaatccc gaactcaata aatcaatgaa ttctataata aaagcttttc
    23461 cggaatatct taactttata ggtaaacaac agcagccaac tcacgcttac agcacagata
    23521 tccacattat gaaagtatta caggaagcga tgaatcatcc tgattattca aaactttctg
    23581 atttagataa aaccgttatg aagttttcca ttttattcca cgatatagca aaaatagaag
    23641 gcactataga caaacagcat caaaatgttt ctgcccttta tacaaacaat ttgcttgaaa
    23701 aaatcaatat gccaaaagaa attaaaaaca ggattgttga aattgtaaaa aaccatcact
    23761 ggtctgaaga atttaataca ggaagtgcgt cagccgaaaa tattgcaaca agattcagaa
    23821 accctcaaga ttatcttatt tcaaaaatct ttgcccaagc cgatttgaaa ggagtaaata
    23881 aagattttta tgacatttat tccagtgctc tttcacccga aagacttgct ccaatagaaa
    23941 aagctttgaa tgatatttat aagacgggaa atgttattat cacaagcagg gtggttaccc
    24001 cttcaaaaat tccgcagata cttcatcagg gcaaaactta taaagtaata aatatgtcaa
    24061 atcttgcaga taatcaagat atggcacagt ttgggtttgt tccgggggtt aaaaaagaaa
    24121 atattagatt tttaacccac tttattcctg ataatgcaat ggctgaaagt gctcaaaaaa
    24181 ttgatattct ttcggatgtt tcaagacccg gggttttaag cacttcagtg ctttccccgc
    24241 aaaaaaacaa aacttataat tatagaaaat acggttttgt gcttgaatcc gaaaacagca
    24301 atattgcaaa tgcttttgaa atgaatcaag gttcgggtgt taagaaaaat tttgataatt
    24361 ttgtacaatt aaccacaaat aaaaattctt acagaacatt tttaagagac aatgttatta
    24421 aagaattatc tttaaaatat ccgaatttag atgaagcgga atacggtgag atttataaac
    24481 agattattgc aaaaagacat ttcagacaaa tttctgacgt aaatacggaa aaaaggacaa
    24541 taaaaggcga agatttaaaa caagctttgt ctaatgcaca agataagctt tttaacccca
    24601 gcccaactga acacaacgaa gttgttgtgt ataaccctaa aatttcaggg atgatattta
    24661 aagaaaatgc tatagaagat gttccgcagg agttgcttga ttttgcgcac gagaaaaatt
    24721 tgccgatagt attgtttggc agagactaat ataattatta caacatcttc acaaaacatg
    24781 aaaaatcctg ttgacactga attttgttta agttacgatt gtatgcacga cactagctta
    24841 tgtggtgcag gatactgtac tggcaagtgt tgaccataaa aacaacaaag gaagtaaaca
    24901 aaaatggcaa ccgctacaag acaaagaatc agagtttgtt taaaagcata cgatcacaag
    24961 ttgattgacg tgtctgcaga caaaatcgtt gaaacagcaa aaagaacgga cgctaaagtt
    25021 gcaggaccta ttcctttgcc tacaaaaaga cgtatatatt gcgttttgcg ctcacctcac
    25081 gttgacaaaa aatcaagaga acattttgaa atgagaacgc acaaacggat tattgatata
    25141 tatgacccga ctcaacagac aactgaagag ttaagtagat tagatttacc cgccggggtt
    25201 gacatcgaag ttaaattata aacttcggat tgaaaattaa atagcatttt tattttaaat
    25261 aataatgtga tctacacaag ccttaaaaag ctataggcaa aaaagaaata ggttaataac
    25321 ctgtaaatgt agcgctcgca agaagaggta tgaaattttt cagaaatttt tgaaaaattt
    25381 gttaccggga aggaaaacat aatatgacac taggtttaat aggcaaaaaa cttggaatga
    25441 caaccgtctt tgatgaacaa gggttggcaa tacccgttac cgttattaag gttgacgata
    25501 ttgtagtaac tcaaattaaa acattaaaaa aagatggtta taatgccatt caggttggaa
    25561 cggttgaagc taaagaaaaa cacttgacaa aagctcaaat cggtcatttt gcaaaaaata
    25621 aattatctaa cttcagatac ttacaggagt tcagaattga taatccggaa gcttatgaag
    25681 taggacaaaa aattgagctt tccgttttgg aaaacgctaa aaaagttgac gtaacaggaa
    25741 aatcaatcgg aaaaggtttt caaggtactg ttaaaagatg gaatttttca agaggcccga
    25801 tggctcacgg ttctaaaaac cacagagagc cgggttctat cggcgcaggc acaacccccg
    25861 gtcgtgtaat caaagggaaa aacatggcgg gcaacatggg taacgaaagg gttaccgtta
    25921 caaaattgca gcttgttaaa atcgacagcg aaaattccct gattttaatc aaaggctcag
    25981 ttcccggacc agaaggaaaa ttggttacaa tcaaaccttc aagaactaaa tggaattaaa
    26041 aagtgaactg tgagcagtga gctgtgaaca gtagaattaa aacgttaaag taggttgggt
    26101 tttctaaccc gacaacataa aacaaataag gataaaaaaa tgtcaaaaga attaaaaata
    26161 ttaagtacaa acggtaaaga actcggcgca atggctttaa acgaaaacgt ttttggtgtt
    26221 gaaccgaact tacatgttat gcatttggca ctaagaagac agttaaataa cgcaagacaa
    26281 ggttcagcgt gcgctaaaac aagagcagaa gttgcaggcg gcggaagaaa accctggaaa
    26341 caaaaaggaa caggacgtgc aagagcaggt tcaatccgtt caccattgtt tgcaggcggc
    26401 ggagtaattt ttggaccaaa accgagagat tatggttttg ctatacctca aaaagccaga
    26461 aaactggctt tgaaatcagc tttatcagct aaggcaagca atcttgttat agttaaagat
    26521 ttttctgaaa tcaaagaacc taaaacaaaa ttaatgacaa aagcactttc ttccctgaaa
    26581 gttgaaggaa aaattcttat aatcgcggat acaaaagcag cagaaaatca atatcttgaa
    26641 cttgccgcaa gaaatattcc aagtgtaagg atgattttac cttcaaattt aaacgtaaaa
    26701 gatttattag aagcggatta tgttgtaata accgaagccg ctgttaatga aataactgaa
    26761 aggttacaat aatgagcaca gaaaacttat acgacataat taaaaaaccg ttgataacag
    26821 aaaaatcaat ggcggcaaca gctcaagggc aatactcttt tgaagtaaaa aaagacgcta
    26881 caaaaattga aattgcaaaa gcggttgaat tggctttccc cggaaggaaa gttaaaaaag
    26941 taagaacggt ttatatgcct tctcattcaa aaagaatggg tataaaagcc ggacaaactc
    27001 aatcaggcaa aaaggcaatc gtgacaatcg aaggcgatcc gattgaagaa ttaatcggag
    27061 cataggcgaa cgaagttcac cgttgaaaag tttaacagtc gaacagtcga aaagtttaaa
    27121 aattaaataa aataactatt caactcttcg actattcaac caaaatcaag caaagtaatc
    27181 aaaacaagaa aaaggacatt aaacaatggc aatcagaaca ataaatccga catcaccggg
    27241 acaaagagga atgacaagaa gtgattatca ggaaatcaca acttcaactc cggaaaaatc
    27301 tttgttgaaa aaaattaaaa aaacatccgg aagaaataat accggaagaa ttacttgccg
    27361 tcacaaaggt ggcggacaca aaaaagctta ccgcgttata gattttaaac gtgacaaatt
    27421 cggaattccg gcaactgtta agaccataga atatgatcct aacagaaatg tcagaatttc
    27481 tttggttttt tatgcagacg gtgaaaaaag atacattttg acacctgaag gattaaactt
    27541 aggtgatgta attttaagcg gtcctgaagc tcaagttcag acaggaaatg ctcttccttt
    27601 agaattaatc ccgttgggaa caattattca caacattgag atggagcctg gcagaggcgg
    27661 agttatggta agaaccgctg gagcaggtgc acaattaatg gcaaaagaag gcaattacgt
    27721 tacaataaaa cttccttctt cagagatgag aatggtaaga aaagaatgtt tagcgacaat
    27781 cggggtttta ggtaattccg aatataaaaa cctttcggta ggaaaagccg gaagaacccg
    27841 ccatttggga atcaaaccta cagtaagagg ggttacaatg aacccttgcg atcaccctca
    27901 cggcggcggc gaaggtaaaa caggacccgg cggacaccct aaaacacctt ggggcaaacc
    27961 tgcactgggt gctaaaacaa gaaaagttaa aaaagcgtca gacaaactaa tagtaagaag
    28021 acgcaaaaaa tagtgaatag tgaagagtga agagaataac ttcaccgctc accgctcaca
    28081 actcacccat aacaaacaag taaacaaatt aaaacaagga gcaataaaca aatggcacgt
    28141 tcattaaaaa aaggcccata cgtagaagaa tctttacaga aaaaagtcag taagatgaat
    28201 gaaacttctg aaaaaaaagt tataaaaaca tggtcgagag catcaatgat aattcccgac
    28261 atgttaggac atacaattgc cgttcacaat ggcaaaactc atgttccggt ttatgtaacc
    28321 gagcagatga ttggacacaa attaggtgaa tttgcgccta caagaatgtt cagagggcac
    28381 tcgggctcag ataaagcaaa aagagattaa taaataaaag gacgaaaata aaatggaagc
    28441 aaaatcaaaa caaacggata taaaaatgac agccagaaaa cttcgcagaa taattaacga
    28501 agttcgtggc aaatctgtta tagaagcgca aacaatgttg cgttttatgc cgtattttgc
    28561 agcaagactc gttgaaaaaa acctcagagc cgctgttgca aacgcaaaag aaaaatttgg
    28621 agtagcacct gaagcattaa aaatttcaga aattttcgcc gatgagagtg taacttacaa
    28681 aagaggaaaa ccaagagcgc agggaagaat ttacagaaga cttaaaagaa cttctcactt
    28741 gacgcttaag gtgagtgatt aaataagtga atagaaaata gtaaatagga aatagaaatt
    28801 cccattcact atttaccatt tactatttac caaagaaaga taaacaaaaa caaacaagga
    28861 gcaataaaca aatgggacag aaaacacatc caaccggatt cagagtaggt ataatacaac
    28921 cttgggttag cacatggttt gctaaatcaa aagactatgc cgaatttatt gcagaagatg
    28981 caaaaataag aaagtttatt aaaaagaaac tttatacagc aggtgtttca agaattttaa
    29041 tagccagaaa agctcaaagc acaacagtaa cagttgtgac agctaagccc ggtattgttg
    29101 tgggacgtgg cggtcaagga attgacgatt tgaaaaatga agtttcaaaa tatttaaata
    29161 agcctgtaat cattaatgtt gttgaagtag caagaattga tgccgatgct cagttagtat
    29221 cagaagcaat cgcacttcaa cttgaaaaaa ggattgcttt cagaagagct atgaaacaag
    29281 caatgcaaag aacaatgaga gcaggtgctc aaggtatcaa agttatggta tcgggaagac
    29341 ttggcggtgc agaaattgcc cgttcagaat gggctaaaga aggaagaatt cctctgcaaa
    29401 cgctaagagc agatgttgac tacggttttg cagaagcaga tacaattatg ggtaaaatcg
    29461 gcgtaaaagt ttgggttttc aaaggcaact taatgcccgg tgaaaaagcg gacttaaaca
    29521 taaaagcggt taaaaaagcc ggaaacggtg aagaaaaatc tatgggaaga cgccccaaaa
    29581 gagttaaaac aagtgaagag tgatgagtga agggttaaga aaataacttc acagttcact
    29641 gctcactaaa acataaagct aataaaaaca aaataaagga tacaaaaaat gttaatgcca
    29701 aagaaaacaa aatatcgtaa aatgatgaaa ggccgcatga aaggcaccga aacaaggggt
    29761 gtaaattttg aattcggtca atacgcgctg caggcagtcg agcccggctg gattacaagt
    29821 cgccaaatag aagctgctag aagagcaatg acgcgtaaaa ttaaacgcgg cggtaatgtt
    29881 tggattaaaa ttttccccga caagcctatt accgcaaagc ctgccgaaac ccgtatgggc
    29941 tcaggtaaag gtaacttgga atactgggtt gcagttgtaa aacccagaag aattcttttt
    30001 gaattggatt attttgacag ggcagaagct gttgaagcac ttcaattagc cgctcaaaaa
    30061 cttccgatca aagtaaaaat tatagaaaaa gcccaataaa aacataagag aataaggata
    30121 aaaccatgaa actaaccgaa ttacgtgaaa aaacaataga agaattaaat ggcgttgtta
    30181 cagaatcaaa aaaacagctt tttgatttaa gaatacaaaa agctacgcat aagcttgaaa
    30241 atacagcttt aattgacaaa acaaaacgtt taattgctca agcaaaaaca atacttaaag
    30301 aaaaaagtta acaaagaaaa caaggagcgt ataataaatg cctaaaaagg aaaaacaagg
    30361 agttgtgata agcaacaaaa tggataaaac ggtagttgta aaagtagcat cttacaaccc
    30421 tcatccaaaa tacaaaaaaa ttatggaatc aaacaaacac tacaatgcgc acgatgaaaa
    30481 aggactttgc ggacaaggtg atattgtaaa aatcattgaa gcaaaacctg tttcaaaaac
    30541 aaaacgctgg gcagtgcttg aagtggttga aaaggctaaa tagtgaaaaa gtgaactgta
    30601 aactgtgagc tgaaaatcag ctcacagctc accgctcacg gttcactaaa ataaaaaata
    30661 aacaaatcat atcaaaggac gtgaaataat gatacaacaa gaatcaagat tagcagtagc
    30721 agataacaca ggcgcaaaag aacttttgtg catacgtgtt atgggaagct ctaacaagag
    30781 gtttgcggca gtcggcgatg ttatagtagc caccgttaaa gacgcagcac caaatatgac
    30841 agtaaaaaaa tccgaagtcg taagagctgt tgtggtaaga acaaaagccg acatcaaaag
    30901 aaacgacggt tcgacaatca gatttgacga aaatgccgcc gtaataatca acaaagacgg
    30961 caaccctcgc ggtacacgtg ttttcggacc tgttgcaagg gaattaagag ataaaaactt
    31021 tatgaaaatt atctctttgg ctccggaagt aatttagtcg ttggcgaagg atgagcctta
    31081 cgaataaatt atcccgaact cgctgaaata agttcagcat gacataataa aaaacaaagg
    31141 aaaataaaat gaaaaaacca acaaataaaa aaagtttgca agtaaaaacc ggcgacagag
    31201 taatggtaat cgcgggtaaa gacaaaggca aaatcggaaa cgttaaaaaa gttaatcctt
    31261 caaaatcaaa tttggttgtt gaaggcgcaa acttggttac aaaagcacaa aaaccaaacc
    31321 caatggcagg aattcagggc ggattaataa aacttgaagc tccacttgat gcttccaatg
    31381 taatggtagt atgcccaagt tgcgaaaaac cgacaaggat caagcacgac atagttaaag
    31441 acaaaaaaat acgtgtttgt aaaaaatgtg gtgaacagtt ggatgtgtaa aagtgaactg
    31501 tgaaacgtga gcattgaacg gaaaatctca gctcataact ttctactcac tcataaatcc
    31561 aataaattag aaaaaggacg aatgaacaat ggcaaagaca aaaagtttaa aagaaacgta
    31621 tcaaaaagaa gttaaaaagg cactgaaaga aaaattcggg tataaaaatg accttcaagt
    31681 tccaagactc aacaaaatag ttttgaacat gggtgtcggt gaagcttccc acaactctaa
    31741 acttgctgaa gttattgaag cacagcttac aaaaatagcc ggtcaaaaat cggttgtcac
    31801 aaaagccaaa aaatcaatag catcatacaa attaagagaa ggaatgccgg tgggagcaat
    31861 ggttactttg cgcggtgaga gaatgtacga ctttttacag aaattaattt gtgttgtact
    31921 tccacgtatc agagacttca gaggagtaag ctcaaaaagt ttcgacggcc gcggtaacta
    31981 tacaatggga cttaaagaac aagctttatt ccctgaaata aaatacgaag aagttgattt
    32041 ggttaaaggt atgaacgtat ctataatcac aacagcaaac acggatgatg aggcaagagc
    32101 tttgcttaca gagcttggaa tgcctttcaa gaaaggttga aaagtttaat agtcgaacag
    32161 tcgaaaagtt ttaaaataaa ctattcaact tatcgactct tcaactcaaa cataaaacta
    32221 ttaattaaaa acaaaggatg aacgcaacaa tggctaaaaa agcaatgatg aacaaagaag
    32281 caagacgcat agagcttgca gcaaaaggaa aatacccaaa agtaagacta cacaacagat
    32341 gcagtatttg cggaagacct cacggatttc acagagactt cggtttgtgc agaattcact
    32401 tgagagaaat ggctcacagg ggcttacttc ccggtgtagt caaagcaagt tggtaaacaa
    32461 gtaaatagta aataggaaat agtaaataga taaaaaacca ttcactattt accatttacc
    32521 atttactgtt acgataaagt gaacaaataa aaacaaagga cgaacaacaa tgaatacaga
    32581 tccaatagca gatatgctta caagaatcag aaacgcaaat ttggtatctc acgaaacagt
    32641 cgaaatgcca agttcaaaat tgaaagtcga attggcaaaa cttctcaaag aagaaggcta
    32701 cataactgac tattcaacaa gacaatcagg aaaattcact ttcttgacag tcgttcttaa
    32761 atatgacgaa aagcgcaagc cggttatctc aaatttaaga agaatttcaa aaccgggttt
    32821 gagaagttat tgcaaagcaa aaaatcttcc tcaggtttta ggcggtttag gaattgcgat
    32881 tgtttcaaca agcaaaggtt tgatgacaga cagaaaagcc cgtaaagaca atatgggcgg
    32941 cgaaattctt tgctacgtct ggtaaggcga acgaagttca ccgttgaaaa gtttaacagt
    33001 cgaacagtcg aaaagtttaa acaattaaat aactattcaa cttatcgact tatcaactct
    33061 tcaactaaaa aaagagcata attggtagaa aagctcaatt tatttacaca ataccgaaat
    33121 ggagaaaaac aatgtcacgt ataggcaaat taccgataga aatccctcag ggagttgaag
    33181 ttacaataga aggacaaaca gtaacggcta aaggtccttt aggtacggaa accgtcgatg
    33241 taagagaaga aattctcatt aaaaaagaag ataacaaagt catcttgaca agacgcgatg
    33301 aagaaagaaa aacaagagca attcacggac tttcaagaac acttgttgca aatgcaatta
    33361 acggagtaaa aacaggattt gaaaaaagct tggaacttca aggcgtaggc taccgtgcag
    33421 caatgcaggg tacaacttta aacttgcagt tgggttattc acatcctatc aacattgttc
    33481 cgcctcaagg aattacaatt tccgttgaag ccaacaccaa aattacaata aaaggaccta
    33541 acaaacaaac agtaggcgat gttgccgcaa ctattcgcgc aaaaagacct cctgaagttt
    33601 ataaaggaaa aggcgtcaga tacgaaggcg aacacgtaag acgtaaagcc ggtaaggcag
    33661 gtaaaaagta acgcagttgt cggcaaacga atgagcaagc cttacaaatg cgttatcccg
    33721 tgcaagctaa gcgcgacacg tgatctcaca atatattaaa aataagcccg cataaaccct
    33781 tagattttgt ttttactaag aaggtttggg tagaaagaag gacaaaatga ttaaaacaaa
    33841 aacaagaaaa gaacaagcac aaaaaagaca tcaaagagtc agaattaaac tttcaggaac
    33901 caccgaggct ccaagattgg ctgtttacag aagcacaaag cacatctacg ctcaaattat
    33961 agatgacata aaaggtgtta cagtagcttc agcttcatct gtagataaag atttgaaaga
    34021 aaaattaaac catggcggaa atattgattc tgcaaaagct gtcggcgagg caatcgctaa
    34081 aaaagccttg aaaaaaggtg ttaaagaagt tgtttttgac agaggcggat tcttgtacca
    34141 cgggcgtgtt gcagcacttg ctgatgcagc aagagaagcc ggattggtat tttaagctta
    34201 gttaatacaa taaaataaca acaattaagg cgggttttct taaaagaaaa cccgccttaa
    34261 aactctcaac tatcttattt agctcacgct acttaactgc gacaagcctt gtttggatgc
    34321 cgatttccga attaagcact ttagcagttg ccaaagccat agaacggcgt ttttttgctc
    34381 ctcttaatat aaattcgacg ccaccgtaga tattatttgt aggtgtttcg attttcctta
    34441 acattaacat atccctttca acaactatgt atataaggaa tatcgggcag gattaaagaa
    34501 aactttagta ttttaaaaaa tagtttacat aaagttacaa aatggtgaag ggtgagcggt
    34561 gaactgtgaa cggaaatgta aagagtttgc ttgataaaaa aactttattt ggtaaaatcg
    34621 gtatagtaaa tagtaaatag taaatagtaa atagtaaata gtaatttcaa atacaatatt
    34681 tttatatttt aattctattc accattcacc attaactatt cactaaaaat gcgcccgtag
    34741 ctcagctggc ccgttgaatc ttttacgaag taaaagttca acaaaagtta gataacgctc
    34801 tatccagctg aggggcaagt gaaaactaaa caaaaaaatt tttgtagaaa aacaaaaagc
    34861 aatagtctga aaagtctttt tgcctacttt ttctacagaa aaagtaggcg cccgtagctc
    34921 agctggatag agcgtttggc tacgaaccaa aaggtcggga gttcgaatct cttcgggcgt
    34981 acttttttta tcaatacttc gggagtcccg cgcatgcgat tttgttatta ttcgcatgtg
    35041 ctcataaaca aaatctgcat tcccgacccg tcaaactacg gctcaaaaat tcgccttgtt
    35101 tgtcgtgcga atctcttcgg gcgtactttt ttatcatgta ggttgggctt ttagcccaac
    35161 attataaaag gttttgggta attgtagggt gggtttgcta tcccacattt tatattgtcg
    35221 gcatggcaat gccgacctac attatttaaa atattttttc tcaaaatcgg ctaaaaattc
    35281 tgctaatgtt tggttaaagc cgtttgctat ttttaccatg ttcattaaac ttatggttct
    35341 tttgccgttt tcatatcggc ttaatgttgc aggttctaaa tcgttattaa aggcaaattc
    35401 attaagtgaa atgcctgttt caaggcgtct ttgctttata tatgaagaaa tatttttata
    35461 gtgttgcata ttggtaatta ttaaatataa ttacttatat atcattacca aatggtaata
    35521 ctaaaaacat tattttggag agaaaaaatg aaaaaggtaa aaatctacac gtgggcggat
    35581 aagacacccg aatttattta cagacaactt gaaacgatta ataagtttgt caaggacgaa
    35641 gattttgaat ttattgtttt taacaatact ccaattaccg cttacgacag gcgttacaga
    35701 attaaattta tctgccaaaa actcgggata aaatgccttg atgtgcgttt ccgttcgttt
    35761 atttcagggt ccgcacaaat ttgtggctgg ggcatacatt gggcttttca cagatttttc
    35821 aggtgggaaa aagatacaat tcatgttgtt cttgattccg atatgttttt tataaaagat
    35881 ttttgtttca atgaatatct tggtgacaac gatatttgtg ctattcatca gcgaagagaa
    35941 aatatagaat acccttggaa cggaatcatg atttttggcg gaaaccttcc cgataagact
    36001 tcactcgatt tcaggctcgg gacaatcaac ggcataagaa cagatgtcgg cggaaaaatc
    36061 tattattggt taaagaaaaa tcctgatttg aaaataaaat ggatgggaca tacagggcat
    36121 ttggatattt caaaatcaat tatcttgccc gaatctttgc gccatgaata taaaccagaa
    36181 tattgctttc aaattattga aaactccatt cttcattaca gagcaggctc aaattgggat
    36241 aagtgtcatt tttcttttgt tgatgaaaaa agggcttatt ttgataaact tttaaatgca
    36301 cttgtttata acggtgagaa aattgaagtt agttctgaat ttaacatata tatgcaatag
    36361 ttttaatttg agtttaataa aaaccccgtg gaggcacggg gtgtgaattc caaatatatg
    36421 ttaaatgctt attatctcat agccaattgg ctgtacattc cgtttgctaa aacttctttt
    36481 ctgattaaag aattgccccc tgctttatat aagttactca agtaatctaa tcttactact
    36541 aactttgaag agcttgcttc tgttcttgcc aatcccggat ttcttgattt gaaataaacc
    36601 catgctgcag cctcatttgt ccatgccctt acctcttctt caattgagca tctttcgtcg
    36661 ttgtgggtgg tttcgtgtct gattaagcag gcaatagctt ctgcgggagc tgttttgaat
    36721 gatttgctta tgtaaattac cattttgccg tcttcgttta ccacggtaac cgcgtttgcg
    36781 ctcatataat ctattgatat tgcagataaa tttgcaaact taacctctat ggcttgccct
    36841 gttgaattat ccccttgcaa aacttttata gatgaggtca tataagtgtc ttccattaaa
    36901 gttattgccc tgtttatcaa agcatcgctt gtgtttgccg aataatcagc cgcaactgct
    36961 ttgctgccga caaagctcaa caaaagcatg acaaatacac ataaaataaa gctcttaact
    37021 cttaacatac acatatctct gactaggact tcacattttg ctttatcggc tctttatatt
    37081 gccttcttta gcaaatcaga gcatatgtta atatttgttt acataataaa gtatggtaaa
    37141 tagaaattaa tagttgaata agtgtaatta taagaagata agatgaatag atgcgtggat
    37201 gcgtagaata atctgctacg cctcttggca tcttcgcatc ctagctcttt ataagctgtt
    37261 cagctatttt attgattaat tatttcat
//
LOCUS       DAOQAU010000011        36254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1055_length_36254_cov_6.053676, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000011 DAOQAU010000000
VERSION     DAOQAU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 36254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36254
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1055_length_36254_cov_6.053676"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKI94_03170"
     CDS             complement(<1..314)
                     /locus_tag="PKI94_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25770.1"
                     /translation="MSMQILANFNNKYAGVPLSYTSDVNKGKKHPKSAIKQPEAPKNV
                     KRGVFLTTALGVATGVAIALKRGVCIKDPKLISGKLKFLNPLMKVDYDWKNIIIMAGS
                     SI"
     gene            complement(405..890)
                     /locus_tag="PKI94_03175"
     CDS             complement(405..890)
                     /locus_tag="PKI94_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25771.1"
                     /translation="MAEKTVFDKKLNLNNITVMSAFSNESDMFTSLSTTALLAKNSVP
                     YSHKRAADNYVIIKRIYKFQAVQSRITNQEIALLKKYDFNLLEHSTIKQINEELAYLK
                     KWAVSSNEQLASDADEIIQIRAKELKFIVASNYSAVSEEIYDGYKALERYFEEITKFY
                     R"
     gene            1031..3232
                     /locus_tag="PKI94_03180"
     CDS             1031..3232
                     /locus_tag="PKI94_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNW25772.1"
                     /translation="MNKPQSFEAMGEFFSSSKFLNLCAFFGFALLMSIILASQNLLFQ
                     NIIENGISKKDIIAQRTITVIDTKRTEQHKREISQKVEPILAPAEDSFIKNNLETLES
                     SILQIRKKDVPMSVKTSELSILFDISEGYRKEFVINYLLKTNENSVRQVFDKASLVLT
                     NILSVGISEKDYENNNIRKIIHRNMVSNIPKSQSTVITELLEQVIVPNLIIDEDATTT
                     AKKNAQDAVKPVEVVFEKGDKIVFEGEPVTQLKRDALRKAGYNVLEMNFKGFLGIFII
                     VLLTSLAFLKYLRTFEKQYIESSRIMIMGALSLAFALISILIPTGFSPYMLPMPAFII
                     ILSIFTTPRIAMMLASLMLITLTLTLEYNIQFVLAFALINLISMITIAQVKFSRRFDL
                     IKTGIDIALTSVVIVVSIYLLEKCLIEVDNALIVTDTAITFINCLLSGVIALGLLPLL
                     ESIFKIITPYGLAELADHNQPLMKRLQFEAPGTYHHSLMVSNLCEAAAESVGANPILA
                     RVGAFYHDIGKLKRPLFFVENQSYFGIENPHAKLNPRLSKMVITAHPKDGVELAKEYG
                     LPPIIYNFILQHHGEGLASYFYTQAVAEEGAENVKEEQFRYTGPKPNMKETAILMIAD
                     AVESATRALKNPTPEEIETIIDKIIIERLNDGQLSDSPLTLRDIKTIAATFNRILRGM
                     QHNRIKYHEDIVHELDKTKINIPMPDKVIDKELDEKIRQLEQGGNEKNDKK"
     gene            3210..3716
                     /gene="ybeY"
                     /locus_tag="PKI94_03185"
     CDS             3210..3716
                     /gene="ybeY"
                     /locus_tag="PKI94_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNW25773.1"
                     /translation="MKKMIKSNIFSENIHPSIKINEKYFISAAKNILKYYLQDNNIFD
                     KSCLAKKKFKTLSFDILYCDSKKAHEINKEYRKKDYPADIITFAIFADSNEKFVFDNE
                     INLGEIIISLDNATQEAQKKGTPLKEELKFLISHGIMHLLGFDHETNEKYNFIVTAQD
                     NALKNLKG"
     gene            3718..4095
                     /locus_tag="PKI94_03190"
     CDS             3718..4095
                     /locus_tag="PKI94_03190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNW25774.1"
                     /translation="MSKYKSKGGFSGTCQNAFKGIRLALKSERNIRVHFSIALLVTAL
                     GIILKFSPLEFSILLFTISLVIIAEMVNSAIEFSLDSIYKNKYSKMVGMAKDIAAGAV
                     MIATFISIAIGILLFGSKLFNLF"
     gene            complement(4098..5492)
                     /locus_tag="PKI94_03195"
     CDS             complement(4098..5492)
                     /locus_tag="PKI94_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25775.1"
                     /translation="MFVDKESHSNVSFEGTGSLLIKYHKYSEKMGQEILQLMEQKVGK
                     LSGDIKSFLKSNGALNEAGDLVGLKPKSITILDGNEYFDEEILKDASFIFQRGGSLDK
                     VIEVDKIKIEGGQNVGIRAKFVSILGDFKSRFIFADDLKARNSKLDSYVEVKNAEFYN
                     SSNLKKMSVSEDLDLYGKSKVRNSTVGDLYLDNASFADNVVVKNNFSAWENSRALRLK
                     IQNNADLHDSAVLSMSGVEHVLNTCNKSRVSHSVAGSFYAHNNSILQKVVALRHIGAG
                     HSSIVKSSISKGDLVAGNSASVSDVTVKKSSLIGQNAFATKLKTNTLKVIDSARVENS
                     LINGDLSLRGDSVAVNVKVGDCANVLDWAKLQKSEVKRGLTLHDNGQASDIWVQQEGL
                     NVNDTAGIKNIVAPKYYLYDRSTIGGKIQGKINYIDHRVKINPDTKFDAFARHSLPIT
                     RFRAKFLSKYSLKV"
     gene            complement(5585..6535)
                     /gene="thrB"
                     /locus_tag="PKI94_03200"
     CDS             complement(5585..6535)
                     /gene="thrB"
                     /locus_tag="PKI94_03200"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNW25776.1"
                     /translation="MKISVKVPATTANIGSGFDCLGMALPIYNTVTIEETVLPGTGIE
                     INVMSEGDTEEDFAMEHIPTDENNIVYKAVELLYNSIGQNPAELKITIKSQIPIARGL
                     GSSAAVIVGGLLAANELLGKPADEAALLSIATEVEGHPDNVTPAIVGGLVLTSQEEDG
                     SILYRKLKWPEEWHITVCIPDFELATEIARSVLPKEVPMQDATFNARRLAMFVEAIHS
                     KDAELMKLALQDKLHQQYRMKLVPGLCEIIENLKHEENVLGCVLSGAGPSILIISQKN
                     NLEKIKSIVSETWADLNVKTDIKTLKVEENGAQILEASLV"
     gene            6709..6909
                     /locus_tag="PKI94_03205"
     CDS             6709..6909
                     /locus_tag="PKI94_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25777.1"
                     /translation="MSTVEYPAMIYKNSRNNNFNANCIVKNVIGFGKTEEEALNNLKA
                     SLQKDTNIEIIIKPMYGLSIAQ"
     gene            6969..8318
                     /locus_tag="PKI94_03210"
     CDS             6969..8318
                     /locus_tag="PKI94_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNW25778.1"
                     /translation="MIDESCNNALSEIKSRAKHVIDSTSLYKYKGSVSKLLGLTVEVK
                     LPGLKIGDLCYIENMHGEKKAAEVVAFKGDVAQLLLLYDGAGIGQGSLVQSTGGPIML
                     PVGEFLLGRLINPLGEPIDGMPLDTTEAKWVSVENSPPDAFKRPLIKDMFSTGVRAID
                     STLTFGCGQRMGLFAGSGVGKSTLLGMMARNSDADVNVMALIGERGREVRDFIENSLG
                     EEGMKKSVLVCSTGDQPPLIRQKCLLSATAVCEYFRDQGKKVFLMTDTVTRCAMAGRE
                     VGLSIGEPPTMKGYPPSIFSWLQKALERTGNSPEGSITALYTVLMEGDDITDPVVDTV
                     RGIVDGHVFLSRKVAEMNHYPAIDILGSISRLATEITTEEQQEAAAKMRKILAIYREN
                     KDLIDVGMYQPGTNPTLDVAIEMMPKVNAFLQQKTSESVNMENTIQTLISMMSNVEI"
     gene            8539..8841
                     /locus_tag="PKI94_03215"
     CDS             8539..8841
                     /locus_tag="PKI94_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HNW25779.1"
                     /translation="MLHFAQTAIHYINSSNFSYYEILMLVCFGISWPVALLKSYQTKS
                     VKGKSFLFLLLILLGYIFGTVHKIMYSFDYVIWLYIINGAMVLADMALWILYRNND"
     gene            8848..9774
                     /locus_tag="PKI94_03220"
     CDS             8848..9774
                     /locus_tag="PKI94_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25780.1"
                     /translation="MKKILLIILSVIIFSPKVFAESENIALLTQKGFFDKIKTTSQNP
                     YEEIRDTFYLHTKFSNEYKFEQLKDLYAPNYYNADGFNRENYFDLIKKTWENYPGIKY
                     KMEIIDIKISGNYATVYLEESARADTASAGGILRDKGYLQSNSDTIYFLEKIANEWKI
                     TSDNIIFEKTYLTYGSASYCSFNLNAPSKIPMNTEYTATLNIKSPPDTIVLASIGREK
                     VTYPQIQAEEVFRRLPESGILERMFISNNENLNEFAVASFGVAKLDLSRGSDIKINIT
                     GMGFAMSRVNVISAKLENNKNDKKKDSENDKK"
     gene            9761..10234
                     /locus_tag="PKI94_03225"
     CDS             9761..10234
                     /locus_tag="PKI94_03225"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNW25781.1"
                     /translation="MTKNSKYTYFLISFLFFSFIDFYFSNLILKKIAESGNFLKYGIN
                     FIYTKNYGAAFSIMQNSRLFLIIISFIALILMCYYIVKNIENILIKELFFITILMAGI
                     FGNLYERVVFGYVRDFFDLTFINFPIFNISDIFINIGVFGIIVMIILTKKPIKLL"
     gene            10231..11154
                     /locus_tag="PKI94_03230"
     CDS             10231..11154
                     /locus_tag="PKI94_03230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNW25782.1"
                     /translation="MKELYFTVEESDEGKRLDVFLSNVFDKISRAKIQELINNNKVSV
                     NQNFKKNSYKLKINDKIFVQFEEQEQEIIICPQDIALDIVYEDENMLVVNKPSGMLTH
                     PTSTEKENTLVNALLFKYGQNLSDINGEFRRGILHRLDRNTSGLLMVAKNNKAHEFLA
                     NQIKEKTALRKYLTIAQGNFNTDTGIINLPTSRHPKFPHKRAVVEEGKPSITEFSIIE
                     RFKGYTYLELTLKTGRTHQIRVHLSHIKHPIINDSLYGAAPFAVKTQEQVLQAYKLIF
                     TKPFSCEIIELEIEPDEKIKKVLGYLRSKKL"
     gene            11151..11450
                     /locus_tag="PKI94_03235"
     CDS             11151..11450
                     /locus_tag="PKI94_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25783.1"
                     /translation="MKIFLRTIVVILFVLSLISFYNYKFDYNSLEILLIGMFITYLWY
                     VPYRREISKKIKEYQNRLEKTSVSAETDSSKVEVLEAKIQSLEKALQSALEKNKD"
     gene            11577..12212
                     /locus_tag="PKI94_03240"
     CDS             11577..12212
                     /locus_tag="PKI94_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25784.1"
                     /translation="MKINLTPNCISKKINNRSIFTNNASSSQFRNKISFKNLEEFEND
                     KWEDRDTNNDYSVVYKSAEETKKYIEIKKNKYGYIDILLTLLNQNNQGENVLHQTSKW
                     SADNNKINDNVKKTSIILNALKPADKIKILASKNYMGETPIDVAIESFGYPSDILEMF
                     IEEIANALEKDSWPDINSKTDFRAIGKKIIEDFPDETNFEQCKKLESQLKK"
     gene            complement(12235..14190)
                     /locus_tag="PKI94_03245"
     CDS             complement(12235..14190)
                     /locus_tag="PKI94_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW25785.1"
                     /translation="MNKVSMTTQNRLILTGLVLSTLLIILIAVLASLNIQSKLNEGYQ
                     NFGKIISKTLAIESVELLKDIPEYAKYSTLKYHTQSILNSNDDIAFIEFADNKGNVIY
                     SSKEDFPKQAQDSKVAVSSPMVMKSLGQSTSIGSVMVGLSGNIINQISSTTRASLLFV
                     FVVAWAVFAVVILVNTYLITRELRILHHGVRKISTGEFGYKVQGKGVSSEVQELFDAF
                     NDMSSRLHLYEEQNVEQLTLERNKMEAVLMSIANGVVVCDNYDKVVLVNSQAKKLLEV
                     DEKDILNSKIQLYCDSNGELCFKEKIEQFKDTPLDVMEKKPLAFNIEVDKRVIKAIIS
                     PMFSRNKDYVGYIIVLIDVTKEVEMDKIRGHFISNVSHELRTPVTVLRSYIDTLYNFG
                     SDFDFNTQKEFIGVMNQEIIRLNRLVNDILDFSRLESANVKLEKTVTNIVQLIENAVN
                     SMQVLAQEKNLTFSIMKEPDLPEIPVNEDSIERVLKNLLSNAIKYSPENGRIKIRAER
                     ARVGDFVEISIEDQGQGIAPEHLGKIFDRFFRIENDTHTIKGTGLGLHLVKIAIEKHH
                     QGQVFVQSKLSEGSTFGFRLPVNPISQEDEQEHRPLPHQDEEIEDSAAFQEDEIDFEK
                     VQIENQPQSEINADGWEISVGVEKKEV"
     gene            complement(14213..15586)
                     /locus_tag="PKI94_03250"
     CDS             complement(14213..15586)
                     /locus_tag="PKI94_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNW25786.1"
                     /translation="MYNVAIIGAGPAGIFTALEIMKLKPDWKVVLVEKGKKIEKRKCF
                     LREGHKSCPGCKKCGLLCGWGGAGAFSDGKLTLTPDVGGNLLDYMERGEVEKLIDYTD
                     KIYLDFGATEVVYGTNKEDFADIAHKATLAELKLIHSPVRHLGTERSLHVLKNMQDFL
                     TDKITILYDDAVDKLIVENEQVKGFTTKGGEKITAQYIVMAPGREGADWLTSEFAENK
                     IGMVNNAVDIGVRVELPAPVFEPITEKLYESKLIYHSPTFGDEVRTFCMNPYGEVVSE
                     NYNGILTVNGHSYATRKTANTNFSLLVSEKFTEPFKEPIAYGQHVARLANMLAGGVLV
                     QRFGDLLDGRRSTPERIAASTITPTLSSATPGDLSLVLPYRQLLSIIEMLYALDKIAP
                     GTASRYTLLYGVEVKFYSARVKLTKELETEGVKNLFTIGDGAGVTRGLIQASASGVHA
                     ARIICSR"
     gene            complement(15662..16267)
                     /locus_tag="PKI94_03255"
     CDS             complement(15662..16267)
                     /locus_tag="PKI94_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25787.1"
                     /translation="MQVNSVQRNNPNFTSVIPVKVYIDGLPSVDRKNIQKAIKALSNI
                     LVHGSKGKEQSKNIIKIFINAVKDFKFLPGVEEPGRCLRNYIFKKEVDAYLFTGSEAW
                     NIDALARKIGPAKSKGLDAYGTSKTFEASARGNSYFDKILEYIKNPKLRLKEVKETGN
                     GPMIGDPMRLNVFMTSSGEYGKSNFKLNLENIGFENIKKQA"
     gene            complement(16359..17870)
                     /locus_tag="PKI94_03260"
     CDS             complement(16359..17870)
                     /locus_tag="PKI94_03260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNW25788.1"
                     /translation="MKRIIAVSLISLLLIIVSFKSIVPAVFLSIGKSFYESKNYTGAH
                     NILKIALDFNNKNRDLRYYYVQSLIKLEPTIEMQKELYDFANFKSDDSASLIADKEVS
                     NIRKQAYFNIGENYIEQVPFNNRILRWDTTKFPLNVYIENTVKNLPQYYIEQISSAFS
                     QWQSSTAGLIKFKTVQNKNEAQITVKIVPLDAMSMCSKEDCKYVVAYTTPVISGDFLK
                     RMDIIFYDSNNLAQHFTPREVYNVSLHEIGHALGIMGHSYNKDNLMFMENNLDKTYDR
                     HRSVFQLISPVDLDTLQLLYRLAPEITNTAPNKLITKGFIYPPIVMGSDQQISSRGIM
                     EAENYINSAPELPNGYIQLASVYAGMKEYNLAIDALNKALTLSSNDSERYVVYYNYAV
                     IYMNIQDWNEALKYADMAKGMSPESGSDIDGLIAAINFNKGNKDFAKQAYIDTLSKHP
                     EKIVDSINLAKIYLREFNFAQAGKVLNRLIQANPNARIDSRLKPYGLLIFLFK"
     gene            complement(17875..18396)
                     /gene="scpB"
                     /locus_tag="PKI94_03265"
     CDS             complement(17875..18396)
                     /gene="scpB"
                     /locus_tag="PKI94_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNW25789.1"
                     /translation="MTEGLKSRIEAVLFTTAKALKVKEIAEILESSFEETEEALLDLI
                     MDYSSRDGALEIDDEEGYILQVKSEHMDIVEKLCPVELKPAVLKTLTVIALKEPIRQT
                     FLKDLRGSNAYEHVHELVDKGLISRNKDKNGRSFNLKTTQKFAEYFKIKGDAKNLVKI
                     LEIDKGVKDNEEE"
     gene            complement(18393..19223)
                     /locus_tag="PKI94_03270"
     CDS             complement(18393..19223)
                     /locus_tag="PKI94_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNW25790.1"
                     /translation="MTEAIDYNYVNTSLKGQTDVNFEFVALNPVSQEMDGIEILVSMA
                     KTGKVNPWNIDIVDITDKYLAQLFQMKAQNLKLTGRTLLFAAILLKLKSNVLENIDIV
                     EFEGSYEEEANDLDYNDDFDNGYEDYGSNVISIDEVLQRRTSVRLNRNRVVTLKDLIR
                     QLEFYEMIDRKQSLKNAHERAKRRVRSFANLSPDDIVNLAHDEYIEDSVQKLRENLEQ
                     IFKKEKRIELNELTLLGMDKVTAYIALLFLSAESDYELVQKEFYSDLYVTRSKWLKNK
                     "
     gene            complement(19240..22734)
                     /gene="smc"
                     /locus_tag="PKI94_03275"
     CDS             complement(19240..22734)
                     /gene="smc"
                     /locus_tag="PKI94_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNW25791.1"
                     /translation="MYIKQLEIDNFKSFANKMDIPLLKGFTTISGPNGSGKSNIIDAV
                     LFALGLSTSRTLRAEKLFHLISTHTKRNDALVKVTFGGVEGGKDLVVGRKIKKSSQGY
                     ISTYYLDDKVVTLSEIHSILEKHNITPNSYNVIMQGDVTSITNCSSNERRKIIDEIAG
                     VADFDRRIEQATNELETVEKRVEKSSLILSEVDIRLEQLKEEREVALKYQKLKDEKIN
                     LESQINKVKFFDIKRNLEKAHENILEFTKKKKEEEVKHKDLEERLELIKGKYQEICEL
                     VKEKGEAQQIEIKKLAEETKGAIERKQSAINYADKQIHDGLKSIENAKNGIEVQNKKI
                     ADTELKIQLKKDEIKGIEENIALQKAELNKILEEMTGLNQTADQHIEKRNQLRKDLEA
                     LQDKENNLIKEKLPLENELKNLQKELGEAQEQVEKLKDFKKNFGSNKDLISTQSEQLK
                     REMEDLKIVQQNAMHDLDTTKNEISDLNYDIQMAYRKISSMEAQKQAVEDANFGRAID
                     TVMNAKLKGVHAPLVKLGKVDKKYSTALEIAIGGRITHVVVDDEHVASVAIELLKSSD
                     AGRATFIPLNKISKAPSKLPLPKDKGVIDYAINLIDFEDKYTNAFFYALGDTLVVDNL
                     DCAKKLMGRFRVVTLDGEIVEKSGSMTGGSVRKTGLKFSQNDDEELDKFRAKLKEMEK
                     NLASLENKKIELEKKLDSVRSDYSNTMTEYNKANIEVANLVKNSEDNENLIAEKLEFI
                     KLTEPQIEQISKKLDKMESSHVEVTSSIQELQSEIERVEKLINEEDLKNLKEKTESTE
                     FEIKRLNTNLTNANNDLSEMGREIAFFKDLIKTKEEEIKNIEQSSITLEQDKKTYAEE
                     IKVLQDKLDDYEKQIEQINEKLGELLAQRDQINSELLEMETQKHLHVSDLERIAEQIE
                     SFKARRRELEPQLELAREELESSGLEVNKLEPVTISIEEITAKIQRLEKRMEDLGLVN
                     MRALTAYDDVLARQQELKEQIETLSKERKQILERMQGYENLKKETFMKTFNSINEYFK
                     EVFHKLSEGEGTLILENEEDPLSGGLTIEAQPRDKKKQRLEGMSGGEKSLTALAFVFA
                     IQKYMPAPFYAFDEVDMHLDGINVEKLSDMIKHQAENTQFIVVSLRKPMIESANRTIG
                     VTQKEKGITRVTGVKLRE"
     gene            complement(22864..23292)
                     /locus_tag="PKI94_03280"
     CDS             complement(22864..23292)
                     /locus_tag="PKI94_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNW25792.1"
                     /translation="MIRNFLKNLKNFFYTKVLKKKYYRIGQCLGCGKCCKKIYVQHAN
                     HVIQDEKEFQKLKYLHRFYTYLNVVEKDDTGLVFSCSNLDEKTNLCKIHKDRPGICRR
                     YPQEELFAMGGELSENCGYKLVPIISFSEVLNKKMKKKNY"
     gene            complement(23285..24517)
                     /locus_tag="PKI94_03285"
     CDS             complement(23285..24517)
                     /locus_tag="PKI94_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25793.1"
                     /translation="MEEQQNIAEYQVQNNGDIEGGVLEAAQRQYLNKNYSEALRLFTG
                     ILNTNASADVYVDVGNCHYMLHDEKEAIEYWTRAIELDSKCSKAYANLGNLYYKGGQV
                     EMAISYWLVALISRPEDANTCLNLAIAFNQKMMRFESIKYFEKYLKYAEDKSSSEYAK
                     VKSKITHCYNVANQYLTAGAQLHSQGNEKKAAACYFKSLANYPNLSKTNLNLGSIFFA
                     DKNLELAIKYWKVASHLDPNYAKIYSNLAISYDLFKQFNYAYCYYYRYMNFVIGDKEE
                     YYKANKRLLKLKPYLNENQHLIEEHLKKAEEHIAKSEFFDAIDEFKNYSILKPESAAE
                     YKDLIKKLESYLNPEASIIESCFNIGNDLVNQGRFSEAKPYFWRIMKLSSPQFLEFSK
                     AKAKYSQCEKAETEPFGD"
     gene            complement(24539..24883)
                     /locus_tag="PKI94_03290"
     CDS             complement(24539..24883)
                     /locus_tag="PKI94_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25794.1"
                     /translation="MLNEVKNAEDILNSQIGKKSEVSKLHTNPIKNAYMSIDKGFLID
                     ETAISNEAVSMYEKEQDIRTFTKLAMSDPNDFSHDAIISKLFAGGVEDPLSDEAMEKL
                     SSNERLLKDLFS"
     gene            25067..26356
                     /gene="serS"
                     /locus_tag="PKI94_03295"
     CDS             25067..26356
                     /gene="serS"
                     /locus_tag="PKI94_03295"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNW25795.1"
                     /translation="MTKKEKITMLDIKLIRENPEKTNELLKRRNPELSIDEVLQIDAQ
                     RRAVQTQADEMRAKRKKDSLIIGEMKKKGENTDRLQEDVRKIGDEIKLLEEKEVELDQ
                     RQRILLLNIPNTPDETTPKGTSEADNKIVSGCGEPTKADFELKAHWDIAEEKGLVDFE
                     RAVKISQSRFVMNRGKGAQLERAIINFFLDVHTKEHGYEEILPPVLVNSAAMTGTGQL
                     PKFAEDMYKCENEDLYLIPTAEVPVTNIYQNEILEEEDLPKYMTAYTPCFRREAGSAG
                     KDTRGMIRVHQFNKVELVKLTTPEQSHEEHLKLTKDAEKVLEMLELPYRRIELCTADI
                     GFSAQKCFDLEVYMPSYNGYKEISSCSNFGDFQARRANIRYREKSSGKVQFVHTINGS
                     GLAVGRTFAAIIENFQQEDGTILIPKALHPYTGFEII"
     gene            26356..28650
                     /locus_tag="PKI94_03300"
     CDS             26356..28650
                     /locus_tag="PKI94_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW25796.1"
                     /translation="MNKFPFELDEFQKEACKYISDGKSVVVCAPTGAGKTVIAEHAIH
                     LAIEQNCRVFYTTPLKALSNQKYSDFSNQYGINNVGLLTGDTSINRDAQIVVMTTEVF
                     RNMLYGTNFGSVTENMKNVKYVVLDEVHYMNDEQRGTVWEESIIYCPTNIQIIALSAT
                     VANADQLTDWINTVHSRTELINTDFRPVPLRFYYFDSSQPNTILPLLTPSRQLNSKIR
                     PEKRQFHHRGKVPERNTVRDVVRNLHEKDMLPAIYFTFSRKKCDDQMEKCSRMVLVTP
                     QEQKQIKEIIDEYLIENPYLCKNKHIEYVLNGVASHHAGLLPGWKVLIENLFKKGLIK
                     VVFATETLAAGINMPARSTVISSISKRTDTGHRTLTASEFLQMSGRAGRRGMDEIGYV
                     TVVGSQFDSPQDVAELVLSDANPLESRFSPGYSMVLNLLQRFSLDEAKELILKSFGYF
                     SSTSRLTPLLHQKEINQKAIDELSGFVCHYKLTNQDLLDYNKIRSIYVENRRIFKTIQ
                     KQERHKNRPLNAEAQEFGRKNKEMLYKMQSYKCDTCKLFKKHSKEIEVLERYKIKQTK
                     LEKDIEHSKDIFWNKFVSHRQVLKDFGYLKDDYPTEKGVMASQIRSENELFIAEIILS
                     HVLEALDPAELASVICAVTTEDLRAEIYPQIPISQAVRKTLNQIKNIKRNIDKAQKDN
                     NIETPMYINSYYSSMIEMWVNGAEWESILDQVEMGEGDVVRIFKRTVDVLRQLCVISN
                     ISEALAFTAREAINGILREPIDVD"
     gene            complement(28667..30262)
                     /locus_tag="PKI94_03305"
     CDS             complement(28667..30262)
                     /locus_tag="PKI94_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNW25797.1"
                     /translation="MKNPLISKLKIIFTNAFKTKSPDELIEEGAKSELSKTLNIFDLI
                     VIGIGAVVGTGIFTIVGIAAQGGPEGVGAGPALVVSMIIAAVACVFAALCYSEFASMI
                     PVAGSAYTYTYATMGEFMAWMVGWILMLEYAIGNITVASAWTGYLFQLLKGFSNVLPA
                     WLVSPPIWLINDYRTATIICNREGLDPNVVIPHFFGIPIAINIPAIFIVFLLTLMLIK
                     GIKESTKLASIMVGLNLTIITAFIIFGSFYIKPENWTPFAPNGFEGVFMGAFLIFFAY
                     IGFDAISTTAEETKNPQRDIPIGILGTLVICTVLYILVSLVLTGMIHYNQIDFQAPIA
                     HAMRLVGQDRLAGFISVGALAGLTSVLLVYQLGTTRILYAMTRDGFLPKSMNKIHKKF
                     HTPHVLTWVSGIVVIIGALFMDLNISAELCNFGTFTSFIIVCAAVLILRKTDPDRHRP
                     FRVPFSPLFPWLGIITCGGLMIYSMKNLKTSATIFPLWLLVGFLIYAVYGYRKQRELK
                     QDNIVDDTTTEELSTAAVDIEPA"
     gene            complement(30240..31847)
                     /locus_tag="PKI94_03310"
     CDS             complement(30240..31847)
                     /locus_tag="PKI94_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNW25798.1"
                     /translation="MTKHLHRARFSKEFLQNIVRTKNPDDMLNDAKNEGLDKTLGVWD
                     LIILGIGAIIGSGIFAVVGIAAAGGPESIGAGPALVVSMVVAAVACVFSALCYSEFAA
                     MIPVAGGAYTYTFATLGEFAAWMVGWILMLEYAIGYIAVACAWSNHFMQFLRGFSNYL
                     PYWLVNPPVWLVNDPRSAAKILTDSGVNPDTVMPMIAGIPLSINIPAILIVIAITLIL
                     VKGTKDSTKMAGIMVFIKLAVIALFVLVGAFFVRPENWAPFAPNGAEGVFMGAFIIFF
                     AYIGFDALSTTAEECKNPQKDLPIGIIGSLVITTIVYVLVAIVLTGMQPTSGGVIPAG
                     FLKAPMAYAMSIAGQDWAAGLISIGSLAGLTSVLVVLQMASTRILYAMSRDHFLPKRL
                     QKLHPKFHTPHVLTWIVGIICIFGTLTLDLNVAAELCNFGTFTSFIIVCVAILILRKT
                     DPDRHRPFKVPFSPVLPILGIICCGGLMIYSMQFLTTSSLLFPVWVGLGALIYFHYGY
                     KMNRKQEFKNITENESEIKINEEPVNL"
     gene            complement(31848..32750)
                     /gene="glyQ"
                     /locus_tag="PKI94_03315"
     CDS             complement(31848..32750)
                     /gene="glyQ"
                     /locus_tag="PKI94_03315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNW25799.1"
                     /translation="MAEKALNFQELILNLSKFWSDYGCIIQQPYDIEKGASTMNPATF
                     LRSLGPEPWNTAMVEPCRRPTDARYGENPNRLGHYFQYQVILKPSPDDAQELYLKSLE
                     AMGIDLSKHDVRFVEDNWESPTLGAAGVGWEVWLDGMEITQFTYFQQVGGLEIRPVAL
                     ELTYGLERIAMYLQNVDSVFDVKWNNELKYGEIYLQNEIEQSKYNFEVSCADSLFKMF
                     DLYQKEVENCVSKELVLPAYDYVLKCSHTFNLLDARGVISKDERINFINRVRTMAAAV
                     AKLYVEQREKLGFPLCKYEKVGNK"
     gene            32900..33418
                     /locus_tag="PKI94_03320"
     CDS             32900..33418
                     /locus_tag="PKI94_03320"
                     /inference="COORDINATES: protein motif:HMM:NF016185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25800.1"
                     /translation="MADKIIIFSGKQYSGKDTVAKIMMQEMPEYKRFAMGDVIKLEYG
                     QKKGLSYEEIEKNKPLYRQDLIDLGNWGRSQHPDYWLKKIISQPGKIMVTDVRVPHEL
                     EIFRAAGAIAIRVEASRQVRASRGELIGENDVTEVGLDHITDWGFVIENNSTYEKLKE
                     QTFEVIKQLSAC"
     gene            complement(33404..34372)
                     /locus_tag="PKI94_03325"
     CDS             complement(33404..34372)
                     /locus_tag="PKI94_03325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW25801.1"
                     /translation="MTDLLKLPLVSFVVTSFNYEDYVVKTLESIKNQTYENIEIIVVD
                     DCSKDKSVEKIKEFAQKNKRVPIKIIEHDCNKGQMAAIQTGLKNTQGQFICFVDSDDI
                     LIKDYAKTLLRVHLSTSVAFVSAQIVEIGEHEEIHTTYSVSSFQKEKSFELKSLKDLL
                     KIDVDNVDFEVFTLKKKPFGGWYWSAMSANMFRKSALDLFLNYDTPQNWRICPDKFLL
                     NFAHLLGGSAIVYAPLVGYRRHKNNAGASNQVCGEKRYHCDEVTKLNLDNNRKIRRDT
                     LNFIFKNKKSFNKAIGTRGTNGLVLQIILSYFKIDFKKVFGFLAGR"
     gene            complement(34365..35408)
                     /locus_tag="PKI94_03330"
     CDS             complement(34365..35408)
                     /locus_tag="PKI94_03330"
                     /inference="COORDINATES: protein motif:HMM:NF010613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNW25802.1"
                     /translation="MNYFFSSYLVTIVGLVLAYFWGEHVHQGSGLTTVFIAFVLSVLE
                     VSLSFDNAVVNAMKLEKMNEKWRRRFLTWGIAIAVFGMRFLFPVLVVAVFAHLGLFEV
                     VKIALTDSKMYEHYLHLTHAPIVSFGGTFLMMLFLDYFINDEKELHWLKWIEKRFKEM
                     GNIKGAETIVTLIFLFLTQHYVVQNERLYVVLAGIFGVVIYLLIDGITQWLEQKEEES
                     TKLIAQTVKGSGLMGFLYLELIDASFSLDGVLGAFALSKDILIITIGLSIGAMFVRSL
                     TMMLVEKKTLGKYLYLEHGAHWAIGALSLIMLVSTFYKVNEILTGSIGLIFIAGAFIS
                     SLIHNKKLRENVD"
     gene            35575..35808
                     /locus_tag="PKI94_03335"
     CDS             35575..35808
                     /locus_tag="PKI94_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNW25803.1"
                     /translation="MNSRAKVCDFMSHENSVKNLLKKGFNKVFTYPQSDAREVAKIMA
                     DINTDSLPVLLSPWSKKLVGIIELRKIKKFLDD"
     gene            complement(35786..36058)
                     /locus_tag="PKI94_03340"
     CDS             complement(35786..36058)
                     /locus_tag="PKI94_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25804.1"
                     /translation="MTTVDKSDVIKVAKPNVGVIVPPNDIPNPEIYSDKEARKKFKEL
                     HKDVFESQNKLKRHSDAKNFRGLLISTGIVGVFYLLAKNLNRLRIF"
BASE COUNT        12042 a         6856 c         5939 g        11417 t
ORIGIN      
        1 aaaattgaag atcccgccat aataattata tttttccaat catagtcaac tttcataaga
       61 ggatttaaga attttaattt accactaatg agtttaggat ctttaataca tactcccctc
      121 ttaagtgcta ttgctactcc tgttgcaacc ccaagagcag ttgttaaaaa cacaccgcgt
      181 tttacgtttt ttggggcttc cggctgcttt attgcacttt taggatgttt tttgcctttg
      241 tttacatcag aagtatagct tagcggaact cctgcgtact tattattaaa atttgccagt
      301 atttgcattg acatttttac ctttcacaca aaacataaga aaacaatatt aataaaaaga
      361 aaagaaaaaa aaattgcctg attgttaaaa aaaattaaca aatattaacg ataaaacttt
      421 gtaatctcct caaaataacg ttctaaagct ttgtaaccgt cgtaaatttc ttccgacaca
      481 gcagaataat tgcttgccac aataaattta agttcttttg ccctgatttg aattatttcg
      541 tcagcatctg aagctaattg ttcgtttgaa gaaaccgccc attttttaag gtaggctaat
      601 tcttcattaa tttgcttaat cgttgaatgt tcaagcagat taaaatcgta cttcttaaga
      661 agcgcaattt cttgatttgt tatgcggctt tgaaccgctt gaaatttata aattcttttg
      721 ataattacat aattatcagc tgcgcgctta tgagaataag gcacagaatt tttagccaaa
      781 agcgcagtag ttgacaatga agtaaacatg tcactttcat ttgaaaacgc gctcataacc
      841 gtaatattat ttaaatttaa ttttttatca aaaactgttt tttctgccat aacatatccc
      901 tcaagcttga tgatataaca tacaaaaata aaagtcatgc gtttttgtca aattattaag
      961 ataatttgac attttctttt ttgttataga atgttattaa taaaatatta caggaatcag
     1021 gaatgttttt atgaataaac cacaatcatt tgaagctatg ggagaattct tttcttcttc
     1081 aaaattttta aatctttgcg cattttttgg ttttgctttg ttgatgagta taattcttgc
     1141 atcacaaaat ttgctgtttc aaaacataat cgaaaacggg ataagcaaaa aggacataat
     1201 tgcccaaaga acaattaccg taattgacac aaaaagaaca gaacagcata aaagagaaat
     1261 ctctcaaaaa gttgaaccaa ttttagctcc tgcagaagac agttttatta aaaataacct
     1321 tgaaactctt gaaagctcaa tacttcaaat aagaaaaaaa gacgttccga tgtcagtaaa
     1381 aacaagcgaa ttatcaattc ttttcgatat ttccgaagga tacagaaaag agtttgttat
     1441 taactactta ttaaaaacaa acgaaaacag cgtaagacaa gtatttgaca aagcctctct
     1501 tgtattgaca aatatactaa gcgtaggaat aagtgaaaaa gattatgaaa acaataatat
     1561 aaggaaaata atccaccgta atatggtctc taacattcca aaaagtcaat caacggttat
     1621 tacagagctt ttggaacagg taattgtccc gaatttaata atagatgaag acgcaacaac
     1681 cacagccaag aaaaatgccc aagacgcagt caaacccgtt gaagtagttt ttgaaaaagg
     1741 cgataagata gtttttgaag gtgaacccgt aactcagctt aagcgtgatg ctttaaggaa
     1801 agctggttat aatgttctgg aaatgaactt taaaggattc ttaggaattt ttattattgt
     1861 tttattaact tctcttgcct ttttaaaata tttaagaaca tttgaaaaac aatatataga
     1921 atcaagcaga ataatgataa tgggagcatt atcactagca tttgctttaa tttctatctt
     1981 aatacctacg ggattttcac cttatatgtt accaatgcct gcatttataa taattctttc
     2041 aatatttaca actccgagaa tagcaatgat gcttgcatct cttatgttaa taactcttac
     2101 gcttacactg gagtataata tacaatttgt tcttgcattt gcattaatta atttaatctc
     2161 aatgattaca atagctcaag taaagttttc aagaaggttc gatttaatta aaacaggtat
     2221 agacattgcc ctgacatctg tcgtaatagt ggtaagcata tatttacttg aaaaatgcct
     2281 tattgaagtt gacaacgcat taatagtaac cgacacagcc ataacattta taaactgtct
     2341 tctcagcgga gtaatcgcac ttggactttt gcctttgctg gagagtattt ttaaaataat
     2401 aaccccctac ggacttgcag aacttgccga tcataaccaa ccgcttatga aaagacttca
     2461 atttgaagcc cccggaacat accaccacag ccttatggta tcaaatctgt gtgaagcagc
     2521 cgccgaatcc gtcggagcaa acccgatttt agcaagagtc ggagccttct accacgatat
     2581 aggaaaatta aaaaggccgt tgttttttgt agaaaatcaa tcttacttcg gcatcgaaaa
     2641 tccgcatgca aagcttaatc ccagattgag caaaatggta ataaccgcgc accccaaaga
     2701 cggtgtagaa ttagcaaaag aatacggact tccgccaatc atttataatt tcatcttgca
     2761 gcaccacgga gaaggtcttg caagctattt ttacacgcaa gccgttgccg aagaaggcgc
     2821 agaaaacgta aaagaagaac aattcagata cacaggccca aaaccaaaca tgaaagaaac
     2881 ggcgattctt atgatagcag atgcggttga atctgcgaca agggcactta aaaaccctac
     2941 tcctgaggaa atcgaaacga ttattgataa aattattata gaaagattaa acgacggaca
     3001 gctttctgac agtccgctta ctcttcggga tataaaaaca atcgctgcta cttttaacag
     3061 aattttaaga ggcatgcaac ataacaggat taagtatcac gaagatattg tccatgaact
     3121 ggataaaact aaaatcaata ttccgatgcc ggataaagtt atcgacaaag agcttgatga
     3181 aaaaataagg cagcttgaac aaggcggaaa tgaaaaaaat gataaaaagt aatatttttt
     3241 cagaaaatat tcatccgtct attaaaatta acgaaaaata ttttatttcg gctgcaaaaa
     3301 atattcttaa atattatctt caagacaata atatttttga caaatcctgc cttgcaaaga
     3361 aaaaatttaa aactttatct tttgatattc tttattgtga cagcaaaaaa gctcacgaaa
     3421 taaataaaga atacagaaaa aaagattacc ctgccgacat aattactttt gctatttttg
     3481 ccgattccaa cgaaaaattt gtatttgata atgaaataaa tttaggcgaa ataataattt
     3541 cgttggataa tgcaacacaa gaagcacaaa aaaaaggtac gccgttaaag gaagaattaa
     3601 agtttttaat cagtcatgga ataatgcacc ttctcggatt tgaccacgaa accaatgaga
     3661 agtataattt tattgtaaca gcacaggaca atgcgctaaa gaatttaaag ggataatatg
     3721 tcgaaatata agtcaaaagg gggattttca ggaacttgcc aaaatgcttt caagggcata
     3781 aggttggcac tcaaaagcga aagaaatatc cgtgtgcatt tttctatcgc gcttttggtt
     3841 accgctttgg gcataatttt aaaattttcg cctcttgaat tcagtatttt gctttttacg
     3901 atttcgcttg taattattgc tgaaatggta aattcagcaa tagaattttc actcgattcg
     3961 atatataaaa ataaatattc aaaaatggtg ggaatggcaa aagacatagc agcaggtgca
     4021 gttatgattg caactttcat aagtattgcc ataggaattt tgttgtttgg aagcaaatta
     4081 tttaatttat tttaaaatta aactttcaaa ctatatttgc tcaaaaattt tgccctaaat
     4141 cttgtaatcg gcagtgaatg tcttgcaaaa gcatcaaatt ttgtatcagg attgatttta
     4201 accctatgat caatataatt aattttacct tgaatttttc cgccaatagt gcttctgtca
     4261 taaagataat atttaggtgc aactatattt ttaataccgg ctgtatcatt cacatttaaa
     4321 ccttcttgtt gtacccaaat atcagaggct tgaccattat catgaagtgt caatcctctt
     4381 ttcacttcag atttttgaag ctttgcccag tccaaaacgt ttgcacaatc accaaccttt
     4441 acattaacag caacagaatc ccctctcaat gataagtcgc cattaatcaa agaattttca
     4501 acccttgcac tatcaataac ctttaatgta ttagttttca acttggtcgc aaaagcattt
     4561 tggcctatta aagaagattt ttttacagta acatcggaaa cagaagcaga attacctgca
     4621 acaagatcac cttttgaaat tgaacttttt actattgatg aatgtcccgc tccaatatgt
     4681 cgaagcgcaa ctaccttttg taatattgaa ttattatgag cataaaaaga gcctgcaact
     4741 gaatgagaga ctctcgattt attacaagta tttaaaacat gttcgacacc tgacattgat
     4801 aaaacagccg aatcatgtaa atctgcatta ttttgtattt ttaatcttaa agcccttgaa
     4861 ttttcccaag ccgaaaagtt atttttaaca acaacattat cggcaaaaga tgcattatct
     4921 aaatacaaat cacctacggt tgaatttctt acttttgatt ttccataaag atcaaggtct
     4981 tcagatacgc tcattttttt aagatttgaa gaattataaa attctgcatt ttttacttca
     5041 acgtacgaat ctaatttact gtttcttgcc tttaaatcat ctgcaaagat aaatcttgac
     5101 ttaaaatcac ctaaaatact tacaaattta gccctaatgc cgacattttg tccgccttcg
     5161 attttaattt tatcaacttc gataacttta tcaagacttc cgcctctttg aaaaataaaa
     5221 gaagcatctt taagtatttc ttcatcaaaa tattcatttc cgtcaagtat agtaatactt
     5281 ttgggcttaa gacccactaa atcgcctgct tcattcaatg cgccattact ttttaaaaag
     5341 ctttttatgt caccggaaag tttaccgact ttctgttcca ttaattgcag aatttcttgt
     5401 cccatttttt cagaatactt atgatactta atcagcaaac tgcccgtacc ttcaaatgaa
     5461 acattagaat gactctcttt gtcaacaaac atataatatc cctttctctt aaaaaattaa
     5521 cgatttataa aaaaatcaaa atatttattt ttgttatttt cttaataaaa atttacttat
     5581 taaattagac caaacttgct tcaagtatct gagcaccgtt ttcttctact ttgagagttt
     5641 tgatatctgt ttttacgttt aaatctgccc aagtttcgct tacgatggat ttaattttct
     5701 ccaaattgtt tttctgtgaa attattaaaa tagaaggtcc cgcaccgctt aggacacaac
     5761 ctaaaacgtt ttcttcgtgt ttaagatttt caatgatttc acaaagtccg ggaactaatt
     5821 tcatcctgta ttgctgatga agcttgtctt gaagagccag tttcattaac tccgcatctt
     5881 ttgaatgaat tgcttcaaca aacatcgcaa gccttcttgc gttaaaagtt gcatcttgca
     5941 ttggaacttc tttaggcaaa acagagcgtg cgatttcagt tgcaagttca aaatcgggga
     6001 tacaaacagt tatatgccat tcttcgggcc atttaagctt tctgtaaagg atacttccgt
     6061 cttcttcctg cgaagttaaa acaaggccgc cgactattgc aggtgtaacg ttatcgggat
     6121 gcccttcgac ttctgttgcg atagaaagca aagccgcctc atctgcaggt tttcccaata
     6181 attcgtttgc cgcgagaagt ccgcccacaa ttactgcagc cgagcttcca agccctcttg
     6241 ctatgggtat ttgagatttt attgttattt ttaattctgc aggattttgt ccgattgaat
     6301 tataaagcaa ttcaactgcc ttataaacaa tgttattctc atctgtagga atgtgctcca
     6361 ttgcaaaatc ttcttcggta tcaccttccg acattacatt aatttctatt ccggtgccgg
     6421 gcaaaactgt ttcttcaata gttacagtgt tataaatagg caatgccatg cccaaacaat
     6481 caaaacctga acctatattt gccgttgtag ccggcacttt aacgcttatt ttcatatttc
     6541 ctctgtttct tgtaatgaca taatatcaca aaaaattttt ttcaaacaat gccaattcgc
     6601 gcttatagtt tgatatacca tacatataga gggtatttca aaaataaatt taagatttta
     6661 aaaattaaag ccgatataac atatatatta acaaaatgag gtgtgcgcat gtcaacagtt
     6721 gaatatcctg caatgattta caaaaacagt cggaacaata attttaatgc aaattgtatt
     6781 gtaaaaaatg ttataggttt cggtaagacc gaagaagagg ctcttaataa tctcaaagcc
     6841 tctctgcaaa aagatacaaa cattgagata attataaaac ctatgtacgg gctttcgata
     6901 gcacaataaa ttattgccct attcattaaa aatatgctat tataaaaggg tgggagaata
     6961 atagtgcaat gattgacgaa agttgcaata atgctttatc agaaattaaa agtcgtgcca
     7021 agcacgtaat tgattcaact tccttgtaca aatacaaagg aagtgtttca aagcttttgg
     7081 gtttaacggt tgaagtaaaa ttaccgggct taaaaatagg tgatttgtgc tatatcgaaa
     7141 atatgcatgg cgaaaaaaaa gccgcagagg tcgttgcctt caagggtgat gttgcacagc
     7201 ttcttttatt atatgacgga gcaggaatcg gtcagggaag tcttgttcaa agcacgggag
     7261 gacctataat gcttcctgta ggagaatttc tgttgggaag attgataaac cctttgggtg
     7321 aacccataga cgggatgcca ttagacacaa ccgaagcaaa atgggtatca gttgaaaatt
     7381 ctccaccgga tgcttttaaa agacctttga ttaaagatat gttttcaaca ggagtaagag
     7441 ctatcgactc aacactgact tttggctgtg ggcaaagaat ggggcttttt gcaggctcag
     7501 gggtaggtaa aagtacactt ctcgggatga tggcaagaaa ttctgacgcc gatgtaaacg
     7561 ttatggcact tatcggagaa cgcggaagag aagtcagaga ttttattgaa aattcactgg
     7621 gcgaagaagg tatgaagaaa tctgttttag tttgctcaac aggtgaccag cctcctctta
     7681 ttcgtcaaaa atgtctgctt tcggctactg ccgtttgtga atatttcagg gatcagggca
     7741 aaaaagtttt cttaatgacc gatacagtaa cgcgctgcgc catggcggga agagaagtcg
     7801 ggctttctat cggggagccg cctacaatga agggttatcc gccttcgatt ttctcatggc
     7861 ttcaaaaagc actggaaaga acgggaaaca gccctgaagg gtcaatcact gcactttata
     7921 ccgttttaat ggaaggtgat gacatcacag accctgtcgt tgacacagta agagggattg
     7981 ttgacggaca cgttttttta tcaagaaaag ttgcggaaat gaatcattat cctgcgatag
     8041 atattttagg aagtatttca aggctagcta cagaaatcac aacagaagaa cagcaggaag
     8101 ctgcggcaaa aatgcgaaaa atccttgcaa tctacagaga aaacaaagat ttgattgatg
     8161 taggtatgta tcagcccggt actaacccga ctcttgacgt tgcaatagaa atgatgccaa
     8221 aagtaaatgc ttttttgcaa caaaaaactt ctgagagcgt aaatatggaa aacaccattc
     8281 aaaccttgat ttccatgatg tcaaatgttg aaatatagtt taattaagta agttttaaat
     8341 aaaatgttat tttaatatat tgttatgtac ccgacaaata attttttttc tttcggtact
     8401 ttttgatggc aaaaagtacc gaaagaaaaa tataaaaaca aataaataat atatcgcaat
     8461 gacgagaaaa tatagtcttt ttaaactatt aattattaag ctgatttgaa gtataatttt
     8521 tcttgaagga taaaacatat gctgcatttt gcacaaactg caatacatta tattaacagt
     8581 tcaaatttca gttactatga aattttaatg cttgtttgtt tcggaataag ctggcctgtg
     8641 gctttgctta aatcctatca gacaaaatca gtcaaaggca aaagttttct ttttttactt
     8701 ttaattttgt tggggtatat tttcggaact gtccataaaa tcatgtacag tttcgattat
     8761 gtaatctggc tttatatcat caatggtgca atggtcttag ccgatatggc tctttggatt
     8821 ttatacagga ataacgattg aggtgtcatg aaaaaaattc tattaataat tttatcagta
     8881 attatatttt cgcctaaagt atttgccgaa tctgaaaaca tagcactttt gacccaaaaa
     8941 gggttttttg acaagattaa aacgacttca caaaatcctt atgaagaaat tagagatacc
     9001 ttttaccttc acacaaaatt ttccaacgaa tacaaatttg agcaactaaa agatttatat
     9061 gccccaaatt attataatgc agacggtttc aacagagaaa actactttga cttgattaaa
     9121 aaaacctggg aaaattatcc cggaataaaa tataaaatgg aaattataga tataaaaata
     9181 agcggcaatt acgcgactgt atatcttgaa gaaagtgctc gtgcagatac agcttctgcc
     9241 ggcgggattt taagagataa aggctacctg caaagtaatt ccgacacgat ttattttttg
     9301 gaaaaaattg caaacgaatg gaaaataact tcagataata ttattttcga aaaaacatat
     9361 cttacctacg gaagcgcgag ttattgcagc tttaacctga atgctccttc aaaaattcct
     9421 atgaatacag aatatactgc gacattgaac ataaaatctc cgcctgacac aatagtgctt
     9481 gcctcaatcg gcagagaaaa agtaacttac cctcaaattc aagcagaaga agtctttaga
     9541 cgtttgccgg aaagcggcat tttagaaaga atgtttattt caaataatga aaacctaaat
     9601 gaatttgcgg ttgcttcttt tggtgtggca aaactcgatt taagcagagg ctcagacatc
     9661 aaaataaaca taaccggcat gggatttgca atgtcgcgag taaatgtgat ttcggcaaag
     9721 cttgaaaata ataaaaatga caaaaaaaag gattccgaaa atgacaaaaa atagtaaata
     9781 tacctatttt ttaatttcat ttctgttttt ttcatttatt gatttttact tttcaaactt
     9841 gattctgaaa aaaatcgcag aaagcggaaa tttcttaaaa tacggaatta attttattta
     9901 tacaaaaaac tacggtgcag ctttcagcat tatgcaaaat tcgagattat ttttaatcat
     9961 aatctctttt atagcattaa tattaatgtg ttattacatt gtaaaaaata ttgaaaatat
    10021 tttaataaaa gagctgtttt ttataacgat tttgatggcg ggaatttttg gaaatttata
    10081 cgaaagagtt gtgttcggtt atgttcggga tttttttgac ttaactttta taaattttcc
    10141 gatatttaat atttccgata ttttcataaa cattggagtt tttggtataa tagttatgat
    10201 aattttaact aaaaaaccga ttaaattgct atgaaagaac tctattttac cgttgaagaa
    10261 agcgatgagg gcaaaagact tgatgttttt ttgtcaaacg tttttgataa aatttcgcgt
    10321 gccaaaattc aagaattgat aaacaacaac aaagtatcgg taaatcaaaa cttcaagaaa
    10381 aactcatata aattaaagat aaatgataaa atttttgttc aatttgaaga acaagaacaa
    10441 gagataatca tttgccctca agatattgca cttgatattg tttatgaaga tgaaaatatg
    10501 cttgtagtaa ataaaccgtc gggcatgctt acccacccga cttcaacgga aaaagaaaac
    10561 acccttgtaa atgcgctttt atttaaatac ggacaaaatt tatctgacat taacggagaa
    10621 ttcaggcgcg gaattttgca tcggcttgac agaaacacat ctggcttact tatggtggca
    10681 aaaaacaata aagcacacga atttttagca aatcagataa aagaaaaaac cgccctaaga
    10741 aaatatttga caatagcgca aggaaatttt aatacagata ccggaataat taacctgccg
    10801 acttcaagac acccgaaatt ccctcacaaa agggctgtcg tagaagaggg taaaccaagc
    10861 ataactgaat ttagcataat agaaagattt aaagggtaca cttatcttga attaacctta
    10921 aagacaggaa gaacacacca aataagagtg cacttaagcc acattaaaca cccgattata
    10981 aatgattcat tgtacggggc tgcaccattt gcggttaaaa ctcaagaaca agttttacaa
    11041 gcatataaac taatatttac aaaaccattt tcctgtgaaa taattgaact tgaaattgaa
    11101 cccgatgaaa aaatcaaaaa agttttaggt tatttaagga gcaaaaagtt atgaaaatat
    11161 ttctaagaac aatagtagta attttatttg ttttatcatt aatttctttt tataattata
    11221 aatttgatta taattcgctt gaaatattat taatcggaat gtttataaca tacctttggt
    11281 atgttccgta ccgccgtgaa atatctaaaa aaattaaaga atatcaaaat aggcttgaaa
    11341 aaacttcagt aagtgctgaa accgacagtt caaaagtaga agttttggaa gcaaaaattc
    11401 aatcactgga aaaagcactt caatcagcgt tggaaaaaaa taaagactaa acaattacag
    11461 ctgctaatct gattttttat atccattaaa ttaagaaatg tatcattttt tattttttct
    11521 agcgaatttt ttttattttg tatttttata taaagtgaaa aggttaaaaa aaataaatga
    11581 aaattaattt aacaccaaac tgtatctcaa aaaaaataaa taacagaagt attttcacaa
    11641 ataatgcttc ttcatcacaa tttcgcaata agatttcatt taaaaatctt gaagaattcg
    11701 aaaatgataa atgggaagat agagatacaa ataacgatta cagtgttgtt tacaaatcgg
    11761 cagaagagac aaaaaaatat attgaaatta aaaaaaataa atacggctat atagatatac
    11821 ttttaactct attaaatcaa aataatcaag gagaaaatgt ccttcatcaa acaagcaaat
    11881 ggtctgctga taataataaa attaatgaca atgttaaaaa aacaagtata atcttaaacg
    11941 cgcttaagcc tgcagataaa attaaaattc ttgcatcaaa aaattatatg ggtgaaacac
    12001 ctatagatgt tgcgatagaa agttttggct atccttctga tatattagaa atgtttattg
    12061 aagaaatagc taacgcttta gaaaaagaca gctggcctga tataaattca aaaacagact
    12121 tcagagccat tggaaaaaaa ataattgaag attttcctga cgaaacaaat tttgaacaat
    12181 gcaaaaagct tgagagtcaa ttaaaaaaat aaataaatca aaaaacttac ttatttaaac
    12241 ttcttttttc tccacaccga cacttatttc ccaaccgtca gcgtttattt ctgattgagg
    12301 ttgattttca atttgaactt tttcaaaatc aatttcgtct tcttgaaaag ctgcagagtc
    12361 ttcaatttct tcgtcttgat gaggcaaagg tctatgttcc tgttcatctt cttggctaat
    12421 aggattaacg ggtaatctaa aaccgaaagt ggaaccttca ctaagctttg actgaacaaa
    12481 aacctgtccc tgatggtgtt tttcaatggc aattttaacc aaatgaagcc ctaatccagt
    12541 accttttata gtatgggtat cattttcaat tctgaaaaat ctgtcaaaaa ttttgcccaa
    12601 atgttcaggc gcaatgccct gtccctgatc ttcaatgctt atttcaacaa aatcacctac
    12661 ccgagctctt tctgctctta ttttaattct tccgttttcc ggagaatatt ttatggcgtt
    12721 agacaaaaga tttttcaaca cgcgctcaat gctgtcttca ttaacgggaa tttcaggcaa
    12781 atcaggctct ttcataatcg agaatgtaag atttttttcc tgcgccaaaa cctgcattga
    12841 attaaccgca ttttcaatta actgaacaat atttgtaact gttttttcaa gtttaacatt
    12901 agcagattca agccttgaaa agtccagaat atcatttaca aggcggttaa gacggataat
    12961 ttcctgattc ataaccccga taaattcctt ttgggtattg aaatcaaaat cgcttccgaa
    13021 attgtataaa gtatcaatat agctccgcaa aacggtaacg ggagttctta attcgtgaga
    13081 aacatttgaa ataaagtgtc ctcttatttt atccatttca acttcttttg taacatcaat
    13141 aagaacaatt atgtaaccta cataatcctt atttctggaa aacatcgggg aaataattgc
    13201 cttgataacc cttttatcaa cttcaatgtt gaaagccaaa ggctttttct ccatgacatc
    13261 aagcggagta tccttaaatt gctcgatttt ttctttaaag caaagttccc cgtttgaatc
    13321 gcaataaagc tgaattttac tgtttaaaat atctttttca tcaacttcca aaagcttttt
    13381 agcctgagaa ttcactaaaa cgactttgtc ataattgtca caaaccacaa ctccgtttgc
    13441 aatgctcatc aaaacagctt ccattttgtt gcgctccagt gtaagctgtt cgacattttg
    13501 ctcttcgtat aaatgcagac ggcttgacat gtcattaaac gcatcgaaca attcctggac
    13561 ttctgaactg acgccttttc cctgaacttt atagccgaat tcaccggtgg aaatttttct
    13621 tacgccgtgg tgtaaaattc ttaattcacg agtaataaga taagtattaa caagaattac
    13681 gactgcaaaa accgcccatg caacaacaaa aacaaacaac aaagatgctc ttgtagttga
    13741 agaaatttgg ttaataatat tacctgaaag ccctaccata acagaaccta tcgaagtaga
    13801 ttgccctaag cttttcataa ccataggaga acttaccgcg actttggaat cctgagcctg
    13861 tttaggaaaa tcttctttgc ttgaataaat cacattacct ttgttgtcgg caaattcaat
    13921 aaaagcaata tcgtcattac tgtttaatat tgactgcgta tgatacttta aagtactgta
    13981 tttggcatat tcgggaatat ccttaagcaa ttcaacgctt tcaatcgcaa gcgtttttga
    14041 aataattttg ccgaaatttt gatacccttc gtttaacttt gattggatgt ttaaactggc
    14101 caaaaccgca atcaaaataa ttaaaagggt acttaacacc agccccgtca agattaaacg
    14161 attttgagta gtcatactaa ctttattcat aacagaatta taccattatt ttctagcgag
    14221 agcaaataat tctcgctgca tgtacgcctg atgccgaagc ctgaatcaaa cctcttgtaa
    14281 cccctgctcc gtcaccgatt gtgaaaaggt ttttaacccc ttcggtttca agctcttttg
    14341 tcaactttac ccttgcagag tagaatttga cttcaacccc gtaaagcaga gtatatctgg
    14401 aagccgtacc cggtgcgatt ttatcaagcg catagagcat ttcgataatg ctcaacagct
    14461 gcctgtaagg aagcaccaaa cttaaatccc cgggtgttgc agacgacaaa gtaggagtta
    14521 ttgtacttgc ggcaattcgc tccggcgttg agcgtcttcc gtcaagcagg tcgccgaatc
    14581 tttgaacaag aacccctcct gctagcatat ttgcaagtct tgcgacgtgc tgaccgtaag
    14641 caataggctc tttaaaaggc tcggtgaatt tttcagatac caaaagcgag aagtttgtat
    14701 ttgcggtttt tcttgtagca tagctatgtc cgtttacggt caaaattccg ttataattct
    14761 cgcttacgac ttccccgtaa gggttcatac agaaagttct gacctcatcg ccgaaagtag
    14821 gtgagtgata aatcaatttt gattcatata atttttctgt aatgggttca aatacaggtg
    14881 cgggaagctc gaccctgaca ccgatatcaa cggcattgtt aaccataccg attttatttt
    14941 ctgcaaattc tgaagttagc caatcggcac cttctctgcc gggtgccata acgatatatt
    15001 gagcggtaat tttttctccg ccttttgttg taaacccttt tacttgttca ttttcaacga
    15061 ttaatttgtc aaccgcatca tcatataaaa tagtaatttt atcagtcaaa aagtcttgca
    15121 tatttttcag aacatgtaaa ctacgctccg ttcccaagtg cctcaccgga gaatgaataa
    15181 gtttaagttc tgcaagtgta gctttatgtg cgatatcggc aaaatcttct ttattagtac
    15241 cataaactac ttccgttgca ccaaaatcca aataaatttt atccgtgtaa tcgattaatt
    15301 tttcaacttc gcctctttcc atataatcga gaagatttcc tcccacatcg ggagtaagag
    15361 ttaatttacc gtcagaaaag gcacctgcgc caccccaacc gcataaaagc ccgcattttt
    15421 tacagcccgg acaagactta tgaccttcgc gaagaaagca ttttctcttt tcgattttct
    15481 tgcctttttc aaccaaaacc actttccagt caggtttcag tttcattatt tccaacgcag
    15541 taaaaatccc cgcaggacca gcaccgatta tcgcaacgtt atacatctaa ttctccaata
    15601 gtctattacc tacatatttt agtagaaaag atttttaatt aaaaggtttt attaataagc
    15661 gttaagcctg ttttttaata ttctcaaatc ctatattctc aagattaagc ttaaagtttg
    15721 atttgccgta ctcaccgcta cttgtcataa aaacatttaa tctcatagga tcgccaatca
    15781 tcggaccatt gcctgtttct ttaacttctt ttaatcttaa tttaggattt tttatatatt
    15841 ctaagatttt atcaaaataa ctgtttcctc ttgcgcttgc ttcaaatgtc tttgatgttc
    15901 cgtaagcatc caaacctttt gattttgcgg ggcctatttt tcttgctaaa gcatcgatat
    15961 tccaagcttc actacccgta aataagtatg catcaacctc ttttttgaaa atataatttc
    16021 tcaaacagcg acctggttct tctacccctg gaagaaattt aaaatctttt acagcattta
    16081 taaaaatttt tataatattt tttgactgtt ctttaccctt tgaaccatgc actaaaatat
    16141 tagacaaagc cttaatagct ttttgaatat ttttcctgtc aacagaagga agcccgtcta
    16201 tatagacctt aacaggaatt acacttgtaa aattcggatt atttctttga acggaattaa
    16261 cttgcatgaa aacacactcc ttgacttatc cttattaaaa tccgtaatta aaaatatttc
    16321 acttttttaa ggggcaattt aacaaaaatt aacagtaatt atttaaaaag aaaaattaac
    16381 aacccgtatg gcttaagtct tgaatctatt cttgcattcg gatttgcctg aattaacctg
    16441 tttaaaacct ttcccgcctg cgcaaaatta aattctctca aataaatttt tgcaagatta
    16501 atcgaatcaa caattttttc agggtgcttt gaaagcgtgt cgatataagc ctgctttgca
    16561 aaatctttat tacctttatt aaaattaatt gctgcaataa gcccgtctat atcagagccc
    16621 gattcgggcg acattccttt tgccatatcg gcatatttta aggcttcatt ccaatcctga
    16681 atattcatat aaatcaccgc atagttataa taaacaacat aacgttcgga atcatttgaa
    16741 gacaaagtca aagctttatt cagggcatct atagccaaat tatattcttt catgcctgca
    16801 taaaccgaag caagctgaat ataaccgttt ggtaactccg gtgctgaatt tatgtaattt
    16861 tcggcttcca taatgcctct tgaacttatt tgctggtcag agcccattac aataggagga
    16921 taaataaatc cttttgtaat taacttgttt ggcgcggtgt ttgttatctc aggcgcaagt
    16981 ctgtacaaaa gctgaagcgt atctaaatca accggcgaaa taagctgaaa gacgcttctg
    17041 tgtctgtcat aagttttatc cagattattt tccataaaca ttaagttgtc tttgttgtag
    17101 ctgtgcccca taataccgag cgcatgaccg atttcatgca aagaaacgtt ataaacctct
    17161 cttggcgtaa aatgttgcgc aagattgttt gaatcgtaaa aaataatatc cattctttta
    17221 agaaaatctc cgcttataac cggcgtggta taagctacaa catatttaca gtcttctttg
    17281 gaacacatgc tcatagcatc aagaggaaca atttttacgg tgatttgggc ttcattttta
    17341 ttctgaaccg ttttaaattt aattaacccc gctgttgaag actgccactg agaaaaagcc
    17401 gatgaaatct gctcgatata atattgcgga agatttttta cggtattttc aatgtaaaca
    17461 tttaaaggaa attttgttgt gtcccacctt aaaatcctgt tgttgaaagg cacttgttca
    17521 atgtaatttt ctccaatatt aaaataagcc tgttttctta tattagaaac ttctttatct
    17581 gcaataaggc ttgcagaatc atccgattta aaatttgcaa aatcatacaa ttctttttgc
    17641 atttcaatag tcggttcaag tttaattaaa gattgaacat aatagtagcg taaatccctg
    17701 tttttgttat taaaatcaag tgcgattttc agtatattgt gcgcaccggt ataatttttg
    17761 ctttcataaa aacttttccc gatacttaaa aaaacagcgg gtacaatgct tttgaaagaa
    17821 actataataa gcaaaagtga aattaaactg acggcgataa ttcttttcaa ataattattc
    17881 ctcttcgttg tcttttacac ctttatcaat ctctaaaatt ttgacaagat tttttgcatc
    17941 acctttaatt ttaaaatact ctgcaaattt ttgagtggtt tttaaattaa aagaacgccc
    18001 gtttttatct ttattccttg aaatcaaacc tttatcgaca agctcgtgca catgttcata
    18061 agcgttagaa cctcgcaaat ccttcaaaaa ggtctgtctt ataggctctt tcagagcaat
    18121 aacagtcaaa gtcttaagca cagcaggctt taattcaacg ggacaaagct tttctactat
    18181 atccatgtgt tcggatttga cctgaagaat atagccttct tcgtcatcaa tctccaatgc
    18241 cccgtcacgg gatgaatagt ccataattaa atccaaaagg gcttcttctg tctcttcaaa
    18301 tgaagattcc agaatttcgg caatttcttt aacttttaaa gcctttgccg tggtaaacaa
    18361 taccgcttct attcttgatt ttaagccttc tgtcatttat ttttcaacca tttacttctt
    18421 gttacgtaca aatcagaata aaattctttt tgaacaagtt cataatcgct ttctgccgac
    18481 aaaaacaaaa gtgcaatgta agctgtaact ttatccattc caagcagcgt aagctcatta
    18541 agttcaattc ttttttcttt cttaaaaatt tgttccaaat tttctctcaa tttttgaaca
    18601 ctgtcttcga tatattcatc atgtgcaaga ttaacaatat catcgggcga aagatttgca
    18661 aaagagcgaa cccttctttt tgcacgctca tgagcgtttt ttaaagactg ttttctatca
    18721 atcatttcgt aaaattctaa ctgacggatt aaatctttta aggttacgac acggtttctg
    18781 tttaacctta cgcttgttct gcgctgcaaa acttcgtcta tagaaataac atttgagccg
    18841 taatcttcat aaccgttatc aaaatcatcg ttgtaatcaa gatcattggc ttcttcttcg
    18901 tagcttccct caaattcaac tatatcgata ttttcaagga catttgattt taatttaagc
    18961 aaaatagcgg caaacaaaag tgttctgccg gtaagcttaa ggttttgggc tttcatttga
    19021 aaaagctggg caagatattt gtctgtaata tcaacgatat caatgttcca aggattgacc
    19081 ttaccggttt ttgccattga aacaagaatt tcaatcccgt ccatttcttg agaaacagga
    19141 ttaagcgcaa cgaattcaaa attaacatcc gtctgccctt ttaaggaagt atttacgtaa
    19201 ttataatcaa tcgcttcggt catctgtccc ttttacccgt cactctctta actttacacc
    19261 tgtaactctg gtaatccctt tttccttctg agtaacaccg attgttctat tagctgattc
    19321 tatcataggt ttacgtaaac taactactat aaattgcgta ttttccgcct gatgtttaat
    19381 catgtccgat aatttttcaa cgttaattcc gtcaaggtgc atatctactt cgtcaaaagc
    19441 gtaaaaaggt gcgggcatgt atttttggat agcaaacaca aacgctaatg ctgtcaaaga
    19501 tttttcaccg cctgacatac cttctaatct ctgctttttc ttatcgcgcg gctgtgcttc
    19561 tatcgtgagc ccacctgaaa gaggatcctc ttcattttcc aaaatcaaag ttccttcacc
    19621 ttcagatagc ttatgaaata cctctttaaa gtattcgttt atactgttaa aggtcttcat
    19681 gaaagtttct tttttaagat tttcatagcc ttgcatccgc tctaaaatct gttttctttc
    19741 tttggagagg gtttcaatct gttcttttaa ttcctgttgg cgagcaagca catcgtcata
    19801 agccgtaagc gcgcgcatgt ttacaagtcc caaatcttcc atacgctttt caagccgctg
    19861 aatttttgcg gtaatctcct cgattgaaat cgtaacaggc tcaagcttgt tgacttcaag
    19921 ccctgatgat tcaagttctt ctcttgcaag ttcaagctga ggctcaagtt ctctgcggcg
    19981 agccttaaag gattctatct gctctgcaat cctttccaag tctgacacgt gaaggtgctt
    20041 ttgagtttcc atttcaagca gttccgaatt aatctggtca cgttgcgcca aaagttcacc
    20101 gagtttttcg ttaatttgtt caatctgctt ttcgtaatca tcaagtttat cctgaagcac
    20161 ctttatttct tcggcataag tctttttgtc ctgctcaagg gtgattgaac tttgttcgat
    20221 gtttttaatt tcttcttctt tggtttttat caaatcctta aagaaagcaa tttcgcgacc
    20281 catttcggac aaatcattgt ttgcgttggt taaattggtg ttaagacgct taatttcaaa
    20341 ttcggtactt tctgtttttt ctttcaaatt tttcaaatct tcttcgttaa taagtttttc
    20401 aactctttcg atttcacttt gaagttcttg aatagaagaa gttacctcaa catgagaaga
    20461 ttccatttta tcaagttttt ttgaaatctg ttcaatttgc ggctctgtaa gttttatgaa
    20521 ttcaagtttt tctgcgatta aattttcgtt atcttcagaa tttttgacaa ggtttgcaac
    20581 ttcaatgtta gccttgttgt actctgtcat tgtatttgaa taatcgcttc tgacactgtc
    20641 gagtttcttt tcaagttcga tttttttatt ttcgagcgat gcgagatttt tttccatttc
    20701 cttaagtttt gccctgaatt tatccaactc ttcatcatcg ttctggctga acttaagccc
    20761 tgtttttctg acagagcctc ctgtcataga acctgatttc tcgacaattt ctccgtcaag
    20821 ggttacaact ctgaatctgc ccataagctt tttggcgcaa tcaaggttat caacaacaag
    20881 agtatcgcca agcgcgtaga aaaatgcgtt tgtatattta tcttcaaagt caattaagtt
    20941 aatggcataa tcaataaccc ctttgtcttt aggcaaaggc agctttgaag gagcttttga
    21001 aatcttgttc agcggaataa aagtcgcgcg tcccgcatcc gaagatttca aaagctcaat
    21061 cgccactgag gcgacgtgtt catcgtcaac cacaacatgt gtaattctac caccgattgc
    21121 aatttcaagt gcggtggaat attttttatc aactttcccg agcttaacca aaggtgcgtg
    21181 aacccctttg agtttagcgt tcataacagt atctattgct ctgccgaaat tagcatcttc
    21241 aaccgcctgc ttttgggctt ccattgaaga aattttgcga taagccattt gaatatcata
    21301 attcaagtcg gaaatttcat ttttggtagt atccaaatca tgcatggcgt tttgctgaac
    21361 gatttttaaa tcttccattt cacgctttag ctgttcggat tgagttgata tcaaatcttt
    21421 atttgagccg aaattctttt taaaatcttt taatttttca acctgttcct gcgcttcacc
    21481 gagttctttt tgaaggtttt ttaattcatt ttcaagcggc aatttttctt taattaaatt
    21541 attttcttta tcctgcaagg cttcaaggtc tttgcgaagc tggtttcttt tttcgatatg
    21601 ctggtctgcg gtctggttta agccggtcat ttcttcaaga attttattta actcggcttt
    21661 ttgaagcgcg atattttctt cgattccttt tatttcatct tttttaagct gaattttaag
    21721 ttctgtatct gcaatttttt tgttttggac ttcaattccg tttttggcgt tttcaatgga
    21781 tttaagcccg tcgtgaattt gtttgtccgc ataatttatt gccgactgtt ttctttctat
    21841 tgcgcctttt gtttcttcgg caagtttttt tatttcaatc tgctgggctt cgcctttttc
    21901 tttaacaagt tcgcaaatct cctgatattt accttttata agttctaaac gctcttccaa
    21961 atctttgtgc ttaacttctt cttctttttt ctttttggtg aattcaagaa tattttcgtg
    22021 tgctttttca aggtttcgtt ttatatcaaa gaattttact ttattaatct gtgattcaag
    22081 atttattttt tcgtctttaa gtttttgata ctttagcgca acttctcttt cttccttcaa
    22141 ctgctcaagc ctgatatcga cttcgctcaa aatcagggaa gatttttcca cccgtttttc
    22201 aactgtttca agctcatttg tcgcctgttc tatcctgcgg tcaaaatctg cgactcctgc
    22261 aatttcgtca atgatttttc tgcgctcatt tgaagaacag tttgtaatgc ttgttacatc
    22321 gccctgcata ataacgttat aactgtttgg ggtgatattg tgtttttcca aaatagaatg
    22381 aatttcgctc agggtaacaa ctttatcatc aaggtaataa gtgctgatat agccttgcga
    22441 ggatttttta attttacgcc cgacaacaag gtcttttccg ccttcgactc cgccaaaggt
    22501 aacttttacc agcgcgtcgt ttctttttgt gtgggttgaa ataagatgaa aaagtttttc
    22561 tgcacgcaga gttcttgaag tggaaagccc caatgcaaat aaaaccgcat caataatgtt
    22621 actttttcca gaaccgttag ggcctgaaat ggttgtaaaa cctttgagta aaggtatatc
    22681 cattttattc gcaaatgatt taaaattgtc tatttctaac tgttttatat acatctattc
    22741 tcacacccaa gtctaatcgc ggcaataagt cattcctgtg actttttcac ggttcacagc
    22801 tcacacttca ccacttctat tacactacaa acaagcagtc atgtcaaaat gttaagctgt
    22861 attttaataa tttttctttt tcattttttt atttaagact tcggaaaaag aaattatggg
    22921 tacaagtttg tagccgcagt tttcgcttaa ctccccgccc atggcaaaaa gttcttcctg
    22981 cgggtatctt ctgcaaattc cggggcggtc tttgtgaatt ttacaaagat tagttttttc
    23041 gtccaaattg gagcaggaga aaacaagccc tgtatcatct ttttcaacaa catttaaata
    23101 agtgtaaaac ctgtgaagat attttaattt ttgaaactct ttttcatcct gaataacatg
    23161 gtttgcatgc tgaacataaa ttttcttgca gcatttaccg cagcccaaac attgccctat
    23221 tctgtagtat ttttttttca gaactttcgt ataaaaaaaa ttctttaaat tttttaaaaa
    23281 atttctaatc accaaacggc tccgtctcag ctttttcgca ctgtgaatat ttggcttttg
    23341 cttttgaaaa ttcaagaaac tgaggggacg aaagtttcat tattcgccaa aaatatggtt
    23401 ttgcctctga gaacctacct tgattgacca aatcatttcc tatattaaaa caactttcaa
    23461 tgattgaagc ctcgggattt aagtaagact cgagtttttt aattaaatct ttgtactctg
    23521 cggcactttc gggctttaaa atagaataat ttttaaactc gtcaatagca tcaaaaaact
    23581 cgctttttgc aatatgttct tcagcctttt taagatgctc ttcaattaaa tgctgatttt
    23641 cattcaaata aggctttaac ttcaaaagtc ttttgtttgc cttgtaatac tcttctttat
    23701 cacctattac aaaattcata taacggtaat aataacagta ggcataatta aattgtttaa
    23761 acaaatcgta agaaatggca agatttgagt aaatttttgc atagttaggg tccagatgcg
    23821 aagctacttt ccagtattta attgcaagtt caaggttttt gtctgcaaaa aatatacttc
    23881 caagattaag gtttgtttta gaaagattag gataattagc aagcgattta aaataacagg
    23941 cagcggcttt tttttcattg ccctgactgt gaagttgcgc acctgcggtt aagtattggt
    24001 ttgcaacgtt ataacaatga gtaattttac ttttaacttt tgcatattca gaagaagact
    24061 tatcttcagc atatttaaga tatttctcaa aatacttaat ggattcaaaa cgcatcattt
    24121 tttgattgaa cgcaatagca aggtttaaac acgtatttgc atcctcaggt cgagaaatca
    24181 aagcaactag ccaataagaa atagccattt cgacctgacc gcctttatag taaagatttc
    24241 ccaaatttgc ataagcttta gaacattttg aatcaagttc aatggctctt gtccaatatt
    24301 caatcgcctc tttttcatcg tgaagcatat aatgacaatt acccacatca acataaacat
    24361 ctgcacttgc attagtattc aaaatacccg taaaaagcct gagtgcctca ctgtaatttt
    24421 tgttcaaata ctgcctttga gccgcttcaa gaacaccgcc ctcaatatca ccgttatttt
    24481 gaacctgata ttcagctata ttttgttgtt cttccatacc ctaaccgctt tattaaaatc
    24541 aagaaaataa atcttttaaa agtctttcat tagaagaaag tttttccata gcctcgtctg
    24601 ataaaggatc ttcgaccccg ccggcaaaaa gcttagaaat aattgcatca tgcgaaaaat
    24661 cattcggatc agacattgcc agtttggtaa aagtccttat atcctgctct ttttcataca
    24721 tagatacagc ttcattagat attgctgtct catcaattaa aaaaccctta tcaatgctca
    24781 tataagcatt tttaatagga tttgtatgca gcttgctaac ctcagatttt ttaccaatct
    24841 gggaatttaa aatatcttct gcgtttttaa cctcgtttaa catttttccc caatacttta
    24901 actacataag tattatgcac tatttcagag catgctgtta tcaaccctca ggtctaaatt
    24961 aagatagacc atgtgattaa tattataact tattaatgca ggtttgccaa ttagttatat
    25021 ttcttataga ataagtgaat aagtgattaa gtgactaagt gattaagtga ctaagaaaga
    25081 gaaaataacc atgcttgata taaaattaat cagagagaat cccgaaaaaa caaacgaact
    25141 tttaaaaaga agaaaccccg aactttcgat agatgaagta cttcaaatag atgcccaaag
    25201 aagagcggtg caaacacaag cagatgaaat gagggcaaaa agaaaaaaag actctctgat
    25261 aatcggtgaa atgaaaaaaa aaggtgaaaa caccgaccgg cttcaagaag atgtaagaaa
    25321 aatcggcgat gaaataaaac ttttggaaga aaaagaagtc gaacttgacc aaaggcaaag
    25381 aatattgctt ttaaatattc ccaatacccc cgacgaaact accccgaaag gaacttccga
    25441 agctgacaat aaaattgttt caggctgcgg agaacctaca aaagctgatt ttgaattaaa
    25501 agcccattgg gatatagcag aagaaaaagg gctggtagat tttgaaagag cggtaaaaat
    25561 ttctcaatca agatttgtaa tgaacagggg caaaggcgca caattagagc gtgccataat
    25621 aaactttttc cttgatgttc acacgaaaga acacggctat gaagaaattc tgccgccggt
    25681 tctggttaat tccgctgcaa tgacaggaac aggacaatta cctaaattcg ctgaagatat
    25741 gtacaaatgc gaaaatgagg atttatacct tattcccaca gccgaagttc ctgttacaaa
    25801 catttatcaa aacgaaattt tggaagaaga agatttgccc aaatacatga cagcctacac
    25861 tccttgcttc aggagagaag caggctctgc cggcaaagac acaagaggga tgataagagt
    25921 tcaccaattt aacaaggttg agctggtaaa gctcacaacc cccgaacaaa gtcatgaaga
    25981 acatcttaaa cttacaaaag acgcagaaaa agttttggaa atgcttgaac ttccttacag
    26041 aaggattgaa ctttgtaccg ctgacatcgg attttcggct caaaaatgct ttgatttgga
    26101 agtttatatg ccctcatata acggctacaa agagatttcc agttgctcaa attttggcga
    26161 ctttcaggca agaagagcaa acataagata tcgtgaaaaa tcaagcggca aggttcaatt
    26221 tgtccacacc ataaacggct ccgggcttgc cgtaggcaga acttttgcag ctattataga
    26281 aaattttcag caagaagacg gcacaatatt aataccaaaa gcacttcacc cctatacagg
    26341 gtttgaaatc atatgatgaa taaatttccg tttgaattag acgaatttca aaaagaagcc
    26401 tgtaaatata taagcgacgg caaaagtgtt gtggtttgtg caccgacagg agcaggaaaa
    26461 accgtaatag cagaacacgc aattcatctt gcgattgaac aaaactgcag agttttttac
    26521 acaactccgc taaaagcact ttcaaaccaa aaatacagcg atttttcaaa tcaatacgga
    26581 ataaataacg tcgggctttt gacaggcgac acttcaataa accgagatgc tcaaattgtg
    26641 gttatgacaa cagaagtctt ccgaaacatg ctatacggca caaatttcgg ctctgttaca
    26701 gaaaacatga aaaacgtaaa atacgttgta ctcgacgaag ttcactacat gaacgatgag
    26761 caacgcggaa cagtttggga agagagtata atttactgtc cgacaaatat tcaaattatt
    26821 gcgctttctg caactgtcgc aaatgctgac caactaacag actggataaa taccgttcat
    26881 tcgcgcacag agcttataaa cacagatttc aggccggtgc ctttgagatt ttattatttc
    26941 gacagctctc agccaaacac tattttgcca cttctgacac cgagcaggca gcttaacagc
    27001 aaaatccgcc ccgaaaaaag gcaatttcat cacagaggga aagtgcccga aagaaacaca
    27061 gtccgtgacg ttgtaagaaa tttacacgaa aaagacatgc ttcctgcgat ttactttaca
    27121 ttttcgcgaa aaaaatgtga cgaccaaatg gaaaaatgtt ccagaatggt tcttgtaacc
    27181 ccgcaggaac aaaaacagat taaagaaata attgacgaat atttaattga aaatccttat
    27241 ctgtgtaaaa acaaacatat cgaatatgtt ttaaacggcg tagcctcgca ccatgcaggg
    27301 cttttgccgg gctggaaagt cttaatcgaa aacttattta aaaaaggact tatcaaagtg
    27361 gtgtttgcaa cagaaacgtt ggctgctgga attaatatgc cggcacgctc aactgttata
    27421 agctctataa gtaaaaggac agacacggga cacagaacgc ttaccgcaag cgaatttctg
    27481 caaatgtcgg ggcgcgcagg aaggcgtggg atggatgaaa taggttatgt tacagttgtg
    27541 ggttctcagt ttgactctcc acaagatgta gcggaattgg ttctatctga tgctaatccg
    27601 cttgagagta gattttcacc gggttactca atggtcttaa atcttctgca aagattttcg
    27661 cttgatgaag caaaagaact tatcctgaaa agtttcggat atttttcttc cacatcaaga
    27721 cttacgccgc ttttacatca aaaagaaatt aatcaaaaag ccatagatga gctttcaggc
    27781 tttgtctgcc attacaaatt aaccaatcag gatttgctcg attacaacaa aataaggtca
    27841 atttatgttg aaaacagaag aattttcaaa acaatccaaa aacaagaacg ccacaaaaat
    27901 cgccccttaa atgctgaagc gcaggaattc ggcagaaaaa acaaagaaat gctctataaa
    27961 atgcagtctt acaaatgcga cacctgcaaa cttttcaaaa aacattcaaa agaaattgaa
    28021 gttttagagc gatacaaaat caaacaaaca aagttagaaa aagacattga acattcaaaa
    28081 gatatttttt ggaacaaatt tgtatctcac aggcaagttt taaaggattt cggatattta
    28141 aaagacgatt acccgaccga aaaaggggta atggcttctc aaataagaag cgaaaacgaa
    28201 ctttttatag cagaaatcat tctttcgcat gtgcttgaag cactcgaccc tgcagagctt
    28261 gcaagtgtta tttgtgctgt tacgacagaa gatttgagag cagaaattta cccgcagatt
    28321 ccgatttcac aggcggtgag aaaaacgctt aatcagatta aaaacataaa aagaaatata
    28381 gacaaagccc aaaaagacaa taatatagaa accccgatgt acataaattc ttactattcg
    28441 tcaatgatag aaatgtgggt taacggagcc gaatgggaaa gtattcttga ccaggtagag
    28501 atgggagaag gcgatgttgt aaggattttt aaacgtacgg ttgacgtttt aagacagctc
    28561 tgcgtaattt caaatatttc cgaagccctt gctttcaccg caagagaggc aataaacgga
    28621 atattaagag agcccattga tgtagattag aaataatttt tatactctaa gccggctcta
    28681 tatcaactgc cgctgtagat agttcttctg tcgttgtatc atcaacaata ttgtcctgct
    28741 taagctctct ttgttttcta tagccgtaaa ccgcataaat taaaaacccg accaaaagcc
    28801 acaatgggaa aattgtagca gaagttttga gattcttcat cgaataaatc atcaatccgc
    28861 cgcaggttat aatcccaagc caagggaaaa gcggagaaaa aggtacccta aaaggtctgt
    28921 gcctatcagg atctgttttt cttaaaatta aaaccgcagc acagacaatt ataaaagatg
    28981 tgaaagtccc aaaattgcag agttctgctg atatgtttaa gtccataaac aatgcgccga
    29041 taatgaccac aatgcccgaa acccacgtta aaacgtgcgg agtatgaaat ttcttatgaa
    29101 ttttattcat acttttaggc aaaaatccgt ctctggtcat cgcataaaga attctagttg
    29161 ttccaagctg ataaaccaag agcacggatg taagccccgc caaagcccca accgaaataa
    29221 atccggcaag tctgtcttgt cctacaagcc tcattgcatg tgcaataggt gcttgaaaat
    29281 cgatttgatt ataatgaatc ataccggtca aaacaagcga aacaagaata tataaaacgg
    29341 tacagataac taaagttcct aaaataccaa taggaatatc tctttgaggg ttttttgttt
    29401 cctctgcggt tgtagaaatt gcgtcaaatc cgatatacgc aaagaatata agaaacgcgc
    29461 ccataaacac cccttcaaat ccgtttggag caaaaggagt ccaattttca ggttttatat
    29521 aaaaacttcc gaaaattata aatgcggtta taatagtcaa attaagcccc accataatgc
    29581 ttgcaagctt tgtagattct ttaatcccct taataagcat aagagttaaa agaaaaacaa
    29641 taaaaattgc aggaatattt attgcaatag gaattccaaa aaaatgagga ataacaacat
    29701 tagggtctaa accttctctg ttgcagatta ttgttgccgt acgatagtca ttaatgagcc
    29761 aaatcggcgg gcttacaagc caagccggta atacatttga aaaccccttc aaaagttgaa
    29821 acaaataacc cgtccaagca cttgcaacag taatgtttcc gatagcatat tcaagcatca
    29881 aaatccaacc aaccatccac gccataaatt cacccatagt cgcataagta taagtataag
    29941 cacttcctgc aacaggaatc attgaagcaa actccgaata acaaagtgcc gcaaaaacac
    30001 aggcaacagc tgcaataatc attgaaacaa ccaatgcagg acctgcaccc acaccttcag
    30061 gaccgccttg tgcggcaatg cccacaatcg taaaaatacc cgtaccaacg accgctccga
    30121 tacctattac aattaaatcg aagatgttta aagttttgct caactctgat tttgcacctt
    30181 cttcaattaa ttcatcgggg ctttttgttt taaacgcgtt ggtaaatata atttttaatt
    30241 tagagattaa cgggttcttc atttatttta atttcacttt cattttctgt aatattttta
    30301 aattcctgtt ttctattcat tttatagccg taatggaaat aaatcaaagc acccagcccc
    30361 acccaaacag ggaaaagcaa agatgacgtt gttaaaaatt gcatagaata aatcatcaaa
    30421 ccgccgcaac aaattatgcc taaaataggc aggacaggag aaaacggaac tttaaaaggt
    30481 ctatgtctgt caggatcggt ttttctcaat atcaaaatag caacacaaac aattataaag
    30541 gaagtgaaag ttccgaagtt acaaagttca gccgcaacat ttaaatcaag agtaagtgtt
    30601 ccgaaaatgc aaattatacc tacaatccag gtcaaaacgt gcggagtatg aaatttcggg
    30661 tgcaattttt gaagcctttt tggtaaaaaa tgatctctgc tcatagcgta caaaatccta
    30721 gttgaagcca tctgcaacac caccaaaacc gaagtgagtc ctgcaagcga acctattgaa
    30781 ataagccctg ccgcccagtc ctgccctgca atcgacatcg cataagccat aggtgctttc
    30841 aaaaatcctg caggaattac cccgccgctg gtaggctgca tgccggtaag cacaattgca
    30901 accaaaacat aaactatggt tgtaatgacc aatgagccta tgatcccgat cggcaaatcc
    30961 ttttgagggt ttttacattc ttctgcagta gttgaaagag catcaaatcc tatatatgca
    31021 aagaaaatta taaaagcacc cataaacacc ccttcagcac cgtttggagc aaaaggagcc
    31081 cagttttcag gtcttacgaa aaaagcacca accaaaacaa aaagtgcaat caccgcaagc
    31141 ttaataaaaa ccataattcc cgccattttt gtagagtctt ttgttccttt gacaagaatt
    31201 aaagtaatag caataacaat taaaatcgcc ggaatgttaa tacttaaagg aattcctgca
    31261 atcataggca taaccgtgtc gggattaacg cccgaatctg ttaaaatttt agccgcagag
    31321 cgcgggtcgt taacaagcca aacaggagga ttaacaagcc aatacggaag ataattggaa
    31381 aaccctctta aaaattgcat aaaatgattt gaccatgcgc aagcaactgc gatataaccg
    31441 attgcatatt caagcatcaa aatccaaccg accatccaag ctgcaaattc accgagcgtt
    31501 gcaaaagtat aagtgtaagc accgcctgca acaggtatca tcgcggcaaa ttcggaataa
    31561 caaagtgccg aaaacacgca cgcaacagct gcaacaacca ttgaaaccac aagcgcaggt
    31621 cctgcgccga tactttccgg accgcctgct gctgcaatac ctacaacggc aaaaattccc
    31681 gagccgataa ttgcgccaat acctaaaatt attaaatccc aaacccctaa ggttttgtca
    31741 agtccctcat tttttgcatc attaagcata tcatcggggt tttttgttct aacaatattt
    31801 tgcaaaaatt ctttagaaaa tcttgctctg tgaaggtgtt ttgtcattta tttatttcca
    31861 actttttcat atttgcacaa aggaaatccc aatttttccc tctgctctac gtataatttt
    31921 gcaactgcag cagccattgt tctcactctg tttatgaaat ttattctttc atctttgctt
    31981 ataactcctc ttgcatcaag caaattaaat gtatgcgaac acttgagaac ataatcataa
    32041 gcgggcaaaa ccaattcttt gctaacacaa ttttcaacct ctttttggta taagtcaaac
    32101 attttaaaaa gtgaatcggc gcaagaaact tcaaagttat atttggactg ctcaatctcg
    32161 ttttgaagat aaatttctcc gtatttaagc tcgttgttcc acttaacatc aaaaacagaa
    32221 tctacatttt gaaggtacat agcaattctt tcaagtccgt aagtcaattc aagtgctaca
    32281 gggcgaattt caagcccgcc gacttgttga aaataagtaa attgagtgat ttccattccg
    32341 tcaagccaaa cttcccagcc cacgccggca gctccgagag taggtgattc ccagttgtct
    32401 tcaacgaacc gcacatcgtg tttactcaag tcaattccca tagcttctaa actttttaaa
    32461 taaagctcct gagcatcgtc gggcgagggt tttaagataa cctgatactg aaaataatgt
    32521 cccaatctgt tagggttttc gccgtatctg gcatcagtcg gccttctgca aggctcaacc
    32581 atggcagtat tccacggctc agggcccaaa gagcgcaaaa aagtagcagg attcattgtc
    32641 gaagcacctt tttcaatgtc gtaaggctgt tgaattatac aaccgtaatc tgaccagaat
    32701 tttgaaagat ttaaaatcaa ttcctgaaaa tttaatgctt tttctgccat aatattccca
    32761 actagcgtaa caaatacact ctttttctgc cgcttaaata ttatagcggt aacaaatcaa
    32821 aattgcaata aaacaggcat aaatattaac ttatttgaca gagtttaaac atttgaatta
    32881 tgattaaaaa gaggtaaata tggcagataa aattataatt ttttcaggca aacaatattc
    32941 gggcaaagac acagttgcaa aaattatgat gcaggaaatg ccagaatata aacgttttgc
    33001 aatgggtgat gtcataaaac ttgaatacgg acagaaaaaa ggactttctt acgaagaaat
    33061 cgaaaaaaat aaaccgcttt acagacagga tttgatagat ttgggaaatt ggggacgctc
    33121 acagcatccc gattattggc ttaaaaaaat catctcacag ccgggcaaaa ttatggtaac
    33181 tgatgtcaga gtccctcacg aattggaaat cttcagggca gcaggtgcaa tcgcaataag
    33241 agtggaagct tcaaggcaag taagagcttc ccgcggtgaa ttaataggcg aaaacgatgt
    33301 tacagaagtc ggactggacc acataaccga ctggggtttt gtaatcgaaa ataattcaac
    33361 ttacgaaaaa ctgaaagaac aaacttttga agtgattaaa caattatctg cctgctaaaa
    33421 atccgaaaac ctttttaaaa tcaattttaa aataagataa aatgatttgt aagacaagtc
    33481 cgtttgtgcc gcgtgtgcct atagctttat taaatgattt tttattttta aagataaaat
    33541 tcaaagtgtc acgtcttatt tttctgttgt tgtcaaggtt taattttgtt acctcgtcgc
    33601 agtgataacg cttttcgccg caaacctgat ttgaggcacc ggcgttattc ttatgtctgc
    33661 gataacccac caaaggtgcg taaacaattg cagaaccgcc aagtaaatgt gcaaaattca
    33721 gcaaaaattt atcaggacaa attctccaat tttggggtgt gtcgtaattt aaaaataaat
    33781 caagtgccga ttttctgaac atatttgcgc tcattgcaga ccaataccag ccgccgaaag
    33841 gctttttctt aagcgtaaaa acttcaaaat ccacattatc aacatcgatt ttaagtaaat
    33901 ctttcagcga tttaagttca aaactttttt ccttttgaaa agatgaaacc gagtaagtcg
    33961 tgtgaatttc ttcatgctcg ccgatttcta cgatttgagc cgaaacaaaa gcgacacttg
    34021 ttgacaagtg aactctcaaa agcgttttgg cataatcttt tattaaaatg tcatctgaat
    34081 caacaaagca aataaactgt ccctgagtat tcttaagccc tgtctgaatt gcagccattt
    34141 gacctttgtt acaatcatgt tcgataattt ttattggaac acgtttattt ttttgcgcaa
    34201 attcttttat cttctcgacg gatttatctt tagaacaatc atccacaaca attatttcaa
    34261 tattttcata agtctgattt ttaatgcttt cgagcgtttt aacgacgtaa tcttcgtagt
    34321 taaaagaagt tacgacaaaa gaaactagcg gtaattttaa taaatcagtc aacgttttcc
    34381 ctcaattttt tattatggat taaagatgaa ataaatgcac ccgcaataaa gattagccct
    34441 atagaccctg ttaaaatctc attgacctta taaaaagtag aaacaagcat gattaaggac
    34501 agagccccga ttgcccaatg agcaccgtgt tccaaataca aatatttgcc taaggttttc
    34561 ttttcgacaa gcatcatcgt aagcgacctt acaaacatcg ctccgattga caatcctatt
    34621 gtaattatta aaatatcctt actgagagca aaagcaccca aaacaccgtc taatgaaaaa
    34681 gaagcgtcaa ttaattccaa atacaaaaat cccattaagc cgctcccttt aacggtttga
    34741 gcaataagtt tcgtactttc ttcttctttt tgttcaagcc attgagtaat tccgtctatt
    34801 aaaagataaa tgaccacgcc gaaaatacct gcaaggacaa catacagtct ttcattttga
    34861 acaacataat gctgggttaa aaacaaaaaa atcaaagtca caattgtttc agcaccctta
    34921 atgtttccca tttctttaaa tcgtttttca atccacttaa gccaatgaag ctctttttca
    34981 tcgttgataa aataatcaag aaataacatc atcaaaaaag ttccgccgaa agaaacaatc
    35041 ggcgcatggg tcaaatgcag gtaatgttcg tacattttag aatcagtcag tgctatttta
    35101 acgacttcaa aaagtcccaa atgagcaaaa acagcaacta caagcacggg aaacaaaaat
    35161 ctcatcccga aaaccgcaat cgcaatcccc caggttaaaa atcttcgacg ccatttttcg
    35221 ttcatttttt caagtttcat ggcatttaca acagcattat caaaggacaa actcacttcc
    35281 aaaacgctaa gtacaaaagc tataaaaaca gttgtcaagc cgcttccctg atgtacatgt
    35341 tctccccaaa aatatgccaa aacaagccct acaatagtta ccaaataaga actaaaaaaa
    35401 taattcattt aaacctcatt caaactttcc tctctaatta tataacattt tggctttaaa
    35461 tcaatttatt aaaaaataac tccgtcgggc agtggtattt taaaaatttt ccacttaaac
    35521 tacacatagc taatttgtta tccaagatgt ccccaaacac tatggagcac gaaaatgaat
    35581 agtcgcgcaa aagtctgtga ctttatgtca catgaaaatt ctgtcaaaaa tcttctcaaa
    35641 aaaggattta ataaagtttt tacctatcct caaagtgacg ccagagaagt tgcaaaaatt
    35701 atggcagata taaacacaga ttctctgccc gttctgctaa gcccatggag taaaaaactt
    35761 gtcggaatca ttgaattaag aaaaattaaa aaattcttag acgattaaga tttttagcca
    35821 aaagataaaa aacccctaca atgccggtgg aaattaaaag tcccctaaag tttttggcat
    35881 cgctatggcg tttcaattta ttttggcttt caaaaacatc tttatgcaat tctttaaatt
    35941 tttttcttgc ctctttatca ctataaattt ccggattagg tatatcattg ggcggcacaa
    36001 taacacccac attgggtttt gcaactttta taacatcact tttatctacc gtagtcataa
    36061 tttaataaaa acaaaaagca aaaagaaatt gcttaaatat gaatcaaaaa cctgaaataa
    36121 atataaattg aacttaaaat aagcgaaaca aatgtaatta atgccccata tttaaaaaat
    36181 tccataaaag aaatcggatg acctgcttta gcagaggttt ccaccacaat aacgtttgcg
    36241 gctgcgccaa taat
//
LOCUS       DAOQAU010000012        33755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1208_length_33755_cov_8.796291, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000012 DAOQAU010000000
VERSION     DAOQAU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 33755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33755
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_33755_cov_8.796291"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3384)
                     /locus_tag="PKI94_03345"
     CDS             complement(<1..3384)
                     /locus_tag="PKI94_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25805.1"
                     /translation="MADKNLENIEYKFESINNLLEAMRAQNAANAGDVDKVLANINLK
                     LDTISDEENTDLIKVFLAELKRSLEERHNFVSSKFVEMEEAYKQLVEKAGESVKAQDI
                     KQVFDIIATNLNVFSKEVFTQKELLNQINTKMEESKLDDTAKKDILKNISLLKVELEK
                     FNNGFESIILNLGSNFQTMSESLLKLDQSEALADVKKDIENIFLSSNAVLSTLQVIDR
                     KNRELEDVLTQVVTKEDFKIEQDQVAKLVAQNIQLSDYISKLPVNEVVEDLNKKIDTT
                     VGVINALKNILSEATDQNQKMLSAQIENLESKIANISSEEEFIGFRKELSDFAAEVMQ
                     SSNLMRADLLDTNSELKKLTAFINSSNLKNDFEEIANTVKASENNLAQNISEASSNFI
                     QSALNNKSELEQNIIQSTRQITEKIDATEKTVSENSKSNLTNILDGIQSVISNIFSAK
                     NEINLSNIENSENVEIKIEELQKDLNASKSALIQNSKKNLEEINTNLQAVINFTNETR
                     TLLEGNVAQNSKLVEEKLEELSKKLTEAQNFIDKNSKESTEKISALNEELIAGLNAFK
                     TESQEGATTTAAWVKQTITELTRELEDTKESLETKSKLNFEGLLAKIQGISQEIKDSK
                     EGLETKSSTNFENLNKNFDEISKEIQGVKENLEEKSNLNFEGLSEKIQEISHSIEQAR
                     NLSEEALGEKISKLKDEVSILPLTIKENQQSFTQESKILIEENSKEIENLSTKIQNLI
                     GGLQSKDKPFQEELMEEISGIIKKLEAAKENLSSENAQNLETLTQNFTQIQDGLKISE
                     EKMSNLSTELGRKIDGLLLNLTQMKELSAVKEDENFQKTLEIIDGEFAEMAEKLEELK
                     TSQKSFCEENFINLSQKISEVNQLVSNISEVNFDKSFEDLKTEIQAINTEFIKFSEKI
                     EESPQKVQAAMGIFGDKLLDKIDSLGADLGKNQKDTREKILEKLEDFNPIEKISELKS
                     DLIQNQEDIKASLFERFDENIDVSKDNLDEMISLKNGLLEEIDVMKKDLKDMLKEENE
                     GHITKLKADLQELIDEITKAQEEIASHKLDLSEILKVQDEEAKTQIAELENTIVDEIA
                     HNVEEFKTSVQQTVSQIEEKIAASAQNYNSSLNSLVTEIK"
     gene            3631..3849
                     /locus_tag="PKI94_03350"
     CDS             3631..3849
                     /locus_tag="PKI94_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW25806.1"
                     /translation="MSGIKIDKTKCKACYLCVEVCPKKLIMIGSEANSLGHFPVEFVD
                     EKNECIACAMCAMRCPDMAITEVVNRKY"
     gene            3902..4951
                     /gene="vorB"
                     /locus_tag="PKI94_03355"
     CDS             3902..4951
                     /gene="vorB"
                     /locus_tag="PKI94_03355"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNW25807.1"
                     /translation="MKQLIKGNYAIAQAAVNAGCDCYFGYPITPQSEIGEYMSKKMPE
                     LKRGYVSAESELAAINMVLGACSTGVKAMTSSSSCAVALMQEALSYIASDELPAVLIS
                     VMRAGPGLGYIYPSQADYNQATIGGGNGDYKIITLAPSTVQECVDLTYKAFFLAHKYR
                     NPVILLADGLLGQMMAPVEIGKYPYPEISNKDWALEGAKNRQSRAIFSLEPLEENLIK
                     RIDKLFKKYKKIEENEVEFEEYQTQDAELIVVCFGSITRNVKSAVKECRKQGKKVGIL
                     RPITLWPFPSKKIEELANKTKKFLVVEMNMGQMLKDVKLAVNGQVRVEFQGKSAGQLI
                     GVEEIVSKIERLIND"
     gene            4944..5705
                     /locus_tag="PKI94_03360"
     CDS             4944..5705
                     /locus_tag="PKI94_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNW25808.1"
                     /translation="MIEVKENKVFEIPKSIKKGAKFPFCPGCDHGVAIKLIAEVMDEL
                     GLREKTIGVSSIGCSVMLYDFLDIDCVEAPHGRALAVATGVKRAKPDNFVLTYQGDGD
                     FASIGLAESMHAALRGEKITAICINNTTYGMTGGQGGPTTVMGQVTTTSPTGRNKEYH
                     GYPIKIAEQIALCDGTVYATRCSLDSIPNIRKTKQAIKKAFEIQLEGLGFAFVEILAT
                     CPTNWRMSPQKAHQRVRDELFQIYPLGEFKLVNSK"
     gene            5838..6392
                     /locus_tag="PKI94_03365"
     CDS             5838..6392
                     /locus_tag="PKI94_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNW25809.1"
                     /translation="MNENSIILAGFGGQGILLAGEVLATAFMLDGKNVTWFPSYGAEM
                     RGGTVNCEVVVSEDEVSSVSKKEADFIVALNQPSFDKFLPKIKKGGWIIANSSLINEE
                     KPRNDINYVFAPITNIANDLGEVKISNIISLGILAKVSNLTSIQSLHSALDSVLPEHR
                     KNLLPLNLKALEMGFNFASELVKC"
     gene            6477..6719
                     /locus_tag="PKI94_03370"
     CDS             6477..6719
                     /locus_tag="PKI94_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25810.1"
                     /translation="MNKKDVDDLKKTIQEKEVQLAKLKAHVDKSSICSDLYNKVVLEK
                     AILKKELQDVENNTLTSKIKKIIPRKKTLICDYFKK"
     gene            6986..8539
                     /gene="metG"
                     /locus_tag="PKI94_03375"
     CDS             6986..8539
                     /gene="metG"
                     /locus_tag="PKI94_03375"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNW25811.1"
                     /translation="MEKFYLTTAIDYVNGAPHIGHAYEKILTDVIARHFSQRCDDVYF
                     LTGTDEHGIKIQKTAAAKGISPQQMCDEHFAKFQQAWKVLEVNYSQIIRTTDDNHKKI
                     VQQIFKKLYEQGDIYKHSYQGLYCSGCESFLNEKDLIGGKCPVHNVEPELVSEENYFF
                     KLTKYKDAIIKHIKENPDFIVPAFRANEVLNQLENIEDISVSRSKENVSWGIDVLDDS
                     TQIIYVWIDALSNYITALGYDVEKPCDTFKKYWPADVQVIGKDILKFHAIYWPAILLA
                     LDLELPKHLFVHGFININQEKMSKSLGNVISPVEIMKNWELQTPDALRYYLATAAPFG
                     KDGNFSDEDFKEKVNADLANNMGNLLNRTLSMLVKYFEGGVNVLWNSDKCPIKPLETV
                     EKVKKLFDNFEIQEAANEIISLVDLTNKYVAENAPWSLAKEEKWDECGQVLTNVLEIM
                     CVVCVLIYPFCPNIAQDMANQLKFDLKTKLDDLNLNNIKQGKLIEKEDIKPVFLRLDS
                     EFATNKGKM"
     gene            8624..9661
                     /gene="prfB"
                     /locus_tag="PKI94_03380"
     CDS             8624..9661
                     /gene="prfB"
                     /locus_tag="PKI94_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129742721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNW25812.1"
                     /translation="MCKKHKTVFDPEKLKKELFEIEFKLQLPEIWADQNKASSLGAQV
                     REIKENLELTSRWQNILDDAEVAIELGEEDLVQETFENLSNLEKELDKYDIRQMLSGE
                     YDDADAILSINAGAGGTDAQDWAEMLLRMYSRWAEGKGWKVDLLDKSDGEEAGIKSAT
                     IKISGKFAYGYAKAEKGVHRLVRISPFNANGKRQTSFASCEVSPIIEDFEKKIVIPPD
                     DIEIETMRSGGAGGQNVNKVETAVRIYHKPTGIVVKCQQERSQLQNRENAMQVLASKL
                     LALKQAEHEKKLAELKGQNFDINFGSQIRSYVFHPYKMVKDHRTNFEVGNVDAVMDGD
                     LDGFIEIFLKS"
     gene            complement(9658..10482)
                     /locus_tag="PKI94_03385"
     CDS             complement(9658..10482)
                     /locus_tag="PKI94_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW25813.1"
                     /translation="MKTIVITGATSGLGNLLVKEYANMGCLIFAGYRNEKFIKELEQI
                     SQNVIPFYIDMSKKETVVNAAKFICEKTDKIDVLINAAGFVVAGAMECLNTDKIKEQF
                     EVNTFSHLEFTQGLFSKLQGARVINISSMASFGIFPFIAPYCASKRALDILFNSMQIE
                     CGHDIKVISIKPGVIKTPLWDKSIKNNQEDLGGNQKYQREFEFLAKNAQKNNKKGLEA
                     QKVAEIIVKISECKNPKASYTIGKDAFFAQLLSHLPQNWMNFLVKKGFEARLKKQP"
     gene            complement(10496..10774)
                     /locus_tag="PKI94_03390"
     CDS             complement(10496..10774)
                     /locus_tag="PKI94_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25814.1"
                     /translation="MTEENDFISGVWSEKVLVVTQDYEIKGYVFMPKTGKKNRILSDI
                     LNGGKRFVAIKDCEISYRFLTNRRTEYHNFLQLNLNSIVLLRPTTEDE"
     gene            complement(10789..12000)
                     /locus_tag="PKI94_03395"
     CDS             complement(10789..12000)
                     /locus_tag="PKI94_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNW25815.1"
                     /translation="MTVFSYVALKNNNDVVKGKIEASNIKEARDHIRKLNLIPTKVYE
                     ESQAKTVAKVDAKKIQTLGLQDKIDFTSTFQILTQSGIPVIEALVFMENDAAKLKIRL
                     VAKELRRQIMAGSTFADTIAKYPKIFGQIYIGLVRAGEDSGELEKTLDRLLELLKKEA
                     AIKSKVVGTLMYPAFVILLAMVVVTVMLVFVFPAFKDMFDNQGKPLPWITAALMSIGE
                     FLKTYWVLIPIIFGSVIFGVTFLLNWEPSKRKIDEFVLKVPILSDLIQFSNFSNFIAV
                     MQVAYDAGVPIVDCLYLSNLTISNYTLQDKVSQATTKVQQGQHLSLALRSTQVMPKMI
                     LFMIATGEQSGRLGEMLLQATAFIDKKLDAIIDLMTKMVEPIMLIVIGSIVLVLALAL
                     YLPLFSSYMTD"
     gene            complement(12012..14078)
                     /locus_tag="PKI94_03400"
     CDS             complement(12012..14078)
                     /locus_tag="PKI94_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNW25816.1"
                     /translation="MENVLEKLKDSINKDIVAKFNKTILENYQFLPINMKGECLYVAT
                     NPSAEKETIAKVVKSMGIVDVKFIQIAEVEVDALLKYVYGDDSGIKKLLEEAKNKTQG
                     QTQEQNPANGEEAPKKKIGEILIEKGYINEEQLQKALEEGKRLKMPLGSALFKLGFIT
                     LSQLKETLKDQQGYDMVDSDQLKVQEKVINMLPEDFVRANKVIPISSDGSTLVVGMVK
                     PDNKKVLKDIVFLTGQKPRAMLITHFEFQSFVETEYSATKKETNKIIKEIEEENLEFT
                     EEESLADQVERELQDTSGTVVKFVNKIITDAIDNKVSDIHIEPRLENYVVRYRKDGIL
                     RQIIEVPPRVEMSVITRFKVISRMNIAERRRPQDGTFSLKYKGSSYDFRINTLPVGGK
                     EKVCIRVLAPAVSLNSSDKEIHLMGGMPEDIEKIKKMTSCPNGIILTSGPTGSGKTTT
                     LYSVLKSLNDEEVNITTIEDPVEIKLEGLNQSQVNAKAGITFASCMRAILRQDPDIIL
                     VGEIRDYETLEVAISAALTGHLVLSTVHTNSAAATVTRLVEMGAKDYLVSSTLSGVIA
                     QRLVRKLCDDCKDGYFPSKEEASKILIDEGEIEKFTKTKVYRPIGCSNCDYTGFKGRL
                     GVYEIMPITKEIKKLIATGAHDIDIEEAAIACGMKTLNQACLSHIINGFTTIDEFVRV
                     LGLANE"
     gene            complement(14079..15893)
                     /locus_tag="PKI94_03405"
     CDS             complement(14079..15893)
                     /locus_tag="PKI94_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HNW25817.1"
                     /translation="MKITKILSCFMVSAFLMTYIAPIGFCAQEIQLRANKIAEKEPLP
                     TVLKLEGEISITKSNPKISLSLRDSDVKQVLRMFADKAGLNIVFHNSVDGKVTLDLVN
                     MPLNDAFKLVMQISDLTYYIDKNTMVVASASAAQSLNLSKQEMVAIPVKYVEASLLAE
                     FMNKNIFSINKPGLSNSQIAITNPAANEILIFGTDNDVKMAQKIIEKFDVKPSSETFA
                     VNHTTPKEMATLLCTVLFPPSGASSGGSAGGKATGGAADAASGELALGENVIACEYDN
                     KISAGNISSFNSKSMIISYFPQRGTISIKGGSQRQIEMIKEFIAQNDVRQPQAYLELS
                     IIELNESGMRDFSNTWQYLSKNFSANFDGTTKSNPYYPMFFRNDEYIVTDPMKTGDDA
                     TVQKILKYAGAPSLVYSINYLIQNQKGRVLANPRIMITNGQTSTVDLTADYVKTVKSE
                     VLANSGGMAGAVQKTYEIGSDEGIKVEMIPFISPDGYVTLNIKPEYSTVKEKIYTPNA
                     DDPTVKDLAVTLLQRRNLDLKNIRIKDGETLVLAGMIREDEQKSVSKIPVLGDLPGVG
                     FFFRNTNTTKSKQELVIMITPKIIKDNDELVQNEGATL"
     gene            complement(15911..16843)
                     /locus_tag="PKI94_03410"
     CDS             complement(15911..16843)
                     /locus_tag="PKI94_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25818.1"
                     /translation="MVSGLDNEEENIRYSEVGIKNVESVDNSAEEKKGFILVKNRNEG
                     IALVVVGILLVCALGYASFTQYSNNSQNVDTSASEALLAEAQQKGLQSLEQTDSAEQI
                     QQDAQDIYSKTLALKGEKAPDNMPSQSQKRVQIPVSQTSSPQTASNNDDVEIVSKKNA
                     GKLLNVDKRVLIQVSESGRQNPFVPTGELSMLSAPKFTLINPPDQLGTGSDADKVMGT
                     TISGILYDRYSPSAIINIGGNDYLVKKGDVINNYRVLGISKDQVVVQLGQNVYKAGVG
                     QLLVQSEINYNTIANLEKKFAGNDVSIKVKKKKK"
     gene            17061..18710
                     /locus_tag="PKI94_03415"
     CDS             17061..18710
                     /locus_tag="PKI94_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNW25819.1"
                     /translation="MKDISVIAPVKEPKDIEEFIPHTKCRDFYVYYDRFLNNNFEYIN
                     EFIEAAHKNSCKIYINFKHDISEEDLVEIKKMIRFLKTTKIDGIFINSFAVLEAIKVF
                     SLPFKIIIDSYFDIHNLSGIDFVNTFHKVDQIIITEEIYMKNIAKIKKYTNLPLAIDS
                     DNLPWCAEDIKKLKAIDSVVIKGKFGSPEEILEGIELVEKILDKPKMFKNQKLPFKHV
                     RNSFYRTDHFSGEMVSAEGRDFKFSRNIQKFDWEIKRTKLKKEAPYKKDPDFKINLRL
                     SGLEQLNQTKKFIQKLGYNPVDSIEYGEILSTSDLSKSSFNEIIVKVKKFCEAQGIKL
                     QLSTPRILIERDFDRVFEYVKNLCLTPPIPSSLIINNIGYFWAVINDNDLNRIPIEIG
                     QGINLLNSMSITCLNNLHPIDTVDFTGFSEGESTIHTIKKIKNLINNRKYTVAGNIRV
                     PSLGLCPLNNDSAIISRLSCKAPCHKGEYALKDPSLKKIYPFTVDGFCRMHMFEDKIM
                     QRFDQIEELKDAGINEFVFDFSALNAKFVPILLTNFLNSQK"
     gene            18785..19300
                     /locus_tag="PKI94_03420"
     CDS             18785..19300
                     /locus_tag="PKI94_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25820.1"
                     /translation="MKNKISLFLALTVAILGFGLALDVQAQPQKKKSVVVQKTVLQPS
                     APVYVDAKSLDIVANPTKYLNKRVKINAKFDKFSTLGLDYKPAFRSSEKYISFLIKRD
                     DVINHVVPLSEMKIFMKREVAEKHIDLEPGDIVEFDAYVFSNALGDPWLEVENFRVLS
                     SKAKDKKVNSK"
     gene            19469..21964
                     /locus_tag="PKI94_03425"
     CDS             19469..21964
                     /locus_tag="PKI94_03425"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNW25821.1"
                     /translation="MSHKEVFLAIHGHFYQPPRENPWLEAIELQDTAAPFHDWNERIN
                     NECYNPNSVSKIVDSRNRILDVVNNYSMISFNFGPTLLSWMENFAPLAYERVIKADIE
                     SVHQRDGHGNAIAQVYNHMIMPLASEQDKQTQVIWGIKDFEARFGRKPEGMWLAETAV
                     DDETLRVLAENDIKFTILSPYQALKVRRLGEKDWTDVSWGNIDPAMAYRYYIKSSPKK
                     YIDLFFYDGAISKSVAFDDVLKDGNKFIRRLKEGVSDHREYPQLINIATDGESYGHHT
                     KFGDMALSYVLRVKAKEEGFTLVNYSQYLAKYPSLYEVDIKQSSSWSCFHGIGRWKED
                     CGCSTGGHPGWNQKWRKPLRDALDYLRDELALIFEKEGKNYFKDSWDARNNYIDVILD
                     RSEMTVKKFQKENFKSELSEEQKVKAMELLEMQRQAMLMYTSCGWFFSEISGIETTQI
                     MKYAARAMQLAQNFSKKDLEKHFLELLDEAKSNFAEFGTGKDVFERFVKPSIVTTKQI
                     ASLWAVSSLYQEFDDEEDVYCYKIKKNSYKKVQKGTSQLVMGHIEIQSKITLQKSNVM
                     FALVQYSGGDFHCAIKEFSAESDYINTQKELFRIFMLNPLTEIIRAMDEYFGKEYFTL
                     KDIFIEERRKILEIMLKGKMNKFAQTYQEMYNEGKSSIYHMQSLGLSIPDEFKISAGY
                     TLSRQFNDLIVHSSGFLDSNIIQQAIDINYETKRIGINIDKKPTNQIFSQKITQNISR
                     LAQSLEIQQAEAILEIFDNVEKLELEVDIAEAQNIYFTKIYHEIGDIIENMTKTSKAE
                     DRNFILILLDIGQKLNINTEFYRSMLDKVLIKK"
     gene            21966..22736
                     /gene="mazG"
                     /locus_tag="PKI94_03430"
     CDS             21966..22736
                     /gene="mazG"
                     /locus_tag="PKI94_03430"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNW25822.1"
                     /translation="MYKNLEELIEIVRILRSPNGCQWDREQTHASLKPNMLEEAHEAI
                     DAINENDLKHLKEELGDVLLQIVLHSQIADEQKSFNIDDVAKGIKDKLIRRHPHVFGD
                     VKVSSTKEILDNWEEIKKEEKPHRNSVMDGVSKSQPALMSAQKISKKAVKVGFEWDCI
                     ETLYECVFSEFEEFKQACAVKNSEEMEEEMGDILFAVVNLARWNKIDAEQALLKANKK
                     FIKRFKQMEKLATKPLEEYSFEEFDALWKCAKKTNNYK"
     gene            22829..24151
                     /locus_tag="PKI94_03435"
     CDS             22829..24151
                     /locus_tag="PKI94_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNW25823.1"
                     /translation="MTNNKNPKEKYSVFLKHVEEEKEETSKFAKTLLVLVFLVCSLVV
                     VFLFLLDDQETVEQLFGRDNIVSQKLVEFVQSRSHKHRAEFSIPFLPHKQNILLLGVD
                     SNGSDTDPWEGTRSDTIVLLNVDPKTRSVNAISIPRDSKVYLAGNFGVQKINAAHALG
                     GIKLAKRTVEEALGVKVDRYIVVSDEAVRKIVDAIGGVPIYVEKNMFYNDYSGDLHVH
                     LNKGLNILDGRNAVGYLRFRHDGLGDIGRTQRQQWFLRGLLEKIQTPQAIMKLPEMIN
                     IASTYVKTDLSLYEMSQYAAMAKSFDVSKIEVATLPGAPNKKGYTSYWILDPDKTQEV
                     VNRLIYRDRPDISDKKFVAGIMYSPEKEQEAMEVKAKLEEMNFEVNCVGRGHLPHSQF
                     VANSSSVTSEFFNWVKRKIPAIRRSQFVYDPNKFYCVNSDFVIILSGQ"
     gene            24273..25262
                     /locus_tag="PKI94_03440"
     CDS             24273..25262
                     /locus_tag="PKI94_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW25824.1"
                     /translation="MARQCALKAEKPNNLFKSLQQKYIDETDWEQIEGEDLIQECKTF
                     NSISNEDDILQMYLKDVGRVKLLSGNEEKSLGKIIKEKRDIQADIAKRKLIQANLRLV
                     VSIAKKYIGQGVLFMDLVQEGSLGLIRAAEKYDYSKGFKFSTYATWWIKQTIIRAISN
                     YSRSIRIPVHMTDKIRRYKKTVSELNFVLGREATEKEIAEKMGLNVKKILSIKRSIIK
                     EPVSLDSPVTDDLNVEDYVQDGKSSSPEEKTSINALCDGMECILDELDEREKKILTYR
                     FGINGVQTKTLEQLGNLMGFSKERIRQLEDGAIKKLRQQENIRHFKDFITSEE"
     gene            complement(25326..26612)
                     /locus_tag="PKI94_03445"
     CDS             complement(25326..26612)
                     /locus_tag="PKI94_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25825.1"
                     /translation="MLKPSVKRNLMLDFVSDEFSCPENIDDARIFTLNDKIGSGAVVY
                     LMSRELRFQDNWGFIFAHNLAQKTGKNLKVLCNLDEKIYSKRQIEFNLPHVKNLSKIA
                     KKNDIDFEFIKDDLQKYLKIFGALVIDFNPVLKFDKAAKSFVTKDFAAQAIFEIDSHN
                     IIPARFISDKQEFSAATLRRKVYVNIGRFLTEFKNPFPVEKSEAQEKMFDFIENKLNY
                     YTELKNDPTQNFVSGLSPYLHSGQISAQRVALEVLKSNASRENKEAFLEELIVRKELS
                     DNFCLYSKDFKSLKSTPAWAKKTLEEHKFDIRPYVYDLKIFEDAKTHDELWNAAQKDL
                     VQNGKIHGYMRMYWAKKILEWSQSPEEALKTAIYLNDKYALDGNDANGYVGILWSIVG
                     VHDRAFSSRPVMGKIRYMSAQGCKNKFDVKRYIEKI"
     gene            complement(26637..27026)
                     /locus_tag="PKI94_03450"
     CDS             complement(26637..27026)
                     /locus_tag="PKI94_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25826.1"
                     /translation="MRINSVQNYYNQTVKSNMSFGKKTEQKNHVSPEYNKVLMQEAAG
                     IAALTLLISLLSLAPKNNNLQPQKKSVTELSQYIPQPELPQQPVEDNYQETKQSMQVY
                     RRERDENIMPEAEGENDLDTANLDIEG"
     gene            complement(27181..30291)
                     /locus_tag="PKI94_03455"
     CDS             complement(27181..30291)
                     /locus_tag="PKI94_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNW25827.1"
                     /translation="MLQEATRALNSAFNNSFIKKLSQYLHDCVREEVKSATFRNLKQD
                     KDNKWIFLKEEEFLFHNYGEYLRLDGSDSKLTELMIQSELSQKDKYLIYGYLFLVGKS
                     SKNKKQNEFLTPLLYMPCKLERNGVNINCMLQDEFLSLNTGALTAMMKKNDDEDEVEQ
                     LLEGLLDVVPELPITDEKLEIFLTTLKSIIPDIEIKIDHSEDVDEDNIEKDFYNEVEK
                     IDFENLEEAVEEGSVKTAVKLEKITLTNQCSIILTKRPSITAGVLHELMQIAEKPSGM
                     YRETALNIINEEYLQTKGKLVDNPIKQLKDLKDFVAVTPLSLSDSQENVIKSLENNHF
                     VAVYGPPGTGKSQTIVNLVAHLIANGKTILVASRMDKAVDVVSERLNELGAPFLALRA
                     GRLNYQKQLSFQLQDLLSNKVDLDSEYENAIFADVKDMKDLVNTLRDLENRCEQVIKL
                     EHEWQELFVQKNEQEKALGQTQFIKELLKKAEIEQVENLISTIEKNLEKTGFFVSLSN
                     YSSKNKLKKLLKLNDFNENYENLSRLAQELEFSKTACEMRLIEANIQKLGNLHVMAEQ
                     IRMLKRKQRNLAIDILKSKRRESLKGLLRDQIKRQRLIVHTKSLVERKKNLQNRLLEG
                     EDFKPLLEAFPCWCVTTYAISGSLPMKPGLFDVAIIDEASQCDIASCFPILYRAKRAV
                     VVGDDKQLPHLSFLEKAKEQSFLSQYSIPDKYQLMWRFRTNSMFDLANYYSMNPVLLD
                     EHFRSLPPIINFSNDEFYGSRIRIMKRNKNEKVLELVYVPDGKVDFDATRNLPEVEAV
                     VKKLQEIIIEDERKNPDNPTSIGIISPFRAQVEQLKTSVARILSEHMIRKHSIEIGTA
                     HTFQGDERDIMLISWAFASNSFVQSLTFLQKPNLFNVAITRARHKMINFISRDPKELP
                     DGLLRSYMSYIQEYQNKQQAIENKEIDENFYKNNFEREIAQAIRDLGHEVKAGVEIAG
                     FSLDLLVNDKIAIECDGVEDSKKLSGSNLKKQSILERSGYKINRITFREWQYSPKACL
                     DRVLIE"
     gene            30352..31563
                     /locus_tag="PKI94_03460"
     CDS             30352..31563
                     /locus_tag="PKI94_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25828.1"
                     /translation="MDIINLYENYNSYFKADLKAIFERCSKIASKNKVKIYLIGGLVR
                     DLMLGRENYDIDITVEGDAIKFAKILEKEIGAKILSLHKPFGTAKVEINGIKVDFAST
                     RSELYPKKGHLPKVNEIGCSLEKDVLRRDFTVNSLALSLNQQNFAQGLDYVGGFKDLK
                     NKKIRILHDKSFIDDPTRIIRALKYAQRLSFELDKKTFELQKEYLKKINYDMCYKRVK
                     NELKLTFNSKSQNAFETFIEQGIYKLITKKGIELPKINIEKLIAKYRIKQPWIVYVGL
                     IGVFEEDSFSDKLELTKKEKETISASKKLLKLKIKKDDFGIYKAFEGKCIESLIIYYL
                     ISCDEKVLRYLDVLRGIKINIKGQDLIAIGLKPSKEFSQIFDYVLREKLKNSKINKAK
                     EIELVRGFCNY"
     gene            31867..31938
                     /locus_tag="PKI94_03465"
     tRNA            31867..31938
                     /locus_tag="PKI94_03465"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31899..31901,aa:Gln,seq:ttg)
     gene            complement(32218..>33755)
                     /gene="hcp"
                     /locus_tag="PKI94_03470"
                     /gene_synonym="priS"
     CDS             complement(32218..>33755)
                     /gene="hcp"
                     /locus_tag="PKI94_03470"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNW25829.1"
                     /translation="MTSMFCFQCEQTAQGKGCTLQGVCGKKADTANLQDELTSTLIEL
                     ANSNEPSEEVTKTLIEGLFTTITNVNFDDESLKIFTKKVSDLIKRPNDFDIKSVWECN
                     EDIRSLKSLILFGLKGIAAYAEHARVLGYKDETVNNFFYEALRAVSKDLGINDLLPLV
                     LKTGEINLKCMELLDRANTETYGNPEPTKVTTNIKKGPFIIVTGHDLRDLHLLLEQTK
                     DKGINIYTHGEMLPCHAYPKLKKYPHLKGNFGTAWQNQQKEFDGVPAAILFTTNCIMP
                     VKESYKDRVFTTAVVQYPETVHIDDKKDFTPVIEKALELGGYAEDKKMTGINGGDVLT
                     VGFGHHTVMSVADKVIEGVKSGAIKHFFLVGGCDGAKPGRNYYTEFVKQTPKDTIILT
                     LACGKYRFNDLDLGDIGGIPRLLDMGQCNDAYSAIQVAIALSKAFNCSVNELPLSLVL
                     SWYEQKAVCILLTLLYLGIENIRLGPSLPAFVSPTVLNVLVDKFKIKTITTPEEDLKA
                     ILG"
BASE COUNT        10368 a         5872 c         6312 g        11203 t
ORIGIN      
        1 tttgatttct gtgacaagcg aattcagcga tgaattataa ttttgcgcag aggctgcaat
       61 tttttcttcg atttggctga cggtttgttg taccgaggtt ttaaattctt caacattgtg
      121 tgctatttca tctacgattg tattttcaag ttctgcaatc tgggtttttg cctcttcgtc
      181 ttggactttt aaaatttctg ataaatcaag cttatgggaa gcaatttctt cctgtgcttt
      241 tgtaatctcg tcgattaatt cctgcaggtc ggcttttaat tttgtgatgt gaccttcgtt
      301 ttcttctttg agcatgtctt ttaggtcttt tttcatcacg tctatttctt caagaagtcc
      361 gtttttaaga gaaatcatct cgtcaagatt gtcttttgaa acatcaatat tttcgtcaaa
      421 tctttcaaaa aggcttgctt ttatgtcttc ctgattttgg attaaatcgg atttcagttc
      481 ggaaattttt tcgataggat tgaaatcttc aagtttttca agaatttttt ctcttgtgtc
      541 tttttggttt ttgcctaaat ccgcaccgag tgaatcgatt ttatcaagca gtttgtctcc
      601 gaaaatgccc attgccgctt gaactttttg agggctttct tctatttttt cactaaattt
      661 tataaattcc gtgtttatag cttgaatttc tgtttttaaa tcctcaaagg atttatcaaa
      721 atttacttcc gaaatatttg acaccaattg gtttacttcg cttatttttt gggacaaatt
      781 tataaaattt tcttcgcaaa agcttttttg tgaagttttt aattcttcta atttttctgc
      841 catttcggca aattcgccgt caatgatttc caatgttttt tggaagtttt cgtcttcctt
      901 aacggcagaa agttctttca tctgcgttaa gttaagcaaa agtccgtcga tttttcgtcc
      961 caattcggtt gacaaattac tcattttttc ttcggaaatt tttaatccgt cttgaatttg
     1021 tgtgaagttt tgggttaatg tttctaagtt ttgggcattt tcgctgctta aattttcttt
     1081 agccgcctca agctttttga taataccgga aatttcttcc attaattctt cttggaaagg
     1141 cttgtctttt gattgaagcc caccgataag gttttgtatt tttgttgaaa gattttctat
     1201 ttcttttgaa ttttcttcaa ttaaaatctt gctttcttga gtgaaagatt gttgattttc
     1261 tttgattgtc aaaggtaaaa ttgaaacttc gtctttgagc tttgaaatct tttcacctaa
     1321 agcctcttcg cttaagtttc ttgcctgttc gatactgtgg gaaatttctt gaattttttc
     1381 tgataaccct tcaaagttta ggtttgactt ttcttcaaga ttttctttta cgccttgaat
     1441 ttctttagag atttcatcaa agtttttgtt cagattttca aaattcgttg acgactttgt
     1501 ttctaatcct tcttttgaat ctttgatttc ttgtgaaatt ccttgaattt ttgccaataa
     1561 gccttcaaaa ttaagttttg attttgtttc aaggctttct tttgtgtctt caagctctcg
     1621 ggtcaattct gttattgtct gcttaaccca tgcggcagtt gttgtggctc cttcttgaga
     1681 ttctgttttg aaagcgttta ggcctgcaat aagttcttca ttgagcgcag aaattttttc
     1741 ggtgctttct tttgaatttt tatctataaa attttgagcc tcagttaatt tttttgaaag
     1801 ttcttcaagt ttttcttcca caagcttgga attttgcgca acatttcctt ctaatagagt
     1861 tcttgtttcg tttgtaaagt tgattacggc ctgaaggttt gtgtttattt cttcaagatt
     1921 tttcttagaa ttttggatta gagcagattt tgaagcgttt aaatcttttt gaagttcttc
     1981 tatttttatt tccacatttt cggaattttc tatatttgat aaatttattt cattttttgc
     2041 agaaaaaata tttgaaataa cactttggat tccgtctaaa atgtttgtca gattagattt
     2101 tgaattttct gaaactgttt tttcggttgc gtcaattttt tccgtgattt gtcgggtgct
     2161 ttggattatg ttttgttcaa gttcgctttt gttgtttaaa gcagactgta taaagtttga
     2221 cgaagcctct gaaatatttt gagcaagatt gttttcagaa gcctttacgg tatttgcaat
     2281 ttcttcaaaa tcgtttttga ggtttgaaga attgataaat gcggtcaatt tttttaactc
     2341 cgaatttgta tctaaaagat ctgctctcat aagatttgaa ctttgcataa cctctgcagc
     2401 aaagtctgaa agttcttttc tgaacccgat aaattcttcc tcagatgaaa tgtttgcgat
     2461 ttttgattca agattttcaa tttgggcact gagcattttt tggttttggt cggttgcttc
     2521 gctgaggata ttttttagtg cattaattac tccgacagtg gtgtcgattt ttttgttcaa
     2581 atcttcgaca acttcattta cggggagttt gctgatgtaa tcgctaagct ggatgttttg
     2641 ggcgacaagt tttgcgactt gatcctgttc gattttaaaa tcttcttttg taacaacctg
     2701 agttaagaca tcttccagtt ccctgttttt gcggtctatt acctgaaggg tcgataaaac
     2761 agcattagaa gacagaaaaa tattttcaat atctttttta acatcagcta aagcctcgct
     2821 ttgatcaagc ttaagaaggc tttcagacat tgtttggaaa ttgcttccca agttaaggat
     2881 gattgattca aatccgttgt tgaatttttc caattcgacc tttaaaagcg aaatgttttt
     2941 taggatgtct tttttggcag tatcgtcaag cttggattct tccatttttg tatttatttg
     3001 attaagcagt tctttttggg taaaaacttc tttggaaaac acgttaaggt ttgtggcaat
     3061 aatgtcaaaa acttgtttta tgtcttgcgc ttttacactt tcgcctgctt tttcgacaag
     3121 ctgtttataa gcttcttcca tttctacaaa tttggaagaa acaaaattgt gtctttcttc
     3181 aaggcttctt ttgagttctg caagaaaaac ctttatcaaa tcggtatttt cttcgtcgga
     3241 tatagtgtcc agcttaaggt taatatttgc caaaacctta tcaacatccc ctgcgtttgc
     3301 cgcattttga gctctcatcg cttctaaaag gttattgata ctttcaaact tatactcaat
     3361 attttctaaa ttcttgtctg ccattaactt ctcatcccca taaataccac agagtatatt
     3421 ttaccaaagt aatagcccga tgtgaaagag attgtaaata aaatttttcc gtcaaagaag
     3481 gcaaaacccc aaaaccctct ggctacaaag gtttcactat ttattaaata ttacgattgt
     3541 aaagttcttt ttttatatta agcaaatatt tctaatttga tacttattgt gttaaaatat
     3601 attggatatg agtttaattt tggggataag atgagcggaa taaaaattga caaaacaaag
     3661 tgcaaagctt gttatctttg tgtcgaagtt tgcccgaaaa aacttataat gataggctct
     3721 gaagcaaatt ctctgggaca ttttccggtt gaatttgttg acgaaaaaaa tgagtgtatc
     3781 gcatgcgcca tgtgtgcaat gagatgtccc gatatggcta taaccgaggt agtaaatagg
     3841 aaatattgaa tagaagttta aaaaatctat ttatcattta ctaaaacaaa aaggcaaaca
     3901 aatgaaacaa ttaataaaag gtaattacgc aatagctcaa gccgcggtaa atgcaggatg
     3961 tgactgttat ttcggttacc cgataactcc tcagagcgaa attggcgagt atatgagcaa
     4021 aaaaatgccc gagcttaaaa ggggctatgt ttctgcagaa agtgaacttg ctgcaataaa
     4081 catggttttg ggggcttgct caactggggt taaggcgatg acttcatcat cttcgtgtgc
     4141 ggttgcactt atgcaagagg ctttatccta tatagcatcg gatgagcttc ctgctgtttt
     4201 gataagcgtt atgagagcag gaccgggact tggatacatt tacccttcgc aggcagatta
     4261 taatcaggcg acaatcggcg gcggaaacgg cgattacaaa ataattaccc ttgcgccttc
     4321 caccgttcag gaatgtgttg atttgaccta taaagcgttt tttcttgccc ataagtacag
     4381 aaatccggta attcttcttg cggacgggct tttggggcag atgatggctc cggttgaaat
     4441 cggtaaatat ccgtatcctg aaatttcaaa caaagattgg gctcttgaag gagcaaaaaa
     4501 cagacaatcg agagcgattt tttcgcttga gcctctggaa gaaaatttga ttaaaagaat
     4561 tgataaattg tttaaaaaat acaaaaaaat cgaagaaaac gaagtcgaat tcgaagagta
     4621 ccaaacgcaa gatgccgagc ttattgtggt gtgttttgga agcataacaa gaaacgtaaa
     4681 atccgcagtt aaagaatgta gaaaacaggg caaaaaagtg ggaattttaa gaccgataac
     4741 tctttggcct tttccttcaa aaaaaatcga agaacttgca aacaaaacaa aaaaatttct
     4801 tgttgtagaa atgaacatgg ggcagatgct caaagatgta aaacttgccg taaacggtca
     4861 agttcgggtt gaatttcagg gcaaatcggc agggcagtta attggagttg aagaaattgt
     4921 ttcgaaaatc gaaaggctta taaatgattg aagtcaaaga aaataaagta tttgaaatac
     4981 caaaatcaat aaaaaaaggc gcaaaattcc cgttttgtcc cggatgcgac catggggtag
     5041 caataaaact tattgcagaa gtcatggatg aattgggttt aagggaaaaa actattgggg
     5101 tttcttcgat tggctgttct gtaatgcttt atgacttttt ggatatagac tgcgtggaag
     5161 cacctcacgg aagggctttg gcagttgcaa caggtgttaa aagagcaaaa cccgacaatt
     5221 ttgttttgac ttatcagggt gacggcgatt ttgcttcaat agggcttgca gagtccatgc
     5281 acgcagcact tcgaggcgaa aaaataaccg caatttgcat aaataacaca acttacggaa
     5341 tgacaggcgg acagggcgga cccactaccg ttatggggca ggttacaaca acttcgccca
     5401 ccggcagaaa caaagagtat cacgggtatc cgattaaaat cgcagaacaa atcgcacttt
     5461 gcgacggaac ggtttatgcg acaaggtgct ctttagacag catcccaaac ataagaaaaa
     5521 caaagcaggc aatcaaaaag gcttttgaaa ttcagcttga aggcttgggc tttgcatttg
     5581 tggaaattct tgcaacctgt cctacaaact ggcgcatgag ccctcaaaaa gcccaccaaa
     5641 gagtcagaga cgagttattt caaatctatc cgttgggaga gtttaaatta gtaaatagta
     5701 aatagtaaat ggtaaataga agtgaatagg tgaataagta aataagtgaa taggttcaat
     5761 ttgttgcgaa gcaacaaaaa tattaacctt tcagccgttc acccattcac ccgttcagct
     5821 aatataaagg aaaaaacatg aatgaaaaca gtataatatt ggcaggattc ggcggtcagg
     5881 gaattttgct tgcaggcgaa gtgcttgcaa cggcttttat gcttgacggg aaaaacgtca
     5941 catggtttcc ctcctacggg gctgaaatgc gcggcggaac ggttaattgc gaagttgtcg
     6001 tgtcggaaga cgaagtaagc tctgtcagca aaaaagaagc cgattttatt gtggctttaa
     6061 accagccttc ctttgataaa tttttgccca aaattaaaaa aggcggctgg ataatcgcaa
     6121 attcctcgct tataaatgaa gaaaaaccga gaaatgacat aaattacgtt tttgcgccga
     6181 ttacaaatat cgcaaacgat ttgggcgagg ttaaaatttc aaacataatt tctctgggaa
     6241 ttcttgcaaa agtaagtaac ttaacttcaa ttcaaagtct gcatagtgca ctcgacagcg
     6301 ttttgcccga acacagaaaa aatcttttgc cgctgaattt gaaggctctt gaaatgggat
     6361 tcaattttgc ctccgagctt gttaagtgtt gaaaatccac cattggacgg atttcaaaat
     6421 ttttaaaaaa aagtattctg ttattattcc gactgaagca tcaagagggt ttctcagtga
     6481 ataaaaaaga tgttgatgat ttaaaaaaaa ccatacagga aaaagaagtt caactggcaa
     6541 agctgaaagc tcatgttgac aaatcttcta tatgctccga tctttacaat aaggtcgttt
     6601 tggaaaaagc aattttgaaa aaagaacttc aggatgttga aaataacact ttgacttcaa
     6661 aaataaaaaa aataattccg cgcaaaaaaa cgcttatttg cgattatttt aaaaagtgat
     6721 tttcgctgaa tagctgaacg ggtgaaaccc tcagaatgac ttttcacatc gttgcgagtg
     6781 cccgaagggc gcgtggcaat cttttgattt tacaaatggg ttagattgct tcgtcagaaa
     6841 taaatttctt cctcgcaacg acgaaaaggc ggatgagagt aacaataagt caaatcattt
     6901 actcactcgc aatgacacat ttctaatttt cacttcgttc aaaaagaact gcgtcattta
     6961 ttcacttttt tgatataata attccatgga aaaattttac ttaaccaccg caatagatta
     7021 tgtaaacggt gcaccgcata tcggacacgc ttatgaaaag attttaaccg atgtcatcgc
     7081 aagacatttt tcgcagcgtt gcgatgacgt ttactttttg acaggaacag acgaacacgg
     7141 gattaaaatc caaaaaaccg ccgcggcaaa agggattagc ccgcaacaaa tgtgcgatga
     7201 gcattttgca aaatttcaac aagcgtggaa agttttggaa gttaattaca gccaaattat
     7261 cagaacaaca gatgataatc ataaaaaaat cgttcaacag atttttaaaa aattgtatga
     7321 gcagggcgat atttacaaac actcgtatca agggctttat tgcagcggtt gtgagagctt
     7381 tttgaacgaa aaagatttaa tcgggggtaa atgtcccgta cataatgttg agcccgaatt
     7441 ggtaagcgaa gaaaattatt tctttaaact caccaaatac aaagatgcga taattaaaca
     7501 cataaaagaa aaccccgatt ttatagtgcc tgccttcaga gcaaatgagg ttttaaatca
     7561 gcttgaaaat atcgaagaca tttctgtttc gcgctctaaa gaaaacgttt cgtggggaat
     7621 tgatgtactg gatgattcca cccagataat ttatgtttgg attgacgcgc tttctaatta
     7681 tataaccgca cttggttatg atgttgaaaa accttgcgat accttcaaaa aatactggcc
     7741 tgccgatgtt caagttatcg gaaaagacat tctaaaattc catgcgattt actggcctgc
     7801 aattttgctt gcattagact tggaacttcc taaacacctt tttgtgcacg gatttattaa
     7861 tataaatcag gaaaaaatgt caaaatcttt aggcaatgtc atttctccgg ttgaaattat
     7921 gaaaaactgg gaactgcaaa cgcctgatgc cctcaggtat tatcttgcaa cagcggcacc
     7981 gttcggcaaa gacggcaact tttccgacga agattttaaa gaaaaagtca acgccgattt
     8041 ggcaaataat atgggaaatt tattaaaccg cacgctttca atgctggtaa aatactttga
     8101 aggaggggta aatgtacttt ggaattcaga taaatgccct ataaaacctt tagaaaccgt
     8161 tgaaaaagtt aaaaaacttt ttgataattt tgaaatccag gaagccgcaa acgaaataat
     8221 ttcacttgtt gatttaacca acaaatatgt tgccgaaaat gcaccgtgga gtttagcaaa
     8281 ggaagaaaaa tgggacgaat gcggacaggt tttaactaat gttcttgaaa ttatgtgtgt
     8341 tgtttgcgtt ttgatttatc ctttctgtcc taatatcgca caggatatgg caaatcagct
     8401 taaatttgat ttaaaaacaa aacttgacga tttaaatttg aataacataa agcagggcaa
     8461 attaatagaa aaagaagata taaaacctgt tttcctccgc cttgacagcg aatttgcaac
     8521 aaataagggg aaaatgtaaa agtaggttgg gctttccagc ctaacaacaa aaggtaaata
     8581 atggaaacag aattaaaaca aaagtttaat aatctgaagg ctcgtgtgca aaaagcacaa
     8641 gactgtcttt gaccctgaaa aattaaaaaa agaactgttt gaaattgaat ttaaattgca
     8701 attacccgaa atttgggctg atcagaataa ggcttcttct ttaggggctc aggtacgaga
     8761 aattaaagag aatttggaat taacctcacg ctggcagaat attcttgacg atgccgaagt
     8821 ggcaatagaa ttaggcgaag aagacttggt tcaagaaacc tttgaaaacc tttctaatct
     8881 tgaaaaagag cttgacaaat acgatatcag gcaaatgcta tcgggagaat acgatgatgc
     8941 cgatgcgatt ttgtcgataa atgcaggtgc aggcggcaca gacgctcagg attgggcgga
     9001 gatgcttttg agaatgtata gccgttgggc agagggcaaa ggttggaaag tagatttact
     9061 cgacaaatca gatggcgaag aagcagggat taaatcagca acaataaaaa tttcaggcaa
     9121 gtttgcttac ggttacgcaa aggcagaaaa aggggttcac aggctggtga ggatttcgcc
     9181 ttttaacgca aacggaaagc gtcagacaag ttttgcatcc tgtgaggtat cgcctattat
     9241 tgaagatttt gagaagaaaa ttgttattcc gccagatgat attgaaatcg agaccatgcg
     9301 ttcaggcggt gcgggcggtc aaaacgttaa taaggtggaa actgctgtaa ggatttatca
     9361 caaacccaca ggaattgtgg tcaagtgtca gcaggaacgc tctcagcttc aaaacaggga
     9421 aaatgcgatg caggttcttg cctcaaagct tcttgccctg aaacaggcag agcatgaaaa
     9481 gaaacttgca gaattaaaag gtcagaattt tgacataaat ttcggctcac agattcgttc
     9541 ttacgttttt cacccttata aaatggtcaa agaccaccgc acaaactttg aagtcggaaa
     9601 cgttgatgct gtcatggatg gcgacctcga cgggttcatc gagatatttt taaaaagtta
     9661 gggctgtttt tttaatcttg cttcaaatcc ttttttaacc aaaaagttca tccaattttg
     9721 cggcaggtgt gacaagagtt gtgcaaaaaa tgcgtcttta cctattgtat aagaggcttt
     9781 ggggttttta cattcgctta tttttacaat aatttcagct actttttgtg cctctaaacc
     9841 ttttttatta ttcttttggg cattttttgc caaaaattca aattcccttt ggtacttttg
     9901 atttccgccc aaatcctctt ggttattttt aattgattta tcccaaagcg gggtttttat
     9961 aacacccggc tttattgaaa tcacttttat atcgtgtccg cattctatct gcatcgagtt
    10021 aaacaaaata tccaaagctc ttttggatgc acaatatggg gcaatgaaag gaaatatccc
    10081 gaaacttgcc attgagctta tattaataac tcttgcgccc tgcaattttg aaaaaagccc
    10141 ctgcgtgaat tcaagatgag aaaaagtgtt tacttcaaac tgttctttta ttttatcagt
    10201 atttaaacac tccatagcac ctgcaaccac aaagcctgct gcgtttatta aaacatcgat
    10261 tttatcagtt ttttcacaga taaattttgc cgcattaaca acagtttctt tttttgacat
    10321 gtcaatataa aaaggaatga cattttgcga aatttgctcc aactctttta taaatttctc
    10381 attcctatat cctgcaaaaa taagacagcc catatttgca tactctttta cgagtaaatt
    10441 tcctaatcct gatgtcgccc ctgtaattac tatcgttttc aattctattt accgtttatt
    10501 cgtcttctgt tgtcggtctt aaaagcacaa tcgaattcag gttaagctga agaaagttat
    10561 gatattctgt tcttctgttt gtaagaaagc gatatgaaat ttcacaatct tttatcgcaa
    10621 caaaacgttt cccgccgttg aggatgtctg aaagaatacg atttttcttt cctgttttag
    10681 gcataaaaac gtagcccttg atttcgtagt cttgagttac caccaaaacc ttttccgacc
    10741 aaactcctga aataaaatca ttttcttctg tcataatgcc tccaatattt aatccgtcat
    10801 gtacgagctg aatagcggta aataaagcgc aagtgccaac acaagtacaa tacttcctat
    10861 aacgataagc attataggct caaccatttt tgtcattaaa tcaattattg catcaagttt
    10921 tttatcgatg aaagctgttg cctgcaaaag catttcaccc agcctacctg actgctcacc
    10981 tgttgcaatc ataaacaaaa tcatcttggg cattacttga gtggagcgca aggcaagcga
    11041 taagtgttgc ccctgctgaa cctttgttgt tgcctgtgat actttatctt gcagtgtata
    11101 attacttatg gttaaatttg acaggtaaag acaatccaca atcggaacac ccgcatcgta
    11161 tgccacctgc attaccgcaa taaagtttga aaaattggaa aattgtatta aatccgataa
    11221 tatagggact ttaagaacaa attcgtctat ttttcttttg cttggctccc aattaagcaa
    11281 gaaagtaaca ccaaagatta ccgagccgaa aattatcggg attaaaaccc agtaagtttt
    11341 taaaaattct ccgatagaca ttaatgcagc agttatccat ggcagaggtt tgccttggtt
    11401 gtcaaacatg tctttaaacg caggaaatac aaatacaagc atgaccgtta ctactaccat
    11461 tgccaacaaa attacaaaag ccggatacat aagggttccc acaacttttg atttgattgc
    11521 agcttctttt ttcaataatt ccaaaagcct gtcaagcgtt ttttccaatt caccggaatc
    11581 ttcccccgct cttacaagcc ctatgtaaat ttgtccgaag attttgggat attttgcaat
    11641 ggtgtcggca aaggttgaac ctgccataat ctgtcttctt aattcttttg ccacaagtct
    11701 tatttttaat tttgcagcat cattttccat aaaaacgagg gcttcaatga ccggaattcc
    11761 tgattgggta agtatttgaa aggtagaagt aaaatctatt ttgtcctgta agcctaaagt
    11821 ctgaattttt tttgcatcaa cttttgccac ggtttttgct tgagattctt cgtaaacttt
    11881 tgtcggaatt aagttcagct ttcttatgtg gtcgcgcgct tctttaatgt tagaagcttc
    11941 aattttgcct ttaaccacat cattattatt ttttagtgct acgtagctaa ataccgtcat
    12001 ttaattcctc gttattcatt tgccaagcct aataccctga caaattcgtc aattgttgta
    12061 aacccgttta ttatatgact taaacaggct tgattaagag ttttcatgcc acaggcgatt
    12121 gcggcttctt ctatgtcgat atcatgtgcg cctgttgcta ttaatttttt tatttctttt
    12181 gttatgggca taatttcata tacgcctaaa cgccctttaa accctgtata atcgcaatta
    12241 ctgcaaccta taggtctgta aactttggtt tttgtgaatt tttcaatttc gccttcgtca
    12301 atcaaaattt ttgaagcttc ttctttactt ggaaaatagc cgtctttgca gtcgtcacaa
    12361 agttttctta caagcctttg tgctattacg cccgataaag tagaagatac aagataatct
    12421 tttgccccca tttcaaccag tcgagtgact gtggcggcag ccgaatttgt gtgaaccgta
    12481 cttaaaacaa ggtgaccggt aagtgctgca gaaattgcaa cttcaagtgt ttcgtaatcc
    12541 cttatctcac cgaccagaat aatatcaggg tcttgtctta gtatggctct catgcaagac
    12601 gcaaatgtaa tccctgcttt tgcatttacc tgagactggt ttagtccttc cagctttatt
    12661 tcaacagggt cttcaattgt tgtgatgttt acttcttcat cgtttaaact tttaagcacc
    12721 gaataaaggg tcgttgtttt acctgatccg gtcggtcctg aagttaagat tataccgtta
    12781 gggcagcttg tcattttttt aattttttca atatcctcag gcatcccgcc cataagatga
    12841 atttctttgt cggaagaatt taagcttact gcaggtgcaa gaacccttat acaaactttt
    12901 tctttgccgc ccacaggcag ggtgtttatc ctgaagtcat aagagcttcc tttatatttt
    12961 aatgaaaaag ttccgtcctg aggtcttctg cgctcggcaa tattcatcct tgaaataact
    13021 ttaaaacgtg ttataaccga catctcaact cttggaggga cttctataat ctgtcttaaa
    13081 ataccgtctt ttctgtatct tacaacatag ttttcaagtc ggggctcaat gtgtatgtca
    13141 ctgactttat tgtcaatggc atctgttatt attttattta caaattttac aacagttcct
    13201 gaagtatctt gaagttctct ttcaacctga tctgcaagac tttcttcttc ggtaaactcc
    13261 agattttctt cttcaatttc ttttataatt ttatttgttt ctttttttgt tgcgctgtat
    13321 tctgtttcga caaaactttg gaattcaaag tgtgtgatta acatggcacg gggtttttga
    13381 cctgtaagaa aaactatatc tttgagaact tttttattat caggttttac catccctaca
    13441 acaagagtgc ttccgtcaga agatatcggt attactttgt ttgcccttac aaaatcttca
    13501 ggcagcatgt ttattacttt ttcctgaact tttagttggt ctgaatcgac catatcataa
    13561 ccttgttgat ccttaagagt ttcttttaat tgcgaaagag ttataaaacc taacttaaac
    13621 aaagctgagc ccaaaggcat ttttaatctt ttaccttctt caagtgcttt ttgaagctgt
    13681 tcttcattta tatagccttt ttctataaga atttcaccta tttttttctt aggtgcctct
    13741 tctccgtttg cagggttttg ctcttgagtt tgtccttgag ttttattttt tgcttcttca
    13801 agaagttttt ttattccgct gtcgtctccg taaacgtatt ttaaaagagc atcaacttcg
    13861 acttctgcaa tttgtataaa tttaacatca acaatcccca tgcttttgac gacttttgca
    13921 attgtttctt tttctgcaga aggatttgtt gcaacataaa ggcactctcc tttcatgttt
    13981 ataggtaaaa attggtaatt ttcaaggata gttttattaa actttgcaac aatatcttta
    14041 tttatgctgt ctttgagttt ttctaatacg ttttccattt atagtgttgc accttcattt
    14101 tggaccaatt catcattatc ttttattatt ttcggtgtaa tcattattac aagctcttgt
    14161 tttgattttg tagtgttggt gttgcggaag aaaaatccga cacctggcag gtcgcctaaa
    14221 acaggaattt ttgaaacact tttttgttca tcttctctta tcatccctgc aagaacaagg
    14281 gtctcgccgt cttttatcct tatattttta agatcaagat ttcttctttg aaggagcgta
    14341 acagccaaat ctttaacggt cggatcatcg gcatttgggg tgtatatttt ttcttttacg
    14401 gtagaatact ccggcttgat atttaacgtt acatagccgt cagggcttat aaatggaatc
    14461 atttctactt ttatgccttc atcggagcct atttcgtaag ttttttgaac ggcacctgcc
    14521 atcccgccgc tgtttgcaag aacttcagat ttaactgttt tcacataatc tgcagtcaaa
    14581 tcaacggttg aggtttgtcc gtttgttatc atgattctgg ggtttgcaag cactcttcct
    14641 ttttgatttt gaattaaata gttaatagaa taaaccaaag aaggtgctcc cgcatattta
    14701 agaatttttt gtactgtagc atcatcacct gttttcatag gatctgtaac tatgtactca
    14761 tcatttctga aaaacatagg ataataagga tttgattttg tcgttccgtc aaaatttgcc
    14821 gagaaatttt tactcaaata ctgccatgta ttactgaaat ctctcattcc tgattcgttt
    14881 aattcaataa tggaaagttc cagatatgcc tgaggttgtc taacatcgtt ttgtgcaata
    14941 aattctttta tcatttcgat ttggcgttgt gagccgcctt taattgaaat agttcctctt
    15001 tggggaaaat aagaaataat catgcttttt gaattaaaag atgagatatt tcctgcagat
    15061 attttattat catattcgca ggcaataaca ttttcgccca aagcaagttc tccagaagct
    15121 gcatctgcgg ctccgccggt tgccttgccg cctgcgctgc cgccggagga cgcccctgaa
    15181 gggggaaaca acacggtaca aagaagcgtt gccatttctt taggtgttgt atggtttaca
    15241 gcaaaggttt ctgaagaagg tttgacatca aatttttcaa ttattttttg tgccatttta
    15301 acgtcattat ctgttccgaa gattaaaatt tcgttagccg cagggtttgt tattgcgatt
    15361 tgtgaatttg aaagcccggg cttgtttatt gagaaaatgt ttttgttcat aaattctgcc
    15421 aaaagagaag cttctacgta cttaacagga attgccacca tttcttgttt ggaaaggttt
    15481 agtgattgtg ccgcacttgc tgacgctact accatagtgt ttttgtcgat gtagtaggtt
    15541 aagtcgctta tttgcataac aagcttaaaa gcatcgttta agggcatatt aaccaaatct
    15601 aaagttactt tcccgtcaac tgaattgtgg aaaacaatgt ttaagccggc tttatccgca
    15661 aacatcctta atacttgttt tacatcagaa tccctgaggc ttaaacttat tttaggatta
    15721 ctttttgtta ttgaaatttc gccttctagc ttaagcactg tgggcaaagg ttctttttcg
    15781 gcaattttat ttgctcttag ttgaatttct tgtgcacaaa agccaatggg tgctatatat
    15841 gtcattaaaa aagcactgac cataaaacat gataaaattt ttgtaatttt catttattgc
    15901 tccctgttaa ttattttttc ttcttcttaa cttttattga aacatcattt ccggcaaatt
    15961 tcttttccag atttgcaatg gtgttgtaat taatttcact ttgcacaaga agttgtccta
    16021 cacctgcttt ataaacattt tgtcccagtt gtacaacgac ctgatctttt gaaatgccca
    16081 aaactctgta gttgtttatt acatcaccct ttttgaccaa ataatcgttt ccgcctatgt
    16141 ttattatagc cgaagggctg tatctgtcgt ataaaatacc cgagattgtt gtgcccataa
    16201 ctttatctgc atcagagccc gttcctaatt ggtcaggagg atttataagt gtgaatttcg
    16261 gtgccgacaa cattgaaagc tctcctgtag ggacaaaggg attttgtctg cctgattctg
    16321 aaacctgtat taagactctt ttgtcaacgt taagcaactt tcctgcattt tttttagata
    16381 caatttcaac atcgtcattg ttgcttgcgg tttgaggtga gcttgtttgt gataccggta
    16441 tctgaactct tttttgagac tgtgaaggca tattatcagg ggctttttct cctttaagtg
    16501 ccagggtttt agaataaatg tcttgagcat cttgctgtat ttgttctgct gagtctgttt
    16561 gttcaaggct ttgaagtcct ttttgctgag cttctgccaa aagggcttct gaggctgatg
    16621 tgtcaacatt ttgggagtta tttgaatatt gtgtgaaact tgcatatcca agagcacaaa
    16681 caagtaaaat tcccacaaca acaagtgcta tgccctcatt acggtttttt acaaggataa
    16741 aacccttttt ttcttcggca gaattatcaa cgctttcgac gtttttgatt ccgacttcgg
    16801 aatatcttat attctcttct tcattatcca atccgctaac catttcccac cctgataaaa
    16861 taaatacagc tttaattaat tctataattg acttaggtat aaagtatccc ttattcgatg
    16921 tattttattc aaattatacc actaaaataa agcacttttc cccattcaat attttagcat
    16981 tattattatt ttctgtaaaa gatattaact ttgttaacaa agcatttatt ttcgtaaatt
    17041 aaaattgtac aatataatct atgaaagata tttctgtaat cgcacctgtt aaagagccta
    17101 aagacattga ggaatttatc cctcacacta aatgtcgcga tttttatgtt tattacgaca
    17161 ggtttttgaa taacaacttt gagtatataa atgaatttat tgaggctgct cacaaaaatt
    17221 cctgcaaaat ttatataaat ttcaaacatg atatctcaga agaagatttg gttgaaataa
    17281 aaaaaatgat aaggttttta aaaactacaa aaattgacgg aatttttatc aacagttttg
    17341 cggttttaga ggcgataaaa gttttttccc tgccctttaa gataattata gattcttatt
    17401 ttgacattca taatctttcg gggattgatt ttgtaaatac ttttcataaa gttgatcaga
    17461 taattatcac cgaagaaatt tatatgaaaa atatcgcaaa aataaaaaaa tacactaatc
    17521 ttccgcttgc aatagattcg gataatctac cgtggtgcgc tgaagatatt aaaaaattaa
    17581 aagctataga cagtgttgtt attaaaggaa aatttggctc cccggaagaa attttagaag
    17641 gaattgaact ggtagaaaag attttggata agccaaaaat gttcaaaaac caaaaactgc
    17701 cctttaaaca tgtcagaaac agtttttatc ggacagatca tttttcaggt gaaatggtca
    17761 gtgccgaagg tagagatttc aaattcagca gaaatatcca gaaatttgac tgggaaatta
    17821 agcgcacaaa actcaagaaa gaagcaccat ataaaaaaga cccggatttt aagataaatc
    17881 taagactctc aggacttgag cagcttaatc agaccaaaaa atttattcaa aaattaggct
    17941 ataatcctgt tgattcaatc gaatacggtg aaattctttc tacatctgac ctttcaaaaa
    18001 gcagttttaa cgaaattatt gttaaagtta agaagttttg tgaggcgcaa ggaataaaac
    18061 ttcagctttc aacccccaga attttgattg aaagagattt tgacagggtt tttgagtatg
    18121 taaaaaatct ttgcctgact ccgcccatac ccagttcttt gataattaat aatatcggct
    18181 atttttgggc ggttattaac gataatgatt tgaacagaat ccccatagaa atagggcagg
    18241 ggataaatct tttaaacagt atgtctatta cttgtcttaa taatcttcac ccgattgata
    18301 cagttgattt tacgggcttc agtgaggggg aaagtacaat ccacacgatt aaaaaaatca
    18361 aaaatttaat caacaacaga aaatatacgg ttgcagggaa tataagggtt cccagtttag
    18421 ggctttgtcc tttaaacaat gattctgcga taatatcgag actttcgtgc aaagcccctt
    18481 gtcataaagg cgaatatgca cttaaagacc cttctttaaa aaagatttat ccttttacgg
    18541 ttgacggttt ttgcaggatg cacatgtttg aagataaaat tatgcaaaga tttgaccaga
    18601 ttgaagagct taaagatgcc gggattaatg aatttgtctt tgatttttcg gcactgaatg
    18661 caaaatttgt tccgattttg cttacaaatt ttttgaattc tcaaaaataa gtaactgcga
    18721 caattaaaaa aaaattgatt gtgctaaaat gattttatga attttaaaga gagggtaaaa
    18781 aataatgaaa aataaaataa gtttattttt agctttaaca gttgcgattt taggctttgg
    18841 gcttgcactt gatgttcagg cacagcctca aaagaaaaaa tctgttgttg ttcaaaaaac
    18901 agttttacag ccttcggcac ctgtttatgt tgatgcaaag tcgcttgata tagtggcaaa
    18961 tccaacaaaa tatttgaata aaagggttaa aattaatgct aaattcgata aattttcaac
    19021 tttggggctt gattataaac ctgcatttag aagttcggaa aaatacataa gttttttgat
    19081 taaaagagat gacgtaataa atcatgtagt tccgctttca gagatgaaaa tatttatgaa
    19141 gcgcgaggtt gcagaaaaac atatagacct tgaaccgggt gatatagttg aatttgacgc
    19201 atatgttttt tcaaacgcac tcggggatcc ttggcttgaa gtcgagaatt tcagggtttt
    19261 aagttcaaaa gcaaaggata agaaggtaaa tagtaaatag taaatagtaa atggtaaatg
    19321 gtgagaggtg agaagaattt acaagcccat gaacttttca actcttcgac tgttcgactt
    19381 ttcaaccaaa acaccttttt cttttaatag acaaaacata aaatttgtgc tataaaagtg
    19441 ttaccgttta gttattcgga gtttaggaat gagtcataaa gaagtttttc ttgcaattca
    19501 cggacatttt tatcaacccc ccagagaaaa cccctggctt gaagccattg aattgcaaga
    19561 tacggcagca ccttttcacg attggaatga gagaataaac aacgaatgct ataaccccaa
    19621 ttcggtttca aaaatagttg acagcagaaa cagaattctt gatgttgtaa acaactattc
    19681 tatgataagt tttaacttcg gaccgactct tctttcgtgg atggaaaatt ttgctccgct
    19741 tgcttatgag cgtgtaataa aagccgatat cgaaagtgtt caccaaagag acgggcatgg
    19801 aaatgcgata gcgcaggttt ataatcacat gataatgccg cttgcaagcg agcaggataa
    19861 acaaacacag gtgatttggg gcataaaaga ttttgaggcg cgttttggca gaaaacccga
    19921 aggaatgtgg cttgcagaaa ccgctgttga tgatgaaact ttgagagttt tggcagaaaa
    19981 tgatataaaa tttaccatac tttcgcctta tcaggctttg aaagtcagaa ggctcggtga
    20041 aaaagactgg accgatgtaa gctggggaaa catagatcct gcaatggcat acaggtatta
    20101 cattaaatct tctccaaaaa aatatataga tttatttttc tatgacggtg cgatttcaaa
    20161 atcggtggcg tttgacgatg ttctaaaaga cgggaacaaa tttataagaa ggcttaaaga
    20221 aggggtttcc gaccacaggg aatatcctca gcttataaac atagcaacag acggcgaaag
    20281 ctacggacat cacactaaat tcggcgacat ggcactttct tatgtccttc gggtaaaggc
    20341 aaaggaagaa ggatttaccc ttgtaaatta ctcacagtat cttgccaaat accccagtct
    20401 atatgaagtt gacataaaac agtcttcttc atggagttgt tttcatggaa taggccgctg
    20461 gaaagaagat tgcggctgtt cgaccggcgg tcatccgggt tggaatcaaa aatggaggaa
    20521 gccgctcaga gatgcgcttg attatctcag ggacgagctt gctttgattt ttgaaaaaga
    20581 aggcaaaaat tattttaaag actcatggga tgcaagaaac aattatattg atgtcatctt
    20641 agacagaagc gaaatgacag ttaaaaaatt ccagaaagaa aattttaaat ccgaactttc
    20701 agaagaacaa aaagtaaaag caatggagct tcttgaaatg cagcgtcagg caatgcttat
    20761 gtacacaagc tgcggctggt ttttctcgga aatttccggt attgaaacca cccaaatcat
    20821 gaaatatgcc gcacgcgcca tgcaacttgc gcagaatttt tctaaaaaag atttggaaaa
    20881 acattttctt gaacttcttg atgaggcaaa aagcaatttt gccgaattcg gaacaggaaa
    20941 agatgttttt gaacgcttcg taaaaccttc tattgtaact acaaaacaga ttgcaagcct
    21001 gtgggcggtt tcttctttgt atcaggaatt tgatgacgaa gaagatgtat attgctataa
    21061 aataaagaaa aattcttata aaaaagttca aaagggtact tcgcagcttg ttatggggca
    21121 tatcgaaatc cagtcaaaaa ttactcttca aaaatcaaat gtcatgtttg cgcttgttca
    21181 gtattcgggc ggcgattttc actgcgcgat aaaagaattc agcgcagaga gcgattatat
    21241 taacactcaa aaagaacttt tcagaatctt tatgctcaat cctctgacag agattataag
    21301 agccatggat gaatatttcg gcaaggaata ttttaccctc aaagacatat tcattgaaga
    21361 aagaagaaaa atccttgaaa ttatgcttaa aggcaaaatg aataaatttg cccaaactta
    21421 tcaggaaatg tataacgaag gcaaaagttc catttatcac atgcaaagtt tggggcttag
    21481 tatcccggat gaatttaaaa tttctgccgg ctacacgttg agccgacagt ttaacgattt
    21541 gatagttcat tcgagcggat ttctcgacag caatataatt cagcaggcta tagatattaa
    21601 ttacgaaaca aaaagaatcg gcattaacat cgacaaaaaa cctacgaacc aaatttttag
    21661 ccaaaaaatt acccaaaata tttctcggct tgcacaaagt cttgaaattc agcaggcaga
    21721 agccattttg gaaatttttg acaatgttga aaaacttgaa ttggaagttg acatagctga
    21781 ggctcaaaat atttatttca ccaaaattta tcatgagata ggcgatatta tagaaaatat
    21841 gacaaagaca agcaaagccg aagacagaaa tttcatcctg attctgcttg atataggaca
    21901 aaagcttaat attaatactg aattttaccg ctcaatgctg gataaagtgt tgataaaaaa
    21961 ataaaatgta taaaaattta gaagaattaa ttgaaatagt aagaattttg cgctcgccaa
    22021 atggttgtca atgggacaga gagcaaactc atgcttcact caagcccaat atgcttgaag
    22081 aggcgcatga agccattgat gcaatcaatg aaaatgattt aaagcattta aaagaagaat
    22141 tgggcgatgt tttgttgcag atagttttgc actctcaaat agctgatgag caaaaatctt
    22201 ttaatattga tgatgttgcc aagggaatta aagataaatt aattcgcaga catccgcatg
    22261 ttttcggcga tgtaaaagtt agttcaacaa aagaaatttt ggataattgg gaagaaataa
    22321 aaaaagaaga aaaaccgcac agaaattctg ttatggatgg ggtttcaaaa agccagccgg
    22381 ctttgatgtc ggcgcagaaa ataagcaaaa aagcagttaa ggtaggattt gaatgggatt
    22441 gcattgaaac actttatgag tgtgtttttt ctgagtttga agaatttaag caggcatgtg
    22501 cggttaaaaa ctctgaagaa atggaagaag aaatgggaga tattttgttt gcggtggtaa
    22561 atcttgcgcg ctggaataaa atagatgccg agcaggcact tttaaaagcc aacaaaaaat
    22621 ttataaaaag gtttaagcaa atggaaaaac ttgcgacaaa acctcttgaa gaatattcct
    22681 ttgaggaatt cgacgcactt tggaaatgcg caaaaaaaac aaataattat aaataatttt
    22741 tttagtttgt tatgttaaca ggcttaaata ttgattttat tttttgctta aaatataatt
    22801 aaaacagcca aattttaaaa atgaaaatat gacaaacaac aaaaatccaa aagaaaaata
    22861 cagtgttttt ttaaaacacg ttgaagaaga aaaagaggaa acatcaaaat ttgcaaaaac
    22921 tttgctggtg cttgtttttc ttgtttgctc tcttgttgtg gtttttttgt ttttgcttga
    22981 tgatcaagaa acagttgagc aattgttcgg acgtgataat attgtttcgc aaaaacttgt
    23041 cgaatttgtc caaagccgtt ctcacaaaca tcgtgccgaa ttttctattc cgtttttacc
    23101 ccataaacaa aatattttac ttttgggcgt cgattcaaac ggttcagata ccgatccttg
    23161 ggagggcaca cgttcagata ccatagtttt attgaatgtt gatccaaaaa cacgctctgt
    23221 taacgcaatt tctatcccca gagattcaaa agtttatctt gcaggaaatt ttggggttca
    23281 aaaaataaac gccgcacacg cacttggcgg aatcaagctt gccaaaagaa ccgttgaaga
    23341 ggcactcggg gtaaaggttg acagatatat tgttgtaagc gacgaggctg tcagaaaaat
    23401 cgtagatgca atcggcggtg ttccgattta tgtcgaaaaa aatatgtttt ataacgatta
    23461 ttcgggcgac ttgcatgttc atcttaacaa gggattgaat attcttgacg gcagaaacgc
    23521 agtaggatac ttaaggttca ggcatgacgg actcggtgat ataggaagaa ctcagcgtca
    23581 gcaatggttt ttgagaggtt tgttggaaaa aatccaaact ccgcaggcaa taatgaaact
    23641 tccagagatg ataaatatcg cttcaactta tgtgaaaacg gatttgtctt tgtatgaaat
    23701 gtcgcagtat gccgctatgg caaaaagctt tgatgtgagt aaaattgaag ttgcaacgct
    23761 tccgggtgca ccgaataaaa aaggttatac aagttattgg attttagacc ccgataaaac
    23821 tcaagaagtt gtaaacaggc ttatatatag agacagaccc gatatttcgg ataaaaaatt
    23881 tgttgcggga attatgtatt ctcccgaaaa agagcaggaa gcaatggaag tcaaggcaaa
    23941 acttgaagaa atgaattttg aagttaactg tgtcggcaga gggcaccttc ctcattccca
    24001 atttgttgca aattcaagca gtgttacaag tgaatttttt aactgggtta aaagaaaaat
    24061 tcctgcaata agaagaagcc aatttgttta tgatcccaac aaattttact gtgtaaacag
    24121 cgattttgta attattttat ccggtcaata aattttaatt atttagtgtc ctctattgaa
    24181 acaaattttt atgttttgcg tctgattaaa agaggcagtt tttgccttta taatcagagt
    24241 gttagttgtt gaggtatttt ggaggattta agatggccag acagtgtgcg ctaaaggctg
    24301 aaaagcctaa taatttattt aaaagtttac agcaaaaata tattgatgaa acagattggg
    24361 aacaaataga aggagaagat ttaattcagg agtgtaaaac atttaattct atttcaaatg
    24421 aagatgatat tctgcagatg tatctaaaag atgtcggaag ggtgaagctt ctatcgggca
    24481 atgaggaaaa aagtctggga aaaataatta aagaaaaaag ggacattcaa gcagatatcg
    24541 ccaaaagaaa acttattcag gcaaatttgc ggctggtggt gagcatagca aaaaaataca
    24601 tcggacaggg cgttcttttt atggatttgg tgcaggaagg ctctttgggg cttatccgcg
    24661 cggcagaaaa gtacgattat tcaaaagggt ttaagttttc gacttatgca acctggtgga
    24721 taaaacagac tattataagg gctatttcaa attattctcg ttcgattaga attcccgtgc
    24781 atatgacaga taaaataaga aggtacaaaa agaccgtgtc agagcttaat ttcgtacttg
    24841 gcagagaagc tacagaaaaa gaaatagcag aaaaaatggg tttgaacgta aaaaaaattc
    24901 tttcgattaa gcgttctata attaaagaac ctgtcagcct tgatagtccc gtgactgatg
    24961 atttgaatgt agaagactat gttcaagacg gaaaaagcag ctcccctgag gaaaaaacaa
    25021 gtataaacgc tctttgtgat ggaatggagt gcattttaga cgaacttgac gaaagggaga
    25081 aaaaaattct cacctacagg tttggaataa acggagtcca gacaaaaacg cttgagcagt
    25141 tgggaaattt aatggggttt tctaaagaaa gaatacgaca gttagaagac ggggcgatta
    25201 aaaaactcag gcaacaggaa aatattagac atttcaagga ttttataaca agcgaagaat
    25261 agattaaaaa taaggggcgg gttcaaaaat ttttgaaccc gccccttatt gtacacttgt
    25321 ttatatcata ttttttctat atatctttta acatcaaact tatttttgca gccttgcgca
    25381 ctcatgtagc ggattttacc cataacagga cgtgaagaaa aagccctgtc atgaactcca
    25441 acgattgacc ataaaatccc tacgtatccg tttgcatcgt ttccgtcgag tgcgtatttg
    25501 tcgttgagat aaattgcagt tttaagtgcc tcttcaggac tctgcgacca ttcaaggatt
    25561 ttttttgccc agtacattct catatatccg tggatttttc cgttttgaac taaatctttt
    25621 tgtgctgcgt tccaaagttc gtcgtgagtt tttgcatctt caaaaatttt taggtcataa
    25681 acataaggtc ttatatcaaa tttgtgctct tccaatgtct ttttagccca agcaggagtg
    25741 cttttgagcg atttgaaatc ttttgaatac aggcaaaaat tatcagaaag ttcctttctt
    25801 acgattaatt cttctaaaaa agcctcttta ttttctcgtg aggcgtttga ttttaaaact
    25861 tcaagggcaa ccctttgggc ggaaatctga ccgctgtgaa gatagggcga aagccctgag
    25921 acaaaatttt gtgtcgggtc attttttaat tcagtgtaat aattaagctt gttttcaata
    25981 aaatcaaaca ttttttcttg tgcttcggat ttttcgacag gaaaagggtt tttaaattct
    26041 gtcaaaaatc gtccgatatt tacataaact tttcttctta aagttgctgc tgaaaattct
    26101 tgtttgtcag agataaacct tgcaggaatt atgttgtggg aatctatttc aaaaatggct
    26161 tgggcggcaa aatcttttgt aacaaaagat tttgccgcct tgtcaaattt taaaacagga
    26221 ttaaaatcaa taactaaagc cccaaaaatt tttaaatatt tttgcaaatc atcttttata
    26281 aattcaaaat cgatatcgtt tttctttgca attttactta aatttttgac gtgaggcagg
    26341 ttaaattcaa tttgcctttt tgaataaatt ttttcatcca aattgcataa aactttcaaa
    26401 tttttgcctg ttttttgcgc aaggttatgt gcaaaaataa agccccagtt gtcttgaaag
    26461 cgaagttccc tgctcataag atagacaaca gcccccgagc cgattttgtc gtttaaggta
    26521 aaaattcttg catcatcgat attttcagga caagaaaatt catcagaaac aaaatctaac
    26581 atcaaatttc ttttaacaga cggttttagc atttagagat tatatcgcta aagaaattaa
    26641 ccttcaatat ctaaattggc ggtatctaaa tcattttcgc cttcagcttc aggcattata
    26701 ttttcgtccc tttctcttct atatacttgc atggattgtt ttgtttcttg gtagttatct
    26761 tcaacaggct gttgaggaag ctcgggctgg gggatatatt ggcttaattc tgtaactgac
    26821 tttttttgag gttgtaagtt attatttttg ggtgcaaggg acaatagact tattaataag
    26881 gtcaatgccg ctattcctgc agcttcttgc attaaaactt tattatattc aggacttaca
    26941 tggttttttt gttcggtttt tttgccaaag ctcatgttgc ttttaacagt ttggttgtag
    27001 taattttgca cagaattaat tctcataggt ttcctctttc atttttatct tctcggttgc
    27061 tttaaacctt ctaaaaaatt tttttgctag ttttgagaaa aatattttta ctttcaaagg
    27121 caaaaattca gttataactg aatttttgcc tttgaaaaac aaattaatat ttcttaagat
    27181 ttattcaatc aaaactctgt ccaggcatgc ttttggcgag tactgccatt ctcggaaggt
    27241 tatgcggtta attttatagc cggaacgctc aagaatcgat tgttttttaa gattacttcc
    27301 tgaaagtttt ttggaatctt ccactccgtc acattcgatt gcaattttgt cgttgactaa
    27361 caaatcaaga ctgaagcctg caatttcaac gcctgctttg acttcgtgtc ctaaatcacg
    27421 gattgcctga gcgatttcac gctcaaaatt atttttataa aagttttcgt caatttcttt
    27481 attttcaata gcctgttgtt tgttttgata ttcctgaata tagctcatat agcttctcaa
    27541 aagtccgtcg ggaagctctt tgggatctct tgaaataaag ttaatcattt tgtgccgtgc
    27601 ccttgtaatt gcaacgttaa acaaatttgg tttttgcagg aaagttaagc tttggacaaa
    27661 actgttgctt gcaaaagccc aggagataag cattatatct ctttcatcac cttggaacgt
    27721 gtgcgcagta cctatttcaa tactgtgttt tcgtatcatg tgctcggaca gaattcttgc
    27781 aacggaggtt ttgagctgtt cgacttgggc cctgaatggt gaaattatac cgatagaagt
    27841 cggattatct ggattcttgc gttcatcttc tataatgatt tcctgaagtt ttttcacaac
    27901 cgcttcgact tcgggaaggt ttcttgttgc gtcaaaatca actttgccgt ccggaacata
    27961 cacaagctca agaactttct cgtttttatt tcttttcatt attcttattc tgctgccgta
    28021 gaattcatca tttgaaaaat taattatagg cggcagactt ctgaaatgtt cgtccaataa
    28081 aacagggttc attgaatagt aatttgccaa atcaaacatt gaatttgtcc tgaatctcca
    28141 cattaactga tatttatcgg gaattgaata ttggctcaaa aacgactgtt cttttgcttt
    28201 ttccaaaaag gaaaggtgcg gaagctgctt gtcatcgccc actacaaccg ccctttttgc
    28261 cctgtaaagg ataggaaaac agcttgcaat atcacactgg gaagcctcat cgataatcgc
    28321 aacatcaaaa agtccgggct tcatcggaag cgagcccgaa atggcataag tcgtcacaca
    28381 ccagcatgga aaagcttcca gcaagggctt aaaatcttcg ccttcaagaa gcctgttttg
    28441 aagatttttc tttctttcta caagcgattt tgtgtgtacg ataagtcttt gacgctttat
    28501 ttggtcgcgc aaaagtcctt taagtgattc tctgcgcttg ctttttaaaa tatcaatggc
    28561 taaatttctt tgtttgcgct taagcatacg gatttgctct gccataacgt gcagattgcc
    28621 cagtttttga atatttgctt caatcaatcg catttcgcat gcggtttttg aaaattcaag
    28681 ttcctgagcc agtctgctta aattttcgta attttcattg aaatcattca attttaataa
    28741 ttttttaagt ttatttttac tcgaatagtt tgaaagtgac acaaaaaacc ctgttttttc
    28801 aagatttttt tcgattgtgg agataagatt ttccacttgt tctatttcgg cttttttaag
    28861 caattctttt ataaattgag tctgccccaa agctttttcc tgctcgtttt tttggacaaa
    28921 aagttcttgc cactcatgtt cgagcttgat aacctgctca cagcggtttt ccaagtcacg
    28981 tagagtgttt acaagatctt tcatatcttt tacatctgca aaaatggcgt tttcgtattc
    29041 gctgtccaaa tcaactttat tagaaagcaa atcctgaagt tgaaaactta actgtttttg
    29101 gtaattgagc cttccggcac gaagcgcaag aaaaggagca ccgagttcgt taagcctttc
    29161 tgatacaaca tcaacggctt tgtccatgcg cgatgctacc agaatggttt ttccgtttgc
    29221 gattaaatgc gcaaccaaat ttactatcgt ttgagatttt cctgtcccgg gaggtccgta
    29281 aacggcaaca aaatggttat tttcaagact ttttataacg ttttcctgtg aatcgcttag
    29341 ggataaaggc gtaacagcaa caaaatcctt caaatctttt aattgcttaa ttggattgtc
    29401 aacaagcttt ccttttgtct gcaaatattc ttcgttaata atattcaaag cagtttctcg
    29461 atacattccg ctcggttttt ctgctatttg cataagctcg tgtaaaacac ccgccgtgat
    29521 tgatggtctt ttggtcagaa ttatcgaaca ctggtttgta agagttattt tttcaagttt
    29581 tacggcagtc ttgacacttc cttcttcaac tgcttcttca agattttcga aatcaatttt
    29641 ttcgacttcg ttatagaaat ctttttcaat attgtcttca tcaacgtctt ccgagtggtc
    29701 aatttttatt tcaatatcgg gaattattga ttttaaggta gttaaaaata tttcaagttt
    29761 ttcatctgtt atgggaagct cgggcacaac gtccaaaagt ccctccaaaa gctgttcgac
    29821 ttcgtcttcg tcatcatttt tcttcatcat cgcagtgagt gcgccggtgt tcagggacaa
    29881 aaattcatcc tgaagcatgc agtttatgtt aaccccgttt cgctccaact tgcatggcat
    29941 gtacaaaagc ggggtcaaaa attcgttttg ttttttgttt ttcgagcttt ttccgaccaa
    30001 aaacagataa ccgtatatta aatatttgtc cttttggctc agttcacttt gaatcataag
    30061 ctctgtaagc ttgctgtcag agccgtccaa acgcagatat tcgccgtaat tgtggaataa
    30121 aaattcttct tctttgagaa aaatccattt gttgtccttg tcctgtttta agtttctgaa
    30181 tgtcgcactt ttgacttctt cacgtacaca gtcgtgaaga tattggctta gttttttgat
    30241 gaaactgttg ttgaatgcag agttcaacgc ccttgttgct tcctgtagca ttttcttctt
    30301 ccttttttta atactttccc ttttttctta ttttaagcta aaatggtgct catggacatc
    30361 attaatctat atgagaatta taattcgtat tttaaagctg atttgaaggc tatttttgag
    30421 cgttgctcaa aaatagcctc aaaaaacaag gttaaaattt atcttatagg cggcttggta
    30481 agagatttaa tgcttggcag agaaaattat gatattgata ttacagttga aggcgatgca
    30541 atcaagtttg caaaaatttt agaaaaagaa atcggggcaa aaattttgag cctgcataaa
    30601 cctttcggca ccgcaaaagt tgaaattaac ggtataaaag ttgattttgc ctctacccga
    30661 agtgaacttt atcctaaaaa agggcacctg cctaaggtaa atgaaatagg atgttctttg
    30721 gaaaaagatg ttttgcgccg cgattttacg gttaattcgc ttgcgctttc tctgaatcag
    30781 caaaattttg cgcaaggact tgattatgtc ggcggattta aggatttaaa aaataaaaaa
    30841 ataagaatct tgcacgataa aagttttatt gatgatccca caaggataat cagggcacta
    30901 aaatacgctc aaaggctgag ttttgagttg gataaaaaaa cttttgaact gcagaaggaa
    30961 tatcttaaaa agattaatta tgatatgtgc tacaaaagag ttaaaaatga gttaaaacta
    31021 acatttaact caaagtctca aaatgctttt gaaacattta tcgaacaggg catttataaa
    31081 ttaatcacga aaaaaggaat cgaacttcca aaaatcaaca tcgaaaaatt aatcgccaaa
    31141 tacagaatca aacagccctg gattgtttat gtggggctta tcggggtgtt tgaggaagat
    31201 agtttttcgg ataaactgga attaaccaaa aaagaaaaag aaacaatttc agcttcaaaa
    31261 aaacttttga agttaaaaat taaaaaagat gattttggca tttataaagc ttttgagggt
    31321 aaatgcatcg aaagtctgat tatttactat ttaataagtt gtgatgaaaa agttttgcga
    31381 tatttggatg ttttgcgcgg gataaaaata aatattaaag gtcaggactt aattgctatt
    31441 ggtctaaagc cttccaaaga attttcgcag atttttgatt acgttctaag ggaaaaatta
    31501 aaaaattcta aaatcaacaa agcaaaagaa atcgagcttg ttcggggatt ttgtaactat
    31561 taattaatga accaatataa aaagcggtaa attttttgaa aaaatgttaa aataataata
    31621 tgtatatcgt taaaaatttg aaggactacc gtttaagtca taagcagagg gtgtttgcga
    31681 gctacattaa ggaaaatgta agcacgtata attgcagtga aaaaattacc aacaaagagt
    31741 atgtgagaac ttattttgcg cccgataacg actttttgat ttatagcacc gcgacagttg
    31801 acccgataag attgataacc aaaaaggctt gacaatcagg gtttagcgga taaaatgggg
    31861 atatattggg gtgtcgccaa gtggtaaggc acctggtttt ggtccaggca ttccgaggtt
    31921 cgaatccttg caccccagat aataaaaaaa gagataggaa aattttcctg tctctttttt
    31981 tatgcccgca gggcacgtca cgaacctcat ttgtgacttt gcacaaatga ggtttcgcgc
    32041 atttgatttt gcgtctgctc aaattttcgc aggcaaaatc taaattcccg atcctgagtt
    32101 ctaaacttcg gggttcaccc cctcagtaag aactcaggag aatccttgca ataaaaatct
    32161 gcgtgttgcc ttgcacccca gccaatttaa gtcccttgtt tttacttggg gcttttttta
    32221 tcctaaaatc gcctttaagt cttcttcggg tgtggttatg gtcttgattt tgaatttatc
    32281 caccaataca tttaacactg tcggagaaac aaaagccggc aaacttggac cgagtcttat
    32341 gttttcaatt cctaaatata acaatgtaag caatatacaa accgcttttt gttcatacca
    32401 ggaaagaaca agtgacagag gtaattcatt taccgagcag ttgaaagctt ttgataaagc
    32461 aatcgcaact tgaatggctg aataagcatc attacattga cccatatcaa gaagtctggg
    32521 gattcctccg atatcaccta agtcaaggtc gttgaagcga tatttaccgc aggcaagagt
    32581 taatataatt gtatcttttg gtgtttgttt gacaaattcc gtataatagt ttctgccggg
    32641 ttttgcaccg tcacatccgc ctacaaggaa gaaatgttta atagcaccgg attttacacc
    32701 ttcaattact ttgtctgcaa ccgacattac tgtgtggtgc ccgaagccta cggttaaaac
    32761 atctccgccg ttgatacccg tcattttttt gtcttctgcg tatccgccta attcaagtgc
    32821 tttttcaata actggagtaa aatccttttt atcatcaata tgaaccgttt caggatattg
    32881 aacaacggct gttgtaaaaa ccctgtcctt atagctttct tttacaggca taatgcagtt
    32941 tgtagtgaaa agaatcgcag caggaacccc gtcaaattct ttttgttggt tttgccaggc
    33001 tgtgccaaag ttacctttta aatgaggata ttttttcaac ttcggataag catgacaggg
    33061 taacatttca ccgtgagtgt atatgtttat ccctttatct ttagtctgtt ctaaaagaag
    33121 gtgtaaatct cttaaatcgt gtcccgttac aatgatgaaa ggaccttttt tgatatttgt
    33181 agttactttt gtaggttcag ggtttccgta agtttcagtg ttcgccctat ctaaaagctc
    33241 catgcatttc aggtttattt ctcctgtttt taaaaccaaa ggcaataaat catttatccc
    33301 taaatctttt gaaacagctc taagtgcttc ataaaaaaag ttgttgacgg tttcgtcttt
    33361 atagcccaaa acccgggcat gttcagcgta agcggcaata ccttttagtc cgaacaatat
    33421 caatgatttt aagctcctga tgtcttcatt gcattcccaa acgcttttga tgtcaaaatc
    33481 atttggcctt ttaatcaaat ctgaaacttt tttagtgaat atttttagtg attcatcgtc
    33541 aaagtttacg tttgtaattg tagtaaacaa accttcaatt aaagtttttg ttacttcttc
    33601 tgagggttca tttgaatttg caagttctat taacgtgctt gtcaattcat cctgcagatt
    33661 tgcagtgtct gcttttttcc cgcatacacc ttgaagggta caaccttttc cttgagctgt
    33721 ttgctcacat tgaaaacaaa acattgatgt cattt
//
LOCUS       DAOQAU010000013        32411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1293_length_32411_cov_6.695945, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000013 DAOQAU010000000
VERSION     DAOQAU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 32411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32411
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1293_length_32411_cov_6.695945"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /gene="topA"
                     /locus_tag="PKI94_03475"
     CDS             complement(<1..1336)
                     /gene="topA"
                     /locus_tag="PKI94_03475"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNW25830.1"
                     /translation="MAKAAVKKNSEEKQMKKSKSRALIIVESPAKSKTIKKILGDNFQ
                     IEASFGHIRDFPKNVLGFDVQKDFEPSFVVIPDKKKVVTKLNEVAKKCDKIYLASDPD
                     REGEAIAWHVRQILEVPESKILRIEFNEITPKAVKHAVENPRVIDMDRVRAQQTRQIL
                     DRLVGYKISPVLWEKLRNNRLSAGRVQSVALRMICEREEEIEAFVPVEYWSIMAELDK
                     SGIAVEAELSKYKDKKIEINNEQEAQKICDFLRDKNTKYEVSKIINKETTRKPSAPFI
                     TSTLQREASSKLGYGVSKTMQVAQKLYEGIELGAEGAVGLITYMRTDSVRISDDATEA
                     AKDFIVKNYGSQYYPATPNNYAKAGKKNVQDAHEAIRPSYPDRTPESVKKYLASDQYR
                     LYKLIWDRFMSSQMSNASIANTSVDISAGDYTLKVGSSKIIFDGFLKIYCEDE"
     gene            1471..2157
                     /locus_tag="PKI94_03480"
     CDS             1471..2157
                     /locus_tag="PKI94_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNW25831.1"
                     /translation="MPIPSITNLQNMQTDGAVRLYNPAAINVQELSAKTKAERHSIFT
                     HSQNNIKNHKNPVNLRTKVNVNEVSYGFYKYDEVNVSSLSRLNSSSSVMGKPEAIRKL
                     NELISEAQSKGITINLNYGFRSIETQRQLFYDQTTINGETQEQKIKDCAPPGFSEHHT
                     GYAFDMSPSRQQDYTWLINNAPKYGFEMSYPQGNRQGVSYEPWHWRYVGNQESQNVFR
                     YARVQAGIIR"
     gene            complement(2158..3246)
                     /gene="dprA"
                     /locus_tag="PKI94_03485"
     CDS             complement(2158..3246)
                     /gene="dprA"
                     /locus_tag="PKI94_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNW25832.1"
                     /translation="MTKEKYWIAFSAIEQVDSSFVQRLFNYFGDIESAFKASLNDLSN
                     IDGLSVKKAETFVRERAKINPDKALDDVVKRGLKFLTFDSKNYPYMLKNISNPPVVLY
                     YKGDLGICNLEKTLAIVGSRKASTHAKDVLTKILTEFRGTDICIVSGLASGIDTTAHQ
                     GALANNLKTIGVIASGFDFIYPTSNKVLYEKIMNGNGAIVSEYFPTFEPLPFRFPQRN
                     RIVSGLSYGTLVAEAAIKSGALITANLTLEQGRELMCIPGLISNPNTEGIYKLLKQGA
                     TMVTSAEDILSALNWQITPQEQMKIDFSKVTENERKILESIEIEPKGFDKICAETELE
                     FNTLLVCLTSLELAGIIKQIEGEKYSIK"
     gene            complement(3249..3632)
                     /locus_tag="PKI94_03490"
     CDS             complement(3249..3632)
                     /locus_tag="PKI94_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNW25833.1"
                     /translation="MDKSLEPKEWLKRALSNLNLGKSLAKGVNFEDLCFNFQQSVEKA
                     LKAILIKNKINVPRTHSISELINLLKSNSINIPDELEKTAPSLHIYAVDTRYPDNYFL
                     VDEDDYKEALEIAQKVYNWAKTIIN"
     gene            complement(3625..3933)
                     /locus_tag="PKI94_03495"
     CDS             complement(3625..3933)
                     /locus_tag="PKI94_03495"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNW25834.1"
                     /translation="MSDKTLEKIIKRILNKITAEKIILFGSRSRDDFHEDSDYDLLVI
                     CDDNVDVKKISKEIYSQFTDINKGVDVIIRPNKYIKKSLKHVNSFISSIYNEGKVIYG
                     "
     gene            4416..>5134
                     /locus_tag="PKI94_03500"
     CDS             4416..>5134
                     /locus_tag="PKI94_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25835.1"
                     /translation="MAEIPDELKTIFAKLKDEKGEAFFNKGYNQIKKFMKLEATAPEK
                     VIINANETSGFSIKGGFSPNLNSINYDNGVLKMNKAKQLDMLAHELKHSEQFSSMIRT
                     EGVGIEKLAEALAENAVKNSISESSFDFFFRGAYQNAVREGKAEEFLAAAKKNTAKTF
                     LEEFRVNYEKVLNLPKISSDSIEGIKALEQINAIKDYEGANFIGIAGDTYKNNPLEVE
                     AYAFGEKISNYFEKFMKYQKY"
     assembly_gap    5135..5234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5475..6251)
                     /locus_tag="PKI94_03505"
     CDS             complement(5475..6251)
                     /locus_tag="PKI94_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25836.1"
                     /translation="MEQKIQEAIKLFQEQKWDEAIDAFSQILETTQSAEIYNNLALCY
                     YNKSDLEKAEMFYLKALETNPKLTQVYINLADLYFRQKNFVSAINLLQSGVYELPDEI
                     VLSHYLARVYMEDARLDLAIDELNKVLEAQEENYDAYYDLGRIYFELGEYDLAVSNFE
                     SVLEYKDNNEWVYFYLGQAYEANDQVDKAMSSYLRAIALNEKFYPAYKKVAILFLARN
                     DFQDAIEYFEDYMSFDLPQEEKENIQATVERIKGVNGLNS"
     gene            complement(6260..7222)
                     /locus_tag="PKI94_03510"
     CDS             complement(6260..7222)
                     /locus_tag="PKI94_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNW25837.1"
                     /translation="MKKVLAILTLVTLLGLSAIASDLVIESKTQTFSEKDNKIKFEGD
                     VKVSIDEVKVIGQKADVTVGKNQKLDVATFYDKPYAYEVKKNKKREVKANILKVSLIN
                     KIVTAEGDTQSTVFDGKEPVVIITADSQEYNTQNNVMKARGQVIIHYKDLETFSDSAI
                     IKTNKEGDLQRIDLIGSARLKQDKHLALADHFIYNPATQELISIGHATTNAILDDGSK
                     LVLKADYQEYDQKSNTFLGSGHVKIWFKDYYAQGPKVTFYPDKKTGKPNEIYFTGRSS
                     VTQDVKTVYADKIKLILRPKNFFAEGNTKTVIKDVGSLNDNKSF"
     gene            complement(7219..7806)
                     /gene="lptC"
                     /locus_tag="PKI94_03515"
     CDS             complement(7219..7806)
                     /gene="lptC"
                     /locus_tag="PKI94_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNW25838.1"
                     /translation="MNFKNLSRKQIIYLSVFGALIAVLLWAFISAGIITHNFNRDKLI
                     GTENRQELDIAGLILTETKEGRKYWELYGETGYYNSDNKVAILENVAGNFYKDNEVTM
                     SFESSKGTYNEIKRQIILYDNTHIVIKDGTSLYTDRLVWSGSDKDVVAKGNVRINRDN
                     ELIATAQSAIITPDYATFKIAGKTVTKLYKIKEKK"
     gene            complement(8077..8541)
                     /locus_tag="PKI94_03520"
     CDS             complement(8077..8541)
                     /locus_tag="PKI94_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNW25839.1"
                     /translation="MIKDNIKNANLYYNLSKRFEIGLKYLQKNDFTNFKNGKYPILNE
                     EIFASIQDYTSKPLEEGKFEAHKKYADIQFIVKGSEKLGICNIKNFTLSQIYDEQKDI
                     EFLTPIDKSLCNFFELNEGEFVIFYPKDAHMPSLAIDKPDYVKKIVVKIQVN"
     gene            8564..8818
                     /locus_tag="PKI94_03525"
     CDS             8564..8818
                     /locus_tag="PKI94_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNW25840.1"
                     /translation="MEKKFYTYVLLTENNTLYCGYTDDVEKRFEKHLSGKGAKYTRAN
                     KPVKIIYQACFDTKSEAMKEELRIKDLTREEKLEFIKEHS"
     gene            complement(8826..10037)
                     /locus_tag="PKI94_03530"
     CDS             complement(8826..10037)
                     /locus_tag="PKI94_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25841.1"
                     /translation="MKSEKKKIFILIIFAILLNQIIFSSSVLAKKKYQNTPAQEYKYQ
                     QKVLRKLEVEKYKKSLLPQSGYMTREEYEKLSEDIPNSKIAIPSAPPPKDIKMKYIPQ
                     PTYKLIKYNNPPGTPELQIIRKFYFNRQENAQGVASPNLKMLVYPAIYYYAEAQCTAC
                     DLFVIPLDKTLPDVERVSRANVIKRNPTPILSTEKDISQKFAFRTMTPVDFSPDSSKL
                     IAKEKIGNIHDGIWKTNLWLYDFDSQSAQNLSQIRTDIENHWKKTKNLTLSEKRWDIF
                     PLGFDAQNPDMIVVAAYGYTGFRPVMLGIWGINYRDGKTSLISMNGSDAKIVTSGVKL
                     VQSGVVNPYVVKAEEKRLDKLAKKKRKSAKQEIKKEKKAAKKQLKKKLKEMKHEEKTG
                     KSLYIQQQKTP"
     gene            complement(10056..10727)
                     /locus_tag="PKI94_03535"
     CDS             complement(10056..10727)
                     /locus_tag="PKI94_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW25842.1"
                     /translation="MSNVRVLLVEDHTMTRMGLSLVLEKAQGIELIAEAEDGLQGVEM
                     AQKLLPDVILMDIGLPNIDGIEATRRIKESGIESRVLIFTSRDNEDDVFACFKAGADG
                     YIMKGASPMQTTNAILAVNEGTAWLDPAIAKIVLSNIQKGDSPSQSPINSSFKNGKNI
                     YGLTERELEVLALIVEGLSNPQIADKLVITRATAKAHVHSILQKLYVDDRTKAAVMAM
                     KEGLV"
     gene            complement(10751..12313)
                     /locus_tag="PKI94_03540"
     CDS             complement(10751..12313)
                     /locus_tag="PKI94_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW25843.1"
                     /translation="MIKSSRIVVVDDDTIVTSALKTLFKIEGFSDAHYFNNPKEAVEF
                     IKTNKPDLILSDFLMPEMNGLEFLSAAKEIYPEVSMILLTGYADKENAIKAINEIGIY
                     RYIEKPWENDDLIINIKNGIERSHLLAQLREKIEELEKAKAQLEKYSHSLEELVAEKT
                     ADLVKTNSKLSGIITYCADGIIIIDENGRIEQVNPACENFVGLSEETLKTMNFGDITY
                     SGKGQNFKEILKEHSEIFLRDYYIINSLRDKKIPVEISFATIASDEKEKVQKFVGVIR
                     DVRAQKETERLRDDFIATLTHDLRTPLLAAIQTLKFFLDGSLGNLDEKQKVLLSTMQR
                     SNEDLLGLVNALLEVYKYEAGKLNLCKTNFVLKDLINQCFSELEPLAKNKNIDFILTF
                     CAHENLTINADRGEIRRVITNLCGNAINYTNNNGKIDVLVREQNGDVIFSVADNGNGI
                     PKEDIPHLFKRFSQGTSQKRSTGTGLGLYLSRQIIEAHGGKIWLESKVSNGSEFSFLL
                     TGVKVEGKQLIK"
     gene            complement(12462..13334)
                     /locus_tag="PKI94_03545"
     CDS             complement(12462..13334)
                     /locus_tag="PKI94_03545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNW25844.1"
                     /translation="MKNIIIYSNQNKSALTEMLSSIEDSNVRVESDSLLKEYTLHNPS
                     LVIVESAPNLKEVLMTTKFFAPILIVGPIFTDVTVRAEGFDFINTPVNKDELIVRVNA
                     LLKIKELKDTLSLATTTDELTGLHNRKYLHERLESEISRAKRYKTQLSCLLFDIDFFK
                     VVNDMYGYDWGDVLLKSLADKLKFLVRKEDILTRYGDEEFLLVLPNTSEENAFLFAER
                     FRRDIEKMEFIPAGEDERHPITISGGISTYPCLENVEEDANTIIRYAEHALYNAKKRG
                     KNKIIQFSQINLDY"
     gene            complement(13405..14265)
                     /locus_tag="PKI94_03550"
     CDS             complement(13405..14265)
                     /locus_tag="PKI94_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNW25845.1"
                     /translation="MKKRLIRYSKHIYRAVIDKDFSGMAAEMGFMLVIGIFPFMLFLM
                     AIFGWMGKKSFMNPVLLFMSNIMPHDAMELIHTVLKEVMIFSKGGTMAIIGFIVTVVL
                     TSNAVAVVLKGLNTAYKVEETRSFIYTRILSTIMVFVNTFMLFLSINLIIFGKVIVNF
                     LISYAEISKAFAITLLTLRWPVAFLALYFMAFINYYILPDLKGPEKLKRASALPGTLF
                     FCMFWLAGSWGFSIYVNNLQTYNRVYGTIGAFAVLMVWMYYTSLLLLIGGEINSQVYN
                     KLEMREENNS"
     gene            complement(14262..15230)
                     /locus_tag="PKI94_03555"
     CDS             complement(14262..15230)
                     /locus_tag="PKI94_03555"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNW25846.1"
                     /translation="MEKHLEFDKALRSAKSILICSHVNPDGDTLGTMCALQLAVQKQY
                     KKKADMLILSKLPKIYEFLPNINEAKSLDKFDKSREYDVVVAIDIASLDRLIDAQILF
                     DKAKYSINIDHHTTNNLYGDLAIVEPNTSSSGEVLFKIFKKLSWEINLDIAICIYTAI
                     LTDTGGFRFENTNSSVLRVAAELVDIGVNPKELFKKCYETKTKNMVLFQNYCVGKATF
                     LNKDKIAYTIVYKKDMEKFYAGDDYTDGIAETLRSITTTDVSFVVKEVDSKTCKISMR
                     SKSSDIAKVCSVFGGGGHKFAAGCTMKYSCDDAVKKLLAEINKEVK"
     gene            15288..15614
                     /locus_tag="PKI94_03560"
     CDS             15288..15614
                     /locus_tag="PKI94_03560"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNW25847.1"
                     /translation="MAKKILYTDFEDAASIIEGMLTSKDFKKAITRNNLYKFWGKIVD
                     KKLSDKSKPYGMGQNGLMIIACQNSSVAQELMLRKTKILEDFQPYLKSLHIKVKDMKF
                     DCKKWE"
     gene            complement(15923..16111)
                     /locus_tag="PKI94_03565"
     CDS             complement(15923..16111)
                     /locus_tag="PKI94_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25848.1"
                     /translation="MNLVAYARLLHRKINSAHKKAKNPFIDASAACNPFYKPIYTTLV
                     WVEPDDEEINEFNRKQVQ"
     gene            16238..17179
                     /gene="thiL"
                     /locus_tag="PKI94_03570"
     CDS             16238..17179
                     /gene="thiL"
                     /locus_tag="PKI94_03570"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNW25849.1"
                     /translation="MKELEFISIIKKTLSKSGHIGDDCACLKDLGIVLTQDTLVEDVH
                     FLTKYTTPYLLGYKAITVNLSDIFASGAIPKYFTVSLSLPKNISPVFVEEFYKACEDL
                     SKEFNFEIVGGDITGSDKIFVSACAIGLTKGRNISSRKNAKVGDLIVTTGFHGSSRAG
                     LCLLENGKKQNKITDAHLKPELCPDFSQDISTKIADYAMMDTSDGLMDALFKIAQASN
                     VVLYADFNKIPYDKEIENIAKLANADYKDWILYGGEDYQLVACVSKDEIKKLNSKLFT
                     IIGEVKEKKETHFVEIDFQDRIEKISDLDKTFNHFGG"
     gene            17181..17834
                     /gene="ispD"
                     /locus_tag="PKI94_03575"
     CDS             17181..17834
                     /gene="ispD"
                     /locus_tag="PKI94_03575"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNW25850.1"
                     /translation="MKISVIITAGGSSVRFGKTNKLLQKINSKELIKYTVDAFMGINE
                     ITQIIICSNPSIIDELRNIFKEYKQIKIIEGGKTRQESVFNGLKACQNCDYVLIHDGA
                     RPMITKDIIKNAIRKVQEKKALTVATKTIDTIKQVDENLKIIKTLDRSSLYNTQTPQA
                     FEYELIKEAHEKLQGKDFTDDAGMLEFSGKEVYVIEGDYINIKVTTQADLEFAKTYL"
     gene            complement(17862..20840)
                     /locus_tag="PKI94_03580"
     CDS             complement(17862..20840)
                     /locus_tag="PKI94_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HNW25851.1"
                     /translation="MPQAKPLISFILPNYNNEHVIDLFFEKFIKNNTYDNYEFIVVDD
                     GSEDNGLELLYKWQNSGKINNMKVIAEPHKGIINALNKALDAVKGEFIIRMDGDATIE
                     SKSFVEKFLEFYYINPEKIGVITGRVMMDDDVLHSLGRSVISEQGLHDVGKKITSKIG
                     KRKWDQAVVIRQDLQKIMNIPAEIDTALGVCTFCDTKTAKKIGKFDENYPLWLEDDDF
                     YLTFRLHGKKCFYLPDIKICHRVSLRGNRNPDVWKNKKNFFKLIKKFIWSFEATKKGK
                     IFKILGLPLIQIKGGSENRSSNSTVCFLNIPIFKCKPIYSWREKILLHDYTYWKQKWG
                     FDPLNPDMNYIKENYKETQILWNYDKELKTEGEEILKKYSKTREKSHKIFENEKNIKI
                     LLSYHKPSVLLKNEILTPIHVGRAITKQPAKDGAFCENDLNWLFKNMIGDDTGENISD
                     LNRQFCELTAQYWAWKNQDKINNPEYIGFMHYRRHFIFNEMTAKKKRPEFDLVIYNAI
                     NENYINEIGIDTQTIENYMGNADVIAYKPLRFKKNVKKQFKDLTGEDYDLSYKFFDLI
                     LNVLYKKYPNYKKYAKKYLKGYHHFWFNAFVMKKEIFDDYCQWLFDILFEAHKKIDYP
                     KLSIKGQRVLGFLAERLHGIYLTRLIQEKKYRIKYAPLTFVKHTDLQEELKPAFNKNN
                     IAICLSSDNNYVPYLSVVIKSIIENSSAKNNYDIMILEEDVTAENKSKICNMTNEYPN
                     ISIRFYNVCAFFNDAKRYYVHGSFKLPTYYRLYAASIFKNYSKIIYLDVDTTVLSDIA
                     ELYDTNLKGFLVAATRDLGVVSRHLAGYIDKNYFKEQLMLKNPIKEYFQAGVLVINID
                     EFKKQNTEDKLIKCATASRFNYVDQDVLNKICHGQVKYLPIEWNILTEEFERIQDFKS
                     LPEEDYKYYKTGKENVKIIHHASQIKPWQDKTLDLAQYWWKYAEKTPFYKDFVSQNHH
                     FKSKKEKTNLIKKVIKKIFS"
     gene            complement(20844..22787)
                     /locus_tag="PKI94_03585"
     CDS             complement(20844..22787)
                     /locus_tag="PKI94_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HNW25852.1"
                     /translation="MKEKVKILLSYHKPSVLIKDEVLTPIHVGRSISNEASKDGAVNS
                     EDQKWLYENLIGDDTGDNISHLNRKFCELTAIYWAWKNQDKIDNPNYIGFMHYRRHLS
                     FNPKKNFKYSEFGWIDEDIIDSKYMLKHNLTPDKIKKIVSSNDCVIAKFEKRNPYQYY
                     QRCIDNRHIKDYDTAIRILKEKYPELTVYADKYNSGKKSFHYNLFVMKKNIFNEYCKV
                     LFDILFELDKQIDYENYSSMEIRAIGYIAEWITGIYLQYLIQKQKIKLLELPVILVNN
                     TDLPKTLNPAFNNKNIPIVFSLNDSIIPHLAITIKSITKNSAPDYNYDIVILYNSVND
                     ITDLNKNRINSLIKEYKNISIRYFDTEFLFQKYNPKTVYTFGGINVGTCTKFFIPIIF
                     NNYDKVLCLNSDIMVFNNIVDLYNADLENNLIGASYDMSFLSNKNRLSEYCAEVLGIK
                     SVSNYFNNRVLLFNIKKCIETDFSSECIKQAKKIVQLNNFEQNVLNIVCEDKVQYIDL
                     EWNVQYLVNDYNKKLPVKCSEKYDKCLKIPKIINYMNEQKPWINPEIDFADIWWEYAK
                     MTPFYEQIIYRNTQDYCNFKFNNLNMRLSNLENIHKPFFLTSWIITFKNNIHERFSNF
                     INIFININNKYKKKIKSKIGRRI"
     gene            complement(22808..23758)
                     /locus_tag="PKI94_03590"
     CDS             complement(22808..23758)
                     /locus_tag="PKI94_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25853.1"
                     /translation="MKRETKKTLFKKLQTLFYNLFYVKDHGDYETFHAFRIRIFKKKK
                     SQIRKIYDLLNWQKDRINLLEDANKRQEAKLNQIAYQQRILIKEPYRCVNTLLLHQKT
                     FPKYKNIHQGKSVVILACGPTLNDYKPMENVVHIGVNISFLNEKVPLDFLFCQDFFQI
                     QDYQQEIIDYKGNNCKKFFGLASHKIKRNNIPECLVTKANAERFQIYPIEQPPYFTLN
                     IDSEAFGCQHSIVFHAMQFALWTNPAKIYLVGCDCSNKGYYNGGQQPYVPPTTENGVV
                     MQFANGWQAMKEFANLYYPETEIISINPVGLKGLFQDIYM"
     gene            complement(23778..25373)
                     /locus_tag="PKI94_03595"
     CDS             complement(23778..25373)
                     /locus_tag="PKI94_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HNW25854.1"
                     /translation="MSKIRILDCTLRDGGYVNEWEFGKNAIQDIISKLIKSKVDIIEC
                     GFLTEKQYGKDFSLFNSTSALKEFINGNEKKGFFVLMLDIGDNDLNIDKLVDLKPQAI
                     SGIRLTFNPNNIDKTIKIAEKLKKMGYKIFLQPMKTLNYKDDELINLIKKVNRLKPYA
                     FYIVDSFGAMYKNDLLRLFYLVENNLHEEIKIGFHSHNNLQLSFSLAQSLMNLKTKRD
                     IIIDSSVFGMGRGAGNLATELITRYINDNIQNKYEITPLLNIYDEYLSSIFAKTPWGY
                     SLPHFLSAAHNCHPNYSTYLLNKQTISVETINILLKELSEKDKSVYNKDLIENIYLKY
                     QKHKIDDKKTRKYLSDNFKGKEILVLAPGETISSESSKIQKFIEKEKPIVISVNFSHN
                     LCDYVFISNVQRFNKLKNSNQLQEPVIITSNIAQKTKENTYILNYEKLILDDYRVSDN
                     AGLMLINFLIDVGVKNITLGGFDGFNINREKNYFDKKLISNIESEELIKKTASVKEFL
                     DKFSKTVRLEYLTTSLYAESNKI"
     gene            complement(25377..26105)
                     /gene="kdsB"
                     /locus_tag="PKI94_03600"
     CDS             complement(25377..26105)
                     /gene="kdsB"
                     /locus_tag="PKI94_03600"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNW25855.1"
                     /translation="MKIIGIIPARYQSSRFQGKPLADICGKPMIWWVYQQATKVKELQ
                     EVYVATDNKKIEEVCKKYKMNVIMTSSKHKTGTDRICEVAQKIKADLYLNIQGDEPLI
                     EPETIQKAIEPFCKNPKLQISNLMTKINDPVDIANFTIPKVLVNNENIGIYLTRAAAP
                     YPKGKIDFNYYKQVCVYGFKPKALEFFAKTKRGNLEEIEDIEILRFIENGYQVRFVEV
                     VSDTIAVDTPNDLKKVIDFIKKKA"
     gene            complement(26116..27489)
                     /locus_tag="PKI94_03605"
     CDS             complement(26116..27489)
                     /locus_tag="PKI94_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNW25856.1"
                     /translation="MVIRWKCFLGIPCIKIKEYPEYEKYYFFKLFLFKKRKSKQRMLF
                     EKVTEMSQCLNNFSTQLTHQQDRLKGVEWYFKNFESKINLLDIKTNNINDKLKIVNEK
                     IDVIKEKVLYSNEKLNSVNQKLIFNTEKNINIQNRLENIEKRLRNKEMPISTQNHERF
                     WLEKFYNEMQNFDTKEAYQNLIKNLDEESITTVNKILGRMKKLLESEAEFLEIFDKDE
                     IYAINKQRKDLHFNIIKLNEDCFAYKKYFLPKKDFESCVFYDKHELDKIKNLSKVKNK
                     NIIDAGGFIGDSALILSEYTDKNVYSFEPIKENYQYLLKTIELNKLKNVMPYNLGLGS
                     KSDKIEIFSDEARSSVNINRLETEKKETVKIITLDDFVNENKLDIGLIKVDIEGYEQE
                     FLKGAQNTIKKQKPTLLLSIYHCANDFFFIKSMIESWDLGYKFKIIKPINAQIILETV
                     LIAEVTD"
     gene            complement(27550..28527)
                     /gene="galE"
                     /locus_tag="PKI94_03610"
     CDS             complement(27550..28527)
                     /gene="galE"
                     /locus_tag="PKI94_03610"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNW25857.1"
                     /translation="MKTVLIAGGAGYIGSHTVKYMLNKGYEVVVLDNLVYGHKEAVLT
                     KNFEPVDLADKKALDAVFKKYKIDAVIHFAAYAYVGESVTEPKKYYQNNVVNTLNLLD
                     AMIENGVKNIVFSSTCATYGNPQYTPIDEKHPQNPINPYGKTKLMIEQIMQDYDRAYG
                     LKYIALRYFNAAGCDAQGELGEDHNPETHLIPLVLKAIKGEIPKITVFGTDYDTPDGT
                     CIRDYIHIEDLADAHFLAVEKLFKGGKSDCINLGTGIGTSVKEIIEVAKRVTGKDVPL
                     VYGQRREGDPPKLFASNEKAKKELGWKPKYLNVDNIIETAWLWEQNPKW"
     gene            complement(28524..29636)
                     /gene="glf"
                     /locus_tag="PKI94_03615"
     CDS             complement(28524..29636)
                     /gene="glf"
                     /locus_tag="PKI94_03615"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HNW25858.1"
                     /translation="MKKKIKNLVVGSGLSGAIIAERIASILGEEVLVIDRRNHLGGNC
                     FDYFDNNKICIHKYGPHIFHTEIEQVWQYLNNFTKFNNFEFKPQVIIDGKPCTLPFNL
                     NTLHQVFEKSKADFLEKKLVQKYGKNGKISILELKNEGDKDLKELSEFVYKKVFEGYT
                     VKQWGLKPEKIDHSVTARVPVMISCDDRYFQDKYQGIPENGYTKMIENILNHPKIEIR
                     LNVDFHQSDFEYEKLFFTGAIDEFFGYKFGKLPYRSLHFDIEEKNTEYFQNAAMVNYP
                     CDFDFTRITEHKYFLGTKSPKTVVSYEYPQEFELGKNERYYPISNPENQALYEKYLQE
                     AKKLKNVYFLGRLGDYKYYNMDLTVERALNLFKGVK"
     gene            29783..30715
                     /locus_tag="PKI94_03620"
     CDS             29783..30715
                     /locus_tag="PKI94_03620"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW25859.1"
                     /translation="MKKILVTGGAGFIGSHLCKRLLNEGNYVICLDNFFTGSKDNVKE
                     LLNNSNFKLVEHDITEEYNADVDEIYNMACPASPPHYQYDPIKTNKTSVIGILNMLEL
                     AKRNKAKILQASTSEVYGDPKVHPQTESYWGNVNPIGIRSCYDEGKRCAETFMMDYHR
                     QYGVDIRIIRIFNTYGPNMDPNDGRVVSNFIIQALKGEDITVYGDGNQTRSFCYVDDL
                     VDGIIKMMNNSQGFIGPVNLGNPSERTILDFAKMIIEMTETKSKIIHKPLPSDDPMQR
                     KPDLTVAKRELGWQPTTDIKVGIAKTIEYFSKKI"
     gene            30728..32029
                     /locus_tag="PKI94_03625"
     CDS             30728..32029
                     /locus_tag="PKI94_03625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNW25860.1"
                     /translation="MKICVIGTGYVGLVAGTCLAEMGNDVICVDKDTKKLSQLRKGIV
                     PIYEPGLEELIKVNVSEGRLVFSDKLADAVKKSLVCFIAVGTPQGDDGSADLSAVYKV
                     AEEIGKAMNEYKVIVDKSTVPVGTAEKVTEIIRNQTQHEFDVVSNPEFLKQGAAVDDF
                     LKPDRVVIGANSKRAAEIMQDLYSPFLRTGNPVIMMDVKSAEMTKYAANSFLAVKISF
                     ANQIANICEKVGADSEMVRIGMCSDSRIGSRFLFPGLGYGGSCFPKDVKAMIKTAKDF
                     NCDYKLLKAADDINKEQRTVFINKIVKRMGENLKGKTFGIWGLAFKPKTNDMREAPAV
                     TIINFLLEKGAKIVAFDPKAMETAKEYFGNKISYAKNAYEALKNADCMLLLTEWNEFR
                     RPDFDKVKELLKTPLIFDGRNQYDKKRLKARGIEYFCIGKK"
     gene            32199..>32411
                     /locus_tag="PKI94_03630"
     CDS             32199..>32411
                     /locus_tag="PKI94_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25861.1"
                     /translation="MKKKLLISVLILSAVFCVDMRAFSATKYKKVSPVAAAIKKYKVG
                     NYTGCLQDMQTIVKKDPSNAVAYYYIA"
BASE COUNT         9843 a         5426 c         5392 g        11650 t
ORIGIN      
        1 cctcatcctc gcagtaaatt ttcaaaaacc cgtcgaaaat tattttgctt gaacctactt
       61 ttaaagtgta atctcctgcg gaaatatcaa cagaagtgtt tgcaatgctt gcatttgaca
      121 tttgtgaaga cataaatctg tcccaaataa gcttatacag gcggtattga tcgcttgcaa
      181 gatatttttt aacgctttcg ggtgttctgt ccggatacga aggtctgatg gcttcgtgag
      241 catcctgaac attctttttg cctgcttttg cgtaattgtt gggagttgca gggtagtact
      301 gagagccgta atttttgacg ataaaatctt ttgcagcttc tgtagcatca tcagaaatcc
      361 ttacagaatc ggttctcata taggttataa gccctactgc gccttctgcg ccaagttcaa
      421 tcccctcata gagtttttga gcgacctgca tggtctttga gaccccgtag cccaatttcg
      481 aactggcttc acgctgtagc gttgaggtga taaaaggtgc tgaaggtttt ctggtagttt
      541 ctttgtttat gatttttgaa acttcgtatt tcgtattttt gtcgcgcaaa aaatcgcaga
      601 ttttttgcgc ttcttgttcg ttatttattt caattttttt atctttgtat ttagaaagct
      661 ctgcttcgac agctatacct gatttgtcga gctctgccat aattgaccaa tattcgacgg
      721 ggacaaaagc ttcgatttct tcttcgcgct cgcaaatcat gcgaagtgcg accgattgca
      781 ctctgcccgc agaaagcctg ttgtttctca atttttccca taaaacaggg cttattttgt
      841 aacccaccag cctatctaag atttgtcttg tttgttgggc tctgaccctg tccatatcta
      901 ttactcgagg gttttcaaca gcgtgtttga cagctttggg ggtaatttcg ttaaattcta
      961 tccttaaaat tttgctttcg ggaacttcca aaatctgtct gacatgccat gctattgctt
     1021 ctccttcgcg gtcggggtct gaggccagat atattttgtc gcattttttg gcgacttcgt
     1081 ttaactttgt aaccaccttt tttttatcgg gaattacaac aaagctgggt tcaaaatctt
     1141 tttgaacatc aaaaccaaga acattttttg gaaaatccct tatatgtccg aagcttgctt
     1201 caatctgaaa attgtcgccc aaaatctttt taatggtttt tgatttagcg ggcgactcaa
     1261 caataatcaa agctcttgac tttgattttt tcatttgttt ctcttctgaa ttttttttaa
     1321 ctgctgcttt tgccatttta tttaattttt ttgaacaaat cgtttttaaa ttatatcacg
     1381 tataatattt taagcaaata aagggcaatt tttaatataa aaatgttttt atattaatac
     1441 gaggatttta atataaatag gatttttttt atgccaatcc catctattac aaatttacaa
     1501 aatatgcaga cggatggagc tgttagactt tataacccgg ctgcaattaa tgttcaggaa
     1561 ttatctgcta aaacaaaagc cgaaaggcac tctattttta cccattcgca aaataatatt
     1621 aaaaatcata aaaatccggt taatttaaga acgaaagtta acgtaaatga agtttcatac
     1681 ggattttata aatatgatga agtgaatgtt tcaagtttat ccagattgaa ttcttcaagc
     1741 agtgttatgg gtaaacccga agcaataaga aaactcaatg aattaatatc agaagcccaa
     1801 agtaaaggca taacaattaa tttaaattat ggtttcaggt ctatagaaac acaaagacaa
     1861 cttttttatg atcagacaac aataaacggg gaaactcaag aacaaaaaat caaagactgt
     1921 gcacctccgg gttttagtga acatcataca ggctatgctt ttgatatgag cccttctcga
     1981 cagcaggatt atacttggct tataaataat gccccaaaat acggatttga aatgtcttat
     2041 cctcaaggaa atcgtcaggg tgtaagctat gaaccttggc attggaggta tgtgggaaat
     2101 caagagtcac aaaacgtttt tagatatgca agagttcagg caggtattat aaggtagtta
     2161 ttttatcgaa tatttctcgc cttcaatctg ttttattatg cctgcaagct ccaaacttgt
     2221 caagcacaca agcagagtgt taaattcaag ctctgtttcg gcgcagattt tgtcaaaccc
     2281 ttttggctct atttctattg actccaagat ttttctttca ttttccgtga ctttggaaaa
     2341 atcaattttc atctgctctt gaggtgtaat ttgccaattt agggcggata aaatatcctc
     2401 agcacttgta accattgttg cgccttgctt taataattta taaatccctt cggtattagg
     2461 gtttgaaatc aagcccggaa tgcacatcaa ctctcttccc tgctccagag tcaggtttgc
     2521 ggtgattaat gctccgcttt ttatagctgc ttctgcgaca agtgttccgt aagaaagccc
     2581 cgatacaatc ctgtttctct gcggaaatct gaaaggcaat ggctcaaatg tcggaaaata
     2641 ttcgcttacg atagcaccgt ttccgttcat tattttttcg tataaaacct tgtttgaagt
     2701 cggatagata aagtcaaatc cgcttgcgat aacacctatt gttttcaagt tattggcaag
     2761 cgcaccttga tgggctgttg tgtcaattcc tgaggcaaga cccgaaacta tacagatgtc
     2821 agttccccta aattctgtca aaatttttgt caaaacatct ttggcgtgtg tgcttgcttt
     2881 tcttgaacca actatcgcaa gagttttttc taagttgcaa attcccaaat cgcctttgta
     2941 atataaaact acaggcggat ttgaaatatt tttgagcata taggggtaat ttttgctgtc
     3001 aaaggttaaa aatttaagtc ctcttttaac gacgtcgtct aaagctttgt cggggttaat
     3061 cttagcacgt tcacgcacaa acgtctcggc ttttttgaca ctcagcccgt caatgttgga
     3121 taaatcattt aaggaggctt taaaggcact ttcaatgtcg ccaaaataat taaacagtct
     3181 ttgtacaaaa ctgctgtcca cctgctcgat tgcagaaaat gctatccagt atttttcttt
     3241 tgtcattgtc aattaattat tgttttagcc cagttataaa ctttttgggc tatttctaaa
     3301 gcttctttgt aatcatcttc atcaaccaaa aaatagttgt cgggatacct tgtatcaact
     3361 gcgtatatat gtaatgaagg tgcggttttt tcaagttcgt caggaatatt tattgagttg
     3421 ctttttagta aatttatcaa ttctgagatt gaatgagttc ttggaacatt aattttgttt
     3481 ttaatcaaaa tagcctttaa tgctttttca acggattgct gaaaattaaa acataaatct
     3541 tcaaaattaa cgcctttagc aaggcttttt cccaagttta aattacttaa agctctttta
     3601 agccattctt tcggttctaa ggatttatcc ataaataact ttgccctcat tataaattga
     3661 acttatgaaa gaattaacat gttttaagct tttttttata tatttattgg ggcggataat
     3721 aacatcaaca cccttgttta tgtcagtgaa ttgagaataa atttctttgg agattttttt
     3781 aacatctacg ttatcatcac aaataaccaa caaatcataa tcgctatctt catgaaaatc
     3841 atctctggag cgggagccaa aaagaataat tttttcggcg gtaattttat ttagaattct
     3901 tttaattatt ttttcaagcg ttttatcaga cataatatat cctttttggc aaattataac
     3961 ataaaagttt aaccaaatta ttatatcaaa gaacataatt tttgcatagt attacgaagt
     4021 gtaaatgtaa ggtttaaact gctaaaatat agtcataatt gtaaataaaa tgtaatagaa
     4081 tagaatttta tagcattttt tatacaaatt gtttttttaa tatatgtgta gtgatataaa
     4141 aaaagttgtt aattatgtat ttaattggaa atgtaaattc gggacagaat tataaagtta
     4201 attcctttgg aaaaaatgtg cttccgaaac gaaatagtgt ttcgggggat aatgcagctt
     4261 tttgtgccag aaatacaatg tcagagtcaa atgtagatcc aaagtgcact aaaaaaggat
     4321 taatagcagc aggtatagcc tttgctactg ttcttgcggg cgtagggata gtaattatct
     4381 ctcgcgggcg aaataaaaaa cttgcaaaca aagcgttggc agaaattcct gatgaattaa
     4441 aaacaatttt tgcaaaattg aaagacgaaa aaggagaggc tttttttaat aagggttata
     4501 accaaatcaa aaagtttatg aaacttgaag caactgctcc cgaaaaagtt ataataaatg
     4561 ctaatgaaac cagcggtttt tcaataaagg ggggattttc tccgaattta aattcaatta
     4621 attatgataa tggcgtttta aaaatgaata aggcaaaaca attggatatg ttggcgcatg
     4681 agttaaaaca ctctgaacaa ttcagttcaa tgataagaac agaaggagtt ggtatagaaa
     4741 aattggcaga agctttggca gaaaatgcag ttaagaattc aattagtgaa tcaagttttg
     4801 attttttctt taggggagcc tatcaaaacg ctgttagaga ggggaaggca gaagaatttt
     4861 tggctgcagc aaaaaagaat acggcaaaaa cttttttgga agagtttaga gttaattatg
     4921 aaaaagtttt aaatttacct aaaatcagtt cagattctat agaagggatt aaggctttag
     4981 agcaaataaa cgcaataaaa gattatgaag gagctaattt tataggaatt gccggtgata
     5041 cttataaaaa taatccatta gaggttgagg cttatgcgtt tggggaaaaa ataagcaatt
     5101 attttgaaaa atttatgaaa tatcaaaaat attannnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnttgggg aaaaaataag caattatttt gaaaaattta tgaaatatca
     5281 aaaatattat aagccatctg ttcaatgatt tttggattgc ttcggtggac aaaaattata
     5341 aaagccgata attcatgtct cgcaaagacg cgagattctt cgggcttcgc cctcagaatg
     5401 acgaaaaatt tttaacttat aaactcatca acttataatc ttataaacca aaaactgttc
     5461 agccattcac cttttcagct gttcagccca tttacccctt ttattctctc aacagttgct
     5521 tgaatgtttt ctttttcttc ctgcggaagg tcaaaactca tataatcttc aaagtattca
     5581 attgcgtctt gaaaatcgtt gcgcgccaaa aacagtatag caactttttt gtaagcaggg
     5641 taaaactttt cgtttaaagc gattgccctc aaataacttg acattgcctt atcgacttgg
     5701 tcgtttgcct cgtaagcttg tcccagataa aaataaaccc attcattatt gtctttgtat
     5761 tcaagaacgc tttcaaaatt tgaaaccgcc aaatcatact ctcccagttc aaaataaatc
     5821 cgccccaaat cataataggc atcgtaattt tcttcctggg cttccaaaac tttgtttaat
     5881 tcgtcaatcg ccaagtccag tcttgcatct tccatgtaaa cccttgcaag ataatgcgat
     5941 aaaacaatct cgtctggaag ttcataaacc cctgattgaa gaaggttaat tgcgcttaca
     6001 aagttttttt gtctgaaata taaatcggca aggtttatat aaacctgcgt gagtttcggg
     6061 tttgtttcta atgcttttaa ataaaacatt tcggcttttt ccaagtcgct tttattgtaa
     6121 tagcaaagtg ccaaattatt gtaaatttca gcactttggg ttgtttctaa aatttgtgaa
     6181 aaagcatcta ttgcctcatc ccatttttgt tcttgaaata atttgattgc ttcttgaatt
     6241 ttttgttcca taaaatatcc taaaaacttt tgttatcatt gagcgagccg acatctttta
     6301 tgaccgtttt tgtgttacct tcggcaaaga aatttttagg tcttaaaata agtttaattt
     6361 tatctgcgta aactgttttt acatcctgag ttacactaga acgacctgta aagtaaattt
     6421 cgttaggttt gcccgttttt ttatcgggat agaaagtgac tttcggtcct tgcgcgtaat
     6481 aatctttaaa ccaaattttt acatgaccgc ttcccagaaa agtgtttgat ttttggtcgt
     6541 attcctgata atcggctttt aagacaagtt ttgaaccgtc atctaagatg gcatttgttg
     6601 tggcgtgtcc gattgagatt aattcctgag tggcagggtt ataaatgaaa tggtctgcaa
     6661 gcgcaaggtg tttgtcctgc ttaagtcttg ccgagcctat aagatcgatt ctttgcaagt
     6721 caccttcttt gtttgttttt ataatcgcac tgtccgaaaa agtttccaaa tctttataat
     6781 ggataatgac ctgtcctctt gccttcataa cgttgttttg cgtgttgtat tcctgagaat
     6841 ctgccgttat gattacaacc ggctcttttc cgtcaaagac tgttgattgc gtgtcgcctt
     6901 ctgccgttac gattttattt atcaaagaaa cctttaaaat gtttgcttta acttcccttt
     6961 ttttgttctt tttgacttca tatgcataag gtttgtcata aaatgttgca acatcaagtt
     7021 tttggttttt tccgacggtt acatcagctt tttgtcctat aactttaact tcatcaattg
     7081 agactttaac atcgccttca aatttaattt tattgtcctt ttcggaaaat gtctgtgttt
     7141 tcgactcaat aactaaatca gaggctattg cagaaagccc caaaagagtt actaaagtta
     7201 atatagctaa aacctttttc attttttctc ctttatttta taaagcttgg tgacagtttt
     7261 gcctgcaatt ttgaaagtcg cataatcggg ggttataatt gcgctttgcg cagttgcaat
     7321 aagttcattg tctctgttta ttcttacatt tccttttgct acaacgtctt tgtcagagcc
     7381 tgaccaaaca agcctatcgg tgtaaagcga tgttccgtct ttgattacga tgtgggtgtt
     7441 gtcataaagt atgatttgtc ttttaatttc gttgtaagtt cctttgctgg attcaaagct
     7501 cattgtgact tcgttgtctt tgtagaagtt tcctgcgaca ttttcaagaa tggcaacttt
     7561 attgtcgctg ttgtaataac ctgtttcacc gtagagttcc cagtattttc tgccttcttt
     7621 tgtttcggtg agaattaagc cggcaatatc aagttcttgc ctgttttctg ttcctataag
     7681 cttatccctg ttaaaattat gagtaataat tcccgcagaa ataaacgccc ataaaagcac
     7741 tgcaatcagt gccccaaaaa cgcttaaata tattatctgt tttctgctta aattcttgaa
     7801 attcatataa attattttag cataaaaact ttattccgat gaacggtaaa cttcaaaagt
     7861 aaatttatgt aaatatattt tatatatacg ttatatataa aaaaataatg ggaatatttt
     7921 aaatgtaacc aatctctttt tctttatttt ctcctccact cctttatccg atacagtttt
     7981 tagccgtgaa aacggctatt ttattaactt cgcatttcat agtcgtgcgg cagaacgggt
     8041 caattccctt gcagggtaag tacccgtttt ttgcgtttaa tttacttgaa ttttcacaac
     8101 aatttttttc acgtaatcgg gtttgtcgat tgctaaagaa ggcatgtgcg catctttagg
     8161 gtaaaaaatt acaaattcgc cttcgttgag ttcaaaaaaa ttacacaaac ttttgtcaat
     8221 cggggttaaa aattctatat ctttttgctc gtcataaatt tgtgataagg taaaattctt
     8281 gatgttacaa atacccagtt tttcgcttcc ttttactata aactgaatgt cggcatattt
     8341 tttgtgggct tcgaattttc cttcttcgag aggttttgaa gtgtagtctt gtattgatgc
     8401 aaaaatttct tcattcaata tcgggtattt gccgttttta aaatttgtaa aatcgttttt
     8461 ttgaagatat tttaagccta tttcaaagcg ttttgaaaga ttgtaataaa gatttgcgtt
     8521 tttgatattg tcttttatca taggtttatt ttatcataaa actatggaga aaaaatttta
     8581 tacttacgtt ttgctgacag aaaataacac gctttactgc ggatataccg atgatgtgga
     8641 aaaaagattt gaaaaacatc tgtcgggcaa aggggcaaaa tacacccgcg caaataagcc
     8701 tgttaaaatc atttatcagg catgttttga tacaaaatcc gaggcgatga aagaagaatt
     8761 gaggataaaa gatttaacca gagaagaaaa gctggaattt atcaaagaac acagttaaaa
     8821 attagttaag gagtcttttg ttgttgtatg taaaggcttt ttccggtttt ttcttcgtgt
     8881 ttcatttctt tgagtttctt tttaagctgt ttttttgctg cttttttctc ttttttaatt
     8941 tcttgttttg ctgattttct tttctttttg gcaagtttgt caaggcgttt ttcttcggct
     9001 tttacaacat aagggtttac caccccactt tgaaccaatt ttaccccgct tgtgactatt
     9061 tttgcatcag agccgttcat tgagataagc gaagttttgc cgtctctgta attgattccc
     9121 caaatcccga gcataacagg tctaaatccg gtatagccgt aagccgcaac aactatcata
     9181 tccggatttt gggcatcaaa gcccagaggg aaaatatccc agcgtttttc tgaaagagtt
     9241 aaattttttg tctttttcca atgattttct atatctgttc tgatttgtga aagattttgc
     9301 gcgctttggg aatcaaaatc gtaaagccag agatttgttt tccaaattcc gtcatgaata
     9361 tttcctattt tttcctttgc aattaattta gaactgtctg gggaaaaatc aacgggagtc
     9421 attgttctga aggcaaattt ttggcttatg tctttttccg tggataaaat cggtgtagga
     9481 tttcttttta taacatttgc cctggaaacc ctttcgacat cgggaagagt tttgtccaga
     9541 ggaattacaa aaaggtcgca tgcggtgcat tgagcctctg catagtaata aattgcagga
     9601 tatacaagca tttttaaatt cggggaggca acgccctgag cattttcctg cctgttaaaa
     9661 taaaattttc ttataatctg cagttccgga gtacccggcg ggttattgta ttttatcagc
     9721 ttgtaagtgg gctgcggaat atatttcatt ttaatgtctt tgggcggcgg tgctgaagga
     9781 attgctattt ttgaattggg aatgtcttcg gaaagttttt catattcttc tcttgtcatg
     9841 tagcccgatt gaggtaaaag gctttttttg tatttttcga cttcaagctt tctcaaaact
     9901 ttttgctgat atttgtattc ctgggcagga gtgttttggt attttttctt tgccaaaacc
     9961 gaagaagaga aaataatttg gttcagcaaa atcgcaaaaa taattaatat aaatattttt
    10021 ttcttttcac ttttcacttc tcacatctca cttttctaaa ctaagccttc tttcattgcc
    10081 ataacggcgg cttttgttct gtcgtcaacg taaagctttt gcaaaatgct atgaacatgc
    10141 gctttggcgg ttgcccttgt tatgacaagt ttatctgcaa tttgtggatt ggaaagtcct
    10201 tctacgatga gcgcaagaac ttcaagttct ctttctgtaa gtccgtaaat atttttgccg
    10261 tttttaaaac ttgaattaat aggcgattgt gaaggggagt cgcctttttg aatgtttgat
    10321 aaaacaattt ttgctattgc agggtcaagc catgctgttc cttcgtttac ggcaagaata
    10381 gcgtttgttg tctgcatagg gcttgcgcct ttcataatgt agccgtcggc acctgcttta
    10441 aagcatgcaa atacgtcatc ttcgttgtcg cgtgaggtaa aaatcaaaac tctgctttca
    10501 attcctgatt ctttaatcct tcttgtagct tcaattccgt cgatattggg taagccgata
    10561 tccattaaaa tcacatcagg aagtaatttt tgagccattt caacaccctg cagtccgtct
    10621 tcggcttctg caataagctc tattccttga gctttttcaa gcactaaaga aagccccatc
    10681 cttgtcattg tatgatcttc aaccaataaa actctaacat ttgacattgt tttatccttt
    10741 aactttgtaa ttacttgatt agctgtttgc cttcaacttt aacccctgta agcaggaagg
    10801 aaaattcgct gccattgctt actttgctct ccagccaaat ttttccgcca tgggcttcta
    10861 taatctgcct tgaaaggtaa agccccaaac cggttccggt cgagcgtttt tggctggtgc
    10921 cttgcgaaaa ccttttgaaa agatgcggaa tgtcttcttt tggtatcccg tttccgttat
    10981 ctgcaacgga gaaaatcaca tctccgtttt gttctcttac aagaacatcg attttgccgt
    11041 tattgttcgt ataattaatc gcatttccgc ataggtttgt tataaccctt cttatttcgc
    11101 ccctgtcggc gtttatggtt aaattttcat gggcacaaaa agtcaaaata aaatcaatgt
    11161 ttttattttt tgcaagcggt tcaagctcgc tgaaacattg attaatcaag tcttttaaaa
    11221 caaaatttgt tttgcatagg tttagtttgc cggcttcgta tttatagact tcaagcaggg
    11281 catttaccag tccgagcaag tcttcgttgc ttctttgcat tgttgaaaga agaacttttt
    11341 gtttttcatc taagttgccc aaagaaccgt caagaaagaa tttaagggtt tgaattgccg
    11401 caagaagcgg agttcttaaa tcgtgtgtga gtgtcgcaat aaaatcatcg cgaagtcttt
    11461 cggtttcttt ttgcgccctg acgtcacgga ttactccgac aaatttttgt actttttctt
    11521 tttcgtctga tgcgattgtg gcaaaactta tttcaacggg aatttttttg tctctgagcg
    11581 aatttattat ataataatcc cttaaaaata tttctgaatg ttcttttaaa atttctttaa
    11641 agttttggcc ttttccgctg taggtgatat ctccgaaatt cattgttttt aaggtttctt
    11701 ccgacagccc cacgaaattt tcgcaggcag gatttacctg ttctattctg ccgttttcgt
    11761 caattattat tatcccgtca gcgcagtagg ttattattcc cgataatttt gaattggttt
    11821 tgactaaatc ggcagttttt tccgcaacaa gctcttccaa actgtgcgaa tatttttcca
    11881 gctgggcttt tgctttttca agttcttcaa ttttctctct taattgggca agaagatggc
    11941 ttctttcaat accgtttttg atatttatta ttaaatcgtc attttcccaa ggtttttcga
    12001 tatagcggta aatcccgatt tcgttaattg cttttatggc attttcttta tccgcatatc
    12061 ctgtaagcag tatcatactg acttcggggt aaatctcttt tgctgcagac agaaattcca
    12121 gtccgttcat ctcgggcatt aaaaagtcgg acaaaattaa atccggcttg tttgttttta
    12181 tgaattccac agcttctttc gggttgttaa aataatgtgc atcggaaaat ccctctattt
    12241 taaatagagt ttttagagct gacgttacta tggtgtcatc gtcaacaaca actatcctgc
    12301 ttgatttaat cataatttcc ctaaagactg tttcttataa taatagatta gcgcatgttt
    12361 gagaatagga caagttttta acaatattgc aatataaaaa agtttataaa taaaaaagcg
    12421 gggcgatttt aaaaaaatcg ccccgctttt tataatttta tctagtaatc cagatttatt
    12481 tgagaaaatt ggattatttt atttttgccg cgtttttttg cgttgtatag agcatgttcg
    12541 gcatatctta tgattgtatt tgcatcttct tcaacatttt caaggcaagg ataagttgaa
    12601 attccgcctg agattgtaat cgggtgcctt tcgtcttcgc ctgcgggaat aaattccatt
    12661 ttttcgatat ctcgtctgaa tctttctgca aaaaggaatg cgttttcttc agaagtattt
    12721 ggaagtacca gcaaaaattc ttcgtcgccg tatcttgtca aaatatcttc ttttcttacc
    12781 aaaaatttaa gtttatctgc cagcgattta agcaaaacat cgccccagtc gtaaccgtac
    12841 atatcgttaa ctactttgaa aaaatctata tcaaataaaa ggcaagaaag ctgggttttg
    12901 taacgttttg cgcgtgaaat ttctgattct aatctttcgt gaagatattt gcggttatgc
    12961 aaaccggtaa gttcatcggt tgttgttgct aagcttagag tgtcttttaa ttcttttatt
    13021 ttgagcaaag catttactct tacgataagt tcatctttat ttacaggtgt atttatgaag
    13081 tcaaaacctt ctgcccgtac agtaacatct gtaaagatag gtcctacaat taaaatcggc
    13141 gcgaaaaatt ttgttgtcat taaaacttct tttaaatttg gtgcagattc tacaataacc
    13201 aaactcgggt tgtgcagggt atattctttc aaaagagaat cagattctac tcttacattt
    13261 gagtcttcaa ttgaagaaag catttctgtt agagcggatt tgttttgatt agaataaata
    13321 attatatttt tcatatatct ctccttgtaa cctatcatat atattttatc agaattaatt
    13381 taaaatacaa caaaattaaa aattttaaga attgttttct tcgcgcatct caagtttatt
    13441 atagacttgg gagttaatct cgccacctat caaaagcaaa agcgaggtat aatacatcca
    13501 gaccataagc accgcaaatg ctccgattgt tccgtaaacc ctgttgtatg tttgcaggtt
    13561 attgacataa atcgaaaagc cccaagagcc tgcaagccag aacatgcaga aaaataaagt
    13621 tccaggaaga gcacttgctc ttttaagttt ttcgggacct tttaggtcgg gaaggatgta
    13681 gtaatttatg aacgccataa aatacaacgc cagaaaagca accggccaac gtaaagtcaa
    13741 aagagttatc gcaaaagctt ttgagatttc agcatagctt ataagaaaat ttactataac
    13801 tttaccgaag ataataaggt ttatgctcag aaaaagcata aaagtgttta caaagaccat
    13861 tatggtggaa agaattcttg tgtaaataaa agagcgggtt tcttctactt tataagctgt
    13921 atttaatcct ttaagaacaa ctgcaacagc atttgaagtc aacacaactg ttacaataaa
    13981 cccgattatt gccattgtgc cgcctttaga gaatatcatg acttctttta gtactgtatg
    14041 tattaattcc attgcatcat gcggcattat atttgacata aaaagcaaaa caggattcat
    14101 aaaagatttt ttgcccatcc agccaaaaat tgccattaaa aaaagcataa aagggaaaat
    14161 tcctataaca agcataaatc ccatttcagc agccattccc gaaaaatctt tgtcgataac
    14221 cgctctgtaa atgtgttttg aatatcttat gagtcttttt ttcatttaac ttccttatta
    14281 atttcagcca ataatttttt aaccgcatca tcgcaagaat atttcatcgt gcagcctgct
    14341 gcaaatttgt gaccgcctcc tccaaaaaca gagcaaacct ttgcaatatc tgaggatttg
    14401 cttcgcatag agattttgca tgtttttgaa tcaacctctt ttaccacaaa tgaaacatct
    14461 gtagtggtta tagaccgcaa ggtttcggct attccgtcgg tgtaatcatc tcctgcgtaa
    14521 aatttttcca tatctttttt gtaaactatc gtataagcaa ttttgtcttt gttcagaaaa
    14581 gttgctttgc ctacgcagta attttggaaa agcaccatgt ttttagtttt tgtttcgtag
    14641 cattttttga aaagctcttt tggatttacg cctatatcta caagttctgc ggctactctt
    14701 aatacgcttg aatttgtatt ttcaaaccta aagccgccgg tgtcggttaa aatcgctgtg
    14761 taaatacaaa ttgcaatgtc taaatttatt tcccaactta attttttgaa tattttgaac
    14821 aaaacttcgc cgcttgagct tgtgtttggc tctacaatcg ccaaatctcc gtataagttg
    14881 tttgtcgtgt ggtggtcaat gttgattgag tattttgcct tgtcaaaaag aatttgcgcg
    14941 tcaatgagcc tgtctaaaga agctatgtca atcgcaacaa caacatcgta ttctcttgat
    15001 ttatcgaatt tatcaagcga tttagcctcg ttaatattgg gtaaaaattc atatatttta
    15061 ggaagtttag acaaaataag catatcagct ttctttttat actgtttttg aacagctaac
    15121 tgcagggcac acattgttcc caatgtgtct ccgtcgggat ttacgtgtga gcagattaat
    15181 atgctttttg cactcctcag tgccttgtca aattctaaat gcttttccat tttacaatct
    15241 ttttctgcta tgataccata attttaacat aaacgaaagt ttttattatg gcaaagaaaa
    15301 ttctatacac cgactttgaa gacgcagcaa gcataattga aggtatgctc acttcaaaag
    15361 attttaaaaa ggcaattaca cgcaataatt tgtacaaatt ttggggtaaa attgtcgata
    15421 aaaaattatc tgacaagtcc aagccctatg gaatgggtca aaacggactg atgattattg
    15481 cttgtcaaaa ttcatcggtt gcgcaggagc ttatgcttcg aaaaacaaaa atccttgagg
    15541 attttcaacc ttaccttaaa tcccttcaca ttaaagtaaa agacatgaaa tttgactgca
    15601 aaaaatggga gtgagcggtg aacagtgagc agtgagggga aaggggggaa acctccaacc
    15661 acctaatgct tatagagacg tcatgctgaa ctcgattcag catctatcca acggtgtctg
    15721 ccggaaagat cctgatacta ttgaacattc taacatggag tggattattt tagcaagccg
    15781 taaggacatt ttctacggct cgttcctcgc cttgaaaaga agtcaaacaa gttcaggatg
    15841 acgtgttttt tacacccttt aattattcaa cttttcaccc ctttagcctt tcaaactttc
    15901 aaaataaaaa agcaggtgca atttattgca cctgctttct gttaaattca ttaatttctt
    15961 catcatccgg ctctacccag accagtgtcg tgtaaatcgg tttataaaaa gggttacatg
    16021 cagcactcgc atcgataaaa ggatttttcg cctttttatg agccgaattg attttcctat
    16081 gcaagagtct tgcgtatgca actaagttca ttgttgtaca ccatctttcc agagtggcta
    16141 ctacttttca ccattcttta ttccataatt attatttacc tttaaatttc attaataatc
    16201 gccgaaaagt ataatatttt tctgctaaaa taaacctatg aaagaacttg aatttataag
    16261 cataattaag aaaactttat caaaatcggg gcacataggc gatgactgtg cctgcctgaa
    16321 agacttagga attgtcttaa ctcaagacac gcttgtagaa gatgttcact ttttaacaaa
    16381 gtacactaca ccttatttac ttggctacaa agctataaca gtaaacttaa gcgacatttt
    16441 tgcgtcaggc gcaattccca aatactttac agtttcgctt tctttgccaa aaaatatttc
    16501 tcccgtcttt gtagaagagt tttataaggc atgcgaggat ttaagcaaag aatttaattt
    16561 tgaaatcgta ggcggagaca taacaggttc ggacaaaatt tttgtttcgg cttgcgctat
    16621 agggcttaca aaaggaagaa acatttcatc aagaaaaaac gcaaaagtcg gtgatttaat
    16681 tgtcacaaca ggctttcacg gctcaagccg ggctggtttg tgtttgttag aaaacggtaa
    16741 aaaacaaaat aaaatcacag atgctcatct aaaacccgaa ttgtgccctg atttttcgca
    16801 agatatttca acaaaaattg ccgattatgc gatgatggac acttctgacg ggcttatgga
    16861 tgcgcttttt aaaatcgctc aggcaagcaa tgtcgtctta tatgccgatt ttaacaaaat
    16921 cccctatgat aaagaaatcg aaaatattgc aaaacttgca aatgccgatt acaaagattg
    16981 gatactttat ggtggcgaag attaccaact tgtagcctgt gtgagcaaag acgagataaa
    17041 aaaacttaat tctaaacttt ttaccataat cggtgaagtc aaagagaaaa aagaaacaca
    17101 ttttgtggaa attgattttc aggatagaat agaaaaaata tccgatttgg acaaaacatt
    17161 caatcacttt gggggctaaa atgaaaatat ctgtaataat aacagcaggc ggaagctcag
    17221 tcagatttgg taaaaccaac aaattacttc aaaaaatcaa ttccaaagaa ttgattaaat
    17281 atacagttga tgcatttatg gggataaatg aaattacgca gataataatt tgctctaatc
    17341 cttcaataat tgatgaatta agaaatattt ttaaagagta taagcaaata aaaatcattg
    17401 aaggcggaaa aacccgtcag gaatctgttt ttaacgggtt aaaagcgtgc caaaactgtg
    17461 attatgtatt aattcatgac ggggcgcggc ctatgattac aaaagacata ataaaaaatg
    17521 ctataagaaa agtgcaagag aaaaaggctt taactgttgc tacaaaaacc attgatacaa
    17581 ttaaacaagt tgatgagaat ttaaaaataa taaaaactct tgacagaagc tctttgtata
    17641 acacccaaac tccgcaggct tttgaatacg aacttataaa agaggctcat gagaaacttc
    17701 agggcaaaga ctttaccgac gatgcaggta tgttggaatt ttcaggcaag gaagtttatg
    17761 taattgaagg ggattacata aacattaaag tcacaactca agccgattta gaatttgcaa
    17821 aaacttattt gtagctagaa caggaaaatt ccgcatactg attaggaaaa tatttttttg
    17881 ataacttttt ttatcaaatt tgttttttct tttttgcttt taaaatgatg gttttgtgaa
    17941 acaaaatctt tataaaaagg tgttttttcg gcgtatttcc accaatattg ggctaagtcc
    18001 agagttttgt cctgccaagg tttgatttga gaagcatggt gaataatctt aacgttttct
    18061 ttacccgttt tataatattt ataatcttct tcgggcagtg atttaaagtc ttgaatcctt
    18121 tcaaattctt ctgttaaaat attccattca ataggtaaat attttacttg accatgacaa
    18181 attttattta aaacatcttg atcaacgtaa ttgaatcttg aagccgttgc acatttaatc
    18241 agtttatctt ccgtattttg ttttttgaac tcatcaatat ttataaccaa aacacctgct
    18301 tgaaaatatt ctttaattgg gtttttcagc attaattgct ctttaaaata atttttatca
    18361 atataacctg ccaagtgcct tgaaacgacc cctaaatctc ttgtggcagc tacaagaaag
    18421 ccttttaaat tcgtatcata aagttctgct atatcggaaa gcaccgttgt atcaacatca
    18481 agataaatta ttttagaata atttttaaaa atggaagcag cgtaaagtct gtaataagta
    18541 ggtaatttaa aagacccatg gacatagtag cgttttgcat cattgaaaaa agcacataca
    18601 ttataaaaac gaatggatat attcggatat tcatttgtca tattgcatat tttactttta
    18661 ttttcggctg taacatcttc ttccagaatc attatatcgt aattattttt tgcacttgaa
    18721 ttttctataa ttgatttaat tacaacgcta agatagggga cataattatt atctgaagaa
    18781 aggcaaattg ctatgttgtt tttattaaat gcgggtttta attcttcttg taaatctgtg
    18841 tgtttaacaa aagtcaaggg cgcgtatttt attctgtatt ttttttcttg aataagtctt
    18901 gtaagataaa tcccgtgtaa tctttctgca agaaaaccta aaaccctttg accttttata
    18961 gataattttg ggtaatcaat ttttttatgt gcttcaaaaa gaatatcaaa aagccattga
    19021 caataatcat caaaaatttc ttttttcatt acaaatgcgt taaaccaaaa atgatgatac
    19081 ccttttaaat atttttttgc atatttttta tagtttggat attttttgta taagacattt
    19141 aatatcaaat caaaaaattt gtaggataag tcatagtctt cgcctgtcaa atccttaaac
    19201 tgttttttaa cgtttttctt gaaacgaaga ggtttataag caatcacgtc agcattgccc
    19261 atgtagtttt ctatggtttg ggtatcaatt cctatttcgt taatataatt ttcatttatg
    19321 gcgttatata tgactaaatc aaattcgggt cttttcttct ttgcggtcat ttcattaaaa
    19381 ataaaatgtc tgcgatagtg cataaaccct atatattcag gattatttat tttatcttga
    19441 tttttccagg cccagtactg agcagtcaat tcgcaaaatt gtctgtttaa atcagaaata
    19501 ttttctccgg tatcatctcc aatcatgttt ttaaagagcc aattcaaatc attttcacaa
    19561 aatgcaccat cttttgccgg ttgttttgtt attgcccttc ctacatgtat aggagttaag
    19621 atttcatttt tcaataaaac cgagggtttg tgataggaaa gaagtatttt tatatttttt
    19681 tcgttttcaa atattttgtg gcttttttct cttgtttttg aatatttttt gagaatttct
    19741 tcaccttcgg tttttaattc tttatcataa ttccagagaa tttgtgtttc tttatagttt
    19801 tcttttatat aattcatatc cggatttagc gggtcaaatc cccatttttg cttccagtaa
    19861 gtataatcgt gcagtaatat tttttctctc cagctgtaaa tgggtttgca tttaaatata
    19921 ggaatattca aaaagcaaac tgttgagttt gaacttctgt tctcacttcc gcctttaatt
    19981 tgaattagcg gaagccccaa gattttaaaa attttacctt ttttggtcgc ttcaaaagac
    20041 cagataaatt ttttgattaa tttaaaaaaa tttttcttat tcttccaaac atccggattt
    20101 cggtttcctc ttaaagaaac tctgtggcaa atttttatat caggcaggta gaagcatttt
    20161 ttgccatgga gcctgaaagt taaataaaaa tcatcatctt caagccaaag tgggtaattt
    20221 tcatcaaatt tgcctatttt ttttgcggtt ttagtgtcgc agaaagtaca aactccaagc
    20281 gcggtatcga tttctgcagg aatattcata attttttgta aatcctgtct tattacaact
    20341 gcttgatccc actttctttt tccgattttg ctggtaattt ttttgccgac gtcatgtaag
    20401 ccttgttctg aaataacact tcttcccaaa gagtgtaaaa catcatcatc catcatgact
    20461 ctgcctgtga taacccctat tttttcagga tttatgtaat aaaattccaa aaatttttca
    20521 acaaaacttt tagattctat tgtggcgtcg ccgtccattc ttattataaa ttcaccttta
    20581 acagcatcca gagctttgtt taaggcgtta attattccct tgtgaggctc tgcaatgact
    20641 ttcatattgt tgattttgcc cgaattttgc catttataaa gaagttctaa tccgttatct
    20701 tcacttccgt catctactac aataaattca taattgtcat aggtgttgtt tttaataaat
    20761 ttttcaaaaa acaagtcaat aacgtgttcg ttattgtaat taggaagtat aaatgaaatc
    20821 agtggcttag cttgaggcat atcttatatt cttcttccta ttttactttt aattttcttt
    20881 ttgtatttgt tgttgatgtt gataaaaata ttaataaaat ttgaaaaacg ttcatgaata
    20941 ttgtttttaa atgtaattat ccagcttgtt aaaaagaaag gtttgtgaat attttccaag
    21001 ttagacagtc tcatattaag attgttgaat ttaaaattgc agtagtcttg agtattccta
    21061 taaattattt gttcgtaaaa aggcgtcatt ttggcatatt cccaccaaat atctgcaaaa
    21121 tcaatttcag ggtttatcca cggcttttgt tcgttcatgt aatttattat tttaggtatc
    21181 tttaaacatt tatcatattt ttccgaacat tttacgggaa gttttttatt ataatcattc
    21241 actaaatatt gaacattcca ttccaaatca atatattgaa ctttatcttc gcaaacaata
    21301 tttaagacat tttgttcaaa gttatttaat tgaactattt tctttgcttg ttttatacat
    21361 tcgcttgaaa aatcagtctc aatacatttt tttatattaa aaagtaatac tctgttattg
    21421 aaataattag aaaccgattt gatacctaaa acctcagcgc aatattcaga taatctattt
    21481 ttgttgctca aaaaagacat atcgtatgac gcccctatta agttattttc taaatccgca
    21541 ttatataaat caacaatatt gttaaaaacc attatatctg aattaagaca taaaacttta
    21601 tcgtaattgt taaaaataat cgggataaaa aattttgtac aagtccctac attaatacct
    21661 ccaaatgtat agactgtttt gggattatat ttttgaaaca aaaattccgt atcaaaatat
    21721 cttattgaaa tatttttgta ctcttttatt aaagaattta ttctattttt atttaggtca
    21781 gtaatatcat taacactatt atacaaaata acaatatcat aattgtaatc gggagctgaa
    21841 tttttggtaa tggattttat tgttattgca agatgcggaa taatactgtc atttaaggaa
    21901 aaaactatcg gaatgttctt attgttaaat gcaggattaa gcgtttttgg taaatctgtg
    21961 ttgttaacca atatcacagg cagttcaaga agtttaattt tttgtttttg gattaagtat
    22021 tgcaagtaaa ttcctgttat ccactctgct atataaccta ttgcgcgaat ttccattgaa
    22081 ctgtaatttt cataatctat ctgtttatct agctcaaaca aaatatcaaa taaaaccttg
    22141 caatattcat tgaaaatatt ttttttcatt acaaacaaat tgtaatgaaa agattttttg
    22201 ccggaattat atttatcagc atagacagtt aattcggggt atttttcttt taatattcta
    22261 atagcggtat catagtcttt aatgtgtcga ttatcaatac acctttggta atattgataa
    22321 ggatttcttt tttcaaattt tgcaattacg caatcattag acgaaacaat tttttttatt
    22381 ttatcggggg ttaaattgtg ttttaacata tatttagagt caataatgtc ttcatctatc
    22441 caaccaaatt cactgtattt aaagtttttt ttaggattaa aagaaagatg tcttctataa
    22501 tgcataaatc ctatgtaatt ggggttatca attttatcct gatttttcca agcccaatat
    22561 atagcggtta gttcacaaaa ttttctgttt aaatgagata tattatcgcc ggtatcatcg
    22621 ccaatcaaat tttcataaag ccatttttgg tcttcggaat ttaccgctcc gtctttggat
    22681 gcttcattcg aaatagatct tccgacatgt atgggtgtta agacttcatc tttaattagt
    22741 actgaaggct tgtgatacga tagcaatatt ttaacttttt ctttcattta taaccttttt
    22801 ataaaattta catataaata tcttgaaata aaccttttaa gccgacagga ttaattgata
    22861 taatctctgt ttcaggatag tataagtttg caaattcttt cattgcctgc catccgtttg
    22921 caaattgcat tactacacca ttttctgtgg tcggtggcac ataaggttgt tgtcccccgt
    22981 tgtaataacc tttatttgaa caatcacagc ccacaagata aatttttgca ggattagtcc
    23041 aaagcgcaaa ttgcattgca tgaaaaacta ttgagtgttg gcacccaaag gcttcactat
    23101 ctatgtttaa agtaaaatag ggcggctgct ctataggata aatttgaaac ctttcagcgt
    23161 ttgctttggt aactaaacat tcaggaatat tgttcctttt tattttatga cttgccagcc
    23221 cgaaaaattt tttgcaatta tttcctttat agtcaatgat ttcttgctga taatcttgaa
    23281 tttgaaaaaa gtcttgacag aataagaaat ctaaaggaac tttttcattc aaaaatgaaa
    23341 tattaactcc tatatgtaca acattttcca ttggtttata atcatttaaa gtcggaccgc
    23401 atgccagtat tacaacgctt ttgccttgat gaatattttt atatttagga aatgtttttt
    23461 gatgtagtaa caacgtgttt acgcaacgat aaggttcttt aataagaatc ctttgctgat
    23521 atgcaatttg atttaatttg gcttcttgtc ttttatttgc atcttcaagc aagttaattc
    23581 tatccttttg ccagttgagt aaatcataaa tttttcttat ttggcttttt ttctttttga
    23641 atattcttat tctgaacgca tgaaatgttt catagtcgcc gtggtctttg acgtagaaaa
    23701 ggttataaaa taatgtttgc aattttttaa aaagggtttt ctttgtttct ctcttcattc
    23761 tttatcaaac tccgtaacta tattttatta ctttcagcat acagcgaagt cgttaaatat
    23821 tcaaggcgaa ccgtcttaga aaatttatcc aaaaattctt taacagaagc ggttttcttg
    23881 attagttctt cgctttcaat gttgcttatc aattttttat caaagtaatt tttttcacga
    23941 tttatgttaa atccgtcaaa tccacctaag gtaatgtttt taactccaac gtcaataaga
    24001 aaattaatca gcattaaccc tgcgttgtca cttacacgat aatcatctaa aatcagcttt
    24061 tcgtaattga gtatatatgt attttctttt gttttttggg caatgtttga agtaattata
    24121 accggttctt gaagttggtt actattttta agcttgttaa atctttgaac gttgcttata
    24181 aatacatagt cacataaatt atgtgaaaaa ttaacgctta tgacaatcgg tttttctttt
    24241 tcaatgaatt tttgaatttt actgctctca cttgatattg tttcacccgg tgccaaaaca
    24301 agtatttctt tgcctttaaa gttatcgctc agatattttc ttgtcttttt gtcatcaatt
    24361 ttgtgttttt gatattttaa ataaatattt tcgattaagt ctttgttata cacacttttg
    24421 tctttttcgg aaagttcttt aagcaatatg ttgattgttt caactgaaat agtttgtttg
    24481 tttaaaagat aagttgagta attaggatga caattgtggg ccgcactcaa aaagtgaggc
    24541 agagaatatc cccagggtgt tttggcaaaa attgaagata aatattcatc ataaatattt
    24601 aaaagcggag tgatttcgta tttattttga atattatcat ttatatatct tgtaataagt
    24661 tcagtggcaa ggtttcctgc acctctgccc attccaaata ctgaagaatc aattattata
    24721 tctctttttg tttttaagtt cattaatgat tgtgcaagcg aaaatgaaag ctgcagattg
    24781 ttgtgcgaat gaaaccctat cttaatttct tcgtgtaaat tgttttcaac tagataaaac
    24841 aaccttaaca aatcgttttt atacatagcg ccaaatgaat ctactatata aaaagcataa
    24901 ggtttaagtc gatttacttt ttttatcaaa tttataagtt catcatcttt gtaattaaga
    24961 gttttcatag gttgcaaaaa tattttgtag cccatttttt taagtttttc ggcaatttta
    25021 attgttttgt caatattatt tggattaaaa gttaacctta ttccgcttat tgcttgtggt
    25081 tttaaatcaa cgagtttatc aatatttaaa tcgttatctc caatatcaag cattaatacg
    25141 aaaaatcctt ttttttcatt tccgttgata aattctttca aggcagatgt tgaattaaaa
    25201 agagaaaaat cttttccgta ttgtttttct gttaaaaacc cgcattctat tatatcgact
    25261 tttgatttta taagttttga tattatatct tgaattgcgt ttttgccaaa ttcccattca
    25321 ttaacataac cgccgtctct cagtgtacaa tctaaaattc ttattttact catagtctaa
    25381 gcctttttct taataaaatc gataactttt tttaaatcgt ttggtgtgtc aaccgcaatt
    25441 gtatctgaaa caacttcaac aaatcggact tggtatccgt tttctataaa tcttaaaatt
    25501 tctatatctt caatttcttc aaggttgcct cgttttgttt tagcaaaaaa ttccaaagct
    25561 tttggcttaa atccgtaaac gcaaacctgt ttatagtagt tgaaatcaat tttaccttta
    25621 ggatacggtg ctgccgctct tgttaaatat attcctatat tttcgttgtt tactaacact
    25681 ttagggattg taaaattagc aatatccaca gggtcattaa tttttgtcat taggtttgaa
    25741 atttgtaatt ttggattttt gcaaaaaggt tcaatagctt tttgtatggt ttcaggctca
    25801 attaaaggtt catcgccttg tatgtttaaa taaaggtccg ctttaatttt ttgagcaact
    25861 tcacaaatcc tgtcagttcc tgttttatgc tttgatgaag tcattattac attcatttta
    25921 tattttttgc atacttcttc aattttttta ttatccgttg caacataaac ttcttgtaat
    25981 tctttaactt ttgtcgcttg ttgataaacc caccaaatca taggttttcc gcatatatcg
    26041 gcaagaggtt tgccttgaaa tctgcttgat tggtatcttg ccggaattat tcctattatt
    26101 ttcattttta cctctttaat ctgtaacttc tgcaattaag actgtttcta aaataatttg
    26161 agcatttata ggttttatta tcttaaattt ataccctaaa tcccaacttt caatcatgga
    26221 tttaatgaaa aagaaatcat ttgcgcaatg ataaatactt aaaagcaaag ttggtttttg
    26281 cttttttata gtattttgcg ccccttttaa aaattcttgt tcatagcctt caatatcaac
    26341 tttaatcaag ccaatgtcaa gcttattttc atttacaaag tcatcaagcg ttattatttt
    26401 gaccgtttct tttttttctg tttctaatcg gttaatattt acactgcttc ttgcctcatc
    26461 cgagaatatt tcaattttgt ctgattttga acctaatccc aaattataag gcattacgtt
    26521 ctttaattta tttagttcta ttgttttaag caaatattga tagttttctt taataggctc
    26581 aaaggaatag acatttttat cggtatattc acttaaaatt aacgcagaat caccgataaa
    26641 tcctcctgca tcaataatgt ttttgttttt aacttttgaa agatttttga ttttatccaa
    26701 ttcgtgtttg tcataaaaaa cacaagattc aaaatctttt ttaggcaaaa aatatttttt
    26761 ataagcaaag caatcttcat ttaattttat tatattaaaa tggaggtctt ttctttgttt
    26821 gtttattgca taaatttcat ctttatcgaa aatttccaga aattcagctt cgctttccaa
    26881 aagttttttc atgcgaccca aaattttatt tacggttgta atgctttctt catcaagatt
    26941 tttaattaaa ttctgatagg cttcctttgt gtcaaaattt tgcatttcat tgtaaaattt
    27001 ttcaagccaa aatctttcat gattttgggt agatatgggc atctccttat ttcgtaatct
    27061 tttttcgatg ttttcaagtc tgttttgaat attaatattt ttttcagtgt taaaaattag
    27121 tttttgatta acgctgttta gtttttcatt gctgtacaat actttttctt ttataacatc
    27181 tattttttca ttaacaattt tcaatttgtc atttatattg tttgttttta tatcaagcaa
    27241 atttattttt gattcaaaat ttttaaaata ccattcaaca ccttttaggc ggtcttgctg
    27301 atgtgttaat tgtgttgaaa aattatttaa acattggctc atttcggtta ctttttcaaa
    27361 taacatcctt tgcttgcttt ttcttttttt gaataaaaac aacttgaaaa aataatattt
    27421 ttcatattca gggtattctt taatttttat acaaggaata cctaaaaaac atttccacct
    27481 gataaccatc aaaataaact ttatccttat tcttcttata tccaaattat atcataaaaa
    27541 gctttaaaat taccatttcg ggttttgttc ccagagccat gcggtttcaa taatgttatc
    27601 aacattcaaa tacttgggtt tccaaccgag ttcttttttt gctttttcgt ttgatgcaaa
    27661 aagctttggc ggatcgcctt cgcgtctttg tccgtaaaca agcgggacgt ctttccctgt
    27721 cactcttttc gcaacttcaa taatttcttt gacagaagtt cctatgcctg tgccaaggtt
    27781 aatgcagtcg cttttgccgc ctttaaaaag cttttcgact gcaagaaagt gggcatcggc
    27841 aaggtcttca atatggatat aatctcttat gcaagtgccg tcgggggtgt cgtaatccgt
    27901 tccaaagact gtaatttttg ggatttcacc tttaatcgcc tttaaaacaa gcggaattaa
    27961 atgcgtttca gggttgtggt cttctcccaa ttctccttgt gcatcacagc ctgcggcgtt
    28021 aaagtagcga agagcaatgt atttaagccc gtatgctctg tcgtaatctt gcattatctg
    28081 ttctatcatt aattttgttt ttccgtaagg gttaatcgga ttttgcggat gtttttcgtc
    28141 tatcggagtg tattgaggat ttccgtaagt ggcgcaggtg cttgaaaaaa caatgttctt
    28201 aaccccgttt tcaatcattg cgtcaagaag attcaaggta tttacaacgt tattttggta
    28261 atattttttg ggttcggtga cgctttctcc cacgtaagcg tatgctgcaa agtggattac
    28321 cgcatcaatt ttgtactttt taaaaaccgc atcaagggct tttttatctg ccaaatcaac
    28381 cggctcgaaa tttttggtca aaactgcttc tttgtgaccg taaaccaaat tgtcaagcac
    28441 gacaacttca tagcccttat taagcatata ttttaccgta tgtgaaccga tgtatcctgc
    28501 tccgcctgcg attaaaacag tcttcatttt acccccttaa ataaatttaa tgcacgttca
    28561 accgttaaat ccatattgta atacttataa tcgcccaaac gccccaaaaa gtaaacattt
    28621 ttaagttttt ttgcttcctg taaatatttt tcgtataaag cttggttttc gggattagaa
    28681 atcgggtaat aacgttcgtt ttttcccaat tcaaattcct gcgggtactc gtaggagaca
    28741 acggtttttg gcgattttgt gcctaaaaaa tatttatgtt cggtgattct tgtaaaatca
    28801 aagtcgcaag gataattcac catagctgcg ttttgaaagt attcggtatt tttttcttca
    28861 atatcaaaat gaaggcttcg ataaggcagt ttcccgaatt tgtatccaaa aaattcatcg
    28921 attgctccgg taaaaaataa tttttcatat tcaaaatctg actgatggaa atcaacatta
    28981 aggcgaattt cgattttagg atgatttaga atattttcaa tcatttttgt atagccgttt
    29041 tcgggaatcc cctgatattt atcctgaaaa tatctgtcat cacagcttat catgacaggt
    29101 actcttgcag ttacggaatg gtcaattttt tccggtttta agccccactg cttaacggta
    29161 tagccttcaa aaactttttt atagacaaat tccgacaatt cctttaaatc tttatctcct
    29221 tcgtttttta attcaagaat agaaattttg ccattttttc catatttttg aacgagtttt
    29281 ttttcaagga aatctgcttt agatttttca aaaacctgat gcagagtatt taaattaaaa
    29341 ggcagggtgc aaggtttgcc gtcaataatt acctgaggtt taaactcaaa gttattgaac
    29401 tttgtgaagt tgtttaaata ttgccaaacc tgttcgattt ctgtgtggaa aatatgagga
    29461 ccgtatttgt gtatgcaaat tttgttattg tcaaagtaat caaagcagtt tccgccgaga
    29521 tggtttctgc ggtcaattac caaaacctct tcgcctaaaa ttgaggctat tctttctgca
    29581 ataatagcac ccgaaagccc tgaaccaacg acaagatttt taattttctt tttcataaaa
    29641 ctctctgaat atgcaagcaa taatataaca taaaaatttt ttcatgttaa aatgttaagg
    29701 ggtaaatata ttttagactt ttttattcac tcggtggatg agggataaag agaagttaaa
    29761 agaaagaggg aatctatagc aaatgaaaaa aattcttgtt acaggcggtg cgggttttat
    29821 aggaagccat ttgtgcaaaa ggttgcttaa cgaaggcaat tatgtaatct gcctagataa
    29881 tttttttaca ggttcaaagg ataatgttaa ggaattgctt aataactcta attttaaact
    29941 tgtcgaacac gatataacag aagaatacaa tgcagatgtt gacgaaattt ataatatggc
    30001 atgtcctgca agcccgcctc attatcagta cgacccgatt aagacaaaca aaacatccgt
    30061 aatcggcatt ttaaatatgc ttgaacttgc aaaacgcaat aaagcaaaga ttttgcaggc
    30121 atctacaagt gaagtttacg gcgatcctaa agttcatccg cagacagaaa gttattgggg
    30181 aaatgttaat cctatcggca tcagaagctg ttatgacgaa ggaaaacgtt gcgcggaaac
    30241 ttttatgatg gactatcacc gccaatacgg tgttgatata agaattataa ggatttttaa
    30301 cacgtacgga ccgaatatgg atccaaatga cggcagggta gtttctaatt tcataattca
    30361 ggcactaaaa ggtgaagata taaccgttta cggcgacgga aatcaaacac gttctttctg
    30421 ttacgttgat gatttggttg atggaataat taaaatgatg aacaattcac agggctttat
    30481 cggacctgta aatttaggta atccgagcga aagaacgatt ttggattttg caaaaatgat
    30541 tattgaaatg actgaaacaa agtcaaaaat tattcataaa cctttgccaa gtgacgatcc
    30601 gatgcaaaga aaaccggatt taaccgttgc aaaaagagaa ttaggctggc aaccgacaac
    30661 agatattaaa gtcggtattg ccaaaacaat agagtatttc agtaaaaaga tttagcagga
    30721 gaaagtgatg aaaatttgtg taataggaac gggctatgta ggtttggttg caggaacttg
    30781 tcttgcggaa atgggaaatg atgtaatttg tgtcgataaa gacacaaaaa agctttctca
    30841 acttagaaaa ggaatagtac cgatttatga acccggattg gaagaattaa taaaagtaaa
    30901 tgtaagcgag ggccgtttgg tttttagcga caaattagca gatgcggtta aaaaatcttt
    30961 ggtatgtttt attgcggttg gaacccctca gggggatgat ggttctgctg atttatcagc
    31021 tgtttacaaa gtagcagaag aaatcgggaa agcaatgaac gaatataaag ttattgttga
    31081 caaatcaact gttcctgttg gtacggcgga aaaagtcaca gaaattataa gaaatcaaac
    31141 gcaacacgag tttgacgttg tttctaaccc tgaatttttg aaacagggtg cggcggttga
    31201 tgatttttta aagcctgata gagttgtaat tggcgcaaac tcaaaaagag cagccgaaat
    31261 tatgcaggat ttgtattctc catttttgag aacaggaaac cccgttatta tgatggatgt
    31321 taaatcggcg gaaatgacaa aatacgcggc aaattctttt cttgcggtta aaatttcttt
    31381 tgcaaaccaa attgcaaaca tctgtgaaaa agtcggcgcg gactctgaaa tggtaagaat
    31441 aggtatgtgt tctgacagca gaatcggttc aagattttta tttccgggct tgggctatgg
    31501 tggaagctgt ttccctaaag atgtaaaggc aatgattaaa acagcaaaag attttaattg
    31561 cgactacaaa cttttaaaag cagccgatga tattaacaaa gagcaaagaa cagtctttat
    31621 aaacaaaatc gtaaaacgta tgggcgaaaa ccttaaaggc aaaacttttg gcatttgggg
    31681 gcttgctttt aagcccaaaa caaacgatat gagagaagca cctgccgtta caataataaa
    31741 ctttttactt gaaaaaggtg caaaaattgt tgcttttgat cctaaagcaa tggaaaccgc
    31801 aaaagaatat ttcggcaaca aaatttctta tgcaaaaaat gcttacgaag ctcttaaaaa
    31861 tgcagattgt atgcttctat tgacagagtg gaatgaattt agaagacccg attttgataa
    31921 agttaaagaa ctgcttaaaa cacctttgat ttttgacggt agaaatcagt acgacaaaaa
    31981 gcgtcttaaa gcaagaggaa ttgagtattt ttgtataggt aaaaagtaat tttaaggggc
    32041 gaaaattttt gaaaaaattt tcgccccttt ttatcaattt cctctcaaaa atcatctgtt
    32101 tagtgcagag gattagcggc aaaatgattt ataattaaat acagatgagt tgatttattc
    32161 aaaaaaatga ttaaaatagg ttaaagggaa gcaagtcaat gaaaaagaaa ttattaatat
    32221 ctgttttaat attatcggcg gtattttgtg ttgatatgcg tgcgttttcg gcaacaaaat
    32281 acaaaaaagt atcgcctgtt gcggctgcaa taaaaaaata taaagtcggc aattatacgg
    32341 gttgcctgca agatatgcag acaattgtca agaaagatcc gtcaaatgcg gttgcttatt
    32401 attatattgc a
//
LOCUS       DAOQAU010000014        29139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1593_length_29139_cov_6.499862, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000014 DAOQAU010000000
VERSION     DAOQAU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 29139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29139
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_29139_cov_6.499862"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKI94_03635"
     CDS             <1..237
                     /locus_tag="PKI94_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25862.1"
                     /translation="LQGQGEEVEEEDEDATEELHEKADRKFEEEKAKLNSQETILDLE
                     QNDLETQHTALAADIDSVKKLIADAIKIFKVFEG"
     gene            complement(406..1242)
                     /locus_tag="PKI94_03640"
     CDS             complement(406..1242)
                     /locus_tag="PKI94_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNW25863.1"
                     /translation="MNDSFITALNTQKATTQWMDVLAENLSNVYTPGFKENKVSFSTF
                     LGGVVSDNYNKNLSQGKSTPGTANSNLFLEGQGYFLTRTAEGQSIYTRHGDFTFDKEG
                     VYKNKDGYTVQGYILNDKGEIMQGIKSVDADLYQQTALKGGSMNIPTTNIKLWIDPNN
                     GKYLGKYDDYEIKGDGTVYGKAEGGKRTVPLYRIATMNFHNPQGLYEIKQGQYVATEE
                     SGKPVIGRGEIRSGLLELSNTGFKDNIAYFQQAKLQLELSNKLIQSQKQLLEEALKLV
                     GS"
     gene            complement(1235..>2028)
                     /locus_tag="PKI94_03645"
     CDS             complement(1235..>2028)
                     /locus_tag="PKI94_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25864.1"
                     /translation="NLKMKNEQKKDFKTEGEPKEIISQNIQSNTVLKANNKQEITVEV
                     QTQVKFNPEIKSAKKLIKKDEALNIKKETFVKTINEVAKTDKVERFINVKNESKDFTH
                     AKVKKDSKAKLEVVVDYKTVKMEVNDGLFFVNLLKNNIAYFQAIGKEVLIEADEKEVE
                     RSIQVSATLMDALQKAVKTNKPFRIDFDKDISVILKIDKQGKISAEFIPGDKAVEEYL
                     KLNLPMLKQSFNQQNLPYENLSYRRHNQQQQQNNNNQKNKEKDNE"
     assembly_gap    2029..2128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2129..2962)
                     /locus_tag="PKI94_03650"
     CDS             complement(<2129..2962)
                     /locus_tag="PKI94_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25865.1"
                     /translation="MNIDTPKIKKENKINPNNISMQSINFLPHIETSSFKNEINAISE
                     IKNDKKNVELKAEERINQFINQAVDESIKTVNKNFSMPVVAQNEALKCPSPLNVVQNS
                     KIETAKPVQTKAEVKPEMLKPVETKPLVDTVKEILPQGVEPKNEIKFEQKVEPETEPK
                     NKIKSEMKSFDFEVITPVKIKPEMQTETKSVVDNQIKPVVKAVVKQEEKQVINKIEEK
                     QTAGIVKDIMPDFVKTDEAKPVEKKVEAKIETKIETAKPVQIKAEVKPEMLKPVETKP
                     LV"
     gene            complement(2952..3455)
                     /locus_tag="PKI94_03655"
     CDS             complement(2952..3455)
                     /locus_tag="PKI94_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25866.1"
                     /translation="MPFVYRLQKILDFRIRKKEEQLLAVQKAQRVLAIAEENIRRNDA
                     EIEEIKVNQRKADALLMEAFDKYLKHLWEKAQMLEQIRQDKEQKLREEIELLVECEKA
                     VKVLEKHKERAKEAYLDEQKKAELKQLSEVGVQRHFQQKKQKDEEEEKELLRQIEQIE
                     RETLNEY"
     gene            complement(3617..5107)
                     /locus_tag="PKI94_03660"
     CDS             complement(3617..5107)
                     /locus_tag="PKI94_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNW25867.1"
                     /translation="MVNSISQTPKTTKASIFYINDIHGQVTKMERIKTASDQFDSFTP
                     QSKTDKLKLSSGDTMLGSGVKENKAIAEFLKVIGITATAVGNHECDIPPEMLIELTKN
                     HNYKLLGLNANIVDNNPWNGKILKSCIQEINGNKYGIMGIMPSDIFLRIKNQENFAGI
                     TVDEWDQTIKEVQQEIDNLKQQGINKIILLSHGGYELEKRIAKETSGIDVIIGGHSHD
                     LLKGVEEGKNLLYSNKTGEPVVITQAGKDGNNFGILNLEFNENGVITQVQNNVQETKS
                     FAKNAPMQYFFDTIIGKSEKVGFISKAYDKELNRLAEENPNASFLADAIKSELKTDIA
                     LINASNMRGEFVVGDVYTRDITSLTPFKDKMTIVELTEKEIVDALKFGAKSLTKPDFK
                     PGIMQVAGIRYKINKSGELLEAKIIDRYNKETIVDINNPSTDKKYTVGMHDFLTRGGD
                     GYSMLDKRNSALARFDFDKDKCAIDYIKKAASPIEIQLDGRIQIVD"
     gene            5267..6535
                     /locus_tag="PKI94_03665"
     CDS             5267..6535
                     /locus_tag="PKI94_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW25868.1"
                     /translation="MQNNSYRNAIKFLPTLFDNKIQMDTSAEALFVAIKRIINFDDAY
                     IFFLNPESIQLKYIYGKHSILKPEQTFFVNDRLKRELFSEKSQITDKNSHLISALKVL
                     HYNSFLVSKLAIRNTVYGFVLLCKKENKFYSTQDVEITESVSAVISYMIKDLELSNVF
                     KMQLKALKDGIIQTDRAYKTIKEQNLKILEADKIKNEFLANISHELRTPLNAIIGFSE
                     VLGAKLFGDLNEKQEEYIKDIHVSGLHLLGMINEILDISKIEAHAMSLNRTKFMISMA
                     VNEVVNVIRPLADKKKIIIEKTIKNDGEFFADFQKISQILYNLLSNAIKFSNEGGKVR
                     IYVDLTSKELILKVEDNGIGIEEKFHGKIFAKFVQLESSYTKKESSTGLGLTITKELV
                     EMHGGEISLKSEINKGSTFMAKIPVRSDCG"
     gene            6528..6884
                     /locus_tag="PKI94_03670"
     CDS             6528..6884
                     /locus_tag="PKI94_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW25869.1"
                     /translation="MAKILIAEDNDLNLKLMYDILLTQGHEVDCATDGQMALELLLSR
                     KYDLLLLDLQMPKLSGFDVMKEILKQKIQIKTIVVSACVMSDEINRAKDLGCEEFIVK
                     PIRLHEFLSTINRVLG"
     gene            complement(6957..7955)
                     /gene="dusB"
                     /locus_tag="PKI94_03675"
     CDS             complement(6957..7955)
                     /gene="dusB"
                     /locus_tag="PKI94_03675"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNW25870.1"
                     /translation="MNEIKIGNLTLKSQFSLAPMAGITDYVLRNLIRKYSKNSLLTTE
                     MISSEALIQIQEADILKKDENHSPIAYQLSGHKPELMAKAAKILEKYADIIDINMGCP
                     VNKVVKGQDGCALMRNPKLAADIVKEVKSAINKPLSVKFRLGYTLDEINFVDFGVKMQ
                     AAGADFITIHARTRSQMYSGKADWTKIKELKQSVDIPVFANGDVNSIESAIECLEISG
                     ADGIALGRGVLGDVTLISRIEHYLNTGKKLSEPSLEEKIQALKEHLNGEIEFRGKDVG
                     IKFVRKFYPYYISGIQNAAKYRGALVVEDDYKKIIKMLDEILLSGLYPYRRLSRSR"
     gene            complement(7948..10059)
                     /locus_tag="PKI94_03680"
     CDS             complement(7948..10059)
                     /locus_tag="PKI94_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25871.1"
                     /translation="MKKLFLLIFCLFLSFQSVRADFAQHYELGQQYLSQYQYSGAISE
                     FRNALRINYLDNSARVGLVNSYLARGTYYANNEKDWNAAANDYRAALFYLKYYASQQD
                     TDNSTAAIANATDNLNTCLAAAKFSTIPQSRFQKAKELRENGNFAEAGYEFAQTLTDP
                     VLRKSALEQLADIMKILGNDPKAADYYQKAVAIDDTNPGLRLKYARVLDRLGQNDVAV
                     QEYNFALSRCNDDPEILFALERIYRQKLQQTPNDAEVMANLGAILQKQNKFDEALQYY
                     SQSAQLNPSNVTTRINVGTLYQQKKNYDAAIAAYDTVLTLYPDNKDANLFKAQCLAAK
                     GATQDAIVQYKRVLSMDPTNQDIKNQIVELLKTTMTPAEMLAYLQKDLMTDSGALDTM
                     YSYAIELHKQKKYDDAVLFYQEVLKSRVNNPEIYVNLAICYRQKNDLNNALQVLQNAK
                     NRFPTNKQIIDNIASLQEEESSTKIEEAYEYYKNNDFQKALAAYSAIQPATFDSLSGI
                     AICYKSLNNNDKAIEYYKKAFDLAPANSDVAYYIGVLYSEKEDWATAKLYLKKALSIN
                     KDNTKAQDLYQSVVEQANIKLMDTAIGYYEKADYVNALRLINQILTDDAKNAYAYYYR
                     GLISDEQKKYAIALADYKRAIQYSSELTVVYYLMALDYDALMQYKNALLNYKKYVSLT
                     PEQNEYKNYSQARIKELKQYE"
     gene            10430..10819
                     /locus_tag="PKI94_03685"
     CDS             10430..10819
                     /locus_tag="PKI94_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25872.1"
                     /translation="MAAGIQALLGFLIPERNRCTSELMGITDTIYDATERTLELNEKY
                     EEQKDEIEYALDENPNNQEALAAREELREQYYCQLSDLKDFQKQLETKKTKLDGESTF
                     LSASITSYQKMLSDDTKKNFTYGGSGG"
     gene            10900..11877
                     /locus_tag="PKI94_03690"
     CDS             10900..11877
                     /locus_tag="PKI94_03690"
                     /inference="COORDINATES: protein motif:HMM:NF023233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3326 domain-containing protein"
                     /protein_id="HNW25873.1"
                     /translation="MGRKKKKLGAFVVPTGVGASIGGFAGDASFWAREFSKHCNLIVN
                     PNVVNAGCFSGITENMLYVEGYTLDEFFKGKISLIPCQNNKIGVVFDKSISQEVLNIH
                     INTINAVKTVYGLDILGYEITEENVGVDFFVDKSNISTGSVKNLKTLLNSARKLIKKG
                     ANAIAIVCRFKDDKINENYQNGIGVDPIGGVEAIISHYISRELKIPAAHSPAFDDFSI
                     STEVVDARASAEYITPTFLPCVLLGLANAPQICKFSPKKLNINDLDFLIMPYNCLGSI
                     PVFESIKRKIKIYAIKENSTLLNFKKEILFKNSDIIEIENYKKCLELIK"
     gene            11901..12602
                     /locus_tag="PKI94_03695"
     CDS             11901..12602
                     /locus_tag="PKI94_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25874.1"
                     /translation="MTLAELTVMFVIMAVVIMATLGISKTKTDKIKAYQYYAAFTNLK
                     QAVGEVFADGYRETPESPIEKQLTPYGHTGENTGICDRLAEVFNTIGKVDCTISGGDN
                     FSDENVNFTLTNGERFFNIGKDIDGEAFRLYIDIDGKKGKSVLDEDVMEFRIYRNGTV
                     LPGETSIGGNNKNYLSASIKEINTNTWDQKDIPYLKAVCNAGEMPESYCTPLGIPLKE
                     NCTSPNVCQVYINKP"
     gene            complement(12604..13326)
                     /gene="bioD"
                     /locus_tag="PKI94_03700"
     CDS             complement(12604..13326)
                     /gene="bioD"
                     /locus_tag="PKI94_03700"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNW25875.1"
                     /translation="MLNLFITGTDTGVGKTFITAGLAATMQSLGYTTCVYKPIQCGAK
                     EKQGFMQAPDLAYIKNIDPYVKTYSTYMLKEKAEPLVAAELEGIKIDKTVIKKDFDNI
                     AKSCDCLLTEGAGGIMTPLAQNFTMNNLIKFLNLPVLVVIKPDFGTVNQTLLTINQAI
                     SSGIEVRGVIINNFPENTKDTAIKTIPRLVEEYTDTKILGIIKHFSDIDKINPNDLIT
                     TIINGIDIESVFKVKIAKLELE"
     gene            complement(13349..14215)
                     /locus_tag="PKI94_03705"
     CDS             complement(13349..14215)
                     /locus_tag="PKI94_03705"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HNW25876.1"
                     /translation="MSMQVLSEYLEFLEVEKGLSLNTIDAYRRDLSDFLDFCFALDVF
                     DLDKIQRTHINSYIRNLREKNYSSTSVMRKIASLRNFFKWLCANEICPQNPTLTLEQP
                     KVPKRLPKVMSVQEIEEILRNGLNPTEKVIVELLYGCGLRVSELVGLKTNSFDLNANY
                     LKCSGKGSKERLVPLGKKAKDAIREYIEKRDFLVKKNGLDTKNFLISDNGRFLTRQDI
                     YNFIHKQGEKIHKNISPHTLRHSFATHLLENGADLRIVQELLGHSDVSTTQLYTHISK
                     KRLKEVYFSINK"
     gene            14335..14568
                     /locus_tag="PKI94_03710"
     CDS             14335..14568
                     /locus_tag="PKI94_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25877.1"
                     /translation="MNVNKINPFSTQAMKGLQGINPSQQPVNAVKPVGKNPFEKHDQK
                     SKYGVGLINSDLNNMYTKAPNGKEGLANTRWIA"
     gene            complement(15070..15324)
                     /gene="rpmA"
                     /locus_tag="PKI94_03715"
     CDS             complement(15070..15324)
                     /gene="rpmA"
                     /locus_tag="PKI94_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNW25878.1"
                     /translation="MAHKKGMGSTRNGRDSQAQRLGVKKYGGEAVKAGNILVRQRGTK
                     IHPGINVGIGTDDTLFALIDGVVKFEPHRRDRKQVSVYAG"
     gene            complement(15365..15715)
                     /gene="rplU"
                     /locus_tag="PKI94_03720"
     CDS             complement(15365..15715)
                     /gene="rplU"
                     /locus_tag="PKI94_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNW25879.1"
                     /translation="MYAIVETGGKQYQMEEGRYLKVELLDGEKDSKVVFDKVVMLANG
                     KKSRVGQPYISGASVEGKILEHGKEKKVLVYKQRPKKGYRNKQGHRQGFTKVMITKIR
                     TSAQKKDSEGASEE"
     gene            complement(16038..19166)
                     /locus_tag="PKI94_03725"
     CDS             complement(16038..19166)
                     /locus_tag="PKI94_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNW25880.1"
                     /translation="MQTHLNNFEYPKGFDFERAEKIIRNWQKALRDGNYDETKETRVQ
                     ASFLNLFFNIILGYSEMHDNPDEWYLINEAKTEFDGTKADGALGYFTKDEKLNVTRAV
                     IELKDAKTPLDKKQSTRKDYDSPVSQAFSYLFKFDRCDWIIVSNFKEIRLYHKSRGQG
                     YYEEFKILDLHDPKEFKRFYFLLCKQNLLDKDRNSLLDNLIKDTSKNEEEITKDFYKD
                     FKELRLELFKHLKENNPEIGAKLILEKTQKLLDRMVFIFFCEDTNNLLPSGIVKQVYD
                     LGIKSRERSDQRIWREFKNLFMDIDEGRHDVSPPINAYNGGLFSSDIELENLVIKDDI
                     WQKITKLSDYDFESDLNVNILGHIFEQSLSDLEQIKAEIDGVEEDKTKSKRKKDGIFY
                     TPEYITRYIVEQTVGKYLEENPDKLKTIKILDPACGSGAFLNQAHSFLREQYQIRTEE
                     KIDAERSSNRGGQEFLQTNLLKHTNIAETDRSILLNNIFGVDLNEESTEITKLALWLK
                     TARKDKPLENLDNNIKCGNSLIDDEAIAENKAFKWNEQFKEILDGGGFDCIIGNPPYF
                     NIDTFGFGSPVFEHLKNSYSDVYMDKSDILFYFIKKAIDLLKANGIMGFIISNAFLFS
                     DKAKKLRNYILEQCSILEIVNFEQYYVFKDAGITSCILILQKNGNKEKTRAFSFEEKQ
                     YTEDIILNTINTKSNFFDVEFQLDMPFALVNSKISSINDKIDSKYPKLDEITHIGKGM
                     ETAADNIFIFDKYPNEFPSEFIKKRVSGKNIDKYFIDSNTEYILYFEDIDKFEQLPLS
                     IQNHLISNKEFLENRATVKNEGRTWWRYSRPMHKEYYNKAKLFCSRRSFSNVFCIDEK
                     FEYLSFSNMTVIFDTNKDYNIKYILALLNSKLLTYRYRSIGKQTGGGSFEYFPNGVGQ
                     LPIAKATQEQQDNLTSKAEQMIELNKQLHEGVKSALELLETEYKPKKISQNLERFYTL
                     GLHPFIEELEKQGVKLSLSQKEDLMNWYNQKSETLNSIKNQIDTLDHAIDQEVYKLYN
                     LTEDEIKIIEEG"
     gene            complement(19305..22481)
                     /locus_tag="PKI94_03730"
     CDS             complement(19305..22481)
                     /locus_tag="PKI94_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW25881.1"
                     /translation="MSSDEFNYRYIKDKATPGSWRRGYEYHLKDQVLEGYPEKNFYKG
                     KVKGNFQDFYKTDLIFKKGGVEARCNCPLKEEWCKHAVAVGLKAIDEHAFEDFLETKY
                     GIESSFEDENTALTEPPKGNYIFHFNPKRKPNFFSILVMSRETGKVVRSIEQILKGLI
                     EAQKQDKNFELNTSQMVEVAIFKQLLKTSRQDKKAGWYDIQLTKFADMFPLLAKADEV
                     LDEKSKQRLKFTDKEWRLALSVNYSQAGNIMLALEWKRPDKDDVYPFEEIRYFSRHLK
                     WGRYKNVIFPINIEVGAIPQYLVKSSFTDLKDSDGGKFIYEEMPGLQKIMDVEVSEEI
                     NKLMLEQKPPLNIVTMGIDYDQSLKASLEFEYDGIRVPFSKTSEKTPYVTVKKPKEDS
                     QYWIKRNLKHEQDAYNMLLACKFVPMQTNNLALEKENAVDFYNYYIKQAGEGWKFEEK
                     DDLGFFKLAEDSFKLRAEVDFCEESKDSFQIYLYGQVGEDKISFDDVYNTIQSGEKYA
                     RVRSLGFVEYPAQDIYAMMRSFNSFDVYRNNEDKFLVKTYRAGLLNELENFGVELDLS
                     EDFSNFWDQISSFSTKEDIEIPKDVKAELREYQAKGFSWLWFMYKYGLNGILADDMGL
                     GKTLQALTLLQRAKENDGPMPTLVICPTSVVFNWELEIQKFVPGLTCLKLAGIERKEL
                     FKDIPKYDIVITSYALVRRDIEKLKKINFRYVILDESQNIKNALSQTAQSVKRLVCDH
                     KLALSGTPIENRLEELWSVFDFLMPGFLFSISEFNYRYVNPIMERADKTVEKRLKLQI
                     YPFILRRMKRDVAKDLPDKVENIAYCELTDEQRDFYLEVLDSTKEELFKSIEQNGLEK
                     SRMSIFSALLRLRQICCHPRLYDKENIKKINESGKFEHLKEMLEEIISEKHRVLLFSQ
                     FVDMLELIREWLDKEGIRYEYLTGQTKDRQGAVERFNNSEDIPIFLVSLKAGGTGLNL
                     TGADYVIHYDPWWNPAVEDQATDRAYRIGQKKKVFVYRLITKNTVEEKIQKLKQVKRN
                     LVDSVISVDRNITKSLTMDDVREIFSVD"
     gene            22835..24088
                     /locus_tag="PKI94_03735"
     CDS             22835..24088
                     /locus_tag="PKI94_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNW25882.1"
                     /translation="MPEMARNYQYQQYRQYQNPAYGQRPAYGYRQTYPQVVTPVRPVA
                     QPIRKKVVRKRKVNPIARIISLSLLALIGYYVLPFGFQRISTPLISLSGNSYKEIQFN
                     KYQLLNTKREKIPYTYEILKPTTNYLSNDSFMNLRMLSTAKAKKPDMTTLYMTDKMQV
                     VEAKLKRLMSEYKSIEPSIFVWEYDSGKFVDIDAEKTFPAASIIKIPVLIRLFKSIEA
                     NQLTIYDEMAMTDYFKAEGSGNLQSWGIGKKYTIDQLARYMITESDNSATNMIMSKIG
                     SMQDVNQGIRSWGINSTRVQTWLPDMDGTNYTTAKDLATMLYNLDNPGFLNINSREYI
                     IDYMSHVENNRLIQAGLDPKALFVHKTGDIGKTLGDAGIVFAPNGKKYICVILANRPY
                     NSPLGKDFIQKASNIIYTSITSGVY"
     gene            complement(24090..24533)
                     /locus_tag="PKI94_03740"
     CDS             complement(24090..24533)
                     /locus_tag="PKI94_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25883.1"
                     /translation="MTVRVLILLLFTPFLGRACALGASAPVVLQAQQTLAAAQAVQPQ
                     AVDFGLCRKIFKIDAQKLFYLTLAAVNANRFTIDEIQSKSGYILFSVAKKQFLANVIS
                     VNAENSMLKITPCDNIYFFPVGIVQNMFKYVELNLNTPIEKLNVL"
     gene            complement(24764..27727)
                     /gene="polA"
                     /locus_tag="PKI94_03745"
     CDS             complement(24764..27727)
                     /gene="polA"
                     /locus_tag="PKI94_03745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNW25884.1"
                     /translation="MSNQKTLILIDGHALAFRMYFALERTGMKTTDNQPTWAVFGFFK
                     AVFDMLKDQNIKPDAITVAFDVGRKTFRVEKYDQYKANREAMPDTLRSQMSLIFEGLK
                     AFNIPIYTKEGYEADDVIGTICDKATKLGHKTLILTGDQDSFQLIDKEGNVKVLIPSK
                     GELIEYDWNKVYEKLGVYPDQVTDYKGLRGDTSDNIPGIRGIGEKTAQKLLSRYPNME
                     ELFAHCTEIPENAVRERICAGKELGLLSKELATIVKDVDIDFDFQHTHIDLPDIDEVT
                     KFLQKMQFYSFIRGIDGILGSFDKAGNGKQEIKNDVIASDSAAIQEDNSFGLLRQNSV
                     SPRNDENILPNSTSCQMQLGLFSQEVRQKINKDDIPFEVNIVSKKEDLDNLVSELKKQ
                     DLIAVDTETTSVDIQEAELVGISIAYNINEDAKMRRGVEKELRNPASTPLVFYVPLSH
                     QIGEQLEVKEVLNELKPLLEDEKIKKTMQNAKFDYNIFKNYGIKVEGIIFDTMLASYI
                     KDPSRKHGLKTQSLEHLNHVMQEITELIGSGKNQISMECVSIDEAANYACDDAYATLK
                     LTEFWKKSLDEKELKLLYDVEVPLALILADMEYEGVSVDVDYLNKLSAEFSKKVSQLE
                     EKIYELAGVPFNVNSPKQVGDILFNRLQIPMKKKRGKDNYSTGAEVLEELAGEYKICE
                     YLLQHRKYSKLKSTYTDSLPQLISRKDGKIHTSYNQTVTTTGRLSSSNPNLQNIPIRT
                     EEGNKIRNAFVASDKDGCLLSADYSQIELRLLAHVSRDDNLIEAFCSGEDIHTLTASK
                     VFEVPIEGVTKDMRYKAKAVNFGIIYGQSKYGLSKSLNIAPFAAEMFIEKYFATYPKV
                     REYMQNTIKFAHENGFVETMFGRKRYLLNELSSPNNQIREFAQRAAINQPLQGAAADL
                     IKMAMIELDKKLKQNNLKSKMIMQVHDELILDVAKDEFDMVKKLTVEAMELNQPLLVP
                     LVVDVNFGKSWIESA"
     gene            complement(27809..28000)
                     /gene="rpmB"
                     /locus_tag="PKI94_03750"
     CDS             complement(27809..28000)
                     /gene="rpmB"
                     /locus_tag="PKI94_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNW25885.1"
                     /translation="MSRQCEICGKTPLKAAKLTFSHKQNVHRQAPNLQTIKANVNGTV
                     KKIKVCTSCLKANKVQKAV"
     gene            complement(28097..>29139)
                     /locus_tag="PKI94_03755"
     CDS             complement(28097..>29139)
                     /locus_tag="PKI94_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25886.1"
                     /translation="PGLFKKDKAQNPVTPSDWTDVRLLDAGDEQNINQDVYSLYVQFV
                     DMALALGIDGIRADVPALKPAAFWKKLIAETKGRNPEILFLAEASELWNEPVCDGAVF
                     TSYKKLLDAGFDGYYGNYFNIKNWKTSQDLYSALKADEELSKKYDGKIATIGSFSTHD
                     ELSPILINGKNYSKMIIWLNATLPLNSYFIDGFDTGDDYIYFWANRKAPKTFTDDEDY
                     FVHRGKIDIFNFSRMPGGKSFDIKQEFVTANNLKTVICDAFSKGEFTIHKTSSKSVFA
                     YSRTLDQEGIIVIGNLDFRKSQNIVAHIPKIKQGSLVVPIKMGNIPKVCDGKISLQLD
                     PGEILVMYVPKF"
BASE COUNT         8785 a         5485 c         4716 g        10053 t
ORIGIN      
        1 ttgcaaggac aaggcgaaga agttgaagaa gaagatgaag atgccaccga agaattgcat
       61 gaaaaagccg atagaaagtt tgaagaagaa aaagctaaac ttaatagcca agaaactatt
      121 cttgacttag aacaaaacga tttggagacg caacatacag cattagctgc agatattgat
      181 tctgtaaaaa aattaattgc tgatgctatt aaaatattta aagtctttga aggctaatta
      241 acaggataaa ccctttgatt gtaagcatca taaatgctta tatcaatcct ttttccgatt
      301 cgagtaaaat atcgaatacc cactctataa tttcattata gagtttcttt ttgcaataaa
      361 aagggagcaa atgctccctg aaattagtcc cattgtgttt gttaattatg aacctaccag
      421 cttaagagct tcttccaaaa gctgcttttg tgattggatt agtttattag acaattccaa
      481 ttgaagcttt gcctgttgaa aataagctat gttatcctta aagccggtat ttgataactc
      541 taaaagcccc gaacgaattt cgccccttcc gataaccggt ttacccgatt cttctgttgc
      601 aacgtattga ccttgtttta tttcgtaaag cccctgagga ttatgaaaat tcattgtagc
      661 gattctatac aaaggcaccg ttcttttgcc gccttcggct ttaccgtaaa cagttccgtc
      721 gccttttatt tcataatcat cgtatttacc gagatattta ccgttattag gatcaatcca
      781 aagcttaata ttagtagtcg gaatattcat cgaaccgcct tttagcgcgg tttgctgata
      841 taagtcagca tcaactgact ttatcccttg cataatttca cctttatcat taaggatgta
      901 accctgcacg gtatatccgt ctttattttt atacacacct tctttgtcaa aagtaaaatc
      961 accgtgtctg gtataaatgc tttgaccttc ggctgttctt gtcaaaaaat aaccctgacc
     1021 ttccaaaaac aagtttgaat ttgccgtgcc gggagtagat ttaccctgcg ataaattttt
     1081 gttgtaattg tctgaaacaa ccccgcctaa aaaagtactg aaagaaactt tgttttcttt
     1141 aaagcccgga gtgtaaacgt ttgaaaggtt ttcagccaac acgtccatcc attgggtagt
     1201 ggctttttgg gtgttaagtg ctgtgataaa tgaatcattc attatctttc tccttatttt
     1261 tttgattatt gttattttgc tgctgttgtt ggttgtgcct tctgtaagat aaattttcat
     1321 aaggcaaatt ttgctgatta aaagactgtt ttaacatagg aagatttaat ttcagatatt
     1381 cttcaactgc tttatcgccc ggaataaact ctgcagaaat tttcccttgt ttgtctattt
     1441 ttaaaataac tgaaatgtct ttatcaaaat ctatcctaaa aggcttgtta gtttttaccg
     1501 ctttttgtaa agcatccata agtgttgccg aaacctgaat tgaacgttcc acttcttttt
     1561 catctgcttc tatcagaact tctttaccga ttgcctgaaa atatgcaata ttgtttttta
     1621 ataaatttac aaaaaacagt ccgtcattga cttccatttt taccgttttg tagtcaacaa
     1681 ctacttcaag cttcgcctta gaatcttttt ttacttttgc atgtgtaaaa tccttgcttt
     1741 cgtttttaac atttataaaa cgttcaactt tgtctgtttt agcaacctca ttaattgtct
     1801 ttacaaaagt ttcctttttt atatttaaag cttcatcttt ttttatcaat tttttagcag
     1861 atttaatctc cggattgaat tttacttgcg tttgaacctc aactgtaatt tcttgcttgt
     1921 tgttagcctt taaaactgta tttgattgta tattttgaga aattatttct ttgggttcgc
     1981 cttcagtttt aaaatctttc ttttgttcat ttttcatttt aagatttann nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga caagaggttt tgtttcgaca ggctttagca
     2161 tttcaggctt tacttcagct tttatctgga caggttttgc tgtttcaatt tttgtttcaa
     2221 tttttgcttc aacttttttc tcaacaggtt ttgcctcatc ggttttaaca aagtcaggca
     2281 taatatcttt aactattcct gcggtttgtt tttcttcaat tttattaata acttgttttt
     2341 cttcttgctt aacaaccgcc ttaacaaccg gcttgatttg attgtcaaca acagacttgg
     2401 tttcagtttg catttcaggt tttattttaa caggcgtaat cacttcaaaa tcaaaacttt
     2461 tcatttcact ttttattttg ttttttggtt cggtttccgg ttcaactttt tgttcaaatt
     2521 ttatttcatt ttttggctca acgccttgag gcaaaatctc ttttacggta tcgacaagag
     2581 gttttgtttc gacaggcttt agcatttcag gctttacttc agcttttgtt tggacaggtt
     2641 ttgctgtttc aatttttgaa ttttgaacaa catttagagg cgaagggcat tttaaagcct
     2701 cattttgagc tacaacaggc atagaaaagt ttttattaac agtttttata gattcgtcaa
     2761 cggcttgatt tataaactgg ttaattcttt cttccgcctt taattcaaca ttttttttat
     2821 cattttttat ttcagaaatc gcatttattt catttttgaa agacgaagtt tcaatgtgag
     2881 gcaaaaaatt tatgctttgc attgaaatat tattgggatt aatcttgttc tcttttttga
     2941 ttttcggagt atcaatattc atttaaagtt tctctttcta tctgctctat ttgtcttaaa
     3001 agttcttttt cttcttcttc gtctttttgc tttttctgct gaaagtgtct ttgaacgccg
     3061 acttcagata attgttttaa ttctgctttc ttttgctcgt ccagataagc ttctttggct
     3121 ctttctttat gcttttcaag aactttaacc gctttttcgc attcaaccag aagttctatt
     3181 tcttctctga gtttttgttc tttgtcctgt cttatttgtt caagcatttg tgccttttcc
     3241 cataagtgct tgaggtattt gtcaaaagcc tccatcaaaa gggcgtctgc ttttctctgg
     3301 ttcactttta tttcttctat ttcggcatcg tttctcctga tattttcttc tgctattgca
     3361 agtactcttt gcgccttctg tacagcaaga agctgttctt ccttttttcg gattctgaaa
     3421 tcgagtattt tttgtaatct atagacaaaa ggcatgatat aattacactt tcacacatta
     3481 attatgtccc attgcacctt ttttttacac atctaaatgt atgattgtgt ttttgtttta
     3541 aggattttaa tataaaagtc aaagaaagag cggagggaaa attaaaaatt ttccctccgc
     3601 tctttcttat aaaaatttaa tcaacaatct gaattctgcc gtcaagttga atttcaatag
     3661 gagatgcggc ctttttaata taatcgattg cgcacttatc cttatcgaaa tcaaatctgg
     3721 ctaaagccga attcctttta tccaacatcg aatatccgtc accgcctctt gttaaaaagt
     3781 cgtgcatacc tacggtatat tttttatctg tactcgggtt atttatatcc actattgttt
     3841 ctttattgta cctgtctatt atttttgctt caagtaattc gccggattta tttattttat
     3901 accttatacc tgcgacttgc atgattccgg gcttgaaatc aggcttggtt aaagactttg
     3961 ccccgaattt taatgcatct acaatttcct tttctgtaag ctcaactatt gtcattttat
     4021 ccttaaatgg ggtaagactg gttatatccc gtgtgtaaac atctccgaca acaaattcgc
     4081 ctctcatgtt tgaagcgtta atcaaggcaa tatccgtttt aagttcgctt tttatagcgt
     4141 ctgcaagaaa acttgcgtta ggattttctt ctgcaagcct gtttaattct ttatcataag
     4201 cttttgatat aaaacctact ttttcggatt ttcctataat tgtgtcaaaa aagtattgca
     4261 taggtgcatt ttttgcaaaa cttttcgttt cttgaacatt attttggact tgtgttataa
     4321 ctccgttttc attaaattcc aaattaagta ttccgaaatt attcccgtct tttcctgctt
     4381 gtgtaataac aacaggttca cctgttttat ttgaatataa aagattttta ccttcttcaa
     4441 cgccttttaa caaatcatgc gaatgcccgc cgataattac atcaattcct gaagtttcct
     4501 tagcgattcg tttttctaat tcataaccgc catgcgagag caaaattatt ttatttatgc
     4561 cttgttgctt aagattgtct atttcttgtt gaacttcttt tatggtttga tcccactcat
     4621 ctacggttat tccggcaaaa ttttcctgat tttttattct taaaaatata tcgcttggca
     4681 taatacccat aatgccatac ttgtttccgt taatttcttg aatacaagat ttcaaaattt
     4741 taccgttcca agggttatta tcaacaatat tggcatttaa tccaagcagc ttataattat
     4801 gattttttgt taactcaata agcatttcag gaggaatatc acactcgtga ttgcccacag
     4861 cggttgcagt tatacctatt actttcaaaa actctgcaat tgctttattt tctttaaccc
     4921 ctgagcccaa catggtatcc cctgaagata atttcagctt gtccgtttta gactgaggcg
     4981 taaatgaatc gaactggtct gaagcagttt ttatcctctc catttttgta acttgaccat
     5041 ggatgtcatt tatataaaaa atgctggctt tagttgtctt cggtgtttgt gatattgaat
     5101 ttaccatgtt ctccccactg ttctgttctc aattatctta aatcccgtaa aaaaaaattt
     5161 tgatactatt taaagttttt gttacatata ttttacataa aacataataa atctaaaaat
     5221 gttcatatag ttgccaaaaa gtttaccaaa gcatataatt taaatcatgc aaaacaattc
     5281 ttacaggaac gccattaaat ttctgcccac tctgtttgac aacaaaatcc aaatggacac
     5341 aagtgcagag gcactttttg ttgcaataaa aagaattatt aattttgacg atgcctacat
     5401 tttcttttta aaccccgaaa gtattcagtt aaaatacatt tacggcaagc attctatcct
     5461 taagcccgaa cagacttttt ttgtaaacga caggcttaaa agagaacttt ttagcgaaaa
     5521 atctcaaatc acagacaaaa attcgcacct gatatcagca cttaaagttc ttcattacaa
     5581 ttcatttctt gtctcaaaac tggcaataag aaataccgtt tacggatttg tactgctttg
     5641 caagaaagaa aataaatttt attcgacaca agatgtggaa attacagaaa gcgtttctgc
     5701 cgtgatttct tatatgatta aagatttgga gctttcaaat gtttttaaaa tgcagctaaa
     5761 agcactgaaa gacggtataa ttcaaacgga cagggcttat aaaacgataa aagaacaaaa
     5821 tttaaaaatt cttgaagcag acaaaattaa aaacgaattt cttgcaaaca tatctcatga
     5881 actcagaacc ccgcttaacg ctattatagg cttttctgaa gtgcttggtg caaagctttt
     5941 cggcgacttg aacgaaaaac aggaagaata tatcaaagat attcacgtct cagggcttca
     6001 ccttttagga atgattaacg aaattcttga tatctcaaag attgaagcac atgcaatgag
     6061 tcttaaccga acaaaattta tgatttcaat ggcggttaat gaagttgtaa atgtcataag
     6121 accgcttgcg gataagaaaa aaataataat tgaaaaaaca attaaaaatg atggtgaatt
     6181 ttttgcagac tttcaaaaga taagccaaat tttatacaac ctgctcagca atgccattaa
     6241 attttctaat gaagggggta aagttagaat atatgtggat ttgacatcta aagaacttat
     6301 cttaaaagtt gaagacaacg ggataggaat tgaagaaaaa ttccacggca aaatttttgc
     6361 aaaatttgtt caacttgaaa gttcttacac aaaaaaagaa agctctacag gcttggggct
     6421 taccattaca aaagaacttg tcgaaatgca cggcggtgaa atctcgctaa aaagcgaaat
     6481 aaacaaaggc agcaccttta tggctaaaat acccgttagg agtgattgtg gctaaaattt
     6541 taattgcaga agataacgat ttaaatttaa aactcatgta cgatatttta ttgacgcaag
     6601 ggcacgaagt tgattgtgca actgacggac aaatggcttt agaactgctt ttaagcagaa
     6661 aatatgattt gcttttgctc gatcttcaaa tgccaaaact ttccggcttt gacgttatga
     6721 aagaaatttt aaaacaaaaa atccaaatca aaactattgt cgtatcagcc tgcgtaatgt
     6781 ccgatgaaat caacagggca aaagatttgg gctgcgaaga atttatcgta aagcccataa
     6841 gacttcacga atttttaagc acaataaaca gagttttggg atagaggata cagaaaaatt
     6901 caaaaaatga caattttttg caatttttca aatccgacct tagaagtgct atacccctac
     6961 cggctgcggg atagccggcg gtaggggtat aagccggaca aaagaatttc atcaagcatt
     7021 tttattattt ttttgtaatc gtcttctaca accaatgccc cgcgatattt tgctgcattt
     7081 tgtattccgc tgatgtagta aggataaaac ttcctgacaa attttattcc gacatctttg
     7141 cctctaaact ctatttcacc atttaaatgt tctttaagtg cttgaatttt ttcttccaaa
     7201 gaaggttcgg acaatttttt gcctgtattt aagtaatgtt ctatccttga aatcaaagtc
     7261 acatcgccca aaacacctct tcccaaagca attccgtccg caccggatat ttcaagacac
     7321 tcaatggcac tttcaatcga atttacatcc ccgtttgcaa aaacgggaat atcgacactt
     7381 tgcttaagtt ctttgatttt tgtccaatcg gctttgcccg aatacatttg gcttcgggtt
     7441 ctggcatgaa ttgtaataaa atcagcaccc gcagcttgca tttttacccc gaagtcaaca
     7501 aaattaatct catcaagtgt atatcccagt cgaaatttta cactcaaagg cttatttatc
     7561 gcagacttaa cttctttgac aatatctgca gcaagtttgg gatttctcat caaagcacat
     7621 ccgtcttgcc ctttaaccac tttgtttacc ggacagccca tatttatatc gataatatcg
     7681 gcatattttt caagaatttt tgccgctttt gccattaatt caggcttgtg cccgctaagc
     7741 tgataggcaa taggagaatg attttcatcc ttttttaaaa tatctgcctc ttgaatttga
     7801 ataagtgctt ccgaacttat catctccgtt gtcaaaagag aattttttga atattttctg
     7861 ataagattgc gcaggacata atcggttatc cccgccatgg gtgctagcga aaactgactt
     7921 tttaaagtta aattaccgat ttttatctca ttcatactgt tttaattctt ttattctggc
     7981 ctgtgagtaa tttttgtatt cgttttgctc gggcgtcagc gaaacatatt ttttataatt
     8041 caaaagcgcg tttttatact gcattaaagc atcgtaatca agtgccatta aataatacac
     8101 aaccgttaat tccgaactgt attgaattgc ccttttataa tcggcaagtg ctatggcata
     8161 ttttttctgc tcatcgctga ttaagcctct gtaatagtat gcataagcat ttttggcatc
     8221 atctgttaaa atttgattaa taagcctaag ggcgtttaca taatcagctt tttcgtaata
     8281 acctattgct gtatccatta atttgatatt tgcctgctct acaaccgatt gatataagtc
     8341 ctgagccttg gtattgtctt tgtttatgga aagtgctttt ttaagataaa gttttgcagt
     8401 tgcccaatct tctttttccg aatacaaaac cccgatataa taagcaacat ctgaatttgc
     8461 aggtgccaaa tcaaaagcct ttttgtaata ttcgattgct ttatcattat tgtttaagga
     8521 tttatagcaa attgcaattc cgcttaaaga gtcaaaagtc gccggctgaa ttgcgctgta
     8581 tgccgcaagt gctttttgaa aatcattgtt tttataatat tcataagctt cttcgatttt
     8641 tgtggaactt tcttcttcct gaagcgaagc tatattgtcg attatttgtt tgttggttgg
     8701 aaatctgttt tttgcatttt gcaaaacctg taaagcgtta ttcaaatcat ttttttgcct
     8761 atagcaaata gcaaggttta cataaatttc aggattgttc actcttgatt ttaaaacttc
     8821 ctgatagaac aaaaccgcat cgtcgtattt tttttgtttg tgaagttcaa tcgcataaga
     8881 atacattgta tcaagagccc cgctgtcggt catcaaatcc ttttgcagat aagcaagcat
     8941 ctcggcaggc gtcattgtgg tttttaagag ttctactatt tgatttttta tatcctgatt
     9001 ggtaggatcc atgctcaaaa ctcttttgta ttggactatt gcatcttggg ttgctccttt
     9061 tgcagccaga cactgggctt tgaaaagatt ggcatcttta ttgtcaggat ataaagtcaa
     9121 aacagtatca taagctgcta ttgccgcatc ataatttttc ttttgctgat aaagcgttcc
     9181 gacattaatt cttgtggtaa catttgaagg attaagttga gctgattgtg agtaatactg
     9241 gagtgcctca tcaaatttat tttgcttttg taaaattgct cccaaatttg ccatgacttc
     9301 agcatcgtta ggtgtttgct gaagtttttg cctgtaaatc ctttcaagcg cgaataaaat
     9361 ttcaggatca tcgttacatc ttgaaagcgc aaaattatac tcctgcaccg ccacatcgtt
     9421 ttgcccaagc ctgtcaagca ctcttgcata cttaagcctt aatcccggat ttgtatcatc
     9481 tatagcaacg gccttttggt aataatcggc agcttttggg tcgttgccca gaattttcat
     9541 aatatctgca agctgctcca acgcactttt tcttaaaaca gggtcggtta aagtttgggc
     9601 aaattcatat cccgcctcgg caaaattacc gttttctctt aattcttttg ccttttgaaa
     9661 gcgcgattgg gggattgtgg aaaattttgc cgccgccaag caggtgttta aattatcagt
     9721 ggcatttgca attgcagccg ttgaattatc cgtatcttgt tgggaagcat aatattttag
     9781 gtaaaaaagt gccgcacggt aatcgtttgc cgcggcattc cagtcttttt cattgtttgc
     9841 atagtaagta ccgcgtgcaa gatatgaatt tacaagcccg actcttgccg aattgtccaa
     9901 ataatttatt ctcagcgcat ttctgaactc tgatattgcc cccgaatatt gatattgcga
     9961 caaatactgc tgacccaatt cataatgttg ggcaaaatca gccctaacac tttgaaaact
    10021 taaaaataag caaaatatta acaaaaaaag ttttttcata aacctcacct catacccatt
    10081 ttattataag caaaaaataa ttacatagac acattactat tttaacgttt aaaagcttta
    10141 gtaacagttt agaagatttt ttttgatgag aattaaacat gcatgtatat tgcaacaccc
    10201 ctcacccgac tttctaacgc tcgcgggctt ccgctcgcat tgaaagtcat cctctcccgc
    10261 aaggggcgag ggtagaaggt aaaagctaga ttatttacat attttcttat gacgaagcct
    10321 ttacaagtga gtgtttatta cgaaatgtaa caaaagtata taccttttgc aattttgtat
    10381 atacttttgt ttttatatat atgtaagttt aaataagagg caaactaaaa tggcagcagg
    10441 aatacaagca ttactaggtt ttttaatccc cgaaagaaat cgttgtacta gcgaactgat
    10501 gggaattaca gacactatat atgatgccac cgaaagaacc cttgagttaa atgaaaaata
    10561 tgaagaacaa aaagatgaaa tagaatacgc tctagacgaa aaccccaata atcaagaagc
    10621 tttagcggct cgagaagaat taagagaaca gtactattgt cagctttcag atctaaaaga
    10681 ttttcaaaaa caacttgaaa caaagaaaac aaaacttgat ggcgaatcaa catttttaag
    10741 cgcatctatt acttcatatc aaaaaatgtt gtcagatgac actaagaaaa atttcacata
    10801 cggcggcagc ggcggctaat aagatttaat aaaggataca gagaaataaa gaacactaaa
    10861 aaagaggctt ttgcctcttt tttagtgtat aattttttta tgggaagaaa gaaaaaaaaa
    10921 ttgggtgcct ttgttgtccc cacaggtgtt ggagcaagta tcggcgggtt tgccggagat
    10981 gcaagctttt gggcaagaga gttttcaaaa cactgcaatc ttatagtaaa tccgaatgtg
    11041 gtcaatgcag gctgtttttc tggaataaca gaaaatatgc tctacgttga aggctacact
    11101 ttagatgaat tttttaaagg aaaaataagt cttattcctt gtcaaaacaa caaaataggc
    11161 gtggtttttg acaaaagcat ctctcaggag gtcttgaaca tccacatcaa cacgataaac
    11221 gcagttaaaa cagtttacgg acttgacatt ttgggatatg aaattactga agaaaatgtc
    11281 ggagttgatt ttttcgttga taaatcaaat atttccacag gaagtgttaa aaatcttaaa
    11341 acccttttaa attctgctcg aaaacttata aaaaaaggtg caaatgcaat agcaatagtt
    11401 tgccgattta aagatgacaa aataaatgaa aattaccaaa acggaatcgg ggttgacccg
    11461 attggcggag ttgaagcaat aatttcgcat tatatttccc gcgaattaaa aatccctgcg
    11521 gcacattctc ccgcttttga tgatttttcg atttcaaccg aagtagttga cgcaagagct
    11581 agcgcagaat acattacacc gacttttttg ccttgtgttt tattgggact tgcaaatgcc
    11641 cctcaaattt gcaaatttag ccctaaaaaa ctcaacataa atgatttgga ttttttaatt
    11701 atgccttata actgtttagg ctcaattcct gtctttgaat caataaagcg taaaattaaa
    11761 atttatgcaa taaaagaaaa ttctacgctc ttaaatttta aaaaagagat attattcaaa
    11821 aatagtgata taattgaaat tgagaattat aaaaaatgtc tggagttaat aaagtgaaaa
    11881 taaattttaa aaaaaacgca atgacgttag ctgaattaac tgttatgttt gtcattatgg
    11941 cggtagttat aatggcaaca ttaggcattt ccaagacaaa aacagataaa ataaaagcgt
    12001 atcaatatta tgctgctttt acaaatctaa aacaggcagt aggtgaagtt tttgcagacg
    12061 gatatcggga aactcccgaa tcacctatag aaaaacaatt gaccccttat ggccacacag
    12121 gagaaaatac aggaatatgc gacaggttgg cagaagtttt taacactatc ggaaaagttg
    12181 attgtacaat ttccggcggg gataatttct cagatgaaaa tgtaaatttt accctaacaa
    12241 acggtgaaag attttttaat atcggaaaag atatagacgg tgaggcattt agattgtata
    12301 tagacattga cggcaaaaaa ggcaaaagtg tccttgacga agatgtaatg gaatttagaa
    12361 tatacagaaa cggaacggtt ttacccggag aaacttccat aggaggaaat aacaaaaatt
    12421 atctttccgc aagtataaaa gaaattaaca caaacacatg ggatcaaaaa gatattcctt
    12481 atttgaaagc tgtttgtaat gccggagaaa tgcccgaatc ttactgtaca ccattaggta
    12541 ttccgctgaa agagaattgt acttcaccaa atgtttgcca ggtatatata aataaacctt
    12601 aaattattcc aattcaagtt ttgcaatctt aaccttaaaa acgctttcta tatcaattcc
    12661 gtttataata gtggttataa ggtcattggg gttgatttta tcaatatcag aaaagtgctt
    12721 tataattcct aaaatttttg tatctgtata ttcctcaact aaccgtggaa tggtttttat
    12781 tgctgtatct tttgtgtttt cggggaaatt atttataata acccctctta cttcaattcc
    12841 gcttgaaatt gcctgattaa tcgtcaaaag cgtttggtta accgttccaa aatccggttt
    12901 tataaccacc aaaacaggaa ggttgagaaa ttttataaga ttattcatcg tgaaattttg
    12961 agcaagagga gtcataattc cgcctgcgcc ttcggtcaaa aggcaatcac atgattttgc
    13021 tatgttgtca aagtcttttt taataaccgt tttgtcaatt ttaatacctt ccagctcagc
    13081 tgcaacaagc ggttctgcct tttctttgag catataagtc gaataagttt tcacataagg
    13141 gtcaatgttt tttatatatg ccaagtcagg tgcctgcata aagccttgct tttccttcgc
    13201 tccacactga atcggtttat aaacacaggt tgtataaccc aaactctgca tggttgcggc
    13261 aagtcctgct gttataaatg tctttccgac tcctgtatct gttcctgtta taaaaagatt
    13321 taacataaga ttattttatt ataaaaattt atttattaat cgaaaaataa acttctttta
    13381 agcgtttttt gctaatgtgg gtgtaaagct gggttgtaga aacatcggag tgtcccaaaa
    13441 gttcctgaac aatccttaaa tcagcaccgt tttcaagcaa atgcgtcgca aaactgtggc
    13501 gtaaagtgtg cggagaaata tttttgtgaa ttttttctcc ctgcttgtga ataaaattgt
    13561 agatatcctg tctagtcaga aaccgcccgt tatctgaaat taaaaaattt ttcgtatcaa
    13621 gcccgttttt tttgactaaa aagtcccttt tttcaatgta ttctcttatc gcatcttttg
    13681 cttttttgcc aagcggcaca agcctttctt tagaaccttt tccgctgcat tttaagtaat
    13741 tcgcatttaa atcaaaactg tttgttttta gccccacaag ctccgaaacc ctcagcccgc
    13801 agccgtataa aagctctaca atgacttttt cggtgggatt caaaccgttt ctcaaaattt
    13861 cttctatctc ttgaaccgac ataactttgg gcaatctttt gggaactttc ggctgctcca
    13921 gcgttaaagt cgggttttga ggacaaattt catttgcgca aagccatttg aaaaaatttc
    13981 ttaaagacgc tatttttcgc attacactgg tcgaagaata atttttttca cgcaaatttc
    14041 taatgtaaga atttatatgc gttcgctgaa ttttatccaa atcaaaaaca tcaagcgcaa
    14101 aacaaaagtc caaaaaatca cttaaatcac gcctgtaagc gtcaatcgtg tttaaagaaa
    14161 gccctttttc aacctccaaa aattccaaat attctgataa aacctgcata ctcatggttt
    14221 agattataac tcatatattt taatgtgtta agtttgtaaa agttttgtgt ttttacgcaa
    14281 aaaaattcca ttttatgttt ttatatatat ataagagatt aaggaatatt tccaatgaat
    14341 gtaaacaaaa tcaacccatt ttcaacacaa gcaatgaaag gtttgcaagg gattaatccc
    14401 agccagcagc ctgttaatgc agtaaagccg gttggcaaaa atccttttga aaaacacgat
    14461 caaaaatcga aatacggcgt aggattgatt aattctgatt taaacaatat gtacacaaaa
    14521 gcccctaacg gcaaagaagg gcttgcaaat acaagatgga ttgcttaaat aaatattata
    14581 caaaagggcg gattttctct gaaaatccgc ccttttgtat gaatatctaa aaaatattaa
    14641 atccattttt gttgtctcat ttcttttctt tttttgtcgc gatgcaaaaa aagaaaagaa
    14701 accagacgga aagaaaagaa aaaaaccgcg gactaaatgc caaagcgtca gggctgtgcc
    14761 ccgaaccccc ttaaacattt acccccgacc gtatcagagt gagcaattat tacttaacga
    14821 aacaactgca aagcgagtgt gtttgagtgc cgaaggcacg agttcgcgag cttgagttga
    14881 gtttagtaat aattagcgag cgaagatccg tgtcgtggct ttttgtggta ctttttgcca
    14941 ccaaaaagta ccgaaagaga aatatacaaa aagaaaaaat gtgacattat atattaaaat
    15001 aatatttaat accaaaaggg cggattttcg tgaaacgaaa atccgccctt ttgtatattt
    15061 tataaaattt taacctgcgt aaacgcttac ctgttttctg tcgcgtctat gcggctcaaa
    15121 cttaacaact ccatcaatta acgcaaacaa agtatcatct gttccgatac caacgttaat
    15181 tccggggtga atttttgttc ctctttgacg aacgagaata ttgccggctt taaccgcttc
    15241 tccgccatat tttttaactc ccaagcgttg cgcttgtgaa tctcttccgt tacgggttga
    15301 tcccatacct ttcttatgtg ccatttttta tttctccttt taataatttt tgcttttgtg
    15361 aagtttattc ttctgaagcc ccttcagaat ctttcttttg ggctgaagtt ctgatttttg
    15421 taatcataac ttttgtaaag ccttgtctgt gcccttgctt gtttctgtaa ccttttttag
    15481 gtctttgttt gtaaacaaga actttttttt ctttaccgtg ttcaagaatt ttaccttcaa
    15541 cggaagcacc gctgatataa ggttgcccaa ctcttgattt tttaccgtta gcgagcataa
    15601 caactttgtc aaaaacgact ttgctgtcct tttcaccgtc taacagttca acttttaagt
    15661 agcggccttc ttccatttgg tattgcttac cgcctgtttc tactattgcg tacatttttt
    15721 taaatccttt cgatttaagg aatcgtatcc gcacaaataa agcttagtgt aaaagtcttg
    15781 tgcgaaattt caaacgattt gcctatctaa tacttcgtta ctctattatc acaggctaaa
    15841 aatcccctgt caaggggggt aaatgtattc ttaactcata caaaactaaa aatcacctgt
    15901 caagggggga gtagtaaata gtaaatagga aatggtgaat agagttgaaa agtcgaaagg
    15961 gtaaatgtcg ataagttgaa tagttttaaa aactcgtcgt tgcgagcgta gcgaagcaat
    16021 ccaaaaggct ttctgattta cccctcttcg attattttta tttcatcttc ggtcaagttg
    16081 tagagtttat agacttcttg gtcgattgcg tggtcgagtg tgtctatttg atttttaatg
    16141 ctattcaagg tttcagattt ttggttgtac caattcatta aatcttcttt ttgcgaaagt
    16201 gaaagtttta cgccctgttt ttctagttct tctataaacg gatgaagtcc aagagtatag
    16261 aatctttcaa ggttttggct gattttctta ggtttatatt cagtttctaa gagttccaaa
    16321 gccgatttaa cgccttcgtg tagttgttta ttgagctcaa tcatctgttc tgctttactt
    16381 gtaagattat cttgctgttc ttgcgtggct tttgctattg gaagttgccc aactccatta
    16441 gggaagtatt caaagcttcc accaccagtt tgcttcccaa tcgacctgta tctataagtt
    16501 aaaagcttag aattaagtaa agccaaaata tatttaatat tataatcttt attagtatca
    16561 aaaattacag tcatatttga aaaacttaaa tattcaaatt tttcatcaat acaaaaaaca
    16621 tttgagaaac ttctccgact gcaaaacagt ttagctttat tgtaatattc tttatgcatt
    16681 ggtctactat aacgccacca agttcttcct tcgtttttga cagttgctct attttctaaa
    16741 aattctttat tactaatcaa atgattttgt atagataatg gtaattgttc aaacttgtca
    16801 atatcttcaa aatacaggat atattcagta ttactgtcta taaaatattt atcaatattt
    16861 ttcccagaaa ctcttttttt tataaactca cttggaaatt catttggata tttgtcaaaa
    16921 ataaaaatat tatctgctgc ggtttccata ccttttccaa tatgcgtaat ttcatctagt
    16981 tttggatatt tactgtcaat cttatcatta atgcttgaaa tcttactatt aaccaatgca
    17041 aaaggcatat caagttgaaa ctcaacatca aaaaagtttg atttagtatt aatagtattt
    17101 aaaataatat cttcggtata ttgtttttct tcaaagctaa atgcccgtgt tttttcttta
    17161 tttccatttt tttgcaaaat taatatgcaa gatgttattc ccgcatcttt aaaaacataa
    17221 tattgttcaa aattcactat ttctaaaata gagcattgtt ctaaaatata atttctcaac
    17281 tttttagctt tgtccgaaaa caaaaaagca tttgagataa tgaatcccat aattccgtta
    17341 gcttttaata agtcaatagc tttttttata aagtaaaata aaatatcgct cttatccata
    17401 taaacatctg aataagaatt ctttaagtgt tcaaaaacag gtgaaccaaa accgaaggta
    17461 tctatattga aatacggcgg atttcctata atacaatcaa aaccaccacc gtctaaaatt
    17521 tctttaaact gttcgttcca cttaaacgct ttattctctg ctatcgcttc atcatcaatc
    17581 agggaattac cgcatttgat attattatca agattttcca atggtttatc cttgcgtgca
    17641 gtttttagcc acagtgcaag ttttgttatt tcggttgatt cttcgttcag atcaacgcca
    17701 aaaatattat ttaacaatat tgaacggtct gtttctgcaa tattggtatg ttttaggagg
    17761 tttgtttgta agaactcttg cccgcctcta ttgctcgaac gttcagcatc aattttttct
    17821 tcagttctta tttgatattg ttctctcaaa aagctgtgtg cctgatttaa gaatgcacct
    17881 gaaccgcaag cagggtcaag aatttttatt gttttcagtt tgtcgggatt ttcttctaaa
    17941 tacttaccga cagtttgttc aacaatgtat cttgtaatat attcaggagt ataaaaaatt
    18001 ccgtcttttt tacgctttga ttttgttttg tcttcttcaa ctccgtcaat ttctgctttg
    18061 atttgttcca agtcgctcaa tgattgttca aagatatgcc ctaaaatatt aacgttcaag
    18121 tcgctttcaa agtcataatc tgaaagcttt gtgatttttt gccatatatc atcttttatt
    18181 acaagatttt ctaactcaat gtcggaagaa aataatccgc cgttataagc atttatggga
    18241 ggacttacat catgtctgcc ttcgtcaatg tccataaaaa gatttttaaa ctcacgccaa
    18301 atcctttggt cgctcctctc acgggattta atacctaaat cataaacttg ttttactatc
    18361 cccgaaggca acaaattatt tgtgtcctcg cagaaaaata taaataccat tctgtctaaa
    18421 agtttttgag ttttttcaag gattaatttt gctccaattt cagggttgtt ttcttttaaa
    18481 tgcttgaata attccaaacg taattctttg aaatctttgt aaaaatcttt tgtaatttct
    18541 tcttcgtttt tgcttgtatc ttttatcaga ttatccaaca gtgaatttct gtccttatcc
    18601 agcaaattct gtttgcacaa caaaaaataa aagcgtttaa actccttcgg gtcatgtaaa
    18661 tctaaaatct taaattcttc ataataccct tgtcctctgc ttttgtgata taaccttatt
    18721 tctttgaaat ttgaaacaat aatccaatcg caacgatcaa atttgaataa gtaactaaaa
    18781 gcctgactta caggtgaatc gtaatctttt cttgttgatt gttttttatc aagcggagtt
    18841 tttgcatctt taagctcaat tacggctctt gtaacattta atttttcatc ttttgtaaaa
    18901 taccccaaag ccccatccgc ttttgttccg tcaaattcgg ttttcgcctc attaatcaaa
    18961 taccattcgt caggattatc gtgcatttcg gagtagccta gaattatatt aaaaaataaa
    19021 tttaaaaaac ttgcttgtac ccttgtttct ttagtttcat cataattccc gtctcttaag
    19081 gctttttgcc aattcctgat aattttttct gcacgctcaa aatcaaaccc tttcggatat
    19141 tcaaaattgt tcaaatgagt ttgcaaaaga cgtttattaa atagcactct accaacttcc
    19201 acgaataata attcacataa caataatatc aataaataaa aatttataca aatattttga
    19261 gtaatgtaaa tgaaagttct aaatcattta ccccgattta ccccttaatc aacggagaaa
    19321 atttctctta catcatccat tgtaagcgat tttgttatat ttctgtcaac ggaaattacg
    19381 ctgtcaacaa gatttctttt aacttgttta agtttttgta ttttttcttc aaccgtattt
    19441 tttgtaataa gcctgtaaac aaagactttt ttcttttgac cgatacgata ggctctgtct
    19501 gttgcctgat cttcaaccgc aggattccac catgggtcgt agtggattac ataatccgcg
    19561 ccggtcaggt tcaagcccgt accgccggct tttaagctga caaggaaaat aggaatatct
    19621 tcgctgttgt taaatctttc aacagcaccc tgtctgtctt ttgtctgacc tgtaaggtat
    19681 tcgtagcgga tgccttcttt atcaagccat tctcttatca attcgagcat atctacaaat
    19741 tggctgaaga gcagaactct gtgtttttcg gaaattatct cctcaagcat ttcttttaaa
    19801 tgctcaaatt tacctgattc attaattttt ttgatatttt ccttatcgta aagtctgggg
    19861 tgacagcaga tttggcgcag acgcagaagt gcagagaaaa tagacattct gcttttttca
    19921 agtccgtttt gttcaatgct tttaaacagt tcttcttttg tactatcaag gacttcaagg
    19981 taaaaatctc tttgttcgtc agtaagttcg cagtatgcaa tgttttcgac tttatcaggc
    20041 aaatcttttg cgacatcgcg tttcatacgg cgcaagataa acggataaat ctgcaatttt
    20101 aagcgttttt caactgtttt gtcggcacgc tccataatcg gatttacata gcggtaatta
    20161 aattctgaaa tactgaacaa aaatcccggc ataaggaagt caaaaaccga ccagagttct
    20221 tcaagcctat tttctatcgg agtaccagaa agtgccaatt tatggtcaca gacaagtctt
    20281 ttcaccgatt gcgccgtttg tgaaagcgca tttttaatat tttgggattc atctaatata
    20341 acgtatctaa aattaatttt tttcaatttt tctatatcac gacgcaccaa agcgtaactt
    20401 gtaattacaa tatcatattt gggaatgtct ttaaaaagtt cttttcgctc tattcctgca
    20461 agtttaagac aagtcaaacc gggtacaaat ttttggattt ccaactccca gttaaaaaca
    20521 accgaagtgg gacaaataac caaagtgggc atcggcccgt cgttttcttt agctctttga
    20581 agcaaagtca atgcctgaag tgtttttcca agccccatgt catctgcaag aattccgtta
    20641 agcccgtatt tatacataaa ccaaagccag ctgaaacctt ttgcctgata ttctcttagc
    20701 tctgctttta catcctttgg aatttcaata tcttcttttg tagaaaacga agatatttga
    20761 tcccagaaat ttgaaaaatc ttcgcttaaa tcaagttcaa caccaaaatt ttcaagttca
    20821 ttcaacagcc ctgccctata agttttcacc aaaaatttat cttcattgtt gcggtaaaca
    20881 tcaaatgaat taaacgagcg catcatcgca taaatatctt gtgcaggata ttctacaaag
    20941 cccaaactcc ttacgcgagc atatttttcc ccgctttgaa ttgtattgta aacatcatca
    21001 aaactgattt tgtcttctcc cacttgtccg tagagataaa tctgaaaact gtcttttgac
    21061 tcttcgcaaa aatccacttc tgcacgaagc ttaaaagaat cttcggcaag cttaaagaag
    21121 cctaaatcgt ctttttcttc aaatttccaa ccttcgcctg cctgtttaat ataataatta
    21181 taaaaatcaa cggcattttc tttttcgagc gcaaggttat ttgtctgcat aggaacaaat
    21241 ttgcacgcca aaagcatatt gtaggcatcc tgttcatgtt ttaaatttct tttaatccaa
    21301 tactggctgt cctcttttgg cttcttaacc gtaacgtaag gagttttttc gcttgttttt
    21361 gaaaaaggaa cccttatccc gtcatattca aactccaaag aagctttcaa tgactggtcg
    21421 taatctattc ccatagttac aatattaaga ggcggttttt gctcaagcat caatttatta
    21481 atttcttcag aaacttcaac atccataatt ttttgaagtc ccggcatttc ctcataaatg
    21541 aattttccgc cgtcagaatc tttgaggtct gtaaaagaag attttacaag atattgcgga
    21601 atagcaccaa cttctatatt tatagggaaa attacgtttt tataacgccc ccatttcaaa
    21661 tgccttgaaa aatatcttat ctcctcaaac ggataaacat cgtccttatc aggtcttttc
    21721 cattcaagtg caagcattat gtttcccgcc tgagaataat ttaccgaaag cgcaagccgc
    21781 cattctttgt cggtaaactt caaacgctgc ttggactttt catccaaaac ctcgtctgct
    21841 tttgcaagca gaggaaacat gtcagcaaat ttagtaagct gaatatcata ccaacctgcc
    21901 tttttatcct gacgcgaagt ctttaaaagc tgcttaaaaa tagccacttc aaccatttgt
    21961 gaagtgttaa gttcaaagtt tttatcctgt ttttgcgcct ctattaaacc ttttaaaatt
    22021 tgttcgattg aacgaacgac ttttccagtt tctcttgaca ttacaagaat ggaaaagaaa
    22081 ttgggttttc ttttggggtt aaaatgaaat atataattgc cttttggagg ctctgtaagc
    22141 gcggtgtttt catcttcaaa actcgattca atgccgtatt ttgtttcaag aaaatcctca
    22201 aaagcatgct cgtctattgc ctttagccca acagcaacgg cgtgtttaca ccattcttct
    22261 tttaagggac aattacagcg tgcttcaact ccgccttttt tgaaaatcaa gtcggttttg
    22321 taaaaatcct gaaagtttcc ttttactttc cctttgtaaa agtttttttc cggatatcct
    22381 tcaagcacct ggtctttgag atggtattcg taaccgcgtc tccaacttcc gggggttgct
    22441 ttgtccttaa tgtacctgta attaaattcg tcagaactca cctatttgag tgtctcgctt
    22501 tcctttgtat atgctacaaa aattcatgca ggaaagtaat ctctattacc cccaacaagc
    22561 ataaaatatc attaacgaat aacaaaaatt attattgcat gatttcaaaa aaaaagcaag
    22621 ggggagggta gtaaatggta aatggtaaat agtaaatagt aaataggcat aaaggcaatt
    22681 acaaagaaga taagatgagt agatgcgtgg atgcgtagaa caactgctca ccgctcactg
    22741 ttcaccacta atgttaagca aaataaatac attgtttaca ttatgcctat ttttcaaaaa
    22801 agtattataa ttacaaaaca ggaggggatt ttttatgcct gaaatggcaa gaaactatca
    22861 gtatcagcaa tacagacaat atcaaaaccc cgcatacggg caaagaccgg cttacggata
    22921 caggcaaact tatccgcagg ttgtcactcc tgttcgccct gttgcccagc ctatcagaaa
    22981 aaaggtcgtt cgcaagcgca aagtaaaccc tattgcaaga ataatttctc tttctcttct
    23041 tgctcttatc ggatattatg tccttccctt cggatttcaa aggatttcta ccccactaat
    23101 ttcactttcg ggaaattctt ataaagaaat tcaattcaat aaatatcagc ttttaaacac
    23161 taaacgagaa aaaattcctt acacttacga aattttaaaa cccacaacca attatctttc
    23221 aaatgattct tttatgaatt tgagaatgct ttctaccgca aaagccaaaa aacccgatat
    23281 gacaaccctt tacatgacag acaaaatgca ggtcgttgaa gcaaagctca aaaggcttat
    23341 gtctgaatat aaatcaatag agccttccat ttttgtctgg gaatatgatt cgggaaaatt
    23401 tgtagatatt gacgctgaaa aaacctttcc tgccgcaagt ataattaaaa tccctgtttt
    23461 aataaggctt tttaaatcca tagaggcaaa tcagcttact atttatgacg aaatggccat
    23521 gacagattat tttaaagcag aaggctcggg aaatctgcaa tcctggggca taggcaaaaa
    23581 atacactata gaccaacttg cgcgctacat gattacggaa tcggacaatt ctgcgacaaa
    23641 tatgattatg tctaaaatcg gctctatgca agacgtaaat cagggcataa gaagctgggg
    23701 aattaacagt acccgcgttc aaacatggct tcccgatatg gacggaacaa attacacaac
    23761 cgcaaaagac ttagccacaa tgctttataa tcttgacaat ccgggctttt taaatattaa
    23821 ttcgcgcgaa tacataatcg attacatgag ccacgttgaa aacaacagac ttatacaggc
    23881 aggacttgac cctaaagccc tatttgtcca caaaacaggc gatataggaa aaactttagg
    23941 cgatgcggga attgtttttg caccaaacgg caaaaaatat atatgtgtaa ttcttgcaaa
    24001 ccgcccctac aattcgcctt taggaaaaga ttttatccaa aaagcctcaa atataattta
    24061 tacttcaata acttcgggtg tttattaatt tataaaacat taagtttttc tatcggagtg
    24121 ttgagattga gctcaacgta tttgaacatg ttttgaacta taccgacagg gaaaaaataa
    24181 atgttgtcgc aaggagtaat ttttaacatt gaattttcag catttaccga aatgacatta
    24241 gccaaaaact gctttttggc aacagaaaac aaaatataac cgctttttga ctgaatttcg
    24301 tcaatagtaa atctgtttgc gttaacagcg gcaagcgtta aataaaaaag cttctgcgcg
    24361 tcaattttaa aaatctttct gcaaagacca aaatcaaccg cctgaggctg aacggcttgc
    24421 gcagcagcaa gggtttgctg cgcttgaagt acaacgggcg cacttgcgcc tagggcgcaa
    24481 gcgcgcccca aaaaaggggt aaaaagtaat aatatcaaaa ctctaactgt catcccgaac
    24541 ttgtttcggg atctatccaa tgtacaatgc cggaaagatg ctgatacacc gcaagcggta
    24601 ctctcccttg taacactcgc taaagctcgt gaacaataag agaagacgag ttcagcatga
    24661 cgatttatta tttttttaat cattttttta atcactcatt tttacctttt aatttttgga
    24721 ttgcttcgct atcgctcgca atgacgaaaa atttttcaac cattcaagca ctctcaatcc
    24781 atgatttgcc gaaattaacg tcaacaacaa gcggaaccaa aagcggctga ttaagctcca
    24841 ttgcctcgac ggtaagtttt ttcaccatat caaattcgtc ttttgcgaca tccaaaatca
    24901 attcatcatg gacctgcatt atcattttag acttcaaatt gttttgtttc aactttttat
    24961 caagttcaat catcgccatt ttaatcaaat ccgctgcagc accttgtaga ggctgattaa
    25021 ttgccgccct ttgggcaaat tctcttatct ggttattcgg acttgaaagt tcattgagca
    25081 aatatctttt cctaccgaac attgtttcta caaaaccgtt ttcgtgagcg aatttaattg
    25141 tattttgcat atattctctt actttagggt aagtcgcaaa atatttttcg ataaacattt
    25201 cggccgcaaa aggtgcgata ttcaaagatt tggaaagccc gtatttgctt tgaccgtaga
    25261 taatcccgaa atttaccgcc tttgctttat atctcatatc ctttgtaaca ccttctatcg
    25321 gaacttcaaa gacttttgac gccgtcaagg tgtgaatatc ttcgccggag caaaaagctt
    25381 ctattaaatt atcatcgcga gaaacatgcg ccagcaaacg aagctctatt tgagaataat
    25441 ccgctgacaa caaacatcca tccttatcgg atgctacaaa agcgtttctg attttatttc
    25501 cttcttccgt tcttatggga atattttgta aattagggtt agaagaagac agtcttccgg
    25561 tcgtggttac tgtttgattg tagcttgtgt gaattttgcc gtcttttcgg cttataagct
    25621 gaggcaaact gtctgtgtaa gttgatttca gttttgaata ttttctgtgt tgaagcaaat
    25681 attcacaaat tttgtactcg cctgcaagtt cttccaaaac ctctgcgcct gtcgaataat
    25741 tgtcttttcc tcttttcttt ttcatcggga tttgaagcct gttgaaaagg atatcgccga
    25801 cctgttttgg cgaatttaca ttaaaaggaa ctcccgccaa ctcataaatt ttttcttcca
    25861 actgcgaaac ctttttgcta aattcggctg aaagtttatt caggtaatcg acatcaaccg
    25921 aaaccccttc gtattccata tctgcaagga ttagcgcaag cggaacttca acatcgtaaa
    25981 gcagttttaa ctctttttca tccaaacttt ttttccaaaa ttctgtgagc tttaatgttg
    26041 cataagcatc gtcacaggca taatttgcag cctcatcgat tgaaacacat tccatgctta
    26101 tttggttttt gccagagccg ataagttcgg ttatttcttg cataacatgg tttaaatgct
    26161 ccaaagactg ggtttttagc ccatgttttc ttgaaggatc tttgatatag cttgcaagca
    26221 ttgtatcaaa aattattcct tcgactttta tgccgtaatt tttgaagata ttgtaatcaa
    26281 attttgcatt ctgcattgtt tttttgattt tttcatcttc aagcaaaggt ttcaactcat
    26341 ttaaaacttc ttttacctca agttgttcgc cgatttgatg ggacaaaggg acgtagaaga
    26401 caagaggcgt agatgcgggg ttgcgtagtt ctttttctac gcctctacgc atcttcgcat
    26461 cttcgtttat attgtaagcg atagaaatac ctaccaattc ggcttcttga atgtcaacgg
    26521 aagttgtttc ggtatcgact gctatcaaat cctgtttttt aagttcactg actaaattgt
    26581 ccaaatcttc ttttttagaa acaatattga cttcaaacgg aatatcgtct ttgtttattt
    26641 tctgcctgac ttcctgagaa aacaatccca actgcatttg acaagatgta gagtttggta
    26701 aaatattttc gtcattgcga ggagaaacag agttttgacg aagcaatcca aaagaattat
    26761 cttcttggat tgccgcgcta tcgctcgcaa tgacatcgtt ttttatttcc tgtttcccgt
    26821 ttcctgcctt gtcaaagctt cccaagattc cgtcaatgcc tctgataaaa ctgtaaaatt
    26881 gcattttttg aagaaatttt gtgacctcgt caatatcagg caaatcaata tgtgtgtgtt
    26941 gaaaatcaaa atcaatatcc acatctttta caatagttgc aagctctttg ctcaaaagtc
    27001 ctaattcttt tccagcacaa attctttctc ttacggcatt ttcagggatt tccgtacagt
    27061 gcgcaaaaag ttcttccata ttaggatatc ttgataacaa tttttgcgct gtcttttcac
    27121 cgattcccct gattccggga atgttgtcag aagtgtcgcc ccttaaacct ttataatcgg
    27181 taacctggtc tggataaacg cctaattttt cgtaaacttt attccaatcg tattctatta
    27241 attcaccttt tgaaggaatc aaaactttaa cattaccctc tttgtcaatt aactgaaaag
    27301 aatcctgatc acctgttaaa ataagtgttt tgtgccctaa tttggttgct ttatcacaaa
    27361 tagtcccaat aacgtcatcc gcttcatagc cttcttttgt gtaaatggga atgttaaaag
    27421 cctttaatcc ttcaaaaata aggctcattt ggcttcttaa agtatctggc atggcttctc
    27481 tgtttgcttt gtactggtcg tatttttcga ctctgaaagt ctttctaccc acatcaaaag
    27541 caactgtaat ggcatcgggt ttaatgtttt ggtctttcaa catatcaaaa acagctttga
    27601 aaaacccgaa cacagcccaa gtcggctgat tgtcggttgt tttcatgcct gttctttcca
    27661 acgcaaaata catcctaaat gccagtgcgt gcccgtctat taaaattaaa gtcttttgat
    27721 ttgacataca tactccctat aaaaaatttt ataatgaagg cgcggatttt gcaattgcaa
    27781 aatccgcgcc ttcaaattca aatatccctt aaacagcttt ttgaactttg tttgctttca
    27841 agcaagaagt gcaaacttta attttcttaa ccgtaccgtt aacattagcc ttaattgttt
    27901 gaaggttagg tgcttgacgg tgaacatttt gtttatgaga aaacgtaagt ttagccgctt
    27961 taagaggtgt ttttccgcat atttcgcatt gtctggacat gtttgtaatt ccttattaat
    28021 tataactagt gctttttcag cgtatctcaa aaaaaagaga tttacaagca ctttgttaaa
    28081 attgtgttaa aaaaccttaa aatttgggga catacataac taagatttct ccaggatcaa
    28141 gctgaagcga gatttttccg tcacaaactt ttgggatatt gcccattttt ataggcacaa
    28201 caagtgaccc ctgttttatt ttagggatat gggcaacaat attttgtgat tttctaaaat
    28261 caagatttcc tattacaata attccttctt ggtctaacgt tcttgaatag gcaaaaacac
    28321 ttttagaaga agttttgtga attgtaaact caccttttga aaaagcatca caaataactg
    28381 ttttaagatt attcgcggtt acaaattctt gtttaatatc aaagctctta ccccccggca
    28441 ttcttgaaaa attaaaaata tctatttttc cacgatgaac aaaatagtct tcatcgtccg
    28501 taaaagtttt tggtgctttt ctgtttgccc agaaataaat ataatcatct cccgtatcaa
    28561 acccgtctat gaaataagag tttaaaggca aagtagcatt tagccaaatt atcattttac
    28621 tgtaattttt tccgttaatt aaaatcgggc ttaattcatc atgcgttgaa aaacttccga
    28681 tagttgcaat ttttccgtca tattttttgg ataattcttc gtctgcctta agggcagaat
    28741 ataagtcctg tgaggttttc caatttttaa tgttaaaata atttccgtaa tagccgtcaa
    28801 agcctgcatc caaaagtttt ttgtaagaag taaaaaccgc tccatcacaa acaggctcgt
    28861 tccacagttc ggaggcttct gcaaggaaaa gaatttcagg atttctgcct tttgtttctg
    28921 ctattaattt tttccaaaaa gcagcaggct taagcgcggg cacatccgcc cttattccgt
    28981 ctataccaag agctaatgcc atatccacaa attgaacata taaactataa acatcttgat
    29041 ttatgttttg ttcatctcct gcgtccaaaa gtctcacatc tgtccaatcg gatggtgtaa
    29101 caggattttg cgctttgtct tttttaaata atccgggtc
//
LOCUS       DAOQAU010000015        28937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1612_length_28937_cov_5.714701, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000015 DAOQAU010000000
VERSION     DAOQAU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 28937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28937
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1612_length_28937_cov_5.714701"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1065)
                     /locus_tag="PKI94_03760"
     CDS             complement(61..1065)
                     /locus_tag="PKI94_03760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNW25887.1"
                     /translation="MNEIKKILAINFGGIGDEILFLPTLISLKKAYPDAKITLALEPR
                     SKGIKDLTDVIDDLFLIDIKFKNKYLQLLKLIFLSRKGKFDLVISSGANKFISILLFL
                     TGIKQRYGYNTGKLSQKLLTVAVPLNKNQYAASMYHDLISDITDIKTKLPEINVEKVQ
                     KEPNTVLIHPGVSKLSTKLGMIKTISPVKWAKVIDLLLEKGKKVILAGGPDDRECIAQ
                     IKSTINNFENSNFIDAYGQTKNLSDLAKLIASSEKFLCSDSAPLHIAVALKTRTYVIF
                     GPTDDKILIPQTPVINAIKADDNCELKPCLWQKRQTTCQELSCLDILPEDIVHKVLLD
                     "
     gene            1110..1949
                     /locus_tag="PKI94_03765"
     CDS             1110..1949
                     /locus_tag="PKI94_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNW25888.1"
                     /translation="MSDEIKLFEENKIRSLWDEKIEKWYFSVIDIISILTESKNPRRY
                     WSDLKIKLKEDEGFEQLYEKIVQLKLLSTDGKKYLTDVADAETMLRIVQSVPSKKAEP
                     IKQWLAKVGYERMQEMADPAKAIDRARSDWKKYGRSEKWIQQRMMGQETRNKLTDYWK
                     DHDIKEGKEYAILTNIIHQEWSDLSVKGHKDLKGLKSQNLRDHMSEAELIFTALAELS
                     TRQIAEVNDATGLDENKTAAKKGGNIAKNARKQLEEKTGKKVVSSQNYLPQRKQINKK
                     GAL"
     gene            2090..3646
                     /locus_tag="PKI94_03770"
     CDS             2090..3646
                     /locus_tag="PKI94_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNW25889.1"
                     /translation="MVSRVTTATTIGLNAHKLDVEVDFVHSLPNISIVGLPDIAVNEA
                     KERVRSAIKNSGFSFPALRVVINLAPADIRKEGTNFDLPIAIGILAENGIIEENELKN
                     SAFLGELSLDGSLRSVNGVLPLVCGLKDCGVKSVIVPSANAKEAALVEGIEVLGANSL
                     NDVVNHFSQNSEDKLTKTVVNINQYLKEHCLNNYIYDFKDVKGQQKAKKAMEIAAAGG
                     HNILMSGSPGSGKTLMAKCFASILPPLELEEALELTKIYSISGLLPSNQPLITQRPFR
                     AVHHSASAVGIIGGGSSPKPGEITLAHRGVLFLDEMVEFPRQVLEVLRQPLEDGEVVI
                     SRAQTSIKYPADFMLLGAMNPCPCGFLGDSQKNCTCSEFQIQRYRSRLSGPLLDRIDL
                     QIEVPRLSPEELLNSKQQGEPSEKIRQRVVKARQIQVQRYKGLSILTNSQLNSDLIKT
                     FCKIDETSENMLKSAIIKFNLSGRSYDRILKLARTIADLEEAKNISQSHIAQALQYRT
                     FVVKEQAGIL"
     gene            3737..5569
                     /locus_tag="PKI94_03775"
     CDS             3737..5569
                     /locus_tag="PKI94_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW25890.1"
                     /translation="MKKSLYVALIIFLVVFWLKACVNVDVQEETTLKSAQKNEQKINF
                     PPTAKTKTIDGIDYLQSQAQVGKFGGVLVSSVIGEGPKTFNPFNTKDATSAAMADLMF
                     DGLLTTQPQTGDVITKLAKSFEISPDGKVYTIKLRHGIKWSDEKPITADDVVFTWNKI
                     ILAGLGNTSTRDSVIIEGQLPKVEKIDDYTVKFTLIKPFAPFLRILAAPIAPKHVFEP
                     AVKKGAKYFDSFLSTTSKPEDFITSGAFKLKEYVPAQRVIFERNPNYYEINLKNEKLP
                     YLDKIIYLIVGDLNNEILKFEAKELDVISLRGSNVGRYKAQEVKSDFVIYNIGPDTGT
                     MFVAINMNNRKNEKGKFYIAPKKQVWFNDKNFRYAIDWALDRQAMVNNVANGLATPLF
                     TAESLNSIYLNKNLAKGHPKNIQRAKEYLKKSGFYWKNKVLYDKSGNRVEFDLYTNAG
                     NTEREAIGVMVKQDLEDLGMKVNFKPVEFNSLVNKLVNSYDWDMVIMGLTGAPLEPHN
                     GKNVWYSNGSLHLFNQRPSDDKSQRLDWETKLDKIMDEASLKIKFEDRKKLYDQYQQI
                     IYDEKPIIYLYSPVRVVAIRKKFGNIYPSTLSGVAYNLDEIYIK"
     gene            5590..6561
                     /locus_tag="PKI94_03780"
     CDS             5590..6561
                     /locus_tag="PKI94_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW25891.1"
                     /translation="MQILIYILKRILQTIPLLILVSLISFFIIRLSPVDPLAELKLNP
                     SISKETLQKETQRLGLDKPIYVQYVLWAKAFVKGDLGVCSTGERVGAKLKERIPNTLL
                     LTTLVIFLTWIVGVPLGILAAINWKTPLDRMLTVLTSIGMAIPSFFFAILLLIFAVKT
                     GWFPVGGLTSYNFSDMGLAGKIWDLTKHLILPTTVLFTISLAGLQRQMRGNLLDVLDS
                     EYVKFARAKGLSEFKVIYKHALRNAINPLITLLGFEFASLLSGAALTEFVFQYPGLGR
                     LILEAVLKSDINLVMASLMMGAIMLILGNLLADILLIITDPRIRVKT"
     gene            6668..7744
                     /locus_tag="PKI94_03785"
     CDS             6668..7744
                     /locus_tag="PKI94_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW25892.1"
                     /translation="MNNTQNTSIIKQLIKDKFALAALVVLGIIYFSLLFADFIAPYTK
                     DFSDRNMSYAPPSVIYIINEKGKLSLPYTYNWVRKYDPDSMQVEYKPDRSKKFFLKPF
                     VRGEKYKFLGLMPCSRHLIGVAPEGKLFLLGTDINGRDVFSRILFGGRISMTIGFLAL
                     LVLFPIGLLYGGVSGYFGGVVDMLMMRFAEAIMSIPSFYLLIILAAILPSNMTSIQRF
                     MLIVIILALIGWAGFARVVRGMVLSIKTQEYVQSAKAIGASNLRIITKHILPQTASYV
                     IVAMTLSVPSYILAESGLSFLGLGIQQPDASWGNMLKEAQEYINILYRPWLLTPGFLI
                     FVAVLSFNLIGDTIRDILDPKARN"
     gene            7776..8483
                     /locus_tag="PKI94_03790"
     CDS             7776..8483
                     /locus_tag="PKI94_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNW25893.1"
                     /translation="MYDLGFELLIFIRIAAAILFGFGIGLERELTNKYAGLRTHILVC
                     LGSCVFTILSIYAFPMAVDSTNPQAFGDPARIAAQILTGIGFIGGGTVLRHGSSVYGL
                     TTAATLWISASIGMACGAGFLDIAFMATVFSILVLVLIRTFEKNVLVNSAKNFKRIKV
                     IINCKEENSQQVHNYIIENFSNMHEISKKHDNSAEKSVKIEAIIDIQNNKTIQSIYKS
                     FEALNGLESVSIQETND"
     gene            8476..9783
                     /locus_tag="PKI94_03795"
     CDS             8476..9783
                     /locus_tag="PKI94_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25894.1"
                     /translation="MINKIKAIIFEYLKTFLLTIVTMFIVLVVVFVFAQHSAYNSLIN
                     NQAQDDEIEYYLIGMLIDKNKYLEEKYPKNYKINLKLGILYEVAQNYANSEQQYKMAM
                     AKAPFDEFLPTYRLANLYIKLGKINDAEELIAKIQERPNKALINYKGEIYNKLGDYFY
                     NQGDYKVAIEKYEKALFYNKILKSDNIKNINNSIASAYVYLADEEVYNMKIQDAIETL
                     KAALSIVDAPILKYKLALLFTQKDPELAYQYFEEVFKKEPSIINYDFYEQFLLHRADE
                     ALAQGQKAQSDLMQYRAKKFKEYYQNNILSVDDVSIEYCEGNIIYNKWTKKYTVNLEM
                     QLKNTSQRNLNSLFVQIIFKDENREITNYFEQVVDKNSVLKAGSLSPIINIQTSKKEF
                     NKTDSRKTITAEIYVSKLSNSYKLYLTTIPIKEQKNKSNKKTK"
     assembly_gap    9804..9903
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9904..11149)
                     /locus_tag="PKI94_03800"
     CDS             complement(<9904..11149)
                     /locus_tag="PKI94_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25895.1"
                     /translation="MTAGNLGLGCINFMGSSFMNSKIFQLTNTWNSTLGFFATQPFFS
                     AGSGYGFGFNFFEANSGLFGNNIWSSYYNALCANQTEKNPESTKSENTNPENTKLENT
                     NPTGTIPAGTNPFTQNEKNNILMKLTEIDTEIENLKLEARHALDNVKDASSENIALGK
                     AANEMQRLLDSENPSKEDLQVCLNKIKTHQGLTNQKLVVAEHELEQDIIPGLNRIDNS
                     VDVLKTQVEALDVVTQTPAANTGSTTPTTTTTTTTTTTDASTTQPPAEDPALAQAKTR
                     AQELGIKLDGTESLQQIQDKIRAKESELLAGYQAVNDDSAYRIAGKVYAIKNNKEYDA
                     LTAQDWVAIKELQAKLNIPTMTGQQFKAQHPKYKGRIYDEYSILSVGQKVNLNLLGDD
                     YREAALKLLAKAPASATSAEPAT"
     gene            complement(11290..12648)
                     /gene="rimO"
                     /locus_tag="PKI94_03805"
     CDS             complement(11290..12648)
                     /gene="rimO"
                     /locus_tag="PKI94_03805"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNW25896.1"
                     /translation="MVKKIKIALINHGCAKNLIDSELMLGLLSQKGYEITLDETQARI
                     VIVNTCSFIHDAEEESVRSILEMVNADKKVIVAGCLPQKHKEELKKAIPEIIAMVGTT
                     DFEKIVEVVDKLACCMKENSSAEVEICLTENDYISHISQKPQYIYPEAIQRQQITVGS
                     SSYIKIAEGCNYRCGYCIIPQLRGEYHSRKMENIIEEAKTLAKKGVGEIVLVAQDTTG
                     YGIDIYSKPMLPELLEELNKIKELSWIRIMYAYPSMLNDELMDKIAKLDKVVKYVDIP
                     LQHSHPDILKSMRRPAFDYEELINNIRAKIPEVTLRTAFIVGYPGEKEEHFEHLYNFV
                     EKMRFDKMGVFKYSRERNTVSYDMDGQVSEKVKQQRAEKLMELQQKISKEINQTFIGK
                     AIPCMIESIDNEGLVTARSQRDAAEIDGLVYISTNKPVIPGEIENVLIKSADEYDLYG
                     EL"
     gene            12923..13795
                     /locus_tag="PKI94_03810"
     CDS             12923..13795
                     /locus_tag="PKI94_03810"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW25897.1"
                     /translation="MKRVLITGATGLIGKEAIQPLLDAGFEVYALTIDKNSDNRVNWV
                     EANIFDFDEIKKVFEKVKPQYLLHFAWITSGDYLTSDINYKLVDASLNMLEQFHKNGG
                     KRAVFAGTCFEYEFKAEPLKESDKTNPKTVYAKCKNELRKKAQEYCIQNGISFGWGRI
                     FYVYGHGENEKRLTANVIKSIKEGRKILITAGRLIKDYMYTKDIAGAFVKFLDSGVEG
                     IVNICTGKPVSIKDFVEKITSKIGGSNLVEFIDDCANQPPVITGDNSRLINEVGYKIK
                     YDTDKALSEIIENY"
     gene            13859..14857
                     /gene="pseB"
                     /locus_tag="PKI94_03815"
     CDS             13859..14857
                     /gene="pseB"
                     /locus_tag="PKI94_03815"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNW25898.1"
                     /translation="MLNGKTVLITGGTGSFGKKFIEIAFKKYPNIKKIIVFSRDEYKQ
                     FVMQNMPEFKPYEKKMRYFIGDVRDKERLYRAFEGVDVVIHAAALKQVPACEYNPFEA
                     VKTNVLGAQNVIDAAIDKGVKRVVALSTDKACAPINLYGATKLCSDKLFIAGNAYCGA
                     KETRFSVVRYGNVAGSRGSVIPFFKKLVEEGAKELPITDMNMTRFWLKLEQAVEMVLE
                     AIENMQGGELYVKKIPSMKMPDLAKAIAPNLKVKEVGIRPGEKVHEQMITKEDARNTI
                     EFEEYYIILPEVDFEHISHKYSKGKPVAPDFEYHSGNNDRWLSVDDMKKLIEEIYV"
     gene            14850..15722
                     /locus_tag="PKI94_03820"
     CDS             14850..15722
                     /locus_tag="PKI94_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNW25899.1"
                     /translation="MCKILLLGATGLLGQALGNEARKRSHEIIGVAHSNTDFNMDISD
                     GLVLTKLIREQKPEIVINACAVVNHKLCDENIKLAYEVNARPSSIIANLANELGFYYV
                     FISTDGYYNGDKNKKHKPKDKIYILNEYARTKYAGECFALTSKNSLVVRTNIVGFRGR
                     KDNPTFLEWVINSLKEQSEMTLFDDYYTSSITVVQFSKALFDLLDKKPSGILNLASSQ
                     VSSKKEFIEKVAKEIDFSLAKTKTGSVKDLTTSIRADSLGLDVSETEKLLGYELPNLD
                     EVIEQIKKEYYELG"
     gene            15709..16788
                     /locus_tag="PKI94_03825"
     CDS             15709..16788
                     /locus_tag="PKI94_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNW25900.1"
                     /translation="MNWDKEIFINKTRIAFDAPVYFIADIAANHDGNLSRAKDLIWLA
                     KESGADCAKFQHFIAEKIVSDFGFKNLKTHQSHQSTWKKSVFKVYKEYECKRDWTSEL
                     AETAKKAEIEFMTTPYDFEILSEIDNYVNAYKIGSGDITWHEFLKKIARLNKPVLLAC
                     GASDINEVKAAVEVITKINKKIVLMQCNTNYTASLENFKYINLNVLKTFAKEFPNMVL
                     GLSNHTAGCSTVLGAVALGARVIEKHFTDNNSRTGPDHKFAMNPETWKEMVERTRELE
                     LALGDGVKRVEENEKDTVIVQRRCLRLKNDLKKGDVISESDIEILRPAPENSFPPYEV
                     EKVTGKKLNSDKKAGDAFYKGEIEG"
     gene            16803..17564
                     /locus_tag="PKI94_03830"
     CDS             16803..17564
                     /locus_tag="PKI94_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNW25901.1"
                     /translation="MSKIGLKLWNINTGNYLREAEKLYKLGLYDYIELYIVPNNTDKI
                     NLWKNLNVPFNLHAPHFEHGMNLSQKECFENNLKLYKEVKSYADVLNPEYIIFHAGTD
                     GDYRETARQITYINDQRTVIENKPYKTLPFIKGNYYVGGKIPEIKHIIDTANCKFCLD
                     IGHAISAANALGVEPYTYVEEFNKLNPIVYHISDLDTKSDMDAHLNYGTGNLDFHKIF
                     RIISSNKFITIETNKKSKDNLDNFVSDVKFLKEYL"
     gene            17561..18253
                     /gene="pseF"
                     /locus_tag="PKI94_03835"
     CDS             17561..18253
                     /gene="pseF"
                     /locus_tag="PKI94_03835"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HNW25902.1"
                     /translation="MKKLAIIPARGGSKRIPRKNIKNFLGKPIIAYSIEAALNSGAFD
                     EVMVSTDDVEIAEIAKEYGAKVPFFRSPEMSNDMAMTAPVLIEVLNEYKNLGQNFEYC
                     ACIYPCAPFINSQRLKDGMNLLVESGVDSVLPIVRFSYPPQRCLVIRNEKVQMLYPEN
                     YNVRSQDLEPYYHDAGQFYCLKSSSLIKEEKLYCENTLPIILPESEVQDIDNDEDWKI
                     AEMKYKMLKSYL"
     gene            18285..18899
                     /locus_tag="PKI94_03840"
     CDS             18285..18899
                     /locus_tag="PKI94_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25903.1"
                     /translation="MVFKTEQENFWATAFGDEYIGRNDDDELLAGNVSMFSKVFAKAK
                     NVKTVLEFGANIGLNLKAIKQLLPDVELSGIEINKKAADILKEQDVKVYQGSILDFEV
                     DYKRDFSFIKTVLIHINPDELQNVYQKLYDSSSKYILIAEYYNPKPVTISYRGHSDRL
                     FKRDFAGEMLDKFGDLKLVDYGFAYHRDKSCYQDDVTWFLLEKQ"
     gene            18918..19832
                     /locus_tag="PKI94_03845"
     CDS             18918..19832
                     /locus_tag="PKI94_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNW25904.1"
                     /translation="MYNALIIGAGQIAGGYDSPQDESVLTHAHAYSKHKGFNLLGFYD
                     VDFSKASEMAKKWGVKAFKSLDEVGSVDVISICSPDSFHLASLKETLKLNPKLIFLEK
                     PLSDNLYEAKEILEISKKIPILVNYSRRFVPEIQELAKTAKGGAFGEFLYGVGYYGKG
                     FIHNGSHMTNLLDLLVGKITKVDIVHEFEDFYPNDKTKTAILSFDCNKFTMKGIDCRN
                     YTIFELDLFFEKSRVRILDSGNKLEIYEVKESGNYAGYKNLILKEVINTELNLAMLHA
                     VENIYRHLENNEPLKSTVEKAFEAINYG"
     gene            19825..20829
                     /locus_tag="PKI94_03850"
     CDS             19825..20829
                     /locus_tag="PKI94_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HNW25905.1"
                     /translation="MAKIMFFAHDPGGANAILPLIEPLEKENEVFVFAKGPALLKLPN
                     PKILEENTLKIVNPDFLITGTSASDMTEKLLWQDSKDLNIKSMAILDHWVNYGIRFSK
                     YGLNEIEKFDKKCEFLPDYIIVMDEFAKQEMIKDGVPAEIIYPLGNPHFENLLRQSKN
                     VKNVRSKFAKKNEFLITFASEPYTEDYGQGNEKKVLEDLIHIAKDKNIKIIVKLHPKE
                     DFSKYSEFKNVMFDKETAPTEVIMASDLIVSMTSMFLLEAMILGKNILSYQPNETNPD
                     KFILTRNKMLPFVKNKEDFKEKILNILNGKRYLQYNQSIEFDAVEKIIKFISEELCRK
                     "
     gene            20817..22130
                     /locus_tag="PKI94_03855"
     CDS             20817..22130
                     /locus_tag="PKI94_03855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNW25906.1"
                     /translation="MPEIKTSKLAINGGEKTRKTKFPPYLPIGEEEFENVNEVFKSNT
                     FSRFLGCWHEDFFGGEQVQALEKEWAEFFKVKHAIAVNSATSALYCAVGAIGTEPFDE
                     IIVSPYTMSASATAPLIYNAIPVFADVEPDCFCLDVKDIEKKITDRTKAIIVVDILGQ
                     PYDAEKINVLAKKNNLKVIEDCAQAPFAKYNGKFAGTLGDIGIYSLNYHKHIHSGEGG
                     ILVTNDDELANKLRLIRNHAEAVVEAKGETDFVNMIGFNYRMTEVEAAIARAQLKKLP
                     KLVEERQKNIAYLEKELSKIPCLKMPKVRESSTNVYYQHGILFDEKIAGVHRDKFTEA
                     LKAELMPIMLRETEGIKVGCGYVKPLYLQPIFQKKIAYGSKGFPWNMAGREYDYSKGI
                     CPVVEDLHFNRLLTHEYMRPGMKKEDLDDFINAVKKVWENRSELI"
     gene            22149..22793
                     /locus_tag="PKI94_03860"
     CDS             22149..22793
                     /locus_tag="PKI94_03860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW25907.1"
                     /translation="MTLKHDEIIKKQIQIYNQAIEKYGDASGAVLLNDQQSQYFRFHE
                     LTKNFDFNSDKKTILDVGCGNAELYKYLNFQGFRGKYVGVDINEKLLKQAKSRFKNIE
                     VYKKDIMKENIDTNFDYMVISGLFNLNCGQSLDWIFEFLSKLFVHCNEILAFNLISTY
                     VNFQNKEMFYVEPEKMFSFCVQHLSRRVTLAHNNLPYNYTVLVHKNENWSSVNK"
     gene            22801..23751
                     /locus_tag="PKI94_03865"
     CDS             22801..23751
                     /locus_tag="PKI94_03865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW25908.1"
                     /translation="MKIQVNENKEIWDNFVSQSPQGCIFLYSKFLDSLNKKYEIITCI
                     DDGGEVLAGCVILFDENKRPIKNIHAFTQYQGLILKDFSVLSNHKRISKEFKIIEFFI
                     EELTKKYPSLCFCQHYNFDDMRAFQWYNYHEREKGTFKIDLRYSGVLDLTKFKSFDDY
                     LMSVRPVRRQEFNKVKDIEFRWIKDEKILDGIHKKTFERQNIERSEEESALLKSITKN
                     AIDFGYGKLCAAYFENKPISSILFIYDNTTAYYLFGANDPEYRNLFGGNFLMLNMIRD
                     AFENDIEKIDFVGVNSPNRGDYKLSYNGELKPYFILKLEE"
     gene            23753..24754
                     /locus_tag="PKI94_03870"
     CDS             23753..24754
                     /locus_tag="PKI94_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25909.1"
                     /translation="MAKDFDYKGYSEEKLLRLCDETKSDRGIAKWKYWSAEVYSFGRH
                     IRDYAFYPSAFTLYCFTDHGAGAYFDEVAKHELESDAPCQFYHSPLSVELFKKVSNKP
                     CYTLFSPFVWYRRKNKIEKISEAKGTLAFPIHSTHCIDLASDMEIYIEQLKALPEEFQ
                     PVSVCLHHCDINKGQYKLFFKHNIPVYTAGDMYDQNFAQRFYDILKNFRYSTSNAIGS
                     YTYYSVEMGIPFSIYGEKLVLMNNSDSNLEKGECKFQENPQYQKIYKMFEGLNKQITP
                     EQKEFVEKHLGIYDGISRLKMAKILYLLYLKKGNPFKDLSYALKMIRRKRRIKNETF"
     gene            24741..25511
                     /locus_tag="PKI94_03875"
     CDS             24741..25511
                     /locus_tag="PKI94_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNW25910.1"
                     /translation="MRLFKKKRKYTNEFEKIFEENKVKKRFVEQIIEIEGYKFKVPDA
                     VSFAYQIKDIFLDEVYKFKPKTEAPLIYDCGANVGTSILYFKREFPNSKIKAFEADEK
                     IHEYLIENIKDFSDVEVYHKAVWVNNDNLCFNSEGADAGSIVNNFDCKQTVKALRLKE
                     LLQKESEIDFLKMDIEGAETDVIIDCAEELKKVKNVFIEYHSIVDKPQTLDKILSIIT
                     NAGFRYHLQNISNQQHPFIERNIYCNMDLQVNIFGYRS"
     gene            25533..26543
                     /locus_tag="PKI94_03880"
     CDS             25533..26543
                     /locus_tag="PKI94_03880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HNW25911.1"
                     /translation="MTQQIPIFLASDDNYAPFVATTITSIVKNTTSFINFYVLDGGIV
                     EWNKQKIQSIQSDFKNFSVEFVDMGKCGLERFPNLNHYSVNTFSRYFIPELKPDLDKI
                     LYLDIDIIVKGDIAQLYNQEMNDYPIAAILEDFYPENYKYLKQICPNYDGGSNYFNAG
                     VLLISLDYFRKNNITQQLIDTTVKLKDLIRCADQDILNFVFANNFKILDYKFDFMPDH
                     KDYIYKLGKKEAIGAIDNHVLIHYTGRKPWNTMQAENAQDFWDVAKLTPFYKELKTAL
                     DIKSKADCYDEYLKKIVLKKLTCNYKRYKVFSKLFIGGKRKRYKQKALEVKNKIDLLT
                     GQ"
     gene            complement(26548..26781)
                     /locus_tag="PKI94_03885"
     CDS             complement(26548..26781)
                     /locus_tag="PKI94_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW25912.1"
                     /translation="MFQGATPPPRCSKTCIPSIWPETFSYTTAEAMMLKYPVACFDIG
                     APAQRVKNYDKGVVISKIDAQTALDEIVNFLRK"
     gene            27174..27977
                     /locus_tag="PKI94_03890"
     CDS             27174..27977
                     /locus_tag="PKI94_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW25913.1"
                     /translation="MKISVITVCYNAKNDIEDTIQSVFKQTALENIEYIIIDGNSTDG
                     TKEIIKKYKDKISKYISESDTGIYNAMNKGIKLATGDFIQFLNAGDFYINEKVVEDFL
                     PHLQNAKSEIIFGNLFAFNKYNGDVVRINENIIDKYLFNYQSIPHPCTFFRREIFEKV
                     GLYNENYRIVSDFEWYLKYFNEYGGSYLYVDKYAIIFDLNGICSSAKFADIHRKERKE
                     VVKKYYSGLEHFIYKVSKIIFKRQHKKKYFRKMLAIIKLNKFYKKESEN"
     gene            27981..28796
                     /locus_tag="PKI94_03895"
     CDS             27981..28796
                     /locus_tag="PKI94_03895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW25914.1"
                     /translation="MPKVSIIIPCYNSGEYLDEAVESVKNQTFKDVEIIIVNDGSTDV
                     NTIEQLKKYYNTDVKVINTDNQGVSQARNTGIKEAGGEYILPLDSDDKIHPEYVESAV
                     KILDENLQIGICYCEAEFFGDIKGKWNLPKFELKDFLARNCIFCTAMYRKSAWEKVGG
                     YKKEMDKGFEDWEFWLSLIEAGGQVHRIPKILFYYRQSEFSRSANSANKNLPELIKKI
                     VMFHPKLYAENLEYLLIPWIDIFSKSILQNSKIYRIKAKMSKKFNILVMLEKR"
BASE COUNT        10173 a         4497 c         5379 g         8788 t
ORIGIN      
        1 tggcaaaatc ggcacgagga gtgccgttta gccattcgac actagccgtc tttaaagaag
       61 tcaatctaac agaactttat gtacaatatc ttcgggcaaa atatcaaggc aagagagttc
      121 ttggcaagta gtttgtcttt tttgccataa acaaggcttt aattcacaat tatcgtctgc
      181 cttaattgca tttataacag gagtttgagg gattaatatt ttatcatcgg taggtccgaa
      241 aataacataa gttctagttt tcagtgcaac cgcaatatgc aaaggtgctg aatccgaaca
      301 taggaatttt tctgatgatg cgattaattt tgccaaatcc gacaaattct ttgtctgccc
      361 gtaagcgtca ataaaattag agttttcaaa gttattaatc gtacttttta tctgcgctat
      421 gcattccctg tcatcgggtc cgcctgcaag aataaccttt ttaccctttt caagcagcaa
      481 atcaatcact tttgcccatt ttacaggcga aatcgttttt atcatgccca atttagtgct
      541 taatttgctc actcccgggt ggataagcac cgtatttggt tctttttgaa ccttttcaac
      601 attaatttca ggcaatttcg ttttaatatc tgtaatgtca gaaatcaggt catgatacat
      661 tgaagctgca tattgatttt tattcaaagg aactgcgact gtgagaagtt tttggctcaa
      721 tttgcctgta ttgtagccgt atctttgttt tattcctgtc aaaaagagca aaatgcttat
      781 gaatttattt gcgcccgatg agataacaag gtcaaactta cctttacggg agagaaaaat
      841 cagcttcaaa agttgcaaat acttgttttt aaacttaatg tcgattaaaa acaaatcgtc
      901 aattacatcg gttaaatcct taattccttt acttcttggc tcaagcgcga gggtaatttt
      961 tgcatcaggg taagctttct tcaaagaaat caaagtgggc aaaaacaaga tttcatcgcc
     1021 tattccgcca aaatttatcg ctaaaatttt cttaatttca ttcatattta tattataatt
     1081 taaatatgga aataaaatag gagaacttta tgtctgatga aataaaactc tttgaagaaa
     1141 acaaaattcg ttcactttgg gatgagaaaa tcgaaaaatg gtatttttca gttattgata
     1201 ttatctccat tttaaccgaa agcaaaaacc caagacgtta ttggagtgat ttaaagataa
     1261 aactcaaaga agacgaaggt tttgagcagt tgtacgaaaa aatcgtacaa ctgaaattat
     1321 tgtcaaccga cggcaaaaaa tacttaaccg atgttgccga tgccgaaaca atgcttagaa
     1381 ttgttcaatc cgttccgagc aaaaaggctg agccgattaa acaatggctt gcaaaagtag
     1441 gttacgaaag aatgcaggaa atggcagatc ccgcaaaagc cattgacaga gcaagaagcg
     1501 attggaaaaa atatggcaga agcgaaaaat ggattcagca gcggatgatg gggcaggaaa
     1561 cccgcaataa acttaccgat tactggaaag accacgatat aaaagaggga aaagaatatg
     1621 ctattttaac aaatattatt catcaggaat ggtcggattt atcggttaaa ggccacaaag
     1681 atttaaaagg tttaaaatct caaaatctca gagatcacat gtcagaagcc gaattgattt
     1741 ttacagcctt agccgagctc tcaacaagac aaatagcaga agttaatgat gcaacaggtt
     1801 tagacgaaaa taaaaccgcc gcaaaaaaag gcggaaatat cgcaaaaaac gcaagaaagc
     1861 aacttgaaga aaaaacaggg aaaaaagtcg taagcagtca aaattatctg cctcaaagga
     1921 aacaaattaa caaaaaaggc gcattataat caatatctcc tttactcgct aaaggaactt
     1981 gaaacaattc aagaacattt aattatacat gcgccctttt aatatattat tactcttttt
     2041 taacaaattt cattaccttg aggtataata atttaaaaag gattagaaat tggtttcaag
     2101 agtcacaaca gcaacaacaa taggtttaaa tgcccataaa ctcgatgtgg aagttgattt
     2161 tgtgcattct ttgcccaata tttcgattgt gggactgccg gatattgcag taaatgaagc
     2221 aaaagagcgg gtgcgctcgg cgataaaaaa ctcggggttc agctttcccg cacttcgtgt
     2281 tgttataaat ttagcccctg ctgacataag aaaggaagga acaaactttg accttcccat
     2341 tgcaatagga attttagccg aaaacgggat aatagaagaa aatgaactca aaaacagtgc
     2401 ttttttaggt gaactgtcac ttgacggaag ccttagaagt gtaaacggag tgctgccgct
     2461 tgtgtgcgga cttaaagact gcggagttaa aagcgtcata gtaccttcgg ctaatgcaaa
     2521 agaagccgca cttgtggaag gaatagaggt tttgggcgca aattccctaa atgatgttgt
     2581 aaaccatttt tcgcaaaatt cagaagataa actcactaaa acagttgtaa atattaacca
     2641 atacctaaaa gaacattgcc tgaacaatta tatttatgac tttaaggacg taaaagggca
     2701 gcaaaaagcc aaaaaggcaa tggaaattgc cgcagcaggc ggacacaata ttttaatgag
     2761 cggctcgccg ggttcgggta aaactcttat ggcaaaatgt tttgcatcga ttttaccgcc
     2821 gcttgaactt gaagaagcac ttgaattgac aaaaatttac agtataagcg ggcttttgcc
     2881 ttccaatcag cctttaatta cccaaagacc cttccgagca gttcatcatt cggcatcggc
     2941 tgtgggaata atcggcggag gaagcagtcc aaaacctggt gaaataacgc ttgcacacag
     3001 aggcgtttta tttttggacg aaatggtaga atttccaaga caagtcttag aagttttaag
     3061 acaaccgctt gaagacggcg aagtggtgat ttcaagggct caaacttcaa ttaagtatcc
     3121 tgcagatttt atgttgttag gagcaatgaa tccttgccct tgcggctttt taggcgacag
     3181 ccaaaaaaac tgtacatgtt ccgagtttca aatccaaaga taccgctcaa gactttcagg
     3241 accgctttta gacagaattg accttcaaat cgaagtcccc aggctttctc ccgaagaact
     3301 tcttaactca aaacaacaag gcgagccctc tgaaaaaatc aggcaaaggg ttgtaaaagc
     3361 aagacaaatt caggttcaaa ggtataaagg gcttagcata cttacaaact cgcagcttaa
     3421 ttcggattta atcaaaactt tttgtaaaat cgacgaaact tcagaaaaca tgcttaaatc
     3481 ggctattatt aaatttaatc tgtccggaag aagctacgac agaattttaa aactcgcccg
     3541 cacgatagca gaccttgaag aggcaaaaaa tatttcacaa agccacatcg cacaagccct
     3601 tcaatacagg acttttgtgg ttaaagaaca agccggtatc ttataagtgc aagggatttt
     3661 tcctcttgat attttatcat tccggcactc tccaagtgcc cctttaaatt cttctttttt
     3721 atgctaattt aattacatga agaaaagttt gtatgttgct ttaataatat ttttggttgt
     3781 tttctggctg aaagcctgtg taaatgtgga tgttcaggaa gaaacgactt tgaaatcggc
     3841 acaaaaaaac gaacaaaaaa ttaactttcc gccaacggct aaaacaaaaa ccattgacgg
     3901 gattgattat cttcaaagtc aggcgcaagt aggaaaattc ggcggggttt tggtttcttc
     3961 ggtaatagga gaaggaccta agacatttaa cccctttaat acaaaagacg ccacttctgc
     4021 tgctatggca gatttaatgt ttgacgggct tttgaccact caaccgcaaa caggcgatgt
     4081 tataactaaa cttgcaaagt cttttgaaat ctcgcccgac ggaaaggttt atacgataaa
     4141 attaaggcat gggataaaat ggtctgacga aaaacctata accgcagacg atgtggtttt
     4201 cacatggaat aaaattattc tggcggggct tggaaacact tcaaccaggg attctgttat
     4261 tattgaaggg cagcttccca aagtcgaaaa aattgacgat tatacggtaa aatttacctt
     4321 aataaaacct tttgcgccgt ttttgcgtat tcttgccgct cctattgcgc caaaacatgt
     4381 ttttgagcct gcagtcaaaa agggggctaa atattttgac tcgtttttaa gcacaacctc
     4441 aaaacccgaa gattttatta caagcggcgc gtttaaatta aaagaatatg tgccagctca
     4501 aagggttatt tttgaaagaa atcccaatta ctacgaaatt aatttaaaaa atgaaaaact
     4561 tccctacctt gataaaataa tttatcttat tgtaggagat ttgaacaacg aaattttaaa
     4621 atttgaagca aaagaacttg atgttatttc tttgcggggt tcaaatgtgg gcagatacaa
     4681 agctcaagaa gtaaaatcgg attttgtgat ttataacatc ggccctgaca caggaacaat
     4741 gtttgttgcg ataaatatga acaacagaaa aaacgaaaag ggtaaattct atattgcgcc
     4801 taaaaaacag gtttggttta acgataaaaa cttccgctac gcaatcgatt gggcacttga
     4861 ccgtcaggca atggtgaaca atgttgcaaa cggacttgca acacctttgt ttacagcaga
     4921 aagccttaat tcaatttact taaataaaaa tcttgccaaa gggcatccca aaaacattca
     4981 aagggcaaaa gaatatttga aaaaatccgg gttttattgg aaaaacaaag ttttatacga
     5041 caaatcaggc aacagggtgg aatttgactt atacacaaac gccggaaata cagagcgcga
     5101 agcaatcggc gttatggtaa agcaggattt ggaagattta ggcatgaaag taaacttcaa
     5161 gcccgtcgaa tttaactcgc ttgtaaacaa attggtaaat tcctatgact gggacatggt
     5221 cataatgggg cttaccggag ctccgctaga gcctcacaac ggcaaaaacg tctggtattc
     5281 aaacggaagc ttgcatttgt ttaaccaaag gccgtcagac gacaaatcac aaagacttga
     5341 ttgggaaacc aaactcgaca aaattatgga tgaagcttct cttaaaataa aatttgagga
     5401 cagaaaaaaa ctctatgacc aatatcagca gataatttat gatgaaaaac ccataattta
     5461 tctttattcg cctgtaaggg tagtggcaat ccgcaaaaaa ttcggcaaca tttacccttc
     5521 aacgctatcg ggagtggctt ataatttgga tgaaatttat ataaagtgag ctgctcacaa
     5581 ggacacaaaa tgcaaatact aatctacatc ttaaaacgaa tacttcaaac gataccgctt
     5641 ttaatactgg tgtcgctgat aagctttttt attatccgct taagccctgt tgacccgctt
     5701 gcagagctga aattaaaccc ttccatttcc aaagaaacac tccaaaaaga aacccaaagg
     5761 cttgggcttg ataaacctat ttacgttcaa tacgtacttt gggcaaaagc ttttgttaaa
     5821 ggtgatttgg gggtttgctc aacaggcgaa agagtaggcg caaaattaaa agaaagaatc
     5881 cccaataccc tgcttttgac cactcttgtt atttttctta catggattgt cggtgtgccg
     5941 cttgggattc ttgctgcaat aaactggaaa acgcctttag acaggatgct tacggtttta
     6001 accagtattg gaatggcaat tccgtcgttt ttctttgcga ttttgctttt gatttttgcc
     6061 gtaaaaacag gctggtttcc ggtcggcggt ttaacaagct acaatttcag cgatatgggt
     6121 ttggcgggaa aaatttggga tttgacaaaa catttaattt tgcccacaac agttttgttc
     6181 acaatttcgc ttgcagggct tcaaagacag atgagaggca atcttcttga tgttttagac
     6241 agcgaatatg ttaaatttgc cagagcaaaa gggctcagcg aatttaaagt tatctataaa
     6301 cacgcgctaa gaaatgcgat taatccgctt ataactcttt taggctttga atttgcatca
     6361 ctattaagcg gtgccgcact gacggaattt gtgttccagt atccggggct tggtcgattg
     6421 atactggaag cggtcttgaa atcagatatt aaccttgtaa tggcatcttt gatgatgggg
     6481 gcgattatgc tgattttagg aaatttattg gcggatattc tgctgataat aactgatcct
     6541 cgaattagag taaaaacata aaaaaaaata cgtcatcctg aatttatttc aggatctatc
     6601 caataaacaa agccggtaag atcccgaaac gagttcggga tgacaaaaaa aatacataaa
     6661 ggaaacaatg aataatactc aaaatacttc cataataaaa cagctaatca aagacaaatt
     6721 tgcccttgcg gctcttgttg tgcttggaat tatctatttt tcgcttcttt ttgcggattt
     6781 tatagcgcct tatacaaaag atttttcaga taggaatatg tcatatgccc cgccttctgt
     6841 tatttacata attaacgaaa aaggtaaact gtcgctgcct tacacttaca actgggtcag
     6901 aaaatacgac cctgattcca tgcaggttga atacaaacca gacagaagca aaaaattctt
     6961 cttaaaacct tttgtaagag gagaaaaata caaattttta ggcttaatgc cctgctcaag
     7021 acacttaatc ggcgtcgcac ctgaaggaaa actgtttttg ttgggaacgg atataaacgg
     7081 ccgcgatgtt ttttcaagaa tactttttgg cggaagaatt tcgatgacaa taggttttct
     7141 tgcccttctg gtgttatttc ccataggact tttatatgga ggagtttcag ggtatttcgg
     7201 cggagttgta gatatgctta tgatgcgttt tgcagaagca ataatgtcaa ttccgagttt
     7261 ttatctttta ataattcttg ccgccatttt gccttcaaat atgacaagta ttcaacggtt
     7321 tatgttgatt gtgataattc ttgccctgat aggctgggcg ggttttgcaa gggttgtaag
     7381 ggggatggtt ttgtccataa aaacgcaaga atacgttcag agcgcaaaag caatcggggc
     7441 ttcaaattta agaattataa caaaacacat tctgcctcag acggcaagtt atgtaattgt
     7501 ggcaatgact ttaagcgttc cttcctacat tcttgcagaa tcgggcttga gctttttggg
     7561 gttaggaatt caacagcctg acgcaagctg gggtaatatg cttaaagaag cgcaggaata
     7621 cataaatata ctctaccgcc catggctttt gacccccgga tttttaattt ttgttgcggt
     7681 gttatcattt aatttaatag gtgatacaat aagagatatc ttagatccga aagcaaggaa
     7741 ttaagttttt gtgttaatat aaggcaggtg tggttatgta cgatttaggt tttgaattat
     7801 taatatttat aagaatagct gcggcaattc tttttggttt tggaataggg ctggaaagag
     7861 agcttaccaa taaatatgcg gggcttagaa cccatatttt agtttgcctt ggttcttgcg
     7921 tatttaccat tctttctata tacgcttttc ctatggcggt tgacagcact aatccgcaag
     7981 cattcggcga ccctgcaaga atagcggcac aaatcttgac aggtataggt tttataggcg
     8041 gcggaacagt gttaagacat gggtcttccg tttacggact tactactgcg gccacattat
     8101 ggatttctgc ttcaataggt atggcatgcg gtgcaggatt ccttgatatc gcttttatgg
     8161 caactgtttt cagtattctt gtgcttgtcc ttataagaac ctttgaaaaa aatgtgttgg
     8221 tcaacagtgc caaaaatttt aaaagaatta aagttataat taactgcaaa gaagaaaatt
     8281 cccaacaagt ccataattat ataattgaga atttttctaa tatgcatgaa atttccaaaa
     8341 aacacgataa ttcagctgaa aaatcagtaa aaatcgaagc aataatagac attcagaaca
     8401 acaaaaccat acaatccata tataaaagtt ttgaagcact aaacgggctg gaatccgttt
     8461 caatacagga aacaaatgat taacaaaata aaagcaatta tattcgaata cttaaaaact
     8521 ttcctgctaa ccatagtcac aatgtttata gttttggttg tagtgtttgt ttttgcgcag
     8581 catagtgctt ataacagcct tatcaacaac caagctcaag acgatgaaat cgaatattat
     8641 cttataggta tgctcatcga caaaaataaa tatttggaag aaaaatatcc taaaaattat
     8701 aaaataaatt taaaacttgg gattttgtac gaagtcgccc aaaattatgc gaattcagaa
     8761 caacagtaca aaatggcaat ggcaaaagca ccgtttgatg aatttcttcc tacatacagg
     8821 cttgcaaatt tatacataaa gctcggaaaa ataaacgatg ccgaagaact tattgcaaaa
     8881 attcaagaaa gacctaacaa ggctcttatc aattataaag gtgaaatata caataaattg
     8941 ggggattatt tttataatca aggtgactac aaagttgcaa tagaaaaata cgaaaaagcc
     9001 cttttttata acaaaatttt aaaatcagac aacattaaaa atatcaacaa cagtatagct
     9061 tcagcctatg tatatcttgc agacgaagaa gtttataata tgaaaatcca agatgcaata
     9121 gaaacactga aagcagcctt aagcattgtt gatgccccga ttttaaaata taaactggca
     9181 ctgcttttta cgcaaaaaga tcccgaactt gcttatcaat actttgaaga agttttcaaa
     9241 aaagaaccct ctataattaa ctacgatttt tatgaacaat ttttgcttca cagagcagat
     9301 gaagctttgg cacaaggtca aaaagcccaa tctgatttga tgcaatacag agcaaaaaaa
     9361 ttcaaagaat attaccaaaa caacatttta tctgttgatg acgtttcaat agaatattgc
     9421 gaaggtaata taatttataa taagtggacc aaaaaatata ccgttaatct tgaaatgcaa
     9481 ttaaaaaaca cctctcaaag gaacttgaat tcactgtttg tccaaataat ttttaaagac
     9541 gaaaacagag aaattacaaa ttattttgaa caggttgtcg ataaaaattc ggtgcttaaa
     9601 gcaggctcgc tcagccctat tattaacatt caaacctcaa aaaaagaatt caataaaaca
     9661 gattcaagaa aaaccataac cgcagaaatc tatgtgtcaa aactttcaaa ttcttataaa
     9721 ctttatctga caaccattcc gattaaagaa cagaaaaaca aatccaacaa aaagaccaaa
     9781 tgataaatca tttggtcttt ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnttgtcgc tggctctgca gatgttgccg aagcaggtgc tttagcgaga agtttaagag
     9961 ctgcttcgcg ataatcatcc cccagtaaat tcaaatttac tttttgacct acagacaaaa
    10021 tactgtattc atcatatatt ctgcctttgt atttaggatg ttgtgcttta aattgttggc
    10081 ctgtcattgt aggtatattt aattttgcct gaagttcttt aattgctacc caatcctgag
    10141 cagttaatgc atcatattct ttattgtttt taattgcata aacctttccg gctattctat
    10201 aagcactgtc atcattaacc gcttgatatc cggctaacaa ctctgattct ttagctctta
    10261 tcttatcttg aatttgctgc aatgactctg ttccgtcaag cttaatgcca agttcttgag
    10321 ctcttgtttt tgcttgtgct aaagcagggt cttccgcggg gggctgtgtt gttgaagcat
    10381 cagtagttgt agttgttgtt gttgttgttg tagttggtgt tgtgcttcct gtatttgctg
    10441 caggagtttg tgttactaca tctaatgctt cgacttgagt ttttaaaaca tcgaccgaat
    10501 tatcaattct atttaatcca ggtattatat cttgttcaag ttcatgttct gctactacta
    10561 atttttgatt agttaaacct tgatgcgttt ttattttatt aagacacact tgaagatctt
    10621 ctttacttgg attttcagaa tccaaaagtc gctgcatttc atttgcagct ttgcccaaag
    10681 ctatattttc acttgaagcg tccttaacat tgtcaagagc atgtctagcc tctagtttaa
    10741 gattctcaat ttcagtatca atttcagtaa gtttcattaa aatattattt ttttcatttt
    10801 gcgtaaaagg attagtgcct gcaggaattg tacccgtagg atttgtattt tcaagttttg
    10861 tgttttcagg gtttgtgttt tcagattttg tgctttcagg gtttttttct gtttgattag
    10921 cacacaaagc attatagtaa gaagaccaaa tgttgtttcc gaaaagacct gagttagctt
    10981 caaaaaaatt gaatccgaag ccatagccgc ttccggcact aaaaaacggc tgcgttgcga
    11041 aaaagcctaa tgtcgaattc caagtattag ttaattgaaa tattttagaa ttcataaaag
    11101 aactgcccat aaagtttata caaccaagtc ctaaatttcc tgctgtcata attaatatcc
    11161 tcttaattaa aatcctctta tttatataaa aacaatccca agcccatgat tgacacatgt
    11221 ttgaaaccgt ttttagcaac aaagctcaaa acgcaatgaa tacaaagaaa aaaccttatt
    11281 tacaaaaatt tacaattcac cataaaggtc atattcatca gcagatttta tcaaaacatt
    11341 ttctatctcg cccgggatta cgggtttatt tgttgaaata taaacaagtc cgtcaatttc
    11401 tgcagcatct ctttgagaac gtgcggttac aagtccttca ttatctatgc tttctatcat
    11461 acaaggaatg gcctttccta taaaagtttg atttatttct tttgaaatct tttgctgaag
    11521 ctccattaat ttttcggcgc gctgctgttt aactttttcg ctcacctgcc cgtccatatc
    11581 gtaagacacg gtgtttcttt ctcttgaata tttaaagact cccattttat caaagcgcat
    11641 tttttccaca aaattgtaaa ggtgttcaaa atgttcttct ttttctcccg gataccctac
    11701 aataaaagcc gttcttaagg ttacttcagg tatttttgct ctaatattat ttataagttc
    11761 ttcatagtca aaagcaggtc tgcgcattga tttaagaatg tccggatgtg aatgttgaag
    11821 aggaatatca acatatttta ccactttgtc aagttttgct atcttatcca ttaattcatc
    11881 attgagcatg gaaggatacg catacattat tcttatccag gaaagttctt ttattttgtt
    11941 caattcttcc aaaagttccg gcagcatagg ctttgaataa atatcaattc cgtaacctgt
    12001 agtgtcttgt gctacaagca ctatttcacc gactcctttt tttgcaaggg ttttggcttc
    12061 ttcaatgatg ttttccattt ttctggaatg gtattcgccc ctaagctgag ggataatgca
    12121 gtaaccgcag cgataattgc atccttcggc aatttttata tatgagctgc ttccgacggt
    12181 aatctgctgc ctttgaattg cttcaggata aatatattgt ggtttttgcg aaatatgcga
    12241 aatatagtca ttttctgtca agcaaatctc gacttcggca gaagaatttt ctttcataca
    12301 gcatgccagt ttatcaacca cctcgacaat tttttcaaaa tctgttgttc caaccattgc
    12361 aattatttcg gggatagctt ttttaagttc ctctttgtgc ttttgaggaa gacagcctgc
    12421 aacaataact tttttgtccg cattaaccat ttctaaaata gagcgtacag actcttcttc
    12481 ggcatcatgg ataaaagaac aagtgttcac tattacaatc cttgcctggg tttcatcaag
    12541 agtaatctca tagccctttt ggcttaaaag cccgagcata agctctgaat caatcaagtt
    12601 ctttgcacat ccgtgattta ttaaagcaat ttttattttt tttaccatag attaatatat
    12661 tatcatgaca aaaaaaagaa tttaatacct atttgcataa agttttaact tgacatagca
    12721 tattgtttat gttataaatt ttgtggggta aatgtatatt tataagcctt ggacattttt
    12781 caaggctttt tgtttgtgaa aaattatttt ttacaaacag agcaaagaat aattcccaaa
    12841 tatacaattt gaatttttca cacccacatc tccaataatt ttttaagctt ctttatggta
    12901 aaattatttc aagaggtatg ttttgaaaag agttttaatc acaggcgcaa caggtttaat
    12961 cggaaaagag gcaattcagc cccttttgga tgcaggattt gaagtatatg cacttaccat
    13021 agataaaaat tccgacaaca gagttaattg ggttgaagca aatattttcg attttgatga
    13081 aataaaaaag gtttttgaaa aagtaaagcc ccaatattta ttgcattttg cctggattac
    13141 aagcggagat taccttactt cggacataaa ttacaaactt gttgatgcaa gtctgaatat
    13201 gcttgaacaa ttccataaaa acggaggtaa acgtgcggtt tttgcaggaa cttgctttga
    13261 atacgaattt aaagcagaac ctcttaaaga aagcgataaa accaatccga aaacggttta
    13321 tgcaaaatgc aaaaacgaat taagaaaaaa agctcaggaa tattgcattc aaaatggtat
    13381 aagcttcggc tggggaagga ttttttatgt ttacgggcat ggagaaaacg aaaaaagact
    13441 tactgcaaat gtaataaaat cgattaaaga aggcagaaag attttaataa cagcaggcag
    13501 acttatcaaa gactatatgt acacaaaaga tattgcagga gcgttcgtta aatttttgga
    13561 ttcgggggtt gagggcattg taaatatttg taccggaaaa cctgtttcta ttaaagattt
    13621 tgtcgaaaaa attacttcaa agattggtgg tagtaattta gttgaattta ttgacgattg
    13681 tgccaatcag cctcccgtta ttacaggtga taattcaaga ttaattaacg aagtcggcta
    13741 taaaataaaa tatgatacag ataaagcgtt aagcgaaatt attgaaaatt attaataaat
    13801 gttgacattt atatatgcta agttcaaaat aataataaat taaaaaattg gagaaaatat
    13861 gttaaacggt aaaacagtat taatcacagg tggaacagga agtttcggga aaaagtttat
    13921 tgaaatagcg ttcaaaaaat atccgaacat caaaaaaatc attgtttttt caagggatga
    13981 atacaagcag tttgtaatgc aaaatatgcc tgaattcaag ccttatgaaa agaagatgcg
    14041 gtattttatc ggagatgtaa gggataaaga aagactatac agagcttttg aaggggttga
    14101 tgtggtaatt catgcagcag ccttgaaaca agtacctgca tgcgaatata acccttttga
    14161 agcggtgaaa acaaacgtgc ttggtgctca aaatgtcatc gatgcagcga ttgataaagg
    14221 cgtaaaaaga gttgtggcac tttcgaccga taaagcctgc gctccgatta atctttacgg
    14281 cgcaacaaaa ttgtgttccg ataagttgtt tattgcagga aatgcttatt gtggggctaa
    14341 agagaccaga ttttccgttg taagatacgg caacgttgca ggctctcgcg gctctgtaat
    14401 acctttcttt aaaaaattgg ttgaagaagg tgcaaaagaa ctcccgataa ccgatatgaa
    14461 tatgacaaga ttttggctaa aacttgaaca ggctgttgag atggttttgg aagccataga
    14521 aaatatgcag ggcggcgaat tatacgtcaa gaaaatacct tcaatgaaaa tgcctgattt
    14581 ggcaaaagcc atcgcgccga atttaaaagt taaagaagtg ggcattcgcc ccggtgaaaa
    14641 agttcacgaa cagatgatta caaaagaaga tgcaagaaat acaattgaat tcgaggaata
    14701 ttatataatt ctgccggaag ttgattttga gcatatttct cataaatatt ctaaaggaaa
    14761 acctgttgct cctgattttg aatatcattc gggaaacaat gacagatggc tttcggttga
    14821 tgatatgaaa aaattaattg aggaaatata tgtgtaaaat tcttttgctt ggcgctacag
    14881 gattgttggg gcaagcactg ggtaatgagg caagaaaacg cagccatgaa ataatcggag
    14941 ttgcacattc aaataccgat ttcaatatgg atatttctga tggtttggtg cttacaaaat
    15001 taattcgaga gcaaaaacct gaaatcgtta taaatgcctg tgcagttgtt aatcacaaac
    15061 tttgtgatga aaacattaag cttgcttatg aagttaatgc aagacccagt tctattatag
    15121 caaatttggc gaatgagtta gggttttatt atgtttttat ctcaacagac ggttattata
    15181 atggcgataa aaacaaaaaa cataagccta aagataagat ttacattttg aatgaatatg
    15241 caaggacaaa atatgccgga gaatgttttg cacttacaag taaaaattct cttgttgtgc
    15301 gaacaaatat tgtaggcttt aggggaagaa aagacaatcc gacttttctt gaatgggtaa
    15361 taaattcact taaggaacag tcggaaatga cgctttttga tgattattac acttcaagta
    15421 ttactgtagt ccaattttca aaggcattat ttgatttgct agacaaaaaa ccttcaggga
    15481 ttttgaatct ggcaagctct caagtttctt caaaaaaaga atttattgaa aaagtcgcca
    15541 aagaaattga tttttcgctt gcaaaaacaa aaacaggttc ggtaaaagac ttaacaacat
    15601 caataagagc ggacagttta ggtcttgatg tttccgaaac agagaaactt ttaggatatg
    15661 aacttccgaa cttagatgaa gttatcgaac aaattaaaaa ggaatattat gaattgggat
    15721 aaagaaattt ttattaataa gacaaggata gcatttgatg cacctgttta ttttatcgca
    15781 gatattgctg caaaccatga cggcaattta tcaagggcaa aagacttaat atggcttgcg
    15841 aaagaatccg gtgctgattg tgcaaaattt cagcacttta ttgcagaaaa aatagttagc
    15901 gatttcggat ttaaaaattt gaaaactcat caaagccatc aatcaacttg gaaaaaatcc
    15961 gtttttaaag tttacaaaga atacgaatgc aaaagagatt ggacttcaga acttgctgaa
    16021 acagccaaaa aagcagaaat cgaatttatg acaacgcctt acgattttga aattttaagc
    16081 gaaatagata actacgtaaa tgcttataaa ataggctcag gcgacataac ttggcatgaa
    16141 tttcttaaaa aaattgcccg attaaataaa cctgttttgt tggcatgcgg ggcttctgac
    16201 ataaacgaag ttaaagcggc ggttgaggtt attacaaaaa taaacaaaaa aattgtgctt
    16261 atgcaatgca acacgaatta caccgcatcg ttggaaaatt ttaaatatat aaatttgaat
    16321 gtcttaaaga cttttgcaaa agaatttcct aatatggttt tagggctatc caatcataca
    16381 gcaggctgtt caactgtcct cggggctgtt gctttaggtg caagagtaat tgaaaaacat
    16441 tttaccgata ataattccag aacaggtcct gaccataaat ttgctatgaa ccctgaaacg
    16501 tggaaagaaa tggttgaaag aacccgcgaa cttgagcttg cgctcggcga cggggtaaaa
    16561 agggttgaag agaacgaaaa ggatacggta attgttcaaa gaagatgtct gagacttaaa
    16621 aatgacttaa aaaaaggtga tgttatttca gaaagtgata ttgaaatttt aagaccggct
    16681 cccgaaaact cttttccgcc ttatgaggtt gaaaaggtca cgggtaaaaa acttaactca
    16741 gacaagaaag caggagatgc attttacaaa ggggaaattg aggggtaaat gggggtaaat
    16801 cggtgtcaaa aatagggtta aaactttgga atataaatac cggcaattac ttaagagagg
    16861 ccgaaaagct ttataaattg ggactttatg attacattga actttatatt gttcctaata
    16921 atactgataa aataaattta tggaaaaacc tgaatgttcc tttcaatctt catgctccgc
    16981 attttgagca cggaatgaat ttgtcacaaa aagaatgttt tgaaaacaac ttaaaacttt
    17041 ataaggaagt aaaatcatat gcagatgtct taaatcctga atatattatt tttcatgcgg
    17101 gaacagacgg tgattacaga gaaactgcaa gacagattac atatattaat gatcaaagaa
    17161 cagttattga aaacaaacct tataaaactc ttccttttat aaaaggcaac tattatgtgg
    17221 gagggaaaat ccctgaaatt aagcacataa ttgataccgc aaactgtaaa ttttgccttg
    17281 acatcggtca tgcaatttca gcggcaaatg ctttaggcgt tgagccttac acttatgtag
    17341 aagaatttaa taaacttaat ccgattgttt atcatatttc agatttagat acaaaaagcg
    17401 atatggatgc tcatttgaat tacgggacag gaaaccttga ttttcacaaa atttttagaa
    17461 tcatttcaag caacaaattt ataactatag aaacaaacaa gaaaagtaaa gataatttag
    17521 acaattttgt ttcggacgta aaatttttga aggagtattt atgaaaaaat tggcaataat
    17581 tcccgcaagg ggcggcagta aaaggattcc aagaaaaaat attaaaaatt ttttaggaaa
    17641 acctataatt gcatattcaa tagaagcggc gttaaattcg ggagcttttg atgaagttat
    17701 ggtttcaaca gatgatgttg aaatagcaga aattgcaaaa gaatacggtg caaaggttcc
    17761 gtttttcaga agccctgaaa tgtcaaatga tatggcaatg acagcgcctg ttttgataga
    17821 agttttgaat gaatataaaa acttgggaca aaattttgaa tactgcgcct gtatttatcc
    17881 ttgtgcacct tttattaatt ctcaaagact gaaagacgga atgaatcttc tcgttgaaag
    17941 cggagtagac agcgttttgc cgatagtaag attttcttac ccgccacaaa gatgtctggt
    18001 tataagaaat gagaaggttc agatgcttta tcctgaaaat tacaatgtgc gttctcagga
    18061 tttggagcct tattatcacg atgcagggca gttttattgc ctgaagtctt ccagtctgat
    18121 aaaggaagaa aaattatact gcgagaacac tctgccgata attttacctg aaagtgaagt
    18181 tcaagatata gacaacgatg aggattggaa aatcgcggaa atgaaataca aaatgttaaa
    18241 atcgtatttg tagtacaata aaaaataata aaaaggagtt aaaaatggtt tttaagacag
    18301 aacaagaaaa tttttgggcg acagcattcg gtgatgaata tataggcagg aatgatgatg
    18361 atgaattgct tgccggcaat gtaagcatgt tctcaaaagt tttcgcaaaa gccaaaaatg
    18421 taaaaaccgt tcttgaattt ggcgctaaca taggcttaaa tcttaaggct ataaaacaac
    18481 ttcttcccga cgttgaatta tcagggattg aaattaacaa aaaagctgcg gatattttaa
    18541 aagaacagga tgttaaagtt tatcagggat ctattttaga ttttgaagta gattacaaaa
    18601 gggatttttc ttttattaaa acagttttaa ttcatattaa tcctgatgaa ctccaaaatg
    18661 tttaccaaaa gctttatgat tcttcaagca agtatatact gattgccgaa tattataacc
    18721 cgaaacctgt tacgatttca tacagaggac atagcgacag attgtttaaa cgcgactttg
    18781 caggtgaaat gcttgataaa ttcggtgatt taaaattggt ggattacggt tttgcatacc
    18841 atagggacaa aagctgttat caagatgatg taacctggtt tttattggaa aaacaataat
    18901 attaatgtgg gtaaaatatg tataatgctt taattatagg cgcagggcaa attgcaggcg
    18961 ggtatgacag tccgcaggat gagagtgtct tgactcatgc acatgcttac agcaagcata
    19021 aaggctttaa tctgctcggg ttctatgatg ttgatttttc taaagcttct gaaatggcaa
    19081 aaaaatgggg cgtaaaagct tttaaatccc ttgatgaggt tgggagtgtt gatgtaattt
    19141 caatttgttc tcctgacagt ttccatttag cgtcattaaa agagacatta aaattaaatc
    19201 ctaaacttat ttttcttgaa aaacctttgt ctgacaacct ttatgaggca aaagaaattt
    19261 tagaaatctc aaaaaaaatt ccgattttag ttaattattc aagaagattt gtgcctgaaa
    19321 ttcaagaatt ggctaaaacc gctaaaggcg gtgcgttcgg agaattcctt tacggggttg
    19381 gatattacgg gaaagggttt attcacaacg gctcgcatat gacaaatctg cttgatttat
    19441 tggtcggcaa aattaccaaa gtagatattg tacacgaatt tgaagatttt tatcccaacg
    19501 acaagacaaa aaccgcgatt ttatcgtttg attgcaacaa attcactatg aaagggattg
    19561 attgcagaaa ttatacgatt tttgaactcg atttattttt tgaaaaatcc agagttagaa
    19621 ttttagatag cggaaataaa ttagaaattt atgaggtaaa agaaagcgga aattatgcag
    19681 gatacaaaaa tcttatttta aaagaagtga taaatacaga acttaatttg gcaatgctgc
    19741 atgctgttga aaatatttat cgtcatcttg aaaacaatga acctttgaag tctactgtag
    19801 aaaaagcttt tgaggcgata aattatggct aaaataatgt tttttgctca cgatccgggt
    19861 ggtgccaatg ccattttgcc tttaattgag cctttggaga aggaaaatga agtttttgtt
    19921 tttgccaaag gcccggctct tttaaagctg ccgaatccca aaattttgga agaaaacacc
    19981 ctgaaaattg ttaatcctga ctttttgatt accggtacca gcgcaagtga tatgacggaa
    20041 aaattattgt ggcaggattc taaggacttg aacataaagt ccatggcaat tcttgaccac
    20101 tgggtgaatt acgggataag gttttctaaa tacgggctta atgagataga aaaatttgat
    20161 aaaaaatgcg aatttttgcc cgattatata atagtaatgg atgaatttgc caagcaagaa
    20221 atgataaaag acggagttcc tgctgaaatt atttacccgc tgggaaatcc tcattttgaa
    20281 aacttgctta gacagtcaaa aaatgttaaa aatgttcgct caaaatttgc aaaaaaaaat
    20341 gaatttttga taacttttgc atcagaacct tacacggaag attacgggca gggcaatgaa
    20401 aaaaaggttt tagaagattt aattcatatt gccaaagaca aaaatataaa aattattgta
    20461 aaactccatc ctaaagaaga tttttcaaaa tattcggaat ttaaaaacgt aatgtttgat
    20521 aaagaaactg ctccgacaga agttataatg gcaagtgatt tgattgtttc gatgacttct
    20581 atgttcctgc ttgaagcaat gattttgggc aaaaatatat tgagctatca gcctaatgaa
    20641 acaaatcccg ataaatttat tttgaccaga aataaaatgc tcccgtttgt aaaaaataaa
    20701 gaagatttta aggaaaagat acttaatatt ctaaacggga agagatattt gcagtataat
    20761 caaagtatag aatttgatgc cgtagaaaaa attataaaat ttataagtga ggaattatgc
    20821 cggaaataaa aacttcaaaa ttggcgataa acggcggcga aaaaacaaga aaaacaaaat
    20881 ttccgccata tttacctatt ggggaagaag aatttgaaaa tgtaaatgaa gtttttaaat
    20941 caaatacgtt ttcaagattc ttaggctgct ggcacgaaga tttttttggc ggagaacagg
    21001 ttcaggcatt agagaaagag tgggcagagt tttttaaagt taaacatgcg attgccgtaa
    21061 attcagcaac ttccgctcta tattgtgcgg tcggagcaat cgggacagag ccttttgacg
    21121 agattatagt gtcgccctat acaatgtcag ccagtgctac cgcgccattg atttataatg
    21181 caattcctgt ttttgctgat gttgagcctg attgtttctg tcttgatgtt aaagatattg
    21241 agaaaaaaat taccgacaga acaaaagcca taatagttgt tgatatttta ggtcagcctt
    21301 atgatgccga aaaaataaat gttttagcca aaaaaaataa tctgaaggta attgaagact
    21361 gcgctcaagc accttttgca aagtacaacg gaaaatttgc gggaacgctt ggcgatatag
    21421 ggatttattc gcttaattac cacaagcaca ttcactcggg cgagggcgga attttggtta
    21481 caaacgatga tgagcttgcg aacaaattac ggttgataag aaatcatgcg gaggctgttg
    21541 ttgaggctaa aggcgaaaca gattttgtca atatgatagg gtttaattac agaatgacgg
    21601 aagttgaggc tgcaatagct cgcgctcagc ttaagaaatt acctaaattg gttgaagaac
    21661 gtcaaaaaaa tatcgcatat ctggaaaaag aattatcgaa aataccttgt ctgaaaatgc
    21721 ccaaagtcag ggaaagttcc accaatgtgt actaccagca cgggatttta ttcgacgaaa
    21781 aaatcgcagg cgttcacagg gataaattta ccgaagcctt aaaagctgaa ttaatgccga
    21841 taatgttgag ggaaaccgaa ggtataaaag tcggatgcgg gtacgtaaag ccgctttacc
    21901 ttcagccgat ttttcaaaag aaaatcgcat acggttcaaa aggattcccc tggaatatgg
    21961 cggggcggga atatgattat tcgaaaggta tttgtcctgt ggtggaagat ttgcatttca
    22021 acaggctttt aactcatgag tatatgcgac ccggcatgaa aaaagaagac ttggatgatt
    22081 ttataaatgc ggttaaaaaa gtttgggaaa atcgaagtga actaatttaa aaacaggagt
    22141 tttataaaat gactttaaaa cacgatgaaa ttataaaaaa acaaattcaa atttataatc
    22201 aggcaattga aaagtatggt gatgccagcg gtgctgtgct tttgaatgat caacaaagtc
    22261 agtacttcag gtttcatgag ttaacaaaaa actttgattt taattcggat aaaaaaacta
    22321 ttttagatgt aggttgtggt aatgcggaat tgtataaata tttaaatttc caaggttttc
    22381 gaggcaaata tgtaggggtt gatataaatg aaaaattatt aaaacaggca aagtctagat
    22441 ttaaaaatat tgaggtctat aaaaaagata ttatgaaaga aaatattgat acaaattttg
    22501 attatatggt aataagcgga ctttttaatc ttaattgcgg ccaatcactt gattggattt
    22561 ttgaattttt aagcaaactt tttgttcact gtaatgaaat actggctttt aacttaattt
    22621 caacatatgt gaatttccaa aataaagaaa tgttttacgt tgaacccgaa aaaatgtttt
    22681 cattttgtgt tcaacatctt tctcgcagag taactttagc ccacaataac ctgccatata
    22741 attatactgt tttggttcat aaaaatgaaa attggtcatc agttaataaa taaggaaagt
    22801 atgaaaattc aagttaatga aaataaagag atatgggaca attttgtttc tcaatcgcct
    22861 caggggtgta tctttttata ttcaaaattt ctggatagct taaataaaaa atatgaaatt
    22921 ataacttgta ttgatgatgg aggggaagtt cttgccggtt gcgttatttt gtttgacgaa
    22981 aacaaacggc cgataaaaaa tattcatgcg tttacccaat accaaggtct tatattaaaa
    23041 gatttttcag tattatccaa tcacaaaaga atctctaaag aatttaaaat tattgaattt
    23101 tttattgaag aattaacaaa aaaatatcct tcattatgtt tttgccagca ttataatttt
    23161 gatgatatga gagcttttca gtggtacaat tatcatgaaa gggaaaaagg gacttttaaa
    23221 atagatttaa gatattccgg agttttggat ttaacaaaat tcaaatcttt tgatgattat
    23281 ttaatgtcag ttcgtccggt aagaagacag gaatttaata aagtaaaaga tattgaattt
    23341 cgatggataa aagatgaaaa aattttagac ggcattcaca agaaaacttt tgaaagacaa
    23401 aatatagagc gttccgagga agaatctgct ttgctgaaat caataacaaa aaatgcgatt
    23461 gactttgggt acggtaaatt atgtgccgca tattttgaaa acaaacctat ttcatcaatt
    23521 ctttttatat atgataatac tacggcttat tacttgttcg gagcaaatga ccctgaatat
    23581 agaaatttat ttggcggtaa tttccttatg ctgaatatga taagagatgc ttttgaaaat
    23641 gatatagaaa aaattgattt tgtcggagtg aattcgccta acagaggcga ttataaatta
    23701 agttataacg gtgaattgaa accgtatttt attttaaaat tagaggaata agttggcaaa
    23761 agattttgat tataaaggat atagtgaaga aaaactgtta agactttgcg atgaaaccaa
    23821 aagcgacagg ggaattgcaa aatggaaata ttggtctgcg gaagtttatt ctttcggaag
    23881 gcatataaga gattatgctt tttatccttc agcttttacg ctttattgtt ttacggatca
    23941 tggtgcaggc gcttattttg atgaagttgc aaaacacgaa cttgaaagtg atgcgccatg
    24001 tcaattttac cattcgccat tgagtgttga attatttaaa aaagtttcta ataaaccttg
    24061 ctatacatta ttttcgccat ttgtatggta tcgaagaaaa aataaaatag aaaaaatatc
    24121 tgaagcaaaa ggaactcttg ccttccctat tcattcaact cattgcattg acttagcgtc
    24181 tgacatggaa atttatattg agcagctcaa ggcgttgccg gaggaatttc aacctgtaag
    24241 tgtttgtttg catcattgtg atataaataa aggtcagtat aagcttttct ttaaacataa
    24301 tattccggtt tatactgcag gagacatgta tgaccaaaat tttgcccaaa ggttttacga
    24361 tatcctgaaa aactttagat attcaacttc aaatgctata ggttcttaca catattacag
    24421 cgtggaaatg ggaatacctt tttctattta tggagagaag ctggtcctta tgaacaattc
    24481 tgattctaac ctcgaaaaag gagaatgtaa atttcaagaa aacccgcaat atcaaaaaat
    24541 ttataaaatg tttgaaggct taaataaaca aattactcct gagcaaaaag aatttgtaga
    24601 gaaacatttg ggtatttatg atggaataag ccgacttaaa atggcaaaaa ttttgtattt
    24661 attatactta aaaaaaggaa acccgtttaa ggatttatct tatgctttaa aaatgattag
    24721 aagaaaaaga aggattaaaa atgagacttt ttaagaaaaa aagaaaatat acaaacgaat
    24781 ttgaaaaaat atttgaagaa aacaaagtta aaaaaagatt tgtagaacaa atcattgaaa
    24841 ttgaagggta caaattcaaa gtgcctgatg cggtaagctt tgcatatcag ataaaagata
    24901 tattcttgga tgaggtttat aagtttaagc ccaaaacaga agctccttta atatatgatt
    24961 gcggagcaaa tgtcggaacg agtattctat attttaaaag agaatttcct aattcaaaaa
    25021 taaaagcatt tgaggcagat gaaaaaatcc atgaatattt gatagaaaac ataaaagatt
    25081 ttagtgatgt tgaagtttat cataaagctg tctgggtaaa taacgataat ttatgcttta
    25141 attctgaagg ggcagatgcg ggaagcatag taaacaattt tgactgtaaa caaactgtta
    25201 aggcgctcag actgaaagaa cttttgcaaa aagaatctga aatagatttt ctaaaaatgg
    25261 atattgaagg tgcagaaacg gatgttataa ttgattgtgc agaagagtta aaaaaggtta
    25321 aaaatgtgtt catagaatac cattctatag tagataaacc tcaaacattg gataagattt
    25381 tatcaattat aacaaacgca gggtttagat atcatttaca aaatatttct aatcagcagc
    25441 acccatttat tgaaagaaat atttattgta atatggattt gcaggttaat atttttggat
    25501 atagaagtta aaggttataa aaggagttca aattgacaca acaaataccg atttttttag
    25561 caagtgatga taattatgcg ccgtttgtag caaccacaat cacttcaatc gtaaagaata
    25621 caacatcttt tataaatttt tatgttttag acggcggtat tgttgagtgg aataagcaaa
    25681 aaattcaaag tattcaaagt gattttaaaa acttttccgt tgaatttgtt gatatgggca
    25741 agtgcggact ggaaagattc ccgaatttaa atcattactc tgtaaataca ttttcaagat
    25801 attttattcc tgaactaaaa cccgatttag ataaaatttt atacttggat attgacataa
    25861 tagtaaaagg agatattgca cagttataca atcaggaaat gaacgattat cctatagccg
    25921 caattttaga agatttttac cccgagaatt ataaatattt aaaacaaata tgccctaact
    25981 atgatggcgg aagcaattac ttcaatgcgg gagttttatt aattagcctg gattatttca
    26041 gaaaaaataa tataactcag cagttaatcg atacaacagt caaattaaaa gatttgataa
    26101 gatgtgcaga tcaggatatc ttaaattttg tttttgcaaa caattttaaa attcttgatt
    26161 ataaatttga ttttatgccg gaccataaag attacattta taagttaggt aaaaaagaag
    26221 caataggcgc gatagacaat catgttctaa tccattatac cggcagaaaa ccatggaaca
    26281 caatgcaggc ggaaaatgct caagattttt gggatgtggc aaaattaacg ccgttttata
    26341 aagaattgaa aaccgctttg gatataaagt caaaagcaga ttgttatgat gaatatttaa
    26401 aaaaaattgt acttaaaaaa ttaacttgta attataaaag atacaaggtt ttttccaaat
    26461 tatttatagg tggtaaaaga aaaaggtaca aacaaaaagc attagaagtg aaaaacaaaa
    26521 tagatttgtt aaccggtcaa taaagtttta tttcctcaaa aaattaacaa tttcatctaa
    26581 tgctgtttga gcgtcaattt ttgaaataac aaccccttta tcataattct taaccctttg
    26641 cgcaggtgct cctatatcaa aacatgcaac cggatattta agcatcattg cttctgcggt
    26701 tgtgtaagaa aaagtttcgg gccatattga cggaatacac gttttagaac atcttggtgg
    26761 cggagtcgca ccctggaaca tcaacaacta caatttcagt ataaaagaag agattttact
    26821 taaagaaaaa agcactttaa gagagaataa agtaattttt tatcactttc attcggtaaa
    26881 aatagacaaa aagggcagaa ttgacagaaa taagataaat ttttacagag tcacatcaga
    26941 aaatttcaaa atattttata agccctatat taaaaaatta atcgatatga gccgtgattt
    27001 acatcaaatt aatcccgtta ttaaaataat taaaaaacct aaaatagatt ttaaaagtga
    27061 gtttaaaaag ttaagaaaaa aaatcattaa aatacgattt tcttcaaaaa atccggaact
    27121 tattattttt ggtatcagaa ttatataaaa tttgtttatg ataaaatcat tttatgaaaa
    27181 tttctgtaat tactgtttgt tataatgcaa aaaatgacat tgaagacact attcaaagtg
    27241 tattcaaaca aacagctttg gaaaatattg aatatattat cattgacgga aattctacag
    27301 atggcacgaa agaaattatt aaaaagtaca aagataaaat ttctaaatat atatccgagt
    27361 ccgatacggg tatttataat gcgatgaata aaggaattaa acttgcgaca ggggatttta
    27421 tccaattttt gaacgcgggt gatttttata ttaatgaaaa agttgtggaa gactttttac
    27481 cccatttgca aaacgcaaaa agcgaaataa ttttcgggaa tttgtttgca tttaataaat
    27541 ataacggtga tgttgtcaga attaatgaaa atattatcga taaatacttg ttcaattatc
    27601 agtcaatccc gcatccttgc acctttttca ggagagaaat ttttgaaaaa gttggattgt
    27661 ataatgaaaa ttacaggata gtaagtgatt ttgaatggta tttgaaatat ttcaacgaat
    27721 atggcggcag ttatctttat gttgataagt acgctataat ttttgattta aacggaatat
    27781 gttcaagtgc aaaatttgcc gatattcaca gaaaagaacg taaagaagta gtgaaaaaat
    27841 attactcggg acttgagcat tttatatata aagtttcaaa aataatattt aaaagacaac
    27901 ataagaaaaa atatttcaga aaaatgttag caattataaa attgaataaa ttttataaaa
    27961 aagaaagcga aaattaaatt atgccaaaag tttcaataat aattccttgt tacaacagcg
    28021 gcgaatacct tgacgaggca gtagaaagtg ttaaaaatca gactttcaag gatgttgaaa
    28081 ttataattgt caacgatggt tctacggatg taaacaccat tgagcaatta aaaaaatatt
    28141 acaacacaga tgtaaaagtc ataaacacag ataatcaagg agtatctcag gcaaggaaca
    28201 ccggtataaa agaagcaggc ggggaatata ttctcccgct tgattcggat gataaaattc
    28261 atccagaata cgttgagagt gcagtcaaaa tccttgatga aaatctgcaa ataggaattt
    28321 gctactgtga agccgaattt ttcggtgata taaaaggaaa atggaatttg ccaaaatttg
    28381 aattgaaaga ttttttggca agaaactgta ttttttgtac ggctatgtat agaaaatctg
    28441 cttgggaaaa agtcggtggt tataaaaaag aaatggataa ggggtttgaa gattgggaat
    28501 tttggctttc gctgattgaa gcaggggggc aagttcacag aatccccaaa atcttatttt
    28561 attaccgtca atcggagttt agccgctcgg caaattcggc aaataaaaat ctacccgaat
    28621 taattaaaaa aatagtaatg tttcacccaa aactctacgc cgaaaacctc gaatatttgc
    28681 ttattccatg gattgacatt ttttcgaaat caattttaca aaatagtaaa atatacagaa
    28741 taaaagcaaa aatgagcaag aagtttaata ttcttgttat gttagaaaaa agataaaatg
    28801 accgaagcta aaaatataaa aattgcatgt gttttcaatg acaaaaagat ttttgacgaa
    28861 gtagtcgggc aaaatcaaaa cgtcaaagat tttgaaataa caacttttga caataccgtt
    28921 gaaaatatcg gaatttc
//
LOCUS       DAOQAU010000016        28408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1653_length_28408_cov_7.356541, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000016 DAOQAU010000000
VERSION     DAOQAU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 28408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28408
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_28408_cov_7.356541"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKI94_03900"
     CDS             <1..635
                     /locus_tag="PKI94_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25915.1"
                     /translation="KDFEITTFDNTVENIGISKRYNSYLEKLNKDDDFWIIFCHQDFG
                     FEKNPYKKINSLNKKNIYGAIGTSFYYSPIRAIKKFLMKKSDEYSPFKRKFFGQIRQG
                     QGELGFKDFGEKIFFEKSVSTLDCCCMIVHSSLINKYNLRFDENLDWHMYVEDFCINA
                     SKNHKIKLKAAQFNCFHLGEGSLNEDFFNCAKYVKRKYSLKNLFTTCLDG"
     gene            complement(632..1201)
                     /locus_tag="PKI94_03905"
     CDS             complement(632..1201)
                     /locus_tag="PKI94_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNW25916.1"
                     /translation="MEDYIIQSIETKKKILQDKKLLKDIEKMSEVIVETLVNDRKLLF
                     VGNGGSASDCDHLATEFVSKFFKERKAFNAISLASNNALITALSNDFGYEKVFSRQIE
                     AIGKPGDILFAFSTSGKSKNIIEALKTASMMGLIKIGFTGITPCEMDKFCDILIKIPA
                     KETSIIQESHMMLGHVICKMVEDKLFSHV"
     gene            complement(1459..2517)
                     /gene="ugpC"
                     /locus_tag="PKI94_03910"
     CDS             complement(1459..2517)
                     /gene="ugpC"
                     /locus_tag="PKI94_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNW25917.1"
                     /translation="MAEVILKNIQKAYDKKNVIDGIDLEIKDKEFLVLVGASGCGKST
                     ILRMIAGLEEITSGDIYIGERKVNKVAPKDRDIAFVFQSYALYPHMTVYENIAFGLKM
                     RGFSKKVIDEKVKEAAEILDLKSYLDRKPKQLSGGQRQRVALGRAIVREPKVFLMDEP
                     LSNLDAKLRVQMRSEIKKLHQKLQTTFIYVTHDQTEALTMGDRIVVLDKGVIQQIDTP
                     ENIYNDPKNVFVAGFVGSPQMNFLEGKIADNSIKINELSIPMTQAQISAVGDRKKVLI
                     GIRPENMTSEGGKIRFCTKVDMVEMLGSEKIIYFYIGQNRCSVKLPPEFSVDEDLILN
                     FDAKNILIFDKKTNERLY"
     gene            complement(2539..3675)
                     /locus_tag="PKI94_03915"
     CDS             complement(2539..3675)
                     /locus_tag="PKI94_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNW25918.1"
                     /translation="MVFRNFLLKVKDADVLSNLADGVILIDTSGKIQLVNDTACIILN
                     ISKNEFLKANINEIIESGLDLTKQIVENAKSAVGRARTTADKELFLEITGRILDDYYL
                     LSIRDVTQSYKTVAGILTEHEASKKINKDKNAFLVKLSNELKSPVHSILGFSQAMVEG
                     LGGDMSEKQAKYIKIINKNSNDMLYLMDKIVELSKTESNLFEYDFQIFDVINTVQGII
                     KNNEATVKEKNLNMVFDTEDIVKRTIFSDEGALKVILQNILETAIKSTDIGSIKIKAH
                     HPDLEFVFQHDVYVPQDAVEKSYIMFSITDTGIGLNENELAVLFEPYAQLDKPNKKNI
                     VKSIALASAKNLVKTLGGDIWVESESMQGTTYNFIIPAEKFHQE"
     assembly_gap    3762..3861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3862..4374)
                     /locus_tag="PKI94_03920"
     CDS             complement(3862..4374)
                     /locus_tag="PKI94_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNW25919.1"
                     /translation="MNKVLLLNSDCEPLHITSWKRAVVLLIKGKAEYIEKLEDVENYI
                     KIDEYYIPKTIKLTYDVAVPNLELPFSRENIFVRDEHTCQYCGRKFPSSELTLDHVYP
                     KSRLGPDIWENIATCCKECNQKKADRTPKEAGMKLIKRPSRPDDYLLFEISNSKENRE
                     YWEKFYKKAS"
     gene            complement(4518..5588)
                     /locus_tag="PKI94_03925"
     CDS             complement(4518..5588)
                     /locus_tag="PKI94_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNW25920.1"
                     /translation="MKTVEKTKMDEFEKLLEESFSQSYNVADIVEGTVITKEQDGYLV
                     SIKGAKTEAILPNREVPSAAGEENTALEVGDVKEFYVLREENDDEPMLLSLKRIACAQ
                     AWMQLNDAKISGDTILAKVISLVKGGVLVDVADLKGFIPSSQLRTGTPFDGLIDQKIE
                     VKVLEADPKKNKLILSQRQAVADQRDQVVDDVLASLSEDMVVKGEVVRIADFGAFVDI
                     NGIDGLLPISEISWQRIKHPSDIISLGEKIEVKIIKIDNELKRISLSLKRMGENPWQQ
                     IEGEFKEGQIINGTVNKITSFGAFINIFPGVEALLPVAEMSNENVNPFNLFKVGDEVK
                     VLIKKFTPQEHRIALSIKDIQE"
     gene            complement(5575..6456)
                     /gene="ispH"
                     /locus_tag="PKI94_03930"
     CDS             complement(5575..6456)
                     /gene="ispH"
                     /locus_tag="PKI94_03930"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNW25921.1"
                     /translation="MSKNIKLAKHSGFCYGVKRAVETVKKLKEQNPDKNIFILGELIH
                     NSQVIQELEDLGIKTINELPEKGSGICVIRSHGASPEIFEQITKAGFEVVDLTCPDVK
                     KVQKKAIELVKEGYFLIIVGKAEHPEVVAIKANAQRFGADIFVASDVEQLRNIEDKIK
                     EYKKIGVVVQTTQRVTTLKPIVEYLTSISQELSIANTICNSTTLRQNEAKNLAEESDL
                     MIVVGSRKSANTTHLAEILKDISKTIHIEDDKELDNYKNVIKDAKNISITAGASTPEK
                     IINNVIDKLKGEKNENS"
     gene            complement(6545..6754)
                     /gene="yidD"
                     /locus_tag="PKI94_03935"
     CDS             complement(6545..6754)
                     /gene="yidD"
                     /locus_tag="PKI94_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNW25922.1"
                     /translation="MFKKILIKLIEIYQKISSLTPPVCRFSPTCSQYTKEAIQKYGVL
                     KGLWLGTKRVCKCHPFNKGGYDPLP"
     gene            complement(6754..8859)
                     /locus_tag="PKI94_03940"
     CDS             complement(6754..8859)
                     /locus_tag="PKI94_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNW25923.1"
                     /translation="MANPNSGNPISEILLALFVLMLILILLIEMPTGIINFSISLNIT
                     LGIVLLMISLYVQKPLELASFPSIILIGTMFRLVLSIAATRLILSKGDAGEVISAFGH
                     FVTGGNMIVGGVIFLIITVVQFLVITKGAERIAEVAARFALDAMPGKQMTIDADFNAG
                     LISPEEAVKKREDLQRESNLFGSMDGAMKFVKGDTIAGIIIVFINIIGGLCIGTLMNG
                     MPLADAVQKYTLLTIGDGLAAQVPSLLMAISAGIIMTRASAASSSLGSDLAEQIMSKP
                     YSLFFAGLFLFFIAVTAPITGLPWAPFLFFTIVIGVAGVSVLINADVQSQLGQLESVR
                     QNMQDLVNPNRMYERLGVDVLSLQVGSGLLSIADPDQEGQLLAKIAALRQRITDELGY
                     ILPNIRIMDSSALEAEEYLIAIRGNTVATGFVYPKRYMVIADQWDTVRKNPPKDAIVG
                     VDPTYQTQAYWISEEEAKQIRGAVTAVDATDVIVTHLQECVRRHVDDVMTKTDVLKLM
                     ELVRSQDPTLVNDLVPAIISTSDLRKIFVNLIREKVSIKDIMFIFERLCDYARFSKEP
                     DILSERLRAALGRQICLSNVQDDKVLYALTLSPEWEKTLDECCQRTELGTMFLLNPMQ
                     VQELIETTATTLMQSHQSVGKQPVILCSPRVRLPLYQLLERHIPTIVVISYSELITDI
                     KVEAIGTIGESYSTQYAIE"
     gene            complement(8859..9548)
                     /locus_tag="PKI94_03945"
     CDS             complement(8859..9548)
                     /locus_tag="PKI94_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNW25924.1"
                     /translation="MIIKKNKSHNQKTEQKTSGNTAAQIIEEKIDLFDLDNIDFSQRQ
                     ERRRGDRRRGYRRIDERGLVSRAQEEAGAIKETAFQQGYREGLEKAQSDVEKLRISLE
                     EFMKAKQEVFEYVAPDILEIGVDIARKIIKKETQQDPQILLDTVLDILKTISKDESRI
                     TIKVNPAEVAFVKENIAKIIEKTGIDARVSVAAAEETEIGDCIVYTNNGVVDATIDTQ
                     LEIIKEAFKGI"
     gene            complement(9545..10489)
                     /locus_tag="PKI94_03950"
     CDS             complement(9545..10489)
                     /locus_tag="PKI94_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25925.1"
                     /translation="MAIEGFDYQNFAQVLASQVGELLPADFSQEQKNYVTNTVMNFSL
                     LAGEALYKDNDLKFNADQAMLVTQLIAEWSFHKSVDLIRAQLPAEHWDAIMQKIAFTI
                     FEIAKQSIQQGMQQDQILQIVEEQVRKTYSEMLNELKQNNIINDELLDKASSISNIDE
                     MMSKIQEEKMNAQASGQNPDYKILKLASVAIVLKQISQGRALKILNKFSPEDAKAIMQ
                     YMEMPDLEDKVDKQIAVKCFEEIKTILPEPKTVNPNKILNRLRSVIVQKDRVRTEILL
                     RKERPQVRQLVKTAMEGGFLEVPARVVNVVSDYIEENK"
     gene            complement(10557..12194)
                     /locus_tag="PKI94_03955"
     CDS             complement(10557..12194)
                     /locus_tag="PKI94_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HNW25926.1"
                     /translation="MDFQKLLENKPLLYGIIAAVVVVLAFSITMGVISSTKAPSGKTT
                     ENEKIVKEPLDLLTTDNLGKAIEIQALLAREGINADRRLDGTKSTLYLKNYTQTQRDR
                     ALLAIVKSGLMDQNVGLEIFDKGDFTSTKEDKRIRLARAINGELARLIRKIPPIENAS
                     VFISIPEQSMFTSLQKPITATVQVTIPSGEKLDPLKIKAITNLLLGSVTGLISENISI
                     TDTNGNVYNSIMDADDEMLEKIEENDKYMQQKIAVQLDRLIGKGNYVATVSTFLKQNP
                     VEKTSIVYDPESKTAMSEQTFSEGLGDRSQDMSKGTSAVSIYLPNGLPSGSTDSSQNR
                     SYSRVARETQYGISKTQVNEYIKPGMVEDISIAITLEKNSLPPETTLEELKELIASAA
                     SPKVKADDVTIAFADSIEPYLASDKPANLPKPNQTGNPWWLAIAFIAVGLVGGLKHIS
                     GKVKKEQAKQTEELELLREKYQQQDKQLRDVNLKAAELIEKQSQMAQGLIEQQRREVT
                     TPINQKFDNSMVDLRTELESLDDDEAGEKIKSWIEKA"
     gene            complement(12257..12598)
                     /gene="fliE"
                     /locus_tag="PKI94_03960"
     CDS             complement(12257..12598)
                     /gene="fliE"
                     /locus_tag="PKI94_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNW25927.1"
                     /translation="MSSFYPKIDLAGRIQNSRLDMGIQSMPSARLDELSAPGEIKSFK
                     GVMSGLVENLNQEINAPDQLLKDAMSGNGDVDIHDVMTAMAKAELSVNVATQITTKVI
                     QAYDKIMQIQV"
     gene            12866..13045
                     /locus_tag="PKI94_03965"
     CDS             12866..13045
                     /locus_tag="PKI94_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25928.1"
                     /translation="MYNDFTKKDDEFLTPADNSNLYAFLATNYDISDLAGKIYAQNYS
                     KLNIISLRKASSLAV"
     gene            complement(13034..13618)
                     /gene="hisH"
                     /locus_tag="PKI94_03970"
     CDS             complement(13034..13618)
                     /gene="hisH"
                     /locus_tag="PKI94_03970"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNW25929.1"
                     /translation="MITIVDYEAGNLKSICNMLDFLNIKYKVSFDPKEIEEAKKIIFP
                     GQGHFAQAMKNLKEKNLIEPIKNAISNDAKFLGICLGLQVLFEKSAEANGVKGLGIFK
                     GEVKKFTKGKIPQIGWNKIITTPQNTYLSDDYFYFVNSYYVCPQDQTIISSTCDYNIN
                     FAASVEYKNVTAVQFHPEKSSKAGVIFFKKWLNS"
     gene            complement(13933..16596)
                     /gene="ppdK"
                     /locus_tag="PKI94_03975"
     CDS             complement(13933..16596)
                     /gene="ppdK"
                     /locus_tag="PKI94_03975"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNW25930.1"
                     /translation="MTQKRVWLFREGNANMRNLLGGKGANLAEMTNLGLPVPPGLTIT
                     TETCMDYINNGNQMPEGVMEDVKKALADVEYNTGKKFGDASNPLLVSVRSGARLSMPG
                     MMETILNLGLNDSTIQGMINLTQNERFAYDSYRRFLTMFGSVAWEIEREKFEHVLDQV
                     KAREGVKLDTEVSVEGLKSLIPAYKEIIKNETGREFPQDPYVQLQAAIEAVFSSWNIP
                     RAVAYRNHYKIDHNYGTAVNVQTMVFGNMGDDSATGVSFTRNPSTGENKFYGEYLTNA
                     QGEDVVAGIRTPKQIAELETEMPELYKQYVEIAKKLELGYKDVQDMEFTIERGKLYIL
                     QTRNGKRTAAAAVRIAVEMANEGIITKERAIQLVDPYQLYQLLLPSFDLTAKKNSVHL
                     ATGLAASPGAAVGKIVFDTEEAAQRGTAGEKVILVRIETCPDDIHGMIASQGVLTLRG
                     GMTSHAAVVAKGMGKPCVAGCEDLKIDLKNETLTASNGKVFHKNDIISLDGGTGEVME
                     GAVATIIPEMDDKFETLFKWVDEIKRLQVRANADTPADVAKALELGAKGVGLCRTEHM
                     FMDPDRLPWVQKMIIAGTPEARKEALDKLLPMQYNDFYAMFKAMGELPMTVRLLDPPL
                     HEFLPSKEELISEVATLKAKGQDYKEKEDLLHIVENLSESNPMMGLRGCRLGLTYPEI
                     NEMQVRAIFMACCDLKKEGINVKPEIMIPLIGHVNELKTVQTLLERVAKEVMAEKGCD
                     VDYMFGTMIEIPRAALTADQIAEYAEFFSFGTNDLTQMTFGYSRDDAEGKFLTNYVEQ
                     KILARNPFETLDTEGVGQLMQIALDKALPVRPNLKRGICGEHGGDPDSVKFCHKLGLN
                     YVSCSPFRVPQARLAAAQAVVEEGSLAVSHN"
     gene            complement(16675..18450)
                     /locus_tag="PKI94_03980"
     CDS             complement(16675..18450)
                     /locus_tag="PKI94_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25931.1"
                     /translation="MSKKIKNFLITNTNEIIYITFLIFIFLILSITISRHLGNIIIDC
                     GREAYFPQQILKGKLLYKDLFNIFGPLSYQINAIFYKIFSINLSTLRLAGTINAGFIL
                     ILLYSITRFFTTKEISFAISILIMVSCVFNPWIFNYIFPYSYAMVYAFSTFLFSVLFL
                     LLYLKSSKQFFIPLSWFFLGISMTCKYEYVLYLIFLMFLTFILKINKKYILYSLILFL
                     ITPVLSFSVLFLQGLTINDLLEQFRIIKQFALSPSLNYFYSYAVGLYPNKFNMLTNWK
                     FFYNFCILFLLLFSSLYFWVNLSKKSPHYLYKKILSYIFIAVFTYQFFNRLNEYINLL
                     FCWLPLFTLLLLIPLFFKSKKSFSKEFFTTKNGTYILLIMIAMLASAKSFFYLNLKAY
                     ATFAFPLLFIVNSIFIIEFLPLYFKIINKKSLKKAFFIIILIMTILFAGYLKQSLGDD
                     VLIQTSKGSIYNHKNLAESFQASINYIDKEMKKEDSFLVLPEGIMLNFLTNHPSKSIY
                     YATNTPYLEPFGEEKIIDDIKKNPPEYIFINNRNSEDYGKKYICKDYGFDLCNYVKSN
                     YSKIEEFGTHFKMELYKKTSKIICN"
     gene            18527..19735
                     /locus_tag="PKI94_03985"
     CDS             18527..19735
                     /locus_tag="PKI94_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNW25932.1"
                     /translation="MQEFELKNNIKVVCRENKNTPRVALSFNVAINEPEDIAGVYCLM
                     SRLLLQGTKNRTSEQLAQELDENAIELNCDMKQDYLRFRFVCLNEDFEKALEITSDVI
                     KNSTFEEFEKEKIKMQGEITAELDSAKTKALDNYYKTLFEGHFYGHTYTKMLENIDKI
                     SKDKVIKSFEKIISEGKKILAVVGDIKEEEVKNLLNKYFGDIENSNIKEQNISIPVLK
                     EAKTVEIIKSDAQQAQIIQGWIFPTFDSKDYPVLILTNVILGSAGLSSRLFLELRDKK
                     GLAYTVRSSYEPNQKCANFSIYIGTEPKNIQTSIEGFEQEINKLKQIPVGEEELENAK
                     NNLIGKQQFITETNAQQANQLSYYGIMDLGFDYQEKIIQKIKDVTSAQIQECANKYFS
                     DKFIVSILRP"
     gene            complement(19719..20342)
                     /locus_tag="PKI94_03990"
     CDS             complement(19719..20342)
                     /locus_tag="PKI94_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25933.1"
                     /translation="MQNSKVSFTSRIIFTRCEDHIKENKKMYKNLEAGYPCTLKKCVF
                     APSAYTESVRTCTAGGFVINQNGQRKILMFHLDPNELENLKLDNIQKELIQKIGDNAV
                     ERALLMGSKDIKTSKGYENSPLFFNFLENFVQKLNIPYSKLQGLSERDCANLSYDGKS
                     DTWKVSTKFTSYLRKDVFEQKAKKAFEKVLIDKGDEFIIEQNPTGAK"
     gene            complement(20324..21226)
                     /locus_tag="PKI94_03995"
     CDS             complement(20324..21226)
                     /locus_tag="PKI94_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNW25934.1"
                     /translation="MKIQSYSPIYKKADFAKNFTFGWYPNDTLYPKHKNPEKDFSNYT
                     NDIKSYTELVRNPVVFEMLPKYLELYYPDGAVIYDYACSVGHEPCSLVMSLFNYFPQE
                     EAQKYLPIKAIDLREDIIETAKKHSIKIDKNEMLKLTNIFKNIDLEDYLTIEPNSRYA
                     GYGKTFKLTEKLSDNINFQTGDIFADLKDNELSKKPCIVLFRNSWQYLSPDGAQNLTK
                     LLAEKLPDGSTLIVGDKDIYDANVKNLLKKVGFKPTLFGAKSAEELNKEPYFRVDPYG
                     IQHKYLLGDYCFKLQKGGLCKIQK"
     gene            complement(21272..22105)
                     /locus_tag="PKI94_04000"
     CDS             complement(21272..22105)
                     /locus_tag="PKI94_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25935.1"
                     /translation="MKRELIKYFKSLGIEVKTTTKARGHQGFFRQNRIDISKNVKDER
                     IIPTLLHEFAHYIHAKIEPNMPKTGGGVNILFNLSPVHPFTCSALQNELLHVTRFVDE
                     NSHLHKLYEHREQIKAKIKVQENIVKTAYPDFMRSKKFKDFDKYIKKSKAKYLLRYDR
                     VKFVSPFLRRVEIFSVDKLEQDFPDMPAHFCAYIRMKSLQKKQARNSRRINYYNKYYS
                     KPSELFARFVEGLYIDGARVRALAPNTTQRFFELLEAGYYFELKNVLEMLQQNEIIAN
                     S"
     gene            complement(22205..22495)
                     /locus_tag="PKI94_04005"
     CDS             complement(22205..22495)
                     /locus_tag="PKI94_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNW25936.1"
                     /translation="MTKQSYVYIMTNYNETVLYTGITSDLIKRVYEHKNKLREGFTKN
                     YNVTKLVYFECFDNIENAIIREKQIKAGSRNKKLELIKKFNPDWIDLYDKIV"
     gene            22731..23168
                     /locus_tag="PKI94_04010"
     CDS             22731..23168
                     /locus_tag="PKI94_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25937.1"
                     /translation="MKIIPITTFQMFNSTNLPCKKPSFKGVISDVDWANQESKKEQDA
                     FYRTEVYAEHRNEMEGPFICHDGTSYNSKLDAEIQNVRKRLNKLNISEDDFEQLFKTF
                     MEERKINEEIEPNPVKYYRTNGLKPIVAFLEFLEKIKEMTAKN"
     gene            complement(23172..23486)
                     /gene="cutA"
                     /locus_tag="PKI94_04015"
     CDS             complement(23172..23486)
                     /gene="cutA"
                     /locus_tag="PKI94_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNW25938.1"
                     /translation="MEFIVIYCTVPNKKEGKEIAKALVEKKLAACVNIVEKMESVFSW
                     DGEICEEHEALMIIKTRKELFERVRIRIQDMHSYNVPEIIAIPIVEADETYLKWIEHE
                     TK"
     gene            complement(23498..24883)
                     /locus_tag="PKI94_04020"
     CDS             complement(23498..24883)
                     /locus_tag="PKI94_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNW25939.1"
                     /translation="MKFDAAIIDKIKQIVSFENVLETIEERYVYALDATNKQNIETLP
                     GVVVFVETIEQVQKIVRLGYENEIPIVARGAGTNLVGACITTCGGIVLNFSKMNKILE
                     INEQNLTARVQPGVVVGDLQAEVERIGLFYPPDPSNLSVSTIGGSIALCSGGPRTFKY
                     GSTKDYAIDLKVVLADGRILKTGSNTAKNSTGYHMSQFFIGSEGTLGIIVEATFKLIP
                     KPEGSRVILAYFDKISDATNAVTQILKNKLSPATLDFMDKNTIQTIEKFYPSRLLTDK
                     DAVLFLEVDGPIISLDSQQEKIAQFCKQFGASDIRISSNEQEAQKIWTARRSAFGACA
                     KLKPNVVAEDIVVPREKIPQMVEGIRRICTQNNLTVSIVGHIGDGNVHPNISLDLTDE
                     NDVKNYAKTKDEIHQLAIDLGGTLSGEHGIGCEKSKYMTNAIDEVTLEYMGKIKHVFD
                     EKNILNPNKIF"
     gene            complement(24912..25913)
                     /locus_tag="PKI94_04025"
     CDS             complement(24912..25913)
                     /locus_tag="PKI94_04025"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25940.1"
                     /translation="MDNKILFVIDKIEVKYFEFNKLITDFWLIKEFLERNFEVAVTTA
                     DRLCLDNSKAFCKCSKVILKENNIFLDKIWQKQQIQDFTLVMFRPDPPVDLDYINSTY
                     IFDFVDRQKTLIINDTKAIRDFNEKLHANTFFEFMPENLVTSNKEDILEFLEKHDQII
                     LKPLNKCFGVGVMYLKKGDKNINSIINSMTNNQTALTMVQEYISNANYGDKRILTLGK
                     EVLDECVVKLPGNNDFKFNTHSDEYIKKATLTEGEKIKFQKVAKKLNEMGVFMAGLDV
                     IDEKIIEINVTSPCYFIKEINNYFSISLEKKIVDFLVSCLNKKNNELTLSLANKNSL"
     gene            complement(26267..27865)
                     /locus_tag="PKI94_04030"
     CDS             complement(26267..27865)
                     /locus_tag="PKI94_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25941.1"
                     /translation="MIKFNKISQSVAKNAKVFVRDMGKADALLPIILLEAAVTGGRTF
                     HAYKRDGFVEARERFTEETLGAIFWFCGIDFFRSLGDKLGKSVFKLPEYGFDTGEDAI
                     RKPMGNFIMKMKKTAGNVVKDSDKFEKALSKFAFGKVMASVVLANLVVGFVVPKINQK
                     ITKKYLSGKNKEDQALLHEYVSFNDFIEKTKKSNNPAFKGGVTLRNLLSFSYKIENNS
                     TYKLLATDIGVAGGRGISARNKHERIECLFRDLSSIYFYMFCRGHINSVLNFLQDGNK
                     EGIHSFKALFKELKKGRIDPVSVDVFNEHIISKMNPEGISLEEFKVKLLGDEKLVPEM
                     FADDKNYKSIMLDEFINKVKELKLENSDSIIETAKKMAKLQPELPEGAVLSKSQIEDI
                     FKKGLIDEPEFLDKIYTSYYKKGKSIFAKIFNKEAPEEISKHLCDTTFISQEDLLKLK
                     QNVSEYIEILYKKAKSSQKEFIDADFLVKMAKQNFWKNAFNLGIGFVISGYFLSTAIP
                     KIQYWITKKTTGQDKFPGVQKYND"
BASE COUNT         8536 a         5238 c         4524 g        10010 t
ORIGIN      
        1 tcaaagattt tgaaataaca acttttgaca ataccgttga aaatatcgga atttcaaaaa
       61 gatataacag ctatttggaa aaactaaata aagacgatga tttttggatt attttttgtc
      121 atcaggattt cgggtttgaa aaaaatcctt ataaaaaaat caattcgctt aataaaaaaa
      181 atatttacgg tgcaatcggc acaagctttt attacagtcc aatcagggca ataaagaaat
      241 ttctgatgaa aaaatcggat gagtattctc cctttaagcg aaaatttttc ggtcaaatca
      301 ggcaaggtca gggtgaattg ggtttcaaag atttcggaga aaaaatcttt tttgagaaat
      361 ctgtttcgac tctggattgc tgttgtatga tagtacattc atcacttata aataaatata
      421 atctcagatt tgacgaaaac cttgactggc acatgtatgt tgaagatttt tgcataaatg
      481 cctcaaaaaa tcacaaaata aaactgaagg cagcacagtt taattgtttt catctgggag
      541 aaggcagttt aaacgaagat ttttttaatt gcgcaaaata cgtgaagaga aaatattccc
      601 ttaaaaattt gtttaccacc tgccttgacg gctaaacgtg agaaaaaagc ttgtcttcaa
      661 ccattttgca aattacgtgt cccagcatca tatgcgattc ttggataata gaagtctctt
      721 ttgcagggat tttaattaaa atatcacaaa atttatccat ttcacagggt gtaatgcctg
      781 taaagccgat tttgattaac cccatcattg atgctgtttt caaggcttca ataatgtttt
      841 ttgattttcc ggaagtggaa aaggcaaata aaatatcgcc gggttttccg atagcctcaa
      901 tttgtcttga aaaaaccttc tcatagccaa aatcatttga taaagcggta ataagagcat
      961 tatttgaggc aagagaaata gcgttaaaag cttttctttc tttaaaaaat ttagacacaa
     1021 actcagtcgc caaatgatca cagtcgcttg cagatccacc atttcccaca aaaagaagct
     1081 tgcggtcatt gactagagtt tctactataa cctcactcat tttttcgata tcttttaaaa
     1141 gttttttgtc ctgaagtatt tttttctttg tctcaattga ctggataatg taatcttcca
     1201 ttttagctcc ttgctctttg tatctgacat tatatcacat aaaaatttaa gattaaagaa
     1261 gaaaagacaa aaaaaagccg accattcgga cggctactag acttggggag gaggttaaga
     1321 aaaacctttc ggttcttcat acttccttta tcgtcaaatt ctttcaaaaa ctttaaaaat
     1381 ctcatccaaa tggtttacat ttctttatga gctttcagcc cttcacccat taatcttttc
     1441 agctaaatac atttacccct aataaagtct ttcgttggtt tttttatcaa aaatcaaaat
     1501 atttttggca tcaaaattaa gaattaaatc ttcgtcaacc gaaaattcag gcggaagttt
     1561 tacactgcaa cgattttgac ctatataaaa ataaattatt ttttcgctgc ctagcatttc
     1621 aaccatatca acttttgtgc aaaatcttat tttaccgcct tctgaggtca tgttttcggg
     1681 acgaattcca attaatacct tttttctgtc gccgactgca gaaatttgcg cttgagtcat
     1741 aggaattgaa agctcattta ttttaatact gttatctgca atttttcctt caagaaaatt
     1801 catttgcgga gaacctacaa aacctgccac aaaaacattt ttagggtcat tataaatgtt
     1861 ttccggagtg tcaatttgtt gaataacacc tttatccaga actacaattc tatcacccat
     1921 agtcaaagcc tctgtctggt catgggttac atatatgaag gtcgtctgaa gtttttggtg
     1981 aagttttttg atttcagagc gcatttgaac ccttaatttt gcatccaaat tggaaagagg
     2041 ctcatccatc aaaaaaactt taggctctct tacgattgct ctgccaagtg ccacacgttg
     2101 tctttgtccg cctgagagct gcttgggttt tctatccaga taacttttta aatcaagaat
     2161 ttcggctgct tcttttactt tttcgtcgat aacttttttt gaaaaccctc tcattttaag
     2221 cccgaaagct atattttcat aaacagtcat atgaggataa agtgcataac tttgaaaaac
     2281 aaaagcaata tctctgtctt ttggggcaac tttgtttact tttctttctc ctatgtaaat
     2341 gtcacctgaa gttatttcct caagccccgc tatcatcctt aaaatcgtgg actttccgca
     2401 tcctgaggcc cctaccagca ccaaaaattc tttgtctttt atttccaaat caattccgtc
     2461 tatgacgttt tttttgtcat aggctttttg aatgtttttc aaaattactt ctgccatttg
     2521 atgcctctgt taaaatttct attcttgatg aaacttctca gccggaatta taaaattgta
     2581 agttgttccc tgcatagatt cagattcaac ccaaatgtct ccgccaagag tttttacaag
     2641 attttttgcg cttgcaagag cgattgattt tactatgttc tttttatttg gcttatcaag
     2701 ctgagcataa ggctcaaaaa gaaccgccag ttcattttcg ttaagcccaa ttcctgtatc
     2761 tgttattgaa aacattatgt aagatttttc gactgcatct tggggcacat aaacatcgtg
     2821 ctggaataca aattccaaat caggatgatg cgctttaatt ttaatcgagc ctatatctgt
     2881 tgatttaatt gcagtttcta aaatgttttg caaaatcact ttcaatgcgc cttcatcgga
     2941 aaaaatagta cgctttacaa tgtcttccgt gtcaaatacc atattaagat ttttttcttt
     3001 aaccgtagct tcgttatttt tgattatccc ttgaactgta ttgattacat caaaaatctg
     3061 aaaatcgtat tcaaaaagat ttgattctgt ttttgaaagt tcgactattt tgtccataag
     3121 atagagcata tcgttggaat ttttatttat aatttttata tattttgcct gtttttcgct
     3181 catatctccg cccaagcctt ccaccatagc ctgcgaaaag cccaaaatcg aatgcacagg
     3241 agatttaagc tcgttcgaaa gcttaaccaa aaaagcgttt ttgtctttgt ttattttttt
     3301 tgaggcttca tgttctgtga gaataccggc gacggttttg tagctttgag ttacatcccg
     3361 aattgaaagc agataataat cgtctaaaat tcttcccgtt atttcaagaa aaagttcttt
     3421 atcggcagtt gttcttgccc ttccgactgc ggattttgca ttttctacaa tctgttttgt
     3481 taagtcaagt ccgctttcga ttatttcgtt tatattcgct tttaaaaatt cgttctttga
     3541 gatgtttaaa ataatacacg cagtatcgtt tacaagttga attttgcccg aagtatcaat
     3601 aagaattact ccgtctgcaa ggtttgacaa tacatcagcg tctttcacct ttaataaaaa
     3661 attcctgaaa accatttttt atgccctatt ctaactaaca agaatatatt agcataaaat
     3721 ttgatttttc atatacacta caataaggat tttattaaaa gnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn ntcaagaagc ttttttgtag aatttttccc aatactctct
     3901 gttttctttt gagttggaaa tttcaaacaa aaggtaatca tcaggccttg aaggtctttt
     3961 aataagcttc attccggctt ctttgggtgt tctgtcagct tttttctggt tgcactcctt
     4021 acagcaggtt gcaatgtttt cccaaatatc aggtccgagt cttgattttg gataaacatg
     4081 atctaaggtc aattccgatg aagggaattt tcttccgcaa tattgacagg tgtgttcatc
     4141 tcttacaaaa atgttctcac gtgaaaatgg cagttccaaa ttcggaactg ccacatcata
     4201 agttaatttt attgttttag gaatgtaata ctcgtctatc ttaatataat tttctacatc
     4261 ttcaagcttt tctatatact ctgccttgcc cttgattaaa agtactaccg cccgcttcca
     4321 gctcgttata tgaagcggct cgcagtcact attcagcaaa agaaccttat tcattttctt
     4381 attctcgctt gcagaaaaat tgtttacact tttattattc cactttgggc ttaaattata
     4441 accgctgttt gaagtataaa gggcgtaatg ttaagaaaac ttaacattgc gcccttttat
     4501 tatattcatt gaattgttta ttcttgaata tcttttatgc tcaaagcaat tctgtgttct
     4561 tgcggagtga attttttaat taaaactttc acttcgtcac cgactttgaa caaattaaac
     4621 ggattaacgt tttcattgct catttctgca acaggaagaa gggcttcaac acctgggaat
     4681 atattaataa aagcaccgaa acttgtaatt ttatttacgg ttccgtttat aatctgtcct
     4741 tctttaaatt ctccttcgat ttgctgccaa gggttttcgc ccattctttt taagcttaag
     4801 ctgatacgtt taagttcgtt atcaattttg attattttaa cttcgatttt ttcacccaaa
     4861 gaaataatat ctgacggatg tttaattctc tgccaagaaa tttctgagat aggaagaagc
     4921 ccgtcgattc cattgatgtc aacgaaagct ccgaaatctg ctattcttac gacttcacct
     4981 tttacaacca tatcttcgct caaagaagca agcacatcgt caacaacctg atctctttga
     5041 tcagccaccg cttgtctttg agaaaggatt aatttatttt tcttaggatc ggcttcaaga
     5101 actttaacct ctattttttg atcaataagc ccatcaaaag gcgtccctgt tctaagttgg
     5161 cttgaaggaa taaatccttt aaggtctgca acgtcaacca gaaccccgcc tttaacaagg
     5221 gatataactt ttgcaagaat cgtatcgccc gaaattttag catcgttaag ctgcatccaa
     5281 gcttgtgcac aagcaattct ttttaatgac aaaagcatcg gctcatcatc gttttcttct
     5341 cttaaaacat aaaattcttt tacatcacca acttctaaag ctgtattttc ttcacctgcg
     5401 gcagaaggaa cttcacgatt aggcaaaatc gcttctgttt tagcaccttt tatgctgacg
     5461 agatagccgt cttgttcttt tgtaattact gttccttcaa caatatcagc cacattgtag
     5521 ctttgcgaaa aggattcttc cagcaacttt tcaaattcgt ccatttttgt tttttcaact
     5581 gttttcattt ttttctcctt ttaatttatc tataacatta ttaataattt tttcaggggt
     5641 tgaagcaccc gcggtgattg aaatattttt tgcatctttt atgacatttt tgtaattatc
     5701 aagctctttg tcatcctcaa tgtgaatagt ctttgaaatg tctttaagaa tttctgcaag
     5761 gtgagttgta tttgcacttt ttcttgaacc cacaacaatc ataaggtcag attcttcggc
     5821 aaggtttttt gcttcatttt gtcttaaagt tgtgctgttg cagatagtat ttgcaatgga
     5881 aagctcttgt gaaattgaag tcaaatattc aactataggt ttaagcgttg taaccctttg
     5941 cgttgtttga acaaccactc caattttttt gtattcttta attttgtctt caatatttct
     6001 tagctgctcc acatcacttg ccacaaaaat atcagcaccg aatctttggg catttgcttt
     6061 gattgcaaca acttcaggat gctcagcctt gcccactatt atcaaaaaat atccttcttt
     6121 tacaagttca atggcttttt tctgaacttt ttttacgtca ggacaggtta aatctactac
     6181 ttcaaatcct gcttttgtta tctgttcaaa aatttcaggt gaagccccat ggcttcttat
     6241 aacacaaatt ccgcttcctt tttcgggaag ttcgtttatg gttttgattc ccaaatcttc
     6301 aagttcttga ataacctgag aattgtggat aagttcgcca agtataaaaa tatttttatc
     6361 ggggttttgt tcttttaatt ttttaacggt ttcgaccgcc cttttcaccc cgtaacaaaa
     6421 gcctgagtgt tttgctaatt ttatattttt cgacaattaa atttttcttc tttcaagtga
     6481 actagcagtt tcccgcagtt attacattac agcacagaca gaaaaaaatt gcaagggagg
     6541 gtgtttatgg aagcgggtca tatccgcctt tattaaacgg atgacactta caaacccttt
     6601 ttgtgccaag ccaaagtcct tttaaaacgc cgtatttttg aatcgcctct tttgtatatt
     6661 gagagcaagt aggactaaac ctgcaaacgg gaggagtcag gcttgatatt ttctgataaa
     6721 tttctattaa ttttattaaa atctttttaa acattattca atagcatatt gagtcgagta
     6781 actttcacct attgtgccga ttgcctcaac cttgatatct gttatcaatt cagagtaaga
     6841 aatcactaca atagtcggaa tgtgtctttc aagaagctga tataaaggaa gccttaccct
     6901 tggagaacac aaaataaccg gctgtttgcc gacgctttga tgtgattgca taagtgtagt
     6961 tgctgtggtt tcaattaact cctgcacctg catggggttt aacaggaaca tagttccaag
     7021 ctctgtcctt tggcaacatt catctaaagt cttttcccac tcgggagaaa gtgtaagtgc
     7081 gtataaaacc ttatcgtctt gaacattaga aagacaaatt tgtctgccca gtgccgccct
     7141 cagcctttcg gataaaatat cgggttcttt tgaaaatctt gcataatcgc aaagtctttc
     7201 aaaaataaac attatatctt ttatggaaac tttttctctt ataagattaa caaatatttt
     7261 tctcaaatcg cttgtagaaa ttatggcagg aacaaggtca tttacaagtg tcgggtcttg
     7321 agaacgaacc aattccatta actttaaaac atctgttttt gtcataacat catcaacgtg
     7381 tcttctgaca cattcttgaa ggtgtgttac tataacatct gttgcatcaa ctgcggttac
     7441 cgcccctctt atttgttttg cttcttcttc tgatatccaa taggcttgtg tttgataagt
     7501 cggatcaact cctacaattg catcttttgg gggatttttt ctgaccgtat cccattgatc
     7561 ggcaattacc atataacgtt ttggatatac aaagcctgtt gcaactgtat ttcctcttat
     7621 tgcaatcaaa tattcttcag cttcaagagc agaggaatcc ataattctta tattaggtaa
     7681 aatatatccg agctcatctg ttattctctg cctcagcgcg gcaatttttg caagaagctg
     7741 accttcttga tcgggatctg caatcgataa caatcctgag cccacttgca ggcttagaac
     7801 atcaacgccg agtctttcgt acattctgtt tggattaacc aaatcctgca tgttctgcct
     7861 tacgctttca agctgaccca gttgagactg aacatcagca tttataagaa ctgatactcc
     7921 tgcgactcct attacgatag taaaaaataa aaacggagcc cacggaagcc ctgttattgg
     7981 ggctgtaaca gctatgaaaa acaaaaacag ccctgcaaaa aataaactgt aaggcttact
     8041 cataatttgt tcagccaaat cagaacccaa agaagaactt gcagcagatg cccttgtcat
     8101 tataataccg gcagatattg ccattaaaag ggaaggaact tgtgcagcaa gtccgtcacc
     8161 tattgttaat agcgtatatt tttgtaccgc atctgcaagg ggcatcccgt tcatcaatgt
     8221 tcctatgcaa agcccgccta taatgtttat aaatacaatg ataatacccg caatggtatc
     8281 tccttttaca aacttcatcg caccgtccat tgaaccgaaa aggtttgatt ctctttgaag
     8341 gtcttctctt ttcttaacgg cttcttcggg tgaaatcaat cctgcattaa aatcggcgtc
     8401 aattgtcatt tgcttaccag gcatggcatc taaggcaaat cttgctgcaa cttctgcgat
     8461 acgctctgca ccttttgtaa ttaccaaaaa ttgtacaact gttatgatta aaaatattac
     8521 gccgcctaca atcatgttgc cgccggttac aaagtgcccg aatgccgaaa tcacctcacc
     8581 ggcatctcct ttggaaagaa taagtcttgt tgctgcaata cttaaaacca acctaaacat
     8641 ggttccgata aggattattg aaggaaaaga agcaagttcc agaggttttt gcacatataa
     8701 agaaatcatt aaaagcacaa ttcctaaagt tatattcaag cttatggaaa agtttattat
     8761 acctgtgggc atttcaataa gcaaaataag tatcagcatt aatacaaata atgccaacaa
     8821 tatttcactt ataggatttc ctgaatttgg gtttgccatt atattccctt aaacgcttcc
     8881 tttattattt ccaactgggt gtcaatggtt gcatcgacaa ccccgttatt tgtgtaaact
     8941 atacaatcgc ctatttctgt ttcttctgcg gcagctacgc tgactcttgc atcaattcct
     9001 gttttctcaa taatttttgc aatattttcc ttaacaaaag caacttctgc ggggttaact
     9061 tttattgtaa tcctactctc atccttggat atggttttta atatgtccaa aaccgtatcc
     9121 aaaagaattt gaggatcttg ctgtgtttct tttttaataa tttttcttgc tatatcaaca
     9181 cctatttcca aaatatccgg tgctacatat tcaaaaacct cctgcttggc tttcataaat
     9241 tcttcaagag aaattcttaa tttttcgaca tcactttgtg ctttttcaag cccttctctg
     9301 taaccttgct gaaaagcggt ttcttttatt gctccggctt cttcttgcgc ccttgaaaca
     9361 agccctcttt catcaattct tcgataaccc cttcttctgt cgccacgcct tctttcctgc
     9421 ctttgagaaa aatctatatt gtccagatca aataaatcta ttttttcttc aataatttga
     9481 gcagcggtat ttcccgaagt tttttgttcg gttttttgat tatgagattt atttttcttt
     9541 ataatcattt gttttcctca atataatcag aaacaacatt tacaaccctt gcaggaacct
     9601 ccaaaaatcc accctccata gccgttttta caagttgcct gacttgcggt ctttcctttc
     9661 ttaaaagtat ttctgttctt actctgtctt tttgcacaat aacacttctg agacgattta
     9721 aaattttatt cggattaaca gttttaggct caggaagtat cgttttaatt tcttcaaaac
     9781 attttactgc aatttgctta tcaaccttgt cttccaaatc aggcatttcc atatattgca
     9841 ttattgcttt tgcatcttcg ggagaaaatt tatttaaaat tttcaaagct ctgccttgtg
     9901 aaatctgctt taaaacaata gcaacagaag caagttttaa aattttataa tccggatttt
     9961 gcccagaagc ctgagcattc attttttctt cctgtatttt tgacatcatt tcatcaatat
    10021 ttgaaatgga agaagcttta tccaaaagct catcgtttat tatattattt tgcttaagtt
    10081 cattaagcat ttcgctgtaa gtttttctaa cctgctcttc aactatttgc aaaatctgat
    10141 cttgctgcat tccttgttga attgattgtt tggcaatctc aaaaatggta aaggcgattt
    10201 tttgcattat agcatcccaa tgctctgcag gaagttgtgc ccttataaga tcaaccgact
    10261 tatgaaaaga ccattctgca atcaactgcg ttacaagcat cgcctgatcg gcattaaatt
    10321 tcaaatcatt atctttgtaa agggcctctc cggcaagcag agaaaaattc attacggtat
    10381 ttgtgacata atttttttgc tcctgactaa aatcagccgg caataattca ccgacctgag
    10441 aagcaagcac ttgagcaaaa ttttgataat caaatccttc tatcgccatt tttaattttt
    10501 ccctcttccc ctgttataaa acttctattt actatttacc atttactatt tacttactac
    10561 gccttttcaa tccagctttt tattttttca ccggcttcat catcgtccaa gctttcaagt
    10621 tcggttctca aatccaccat tgagttatca aatttttgat ttataggtgt tgttacttcg
    10681 cgccgttgct gttcgattaa tccttgagcc atttgtgatt gtttttcgat taattctgca
    10741 gctttaaggt taacatctct aagctgtttg tcctgctgct gatatttttc tctcaaaagt
    10801 tccaattctt ctgtttgttt tgcctgttct tttttgacct tgcctgaaat atgtttcagt
    10861 ccgccaacca atcctactgc aataaatgct attgcaagcc accaaggatt tcctgtttgg
    10921 ttaggttttg gcaagtttgc gggtttatcg gatgcaagat acggctctat agaatctgca
    10981 aaagcgattg tgacatcatc ggctttaacc tttggacttg cagcacttgc aataagttct
    11041 ttaagttctt caagtgtggt ttcgggcgga agcgaatttt tttccaaagt aatagctatg
    11101 gaaatatctt caaccattcc cggctttatg tattcattaa cctgagtttt tgaaatcccg
    11161 tattgggttt cccttgcaac tcttgaataa cttctgtttt gagaagaatc tgtgctgcct
    11221 gaaggcaaac cgtttggaag gtaaatgctt accgcacttg ttcctttgct catatcctga
    11281 gatctgtctc ccaaaccttc ggaaaaagtt tgttcagaca ttgcggtttt gctttcggga
    11341 tcatatacta tacttgtttt ttcaacagga ttttgtttta aaaaagtact tacggtcgca
    11401 acataattgc cttttccgat aagtctgtca agctgtacgg caattttttg ctgcatgtat
    11461 ttatcgtttt cttcaatttt ttcaagcatt tcatcgtcag cgtccattat gctgttataa
    11521 acattcccgt ttgtgtcggt tattgaaata ttttcagaaa ttagccctgt aacgctacct
    11581 aaaagcaaat tagtaatagc cttaatttta agcgggtcaa gtttttcgcc gcttggaatt
    11641 gtaacctgaa cggtcgcggt tataggcttt tgtaaagatg taaacatcga ttgctcggga
    11701 attgaaataa acacagacgc attttcaata ggcggaattt ttcttataag tcttgcaagt
    11761 tcgccgttaa ttgctcttgc aagcctaatt cttttatctt cttttgttga agtaaaatca
    11821 cctttatcaa aaatttccag ccccacgttt tggtccataa gcccgctttt tactatcgcc
    11881 aaaagtgctc tgtccctttg cgtttgtgta taatttttca aataaagagt tgattttgtt
    11941 ccgtcaagcc ttctatcggc gttaattcct tctcgtgcaa gaagtgcctg aatctctatt
    12001 gcttttccta aattatcagt tgtaagaaga tcaagaggct cttttacaat cttttcattc
    12061 tcggtagttt tccccgaagg ggcttttgtc gaagagataa cacccattgt aatgctaaag
    12121 gcaagcacaa caacgacggc agcaattatg ccataaagta aaggtttatt ttccagtaat
    12181 ttttgaaaat ccattaaaat ttatcccctg cagtattata ctattttttt aggcttatgc
    12241 ctatatgtta acaagtttac acctgaatct gcataatttt gtcatacgct tggatgactt
    12301 ttgtcgtaat ttgtgtggca acgttaacgc tcaactcggc ttttgccata gcagtcatta
    12361 catcgtgaat gtcaacatca ccgtttccgc tcatcgcatc cttgagaagc tggtcaggcg
    12421 cgtttatttc ctgattgagg ttttccacaa gccccgacat tacgccttta aagcttttta
    12481 tttcacctgg cgcactaagt tcatcaagtc ttgcagaagg cattgactga atacccatat
    12541 ccagccttga gttctgtatt cttcccgcta aatctatctt tggataaaag cttgacataa
    12601 ttttcacctt ccttttgtta tgatttttat ctcggttttt ttaaaactaa agtttatcaa
    12661 ttttggacaa aaatcatccg tttgatgtag gttaaaattt aaaaatcata caaacagtgt
    12721 agtctgcaaa agtaatttta attatttttc aatcaaaatc actttttcgt taacataatt
    12781 taatattcaa aataaaaaag caattaattt ttgaaaaaat actttttatt attataaaca
    12841 cgattatgaa ttaattgagg aaataatgta taacgatttt acaaaaaaag atgatgaatt
    12901 tttaactccc gcagataact ccaatttgta cgcgtttctt gccacaaatt atgatataag
    12961 tgatttggcg ggcaaaattt atgcacaaaa ttacagcaaa ttaaacatca tcagccttag
    13021 aaaggcttcc agcttagctg tttagccatt ttttgaagaa tatcactcct gcctttgagc
    13081 ttttttcggg gtgaaattgt actgcggtaa catttttgta ctctacggat gccgcaaaat
    13141 ttatattata atcgcaagtc gaagaaatta ttgtttgatc ttgggggcac acataataag
    13201 aatttacaaa gtaaaaataa tcgtctgata aataagtgtt ttgaggggtt gttataattt
    13261 tattccagcc gatttgaggg attttgcctt ttgtgaattt tttgacttca cctttgaata
    13321 ttcccaaacc ttttacacca tttgcctcgg cacttttttc aaaaagcacc tgcagcccca
    13381 agcaaatacc caaaaatttt gcatcatttg aaatagcatt tttaataggc tcaataagat
    13441 ttttttcttt aagatttttc attgcctgag caaaatgccc ttgtccgggg aaaataattt
    13501 tttttgcctc ttcaatttct ttaggatcga aagaaacttt atattttata tttaaaaaat
    13561 cgagcatatt gcatatgctc tttaaatttc cggcttcgta atcaacaata gtaatcataa
    13621 gtttatttta tcagtaaatg ggaaatagta aatagtaaat atataacgtc atgctgaact
    13681 cgtttgactt cttttcccga aatctttgat ttcgtggaaa atgtccttac agattatgtg
    13741 gattatccac tctttaggtt taattttcaa tagtatcagc atctattcag cggtatttgt
    13801 tggaaagatc ctgaaacaag ttcaggatga cgaggagatg aacaattttt tcataaaaat
    13861 aaggcgggat ttcatcccgc cttattctat ttactattta ccatttacta tttacttcaa
    13921 tggcatttga ttctagttgt gagaaacagc aagtgagcct tcttcgacaa ctgcttgtgc
    13981 agcagctaat cttgcctgtg gcactctgaa cggtgagcat gatacgtaat tcaaaccgag
    14041 tttgtggcag aatttaaccg aatcagggtc gccgccgtgt tctccgcaga ttccgcgttt
    14101 caagtttggt cttacaggta atgctttgtc caacgcaatt tgcattaatt gtcctacgcc
    14161 ttctgtgtcc aaagtttcaa acgggtttct cgccaagatt ttttgctcaa cgtaatttgt
    14221 taagaattta ccttcagcat catctcttga ataaccgaat gtcatttgag ttaagtcatt
    14281 cgtaccgaat gagaagaact ctgcgtattc agcgatttga tctgctgtaa gtgccgcacg
    14341 aggaatctca atcattgttc cgaacatata gtcaacatcg cagccttttt ctgccataac
    14401 ttcttttgca acacgctcaa gaagtgtttg aaccgtttta agctcgttga cgtgtccgat
    14461 tagaggaatc ataatttcag gtttaacgtt aattccttct tttttcaagt cgcagcaggc
    14521 cataaatatt gctctaactt gcatttcatt gatttcagga taagttaagc ccaatctgca
    14581 acctcttaac cccatcatag gattagattc agataagttt tcaactatat gtaaaaggtc
    14641 ttctttttct ttataatctt gacctttagc tttcaaagta gctacttcag agattaattc
    14701 ctcttttgaa ggtaagaatt cgtgaagcgg agggtcaaga agtcttactg tcataggaag
    14761 ttcgcccatt gctttgaaca ttgcgtaaaa gtcgttatat tgcataggaa gcaatttatc
    14821 caaagcttct tttctagcct caggagttcc tgcgataatc attttttgta cccagggtaa
    14881 tctgtcagga tccatgaaca tgtgctctgt tctgcataaa cccacaccct tagcacctaa
    14941 ttccaaagct tttgcaacgt cagcaggtgt atcggcgttt gctcttacct gaagtctttt
    15001 gatttcatca acccatttga acaatgtttc aaatttatca tccatttcag gaatgattgt
    15061 tgcaacagca ccttccataa cttcgcctgt tccgccgtct aaagaaataa tatcgttttt
    15121 gtggaacact tttccgttag atgccgtcaa tgtttcgttt ttaagatcga ttttcaagtc
    15181 ttcgcaacct gcaacgcatg gtttgcccat accttttgca acaaccgcag catgcgatgt
    15241 cataccgcct ctgagtgtta agacaccttg agaagctatc atgccgtgaa tatcgtcagg
    15301 acaagtttct attcttacca agataacttt ttcgcctgcc gttccgcgtt gtgccgcctc
    15361 ttcagtatca aatacgattt taccaaccgc agcacccggg gaagccgcaa gccctgtagc
    15421 caaatgaacc gaatttttct tggctgttaa atcaaatgaa ggcaacaata attggtataa
    15481 ttgataagga tcaaccaatt ggattgctct ttcttttgtg ataatgccct cattggccat
    15541 ttcaacggca attcttacag ctgctgcagc tgttcttttg ccgtttcttg tctgaaggat
    15601 gtataatttg cctctttcga ttgtaaattc catatcctga acgtctttgt agccaagttc
    15661 aagttttttg gcgatttcaa cgtactgttt gtataattcg ggcatttcag tttccaattc
    15721 agcgatttgc tttggagttc tgatacctgc aacaacgtct tcaccttgtg cgtttgtcag
    15781 atattcaccg tagaatttgt tttcgcccgt ggatgggttt ctggtaaaag atacgcctgt
    15841 ggcagagtcg tcacccatgt taccgaatac cattgtttga acgtttactg ccgtaccgta
    15901 attgtggtca attttatagt ggttacggta tgcaaccgcg cggggaatgt tccaagaact
    15961 gaatactgct tcaattgctg cttgcaattg aacataagga tcttgaggaa attctcttcc
    16021 ggtttcattt ttgataattt ctttataagc aggaatcaaa gattttaaac cttcaacaga
    16081 aacttctgtg tcaagcttaa cgccttctct tgctttaact tggtctaaaa cgtgttcgaa
    16141 tttttctctt tcgatttccc atgcaactga gccgaacata gttaagaaac ggcgatagct
    16201 atcataagca aatctttcgt tttgtgtaag gttaatcatg ccttgaatag ttgaatcgtt
    16261 taaacctagg tttaaaatag tttccatcat accgggcata gaaagtcttg cccctgaacg
    16321 cactgaaacc aaaagcggat ttgaagcatc tccgaatttt ttgcctgtgt tgtattcaac
    16381 atctgcaagt gcttttttaa catcttccat aacgccttcg ggcatttggt tgccgttatt
    16441 gatgtaatcc atgcaagttt ctgttgtgat tgttaatccc gggggaaccg gtaaccctaa
    16501 gtttgtcatt tcggctaaat ttgcgccctt tccaccaaga agattacgca tatttgcatt
    16561 accttccctg aaaagccata ctctcttttg tgtcattttt tgccttcttt cttatttatc
    16621 taaaataacg ttgtaaacat tgtacatgtt accaatagcc taacacaaac aaaatcaatt
    16681 acaaattatt tttgaagttt ttttatataa ttccatttta aaatgggtgc cgaattcttc
    16741 aatttttgaa taatttgatt ttacgtagtt gcataaatca aatccataat ctttgcaaat
    16801 atattttttg ccgtaatctt cagaatttct gttgtttata aaaatgtatt cgggaggatt
    16861 ttttttaata tcatctataa ttttttcttc cccgaagggt tctaggtatg gagtgtttgt
    16921 tgcgtaatag atactttttg aggggtgatt tgttaagaag ttgagcatta tcccttccgg
    16981 aagaactaaa aatgaatctt cctttttcat ttctttatcg atgtaattta tgctggcttg
    17041 aaaactttca gcaagatttt tatgattata aatcgagcct tttgatgttt gtattaaaac
    17101 atcatcccca agagattgtt ttaagtatcc tgcaaataga atagtcataa ttaatataat
    17161 aataaaaaaa gcctttttta aagatttttt atttatgatt ttaaaataaa gtggcaaaaa
    17221 ttcaattatg aaaatgctgt ttacaataaa aagtaaggga aaagcaaatg ttgcgtaggc
    17281 tttgaggttt aagtagaaaa aagattttgc tgatgccaac atagcaatca ttattaataa
    17341 aatataagtc ccgtttttgg ttgtaaaaaa ttctttagaa aatgactttt ttgacttaaa
    17401 aaacaatgga attaatagta ataatgtaaa taaaggaagc cagcaaaata ataaatttat
    17461 atattcgttt agtcggttaa aaaattgata ggtaaaaaca gcgataaata tataactaag
    17521 tattttttta tataaatagt gaggagattt ttttgataaa ttaacccaaa aataaagtga
    17581 ggaaaataaa agtaaaaata atatacaaaa attgtaaaag aatttccaat ttgttaacat
    17641 attgaacttg ttcggataaa gtcctactgc ataagaataa aaataattta atgaaggaga
    17701 tagggcaaat tgtttgataa ttctaaattg ctccaacaaa tcatttattg ttaaaccttg
    17761 taagaaaagt acagaaaaag ataaaactgg ggtgattaaa aataaaatga ggctgtataa
    17821 aatatatttt ttgtttatct ttagtataaa tgttagaaac attaggaaaa tcagatataa
    17881 aacatattcg tatttacatg tcattgaaat cccaagaaaa aaccagctta gcggaataaa
    17941 aaattgtttt gaagacttta aataaagcaa taagaataaa actgaaaata aaaaagtact
    18001 gaaggcatat accatcgcat aagaatatgg gaaaatgtag ttaaaaatcc atgggttaaa
    18061 tacgcaagaa accatgataa gaatactaat agcaaaagaa atttcttttg tcgtaaaaaa
    18121 tcttgtaata gaatataata aaattaaaat aaatcccgca tttattgttc ctgcgagtct
    18181 gagagtgctt aaatttattg aaaaaatttt gtaaaaaatc gcatttattt gataagataa
    18241 aggaccgaaa atattaaata aatctttgta aaggagcttg cctttgagta tttgttgggg
    18301 gaaataagct tctcttccgc agtctataat tatattcccc agatgtcttg aaattgttat
    18361 tgacagtatt aaaaaaataa atattagaaa agtaatatag attatttcat tagtgtttgt
    18421 aattaaaaaa tttttaattt ttttactcaa gttattgatt tccttgcttt atagacagta
    18481 tattaaatca ttgtacatta aatatttttt atgattaaat aattttatgc aagaatttga
    18541 attaaaaaac aatataaaag tcgtatgcag agaaaataaa aatacgccaa gagtggcttt
    18601 gagctttaat gttgccataa atgagcctga agatattgca ggagtttact gtctgatgag
    18661 cagactgctt ttgcagggga caaaaaaccg aacctccgaa cagttggcgc aggaattgga
    18721 tgaaaatgca atcgaactta actgtgacat gaaacaggat tatctgcgat ttcgttttgt
    18781 atgcctgaat gaagattttg aaaaagcact tgaaataacc tctgatgtga ttaaaaattc
    18841 gacttttgaa gaatttgaaa aagaaaaaat caaaatgcaa ggcgaaatca cagcagagct
    18901 tgattctgca aagacaaaag ctttagacaa ttattacaaa accctctttg aaggacattt
    18961 ttacggacac acttatacaa aaatgcttga aaatattgat aaaatctcaa aagataaggt
    19021 aataaaaagt tttgaaaaaa taatttcaga aggcaaaaag attttagcag ttgtgggcga
    19081 tataaaagaa gaagaagtca aaaacctttt gaataaatat tttggggaca ttgaaaattc
    19141 aaatataaaa gagcaaaata tttccatacc tgttttaaaa gaagcaaaaa ctgttgaaat
    19201 aataaaaagt gacgcccaac aggcgcagat tattcagggt tggattttcc ccacttttga
    19261 cagcaaagat tatcctgtat taattctcac aaatgtaatt ttgggctctg caggtctttc
    19321 ttcaagacta tttcttgaat tgcgtgataa aaaaggactt gcctatacgg taagaagttc
    19381 ttacgagcct aaccaaaaat gtgccaattt cagcatttac ataggaacag agcctaaaaa
    19441 tattcaaact tcaatcgaag gctttgaaca agaaattaac aaattaaaac aaattccggt
    19501 gggtgaagaa gagcttgaaa acgctaaaaa taacctgata ggaaaacagc agttcataac
    19561 agaaaccaat gcccaacaag caaatcagct ttcttattac gggattatgg atttaggatt
    19621 tgactatcag gaaaaaataa ttcaaaaaat taaagatgta acttcagccc aaattcaaga
    19681 gtgtgccaat aaatatttca gcgataaatt tatcgtttct attttgcgcc cgtaggattt
    19741 tgttcaatta taaactcatc acctttatct ataagcactt tttcaaaagc ttttttggct
    19801 ttttgttcaa aaacatcttt ccgcagataa cttgtaaatt tagttgaaac tttccaggta
    19861 tcggattttc cgtcataaga taaattggca cagtcccttt cggaaagccc ttgaagcttg
    19921 gaataaggaa tatttaattt ttgaacaaaa ttttcaagaa aattgaaaaa caagggtgaa
    19981 ttttcataac cttttgatgt ttttatgtct ttgcttccca ttaaaagtgc tctttcaaca
    20041 gcgttgtcgc cgattttttg tattaactct ttttgaatat tatcaagttt aagattttca
    20101 agctcattgg ggtcaagatg aaacatcaaa attttacgct gaccgttttg atttatgaca
    20161 aacccgccgg ctgtgcaggt tctcacggat tcagtataag ccgaaggagc aaaaacacat
    20221 ttttttaaag tacagggata gcctgcttcc aagtttttgt acattttttt attttcttta
    20281 atatggtctt cacagcgtgt aaagattatt cttgatgtaa aacttacttt tgaattttgc
    20341 ataatccccc tttttgaagc ttaaaacaat aatctccaag aagatattta tgttgtatcc
    20401 cgtaaggatc aaccctaaaa taaggctctt tattaagttc ttctgccgat ttggcaccaa
    20461 aaagagtggg cttaaagcca acttttttaa gtaaattttt aacattagca tcataaatat
    20521 ctttgtcccc tactattaat gttgatccgt cggggagttt ttcggcaaga agttttgtaa
    20581 gattttgagc accatcaggg gatagatatt gccatgaatt tctaaataaa acaatgcaag
    20641 gtttttttga taactcatta tcttttaaat ccgcaaatat atctcctgtt tgaaaattta
    20701 tgttatcaga taatttttca gttaatttaa aagtttttcc gtatcctgca tatctgctgt
    20761 ttggctctat ggttaaatag tcttccaaat ctatattttt aaaaatattt gttaatttta
    20821 gcatttcatt tttgtcaatt tttatagaat gtttttttgc tgtttctata atatcttctc
    20881 ttaaatcaat cgcttttatc ggtaaatatt tttgagcttc ttcttgaggg aagtaattaa
    20941 ataagctcat aaccaaagag caaggctcat gtcctacaga gcaggcataa tcatatatta
    21001 cagcaccgtc aggatagtat aattccaagt acttaggcaa catctcaaaa acaacgggat
    21061 ttcgaactaa ttcggtataa cttttaatat cattggtata atttgaaaaa tctttttcag
    21121 ggtttttatg cttaggataa agtgtatcat tcggatacca gccaaatgta aagttttttg
    21181 caaaatcagc ttttttataa atgggggaat aagattggat tttcatattt ttcccttaaa
    21241 ttttccgaac aaagctttaa agttcaaaaa tttaagaatt tgctatgatt tcattttgtt
    21301 gtaacatttc taaaacattt ttaagctcaa aatagtagcc cgcctcaagc agttcaaaaa
    21361 atctttgtgt tgtatttggc gcgagtgcgc gcactcgcgc cccgtcaata tataatcctt
    21421 ccacaaacct tgcaaaaagt tctgacggtt ttgaatagta tttgttatag taatttattc
    21481 tgcgcgagtt tcttgcctgc tttttttgaa gcgatttcat ccttatgtaa gcgcaaaaat
    21541 gtgcgggcat atcaggaaaa tcttgttcca atttgtcaac agaaaaaatc tccactcgcc
    21601 ttaaaaaagg cgatacaaac tttaccctgt cgtatcgaag caaatatttt gcttttgatt
    21661 ttttgatgta tttgtcgaaa tctttaaact tttttgaacg cataaaatca ggataagctg
    21721 tttttacaat attttcctga actttgattt tggctttaat ttgttccctg tgttcataga
    21781 gtttgtgtaa atgtgaattt tcgtcaacaa aacgggttac atgcaaaagc tcattttgca
    21841 aggctgaaca ggtgaatggg tgaacaggtg aaaggttaaa taatatattt acccccccgc
    21901 ctgtttttgg catattcggt tcgatttttg cgtggatata gtgagcaaat tcgtgcagaa
    21961 gtgtagggat aattctttca tctttaacat ttttagaaat gtcaatgcgg ttttgtctga
    22021 aaaaaccctg atgcccgcgt gcttttgtgg tggttttaac ctcaatccca agcgatttaa
    22081 aatattttat taattctctt ttcataattt ttctgtcatc acagtgaata aatgatttga
    22141 tttatcacta tttactaccc atcttctcgt cgttgcgagg cttctttgaa gccgtggcaa
    22201 tctttcaaac tattttatca tacaaatcaa tccagtcagg attaaatttt tttattaatt
    22261 ccaacttttt attccttgaa cctgctttga tttgtttttc tctaattata gcgttttcta
    22321 tattatcaaa acattcaaaa taaaccaatt ttgtaacgtt ataatttttt gtaaaccctt
    22381 ctcttaattt atttttatgt tcataaactc ttttgattaa atcacttgta atgccagtgt
    22441 aaagaacggt ttcattataa tttgtcatta tataaacata agattgcttc gtcatttcat
    22501 tcctcgcaac gacgtgaaag gatagacaaa aaggtcaaaa tctcatttac cccctcgcaa
    22561 cggcgttttg gcgatttaaa atattttatt aattcttttt tcatatttcc tcaaacagaa
    22621 ttttaccatg aagggagagg ctttttgtaa aaatatgtaa atatcatagc ccacatgggc
    22681 aatttatttt ttcagcacac tttaattgta tggcagcaaa aggagaaaaa atgaaaatca
    22741 ttcctataac aactttccaa atgtttaatt cgacaaattt accatgtaaa aaaccttcct
    22801 ttaaaggggt tatctctgat gttgattggg caaaccaaga aagtaaaaaa gagcaagacg
    22861 ctttttacag gacagaagtc tatgccgagc acaggaatga aatggaagga ccttttattt
    22921 gccatgacgg aacaagctat aattcaaaat tggatgcaga aattcaaaat gtaagaaaaa
    22981 gactgaataa attaaatatt tccgaagatg attttgagca attatttaaa acatttatgg
    23041 aagaaagaaa aattaatgaa gaaatagagc caaatccggt caaatattac cgtacaaatg
    23101 gtttaaaacc tatcgtagct tttttggaat tcttagaaaa aataaaagaa atgactgcta
    23161 aaaattaaag tttattttgt ttcgtgctct atccatttta agtaagtttc atctgcttcg
    23221 acaatcggaa tggctataat ttcaggtaca ttataagagt gcatgtcctg aattcttatc
    23281 ctcactcttt caaaaagttc ttttctggtt ttaataatca ttaaagcttc gtgttcttca
    23341 caaatttcac cgtcccatga aaaaacggat tccatttttt caacgatatt tacgcatgct
    23401 gcaagttttt tctctacaag tgcttttgca atttctttgc cttctttttt gttgggtaca
    23461 gtacagtaga ttactataaa ttccattttt cacctcacta aaaaatttta ttcgggttta
    23521 aaatattttt ttcatcaaaa acatgcttaa ttttacccat atattctaat gttacttcat
    23581 caatggcatt tgtcatgtac tttgattttt cacagcctat gccgtgttcg cctgaaagtg
    23641 ttccgcctaa atcaatagct aattgatgaa tttcatcctt tgttttggca taatttttaa
    23701 catcattttc atctgtcaga tcaagagaaa tatttggatg aacatttccg tcgccgatgt
    23761 gtcctacaat gcttacggta agattgtttt gggtgcaaat tcttcttatg ccttcgacca
    23821 tttgcggaat tttttctctg gggacaacaa tgtcttctgc cactacattg ggtttaagct
    23881 ttgcgcaagc cccgaatgcc gagcggcggg cggtccagat tttttgagct tcttgttcgt
    23941 ttgaagaaat tctaatatct gatgccccga attgtttgca aaattgcgct attttttcct
    24001 gctgtgaatc aagcgaaatt ataggcccat caacttccag aaacaaaacc gcgtctttat
    24061 cagtcaaaag cctcgaagga taaaattttt ctatcgtttg aattgtgttt ttatccataa
    24121 aatcaagcgt cgcaggagaa agtttatttt taagaatttg agtaactgca tttgttgcat
    24181 cagaaatttt atcaaagtaa gccaagataa cccttgagcc ttccggtttt ggaattaatt
    24241 taaaagttgc ctcgactatt atgcccaaag taccttcgga gcctataaaa aattgactca
    24301 tgtgatatcc ggtcgagttt tttgcagtat ttgagcctgt tttcaaaatc ctgccgtctg
    24361 caagaacaac ctttaaatca atcgcataat cttttgtaga gccgtattta aaggttctgg
    24421 gacctcccga gcaaagagca atgcttcctc ctatggtaga aaccgacaaa tttgaagggt
    24481 caggcggata aaaaagtccg attctttcaa cttctgcctg caaatccccc acaactactc
    24541 ccggttgaac ccttgctgtt aaattttgtt cattaatttc aagaatttta ttcattttgg
    24601 aaaaattaag cacaattccg ccgcaagtcg ttatacacgc ccctaccaaa tttgtacccg
    24661 ccccgcgcgc tactatagga atttcgtttt cataaccaag tctgacaatt ttttgcactt
    24721 gctcaatcgt ttcaacaaat accacgacac ctggaagcgt ttcaatattt tgcttgtttg
    24781 tagcatcaag tgcataaacg tatcgctctt ctattgtttc taaaacattt tcgaaagata
    24841 caatttgctt tattttatca attatcgctg catcaaattt catactaaaa ctttatcaaa
    24901 aaaacaaccc tttataaaga atttttgttt gctaaagata aagttaactc attatttttt
    24961 ttatttaaac aagaaaccaa aaaatcaact atttttttct ctaaacttat tgaaaaataa
    25021 ttattaatct cttttatgaa ataacaagga cttgttacat taatttcgat tattttttca
    25081 tctattacat caagtcccgc cataaaaacc cccatttcat taagtttttt ggcaactttt
    25141 tgaaatttta ttttttcgcc ttcggtcaat gtcgcttttt taatatattc atcgctatgt
    25201 gtgttgaact taaaatcatt gttgcccgga agcttaacca cacattcatc caaaacctct
    25261 ttgccaagtg ttaaaattct tttgtctccg taatttgcat ttgatatgta ttcttgcacc
    25321 attgttaaag cggtttggtt attggtcatg gaatttatta ttgaattaat atttttgtca
    25381 ccttttttaa gatacataac gcctacgcca aaacatttgt ttagaggttt taaaattatt
    25441 tggtcatgtt tttccaaaaa ttcaagaata tcttctttgt ttgaagttac caggttttcg
    25501 ggcatgaatt caaaaaacgt atttgcatgc agtttctcgt taaaatctct tatcgctttt
    25561 gtatcgttta ttattaaagt tttttgccta tcaacaaaat caaatatgta ggtggaattt
    25621 atataatcca aatcaacagg cggatcgggg cgaaacatca caagtgtaaa atcttgtatt
    25681 tgttgctttt gccaaatttt atcaagaaaa atattgtttt cttttaaaat aactttagaa
    25741 catttgcaaa aagcctttga attatcaaga caaagtctgt cggctgtggt tacggcgact
    25801 tcaaaatttc tttcaagaaa ctcttttatc agccaaaaat cggttataag tttattaaat
    25861 tcaaagtatt ttacttcaat tttgtcgatg acaaataaaa ttttattgtc cataacacct
    25921 ctgaaatcag catagcactt aaataaacag atacaatata ctttataact gtttttagct
    25981 taaacatatt tgtaacagaa tgtaacgaaa tttagtgatt tttgcaattt tatatataaa
    26041 aaatacttta ttaatatata acaacgacat acacagtaat ttacataact ttcaaaacct
    26101 actacacact aacctaccac acactaacct tctacacgag gaattatgaa acaagaattc
    26161 caaacccatt ttttaattaa aatgggtctt tttttgactt ctttttctaa aaataaaacc
    26221 cgcggataaa tccgcgggtt tgcaataatt aaatattgca atttgtctag tcattatatt
    26281 tttgcacacc ggggaatttg tcttgtccgg tggttttttt ggtaatccag tactggattt
    26341 ttggaatagc agttgaaaga aaatatcctg aaattacaaa accgataccc aaattaaagg
    26401 catttttcca gaaattttgt ttagccattt taaccaagaa atccgcatca ataaattctt
    26461 tttgagatga ttttgctttt ttataaagaa tttcaatata ttcagataca ttttgtttaa
    26521 gctttaacaa atcctcctgc gaaataaatg ttgtatcaca aaggtgttta cttatttctt
    26581 ctggtgcttc tttattaaat atttttgcaa aaatgctttt accttttttg taataacttg
    26641 tataaatctt atccaaaaat tccggctcat caataagtcc ttttttgaaa atatcttcaa
    26701 tttgtgattt ggacaatact gcgccttcgg gcaattcagg ctgaagtttt gccatttttt
    26761 tggctgtttc gattattgaa tcagaatttt ccaatttcaa ttctttaact ttgttaataa
    26821 attcatcaag cattatagat ttgtaatttt tatcatcggc aaacatttca ggaacaagtt
    26881 tttcatcgcc caaaagtttt actttaaatt cttcaaggct tatgccttcg gggttcattt
    26941 tagatataat atgttcattg aacacatcaa cgcttacagg atcaattctg ccttttttca
    27001 gttccttaaa caaagcttta aaggaatgaa ttccttcctt gttcccatct tgtaaaaaat
    27061 tcagcacaga gtttatatgt cctctgcaga acatgtagaa ataaatggaa gaaaggtccc
    27121 taaaaagaca ttctattctt tcatgtttgt ttcttgcgct tatgccgcgc ccaccggcaa
    27181 caccgatatc tgttgcaagg agtttataag ttgagttgtt ttctatttta taactgaaag
    27241 atagaagatt ccttaatgtc accccaccct taaaagcagg attatttgat ttttttgttt
    27301 tttcaataaa atcattaaaa gaaacatatt catgcaataa agcctgatct tctttatttt
    27361 taccgctcaa atattttttt gtaatttttt gatttatttt aggtacaaca aaacctacaa
    27421 ccaaatttgc aagaactacg cttgccataa ctttgccaaa agcaaatttt gacaaagctt
    27481 tttcaaattt atcgctgtct ttaactacat ttccggctgt ttttttcatt ttcattataa
    27541 aattgcccat aggctttctg atagcgtctt cacctgtatc aaaaccatat tcgggaagtt
    27601 taaaaacaga ttttcctaat ttatcgccta aacttctgaa aaaatctatt ccgcaaaacc
    27661 aaaatattgc gccaagagtt tcttcggtga atctttccct tgcctcgaca aaaccgtctc
    27721 tcttataggc atgaaaggtt ctgccgccgg ttacggcagc ttccagcaaa attatcggca
    27781 aaagtgcatc ggctttgccc atgtctctga caaaaacttt tgcatttttt gctactgatt
    27841 gagaaatttt gttaaattta atcattttcg gctattttta ttgactagac tactatttta
    27901 atacacctga aaaatgtttt ttgccctttt ttgaaatatt ttaacagaac tgcaacactt
    27961 tgttacaaaa aagggggatg aagagtgagc tgtgaaaagt gaacagaagc attaaactct
    28021 cacgtcattg cgaggggata aatgtatttt tgactttttg tctgcccgct cacgtcattg
    28081 caaggggtgg gttatcgact ttttcgatta ttatccaccg aagcaatcca aaaaaaccaa
    28141 ttttgtcggt cttttttaat tgggcgttga gaggctgtct tactcgttcg cgttaaacgg
    28201 gtctgcggtt tttgttttcg cggactttgt ccgctccaaa caaaagacct tcgccctctg
    28261 ctcactcgcg ctcgaactaa gaccaagctc cgcttggtag ttctcgcctt ctaaaaatcc
    28321 ccaaataaaa accctctcat agagagggtt tttatttggg cgttgagagg ctcgaactcc
    28381 cgacatcttc cttgtaaggg aagcgctc
//
LOCUS       DAOQAU010000017        27715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1705_length_27715_cov_7.227477, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000017 DAOQAU010000000
VERSION     DAOQAU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 27715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27715
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1705_length_27715_cov_7.227477"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKI94_04035"
     CDS             complement(<1..393)
                     /locus_tag="PKI94_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25942.1"
                     /translation="MTKNNLFEFDKNFNEGFVIGTDEAGRGPGAGGVFASAVYFPNPD
                     DELIKQLSDLNDSKKLSKKKREELYEIILQNSINLTVCVEVEEIEKINILNASLKAMK
                     LAVENVMKQICSGQFTAHSSQFTVLVDGN"
     gene            complement(418..1284)
                     /gene="ylqF"
                     /locus_tag="PKI94_04040"
     CDS             complement(418..1284)
                     /gene="ylqF"
                     /locus_tag="PKI94_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNW25943.1"
                     /translation="MSNETEKSQVIHGHIHWYPGHIAKAEKKLKEQLSLVDAVIEVLD
                     ARLPLSSRYGDIEKLLGEKPRLILLNKSDLVNEQELKDWTKRLEKETFCPVLTSDAKH
                     SNDLNKITNKIIELSEPRIQALMKKGLLRRAARVMVVGMPNVGKSSVINKLTKTSKAK
                     VGAKAGVTRQQQWVRINPKIDLLDTPGIIPMKQDNQDRARKLAFVNSVSQNAYEDEMV
                     ARELLLILKEKYAQNVYDYYKLKSDEDLTLENIAKSRNWIIKGAQLDIQRTSAYVLGD
                     FRNGKTGKFILD"
     gene            complement(1350..1700)
                     /gene="rplS"
                     /locus_tag="PKI94_04045"
     CDS             complement(1350..1700)
                     /gene="rplS"
                     /locus_tag="PKI94_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNW25944.1"
                     /translation="MNPVINELEKQYLDKELPELNPGDTVKVFVRIIEGNKERTQAFE
                     GTIIKKRGSGVNKTITVRKIFQGVGVERVFAIYSPRVEKINILKRGDVRRSKLYYLRN
                     LSGKATRIKEKIGG"
     gene            complement(1984..3465)
                     /locus_tag="PKI94_04050"
     CDS             complement(1984..3465)
                     /locus_tag="PKI94_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNW25945.1"
                     /translation="MFKNSRMQYVIKSVPSDNTKALENLLNEMSQGGWDLYSMHEVEA
                     EEGGYQYNCIFAGENPQEQEIAEEDVVNIKNFKTQMEKMLSSQLSPYESCRELQEKIK
                     EQRKKINKIKTQLEAQSEAPISKNRKQLNDEISKGLRDLEDLRQQLIKTISPDVMYSK
                     IKQEKLSISLSEEILDLVNPDLAAELVSETVNLRQKITDRLGYVIPKIIFQDDDNLSP
                     YEFSIKIRGQEVLNSFAYPKYLMFFEDDLKLSKKEKGAIYSVDEITGKKIVWLEEKKT
                     KSFWQKGLKPSEFICRLLENIIIKYIDEIFDYSDINRYMEIVSQENMFLIENIIPDFI
                     SAADLRYILVNLLREEVCIKDIIYIFEKINDFSDEETKEDLLDKIRLSLSRFISKGVA
                     SADNVIQAFELSEATYKSIFSKINSKETVIKIDGTKIEKIAKNIYKKAKSLNIDLENM
                     VVLTPLEARHMMFMILSQFVNNIKVLAKEEISNDFVVEIIDEI"
     gene            3614..4123
                     /locus_tag="PKI94_04055"
     CDS             3614..4123
                     /locus_tag="PKI94_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25946.1"
                     /translation="MKISAVNQHNRVNFSGKYYSREASDLLCSKAAEWWGQKINILTA
                     LDQKPASENQIKIFTQNLKKDLEKRLKEMGKINYADNCRLCGIDWHIINPFKKALHKA
                     GLKEDNPNPLKMYMDLSQGKLEVRNGCQAPQETIYELPPELNKLYDTPPENNSKSLLQ
                     KFLGLFKFI"
     gene            complement(4116..5420)
                     /locus_tag="PKI94_04060"
     CDS             complement(4116..5420)
                     /locus_tag="PKI94_04060"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNW25947.1"
                     /translation="MRIVDLIEKKKKGLKHTQEEINFIISSMMDGSAPDYQISAWLMA
                     ICFQGMNEDETTWLTDAIVKSGETVDLKELAKDVVDKHSTGGVGDKVTLTLIPLLAAC
                     EIPVAKLSGKGLGHTGGTIDKLESIPGFNTNLTIENLIKQVKKIKVAIGGQTQNLTPA
                     DGKIYALRDVTATINSMPLVASSIVSKKVASGADNIILDVKYGCGSFNKTAQDAVELS
                     ELMVKVGKRLSKSLIAVITSMEEPLGRAIGNSIEVIESIEFLKGHTQGDLTDLTYEFG
                     AIALIQVGKFDDEKKAKEYLQNVVKSGKALEKFRELIKAQGGNPEVVGDYSKFPQAKY
                     KVEIKSGKEGFISKIDAYQIAYGCKLLGAGRERKTDDIDYSVGVYLNKKHGEFCKKGE
                     VLYTIYSNDEAKTKLAQDVCDQAFELTSKTPKCQDLIYKIIK"
     gene            complement(5550..5996)
                     /locus_tag="PKI94_04065"
     CDS             complement(5550..5996)
                     /locus_tag="PKI94_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNW25948.1"
                     /translation="MKIKFTVLAFAIMVFAWCSLPAKAEGGIGYINYKKIQESCPFAQ
                     AAVKEVDAKGLELQQYLVDKEKEFKALDTPIKKKNFEEKTAKEFKLKEEAYMKLKMQK
                     EEAVYNKIQEAARQVLIEQKLDTIVDFRIIFVGGIDVTDLVLQKLK"
     gene            complement(6084..8330)
                     /locus_tag="PKI94_04070"
     CDS             complement(6084..8330)
                     /locus_tag="PKI94_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNW25949.1"
                     /translation="MRVLGSIYKALKIFLVFVILACAMPVFAQKSKSDQESLQMPEDA
                     KLMQQIFSVSSEDASNVFAPDAQRVPLPDVSVKKRTWFKKPAEDVKQADVSPPEVSPV
                     TIPVETPVIAAPSVPSTVPAAQPVLPPVSVPMPSSAASKAVPETPISSVNSSTNIAKE
                     GEKEAVYIRDIEIAGVNVIKPEMIMNKLQFRTGDEYSRDMVQNNLKAIYQMGFFTERM
                     KAVPVENPDGTVTLKIYLEENTPVSDFTVEGNEVVSTEEILNFLTPLQGEPQNIADIN
                     TAIEKIQEYYSSKGYILARIDSLYDDPDGVINVHLNEGEIKRILIAGNEKTKDYIVAR
                     NILTEAGMVYNENLIREDLVRLYATQAYKDVKREIEPSADDPEKYDVTIQVEEQRTAS
                     VMIGGGLDSATGLFGSGGIADNNFRGLNQRVALNLLAGSGFILNDSSIINHMNMQAEL
                     SFFEPYFMNSDNSLMNKIFFRDFGSYQIPLAIERRIGAEATIAHKVKNNKNLTGTLSL
                     GLENVNVREGDETAIAQLYAQHSVPIANRAQQLEGGTFLSLSPGLIYDTRDTVMNPRH
                     GLMATVRWDESLDVGGFDKTFGKVTGSVKKYVPIGKKSSLSFLAKAGGKIHGDMPEVM
                     AYRLGGPYSIRGFRMSGVGTGDGFVMGSVELATPIPLLDRLKVQFLDNVRMTFFVDAG
                     KIFNSTISDVIYDRPMQAVTAGVGLKIYIPGMGPLSVDYGVPILNPGNTGSTRGYFTF
                     GVGDMMMY"
     gene            8505..9740
                     /gene="tyrS"
                     /locus_tag="PKI94_04075"
     CDS             8505..9740
                     /gene="tyrS"
                     /locus_tag="PKI94_04075"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNW25950.1"
                     /translation="MDSTKTVKEEFLQQAKFLARSAEVLPGGVEEGIKELAIKLQKAK
                     ETNTPLRIKLGMDPTRPDLHLGHCVVMRKLREFQKLGHKIVLIVGGATAMVGDPSGKS
                     ETRPSLTKEEVEKNAKSYFEQMSKVVDIKNAQIVNNADWLHKMNFVDVLKLSSHITVA
                     QMLVREDFAKRISENKPISVHEFFYPLMQAYDSVIVDSDIEIGGTDQRFNVLLGRDIQ
                     AAYGKKYPQMVMLLPLLEGTDGVVKMSKTYPEHCISINENPKDMFGKLMSIPDTLIIR
                     YYTLLTDVSDEDLKEIENQLKSGVNPRDIKLKLAHTITQEYCGTEGAEFGKNEFINVV
                     SNKGIPTDVPEYKLKGETNILDLLVELNFVTSKGKAKRLIQGGGVKFNSEKISDMNYQ
                     INKEGILQAGKRNFVKLIK"
     gene            9748..10665
                     /gene="cysK"
                     /locus_tag="PKI94_04080"
     CDS             9748..10665
                     /gene="cysK"
                     /locus_tag="PKI94_04080"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNW25951.1"
                     /translation="MIHNNILELIGKTPLVKLRTFNTKGCEIALKLEFFNPSGSVKDR
                     ASFYMLNEAIKAGKINKNTVIIEPTSGNTGIGLAMCAAVMGLKIVIVMPESMSIERRL
                     LIKGYGAKLVLTPKKKGMQGAVDKAFELAEKDPNSFIPSQFSNPANTLAHKETTSKEI
                     FEDTKGKVDIVVAGIGTSGTTMGLCEGLKALKPSVKIYGVEPMESPLLTEGRSAPHKI
                     QGIGANFIPKLFDKKKIDGIITVNAQDSFKFAKELAKREGILVGISSGAILKAALDLA
                     KKSENKGKLIVAIMCDFGERYLSTELFES"
     gene            10791..11441
                     /gene="cysE"
                     /locus_tag="PKI94_04085"
     CDS             10791..11441
                     /gene="cysE"
                     /locus_tag="PKI94_04085"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNW25952.1"
                     /translation="MLTRTIQTIKEDIKTIYKKDPAAKNLLEVIFCYPGFHALLIHRF
                     SHKLWVWKIPVIPRMLSTFARFLTGIDIHPGATIGRRFFIDHGMGVVIGETTIIGDDV
                     LLYQGVTLGGTSSKPEKRHPTLCNNVVVGAGAKVLGNITIGEYSKIGAGSVVIDNVPE
                     HATVVGVPGHIVMQKIIDENGVLMHNRIPDPISCEINRLKYEVKELQEIVKGEKNN"
     gene            complement(11506..12879)
                     /locus_tag="PKI94_04090"
     CDS             complement(11506..12879)
                     /locus_tag="PKI94_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNW25953.1"
                     /translation="MDKRELESGASLFGRSMNLIEEDPLEEIFALNLGDFISEHLISE
                     DLVQKIGHNVKDKAESLKSQLKELISIYSIDNTLCVLGFSSKEDYVIYNSIAKTIVQM
                     LDMDVCHIYLTKENAKGLGKSSKDLLLVGSSIEIKDDLYKYDLGYKFDDKSFVSEAFK
                     NKETVEVKNPNFSKRFEPIKKLNEDKVKVFTAIPMHNNAKAVGVIVIQSYVDRFFESE
                     YLRLIESTASLFATSMELQRVIEEANELIASEDSIIADLQHMRAELTALIGDLGDQQQ
                     TFVKRLASAVDTKGQYKVSHSQNTADLSRLICKHLGLNEKTTDLIYYAGLLQNIGKIT
                     LPETLFSHTGKLDKEDWEKLQNHPNVGVNLLMNINFLSEVVPYIHYHRERWDGMGEPE
                     GLRGNSIPLGSRIIAVADAFSAMTADRPYRKAMLKNQALEIMKNEAESKWDPIIVDAL
                     AQVIKQM"
     gene            13244..13432
                     /locus_tag="PKI94_04095"
     CDS             13244..13432
                     /locus_tag="PKI94_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNW25954.1"
                     /translation="MARVLISMPEKFLDEIDSVAGNENRSRSELIREALRTYIHRNKV
                     RENVTATKNATILEALLD"
     gene            complement(13594..14484)
                     /locus_tag="PKI94_04100"
     CDS             complement(13594..14484)
                     /locus_tag="PKI94_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25955.1"
                     /translation="MTASIGTFAPYYPQSGICNNGNLSGDDIFYGNDDFLSSSIFNNN
                     GFNTLNNLGGGCFGPTILQNSYGYPGQMPMMQEGETLEEYAKRVKDYVGLQGEIQNDM
                     QKSQLNNQVEYQKRMNGVNFDLTSEEDAITRQIAILQSKIKANEQDQIMSCYNKLLQS
                     AKQQLAAQGIDVSKISNDQLKAYVEKLYNQATGKSMTDEIAENGTGQFWSGFGKGFFG
                     LGTLFGNSRTAQDNISDITGEPLSKSQMTSKAVGGSLGVVAAGAICVGAGVLCPPLGI
                     ALGVALGAGIISLFSKKKKA"
     gene            complement(14654..15256)
                     /gene="clpP"
                     /locus_tag="PKI94_04105"
     CDS             complement(14654..15256)
                     /gene="clpP"
                     /locus_tag="PKI94_04105"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNW25956.1"
                     /translation="MDNLQVLNYVPTVIEASSRGEKAFDIFSRLLRERIIFVGDEIDD
                     ESANSIVAQLLLLDSENPEKDIMMYINSPGGVITAGMAIYDTMKLVKADVSTICIGEA
                     ASMGAFLLSAGTKGKRMALPSARIMIHQPLGGAKGQATDIEIEAKEIIRMKNMLNGLL
                     AQHSGQTIEKIKEDTERDYYMSAQEAVDYGLIDKVLSASN"
     gene            15348..15551
                     /locus_tag="PKI94_04110"
     CDS             15348..15551
                     /locus_tag="PKI94_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25957.1"
                     /translation="MYCIDDIVYIEDDDQCISCENYARGVACPLIEALGMGVVYLEDS
                     LTVTNCGFYKEFKRNLRIVKNEQ"
     gene            15548..16540
                     /locus_tag="PKI94_04115"
     CDS             15548..16540
                     /locus_tag="PKI94_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNW25958.1"
                     /translation="MRTVTLIPGDGIGPEITDCVVKIIKKSGLELNWDVQTAGKDVIK
                     KEGTPLPQRVLESIKKNKIALKAPVTTPIGEGFRSVNVTLRKELDLYANLRPCKNIKG
                     IKTKFDDVDIIVVRENTEDLYAGIEKQIDKNTAQSIKIITREASERIAEFAFEYALKN
                     SRKEVCSVSKANICKLSDGLFLESVRDVAKKYPQINYKEILVDNLCMQLVQNPQKFDV
                     LVLPNLYGDIVSDLCAGLIGGLGVAQGANIGFHHAVFEPVHGSAPDIAGQNKANPTAL
                     LLCAIEMLKYMGENFYAQKIEQALLKTLQGKILTPDLGGSATCTKFTEEIIKLL"
     gene            16672..17430
                     /locus_tag="PKI94_04120"
     CDS             16672..17430
                     /locus_tag="PKI94_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW25959.1"
                     /translation="MRENFRGFTLAEVLITLTIIGVVAALTVPNLYAYYQKTQYVTQL
                     KKIYTEISQALKLMMADESINKITASDTLYCDETAGCIWDEAYQRAGNDFLKKYFKVI
                     KDCGRENLSTCFADNYKSISGNEAGGIGCYYSVIIASGASICISGQSGTYNAGTFIVD
                     INGLKLPNVAGRDLFTFSYYYDGSLDESITPECRKGIANASGLCSNYGSNAQEAREEY
                     FNNGRGGQPGCLGNSYSAIGCFGKILNDNWKMDY"
     gene            complement(17612..18262)
                     /locus_tag="PKI94_04125"
     CDS             complement(17612..18262)
                     /locus_tag="PKI94_04125"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNW25960.1"
                     /translation="MKTVAFIPARGGSKSIKNKNIMPLAGKPLIYWAISACIGSKSIE
                     KVFVSTDSNEIKKVVEGFGFKNVEVISRSPETATDTATSESALIEFCKNHKFEKVVFL
                     QATSPLTASADLEGALKKMKKEGSDSLVSAIRNYQFLWDIKGKPINYNPQKRPRRQDW
                     DGYMIENGAFYISSRENILKSKCRISGKITFWEMDSKSFFEIDNPEDLAIVEKFIR"
     gene            complement(18259..18810)
                     /gene="hpt"
                     /locus_tag="PKI94_04130"
     CDS             complement(18259..18810)
                     /gene="hpt"
                     /locus_tag="PKI94_04130"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNW25961.1"
                     /translation="MQVKDMEIKKEDLKILFTAEQIQHRIKEIALHLNSIYKDEEVYV
                     VCVLKGAVMFAVDLVKYLKMPLKMEFIRLSSYGHSTTTSGKVNAVDISLPDLNGKNVL
                     VVEDIVDTGLTAKFLLDFINNNFKTKTTKFCSLLDKKCFRKTQIEPDFVGFDVDDKFV
                     IGYGLDFEGHFRNIPYIGHVENL"
     gene            complement(18820..20181)
                     /gene="tilS"
                     /locus_tag="PKI94_04135"
     CDS             complement(18820..20181)
                     /gene="tilS"
                     /locus_tag="PKI94_04135"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNW25962.1"
                     /translation="MIEIVENFLKKYDLISSENTFLVGFSGGCDSMALLDILKTLSKK
                     HRFKVVAAHLNHNWRGEESLQEEKNCEKFCKKNKIEYVCQTLENTVDKSENTAREARY
                     KFFVSLAKKYKNCSIFTAHSASDNAETLIYRIIKGTGIKGLQGILPERKIDGHSVFRP
                     LLGLAREEIEGYCISRGLVANTDSSNFDTNYKRNFIRHKIMPLFKEINYSAPMAIVSL
                     AKIASNQNKIVEEYLNLIKSEIYCEGKILTPKFKNLSKEVRQKIIYDRVLEHNLDYDF
                     KKIKSISDFIEENWNSKAGSRYSVTSDLWLFINKENMYLIDKLQNKKNISIKINKEGE
                     YKIPEIEGAFSLQKYKGAEKIEFPCENALCAYVEIKVKIDFELRTRKEGDFINPFGMK
                     GTMKLKKYLNSKGVAQHEKDSLLMLCSGSEVFWVIGVGLSDKFKVVNKPSHVLEFRGK
                     CRG"
     gene            complement(20280..21638)
                     /locus_tag="PKI94_04140"
     CDS             complement(20280..21638)
                     /locus_tag="PKI94_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNW25963.1"
                     /translation="MFEKFTEKAINAVTEAQNQASLMDNALVQPEHLLLAIAKEAKGV
                     SQKIFKAQNINFEELKEAVGKRLRFEKAESPQEARPFSNEFKQLLKRTLDLANKSGNS
                     FVLFEHLFIAVLNDKTSYIRRILEQFNFNIYESREILYKLVEKKSKKLSHPEIEDTKE
                     FESSYLTADCLFEYEESSKIFERAVSKLSASNYEILGTEQIISSILEDKESDIVKILN
                     NEGITLEAFENELSKQSSRASEFEGKKVIFTPNAFVAMNLAIQAAKELGSLEIKPEHM
                     ILGLLKAKKGVAYDIFKALNVDENSLGEEIVKPIEKQMPQALTILKLAKQEARRLGRN
                     IVGTEMILLGILNEGMGVGFKVLNELEINIKDARQVIEGLIGYGNEYYETEIIFTKRA
                     KKVLEIAWEKAKKHCKSRILSEHLLYAITMEPQSVAMKALEQLGVDAVEIKQGILKEI
                     ER"
     gene            complement(21638..21907)
                     /locus_tag="PKI94_04145"
     CDS             complement(21638..21907)
                     /locus_tag="PKI94_04145"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNW25964.1"
                     /translation="MKEKTKEENFIIETIDEEGKIIKFELFDIVEFEDKEYALLLPAQ
                     NPKDDDEVVLMRLKQDGEDYLFEAIESDEEFDKVAEFIESLDVED"
     gene            complement(22041..22448)
                     /gene="ruvX"
                     /locus_tag="PKI94_04150"
     CDS             complement(22041..22448)
                     /gene="ruvX"
                     /locus_tag="PKI94_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNW25965.1"
                     /translation="MYKVMALDVGTKRIGVALSDFLLILANGNSCIERVPEKNAVEKI
                     KKIAQENKVKKIVIGVPLNMDGTKGSQAKDCINFAKNFENEFEIIFEDERLTSQQAEE
                     NLREKKINFKKDKGLVDIESACIILEQYLSRER"
     gene            complement(22441..24090)
                     /locus_tag="PKI94_04155"
     CDS             complement(22441..24090)
                     /locus_tag="PKI94_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25966.1"
                     /translation="MSNNQLNKLQIKAEVLTVLSKLQTLPEVSGAEALMEVLLIQQDK
                     RAILDVLIKELLKATEQRAVLICYMLLRLCEKEELEKPLWDILKSPQVNDGIKSVVMN
                     LLRDLGNRVNYEKLGEYFENPDDVIDADTKKLLHVAIINPEAQIDFLDFLKSLPDKDK
                     EILIKSLGEDYSQDELANILIPLFLYSPESDLGKISVGILGETKSQLALHALIEALDF
                     VEDEQTVLLIKKNISTLKIAGVRQDNTIEFYKEILSASSPFESYISYPDGHGNQAVIF
                     SRIRDIETIQMFAVVINSGWGIVDCFGFNEISKAEFERIVKRFFDGDEHTQIPPSFVK
                     YILKNAQTITRKTGGDISYEFLCWKTLLSDIQETENFEKILDENFVKRKLSDSEMEKF
                     YFAPFIQRWFLDTEYSQEFTKFVEGLNQKFIEDDFEVDLELQIQEKLSDIFCESQRKS
                     IEDRLLMCAYLEFLKKDSIDAQILYALRFDEENKVKLYENMLRKSIYEYYVLQKFKQI
                     EEKKTANIFALRNKQKEKGLSVVQIEKIISMIENLWVRENV"
     gene            complement(24087..24344)
                     /locus_tag="PKI94_04160"
     CDS             complement(24087..24344)
                     /locus_tag="PKI94_04160"
                     /inference="COORDINATES: protein motif:HMM:NF018404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HNW25967.1"
                     /translation="MSDKVVKLGSKKSKPEKKKEETQEVVICSFCNRPNTQVIKMVQG
                     PGVNICSECTMIAVQYLILNDRMPSAEAQMILDAFWGKSRK"
     gene            24488..25093
                     /locus_tag="PKI94_04165"
     CDS             24488..25093
                     /locus_tag="PKI94_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW25968.1"
                     /translation="MKKAFLLVTILFVSVISTACINNLAVQELNNKAKSYLDKGDYEN
                     AISRLKSSLDLDSSFFETHYNLGIAYTQAEKYPEAVETFESAIKIKPDFADTYYSLAV
                     AQENYAKGIIDGTIKDEDKDEDKEQEDKIDAEEETHQNKNLSAQEKTKVCELLDKAVQ
                     SYQTYLEKGLEIKDKEEVEEKIEYLKIQIEKYSSQPPLKEG"
     gene            25095..25901
                     /gene="lgt"
                     /locus_tag="PKI94_04170"
     CDS             25095..25901
                     /gene="lgt"
                     /locus_tag="PKI94_04170"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNW25969.1"
                     /translation="MFVSPGDIFFSIGAFSIYYYGIIMALSLLLGVLVASNITKKLYP
                     ELNSEIIYDISPYIIMCGIIGARLYYCLLSSEYYLAHPKEIIQIWQGGISIHGAIIGG
                     LIGGILYAKAKKLPILKLCDIFSYGLILGQALGRWGNFFNSEAFGLPTDNFFKLYIPI
                     YKRPIEYLQYNFFHPTFLYESILDIIIFFILLFVVRKLTKNIDGIIFFSYLTMYSVAR
                     ILIEQIRIDSVLNIFGIPIAQIVSLALFFVGIIGIFILRLRNESSRQTDS"
     gene            25873..27282
                     /gene="rlmD"
                     /locus_tag="PKI94_04175"
     CDS             25873..27282
                     /gene="rlmD"
                     /locus_tag="PKI94_04175"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNW25970.1"
                     /translation="MKVQDKLTLDIEKLSNLGFGIAKHEGLVIFVENTCPQDKVLAEI
                     TKLNKNWATAKIVEIIKGSAHRAEPFCAMQKVCGGCQLQFINYDYQLECKKEIIEDAI
                     KTIGGLNVEIKNPIASPQIKNYRHKVQYPISQTKVSGRILAGYYKPKSHEIVNIKHCP
                     IQPEICDKIIEFIRNTAFNFGISGYNEKKHAGDLRNVVMRVSASSGKILVTLVFNSLP
                     LTPALSRKGRGSWIKERIYPFAQNIFDNFKEVTGVCINFNSKKTNVILGETTELVVGN
                     DFIKEQILDRSFKIGPNTFFQINPKSAQNIFKFVKKYIKNNYEQPLVLDAYAGVSSFG
                     ICVSDVCRKVVSVEENKESVSLAQEILNLNDIKNVELHNMDTTKFFEKELKTKKRKFD
                     VIILDPPRKGCSEESLEYALNLCKGKIIYVSCNPATLARDLKYLTSKGAKVEFIQPFD
                     MFCHTYHIESVAIINCNIL"
     gene            27369..>27715
                     /locus_tag="PKI94_04180"
     CDS             27369..>27715
                     /locus_tag="PKI94_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25971.1"
                     /translation="MNIQSISAFNSLTSKKIAFSKSNSNVEKLPDGQVIVTDYWADGK
                     TPMKRTLYADETLKVPVRFALYRADGTERIVHDAMPMVNKNNQKPVFKINKETLPDGQ
                     VIVTDYWADGKTP"
BASE COUNT         8745 a         5394 c         4569 g         9007 t
ORIGIN      
        1 attgccgtca accaaaacag tgaattgtga actgtgagca gtgaactgac cggaacaaat
       61 ttgtttcatt acattttcta ccgcaagttt catagccttt aatgatgcat tcagtatgtt
      121 tattttttct atttcttcca cttcaacaca aacagtcaaa ttgattgagt tttgaagaat
      181 tatttcatac aattcttctc gttttttctt tgataatttt tttgaatcgt tcaaatcgga
      241 aagttgttta attaattcat catcaggatt tggaaaataa accgcacttg caaaaacccc
      301 gcccgcaccc ggccccctgc ctgcttcgtc tgtaccgata acaaagcctt cattaaaatt
      361 tttatcaaat tcaaatagat tattcttagt cacttagtca cctggtcact tattcactca
      421 atccaaaata aatttccccg tttttccgtt tctgaaatcg cccaaaacat aagccgaagt
      481 tctttgaata tcaagctgtg cgccttttat aatccaattt ctgctttttg ctatattttc
      541 aagcgttagg tcctcatctg attttaactt ataataatca taaacgtttt gcgcatattt
      601 ttccttaaga atcaagagca attcccttgc aaccatttca tcctcataag cattttgcga
      661 aacagaattt acaaaagcca gtttccttgc cctatcttga ttgtcctgct tcataggaat
      721 aattcccgga gtgtcgagca aatcgatttt agggttaatc ctcacccact gttgctgtct
      781 tgtaactccc gctttagccc cgactttggc cttagaggtt ttggtcagtt tgtttatgac
      841 actggattta cccacattag gcatcccgac caccataacc cttgcggcgc ggcgaagaag
      901 cccctttttc attaatgcct gaattctggg ttcgcttaat tctattattt tatttgttat
      961 tttgttcaaa tcatttgaat gtttggcgtc agaagtcaaa acagggcaaa aagtttcttt
     1021 ttctaacctt tttgtccaat ctttgagttc ttgttcattg accaaatcgg atttattgag
     1081 caaaattaaa cgcggttttt cacccaaaag tttttcgata tcgccgtatc tgctgctcaa
     1141 aggaagcctt gcatcaagta cttcaattac cgcatcaaca agcgaaagct gttctttaag
     1201 ctttttttcg gcctttgcaa tgtgccccgg gtaccaatga atatgaccat gaataacttg
     1261 gcttttttca gtttcattac tcatggtcta tacctctttt ttcagtgaat aagtgaataa
     1321 gtaaataggt gaaagatcac cttttacatt taccccccta ttttttcctt aatacgtgtt
     1381 gctttgcctg aaagatttct caaatagtac aattttgaac gtcttacatc gcctcttttg
     1441 agaatattta ttttttctac tctcggagag taaatcgcaa aaactctttc aacaccaacg
     1501 ccttggaaaa tttttcttac tgtaattgtt ttgttaacac cgcttccgcg ttttttgata
     1561 attgttcctt caaaagcctg cgttctttct ttgtttcctt caataattct cacgaaaact
     1621 tttactgtat caccggggtt caattcagga agttctttat ccagatattg tttttctaat
     1681 tcattaataa cagggttcat gacctcatcc tttacttttt cgactgtgta actatattaa
     1741 tactatttaa aaaataattt aaaatcaagt ggggggggta aatgagaaag ggtgaagtgt
     1801 gagcggtgag cgttgaaggg ttaaatcagc ttaaaatttt ataagtttca tatttctaag
     1861 ctactaaatt tcttattacg cctacccgtc atattgaggc tttagccgaa atatctcaga
     1921 gattcttcgc tatggctcag aatgacaaaa cttctcactg ctcacttctc actgctccct
     1981 tttttatatt tcatcaataa tttcaactac aaaatcattt gaaatttctt ctttcgcaag
     2041 cacttttata ttattgacaa actgtgacaa aatcataaac atcatatgtc ttgcctcaag
     2101 cggagttagc acaaccatat tttccaagtc aatatttagc gattttgctt ttttataaat
     2161 gttttttgca attttttcga tttttgtgcc gtcaattttt attacggttt ctttagaatt
     2221 aatttttgaa aaaatactct tgtaagtagc ctccgaaagc tcaaatgcct gaataacatt
     2281 atctgcagac gccacgccct tgcttatgaa gcgagagagc gaaagcctta ttttatcgag
     2341 caaatcttct tttgtttctt catcggaaaa atcatttatt ttttcaaata tatatataat
     2401 atcttttatg caaacttctt ctctcaaaag atttaccaga atgtatctca aatcggcagc
     2461 agagataaaa tcgggaataa tgttttcaat caaaaacata ttttcttggc ttactatttc
     2521 catatagcgg ttaatgtcag aatagtcaaa aatttcatct atatatttaa taataatatt
     2581 ttcaagcagt ctgcagataa attcggaagg tttcaatcct ttttgccaaa agctttttgt
     2641 ctttttttct tcaagccaga cgattttttt gccggtaatc tcgtcaacag aatatattgc
     2701 gcctttttct tttttgctta attttaaatc atcttcaaaa aacatgaggt attttgggta
     2761 agcaaaagag ttcaagacct cctgtcctct tatttttata ctgaattcgt aaggacttag
     2821 attatcgtcg tcttggaaaa ttattttcgg gataacatat ccgagtctgt cagttatttt
     2881 ttggcgcaaa ttaaccgttt cagataccaa ttcagctgcc aaatctgggt ttaccaaatc
     2941 caaaatttct tcgcttaaag aaattgataa tttttcctgc ttaatttttg aatacataac
     3001 atcgggcgaa atggttttta taagctgttg gcgcaaatct tccaagtccc ttaaaccttt
     3061 tgaaatctca tcgttgagct gttttctgtt ttttgaaatc ggtgcttcgg attgtgcctc
     3121 aagctgagtt ttaattttgt ttattttttt tctctgctct tttatttttt cctgaagttc
     3181 cctgcaggac tcataaggcg aaagctgcga agagagcatt ttttccattt gggttttaaa
     3241 atttttgata tttacaacat cttcttcagc gatttcctgt tcctgtggat tttctcctgc
     3301 aaaaatacag ttgtattggt atccgccttc ttctgcttca acctcatgca tactgtataa
     3361 atcccagccc ccctgagaca tttcattgag cagattttca agtgcttttg tattatcgct
     3421 tggcactgat tttataacgt attgcattct ggaattttta aacatctgtt gctccttatt
     3481 ttttgtattt taattgtacc aataataaat tttttcacaa tgttatgatt tgtaaatata
     3541 aaaaatatta ctagcaaaaa aaaatattat gaactttata atattaacat ttttataaaa
     3601 tcaaggagaa aaaatgaaaa tatcagcagt taaccaacat aatcgagtga atttttcagg
     3661 caaatattac agcagagaag ccagtgactt attatgcagc aaagctgccg aatggtgggg
     3721 gcagaaaatc aacatattaa ccgcgttaga ccaaaaaccg gcaagtgaga atcaaataaa
     3781 aatatttaca caaaacttga aaaaagattt agaaaaaagg ctgaaagaaa tgggcaaaat
     3841 aaattatgcc gataactgca gactttgcgg aattgattgg catataataa atcctttcaa
     3901 aaaagcctta cacaaagcag ggctaaaaga ggataatcct aatccgctta aaatgtatat
     3961 ggatttaagc caaggtaaac ttgaagtcag aaacggttgt caggctccac aagaaaccat
     4021 ttacgaatta cccccggaat taaacaaact ttatgacact ccgcccgaaa acaattcaaa
     4081 aagtttatta caaaaatttt tagggctatt taaatttatt taataatttt ataaattaaa
     4141 tcctgacatt ttggggtttt tgaggtaagt tcaaaggctt ggtcgcagac atcttgtgca
     4201 agtttggttt tagcctcgtc atttgaataa attgtgtaca aaacttcgcc ttttttacaa
     4261 aattcgccgt gttttttgtt taagtaaaca cctacgctgt aatcaatatc atccgttttt
     4321 ctttctcttc ctgcccccaa caatttgcat ccgtaggcaa tttggtaagc gtcaattttg
     4381 cttataaatc cttcttttcc ggatttaatt tccactttat attttgcctg agggaatttt
     4441 gaataatcgc ctacaacttc agggtttccg ccttgcgctt tgattaattc cctaaatttt
     4501 tcaagtgcct tgcctgactt aactacattt tgcaagtatt cttttgcctt tttttcatcg
     4561 tcaaattttc ccacctgaat aagcgcaatg gcaccaaatt cataagtcaa atcggttaaa
     4621 tctccctgcg tatgaccttt caaaaactca atggattcaa taacttcaat cgaattacct
     4681 atagcccttc ccaaaggctc ttccatggat gttatgactg caattagtga ttttgaaagt
     4741 cttttgccga ccttaaccat gagctctgaa agctcaaccg catcttgcgc agttttatta
     4801 aaagagccgc agccgtattt gacatcaaga attatgttat cagcaccgga tgcaactttc
     4861 tttgagacaa tagaagaagc taccaaaggc atactgttaa ttgttgccgt aacatctctg
     4921 agtgcgtaaa tttttccgtc tgcaggggta agattttggg tttgaccgcc tattgcgact
     4981 tttatttttt taacctgttt aattaagttt tcgatggtca aattcgtatt aaatccgggg
     5041 attgattcaa gcttgtcgat agtgcctccg gtgtgcccca aacctttgcc cgaaagcttt
     5101 gcaacaggaa tttcacaggc tgctaaaagc ggaattaaag tcagcgtaac cttatcgccc
     5161 acgccgccag ttgaatgttt gtccacaacg tctttggcaa gttcttttaa gtcaactgtt
     5221 tcacctgatt taacaattgc atctgtaagc catgtcgttt catcttcgtt cattccctga
     5281 aaacaaattg ccataagcca agctgaaatc tgataatccg gcgcagaacc gtccatcatg
     5341 gaagaaatta taaaattaat ctcctcttgc gtatgtttca gccccttttt ctttttttcg
     5401 attaaatcaa ctattctcat agtttaacac ccaatcactt atcacgtcat tgcgagcgac
     5461 tgcggcaacc ttttcaactc ttaaaagcgg attggattgc ttcaccctaa agggttcgca
     5521 acgacgaaaa taataacttt tctcttgtct tactttaatt tttgcaaaac caagtcggta
     5581 acatcaattc cgcctacaaa aattattctg aaatcaacta ttgtatcaag tttttgctca
     5641 ataagcactt gccttgcagc ttcttgaatt ttgttataaa ccgcttcttc tttttgcatt
     5701 tttaatttca tgtaagcttc ttctttcaat ttgaattctt ttgctgtttt ttcttcaaaa
     5761 tttttctttt taattggagt atccaaagct ttgaattctt tttccttatc cactagatac
     5821 tgctgaagtt ccaaaccttt agcatcaact tctttaaccg cagcctgtgc gaaaggacaa
     5881 ctttcctgaa tttttttgta attgatgtag ccgattccgc cttcggcttt agcaggtaac
     5941 gagcaccacg caaagaccat gattgcaaat gccagaaccg taaatttaat tttcataatg
     6001 cctccttttt ttagtgagcg gtgaaccgta aactatgaga agttctctta ttcacttatt
     6061 ctcctattca cttattcact tatctaatac atcatcatat caccaacacc gaaagtaaag
     6121 taccctcttg ttgaccctgt attacccgga ttgagaatag gtaccccgta atcaacagaa
     6181 agaggaccca tacccggtat ataaattttc aaaccgacac cggcagtaac cgcctgcata
     6241 ggtctgtcgt atataacatc agaaattgtg gagttaaata ttttacccgc atcaacaaag
     6301 aaagtcattc ttacattgtc aaggaattgt actttaagcc tgtctaacaa aggaatcggc
     6361 gttgccaatt caaccgaacc cataacaaat ccgtcacctg taccaacacc gctcattctg
     6421 aaacctctta tcgaataagg tccgcctaag cgatatgcca taacttcagg catgtccccg
     6481 tgtattttgc ctcctgcttt agccaagaat gacaaagaag actttttgcc gataggcaca
     6541 tattttttaa ccgacccggt aactttgccg aaagttttat caaaacctcc tacatcaaga
     6601 gactcgtccc accttacggt tgccatcaaa ccgtgcctgg ggttcataac cgtatctctt
     6661 gtgtcataaa tcaaacccgg actcaaagaa aggaaggttc cgccttcaag ctgttgagcc
     6721 ctgtttgcaa tcggaacact gtgctgagcg taaagttgtg caatagctgt ttcatcgcct
     6781 tctctcacat taacattttc caatcctaaa gaaagtgttc ccgttaagtt tttattgttt
     6841 tttactttat gagcaatagt tgcttcagca cctattctgc gctctatcgc aagcggaatc
     6901 tgataactgc cgaaatctct gaagaaaatt ttgttcatca gggaattatc cgaattcata
     6961 aaataaggct caaaaaagct caattctgcc tgcatgttca tatgatttat tattgaagaa
     7021 tcatttaaaa taaagcctga acctgcaaga agattaagtg ccactctttg gtttaagcct
     7081 ctgaagttgt tatctgcaat accgcctgaa ccaaaaagcc ccgtagccga atcaagacct
     7141 ccgcctatca taacagatgc ggttctttgt tcttcaacct gtattgtgac atcatatttt
     7201 tcaggatcat ccgcagacgg ctcaatttct ctctttacgt ctttataagc ttgagttgca
     7261 taaagtctta ccaaatcttc tcttatcaaa ttttcgttat agaccatgcc ggcttctgtt
     7321 aaaatgtttc ttgctacaat atagtctttt gttttctcat tccctgcgat taaaatccgc
     7381 ttaatttcac cctcattaag gtgaacattt ataaccccgt cgggatcatc gtaaagcgaa
     7441 tcgattcttg caagaatata gcctttagaa gaataatatt cctgaatttt ttctattgct
     7501 gtattaatat cggcaatgtt ttggggttcg ccttgaagcg gagtaaggaa gttaagaatt
     7561 tcttctgtgg aaacaacctc gtttccttca accgtaaaat cacttacagg agtattttct
     7621 tccaaataaa ttttcaaagt gaccgttccg tcaggatttt caacaggaac cgccttcatt
     7681 ctttcggtaa aaaatcccat ctgataaatt gctttcagat tgttttgaac catgtccctg
     7741 ctgtattcat cgcctgtacg aaactggagc ttattcatta tcatctcggg cttaattaca
     7801 tttacacccg caatttcaat atctctgata taaaccgcct ctttttcacc ttctttcgca
     7861 atatttgttg aagaattaac agaagatata ggggtttcag gaacagcttt agatgccgca
     7921 gaagacggca taggaacaga aacaggaggt aaaacaggct gcgccgcagg tacagtcgaa
     7981 ggaacagaag gtgctgcaat tacaggagtt tcaacaggaa ttgtaacagg ggaaacttca
     8041 ggaggtgaaa catctgcctg ttttacatct tcagcgggtt ttttaaacca tgtccgtttt
     8101 tttacggaaa catcaggcaa gggaactctt tgtgcatcgg gggcaaaaac atttgaagca
     8161 tcttcggatg aaacagaaaa aatttgctgc attaactttg catcttcagg catttgcaag
     8221 ctttcttggt cagatttact tttttgcgcg aaaacaggca tggcacatgc caagatgaca
     8281 aataccaaaa atatctttaa agctttataa attgagccta aaaccctcat tatcccccgt
     8341 aagtacgaac aaaaagttat tgtagttccc caaaatctgt tcctagaaaa tattatactt
     8401 caaaatcagc gcaaaattaa attgtaaaag aaaaattaat ataatgttta caaaacaatg
     8461 catttttttg gtataattga gccaaaatga aagaggctta gaaaatggat tctacaaaaa
     8521 ctgtaaaaga agaatttctg caacaggcaa aatttttggc aagaagcgcg gaagttttac
     8581 ccggcggtgt tgaagaaggc ataaaggaac ttgcaataaa acttcaaaaa gccaaagaaa
     8641 caaacactcc tttacgaata aaactcggaa tggatcccac acgccctgac ctgcatttag
     8701 ggcattgtgt agtcatgcgc aaattaagag agtttcaaaa actcggacat aaaattgttc
     8761 ttatcgtagg cggtgcaacc gcaatggtgg gagatccttc gggaaaatcc gaaacaagac
     8821 cctctttgac aaaagaagaa gtcgaaaaaa atgcaaaaag ttattttgaa cagatgtcaa
     8881 aagttgttga tattaaaaat gcgcagattg tcaacaacgc cgactggctt cacaaaatga
     8941 actttgttga cgttttaaaa ctgtcttcac acatcacggt cgcacaaatg cttgtaagag
     9001 aagattttgc aaaaagaata agcgaaaata aacctatttc cgtacacgaa tttttctacc
     9061 ccctaatgca ggcttatgat tctgttattg tagattccga catagaaata ggaggaactg
     9121 accagagatt taacgtgctt ttaggaaggg atattcaggc ggcttacggc aaaaaatatc
     9181 cgcaaatggt aatgcttttg ccgcttctgg aaggaacaga cggagttgtc aaaatgtcaa
     9241 aaacttaccc cgaacactgc atttccatta acgaaaatcc caaagacatg ttcggaaaac
     9301 tcatgagtat tcccgatacc ttaataatac gctattacac gcttttgacc gatgtctccg
     9361 atgaagactt gaaagaaatc gaaaatcaat taaaatcagg cgtgaatcct cgcgatatca
     9421 agctaaaact tgcccacact atcacacaag aatattgtgg aacagaaggt gccgaatttg
     9481 gcaaaaatga attcataaat gtcgtatcca acaagggaat accaactgat gttcccgagt
     9541 ataaactaaa aggtgaaaca aacattcttg atttgcttgt cgaattaaat tttgttacaa
     9601 gcaaaggtaa ggcaaaacgt ttaattcaag gcggcggagt aaaatttaac agtgaaaaaa
     9661 tctccgatat gaattatcaa attaataaag aaggaatttt acaagccggc aaaagaaatt
     9721 ttgtaaagtt gataaaatag aggcaaaatg attcacaaca atatcttaga acttatagga
     9781 aaaacccctt tggtaaagct tagaactttt aacaccaaag gctgtgaaat tgcgcttaaa
     9841 ctggagtttt tcaacccatc gggctcagtt aaagacaggg cgtcttttta catgctaaat
     9901 gaagcaataa aagcaggcaa aatcaacaaa aataccgtaa taatcgagcc tacaagcgga
     9961 aacacgggta taggacttgc catgtgcgct gcggttatgg gtttaaaaat agttattgtc
    10021 atgcccgaga gcatgtctat tgaaagaagg ctgctgatta aaggctacgg tgcaaagctt
    10081 gttcttacgc ccaagaaaaa aggcatgcaa ggtgctgtag ataaagcttt tgaacttgcc
    10141 gaaaaagacc cgaattcttt tataccttcg cagttttcaa atcctgcaaa tacgcttgca
    10201 cacaaagaaa ccacctcaaa agaaattttt gaagatacaa aaggcaaagt tgacattgtc
    10261 gtagcaggca taggaacatc gggcaccaca atgggacttt gtgagggatt aaaagcacta
    10321 aaaccttctg ttaaaatcta cggagtagag cctatggaaa gcccgctttt aaccgaaggc
    10381 agatcggctc cgcataaaat tcagggaatc ggcgcaaact ttatcccaaa actttttgac
    10441 aagaaaaaaa tcgacggaat aattaccgta aacgcgcaag attctttcaa atttgcaaaa
    10501 gagcttgcaa aaagagaagg aattttagta gggatttcct ccggtgcaat cttaaaggct
    10561 gctttagacc ttgccaagaa aagtgaaaac aaaggaaaat taattgtcgc cataatgtgc
    10621 gatttcggag aaagatactt atcaacggaa ttattcgaaa gttgaaaagt cgaacagtcg
    10681 aatagttata atgtcgttgc gaggagggca aagcccgacg aagcaatcca aaacaaccct
    10741 taagccattc acctattcac tttataacct aaaaaacaaa ggattaccaa atgctcacaa
    10801 gaaccataca aaccataaaa gaagacataa aaacaatcta caaaaaagat cccgctgcaa
    10861 aaaatttgct tgaagttatt ttttgctacc cgggattcca cgcacttttg atacacagat
    10921 tttctcataa actctgggta tggaaaattc ccgttatccc tcgcatgctt tcgacttttg
    10981 caagattttt gaccggaatt gacattcacc cgggagctac aatcggaaga aggtttttca
    11041 tagaccacgg catgggggtt gtaatcggcg aaacaacaat aatcggcgat gatgtgcttt
    11101 tgtatcaggg cgtaacactt ggcggaacaa gttcaaaacc cgaaaaacgc caccccacgc
    11161 tctgcaacaa tgtcgttgtc ggcgcaggcg caaaagtttt aggcaacatt acaatcggtg
    11221 aatactcaaa aatcggcgca ggctcggtcg taatcgataa tgtcccagaa catgccacag
    11281 tcgtaggagt acccggacat atcgtaatgc aaaaaataat cgacgaaaac ggagtgctta
    11341 tgcacaacag aattccagat ccgatttctt gcgaaataaa caggcttaaa tacgaagtca
    11401 aagagctcca agaaatcgtt aaaggcgaaa aaaataatta aaatttaaac caaggcggcg
    11461 cggtaaaatt accgcgccgc ctttatacat actctctctt aatatttaca tctgttttat
    11521 aacctgagcc agagcatcca caataatagg atcccacttg ctttcggctt catttttcat
    11581 aatttcaagt gcctgatttt taagcattgc ttttcgataa ggtctgtctg cggtcattgc
    11641 gctgaaagca tctgctaccg cgataattct tgagcctaac ggtattgaat ttcctcttaa
    11701 gccttcgggt tctcccatgc cgtcccatct ttctctgtga taatgaatat atggaacgac
    11761 ttcggacagg aagttgatat tcataagcag attaacgccg acattagggt gattttgaag
    11821 tttttcccaa tcctctttat caagttttcc ggtatgagaa aacaaggttt cgggcagggt
    11881 gattttacct atattttgta aaagccctgc ataataaatc aaatcggtag ttttttcatt
    11941 caggcccaaa tgtttgcata tcaagcgtga aagatctgcg gtattttgag agtgcgaaac
    12001 cttatactga cctttcgtat caactgcaga agcaagtctt tttacaaatg tctgctgctg
    12061 atcgcccaaa tcacctatta aagcggttaa ttcagctctc atatgctgca agtcggcaat
    12121 aatagaatct tctgatgcta taagttcatt tgcttcttca ataactcttt gaagttccat
    12181 tgaagtggca aaaagcgaag cggtagattc tatcaatctt aaatattcac tttcaaaaaa
    12241 tctatctaca taactttgaa ttactataac ccctactgct ttagcattat tgtgcatagg
    12301 aattgcggta aaaactttaa ctttatcttc gtttaatttc ttaataggtt caaatctttt
    12361 tgaaaaattg gggttcttaa cttcgacagt ttctttgttt ttaaaagctt ctgagacaaa
    12421 agatttatca tcaaatttat aacctaaatc atacttatac aaatcgtctt taatttctat
    12481 ggaagagccg accaaaagca aatctttgga agattttccc aaacctttgg cattttcttt
    12541 tgtcaaataa atatgacaaa cgtccatatc aagcatttga actatagttt ttgcaatcga
    12601 attgtatata acataatctt ctttagagct aaatcccaaa acgcaaagcg tattatcaat
    12661 agaataaatt gaaatcaatt cttttaactg acttttcaaa ctttcggctt tgtctttgac
    12721 attgtgtccg attttttgca ccaaatcttc tgaaatcagg tgttctgaaa taaagtcacc
    12781 caaattaagg gcaaaaatct cttccaacgg atcttcttct atcaaattca ttgaccgtcc
    12841 gaataatgat gctccggact ctaattctct tttgtccatg ccggtcactt tccctttgta
    12901 ttaccacata ttttacttat cggcacaacc tataaaaaac tttgatgatt tttataaaag
    12961 tttatacttt agtttgaaaa acttcatctt aaaagatgaa aaatccttta ttttcgcctc
    13021 ttaaattaaa tgtaacaaat ttttattttt ttaattttta aatcacatga ctaaattatt
    13081 aacatttgta aagttgagtt tttacaccct aaaaaacctt ttattacaag gttttacata
    13141 aaataatata tactttttat ctaaaaagga acactttttt ataactaaat gtttttataa
    13201 atatatcaga taattattat tttagttaaa ggagaaataa tttatggcaa gagttttgat
    13261 ttcaatgccc gaaaaatttt tagacgagat tgattcagtt gcgggtaacg aaaaccgttc
    13321 acgttcagag cttatcagag aagcacttag aacttatatt cacagaaaca aagtaagaga
    13381 aaacgttacg gcaacaaaaa atgcaactat tttagaggca ctactagact aacggattta
    13441 tcggaggaat taaaaaagaa ctcgcttaaa gcgggttctt tttttgtaat tttttaagac
    13501 ataaaaaggg ccggtttccc ggccttttaa ctaacacata cactaactaa catttgtcac
    13561 actaactaat tcctacccac caagttattt atattaagcc tttttctttt ttgagaacaa
    13621 agatataata cctgctccaa gtgcaactcc taatgctatt ccaagcggag gacaaagaac
    13681 tcctgcacct acgcatatcg cgcccgcagc tacaactcct aaggatccgc ctacggcttt
    13741 tgatgtcatt tgtgatttac ttagaggctc gcctgttatg tctgagatat tatcttgagc
    13801 cgtgcggctg tttccaaata atgttcccag tccgaaaaat cctttaccga aacctgacca
    13861 aaattgacct gtgccgtttt ctgctatttc atcagtcata cttttacctg tggcttgatt
    13921 atataatttt tcgacataag cctttaattg gtcatttgaa atcttgctga catcaattcc
    13981 ctgagctgct aactgttgtt ttgcagactg caacaactta ttgtaacagc tcataatttg
    14041 atcttgttca ttggctttta ttttagattg taaaatagcg atttgtcttg ttatcgcatc
    14101 ttcttcagaa gtaagatcaa agtttacgcc gttcatcctt ttttgatatt caacctgatt
    14161 atttaattga gacttttgca tatcattttg gatttcacct tgcaatccaa cataatcctt
    14221 tactcttttt gcatattctt caagagtttc tccttcttgc atcataggca tttgtccggg
    14281 atagccgtaa gaattttgta aaatagtagg accaaaacaa cctccgccta agttgtttaa
    14341 agtattaaag ccattattgt taaaaatact gcttgacaaa aaatcgtcat ttccgtaaaa
    14401 tatgtcgtcg ccacttaaat taccgttgtt acaaatccct gattgaggat aataaggcgc
    14461 aaaagtacca atacttgctg tcataaccag acctccaaat taaataacta aacctacctt
    14521 acatatataa aaagtttttt tattaaataa ttgcgcatgt ttagattaat tgttaagaaa
    14581 tgttaactta cgcgattggt aaaacatgcc aaaaaatggg cgcaggcaaa tgcctgcgcc
    14641 cattttttat ttattaatta gatgcagata aaactttatc aatgagcccg taatcgacgg
    14701 cttcttgtgc ggacatgtaa tagtctcttt cggtatcttc ttttattttc tctatagttt
    14761 gacccgaatg ttgcgccaaa agtccgttga gcatgttttt cattcttata atttcttttg
    14821 cttcgatttc tatatctgtg gcttggcctt tagccccgcc aagcggttgg tgaatcataa
    14881 ttcttgcact cggaagtgcc attcttttgc ctttagttcc tgcagagagc aaaaatgcgc
    14941 ccatacttgc ggcttcgccg atacatattg tggaaacatc tgctttaaca agcttcatgg
    15001 tgtcataaat cgccattccc gctgttataa ctccacctgg gctgtttatg tacatcataa
    15061 tatctttttc gggattttcg ctgtcgagca acaaaagctg tgctactatt gaatttgccg
    15121 attcatcatc aatttcgtcg cctacgaata taattctttc tctcagcagc ctcgagaaaa
    15181 tatcaaaggc tttttcgccc cttgaagaag cttctattac tgtcggaaca taatttaaga
    15241 cctgaagatt atccatttat tttctccaaa atataataac tcttaataaa aattatacta
    15301 agaatatttt attttacaaa catcctcaaa agtagtataa ttcacatatg tattgcatag
    15361 atgacatagt ttacatagaa gatgatgacc aatgtatcag ttgcgaaaat tatgcaaggg
    15421 gcgtggcatg cccgttgatt gaagcattgg gaatgggcgt tgtctatctt gaagacagtc
    15481 ttaccgtaac aaattgcgga ttttataaag agtttaagag aaatttaaga attgtgaaaa
    15541 acgaacaatg agaactgtaa cattaatccc gggcgacgga ataggtcctg aaataaccga
    15601 ttgcgttgta aaaataatca aaaaaagcgg cttagaactc aactgggatg ttcaaacagc
    15661 aggaaaagac gttatcaaaa aagaaggaac acctcttccc caaagagttt tagaatcaat
    15721 caaaaaaaat aaaattgcgc tcaaagctcc tgtaacaacc cctataggag aaggttttcg
    15781 ctcggtaaat gtaactttga gaaaagaact cgatttatac gcaaatttaa gaccgtgtaa
    15841 aaatatcaaa ggcattaaaa caaaatttga tgatgttgat attatcgttg ttagagaaaa
    15901 caccgaagat ttgtatgcag gaatcgaaaa acaaatcgac aaaaacacgg ctcaaagcat
    15961 taaaataata acaagagaag cctcagaaag aattgcagaa tttgcttttg aatatgcgct
    16021 caaaaattca agaaaagaag tttgctctgt aagcaaagcc aatatttgta agctttccga
    16081 cgggcttttt ttagaaagcg taagagatgt tgcaaaaaaa tatccgcaaa ttaattacaa
    16141 agaaattttg gtagataatt tgtgcatgca gcttgtacaa aacccccaaa aatttgacgt
    16201 tttagtgctt ccaaaccttt acggagatat tgtatcggac ttatgcgcgg ggcttatagg
    16261 aggattaggc gtagcgcagg gcgcaaacat aggctttcat catgcggttt ttgaaccggt
    16321 acacggctca gctcctgaca tcgcaggaca aaacaaggca aaccccactg cacttttgct
    16381 ttgcgcgata gaaatgctca aatacatggg cgaaaatttt tatgcccaaa aaatagaaca
    16441 ggcactttta aaaacccttc aaggcaaaat tttaacaccc gacttgggcg gaagcgcaac
    16501 atgtacaaaa ttcacagaag aaattattaa acttttgtaa accgccacaa tccaataata
    16561 ataataataa taataataat aataataata ataataatgt aaaaactttt gcccaccacc
    16621 tattttagtt gtatgattaa ttagaattaa catcaattta aagaggtttt tatgagagag
    16681 aattttagag gatttacgct tgcagaggtt ttgataaccc ttaccataat cggtgttgtg
    16741 gcagcactta cggtgccgaa tttatacgcg tattatcaaa aaacacaata tgtaacccaa
    16801 ttgaaaaaaa tttatactga aatttcacaa gccctcaaac taatgatggc agatgaaagt
    16861 ataaataaaa taaccgcttc tgatactttg tattgtgacg agactgcagg atgtatttgg
    16921 gacgaagctt atcaaagggc aggaaatgat tttttaaaaa aatattttaa ggttataaaa
    16981 gattgtggca gggaaaacct atcaacatgc tttgcggata actataaatc aattagcggc
    17041 aatgaagccg gtgggatcgg atgctactat agtgttatta ttgccagcgg tgcaagtatt
    17101 tgtatatccg gtcaatcagg cacctacaat gcaggaactt ttatcgttga tataaacgga
    17161 ctaaagctac caaatgttgc gggaagggac ctttttacct tttcatatta ttatgacggt
    17221 tctttagatg aaagtattac tcccgaatgc agaaaaggaa ttgctaatgc aagtgggctc
    17281 tgttctaact atggttctaa tgcgcaagaa gcaagagagg aatatttcaa taatggcaga
    17341 ggagggcaac caggttgttt aggaaatagt tacagcgcta ttggttgttt cggtaaaata
    17401 ctaaacgaca actggaaaat ggattattaa tttccatcgt tgcgagagat aaatgacttg
    17461 atttattatc accgctcgcc ctctcgtcgt tgcgaggctt tgaaaaagcc gtggcaatct
    17521 aaaaatcctt ggattgcttc gtcgccctgt aaaacagggc ttctcgcaac gacacaaaaa
    17581 gcagaatatc acaatgcaac ctcttgcttt cctatcgtat aaacttctct acaatcgcta
    17641 aatcttcggg attgtcgatt tcaaaaaagc tttttgaatc catttcccaa aaggtgattt
    17701 tgcctgaaat cctgcatttt gattttaaga tattttctct tgagcttatg taaaatgccc
    17761 cgttttcaat catatacccg tcccaatcct gtcttctggg gcgtttttgg gggttataat
    17821 ttatgggctt gccttttata tcccataaaa attgataatt acgaatggct gaaaccaaag
    17881 aatccgaacc ttcttttttc atttttttaa gcgcaccttc caaatccgca gaagcagtca
    17941 atggcgaagt cgcctgcaaa aatacgactt tttcaaactt atgattttta caaaattcaa
    18001 tcaacgcgct ttctgaagtg gcggtgtctg tagccgtttc aggcgagcgc gaaatcactt
    18061 ctacattttt aaacccaaag ccttcaacaa cttttttaat ttcattactg tcggttgaaa
    18121 caaagacttt ttcaatggat tttgagccga tacaggctga aatagcccaa taaataagcg
    18181 gcttgccggc aagcggcatt atatttttgt ttttaatact tttgcttccg cctcttgcgg
    18241 gaataaacgc aaccgttttc ataaattttc cacatgtccg atataaggga tatttctaaa
    18301 atgaccttca aaatcaagcc cgtaaccgat tacgaatttg tcatcaacat caaagcctac
    18361 aaaatcaggc tcaatctgag ttttcctgaa acatttttta tccaaaagcg aacagaattt
    18421 tgtcgttttt gttttgaaat tgttatttat aaaatcaagc agaaattttg ccgtaagccc
    18481 tgtgtcaaca atgtcttcca ccacaagcac gtttttgccg tttaaatcag gaagcgaaat
    18541 atcaaccgca ttaacttttc ctgaagtagt cgtcgaatga ccgtagcttg aaagcctgat
    18601 aaattccatc ttcaaaggca ttttaagata tttgaccaaa tcgacggcaa acataacagc
    18661 acctttgaga acgcaaacga cataaacttc ctcgtcttta taaatcgaat tcaaatgaag
    18721 agcaatttct tttatcctgt gttgaatttg ctctgccgta aataaaattt ttaaatcttc
    18781 ttttttaatt tccatatctt taacctgcat atctcttatt taccccctac atttacccct
    18841 aaattctaac acatgagagg gtttatttac aactttaaat ttatcactca gtcctacgcc
    18901 gataacccaa aagacttccg aaccactgca gagcataagt agtgagtctt tttcatgctg
    18961 ggcaacccct tttgaattaa gatatttttt aagcttcatt gtgcctttca tcccgaacgg
    19021 gttaatgaaa tcgccttctt tccttgtccg cagttcaaaa tcaattttga ctttgatttc
    19081 gacgtaagcg caaagggcgt tttcgcaggg aaattcaatt ttttctgcgc ctttatattt
    19141 ttgaagcgaa aacgcccctt cgatttcggg gattttgtat tcgccttctt tgtttatttt
    19201 gatacttata ttttttttat tttgaagttt atctatcaaa tacatgtttt ctttattgat
    19261 aaaaagccac aaatcgcttg taaccgaata tcttgaaccg gcttttgaat tccagttttc
    19321 ttctataaaa tcagaaatac tttttatttt tttaaaatcg taatctaaat tatgctccaa
    19381 aaccctgtcg taaatgattt tttgccttac ttctttggat aaatttttaa attttggagt
    19441 taaaatttta ccttcgcaat aaatttccga tttaatcaag ttcaagtatt cttcgactat
    19501 tttattttgg tttgaagcga tttttgcaag cgaaactatt gccattgggg cactgtaatt
    19561 aatttcttta aaaagaggca tgattttatg cctgataaaa tttcttttat aatttgtatc
    19621 aaaatttgaa gaatcggtat tagcgacaag cccccttgaa atgcaatatc cttcgatttc
    19681 ttctctggca agccccaaca aaggtctaaa gacactgtga ccgtcgattt ttcgctcggg
    19741 caaaatcccc tgcagtcctt taattcctgt tcctttaatg attctgtaaa ttaaggtttc
    19801 ggcattatcc gatgcgctgt gggcagtaaa aatcgaacaa tttttatatt tttttgcaag
    19861 acttacgaaa aatttgtatc ttgcttcgcg cgcggtattt tcgcttttat ctacagtatt
    19921 ttcaagggtt tggcaaacgt attcgatttt atttttttta caaaatttct cacaattttt
    19981 ttcttcctgc agggattctt cgcctcgcca gttgtggtta agatgcgcgg cgacaacttt
    20041 aaagcggtgt ttttttgaaa gtgtttttaa aatatcaagc agggccattg aatcgcatcc
    20101 gccggaaaac cctaccaaaa aagtattttc ggaagaaatt aaatcatatt tttttaaaaa
    20161 attttcaaca atttcaatca taatttgagt aaataagtta ataggtaaat aagtgaatag
    20221 gtgcaattat aataaattta acttttcaac tattcgactt ttcgactatt caactatttt
    20281 cacctttcaa tttccttaag tatcccttgt tttatttcga cggcatcgac gccgagctgc
    20341 tccaatgctt tcatagcaac cgactgcggc tccattgtta ttgcataaag cagatgttcg
    20401 gataaaattc ttgatttaca atgttttttt gctttttccc aggcgatttc caaaactttt
    20461 ttggcgcgtt tcgtgaaaat aatttctgtt tcgtaatact cgttaccata gccgattaag
    20521 ccttctataa cctgtcttgc gtcttttata tttatttcaa gttcattgag aactttaaac
    20581 ccgacgccca tgccttcgtt taaaatgcca agcaaaatca tttccgtacc gacgatgttt
    20641 ctgccgagcc ttcttgcctc ctgttttgca agcttaagga ttgtaagtgc ctgcggcatt
    20701 tgcttttcga taggtttaac aatttcttcg ccaagcgaat tttcatcaac attgagtgct
    20761 ttaaaaatgt cgtatgcaac gcctttttta gcctttagaa gtcctaaaat catatgttcg
    20821 ggcttaattt caagagagcc aagttctttt gcggcttgaa tcgctaaatt catagcaaca
    20881 aaagcgtttg gagtaaaaat aactttttta ccttcaaatt cagaagctct ggaagactgc
    20941 ttagacagtt cattttcaaa agcttctaaa gtaattccct cattgtttaa aattttaaca
    21001 atatcagatt ctttatcctc taaaattgaa gagattatct gctcggtgcc caaaatttca
    21061 taatttgacg ccgaaagctt cgaaaccgcc ctttcaaata ttttagaaga ttcttcgtac
    21121 tcaaaaagac aatccgccgt aagatatgaa gactcaaact cttttgtatc ttctatttca
    21181 ggatgagaaa gctttttaga ttttttttcg acaagtttgt ataaaatttc tctggactcg
    21241 taaatattaa agttaaactg ttccaaaatc cttcttatat aagaagtttt atcgttaaga
    21301 actgcaataa aaaggtgctc aaacaaaaca aaactgtttc ctgatttgtt agctaaatca
    21361 agagttcttt tcaaaagctg tttaaactcg ttactgaaag gccttgcctc ttgagggctt
    21421 tccgcctttt caaacctcaa gcgttttcca accgcctcct taagctcttc aaaattaata
    21481 ttttgcgctt taaaaatttt ttgagaaaca cctttagcct cttttgcaat cgccagaagt
    21541 aaatgctcag gctgaactaa tgcattgtcc attaagctcg cctgattttg agcctctgtt
    21601 actgcattta tggctttttc ggtgaatttc tcaaacatta gtcttctaca tctaaacttt
    21661 caataaattc tgcgacttta tcaaattctt catcactttc aattgcttca aaaagatagt
    21721 cttcgccgtc ttgtttcaaa cgcatcaaaa ccacctcatc atcgtctttg gggttttgag
    21781 caggaagcaa aagtgcatat tccttgtcct caaattcaac aatgtcaaaa agttcaaatt
    21841 taataatttt cccttcttcg tcgattgttt ctataataaa attttcctct ttagtttttt
    21901 ctttcattgc tgctccttat tttgactgat aatttattat tttattttgg cgggattgct
    21961 atcccgccct acttttcaat aacaattctt taattataat tgcaattatt cacttttatt
    22021 tgctgttcac ggctcactgt tcaccgttca cgactaagat attgttccag tataatacaa
    22081 gcactttcaa tatcaactag tcctttgtct tttttaaagt taattttttt ctcgcgcaaa
    22141 ttttcttcgg cttgctggga agtaagtctt tcgtcttcaa aaattatctc aaattcgttt
    22201 tcaaaattct ttgcaaaatt aatacagtct tttgcctgag aaccttttgt gccgtccata
    22261 ttcaaaggca cgcctatgac aattttttta actttatttt cttgagcaat ttttttaatt
    22321 ttttctaccg cgtttttctc cggaactctt tctatacaac tgtttccatt tgccaatata
    22381 agcaaaaaat cacttagggc aacgccgatt ctttttgttc cgacatccaa cgccataact
    22441 ttatacattt tcgcgcaccc acaaattttc tatcatggaa atgatttttt ctatttgaac
    22501 gacagaaagt cccttttctt tttgcttgtt gcgaagagca aatatgtttg ctgtcttttt
    22561 ttcttctatt tgcttaaatt tttgcaatac gtagtattcg taaatacttt ttctgagcat
    22621 attttcgtat aatttaacct tgttttcctc gtcaaatctg agtgcatata aaatttgtgc
    22681 gtcaattgaa tcttttttca aaaactccag ataagcacac atcaaaagcc tgtcttctat
    22741 ggattttcgc tgtgattcgc aaaaaatatc agacaatttt tcttgaattt gcaattctaa
    22801 atcaacctca aaatcatctt caataaattt ctgattaagc ccttctacaa attttgtaaa
    22861 ctcttgactg tattcggtat ccaaaaacca cctttgaata aaaggcgcaa aataaaattt
    22921 ttccatttcg ctatctgaaa gttttcgttt tacaaaattt tcatccaaga ttttctcaaa
    22981 attttcagtt tcttgaatat ccgataaaag agttttccag caaagaaatt cataagaaat
    23041 atctccgccg gtttttctgg taattgtttg agcatttttg agaatatatt ttacaaaaga
    23101 aggcggaatt tgagtatgct cgtcaccgtc gaaaaatctt tttactatac gctcaaattc
    23161 cgcctttgaa atctcgttaa aaccgaagca atctacaatt ccccagcctg aattaataac
    23221 aacagcaaac atctgaattg tttctatatc tcttattctt gaaaaaataa ccgcctgatt
    23281 tccatgtccg tcgggatagc tgatataact ttcaaaaggc gaagatgcag ataaaatttc
    23341 tttgtaaaat tcaatcgtat tgtcttgcct gacccctgct attttaaggg tggaaatatt
    23401 ttttttgatt agcaaaaccg tttgctcatc ttccacaaaa tctaatgctt caatcaaagc
    23461 atgaagcgca agctgagatt tggtttcgcc taaaattccc actgatattt tgcccaaatc
    23521 ggattcggga gaatacaaaa acaaaggaat taaaatattt gcaagctcat cttgagaata
    23581 atcttcaccc aaagatttta ttaaaatttc cttgtcttta tcaggcaaag acttcaaaaa
    23641 atccaaaaaa tcaatttggg cttcaggatt tataattgca acatgaagaa gctttttcgt
    23701 atcggcatct attacgtcat cgggattttc aaaatattcg cccagctttt cataatttac
    23761 ccgattgccc aaatcacgca aaagattcat tacaactgat tttattccgt catttacttg
    23821 agggcttttt aaaatatccc aaaggggttt ttccaactct tctttttcgc aaagccgcaa
    23881 aagcatataa caaatcaaaa cagcgcgctg ctcggtcgct ttcaacaatt cttttatcaa
    23941 aacatctaaa attgcccgtt tatcctgctg gattaacaaa acttccatta aagcttctgc
    24001 gccggaaact tcaggcaatg tctgaagctt cgatagtaca gtcaaaactt ctgcctttat
    24061 ttgaagtttg ttcaattgat tattgctcat tttctgctct taccccaaaa tgcatccaaa
    24121 atcatctggg cttcagccga tggcattcta tcatttaaaa ttaagtattg aacggcaatc
    24181 attgtacact ccgagcaaat gttaaccccc gggccttgaa ccatttttat aacctgagta
    24241 ttgggcctgt tacaaaaact gcaaattaca acttcttgtg tttcttcttt tttcttttca
    24301 ggttttgatt ttttagaacc caattttact actttatcag acataaatac tccttattaa
    24361 tttaatatag ttaatatcct atttactatt tactatttac tgatatattg taacttaaat
    24421 tataaaatat tgccaaatat taaattttat tataaactgt tttggggaca ttggagaaaa
    24481 ggggtttatg aaaaaagcat ttttacttgt aacgatactt tttgtttccg ttatttcaac
    24541 ggcatgtata aacaatttag cggtccaaga gcttaacaac aaggcaaaat cctaccttga
    24601 caaaggcgac tacgaaaatg caataagcag gcttaaatca agtttggact tagacagttc
    24661 tttttttgaa acccattata atttaggcat tgcctacact caggctgaaa aataccccga
    24721 agcggttgaa acttttgaaa gcgcaataaa aatcaaacct gactttgcag atacttacta
    24781 ttcacttgca gttgcgcagg aaaattatgc aaaaggaatt attgacggca caataaaaga
    24841 cgaagataaa gacgaagata aagagcaaga agacaaaatt gacgccgaag aagaaaccca
    24901 tcaaaacaaa aacttatcgg cacaagaaaa aacaaaagtt tgtgagcttc ttgataaagc
    24961 cgtacaatct tatcaaacat accttgaaaa aggtttggaa ataaaagaca aagaagaagt
    25021 tgaagaaaag atagagtatt taaaaataca aatcgaaaaa tactcttccc aaccccctct
    25081 aaaagaaggt taaaatgttt gtttcaccag gcgacatatt tttttcaata ggtgctttca
    25141 gcatatacta ttacggaata ataatggctc tgtctctttt gctaggggtt ttggtagcaa
    25201 gcaacattac aaaaaagctt tatccggaat taaacagtga aataatttat gacatttcac
    25261 cttatataat tatgtgcgga attataggcg caagacttta ttactgccta ttaagcagcg
    25321 aatattattt ggctcacccc aaagagataa ttcaaatatg gcaaggcggg atttcaatcc
    25381 acggggcaat tataggcggg cttatcggcg gaattttata cgcaaaagca aaaaagttgc
    25441 caattctaaa gctttgtgat attttttctt acgggcttat tttaggtcaa gcacttggaa
    25501 gatgggggaa tttttttaat tcagaagctt tcggacttcc tacagataat ttttttaagc
    25561 tttatatccc gatttacaaa agaccgattg aatacctgca gtataacttc tttcacccta
    25621 cgtttttata tgaaagcatt ttggacataa ttattttctt cattttgctt tttgttgtaa
    25681 gaaaacttac aaaaaatatc gacggtataa tattcttcag ctacttaaca atgtattcgg
    25741 ttgccagaat tttaattgaa caaataagaa tagacagcgt tttaaatatt tttggaattc
    25801 ccatagccca aattgtaagc cttgctttat tttttgttgg aataatagga atttttatac
    25861 taagattgag aaatgaaagt tcaagacaaa ctgactcttg atatcgaaaa actctccaat
    25921 ctcggatttg gaatcgcaaa acacgaaggg cttgtaatct tcgttgaaaa cacttgcccg
    25981 caagataaag tgctggctga aataaccaaa ttaaacaaaa attgggcaac tgcaaagatt
    26041 gttgaaataa ttaagggctc ggcgcatcgc gccgagccct tctgtgcaat gcaaaaagta
    26101 tgcggcggat gtcaattaca gttcatcaat tacgattacc agcttgaatg taaaaaggaa
    26161 ataattgaag acgcgataaa aacaatcggc gggttgaatg ttgaaataaa aaacccgata
    26221 gcaagccctc aaattaaaaa ttacaggcac aaagtccaat atcctatttc acagacaaaa
    26281 gtttcgggaa gaattttagc aggatactac aaacccaaat cgcacgaaat tgtaaatata
    26341 aaacactgtc ccattcagcc tgagatttgc gacaaaatta ttgaatttat aagaaacact
    26401 gcatttaatt tcggaatttc cggatacaac gaaaaaaaac acgcaggaga cttgagaaac
    26461 gttgttatga gagtttcggc atcaagtggc aaaatccttg taacgctcgt ttttaattca
    26521 ttacccctca cccccgccct ctcccgcaag gggcgaggga gttggataaa agaacgcatt
    26581 tacccctttg cccaaaatat atttgataat tttaaagaag taacgggtgt ttgcataaat
    26641 tttaattcta aaaaaacaaa tgttatttta ggtgaaacaa cagaacttgt agtaggaaat
    26701 gacttcataa aagaacagat tttggataga tccttcaaaa taggtcctaa tactttcttc
    26761 caaattaatc ccaaaagcgc gcaaaatatt tttaaatttg taaaaaaata tataaaaaat
    26821 aattatgaac agccacttgt attagatgct tatgcgggcg taagctcttt cgggatatgc
    26881 gtgtcagatg tttgcagaaa agttgtaagt gttgaagaaa ataaagaatc cgtaagcctt
    26941 gcccaagaaa ttttaaatct caatgacatt aaaaacgttg aacttcacaa tatggatacg
    27001 acaaaatttt ttgaaaaaga attgaagaca aaaaaacgca aatttgatgt tataattctt
    27061 gatccgccta gaaaaggttg cagtgaagaa agtttagaat atgcgcttaa tctttgcaaa
    27121 gggaaaataa tttacgtaag ctgcaatccc gcgactcttg ccagagattt aaaatattta
    27181 acttcaaaag gtgcaaaagt tgaatttatt cagccttttg acatgttctg ccacacttat
    27241 catatcgaaa gcgttgccat aattaattgt aacattttat aaacatttta atattttaac
    27301 ggcaaaaaaa aaaattagag cgtttaatta aacatagctg tattgtttta tcagaaagtg
    27361 tggtaaaaat gaacattcaa agtataagtg catttaacag tttaacatca aagaaaattg
    27421 ctttttcaaa atcaaactca aatgttgaaa aattgcccga cggacaagtt attgttacag
    27481 attattgggc ggacggaaaa actccgatga aaagaacctt atatgcagat gaaaccctga
    27541 aggttcctgt cagatttgcc ctttatagag ccgacggaac agaaagaatc gttcatgatg
    27601 ctatgcctat ggtaaataaa aacaaccaaa agcctgtttt taaaataaac aaagaaaccc
    27661 tgcccgacgg acaagttatt gttacagatt attgggcgga cggaaaaact ccgat
//
LOCUS       DAOQAU010000018        26219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1846_length_26219_cov_5.675814, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000018 DAOQAU010000000
VERSION     DAOQAU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 26219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26219
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1846_length_26219_cov_5.675814"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKI94_04185"
     CDS             <1..943
                     /locus_tag="PKI94_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25972.1"
                     /translation="QERLEIEGSFPRIIEGICQGFSNLKSYYQGLNTQCEGNEVNIDS
                     ISENFTNFDALIAQLITKMQDFNEAMAVANEAHQTVRERVEEYGIAAYLAYQAYPDDQ
                     SYQDEANTAYAAWMAAGTAAMAADGNLDNIKNDFFGIITDGLTPLINNINASFIQATQ
                     PKPPTEPPTDPDNTYTYDAGRVFYYSNIFNEMLKGYISVEDEKFNDATWIHDQIKNGN
                     IHMEEYSESANGGQGGFVETSIAQDSSLYEESYEKNNAAAQAEYDRLISDLNYKEKQL
                     DMKLNTINTEYNAAKEEYESVKKTQEKSIDRTFKIFG"
     gene            1088..2179
                     /locus_tag="PKI94_04190"
     CDS             1088..2179
                     /locus_tag="PKI94_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25973.1"
                     /translation="MGMAASQARLLSLTARLSDLEFQAMQLTNAKIQLSRSTEAVASE
                     YTNALNNKIFKIKNTNTPLTASSLTGFQTLPSIEAQRILVDTSDRILVSGTVARAFDT
                     AGGSCERFLNQFGYTSLHNPPNIGGDSDPTGGNNTTEIIKGVSESTTNSSTETDVYDI
                     LSMPATSAANNNDNNSGSGVNPGNNWGTSSGNTWGTGDGNTPSTPPPPPTVDPPPPTV
                     DPPPPTVDSRYTYDADAVQYYTNLFNQIQSHGYVTTDDQNLSNPEWLYEQLFNGNLRM
                     EKLTSTGHAQEDQWNTDGLLEEVYDTSDDARAELKYEVETTKIQNQDKKYDLELKQIE
                     TEHTAIQTEMESVKKVIDKNIEATFKIFA"
     gene            2470..3747
                     /locus_tag="PKI94_04195"
     CDS             2470..3747
                     /locus_tag="PKI94_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNW25974.1"
                     /translation="MERFFGLIGILVIFLIAFLMSNNKKLINIKTVGMGFLLQILLAV
                     FVLKTPVGQAIFYDIGLFIQKILQFANQGAYFLFGPLSDNIKLGNVFGKGSIVFAVQL
                     LATTIFVSVIVNILYYYKIMQRVIAILSKGMYKLMNVSGAESFSNVASAFVGQIMAQI
                     MIKPYLPNLTRSELLASMTGSMACISGAVMAIYIGMGIPAQYLLAASIMAAPGALVIS
                     KIVYPEDGTPQTQGEVKISKRRTHVNVVDSIARGASDGFSVALKVFAVLIAFVALIAM
                     IDWLLGKTGVFLHNALHINLSFIGLDIDNLSMKAIVGKLFGLFAYVMGVPLKDSSIVG
                     SLMGTKFILNEMLGYIDLISVKNLIADKSFIIASFALCGFANISSIAIQIGGIGEIAP
                     NQRKNISRLGLRALICGTLASYMSACIAGILLG"
     gene            complement(3708..4493)
                     /locus_tag="PKI94_04200"
     CDS             complement(3708..4493)
                     /locus_tag="PKI94_04200"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNW25975.1"
                     /translation="MLLTVDIGNTSITLGIFYGDALVEEFRLASDRDLSQAEYEVLLK
                     TLLKSFKIEGCIIASVVEELNSKFKFALENVLGISPMFLTHEINMGVKIVSDTPQEAG
                     ADRIANACAAFMLYKKPCIVVDFGTATSFDIVNKKGEFVGGIIAPGLNLQMNVLNKFT
                     SKLPKIEVAISNCAIGKTTQDAILSGVIRGSAAMIDGLVEQCEKELGQKAVIIATGGY
                     SGLISSYMKRPFDFINPTLTLEGLRFLYNLNQPSNIPAIQADM"
     gene            complement(4552..4713)
                     /locus_tag="PKI94_04205"
     CDS             complement(4552..4713)
                     /locus_tag="PKI94_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25976.1"
                     /translation="MGFAVDKIGAKAPVFKGLEEKEEPKKDKTAYAQSPSIFGQGQPP
                     QTTGTGFMA"
     gene            complement(4822..5202)
                     /locus_tag="PKI94_04210"
     CDS             complement(4822..5202)
                     /locus_tag="PKI94_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNW25977.1"
                     /translation="MKFLHTMIRVKDIEKSLKFYSELLELELVKEMRLGDCNLFFLSD
                     KHGEAQIELTFNDETPEEGYQVGSGFGHFAFEVDSMNEFDKKLKKMGLEYLYEPFNIP
                     NSKSLIAFVKDPDGYEIELVEKGL"
     gene            complement(5325..5810)
                     /gene="ispF"
                     /locus_tag="PKI94_04215"
     CDS             complement(5325..5810)
                     /gene="ispF"
                     /locus_tag="PKI94_04215"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNW25978.1"
                     /translation="MQKFRIGTGYDIHQLVDNRALIIGGVKIPYEKGLFGHSDADVLI
                     HAIIDAILGALALSDIGTLFPDTADEFKNISSLILLKKVYELILKKGYEINNIDSNII
                     AQEPKMMPYIPQMRKNLADVLGVDFDLISVKAKTNEKMDAVGRLEAIEANAVVLLTKS
                     K"
     gene            complement(5797..6606)
                     /locus_tag="PKI94_04220"
     CDS             complement(5797..6606)
                     /locus_tag="PKI94_04220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNW25979.1"
                     /translation="MADIKLIATDIDGTILKYNFEFNQEVKDCIKGLCQAGIKVVLVT
                     GRMHAATDYIAEELGLDTPIISYQGGMVKHNDEILYQRTLPITETREILNWAKQNDVH
                     VNLYMNDELYVEKDDEIVRRYTGERSTGFIVKSFEGLDLHDLNKLLLIDFNNHQRVTD
                     WANYLSKNYPQLHVVKSTPYFCEVCHGEATKFCAVEFLRKHYGFKKEEVMTIGDQNND
                     IELLAAGGLKVAMGNATPELKAVAEYITDTVNNNGFVKAVERFVDAYNAKV"
     gene            complement(6615..7163)
                     /locus_tag="PKI94_04225"
     CDS             complement(6615..7163)
                     /locus_tag="PKI94_04225"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNW25980.1"
                     /translation="MQKFYEKTLKSSHIFKGIVFDVKKDDVELSTGEKTIREVVNHPG
                     GVVILALKDENTILLVKQYRYPLKLVSLELPAGKLDRVENPDLAAKREFEEETGFVAK
                     KWDKLGIAYSSPGFCDETLHFYLARDLEYKKHSPDDGEIIEYFEYNLGKVFDMINSGE
                     INDAKTICAIMRAGLKGFIKLP"
     gene            complement(7171..8385)
                     /locus_tag="PKI94_04230"
     CDS             complement(7171..8385)
                     /locus_tag="PKI94_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25981.1"
                     /translation="MSEDIVKKLTGKNPKDFEYAAAHIIDNSDVKAFSVLVEKSDFLF
                     DFIKHNVAKRLENAVNKNNYLNLLNFLKVYSSDYEEVIINGLTKFADEDLTDKMLEML
                     ECGSNAQKAYCAKYFSFINDTLAIDLLRKYSYSDFDPLAMNCATALSKMKDDFSYNLA
                     LQKLESDDEFEKISAVRFLISYNDTGAIENIFKTMKKSSMPENIASEISYLQSFLEFF
                     DTDLKYDTILAVNHIINGLGEIVSLSQIFDFQLYDVLERLIELQKEEKSPKIAIVLLN
                     AKLKFEQLTENDEYIFDEDKDTKNEVFEIKTLLNSQFELFWNIQKDLALNELDQLSDF
                     VFPALDLIQELGMTEAFDKLKVLLNSPNQTIILKTVEVIKSLGKLEEINFENVLSKIT
                     DGNIKSIIKALF"
     gene            complement(8389..8976)
                     /locus_tag="PKI94_04235"
     CDS             complement(8389..8976)
                     /locus_tag="PKI94_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNW25982.1"
                     /translation="MVEEKILNSDGKPENEQPSIMVPVDDLDIVSDSSPDTVDELAME
                     LATIQQSAKRFAIIGSRNLPITHQQLIETLAMALVMQGSTIVTSGGSSGTNAAAIRGA
                     MKANPDKLKVILPQTIGQQPSDVQDQLIGVPNIIEHPDRAMMTLADASRVCNREIIDD
                     CNQLICFLSHTSKTLHKAVEYAEEAHKVVTVFYLD"
     gene            9175..11649
                     /locus_tag="PKI94_04240"
     CDS             9175..11649
                     /locus_tag="PKI94_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNW25983.1"
                     /translation="MKDILDNLNKEQIEAVKTVKGPLLVLAGAGSGKTRVLTSRIAYL
                     IKSGVRAKEILAVTFTNKAAKEMRERLASMLGDEIVKYMWAGTFHSICGRILRQDIEN
                     YKFQSGKFLDKNFTIYDEQDSLAVIKQAVKKLNLDDKIYAPKLIKAIISDSKNKMQDA
                     YSFSTFARDFKSQKIATVFEEYENILNNNNAMDFDDMLMLAVKLLEQNPQIREKYFSR
                     FSHILVDEFQDTNQAQYRLVNMLYTNLSDKHMENRSLCVVGDIDQSIYSWRGADFKIL
                     LNFQRDFRNTKMIKLEQNYRSTENILNVANAIIENNEERVEKVLYSQKGGGEKIQYFE
                     AQDESDEANFIAQTIKETSPDFNQFAVLYRTNAQSRALEEACITYGLPYRIYGGLKFY
                     DRKEIKDIIAYLNLIHNTDDSISFKRIVNVPKRAIGDTTIKNLQMIADQKDISLFEAV
                     LQLDEVEGINSKTKEKLKKFTDLILDFKKVQPKYNLREFVSHVIEKSGYLRELQEERT
                     PESESRIENLQELVNVAGEFEPEDINNAYSEFLAQIALVSDIDSLDNISNNVTLMTLH
                     SAKGLEFPTVFVAGLEEGIFPHQRTFNSPSELEEERRLMYVGVTRAEEKLYLTSAKRR
                     QMWGEFKYYNPSRFLEEIPQELLESSISQNYETQATFTSAANKIKGVNVGVKDYKAPK
                     AQPSVPSDRFGYIPPSSGFGKNFVAPQKRKNNDISSKSILVKSSINKEKEQEKIDKFF
                     KDNAIKRMLAEKKKEEAKKNAEAQKKFNSVPYFFNEGERVFHEKLGIGHIEAVTQINE
                     STMYTIDFGDKGKKAMDAAYANLKKF"
     gene            complement(11673..12062)
                     /locus_tag="PKI94_04245"
     CDS             complement(11673..12062)
                     /locus_tag="PKI94_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25984.1"
                     /translation="MQINGISNFQNKVNFTSHVFMNSEFLKYARQNPSDKGAWNEVIH
                     AMRHNGDKYVVNIFKAKSSLPDEFEGTGIAPKDVFEVSIYDPELNQTKNVIKTPDSGI
                     FDPLALKTIYEQEDEYFHKQKTHLTWV"
     gene            complement(12153..13190)
                     /locus_tag="PKI94_04250"
     CDS             complement(12153..13190)
                     /locus_tag="PKI94_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNW25985.1"
                     /translation="MQYKDYYEILGVKRGATEAEVKSAYRKLARKFHPDVNKSKEAEQ
                     KFKDINEAYEVLSDKEKRQRYDSLGANWQEGANFTPPPGFEQFNFNFGQGGRQSQTFG
                     FDEMGGFSDFFSSLFGDMMSGQARQSSYSSRGRNIYDDLGFSQQAQSRQSPKAQNLDI
                     TQNLTITAKDLYNRKPLPVRFKIMEKCPHCPPRGGFCPSCGGTGIISEQKGVNVKLPA
                     EVTDGQKIRLKGEGKADAYGRKGDLYLKINIKDNEYQVEGINLAKEIEITPSQAVLGC
                     KKDIQTLHGKIGIKIPAGTSSGQALRLKGLGLPDKSGKFGDLNVKIKIVIPKNISQKQ
                     IDLYKKLADLD"
     gene            13300..13563
                     /locus_tag="PKI94_04255"
     CDS             13300..13563
                     /locus_tag="PKI94_04255"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNW25986.1"
                     /translation="MKKFCGKIIVKMKPDVKDIKGEALKQAVKSVVELHDLQCAVGNW
                     YQISFCAKNQVEALKFIDKIAQDILSNEVIETYEIKSLEEIDE"
     gene            13556..14158
                     /gene="purQ"
                     /locus_tag="PKI94_04260"
     CDS             13556..14158
                     /gene="purQ"
                     /locus_tag="PKI94_04260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNW25987.1"
                     /translation="MSKLKVGIIVFLGTNCEYDTKRACDFLGWNSEFVWQNDKIQKKY
                     DLIILPGGFSYGDYISSGRIAKLSPAVKSLPIGKTMILGICNGFQTLCESKLLPGALI
                     DNKNIDFISKVTEINFQNELISLPLAHHQGNYTCKNIDKSQILMTYQNNENGSDFAIA
                     GIYDKKNKIIGMMPHPERAMYEELGLTDGRKVFKFIQNEI"
     gene            14148..15719
                     /gene="purL"
                     /locus_tag="PKI94_04265"
     CDS             14148..15719
                     /gene="purL"
                     /locus_tag="PKI94_04265"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNW25988.1"
                     /translation="MKYKKFKLTENEYNHIKSELKREPNDFELHLFSATYSEHCSYKH
                     SKELLKLLPRENAVFPFENAGGIKVGDHVILFKMESHNHPCAVEPFNGSATGIGGIIR
                     DVLAMNARPIALCNSLKFGDKSKRVIDGVVKGISVYGNSIGVPNISTEVLFDDCYEQN
                     PLVNVTTIGITKADRVLTSNMAYAGLKLMLVGSATMKDGMGGAAFASKKLSDEKKSDK
                     LSIQIANPFAKKKLIEACLDIFSKKLVIACQDCGAAGILSSTSEVAYKSGCGVEINLD
                     KIHLGDKSIKDFEIMLSETQERMLFVVDEQNISEIENILDKYELEHSIFGKVIKEKKY
                     IVKNKGKIKADLPTVLLVNPPKVKIKFDNKKMKTQHKRTYSPTHLFTYSPIYEQYDHT
                     VGARTQFQPYENPQFNALWIYEENCSLGVLTFSVVIKNNPFEEVYEAILTASKSLEEK
                     SYKPIGITDCLNFANPEIPPTMHEFRQTILAIKKACEELNIPVCSGNVSFYNESNGSK
                     IKNTPAFTMLGIKRL"
     gene            complement(15714..17450)
                     /locus_tag="PKI94_04270"
     CDS             complement(15714..17450)
                     /locus_tag="PKI94_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNW25989.1"
                     /translation="MQISPINYKFNFNNRYFPNRHTAIVQQQTSFSVVQSSNIFYLPV
                     FEGKKREAKRLVRNEKKGSSEWFENRIVKQVKGKKYQGAGLYDKSGMYVDFLKIGNEK
                     LFQEPLDITKATDKEVYAYWHALALAETYDSSWVGRYNKFNITKPLAVFHTLNSEASQ
                     ELFAQNLRILHNPVRCKSLDTNITDEQGNLCINCSVFDTETTGTKTDEDNLSDNDRIV
                     QIGAVQIKNGKISKKSEYNQLINPEIPIPEEATAVHGITDADVENAEKLEAVLSKFLD
                     KYLNKENGVIVAYNSKFDIKLLNNAIREHNTHSDKRLKERPTFKVLDPFILIQRIHPY
                     LGVKKKLSGQYQFLFSKNLEEAHDALADVKGTVDVLKYCLYYLNEHRRDKSVPLTLRE
                     VLIFQNGGRVKNIDLPMDVENCNAAVNFNKSYLLASMNVDNYFKGYKLSKEIVDSIKD
                     EIGDVNIQKLVETGIINEKNILTHKGFKINPAETKRIPKSAKYENSFYILKSNFKEVL
                     GFADLEPYNGKTKDEIEDLIIEKSKLFLHKQSMPIWMKNPNPMDIKDGNDLPDLKIAR
                     KVMAENKEAENL"
     gene            complement(17455..19479)
                     /locus_tag="PKI94_04275"
     CDS             complement(17455..19479)
                     /locus_tag="PKI94_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNW25990.1"
                     /translation="MKIGVTTLAVSCTGHSFYARKKNNSTDTPVNSYGSYFNYNLSSL
                     AFCANDKRTLTTEKSFINPIKPQNAGKLTTISDITQSMSDYINIEAYKSGLTNDAFLR
                     KKLKGLHVLQVEPVESKTAIVGNTRVQTLIDGEQIFGKTLDYLKSAQESILIEMFEFQ
                     NLTVDGNKWAQNGAERVGGAREQQQILWTLIKKRQANPNMKIQVILDAHKWYIDGNGN
                     RVRHYANQDMIKFLKKNGIDVVPYPRGAQQGTLIQHIKLLVVDGKKAVLGGMNWGSHS
                     PANHDACVAIETLPKKKNSEVDNIIEEHFNRDWKFAWQRLGETKFVAGPLTQKEQKYY
                     HGIDKRIKQENVDYEKLLGEFYNTPEAKTRYKKGTLDLVHTNPVDDPQMEILGTKPKE
                     FELIGDEGTESTRRYLENKLLKCKKLRAELFVFTDYELIEIIIDRVKKGELDAQIIVS
                     SDILESFPPCRKGYIELKKNNIPVRLYKTDKRIRQRLHSKWAVFDDNEVLIGSTNWSQ
                     VALNQNLETGRRYDYDRSSKKIDDEIKQYFESVKGAENDLNLPHLSWNGSVISYEALK
                     QRKRLIRGAINRVTKDGSAQIEINKTKLSFSKKDLPKMQKVHAYYTIIQERHNAKVKY
                     KRGNNELTIVFKSPSLAKSVFEKQFDRDWKHSESEYEKLSSKVFEPKGKN"
     gene            complement(19611..20345)
                     /locus_tag="PKI94_04280"
     CDS             complement(19611..20345)
                     /locus_tag="PKI94_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW25991.1"
                     /translation="MSNTKQLFKNKNTTDDYSDFTKKSGQQIKVIIVEDFKLTRVGLR
                     CALNSNPDISVIAECDNAKSALDLIEKHKPDVVLMDLGLPEMNGIEATIRVKEMLPNT
                     KIVALTSHDREEEVIAALASGAHGYCLKDIDPIKLADVVRDVSSGVCWIDPQVAQLVL
                     NVFPKPENTSIFPSKADSGTRVPLTEREFEVLKHLVSGKSNTEIAKELIVSVHTAKAH
                     VCSILQKMCVNDRVQAAVKAVREGLV"
     gene            20655..21059
                     /locus_tag="PKI94_04285"
     CDS             20655..21059
                     /locus_tag="PKI94_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNW25992.1"
                     /translation="MTGSVIKISEAASLGLHAMIILAQNPKKLVSVKEISDLLEVSAN
                     HLSKVMQRLTKIGFINSVKGYNGGFKLIKAPEKITFLEIYELFDGKLPTSSCLLTTHK
                     CKGNCILGDLVTSVNKQVKEKFEKTKLSDFVK"
     gene            21116..21922
                     /locus_tag="PKI94_04290"
     CDS             21116..21922
                     /locus_tag="PKI94_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW25993.1"
                     /translation="MKRDIIKIDEKKCNGCGNCIPGCPEGALQIIDGKARLVSDLFCD
                     GLGACIGNCPAGAMKIEQREAEAYDEYKAMKNIIKGGENVIKAHLKHLDEHGELGYLS
                     QAIDFLEQKNIPVPDYKGRTQCNCPSTMSKKIDRENFPEKTINLTPQLNNWPIQLALM
                     NPDAQYFENADLLISADCVPFSYPNFHQKFLKDKVLIMFCPKLDKTLEQYIEKLTYIF
                     KNKNIQSVSIVHMEVPCCSGVEIIIKKALEKAQKIITIKDYTISISGEIV"
     gene            21936..22103
                     /locus_tag="PKI94_04295"
     CDS             21936..22103
                     /locus_tag="PKI94_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25994.1"
                     /translation="MSFYQYEQTAKRENCRLARACQKHLQSVYLQNKIIAKIKELEFY
                     ASKQKNITKLS"
     gene            22481..23506
                     /locus_tag="PKI94_04300"
     CDS             22481..23506
                     /locus_tag="PKI94_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25995.1"
                     /translation="MMQVPTVQPQQAYAQQPSYNAVKIDVHNPQVNAPGATPAQVPSY
                     VTAPVYNVPNASVYEVPQQTIYGPQQNFNQPPVAQQPSVPAPVVVTPQPQTPVAPAAA
                     PQQPEVKAPEAAAPQVDLNAFLGKLGGENLDEQANTMEQIAEITQNNPQKASELLDTR
                     IVDALLAIMQKDTSKLQGPTQKQLEIREKMMKNETVTDAEKAEAEAITPMEKAETNKQ
                     YAMYTTAMLQNLYISEIQKMNNTVVPMTELPGAAGIVEQVKNNPNPIVRASGVDSLSY
                     IQRPEYKQDLTTIFTIAQKDQDKLVQEAATKALEKINQVAAAPAATPATEAPKAEAPK
                     AEAPKAA"
     gene            complement(23564..23836)
                     /locus_tag="PKI94_04305"
     CDS             complement(23564..23836)
                     /locus_tag="PKI94_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25996.1"
                     /translation="MEKLYDILLTILFTACLVLTVPVWACSDYDGITGGACSIIDLNK
                     DYMSKKQKEESENFDLRPLKNKKEQKMTSVELICKHSACLLRFHMK"
     gene            complement(23977..25116)
                     /locus_tag="PKI94_04310"
     CDS             complement(23977..25116)
                     /locus_tag="PKI94_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW25997.1"
                     /translation="MKKILIFLAAFLMFQTACFAQTGITFLYINGSNNNDAKMKNWYE
                     RGVKKLHPHMKREFESNKLTQEYFLKNGEYFIEQQPAIFFWGDKSHRDLAFVESDLNI
                     SKTFSPWIAYQIRFALTRFLHDAIWVQKYHNMLPVVEDLHKMVVSENEKGNKVVLYGY
                     SAGSFVTYEYMLTRLPFIETVDFFNNVDVTPAQREFVAKHPMKNTCMRALGEDLAVFS
                     AAGHIVPNKNDSLFKEKYMNLNNLTDQVCTPDNSLKGVVNFASPLVLFYSDISDPNYQ
                     ITYYNRLLLEYLIENDMFWLTVNYREDPLGFPCGRNLSIEEMEEIAKLDIEPHAGFVY
                     DKSNTRGTRGVFMAHTSYWSTGKVFSKAVVNAYVNGYTNRKKKAE"
     gene            complement(25138..>26219)
                     /locus_tag="PKI94_04315"
     CDS             complement(25138..>26219)
                     /locus_tag="PKI94_04315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNW25998.1"
                     /translation="FLLNSKNINILKGKGTILSKNIIKVVADSGEFLVSFDKLIITSG
                     SEPLKPNIKGIDLKNVITSNEAINIQEIPQNLTIIGAGVIGLEFATFFNALGSKVSIV
                     ELQQEILPHEDKEAVEELIKIMKRRGIKFYLGAGVNEIKEVDSELVTIFEQNGQNINL
                     NSNYVLVAVGRKLNLSDDILNLGIKTKNGAIEVNEFMQTNVEGVFAAGDVTGGMLLAH
                     LAFAQGKIAAENACGHKNKFNEVVPSCIYTHPELASIGLVEHEAGGRKLKIGKFEFRH
                     NGRSLCNGHREGFVKVLVDASSDEIVGAHILGANASELISELTLAIKLKAKAQDLANM
                     IHPHPTLSEAVMEACADAAGYPFHK"
BASE COUNT         8898 a         4862 c         4221 g         8238 t
ORIGIN      
        1 tcaagaaaga ctggaaattg aaggttcatt tccaagaata atagaaggta tatgccaagg
       61 atttagcaat ttaaagtcat attatcaagg cttaaataca caatgtgaag gaaacgaagt
      121 aaatattgat tctatttccg aaaatttcac aaattttgat gctttaattg ctcaattaat
      181 cacaaaaatg caagacttta acgaagctat ggcagttgca aacgaagcac atcaaactgt
      241 tcgtgagcgt gtagaagaat acggaatagc agcgtatctt gcatatcaag cctatcctga
      301 tgatcaaagc taccaagacg aagcaaacac tgcttatgca gcctggatgg cagcaggaac
      361 tgccgctatg gctgccgatg gtaatttaga taacattaaa aatgattttt tcggcatcat
      421 aacagacggt ctcacacctt taataaataa tataaacgca tcatttatcc aagctacaca
      481 accaaaacca cctacagagc ctccgacaga ccctgataat acatatacat acgatgcagg
      541 tagagtattt tactacagta atattttcaa cgagatgcta aaaggttata taagcgttga
      601 agacgaaaag ttcaatgatg caacctggat tcatgatcaa attaaaaatg gaaacattca
      661 tatggaagaa tattctgaaa gtgcaaatgg cggtcagggt ggatttgtag aaacatccat
      721 cgcccaagat tcatcattat atgaagaatc ttatgaaaaa aataatgccg cggcacaagc
      781 agaatatgat cgtttaatat ctgacttaaa ctataaagaa aaacaattag acatgaaact
      841 taacaccata aatactgaat ataatgcagc aaaagaagaa tatgaatcag tcaaaaagac
      901 tcaagaaaag agtattgaca gaacttttaa aatatttggt taattgctac tttcttaaat
      961 attaagggat tgtaaagaaa ttttacaatc ccttttaaaa ttttaaagtt caaataaaca
     1021 aatgccgata aataagacat agggaaaaac gttacaaaga aatcaaaaat tcaaggagaa
     1081 actaccaatg ggtatggcag catcacaagc aaggctcttg tctttaaccg caaggttatc
     1141 ggatttagaa tttcaagcta tgcaacttac aaatgcaaaa attcagcttt ccaggtcaac
     1201 tgaagcggtt gcaagcgaat acaccaatgc ccttaacaat aagattttta aaataaaaaa
     1261 tacaaatacg cctttaacgg caagctcatt aacaggattc caaacacttc cttcaataga
     1321 agctcaaagg attcttgttg atacatcaga tagaatatta gtctccggta ccgttgcaag
     1381 agcattcgat actgccggag gcagctgtga aagattttta aaccaattcg gatatacaag
     1441 ccttcataat cctccaaata ttggcggtga ttctgatcca acaggaggca acaataccac
     1501 agaaataata aaaggggtta gtgaatctac cacaaactca agtactgaaa cagatgtgta
     1561 tgatatctta tcaatgcccg caacttctgc ggcaaataac aacgataaca actccggatc
     1621 aggagtaaac cctggcaaca actggggaac aagcagcggc aatacttggg gaacaggcga
     1681 cggcaatact ccttcaactc ctcctcctcc gccaactgta gatcctcctc cgccaactgt
     1741 agatcctcct ccgccaactg tagattcccg ttatacttac gatgctgatg cagttcaata
     1801 ttatactaat ttgttcaatc aaatccaatc tcacggatat gtaacaactg acgatcaaaa
     1861 tttaagcaat cctgaatggc tatatgaaca attattcaac ggcaatttga gaatggaaaa
     1921 attaacatca acaggtcatg cccaagaaga tcaatggaat acagatggct tacttgaaga
     1981 agtttatgat acttctgatg atgcaagagc agaactcaaa tacgaagtag aaacaactaa
     2041 aatacaaaat caggacaaaa aatatgattt agaactaaaa caaatagaaa cagagcatac
     2101 agctattcaa acagaaatgg aatctgttaa aaaagttatt gataaaaata ttgaagcaac
     2161 attcaaaata ttcgcgtaaa ccgaatattt aaaaagaatt aatcctaccc attccctaaa
     2221 tttcgtaacg tttaatgcgc caactgaaaa gttggctttt ttttgtgcta cgcacgtagc
     2281 cactctgtat agattaatac agtgcaaatc gaccaatgtt ttcgggcttt ttataaacat
     2341 caacgtcgtt ttgtttttca ttaagaaaac tatagattca gattcataaa cttttatcaa
     2401 catttttgat aaatatattt ttatttgtta tatctttagt taaaataaaa aaaagaaaga
     2461 gagacttaaa tggaaagatt tttcggatta ataggaattt tagtaatatt cttaatagca
     2521 tttttaatgt caaacaacaa gaaacttatc aatataaaga ccgtgggaat ggggtttttg
     2581 ctccaaatat tacttgcggt atttgtttta aaaacacctg ttgggcaagc aattttttat
     2641 gatatagggc tttttatcca aaagatactt caatttgcaa atcagggtgc atattttctt
     2701 ttcggaccgt taagtgataa catcaaactc ggaaacgttt ttggtaaagg ctctatcgtt
     2761 tttgcggttc aactgcttgc aacgactata tttgtgagtg ttatagttaa cattttatac
     2821 tattacaaaa ttatgcagcg cgtgattgca atcttaagca aaggcatgta caaattaatg
     2881 aatgtaagcg gtgctgaatc cttctccaat gtggcaagtg cttttgtggg acaaatcatg
     2941 gcacaaatta tgattaaacc ttatctgcct aatttaaccc gctcagaact tcttgcatct
     3001 atgactggaa gtatggcatg tatttcaggt gcggttatgg caatttatat aggaatggga
     3061 atccccgcac agtatttgct tgccgcgtca attatggcag cacccggtgc tttagtaatt
     3121 tcaaaaattg tctatcctga agacggaaca ccgcaaactc aaggcgaagt taaaataagc
     3181 aaaagaagaa cccatgtcaa cgtcgtagat tcaatcgcaa gaggcgcatc agatggtttt
     3241 tccgttgctt taaaagtttt tgcggtattg attgcttttg tagctctaat tgcaatgatt
     3301 gactggcttt taggtaaaac cggggtattt ttgcacaacg cattacatat aaatctttcc
     3361 tttatcgggc ttgatatcga taacctttca atgaaagcta tagtaggcaa actttttgga
     3421 ctttttgcat atgtaatggg agttccgctt aaagattcaa gtattgtagg ctcattaatg
     3481 ggcacaaaat ttatcttaaa cgaaatgctc ggttatatcg atttaatttc ggttaaaaat
     3541 ttaattgcgg acaaaagctt tataatagca agctttgccc tttgcgggtt tgcaaatatc
     3601 agctctattg ctatacaaat cggaggtatc ggagaaattg cgcctaatca aaggaaaaat
     3661 atttcacgat tggggcttcg cgcgctaatc tgcggaactt tagcctctta catgtccgcc
     3721 tgtatcgcgg gaatattgct tggctgattt aagttataca aaaatctaag cccttcaagg
     3781 gttagggtgg ggttaataaa gtcaaaaggg cgtttcatat agcttgaaat aagccctgaa
     3841 taaccgcctg ttgcaataat tacagctttt tgcccgagtt ctttttcgca ctgttcgaca
     3901 agtccgtcaa tcatagctgc agaacctctt ataacacccg acagaattgc atcttgtgta
     3961 gtttttccta tcgcacaatt tgaaatcgcc acctcgattt tagggagttt tgaagtgaat
     4021 ttgttcaaaa cattcatctg caaattaagc ccgggtgcaa taattcctcc tacaaattcg
     4081 ccttttttat tgacaatgtc aaaggaagtt gcagtcccga aatcaaccac aatacaaggt
     4141 tttttgtaga gcataaaggc cgcacacgca tttgcaatcc tatctgcacc ggcttcttgc
     4201 ggagtgtcgg aaacaatttt aacacccatg ttaatctcgt gagttaaaaa catcggacta
     4261 atgcccaaaa cattttcaag ggcaaatttg aactttgaat ttaactcttc aacgaccgaa
     4321 gctataatgc aaccttctat tttaaaagat ttaagcaaag ttttaagtaa aacctcatat
     4381 tcagcttgcg acaaatccct gtccgaagcc aatctaaact cttctacaag agcatctcca
     4441 taaaaaatgc ccaatgtaat gcttgtgttg ccgatatcaa cagttaataa cataaaatgg
     4501 tcctatcgtt tgatagaacc attttataat aaatattctt ttatttaaag actatgccat
     4561 aaaccctgtt cctgtagttt gaggcggctg accttgtcca aaaatactcg gactttgagc
     4621 atacgctgtt ttgtcctttt taggttcttc tttttcttcc aagcccttaa ataccggtgc
     4681 ttttgcacca attttgtcaa ctgcaaatcc cataattata tcctcccttg ttatgtaata
     4741 atttttcctt tatatccctt atctatataa agtatttttt aaagagaaaa tttactcaat
     4801 taaaggataa tgttacaaag tttacaaacc tttttctaca agctcaattt cataaccgtc
     4861 agggtctttt acaaaagcga ttaaagattt actgtttgga atgttaaagg gttcatacaa
     4921 atattccaag cccatttttt taagcttttt gtcgaattca ttcatggaat caacctcgaa
     4981 agcaaaatgc ccgaaacctg agcctacttg atagccttct tcaggagttt cgtcgttaaa
     5041 agtcaattct atttgagctt cgccatgctt atccgaaaga aaaaacaaat tacaatcacc
     5101 aagccgcatt tcttttacca attcaagctc aagaagttca ctgtaaaatt taagcgattt
     5161 ttctatatct ttaaccctaa tcattgtatg tagaaatttc ataatgcctc ctattatttt
     5221 gatatattga taaaactcgt agttgcgaac cctttagggt gaagcaatcc aaccatttca
     5281 tataattaga ttgcttcgtc gcgttgctcc tcgcaatgac aaaattattt agactttgtt
     5341 aataaaacaa cagcatttgc ttcaatagcc tctaatctac ccacagcatc cattttttcg
     5401 ttggttttag ctttgacgga aattaaatca aaatcaacac ctaaaacatc ggcaagattt
     5461 ttcctcattt gaggaatgta aggcatcatt ttaggttctt gagcaataat atttgaatcg
     5521 atattgttta tttcgtaacc tttttttaaa attaactcat agactttttt gagcaaaatc
     5581 agacttgaaa tgtttttaaa ttcatctgcg gtatcaggga aaagagttcc tatatcagaa
     5641 agcgcaagtg ccccaagtat tgcatcaatt atggcatgga ttagaacatc ggcatcagaa
     5701 tgcccaaata accctttttc ataaggaatt ttaactccac ctataattaa agcccgatta
     5761 tcaactaatt gatgaatgtc ataacctgtt ccgattctaa acttttgcat tgtaagcatc
     5821 cacaaacctt tctaccgctt tgacaaagcc gttattgtta acagtgtcag ttatatattc
     5881 agcaacagct tttaattccg gcgtcgcatt tcccatcgcc actttaagtc ctcctgctgc
     5941 aagaagttca atgtcattat tttgatcacc tatggtcata acttcttctt ttttgaatcc
     6001 gtagtgcttt cttaaaaatt ctaccgcaca gaattttgta gcttcgccgt gacaaacttc
     6061 gcaaaaatac ggtgttgact taaccacatg aagctgagga tagtttttcg aaaggtaatt
     6121 tgcccaatct gtaactcttt gatgattgtt aaaatcaatc aataaaagtt tgtttaaatc
     6181 atgcaaatca agtccttcaa aagattttac aataaaacct gttgaacgct cgcctgtata
     6241 tcttctgaca atttcatcgt ctttttcaac gtaaagttca tcgttcatat acagatttac
     6301 gtgaacatca ttttgctttg cccagtttaa aatttctctt gtttccgtaa taggcaaagt
     6361 cctttgataa agaatttcgt cattgtgttt aaccattcca ccctgataag aaattatagg
     6421 agtatcaagt ccgagttctt ctgcgatata atctgttgcc gcatgcattc tgcccgtaac
     6481 caatacaact ttaatccctg cttggcaaag tcctttaatg cagtctttaa cttcctgatt
     6541 aaactcaaaa ttgtatttta aaattgttcc gtcaatatcg gttgctataa gtttaatatc
     6601 tgccataatt tcctttaagg taattttata aatcctttta aacctgcgcg cataatggca
     6661 caaatagttt tcgcatcgtt tatttcgcct gagttaatca tatcaaaaac cttacccaaa
     6721 ttatactcga aatattcaat aatttcacca tcatcggggc tatgtttttt atattccaag
     6781 tcgcgggcaa gataaaagtg cagtgtctca tcacaaaatc cggggcttga ataagcaata
     6841 cccaacttat cccatttttt tgcaacaaag cccgtttctt cttcaaattc tctttttgct
     6901 gctaaatcag gattctcgac cctgtcgagt tttcctgcgg gaagctcaag agaaaccaat
     6961 ttaagcggat atctatactg ttttaccaaa agtattgtat tttcatcttt cagtgctaaa
     7021 atcacaacgc cgcccgggtg gtttaccact tcccttattg tcttttcacc ggttgaaagc
     7081 tcaacatcat cctttttaac atcaaaaaca attcccttaa aaatatgact tgattttaat
     7141 gttttttcgt aaaatttttg cataatcccc tcaaaaaagt gcttttataa tgctttttat
     7201 atttccatca gttatttttg acaagacatt ctcaaagtta atttcttcaa gcttgcctaa
     7261 tgatttaata acttcaacgg tttttagaat tatggtttga ttaggcgaat ttaaaagaac
     7321 cttcagctta tcaaaagctt ctgtcatgcc tagttcttga attaaatcaa gcgcaggaaa
     7381 aacaaaatca ctcaattggt caagttcatt aagtgccaaa tctttttgaa tattccaaaa
     7441 aagctcaaat tgtgaattta aaagcgtttt gatttcaaaa acttcatttt tagtatcttt
     7501 atcttcgtca aaaatatatt catcattttc tgtcaattgt tcgaatttaa gttttgcatt
     7561 caaaagcact atggcgattt tagggctttt ttcttccttt tgtagttcaa taagtctttc
     7621 caaaacatcg taaagttgaa aatcaaaaat ctgggaaagc gaaacaattt cgccaagccc
     7681 gttgattata tgattaacag ccaaaatagt atcatatttt aaatccgtat caaaaaattc
     7741 taaaaaactt tgcagatatg aaatttcaga agcaatgttt tcaggcattg aagatttttt
     7801 catggtttta aaaatatttt ctatagcacc tgtatcgtta taagagatta aaaatctgac
     7861 cgcagaaatt ttttcaaatt cgtcatcgga ctcaagtttt tgtagggcaa gattataaga
     7921 aaaatcatct ttcattttag aaagtgcggt cgcgcaattc atcgcaagag ggtcaaaatc
     7981 agagtaagaa tattttctta ataaatctat tgccaaagta tcattaataa aggaaaaata
     8041 ctttgcacag taagcttttt gagcatttga accacactct aacatttcaa gcattttatc
     8101 ggttaaatct tcatctgcga atttggtaag cccatttata ataacctctt cataatcaga
     8161 cgaataaacc ttcaaaaaat tcaaaagatt aaggtaatta tttttattta cggcattttc
     8221 aagccttttt gcaacattgt gtttaataaa atcaaataaa aaatcgcttt tttctaccaa
     8281 aacagaaaaa gcttttacat cagaattatc aattatatga gccgcggcat actcaaaatc
     8341 tttagggttt ttgcccgtaa gctttttgac aatatcttca gacataagct agtccaaata
     8401 gaaaacggta actactttat gagcctcttc ggcatattcc acggctttgt ggagggtttt
     8461 gcttgtatgt gaaagaaaac aaataagctg attgcaatca tcaataattt ctctgttaca
     8521 tacccttgaa gcatctgcaa gtgtcatcat tgctctatcc ggatgctcaa taatatttgg
     8581 aacaccaata agctgatctt gaacatcaga aggctgctga ccaattgttt ggggcaaaat
     8641 aacttttaat ttatcgggat ttgccttcat ggcacctctt atagcagctg cgtttgttcc
     8701 ggaagaaccg cctgatgtaa ctattgtgct gccctgcata acaagtgcca ttgccaaagt
     8761 ttcaattaat tgttggtgcg taataggtaa atttcgactt ccgattattg caaaacgttt
     8821 tgcagattgc tgaattgtag ccaattccat tgcaagttcg tcaacagtat cgggcgaaga
     8881 atctgaaaca atgtccaaat catcaaccgg aaccataata gacggttgtt cattttcggg
     8941 ttttccatct gagttcaaaa ttttttcttc taccattttt ctaccttctt tacacagtca
     9001 actttaaaaa aaactttttt ttagtatgat aattgtagca caaaaattaa atttgaaaag
     9061 agggggtata agctgaatag gtgaacagct gaaaaggtga atagttagat ttttataaat
     9121 gcacttattc acatattcac ctatttactt attcacctat taaaaagaag agaaatgaaa
     9181 gatattttgg ataatttaaa taaagaacag attgaagctg taaagaccgt taaaggtcct
     9241 ttgcttgtac tggcaggtgc tggcagcggg aaaacaaggg tattgacttc aagaatagca
     9301 tatctcatca agtccggagt gcgcgccaaa gaaattctgg cggtaacttt taccaacaaa
     9361 gctgcaaaag aaatgcgcga aagacttgct tcaatgctcg gcgatgaaat cgtaaaatat
     9421 atgtgggcag gtacttttca cagtatttgc ggaagaattt tgcgccaaga cattgaaaac
     9481 tacaaatttc aatcagggaa attcttagat aaaaatttta ccatttatga tgaacaggat
     9541 tcgcttgcgg taataaaaca ggcagtcaaa aagcttaacc ttgacgacaa aatttacgca
     9601 ccgaaactta taaaagcaat tatttcagat tcaaaaaaca aaatgcaaga tgcttattct
     9661 ttttcgactt ttgcaagaga tttcaaatca caaaaaattg ccactgtttt tgaggaatac
     9721 gaaaatatcc tgaataacaa taatgcaatg gattttgacg atatgctcat gcttgcggta
     9781 aaacttcttg aacaaaatcc acaaattcgc gaaaaatatt tttcaagatt cagccatatt
     9841 ctggtagatg aatttcagga tacaaaccag gcacaataca ggcttgttaa tatgctctac
     9901 acaaatcttt ccgacaaaca catggaaaac cgctcgcttt gcgtcgtagg cgacattgac
     9961 caatccattt acagttggcg cggtgctgat tttaaaattc tgcttaattt tcagcgtgat
    10021 ttcagaaata caaagatgat aaaacttgaa caaaattacc gctcaacaga aaatattctg
    10081 aatgtggcaa atgcaattat tgaaaacaat gaagaacgtg ttgaaaaagt tctttattcc
    10141 caaaaaggcg gaggtgaaaa aattcaatat tttgaagctc aggacgaatc cgacgaagcc
    10201 aattttattg ctcaaaccat taaagaaact tcccctgatt ttaatcaatt tgccgtactt
    10261 tacaggacaa atgcccaatc aagagcactt gaagaagcat gtattacata cgggcttcca
    10321 tacaggattt acggcggact taaattctat gacagaaaag agattaaaga cataattgca
    10381 tatttgaact taattcacaa taccgatgat tcgattagct ttaaaagaat agtcaatgta
    10441 ccaaagcgcg ctatcggcga cactacaata aaaaatcttc aaatgatagc agaccaaaaa
    10501 gacataagct tatttgaagc ggttttacag cttgatgaag tcgaaggaat taattcaaaa
    10561 acaaaagaaa aattgaaaaa attcacagac ttaattctag attttaaaaa agttcagcca
    10621 aaatataatt taagagaatt tgtatcacat gtaattgaaa aaagcggata tttaagagaa
    10681 ttgcaggaag aaagaacccc cgaaagcgaa tcaagaatag aaaacttgca ggaattggta
    10741 aatgtagccg gtgaatttga gcccgaagat attaataacg cttacagcga atttcttgca
    10801 caaattgcac ttgtatcaga tattgacagc cttgataaca tatcaaataa cgttacttta
    10861 atgactttac actctgctaa agggcttgaa tttccgacag tttttgtcgc ggggcttgag
    10921 gaaggaattt ttccgcatca acgcaccttt aattcacctt ccgaacttga agaagaaaga
    10981 cggcttatgt atgtgggcgt tacaagggca gaagaaaaac tttatttgac aagcgcaaaa
    11041 cgcaggcaaa tgtggggtga atttaaatat tacaacccga gccgtttttt ggaagaaatt
    11101 cctcaagagt tacttgaaag ctctatttca caaaattacg aaactcaggc tacatttaca
    11161 agtgccgcaa ataaaatcaa aggggtaaat gtaggggtaa aagattataa ggcaccgaag
    11221 gcgcagcctt cggtgccctc tgatcgtttc ggctacattc cgccgtcaag cggtttcggg
    11281 aaaaactttg tagcaccgca aaaacgaaaa aataatgaca tttcaagcaa atcaattctt
    11341 gtaaaatcat cgataaacaa agaaaaagaa caagaaaaaa tcgacaaatt tttcaaggac
    11401 aacgccataa aacggatgct tgccgaaaag aaaaaagaag aagcaaagaa aaacgctgaa
    11461 gcacaaaaaa agtttaactc cgtcccttac ttttttaatg aaggcgaaag agtatttcac
    11521 gaaaaactcg gaattggaca cattgaagca gtaacccaaa taaacgaaag cacaatgtac
    11581 accatagatt ttggtgataa ggggaaaaaa gctatggatg cggcttatgc taatttaaaa
    11641 aagttttagc ttaaattgat gcttaattca aactaaaccc atgttaagtg tgttttttgc
    11701 ttatgaaaat attcgtcttc ttgttcataa attgttttta acgcaagcgg gtcaaatatc
    11761 ccgctatcag gagttttaat gacattttta gtttgattta attccggatc ataaattgaa
    11821 acttcaaaga catcctttgg agcaattcct gttccttcaa actcatcggg taaagaagat
    11881 tttgccttaa aaatatttac gacgtacttg tcaccattat gcctcattgc atggataacc
    11941 tcattccaag cacctttgtc agaaggattt tgtctagcat attttaaaaa ttcagaattc
    12001 ataaaaacat gcgaagtaaa attaactttg ttttgaaaat tagaaatacc attaatttgc
    12061 ataaatttct cctatattta ttatgataca ttaaaaagta tgataaaata atttgctagt
    12121 atattacaaa aaattaacaa ataatttaaa aattaatcta aatccgcaag ttttttatac
    12181 aaatctattt gtttttgtga aatatttttt gggataacaa ttttaatttt gacatttaaa
    12241 tcaccaaatt ttccggattt atcgggcaaa cctaaacctt ttagcctcag ggcttgtcct
    12301 gaagaagtcc ctgcaggaat ttttataccg attttcccat gcaaagtctg gatatctttt
    12361 ttgcagccca aaaccgcttg agaaggcgtg atttcaattt ctttggcaag atttatccct
    12421 tcaacctgat actcattgtc tttaatattt atttttaaat ataaatcgcc ttttctgccg
    12481 taggcatctg ctttaccttc gcctttaagt cttatttttt gaccgtctgt gacttcagca
    12541 ggcaatttta cattcacacc tttttgttct gaaataattc ctgtcccccc acaagaaggg
    12601 caaaagccac ctcttggagg gcaatgcgga catttttcca taattttaaa acgcacaggc
    12661 aaaggttttc tgttatacaa atcttttgcc gttattgtta aattttgcgt tatatctaaa
    12721 ttttgagcct ttggagattg acgactttgc gcttgttgag aaaaacccaa atcatcataa
    12781 atatttctgc ctcttgaaga ataagaagac tgccttgcct gtcccgacat catatcaccg
    12841 aaaagcgaac tgaaaaagtc ggaaaacccg cccatttcat caaaaccgaa agtttgactt
    12901 tgccttccgc cctgaccaaa attaaaatta aattgttcaa atccgggcgg cggagtaaag
    12961 ttggcacctt cctgccaatt tgcacccaaa ctgtcgtatc gttggcgttt ttctttatcg
    13021 gataaaactt cgtaagcttc gtttatgtct ttgaattttt gctctgcttc ttttgattta
    13081 ttcacatcag ggtgaaattt tcgcgcaagt ttgcgataag ccgatttaac ttccgcctct
    13141 gttgcgcctc tttttacacc caaaatttca taataatctt tgtactgcat aatactatga
    13201 taatacaaaa taaactaact ttgggcataa ttaattattt tttaataagt gcctcatctg
    13261 acaatagagc aaaattaagt ttcatgataa aattgtttta tgaaaaaatt ttgcggaaaa
    13321 atcattgtaa aaatgaaacc tgatgtcaaa gacatcaaag gcgaagctct taagcaagct
    13381 gtaaaatccg tcgttgagct tcatgatctt caatgcgccg taggaaactg gtatcaaata
    13441 agtttttgtg ctaaaaatca ggttgaagca cttaaattca tagataaaat tgctcaagat
    13501 attttatcga acgaagttat tgaaacttat gaaatcaaat ctttagaaga aatagatgag
    13561 taaacttaaa gtcggaataa ttgttttttt ggggacaaac tgcgagtatg atacaaaacg
    13621 cgcttgcgat tttttaggct ggaatagcga atttgtttgg caaaatgata aaattcaaaa
    13681 aaaatatgat ttaataattc ttccaggagg attttcatac ggagattaca tttcatctgg
    13741 cagaattgca aaattatcgc ccgcagtaaa atcacttccc ataggaaaaa ctatgatttt
    13801 aggaatttgc aacgggtttc aaaccctgtg tgaatcaaaa cttttgccag gtgccttaat
    13861 tgataataaa aatattgatt ttatttcaaa agtcactgaa attaattttc aaaatgaatt
    13921 aatttcactt cccttagcgc atcatcaggg gaattacacc tgcaaaaaca tcgataaaag
    13981 ccaaatttta atgacttatc aaaataacga aaacgggtct gattttgcta tcgcaggaat
    14041 ttatgacaaa aaaaacaaaa ttatcggtat gatgcctcac cctgagcgtg ctatgtatga
    14101 agaactcgga ctgacagacg gaagaaaggt ttttaaattc attcaaaatg aaatataaaa
    14161 agttcaaact gacagaaaat gaatacaatc acataaaatc ggaacttaaa agagagccga
    14221 acgactttga acttcatctt ttctcggcaa cttattccga acactgctct tataaacact
    14281 caaaagaact tttgaaactt ttgcccagag aaaatgccgt atttcccttc gaaaatgcag
    14341 gcggaattaa agtcggagac catgtaattt tatttaaaat ggaatcgcac aaccacccat
    14401 gtgcggtcga accttttaac ggatcggcaa ccggaatcgg cgggattata agagatgtcc
    14461 ttgccatgaa cgctcgcccc atagcacttt gcaactcctt aaaatttggg gataaatcaa
    14521 aaagagttat agacggggtg gtaaaaggaa tttcggttta tggaaattca atcggagttc
    14581 ctaatatttc taccgaagtt ctatttgatg actgttatga acaaaatcct cttgtaaacg
    14641 tcaccacaat aggaattacc aaagctgata gggttttgac ttcaaatatg gcatacgcgg
    14701 gcttaaaact tatgttggtg ggctctgcta ccatgaaaga cggaatggga ggagcagctt
    14761 ttgcatccaa aaaactttcc gatgaaaaaa aatccgataa gctttcaatt caaatcgcaa
    14821 atccgtttgc taaaaagaag cttattgaag catgtcttga tatcttctca aaaaaacttg
    14881 ttatcgcctg tcaggactgc ggcgcggccg ggattttatc atcaacaagc gaagtggctt
    14941 acaaaagcgg ctgcggagta gaaataaatt tagataaaat tcatctaggc gacaaatcga
    15001 taaaagattt tgaaataatg ctatcagaaa ctcaagaaag aatgcttttt gtggttgatg
    15061 aacaaaatat ttctgaaatt gagaatattt tggacaaata tgaactggaa cattcaatat
    15121 tcggcaaagt tataaaagaa aaaaaatata tagtaaaaaa taaaggtaaa attaaggcgg
    15181 atttaccaac agttttgttg gtaaatccgc caaaagtaaa aattaaattt gacaacaaaa
    15241 aaatgaaaac acaacataaa agaacctatt cacctactca cctattcacc tattcaccta
    15301 tatatgaaca atacgaccac acagtcggtg caagaaccca atttcagcct tacgaaaacc
    15361 ctcaattcaa tgctttgtgg atatatgaag aaaattgttc actcggcgtt ttaactttct
    15421 ctgtagtaat aaaaaataat ccgtttgaag aagtttatga agcgattttg actgcgtcaa
    15481 aatcgcttga agaaaaaagc tataaaccta taggaataac cgactgcctt aattttgcaa
    15541 atcctgaaat tcctcctaca atgcacgaat tcagacaaac aattcttgca atcaaaaaag
    15601 cttgtgagga attaaacatt cctgtatgct ccggtaatgt atccttttac aatgaatcaa
    15661 acggaagcaa aataaaaaac acacctgcat ttacaatgct tggaataaaa cgattataaa
    15721 ttttctgctt ctttattttc agccataact tttcttgcta tttttaagtc aggcaaatca
    15781 tttccatctt taatatccat tgggttgggg tttttcatcc aaataggcat gctttgctta
    15841 tgtaaaaata attttgattt ttcaataatc aaatcttcaa tttcatcttt tgttttgccg
    15901 ttataaggtt ccaggtcggc aaaacccaaa acttctttaa agttagattt caagatataa
    15961 aaagaatttt catatttagc ggattttgga attctttttg tttcagcggg attaatttta
    16021 aatcccttgt gtgttaaaat atttttctca ttgataatac ctgtttcaac caatttttga
    16081 atatttacat cgccaatttc gtctttaata ctatcgacaa tttctttaga taatttataa
    16141 cctttaaaat aattatcgac attcatcgaa gcaagaaggt acgatttatt aaaattaacc
    16201 gcagcattac aattttcaac atccataggc aaatcaatat ttttaaccct tccgccgttt
    16261 tggaatataa ggacttctct gagcgtcaga ggaacggatt tatctcttct gtgttcgttt
    16321 agataataaa gacaatattt aagcacatca accgtaccct taacatcagc tagcgcatca
    16381 tgggcctcct caaggttttt actgaataaa aattgatact gaccggacaa tttctttttt
    16441 acgcccaaat aagggtgaat tctctgaatc aatataaacg gatcaagaac cttaaaagtg
    16501 ggccgctctt ttaatctttt atcagaatgg gtattgtgtt ctctgattgc attattaagc
    16561 aattttatat caaatttcga gttatacgca acaataacac cattttcttt attcaaatat
    16621 ttatcaagaa attttgataa aacagcttca agtttttcag cattttcaac atcagcatct
    16681 gttataccat gaacggcagt agcttcttca gggataggta tttcaggatt aataagttga
    16741 ttatattcgg attttttgga tattttaccg tttttaattt ggacagcacc gatttgaact
    16801 attctgtcat tgtccgaaag attatcttca tccgtttttg taccggttgt ttctgtatca
    16861 aataccgaac aattaatgca aagattacct tgctcgtcgg taatattagt atcaagtgat
    16921 ttacaacgga cgggattgtg caaaattctt aaattttgtg caaaaagttc ctgtgaagct
    16981 tccgagttaa gagtatgaaa aacagccaaa ggttttgtta tattgaactt attgtacctt
    17041 ccaacccaac ttgaatcgta agtctctgca agtgcaagag catgccagta tgcataaact
    17101 tctttgtcag tcgcctttgt aatatcaagc ggttcttgaa aaagcttttc gttgccgatt
    17161 tttaaaaaat caacatacat accgcttttg tcatacaaac ctgcaccttg atatttttta
    17221 cctttaacct gtttgacaat tctattttca aaccattcgg aactgccttt cttttcgttt
    17281 ctgactaacc tttttgcctc tcttttttta ccttcaaaaa caggtaaata aaaaatgtta
    17341 gaagattgaa caacagaaaa agaagtttgc tgttggacaa ttgcagtatg cctattaggg
    17401 aaataccggt tattaaaatt aaatttataa tttataggac ttatctgcat aaagctaatt
    17461 ttttcctttc ggttcaaaaa ctttagagct taatttttca tactctgatt cggaatgttt
    17521 ccaatccctg tcaaactgtt tttcaaaaac gcttttagcc aaagaaggag acttaaacac
    17581 aatagtaagc tcattatttc ctcttttgta cttaactttt gcattatgac gctcttgaat
    17641 aattgtatag tacgcatgaa ctttttgcat ttttggaagg tcttttttac taaaagaaag
    17701 ttttgttttg ttaatttcga tttgtgcaga accgtctttc gtcactctat taattgcacc
    17761 cctaatcaaa cgctttcttt gtttcaaagc ttcatatgaa ataacgctgc cattccaaga
    17821 taaatgcggc agatttaggt cgttttcggc accttttacg gactcaaaat actgtttaat
    17881 ttcatcgtca atttttttgg atgaacgatc gtaatcatat cttctacctg tttccaggtt
    17941 ttgatttaaa gcaacctgtg accagtttgt agaaccgata agcacttcat tatcgtcaaa
    18001 gaccgcccat tttgagtgca atctttgcct gatgcgttta tctgttttat aaagtcttac
    18061 cggaatatta ttctttttta attcaatata accctttctg caaggaggga aactttctaa
    18121 tatatcagat gagactataa tttgagcgtc aagttcgcct tttttaactc tgtcaatgat
    18181 aatttcaatg agttcgtaat cagtaaaaac aaataattca gctcttagtt ttttacattt
    18241 cagcaattta ttttcaagat atcgtcttgt tgattcagtt ccttcatctc ctattaattc
    18301 aaactcttta ggctttgtac ccagaatttc catttgagga tcgtcaactg gatttgtgtg
    18361 aaccaaatcc agagtccctt ttttgtatct ggttttggct tcgggagtat tgtagaattc
    18421 ccctaaaagt ttttcataat caacattttc ttgtttgatg cgtttatcta taccgtggta
    18481 atatttttgt tctttttgag tcagaggacc tgcgacaaat tttgtttcac ccaacctttg
    18541 ccaagcaaac ttccaatctc tgttaaagtg ctcttcaata atgttgtcaa cttcagaatt
    18601 tttcttttta ggaagtgttt ctatggcaac gcaggcatcg tggttggcag gagaatggga
    18661 accccagttc ataccgccta atacagcttt tttaccatca acaacaagaa gtttaatatg
    18721 ctgaatcaaa gtcccctgct gagcccctct cggataagga acaacatcaa tgccattttt
    18781 ctttaagaac ttaatcatgt cttgatttgc ataatgtctt actctattgc cgttaccgtc
    18841 aatataccac ttgtgggcat caagaataac ctgaattttc atattcggat tggcttggcg
    18901 ttttttaata agtgtccata aaatttgctg ctgctctctt gcccctccaa ctcgctcagc
    18961 tccgttctga gcccacttat taccatcgac agttaaattt tggaactcaa acatttcaat
    19021 taaaatagac tcctgagcac tttttaaata atcaagagtt tttccaaaaa tttgctcccc
    19081 gtcaataagt gtttgaactc tggtattacc tacaatagcg gtcttacttt caaccggttc
    19141 aacttgtaag acatgcaaac ctttcaattt ttttcgcaaa aacgcatcgt tagtaagtcc
    19201 tgatttataa gcctctatat taatataatc agacatagac tgggttatat cagaaatagt
    19261 tgttaatttt cctgcatttt ggggcttaat cggattaata aagctttttt cagttgtaag
    19321 agttctttta tcattagcgc aaaaggctaa agaacttaaa ttataattaa aataactccc
    19381 gtaagagttt acgggagtat ctgttgaatt atttttttta cgggcataga aactgtgacc
    19441 tgtacaagaa acagccaagg ttgtaactcc tattttcata ttatccccgc aaaacccgtc
    19501 tataacattt ttattttaag gtttttttta tcaaaaagca atcaaatcaa gcttaaaaat
    19561 taacaaaact tacaacagag aataaaaaat atctataata tatctatttt ttataccaaa
    19621 ccttcacgaa cggcttttac tgcagcttga actctgtcat ttacacacat tttttgtaag
    19681 attgaacaca catgtgcctt tgccgtatga acacttacaa taagttcttt tgctatttcc
    19741 gtattgcttt ttcctgagac taagtgtttt aaaacttcaa attctctttc ggtaagaggg
    19801 actctggtac ctgaatcggc ttttgaaggg aaaatagaag tattttcagg cttagggaaa
    19861 acattaagaa cgagctgggc tacctgagga tcaatccaac aaacaccact tgaaacatct
    19921 cttacaacat cggcaagttt tataggatca atgtctttta agcaataccc atgagcacct
    19981 gatgcaagtg cagcaataac ctcttcctcc ctgtcatgag atgtcagggc aactatttta
    20041 gtatttggaa gcatttcctt aacccttata gtcgcttcaa ttccgttcat ctccggcaat
    20101 cccaaatcca taagaacaac atcaggctta tgtttttcaa ttaaatcaag tgcgctttta
    20161 gcattatcac attcagcaat aacacttata tcgggatttg aattcaaagc gcatcgcaga
    20221 cctaccctgg tgagtttaaa atcttcaaca ataataacct ttatctgctg accgcttttt
    20281 tttgtaaaat cagaataatc atctgtagta tttttattct taaataattg cttggtgttg
    20341 ctcataaaac tctcctgaaa tgtatcggca taaacggata gtgccttatt ttgagatagc
    20401 ctgataatgt aatttaacat tatagaaagc tacctgtata taagccaatc aggcaataaa
    20461 aatatgacaa tttaaattac ccaactggtt atatatcaag aaaaataaag tgtttttttt
    20521 aaaatatcag taaaaatgta tgtttcaaac agatacaata tattacaaaa atattaagtt
    20581 atataaatat atcacttgac aaaacaaaaa ctaattggta taatagtacc agttacaata
    20641 ttaatgaggc tggaatgaca ggcagtgtta taaaaatatc cgaagcggca tccttaggat
    20701 tacatgcaat gattattctt gctcaaaacc ctaaaaaact tgtttcggtt aaggagattt
    20761 ctgatttatt agaagtaagc gcaaatcact tatcaaaagt aatgcaaagg cttacaaaga
    20821 tcggcttcat aaattctgtc aaaggatata acggaggatt taaattaatt aaagctcccg
    20881 aaaaaataac ttttcttgaa atttatgaac tttttgacgg aaaattacca acttcaagct
    20941 gtcttttaac aactcacaaa tgcaaaggca attgtatttt aggagatttg gtcacctcgg
    21001 taaacaaaca agtaaaagaa aaattcgaaa aaacaaaact ttcggatttt gtaaaataat
    21061 ttttttaata ttaataagta aagtagtacc aatttattta aaaggagaat taaaaatgaa
    21121 acgagatatt ataaaaatag acgaaaaaaa atgtaacggt tgcggaaact gtattccggg
    21181 atgccccgaa ggggcgctgc agattataga cggcaaagca aggcttgtaa gcgatctttt
    21241 ttgcgacgga ctaggagcat gtataggaaa ctgccctgct ggtgccatga aaatagaaca
    21301 aagagaagct gaagcttatg acgaatataa agcaatgaaa aacattataa aaggcgggga
    21361 aaatgtaatt aaagcacatt tgaaacacct ggacgaacat ggagaactgg gctatttatc
    21421 tcaagcaatt gattttttag aacaaaaaaa cattcctgtt cctgactaca aaggaagaac
    21481 tcaatgcaac tgtccgagca caatgtcaaa aaaaatcgat agagaaaatt ttcctgaaaa
    21541 aacaataaat ttaactccac aattaaataa ctggcctata cagctagctt taatgaatcc
    21601 tgatgcacag tattttgaga atgcggattt attaatttca gctgattgcg taccgttcag
    21661 ctatccgaat tttcatcaaa aattcttaaa agataaggtt ttaataatgt tttgcccaaa
    21721 attagataaa acacttgaac aatatatcga aaagcttacc tacattttca aaaacaagaa
    21781 tattcaatcc gtttctattg tacacatgga ggtaccatgc tgctccggag ttgaaataat
    21841 aataaaaaaa gctttagaaa aagctcaaaa aataataacg attaaagatt atacaatatc
    21901 aatatcaggc gaaatagtat aaaaaggagg taaaaatgtc tttttatcaa tatgaacaaa
    21961 cagcaaaaag agaaaactgc cgactagcca gagcatgcca aaagcatttg caaagtgtat
    22021 atttacaaaa taaaattata gcaaaaatta aagaacttga attctacgca agtaaacaaa
    22081 aaaatataac taagctttct tgattttatc cggttttaaa caaaaaatgt tgcaatttta
    22141 ctgattttgc tgtatttttg aataagaaat attagacttg gacaatcatt gccggctttt
    22201 atcagcccta aaatactgaa tacactggta gataaatata atataaaccc atttttatat
    22261 acaaaatgat ttaaaagatg ttttgggtta gcaaaaaaca aattcagaag acggagtgta
    22321 aacaagtcaa aacaaggtaa atgcttgaat actcagtttt ttaaacttat ttttgttaag
    22381 aattattaaa ttttttcata aaattagcaa ttttaaaaat tcaggagtta ttaagtaagt
    22441 acaagtacag tattattatt ttttgaaagg aaaaaagaaa atgatgcaag ttccaacagt
    22501 acaaccccaa caagcttacg cacaacagcc aagctataac gcggtaaaaa ttgacgtaca
    22561 caatccacag gtaaatgcac ccggtgcaac acctgcacag gttccgtctt atgtaacagc
    22621 acctgtttac aatgtaccta atgcttcagt atatgaagtc ccgcaacaaa caatttacgg
    22681 accacaacaa aattttaatc aacccccggt cgcacaacag ccttcagttc cagcccctgt
    22741 agtagtaaca cctcaaccac aaacccctgt cgctcctgca gctgcacctc agcaacccga
    22801 agtaaaagct ccggaagccg cagctcctca agttgattta aatgcatttt taggaaaact
    22861 tggtggtgag aacttggatg aacaagcaaa tacaatggaa caaatcgcag aaataactca
    22921 aaataaccct caaaaagcct cagaacttct tgatacaaga atagttgatg cacttttagc
    22981 tataatgcaa aaagacacaa gcaaacttca aggcccaact caaaaacaac ttgaaatcag
    23041 agaaaaaatg atgaaaaatg aaactgtaac agatgcagaa aaagctgaag ccgaagccat
    23101 tacacctatg gaaaaagctg aaacaaataa acaatatgca atgtacacaa ctgcaatgct
    23161 tcaaaacctt tatatatcag aaattcaaaa aatgaacaac actgttgttc caatgacaga
    23221 acttcccgga gcagcaggca ttgttgaaca agttaaaaac aacccaaatc cgattgtaag
    23281 ggcatcaggt gttgattcat taagctacat tcaaagacct gaatacaaac aagatttgac
    23341 aacaatattt acaatagctc aaaaagacca agacaaatta gtccaagaag cagcaactaa
    23401 agcacttgaa aaaattaatc aagttgcagc agctcctgcc gcaacacctg caactgaagc
    23461 acctaaagct gaagccccaa aagctgaagc acctaaagca gcataatcta atttcctttc
    23521 atacaataaa aaagtgccgt ggattttatc cacggcactt tccttatttc atatgaaatc
    23581 tcaaaaggca ggcactgtgc ttgcagatta gttcaacaga agtcatcttc tgctcttttt
    23641 tatttttcag ggggcgcaaa tcaaaatttt cagattcttc tttttgtttt ttagacatgt
    23701 aatctttatt taaatcaata atagaacatg ccccaccggt tataccgtca taatcagaac
    23761 acgcccacac gggtactgtt aaaaccaagc atgccgtaaa aagaatcgtt aataaaatat
    23821 catataattt ttccatagct attatacact ttcagctcta ggattaaact tttaatcaca
    23881 cttactatgt ataataaagt attgaacaca acaaaacatt agaaaaaaga agaatttttc
    23941 aaaagcaata gcctgaaagt tgaatacaaa aaagtattat tcggcttttt tctttctgtt
    24001 tgtataaccg ttcacatagg catttacaac cgcttttgaa aaaacctttc ccgttgacca
    24061 gtaagaagta tgagccataa acacccctct tgttcccctg gtgtttgatt tatcgtaaac
    24121 aaatcctgca tggggttcaa tatcaagctt tgcgatttct tccatttctt ctattgataa
    24181 atttctgccg caaggaaatc ccaagggatc ttcacgataa ttaaccgtaa gccaaaacat
    24241 atcattttca attaaatatt caagtaaaag cctgttataa taagttattt gataattagg
    24301 gtctgaaata tccgaataaa aaagcaccaa agggcttgca aaattcacca ctcctttaag
    24361 tgagttatca ggagtacaga cctgatctgt caaattattc aaattcatat atttttcttt
    24421 aaaaagagag tcatttttat taggaactat atgaccggca gctgaaaaaa ccgccaaatc
    24481 ttcgcctaaa gccctcatgc aggtattttt catagggtgt tttgcgacaa attctctttg
    24541 agcgggtgta acatcaacat tgttaaagaa atcaaccgtt tcgatgaacg gaagtcttgt
    24601 aagcatatat tcataagtca caaaactgcc tgcggaataa ccatagagga caactttgtt
    24661 tcctttttca ttttctgaaa caaccatttt atgcaaatct tctacaacag gaagcatatt
    24721 gtgatacttc tgaacccaaa ttgcatcatg caaaaacctt gtaagtgcaa accttatctg
    24781 ataagcaatc cacggactaa aagtttttga aatatttaaa tcactttcaa caaaagccaa
    24841 atctctgtga cttttgtcac cccaaaagaa aattgcaggc tgttgttcaa taaaatattc
    24901 accgtttttc agaaaatatt cttgagttaa tttatttgac tcaaactctc ttttcatgtg
    24961 tggatgaagc ttttttactc cacgctcata ccaatttttc attttggcat cattattgtt
    25021 agagccgttt atatacaaaa aagttatccc tgtttgcgca aaacaagccg tttgaaacat
    25081 taaaaatgca gctaaaaaaa ttaaaatctt tttcatagca acattgtagc aaaaaaatta
    25141 tttatgaaaa ggataacctg cagcatcggc acatgcttcc ataaccgctt cactcaatgt
    25201 cggatgcgga tgaatcatat ttgccaggtc ttgcgccttt gctttcaact ttattgccag
    25261 agtcaattct gaaatcaact ctgacgcatt tgcacctaaa atatgcgcgc ctacgatttc
    25321 atctgaactc gcgtcaacta aaacttttac aaaaccttct ctatggccat tacaaagcga
    25381 gcggccgttg tgcctaaatt caaattttcc aatttttaat tttcttccgc cagcttcatg
    25441 ctccaccaat ccgatacttg caagttcggg gtgtgtataa atacaagaag gtacaacttc
    25501 attaaattta tttttatgcc cgcaggcatt ttctgctgcg attttgccct gagcaaaggc
    25561 aaggtgggca agaagcatgc cacctgtgac atctcctgcg gcaaaaaccc cttcaacatt
    25621 tgtttgcata aattcattta cttctattgc gccgtttttg gtttttatcc ctaaattcaa
    25681 gatatcatcg gataaattaa gcttcctgcc aactgcgaca agaacgtaat ttgaatttaa
    25741 atttatattt tgcccgtttt gttcaaaaat cgtgactaac tcgctatcaa cttcttttat
    25801 ctcatttacc cctgcaccca aataaaattt tataccgcgc cgtttcataa ttttaattaa
    25861 ttcttcgact gcttctttat cttcgtgagg aaggatttct tgttgaagtt caacgattga
    25921 aactttagaa cccagagcat taaaaaacgt tgcaaattca agccctatta cgcctgcgcc
    25981 tattattgta agattttgag gaatttcctg aatattaatc gcttcattgc ttgtgatgac
    26041 attttttaaa tcaattcctt ttatatttgg ctttagaggt tcagaaccgc ttgtgataat
    26101 taatttgtca aaactgacta aaaattcacc gctatctgct acaactttta taatattttt
    26161 agacaatata gtgccttttc cttttaaaat atttatattt ttactattaa ggagaaatt
//
LOCUS       DAOQAU010000019        25782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1885_length_25782_cov_6.847592, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000019 DAOQAU010000000
VERSION     DAOQAU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 25782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25782
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1885_length_25782_cov_6.847592"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PKI94_04320"
     CDS             <1..1310
                     /locus_tag="PKI94_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNW25999.1"
                     /translation="YPLECKKEFLKLVIENIYPNFCGIDLTIIADEVKDIQFEVEIPV
                     LKSPHPNPPPQGGREIPTPPIDIKEFFMPFSKNIFNFNINQLEGNFEQQSLSLREKAG
                     GVVETKLSENQDNCLPVALVSDGSAVLGFGNIGAAAAIPVMEGKAVIFKYLGGVDAIS
                     LCLKTQNPQEIITIVQALEKSFSGVNLEDICAPKCFDIEKALVERLNIPVFHDDQHGT
                     AIVVLAALLNALELADKKIEDVKIVISGAGAAAQAVSKLLLSAGAKNIVHTNRKGIVV
                     EGDENNDFYLEKLAQITNRGKIKGGLQDALKCADIFIGLSTAGVLKPEFIKEMKQKPI
                     IFALANPTPEIMPDEALKEGAFIVATGRSDFDNQINNSLAFPGLFKSLIVNKINKVTD
                     QIKLKCAIAIASLIKKEDLTAINILPNALDPRVPHAIINSFNN"
     gene            1450..1638
                     /locus_tag="PKI94_04325"
     CDS             1450..1638
                     /locus_tag="PKI94_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNW26000.1"
                     /translation="MARVLISMPEKFLDEIDSVAGNENRSRSELIREALRTYIHRNKV
                     RENVTATKNATILEALLD"
     gene            1924..2412
                     /gene="rimP"
                     /locus_tag="PKI94_04330"
     CDS             1924..2412
                     /gene="rimP"
                     /locus_tag="PKI94_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNW26001.1"
                     /translation="MPHENINRYEILEKITPLIENTAMRFGLIPIEIDFEKENHRWFL
                     RIFLFAKDRGVTLEDCENVTRSLNDFLDELIPCKYYLEVSSPGLDRKFKSDKEYLIFM
                     GKNISIKLKEPLEGEVDNIFRAKLLDYDEAKRKIFVKRLSDEKEMVLELDKIKNTRLY
                     IE"
     gene            2507..3766
                     /gene="nusA"
                     /locus_tag="PKI94_04335"
     CDS             2507..3766
                     /gene="nusA"
                     /locus_tag="PKI94_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNW26002.1"
                     /translation="MIKIGTALFEACEQLERERGISKEVLIDSLCDAMVAAYKKHMHI
                     KEATNIEAILDEQSGEIGVFRTKLVVKKVEEEMENEQISLKDAKEIDDEVELEDEVKI
                     EVTPDQFGRIAAQSAKQVITQRIREAERNLVLAEFMEKKGSLTTGIIQRVENRNVIVN
                     IGKTDAIIPQREQIPGEYYKSGNRIRVFVLNVKETTRLPQVIVSHAHAEIVRELFELE
                     VPEIEDGIVEIKSIAREAGYRTKIAVFSNDPEVDSVGACIGPRGSRIQTIVGELKNEK
                     IDIVRWSADPVEYIVNALSPARIVSVDILADDETSREALVVVPDDQLSLAIGREGQNV
                     RLAHKLTGWKIDIKSSTQMQNTENYPEYEEVVEDAGEDVEAEDEIKQEIEEELNEQVF
                     EADETAPELTVEAEEIEGTEEVEETEQ"
     gene            3945..5153
                     /gene="tuf"
                     /locus_tag="PKI94_04340"
     CDS             3945..5153
                     /gene="tuf"
                     /locus_tag="PKI94_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNW26003.1"
                     /translation="MAREKFERNKPHVNVGTVGHVDHGKTTLTAAITGAMAAAGKAQA
                     KKFDEIDAAPEEKARGITISTAHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGAMPQTREHILLARQVGVPKLVVFLNKCDAVDDPELLELVEMEVRELLTS
                     YELDGDNTPFIHGSALKALEAVAANPKIQRGEDKWVDKIWELMDAIDTYIPTPTRELD
                     KPLLMPVEDVFSITGRGTVATGRIERGRVKVGQEVEIVGFGETKKTTVTGVEMFRKLL
                     DEGLAGDNVGLLLRGVDKTEIQRGQVIAAIGSIKPHTKFTANVYVLTKEEGGRHTPFF
                     PGYRPQFYIRTTDVTGSIKFQGEMVMPGDNVVMEVELIAPVAIEAGMRFAIREGGRTV
                     GSGVVDKIIA"
     gene            5319..6026
                     /locus_tag="PKI94_04345"
     CDS             5319..6026
                     /locus_tag="PKI94_04345"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNW26004.1"
                     /translation="MGHFRLPLEGKTIMINSGHGAQKGPSLVDEGAKAKSGKKIIKEA
                     DLNDKVAINVKRKLTKLGAEVIYLDNMPLPMIRKIQEGVKADLFVSIHHDSKPAGFKH
                     NSGETIFAFGEKSTPLAQTINKWFKQDDTIPNNGINSAWAQQLYVLQSSNIIPSVLVE
                     VGHMSNKNELKILNKQEYQDIEAQNIVEGIRDFFRLPVKKKPQYKSSINNENFRKYKT
                     YKTTPPNPFFGHKDLIK"
     gene            6065..7036
                     /locus_tag="PKI94_04350"
     CDS             6065..7036
                     /locus_tag="PKI94_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26005.1"
                     /translation="MADFNLGNFLNKFVDASRAGLRGSSTEMTQKSKVAQETFNQNLN
                     AMQQKISVKPVILTPITRQPVVYQPLSMNHFDSLDRALYVKESMNLPKDLKEFLEIVQ
                     KANAGEQAPRLLAKNIDTNQIALFLQNQGKEAVNKLIGDMANASKQGISDLSQIKDTM
                     KLINACVSAAGEKSSSAIKNLILLYLPWIPLPQDKDFELEVETYENEKGESESSITIL
                     ISTKNYGNVKATLILGVGNSMTVLINCSEEFPKEELLKKLKAEGSAHAITSSIIFDQK
                     PAKQFENQSRQAKINMSNTTEINPFLLLMASYVIRYTIEIDNQKDIA"
     gene            complement(7039..7227)
                     /locus_tag="PKI94_04355"
     CDS             complement(7039..7227)
                     /locus_tag="PKI94_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNW26006.1"
                     /translation="MARILVSMPDDFLDKVDGLANDEQRTRSELVREALRNYIKRSKI
                     ANPKKAIENAKILDELLD"
     gene            7443..8135
                     /locus_tag="PKI94_04360"
     CDS             7443..8135
                     /locus_tag="PKI94_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26007.1"
                     /translation="MANSKLSEGVGKKIVEALKKQSEIEINPVSSVSAQKEESTFLDD
                     IPGFFEQASSAPQQKAEEPEDDFLASLESDSDLDLGSEQEFSSTTDFIQEEKMFAQEP
                     SVAPQTYSAPVSSQPLVSENFSKELDSFEIPSNIAVLKKLIGQLPSGVTRTTGAQIIK
                     QTMEALGISMRNVLQEAQQTQEGLKNSARECQNTIQEYKKQIIALEKQSQNYHRQYSA
                     LNELISLFVQTN"
     gene            8266..8940
                     /locus_tag="PKI94_04365"
     CDS             8266..8940
                     /locus_tag="PKI94_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26008.1"
                     /translation="MKKFILLLSFLLLSNSCALAQGVNGVIMNDIKEFLHIPAKKFAQ
                     KPPISDDEMTPSQQAILLYNDNDLKGAYEILLTVPDAKRNAQDYVLLGNILRDEDKIS
                     DAIFMYQRSILVDELFYKPYYNLGVLYLDEEKPFMAIENLKKACRLKKDFAYGYYNLG
                     CAYLKAGDLGKAKIAFLKAIELKNNEPDFYFNLAYTYKKLGKEKLSKQYLELYNKAME
                     LKGESI"
     gene            complement(8937..9827)
                     /locus_tag="PKI94_04370"
     CDS             complement(8937..9827)
                     /locus_tag="PKI94_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26009.1"
                     /translation="MGNMFSQELKEKYPFLIKYFEKGLKKPKRELAHSILFYGNDIEA
                     QYALATDLARILNCKELKNKDCKCINCNWIRDEKHPAVLTVSKFDNKPSDDDSKTVIS
                     VKQAQMIKNSLLNTSEFHRVFIFCDAKMEGEKRIPLGLNEQNFQEETANALLKTIEEP
                     PSNTTFFFLTRDKNDLIETIISRSQSFFVPSFEKPEKDFSAVSDIFENYPNIERKESL
                     DLAQSLFMLSKDIDSLNILKKIQNYMLALMKANIKNSALKTKILEDIKTVETAKKQIN
                     AKVNPQLAFENMCLNLTKPC"
     gene            10287..10490
                     /locus_tag="PKI94_04375"
     CDS             10287..10490
                     /locus_tag="PKI94_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26010.1"
                     /translation="MKEQELNTIMSVVSDPICNVYKVETRKDIEEIQRMVRIFNIAGG
                     QGNKHKMFSIKNMATMKLVLSNN"
     gene            10642..11169
                     /gene="rsmD"
                     /locus_tag="PKI94_04380"
     CDS             10642..11169
                     /gene="rsmD"
                     /locus_tag="PKI94_04380"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNW26011.1"
                     /translation="MIITGGKYKGRKVLLPDENIVRPTLSQIRESVFSSLFSITGKFE
                     GKSFLDAFAGSGIMGLEALSRGFEKIIAVEKNLKVVKILKENYLKLKIEPNILIGDTL
                     KKIEKFNETFDVIYLDPPYKSGIYELILKIIKEKKLLTANGIIVIEHDRELSFEGFEL
                     VKQKKYSGKFITFLK"
     gene            complement(11255..12574)
                     /gene="eno"
                     /locus_tag="PKI94_04385"
     CDS             complement(11255..12574)
                     /gene="eno"
                     /locus_tag="PKI94_04385"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNW26012.1"
                     /translation="MPTFIEDITARQILDSRGNPTVEVDVKLSCGVVGRAAVPSGAST
                     GIFEALELRDGDSSMYLGKSVLQAVDNVNTKIAPELIGEDASNQQEIDRIMLELDGTD
                     NKSKLGANAILGVSLAVAKASSLAYEMPLYRYLGGITACTLPVPMMNIINGGAHADNN
                     IDFQEFMIAPVGANSFQEAIRMGAEVFHNLKKVLHDRGLATSVGDEGGFAPNLGSNEE
                     GIEVILTAIEKAGYNTDQIKICLDVASSEFYKDGKYVLEGEGKTLSREEMVEFLAKWV
                     EKYPIISIEDGMAEEDWEGWKLLTEKIGDKCQLVGDDLFVTNTARLSEGIQRNIANSI
                     LIKVNQIGTLTETLAAISMAHEAGYTAISSHRSGETEDTFIADLAVATNCGQIKTGSA
                     SRSDRIAKYNQLIRIEQELGSAAKYLGLNAFNGQKGQNKSCCGCGCQ"
     gene            12685..13965
                     /locus_tag="PKI94_04390"
     CDS             12685..13965
                     /locus_tag="PKI94_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNW26013.1"
                     /translation="MKNAKIKSVILAAGKGTRMKSDLAKVLHKIFDKSLLGYVLDAVE
                     GTKLIDESFVIVGHQAKEVEKFVKSNYKKTSCVLQKQQLGTGHAVSMVSSALEKFDGE
                     VLILCGDTPLITADTLKNFIEFHKNKKSDLTVMSAVFDNPTNYGRIVRNGERLSSIVE
                     EKDASPAQKKIKEINAGIYCLNWQKVKKAFSELKSNNAQGEYYLTDIISWGGKCNLNV
                     NAFVLDDNEEIFGINSKLHLAQSYKMLNRKIVNKHMENGVTIVDPETTWISSDTKIGA
                     DTVIYPNVYITGKNKIGGSCSIGPFAHIRGDVELGDNVKVGNFVEVKKSKIASNTNAC
                     HLSYIGDSKIGSDVNVGAGTITANYNHFTKTKNKTKIKDGASIGSNAVLVAPVEIGKK
                     ATVAAGTVVTKDVEAQALAIARAPMRIIQNWPKK"
     gene            14054..14920
                     /locus_tag="PKI94_04395"
     CDS             14054..14920
                     /locus_tag="PKI94_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26014.1"
                     /translation="MIKPISKHLPAHIIDGIKEFYSKSHVRSSYSNPHAIHVNDSSGN
                     AEAKKEVSSFINKLGIKKNPKKFGPEASIGVKEDVYSKYPTRLFGFTNEIGAAVSPII
                     GPIGEMLTYIPALTYIFMDTADKYKRGEDFDYKKKSLRQGAERLAFQTFASVILPTGL
                     VKTVQALTDRTIDLKFLKNIKTGITRAVKQKPKLNNFFEMFSDLSLNNMPKGTLTKFA
                     LGFQKALNYITVFPLFFKNKGPKSGLRNFALVVVGITALASSIKIIDKFTEKVIIQKW
                     FNKIFHKKATAE"
     gene            complement(14926..16203)
                     /locus_tag="PKI94_04400"
     CDS             complement(14926..16203)
                     /locus_tag="PKI94_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW26015.1"
                     /translation="MLWYAINIFIIIIFIALFFITKKTNKKWSKINDYIGMVTNTVNS
                     VRYGNLSAKIARLDNPNCQNLSESINRMIETLKDRESMIIEYQNELTRQNKFLEAVIN
                     SLSDGILIIDEDYNILRATPQIANWFEEEGNNLLSKNIFDYLQVAENTKFEKLNNDEV
                     FIKNNNSASFEASIMKLNLDDKKKRFVLIIKDTTNQKELEQLREDFVATLTHDLKVPI
                     VAEANILDFLINGKFGEINEKQYEAISNMKTCNKELVDLVQILLETYKIKETGVDLNR
                     ENVYLNSFVWQVIEEMQPIAEKSEIKINYYVDGDFNVSADSMHLRRVIKNLIQNAISH
                     SNSNSDINVNIGYIPDFVTITITDYGQGISQENIDKIFNKYYSTAKKFRKIGTGLGLY
                     LSQQIAKSHGGEIVVTSQENVSTSFCIKIPSYK"
     gene            16269..16769
                     /locus_tag="PKI94_04405"
     CDS             16269..16769
                     /locus_tag="PKI94_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNW26016.1"
                     /translation="MKKFLLLATILLSSIQIVFAEVEAVSPELKEALKVSANEPGMFK
                     ILGTLLFVVGLIYVTGVIYSKLNIVGAKTVQEQLKKHDLSNVVILSTTQLGQGKNLHI
                     IELNKKRMLIGATQNSINLIRMLDDDETLPVKEEILEKKEKTLNDIQKQVSSSEEFDL
                     HKKYLS"
     gene            16782..17648
                     /locus_tag="PKI94_04410"
     CDS             16782..17648
                     /locus_tag="PKI94_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNW26017.1"
                     /translation="MRKTVKIADKIIGDGHPCFVIAEIGINHNGDVNLAKKLIDVAVM
                     SGCDAVKFQKRTVDVVYTKEELAKERQSPFGETNGDLKRGLEFGYEQYSEISKYCKKK
                     GIIWFASCWDEESVDFIEKFNPPCYKISSASLTDDALLKHTKSKKKPILLSTGMSTME
                     QIEHAVQVLGSDNLVIYHCTSTYPSDHEEINLKVIKTFREKFDCPIGFSGHERGLTPS
                     IVAVAMGANSVERHITMDRTLWGSDHAASLEPNGLFRMVRDIRQVPIMTGDGVKKVYE
                     SELPIIKKLRRK"
     gene            17648..18130
                     /locus_tag="PKI94_04415"
     CDS             17648..18130
                     /locus_tag="PKI94_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNW26018.1"
                     /translation="MSLKIPKTVKMVISDFDGVITDGGIYIDQNANISRKLNFKDIMA
                     ISLLKKAGIEVAFISGEKNSAIELISKKFSLKEVHQGIRIKIDVIKSIVERNGLKQSE
                     FLYIGDDINDIESLNYSSIKITVPNAVDAVKKVKDIQVTTNLGGSGAFREVADCLLSD
                     "
     gene            18112..19653
                     /locus_tag="PKI94_04420"
     CDS             18112..19653
                     /locus_tag="PKI94_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26019.1"
                     /translation="MSFVGLKNIKRNIKEIFNEILELDSGIDEYKKMVQNPYIPSVAY
                     VNWAVHLAEEGNFAEAEEKLVYSTLMAHQTPEAYINLGLLKAKDKDFNSAMMFFSKAI
                     RLDKNNSKAYCFLGNVLTELNDFKEAEKQFKIAQKLEPNNSDVVLNWGLSLVRQKKPL
                     LAREKFQQACKLNISNFNALFFWGLVELDLEELDKAREKFNLIISVMPNHSEAYYYLS
                     YIDFKEKKYDQSLKSALKSLDLNKFKMETYMIIAENYMNLKDEQKSFEYYEKGEVQAQ
                     VNYHFYNSWGLSLQGFGKFEESKEKFLKAIELNDKNEFAYAYLGISYYRTYNAEKAVE
                     YFEKTLNLNFENTVALQELGDINFERKNYQEAISYFEKVLKLSAKAIANYNKIANSYF
                     LEKNFAKANEYYKKAIEYQPLEIKPYIDYSKALVEQGDYKEALRKISAAYKLNDKNMD
                     CLNILFYVNYRLAKENLSDYNVEEAIKFAKIISDNYPDLFGYAKEKEELEEILNSKKS
                     ERQCR"
     gene            19672..20937
                     /locus_tag="PKI94_04425"
     CDS             19672..20937
                     /locus_tag="PKI94_04425"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNW26020.1"
                     /translation="MKRIVVQKFGGTSVADTNKIKEVAKAVIKEKNLGNDVVVVVSAM
                     GHTTDYLVKMAKEISKEPCAREMDMLLSTGEGVSIALLTMAIQAQGYDAISFNASQVG
                     IITEKVHQKARIVDIKTEKIKSKLSEGKIVVIAGFQGITTEGEITTLGRGGSDTSAVA
                     LGAALNASRCDLYTDVEGVYTADPRTVPCATRLKTVSYDEMLELSRVGANVLHPRSVE
                     TAKQFKVPLRVRSTFKLDNLGTLITGAKAMEMYQPVTGVAADLSQVRIVVCEVPDVPG
                     NAAKLFTRLAAENISVDMIIQSYARIDLNTNDIAFTIDKPDLEKTLIILEKVKEELKA
                     KAIHIDENIAKVSIVGAGMVDRPGIAATMFETLGKLDVNIKMISTSEIKISCLVEAQD
                     AQKSVKALHEVFELDCNEIAEVKGDLPEA"
     gene            21359..>21565
                     /gene="infA"
                     /locus_tag="PKI94_04430"
     CDS             21359..>21565
                     /gene="infA"
                     /locus_tag="PKI94_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNW26021.1"
                     /translation="MSKDVIELEGTILESMPNAMFKVKLENDHEILAHISGKIRKNFI
                     RILPGDRVKVEMTPYDLSKGRITFR"
     assembly_gap    21566..21665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21666..21779
                     /gene="rpmJ"
                     /locus_tag="PKI94_04435"
     CDS             21666..21779
                     /gene="rpmJ"
                     /locus_tag="PKI94_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNW26022.1"
                     /translation="MKVRSSVKKICDKCKCIKRKGRIAVICENPKHKQRQG"
     gene            21872..22243
                     /gene="rpsM"
                     /locus_tag="PKI94_04440"
     CDS             21872..22243
                     /gene="rpsM"
                     /locus_tag="PKI94_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNW26023.1"
                     /translation="MARLAGVDLPRNKRIIVALTYIYGIGPKRSQKILEATKISPDLR
                     TDNLTDEDIKQLRNELAKYNIEGDLRREVSMNIKRLQEINSYRGLRHKRKLPCNGQRT
                     KTNARTRRGKKGLAITKKKTV"
     gene            22332..22718
                     /gene="rpsK"
                     /locus_tag="PKI94_04445"
     CDS             22332..22718
                     /gene="rpsK"
                     /locus_tag="PKI94_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNW26024.1"
                     /translation="MARPTKTKKKEKKNVLQGIVHIQSTFNNTIVTSTDTQGNAIAWA
                     TAGATGFKGARKGTPFAAQSAAELVAKKSIDQGMKKVEVHIKGPGSGRETAIRAIQAA
                     GLEITLIKDVTPIPHNGCRPPKKRRV"
     gene            22796..23398
                     /gene="rpsD"
                     /locus_tag="PKI94_04450"
     CDS             22796..23398
                     /gene="rpsD"
                     /locus_tag="PKI94_04450"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNW26025.1"
                     /translation="MARYTGPTNKLFRNHGVKDLSNRKLVSSMKQAPSGQHGAARKKL
                     SDYATHLNEKQKIRLTYMLTEKQFAKCYHAAAGKKGVTGTILLQMLESRLDNVLYRAG
                     FGITRKQSRQIVNHGHVLVNGKKVDIPSYSVKAGDVIMVKSKSSDFIKAVTEMIDMAC
                     APAWMTIDKKALKITFDRTPEREELDPDFKEQLVIEFYSK"
     gene            23461..24432
                     /locus_tag="PKI94_04455"
     CDS             23461..24432
                     /locus_tag="PKI94_04455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNW26026.1"
                     /translation="MELKIKNVNTMTSSDGSQYGKFVIEPLEKGFGTTIGNSLRRVLL
                     SSLEGTAVTSVRIEGITHEYTAIPGVLEDVIDVMLNLKGLVVKTESKEPQHLRIDVDK
                     PGAVLASDIQLPAGVKVVNPDWLICTIADGGSFHADVMVETGKGYIANDVPRDSKAGM
                     SIDMLPIDATFMPIKRVSYNVEAARVGNSLDFDKLTLEIWSNGSIDVNNALSQSATFL
                     IEHFMQIASITGQPNITAAPVVAEETVEDESVPSITIEELELSVRAYNCLKRASINSM
                     AELLKKSENDLLNIKNFGKKSSDEVVERLRHFGLDLMPSPELVEEEV"
     gene            24464..24892
                     /gene="rplQ"
                     /locus_tag="PKI94_04460"
     CDS             24464..24892
                     /gene="rplQ"
                     /locus_tag="PKI94_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNW26027.1"
                     /translation="MRHQCKKHTLGKAQDQRKALLRSLATELFTHGEIKTTMARAKAL
                     RPYAESVISLAKKGDVHSRRQAGRYIFDKEIGKFIDPKTNEIFDVPPSDKKVVPQTVL
                     RKLFSQIGLKYSSRQGGYVRIYRLPPRRGDASEMALIQLV"
     gene            24944..25381
                     /gene="rplM"
                     /locus_tag="PKI94_04465"
     CDS             24944..25381
                     /gene="rplM"
                     /locus_tag="PKI94_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNW26028.1"
                     /translation="MKTYSAKPLEVERKWYLFDAEGEVLGRMATKIANILRGKNKPEY
                     TAHVDTGDFVVVINAEKIQVTGKKETDKKYYRHSGYPGGLKVASFKELMEKNPIAAIE
                     KAVKGMLPHNTLGDEQFQKLKVYAGSEHPHEAQKPIIIEKGGK"
     gene            25381..25782
                     /gene="rpsI"
                     /locus_tag="PKI94_04470"
     CDS             25381..25782
                     /gene="rpsI"
                     /locus_tag="PKI94_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002009728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNW26029.1"
                     /translation="MATQAKEIIATGRRKTSIAVVKLVKGKGRIYVNGKTLKNYIGNM
                     PAIELMIFKPLVLTDNQEKFDVRIKAVGGGICSQAGAIKHGIARALVKLNEEYKNVLR
                     SEGLLTRDPRVKERKKYGRKKARKRFQFSKR"
BASE COUNT         8982 a         4106 c         4965 g         7629 t
ORIGIN      
        1 cttatccgct tgagtgtaaa aaagagtttt taaaacttgt aattgaaaac atttacccca
       61 atttttgcgg aattgatttg acgattattg cagatgaggt taaggatatt cagtttgaag
      121 tggaaattcc tgttttaaaa tccccccacc ctaaccctcc ccctcaaggg gggagggaaa
      181 tacctacacc accaatcgat ataaaagaat ttttcatgcc gttttcaaaa aatattttta
      241 acttcaatat aaaccagctt gaaggaaatt ttgagcagca gtcgctttct ctaagagaaa
      301 aagcaggcgg cgtggtcgaa acaaaattaa gcgaaaatca agataattgc ctccccgtgg
      361 cgcttgtttc agacggttcg gcggttttag gcttcggcaa tatcggagca gcggcggcga
      421 ttcctgtcat ggaaggaaaa gctgtcatat tcaaatattt aggaggggtg gatgcgattt
      481 cgctttgcct gaaaacccaa aacccgcagg aaattataac tatagttcaa gcgttggaaa
      541 aatctttttc aggggttaat ttggaagata tttgcgcccc gaaatgtttt gacatagaaa
      601 aagcacttgt cgaaagactt aatattcctg tttttcacga cgatcagcat ggcacggcga
      661 tagttgttct ggcagcactc ttgaacgcgc ttgaattggc ggataaaaaa atagaagatg
      721 ttaaaatcgt aatatcaggt gcaggtgctg cagcacaggc agtttcaaag cttcttttat
      781 cagccggtgc taaaaatatt gttcatacaa atcgcaaggg gattgttgtt gagggagatg
      841 aaaacaacga tttttatctc gaaaaacttg cacaaattac aaatagggga aaaattaaag
      901 gcggcttaca agatgcgctc aaatgtgctg atatatttat aggactttcc accgcagggg
      961 ttttaaagcc tgaatttata aaggaaatga agcaaaagcc tataattttt gcgcttgcaa
     1021 atccgacccc tgaaatcatg cccgatgaag cattaaaaga aggtgctttt attgttgcga
     1081 caggcagaag cgactttgac aatcagataa acaattcact tgcttttccc ggtcttttta
     1141 aatcgcttat tgttaataaa attaataaag ttacagacca aataaagctc aaatgcgcaa
     1201 tagctattgc ttcactaatt aaaaaagaag acttaaccgc aataaacatt ttgcccaatg
     1261 cgcttgaccc cagagttcct catgcaatta taaactcttt taataattga ctttcagcca
     1321 attttattaa gaaatgtaaa tcaatattta caaagtatat ataaaatagg caatttttaa
     1381 atacaaaaat actttatata aatacgagga taaattaaga gagtacaagt tcaaaaggag
     1441 aaaaatatca tggcaagagt tttgatttca atgcccgaaa aatttttaga cgagattgat
     1501 tcagttgcgg gtaacgaaaa ccgttcacgt tcagagctta tcagagaagc cctgagaact
     1561 tatattcaca gaaacaaagt aagagaaaat gttacggcaa caaaaaatgc aactatttta
     1621 gaggcgctac tggattagta caaagatact aaaaacgata cgaaacgtta ttgggatttt
     1681 acgaggatta agagaaacga gattttaaaa gcggcatatg ccgctttttt atttggcttc
     1741 aaggcggcgc gccttgaagc accgtaacct gccgtaaaca ggctcaatgt aaatttttat
     1801 ttgtcatgta tttacaaaag aaatatttta atgtagtatc attttagtga ggcaattcta
     1861 aggaattgtt atcaggtcaa aaagaacggg catttgccgt tcttttttat ttaagggggc
     1921 aaagtgccgc acgaaaacat taacagatat gaaatattgg aaaaaattac accgcttatt
     1981 gaaaatacgg caatgaggtt tggcttgatt cccatagaaa ttgattttga aaaagaaaat
     2041 catcgttggt ttttgaggat ttttttgttt gcaaaagaca gaggtgttac ccttgaggat
     2101 tgtgaaaatg taacaagaag cctaaacgat tttttggatg aattaattcc ttgcaagtat
     2161 tatttggagg tttcatcacc cgggttagac agaaaattca aatccgataa agaatattta
     2221 atttttatgg gaaaaaatat cagcataaag ttaaaagaac cattggaagg cgaagttgat
     2281 aatattttta gggcaaagct tcttgattat gacgaagcta aaagaaaaat ttttgtaaaa
     2341 aggctttccg atgaaaaaga aatggttttg gagttagaca aaataaaaaa tacaagatta
     2401 tacattgagt gaatggtaaa tagtaaatag taaatggatt tttattctat tcactattta
     2461 ctatttccta tttacttaaa tacaaaaaaa gtaaggagta aaaacaatga ttaaaatagg
     2521 tacagcatta tttgaagctt gtgaacagtt agaacgtgaa agaggaattt caaaagaagt
     2581 tttgatagat tcactgtgcg acgcgatggt ggcggcttac aaaaagcaca tgcatataaa
     2641 agaggcaaca aatattgaag cgattttgga tgagcagtca ggtgaaatcg gggtgttcag
     2701 aacaaaactc gttgtgaaaa aagttgaaga agaaatggaa aatgaacaaa tttcccttaa
     2761 agatgcaaaa gaaattgatg atgaagttga acttgaagat gaagttaaaa ttgaagttac
     2821 acccgatcaa ttcggcagaa ttgccgcaca atcagcaaaa caggttatca cacaaagaat
     2881 aagagaagca gaaagaaacc ttgttttagc ggaatttatg gagaaaaaag gttctttgac
     2941 aacaggtata attcagcgtg ttgaaaatag aaatgttata gtaaacattg gaaaaacaga
     3001 cgctataatc cctcaaagag aacaaatacc cggagaatat tataaatccg gaaacagaat
     3061 tagagttttt gtgcttaacg taaaagaaac aacaagactt ccacaggtaa tcgtttctca
     3121 cgctcacgcg gaaatcgtta gagaactttt tgaattggaa gttcctgaaa ttgaagacgg
     3181 aattgtagaa attaaatcaa ttgcaagaga agccggatac agaactaaaa tagctgtttt
     3241 ctcaaatgac ccagaagttg attcggtagg agcctgcata ggacccagag gaagcagaat
     3301 tcagactatc gtaggcgaat tgaaaaacga aaaaatcgat atcgtaagat ggtcggcaga
     3361 tcctgtagaa tatatagtta atgcgctttc ccctgcaagg attgtttctg ttgatattct
     3421 cgcagacgac gaaacttcaa gagaagctct tgttgtggtt cctgatgacc aattgagcct
     3481 tgcaatcggc agagaaggtc aaaatgttcg tcttgcccac aagcttacag gttggaaaat
     3541 tgacataaaa agctccacac aaatgcaaaa tactgaaaac taccctgaat acgaagaagt
     3601 agttgaagac gcaggcgagg acgttgaagc cgaagacgaa attaaacagg aaatcgaaga
     3661 agaattaaat gaacaggttt ttgaagctga tgaaacagct cctgaattaa cggttgaagc
     3721 agaagaaatc gaaggaaccg aagaggtcga agaaacagaa caatagtgaa aaaggcttaa
     3781 atggtgaatg gttgaaaaat tttaataaaa atgctcttat tcacctatta acctattaat
     3841 ctatttaccc tctgttattg acattccaaa ttgagcatat aaaataagtg tatagcttat
     3901 aaaataagta aaaagtagta ataattaaaa aggagaccaa tctcatggca cgcgaaaagt
     3961 ttgaacgtaa caaaccgcac gttaacgtag gtacagtagg tcacgttgac cacggtaaaa
     4021 caacattaac agcagcaatt acaggtgcaa tggctgcagc aggaaaagca caagcaaaaa
     4081 aattcgatga aatcgatgct gctcctgagg aaaaagcaag aggtattacc atttcaactg
     4141 cacacgttga atatgaaaca gatgcaagac actatgcaca cgttgactgt ccgggacacg
     4201 cagactatgt aaaaaacatg attacaggtg ctgctcaaat ggacggagct attcttgtag
     4261 tagctgcaac agacggtgca atgcctcaaa ctcgtgaaca catcttgttg gcaagacagg
     4321 ttggcgttcc taaattagta gtatttttaa acaaatgtga tgctgttgac gatcctgagt
     4381 tgttggaatt ggttgaaatg gaagttagag aattgctaac ttcttatgaa ttagacggtg
     4441 acaatactcc atttatccat ggttcagcac taaaagctct tgaagcagta gctgctaatc
     4501 cgaaaattca aaggggtgaa gataaatggg ttgacaaaat ttgggaattg atggatgcaa
     4561 tcgatacata catccctact ccaacccgtg aattggataa acctttatta atgcctgttg
     4621 aagatgtttt ctcaattacc ggacgtggaa ctgttgcaac cggaagaatt gaaagaggac
     4681 gtgttaaagt cggacaggaa gttgaaatag tgggttttgg cgaaactaaa aaaactaccg
     4741 ttacaggtgt tgaaatgttt agaaaacttc ttgatgaagg tttagcaggt gataacgtcg
     4801 gtttattgct tcgtggtgtt gataaaactg aaatacaaag aggtcaggtt atcgccgcta
     4861 tcggttcaat caagcctcac acaaaattca ccgctaacgt ttacgttttg acaaaagaag
     4921 aaggcggacg tcacactcca ttcttcccag gatacagacc tcagttctac atcagaacaa
     4981 ctgacgttac gggttcaatc aaattccagg gagaaatggt tatgcccggt gataacgtag
     5041 ttatggaagt cgaacttatc gctcctgttg ctatcgaagc cggtatgaga ttcgctatca
     5101 gagaaggcgg aagaactgtt ggctcaggcg tagttgataa aattatcgct taagtagaat
     5161 ataatatcaa aaaggcgggc ttttttaaaa aagcccgcct ttttagtgtt taaattatgt
     5221 aaaattgtaa atttttgtat ttttctatac tctctttatt taagaataat caatttttaa
     5281 ataattaaaa cctttatata aatatagggg atatttgcat gggacatttc agattaccgc
     5341 ttgagggaaa aactataatg attaactccg gtcacggggc acaaaaagga ccttcgctag
     5401 tagatgaagg tgctaaggct aaatccggta aaaaaattat caaagaagca gatcttaatg
     5461 acaaagtagc tataaatgtc aaaagaaaac ttacaaagct tggtgccgag gtaatttatt
     5521 tggataatat gcctctgcct atgattagaa aaattcaaga aggagttaag gcggatttgt
     5581 ttgtttcaat tcatcatgat tcaaagcctg cagggtttaa gcataattcc ggcgaaacta
     5641 tttttgcttt cggagaaaaa agcactcctc ttgctcaaac tattaataag tggttcaagc
     5701 aggatgatac gataccaaat aacggcataa attctgcttg ggcacagcag ctttatgttt
     5761 tgcagtcttc aaatataatt ccctccgttt tagttgaagt cggtcatatg tcaaataaaa
     5821 acgaattaaa aattttgaat aaacaagaat atcaagatat tgaagcacag aatatcgttg
     5881 aagggataag agattttttc aggttgcctg tcaagaaaaa acctcaatat aaatcaagta
     5941 taaacaatga aaactttaga aaatataaaa catataaaac aactccgcca aatccgtttt
     6001 ttggacataa ggatttgatt aaataaaagg ttgctttttg tctaaaaatc agcataatgt
     6061 tagtatggca gattttaatt taggtaattt tttaaataag tttgtagatg cttcacgagc
     6121 agggttaaga gggtcatcaa ccgagatgac acaaaaatct aaagtggcgc aggaaacttt
     6181 taatcaaaac ttaaacgcca tgcagcaaaa aatttctgta aaacctgtta ttttaactcc
     6241 cataaccagg cagcctgttg tatatcagcc tttgtcaatg aatcatttcg acagtttaga
     6301 tagggcactt tatgtaaaag aatcaatgaa tttgcccaaa gatttgaaag aatttttgga
     6361 aattgtgcaa aaagccaatg cgggagagca ggcaccgagg cttttggcaa aaaatattga
     6421 taccaaccag atagctcttt ttcttcaaaa tcaaggtaaa gaggctgtga acaagcttat
     6481 aggtgatatg gcaaatgctt caaaacaggg tatttctgat ctttcacaaa taaaagatac
     6541 tatgaagctt ataaacgctt gtgtctccgc ggcgggggag aaaagttcaa gcgcaataaa
     6601 aaacttgata ttactttatc ttccgtggat tccgcttcct caggataagg attttgaact
     6661 tgaagtcgaa acatacgaaa atgaaaaggg agaatcggaa tcttctatta caattttaat
     6721 ttcaacgaaa aattacggaa acgtaaaagc aacgcttatt ttaggggtag gaaactccat
     6781 gactgttttg attaactgct ctgaagaatt tcccaaagaa gaacttttaa aaaaattaaa
     6841 ggcggaagga agcgcacatg caataacttc aagcattatt tttgaccaaa aaccggcaaa
     6901 gcaatttgaa aatcagtcgc gtcaggcaaa aataaacatg tcaaatacaa cggaaatcaa
     6961 tccgtttttg cttttgatgg caagttatgt tataagatat acaatagaaa ttgacaacca
     7021 aaaagatata gcttgatttt agtctaataa ttcatcaaga atttttgcgt tttctatagc
     7081 ttttttagga tttgcaattt ttgaccgttt gatgtaattt ctcaaagctt ctcttacaag
     7141 ttcgcttctt gttctttgtt catcatttgc caatccgtca actttatcta aaaaatcatc
     7201 aggcattgaa accaatattc ttgccattaa aacctcctgt aaaatacgtt taatttgctt
     7261 ttatttaata ctattgtaca tcaattccat atattttcta tatcttatcg gataaaaaat
     7321 aaaaatttga tatattttgt tacaaagtaa ttatttattt cacataaatt aatgttttct
     7381 gttaaaaaaa agttttttta ttataatgta taaatatgaa gaaatataag taggggaaaa
     7441 ttatggctaa cagcaaatta tcagaaggtg taggcaaaaa aatagtcgaa gcacttaaaa
     7501 agcagtcaga aatagaaatc aatcctgttt cgtcagtttc tgcacaaaaa gaggaatcga
     7561 cttttttgga tgatatcccc ggattttttg aacaagcttc ttctgcgcct caacaaaagg
     7621 cagaagaacc tgaagatgat tttttagctt cgcttgagtc tgacagcgac ttggatttgg
     7681 gaagcgaaca ggaattttct tcaactacag attttattca ggaagaaaag atgtttgcac
     7741 aagagccttc tgttgcgcct caaacatatt ctgctcctgt tagttctcag ccgcttgttt
     7801 ctgaaaattt ttcaaaagaa cttgacagtt ttgaaatccc gagcaatatt gctgttttga
     7861 aaaagcttat agggcagctg ccttcagggg ttacaagaac aaccggtgct cagattataa
     7921 agcaaactat ggaagctttg ggaatttcta tgagaaatgt gctccaagaa gcccaacaaa
     7981 cccaagaagg gcttaaaaat tccgcaagag aatgtcaaaa tacaattcaa gaatataaaa
     8041 aacaaattat tgcacttgaa aaacaatctc aaaattatca tagacagtat tctgctttaa
     8101 atgaacttat aagtttgttt gttcagacaa attaaagaat ccttgccaaa atcttgtttt
     8161 gacaaggact aatcagtaaa agagacatca cttatatctt ggcacatgtt ttttttgatt
     8221 tcaaatttct cgacatgaga aattatttaa actataattt tgtatatgaa aaagtttatt
     8281 ttattgctgt cttttttact tctttcaaac tcctgcgctt tggcgcaagg ggtaaacggg
     8341 gttattatga atgatatcaa ggaatttttg catattcccg caaaaaagtt tgcacagaaa
     8401 ccgccgattt cagatgatga gatgacaccg agtcaacagg cgatattact ctacaatgat
     8461 aatgacctga agggtgctta tgagattctt ttaacagttc ctgatgctaa aagaaatgct
     8521 caggattatg ttttgctggg gaatattttg cgagatgaag ataaaatttc agatgcgatt
     8581 tttatgtatc agcgttcaat cttagttgat gaactttttt ataaacctta ttataattta
     8641 ggggttttat atttggatga agaaaaaccc tttatggcaa ttgaaaattt aaaaaaggca
     8701 tgccgtttaa aaaaggactt tgcttacggt tattataatt tgggatgtgc gtatttgaag
     8761 gcgggggatt taggcaaagc aaaaatagca tttttaaaag ctattgaact taaaaacaat
     8821 gagcctgatt tttattttaa tcttgcctat acttataaaa aactcggtaa agaaaagctt
     8881 tcaaagcagt atttggagct ttataacaag gcaatggagc ttaaaggcga aagtatttag
     8941 cagggttttg tcaggttaag gcacatattt tcaaacgcaa gttggggatt tacttttgca
     9001 ttaatctgct tttttgcggt ttcaactgtc tttatgtctt ctaaaatctt tgtttttaaa
     9061 gcagaatttt taatatttgc cttcataagt gcaagcatat aattttgaat ttttttcaga
     9121 atatttaagc tgtcaatatc tttggagagc ataaaaagac tttgtgcaag gtctaaagat
     9181 tcttttcttt caatattggg ataattttca aaaatgtccg atacggctga aaaatctttt
     9241 tccggttttt caaaagatgg aacaaaaaag ctttgagagc gcgaaataat tgtttctata
     9301 aggtcgtttt tatcccttgt caaaaagaaa aaagtagtat tggacggagg ttcctcaatg
     9361 gtcttaagca aagcgtttgc ggtttcttct tgaaaatttt gctcattcag tccgagcgga
     9421 attctttttt caccctccat ttttgcatcg cagaagataa aaaccctgtg aaattcggaa
     9481 gtgtttaaaa gtgaattttt aatcatttgc gcctgtttta cagaaattac ggttttggag
     9541 tcgtcatctg aaggtttatt gtcaaatttt gatacggtta atactgcagg gtgcttttca
     9601 tctcttatcc agttgcagtt tatgcatttg caatctttat tttttaattc tttgcagttt
     9661 aaaattcttg caaggtctgt cgcaagagca tattgtgctt caatatcgtt tccgtaaaaa
     9721 agaatgctgt gggcgagttc tctttttggt ttttttaatc ctttttcaaa atattttatt
     9781 aaaaaagggt atttttcttt taattcttgt gaaaacatgt ttcccattct tgtttataaa
     9841 attattatat tataaatatt tgttatgttt atctttttgt aaacctactt tcttggaccg
     9901 ccaagaaagt aggtaaagaa actctccgac ctgatttgcg ttcattagct gacgctaaac
     9961 tcaacccgag ctcgcgaact cgtgcctttg gcactcaaac acactcgctt tgacattgtt
    10021 tcattaagcg tcagctattc actccaatat ggtcggaaag ggtttattaa aattgaattc
    10081 agggcgtagc cctgacactt tggcattttg ttcgcgtgtt tctctttctt tcgcccattt
    10141 ctttctcttt gcatcgcaat caaagagaaa gaaatgggca taaaaaaatc ttatattaat
    10201 tcttgtaaca taaaaagtaa taaaaaaggc aattatttgc ccatgattgt ttttattaat
    10261 atatagattg ttacgagagg cgacgcatga aagaacaaga attaaacaca ataatgtcag
    10321 tggtttcaga tcctatttgc aacgtttata aggttgaaac gagaaaagat attgaagaaa
    10381 ttcagcgcat ggtaagaatt tttaatattg caggcggaca gggaaataaa cataaaatgt
    10441 tttcaattaa aaacatggca actatgaagc ttgttttgag caataactga taagatttta
    10501 aaggggcggc gttttgcaaa gcaaaacgcc gccccttttt tttcgccccc tttacccctt
    10561 taccccttta cccctttacc cctttacccc tttacccctt tatcccttta tccctttatc
    10621 cctttttggt ataatctttt tatgattata acaggcggaa aatacaaggg gcgaaaagtt
    10681 cttttgcccg atgaaaatat tgtaagacct actttgtcgc aaataagaga aagcgtattc
    10741 agcagccttt tttcaataac gggtaaattt gagggcaaga gttttttgga tgcttttgcg
    10801 ggttcgggca taatgggatt agaggcactt tcaagaggat ttgaaaagat tattgcggtt
    10861 gaaaaaaatc taaaagttgt aaaaatttta aaagaaaatt atttaaaatt aaaaatcgag
    10921 cccaatattc taataggcga tactttaaaa aaaatcgaaa aattcaatga aacttttgac
    10981 gtgatttatc ttgacccgcc ttataaaagc gggatttatg aattgatttt gaaaataata
    11041 aaagaaaaga agcttttaac agctaatgga ataatagtaa ttgagcacga cagagaatta
    11101 agctttgaag gatttgaatt agtcaaacaa aaaaagtatt cgggtaagtt tataactttt
    11161 ttgaagtaaa cggaaaaagt ttaaaggtat aaaaatagtc gataagcttt acgtaactgt
    11221 tcaacttatc gactattcaa ctattcaact taatttattg gcatccgcat ccgcagcagg
    11281 atttattttg tcctttttga ccgttgaacg cgttaagtcc aaggtatttt gccgcagagc
    11341 ccagttcttg ttctattctt attaattgat tgtattttgc gattctgtca gaacgagaag
    11401 cagagcctgt tttaatttga ccgcagtttg tagcaacggc aaggtcagct ataaatgtat
    11461 cttctgtttc gccggagcgg tgagaagaaa tcgcagtgta gcctgcttcg tgtgccattg
    11521 aaattgccgc taaggtttct gttaaagttc cgatttggtt aactttaatt aagattgaat
    11581 tagcgatatt tctttggata ccttcagaaa gtcttgcggt atttgttaca aataaatcgt
    11641 ctccgactaa ttggcattta tctccgattt tttcggtcaa taatttccag ccttcccaat
    11701 cttcttctgc cattccgtct tcgattgaaa taataggata tttttcaacc cattttgcaa
    11761 ggaattcaac catttcttct ctggaaagag ttttcccttc tccttcaagg acgtattttc
    11821 cgtctttgta aaattctgat gaagcaacat ccaagcatat tttaatttga tccgtgttgt
    11881 atcctgcttt ttcgattgcg gttagtataa cttcgattcc ttcttcgtta gaacccaggt
    11941 ttggtgcaaa tccgccttcg tcaccgactg aagttgcaag ccctctgtca tgaagaactt
    12001 ttttaaggtt atgaaaaact tccgcaccca ttcttattgc ttcttggaag ctgtttgcgc
    12061 ccacaggagc tatcataaat tcctgaaaat caatgttatt gtcagcatgt gcgccgccgt
    12121 ttatgatgtt catcataggc acaggaagag tgcatgctgt aattccgcct aaatatctgt
    12181 ataacggcat ttcataagcc aacgatgaag cttttgcaac agcaagtgaa acgcccaaaa
    12241 ttgcgtttgc acctagtttt gatttgttgt ctgtgccgtc caattcaagc ataattctgt
    12301 caatttcttg ttggttggaa gcgtcttcac cgatgagttc cggtgctatt tttgtattta
    12361 cattatcaac tgcctgcaga acgctttttc ctaaatacat tgagctgtcg ccgtctctta
    12421 attcaagtgc ttcaaaaatt cctgttgaag ctcctgatgg tacagcggct cttcctacta
    12481 ctccgcaaga taatttgaca tcgacttcta cggtaggatt tccgcgtgag tccaaaatct
    12541 gtcttgctgt aatgtcttca ataaatgtcg gcatatttct ctccttctta aaagttacat
    12601 tttacgccca ttattatccc ataaacgctt tttatattca accttaaaat tttattgtat
    12661 aattatcgtg taaggagcta aagtatgaaa aatgctaaaa ttaaatccgt aattcttgca
    12721 gcaggcaaag gcaccagaat gaagtccgat ttggcaaaag ttttgcataa aatttttgat
    12781 aaatcgcttt tgggatatgt tcttgatgct gtcgaaggta caaagttaat agatgaatct
    12841 tttgttatag tgggtcatca ggcaaaagaa gttgaaaaat ttgtaaaaag taattacaaa
    12901 aaaacttcct gcgtgcttca aaaacagcag cttggaacag ggcatgcggt tagcatggtt
    12961 tcttctgcgt tggaaaaatt tgacggtgaa gttttgattt tatgcggcga tacgcctttg
    13021 attaccgcag ataccttgaa aaattttatt gaatttcata aaaataaaaa atccgactta
    13081 acggttatga gtgcggtttt tgataatccg acaaattacg gaagaatcgt tagaaacgga
    13141 gaaagattga gctccattgt tgaagaaaaa gacgcaagtc ctgctcaaaa gaaaattaaa
    13201 gaaattaatg caggaattta ttgtcttaac tggcagaagg ttaaaaaagc tttttctgaa
    13261 ttaaaaagca ataacgctca gggagaatat tacctcacag atattatttc ctgggggggc
    13321 aaatgtaatt taaacgtaaa cgcttttgtt ttggatgata acgaggaaat ttttgggata
    13381 aattcaaaat tacaccttgc tcaatcctac aaaatgctta acagaaaaat tgtaaataaa
    13441 catatggaaa acggggttac aatagttgac cctgaaacga catggatttc atctgataca
    13501 aaaatcggag cagatactgt gatttatcct aatgtttata taacaggaaa aaataaaatc
    13561 gggggtagtt gttcaatagg gccttttgct catataagag gcgatgtgga acttggtgat
    13621 aacgttaaag taggaaattt tgtcgaagtg aaaaagtcta aaatagcttc aaacacaaac
    13681 gcttgccacc tttcatacat cggcgacagc aaaatcggct ctgacgttaa tgtgggtgca
    13741 ggtactatta ctgcaaatta caatcatttt acaaaaacaa aaaataaaac aaaaatcaaa
    13801 gacggcgcaa gcatcgggtc aaacgcggtt ctggtagcac ctgttgaaat aggcaaaaaa
    13861 gcaacggtag cggcgggaac cgtagttaca aaagatgttg aggcacaggc tttggctatt
    13921 gcccgtgctc ctatgcgaat aatacaaaat tggcctaaaa aataaagaat tgtaaaaata
    13981 cttattataa aagcattttt tacgattcag gggttttata ttacactaga ttagtatttc
    14041 ttgggaaaaa ttaatgatta agccgataag taaacattta ccagctcata ttattgacgg
    14101 gataaaggaa ttttattcta aatcacatgt tcgttcatct tattcaaatc cacatgccat
    14161 acatgttaat gactcaagtg gtaatgccga ggcaaagaaa gaagtaagta gttttattaa
    14221 taaactcggt atcaagaaaa accccaaaaa atttggacca gaagcgtcaa tcggagtaaa
    14281 agaggatgtt tattcaaagt accctacaag actttttggc tttacaaacg aaataggtgc
    14341 ggcagtatct cctattatag ggcctatagg ggaaatgtta acttacattc ctgcactaac
    14401 atacatattt atggatactg cggataaata taaaagaggt gaagactttg attataaaaa
    14461 gaaaagcctg agacaaggtg ctgagaggct tgcttttcaa acatttgcaa gtgttatttt
    14521 gcccacaggg cttgtaaaaa cagttcaagc cttgacagac agaacaattg atttgaaatt
    14581 tttaaaaaat attaaaacag gaattactag ggcggtaaaa caaaaaccaa aattgaacaa
    14641 tttttttgaa atgttttcag atttgtcctt gaataatatg ccaaaaggca ctttgactaa
    14701 atttgctttg ggatttcaaa aagcacttaa ttatataact gtttttcctt tattttttaa
    14761 aaataaaggc ccaaaatcag gcttaagaaa ttttgctttg gttgtggttg gaataacggc
    14821 attggcttct tctataaaaa taatagataa atttacagaa aaagtcatta ttcaaaaatg
    14881 gtttaataaa attttccata aaaaagccac agctgagtaa gtttattatt tgtatgacgg
    14941 aatttttatg caaaagcttg tgctgacgtt ttcttgactt gtaacaacaa tttcgccgcc
    15001 atgtgatttt gcaatctgtt gcgaaaggta aagccccaat cctgttccta tttttctgaa
    15061 ttttttggcg gttgaatagt atttgttaaa gattttgtct atattttcct gagaaattcc
    15121 ctgaccataa tccgttattg ttattgttac aaaatcaggt atgtatccta tgtttacgtt
    15181 gatatcgctg tttgagttac tgtgtgaaat agcgttttga atcagatttt taataactcg
    15241 ccttaagtgc atactgtccg cgcttacgtt aaaatctccg tcaacataat aattaatttt
    15301 gatttcagat ttttctgcaa taggttgcat ttcttctata acctgccata caaaagaatt
    15361 caggtaaaca ttttctctgt ttaagtcaac gcccgtttct tttattttgt aggtttcaag
    15421 cagaatttgg accaaatcga caagctcctt gttgcatgtt ttcatattgg aaattgcctc
    15481 gtactgtttt tcgttaattt caccgaattt tccgtttatg agaaaatcca gaatgtttgc
    15541 ttctgcaaca atcggcactt tcaggtcgtg ggtaagagtt gcgacaaaat cctcccttaa
    15601 ctgttcaagt tctttttggt ttgtagtgtc tttaattatc aaaacaaagc gttttttctt
    15661 atcatcaagg ttaagcttca ttatgcttgc ctcaaaactc gcagaattat tgttttttat
    15721 aaaaacttca tcattattga gcttttcaaa ttttgtattt tcggcaactt gaagataatc
    15781 aaaaatattt ttgctcaaaa ggttgttgcc ttcttcttca aaccagttag caatttgcgg
    15841 ggtcgcgcga agaatattat aatcttcatc aataatcaaa attccgtcag aaagagaatt
    15901 tataactgct tctaaaaatt tgttttgtct tgtaagctca ttttgatatt caataatcat
    15961 gctttctctg tcttttagtg tttcaatcat gcgattaatg ctttcggaaa ggttttggca
    16021 attgggatta tcaagtcttg ctattttagc acttaaattc ccgtacctga cagaatttac
    16081 cgtatttgta accataccga tatagtcatt aatttttgac cattttttgt ttgttttttt
    16141 tgtaatgaaa aacagtgcaa tgaaaattat aattataaag atgtttatag cgtaccagag
    16201 catgtacaac tcccgtgtat tttgtgatac aataatttta tagcaaatat tttatttaag
    16261 gagttttaat gaagaagttt ttacttttag ctacaattct tttgtcctca attcaaatag
    16321 tctttgcgga agttgaggcg gtcagtcccg aattaaaaga ggcacttaag gtttcagcta
    16381 atgagccggg tatgtttaag attcttggaa cactgctttt tgtagtaggt ttgatttatg
    16441 tgacaggagt aatttattca aaattaaata ttgtcggtgc aaaaacagtc caagaacagc
    16501 ttaaaaaaca cgatttaagc aatgttgtaa tactttcaac gacccagctc ggtcaaggaa
    16561 aaaaccttca tataattgaa cttaataaaa aaagaatgtt aattggagca acacaaaatt
    16621 caataaattt aataagaatg ctcgatgatg atgaaacgct tcccgttaaa gaagaaattt
    16681 tagaaaaaaa agaaaaaaca cttaatgata ttcaaaaaca agtgtcttca tcggaagaat
    16741 ttgatttaca caaaaaatat ttgtcataag aggagcttaa tgtgagaaaa accgtaaaaa
    16801 tagccgataa aataataggg gatggacatc cgtgttttgt aatagcagaa atcgggatta
    16861 accacaatgg agatgttaat ttagcaaaaa aacttattga tgtggctgtt atgtccggat
    16921 gtgacgcggt aaaatttcaa aaaagaacag ttgatgtagt ttatacaaaa gaagaacttg
    16981 caaaagagcg tcaaagccct tttggggaaa caaacgggga cttaaaaaga ggtttagaat
    17041 tcggatatga acagtattca gaaatcagta aatattgcaa gaaaaaggga attatttggt
    17101 ttgcctcttg ttgggatgaa gaatcagtcg attttataga aaaatttaat ccgccctgtt
    17161 ataaaatttc gtctgcaagc ttaaccgatg atgccctgct taaacacact aaatcaaaga
    17221 aaaaaccgat actgctttcc acaggaatga gcactatgga gcaaatagaa catgcggttc
    17281 aagttttggg cagtgataat ttggttattt atcattgcac atcaacttat ccgagcgacc
    17341 atgaagaaat taatcttaag gtgataaaaa catttagaga aaaatttgat tgtcccatag
    17401 gtttcagcgg gcatgaaagg ggcttgactc cttctatagt ggcggtagct atgggtgcaa
    17461 attctgttga aaggcatata acaatggaca gaacactttg gggatctgac catgcggcaa
    17521 gcttagagcc aaacggactt ttcagaatgg taagagatat aagacaagtt cctattatga
    17581 caggagacgg agtaaaaaaa gtttacgaaa gtgaattgcc tataattaag aaattgagaa
    17641 gaaaataatg agtttgaaaa ttccaaaaac agttaagatg gttatttcag atttcgacgg
    17701 tgttattacc gacggtggaa tttatattga ccaaaatgca aacatttcca gaaaacttaa
    17761 ttttaaagat attatggcta tttcgctttt gaaaaaagca ggaatagaag ttgcgtttat
    17821 ttcgggcgaa aaaaattctg ctatagagct aatatctaaa aaattcagcc tcaaggaggt
    17881 tcatcagggc ataagaataa aaattgatgt gataaaatcg attgtcgaaa gaaacggctt
    17941 aaaacaaagc gaatttttgt acatcggaga tgatataaac gatatcgaaa gccttaacta
    18001 ttcatcaata aaaataacgg tgccaaacgc cgttgatgcg gttaaaaaag ttaaagatat
    18061 acaggttaca accaatttgg gcggaagtgg tgcttttaga gaggttgcag attgtctttt
    18121 gtcggattaa aaaatattaa aagaaatata aaagaaattt ttaacgagat tttggagttg
    18181 gactcaggga ttgatgaata taaaaaaatg gttcaaaatc cttacattcc ttctgttgcc
    18241 tatgtaaact gggcggtaca tttggccgaa gaaggtaatt tcgcagaggc agaagaaaaa
    18301 cttgtttatt ctactcttat ggctcaccaa acacccgaag cttacattaa tctggggcta
    18361 ttaaaagcaa aagataaaga ttttaattcg gcaatgatgt ttttttcaaa agctataaga
    18421 ctggataaaa acaattcaaa agcttactgt ttcttaggaa atgttcttac cgagcttaat
    18481 gattttaaag aggcggaaaa acagtttaag attgcacaaa aattagagcc gaacaattct
    18541 gatgtcgtct tgaattgggg gctttctttg gtaaggcaaa aaaaaccgct tttggcaaga
    18601 gaaaaatttc agcaggcttg taagcttaat atatcaaact ttaacgccct tttcttttgg
    18661 ggtttggttg agcttgattt ggaagaatta gacaaggcaa gggaaaaatt taatcttata
    18721 atttctgtta tgccaaacca tagcgaggca tattattatc tttcatatat tgattttaaa
    18781 gaaaaaaaat atgaccaaag tcttaagtcg gcactaaaat cccttgattt gaataagttt
    18841 aaaatggaaa cttacatgat tattgcggaa aattacatga atcttaaaga tgaacaaaaa
    18901 tcttttgaat actacgaaaa aggagaagtt caagctcagg ttaattatca tttttataat
    18961 tcttgggggc tttcacttca aggtttcggc aaattcgaag aatctaaaga aaaattctta
    19021 aaagcaatag aacttaatga caaaaatgaa tttgcttatg cttatttggg aatttcttat
    19081 tatagaactt ataacgcaga aaaagcggtt gaatattttg aaaagacatt gaatcttaat
    19141 ttcgaaaaca cagttgcgtt acaggaatta ggggatataa attttgagag aaaaaattat
    19201 caagaagcta taagttattt tgaaaaggtt ttgaaattgt ctgcaaaagc tattgccaat
    19261 tataacaaaa tagcaaattc gtattttctc gagaaaaatt ttgcaaaagc taatgaatat
    19321 tataaaaaag caatagaata ccagccgtta gaaatcaagc cttatataga ctattcaaaa
    19381 gcgttggttg agcagggcga ttacaaagag gctttgcgta aaatttctgc tgcatataag
    19441 cttaatgaca aaaatatgga ttgtctgaat attttatttt atgtaaatta caggcttgcc
    19501 aaagaaaacc tttctgacta taatgtagaa gaagcaatta aatttgcaaa aataatatca
    19561 gacaattacc ccgatttgtt tggctatgcc aaagaaaaag aggaattgga agaaatatta
    19621 aatagcaaaa aatcagaaag gcaatgcagg taatcttgta aaagagcaat tatgaaaagg
    19681 atagttgtac aaaaattcgg cggtacttca gttgcggaca caaataaaat aaaagaagtg
    19741 gcaaaagccg taataaaaga aaaaaatttg gggaacgatg ttgtagtagt tgtttctgca
    19801 atggggcata caacagatta ccttgttaaa atggcaaaag aaatttcaaa agaaccctgc
    19861 gcaagagaaa tggatatgct cctttcaaca ggcgaaggtg tttcgattgc gcttttgaca
    19921 atggctattc aggcacaagg ttatgacgcg ataagtttta atgcctcaca ggttggtata
    19981 attacagaaa aagtacacca aaaagcaaga attgttgata taaaaacaga aaaaataaaa
    20041 agtaaattaa gcgaaggcaa aattgttgta atagcaggtt ttcaaggaat tacaacagaa
    20101 ggtgaaataa ctactctggg aagaggcggc tctgacactt ctgcggttgc tttgggtgcg
    20161 gcacttaatg cttcaaggtg cgatctttat accgatgtag aaggggttta tactgcagat
    20221 cccagaacgg ttccttgtgc aacaaggtta aaaaccgttt cttatgacga aatgcttgaa
    20281 ctttcaagag tcggtgcaaa tgttttacat cctcgttctg ttgagactgc aaaacagttt
    20341 aaggtacctc tcagggttcg cagcactttt aaattagata atttaggaac tttaattaca
    20401 ggagcaaaag ctatggaaat gtatcagccc gtaacaggcg tggcggcaga tttatctcag
    20461 gtcaggatag tagtttgtga ggttcccgat gttccgggta atgctgcaaa actttttaca
    20521 agacttgctg ctgaaaatat ttcggttgac atgattattc agtcttatgc aagaattgat
    20581 ttgaatacaa atgatatagc ttttacaata gacaagccgg atcttgaaaa aacgcttata
    20641 atccttgaaa aagttaaaga agaacttaaa gcaaaagcaa ttcatataga cgaaaacatt
    20701 gcaaaagtct caatcgtcgg tgcgggaatg gtggacagac ccggtattgc agccactatg
    20761 tttgaaactt tagggaagct tgatgttaac attaagatga tttctaccag cgaaattaaa
    20821 ataagctgcc ttgtggaggc acaagatgcc caaaaatccg taaaagctct gcatgaagtt
    20881 tttgagcttg attgtaatga aatcgccgaa gtcaaaggcg atctgcctga ggcgtaattg
    20941 tttgaagggc ttaaaaggtg aataggtgaa aagtttataa aagtgactga ttgagcaggg
    21001 aactaagtga ctaagaaaat ttataacttg ctttctttcc ttctcgtcct cttatcacct
    21061 ttcatttctc acgatttact attctcggtt gttttattaa acattaaata attaattatg
    21121 taaacaaatt gttacatagc ggcttgaaag tgtttatttg atttaaaaat tccagcttgc
    21181 tcatagtctg tttgtgttgc atttataaaa aaacaaccgc ctcctgcggt tctatttact
    21241 atttactatt tactatttac tgccgtttcc cccttgcttt cagattttta ccatgtatga
    21301 ttgattatgc actagtattt ttgaaatatg agcaagttta aaaaacaaag aggaatttat
    21361 gtcaaaagac gtaatagaat tagaaggtac gattttagaa tcaatgccta acgctatgtt
    21421 caaggttaaa cttgaaaatg atcatgaaat attggcgcat atttccggaa aaatcaggaa
    21481 aaattttata agaattttgc ccggagacag agttaaagtg gaaatgacac cttacgattt
    21541 atcaaaaggc agaataacct tcagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnatgaa agtaagatca tcagttaaaa aaatttgcga taaatgcaaa tgtattaaaa
    21721 gaaaaggccg cattgcggta atttgcgaaa accctaaaca taaacaaaga caaggatagt
    21781 gaacggtgaa cggtgaactg agattttctg ttcacacttc acagctcact aaatcataaa
    21841 gttaaaaaat acaaacaagg agcaaacaaa tatggcaaga ctggcagggg tagatttacc
    21901 tcgtaacaaa agaataatag tagcattgac ttatatctac ggtataggtc caaaaagaag
    21961 tcaaaaaatt cttgaagcta caaagatttc accggatttg agaacggata acttaactga
    22021 cgaagacatc aaacagttaa gaaacgagtt ggcaaagtat aatattgaag gtgacttaag
    22081 acgtgaagtc agcatgaata taaaacgtct tcaagaaatc aattcataca gagggcttcg
    22141 ccataagcgc aaacttcctt gtaacgggca aagaacgaaa acaaacgcac gcacaagaag
    22201 aggtaaaaag ggcttggcaa taaccaagaa gaaaacagtt taaaagtgaa gcgtgaaggg
    22261 tgaagagaaa aatccaatca ctcttcatct aatcataaga ttaacaaata taaacaagga
    22321 gcatataaaa aatggctaga ccgacaaaaa caaagaaaaa ggaaaagaaa aacgtattac
    22381 aggggattgt ccacatacaa tcgaccttca acaatacgat agtaacttcc acagatactc
    22441 aaggaaatgc tattgcttgg gcaacagcag gtgctacagg atttaaaggc gcaagaaaag
    22501 gaactccttt tgctgcgcaa tcggcagcag agcttgttgc aaaaaaatca atagatcagg
    22561 gtatgaaaaa agttgaagtt catattaaag gaccgggctc aggcagagaa actgctatca
    22621 gagcaattca ggctgcagga cttgaaatta cgttgattaa agacgtaact ccgattcctc
    22681 ataacggatg ccgtccgcct aaaaaacgta gagtgtaacg agtgaacggt gaacagtgaa
    22741 cggaaaaatt tcagctcaca gctcaccaaa ataaaaacaa ggagcaaata aacaaatggc
    22801 tagatataca ggaccaacaa ataaattatt ccgtaaccat ggggttaagg atttatcaaa
    22861 cagaaaactg gtttcgtcaa tgaagcaagc cccttcaggg cagcacggtg cggccagaaa
    22921 aaaactttct gattacgcta cgcacttaaa tgagaagcaa aaaatcagac ttacttatat
    22981 gttgacagaa aaacaatttg caaaatgcta ccatgcagct gccggaaaaa aaggcgttac
    23041 cggtactatt ttattgcaaa tgcttgaatc aagacttgat aacgttttat acagggcagg
    23101 ttttggcatt acacgcaaac agtcaagaca aatcgtaaac cacggacacg tgcttgttaa
    23161 cggcaaaaaa gttgacattc cttcttattc ggttaaggca ggcgatgtga ttatggttaa
    23221 atcaaaaagt tccgatttta ttaaagcggt tacagaaatg attgatatgg cttgtgcacc
    23281 tgcgtggatg acaatcgata aaaaagcact taaaataact tttgacagaa ctccggaaag
    23341 agaagaactc gatcctgatt tcaaagaaca gttagtaata gaattttatt ctaaataggg
    23401 gcaaatatat tattatttta agaaaataca aataagaaat aacgacagga gaaaataaat
    23461 atggaattaa aaattaaaaa cgtcaatact atgacaagtt cagacggttc acagtacggc
    23521 aaatttgtta tcgagccttt ggaaaaaggt tttggtacaa caatcggaaa ttccctgaga
    23581 agagttttgc tttcaagtct tgaaggtact gcggtaactt ctgtaagaat tgaaggaatt
    23641 actcacgaat acacagctat tccgggtgtt ttggaagatg taatcgatgt tatgcttaac
    23701 ttgaaaggtt tggttgtaaa aaccgaatca aaagaacctc agcatttgag aatagatgtt
    23761 gacaaaccgg gcgcggtttt ggcaagtgat attcaacttc ctgccggagt taaagttgtt
    23821 aaccccgatt ggttaatttg tacaatcgca gacggcggaa gcttccatgc agacgttatg
    23881 gttgaaaccg gaaaaggtta tatagcaaat gatgttccaa gagattcaaa agcgggaatg
    23941 tcaattgata tgcttcctat tgacgcaaca tttatgccta ttaaaagagt aagttacaat
    24001 gtagaagctg ctcgtgtagg aaattcactt gattttgaca aattgaccct tgaaatctgg
    24061 tcaaacggct cgattgatgt aaataatgct ttaagccaat ctgctacatt tttgattgaa
    24121 cactttatgc agattgcttc aattacagga cagcctaata ttacagctgc accagttgta
    24181 gccgaagaaa cagttgaaga cgaaagcgtt ccaagcatta caattgaaga attggaatta
    24241 tcggttagag cttataactg cttgaaaaga gcaagcataa actctatggc tgaactgctt
    24301 aaaaaatcag aaaatgattt attgaatatt aaaaactttg gtaaaaaatc ttctgatgaa
    24361 gtagtagaaa gacttcgcca cttcggattg gatcttatgc caagccccga attggttgaa
    24421 gaagaagtat agtaaataaa aaaactaata aaggacacaa atcatgagac accaatgtaa
    24481 aaaacatacg ctgggaaaag cccaagatca aagaaaagca cttttgcgtt ctcttgcaac
    24541 cgagcttttt acccacggtg aaataaaaac aacaatggca agagcaaaag cactcagacc
    24601 ttatgcagaa agtgttatat cccttgctaa aaaaggtgat gttcactctc gtcgccaagc
    24661 gggcagatat attttcgata aagaaatcgg taaatttatt gaccctaaaa caaacgaaat
    24721 tttcgatgtg ccgccaagcg acaaaaaagt agtaccgcaa accgttttga gaaaactctt
    24781 ttcacaaata ggattaaaat attcttcaag acagggcggt tacgttagaa tatacagact
    24841 gccgccaaga cgcggtgacg cttcggaaat ggctttaatc caattagtat aaggctaggc
    24901 gaggcaaaat taaaataaaa aataaacaag gagcatacaa aaaatgaaaa cttactcagc
    24961 aaagcctctt gaggtagaaa gaaaatggta tttatttgat gccgaaggcg aagttctcgg
    25021 cagaatggct accaaaatag caaatatcct aagaggaaaa aacaagcccg aatatactgc
    25081 gcatgttgat acaggcgact ttgtggttgt tatcaacgca gaaaaaattc aagttacagg
    25141 taaaaaagaa acagacaaaa aatattacag acactcaggt tatcctggtg gacttaaagt
    25201 tgcttcattc aaagaattaa tggaaaaaaa ccccattgct gcaatagaaa aagcggttaa
    25261 agggatgctt cctcacaata ctttgggcga tgaacaattt caaaagctta aggtttatgc
    25321 aggaagtgag catccgcatg aagcccaaaa acctattata attgagaaag gaggtaaata
    25381 atggcaactc aagcaaaaga aattatcgca acaggccgca gaaaaacctc tatcgcagtt
    25441 gttaaactgg ttaaaggcaa aggcagaatt tatgtgaacg gaaaaactct taaaaattat
    25501 atcggaaaca tgcccgctat tgaactaatg attttcaaac cgttagtttt aacggataat
    25561 caggaaaaat tcgatgtaag aattaaagct gtaggtggcg gaatctgttc tcaagcaggt
    25621 gctatcaaac acggtatagc aagagctttg gttaaactta atgaagaata taaaaatgtg
    25681 cttcgctcag aaggtttatt gacaagagac cccagagtca aagaacgtaa aaaatatggt
    25741 agaaagaaag cacgtaaaag attccagttc tcaaaaagat aa
//
LOCUS       DAOQAU010000020        25564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1918_length_25564_cov_8.088753, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000020 DAOQAU010000000
VERSION     DAOQAU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 25564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25564
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_25564_cov_8.088753"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKI94_04475"
     CDS             <1..394
                     /locus_tag="PKI94_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26030.1"
                     /translation="RGTIEDTIIGDDTKIDDQVMIGHNCRIGKGCMIISQVGIAGSCV
                     IGDRVVIAGQVGIADHINIGNDCIIAAKSGVSKSFPEKTIVIGYPAMPRKEFIRQLKT
                     MKDADELIKKFKKYEPLLKTFEEGNAEA"
     gene            413..1222
                     /locus_tag="PKI94_04480"
     CDS             413..1222
                     /locus_tag="PKI94_04480"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNW26031.1"
                     /translation="MTTILKKIEVKGNGLMKNRECCVQILPSSKGKIRFFVEESKEPI
                     IFDVESVISTDHCVVLGNGKTKVMLTEHFSAACAFLGIDSLDVHISKPEMPILDGSAK
                     EWVELFKKAGYEKSEKVSYSVKEPVYYLNGKTHLVIIPDKELNITYSVQYDHPELDHT
                     WVSFNGRGEDEIISARTFGYLKDLRKFQLFGFARGVTMENTVGLNGESYTTKLRSCFE
                     PVKHKILDLIGDLYLTGVNPLDLKAQIIVKEAGHAVHIKAAKMLKNKLIKI"
     gene            1293..1739
                     /gene="fabZ"
                     /locus_tag="PKI94_04485"
     CDS             1293..1739
                     /gene="fabZ"
                     /locus_tag="PKI94_04485"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNW26032.1"
                     /translation="MSDKILSFDTMEIMAMIPHRYPFLLVDRITECVPGEYSKGYKNV
                     TMNDEFFQGHFPGNPIMPGVLQIEAMAQCSAPILLCLDNFRGMLPLFASMENVRFKNI
                     VRPGDKFEMEIQLLKIKGPIAKCHAVGSVDGVVTVEADMTVCMKQA"
     gene            1842..2648
                     /gene="lpxA"
                     /locus_tag="PKI94_04490"
     CDS             1842..2648
                     /gene="lpxA"
                     /locus_tag="PKI94_04490"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNW26033.1"
                     /translation="MIDKSAVMHKSAQIEKDVFIGPNVIIGENVKLASGVKILANAYL
                     EHCEIGEGTIINPFASIGTAPQDLGYKNEPTKVIVGANCLIKENVTINRAANCGDMTT
                     TVGNKCLLMVGSHVAHNCKLEDEVILANLVTLGGHVHVGFGAFIGGMSVFHQNLRIGE
                     MAIISGFSAARQDILPYSKGEGRPPYPAGINAIALKRRGVAQEERTAINEALKLLISP
                     EYNTSQAIEQIEKTLKINPYVQKMIDFVRTSKRGVTLQCRKSGHRILEDE"
     gene            complement(2652..3692)
                     /gene="queA"
                     /locus_tag="PKI94_04495"
     CDS             complement(2652..3692)
                     /gene="queA"
                     /locus_tag="PKI94_04495"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNW26034.1"
                     /translation="MLTSDFDYNLPEELIAQLPCEKRCESRMMVLEKNSKKIAHKHFF
                     DIVDFLDENDVLVLNNTKVIPARLFGQKLTGANVEVFLLKDLGNNHWEALLKPSKRIN
                     GGNIIKISPELGVKAIRLIEGGKWLIEFLCKGHVRQILQKVGNIPLPPYIERNLSNED
                     IKKLDFERYQTVFAQKEGSVAAPTAGLHFTKEILEKLEKKGVQIAYVTLDVGLGTFRP
                     VKCENILEHKMETETFEITEETAKIINEAKKNKKNIVAVGTTSVRTLETAFQKYGEIK
                     ACKGFSELFIYPPYEFKVVDKLLTNFHLPKSTLLMLTSAFAEREFVLEAYKNAITEKY
                     RFYSYGDCMFIY"
     gene            complement(3772..4854)
                     /locus_tag="PKI94_04500"
     CDS             complement(3772..4854)
                     /locus_tag="PKI94_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNW26035.1"
                     /translation="MDFVTLTIEALRALSNIVGNYGIGIILLTIVVRLFMWPLSVSQQ
                     RSMKTMQTLQPKLKAIQDRYKSDPQTMQRKMMEFYKEHQFNPMSGCFPLLLQMPIFIL
                     LYSALMSPQFIQIAGDTQFLFIKRLDATLKTSAGVSFDGTMNVSKYDTFYLGKTAKVY
                     LPKETIENVKIEKPNKALEVQGEINPGQPIDFKVSLDSLDLKFSQLDQIQKAELTITN
                     AQNRESEKVEFARKNGLLIYSMPTKEVKSSLHYDVLLLIVLFGLTMVATQKIMMASAG
                     SQNQDPTQQAIQKSMGAFMPIMIMATFVFIPIPAGVLLYLISSNVIQVIQTVVINKQL
                     EIEDEKKKQKIDDDAVKTAKKIEPKE"
     gene            5183..5259
                     /locus_tag="PKI94_04505"
     tRNA            5183..5259
                     /locus_tag="PKI94_04505"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5217..5219,aa:Pro,seq:tgg)
     gene            5930..6181
                     /locus_tag="PKI94_04510"
     CDS             5930..6181
                     /locus_tag="PKI94_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26036.1"
                     /translation="MINIPKINILRAVTQTDVKIELKSRRNMKKYFPNFHKLCIEEGL
                     YTKAGFKIKEYFRQRTKQFSKMFSEQEHRDRIDGCFSKK"
     gene            complement(6185..7609)
                     /locus_tag="PKI94_04515"
     CDS             complement(6185..7609)
                     /locus_tag="PKI94_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW26037.1"
                     /translation="MNIHARSRCETKIKAPIVEALEAAVKNPTYQFHIPGHTKGKGTY
                     PEFKKLIGQKALNIDTTDEFDNLGTLHPATGPIKEAQELAAQAFGAKKTFFLLNGSTA
                     GNLAIAMGLTKKGQKIITNRNCHRSLLTGMIISGAEPLWLIPQRLDEWGIWGSVSPES
                     VENLLKENQDVSLVWITNPTYEGVVSDIKSISLICKKYNVPLIVDEAHGSLWKFNKHL
                     PSSSLETGADVVVQSLHKTGGSMSQSSMLHIGKNSIIDDSKIEKALKLLHTTSPSLIL
                     LASLDAARANLQSPLGKKQIARAVNNAKYLRTRIDSLPNIHQLKSDFGYKTDVTKVFI
                     KADGLSGKRLESILEIDFKIEVESASDIGILILSNIGNKRSDFEYLADCLEKISNTNY
                     TDICYMENKKHMPLTTPKIKMSMQEAFYSQKETVSRENSVGRICAEVIAECPPGISIL
                     LPGELISAQHLAYLEDYDFIEVVK"
     gene            7729..8943
                     /locus_tag="PKI94_04520"
     CDS             7729..8943
                     /locus_tag="PKI94_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26038.1"
                     /translation="MKKNNILKLLILFCSLICINSVYADETIDSAKTEKTSKIDLKAV
                     IFDPEFDNKEEIKVEELSDADKIKYNLHEALHGEVKAISTKGLFADKMTVKFDEGPIK
                     SIVPWIDYNGSFSNVWTGDNYTNTFYGINFADVGFNINMRDDKTFVRVMFSPVRYVEG
                     NTYFQSFFADNYITRKISKNNTLLVGHTWVPLGIEGKGSPLILDFFTRSQTSRTYSSV
                     RALGTKLMGNYKYADYQVGFYSSGRFFKDFFPGPEVAAWVDFKPLANTKGEYGKLTVS
                     GGFDGGNAQSNYAVGTAAVNYEYKRLRLTTEMGIADGSNGATGFTSNKSGGVNTTLTY
                     RVTPKTQVLFRFDQFDPNTEKANDVRKEYTAGINYFIKDEALRLIMNFTHYEIENGAF
                     GDRIMVGTQIIL"
     gene            9082..9750
                     /locus_tag="PKI94_04525"
     CDS             9082..9750
                     /locus_tag="PKI94_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW26039.1"
                     /translation="MNTGQVKVLIVEDHKLMRVGLKSLFEDFPEISIVAEAESGKEAI
                     EKIRLTKPDVVLMDIGLPDMTGIEATKKILEQFEGVRVIMLTSHTTEQEVLDSLSAGA
                     NAYVLKDINTEILMMIIKTVKEGAMWLDPQIVPIIREKNCGVIPQKQMSRANFKAQHS
                     NLTEREYEVLKLVVDGKSNHDIAKELVISEHTAKAHVCNIIQKLVVDDRTQAAVKALK
                     EGLV"
     gene            complement(9747..11132)
                     /locus_tag="PKI94_04530"
     CDS             complement(9747..11132)
                     /locus_tag="PKI94_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNW26040.1"
                     /translation="MEQLVTNKTLEKLKYDLVREGLVDYDTVEKAQELANAQNINVGQ
                     ALINSGILEEEKLLKFLEAKLHIPYVNLEDYALDKNCLKFINFSDAQKYKIIPLFKIE
                     DVLTIAMADPLDLFAIDKVVETTGCSIEPVISSEISILKKIDEYYKTENAVGEIFTDS
                     ENINYDWREELHSEDLSDEHIQKIIRAILKQAIFQNVHEVYFEYTKEGLGVNFKQNSE
                     VFDKGYIPSVLTSSFVSKLKTLSNLDPAVSEVPQLGKLCFKVDDMTLIASISAFPTIL
                     GERISLKIYKPPKNLRKIITDEGGLNIIKNSLNEPGIVLVCGSALSGKTHMIYSLLLE
                     IAGSGKNIMTIESIAKYNLENVNQCEMNENIGFNLDKALRFIEFQSPDIIYLEGVKTK
                     EAFDYFSSLVFKEKIVITEFLANNMDELRKKTAFGEFEMFKSLINCMIFIHSTDSIEV
                     FDKSTIQKYLA"
     gene            complement(11183..11551)
                     /locus_tag="PKI94_04535"
     CDS             complement(11183..11551)
                     /locus_tag="PKI94_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW26041.1"
                     /translation="MKKILIVDDEQDIVESLKFVLEAAGFKCYCAYNGEEGLQMAKDV
                     LPDLIILDVMMPKINGYKICRLLKYDSKYKNIPILMVTARSQDEDKLIGEETGVDEYI
                     TKPFELEEIVKKVEKYLGKG"
     gene            complement(11651..13723)
                     /locus_tag="PKI94_04540"
     CDS             complement(11651..13723)
                     /locus_tag="PKI94_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNW26042.1"
                     /translation="MAKILFLTDNHKKCKLFSDMIDKNPHDFLCAFTINEAVEKTKLQ
                     NPDLVILDAEVKSFDLKSAHKKIKAFNDNLQTILVTKTDDIDPELLKISSAFLKEPVA
                     KDFLLATINANIKTKNSLEMLSDSNRELARSLYQLNVLYNTSSQFAGTLDKNKLIDFM
                     VEGLDKSLSFGLACVLTFRSENEPVLIINSLYQISDRLLEAIKLRTILNYKSLFEKKE
                     MPFELNIENLKIEKHFKHTMKEYDFSVLRYDNIFAPINLSEKFFGFIEIYRENDFTTE
                     DTTCFQTIVQQVSLPLKSASLYEEIKKTNQKLERLERLKSEFISIVSHELRTPLTAIK
                     NSLDILLSGKSGILTETMDKFINMAKRNVTRLSGIINDLLDLSKIEAGKMDFKFALTT
                     IEPVIDYVKASLEEMAKEKGLVIVSRIEDNKTKIYADAHRLEQVLTNLVSNAIKFTNE
                     GGKIEVCSQVVDAQEIQIQEGFEEELKKLKGKYIVVRVKDNGIGIAYKDLAHVFDKFA
                     QIENSLSRKVGGSGLGLPIARQLLDAHNGAVWCNSKPEKGSSFYFAIPVANEINNFML
                     EFKQAIQKTKVSNSVLTLVKMQGEKDLITNLLEDKHLINKNYLKSSIQETESKNKTVT
                     MVIPDGDKFSADFLKKKIQTYLKDHENLFPKCAIMYSYATYPNDGADEWELMKKVDET
                     LQKLRGVT"
     gene            13935..14498
                     /gene="rpsP"
                     /locus_tag="PKI94_04545"
     CDS             13935..14498
                     /gene="rpsP"
                     /locus_tag="PKI94_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNW26043.1"
                     /translation="MLKIRLKRLGAKKTPAFRIIVINSTTKREGRPIQELGHYNPKTK
                     VMKLDKTSALEWVKKGAQPTETVQYLIDNCNTDGSLNYKKKTEKKLSKKAIAKLEAEK
                     EAKAKAKEEEKAAKEAPKAQEPAQEPVVEEAKVEAAEEPKAVEETPVEAVVEEVAVEE
                     APVKEEAAVETVVESAAEEVVEETTEA"
     gene            14655..15062
                     /locus_tag="PKI94_04550"
     CDS             14655..15062
                     /locus_tag="PKI94_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26044.1"
                     /translation="MNVPKMGHVSFGNAYLIQTRNPKVPEAFLMIDTNLRAQDNPALR
                     WEHILTSKTDQYVKGGLLLTGDEMLEYHRLEKKFKSGRGEIKEKDFYHKLTKMVNKFK
                     EIAQKIRFDSDVDSLTKQIPEVSTIAKDLKKFI"
     gene            complement(15072..15515)
                     /gene="dut"
                     /locus_tag="PKI94_04555"
     CDS             complement(15072..15515)
                     /gene="dut"
                     /locus_tag="PKI94_04555"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNW26045.1"
                     /translation="MYTTTLKIEKLAHNTYLPEYQTEGAAGMDLCAAIENPVLLKPFE
                     RKLIPTGIKIELEHGFEAQIRPRSGISIKHGISLVNCVGTIDEDYRGEVCVPVINLST
                     EEYTILPNERIAQMIIARVEKAKIEVVPSLSETSRAAGGFGSTGV"
     gene            complement(15540..16070)
                     /locus_tag="PKI94_04560"
     CDS             complement(15540..16070)
                     /locus_tag="PKI94_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW26046.1"
                     /translation="MRYKRLFIPNTYIHLVIVTYKRQPMLIKNIDLLRNSFKNAKQFF
                     NFDIFAICILPDHIHMILKPENIHEYPKIITSIKYYFSNNIDVGVETPTYGYKNKGER
                     GIWQRRYYEHTIRDEEDLNKHIDYIHYNPVKHNHTKNVKDWEFSSFEKFVKMKNYEQN
                     WGSSDDIKHIETLDYD"
     gene            complement(16131..18899)
                     /gene="alaS"
                     /locus_tag="PKI94_04565"
     CDS             complement(16131..18899)
                     /gene="alaS"
                     /locus_tag="PKI94_04565"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNW26047.1"
                     /translation="MISGNEIREQFLKFFKEKHNHTVVESSSLVPDNPTVLLTTAGML
                     QFVPYFLGLEKPKFPRATSCQKCARAGGKDSDIENVGRTPRHHTFFEMLGNFSFGDYF
                     KKEIIQWSWDFVTNYLKLDKEKLWVTIYKDDDEAFEIWTKVVGLSPERVIRKGKKDNF
                     WGPPGPTGPCGPCSEIHYDLGEEFKCSPDCGIATCECDRWVEIWNLVFMEMFQDEDGK
                     LSPLEKKNVDTGMGLERITMVCQGKTSTFETDLLNPILDEICKMSNVKYKQSDKTDVS
                     LRIITDHARCVTFLINDGIIPGNEGRNYVLRMILRRALRHGKMLGLELPFLYKLVDVV
                     VKNYGGAYPDLIKNAEKIKNTIKNEEEQFNKTLDRGYKLLEELLSKKQGGKCAISGED
                     AFKLYDTFGFPLELTVEIAKENGFKVDLKEFEKEMEEQKKRAKAAAQKVVLTDDLKYV
                     EIEKQFGSTEFLGYEKEEIEAKIIAKVEGDNFVDIILDKTPFYAESGGQVGDTGVLEN
                     SNCKVSVLTTFKVNHLFVNRCEIIEGEVKIGDMVNAKIDSARREEIKIHHTNAHLLQA
                     ALKKVLGNEVKQAGSQVEENRTRFDFSYPRQVSYNELKRIEKIINDWIGQNLEVSTKV
                     MKLEDAQKSGATALFGEKYENDVRVVSIGTISKELCGGTHIDNIGKLRLMKIVSESAI
                     AAGTRRIEAVAGKTALEYLNDKEDVIDKIAGHLKTPYEEVFERIEKIQEENKSLQKEL
                     ASVQNAMAKEKFASYVDKAEDIEGGKLFISKIDAMDSNALKEGVEYLAQRLGDSIIVI
                     MSDSTIVAKVSDKFVANGINAGQIVSNISQLLGGKGGGRPQFAQGGIGNVGIVGVETP
                     TYKNSLTDREEEILEQIVFGKSNTEIAKIFDITITEAKTHVCSILQKLSVQDRVQAAV
                     KAIRDGLV"
     gene            19283..19356
                     /locus_tag="PKI94_04570"
     tRNA            19283..19356
                     /locus_tag="PKI94_04570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19317..19319,aa:Arg,seq:tct)
     gene            19542..19615
                     /locus_tag="PKI94_04575"
     tRNA            19542..19615
                     /locus_tag="PKI94_04575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19576..19578,aa:Arg,seq:cct)
     gene            20050..21063
                     /locus_tag="PKI94_04580"
     CDS             20050..21063
                     /locus_tag="PKI94_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HNW26048.1"
                     /translation="MIISVNLFNYNQNGFKINKSPVFFGANLVSPKEVQNFIRLISKQ
                     KLQENMLVLTSPETQGRAIGTQGIEIASQFIEAQFEKIGLEPFCQIGLKTFSQPFLAY
                     QPKLLFEENLKNIYGWIRHKSNPPEIPAKNIVGIIRGKNPEKYLMLTAHYDHLGSIPE
                     KNLIFPGANDNASGVSVLLELARIFKKIKQPEKSIIFAALSDEEGYKKGAQTLAQSLI
                     DNEIHSIDIINMDMLGGQKGNTIDLWQENKKLSVNIVGAFKQTCDTLGLKLRLHRGDP
                     CSDATMFNIEGMPVVCASWDFLSKVNTRFFHTNADTVDKINPEILEKAAKAIGTASLN
                     LIC"
     gene            complement(21127..21417)
                     /locus_tag="PKI94_04585"
     CDS             complement(21127..21417)
                     /locus_tag="PKI94_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26049.1"
                     /translation="MEIKEQLIVLRKIFYKCFVVGLFFLILAAVIYYPCKCYVASIYQ
                     SAFGVDVITYQNLWVIFVGLIKTILIFLFLVPALAIHWVTNCYEKEEKDSSE"
     gene            complement(21492..21971)
                     /locus_tag="PKI94_04590"
     CDS             complement(21492..21971)
                     /locus_tag="PKI94_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26050.1"
                     /translation="MIESKRELYPQYRFTSDRRQMNMPVDVERRSGMDRRFQDRIVID
                     QKLDRDIFEIKSKAVQIQHTEPIKFRQNAAKALQNNIKTDEFIKTEKPENKASKDDFS
                     DKTPSASAAGAFAFILGGVLISTMFGVSGIAIALGLGAYFGLRLFAEAMHSHMKNKD"
     gene            22074..22340
                     /locus_tag="PKI94_04595"
     CDS             22074..22340
                     /locus_tag="PKI94_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26051.1"
                     /translation="MKKIILFLCLLISAPVLSQEAEGFAADSQGASLQDKPDYNKSVD
                     KEVQGSDDFFDELIYPQYGSSQSNDFEGRQDQNPTESNYGYDGS"
     gene            22356..22982
                     /locus_tag="PKI94_04600"
     CDS             22356..22982
                     /locus_tag="PKI94_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNW26052.1"
                     /translation="MHVIAINGSPKSEGNTYNALKLVLEELKNQGISGEIIHIGNKNI
                     RGCLACGGCFKNKDESCVIKDEVNECIQKMKEADGILIGSPVYYASIAGTLKCFLDRT
                     FYVCSANGGLLRHKVGASVVVARRSGEVAAFDHLNHYFTIAEMFMPSSNYWNVIHGRM
                     PGEVLLDEEGVQIMRVLGKNMAYLLKQLKTGTQTAPEQEQKMLTNFIR"
     gene            22997..24310
                     /locus_tag="PKI94_04605"
     CDS             22997..24310
                     /locus_tag="PKI94_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26053.1"
                     /translation="MKRFKKIFILLLFFLLNICNYAFSQNNYSNAAIQIYNSGVNFHK
                     QHNFDLAEQKYFQVLKIQPNFIEAKNNLALLYYNKAVNNLSSSNYTDSIVYAQKALSY
                     GYKPLECYNIIANCYMQMKDYENLILIGNKLDTLSPNDEFILNYLALAYMNTNQNEKA
                     RDIYQKILLINPDDKAAQQNIKYINYCHSQKVLNNSINNLPTVAHAPASLYRLIKPSA
                     GITQGTVDKMKYILDLIWSEPNGQILLQEFLNKKIPIKISQGTVTANAMLQNKKNTLL
                     LYGFIPVFSYDTSSISVNIAFNYISDFFNPNIDSGHRIYDLQVFVHEFGHAFMLCKNS
                     GHRDSIEEELGVSMIGFNSAYKIIIGKGLTKEQTEEYSLNVLQSLLSDEHRNLPVYNG
                     FVSEIQRYGIVMPYPEIYSNLPAMYKKLLCEEKISPVPNFYVYNR"
     gene            24415..25449
                     /locus_tag="PKI94_04610"
     CDS             24415..25449
                     /locus_tag="PKI94_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW26054.1"
                     /translation="MKIVFFDIREDETEAFGALCKGERKGKCQIECTCKMLKETLNEQ
                     TVLTDEIKQAEVISCFTFSRVTENVLKQFPNLKLIALRCVGFNHIDVEYCRKNNIQVL
                     NSFGYGNITVAEFAFALLFDVSRKVTRAYINLKEGELSKDFYTGFELNGKTIGIIGTG
                     AIGTQAIRIAKGFGMNVLGYDPFPKKELVEKYQVKYVELDELLKNSDVVSLHSPLTED
                     NFHLINEEKINLMKSTAVLINTARGELIDSKALYGALSENRILGAGLDVLEAENMITQ
                     PDTILDFDYLTDDYIRQTLINERLLKLHNVVVTPHIAYNSKEAKERIVSITMDNIDKF
                     FQGEIQNSVL"
BASE COUNT         8077 a         4564 c         4569 g         8354 t
ORIGIN      
        1 cagaggaacc attgaagata cgattatcgg tgatgacaca aaaattgatg atcaagtcat
       61 gataggtcac aactgcagaa taggtaaagg ctgtatgatt atttctcaag taggcattgc
      121 aggaagctgt gttattggag atagagtagt aatcgcagga caggtaggaa ttgcggatca
      181 cataaatata ggtaatgatt gtattatagc cgcaaaatca ggagtttcaa aaagctttcc
      241 ggaaaaaaca atagtaatag gctatccggc aatgccaaga aaagaattta tacgccagct
      301 taaaacgatg aaagatgcgg atgaactcat taaaaaattc aaaaaatacg agccgctttt
      361 aaaaacattt gaagaaggaa acgcagaggc ttaagccccc cgaaggttaa caatgacgac
      421 tattttgaaa aaaattgagg taaaaggcaa cggcttgatg aaaaatcgtg aatgttgcgt
      481 tcagatttta ccttcttcaa aaggtaaaat aagatttttt gtggaagaat cgaaagagcc
      541 cataatattt gacgttgaaa gcgtaatttc aactgaccat tgcgttgttt taggtaacgg
      601 caaaacaaaa gttatgctta cagagcattt ttctgctgcc tgcgcatttt taggaataga
      661 ttcgcttgat gttcatattt caaagccgga gatgccgatt ctcgacggaa gtgctaaaga
      721 atgggttgaa ttgtttaaaa aagcaggtta cgaaaaaagc gaaaaagtca gctacagtgt
      781 aaaagagcct gtttattacc ttaacggcaa aacgcatctt gtgattattc ctgataaaga
      841 acttaacata acgtattccg tccaatatga ccaccccgaa ctcgaccata cgtgggtttc
      901 tttcaacggc aggggcgaag atgaaatcat aagcgcaagg actttcggat atttaaagga
      961 tttaagaaag tttcagcttt tcggctttgc aagaggcgtt acaatggaaa acacagtagg
     1021 tttaaacggt gaaagctata caaccaaatt aagaagctgt tttgaacctg ttaagcataa
     1081 aattcttgac ttaatcggag atttatattt gactggagtt aatcctttag acttaaaggc
     1141 tcagattata gtcaaagaag caggacatgc agttcacatc aaagccgcaa aaatgcttaa
     1201 aaataagctg ataaaaattt agatattaaa aataaattaa aaaaacaaaa aaagaaaata
     1261 attgtgataa tgtaaaaata aggagatata taatgtcaga caaaatttta agtttcgata
     1321 caatggaaat catggcgatg attcctcaca ggtacccgtt tttacttgtt gacagaatta
     1381 cagaatgtgt ccccggcgaa tattccaaag gatacaaaaa cgttacaatg aacgacgagt
     1441 tttttcaggg gcattttccg ggaaacccta ttatgcccgg agttcttcaa attgaagcca
     1501 tggcgcaatg ttctgctcct attttattgt gcttggataa ttttaggggt atgctgcctt
     1561 tgtttgcatc aatggaaaat gttaggttta aaaacattgt aagacccggt gataaatttg
     1621 aaatggaaat tcaattgttg aaaataaaag gtcctatcgc gaaatgtcat gccgtgggtt
     1681 ctgttgacgg agtagtaaca gtggaagctg atatgacggt ttgcatgaaa caggcttaaa
     1741 agctgaaagg gtgaaaagct gaaaagttaa atttattata aatgtacttc tttacttatt
     1801 cacctattca cttaatcacc taaaaacaga aagaaggaaa aatgattgac aaaagcgcgg
     1861 ttatgcacaa gtccgcacaa attgaaaaag atgtatttat cggccctaac gtaataatcg
     1921 gggaaaacgt taagcttgca agcggagtta aaattcttgc aaacgcttat ttggaacact
     1981 gcgaaatagg cgaaggcact ataatcaatc cctttgcaag cataggaaca gcccctcagg
     2041 atttaggcta taaaaacgaa cccacaaaag ttattgtcgg tgcaaactgc ttgataaaag
     2101 aaaatgttac aataaaccgc gcggcaaact gtggggatat gaccacaaca gtcgggaata
     2161 aatgcttact tatggtaggt tctcacgttg cccacaactg caagcttgaa gatgaagtaa
     2221 ttcttgccaa tttagtcacc cttggcggac atgttcatgt cggattcggt gcgtttatcg
     2281 gcggcatgag tgtttttcac caaaatttaa gaataggtga aatggcaatt ataagcggat
     2341 tttcagctgc tcgtcaggat attttgcctt attcaaaagg tgaaggaagg ccgccttatc
     2401 ctgcgggaat aaacgctatt gcgcttaaac gacgcggagt tgcacaagaa gaaagaaccg
     2461 ccataaatga agctttaaag ttgttgattt ctcccgaata caacacatct caggcaatag
     2521 aacaaattga aaaaacgtta aaaataaatc cttatgtcca aaaaatgatt gattttgtaa
     2581 gaacttccaa aagaggcgtt acccttcaat gccgcaagtc cggccacagg attttagaag
     2641 acgaataatt tttaataaat aaacatacaa tcgccgtagc tgtaaaatct gtatttttca
     2701 gttatggcgt ttttgtaggc ttctagaaca aattcacgct ccgcaaaagc acttgtaagc
     2761 atcaaaagcg ttgattttgg caggtgaaag tttgttaaaa gtttatcaac tactttaaac
     2821 tcataagggg ggtaaatgaa caattctgaa aagcctttgc atgcttttat ctcgccgtat
     2881 ttttggaaag ctgtttccaa agtcctgaca gaagtcgtgc ccaccgctac aatatttttt
     2941 ttatttttct ttgcctcatt tataattttt gcggtttctt ctgttatttc aaaggtttca
     3001 gtctccatct tgtgttctaa aatgttttcg cacttcacgg gtctaaaagt tccaagcccc
     3061 acgtcaaggg tgacataggc aatttgaact cctttttttt caagtttttc aagaatttct
     3121 tttgtaaaat gtaaacccgc agtaggtgct gcaacagaac cttctttttg ggcaaaaacc
     3181 gtttgataac gctcaaagtc aagttttttt atatcttcgt ttgacaaatt tctttctata
     3241 taaggcggaa gtggaatgtt tccgactttt tgcaaaatct gtcttacgtg tcccttgcaa
     3301 agaaattcta taagccactt tccgccttca ataagcctga ttgctttaac tccaagttca
     3361 ggagaaattt ttattatatt gcccccatta attctttttg aaggttttaa cagagcttcc
     3421 caatgattgt ttcctaaatc cttaagcaaa aaaacttcga catttgcacc ggttagtttt
     3481 tgtccaaaaa gccttgcggg gataactttt gtattattta aaactaaaac atcattttcg
     3541 tctaaaaaat caacaatatc aaaaaaatgt ttgtgggcga ttttttttga atttttttca
     3601 agcaccatca tccttgattc acagcgtttt tcacatggaa gctgcgcaat cagttcttca
     3661 ggtaaattat aatcaaaatc agaggttaac atatctttcc tctcgcgtca caacccttaa
     3721 gggtgaagaa tctgagatgc ttcgctgaca ctcagcatga cgtgtatttt tttattcttt
     3781 aggttcaatt tttttggcag tttttaccgc atcgtcgtcg attttttgtt ttttcttttc
     3841 atcttcaatc tcaagctgtt tgtttattac aacagtctgt atgacctgaa ttacgttgct
     3901 tgagatgaga taaagcaaaa cacctgcagg aatcggaata aagacaaaag ttgccataat
     3961 cataatgggc ataaatgcac ccatagattt ttgaattgct tgctgagtag ggtcttgatt
     4021 ttgtgaaccg gcagatgcca tcatgatttt ttgggttgca accattgtaa gtccgaataa
     4081 tacaatcaaa agcaagacat cataatgtag agaacttttt acctcttttg tcggcattga
     4141 gtaaataaga agtccgtttt ttctggcaaa ttcaaccttt tcgctttctc tgttttgtgc
     4201 atttgtgatt gttaattccg ccttttggat ttgatcaagc tggctgaatt ttaagtcaag
     4261 actgtctaag ctgactttga aatctatagg ctgacccgga tttatttcgc cttgaacttc
     4321 aagtgctttg tttggttttt ctatttttac gttttcgata gtttcttttg gaagataaac
     4381 tttggcggtc ttgccaagat aaaatgtatc gtattttgaa acgttcattg ttccgtcaaa
     4441 agaaactccg gcagaagttt tgagtgtagc atcaagccgt tttatgaaca aaaattgcgt
     4501 gtcgccggct atctgtataa actgcgggct catcaatgca gaatagagca aaatgaatat
     4561 aggcatttga agcaagagcg gaaaacatcc cgacatagga ttaaactgat gttctttgta
     4621 gaattccatc attttgcgct gcattgtctg cgggtcgctt ttatacctgt cttgaatagc
     4681 tttcaatttt ggctgaagcg tctgcatagt cttcattgaa cgctgctgag aaacactcag
     4741 cggccacata aaaagcctta caacaattgt taaaaggata attcctattc cgtaatttcc
     4801 tactatattt gataaagccc ttaatgcttc tattgttaaa gttacaaaat ccatattttc
     4861 taattcctct gatattttat ttattattca atgctaacac aaacctgtga gcaaagcaaa
     4921 gtaacaaagt gtaaatttca aaatcatcaa acgcttacag tgttgcaaat aaaggttata
     4981 gcaagcttga caaagcatta ttttaaatat aaacttgtag ttgcctggca aatatatcaa
     5041 acttgtcggg atgtggcaag gaccaatgga gaatgccaca aaaagaaagc aactaaatgt
     5101 tgttttcgct cgccgcttga agggagctct tggtagcgcg aagccctacc aagctgaaca
     5161 aaatgacaat taaatattac atcgggatgt agcgcagttt ggtagcgcac ctcgcttggg
     5221 acgagggggt cgcacgttca aatcgtgtca tcccgaccat ttaaagactc cgtaatgggg
     5281 tcttttttca tggatgaccc ctcttgctgc gctcgcatta aacggctccg ttcgctcctt
     5341 taacgaaaac ttaatcgttt ccgtttcgga gctcaactcc gcaaaataca aagtattttg
     5401 cttgcctctg ctcgcttgcg ctacatcgtg gcaacacgcg gttttttagg ctcattcttc
     5461 gccaaaaacc agctggttgc attcgcttcc ctaaggctca caagttcgcc aagggtaagc
     5521 ctatcatccc gaccatttaa agactccgta atggggtctt ttttcatgga tgacccctct
     5581 tgctgcgctc gcattaaacg gctccgttcg ctcctttaac gaaaacttaa tcgtttccgt
     5641 ttcggagctc aactccgcaa aatacaaagt attttgcttg cctctgctcg cttgcgctac
     5701 atcgtggcaa cacgcggttt tttaggctca ttcttcgcca aaaaccagct ggttgcattc
     5761 gcttccctaa ggctcacaag ttcgccaagg gtaagcctat catcccgacc atttaaagac
     5821 tccgtaatgg ggtctttttt catggatgac ccgattcttt aaattttatg caattttttt
     5881 tgggcttttg ttttagaata tttaaaggtg tattttttaa ggcaaaatta tgataaatat
     5941 tccaaaaatt aatatattaa gggcggtaac acaaaccgat gttaaaattg agctaaaatc
     6001 gcgcagaaat atgaaaaaat atttcccgaa ttttcacaaa ctctgcatag aagaaggctt
     6061 atatacaaaa gcagggttta aaatcaagga atattttaga caaagaacaa aacaattttc
     6121 aaaaatgttc tcagagcaag agcatagaga tcgtattgac gggtgttttt caaaaaagta
     6181 aaaattattt taccacttca ataaaatcat aatcttccaa gtaagcaaga tgttgggcag
     6241 agattaattc tcccggaagc aaaattgaaa tgcccggagg acattctgct atgacttcgg
     6301 cacaaattct tcctacagaa ttttcacggc ttacagtttc tttttgagaa taaaaagctt
     6361 cttgcatgct catttttatt ttaggagtgg taaggggcat gtgtttttta ttttccatat
     6421 aacaaatatc tgtgtaattt gtatttgaaa ttttttcaag acaatcggca aggtattcaa
     6481 aatcagagcg tttgtttccg atatttgata aaattaaaat tcctatgtca gatgccgatt
     6541 ctacttcaat tttaaaatct atttccaaga ttgattcaag cctttttccc gaaagcccgt
     6601 ctgcttttat aaaaaccttt gtaacatcgg ttttatatcc gaaatccgat ttaagctggt
     6661 gtatatttgg cagtgagtct attcttgttc ttaaatattt tgcgttattt accgctcttg
     6721 cgatttgttt tttgcccaaa gggctttgaa ggttagccct tgcagcatca aggcttgcaa
     6781 gaagtatcag ggaaggactt gttgtgtgta aaagtttaag tgctttttct atttttgaat
     6841 catctattat agaatttttt cctatatgaa gcattgagct ttggctcatg cttccgcctg
     6901 ttttgtgaag cgactgaact acaacatctg cgcctgtttc taatgaggaa ctgggcaaat
     6961 gtttattgaa tttccacaaa cttccatgcg cttcatcaac gattaagggc acattgtatt
     7021 ttttgcagat taaacttatt gattttatat cagaaacaac tccctcataa gtcgggtttg
     7081 taatccaaac aagtgaaaca tcttgatttt cttttaataa attttcaacc gattcgggcg
     7141 aaacacttcc ccaaattccc cactcgtcaa gccgttgagg aataagccat aaaggctctg
     7201 cgcctgatat tatcatgccg gtcaaaagcg aacgatggca gtttctgttg gtaataattt
     7261 tttgcccttt tttggtaagt cccattgcta tggcaagatt tcccgctgta gagccgttta
     7321 aaagaaaaaa ggttttcttt gctccaaagg cttgagctgc aagttcttgg gcttctttaa
     7381 taggacctgt cgcaggatga agtgtcccta aattgtcaaa ctcgtctgtt gtatcgatat
     7441 ttagtgcctt ctgtccgatt aactttttaa attcaggata cgttcccttg ccttttgtgt
     7501 ggccggggat atgaaactgg taggtaggat ttttaaccgc agcctcaagt gcttctacta
     7561 taggtgcttt aattttggtt tcacaacggc ttcgggcatg tatattcaat tcaaaattcc
     7621 ttctaaagtt tagtttttcc aataataaga tactacaaaa aaagtatttt atatacaaac
     7681 aaattgatta taatgttaaa tactgaacag gtagctggaa ataatttttt gaaaaaaaat
     7741 aatatattaa aacttttaat attgttttgt tctcttatct gtattaattc ggtttatgca
     7801 gatgaaacca ttgacagtgc caaaacggaa aaaacgtcta aaatagattt aaaggcggtt
     7861 atttttgatc ctgaatttga caataaagaa gaaataaaag ttgaagaact cagtgacgct
     7921 gataagatta aatacaattt acacgaagca cttcacgggg aagtaaaagc cataagcaca
     7981 aaagggcttt ttgcagacaa aatgacggta aaatttgacg agggtccgat taaaagcata
     8041 gttccatgga ttgattataa cggttcattt tcaaatgtgt ggacagggga taattataca
     8101 aatactttct acggcataaa ttttgccgat gtagggttta acataaacat gcgtgatgac
     8161 aaaacttttg taagagtcat gttcagccct gtaaggtatg ttgaaggaaa tacttatttc
     8221 caaagctttt ttgcagacaa ttatataaca aggaaaataa gtaaaaataa tactttactg
     8281 gtaggtcata cttgggtgcc tctaggtata gaaggaaaag gttcgcctct tattttggac
     8341 tttttcaccc gttctcagac ttcaagaaca tattcttctg tcagggctct tggaacaaaa
     8401 ttgatgggca attataaata tgctgattat caggtgggct tttacagctc cggaagattt
     8461 tttaaagatt ttttccctgg gcctgaagtt gctgcttggg ttgattttaa accgcttgcc
     8521 aatacaaaag gcgaatacgg aaaacttacc gtaagcggtg gatttgatgg cggtaacgct
     8581 cagagcaatt atgccgtagg aacagctgcc gttaattatg aatataaaag attgagactt
     8641 acaacagaaa tgggtatagc agacggttca aacggtgcaa caggatttac cagcaataaa
     8701 tcgggcggag ttaatacaac cttgacttac agagtgactc caaaaactca ggtgcttttc
     8761 aggtttgacc aatttgaccc caacaccgaa aaagcaaatg atgttagaaa agaatatacc
     8821 gcagggatta actattttat aaaagatgaa gcacttaggc tcattatgaa ctttacgcac
     8881 tatgaaattg aaaacggtgc ttttggcgac agaattatgg taggcactca aataatactt
     8941 taattttaag ctaagatttt tattcatact gataaaacaa aaatattacc cgctattttt
     9001 aaattcaatc tgatttaata atactttttg gcgtttacta taataattaa ataaacacca
     9061 tatttattcg gggaggaaaa tatgaacacc ggtcaggtaa aagtattaat agtagaggat
     9121 cataagctta tgcgcgtggg cttaaaatct ttatttgaag attttcccga aataagcatt
     9181 gtggcagaag ccgaatccgg caaagaagct attgaaaaga taaggcttac aaagcctgat
     9241 gttgttttaa tggacatagg tttgcctgat atgacaggaa ttgaagccac taaaaaaatt
     9301 cttgaacagt ttgagggggt aagggttatt atgctcactt ctcacacgac agagcaggaa
     9361 gtcttggatt ctttatctgc cggtgctaac gcttatgtac taaaagacat aaatacagaa
     9421 attttaatga tgattataaa aacggtaaaa gaaggtgcca tgtggcttga cccgcaaatt
     9481 gtgcctataa tcagggaaaa aaattgcgga gtaattcctc aaaaacaaat gtccagagca
     9541 aattttaaag cgcagcattc aaatttgacc gaaagagaat atgaagtctt aaaacttgtc
     9601 gttgacggaa aatccaacca tgacatagca aaagagcttg tcataagcga acacaccgca
     9661 aaggctcatg tttgcaatat tattcaaaaa ttggttgtag atgacaggac acaggctgcc
     9721 gtaaaagcac ttaaagaggg cttggtttaa gccaaatatt tttgaattgt cgatttgtca
     9781 aaaacttcta tgctgtctgt ggaatgaata aatatcatac agtttataag cgatttaaac
     9841 atttcaaatt cgccaaaagc cgtttttttg cgaagctcgt ccatattgtt tgcaagaaat
     9901 tctgttataa caattttttc tttaaacaca agactggaga aataatcaaa cgcttctttt
     9961 gttttaaccc cttcaagata aattatatcg ggtgactgaa attcaataaa tcttagggct
    10021 ttgtccaaat taaagccgat attttcgttc atttcgcact ggttgacgtt ttccaaattg
    10081 tattttgcaa tggattcaat cgtcataata tttttaccgc tccctgcaat ttccaacaga
    10141 agcgaataaa tcatgtgggt ttttccgcta agggcagagc cgcacacaag aacaattccc
    10201 ggctcattaa gagagttttt tataatattt aagccccctt cgtctgtaat tatttttctc
    10261 aggtttttcg gaggtttata aatttttaaa gagattcttt ctcccaaaat ggtaggaaac
    10321 gcagaaatac ttgcaattaa ggtcatatca tcgactttaa agcataattt tcctaactgc
    10381 ggaacttcac ttacagcagg gtctaaattt gaaagggttt taagcttaga aacaaatgaa
    10441 gaggttaaaa cagagggtat atagcctttg tcaaaaactt ctgaattttg cttaaaatta
    10501 acacctaaac cttcttttgt atattcaaaa taaacttcgt gaacattttg aaaaattgct
    10561 tgttttaaaa tcgcacggat tattttttgg atgtgttcat cgcttaaatc ttcgctgtga
    10621 agttcttctc tccagtcgta gtttatgttt tcgctgtccg taaaaatttc tccaacggca
    10681 ttttctgttt tgtaatactc atcaattttt tttaagatac ttatctcgga agagattaca
    10741 ggctcaatag agcagccggt tgtttcgaca actttatcta ttgcaaacaa atccagaggg
    10801 tctgccatgg caatagttaa tacatcttca attttaaaaa gagggattat tttatatttt
    10861 tgcgcatcgg aaaaatttat aaatttcagg caatttttat ccaaagcgta atcttcaaga
    10921 ttgacataag ggatatgaag ctttgcttca aggaatttaa gcagtttttc ttcttccaaa
    10981 atacctgaat tgataagtgc ctgtccgaca tttatgtttt gggcgtttgc aagttcctgc
    11041 gctttttcga ccgtatcata atcaaccaga ccttctctga caaggtcgta tttaagtttt
    11101 tctaaagttt tgtttgtaac aagttgctcc atttatccac ttctatttac tatttactat
    11161 ttaccattta ctatttacta atttacccct ttcccaaata tttttctacc ttcttaacga
    11221 tttcttccag ttcaaaaggt tttgtgatgt attcgtcaac gcctgtttcc tcgcctataa
    11281 gtttatcttc atcctgagag cgtgctgtaa ccattagaat aggaatattt ttgtacttac
    11341 tgtcatattt taaaagcctg caaattttgt agccgttaat ttttggcatc ataacatcaa
    11401 gaataatcaa atccggcaaa acatcttttg ccatttgaag cccttcctcg ccgttatatg
    11461 cgcaatagca tttaaaacct gccgcttcaa gcacaaattt aaggctttcc actatatcct
    11521 gttcatcatc aactatcaaa atctttttca cgttttactc ctgtttaata tttattttgg
    11581 attgcttcgg aattacgctt tcgcaatgac ggaaacaacc cttcagccgt tcagcttttc
    11641 accctttcag ctaggttaca ccccttagct tttgaagagt ttcgtcaact tttttcatta
    11701 attcccactc atccgccccg tcattgggat aagttgcgta tgaatacatt atagcacatt
    11761 tcgggaacaa attttcatgg tctttaagat aggtttggat tttctttttc aaaaaatcgg
    11821 cagagaattt gtcgccgtcg ggaattacca ttgttaccgt tttgttttta ctttcggttt
    11881 cttgaattga actttttaga taatttttgt taattaaatg tttatcttca agaagatttg
    11941 ttattaaatc cttttcgccc tgcattttaa caagtgtcaa aacagaattt gaaacttttg
    12001 ttttttgtat cgcctgctta aattcaagca taaaattatt tatttcattt gctacaggaa
    12061 ttgcaaagta aaaagacgaa cctttttcgg gtttgctgtt gcaccaaaca gccccgttgt
    12121 gagcatcaag aagttgtctt gcaataggaa gtccgagtcc tgagccgccc actttgcgcg
    12181 aaagagagtt ttctatttgc gcgaacttat cgaaaacatg cgctaaatcc ttgtatgcaa
    12241 ttccgatacc gttatcttta acccttacta ctatatattt acccttaagt tttttcaatt
    12301 cttcctcaaa gccttcttga atttgtattt cttgcgcatc aacgacctgc gagcatactt
    12361 ctattttgcc gccttcgttt gtgaatttaa tcgcgtttga aacaaggttt gtaagcactt
    12421 gttcaagcct gtgggcatca gcgtatattt ttgttttgtt gtcttcgatt cttgatacta
    12481 taacaagccc tttttctttt gccatttctt ccaggctggc ctttacatag tcaattacag
    12541 gctcaatggt ggtaagcgca aatttgaaat ccattttgcc cgcttcgatt ttggacaaat
    12601 ccaaaaggtc gtttataatc ccagaaagcc ttgtcacgtt tcgctttgcc atgtttataa
    12661 atttgtccat ggtttctgtt aaaattcccg attttccgct taaaagaatg tcgagtgaat
    12721 tttttatcgc agtcaaaggc gttcttaact cgtgcgatac aatcgaaata aattccgatt
    12781 taagccgttc taatctttcc aatttctggt ttgttttttt aatttcttca tataatgatg
    12841 cgctttttaa aggcagcgaa acctgctgga caatggtttg aaaacaggtt gtgtcttctg
    12901 tggtaaaatc gttttctctg taaatttcaa tgaatccgaa aaacttttcg ctcaaattta
    12961 tcggcgcaaa aatattgtca tagcgaagaa ccgaaaaatc atattctttc atagtgtgct
    13021 taaaatgttt ttctattttt aaattttcga tatttaattc aaagggcatt tctttttttt
    13081 caaaaagaga tttgtaattt aaaattgtgc gaagttttat cgcttcaaga agtctgtctg
    13141 aaatttgata aagtgaatta attatcaaaa caggctcgtt ttcactgcga aaagttaaaa
    13201 cgcaggcaag cccgaaactt aaacttttat ctaaaccttc gaccataaaa tcaatgagct
    13261 tgtttttatc taaagttcct gcaaactgcg aacttgtatt gtataaaacg ttaagctgat
    13321 aaaggcttct ggcaagctcc ctgtttgaat cggaaagcat ttcaagcgag ttttttgttt
    13381 taatgtttgc gtttatggtt gcaagcaaaa agtcttttgc gacaggttct tttaaaaatg
    13441 cgctcgagat tttaagcaat tcagggtcga tgtcatcggt ttttgtaacc aaaatggttt
    13501 gaagattgtc gttaaatgct ttaatttttt tgtgggcgga ttttaagtca aaagatttta
    13561 cttcggcatc aagaataaca aggtcagggt tttggagctt tgttttttca accgcctcgt
    13621 ttatggtaaa tgcacacaaa aaatcgtgcg gatttttgtc tatcatatca ctaaacagtt
    13681 tgcacttttt gtgattgtcg gttaaaaata aaatttttgc caaagccaat ctcctcttat
    13741 aaacatttta gcaataaatt ccaactaagc gagctttttg caaaagttaa caaagaaaag
    13801 ctgtgttttc ttgattttaa aatgctgttg tgtttaaata tacttgaaac gcgctttcct
    13861 cttagctcgt ttttgaaaaa agccaagcgg cgggcttttt ggattttaca cttaataaaa
    13921 ggaaaagaaa aacaatgtta aaaatcagac ttaaaagatt aggcgcgaaa aaaacacctg
    13981 cgtttagaat tatcgttata aattcaacaa caaaacgtga aggaagaccg attcaagaat
    14041 tagggcatta taaccctaaa acaaaagtga tgaaacttga caaaacatca gcactcgaat
    14101 gggttaaaaa aggcgcacaa cctacagaaa cagttcaata tttaattgac aactgcaaca
    14161 ctgacggttc tttgaactat aagaaaaaaa ccgaaaagaa actttctaaa aaagctatag
    14221 ctaaattgga agccgaaaaa gaagcaaaag ctaaagcaaa agaagaagaa aaagcggcaa
    14281 aagaagctcc taaagcgcaa gaacctgctc aagagcccgt tgtagaagaa gcaaaagttg
    14341 aagcggctga agaaccaaaa gcagtcgagg aaactcctgt tgaagctgta gttgaagaag
    14401 ttgcagttga agaagctccc gtaaaggaag aagcagctgt tgaaacagtt gttgaatctg
    14461 cggctgaaga agttgttgaa gaaacaaccg aagcttaata aattaacttt tataaaatag
    14521 gcgaagtttt cgaagaaaac ttcgcctatt tattttgact gttttttgtg acgatttgtc
    14581 aactgttttt ataaaaaaga tttacttttt ttgtaatttg atttaatttg aatttaacac
    14641 aggagttttt agccatgaat gtacctaaaa tgggtcatgt aagttttgga aatgcttatt
    14701 taattcaaac gcgcaatcct aaagtgcccg aggcattttt aatgatagat acaaatttga
    14761 gagcacaaga taatcctgca ctcagatggg aacacatttt gacctctaaa acagaccaat
    14821 atgtaaaagg cggactgctt ttaaccggtg acgaaatgct tgaatatcac aggcttgaaa
    14881 aaaaatttaa atcaggcagg ggagaaatta aagaaaaaga tttttaccat aaattaacca
    14941 aaatggtaaa caaattcaaa gagattgcac aaaaaatccg ttttgactct gatgttgatt
    15001 ctctaaccaa acaaatccct gaagtttcaa ctatagcaaa agatttgaaa aaatttattt
    15061 aaactgtcct cttagacacc cgttgagccg aagcctcctg cggcgcgcga agtttcacta
    15121 agagaaggta caacttcgat ttttgctttt tcgacacggg cgattatcat ctgcgctatg
    15181 cgctcgttcg gtaaaattgt gtattcttcg gtcgaaagat ttattacagg aacacaaacc
    15241 tctcctctat aatcctcatc tattgtgcct acacaattca ccaaagagat gccgtgtttg
    15301 atagaaattc ccgaacgcgg tcttatttgg gcttcaaagc catgctcaag ttcgatttta
    15361 atccctgtgg ggattaattt tcgctcaaaa ggtttgagaa ggacagggtt ttcaattgcc
    15421 gcgcacaaat ccatgccagc agcaccttcg gtttgatatt cgggcaagta agtgttatga
    15481 gctaattttt caatttttaa tgttgttgtg tacataatcc aattttagca aaaaaattct
    15541 taatcataat ccaatgtttc gatatgttta atatcatctg aactgcccca attttgttcg
    15601 taatttttca ttttaacaaa tttttcaaac gaagaaaact cccaatcttt gacatttttt
    15661 gtgtgattat gttttacggg attgtagtga atataatcaa tatgtttgtt taaatcttct
    15721 tcgtctcgga ttgtgtgttc ataatatctt cgttgccaaa tacctctttc acctttgttt
    15781 ttgtacccgt aggttggggt ttctacccca acatcaatat tatttgagaa ataatatttt
    15841 attgaagtga ttatcttcgg gtattcgtga atattttcgg gttttaaaat catgtgaata
    15901 tggtcaggca aaatacaaat tgcaaaaatg tcgaaattaa aaaattgttt tgcattttta
    15961 aatgaattac gcaataaatc aatgttttta attaacattg gttggcgttt gtatgtgact
    16021 ataaccaaat gtatataagt atttggaata aaaagtcttt tatatcgcat gctttaatta
    16081 tattgcaaaa ttatcagaaa tgttggggta gaaaccccaa cctacttctt ttaaaccaaa
    16141 ccatctctaa tagctttaac agctgcctga accctatctt gtacagataa tttttgtaaa
    16201 atagagcaaa catgagtttt tgcttcagtt atagttatat caaatatttt tgcaatttct
    16261 gtattgcttt taccaaatac aatttgttct aaaatttctt cttctctgtc agttaatgag
    16321 tttttgtagg ttggggtttc taccccaaca ataccgacat ttccgatacc gccttgggcg
    16381 aattggggtc tgccgccgcc tttaccacct aaaagttgcg agatattaga gacaatttga
    16441 ccggcattta ttccatttgc cacaaattta tccgaaactt ttgcaacgat tgttgagtct
    16501 gacataatta ctataatcga atcacccaat ctttgagcta aatattcaac cccttctttc
    16561 agtgcgtttg aatccatagc gtcaattttt gaaataaata attttccgcc ttcaatatct
    16621 tctgccttat ccacatacga agcaaatttt tcttttgcca ttgcgttttg aacacttgcc
    16681 aattcctttt gaagcgattt gttttcttcc tgaatttttt caatccgctc aaacacttct
    16741 tcgtaaggcg ttttaagatg acctgcgatt ttatcaatca catcttcttt gtcgttaagg
    16801 tattctaacg cggtttttcc cgctaccgct tcaattcttc ttgtgcctgc tgcgattgcg
    16861 ctttctgaaa caattttcat caaacggagt ttgccgatgt tatcaatatg tgtcccccca
    16921 cagagttctt ttgagattgt tccgatactg acaaccctta cgtcattttc gtatttttcg
    16981 ccaaatagtg ctgttgcacc cgatttttga gcatcttcaa gtttcatgac ttttgtcgaa
    17041 acttccaaat tttgcccaat ccaatcgttt attatttttt caatccgttt gagttcgtta
    17101 taacttacct gacgcggata cgaaaagtca aaacgtgtgc ggttttcttc cacttgtgaa
    17161 cctgcttgct taacctcatt ccctaaaact ttttttaacg ccgcttgaag taagtgtgcg
    17221 tttgtgtggt gaattttaat ttcttcacgc cttgcagaat caatttttgc atttaccata
    17281 tcgccgattt ttacttcgcc ttcgataatt tcacagcgat taacaaaaag atgatttacc
    17341 ttgaaagtag tcaaaacgct tactttgcaa ttactgtttt ccagaacacc tgtatctccc
    17401 acctgtccgc ctgattcagc gtagaaaggt gttttgtcca aaataatatc aacaaaatta
    17461 tcaccttcaa ctttggcaat aatttttgct tcgatttctt ctttttcgta acctaaaaac
    17521 tcggttgagc cgaactgttt ttctatttca acgtatttca aatcatcggt cagaacgact
    17581 ttttgagcag ccgcttttgc ccgttttttc tgttcttcca tttctttttc aaactctttc
    17641 aaatcaactt taaacccgtt ttcttttgct atctcaacgg tcaattcaag cggaaaaccg
    17701 aaagtatcat acaatttaaa agcatcttcg cctgaaatag cacatttacc cccttgcttt
    17761 ttggaaagca attcttccaa aagtttatag cctctgtcta atgttttatt aaactgttct
    17821 tcttcgtttt tgattgtatt tttaattttt tctgcatttt tgatgaggtc agggtaagca
    17881 ccgccgtaat ttttaaccac cacatcgaca agcttataca aaaacggaag ttccaatccc
    17941 aacatttttc cgtgcctaag ggcacggcgc aaaatcattc tcaaaacgta atttctgcct
    18001 tcgttgccgg ggattatccc gtcatttatc aaaaaagtaa cgcagcgggc atgatcagtt
    18061 ataattctga gcgaaacatc agttttgtcg ctttgtttgt atttaacgtt tgacatcttg
    18121 caaatttcat ctagaattgg attcaataaa tcagtttcaa aggttgaggt tttgccctga
    18181 caaaccatag taattcgctc cagccccatg cctgtatcga cgtttttctt ttcaaggggc
    18241 gaaagtttgc catcttcatc ctggaacatt tccataaaaa caagattcca aatttcaacc
    18301 cagcggtcgc attcgcaggt tgcaatgcca caatcaggcg aacatttgaa ttcttcgccc
    18361 aagtcatagt gaatttcaga gcatggcccg cagggacctg tcggccccgg agggccccaa
    18421 aaattatctt ttttgccttt gcggataacc cgctcaggcg aaagtccgac aacttttgtc
    18481 caaatttcaa aagcttcatc gtcgtcttta taaattgtta cccaaagttt ttctttatcg
    18541 agtttaaggt aatttgtcac aaaatcccaa ctccactgga tgatttcttt tttaaaataa
    18601 tcgccgaaag aaaaattgcc cagcatttca aaaaaagtgt ggtggcgggg ggttcttccg
    18661 acgttttcta tatcagaatc cttaccgcct gctctggcgc atttttggca cgaagtcgca
    18721 cgggggaatt ttggtttttc aagccccaga aaataaggga caaactgcag catacccgca
    18781 gttgtcaaaa gcacggtcgg attgtcaggg acaagacttg aactttccac caccgtgtga
    18841 ttatgctttt ctttgaaaaa ttttaaaaat tgttctctta tttcattgcc cgatatcata
    18901 tttcctcgct atttgttttt agtttaaaag gtgaaaacat gaaaagttta attttcttat
    18961 tcacttaatc acatagtcac ctattcacct taaaaaaatt atatattaaa aacaggcgaa
    19021 tttacatttt ttatcaaaaa atgtaaattc gccttgcctg tagaaattta tatggtagga
    19081 ttggagtata aattaaataa aataagtaga aacagtaaaa taaagacagc gtttttctta
    19141 acataaaacc aaaaaacaag aattgtaatt ttttgttaaa agcgtgacag aatgataaaa
    19201 gtctttttgc ttactttttc tacagaaagc gcgagtgagc agagggcgag agcccgttta
    19261 atgcgagcga gcaagatagt aagccctggt agctcagctg gatagagcaa tcgccttcta
    19321 agcgataggt catgagttcg aatctcctcc agggtaaatt aaaaaagaag gcgtaagcct
    19381 tcttttttcg tgtttatttt gttttttaca ttaaaacaaa cttacacatc taaatagtat
    19441 aactaactct ttgaaaattt tgtgaaaaca aaaattcaaa attaattaaa tttcataact
    19501 ctattcatct aaatatgttt gtactaaaat aaaaatatta cgcccccgta gctcagatgg
    19561 atagagcgat agcgtcctaa gctgtgcgtc gttggttcga atccaatcgg gggtattttt
    19621 catgatttta ttttatattt atcaaatcaa ttactacccc cgaggatacg aaccaggttt
    19681 aatttttcgg ctcctgccta aaaatcaaac aggttaatcg ataaatcgat accctcatct
    19741 gccaggctca aacttcctgt tttcgcctgc atctgagctg aaatccaatc gggggtattt
    19801 tgttttaatt tgaacggtta tagtaaggaa cgttattcga accgcaatgt ttctcctgtc
    19861 ggtcgaaaca ttgttggtta atcgtcccga tatcctgcct cgtacggctc gagctcctcg
    19921 cctacgattc aagagaaccc aatcgggggt atttttttat tgatttattt tacatttttc
    19981 aacttcacta gcaaaatttt ttctttgtcg gttttataaa attgcaactg tcagtaaatt
    20041 tggaaaatta tgataatttc tgttaactta tttaattaca atcaaaatgg ctttaaaatc
    20101 aacaaaagcc ctgttttttt tggtgcaaat ttagtaagcc caaaagaagt tcaaaatttt
    20161 ataagactta tttcaaaaca gaagcttcaa gaaaacatgt tggttttgac ttcccccgaa
    20221 actcaaggca gggcaatagg tacacaaggt attgaaattg caagtcaatt tattgaagct
    20281 caatttgaaa aaatagggct tgagcctttt tgtcaaatcg gtttaaaaac tttttctcaa
    20341 ccatttttgg cttatcagcc aaaattatta ttcgaagaaa atttaaaaaa catttacggt
    20401 tggattcgcc acaaaagcaa tccacctgaa attccggcta aaaatattgt tggaattatt
    20461 cgtgggaaaa atcctgaaaa atatcttatg ttgactgccc attatgacca cttggggagt
    20521 attccagaaa aaaatcttat attccccggt gctaatgata atgcttccgg agtaagtgtg
    20581 ttattggaac ttgccagaat tttcaaaaag attaaacagc ctgaaaagtc aattattttt
    20641 gcagcacttt cagatgaaga aggttataaa aaaggtgctc aaaccttggc gcaaagtttg
    20701 attgataatg aaatccatag tattgatatt ataaatatgg atatgctcgg cggacaaaag
    20761 ggaaatacta ttgatctttg gcaagaaaac aaaaaattat ccgttaatat agtcggcgca
    20821 tttaaacaaa catgcgatac cttagggtta aaactcaggc tgcacagagg agatccttgt
    20881 tcagacgcta caatgtttaa tattgaagga atgcctgtcg tatgtgcttc ttgggatttt
    20941 ttatctaaag taaatactcg attttttcac accaatgccg atactgttga caaaataaat
    21001 ccggagattc ttgaaaaagc agccaaagca ataggtacag cttctttaaa tctgatttgt
    21061 taagcctttt aattgtgggt ttaaagaaaa actttaaaaa taaaaatttt ttgcaaattt
    21121 tgtgatttat tcgctgctgt ctttttcttc tttttcataa caatttgtaa cccagtggat
    21181 tgcaagggca ggtactaaaa acagaaatat aagaattgtt tttattaatc ccacaaatat
    21241 tacccacaaa ttttgataag ttattacatc aaccccgaaa gcactttggt aaatgcttgc
    21301 aacgtaacat ttgcaagggt aataaatcac cgcagctaaa ataagaaaaa acagtccgac
    21361 tacaaaacat ttataaaaaa tttttcttaa tacaataagt tgctctttta tttccatata
    21421 aaagccctcc ttaagatatt ttcaacaaaa aaaatctttt ttaaattctc ttttcgggta
    21481 attattaatt tttaatcttt atttttcata tgagaatgca ttgcctcagc aaaaagtctt
    21541 aatccgaaat aagctcccaa gccaagtgca atagctattc ccgacactcc aaacattgtg
    21601 gaaattaaaa ccccgcccaa aataaatgca aaagcacctg ccgcagatgc cgaaggagtt
    21661 ttgtcgctaa aatcatcttt tgatgcttta ttttcaggct tttccgtttt tataaactca
    21721 tctgttttta tattattttg taaagctttt gctgcgtttt gtctaaattt tatcggctct
    21781 gtatgttgaa tttgaacggc tttggactta atttcaaaaa tatctctgtc aagcttttgg
    21841 tctataacaa tcctgtcttg aaatcttcta tccataccgc ttctgcgctc cacatcaaca
    21901 ggcatgttca tctgccttct gtcgcttgta aatcgatatt gaggatataa ttcacgtttg
    21961 gattctatca taaatttttc cttattcttt cattataaaa ccacaacaaa taaataattg
    22021 ctattttagt ctttctaatt gaataaatct caatttactg ttaaaatgca tttatgaaaa
    22081 aaattatttt gtttttatgt ttgttaatat ctgcgccagt actatcacaa gaggcagaag
    22141 gttttgccgc agattctcag ggtgcttcct tgcaggataa acctgattac aacaaatctg
    22201 ttgataaaga agtccaaggc tctgatgatt tttttgatga acttatatat ccgcaatacg
    22261 gttcaagcca aagcaatgat tttgaaggca gacaagacca aaatccgaca gaatcaaatt
    22321 acgggtatga cggatcatag taaaaggaga aatttatgca tgtaatagca atcaatggca
    22381 gtcctaagtc tgaaggaaat acttataatg cgcttaagct tgttttggaa gaacttaaaa
    22441 atcaagggat atccggagaa attatacata taggaaacaa aaatatccgc ggatgtcttg
    22501 catgtggggg gtgctttaaa aataaagacg agagctgtgt tataaaagat gaagtaaatg
    22561 aatgtattca aaaaatgaag gaagctgatg gtatattaat cggctctcct gtttattatg
    22621 cttcgattgc gggaacttta aaatgttttc ttgacagaac tttttatgtt tgctccgcaa
    22681 acggaggact tttaaggcac aaagtaggtg caagtgttgt tgtagcaaga cgttcgggag
    22741 aagtcgcagc ttttgaccat ttaaaccatt actttacgat agcagaaatg tttatgcctt
    22801 catcaaatta ctggaacgtt atccacggaa ggatgccggg agaagttttg cttgatgaag
    22861 aaggggtgca gataatgaga gttttaggca agaatatggc atacctttta aaacaactta
    22921 aaacaggaac acagacagca cccgagcaag aacaaaaaat gcttacaaat tttatcagat
    22981 aagattgagt cattgtatga aaagatttaa aaaaatattt atattgttat tattttttct
    23041 ccttaatatc tgcaattatg ctttttcaca aaataattat tcaaatgccg caattcaaat
    23101 ttataactca ggcgtgaatt ttcacaaaca gcataatttt gatttggcag aacaaaaata
    23161 ttttcaagtc ttaaagattc agccgaattt tattgaagct aaaaataatt tggctttact
    23221 ttactacaat aaagcagtga acaacttatc ttcgtccaat tatactgatt ctattgtgta
    23281 tgcccaaaaa gcactctcat atggctataa gcctcttgaa tgctataata ttattgcaaa
    23341 ctgttatatg caaatgaaag attatgaaaa tcttatttta attggaaata aattagatac
    23401 gctttctcca aatgacgaat ttatccttaa ttatctggca cttgcatata tgaatacaaa
    23461 tcaaaatgaa aaagctcgag atatttatca aaaaatatta ttgattaatc ctgatgataa
    23521 ggctgcacaa caaaatataa aatatatcaa ctattgtcat agtcagaaag ttttaaataa
    23581 ttctataaat aatttaccga cagtggctca tgccccggct tctttgtaca ggctgattaa
    23641 gccttctgca ggaattactc agggcaccgt tgataaaatg aaatatattt tggatttaat
    23701 ttggagcgaa ccaaacggtc aaatactttt acaagaattt ttaaataaaa aaattccgat
    23761 aaagatatct caaggaaccg taaccgcaaa tgcgatgttg caaaacaaaa aaaacacgct
    23821 tttactttac ggatttattc ctgttttttc ttatgataca tcttcaatct ctgttaatat
    23881 tgcatttaat tacatttcgg atttttttaa tccaaacatt gactccggac atagaattta
    23941 tgacttacaa gtttttgtcc atgaatttgg tcatgctttt atgttatgca aaaattcagg
    24001 gcacagagat tctattgaag aagaattagg cgtttcaatg attggtttta attctgctta
    24061 caagataatc attggcaaag gtttgactaa agaacaaacc gaagaatata gtttgaatgt
    24121 tttacaatct ctgttatcgg acgaacatcg taatttaccg gtttataatg gttttgtcag
    24181 tgaaatacaa aggtatggta tcgttatgcc ttatcctgaa atttattcaa acctccccgc
    24241 aatgtataaa aaattgcttt gtgaagaaaa aatttcgcct gtccctaatt tttacgttta
    24301 taacagataa tttgatttca atttgtaaaa tttattttaa atatttccga acatttttta
    24361 accctgactg tgataaaatt actttagaaa tttacttttg cgaggagtcc atttatgaaa
    24421 atagtatttt ttgacataag agaagatgaa acggaggctt ttggtgcttt atgcaaaggg
    24481 gagcgcaaag gaaaatgcca aattgaatgt acatgcaaaa tgcttaaaga aacgcttaac
    24541 gagcaaaccg ttttaacaga tgaaattaaa caagctgaag ttatttcatg ttttactttt
    24601 tcaagagtga cagaaaatgt attgaagcag tttcctaatc ttaaattaat tgcgcttcgc
    24661 tgtgtcgggt ttaatcatat agatgttgaa tattgcagaa aaaacaatat tcaggtttta
    24721 aactctttcg gctacggaaa tataacggtt gcagaatttg catttgcgct tctttttgac
    24781 gtttcaagaa aagttacaag agcatacatt aatctcaaag aaggtgaatt gagcaaagat
    24841 ttttataccg gttttgaact aaacggaaaa acaataggaa taatcggaac aggcgcgata
    24901 ggaactcagg caatccgcat tgcaaaaggt ttcggaatga atgttttggg ctatgaccct
    24961 ttccccaaaa aagaacttgt ggaaaaatat caggtaaaat acgttgaatt ggatgaactt
    25021 ttaaaaaatt ccgatgttgt ttcgcttcat tccccgctta cagaagataa ttttcatctt
    25081 ataaatgaag aaaaaataaa cttgatgaaa tcgactgccg tgcttataaa cacagcaagg
    25141 ggtgaattga tagattcaaa agcactttac ggtgcgcttt ccgagaacag gatattaggt
    25201 gcggggcttg atgttttaga agcagaaaac atgattaccc aaccggatac tattttggat
    25261 tttgattatc tgacagatga ttacataaga caaacgctga ttaacgaaag gcttttgaag
    25321 cttcataatg ttgtggtgac gcctcatata gcatataatt caaaagaggc aaaagaaaga
    25381 atagtttcaa ttactatgga caatattgac aaattttttc aaggtgaaat tcaaaacagt
    25441 gtgttataat ttggcgttaa ggtggaatac aataaaaagc agcaaactcg gagatgaaaa
    25501 atgataaaca aagcaagaag agcggataat attatcgaat ggattattat tggaattgtt
    25561 gcga
//
LOCUS       DAOQAU010000021        25026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_1982_length_25026_cov_6.519883, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000021 DAOQAU010000000
VERSION     DAOQAU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 25026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25026
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_25026_cov_6.519883"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKI94_04615"
     CDS             <1..322
                     /locus_tag="PKI94_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="starch synthase"
                     /protein_id="HNW26055.1"
                     /translation="MFLMPSKFEPCGLSQMIAMRYGTLPIVRTTGGLEDSVVGHPLKH
                     ANGFKFWDYDSTNMLNGIKYAINVFKDKQEWDKLVKSAMNYDYSWKKSAKHYMDVYEG
                     LLKS"
     gene            458..1537
                     /locus_tag="PKI94_04620"
     CDS             458..1537
                     /locus_tag="PKI94_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26056.1"
                     /translation="MSKNLQNQIGKIKKSIVNNNITNNSMATYGKLKEIEHSLYRHGM
                     LSKAGLKDEAKAESAKANSLISNFNSSEDTETLKGYISNTKYVWVASEDSCEACQELD
                     GTEYDSMDDIPDKPHPNCNCYIEEIEMEDDENGNGGGDSNDNGNGGGGGNNRGEPCDC
                     WDKIDEMVEEANALDDELASDMSDIDSIQAEAEGWLVEVQELKAQVEHAQEALEAIEP
                     CGPDCAITGMAVNITDDKELEYTVYNLIKDIEEARVVYEVFQSNKHEMETTANSFDKY
                     YHAKANCESAELGTIETMWAVVFSIFKEVKDFKTKVFSLHMDAQKVFLDCMNDLRADL
                     YGLMKAKEHGYCSDKLKDIDKVFKK"
     gene            1564..2013
                     /locus_tag="PKI94_04625"
     CDS             1564..2013
                     /locus_tag="PKI94_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26057.1"
                     /translation="MKDKIKVVLNKILQVCSIIFKTLACIVLTYFILILPITAIRTGK
                     FDWQIFIIIVVCFLFAFLTNWAILKKGCLRKILMFFVLFLICAVLEIKMLHYLGIESD
                     YCIEDGDCKEGRIVNTEKYGKVLINKENCLKYNWEWYEKQKYCKLWN"
     gene            2016..2486
                     /locus_tag="PKI94_04630"
     CDS             2016..2486
                     /locus_tag="PKI94_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26058.1"
                     /translation="MQKMKVFLTKLFLILFRIIDTVVKIFICLGITIFVGYFAAIASA
                     SAFAGRQGFDWQVLLYVLASVFTILGIWLVAFIKTKKNTKIIYAVIFFVWFFSPRILP
                     SVMRQFDYDTCVDIGICAEGLSFADGVMNKEYCLKEGKKWDEKRKSCNVRESIK"
     gene            2489..2812
                     /locus_tag="PKI94_04635"
     CDS             2489..2812
                     /locus_tag="PKI94_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26059.1"
                     /translation="MKNFSTNFKKIVIGFILLIIILFLLKFIHSVNDFINEDTCVDTG
                     LCAEGLKFGNNILTKEYCLKKHFKWDDKRKECDMAIESRTCREQGYEWITEKGKCSHE
                     IAKDW"
     gene            complement(2822..3757)
                     /locus_tag="PKI94_04640"
     CDS             complement(2822..3757)
                     /locus_tag="PKI94_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26060.1"
                     /translation="MKKLLVIILYFALCGGVFAQGQFCAIISKMETSLFGIQYESQTD
                     EVRLSRIEENVYGKSSSQPLNKRIERLKKDLSADLIGQEIKPKRDTFAEDEDGRKVEE
                     IPKADPNVNYPIVNNLEKEVFKTEYKTLDINKRLSNLEQKAFNKSFSDESLNSRVDRL
                     KAKILPETKLAEKSNESDYFYNPEDLDNLPMQGTMSGAAAPDNFSQKDFGDNFDDYES
                     NADIRAPLTSLEKSLFKKAFPDDTVENRLTRLEAKIFSTSFVQDDPQTRMARIASAQQ
                     AKKSAKKYDGNKFAQHMSTAMQIGAIVLMILACVL"
     gene            complement(3879..4475)
                     /locus_tag="PKI94_04645"
     CDS             complement(3879..4475)
                     /locus_tag="PKI94_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNW26061.1"
                     /translation="MKQDKMSKKIKNGNLINPTWDQRGYIQIYTGNGKGKTTASLGLA
                     MRALGRKWKVLLIMFTKGGDNYGELVSFRNLSKDISNNLKIVQAGLDRIVYSHNKTSE
                     DENAIKKGWELAKKAIKGNKYQLIILDELNIAIDLGIIDIDEVVEVLKNKPKAMEIVL
                     TGRNAHEKAIEVAHLVSKIEPVKHYWDTGIVARKGIEF"
     gene            4502..5035
                     /locus_tag="PKI94_04650"
     CDS             4502..5035
                     /locus_tag="PKI94_04650"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNW26062.1"
                     /translation="MIEKEVLRIKCKEIRRSLDVNSISEKIVETIFSLEEYQKALNIM
                     IFYPLEHEINLMGLMNDSSKNFYLPKVKGEKLLVCPYISGDKLVESKFKTKEPETNPV
                     SSDLLDIVFVPAVAAAKNFNRLGYGGGFYDRFLSKLNPEIIKIIAIPDDLIVDNIPCE
                     SFDETVNIIVSEKQILK"
     gene            complement(5091..5606)
                     /locus_tag="PKI94_04655"
     CDS             complement(5091..5606)
                     /locus_tag="PKI94_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26063.1"
                     /translation="MGFLIAAYRKQDIIRRKYDLEFKEVQLQQLLNDLLSYSSSIADG
                     SVSLSDLSNAPVSVFNRMSNYMTTSHNGSLTTAQQQLSMYKASGQLDSVDAQYRQQYE
                     QMLLKQLYDRERERYQKIEEKILNEKEKKLTAEKTKIDEQLKLLNAELDEVNKLEDAS
                     AKQSAPKFGLG"
     gene            complement(5722..6327)
                     /locus_tag="PKI94_04660"
     CDS             complement(5722..6327)
                     /locus_tag="PKI94_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26064.1"
                     /translation="MKINLISNFKDSYKGNYPLTFKNNFPQYRPLKDELSIRQRFEDD
                     DLDISDEIELLSSKYLTKDENKNIFAHKATSNEIKKMNKALFLYPELIDEIYTTENKF
                     GSLPSYTMDSKKIKEINKVLNPKTFAEVYSHKNNFGDTIMSYFAMNSKMKEEINGLMR
                     KYTKDSKYLTPSTSLKLLENNRIYLDDENMYRLFILSGRNS"
     gene            6441..8141
                     /locus_tag="PKI94_04665"
     CDS             6441..8141
                     /locus_tag="PKI94_04665"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF005482.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNW26065.1"
                     /translation="MLHDRENNMRELSPLQIERLKYQPKLPKALMRGIKNLILIKGHE
                     TEAIRDKEQIKELFKNTYGSPLVTFEGTNEENDSSVKNVGVILSGGQAPGGHNVIAGL
                     YDALKQANYQNKLYGFLGGPAGIIDGKYIEFSDEFIDKYRNTGGFDIIGSGRTKLETQ
                     EQFEKSLEVCKRLNISAVVIIGGDDSNTNAALLAEWFVQNDCGIQVIGCPKTIDGDLK
                     NEQIEISFGFDTATKTYGELIGNIQRDANSAKKYWHFIKIMGRSASHVALEAALQTQP
                     NITLISEEVEEKQMSLNEIINNMAEIIAKRAANGKNFGIAVIPEGLIEFIPEMKSMIA
                     NLNDIMGELEQDFNYNNATSTHERFEIVENRLQENNSKVFHSLPALIKAQLLMDRDPH
                     GNVQVSRIETEKLLIEMIKTKLEEMKSQGQYVGKFSEQAHFFGYEGRCAFPSNFDADY
                     CYSLGFNAFALVNFGLTGYLSSIKNLTESADNWIAGGVPLTVMMNIEKRHGEFKPVIQ
                     KALVKLDGPVFKMLEKNRENWAYNDKYIFPGAIQYFGPSSVCDLTTITLQLEKQKTFV
                     "
     gene            8299..10134
                     /locus_tag="PKI94_04670"
     CDS             8299..10134
                     /locus_tag="PKI94_04670"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF005482.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNW26066.1"
                     /translation="MKIFLNTYKVSSKDATLNKRNGVVRTNNQNYSTDIFQKRINFQG
                     KDFYQNTTLEVSPLQQERLKYIPKLPAVFKEGISCLETINACETDSVDDGDEIKKIFP
                     KTYGQKLLIFKKGKQQPSPQRNVGIILSGGQAPGGHNVITGLYDALKKANPQNKLYGF
                     LNGPAGIIDGKYIEFDDKLINSIRNTGGFDVIGSGRTKLETKEQFERTLENCKKLDIS
                     AITIVGGDDSNTNAAVLAEWFEQKNVPIQVIGCPKTIDGDLKNDQIETSFGFDTATKT
                     YSEIVGNIEKDAASAKKYWHFVKVMGRSASHVALEVALQTHPNVTILSEEVEQKQIPL
                     EEIVDNIAQVIAQRAQNKKNYGVILIPEGLIEFIPEMKTMISDLSDVLSELEENENYE
                     NSSSDLEKYSIISERLQEKNQASAKVFEALPEDIKSQLLMDRDPHGNVQVAKIETEKL
                     LISMIETRLEEMKDRGQYSGKFSAQPHYVGYEGRCAFPSNFDANYCYSLGYDAAALIN
                     SNATGYMACVGGLSSSPDKWKAAGIPLTMMMNMEKRHGQMKPVIKKALVELDGPVFKT
                     FQAQRDAWALNDQYIYPGAIQYFGPSSVSDTITKTLEMERQSGLD"
     gene            complement(10225..12150)
                     /locus_tag="PKI94_04675"
     CDS             complement(10225..12150)
                     /locus_tag="PKI94_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26067.1"
                     /translation="MVEFNPENKLIIQPNANITSGGGTPVPNSNNQSVSPNSIIINNE
                     VTTNTQNLNSIYTTNPQTTTQTQPEQPNEQPFIKTGESLSERLDRYYSGKYSKSNLEQ
                     KRELLRRYMTGHFQALTNKTPAERIRIQLADYKKLLSNTEEPQDYRVLANQIEILESQ
                     NQIPGARSAIEQARNPEFRRNAEIGISEVISDCDSENRSELTRIIVNSDNFEAYESGL
                     NNLEQVHGEQQIQLYEIYSIKVSRDFNTYTQQNTAENQVTQGPLTQDVANSFKPWQWA
                     NMTVEQRNNIQDVSMSNDPNARLNALENGVNSQDPGYAQTFGGFGYKIQPEYQQEASV
                     IIVNTHNIDVINAAADTLPLYTPENQALVHKNYFTNGDITTEHILTSHIGECDINAQL
                     PIYELTMTSTHQETLNYAASLIYTLHPDNQYNATQYTINTGNEEAIGYAAQYANLCNE
                     EVRDEIKNLLYSTGYQSVRYILAYSDTTEEEGKTTTNTSSSSSTNNDEFKKAKLSEKI
                     KIIEKLSKSNNETSIKDLLKNATNAEKIAIISQLPKNILASVVNVIMDSNPSADVMYK
                     ITSMMGNMNDKEQTKLVGNLISRSSSSLLANNVKFLNVISQKIFIEKMAAKGNLSAIN
                     KDDLHLSAKKHYTDLTT"
     gene            12323..12517
                     /locus_tag="PKI94_04680"
     CDS             12323..12517
                     /locus_tag="PKI94_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26068.1"
                     /translation="MDENKILRLRVLERTEETIDEVVEKQLSSGFAYPNQSNPIYKNL
                     AKFHNEPIGKKFSVKDLFGI"
     gene            12533..13021
                     /locus_tag="PKI94_04685"
     CDS             12533..13021
                     /locus_tag="PKI94_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26069.1"
                     /translation="MYEVKKRIFLDLTKSQKSALCNHLRALVKAVNVEADNLLQKFLD
                     DEKYYLEINSSRFEFLKDLIEDEKFLKEALLYLKECKKHYDYKKAQEPLRQAQKEFDK
                     KKRKFLQEIKMSKEKPTKKQLSYYKSLCKKYNIDKQETEELSKLDLKKMIEEIVNEYK
                     RD"
     gene            13002..13874
                     /gene="prmC"
                     /locus_tag="PKI94_04690"
     CDS             13002..13874
                     /gene="prmC"
                     /locus_tag="PKI94_04690"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNW26070.1"
                     /translation="MNTKEIEKILIQGGIEPNEAKIEAKILIKHFFGVNDVDLVLEKE
                     FDESRREDLLLAANLRAQKRIPIQHILGTAYFMGEDFIVNEHVLIPRDETELLVKQAV
                     EIIKSKQICRCEEYSDEAIQNNIKVLDIGTGSGCIACMIAKLSGAQVIGVDISREALG
                     VALDNSSKLGLFNKAIFRKSDIFSNVKVDEKFDLIVSNPPYIPKSEKEKLQKEVGFEP
                     DIALFADNNGLDFYQKISKQSPKFLKNGGYLIFELGVGQSQEVFKLMSEAGFKEIEIQ
                     KDLAQIDRVIWGRI"
     gene            13940..14110
                     /locus_tag="PKI94_04695"
     CDS             13940..14110
                     /locus_tag="PKI94_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26071.1"
                     /translation="MARLIRPTWAIRCVQSGCRELFEVAVIEDGRQQEVICPACGEHY
                     VYPIRKEDENAQ"
     gene            14121..15071
                     /locus_tag="PKI94_04700"
     CDS             14121..15071
                     /locus_tag="PKI94_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNW26072.1"
                     /translation="MFFNETNKRYNQYSEHLKQKFGVKVYKITIDAGFSCPNRDGRIS
                     DGGCIFCDEGGSFSQAHSNQLSIEQQVETGVATLSKRFKAKKFMSYFQAYSNTYKPVA
                     ELEKIYKSALIHPDIVGLSIGTRPDCVDGEKLKLISSFKDDYYTWVEYGLQSIHDKTL
                     KAINRGHDFECFLKAYEAAKNFGINVCAHVILGLPGETKEDMLETAKKLAQLKVDGVK
                     IHMLCALKGTKLSQMYEEGKISFMSEDDYINTVCDFLEILPPETTIHRLAGNGLKKNL
                     IAPKWIGKKLDALNKIDRELEKRNSCQGGKCTPAFCINQK"
     gene            15112..15567
                     /gene="smpB"
                     /locus_tag="PKI94_04705"
     CDS             15112..15567
                     /gene="smpB"
                     /locus_tag="PKI94_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNW26073.1"
                     /translation="MNKKVITTNRKAYHDYMIFDKLIAGIVLTGTEIKSIRKGAINLK
                     DSFAKIENGEIFLYNCHISPYEQGNRYNHEPERVRKLLLNKKEILKMLGKIKKEGYTI
                     VALELFLTNGFAKLEIGLAKGKKLHDKREDIAKKSQNRDMQRAMRGKLA"
     gene            15688..16296
                     /locus_tag="PKI94_04710"
     CDS             15688..16296
                     /locus_tag="PKI94_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26074.1"
                     /translation="MMYSKKDILRSLKELNYYIDEQILNNFIKNWKIDAIYEDEDGVE
                     FFDNLSIVKIKKGISLKSQGYDNDEIIYHIGKILAEKHFDEIKEKPVKEKLILSNEIL
                     PASISATEVKTTSLDTTNENLKILADVIAQKVTEELSERLDKIEFSQNMKKESKLEQD
                     NEKLSQKVEELLEDNKQLAQRVEQLERRKNYFVEFFKKYFVF"
     gene            complement(16293..17162)
                     /gene="miaA"
                     /locus_tag="PKI94_04715"
     CDS             complement(16293..17162)
                     /gene="miaA"
                     /locus_tag="PKI94_04715"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNW26075.1"
                     /translation="MKEKSLNKKKIIAIVGPTASGKSRLAIRIAKKNNGEIISADSRQ
                     IYKGFDIATAKPSIEEMEGIEHYLIDIVEPEIDYSVANFYNDAKAAIEKIYSKGKIPI
                     VVGGTGLYFRILLENFSPPKVAPDYELRAELEKLESKKLHKMLEKLDLQSAQKIHFNN
                     KVKIIRAIEVSLTLQSPHSKAAGKKEPEFEVEWIGLNPSNRADLYKRIDLRVDEMIKK
                     GLVEETKALLKKHGRINNFINTIGYQEILEYLDGKISLNEAILLIKQNSRHYAKRQLT
                     WFRKNKLINWQEA"
     gene            complement(17062..17964)
                     /locus_tag="PKI94_04720"
     CDS             complement(17062..17964)
                     /locus_tag="PKI94_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26076.1"
                     /translation="MIKYFSKAFKITNENIILATPLVLFLFILSLYIEFTKRAPENVA
                     AAILLLVTVLFMIGAFFAGWLFMTKQAVKISNQVFEDEEEKAKASFNLLKDFNYGIGE
                     FFLPFVGAAILYGTILVVIVIAAYKAGMHFIGDPGIDFVQIKRALTSTAEMKALITSL
                     STQQLAKINAWNFMLIGVTFVQSFLTMFWGCEIIYRTKNPIIALFKSIQSIFKKPLGA
                     IILFLYISFVNFIVSFINAILAFNSILYFLSMLLYFYFIVYIIVLIFFYYEREILKQE
                     ENNSNSRTDSIGQKPPGDSDSKEE"
     gene            complement(17966..19105)
                     /locus_tag="PKI94_04725"
     CDS             complement(17966..19105)
                     /locus_tag="PKI94_04725"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNW26077.1"
                     /translation="MKKSKTEKQKIKVAFPHMGTIYIAWSAGLKKMGVEPFVSPYTNR
                     KTLSLGTKYSPEAICLPYKLILGNFIEAIENGTDYVAMITSPGICRLGEYGSSIRNNL
                     KDLGYDAKYIELALYDGIKGMYNFAKELTGKNDPILFIRAILATIKTIFVLDDLETAL
                     SYYRARENHFGDAEKNFKKGLKYVLEASNSKELKQAKKMALEEIAKTKIDKNREILHV
                     DITGEIFLVLDYFSNQHIERELGKMGVQTRRSLTVGSFLKDAIIPKIFRKGETHLQRA
                     FRMAKPYLMRDIGGDALECVSDVAWASEKGKDGLIHISPFTCMPEIMSQNIFPNMRED
                     CNIPVLSLIMDEQTGKAGYITRLEAFVDLMRRRKRQEGNKQKAKV"
     gene            19646..19954
                     /locus_tag="PKI94_04730"
     CDS             19646..19954
                     /locus_tag="PKI94_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26078.1"
                     /translation="MTIQTTESMPIQSSNYRGVPPIPQGRTNENYIHSIHTTNNTTTI
                     INNYYTVPTKSNTSMHKKNNDVLTINISKSRAKDTIFFMVAPFHYITIKGVKCLKKLF
                     "
     gene            20005..20211
                     /locus_tag="PKI94_04735"
     CDS             20005..20211
                     /locus_tag="PKI94_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26079.1"
                     /translation="MTIQKTTFNATQQARIIKKEKLNSLEKMGLIFAGGILGPTLLGV
                     NEVMHPGSIKKVAKKAYEGIKNIF"
     gene            complement(20265..>20569)
                     /locus_tag="PKI94_04740"
     CDS             complement(20265..>20569)
                     /locus_tag="PKI94_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNW26080.1"
                     /translation="FKEKNLSGIEFYDNILIIEDIVDSGRTMNLLLSKLSKEYPDKDI
                     KICTLLDKPDARFPENKDLKLDYVGFKIKKDFVLGYGLDYYEDGRELENIYKVKFK"
     assembly_gap    20570..20669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20691..20942)
                     /locus_tag="PKI94_04745"
     CDS             complement(20691..20942)
                     /locus_tag="PKI94_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26081.1"
                     /translation="MKKKITTKDLIKEFFTPEEAQEIQKEIVQEVEKIKWGGKRPHAG
                     RKPKTGVVLEFRIRVSEKEKQFIDYARSHNLNFDKLMKG"
     gene            complement(21325..22746)
                     /gene="gcvPB"
                     /locus_tag="PKI94_04750"
     CDS             complement(21325..22746)
                     /gene="gcvPB"
                     /locus_tag="PKI94_04750"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNW26082.1"
                     /translation="MTKTIFEKSNNLGINIKDIEEKADFLNKKFLRNTPLNLPQVSEL
                     EALRHYKELSDKNFCIEKGFYPLGSCTMKYNPKVNEFLANLEGFTNLHPLQSDEDSQG
                     ALELMYQLQCALKTITGMDSISLQPAAGAHGELAGMMIIKKYFESRNEKRTKVIIPDS
                     AHGTNPASAKMCGFECVEIKSNKKGQVDIDALKAVLDENVAAIMMTNPNTLGLFEENI
                     LEISDLMHKNGSLLYYDGANFNAIMGYTNPKLMGFDVVHINLHKTFATPHGGGGPGSG
                     PIGVVGKLKDFLPIPIIEFDGDKYIRNYNLPNSIGKVKSFYGNFGVLVRAYAYILMTG
                     KNLKIVSEDAVLNANYLKEKLKDTYELPYDYTCMHEFVLSGEKQKEQGVSTLWIAKRL
                     MDENTHPPTVYFPLIVHEAIMIEPTETENKQRLDEFVEVMLKIAKEIKQSSQEVLKSP
                     KTTPVKKIDETLAARQPDLRWNK"
     gene            complement(22758..23369)
                     /locus_tag="PKI94_04755"
     CDS             complement(22758..23369)
                     /locus_tag="PKI94_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW26083.1"
                     /translation="MHTEKAVKTRLKDYKTLIEVIAKVEYQKLNCAHLIDYSEIVNIG
                     THTLYLLFKNNNPEQFNTSYLSTAIKWAIRNEVRRRYKWYSLKNKKECDETEQEDLRE
                     ALYKTILSIDEMADAENPKHIKDESKNPEESLEFFELKAEIIAAIRKLPQRERDLIES
                     KFFKDKKLKDIAHDFDISPSRISRIIQVGLNKVKKDLIKNNVV"
     gene            23485..24342
                     /locus_tag="PKI94_04760"
     CDS             23485..24342
                     /locus_tag="PKI94_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNW26084.1"
                     /translation="MKALFCTEGSDISLLALENVSKYLKNIVLDIICVIDWSFLPDSM
                     NIDRETYSRTYESIADSVLNFAEDAIKEKGLSVGKKVKNFGSPVEGILEQVQNDSYDL
                     IIMGSHGKKGLQKWLGSVSRQVLTNVSMPVFISKRKNEGKKILIAIDGSEISYSAVKQ
                     ATELFDFTNKEIHVVSVKETPELLPVEVALDKKWIDDIDKQQKIHASKAINKVKSILA
                     SSNLSVINEVILTGHPAHKVIEFAQQENIDLIVMGARTKASLSTFLLGSVSKRVLENT
                     NSDVLIVRK"
BASE COUNT         8078 a         3866 c         4493 g         8489 t
ORIGIN      
        1 tatgtttttg atgccgagca aatttgaacc ttgcggactt agccaaatga tagctatgcg
       61 ctacggcact ttgccgattg taagaacgac aggcggatta gaagacagcg ttgtggggca
      121 tcctttaaaa cacgcgaacg gatttaagtt ctgggattat gattcaacca atatgttgaa
      181 cggaattaag tatgcaataa atgtcttcaa ggacaaacaa gagtgggata agcttgttaa
      241 atcggcgatg aactatgatt acagctggaa gaaaagcgca aagcactaca tggacgttta
      301 cgaagggctt ttgaagtcat aaattctagt atataaaatt gtcgaacgat ttgattgttt
      361 tacttaacga taagtgattt aaaattacgt atttttacgg actttaaatg ccttttaaaa
      421 gttgttatta ttatataatg acaacttagg agaatttatg tcaaaaaact tacaaaatca
      481 aataggtaaa attaaaaaat caattgttaa taataatata acaaataatt caatggcaac
      541 ttatggaaag ttgaaagaaa ttgaacattc attatataga catggtatgc tttcaaaagc
      601 aggcttgaag gatgaggcta aggctgaaag tgcgaaagca aacagtttaa ttagtaattt
      661 taacagcagc gaagacacag aaacattaaa aggctatata agtaatacaa aatacgtttg
      721 ggtagcaagc gaagacagtt gtgaagcttg ccaagaattg gatggcaccg aatatgattc
      781 aatggacgat attccagaca aacctcatcc taattgcaac tgttatattg aagaaattga
      841 aatggaagac gatgaaaacg gcaatggggg cggagatagc aatgacaatg gcaatggtgg
      901 tggcggtgga aataaccgag gcgaaccttg tgattgttgg gataaaatag atgaaatggt
      961 cgaagaggca aatgctcttg atgatgaatt agcttctgat atgagtgata ttgattcaat
     1021 tcaggcagaa gccgaaggtt ggcttgtcga ggttcaagaa ttaaaagcac aagtagaaca
     1081 cgctcaggaa gcattagaag ctatagaacc atgtggccct gattgtgcaa ttacagggat
     1141 ggctgttaat ataacagatg ataaagagct tgaatatact gtgtataatt taattaaaga
     1201 tattgaagaa gcaagagttg tttatgaagt tttccaatca aataagcatg aaatggaaac
     1261 aacagccaat tcttttgata aatattatca tgcgaaagca aattgtgaat ctgctgaact
     1321 aggaacaatt gaaactatgt gggcagttgt gtttagtatt tttaaagaag taaaagattt
     1381 taaaacaaaa gtattttccc tccatatgga tgctcaaaaa gtctttcttg attgtatgaa
     1441 tgatttgaga gccgatttat acgggttaat gaaagcaaaa gaacatggtt attgttctga
     1501 taaattaaaa gatattgata aagtttttaa aaaataaaaa atattttaat atataatgat
     1561 aaaatgaaag ataaaattaa agttgtttta aacaaaatat tacaagtatg ctcaataatt
     1621 tttaaaactt tagcatgtat agttttaact tattttattt taattctacc aataactgct
     1681 attagaacag gaaaatttga ttggcagatt tttattatca ttgtagtttg ttttttattt
     1741 gctttcttaa ccaattgggc aatattgaaa aaaggctgtt taagaaaaat attgatgttt
     1801 tttgttttat ttttaatttg tgcagtattg gaaattaaaa tgcttcatta tcttggcatt
     1861 gaaagtgatt attgcattga agatggagac tgtaaagaag gtcggatagt caatacagaa
     1921 aaatacggaa aagttctaat aaacaaggaa aattgcctaa aatacaattg ggaatggtac
     1981 gaaaaacaaa aatattgtaa gctatggaat tgaatatgca aaaaatgaaa gtttttttga
     2041 caaaattatt tttaatatta ttccgaatta tagatacagt tgtaaaaata tttatttgtt
     2101 taggtattac gatttttgta gggtatttcg ctgcgatagc aagtgcttcc gccttcgcgg
     2161 gtaggcaggg atttgattgg caagttttat tgtatgttct tgcttctgta tttacgattt
     2221 tggggatttg gttagtagca tttattaaaa caaaaaaaaa tacaaaaatt atttatgcag
     2281 taatattttt cgtttggttt ttttctccca gaatattgcc ttctgttatg cgacaattcg
     2341 attatgatac ttgcgttgat attggaattt gtgcagaagg attaagtttc gccgacggtg
     2401 taatgaataa agaatattgt ctcaaagaag gtaaaaaatg ggatgaaaaa agaaaaagtt
     2461 gtaatgtaag agaaagcata aaataagtat gaaaaatttt tcaacaaatt ttaagaaaat
     2521 agtcattggc tttattttat tgataatcat acttttttta ttaaaattta tacattccgt
     2581 aaatgatttc ataaatgagg atacttgtgt agatacaggt ttatgtgccg aaggtttaaa
     2641 atttggaaac aatatattga ctaaagaata ttgtctaaaa aaacatttta aatgggatga
     2701 taaaagaaaa gaatgcgata tggctattga atctagaact tgcagagaac aaggctacga
     2761 atggattact gaaaaaggca aatgcagcca tgaaattgcc aaagattggt aataacttat
     2821 tttataaaac gcaggcgaga atcatcaaaa ctattgcccc aatttgcatt gcagttgaca
     2881 tgtgttgcgc aaatttattg ccgtcatatt ttttggcact cttttttgcc tgttgagcac
     2941 ttgcaattcg agccattctg gtttgcgggt catcttgcac aaagcttgtg ctgaaaattt
     3001 ttgcctcaag tcttgtaagt ctgttttcaa ctgtatcgtc aggaaaagct tttttaaata
     3061 aggatttttc aagtgatgta agcggcgctc ttatatcagc attgctttcg taatcatcaa
     3121 aattgtcgcc gaaatctttt tgagaaaaat tatcaggtgc ggcagctccg ctcattgttc
     3181 cctgcatagg taaattatca agatcttcgg gattgtaaaa ataatcactt tcatttgatt
     3241 tttcagctaa ttttgtctca ggtaatattt tggcttttag cctgtcaact ctggagttta
     3301 agctttcatc agaaaaactc ttgttgaaag ctttttgctc taaatttgaa agtcgtttgt
     3361 ttatatcaag ggttttgtat tccgttttaa aaacttcttt ttctaaattg tttacaatag
     3421 gataatttac gtttgggtca gccttaggta tttcttcgac ctttcgaccg tcttcatctt
     3481 ctgcaaaagt gtcccgtttt ggttttattt cctggccgat taaatcagcc gataagtctt
     3541 tttttaaccg ttcaatcctt ttgttcagcg gttgagaaga agattttccg taaacatttt
     3601 cttcaatgcg gctaaggcgt acttcgtcag tttggctttc gtattgtatg ccaaaaagcg
     3661 atgtttccat ttttgaaata attgcacaaa attgaccctg tgcaaaaact cccccgcaaa
     3721 gtgcaaaata taaaattata accagtaatt ttttcataaa tttatcctga tttcaggata
     3781 aaaattattg aagtaaattc ccattcctca acgtctttat tcaaaaaaag aaaaaatcag
     3841 cataattatt taagcagaaa gtagtataaa atattaccct aaaattcaat tccttttctt
     3901 gccacaattc cggtgtccca atagtgcttc acaggctcga tttttgaaac caaatgtgca
     3961 acttcaattg ctttttcatg ggcgtttctt ccggtcaaaa caatctccat agctttgggt
     4021 ttgtttttta aaacttccac aacttcatca atgtctatta tgcccaaatc aatagctata
     4081 tttagttcgt caagaattat aagctgatat ttatttccct ttatggcttt ttttgcaagt
     4141 tcccaaccct ttttaattgc gttttcgtct tcagaggttt tgttatgcga ataaacaatt
     4201 ctgtctaagc ctgcttgcac tatttttaaa ttattggaaa tgtcttttga aagatttctg
     4261 aaagaaacca gttccccgta attatctccg ccttttgtaa acataataag aagcactttc
     4321 cattttcttc ccaaggcacg cattgcaagc cctaatgatg ctgtggtctt gcctttaccg
     4381 tttcctgtgt aaatttgaat atatccgcgc tgatcccagg ttgggtttat taaatttccg
     4441 ttcttgattt ttttgctcat cttatcttgt ttcacttccc catttattat atattaaaaa
     4501 aatgatcgaa aaggaagttt tacgcattaa atgtaaagaa ataagacgct cgctggatgt
     4561 taattccata agcgaaaaaa tcgttgaaac gattttttcg cttgaagagt accaaaaagc
     4621 cttaaatata atgatttttt atcctttgga acatgaaatt aatcttatgg ggttaatgaa
     4681 tgattcttca aaaaacttct acctgccgaa agtcaaaggt gaaaaacttt tggtctgtcc
     4741 ttacatctcg ggtgataaac ttgtcgaatc aaaatttaaa accaaagagc ctgaaacaaa
     4801 tcccgtttct tcggatttgc ttgatattgt ttttgttcct gcggttgctg ctgcaaaaaa
     4861 ctttaacagg cttggctacg gcggaggctt ttatgacagg tttttgtcaa aattaaatcc
     4921 tgaaataata aaaataattg caattcctga tgacttgatt gttgacaata ttccttgcga
     4981 atcttttgat gaaacggtta atataatagt ttccgaaaaa cagattttaa agtagaaaaa
     5041 ggctcgcaaa ctttagtttg cgagcctttt tcttattatt taattatttc ctaacccaga
     5101 ccgaattttg gtgcagattg tttagctgat gcatcttcta atttatttac ttcgtcaagt
     5161 tcggcattga gtaattttaa ttgttcatca attttggttt tttctgcggt tagctttttc
     5221 tctttttcat tcaagatttt ttcttcgatt ttttgatacc tttcacgttc tctgtcgtat
     5281 agttgtttta aaagcatttg ttcgtactgt tggcggtatt gagcatcaac actgtctagt
     5341 tggcctgacg ctttatacat tgagagttgt tgttgagcag tagttaaaga accgttgtga
     5401 gaagttgtca tatagtttga cattctgtta aaaaccgaaa caggcgcatt tgataaatca
     5461 cttagcgaaa cacttccgtc agcaatgctt gatgaatagc tgagcaaatc gtttaaaagt
     5521 tgctgtagtt gaacttcctt aaattcaagg tcgtattttc ttcgtatgat gtcctgtttg
     5581 cgatatgcgg caattaagaa acccattatt ctactcctac caaattaaat taaccttaac
     5641 cttacatata taaaaacatt tgtttttgat taattgcctt ttacatattt ttttgtaacg
     5701 aaatgttaac aaaattaaaa attaagagtt tctgccggac agaataaata acctgtacat
     5761 attttcatca tccaggtaaa ttctgttatt ttccaataat tttagcgatg tgctgggtgt
     5821 taaatatttt gaatcttttg tgtatttacg cattagtccg tttatttctt ctttcatttt
     5881 tgaattcatt gcaaaataag acattatggt atcaccgaaa ttatttttat gggaataaac
     5941 ctcagcgaat gttttaggat tgagtacttt gtttatttct tttatttttt ttgaatccat
     6001 agtgtaagaa ggcaaactgc cgaatttatt ttctgtggtg tatatttcat caattaattc
     6061 aggatataaa aacaaagctt tattcatttt tttaatttca tttgaggttg ctttgtgtgc
     6121 aaaaatattt ttattttcgt ctttggttaa atattttgaa cttaataatt ctatttcgtc
     6181 tgatatatcc aaatcatcat cttcaaatct ttggcggata gataattcat cctttaaggg
     6241 gcgatattgg ggaaaattgt tcttaaatgt taaaggataa ttgcctttat aagaatcttt
     6301 aaaatttgaa attaaattga ttttcatagt tacctcactt ttgtttgcat tatattaaaa
     6361 tactaacaaa aaaaaatttg ctaccaaata attaattgat acaaaaatta actcttatat
     6421 ttttgttata aaatattatt atgttacatg atagggagaa taacatgaga gaactttcgc
     6481 cgttacagat tgagagatta aaatatcagc ccaaattacc aaaagcattg atgcggggaa
     6541 taaaaaatct tatacttata aaaggacatg aaacagaagc cataagagac aaagaacaaa
     6601 taaaagaact ttttaagaat acttacggaa gcccgttagt gacttttgag ggtacaaatg
     6661 aagaaaatga ttcttctgta aaaaatgtgg gagtaatcct ttccggcgga caggctccgg
     6721 gcgggcacaa tgttatagca gggctctacg atgcacttaa acaggcaaac tatcaaaaca
     6781 aactttacgg atttttaggc ggaccggcag gaattattga tggaaaatat atagagttta
     6841 gtgatgaatt tattgacaag tatagaaaca caggcggctt tgatataata ggctcaggca
     6901 gaaccaagct tgaaactcag gaacaatttg aaaaatcgct tgaggtttgc aaaagattga
     6961 atatttctgc ggttgtaata atcggcggcg atgattccaa cacaaatgcc gcacttcttg
     7021 ccgaatggtt tgttcaaaac gattgcggga tacaagttat cggctgtccc aaaaccattg
     7081 acggtgattt aaaaaatgag cagattgaaa tttctttcgg ctttgacacc gctacaaaaa
     7141 cgtatggaga attaatcgga aatattcaaa gggatgcaaa ttcggcaaaa aaatattggc
     7201 actttataaa aattatggga agaagtgctt cgcatgtggc acttgaagct gcacttcaaa
     7261 cacagccaaa tatcactttg atttcagaag aagtcgaaga aaaacagatg tccttaaatg
     7321 aaattataaa taatatggct gaaattattg caaaaagagc agctaacgga aaaaatttcg
     7381 gtatagccgt tattcccgaa ggtttgattg aatttattcc cgaaatgaaa tctatgattg
     7441 ccaatttaaa cgatattatg ggcgagttgg aacaggattt taattataat aacgctacat
     7501 ctactcatga aaggtttgag atagttgaaa acaggcttca ggaaaacaat tcaaaggtct
     7561 ttcattctct gcccgcgctt ataaaagctc aacttcttat ggacagggat ccgcacggga
     7621 atgttcaggt ttcaagaatt gaaacagaaa aacttttgat agaaatgatc aaaacaaaac
     7681 ttgaagaaat gaaatcacaa gggcagtatg tcggtaaatt ttctgagcag gcgcacttct
     7741 tcggttatga aggcagatgc gctttccctt caaattttga cgccgattac tgttattcgc
     7801 tcggttttaa tgcttttgca ttagttaatt tcgggcttac aggttatttg tcttcgatta
     7861 aaaatctgac agagtccgct gataattgga ttgcgggggg tgttccttta acggttatga
     7921 tgaatattga aaaacgtcat ggcgagttta agccggttat tcaaaaagct ttggttaaac
     7981 ttgacggacc ggtctttaaa atgcttgaaa aaaataggga aaattgggct tataatgata
     8041 aatatatttt ccccggagct attcaatatt ttgggccaag ttctgtttgt gatttaacaa
     8101 caataactct tcaacttgaa aaacaaaaaa cttttgttta aattttttat aaaatgcaaa
     8161 ggcggcttgt ataacaagcc gctcctgctt tttgtggaat tgctaaaaaa tgtaaatttt
     8221 tatatttttt cttgcctaca aatagcaaaa aaaaatatgt ttgaatttaa aatatttgta
     8281 caatagagaa ggtggaatat gaaaatattt ttgaatacgt ataaagtaag ttcaaaggac
     8341 gcaacattaa ataaacgcaa cggggttgtt agaactaata accaaaatta ttcaacggac
     8401 atttttcaaa agagaattaa ttttcaaggg aaagactttt atcaaaatac aacgcttgaa
     8461 gtttcaccgc ttcaacagga aagattaaaa tatattccaa agcttcctgc tgtatttaaa
     8521 gagggtattt cttgtttgga aacaataaat gcctgtgaaa ctgattctgt tgacgatgga
     8581 gatgaaatta aaaaaatatt cccaaaaact tacggacaga agttgttaat atttaaaaaa
     8641 ggaaaacaac aaccttcgcc acaaagaaac gtaggtataa ttctttcagg cggacaagca
     8701 ccaggcggtc ataatgttat aacagggctt tacgatgctc ttaaaaaagc aaatcctcaa
     8761 aataaattat acgggttttt aaacggtcct gcaggaataa tcgacggtaa atatatagaa
     8821 tttgacgata aattaataaa ttctattcgc aataccggcg gttttgatgt tataggttca
     8881 ggcagaacaa aacttgaaac taaagaacag tttgaaagaa ctttagaaaa ttgtaaaaaa
     8941 cttgatattt ctgcaataac aatagtgggt ggagatgact ccaacacaaa tgcagcagtt
     9001 ttagccgaat ggtttgaaca aaaaaatgtt cctatacagg taatcggttg tcctaaaact
     9061 attgacggtg atttaaaaaa tgaccaaatc gaaacatctt tcggatttga taccgcgaca
     9121 aaaacttatt cggaaattgt cggaaacata gaaaaagatg ctgcgtctgc taaaaaatac
     9181 tggcattttg taaaagttat gggaagaagt gcctctcatg tcgcgcttga agtggcactt
     9241 caaacacatc ctaacgtaac aattttgtct gaagaagttg agcaaaaaca aattcctctc
     9301 gaagaaatag tcgataatat agcacaggtt attgcacaaa gggcgcaaaa taaaaaaaat
     9361 tacggagtaa tattaattcc tgaaggtctt atagagttta ttccggagat gaaaacgatg
     9421 atttccgatt taagtgatgt tttatcagaa cttgaagaaa atgaaaacta tgaaaattct
     9481 tcttccgatt tagaaaaata ttcaattatt tcagaacgtt tgcaagaaaa aaatcaggca
     9541 agcgcaaagg tttttgaagc tcttcccgag gatataaaaa gccagcttct tatggataga
     9601 gaccctcatg gcaatgttca ggttgcaaaa attgaaactg aaaaattgct tatatccatg
     9661 atagaaacaa ggcttgaaga gatgaaagac agagggcaat attcaggtaa attttccgca
     9721 cagcctcatt acgtgggtta cgaaggaaga tgcgcatttc cttcaaattt cgacgcaaat
     9781 tattgttatt ctttaggtta tgatgctgcc gcattaataa attcaaatgc aaccggatac
     9841 atggcttgtg tcggtgggtt gtcttcttct cctgataaat ggaaggcagc agggattcct
     9901 ttgacaatga tgatgaatat ggaaaaacga cacggacaaa tgaaaccggt aatcaaaaaa
     9961 gccttagttg aacttgacgg acctgttttt aaaacatttc aagcacaacg agatgcttgg
    10021 gcactaaacg atcaatatat ttatccgggt gcgattcaat atttcgggcc gtcatctgta
    10081 agtgatacca taacaaaaac gcttgaaatg gaaagacaaa gcggattaga ttaaaattac
    10141 cgtgagcaag tgtaaaaaag aggcgaaaat cttttgattt tcgccttaaa ttcaacctac
    10201 cgtttccctt ttccctattc gtaattatgt tgttaaatcg gtataatgct ttttcgcgga
    10261 caggtgcaaa tcgtctttgt ttattgcaga aagatttcct tttgccgcca ttttttctat
    10321 gaatattttt tgtgatatta cgtttaaaaa ttttacgtta tttgcaagca agcttgaaga
    10381 acttcttgaa ataaggttac cgaccagttt tgtttgttct ttatcattca tgttgcccat
    10441 catacttgta attttataca taacatcggc agaagggttg ctgtccataa ttacgtttac
    10501 aacagaggcc agtatgtttt taggtagttg cgaaattata gcaatttttt cggcatttgt
    10561 tgcgtttttg agtaaatctt ttattgatgt ttcattattt gacttgctta gcttttctat
    10621 aattttgatt ttttccgaaa gttttgcttt tttaaattca tcattatttg ttgatgatga
    10681 agaacttgta tttgttgtag tttttccttc ttcttctgtt gtatcggaat atgctaaaat
    10741 gtatcttacg ctttgataac cggtgctgta aagtaaattt ttgatttcat cacgtacttc
    10801 ttcgttgcaa agatttgcat attgggcagc gtatcctatt gcttcttcgt ttcctgtatt
    10861 tattgtgtat tgtgtggcat tgtattggtt atcgggatgc aatgtataaa ttaatgatgc
    10921 agcgtaatta agggtttctt gatgtgttga tgtcattgtc aattcataaa tcggtagttg
    10981 cgcatttatg tcacattctc caatgtgact tgttaaaata tgttcggtgg taatatctcc
    11041 gtttgtaaaa taatttttat gaactaatgc ttgattttca ggtgtatata atggtaatgt
    11101 gtctgcggca gcatttataa cgtcaatatt atgagtatta actattatga cacttgcttc
    11161 ttgttgatat tcgggttgga ttttatatcc aaatccgccg aaagtttgtg cataaccggg
    11221 gtcttgagaa ttaactccat tttccaaggc atttaatctt gcatttgggt cattggacat
    11281 tgaaacatct tgtatgttat ttctttgctc aacagtcata tttgcccatt gccaaggttt
    11341 aaagctgttt gcaacatctt gtgtaagagg tccttgtgta acttgatttt cggctgtatt
    11401 ttgttgtgta taagtattaa aatctcgaga aacttttata gaataaattt catataattg
    11461 aatttgttgt tcaccatgga cctgttcaag attattcagt ccggattcat atgcttcaaa
    11521 gttgtcggaa ttaactatta tacgagttaa ttcgcttcta ttttcagagt cacaatctga
    11581 tattacctca gaaattccta tttccgcatt tcttcgaaac tcaggatttc ttgcctgctc
    11641 tatggcagat cttgcacccg gaatttggtt ttgggattcc aaaatttcga tttggtttgc
    11701 taaaactctg taatcttggg gttcttctgt attagataat agttttttat aatcagctaa
    11761 ttgtattctg attctttctg caggtgtttt atttgttaat gcttgaaaat gccctgtcat
    11821 atatctgcgc agcaattcgc gtttttgttc aagattgctt tttgaatatt ttccgctgta
    11881 gtatctgtct aatctttcac ttaaagattc acccgtttta atgaaaggtt gctcatttgg
    11941 ctgttcgggt tgtgtttgag ttgttgtctg cgggtttgtt gtgtatattg aatttaaatt
    12001 ttgtgtgttt gttgttactt cattatttat gattattgaa tttgggctca cagattgatt
    12061 gttagaatta ggcacaggag tgccgccgcc gcttgtaatg ttggcgttgg gttgaattat
    12121 gagtttgttt tcagggttaa attcgaccat attccctcgt gtttttatag attatatata
    12181 taataagtat ataatttgtt tttaattgct ttttttttgt tactttgggc atgtttttac
    12241 aaaagtttac atttgatttt tagcaatatt ttcagtgaaa atttttttat atttatataa
    12301 gtaaatatgg tataattttt ttatggacga aaataaaatt ttaagattaa gagttttgga
    12361 aagaaccgaa gaaacaattg atgaagttgt tgaaaaacag cttagttcgg gctttgctta
    12421 tcctaatcaa tctaatccga tttacaaaaa tctggcaaaa tttcacaatg agccaatcgg
    12481 taaaaagttt tcggtaaaag atttatttgg tatttaattt caggaaaaat cagtgtacga
    12541 ggttaaaaaa agaatttttt tggatttgac aaaatcccaa aaatccgctt tatgtaatca
    12601 tttgagagcg ttggttaaag ctgtaaatgt ggaggcagac aatcttttgc aaaaattttt
    12661 ggatgatgaa aaatattatc ttgaaattaa ttcaagcagg tttgaatttt taaaagatct
    12721 tattgaggac gaaaaatttt taaaagaggc tttgctttat ttaaaagagt gcaaaaaaca
    12781 ctatgattat aaaaaagccc aagaaccctt gaggcaagca cagaaagaat ttgataagaa
    12841 aaaacggaag tttttacaag aaattaaaat gtcaaaagaa aagccgacca aaaaacagct
    12901 ttcttattat aaaagtttgt gcaaaaaata taatatagat aaacaagaaa cggaagagct
    12961 ttcaaaactc gatttaaaaa aaatgattga agagattgtg aatgaataca aaagagattg
    13021 aaaaaatatt aatacaaggc ggtattgagc ctaatgaagc aaaaattgaa gcaaaaattt
    13081 tgataaagca tttttttggg gtaaatgatg ttgaccttgt gttggaaaaa gaatttgatg
    13141 agtcaaggcg cgaagatttg ctgcttgcag caaatcttcg cgcccaaaaa agaattccta
    13201 tccagcatat tttgggcact gcttatttta tgggcgagga ttttattgta aacgaacatg
    13261 ttttaattcc acgagatgaa acggaacttt tggtaaaaca agccgttgaa ataataaaat
    13321 caaagcaaat ttgtcgttgc gaggagtata gcgacgaagc aatccaaaat aatataaaag
    13381 ttcttgatat cggcaccggt tcgggatgca ttgcctgtat gatagcaaaa ttaagcgggg
    13441 cgcaagttat tggggttgat atatcaagag aggctcttgg ggttgcactt gacaattcat
    13501 caaaattagg gctttttaac aaggcgattt ttaggaaatc tgatattttt tcaaatgtta
    13561 aagtagatga aaaatttgac ttaattgtgt caaatccgcc ttacattcca aaaagtgaga
    13621 aagaaaaact tcaaaaagaa gtagggtttg agcctgatat tgctttgttt gcagacaata
    13681 acggactgga tttttatcaa aaaatttcaa aacaatctcc caaattttta aaaaatggcg
    13741 gatatttgat ttttgaactg ggtgtgggac aaagtcaaga agttttcaaa ttaatgtcag
    13801 aggcggggtt taaggagatt gaaattcaaa aggatttggc acaaatagat agagttattt
    13861 ggggaagaat ttaataattg ccttaagact taaaacatgg taaaatcagt ttgtaaaatc
    13921 agaattgaag ggaaaaaata tggcaagatt aataagacca acttgggcaa tcagatgtgt
    13981 gcaatcagga tgcagagaac tttttgaagt ggctgttata gaagacggaa gacaacagga
    14041 agttatttgc cccgcatgcg gcgaacacta cgtttatcct atcagaaaag aagatgaaaa
    14101 cgctcaatag cgataatcaa atgtttttta acgaaacaaa taaacgctat aatcaatata
    14161 gcgaacattt aaaacaaaaa tttggcgtca aggtttataa gataaccatt gacgccggtt
    14221 tttcatgccc taaccgtgat ggtaggatat ctgacggcgg ctgtattttc tgcgacgagg
    14281 gcggaagttt ttcgcaagct cattccaatc aactttcgat tgaacaacag gttgaaacag
    14341 gggttgcaac tttgtcaaaa agatttaagg caaaaaaatt tatgtcttat tttcaggcgt
    14401 attctaatac gtataaaccc gttgcagaac ttgaaaaaat ttataaatca gcacttattc
    14461 atcctgatat tgtggggctt tcgataggca ctcgccctga ttgtgttgac ggggaaaaac
    14521 ttaaacttat ttcttcgttc aaggatgatt attatacgtg ggttgaatac gggcttcaat
    14581 caattcatga caaaacttta aaggcaatta accgagggca tgattttgaa tgttttttaa
    14641 aagcttatga ggctgcaaaa aatttcggga taaatgtttg tgcgcatgtg attttggggc
    14701 ttccgggtga aacaaaagaa gatatgctcg aaaccgccaa aaagcttgca caacttaaag
    14761 ttgacggggt aaaaattcac atgttatgtg cgcttaaagg gacaaaactt tctcaaatgt
    14821 atgaagaggg caaaatttcc tttatgagcg aagatgatta tattaatacg gtttgcgatt
    14881 ttctggaaat tttgcctccc gagactacta ttcaccgctt ggcgggaaac ggtttaaaaa
    14941 agaacttgat tgcgcctaag tggattggca agaagcttga tgcactgaac aaaatcgaca
    15001 gagaacttga aaaaagaaat tcttgtcaag ggggtaaatg tactccggct ttttgtatta
    15061 atcaaaaata aaatcaaagg agtaaatata ttccgtgata taatagggcg tatgaacaag
    15121 aaagttatca caaccaatag aaaagcatat cacgactata tgatttttga taaacttata
    15181 gccggaattg tgctgacggg aaccgaaatt aaatcaatca gaaaaggcgc gataaactta
    15241 aaagactctt ttgcaaaaat tgaaaacgga gaaatttttc tctataactg ccacatatcc
    15301 ccatacgagc agggaaacag gtataaccac gagcccgagc gtgtgcgcaa acttcttttg
    15361 aacaaaaaag aaattttaaa aatgctgggt aaaattaaaa aagaaggcta tacaatagtc
    15421 gcgcttgaac tttttttgac aaatgggttt gcaaagcttg aaatagggct tgcaaaaggt
    15481 aagaaacttc acgacaaaag agaagacatc gccaaaaaat cccaaaacag agatatgcaa
    15541 agggctatga ggggtaaatt agcttaacag gtgaatgagg tcggattgaa atactttact
    15601 tatttcaatg gcaatccaaa aaactttaaa aaaaaacttt ttgcgctata atattagcaa
    15661 tcaagatttt acaagggaaa aacagccatg atgtacagca aaaaggatat tttaagaagt
    15721 ttaaaagaac ttaattacta tatcgacgag caaattctaa acaattttat caaaaattgg
    15781 aaaatcgatg ccatatacga agacgaagat ggcgttgagt tctttgacaa tctttctatt
    15841 gttaaaatca aaaagggcat aagtcttaaa tcgcaaggct atgataatga cgaaattatt
    15901 tatcatatcg gtaaaatcct tgcagaaaaa cactttgacg aaattaaaga aaagcctgtt
    15961 aaagaaaaac ttattctttc aaacgaaatt ttgcctgcat caatttctgc aacagaggtt
    16021 aaaacaactt cgcttgatac tacaaacgaa aatttaaaaa tcttggctga cgttatagcc
    16081 caaaaagtta ccgaagaatt aagcgaaaga cttgataaaa tcgagttttc tcaaaacatg
    16141 aaaaaagaaa gcaagcttga gcaggataac gaaaagttat cccaaaaagt tgaagaactt
    16201 ttggaagaca acaaacaact cgcccaaaga gttgaacagc ttgaaagaag aaaaaattac
    16261 tttgtagaat tttttaaaaa atattttgtc ttttaagctt cttgccaatt aataagtttg
    16321 ttttttctga accacgtaag ttggcgtttt gcatagtgtc ttgaattttg ttttatcagc
    16381 aaaatcgcct cattaagact tatttttccg tctaaatatt ccaaaatttc ttgatagcct
    16441 atcgtgttta taaaattatt gattcgtccg tgttttttaa gcaaagcttt ggtttcttca
    16501 acaagcccct ttttaatcat ctcatcaacc cttaaatcaa tccttttgta taaatcggct
    16561 ctgtttgaag ggttaagccc aatccattcg acttcaaatt cgggctcttt tttgcctgcc
    16621 gctttggagt ggggcgattg aagtgtcaaa gacacttcaa tcgcccttat tattttaact
    16681 ttattattaa aatgaatctt ttgcgcactt tgtaggtcta atttttcaag catcttatga
    16741 agttttttgc tctccagctt ttcaagttct gccctcaatt cgtaatcggg ggcaactttt
    16801 ggcggggaaa aattttcaag caaaattctg aaatataagc ccgttccgcc tactacgata
    16861 ggaattttgc ctttagaata aatcttttcg atagcagctt ttgcatcatt ataaaaattt
    16921 gccacagagt agtcaatttc gggctcaacg atatcaatca ggtaatgttc tatgccttcc
    16981 atttcttcaa tagaaggttt tgctgttgcg atgtcaaatc ctttgtaaat ctggcgtgaa
    17041 tcggcagaaa ttatttcccc gttattcttc tttgctatcc gaatcgccag gcggcttttg
    17101 cccgatgctg tcggtcctac tattgctatt attttcttct tgtttaagga tttctctttc
    17161 ataataaaag aatattaaca caatgatata gacaataaaa tagaaatata aaagcattga
    17221 taagaaatac aaaattgaat taaatgccaa aatggcgttt ataaaagaaa caatgaagtt
    17281 tacaaaagag atgtaaagga ataaaattat tgctcccaga ggttttttaa aaattgattg
    17341 tatcgattta aaaagagcta taatcgggtt ttttgttcta taaattatct cgcatcccca
    17401 aaacattgtc aaaaaagatt gcacaaaagt aactccgatt agcatgaaat tccatgcgtt
    17461 aattttagca agctgttgtg ttgaaagcga agttataagt gctttcatct ctgcggtgga
    17521 ggtgagtgct cttttaattt gtacaaaatc aattcccggg tcgcctataa agtgcattcc
    17581 tgctttataa gccgcaatta caatcacaac aagtattgtg ccgtataaaa ttgcagcccc
    17641 cacaaaaggt aagaaaaatt caccgattcc gtagttaaag tcttttaaaa gattaaatga
    17701 agcttttgct ttttcttctt cgtcttcaaa tacttgattt gaaattttta cggcttgttt
    17761 tgtcataaaa agccagcctg caaaaaacgc acctatcata aaaagcactg ttactaagag
    17821 caaaatcgct gctgcaacat tttccggtgc tctttttgta aattctatgt aaaggcttaa
    17881 gataaataaa aacaacacaa gcggagtcgc aagaataatg ttttcgttag ttattttaaa
    17941 cgctttagaa aaatatttaa tcattttaca ccttagcttt ctgtttgttg ccttcttgac
    18001 gtttccgtct tctcattaaa tcaacaaacg cttctaatct tgtaatataa cctgctttac
    18061 ccgtttgttc atccataatc agagataaaa cggggatatt acaatcttct ctcatatttg
    18121 gaaaaatatt ttgcgacata atctcgggca tacaggtgaa aggacttatg tgaatcaagc
    18181 catctttgcc tttttcgctt gcccatgcga catcagatac acattcaagc gcatctccgc
    18241 cgatgtctct catcaagtag ggttttgcca ttctgaaggc tctttgaagg tgggtttcgc
    18301 cttttctgaa gatttttgga ataatggcat cttttaaaaa gctccctacg gtaagacttc
    18361 tgcgggtttg aacccccatt tttccaagtt ctctttcaat atgttggttt gaaaaataat
    18421 ctaaaaccag aaaaatttct cctgttatat caacatgtaa aatttctctg tttttgtcta
    18481 tttttgtttt tgctatttct tcaagtgcca tttttttagc ttgtttaagt tcttttgaat
    18541 ttgaggcttc aagaacgtat tttaatcctt ttttaaagtt tttttcggca tcgccgaaat
    18601 gattttccct tgccctgtaa taagaaaggg ctgtttccaa gtcgtcaagc acaaaaatgg
    18661 ttttaatagt tgccaaaatt gcccttataa acaaaatggg gtcgtttttg cctgttaatt
    18721 cttttgcaaa gttgtacata ccttttattc cgtcataaag tgccaattca atgtattttg
    18781 catcatatcc taaatcttta agattgtttc tgatactgct tccgtattca cctagcctgc
    18841 aaatgccggg agaggtaatc attgcaacat aatcggttcc gttttctatc gcttcaataa
    18901 aattccctaa aataagttta tagggaagac aaatcgcttc aggagaatat tttgtaccaa
    18961 gtgaaagcgt ttttctgtta gtataaggcg atacaaaagg ctctactccc atttttttaa
    19021 gccctgcaga ccatgcgatg taaattgttc ccatatgcgg aaacgctact ttaatttttt
    19081 gtttttcagt tttacttttt ttcattttac ctgttatcgg tgggatagta atcccaccct
    19141 acttattatg tcatcctgaa cttgtcttat ccttttgttt ccaaacaaag tgaggattgc
    19201 aagggagagt atcggtaacg atgtatcagg atctatctgg tgttgacccc tcaccctgaa
    19261 tttctaaaac tcgtacctcg ttttgaaatt catccctctc cctcaagggg cgagggggtg
    19321 gcttatgaac caatccttcg catttgcgtc gttgcgagcg actgaaagga gcgcggcaat
    19381 ccaagaaaat acttatttgg gattgctttg tcgtgcatat gcactcctcg caatgacaca
    19441 attattatac caagaacaaa agatttaccg catgttacgt attattaagt tgaattttaa
    19501 atcaccaaaa tccaatcata atgctgatta gattagatta gattagatta gattaaaaat
    19561 tacgtgtcaa ttttgagcag acaaaatgtt tttataaaaa tataaggatt taagagttac
    19621 attttaaaaa cgaggaggaa aaattatgac aatacagaca actgaatcaa tgccgattca
    19681 aagttctaat tatcggggtg ttccgccgat tccacaagga aggacaaatg aaaactatat
    19741 tcatagtatt catactacta ataatactac tactattata aataactatt atacggtacc
    19801 gactaaatca aatacaagta tgcataaaaa aaataatgat gttttaacaa taaatatttc
    19861 taagtcaaga gctaaggata caattttttt tatggtggca ccatttcatt atattacaat
    19921 aaaaggcgtt aaatgtttaa agaagctgtt ttaaataatt taaaaatatt aagagttaca
    19981 ttttaaaaac gaggaggaaa aattatgaca attcaaaaaa caacatttaa tgcaacacaa
    20041 caggctagaa taattaagaa agaaaagctt aattcgcttg aaaaaatggg gttgattttt
    20101 gcaggcggaa ttttgggacc aacattgctc ggagtcaatg aagttatgca tcccggctca
    20161 attaagaaag tagctaaaaa agcttatgaa ggtataaaaa atatttttta aaaataaatt
    20221 taaacaataa aaaagaggcg gacttaaaaa atccgcctct ttttttattt aaacttcact
    20281 ttataaatat tttcaagttc tctgccgtct tcatagtagt caagcccgta gcccaaaaca
    20341 aagtcttttt taattttaaa tccgacataa tccaatttta aatctttgtt ttcgggaaat
    20401 cttgcatcag gtttatctaa aagggtgcag atttttatat ccttgtcggg gtattctttt
    20461 gataatttgc ttaaaagcaa attcattgtt cttcccgaat caacaatatc ttctattatt
    20521 aggatattgt cataaaactc tattccgctt agattttttt ctttaaaatn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggcaagcgt ttcgtcgatt ttaccctttc
    20701 attaatttat caaaatttaa attgtgagag cgggcataat caataaattg tttttccttt
    20761 tctgaaactc ttattctaaa ttctaaaaca acacccgttt tgggcttacg ccctgcgtga
    20821 ggtcgtttgc cgccccattt gattttttct acttcttgga ctatttcttt ttgaatttct
    20881 tgagcctctt caggtgtaaa aaattctttt attaaatctt ttgttgttat ttttttcttc
    20941 attttaaata ttcctttttt aaataatttt caatattttt taaagcttgt tccatatatc
    21001 ttttaggtgc tttttgtgtt tttttaattg ttataaaacc aattataatt attttgtttt
    21061 catagtattt aaaatatact cttacgctat cgggctttaa ttcaaatagg ccatgaccta
    21121 aatcattaat gttcttatat ttagttccga cttgttcaaa aattgatatt gctttaatta
    21181 cttttctttt tttccggctt ttccggtttt tgaaattgtt ccaaggcttc gtttgtgtat
    21241 tctgctttaa attgttgcat ttttatcctt atttacattg tacttgattt tgtcccaaaa
    21301 atcaagtggg tttttaaatt ataattattt attccatctc aaatccggtt ggcgggcggc
    21361 aagcgtttcg tcaatttttt tgacaggagt tgtttttggc gattttaaaa cttcctgtga
    21421 actttgtttg atttcttttg cgattttaag cataacttct acaaactcat caagcctttg
    21481 tttattttcg gtctctgtcg gttcaatcat aattgcctca tgcacaatca gcggaaaata
    21541 aaccgtaggc gggtgagtat tttcgtccat cagccttttt gcaatccaaa gcgttgaaac
    21601 gccttgttct ttttgtttct cgcctgataa aacaaattcg tgcatgcagg tataatcata
    21661 aggcagttca taagtgtctt ttaatttttc ttttaaataa tttgcgttta aaacagcatc
    21721 ttcgcttacg attttaaggt tttttcctgt cattaaaata taggcataag cccttaccaa
    21781 aaccccaaag tttccgtaaa aacttttaac cttgcctatt gaatttggca gattatagtt
    21841 tcttatatat ttatccccgt caaattcaat tattggaata ggtagaaaat cttttaattt
    21901 tcccacaact ccgataggac ctgagccggg accgccgccg ccgtgcggtg tggcaaaagt
    21961 tttgtgaaga tttatatgta ctacatcaaa tcccattaat ttaggattgg tgtatcccat
    22021 aatggcgttg aaattggctc cgtcatagta taaaagcgaa ccgtttttgt gcattaaatc
    22081 cgaaatttcc aaaatgtttt cttcaaaaag ccccaaagta ttaggatttg tcatcataat
    22141 agcagcgaca ttttcatcca aaacagcctt gagcgcgtca atgtcaactt gtcctttttt
    22201 gtttgatttt atttctacac attcaaaccc gcacattttt gcacttgcag ggtttgttcc
    22261 gtgtgcagaa tcgggaataa taactttggt gcgtttttca ttgcggcttt caaaatattt
    22321 tttgataatc atcatccccg caagttctcc gtgagcaccg gctgccggtt gaagcgaaat
    22381 tgaatccatg cccgttattg tttttaaggc gcactgaagc tggtacatca gctccagtgc
    22441 gccttgtgaa tcttcatcgc tttgaagagg atgtaagttg gtaaatcctt ccaagtttgc
    22501 taaaaattcg tttactttgg gattgtattt catcgtgcaa gagcccaagg gataaaaccc
    22561 tttttcgatg cagaagtttt tgtccgaaag ctctttgtaa tgccttagtg cttcaagttc
    22621 gcttacttgt ggtaaattaa gcggggtgtt tcttaaaaat tttttattta aaaagtcagc
    22681 tttttcttca atatctttga tatttattcc taagttattg cttttttcaa aaatggtttt
    22741 tgtcattttt tttcttctta aactacgtta tttttaatga ggtctttttt aactttgttg
    22801 agccctactt ggattattct tgagattctg gaaggtgaaa tatcaaagtc gtgtgcaata
    22861 tcttttaatt tcttgtcttt aaagaatttg ctttcaatta aatctcgctc tctttgagga
    22921 agttttctta ttgctgcgat aatttcagct ttaagttcaa agaactccaa actttcttca
    22981 gggttttttg attcatcttt aatgtgctta ggattttccg catcagccat ttcatctatt
    23041 gaaagaatgg ttttataaag ggcttctctc aggtcttctt gttctgtttc atcgcattct
    23101 tttttgtttt taagagaata ccatttgtaa cggcgtctga cttcatttct tatcgcccat
    23161 tttatagcag ttgaaaggta agaagtgtta aattgctcgg gattattatt tttgaacagc
    23221 aaatagaggg tatgtgtccc gatatttact atttcggagt aatctatcaa gtgcgcacag
    23281 ttaagttttt gatactcaac ttttgcaata acctcaatta atgttttgta atctttaagc
    23341 ctagtcttaa ccgctttttc tgtatgcata aacctaattt tccctaacag cttaattcta
    23401 tactaaaaaa aaatagatta ctagacaatg tgctttttat taacttttgt tatcttgtta
    23461 gaattaaaaa aggagaaaat taaaatgaaa gctttatttt gtaccgaagg ttctgatata
    23521 agtttattgg cattggaaaa tgtttcaaaa tatcttaaaa atattgtttt agatataatt
    23581 tgtgtaattg attggagttt tttacctgat tctatgaata tagataggga aacttattcc
    23641 aggacttacg aaagtattgc agatagcgtt ttgaattttg ccgaagatgc gattaaggaa
    23701 aaaggtctta gtgtcgggaa aaaagttaaa aatttcggct caccggttga gggtatttta
    23761 gaacaggtcc aaaatgactc ttatgattta attataatgg gctcgcatgg taaaaaaggg
    23821 cttcaaaaat ggttgggctc tgtttcaaga caagttttga caaatgtttc aatgcctgtt
    23881 tttatcagta aaagaaaaaa tgaaggcaaa aaaatactta tagcaataga cggttctgaa
    23941 atttcttatt ctgccgtcaa gcaagctaca gaactttttg attttacaaa taaagaaatt
    24001 catgttgtta gtgttaaaga aactcctgaa cttttgccag tggaagtggc tctagataaa
    24061 aaatggattg atgatattga taagcaacaa aaaattcatg cttcaaaggc aattaataaa
    24121 gtaaaatcaa ttttggcttc aagtaatttg tctgttataa atgaagttat tttgacagga
    24181 catcccgcac ataaagttat tgaatttgca caacaggaaa atattgactt gattgttatg
    24241 ggtgcaagga caaaagcgtc gctttcgact tttttgttgg ggtcggtgag caagcgtgtg
    24301 cttgaaaata ctaacagcga tgttttgatt gtgaggaaat agtggataag ttcatttata
    24361 atttaaaaat tacgtcattc tgagcactct gccgacaaaa cgattaagta tcgttttgga
    24421 gagggcgaag aaccttgtgt cgttgcgagg ctttagccga agcaatctaa aaagtattaa
    24481 attttagcat tttttgaacc tactttcttg taccgacaag aaagtaggca aagaagctct
    24541 ccgacctgat ctgcattcgc taatttacgc taaactcaac ccgagctcgc gaactcgtgc
    24601 ctgcggcact caagcacact cgctttgtcg ttgtttcgtt aagtgtaaat tgctcactcc
    24661 gatatggtcg gaaaaatact tgatgctatt ataattaaca ttacaaatat aaatattatt
    24721 ttcgcaaaaa aagacctcat atttttatga ggtcaaggtt ggttattttt ggtttattat
    24781 ttggttatta ttggtttttt atattttaga taattattaa atatccctca aggttaaaaa
    24841 ttgctagcaa atttgtacaa attttttaaa aaccgccaaa attcaatgat aacaagaaat
    24901 attcaagtta acatttctta aacattttgt gttttaaatt tctcaataat gtgatacaat
    24961 gaggacaagt atttatattc ggagatgaaa aatgataaat aaagcaagaa gagcggataa
    25021 tattat
//
LOCUS       DAOQAU010000022        24606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2033_length_24606_cov_7.869659, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000022 DAOQAU010000000
VERSION     DAOQAU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 24606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24606
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_24606_cov_7.869659"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(446..2110)
                     /locus_tag="PKI94_04765"
     CDS             complement(446..2110)
                     /locus_tag="PKI94_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW26085.1"
                     /translation="MNKFEFFSKFNVPVIIVNEKKEVLYKNHVFLRTFSDFVDLEKFS
                     HQLNFDVCVLEEGNIEAYSPIYQAISSPQNFFARVICQNSGKENFYYNITSIKRNRYV
                     VIIFDDVTAQNKLESLEKEHNKLSKDYKQLYEENQNFVKIKQKAQAQAIKMVLINKMS
                     NIIRESIDISKVINSALKELSVMFGAFKVYYASSINKSFKKEEILPLEFKPEIGSIIK
                     FDDATYKSITNKQIHVSNCIKECLNSNETLKEKASRIIVPIYNLNQFLGIIVILSNQK
                     RELNDEIGILESVSAQLGTAIVQASLFEQINKQRDELEIALKELKDTQLQLINSEKMA
                     SLGQLIAGVAHEINTPLASISSNNEIIAKVIKKIGDENVSEMLKEVNQLDKEAVQRIS
                     NIVKSLKKFVRLDEAELQEADINHELDLTLDLIRHETKNKIEIIKHYGVLPLIKCYPN
                     MLNQVFMNILINACQSIENKGSITITTSFKKNTLTVSIKDTGKGMNKEEINKIFTAGY
                     TTKGVGVGTGLGLAISEKIIQKHNGKIQVNSEIGLGSEFIITIMGK"
     gene            2329..2898
                     /locus_tag="PKI94_04770"
     CDS             2329..2898
                     /locus_tag="PKI94_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW26086.1"
                     /translation="MPEYLSKEEIKRWRSSLEKITLEEYAARLGKVIAEEKHTNDIVD
                     IVLKGQPVMSRFDNLAQTRGEKITSIAQRAFEREKELSKSSSKTDSIIKKTIAKQANA
                     QKQEQSEAPKNLKEEVRLVFNKALTDREQAVFDYFAQNNNKIVYAKDLAKLLDLPRDY
                     VYKYIKNLRTKIDGDCLKNADNGGFVLSV"
     gene            2981..4189
                     /locus_tag="PKI94_04775"
     CDS             2981..4189
                     /locus_tag="PKI94_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNW26087.1"
                     /translation="MDSTIIGILEILSAAKKLSSVGFSAGYNFSNEKVEEYLNELLEN
                     INNNDPIFSGVIIIEKSEKVGNFIIIDGLQRITTVCLLLCALCAGYKDTSAKNNEARY
                     KIFTRYLINEGEVKLQLDGGWGEIYRRIVFSLEFTEREKKNSLYRTYKSFLDTIKIKK
                     ISATKLFDLIKRMKFMVIFTEKTEIPAQEIYQSLNPTKEDLSQINLITSFISEQDEAA
                     ENIWGKTVESFTGIGLSNSLKEFIKDFLTIQSNGRVPEKNELYKYFKRYYKKISQYQS
                     AQQITEYIYRYSKFYLKIIQANFDDFEIQKHIVAINENNGQDTYPYLMEVLDDFENQC
                     IKKEMFLEILMMVNGFVTNRRENPTEDKINFASLSREINKTLALQQFQPSEKQVEDRY
                     KEKIKLKDMN"
     gene            4239..6119
                     /gene="ftsH"
                     /locus_tag="PKI94_04780"
     CDS             4239..6119
                     /gene="ftsH"
                     /locus_tag="PKI94_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNW26088.1"
                     /translation="MNKSQSMGMYVILGIMVLAFVSMLVAGPNTSTQELSYTDFLQKL
                     KAGDIKNVEIVDEKSLVAHPKVQPPMAKKESSETLKALYGEQKAPVLQYKVLTPSDPE
                     LMKKLEAANVEINVKKPSESSQWMNILGSLILPILFIVFLLIMAKSLQAGGSQAMSFG
                     KSKAKMMLDNKVKITFKDVAGIDEEKQELAEIVDFLKNGEKYIKLGAKIPKGVLLLGV
                     PGTGKTLMAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFDQAKKHQPCIIFID
                     EIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDENTNIIVIAATNRPDILDSALLR
                     PGRFDRQIVINRPDILGREQILQVHAKNKPLADEVDLKVLAKRTPGFTGADLKNLLNE
                     AALLAARHDKVKIQMDDLEEAIDKVMAGPEKKSKIISDEEKENTAYHEVGHALLSKLL
                     KDCDPLHKVSIIPRGMALGITMTLPEKDHLTLKKNQLIDRITMTLGGRVAEELIYGKD
                     FITTGASNDLEKVTALARKMVTSYGMSDKMGYMAYGKSEEHVFMGRDFGHTRDFSEEI
                     AAEIDKEVKRIVDDCYNNAKKLLEENRDMLEYIAKTLLEEETLDEKEFVELMDKVKSE
                     RG"
     gene            6127..7050
                     /locus_tag="PKI94_04785"
     CDS             6127..7050
                     /locus_tag="PKI94_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26089.1"
                     /translation="MSSEQSVIRQINFQGLKIGGEGVNVTDIYFSTSHVVEAGKNDTA
                     CPYFALELNIFDFDASSHIVKDYYGEKKPIKLVQKAQESNCDILALKFNISEENVDEN
                     ILRAKTLLAELLPHIKKPLIIRGMNNKSVDVKLLPALMEVLDREVIVAFADENTYKEF
                     VPPVVKGGHILVIRTPIDINLAKEMNILTTDMGLSPDRILIDTDMGGLGYGLEYGYSI
                     MERIKLAAFDGDEMLNMPLIAFAGEEALKAKEAKSDTFSKSFGDFKTRSLMYEITTAS
                     AVIAAGANIVVLEHPKSVEILNKLLRHAEFI"
     gene            7242..8462
                     /locus_tag="PKI94_04790"
     CDS             7242..8462
                     /locus_tag="PKI94_04790"
                     /inference="COORDINATES: protein motif:HMM:NF015556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNW26090.1"
                     /translation="MTELSGLQIFKYLPAAKKLSEGNCKKCGLPTCMAFALKLAKGQA
                     QISLCPYVSKDLKEMFELGMKKPQKEILLGHKNKVLVGGENVLYRHDKTFVNKTAVAV
                     VIDTKEKDWQNKLQRVLDFEITRVNETFKVDLIYLKGKYPPPQSSPSRGGEDMSFSKL
                     TDKLDDLGFAWIVEEDLKDLIKVEDKGVFETVEKLTKIRKKAVLEKDDKYSTPVYVYF
                     PSPLGGEGGQSPGEGLLELMSKASFYLCKYANMLIFEDFDEALFSTLMTLRQNIFTDP
                     QKPLQVESKVYEFNNPDKNALIFLTTNFALTYFAIANEIEALNKPAYLVVTPSEGMSV
                     LTAWSAEKFTAQMVAKTVAKYELAKKVKNKKIIIPGLLAHMAQELREELKGFEIIVGP
                     NEAYLLADFVKSIN"
     gene            complement(8455..10863)
                     /locus_tag="PKI94_04795"
     CDS             complement(8455..10863)
                     /locus_tag="PKI94_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNW26091.1"
                     /translation="MIRDYINSAAQKKYFLMFFTSVIYILGLFAYFSQKSIEFSFVLL
                     IIAIFAILKNYISPKLALFWYFVFFFAFFNANLRIKNYDTLYQIAPKDAVITGQITSI
                     PSGINGREKFFFNVKKISYDKKEVEIKAKTYINMIPCFERSRNKCGMTLNIGDTYIIK
                     GKLRPPFKASNPSQFDYGKYLKNFGVFTLFYAQDADFKKLDTKLSPKWAFLQGLNNIR
                     NQILTTHAKYLKSPNLEILGGIVFGDDAVAPPDYIKKAFINSGLLHILAASGMNVAII
                     YGIWFFILRKLKVPFNITVSSGIFVIIFYALMTGLGPSVIRAAIMLIFVLLGKLIDRD
                     AHSLSLLSFVALLMLIYNPAYINDVGFQLSFLVTFGLLTTAPVVFERLKRVPNWLSGA
                     VFIPIVAQIWIAPLQMYYFNSFSLYSVFANIAIMPFVSIVSFGGFISSVIAMFKPVAD
                     ITCFVFDSALNPVLNALVAISQYFSNLPHSLVITPQPNDFQMILYYFLVVLITLLFKF
                     GREKRIITATIISLAVLFILYIPLPDKDFETIVFDVQNADAFLIKTPKNKYFIIDSGK
                     QGFKGGKSQADFIILAYLKDKGIKNIEGLILTHFDSDHAGGAVDLIKNLGVNAVYLNS
                     LDDKSPLAQKIYNLAKNKKVKTFAVKNNEIIYFEPDFKIKTFISGIKNSDNENSVITL
                     VSRGDFDELFMGDAGVEAFGKIAPYMPDIEVLKVGHHGARNVVDKKMIEKLKPEAALI
                     STGPNHYGHPSGVTLDVLQSQNVKVFITEKQNSLKVENSKNGYKIKSYDSRNGKYIEK
                     SGFN"
     gene            11029..12420
                     /gene="cysS"
                     /locus_tag="PKI94_04800"
     CDS             11029..12420
                     /gene="cysS"
                     /locus_tag="PKI94_04800"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNW26092.1"
                     /translation="MKLYNTYSQKTEEFISIEANKVKMYVCGPTVYDLAHLGHARCYI
                     TWDVLYRYLKFKGYEVTYCRNVTDVDDKILNKAQKEGKTPDEISQHYYKLFSESMKKL
                     NTLPPDIEPFATKTLGEMIAMNKQLIEKGFAYESNGDVYFRVKKFDKYGALSKQPLDA
                     LEAGARVETSEIKENPMDFALWKKDEKFGYKSPWGTGRPGWHIECSAMSRKYLGETID
                     IHAGGADLIFPHHENEIAQSECANGCQFVKYWLHNGFVTINKEKMSKSLGNFLTIDGI
                     LEKYDANTIRFFILTNHYRMPVEFSDEALESAQAGVKRLYNAINQSRKTVKIDENLNM
                     EAFDEYKQFVEAMDEDLNTSKALAVLFDLANKLNKVPNPQNLALLINLSEALGFNFDK
                     VEISQDELKEKINLISSNLGKTFADMEEILQYRKSAREEKNWDLADKIRLAFDEAGIV
                     LKDSKDGTTWELK"
     gene            12405..14387
                     /locus_tag="PKI94_04805"
     CDS             12405..14387
                     /locus_tag="PKI94_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNW26093.1"
                     /translation="MGTEIRHKTPKNQRHAELVSASYKRYALAFLLFIIFSFGLKAFA
                     GGVFSINTDLKVPFVSSEKKNTEKSEILETPGVPQKAATPTPPTTSTPPAQPVEKTSP
                     PPETAAKTEQNKIILPPTPAIQQSLSLNDNALVRVALTNNNFSSYAWGEASILATSDF
                     SLIEKESGKEIAKFLPADVVKIKMNSGVLEVKLNDKPVVKTASALIFTCPKGFLGIEG
                     LKRNSRQAIYRGNFEITPKSPTQFYIINVVDVESYLKGVVPNEMPVRFGLEALKAQAV
                     AARNYVLTPRTRSCKEFDVDDSVASQVYFGAGTEDLLSERAVDETRGLVALYGWDLIV
                     AQYSSTAGGYTESYENVFSDPKTKEFPSKSRPYLVGTPDIIGVLPLDSEEAARKFYMT
                     APDSYDMKSPYYRWQKVWEGTDELKNVLQQTLVVQSKTGFVKPEFKKGDILGEIKELK
                     VNKRGVSGKAMELEIVTDKQNYKIYKELVIRRLLTKEGKALPSANVVFDHIWDENGKL
                     TKVTAYGGGYGHGVGMSQFGAGFMATSLNKSFDKILKRYYQGITIATVPLILSDSQNQ
                     HVGIQKFYAPKQSATVVVDNKYQIKQFSAKINSRDVTFELANSFIPIHRYSRIDISSY
                     IRRGKNEIIFYFPQNCENKAIRVYVELVEKSEGYEF"
     gene            14384..16009
                     /gene="murJ"
                     /locus_tag="PKI94_04810"
     CDS             14384..16009
                     /gene="murJ"
                     /locus_tag="PKI94_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNW26094.1"
                     /translation="MSTQENVSAENEKGPSLLKAAWLIAMVTVVSKLIGFIRDVVIAN
                     FYGASMVSDAYFYAYQIPALAIILLGGVGGPFHSATVAVFSKLIPSFKEKANEQLNKL
                     FNTFLTASFLFFMVLAVLCFVFSDQIMGFIISGTNPELTALAGMHLKIMSPVLIVGGI
                     VGIYYGILISYREFILPNVSPILMSVVIILMISAVKGDNTGSVLAWATTIGALCQFVY
                     QLPKIKQLGYRLRPNFDFYNPQFKNICELLFPAILSSTVGQIHIYVDMFFASSLKEGA
                     WTAIGYANRVFQFPVGILVTAFLVPLFPIFSRLVAQEDFEGIKSYFNKGVGVLLFAAV
                     PIIIGILTLGYDGIRLIFQRGAFDDNATFMVTQALWFLSFSILPYVFRDSITRVYYSF
                     NDSATPFLVAFSSIILKFFLNWLLVAKLSIGGGIGGITLSTTLVTLFNAVVLGVLIGR
                     KIKLDYKSLFSNFAKMFTAGAVTLFVCWAMCIWFDTLMLPRTIFEISKILFVTSICLV
                     CYIYLNLLYKMDYAIELKERFIGKMSEKYGLFK"
     gene            15993..16589
                     /locus_tag="PKI94_04815"
     CDS             15993..16589
                     /locus_tag="PKI94_04815"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNW26095.1"
                     /translation="MAYLSNQLKQKIKNTLDNDGVIAFVTDTVWGLGCLPSSKKAVKK
                     IYEIKKREAKKPLILMSNSVENLLLYVKSLSKKANELAEKYFPGALTLVLKKSGKTPD
                     FITSGFDTVGIRIPDNKVFQEICECIEGKVLATTSANLSSEPSAKTYKQALEKVGNTA
                     DLVIEDFGQLAKGLESTVALILENEVRVLRQGAVKFDI"
     gene            16730..17107
                     /locus_tag="PKI94_04820"
     CDS             16730..17107
                     /locus_tag="PKI94_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26096.1"
                     /translation="MVDNRAAGFFGIGGAFNFKSGDISGTAAKTQDDKMGPGTEYYAQ
                     GQNEEEQNQYEFPFIDRSAQLRATLNSLAMMNVANVLNSKKRTTFLEDMEELEEEKEY
                     EENEEFMDDFDEDNYEEDGQEYY"
     gene            17258..19150
                     /locus_tag="PKI94_04825"
     CDS             17258..19150
                     /locus_tag="PKI94_04825"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNW26097.1"
                     /translation="MGCKLADKNLTSKVVQEALKALDKKNFALIAHSSSFPSLSDEDT
                     GFGSINSAAGKNLIDFASGVFNAIQLGPAGKTKACDSSPYTGTIFSGNPLFIDLKQLT
                     TKQWNNILSEETFNSVVQNNPNKNSGKTAYSYIYKAQQSALKEAYENFKNSASEKIKE
                     EFEKYKRENNTWLEKDALYEALSIENCNDYWPLWQSETDKNLLNPKSIEEKMEFANRI
                     NEIEANYEDEINFYKFCQFVLSVQNSQTKEYALSQGIKLIADRQVAFSDRDTWAYQSL
                     FMEGWFLGCPPDYFSKDGQSWGFPVIAPEKLFKPDGSLDEGGVLMKELFKKMFKENPG
                     GVRIDHIVGLIDPWVYKAGKNPKVEEGAGRLYSSPEHPELGKYAIPSLNDLNHDLEPD
                     KEKRVQTLSDEQIKLYGRIIEHIVIAAAKEEGLDKDSIVCEDLGTLTNPVAAVMEKYK
                     LQGMKLTQFVSPEMQDHPYRCRNIDKNSWAMVGTHDNEPISMWAENMINTHEGYLHAK
                     NLVEDLFPDEENKDDIIMNLTKDLTFLAQTKLVEIFASPAQNIQIFFTDYFGIKDVYN
                     RPGTSGDGNWNLRIPNNFEEVFCKNLKEQKALNLALTLKKAIEARGNGFVEKHKKLLG
                     ELNKLI"
     gene            19161..20015
                     /locus_tag="PKI94_04830"
     CDS             19161..20015
                     /locus_tag="PKI94_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26098.1"
                     /translation="MKKILVLLLILISTSLFATAQEANTEAVLQYNQGIDYYKAGEYD
                     KSTTAFKTAISLDPDYIDAYYNLGSVLEFLGKYEEALVIFKQVVVRKPDDYDSVYKAA
                     WLSKQLGQNQKALTYLSIIPIDCERAKDAQVLAQEIKQQGTVQNIDVSAPIKEVKISE
                     PKISQSNFVYQNILAPTGITSDSEGNLYVAEFNNNLITKITPDNKKIIFVKDSKISGP
                     IGLATDKTGNIYVANYNKDNVLKISVFGEITVLIGNLKKPYYLYVKDNMLFVSCQGTD
                     TVLRYKLN"
     gene            20435..21331
                     /locus_tag="PKI94_04835"
     CDS             20435..21331
                     /locus_tag="PKI94_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26099.1"
                     /translation="MVDSINNVNSSVIGQNYKKPSFWNYAGGVAAGSLISRLPSVLLN
                     QVSMLSEKKYKKLIQLNADEFKNVQEAVNKTFSSSRLSEKGVSILEVTQKNIKEVTEA
                     IKATIDKNMRFRSKEFREMWTKIEVETTMSGDAAQYLTESKKILFDKKSLMSAFHEIG
                     HAVNGNLSKMGKIMQKCRSLSFLAVPIALIALLKNKKSPDEKPKNKIDKFTDFIKRNA
                     GKLTFISALPMLAEEALASIRGNSFAKKALSPDLAKKVAKANFAGFCSYLGLVVASSI
                     GIFLGIKVRDAIVKPKNVEDKK"
     gene            complement(21332..22198)
                     /gene="metF"
                     /locus_tag="PKI94_04840"
     CDS             complement(21332..22198)
                     /gene="metF"
                     /locus_tag="PKI94_04840"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNW26100.1"
                     /translation="MTLKEHYSNSKKPVISFEIFPPKDDETKEKFQKLLGHLKTLKKY
                     NPAFVSLTYGAGGSTQTNSFDMIKKIQSEVGLNVMPHFTCVCVRKNHILEYLSQIESL
                     GIKNILALRGDVPCDCSVKNFDFHYANELVEFIKTHSNLSIGVAGYPEGHLECDNFML
                     DMKNLKRKVEAGADAVFTQMFFDNEKFYSFCQSARDLGIEVPIIPGILPIRSYGQLEK
                     MLSMARVSLPEKLRIKLEKFKDNPDDIKKLGVEFATQQCQELLKFGICGLHFYTLNSS
                     KAPSEILDNLRG"
     gene            complement(22220..22450)
                     /locus_tag="PKI94_04845"
     CDS             complement(22220..22450)
                     /locus_tag="PKI94_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26101.1"
                     /translation="MENLLHYSPIIIAIMVFLIQQRIVVTPEQLERKHREILKDAEER
                     FATLNSVKDLKEQFSDMKEKIDKIYDCIIAVK"
     gene            complement(22462..23754)
                     /locus_tag="PKI94_04850"
     CDS             complement(22462..23754)
                     /locus_tag="PKI94_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNW26102.1"
                     /translation="MKYKLLTAQREFLEIPHDYSLDVAVYQGGFGSGKTFAGSLLGIL
                     LCLKFPGIRGLVGAQTYTLVRDTTLQSYFEHLENLNFKDGKDYFWSSSLQKLTFKNGS
                     EILFRHFDEPNKLKSLNLGFAEIEEMSDIPFSTFKMLLGRMRQKIKQGWKNFTYRIFG
                     HTNPEVQRGWIYKTFVDEHKPNYRLISAPTTDNIYLPEGFCEELKKLYDEQYYRIFVL
                     AQNGEYDKGLIIKDFTDENIKEIKYQQDMDLHITCDFNVDPMAWILAHKTQDKVFFFD
                     EIVIEDTTTAKACDEFYHRYPNHKGRIIINGDASGDNRSCTSEFTNYVIMKKKLLQFG
                     YEDVEIRIKAFNPPIKNRIAAFNAKVHGADGKIRLFTDKKCEKLLYNIYNLRYKEGSS
                     KIDLPSYQQIKNSSELKFLGHPMDAASYLVEFYWPISI"
     gene            complement(23861..>24606)
                     /locus_tag="PKI94_04855"
     CDS             complement(23861..>24606)
                     /locus_tag="PKI94_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26103.1"
                     /translation="NPSTGRGLSPLRVALILNNISSTILNKQVDALALMMNPPYLAPK
                     GCFKGEQVVKPGKIIEYDSSLMPTAPSPLSFEKALHGWDFLNYFKTTIESATGIFKNM
                     AGNIQTAQRTATELNYSVSGQTTRLNMMLDAINRKIIIPMVEKTAELIANFKIGREVI
                     SVFDQGKTLFIEIDDTIRNAKYIYRYGDRKATLERKLRSKELFDVIRAFAEVPTMTNQ
                     INWIECFKFALEQYGIENANNFLVESNGN"
BASE COUNT         8080 a         4224 c         4799 g         7503 t
ORIGIN      
        1 tacgtcaata agcggttcaa ctgcgtttga atcgcccatt tctcccaaaa tacgtgccgc
       61 attatatctt actttttcgg aattgcttgt ttttaagtca cacaaaagtt cttcaaatgt
      121 cttttttact tgttttttct tactttttac actgattgtc attcgttttc ctccgacaca
      181 aaatattaac tacaccacat aatttattaa aaaaatccgg tgagaaaata tttacttttt
      241 acgatttatt ataaagtttt tgtaacattt tgtttgaaac tcattattaa gggtaaattt
      301 aacactttat agtaacataa taacatattt tttgagcaat tacttacgcc atgtggatta
      361 tttttcgttt tgtatgattg ttttaataat ataatctcta attcaaaaat agattaaagt
      421 aaaaattaac aaaacttaac acttattact tacccatgat agtaataatg aactcgctac
      481 ctagcccgat ttcactattt acctgaattt tgccgttatg cttttggata atcttttcag
      541 aaatagccaa tcccagccct gtaccaaccc ctactccttt agtagtatag cctgcggtga
      601 aaattttgtt tatttcttct ttattcatgc cttttccggt atcttttatg ctaacggtta
      661 atgtattttt cttaaaagag gttgttatgg ttatagagcc tttattttca atgctttggc
      721 aggcattaat taaaatattc ataaaaacct gatttaacat gttaggataa cacttaatca
      781 gaggaagcac tccataatgc tttataattt caattttatt ttttgtttca tgtctgatta
      841 aatccagtgt aagatcaagt tcatggttaa tatcggcctc ttggagctct gcttcgtcta
      901 atcttacgaa ttttttaagg gattttacta tgttgcttat acgctgaact gcttctttat
      961 caagttggtt aacttcttta agcatttccg aaacattttc atcgccgatt tttttaataa
     1021 cttttgcgat tatttcgtta tttgaactta ttgaagcaag cggagtattt atttcatggg
     1081 caactccggc aattaactgt cctaaagatg ccattttttc agagtttata agctgtaatt
     1141 gggtatcttt aagttctttg agtgctattt caagttcatc gcgctgttta ttaatttgtt
     1201 caaacaaaga tgcttgcacg attgctgttc caagctgtgc cgaaacactt tccaatatgc
     1261 ctatttcgtc attcagttct cttttttgat ttgaaagtat tactattatc cctaaaaatt
     1321 ggtttaaatt ataaatcgga acgattatgc gagaagcctt ttctttaagg gtttcattgc
     1381 tgttgaggca ttcttttatg caatttgaaa catgaatttg cttatttgta attgatttgt
     1441 atgttgcatc gtcaaattta attatagagc ctatttcagg cttgaattca agcggcagga
     1501 tttcttcttt tttaaaagat ttattaattg atgaggcata ataaacttta aaagccccga
     1561 acattaccga aagttcttta agtgccgaat ttattacttt tgagatatct attgattctc
     1621 ttataatatt agacatttta tttatcaaaa ccattttaat tgcctgagct tgggcttttt
     1681 gtttaatttt tacgaaattt tgattttctt catagagttg tttgtaatct ttagaaagct
     1741 tgttgtgttc tttttcgagg ctttcaagct tgttttgcgc tgttacgtcg tcaaaaatta
     1801 tcaccacata gcggtttctt tttattgagg ttatgttgta ataaaaattt tcttttcccg
     1861 aattctggca aataactctt gcaaaaaagt tttgaggaga tgaaatcgcc tgataaatcg
     1921 gcgagtatgc ttcaatgttt ccttcttcaa gaacgcagac atcaaaattt aattggtggg
     1981 aaaatttttc caagtcgaca aaatccgaaa aagttcttaa aaatacgtga tttttataca
     2041 aaacctcttt tttttcgtta acaataatta ccggaacatt aaatttagaa aagaattcaa
     2101 acttattcat aattatatta tacagtgaac agtgagctgt gaacagtgag cagtaaaatt
     2161 cttttcactc tccacttctc acctctcacc attcaccgtt tacttttctt tacattgtaa
     2221 agtatttaaa acaaaaattt acgttatgaa atattttgtt tcacaaaaaa agttttatga
     2281 taaatttata aaatataaga tttgaatttt tagtcaagga gatattgcat gccagaatat
     2341 ttgagcaagg aagagattaa acggtggaga agctcattag aaaaaattac actggaagag
     2401 tatgcagcga ggttaggtaa agtaatagcg gaagaaaaac acactaacga tatagttgac
     2461 atagttttaa agggtcaacc cgttatgtcc agatttgata atttagctca aactcgcggt
     2521 gaaaaaataa cttctattgc ccaacgagct ttcgaaagag aaaaagagct ttcaaaatca
     2581 tcttcaaaaa ctgattctat aatcaaaaaa actattgcaa aacaggctaa tgcccaaaaa
     2641 caggagcaat cagaggctcc aaaaaatctt aaagaagaag tcaggcttgt atttaataag
     2701 gctcttaccg atagagaaca agcggttttc gattactttg cgcaaaacaa taataaaata
     2761 gtttatgcaa aggatttagc caaattgctt gacctgccaa gagattacgt ttacaaatac
     2821 attaaaaatc ttcgaacaaa aatagacggg gactgtttga aaaatgccga taacggtgga
     2881 tttgttctct cagtttaatc attttttaaa ataaatattt tcaagaaaaa aacacgtgct
     2941 ataatatatg taataacacg tgttttttat gggaaaaaat atggattcaa cgattatagg
     3001 catactggaa attttaagtg ccgccaagaa gcttagttcg gtaggttttt ctgccggtta
     3061 taatttcagc aatgaaaaag ttgaggaata tttaaatgaa ttgcttgaaa acataaacaa
     3121 taatgacccc attttttcag gtgttattat aatagaaaaa tctgaaaaag tcggtaattt
     3181 tattatcata gacggtcttc aaagaattac aaccgtttgt cttttgcttt gcgctttgtg
     3241 tgcaggatat aaagatactt cggctaaaaa caatgaggcc agatataaaa tttttacacg
     3301 ctacctgata aatgaaggag aggtaaaact tcagcttgac ggcggctggg gtgaaattta
     3361 cagaagaata gttttttctt tggaatttac cgaaagagaa aagaaaaaca gtctttacag
     3421 aacatataaa agctttttag acacgataaa aattaaaaaa atttctgcca caaaactttt
     3481 tgatcttata aaaagaatga aatttatggt tatatttacc gaaaaaacag aaattcccgc
     3541 gcaagaaatt tatcaatctt tgaatccgac aaaagaagat ttaagtcaaa ttaatcttat
     3601 tacaagtttt atttcagagc aggatgaagc tgcagaaaac atttggggca aaacagttga
     3661 gtcttttacg ggaattggtt tgtcaaacag tttaaaagag tttataaaag attttctaac
     3721 tattcaaagc aacggcagag tccctgaaaa aaatgaactt tataaatatt tcaaaaggta
     3781 ttataagaaa atttctcaat atcagagtgc tcagcagata acagaatata tttacaggta
     3841 ttcaaaattt tatttgaaga taattcaggc aaattttgat gattttgaga ttcaaaaaca
     3901 cattgttgca attaacgaaa ataatggtca ggatacttat ccttatttaa tggaggtttt
     3961 ggacgatttt gaaaaccaat gtatcaaaaa agaaatgttt ttggagattc ttatgatggt
     4021 aaacggtttt gttaccaaca gaagagaaaa tccgacagaa gataaaatca attttgcaag
     4081 cctaagtcga gaaatcaata aaactcttgc cttacaacaa tttcagccgt ctgaaaaaca
     4141 agttgaagac agatacaaag aaaaaataaa actcaaagac atgaactgat ttttttaaag
     4201 tataataatt cagaagagac ataaaaacag aggtaataat gaataaaagt caatctatgg
     4261 gaatgtacgt aatcctcggg ataatggttt tggcgtttgt gtcaatgctt gttgcaggtc
     4321 ccaataccag tacgcaggag ctttcgtaca cggatttttt gcaaaaactt aaagcaggtg
     4381 atattaaaaa tgtcgagatt gtagatgaaa aatctctagt cgctcatccc aaagttcagc
     4441 cgcctatggc taaaaaagaa agcagcgaaa cgctaaaagc attatacggg gagcaaaaag
     4501 cacccgtttt gcagtataaa gttttaactc caagtgaccc tgagttgatg aaaaaattag
     4561 aagcggctaa tgttgagata aacgtaaaaa aaccaagcga atcttctcag tggatgaaca
     4621 ttttgggttc tttgattttg ccgattttat ttatagtttt tcttttaatt atggcaaaaa
     4681 gccttcaagc gggcggctct caggcgatgt ctttcggcaa aagcaaggca aaaatgatgc
     4741 ttgataataa agttaaaata acattcaaag atgttgcggg aatcgacgaa gagaaacagg
     4801 aacttgcaga aattgtcgat tttctcaaaa acggtgaaaa atatattaaa ctcggcgcaa
     4861 aaattccaaa aggcgttttg cttttaggag ttcccggaac aggtaaaacg cttatggcaa
     4921 aagctgtagc gggcgaagca ggcgttcctt tcttttcgat aagcggctct gactttgtgg
     4981 aaatgtttgt tggtgttgga gcaagcaggg tgagggattt gtttgatcaa gctaaaaaac
     5041 atcagccttg tattatattt atagacgaaa tcgacgcggt cggacgtcag cgcggagcag
     5101 gattaggcgg cggacacgac gaaagagagc agactttaaa tcagcttctt gttgaaatgg
     5161 acggctttga cgagaatacc aatatcattg taattgcggc aacaaaccgc cctgacatat
     5221 tagacagcgc acttttaaga ccgggcagat ttgacagaca aattgtaata aaccgtcccg
     5281 acattttagg cagagaacag attttgcagg ttcatgcaaa aaataaaccg ctggctgatg
     5341 aagttgattt gaaagttctt gccaaaagaa cacccggatt tacaggtgcg gatttgaaaa
     5401 atttgttaaa cgaagcagca cttcttgcag caaggcacga caaagtaaaa attcaaatgg
     5461 atgacctgga agaggcaatc gacaaagtta tggcggggcc tgaaaagaaa agcaaaatta
     5521 tctccgatga agaaaaagaa aatacggctt atcatgaagt gggacacgcg cttttgtcta
     5581 aacttctcaa agactgcgat ccgcttcaca aagtttctat cattccgcgc ggtatggcac
     5641 ttggcataac aatgactttg cctgaaaaag accacctgac cttgaagaaa aatcagctta
     5701 tcgacagaat tacaatgaca ttaggcggaa gggttgcaga agaattaatc tatggcaaag
     5761 attttataac cacaggagct tcaaacgatt tggaaaaagt aactgcactt gcaagaaaaa
     5821 tggttacatc ctacggtatg agcgataaaa tgggttatat ggcatacggc aaaagcgaag
     5881 aacacgtctt tatgggcaga gatttcggcc acacaagaga tttctcggaa gaaatcgcag
     5941 cagaaatcga taaagaagtt aaaagaatag ttgacgattg ttataacaac gccaaaaagc
     6001 ttttggaaga aaaccgcgac atgctcgaat acatagccaa aacccttctt gaagaagaaa
     6061 cccttgacga aaaagagttt gtagaattaa tggacaaagt caaaagcgaa cggggataag
     6121 tgaacggtga gcagtgaaca gagcgtgata cggcaaatta attttcaagg attaaaaata
     6181 ggcggtgagg gggtaaatgt tacagatatt tatttctcaa cttcgcatgt tgtagaagca
     6241 gggaaaaatg atactgcttg tccttatttt gctttggaat taaacatttt tgattttgac
     6301 gcatcttccc acattgttaa agattattat ggcgaaaaaa agccgattaa acttgtacaa
     6361 aaagctcaag aatcaaattg tgatattctt gccctaaaat tcaatatttc cgaagaaaat
     6421 gttgatgaaa atattttaag ggcaaaaact ctgttggcag agcttttgcc ccatataaaa
     6481 aaacctttga ttatcagagg aatgaataac aaatccgttg atgtaaaact tttgcctgca
     6541 ctgatggagg tattagacag agaggttatt gttgcctttg cagacgaaaa tacttataaa
     6601 gagtttgtgc cgcctgttgt aaaaggaggt catattttgg tgataagaac cccgattgac
     6661 attaaccttg ccaaagagat gaatatatta acgaccgata tgggtttaag tcctgatagg
     6721 attttgattg acactgatat ggggggttta ggctacgggc ttgaatacgg ttacagtatt
     6781 atggaaagaa ttaagcttgc ggcatttgac ggggatgaaa tgctaaatat gcccctaatc
     6841 gcttttgcag gcgaagaggc gcttaaggca aaagaggcaa aatcggacac tttttccaag
     6901 agtttcgggg attttaagac gcgctcttta atgtatgaaa taacgaccgc ctctgccgta
     6961 atcgcagcag gcgcaaacat tgtggtactg gagcatccca aaagcgtgga aatattaaat
     7021 aaattattac gtcatgctga atttatttga cttcttttca aggcgaggaa cgagccgtag
     7081 aaaatgtcct tacggcttgc taaaataatc cactccatga tagaattcac aatagtatca
     7141 gcatcttgaa gtcttgctaa cttataacaa tttgtgataa gtttagagaa tgaaaaactc
     7201 ttcacctatt cacctgttca actattcaac taaggaatta tatgactgaa ttgtcgggat
     7261 tacaaatttt taaatatttg cctgctgcaa aaaaattgtc cgagggaaac tgcaaaaaat
     7321 gcggtcttcc aacctgcatg gcttttgcgc ttaaacttgc aaaaggtcag gcgcaaatta
     7381 gtctgtgtcc ttatgtcagc aaagacctta aagagatgtt tgaactgggg atgaaaaagc
     7441 cccaaaaaga aatactttta gggcacaaaa acaaggtttt agtcggcgga gaaaatgttt
     7501 tgtatcgcca tgacaaaaca tttgtgaata aaaccgctgt tgctgttgtt attgacacaa
     7561 aagaaaaaga ctggcagaat aagctccaaa gggttttaga ttttgaaatt acaagagtaa
     7621 acgagacatt taaggttgat ttgatttatt tgaagggcaa atacccccct cctcaatcct
     7681 ccccctcaag ggggggggaa gatatgtctt tctcaaaact aacagacaag cttgatgact
     7741 tgggttttgc atggattgtc gaagaagact tgaaggattt aataaaggtt gaagataaag
     7801 gagtttttga aacggttgaa aaacttacca aaatccgcaa aaaagccgtt ttggaaaaag
     7861 atgacaagta ttcaactcct gtttacgttt attttccctc gccccttggg ggagagggtg
     7921 ggcaaagccc gggtgagggg ttgcttgaat tgatgtcaaa agcaagtttt tatctttgca
     7981 aatacgccaa tatgcttatt tttgaagact ttgacgaggc actgttttct actttaatga
     8041 cgctcaggca aaatattttt actgatcctc aaaaaccgct tcaggtggag tcaaaagttt
     8101 atgaattcaa taatcccgac aaaaacgctc taatattttt aacaacaaac tttgcgctta
     8161 cttactttgc gatagcaaac gaaattgaag cattaaataa acccgcttac cttgttgtaa
     8221 caccttctga ggggatgagc gttttaacgg catggtctgc cgaaaaattc accgcgcaaa
     8281 tggttgcaaa aacggttgca aagtatgagc ttgccaaaaa agttaaaaac aaaaaaataa
     8341 taatccccgg gcttttggct catatggcgc aggaattaag ggaagagtta aaaggttttg
     8401 aaataatcgt aggaccgaat gaggcatacc ttttggctga ttttgtaaaa agtattaatt
     8461 aaagcctgat ttttctatat atttaccgtt acggctgtca taacttttta ttttgtagcc
     8521 gtttttagaa ttttctactt tcagggaatt ttgtttttcg gttataaaaa ctttaacgtt
     8581 ttgactttgc agaacatcaa gggttacacc gctggggtga ccgtaatggt taggtcctgt
     8641 cgaaatcaaa gcggcttcgg gtttaagttt ttcaatcatt tttttgtcca caacgtttct
     8701 ggcaccgtgg tgtcctacct ttaggacttc aatgtctggc atgtatgggg cgattttgcc
     8761 aaacgcttca acgccggcat cacccataaa aagttcgtca aaatcgcccc ttgaaacaag
     8821 ggttatgaca gaattttcat tgtcgctgtt tttgattccc gagatgaaag tttttatctt
     8881 aaaatcgggc tcaaaataga ttatttcatt gttttttacg gcaaaagttt taactttttt
     8941 gttttttgca aggttataaa tcttttgcgc taagggcgat ttgtcatcta aagaatttaa
     9001 ataaactgca tttaccccaa ggtttttgat taaatcaacg gcaccgcctg catgatcaga
     9061 atcaaagtgt gtcaagataa gtccttcaat atttttaatt cccttatctt taaggtaggc
     9121 aaggatgata aaatccgcct gagattttcc gcctttgaat ccctgttttc cgctatctat
     9181 aataaagtat ttatttttcg gtgttttaat caaaaaagca tctgcgtttt gaacgtcaaa
     9241 aactattgtt tcaaaatctt tatcgggaag cgggatataa agtataaata aaacagccaa
     9301 agaaataatg gttgcggtta taatccgttt ttctctgccg aatttaaaaa gaagggttat
     9361 taaaacaaca agaaaataat acaaaatcat ctgaaaatca ttcggctgag gcgttataac
     9421 aagtgaatga ggcagatttg aaaagtattg agaaattgca acaagggcgt ttaaaacagg
     9481 atttagggca gagtcaaaca caaaacaggt tatatccgca acaggcttaa acattgcaat
     9541 aacagagctt atgaacccgc cgaaactcac gattgataca aaaggcataa tggcgatatt
     9601 tgcaaaaacc gaataaaggc tgaaagaatt aaaataatac atctgcaaag gcgcaatcca
     9661 gatttgagcc acaataggga tgaaaacagc acccgaaagc cagttcggaa cacgtttcag
     9721 tctttcaaag acaacaggtg cggtggtcaa aagcccgaaa gttaccaaaa atgaaagctg
     9781 aaagcccaca tcatttatat aggctgggtt gtaaattaac attaaaagtg ccacaaaaga
     9841 caaaagcgaa agactgtgag catctcggtc gattaattta ccaagcaaaa caaaaatcag
     9901 cataatcgca gctcgtatta cagaaggtcc tagccccgtc ataagcgcgt agaagattat
     9961 aacaaaaatt ccgcttgaaa ccgttatgtt aaaaggaact tttagtttgc gcaggatgaa
    10021 aaaccatatt ccgtagatta tggcaacgtt cattcccgat gcagcaagaa tatggagtaa
    10081 gcccgagttt ataaatgctt ttttgatgta atcgggtggt gccacagcat cgtcgccgaa
    10141 gactattccg cctaatattt ctaagttagg gctttttaaa tatttggcat gggtagtcag
    10201 gatttgattt ctgatgttgt taagcccttg caaaaaagcc cattttgggc taagttttgt
    10261 gtctagtttt ttaaaatcgg catcttgtgc gtaaaacagg gtaaatactc cgaagttttt
    10321 caaatatttg ccgtagtcaa actgcgaagg gtttgatgcc ttaaacggcg gtcttaattt
    10381 acctttaatg atgtaagtgt cgccgatatt caatgtcatc ccgcatttat tgcgggatct
    10441 ttcaaaacaa ggaatcatat ttatataagt ttttgccttg atttcaactt cttttttgtc
    10501 gtaagagatt tttttaacat tgaaaaagaa tttttccctg ccgttgattc cggacggaat
    10561 ggaggtgatt tgtcctgtaa taaccgcatc ttttggcgct atctgataaa gcgtgtcata
    10621 gttttttatt cttaaatttg cattaaaaaa agcaaaaaag aacacaaaat accaaaaaag
    10681 cgcaagcttt ggcgaaatat aatttttcag gattgcaaaa atcgcaataa ttaaaagcac
    10741 aaaagaaaat tcaatacttt tttgactaaa gtatgcaaaa agtcctaaaa tgtagattac
    10801 cgaagtaaaa aacatcagga aatacttttt ctgcgcagct gaattgatat aatctctaat
    10861 catttatcta atatatcaat aaaatttttt atagtaaaat agggtaataa atataaatta
    10921 aaaaatatta atataaatgt catgctgaac ttggttcagc atcttaccag cttagtctgt
    10981 tggatagatc ccgaaacgag ttcgggatga cagaaggcga gaggacagat gaagttatat
    11041 aacacatact cccaaaaaac agaagaattt atctctattg aggcaaacaa agtaaaaatg
    11101 tatgtttgcg gaccgaccgt ttatgatttg gcgcatctgg gacacgctcg ctgttatata
    11161 acatgggatg ttctgtacag atatttaaag tttaaaggtt atgaggttac atactgcaga
    11221 aacgtaaccg acgttgacga caaaatctta aacaaagccc aaaaagaggg caaaactcca
    11281 gatgaaattt cacaacacta ttacaaatta ttttctgaaa gcatgaaaaa attaaatacc
    11341 ttgccccctg atattgagcc ttttgccaca aaaactttgg gcgaaatgat tgccatgaac
    11401 aaacaattaa tagaaaaagg ttttgcctat gaatcaaacg gggatgtgta tttcagggtt
    11461 aaaaaattcg ataaatacgg tgctctttca aaacagccgc ttgacgcact tgaagcgggt
    11521 gctagggttg aaacctccga gataaaagaa aatcctatgg attttgccct gtggaaaaaa
    11581 gacgaaaaat tcggctacaa aagcccttgg ggaacaggca gaccgggctg gcacattgag
    11641 tgttcggcga tgagcagaaa atatttgggc gaaacgatag atatccacgc aggcggcgcg
    11701 gatttgattt ttcctcacca cgaaaatgaa atcgcccaat cggagtgcgc taacggctgt
    11761 cagtttgtaa aatactggct tcataacggg tttgtgacca taaataaaga aaaaatgtca
    11821 aaatctttag ggaatttttt aactatcgac ggaattttgg aaaaatacga cgcaaacacc
    11881 ataagatttt ttatcctgac caatcattac cgcatgccgg tggaattctc cgacgaagca
    11941 cttgaatcgg cacaagccgg ggtaaaaaga ctttataatg ctataaatca gtcacgtaaa
    12001 acagtaaaaa ttgacgaaaa tcttaatatg gaagcttttg atgaatacaa gcaatttgtt
    12061 gaggctatgg atgaagattt aaacacttca aaagcacttg cggttttgtt cgatttggca
    12121 aataaattaa acaaagtgcc aaacccgcag aatttggcac ttctaatcaa cttatccgaa
    12181 gctttgggct ttaattttga taaagttgag atttctcaag atgaattaaa agaaaaaata
    12241 aatcttattt caagtaattt gggaaaaact tttgctgata tggaagaaat tttacaatac
    12301 agaaaatctg ccagagaaga aaaaaactgg gatttggcgg ataaaataag acttgctttt
    12361 gacgaggcgg gcatagtgtt aaaagacagc aaagacggca caacatggga actgaaataa
    12421 ggcataaaac acccaaaaac caacgtcatg ctgaacttgt ttcagcatct tataaacgtt
    12481 atgctttggc atttttgttg tttattattt tctctttcgg tctaaaagcc tttgcgggcg
    12541 gagttttttc cattaatacc gatttaaaag tgccgtttgt atcatcagaa aaaaagaaca
    12601 cggaaaaatc ggaaatttta gaaacaccgg gagtcccaca aaaagctgca acccccacac
    12661 ctcccacgac ttccacaccc cctgcgcagc cagttgaaaa aacttctccg ccacccgaaa
    12721 ccgctgcaaa aaccgaacaa aacaaaataa ttttgccccc gactcctgcc atacagcagt
    12781 ctttgagcct caatgataat gcgcttgtaa gagttgcatt gacaaacaac aatttcagct
    12841 cgtatgcttg gggcgaagcc tcaattcttg ccacaagcga tttttcgctg attgaaaagg
    12901 aatcgggcaa ggaaattgca aagtttttac ctgcagatgt tgttaaaata aagatgaatt
    12961 caggggtttt agaggtaaaa ttaaacgaca aacccgttgt aaaaaccgct tcggctttaa
    13021 tttttacctg cccgaaaggt tttttgggaa ttgagggttt aaaaagaaat tcaaggcagg
    13081 cgatttatcg gggaaacttt gaaataaccc ccaaaagtcc gacacaattt tatattataa
    13141 acgttgtcga tgtcgaaagt tatctcaaag gcgttgtgcc aaatgagatg cctgtgcgtt
    13201 tcggtctgga agcactcaag gcgcaggcgg ttgccgcaag aaattatgtc ctgactccac
    13261 gaacgcgctc ttgcaaagaa tttgacgtgg acgattctgt tgccagtcag gtttatttcg
    13321 gggcagggac agaggattta ctctccgagc gagctgttga cgagaccaga gggcttgtgg
    13381 cactatacgg ctgggattta atcgtggcac aatatagctc cacagcaggc ggctacaccg
    13441 aaagctacga gaatgttttt tcagatccca agacaaaaga atttccttca aagtcaagac
    13501 cttatttggt cggaactccc gatattatag gtgttttgcc gcttgacagc gaagaggctg
    13561 caagaaaatt ttatatgact gcgccggatt cttacgatat gaaatcgcct tactaccgct
    13621 ggcaaaaagt ttgggaagga acagatgagt taaaaaatgt tttgcagcaa acccttgtgg
    13681 tgcagtcaaa aacagggttt gttaagccgg aatttaaaaa aggtgacatt ttaggcgaaa
    13741 ttaaagaact taaagtcaac aaaagaggcg tttcgggcaa agcaatggag cttgaaatag
    13801 taactgacaa gcaaaattac aaaatttaca aagagcttgt aataagaagg cttttgacca
    13861 aagaaggcaa agcacttcca agcgcaaatg tggtttttga ccacatttgg gatgagaatg
    13921 gaaaactcac caaagtaaca gcctacggcg ggggctacgg acacggggtc gggatgagcc
    13981 aattcggagc aggctttatg gcaacttcgc tgaacaaaag ttttgacaag attttaaaaa
    14041 gatactatca gggaatcact attgccacag tgcctctgat tctttctgac agccaaaatc
    14101 aacatgtcgg aatacaaaaa ttttatgcgc ctaagcaaag tgctacggtt gttgttgaca
    14161 acaagtacca aataaaacaa tttagtgcta aaattaactc aagagacgta acttttgagc
    14221 ttgcaaacag ttttattcct attcaccggt acagcagaat tgatatttca agctacataa
    14281 gacgcggaaa aaacgaaatt attttctact tcccgcaaaa ttgtgaaaac aaagccataa
    14341 gagtttacgt cgagctggtt gaaaaaagtg aaggttacga attttgagta cccaagaaaa
    14401 tgtaagcgcg gaaaatgaaa aaggaccgag tctgctgaaa gcggcatggc ttattgctat
    14461 ggtcactgtt gtgagcaaac ttatcggctt tataagagat gtcgtaatcg caaatttcta
    14521 cggggcatca atggtttcag atgcttactt ttatgcctat cagattccag cccttgcaat
    14581 aattctgcta ggcggagtcg ggggaccttt ccacagcgcg actgtggcgg ttttttcaaa
    14641 gcttatacct tcttttaagg aaaaagcaaa cgaacagtta aataaacttt ttaacacctt
    14701 tttgaccgca agttttttgt tttttatggt gttggcggtt ttgtgttttg ttttttcaga
    14761 ccagataatg gggtttataa tctcgggtac taaccctgaa ttaaccgcac ttgcaggcat
    14821 gcatcttaaa attatgtcgc ctgttttgat cgtgggcgga attgtgggaa tttattacgg
    14881 aattttgata agttaccgcg aatttatcct gcctaatgtt tcgccgattt tgatgagcgt
    14941 ggtgattatt ttgatgattt ctgccgtaaa aggcgacaat actgggagtg tgcttgcttg
    15001 ggcaactaca ataggtgctc tgtgccagtt tgtttatcag cttcctaaaa taaaacagtt
    15061 aggttacaga ttaagaccga atttcgattt ttataacccg cagtttaaaa atatctgcga
    15121 acttttgttt cccgcaattt taagctcaac tgtgggacaa attcatattt atgttgacat
    15181 gttctttgcc tcttctttaa aagaaggcgc atggacagct atcggctatg caaacagagt
    15241 tttccagttt cctgtgggga ttttggtaac agcgttttta gtcccgcttt tcccgatttt
    15301 ctcaagactg gtggcacagg aagattttga gggaattaaa agctacttta acaaaggtgt
    15361 cggggtactt ctttttgccg cagttcctat aataataggt attctgacgc tcggatatga
    15421 tggaatcagg cttattttcc aaagaggagc atttgatgat aatgcgacgt ttatggtaac
    15481 gcaagcttta tggtttttgt ctttttcgat tctgccctac gttttcagag attccataac
    15541 aagggtttac tattctttta atgactctgc cacaccgttt ttggtagcgt tcagctcgat
    15601 tattttgaaa ttctttttaa actggctttt ggttgcaaag ctttcaatcg gaggcggaat
    15661 aggcggaatt acactttcaa caacacttgt aacgcttttt aacgcggttg ttttgggtgt
    15721 tttgataggc agaaaaatca aacttgatta taaaagtttg ttctcaaatt ttgcaaaaat
    15781 gtttacggca ggggcggtaa cactttttgt ctgctgggca atgtgtatct ggtttgacac
    15841 attgatgctt cctcgcacaa tctttgaaat ttcaaaaatt ctttttgtga cttcaatctg
    15901 tcttgtttgt tatatttatt tgaatttgtt gtataaaatg gactacgcaa tcgagctcaa
    15961 agaaagattt atcggaaaaa tgagtgaaaa atatggctta tttaagtaat caattaaagc
    16021 aaaaaataaa aaacacttta gataatgacg gggtgattgc ctttgttaca gacaccgtat
    16081 ggggcttggg ctgtttgcct tcgagtaaaa aagcggttaa aaaaatttat gaaattaaaa
    16141 aaagagaggc taaaaagcct ttgattttaa tgtcaaacag tgttgaaaat cttttgcttt
    16201 acgtaaaatc gttgagcaaa aaagcaaatg aacttgccga aaaatatttt cccggtgcgt
    16261 tgactcttgt attaaaaaaa tcaggtaaaa ctcctgattt tataacttca ggatttgata
    16321 ccgtcggcat aaggattcca gacaacaaag tttttcaaga aatctgtgaa tgtattgaag
    16381 gaaaagttct tgcaactaca agcgcaaatt tgtccagcga accttcggca aaaacttata
    16441 aacaggcgct tgagaaagtc ggaaacactg cagatttagt tatagaagac tttgggcaac
    16501 ttgcaaaagg tttagagtca acagtggctt taatattaga aaacgaagtt cgagttttgc
    16561 gtcaaggtgc agtaaaattc gacatttgat atgattttaa atgtaaagaa ttgtttaata
    16621 tttaagcctt actagcaaaa taaattattc agatgattta tacattggtg cggttgtttg
    16681 ttgtatcaaa taattgtata atatttaaat aaacacaagg gcaagagaaa tggtagataa
    16741 tagagcagcg ggtttctttg gaataggcgg cgcttttaat tttaaaagcg gcgacatttc
    16801 aggtaccgcc gcaaaaactc aagacgataa aatgggtccg gggacagagt actacgccca
    16861 aggacaaaac gaagaagaac aaaatcaata tgaattccct tttattgaca gaagcgcaca
    16921 gcttagagcc acgcttaatt ctcttgcaat gatgaatgtc gccaacgtat taaattcaaa
    16981 aaaacgaacg actttccttg aagatatgga agagttggaa gaagaaaaag agtacgaaga
    17041 aaatgaagaa tttatggacg attttgacga agataattat gaagaagacg ggcaggaata
    17101 ttattagctg aaaagctgaa tgggtgaaca gctgaaaggt taatattttt gttgcttagc
    17161 aacaaattga acttattcac ttatttacca tttcctctct tgcaaaaaaa ccttgtcgtg
    17221 ctaaaatcag acagagtttg tttataagga gagaaatatg ggttgcaaat tagcggataa
    17281 aaatttgacc tctaaagttg tgcaagaagc acttaaagcc ttagacaaga aaaactttgc
    17341 tctgatagcc cattcaagca gtttcccttc tttgtccgat gaagatactg ggtttggcag
    17401 tataaattct gccgcaggca aaaatttgat tgattttgca tccggagttt ttaatgcaat
    17461 tcaactgggg cctgcgggta aaaccaaagc ctgtgattct tcgccatata ccggcacaat
    17521 tttttccgga aacccgcttt ttattgattt aaaacaacta actacaaaac aatggaacaa
    17581 tattttatca gaggaaactt ttaattctgt tgtgcaaaac aatcccaaca agaattcggg
    17641 aaaaaccgct tattcttata tttataaagc ccagcaaagc gcgttaaaag aagcttatga
    17701 gaattttaaa aattctgctt ctgaaaaaat aaaagaagaa tttgaaaaat acaaaagaga
    17761 aaacaacaca tggcttgaaa aagatgcact ttatgaagca ctttcgattg aaaattgcaa
    17821 cgattattgg ccgctttggc aatctgaaac tgataaaaat ttacttaacc caaagtcaat
    17881 cgaagaaaaa atggaatttg caaacaggat aaacgagatt gaagcaaatt acgaagacga
    17941 aataaatttt tataaattct gccaatttgt tttgagtgtt caaaacagtc aaacaaagga
    18001 atatgcgctt tctcagggca taaaactaat cgcagaccgt caggtggcat tttctgacag
    18061 ggacacttgg gcatatcagt ctttgttcat ggaaggctgg tttttgggct gtccgcccga
    18121 ttatttttct aaagatggac aaagctgggg ctttcccgta attgctcccg aaaaactttt
    18181 taagcccgac ggttcgcttg atgagggcgg agttttaatg aaagaacttt ttaaaaaaat
    18241 gttcaaagaa aaccccggcg gagtaagaat cgaccacatc gtggggctta tagacccctg
    18301 ggtttacaaa gcgggcaaaa atcccaaagt agaagaaggt gcgggaagac tttattcatc
    18361 gcccgaacac cccgaattgg gcaaatatgc aattccatcg cttaacgact tgaaccacga
    18421 ccttgaacca gataaggaaa aaagagttca aacgctctct gacgagcaga ttaagcttta
    18481 cggaagaata atagagcaca ttgtaatagc agctgctaaa gaagaagggc ttgataagga
    18541 ttctattgtc tgcgaagatt tgggaacttt aacaaatccg gtcgctgcgg ttatggaaaa
    18601 atataagctt caggggatga aattaaccca gtttgtatct ccggaaatgc aagatcatcc
    18661 ttaccgctgc cgcaacatag acaaaaattc atgggcaatg gttggaactc acgataacga
    18721 gccgatttcg atgtgggcgg aaaatatgat taacacacac gaaggttact tgcatgcaaa
    18781 aaatcttgtc gaagatttgt tccctgatga ggaaaataaa gatgatataa taatgaatct
    18841 tacaaaagac ttgacgtttt tggcgcagac caaattggtt gagattttcg caagccctgc
    18901 ccaaaatatt cagatatttt ttacggatta ttttgggata aaagacgttt acaaccgtcc
    18961 gggaacttca ggagacggaa attggaattt gagaattccc aataattttg aagaagtttt
    19021 ctgcaaaaat ctgaaagagc aaaaagcact caatcttgcc ttaactctga aaaaagcaat
    19081 cgaggcaaga ggaaacgggt ttgtcgaaaa acataaaaag ttattgggtg agttaaacaa
    19141 attaatttaa tcggggaaaa atgaaaaaaa tattggtttt acttctgata ttaatttcaa
    19201 cttcgctttt tgcaacagcg caagaagcca ataccgaagc tgttttgcag tataatcaag
    19261 gaatcgatta ttacaaggca ggcgaatacg acaaatccac taccgcattt aaaaccgcaa
    19321 taagccttga ccctgattac attgacgctt actataatct gggctctgtg cttgaatttt
    19381 tgggcaaata cgaagaagca cttgtcattt ttaagcaggt ggtggtaaga aagcccgacg
    19441 attacgattc tgtttataaa gccgcatggc tgagcaaaca gctcgggcaa aaccaaaaag
    19501 ctctgactta tttgagtata attccaattg actgcgagcg ggcaaaagac gctcaggtac
    19561 ttgcgcaaga aattaagcag cagggcacgg ttcaaaatat cgatgtttcg gcaccgatta
    19621 aggaggtaaa aatctccgag cctaaaatca gccaatcaaa ttttgtttat caaaatattt
    19681 tagcaccaac ggggataact tctgattctg aaggaaacct ttatgtggcg gagtttaaca
    19741 acaatttgat aacaaaaata acgcccgata ataaaaaaat aatttttgta aaagattcca
    19801 aaatcagcgg tccgataggg cttgcgactg ataaaaccgg aaacatttat gttgcaaatt
    19861 ataacaaaga caatgtttta aaaatttccg ttttcggcga aataaccgtt cttatcggta
    19921 acctcaaaaa gccttattac ctttatgtaa aagacaatat gctttttgta tcatgtcagg
    19981 gcacagacac ggtgctgaga tataaattga attaggctga atgggtaatg ggtgaggagt
    20041 gaatgggtgc acctcatccg tattcacgtc attgcgaggc tttgaaaaag ccgtggcaat
    20101 ctatccgatt gagattgttt tcatagaatt atttcttaat gctcaaatgg aaagattgct
    20161 tcgtcagaaa taaacttctt cctcgcaacg acgtaatttt ttgtaattgt tcatctattc
    20221 acttctattt actatttacc atttactatt tcctaactta tccccaaatt gtccaaaatc
    20281 tcgcttgggg ctttggatga gttcagtgtg taaaagtgaa gtccgcaaac catattggtc
    20341 tacatgtaac aaaatactta ataagtccaa aaataaagca aattccgatt ttttaaaacc
    20401 ttattaataa tataaggtaa attcaggtgt cacagtggta gattctatta ataatgtgaa
    20461 tagtagtgta atcggacaaa attataaaaa gccgagtttt tggaattatg ccggtggtgt
    20521 tgctgctgga agtttgattt caaggcttcc gtcagtttta ttaaatcaag tttctatgtt
    20581 gtcagaaaaa aaatataaaa aattgataca attaaatgca gatgagttta aaaacgtaca
    20641 agaggcggtt aataaaactt tctcttcttc aagattgtct gaaaaagggg tatcaatatt
    20701 agaagtaaca cagaaaaata ttaaagaagt tacagaagct attaaagcaa ctattgataa
    20761 aaatatgagg tttcgttcta aagaatttag agaaatgtgg accaaaatag aagtagaaac
    20821 tacaatgagt ggagatgcgg cgcaatattt aacggagtct aaaaaaatct tgttcgataa
    20881 aaaatcactt atgtctgctt ttcatgaaat tggtcatgct gtaaatggaa atttaagtaa
    20941 aatgggcaaa attatgcaaa aatgcagatc tttaagtttt ttagctgtgc ctatagcttt
    21001 gattgcttta ttgaaaaaca aaaaatcacc tgatgaaaag ccaaaaaata aaattgataa
    21061 atttactgat tttattaaac ggaatgcagg aaaactaacc tttatatcgg ctttacctat
    21121 gttggcagag gaggccttgg cttcaataag aggtaatagc tttgccaaaa aggctctgag
    21181 tcctgatctt gccaaaaaag ttgcaaaggc taattttgca ggattttgtt cttatcttgg
    21241 acttgttgtt gcaagttcta ttggcatatt tcttggaata aaagtcagag atgccattgt
    21301 aaaaccaaaa aatgttgagg ataaaaaata attatcccct caaattgtcc aaaatctcgc
    21361 ttggggcttt ggatgagttt agtgtgtaaa aatgaagtcc gcaaatgccg aattttaaaa
    21421 gctcttggca ttgttgtgtt gcaaattcca cacccaattt tttaatatca tcggggttat
    21481 ctttaaattt ttcaagcttt atcctaagtt tttcaggcag gctcactctt gccatagaaa
    21541 gcattttttc aagctgaccg tagcttctta tgggtaaaat ccccggaatt atcgggactt
    21601 caatacctaa atctcttgcg ctttggcaaa aagaataaaa cttttcgttg tcaaaaaaca
    21661 tctgcgtaaa aacagcatcg gcccctgcct caacctttct ttttaaattt ttcatatcaa
    21721 gcatgaaatt atcacactcc aaatgccctt cgggatagcc cgcaaccccg attgaaaggt
    21781 ttgaatgggt ttttataaat tctacaagtt cgtttgcata gtgaaagtca aaatttttca
    21841 ccgaacaatc gcatggcaca tcgcctctta atgccaaaat gttttttatc cccaaggact
    21901 ctatctggct taaatattct aaaatatgat ttttcctcac acaaacacag gtaaaatgcg
    21961 gcattacgtt aagtccgact tcactttgga tttttttaat catatcaaaa gaatttgttt
    22021 gagtgcttcc tccggcaccg taggtaagcg aaacaaacgc aggattatat ttttttaaag
    22081 tttttaaatg cccgagtaat ttttgaaatt tttctttagt ttcatcatct tttggcggaa
    22141 aaatttcaaa tgaaataacg ggttttttgc tgtttgaata atgttctttt aaagtcatgg
    22201 gaaaatttta gcataagact tatttaaccg caattatgca gtcgtaaatt ttgtcgattt
    22261 tttctttcat gtccgaaaac tgttctttca aatccttaac gctgtttagc gtggcaaaac
    22321 gttcttcggc atcttttaaa atttctctgt gctttctttc aagttgttcg ggcgttacga
    22381 caattctttg ttgtatcaaa aaaaccataa ttgcaattat tatgggtgaa tagtgtaata
    22441 aattttccat tttttctccg tttaaatgct gataggccag taaaattcga caagataaga
    22501 tgccgcgtcc atagggtgtc ccagaaattt taattcgctt gaatttttaa tctgctgata
    22561 tgaaggcagg tcaatctttg aagagccttc cttgtacctc aaattataaa tgttataaag
    22621 aagtttttca cattttttat ctgtaaaaag ccttatttta ccgtctgcac cgtggacttt
    22681 ggcgttaaat gccgcaattc tgtttttaat cggcgggtta aatgccttaa ttcttatttc
    22741 cacatcttca tagccgaatt gtaaaagctt ctttttcata attacatagt tggtaaattc
    22801 gcttgtacag cttctgttat cgcctgaggc atcgccgttt atgataattc tgcctttgtg
    22861 attgggatat ctgtggtaaa actcgtcgca agcttttgca gttgtggtgt cttctatgac
    22921 gatttcatca aaaaagaaaa ctttgtcttg cgttttgtgc gctaaaatcc acgccatagg
    22981 atctacgtta aagtcgcagg tgatgtgcaa atccatatcc tgctggtatt ttatttcttt
    23041 tatgttttca tcggtgaaat cttttattat aagccctttg tcatattcgc cgttttgcgc
    23101 cagtacaaaa attctgtaat actgttcgtc gtagagtttt ttgagttcct cgcaaaagcc
    23161 ttccggcaga taaatattat ctgttgtagg ggctgaaatt aatctgtaat tgggtttgtg
    23221 ctcatcaaca aaggttttgt aaatccagcc tctttgaact tcagggtttg tgtggccgaa
    23281 aattctgtaa gtaaaatttt tccagccctg cttgattttt tgccgcatcc ttcccaataa
    23341 catcttaaaa gtggaaaaag gaatatccga catttcctcg atttcagcaa atcctaagtt
    23401 gagcgatttt aacttatttg gctcgtcaaa atgtctgaat aaaatctccg aaccgttttt
    23461 aaacgttaat ttctgcagtg aactcgacca gaaataatct tttccgtctt tgaagtttaa
    23521 attctccaga tgctcaaagt aagactgcaa ggtggtgtcc ctcacaagcg tgtaagtctg
    23581 agccccgacc agtcccctta ttccggggaa cttcaggcac aatagtattc caagaagcga
    23641 gccggcaaaa gtcttgcccg agccgaagcc tccctgatac acagctacgt ctaaactgta
    23701 atcgtgcgga atttccaaaa attcccgctg tgctgttaat aacttgtatt tcattttctc
    23761 cttatcttaa aaagtaaata gtaaatagga aatagtaaat ggtgaataga tttttgcctc
    23821 tatttaccat ttactattta ccatttacta aattattatt ctaattgccg ttgctttcaa
    23881 ctagaaaatt attcgcgttt tctattccgt actgttcgag agcaaattta aagcactcaa
    23941 tccagttaat ttgatttgtc atggtaggaa cttctgcaaa tgctcttatc acgtcaaaga
    24001 gttcttttga gcgaagtttg cgctccaatg tagcttttct gtcgccataa cggtagatgt
    24061 atttggcgtt tcttattgtg tcgtcaattt ctataaaaag ggttttaccc tgatcaaaaa
    24121 cgcttattac ctctcttccg attttaaaat tagcgattaa ctctgctgtt ttttcaacca
    24181 tggggataat aattttcctg tttatggcat caagcatcat attaagcctt gttgtctgtc
    24241 cgcttacgga ataattaagt tcggttgcgg ttctttgagc ggtttgaata tttcctgcca
    24301 tgtttttaaa aattcccgtt gcactttcga ttgttgtttt aaaataattt aagaaatccc
    24361 atccgtgaag ggctttttca aaacttaaag gtgaaggggc tgtcggcatt aaagaagaat
    24421 cgtattcaat gattttgccc ggttttacca cctgttcgcc tttaaaacag ccttttggcg
    24481 caagatacgg cgggttcatc ataagggcaa gggcatccac ctgtttattt aaaattgtgg
    24541 atgaaatgtt atttaaaatc agagccaccc tcaaaggtga aagccctctt cctgttgagg
    24601 ggtttt
//
LOCUS       DAOQAU010000023        23142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2224_length_23142_cov_6.462685, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000023 DAOQAU010000000
VERSION     DAOQAU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 23142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23142
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_23142_cov_6.462685"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1526
                     /locus_tag="PKI94_04860"
     CDS             117..1526
                     /locus_tag="PKI94_04860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26104.1"
                     /translation="MNPKISIIVPVYNNEKFLSECLDSICRQTFSDIEIICINDGSTD
                     NSLSILEEYALKDKRIILLNQENKGLGYTMNRGIKTASGDFIGIVESDDYILENMYES
                     LYKIAVKNDADFVKSNFCVFYDEENSRKFIQVPLFDDEKYYNIVLNPKKSPNVFNAIM
                     NNWTGIYKKDFLEKNKIYHLETPGASFQDTGFWFKAFCCAERIFFLDKEFYMCRRDNP
                     DSSVNSKEKVFCICEEYSEIENFLNSRPDLKKYFEKIKNHKKFQMYQWNFTRMGSKYK
                     QMFIKNASKEFKLAQKKGMLDKNLFGAGEWKKVQFLIKHPVLFPLKYNLLSPYTKKRK
                     ELKIHFKKYFDCVRAQKQINKLARKYKNKKIVLYGAGIYCKTLFENYDLSNLNIVAVA
                     DRGFKNEENKNFFNLNCILPKDLKEFDCDLILLGVYDTLQIFDYLQDQLLVGSKNSQI
                     KILPIVEDTVISRIKNFFS"
     gene            complement(1507..2862)
                     /locus_tag="PKI94_04865"
     CDS             complement(1507..2862)
                     /locus_tag="PKI94_04865"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNW26105.1"
                     /translation="MPAIVSKTEKNSIAHELEIQSGDEILSIDEQKMSDMIDYNFLCK
                     SELVTIEIKKINGEIEVIEIEKDFDDDLGIIFESAVFDKVKPCCNKCIFCFVDQQPEG
                     LRETLYIKDDDYRLSYLQGTYITLTNLSEADKKRIEKLRLGPLYISVHTTNPELRVKM
                     LKNPKAADILKHLKWLEKIEIPIHAQIVLCPKYNDGEELKRTLFDLKKLKNVVSIAIV
                     PVGITKFQKGKLYPVTKKIAIETIKIVDEFINVPLTPALSRKGRGGITKSNLTICCSD
                     EFFSLAEANIPPRKYYGNFSQLEDGVGALRLLLDDFGKEKKRLSKSLSEPKNILFATS
                     KLAEPAIKEICTELNKVKNLKADYVAVKSDYWGENITVAGLITSEDLINSVMTNPFTL
                     HSSLLTVIIPSVMLRPYTEDFLDGKTLDYVKEKTGFNFIVINDFYSTKEIIEFIMKKS
                     S"
     gene            complement(2864..3385)
                     /locus_tag="PKI94_04870"
     CDS             complement(2864..3385)
                     /locus_tag="PKI94_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNW26106.1"
                     /translation="MIELLILYVLSEREMTMYAVLKAILDNFAAFSQPSFGAIKPALN
                     RLEKEGVIRSRKAISEGGKLSGYYSVTSEGREKLTQLLLEKLSDNPLQFASNARIKLC
                     CASYLAKDERKILFSLIKNKAFEHKHSAEKILANDYNSLNFYQRIILDNSIVEYNNLI
                     SLIESLDKENNKV"
     gene            3510..4250
                     /locus_tag="PKI94_04875"
     CDS             3510..4250
                     /locus_tag="PKI94_04875"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNW26107.1"
                     /translation="MTKTLTKQDVAEIVKKTNLQHIAIIMDGNRRWAKKKNLPSAIGH
                     KKGVDSLKTALKSCHKFGIKYLTVYAFSTENWNRKQEEVDFLMDLLAQTIKKELKDLH
                     KNSVVIKFIGDLSKLNPKLQKILFNAEEVTKDNTGVNLQIAFNYGSRDEIVNAVKNIS
                     QKVLNKEIAPDKITEEIISKELYTSNIPDPDLLIRTGGEKRISNYLLWQIAYSEILIT
                     GEFWPDFDEQSMEEAILEFNRRQRRFGG"
     gene            4254..4835
                     /gene="rdgB"
                     /locus_tag="PKI94_04880"
     CDS             4254..4835
                     /gene="rdgB"
                     /locus_tag="PKI94_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNW26108.1"
                     /translation="MFKIVLATANKHKVEEINAICEGFGIKFIMPPKGFDPIEDGKIF
                     EENALIKAKAANKLTGLPSLADDSGLCVDALKGFPGIFSARYAKTQSEKIKKILAEMK
                     NIPNENRGAKFVCAMVLLDKDGKILFQTRGECHGKIALEAKGQNGFGFDPIFIVKGGN
                     STMAELSEEEKNKISHRGIALQKVLNYTNKKLI"
     gene            4938..5438
                     /locus_tag="PKI94_04885"
     CDS             4938..5438
                     /locus_tag="PKI94_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNW26109.1"
                     /translation="MSSTDIAKVDFDKVNEIILSCGPKKSNVIAILQKIQEEFRYLPE
                     DALTYIGTKIEGLSPATVYGVATFYNQFSLDPKGKYIIRVCDGTACHVRGSMPLLTAL
                     KAKLNLKEGKFTTDDMMFTVEIVSCLGACSLSPVIVVNGKVYPKMTTDALKIVVDTLL
                     KQERGE"
     gene            5441..7276
                     /gene="nuoF"
                     /locus_tag="PKI94_04890"
     CDS             5441..7276
                     /gene="nuoF"
                     /locus_tag="PKI94_04890"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNW26110.1"
                     /translation="MTIVEVKQEEAKKNIAAQGTRILVCAGTGCVACGALEIIEEFKK
                     KGLDASTIQSDKMTVVPTGCHGFCEQGVLILIPDLGINYVKVQKEDVDEIIDSVKTGV
                     PVERLLYVDKDTGEKIQKREEINFYKKQNRTALGNVGKINAEEINDAIAFRGYEALEK
                     VLTKMTPDEVIKEVTDSGLRGRGGGGFPTGKKWFFTRVAQGDKKYVVCNADEGDPGAF
                     MDGSLMEGDPHRLLEGMAIAAYAIGADEAYIYVRAEYPLSIRRLRIAIKQAEENGFLG
                     KNILGTGFNFEIHIKEGAGAFVCGEETALIASIEGERGMPRSKPPFPANKGLFGKPTL
                     INNVETYGNVPGILLNGASWFAAFGTDNSKGTKTFSLTGDINNTGLIEVPMGITLREI
                     IYDIGGGLRGGKKFKAAQIGGPSGGCLTEEHLDMPMDYDNLTKAGAMVGSGGLVIMNE
                     DTCIVEIARFFASFTQRESCGKCVPCREGTKQMLAILEKIVAGKGEMEDLDVLEDLAI
                     AVKDGSLCGLGKTAPNPVLSTLKYFRDEYIAHIKDKKCPAGVCTAMKKIVITDKCIGC
                     TKCARTCPVAAITGNVKEQHVIDQDKCIKCEACIGVCPVKAIIKE"
     gene            7316..9088
                     /locus_tag="PKI94_04895"
     CDS             7316..9088
                     /locus_tag="PKI94_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNW26111.1"
                     /translation="MTEDKKTLFIDGKEVEFSDEKNLLEVIRKAGMNVPTFCYRPDLT
                     AFGACRMCVVEIEGRGIQTSCTMPPEAGLKIHLNTEKVRRIRKTVLELLLANHDRECT
                     TCEKSGHCELQKYSEEYGIRDIKYPTIKKEDFLPLDTSNPSIVRDPNKCILCGACVRA
                     CAEFHGDNHAILGFANRGSKTRVQPMAGRDFSKTDCILCGQCQAVCPTGALTVVSDVD
                     NVWKEINNPEKKVVAQIAPSIRVALGELFGLEPGENSIGRITAALRRIGFDLVFDTNF
                     AADLTIMEEGTEFIERLKSGKNLPIFTSCCPAWIKYVEQSRPEYIKNLSSCKSPQSMM
                     SPVLKELLPKYFEGYTKENTVIVSIMPCTAKKFENKRQELSQNGIPDTNFVLTTKELG
                     RMINEAGIDFKNLEPEKEDSPFGDYTGAATIFGASGGVAEAAARTAYELVTGKELTDV
                     NLKPMRGVNASERSKEMELDLNGLKVKVRIVSTIAEAEKALKELDNGTADFQMLEVMA
                     CPGGCVNGGGQPYSFNDTTIKAKRAAGLYKEDAELKFRKSHQNPDIKKLYAEFLENPG
                     SHKAHEMLHTHYEDKFKGSYRDLK"
     gene            complement(9162..9971)
                     /locus_tag="PKI94_04900"
     CDS             complement(9162..9971)
                     /locus_tag="PKI94_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26112.1"
                     /translation="MPVFALELDTSIDDEIRKNYNPSKIEQDMALPPLPKTLNQIDEK
                     EFQTSKPQPQQKQQAQVQANYQAKSYQQTNPQAYAVLKKGTKFRGKLINSVSDRSRKG
                     LSVKIVSRYPVSTTYFTVPSGTIFNGEMLDVHKPQFGGNGGLIVIRFNSMNIDGKTYP
                     IDAYVSKVNFKKTFFNNIKGERKYRKGIVSAAKPGCSFFGKMIRVSGNLASEGSGIIL
                     VPFSLFIGVVAFGANIIAAPAVAVFTRGGSVYIHEGSEFEIKLAQDVYIYN"
     gene            complement(10077..11444)
                     /gene="gltA"
                     /locus_tag="PKI94_04905"
     CDS             complement(10077..11444)
                     /gene="gltA"
                     /locus_tag="PKI94_04905"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNW26113.1"
                     /translation="MLERQRPHEQDKIKRRSNFEAVEENLTKEQVKLEVQRCLNCKNA
                     KCIQGCPVNINIPGFIAALKDDNLELAGEIIRRTNLLPSVCGRVCPQERQCEGNCVLG
                     IKGKPVAIGALERYVGDNTRAKAPEIKLSGKKIAIVGSGCAGIAAAADLKKEGHEVVV
                     FEALHKLGGVLRYGIPPFRLPRNFLDREINALKEMDVKFKTNVIVGKSITLKQLEEDG
                     FDAIFICSGAGLPKMLGIKGENLNGVYSANEFLTRVNLMQAHEKHTPTPLKIGDKVAV
                     IGGGNVAMDSARTAVRVGAKEVYIVYRRTEAELPARIEEIRHAKEEGVIFKLLHAPVE
                     IFGQDGHVKSMKLEVMKLGEPDESGRRKPVSTGKFVDMDFDTIIVALGTSPNPIIQKS
                     AQAEGIEIKTDKRGYIVIEEDTNKTSIAKIYAGGDVAPAGESNAINAMGAGKKAAKAI
                     NEMLK"
     gene            complement(11497..12717)
                     /locus_tag="PKI94_04910"
     CDS             complement(11497..12717)
                     /locus_tag="PKI94_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNW26114.1"
                     /translation="MKKNIVRVLIILVIGAIFLLAKKSPKENQLVFWTLQMGDFAPYM
                     NGVITEFEKQNPEIKIKWVDVPFSEGEKRTLAAILSDNPPDLINLNPDFSAILAQKGA
                     LKEINESNTGQFNSEIINSLKYKGKLYALPWYATSAITIYNRGLYKKSGVKLFPRNYR
                     EIWTIAPDIKEKTGAYVFMPTITENDTMLKILNKYGVNSAGKINSQAAVDVFDFYKEL
                     YRRNLIPKETITQTQREVLEKYMSNQIVFFQAGANFLNMIKENAPSTYKNTDVSSQFT
                     GALGQYDFSLMNLVIPLKSSKKEDALKFALFLTNDENQLKLAKLTNVLATNKKTLRNE
                     FYTKYDENDLMSKARVISARQLNRVQPVLRQGKGQKEINLLINTAVQEILLEKQDTKT
                     ILDDVAKKWKLISD"
     assembly_gap    12914..13013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13014..14321
                     /locus_tag="PKI94_04915"
     CDS             <13014..14321
                     /locus_tag="PKI94_04915"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNW26115.1"
                     /translation="YNYTMNNLFTELENQNKQIPLADLLRPKNIEEFLGQSNVISKNS
                     PLLNLLKTNRLFSIILWGTPGCGKTTLARIIALQTNSQFIEISAVTSGVKDIKEAVES
                     AKMALRNGTKTILFIDEIHRYSKTQQDALLPHIENGTIFLIGSTTENPSFQVIPALLS
                     RVQVIRLNSLDDESVKNIVIKGFDYLSQNYQKITFDEKITDFIINHARGDARVALNLV
                     ENSYFASDIADNKRILSIETLEQISQKRSTRYSRDEHYDCASAFQKSLRGSDADASIY
                     YLAKMIASGEDPRFIARRLIVTAAEDVGNADTNALAVAINAYKAVELLGFPEARIPLS
                     QAVIYVAKAPKSNTTVCAIDQALSDIDNGKDFPPPMHLRDSHYKDASKYGFGQGYVYS
                     HSAPEQYQQFLPDELVGKKYVKDEPKLNVCPKNKDYKNELGNI"
     gene            complement(14372..14791)
                     /locus_tag="PKI94_04920"
     CDS             complement(14372..14791)
                     /locus_tag="PKI94_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26116.1"
                     /translation="MVNAVSGVNFTSAKAMDKLSPEMLQSQGLYVGPDTGAAPTAQTP
                     KKKGGFGHFLWTLIKDAVIIGGLALAARKLLPAIKEVNLNKALPEDAKILEKAKVAVA
                     KAGDWVDDKVVKKAIEIVKSISKTKKNNQMEIFELIG"
     gene            14956..17136
                     /locus_tag="PKI94_04925"
     CDS             14956..17136
                     /locus_tag="PKI94_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26117.1"
                     /translation="MSILNKNIVKITSEDLKDATLSSAGFDGETQKRAFANILGARLA
                     IKLLFSKKIEANNIYSLYTMQSVLKNIDISDIYFENIKIDVRLVFDKNEIFIPKSHFE
                     YDILPDIYIPLLLKKDMSSAEILGFFEPKEIDKNNSNKDFYFFEADKLHKPESLKKFI
                     SLFIQKSPKKSKLEDLQKSESLFGEFVDDEISDEDKLFLIQQLNANLYLREKLVELEN
                     FELLSKKSISEEVFSHDMLFEEGINEEIPEEKSLEISEEESEIKSEDFLELPSEGVFE
                     EQLHEELTEQDLENIQEEVPFIEEFDSALPDEKQEPESAVQEEEIKEQNYQSELVSLD
                     SLVGEELFTEGLPEKQENIDVPLEEIDFSQFESIGEVQSDLAEKNEDLDAEAEELISK
                     KNAVSESEEDTDDESIKSKIDELLEELETSEEAKEALSDEIEIIEGLVSSSEGKSSES
                     FDLSDFETVSSDELEFFTPEAETEAEAEKKDKDLINILFEKENIKATDDMDSLSEPSV
                     HKKAYNININKKVIVAASIAGLMLVATVGGVLITKHNSQNNSIVQNEINQNIPPAATD
                     NTMPQDFTQNAYQPDGNMPLTPPPGQYQSQGRDMSKAVSDAFLSTPINATVSKIAWEA
                     PEDLAYSDSFRKYLQMAGKNLKLTLQNDLLLSSEMAYSNKVIVDISMNKGGTIKTANI
                     SVSSGSKQIDNIVLQSVKETLKYLKMPTDEIQGSSVDLTLIINF"
     gene            17197..17775
                     /locus_tag="PKI94_04930"
     CDS             17197..17775
                     /locus_tag="PKI94_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW26118.1"
                     /translation="MELAQQKVDLIKKIIKSDKKYLNNEDLFEDFFNESYKRSFLVAK
                     TVENENALETYLRKIITTSIVVVLKDSGRIQRTSEGFVPVKEEPLEVVSQPIIENKYS
                     NIQVSYDIIDLSEGPEEITIKKETLQTLVDAVKAAHKKNPSKQYLELYDLRYSRGLKQ
                     KQIAQELNLSQSEVSKRLLELMEHVKSKFNQD"
     gene            17797..20856
                     /locus_tag="PKI94_04935"
     CDS             17797..20856
                     /locus_tag="PKI94_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26119.1"
                     /translation="MICPKCKREIPENSLKCNHCGSRIASLCKKCNAYNSIYNIKCTN
                     CGEELLKVCPSCKGINLPTAQKCRKCGIDFINKTVQPAPVAMPEEKSQLEFQEAKPEV
                     SQEIPQEIKTHKVEEVTEKIQYSANFQSQQNAKDSLVKALISGNSKIISLSGDKGAGK
                     SLVLRTAIHSLKENPITWLFGECSNTSQISPCGLIQNILLNFFNITNFCSDGLKLKKD
                     SQKFFHSEFPMLSNDEVFALLNFLYPTSGDYFENILINKEKTFTFLKKIFQTIIDNSE
                     TVFVIENFEMIDGMSYEFLYRLLEADFISQRFKILLTYNELRPAKGYFYNKNINDNNY
                     VDLSLSPFDLAQSKTFINQYLKESSTCTEELKDNIFELTKGNPAFLEQVCSFMNEVYE
                     KWQNYNFNYPYSFNETVSIRLDNLKQENSVALEILAAASIHGTRFYPSILNQIFELSE
                     DEFVSHLIELQKRNFISKVNEFTYEIKNSMLWEAIFEYFKGSETIAIFNEKLFSIYSI
                     YTLSSASSIAVIAQNLNQKLAALNIWTEVIKLAAYIGDVNLYVIAQKQCLNFVDQIEK
                     TNSSLIKNNIYERLGKVLAQTNPKEAMLYLPAAVANAKNLETPIKQIELTAYLASCCM
                     TLGDYYGTIECVNSVLERLDFNYELEIAILKSRKLDALLKIGNCGEIVNLIDNEIMPV
                     FDKYISSKSHKNVSIQKLYAAWLKSYLILANALVFQGNNRAFEVVSTLFDIMQKNNFN
                     EPIFIAKVKLAAALAHTIRGEVEKSEDILEEIIKQNQSDNLDNEAISRWNLINILNNF
                     ILKKYNGLKEELFDVVTFANNVNDNFTKNILKTILGKILKDEENTVRATEIYNDQITF
                     FAKEKNAIGALLTWYFIADINLITEGPEKAIDVALKALDVAQSTKINNYYFITLYNKI
                     LAEAYIMQAEFELAKIHIEKAVMVARKFELLDLLARLYHLYGKYLQELAIGKSDSQVD
                     FVTGAYKMYKKATALSQTLKNNYVLEEIASSENKLNAFCRLNGIVIK"
     gene            complement(20859..22091)
                     /locus_tag="PKI94_04940"
     CDS             complement(20859..22091)
                     /locus_tag="PKI94_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNW26120.1"
                     /translation="MKKQVIIFIFLVFALLLFNNFLLKQFSYNSAMISSKDLVDKRQI
                     NSQKLFENSWRIIKNNFYDSSMNHQDWKRWKKHYKGKIKTDEDANVAINTILASLDDP
                     YSRFLNQKEYADQNISIDSKISGIGVNIASLGGKTYVVSVIEGTPAYKANLKVKDIIM
                     KVDGKDISGLEISEVASLVRGKENTTVNLVILRGKKKIQKNIIRKEIKIKTVKSSVEK
                     NIGYIQILSFIGSETSEEFIQALEKTQNTDGLIIDLRGNTGGLLPNAVFIANMFIDKG
                     NIVSVVGRNGYRQRFYAQQTDFVIDKPLLVLVDGGSASASEILSGALKDYHKAKLIGT
                     KTFGKGMVQKIIPMPNMTGINLTIAKYLTPAGHDINKIGIEPDIKVDLTQKDILANDD
                     VQLDTAKAMMKKMLEKIK"
     gene            complement(22242..22949)
                     /locus_tag="PKI94_04945"
     CDS             complement(22242..22949)
                     /locus_tag="PKI94_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26121.1"
                     /translation="MKKIKILTLLAAIAISTNVNQSVFSAQKKNPEPVKQEKEIVKAN
                     YPSKYTQEYILSLKDTYKSSSKDEVIYVALDMLKGTNGEFSREAVLGKNLTSKPIKIE
                     FKDLGSIKQDYANFDALGWKRGSRLYIYINQKHSDAPPMALAALLSHEALHQDEYNSL
                     AEETYAWTMEAAVWTELCDLHPEFESNFQPLVKREDTLKKLFEKGNYTNKYIKKAVFS
                     NSGYQNLPETSPGFEAL"
BASE COUNT         7679 a         3919 c         4166 g         7278 t
ORIGIN      
        1 ggacgcttgt cctgttagcg ttatcgttat tgaataatcg ataataaaaa acttaagggc
       61 gagttttccg aaatttcgga aaactcgccc ttatttaacg aatatggaag tttgcaatga
      121 atccaaaaat atctataatt gttcctgtat ataataatga aaaatttttg tccgaatgtt
      181 tagacagtat ttgccggcaa acattttctg atatagaaat tatttgcata aatgatggct
      241 caacggacaa cagtttaagt attcttgagg aatatgcgtt aaaagataaa agaattatct
      301 tattaaatca agaaaacaaa gggttgggct atactatgaa tcgtggcata aaaaccgcca
      361 gcggggattt tataggtatt gtcgaatccg atgattatat acttgaaaac atgtatgaat
      421 ccttgtataa aattgcagta aaaaatgatg ccgattttgt aaaatctaat ttttgtgtct
      481 tttatgatga agaaaattca agaaaattta ttcaggttcc tctttttgac gatgaaaaat
      541 attataatat cgttttaaat ccgaaaaaat caccgaatgt atttaacgct ataatgaaca
      601 attggacagg aatatataaa aaagattttt tagaaaaaaa taaaatttat catcttgaaa
      661 cacccggagc ttcatttcag gataccggct tttggtttaa agctttctgt tgtgccgaaa
      721 gaattttttt cttagacaaa gaattttata tgtgcaggcg tgataatccg gattcttcag
      781 taaacagtaa agaaaaggtt ttttgtattt gtgaagaata ttctgagatt gaaaattttt
      841 taaattctcg tcctgattta aaaaaatact ttgaaaaaat taaaaatcac aaaaaatttc
      901 aaatgtatca atggaatttt accagaatgg gttcaaaata taagcaaatg tttattaaaa
      961 atgcaagtaa ggaatttaag ttagcgcaaa aaaaagggat gttggacaaa aatttatttg
     1021 gagcaggaga atggaaaaag gttcaatttt taattaaaca tcctgttttg ttccctttaa
     1081 aatataatct tttaagtcct tatacaaaaa aaagaaaaga attaaaaatt cattttaaaa
     1141 aatattttga ttgtgttagg gctcaaaaac aaatcaacaa attagctcga aaatataaaa
     1201 ataaaaaaat tgttctctac ggcgcaggaa tttactgtaa aacacttttt gaaaattatg
     1261 atttgtcaaa tttgaatatt gtcgcagttg cagacagagg atttaaaaat gaagaaaaca
     1321 aaaacttctt taatttgaat tgtatattgc ctaaagattt gaaagaattt gattgcgact
     1381 taattttgtt gggtgtgtat gatactttac aaatttttga ttatttacaa gatcaattac
     1441 ttgtaggcag taaaaattcc caaataaaaa tcttgccgat agttgaagat acagtcattt
     1501 caagaattaa gaactttttt tcataataaa ttctatgatt tcttttgtgg aataaaaatc
     1561 attaattaca ataaaattaa aaccggtttt ttctttgaca taatcaagcg ttttgccgtc
     1621 taaaaaatcc tcagtgtatg ggcgcagcat tacggaggga attattacgg ttaagagtga
     1681 agagtgaagg gtgaagggat ttgtcattac tgaatttatt aaatcttcag aagttattag
     1741 tcccgcaacg gttatatttt caccccagta atcggattta accgcaacat aatcagcctt
     1801 taagtttttt actttattca actctgtaca aatttctttt atagcaggct ctgcaagctt
     1861 tgacgttgca aaaagaatat tcttaggctc gctaaggctt ttactaagtc gctttttttc
     1921 ttttccaaaa tcatcaagca aaagccttag cgcgccaacc ccgtcttcaa gctgagaaaa
     1981 attaccgtaa tatttgcgtg gcggaatatt tgcttcagct aatgaaaaaa attcatccga
     2041 acagcaaata gttaaatttg acttagttat tccccctctc cccttgcggg agagggcagg
     2101 ggtgaggggt acattgataa attcatcaac tatcttaatt gtttctatag ctattttttt
     2161 tgtaaccgga tataatttac ctttttgaaa tttagtgatt ccaacaggta caatcgcaat
     2221 tgaaacaacg tttttaagtt ttttcaagtc aaataatgtc ctttttaatt cttctccgtc
     2281 attgtatttc ggacaaagca caatttgagc gtggattgga atttcaattt tttcaagcca
     2341 tttaagatgt tttaagatat ctgctgcttt ggggttttta agcattttta cccttaattc
     2401 gggatttgtt gtgtgaacgg aaatatataa aggccccaat cttaattttt caatcctttt
     2461 tttgtctgct tcgctcaaat ttgtcaaggt tatgtatgtt ccctgcaggt aagaaagtct
     2521 gtaatcatca tctttaatgt acaaagtttc tctcaaaccc tcgggttgct ggtcaacgaa
     2581 gcaaaaaata catttgttgc agcagggctt aactttgtca aaaactgcgc tttcaaaaat
     2641 tattcccaaa tcatcgtcaa aatctttttc aatttcgatt acttcgattt cgccgtttat
     2701 ttttttaatt tcaattgtaa caagttcgct tttgcacaga aaattatagt caatcatatc
     2761 agacattttt tgctcatcga ttgacaaaat ttcatcgcct gattgtattt caagttcatg
     2821 tgctattgag tttttttctg ttttgctaac tattgcaggc attttatact ttattatttt
     2881 ctttatctaa actttcaatc agagaaatca gattattata ttcaacaatg gaattatcca
     2941 aaataatccg ttgataaaag ttcagggaat tgtaatcgtt tgcaagaatt ttttctgcgc
     3001 tgtgtttgtg ttcaaaagcc ttatttttaa ttaatgaaaa caaaattttg cgctcgtctt
     3061 ttgcaagata cgaagcacaa catagcttaa ttctggcatt tgaagcaaat tgaagcggat
     3121 tgtccgataa tttttcaagc aaaagttggg ttaatttttc tctgccttcg cttgtgacag
     3181 agtaataacc cgataatttt ccgccttccg agattgcttt tctggaacgg ataacccctt
     3241 ctttttcaag ccggttaagt gcaggtttta tcgcaccgaa gctgggttgg gaaaatgccg
     3301 caaaattatc cagtatcgcc tttaaaacgg catacattgt catttcccgc tcagataaaa
     3361 catacaaaat cagcaactca atcatatgtg tatattagca taaaaataac aggtgcggaa
     3421 tttttttaaa aaaattccgc acctgtttta caaaaaaatt atttgattta taatcaagct
     3481 actatcatac aagaaagaag tgaaatattt tgacaaagac attgacaaaa caagatgtag
     3541 ctgaaatcgt taaaaaaaca aatcttcagc atatagcaat aattatggac ggaaatcgcc
     3601 gttgggcaaa gaaaaaaaat ttaccctctg ccataggtca taaaaaaggg gttgattcgc
     3661 tcaaaaccgc acttaaatcc tgtcataaat tcgggattaa atatttaacg gtttacgctt
     3721 tttcaaccga aaactggaac agaaagcagg aagaagtcga ttttttaatg gatttgttgg
     3781 ctcaaacaat caaaaaagaa cttaaggacc ttcataaaaa cagtgtggtt ataaaattta
     3841 tcggagattt gtcaaagctt aaccctaaac ttcaaaaaat attatttaac gccgaagaag
     3901 tcacaaaaga caacacgggc gtaaaccttc aaatagcatt caattacggt tcaagagatg
     3961 aaatcgtaaa tgccgttaaa aatatttcac aaaaagtttt aaacaaagaa attgcacccg
     4021 ataaaattac cgaagaaatt atttcaaaag aattatacac aagcaacatt cccgatcctg
     4081 atttgcttat cagaacaggc ggagaaaaaa gaatttcaaa ttatcttctc tggcagatag
     4141 cttattctga aattcttatt acgggcgaat tttggcctga ttttgacgag cagtcgatgg
     4201 aagaagcaat tttggaattt aaccgtcgtc agcgacgctt tggaggttaa gctttgttta
     4261 agatagttct tgcaaccgca aacaagcata aagtggaaga aattaacgct atttgcgagg
     4321 ggtttggcat aaagtttatc atgccgccaa aaggctttga ccccattgaa gacggaaaga
     4381 tttttgaaga aaatgccctg attaaagcaa aagccgccaa taaattaaca ggtcttcctt
     4441 cacttgcaga tgattcggga ctttgcgttg acgcgctaaa aggatttccc ggaatttttt
     4501 ctgcaagata tgcaaaaact caaagtgaaa aaattaaaaa aattctagcc gaaatgaaaa
     4561 atattccgaa tgaaaatcgc ggagcaaaat ttgtctgcgc aatggtttta cttgataaag
     4621 acggtaaaat tcttttccaa acaagaggcg aatgtcacgg aaaaatcgcc cttgaggcta
     4681 aaggtcaaaa cggattcggg tttgatccca tattcattgt aaaagggggc aattccacta
     4741 tggcggaact gagcgaagaa gaaaaaaata aaatttctca ccgtggaatt gcccttcaaa
     4801 aagttcttaa ttatactaat aaaaaattaa tataattaag gtatcttttc tcttgatttt
     4861 atgtgttttt gtgcgataat ttaattgtga taaaaatcac gctaaaaaca tttttatgaa
     4921 taaagaagga gaaaaagatg tcaagtacag acatagccaa agttgatttt gataaagtca
     4981 atgaaattat actctcatgc ggacctaaaa aatctaacgt aattgcgatt ttacaaaaaa
     5041 ttcaggaaga attcagatat ttgccagaag atgccctgac atatatcgga acaaaaatag
     5101 aagggctttc ccctgcgact gtatatgggg tagcaacttt ttataaccag tttagcttgg
     5161 atcccaaagg aaaatatatt atcagggttt gcgacggaac ggcatgccat gttcgcggtt
     5221 caatgccttt attaactgca cttaaagcaa aattaaattt aaaagaaggc aaatttacaa
     5281 ccgatgatat gatgtttacg gttgagattg taagctgtct tggtgcttgc agtttatcac
     5341 cggttattgt cgtaaacggt aaagtttatc caaaaatgac gacggatgct cttaaaatag
     5401 ttgtagatac tctgttaaaa caagaaagag gagaatagca atgaccatag tggaagtaaa
     5461 acaagaagaa gctaaaaaga acatagcggc acaaggtaca agaattttgg tttgtgccgg
     5521 aacaggctgt gttgcttgcg gcgcacttga aattatagaa gaatttaaga aaaaaggatt
     5581 agatgcatca actattcaat cagataaaat gaccgttgtg ccgacaggct gccacggttt
     5641 ttgtgaacag ggtgttttaa ttttaatccc tgatttagga attaattacg taaaagttca
     5701 aaaagaagat gttgatgaaa ttatcgatag tgttaaaacc ggtgttccgg tcgaaagact
     5761 tttgtatgtt gacaaagaca caggtgaaaa aattcaaaaa cgcgaagaaa taaactttta
     5821 taaaaaacaa aacagaacgg cactgggcaa tgtcgggaaa attaatgccg aagaaataaa
     5881 cgatgcaata gctttcagag gttatgaggc tttagaaaaa gttttaacaa aaatgactcc
     5941 cgatgaagtt atcaaagaag ttacggattc aggcttaaga ggcagaggcg gcggcggatt
     6001 tccgaccggt aaaaaatggt tctttacaag agtagctcaa ggcgataaaa aatacgtcgt
     6061 gtgtaacgct gacgagggag atcccggtgc atttatggac ggcagcctta tggaaggcga
     6121 tccgcataga ttgcttgaag gtatggcaat tgcagcttat gctatcggag cagatgaagc
     6181 atatatctac gtaagagccg aatacccgct ttctataaga cgtttgagaa ttgcgattaa
     6241 gcaagctgaa gaaaacggat ttttaggcaa gaacatttta ggaacaggtt ttaattttga
     6301 aattcatatc aaagaaggtg caggagcttt tgtttgcggt gaagaaacag ctctaatcgc
     6361 ttcaatcgaa ggcgaaagag ggatgccaag atcaaaacct cctttccctg caaacaaagg
     6421 tctttttgga aaaccgacac ttatcaataa cgtagaaact tacggaaatg ttccgggaat
     6481 tctgttaaac ggggcaagtt ggtttgccgc atttggaaca gataattcca aagggacaaa
     6541 aacattttct ttgacgggcg acattaataa cacaggactt atcgaagttc ctatgggaat
     6601 aactttaaga gaaattatat atgatatcgg cggaggactt cgtggcggca aaaaatttaa
     6661 agccgcacaa atcggcggtc catcaggcgg atgcttaaca gaagaacatc ttgatatgcc
     6721 tatggattac gataatctta caaaagcagg tgcaatggta ggttcaggcg gacttgttat
     6781 tatgaacgaa gacacctgta ttgttgaaat cgcacgtttc tttgcaagtt tcacccaaag
     6841 agaatcttgc ggaaaatgtg ttccttgcag agaaggtaca aaacaaatgc ttgcgattct
     6901 tgaaaaaata gtcgcgggca aaggcgaaat ggaagatttg gatgttttgg aagatttagc
     6961 tatagcggtt aaggacggtt cactttgcgg acttggtaaa acagcaccga atcctgtact
     7021 ttcgacttta aaatacttca gagatgaata tatagcacac attaaggaca aaaaatgtcc
     7081 cgcaggtgtt tgtaccgcga tgaagaaaat agttattaca gataaatgta tcggttgtac
     7141 caaatgtgca agaacttgtc ccgtggctgc aattacaggg aatgtcaaag aacagcatgt
     7201 tattgaccag gacaaatgca ttaaatgcga agcttgtatt ggcgtttgtc ctgttaaagc
     7261 gataattaag gagtaaatag gaaatagtaa atagaattga aaaggaaaca taaaaatgac
     7321 agaagataaa aaaacattat ttattgacgg aaaagaagta gaattcagcg atgaaaaaaa
     7381 tcttcttgaa gtaatcagaa aagcagggat gaacgttcct acattttgct accgccctga
     7441 tttaacagca ttcggtgctt gtagaatgtg cgttgtggaa attgaaggca gaggaattca
     7501 aacttcctgt acaatgccgc ccgaagcggg tttgaaaatt cacttaaata cagaaaaagt
     7561 aagaagaatc agaaaaactg ttctggaatt gcttcttgca aatcacgaca gagaatgcac
     7621 cacttgtgaa aaatcaggac actgtgaact tcaaaaatat tctgaagaat atggaattag
     7681 agatattaag tatccgacga ttaaaaaaga agacttttta cctcttgata catcaaatcc
     7741 ttcaattgtt cgtgacccta acaaatgtat cctttgcggt gcttgtgtaa gagcttgtgc
     7801 cgaattccat ggggataacc atgctatttt aggctttgca aacagagggt caaaaacaag
     7861 agttcaaccg atggcaggac gagatttttc taaaacagac tgcatcttgt gcggtcaatg
     7921 tcaggcagtt tgcccgacag gcgcattaac cgttgtttct gacgtggata atgtttggaa
     7981 ggaaattaac aatccggaga aaaaagttgt tgcacaaatc gcaccttcaa tccgtgtggc
     8041 tttgggcgaa ttgttcggac ttgaaccggg agaaaattcc atcggaagaa ttactgctgc
     8101 acttagaaga atcggttttg atttggtatt tgatacaaac tttgcggcag atttaaccat
     8161 tatggaagaa ggtaccgagt ttattgaaag attaaaatca ggtaaaaatc ttcctatatt
     8221 tacttcatgc tgtccggcat ggattaaata cgttgaacag tcaagacccg aatatataaa
     8281 aaatctttct tcatgcaaat cgcctcaatc tatgatgtca ccggttttga aagaactctt
     8341 gcctaaatat tttgaaggtt atacaaaaga aaatactgtt attgtttcaa taatgccgtg
     8401 tactgcgaaa aaatttgaaa ataaacgtca agaactttcg caaaacggta ttccagatac
     8461 gaatttcgta ttaactacaa aagaattggg aagaatgatt aacgaagcag gcatcgattt
     8521 caaaaacctt gaacctgaaa aagaagacag tccttttggg gattacaccg gtgctgcaac
     8581 aatattcgga gcatcaggcg gtgttgccga agctgctgca agaacagctt acgaacttgt
     8641 tacaggcaaa gaacttacag acgttaacct taaaccaatg agaggcgtta acgcgtccga
     8701 aagaagcaaa gaaatggaac ttgacttaaa cggactaaaa gtcaaagtca gaatagtttc
     8761 aacaatagct gaagctgaaa aagcacttaa agagcttgat aatggcacag ccgattttca
     8821 aatgcttgaa gttatggctt gtcccggcgg ttgcgtaaat ggcggaggac aaccgtatag
     8881 ctttaatgat actacaatta aagctaaacg tgcagcaggg ttatataagg aagatgctga
     8941 acttaaattc agaaaatcgc accaaaatcc tgatattaaa aaactttatg cggaattttt
     9001 ggaaaatccg ggctctcata aagcacacga aatgcttcat actcactatg aggataaatt
     9061 caaaggttct tacagagatt tgaaataatt taagaatagc cgaaaatacc aaagggcgga
     9121 tttgagcttt gctcaaatcc gccctttggt atcaaaaaaa attaattata aatataaaca
     9181 tcttgagcga gtttaatttc aaattcactt ccttcgtgga tataaacaga gcctccgcgg
     9241 gtaaataccg caacggcagg tgctgcaata atattggcac cgaaggcaac aacgccgata
     9301 aataaagaaa acgggacaag aataatgccg gaaccttcgc tggcaagatt tccgctcact
     9361 ctaatcattt taccgaagaa actgcagccg ggctttgcag cagaaacaat tcctttgcga
     9421 tattttcttt cgcctttaat gttattaaaa aaagtttttt taaaattaac tttactcaca
     9481 tacgcgtcta taggataagt ttttccgtca atattcattg aattaaagcg aattacaata
     9541 agcccgccgt ttcctccgaa ttgaggtttg tgtacatcaa gcatttcacc gttaaaaatt
     9601 gtaccggaag gaacagtaaa ataagttgtg cttacagggt atcttgaaac gatttttacg
     9661 ctcaaacctt ttcttgaacg gtcagaaacg gaattaatca gttttcccct gaattttgtt
     9721 ccttttttca aaacggcata agcctgggga tttgtctgtt ggtatgattt agcttgataa
     9781 tttgcctgaa cctgtgcctg ttgcttttgc tgtggctgag gcttagaagt ttgaaattct
     9841 ttttcgtcaa tttggtttaa ggttttgggc aaagggggca gagccatatc ctgttcgatt
     9901 tttgaagggt tataattttt tctgatttca tcatcaatgg aagtatcaag ttccaaagca
     9961 aaaacaggca tacataagat agtaaatagt aaatagtaaa tggtaaatag acgtaaaaaa
    10021 tacattctac ttttctcttt ccaacttttc aacttttcac ctcttcacct gttcacctat
    10081 ttaagcattt cattaatagc ttttgcggct tttttgcctg cacccatcgc atttattgca
    10141 tttgattcgc cggcaggtgc aacatcgcct cctgcataaa tttttgcaat agaagtttta
    10201 tttgtgtctt cttctattac gatatagcct cttttatcgg ttttaatctc aatcccttca
    10261 gcttgcgccg atttttgaat aatcgggttc ggacttgtgc ctagtgcgac aattatagta
    10321 tcaaaatcca tgtcaacaaa ttttcctgta gaaacaggtt ttcttcttcc gctttcatca
    10381 ggctctccaa gtttcataac ttcaagtttc atggatttga catggccgtc ttgaccgaaa
    10441 atttcaacag gggcgtgcaa taatttaaaa attactcctt cttctttggc gtgccttatt
    10501 tcttcaatgc gtgcgggaag ctctgcttct gttcttcgat aaacgatata aacctcttta
    10561 gccccgactc ttacggctgt tcttgcagaa tccatagcca catttcctcc gcctattact
    10621 gcgactttat caccgatttt aagcggagtg ggggtatgtt tttcgtgagc ttgcataaga
    10681 tttactcggg ttaaaaattc gtttgctgaa taaactccgt ttagattttc gcccttgatt
    10741 ccaagcattt taggcaagcc tgcacccgag cagataaaaa tagcgtcaaa tccgtcttct
    10801 tctaattgtt tcagggtaat tgattttcct acaataacat ttgttttgaa tttaacatcc
    10861 atttctttta acgcattgat ttctctgtct aaaaaatttc tcggaagcct gaaaggcggt
    10921 attccatagc gcaaaacgcc gcctaatttg tgcaaagctt caaatacaac aacttcgtgt
    10981 ccttcttttt ttaaatccgc agcagcggca attcctgcac agcctgagcc tacaatagca
    11041 atttttttac ctgaaagttt tatttcgggt gctttggcac gagtattatc tccgacatat
    11101 ctttccagcg cacctattgc aacaggtttt cccttaatcc ccaaaacgca gtttccttcg
    11161 cattgtcttt cttgaggaca aactctgcca caaaccgaag gcaaaaggtt tgttcttctg
    11221 ataatttcac ctgccaactc aagattatca tcttttaaag ctgcaataaa tcccgggata
    11281 ttaatattca cggggcatcc ctggatgcat tttgcatttt tacaatttaa gcatctttgg
    11341 acttcaagtt taacttgttc tttagttaaa ttttcttcta cggcttcaaa attgcttcgc
    11401 ctttttattt tgtcctgctc atgaggtctt tgtctttcta acatttaatc gctccctgac
    11461 ttcagtttac aaaaaaaaag tcttttgggc aaataattaa tcagaaatta atttccattt
    11521 tttagcaaca tcatccaaaa ttgtttttgt gtcttgcttt tcgagcaaaa tttcttggac
    11581 ggcagtgttt ataagcagat taatttcttt ttgccctttg ccttgtcgca aaactggctg
    11641 aactctgtta agctgccttg cgcttattac tcttgccttt gacatcaaat cgttttcgtc
    11701 atactttgtg tagaactcat ttcgcaatgt ctttttgttt gtggcaagaa catttgtcag
    11761 ttttgccagt ttaagctggt tttcatcgtt agttaaaaac agtgcaaatt ttaaggcatc
    11821 ttcttttttg gaagatttca gcgggattac cagattcata agcgaaaaat cgtactgccc
    11881 caatgcgcct gtaaactggg atgaaacatc ggtgttttta taagtggaag gcgcgttttc
    11941 cttaatcata ttcaaaaaat tagcccctgc ctgaaaaaat acaatttgat ttgacatgta
    12001 tttttccaaa acttctcttt gggtttgggt tatggtttct ttcgggatta aattcctgcg
    12061 gtaaagttct ttataaaaat caaaaacatc aactgctgcc tgagagttaa ttttgccggc
    12121 agaatttacg ccgtatttgt tcaaaatttt gagcattgta tcgttttctg taatggttgg
    12181 cataaaaacg taagcaccgg ttttttcttt tatatcgggc gcgattgtcc atatttcacg
    12241 gtaatttctt ggaaaaagtt ttacccccga ttttttgtac aaccctctgt tataaatggt
    12301 aatcgccgaa gttgcatacc agggaagagc atatagtttg cctttatatt ttagcgaatt
    12361 tataatctca gagttaaact gccctgtatt gctctcgtta atttctttca acgcgccttt
    12421 ttgagccaaa atcgcagaaa aatcaggatt taagtttatt aaatcaggag gattgtcgct
    12481 taaaatggca gcaagagttc ttttttcgcc ttctgagaaa ggaacatcaa cccatttgat
    12541 tttaatttcg gggttttgct tctcaaactc tgttatgacg ccgttcatat aaggcgcaaa
    12601 atctcccatt tgcaaagtcc agaaaacaag ttggttttct ttgggcgatt tttttgcaag
    12661 caaaaaaatc gcccctatga ccaatataat taaaactctg acaatatttt ttttcaaatt
    12721 atatctccct atttgtcgtt gcgaggagca gagcgacgaa gcaatccaac aatttccata
    12781 tgcggacaag attgcttcgg tggataataa tttttcaatc cgataaccct cgtctcgcaa
    12841 tgacgtaaat ttatatgcta caattatact atgaataatt tatttacgga actggaaaat
    12901 caaaataagc aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntacaatt
    13021 atactatgaa taatttattt acggaactgg aaaatcaaaa taagcaaatt ccgcttgcag
    13081 acctgcttcg cccgaaaaat atcgaagaat ttttgggtca gagcaacgtt atttcaaaaa
    13141 acagtcctct tttaaatctg cttaaaacaa ataggctttt ttccataatt ctttggggaa
    13201 cacccggctg cggaaaaact actctggcaa gaataatcgc ccttcagaca aattcacaat
    13261 ttattgagat ttcagccgta acttcaggtg taaaagacat taaagaagcg gttgaaagtg
    13321 caaaaatggc acttagaaac ggaacaaaaa caattttgtt tatcgatgaa attcaccgct
    13381 attcaaaaac tcagcaggat gcgcttttac ctcatattga aaatggaaca atttttctta
    13441 taggatcaac tacagagaac ccttcgtttc aggtaattcc ggcactgctt tcaagggttc
    13501 aggtgataag gcttaattcg cttgacgacg aaagtgttaa aaatattgtg ataaaaggtt
    13561 ttgattattt gagccaaaat tatcaaaaaa ttaccttcga tgaaaaaata acggatttta
    13621 taataaacca tgcaagaggt gacgccagag ttgcgcttaa tctggttgaa aattcctatt
    13681 ttgcaagcga tattgccgat aataaaagga ttttgagcat agaaacatta gaacaaattt
    13741 ctcaaaaaag aagtaccaga tattcaagag atgagcacta tgactgtgct tcggcgtttc
    13801 aaaaaagttt gagaggctct gatgccgatg cttcgattta ttatcttgca aaaatgatag
    13861 catcaggcga agatccgcgc tttatcgcaa gaagattaat agtgacagcc gcagaagatg
    13921 tcggaaacgc cgatacaaac gcacttgcag ttgcaattaa tgcttataaa gcggttgaac
    13981 ttttggggtt tcccgaagca agaattccgc tttcgcaagc ggtaatttat gtcgccaaag
    14041 caccaaaatc aaacacaacg gtatgtgcta tcgaccaagc actttctgat atagataacg
    14101 gaaaagactt tccgccgcct atgcacttga gagattctca ttataaagat gcttcaaaat
    14161 acggcttcgg gcaaggctat gtctattcac actctgcgcc cgagcagtac cagcaatttt
    14221 tacccgatga acttgtgggc aaaaaatatg ttaaagacga acccaaatta aacgtttgcc
    14281 ctaaaaataa agactacaaa aacgaattgg gaaatattta acaaacaaag gcggcaggtg
    14341 atgaaatcac ctgccgcctt tgttttattt tttaacctat taattcaaat atttccattt
    14401 ggttattttt tttagttttg cttattgatt ttacaatttc aatggctttt ttaaccactt
    14461 tatcatcaac ccaatcacct gcttttgcaa cggcaacttt tgctttttct agtatttttg
    14521 catcttcggg cagtgcttta tttaaattaa cttcttttat tgcaggaagt aattttcttg
    14581 ctgcaagtgc caagccgcct ataatcacag catctttgat taaggtccaa agaaaatgtc
    14641 caaacccgcc ttttttctta ggtgtttgtg ccgtaggagc tgcgcctgtg tcagggccca
    14701 catacaatcc ctgagattgc aacatttccg gtgaaagctt atccattgct tttgccgaag
    14761 taaaatttac acctgatact gcattaacca taagaaacct ccaattttaa aacactatat
    14821 atttaatata agtatttttt caagcctttt ttgtttgcat tttaggcaat aaagaaataa
    14881 tgttacaaaa attaacacgt tttttggaat attattttaa tgtttgtaat cttacatatt
    14941 agaggggata aaaatatgag catattaaat aaaaacatcg ttaagataac atcagaagac
    15001 ttgaaggatg caaccttatc ttcggcgggt tttgacggag aaacccaaaa acgtgctttt
    15061 gcaaatatct taggtgcaag gcttgcgata aaacttcttt tttcaaaaaa aattgaggca
    15121 aataacattt acagccttta tacaatgcaa agcgtattaa aaaatattga tatatccgat
    15181 atttattttg aaaatataaa aattgatgtc agattggttt ttgacaaaaa tgagattttt
    15241 atacctaaaa gccattttga atatgatatt ttgcctgata tttatatacc tctattgtta
    15301 aaaaaagata tgtcttcggc tgaaatttta ggtttttttg agcccaaaga aatagataag
    15361 aataattcaa ataaagattt ttattttttt gaagcagata aacttcataa acctgaaagt
    15421 ttgaaaaaat ttatcagcct ttttattcaa aaaagtccaa aaaaatccaa attggaagac
    15481 ctgcaaaaat ctgaaagtct ttttggagaa tttgttgacg atgaaatatc tgatgaagat
    15541 aaattgttct taattcagca attaaacgcc aatttatatc tgagagaaaa attagtagag
    15601 ttggaaaatt ttgaattgct ttctaaaaaa tctatatcag aagaagtttt ttcacacgat
    15661 atgttatttg aagaaggcat taacgaagaa attccggaag aaaaatctct tgaaatttca
    15721 gaagaagaat cggaaataaa atcggaagat tttcttgagc tgccttcaga aggcgtcttt
    15781 gaggagcaat tacatgaaga gctgacagag caggatttgg aaaatattca agaggaagta
    15841 ccttttattg aggaatttga ttcggcttta cctgacgaaa aacaagagcc tgaaagtgct
    15901 gtccaagaag aagaaattaa agagcaaaat tatcaaagtg aattggtttc tttagattct
    15961 ttggtaggcg aagaattgtt tacagagggg ttgcccgaaa aacaagagaa tattgatgtc
    16021 ccgcttgaag agattgattt ttcccaattt gaatctatag gcgaagtaca atctgatttg
    16081 gcagagaaaa atgaagattt agatgcagaa gcagaagagc ttatttcgaa aaaaaacgct
    16141 gtatccgaat cggaagaaga cactgatgat gaatccataa aatcaaaaat agacgaactt
    16201 ttagaggagt tggaaacttc tgaagaggct aaagaagcac tttctgatga gattgaaatt
    16261 atagaaggtt tggtttcttc aagtgaaggt aaatcatctg aaagttttga tttgtcagat
    16321 tttgaaacag tttcaagtga tgaattggaa ttctttacac cggaagccga aactgaagca
    16381 gaagcggaga aaaaagacaa agatttaata aatatccttt ttgaaaaaga gaatataaaa
    16441 gccacagatg atatggacag cttgtcagag ccttcggttc ataaaaaagc ttataacata
    16501 aatattaaca aaaaggttat tgttgctgct tccattgcag gattgatgct tgttgctaca
    16561 gtaggaggag ttttgattac aaaacataat tcccaaaata atagcattgt tcaaaatgaa
    16621 attaatcaga atattccgcc tgcagctaca gataatacaa tgccacaaga cttcacacaa
    16681 aacgcctatc agcccgacgg caatatgcct ttaacacctc ctcccggcca gtatcagtct
    16741 caaggtcgag atatgagcaa agctgtttct gatgcatttc tttcaacacc gattaatgct
    16801 accgtttcaa aaattgcctg ggaagcaccg gaagatcttg catatagcga cagttttaga
    16861 aaatacttgc aaatggccgg caaaaatctt aaacttacac tccaaaatga tttattgttg
    16921 tcttctgaaa tggcttattc caataaagtc attgtcgata tatctatgaa taaaggtggg
    16981 acgataaaaa ccgctaacat tagcgtttca agcggttcaa aacaaattga taatatagtg
    17041 ttacaaagtg ttaaagagac tttaaaatac ctaaaaatgc ctactgacga gattcaaggc
    17101 tcttctgtag atttaaccct tatcataaac ttctaaatgt ggtatcattg tgctatagta
    17161 ttgtagggtt atctgtacat gtagaaagac tttgaagtgg aattagcaca acaaaaggtc
    17221 gatttaataa aaaagattat taaatcggac aaaaagtact taaataacga ggatttgttt
    17281 gaagactttt ttaatgagtc ttataagcgt tcgtttttgg ttgcgaaaac agttgaaaat
    17341 gaaaatgcgc ttgaaactta cttaagaaaa attattacca cttcaattgt tgttgtgctt
    17401 aaagattcag gcagaattca aaggacttct gaggggtttg ttcccgtaaa agaggagcct
    17461 ttggaagtag tatcccagcc gattattgag aataaatatt caaatataca agtgtcctat
    17521 gatataatag atttaagcga aggccccgaa gagataacca taaaaaaaga aaccttacaa
    17581 acactggtcg atgcggttaa agccgcacat aaaaaaaatc cttcaaagca gtatttggag
    17641 ctttatgatt tgagatattc aagaggctta aagcaaaagc aaattgcaca ggagcttaat
    17701 ctgtcgcaat cggaagtttc caaaaggctg ttagagctta tggagcacgt aaaatcaaaa
    17761 ttcaatcaag attaattttg gttgcgggtg aaacttatga tatgtcccaa atgtaaaaga
    17821 gaaatacctg aaaattccct aaaatgcaat cattgcggtt caaggattgc gtctttgtgc
    17881 aaaaaatgta atgcttataa ttctatttat aatattaaat gcacaaattg tggcgaagag
    17941 ttgttaaagg tttgtccttc ttgcaagggg attaacttac ctactgctca aaaatgcaga
    18001 aaatgcggga ttgattttat aaataaaaca gtgcaacctg ctcctgtagc aatgcccgaa
    18061 gaaaaaagcc agcttgaatt tcaagaagca aagcctgaag taagtcaaga aattcctcaa
    18121 gaaattaaaa ctcacaaagt tgaagaagta actgaaaaaa ttcaatacag tgctaatttt
    18181 caaagtcagc aaaacgcgaa agattcactg gtaaaggctc ttatatcagg caattccaaa
    18241 ataatttcgc taagcggaga caagggtgca ggaaaatctc ttgtacttag aactgcaatc
    18301 cattccctga aggaaaatcc aataacctgg ctttttggag aatgttcaaa cacttctcaa
    18361 atatcgccat gcgggcttat tcaaaatatt cttttgaatt tttttaatat aacaaatttt
    18421 tgttcggacg gattaaaatt aaaaaaagac tctcaaaaat tttttcattc cgaattccct
    18481 atgcttagta acgacgaagt ttttgcgctt ttaaattttc tttatcccac aagcggggat
    18541 tattttgaaa atattttgat aaataaagaa aaaactttta cgttcttaaa aaaaattttt
    18601 caaacaatta tagacaacag tgaaactgtt tttgtaatag aaaattttga aatgattgac
    18661 gggatgtctt acgagttttt atatcgtctg cttgaagctg attttatttc gcaaagattt
    18721 aaaattttgc tgacttataa tgaattaagg ccggcaaaag gctattttta taataaaaat
    18781 attaatgata acaattatgt tgacttatcg ctttctcctt ttgatttggc acaaagcaag
    18841 accttcatca atcaatatct gaaggaaagt tctacttgca ccgaagagct taaagataac
    18901 atttttgaat taacgaaggg aaatcctgca tttttagagc aggtttgttc ttttatgaat
    18961 gaagtttatg aaaaatggca aaactataat tttaattacc cttattcttt taacgaaacg
    19021 gtttctatca ggcttgataa tctgaaacag gaaaattcag ttgctttaga aatacttgcc
    19081 gcagcttcaa ttcacggtac aagattttat ccttcaattt taaatcaaat ttttgagtta
    19141 agtgaagatg aatttgtttc acatttaata gaattgcaaa aaagaaattt tatctcaaaa
    19201 gttaacgaat tcacttatga aattaagaat tcaatgcttt gggaagctat ttttgaatat
    19261 tttaaaggta gcgaaactat tgcaatattt aatgaaaaat tgttttcgat ttattctatt
    19321 tatacgcttt catctgcttc ttctatcgca gtaattgctc agaatttgaa ccaaaaactt
    19381 gcggctttga atatatggac tgaagttata aagcttgctg cctacattgg ggatgtgaat
    19441 ttatacgtaa tagcccaaaa acagtgctta aattttgtcg atcagattga aaaaacaaac
    19501 tcctcgttga ttaaaaataa tatttatgaa agattgggta aagttttggc ccaaacaaat
    19561 cctaaagaag caatgttgta tttacctgca gccgttgcaa atgcaaaaaa tcttgaaacc
    19621 cccattaagc aaattgaact caccgcctat cttgcaagct gctgcatgac actcggcgat
    19681 tactacggaa caatagagtg tgtaaattct gttttggaaa ggcttgattt taactatgaa
    19741 cttgaaattg caatacttaa atccagaaag cttgatgcgc ttttaaaaat aggaaactgc
    19801 ggggaaatcg ttaatcttat cgacaatgaa attatgcccg tttttgataa atatataagt
    19861 tcaaaatctc ataaaaatgt atctatacaa aagctctatg ccgcatggct gaaatcttat
    19921 ttaattctag caaatgcgtt ggtttttcaa ggtaataacc gtgcctttga agtcgtatca
    19981 accctttttg atattatgca aaaaaacaac tttaatgagc cgatttttat tgcaaaagtc
    20041 aagcttgcag cagcacttgc ccacaccata agaggtgaag tggaaaaatc ggaagatatt
    20101 cttgaagaaa taattaagca aaatcaaagt gataatttgg ataatgaggc tatttcaagg
    20161 tggaatttaa taaacatttt aaataatttc atcctaaaaa aatataacgg gcttaaagaa
    20221 gaactgtttg atgtcgtaac ttttgcaaac aacgtaaatg ataattttac aaaaaatatt
    20281 ttaaaaacca ttctgggcaa aatcttaaaa gacgaagaaa atactgttcg cgcgacagaa
    20341 atttataacg accagataac cttctttgca aaagaaaaaa atgccatagg cgcacttttg
    20401 acatggtatt ttatagcgga tataaacctg ataacagaag gacctgaaaa ggcaatagac
    20461 gttgccctaa aagctcttga tgttgcgcag agtacaaaaa ttaacaacta ctattttata
    20521 accctttaca ataaaatcct tgccgaagca tatataatgc aggccgaatt tgaacttgca
    20581 aaaattcaca tagaaaaagc agttatggta gcaagaaaat ttgaattgct cgatttgctt
    20641 gcaagacttt accacctcta cgggaaatat ctgcaagaac ttgctatcgg aaaatctgat
    20701 tctcaggtgg attttgtaac aggagcttat aaaatgtata aaaaagccac tgctctttct
    20761 caaacgttaa aaaataacta tgtacttgaa gaaatagctt catccgaaaa caaattaaac
    20821 gctttttgta ggcttaacgg aattgtaatt aaataatttc atttaatttt ttcaagcatt
    20881 tttttcatca ttgcctttgc agtatcaagc tgtacatcat catttgcaag aatgtctttt
    20941 tgagttaaat caactttaat gtcgggttct attcctattt tgttaatgtc gtgtcctgca
    21001 ggcgtcaaat attttgcgat tgtaagatta attcctgtca tatttggcat agggatgatt
    21061 ttttgcacca tacctttgcc aaaagtttta gttcctataa gttttgcctt atgatagtcc
    21121 ttgagagcac ctgacagaat ttcgctggca cttgcagagc ctccgtcaac caaaaccaaa
    21181 agcggtttgt ctattacaaa atcagtctgt tgtgcgtaaa acctttgtct gtagccgttt
    21241 ctacccacaa cgcttacaat atttcctttg tctatgaaca tatttgcgat aaaaaccgca
    21301 ttgggcagaa gtccgccggt atttcctctt aaatcaatta tcaaaccgtc tgtattttga
    21361 gttttttcga gtgcctgaat aaattcttcg gaagtttcag aacctataaa gcttaaaatt
    21421 tgaatatatc ctatgttttt ttctacagaa cttttaaccg tttttatttt aatttctttt
    21481 cttataatgt ttttttgtat ttttttcttg cctctcagaa taacaagatt aacagtggtg
    21541 ttttcttttc cccttacaag tgaagctact tcggatattt caagtccgct gatgtctttt
    21601 ccgtcaacct tcattattat gtcttttact ttaagattgg ctttatatgc aggtgttcct
    21661 tcaataacac ttaccacata agtttttcca cccaaagatg cgatgttaac gccgataccg
    21721 cttatttttg aatcaatgga tatgttttgg tcagcatatt ctttttggtt taagaatctt
    21781 gaataagggt catccaagct ggcaagtatt gtgtttattg ctacatttgc gtcttcatct
    21841 gttttgattt tgcctttgta atgttttttc caacgtttcc agtcttggtg gttcattgag
    21901 gaatcataaa aattattttt aataattctc catgaatttt caaataattt ctgagaattg
    21961 atttgccttt tatcaaccaa gtctttagag cttatcatag ctgaattata agaaaattgc
    22021 ttcaataaaa aattattgaa taacaaaagc gcaaaaacca aaaatataaa aataataact
    22081 tgttttttca tttgttcccc aaaaaccggc ttctgataga gcttgcggaa aaattgtcat
    22141 ttttcgaatt tttctgcgtc cttattacct aattatattt aacatcaagc ctcaactttt
    22201 atcaatagcc ttgttttaca tcagaaagaa caagttatta tttacaaagc ctcaaagccc
    22261 ggtgaggttt caggaagatt ttggtaacct gaatttgaaa aaacggcctt tttaatatat
    22321 ttattggtat aatttccttt ttcaaaaagc ttttttaatg tgtcttctct ttttaccaag
    22381 ggctgaaagt tgctttcaaa ttcaggatgt aagtcacaca attctgtcca aactgcagcc
    22441 tccattgtcc aagcgtaagt ttcttcggca agtgaattat attcatcctg atgcagagcc
    22501 tcgtgagaaa gcaatgctgc aagtgccata gggggtgcat ctgagtgttt ttgatttatg
    22561 taaatataaa gtctggaacc gcgcttccag cccagcgcat caaaatttgc ataatcttgt
    22621 tttattgaac ctaagtcctt aaattcaatt ttaataggct ttgacgtcaa atttttaccc
    22681 aaaacggctt cgcgagaaaa ttctccgttg gtacctttga gcatgtccaa ggcaacataa
    22741 ataacctcat ctttacttga agatttatat gtatctttca ggctcaagat gtattcttga
    22801 gtgtattttg acgggtaatt cgcttttaca atctcttttt cttgtttaac aggctcagga
    22861 tttttctttt gtgccgaaaa aaccgattga ttcacatttg tactgattgc tattgcagct
    22921 aaaagtgtta aaatcttaat cttcttcata atatccccta tctcttccat atgacatttt
    22981 caaaaaatgc cgtgagcttg cggaaaaatt aaaatttttc tcgcaatctc ccttgtccgg
    23041 cttgcaccca taccgccggc tatcccgcag ccggtatggg tgcaagcact tctaaggtcg
    23101 gatttgaaaa attaagaaaa attgtcattt ttcaaatttt tc
//
LOCUS       DAOQAU010000024        23072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2235_length_23072_cov_6.722640, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000024 DAOQAU010000000
VERSION     DAOQAU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 23072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23072
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_23072_cov_6.722640"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PKI94_04950"
     CDS             <1..1512
                     /locus_tag="PKI94_04950"
                     /inference="COORDINATES: protein motif:HMM:NF020859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW26122.1"
                     /translation="SDSEDYTKLLPQYKYSSVNEFRTSLTDEEIKIFMGLLLHPNRIS
                     IGKAISLTKYNLTQKNQSFIPSDITFRRYAKWFKDNNYDQWVLMRDGEKSLTDKVEPY
                     IKRDASLLEVGDILVADGHKLNFQVINPFTGKPCRATLVGFLDWKSTALVGYEIMLEE
                     NTQCIASALRNAIINLDMIPKVIYQDNGRAFRAKYFTHDKGFNELGFNGLYSKLGIQT
                     VFARPYNARAKVIERFFKEFQEGFERLLPSFTGSSITNKPAYLMRNEKFHKAMHNEYI
                     PTVDETIKMIDMWLSFKNSQPCSNANGKTIQEVLDERQRQNIDINLLDDLMLMTEVKT
                     IQRNGIRFLGCDYFDERLYGLKSKVLIKYSMFDLRSIKVFTPKGEYLCTAERVTETHP
                     MAKILGTVEDLEDFKQKIEKQKALKCRTINSVKKYLPTEDIKFLEMQMHSEVEVMVGA
                     EASLPPLKEERKINGKPIFKNNLEKYEWLVKNEPDNIWIQEFQQTKEYKIYHE"
     gene            1505..2188
                     /locus_tag="PKI94_04955"
     CDS             1505..2188
                     /locus_tag="PKI94_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW26123.1"
                     /translation="MNKTFVKTKNVKNFIGLIENLLAKPANLPKMALVYGEPGLGKSQ
                     TALWLACQYDSVYIRCTNLMTGRWLLEEIVKELEEVPRYLTSDNFNLCVKNLKASQKI
                     VIIDEIDYLANDKKSIETLRDIHDKTNCPMIFVGMGLVNKKLERHRHLYDRFSEIVKF
                     ETFSVADVKEIVDKLAEVNFSAEAIELLHTKHNRFRQIAQAINQFETIARENCFTEIT
                     AEIAKEVLC"
     gene            2182..2685
                     /locus_tag="PKI94_04960"
     CDS             2182..2685
                     /locus_tag="PKI94_04960"
                     /inference="COORDINATES: protein motif:HMM:NF026010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26124.1"
                     /translation="MLEKLARRLKQFTIDEIAMYIENPDFETLIEAGKLKFDGKIYTY
                     INLPNLNFELIEKPELNVKSRILFKDVTKGFLADRKLTKNTLKGYKYQLKFNILQYFG
                     EKYLDEITLVMIKEFMHSLSLKYRPKTVSNGVTLTGSILKWAFENGLIEHNPYLGIKN
                     PRVRYEK"
     gene            2682..3104
                     /locus_tag="PKI94_04965"
     CDS             2682..3104
                     /locus_tag="PKI94_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW26125.1"
                     /translation="MNKLKLANKAYELYSIENLSAEEIAAKLGTSRRTVFNWMKKYNW
                     VKKRINIEEIKNNFGRDTEELALKMMKKISSDIDAGRKLSQEELYSVMNILEHLPALK
                     KSMKLPTLINPAKPKTLTPDMVAKMQRDILGLDFNRRL"
     gene            3340..12009
                     /locus_tag="PKI94_04970"
     CDS             3340..12009
                     /locus_tag="PKI94_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26126.1"
                     /translation="MNKDIKHLAIVLALCFLRSLIPQACFAENYTGGFTQLKSAISSS
                     TNARIFIANDNMVSTSALGAMSGTAGVNSVLTLNGANYNFNGAGFSGVTLVALRTLNV
                     NNFGSLNPDGSVLNSIKNFNLVGNGAFIYNNGGTVAINNSVFSSNTATNGGVFYNNLG
                     NSTITGSTFYNNSATSQGGAFFNAAGNFIIKNSTFTNNSSAKTISTTSTTAATSVLNY
                     GGAIYNGIISSVTGAITSAVNSEYTIGSTGFSNTFNNNFTLSTNSGSTTNYDNTARGY
                     GGAIYNSVALTSSVTGNNIGSRTSTTNINSNTFSANTNTAINSATTTNGTSYAQAYGG
                     AVYNLATATTSGAGTGTNTATNTTNIKANNFSSNQVKSEKTGSLSVTKSPARTSNSNA
                     YGGAIYNTTAASSSTTASINSNSTVATNIEQNSINNNNVTALNSSTANGYSATYSANT
                     NAYGYGGAIFNSATTTASGKGNASNTTNIKDNTSINSNSVVATNNSNNTTLGTSNAYG
                     YGGAIYNTTGVTNSGSGNAVNSTNISNNTTINNNSITATNSSNTTTGPSNAYGYGGAI
                     YNITTATSSATSAITSASNSTNTTDILSNDISNNFILSTNSSNTTTGISSAYGYGGAV
                     FNSLTATTNSTGTNSANNTANINNNNFNANYTTSENNGVTKTGDSYARAYGGTIYNTT
                     ASSASGTAGVNTSNVTTNIKNNTITNSSATGKNTGSITGLTAATRTNYAESAGGAIFN
                     IATAAASSNGINTANNTTNIQNNSFDNNSAIATNTSTAVGYSTSYLANTNANAWGGSI
                     YNSSTTTASGIGNATIILNITDNTSFGGNSATITNSSNPTKGSSSAYGYGGAIYNTTA
                     ASNSGTGTATNKTNISNNNLFSANSIAVTNSSAITTAGTSSAYGYGGVIYNTTTAGIT
                     GAGNTLNSTDISNNTFNDNFINSKNNGTAFGSNAYGYGGVIYNITTATASATSAITSL
                     AGTENITNILSNNFRQNYINTTNSNTTTGASNAYGYGGAIYNSLTATANSTGINTALN
                     TLNIESLFDTNSITVVNNGTTTNGNSYADAYSGAIYNITKATTSSTGTNTATVTTNIK
                     NSTINNNSAVGKNLGTITGLNAVARNNYAEAFGGAIYNNSTATASSTGINVTSNTTNI
                     TGNDFNDNFALALNNSTATGYSTSYLTNTNTNGYGGAILSIAAATASGTQTATNKINI
                     NENSFDGNYLSVTNSSNTTKGASSSYGYGGVIYSSLTSTASGSGITNTELSINDNSLF
                     RNNSLNVTNSAITTTGSSNAYGYGGAIYATSSVLNSGSGSANHLTNINNNVFDTNTLS
                     VINSGTTITGTSTARSYGGAVFVNTGLSTNLKNSASSTTNIKDNEFKNNSATATNSST
                     TTGTSNAYSYGGAIYTNTYTGNSGAVANSSVNTTNITGNTFEQNNAVATNSSTTIGTK
                     TAADSYGGAIYNTMSSTNSSTGVNSSSNTVSILDNIFKKNSANNGGAIFNTLSSTNSS
                     TGVNSSTNTLDIKDNRFEENTATYGGAIYNTWTSTGTNLSVNSVNITDNVFKLNTASY
                     GGAIFNTAGTLTVNGNTFDTNSAKYGGAIANYISTTTGSGSATINVDNGTFEKNFAKN
                     SSSTAAITSYGGAIFNYGYATTGTTNLNANVNNSTFDTNYAKAESTWASTSGYANALG
                     GAIYNYKSSTTGAGNSILDVENTVFKNNYTNASSSSGYANAQGGAIYNASAGTQVAKV
                     TIDGGTFNDNYVQATAKGNASSTYAYGYGGAIYNSGQLNVTDSSFNRNNANATGTTAS
                     TYAYGRGGAIYNTGTTTITNSTFTDNTAKSQGGAIYNTGTLKLVADNGTTSFTGNKAN
                     NISNALYLAGGTTYLNAGNGGVMTFNDKIASSAIGNVININQTSLGLVSGGEIIFNEN
                     VSDSTINLYSGMLTLGNTGNTRLTDSTGLNSSNFYTNNVKLNIYDAILNLANNNATDR
                     INLNSFTSTSDSSLQFDADFATNQNDMFTVSSIINPSNVLNTGLINILNDGLISSLTL
                     FSGGISPTLSAFSTFTTDYKYYFTPSTAGVYDVRRTTIAGLNYAIEDIAPTRSFSATR
                     DTYVDYNLGTMGGAVSTLDIFGNDKNIVGLGYSGANTLAGQTLNITNVGSLKSDGTVN
                     TSWNGFSSTSNGGGINNNGTVKITDSVFYNNTSTANGGLLYNANVATINTSAFVSNSA
                     ALGGAIYNSGTMTGLNDSFTSNYTTTANGGAVYNSSTVTLTSSTFKSNNALLGNGGAI
                     FNSGTFNLTGATFGGSSLSDANSAKNGGAVYNTGNIFLSGSLFKNNSSSVNGGAINNT
                     GTIQITNSIFENNTATSNGGAIYNSGTLKIIADGGTTSFTNNKASGASSAVYLASGSV
                     MNVNSGNGGAVTFNDEITSESGASININKINVSTNLAGTTFAPTDGKITFNENVNNST
                     INFYNGTMFLNKENLLDGNNVNLYGGSIDLINKSTGTASFNNLSINGVTNLAIDADLA
                     NGLADKITSANPITGTGSLYVNHVNTMGNSSAKSVSLSIADENVKDYVTVSPGCVMGN
                     ILKYNLNYDKSTGSLMFLSGGSSGDYKAYNPAILTSSINIQTGSYLNQANVYNEALGR
                     MDALMASSKYDRLLMKYQNKVASADGNFVFSPTFLQESSKGLWFKQYATFENVPMNNG
                     PNVSNVSYGMLIGGDTDIVSLKHGFDGYLTAYVGYNGSHQNYYTTGVYQNGGLAGLTG
                     TAYKGNFFTAVTLNLGGNNGNAYTTAGVDNFTTLVAGAAIKSGYNFEFLRGKLIVQPS
                     LMAGYTFANTFDYTTASGVNMTSDPLHVLQLAPGIKFIGNLNNGWQPYIAANMVYNIS
                     TGGTFVANDVQLPGMSIAPYVEYGVGIQRKWGDRLTGFAQTMLRGGGRNGIALQWGLR
                     WAF"
     gene            complement(12152..12493)
                     /locus_tag="PKI94_04975"
     CDS             complement(12152..12493)
                     /locus_tag="PKI94_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26127.1"
                     /translation="MKKILSITCVLSLLIGTRVFAAETYTGGLVDSLNQKINKVASPV
                     VNKEKEVKQKQKEMQELKQKQLEEQKKQAEARQKAQKELIDKKKQQIQTQKTLFNQQK
                     EEIKNIFKLDK"
     gene            complement(12557..15709)
                     /locus_tag="PKI94_04980"
     CDS             complement(12557..15709)
                     /locus_tag="PKI94_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNW26128.1"
                     /translation="MKKNLILTAKILSVLFCSIYVLFLILPLILSPIADSYKSDISEF
                     IEKESGFKVNLERIRIITTPKFTLGIKAGKTEIFLPNKEKFLQADNFQIKVSLLPVLF
                     KKIELDLISADNIDVVLKVQKKGKFLIENYIPKNKEENKELKSLPFKLSDNLPDVKIN
                     AHKITFVEALSGKKYSLSGCDTKITDFVFNKKIKISGKGEMILDERKQLDYDIKILNK
                     IMPKSHLNDLIFNPQPLEQKPQQFIFNIIDIFEALYSNKFNSNIKVDVKTYGDIEDVK
                     VDGFINIDKLSLAINNKKLPDSFILLKLKGNKIDLDCDFYTAEKEITHIKGVFKTGKK
                     PDIDMSFKSNLSLNNLVEIFKNITASFDINDFQTLSAGGYINADFRIKSNLKKILSSG
                     YFKIPQGSLKYGLYNVAINDINSDISFDNNIVNIKKTGFSILGNPLRIYGTIYQDAKA
                     DINIVAEKLAIKSLLLSLGQVSLLKENKFNSGDLSLKASLSGELTKPETAVNLYVNKV
                     NLKNIPTNSSVILSALNIKLKSDKKAYKGTADSVDFKIINPAAVIYVPKLVANIEPEE
                     VIISPSTVFIDKINLNVSGLIKNYLTDKIVLDIKTGGDIKSTLKGTVNSSSQKLNLNY
                     SIPRLCNFSIPGFANSKIQAKGDVVISGSMINPKLKGVFIIPYTYIPDMLVSMDDMVI
                     NLDGPIVKGNGTVKRIRSSGLVAQNLSSDFILKGDLFYLKNITGDAYGGKLKGDIVYN
                     IINGETGVDFQGYNMNATNAIAAASGIKNALSGTLGFDTKIKFRGIEYSDILKSLKGN
                     FSFKIEEGALGRVGSLESFLNAQNILANSVMRVALDTVASISTVKNTALFKYIKGDMT
                     FNNGWANISYIKTSGPTMSYFVSGRYNLLNGTTNVVVLGRLSSDVVALLGPIGELSVD
                     KLTSYIPKFGTLTAIIIKSMTTSPAQENVAQIPPLTGSNKYYKDFKVVFNGGIESKSS
                     VKSFKWLSDTDISAIDIKSTLTDVKKQFTDVKKNTVEEVKKQYSDVKNSTVNEVKNTV
                     NDTKKQLQDAAGEWKNLIKF"
     gene            complement(15771..16646)
                     /locus_tag="PKI94_04985"
     CDS             complement(15771..16646)
                     /locus_tag="PKI94_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HNW26129.1"
                     /translation="MKTQEKNRKKGHTKIFWDGVPATVRKSPLEDSLYNFLFKSVFGH
                     IAGIILICFFVFLLNFLGITPKIKDNVMKVKDIAFTLAGPANKGSVSQNNQNQVSSEP
                     ANKLKTSSMPQTKSVTQKIKSSGVSDFEIPIPDFKSMSSGLSSSKTSRIHASGLEVST
                     NSVSAAGGSSSSDSSSTGGGGFNKVATKNLIVAYDISPYVNELKREVRWSWKIPPTAG
                     GKRVELFLRIAKDGRLMILNVKRTSEIGAVDNAALDAVRKCMPLNPLPVKYNKSYLDL
                     IFSFDSATNSIGSRY"
     gene            complement(16624..18417)
                     /locus_tag="PKI94_04990"
     CDS             complement(16624..18417)
                     /locus_tag="PKI94_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26130.1"
                     /translation="MKEMGKKILIYTLGVFLCGSVAFAMPKKAYKHFIKANEYFAIND
                     TDNAEIEYKAAEELAPDELSICLKLATLYSNIQDWNNALLYYQKASKLNPDDGSIMLN
                     IAAVLRAQKDYKGAYEAYQKASQLLPNYTYIHLNLAQMQELMNQQKESIEEYKKFLIS
                     YPDNIDARKNLAALYLNLDMDTEAVGEYEAILAKSPDDFDDWTNYAKALLLTENFKKI
                     PEVVQKAMQDDPENVQLKIILATAYENTGEKEKAIVEYQSAFKMDPSLVEVQFNIAKL
                     SDELGREADAIKYYEACLDTLPDNSQAYYNLANIYQRKQEFNNSLSTLNKLKSKEKED
                     VKILKEIGKNYYMSGDFVQSINYYNEALKLSGDNAQIYYYMALSYTLMKEYDKAVENY
                     KKSISLQDDAKVKSELSKLYVTVANNYNQKNDYVSAVANFKEAVNLNKTDATLYCDLA
                     DALLLQGSTKEAIEAYKQALDLDKNNVDLMLSLANVYKNDSKPTEAQKYYEQVLTVDS
                     QNLDALFYIGAICFESGDSKKAKEYLIRATGAKPDFSNAYYLLGMICDKEKNYPDAIL
                     YYKKFITLSPKDESAIQIKKRLTLLENARKK"
     gene            18547..19596
                     /locus_tag="PKI94_04995"
     CDS             18547..19596
                     /locus_tag="PKI94_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNW26131.1"
                     /translation="MRLAIRNLVLSLFVIGLMASNAIAKSYPDLPENHWAYKQIQALT
                     DEDVLVGYPDGNFKPDETATRAEFTAMVIKALHQDKAPLKKTFDFTDVPYKHWAYNVI
                     QRAINFDLIKNTSDNTFRPEDTVTKKEAMDIMVSALNLDKWSLFRAQKAIGTFKNPDA
                     PITRAEMAFGLYNMQINARINPNKKLQEAMRAKRGEGYVLRGVTIDGTVATIPAGTTI
                     PVMLLTPLNSSVNKKGECFVTKANKHLVTKDRYIIFARASKINGEITSVTPGKLFVRT
                     GKMGLETKTIYTPIEQIACFPGCINTDQQRNWFMRIIRAIIKGGKVKLKAGKVVTITT
                     LKTIKVDLTSGWIIE"
     gene            complement(19654..20805)
                     /locus_tag="PKI94_05000"
     CDS             complement(19654..20805)
                     /locus_tag="PKI94_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNW26132.1"
                     /translation="MNIVKIQNTSNILFRGYRGTVKQVESNNKADDCINDSSFMRNLD
                     SLGFVCDYLKKEFPKGANIADFACSNGDETYSLGILLHDCNKDKKYKITGYDISTKAI
                     EDAEKGFLSLSYNYDGVVARVLTKDRYVSRLGQDNENWLKIKSNFNECFQKLGWDWEK
                     FNLQHPRAKKKVTKLPCASGDAEIDLARVKFIQTQNNFVKAGDYFVPKKGVFDDVIDF
                     KLADIVNIDKELTPNKTGVVFFKNALYHLMYQIKDDKYLVDFDIKPVKELFSKIYSML
                     SDKGIFVMGALNADHLFGRAVTADYFHKINQDGKELTVYDTSFVHQELREAGFEPIFY
                     EQDEKTVNDYLSPKVYLPSVWRKLSEEEIYQREQEQKKEKYAQIISLLT"
     gene            complement(20848..22161)
                     /gene="miaB"
                     /locus_tag="PKI94_05005"
     CDS             complement(20848..22161)
                     /gene="miaB"
                     /locus_tag="PKI94_05005"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNW26133.1"
                     /translation="MKKVYVETLGCQMNKSDTEKMLGMLSFMGYEEIKEPKKADLLII
                     NTCSIRQLSEDKAFSRIGLWGKWKKTRKNLKIAFCGCVAQQGGEKIFKRMPYIDLIMG
                     THNINEFPDLIKRIEAGEKVCATDETPLPEKDIKLKRAKGVNAWITITEGCDNFCTYC
                     IVPYTRGRERSRAPEVIIKEVKEALSQGFKEITLLGQNVDSYKGTEGYFLAHLLKDIN
                     KIEGTFRIRFVTSYPTDITDELIETVIKCDKICEYFHIPMQSGSSEILKKMNRRYDRK
                     KYFEIVKKVRDKVKDVTITSDFIAGFPGESEEQFEQTLSAIKELELDYCNTAAYSPRE
                     QTVAAKWKNLFLTEEVKDQRLARLNNQVKESALASNKKFIGREMEVLIEKFENHKGKN
                     IITGRTRNNKIVHIPFEKDLSGQFVNAKITGAKTWYLQGEFVNKN"
     gene            complement(22248..>23072)
                     /locus_tag="PKI94_05010"
     CDS             complement(22248..>23072)
                     /locus_tag="PKI94_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNW26134.1"
                     /translation="VQGLPRVEELLEGRKPKECSILAEDDGVAEIAIEDDTPTLYLVN
                     ENGRVEIKHTIDSNIIVADKQKIKKGQPLTSGPLNPHDVMRLNGPEVTQQYLVDEVQR
                     VYRSQGVEIADKHVEIIVRQMTKKVKVIEPGDTKLLAGELIEVQTFENANKETKEAGG
                     VEATCEYMLLGITKASLNTESWISAASFQETTRILTEAAVEGKQDLLRGLKENVIIGR
                     LIPAGTGYHHLTHASEEKDKEAQRRAAARNQARKPSAILEEIEGMFGTPEFALDEE"
BASE COUNT         7650 a         4296 c         3899 g         7227 t
ORIGIN      
        1 agcgacagcg aagattatac aaaactactt ccgcagtaca aatattcaag cgtaaatgaa
       61 ttcagaacaa gtttaacgga tgaagaaata aaaattttta tgggactttt gcttcacccg
      121 aacagaattt caatcggaaa agcaatttct ttaactaagt ataatttgac gcaaaaaaat
      181 caaagcttta tcccgtctga cataactttc agaagatatg caaagtggtt taaggataat
      241 aattacgacc agtgggtttt gatgcgagat ggagaaaaaa gtcttacgga taaagtggag
      301 ccatatataa agcgtgatgc aagtttgctt gaagtcggcg atattctagt cgctgacgga
      361 cataagctca attttcaggt gataaatccc ttcacaggca agccttgcag ggcaacttta
      421 gtcgggtttt tggattggaa gtcgactgct ttggtaggat atgaaattat gctcgaagaa
      481 aatacccaat gcatagcttc cgcccttcga aacgcaataa taaacctcga tatgatacca
      541 aaagtaattt atcaggataa tggcagagct ttcagggcaa aatattttac gcatgataaa
      601 ggtttcaatg aactcggctt taatggcttg tatagtaaac tcgggattca aacagttttt
      661 gctagaccat ataatgcgag ggctaaagta atcgaaagat tttttaaaga atttcaggaa
      721 ggatttgaaa gattattacc atcttttaca ggttcttcga ttacaaacaa acctgcatac
      781 ctgatgcgga acgaaaaatt tcataaagct atgcacaacg aatatattcc gacagtggat
      841 gaaacaatta agatgattga tatgtggctg agttttaaaa attctcaacc ttgcagtaat
      901 gccaacggaa aaacaattca ggaagttttg gatgaacggc aacggcaaaa tatagatata
      961 aatttacttg atgatttgat gcttatgacg gaagtcaaaa cgattcagcg aaatggcatc
     1021 agatttttag gttgtgatta ttttgatgaa agattatacg gactcaaaag caaagtgctg
     1081 attaaatata gcatgtttga tttgagaagt ataaaagtat ttactccaaa aggcgaatat
     1141 ctttgtacgg cagagcgagt aacagaaaca catccgatgg caaaaattct cgggacagtt
     1201 gaggatttgg aagattttaa acaaaaaatt gaaaagcaaa aagctctgaa atgcagaacg
     1261 attaattctg tgaagaaata tttgcctact gaggatatta aatttttaga aatgcagatg
     1321 cattcggagg ttgaagttat ggtaggggcg gaagcttcgc ttccgcccct aaaagaagaa
     1381 agaaaaatta acggcaaacc aatctttaaa aacaacttgg aaaaatacga atggcttgta
     1441 aagaacgagc cagataatat ttggatacaa gaattccaac aaacaaagga gtataaaatt
     1501 tatcatgaat aaaacttttg taaaaacaaa aaacgttaaa aattttatag gattaataga
     1561 gaatctcctt gcaaaacctg caaatttacc aaaaatggcg ttggtatacg gcgagcctgg
     1621 cttaggaaaa tcacaaacag cactttggtt agcttgccaa tatgacagcg tttacatccg
     1681 ttgtacaaat ttaatgacag ggcgatggct gttggaagaa attgtaaaag aacttgaaga
     1741 agtgccgaga tatttgactt ccgacaattt taatttgtgc gtaaaaaatc tcaaagcatc
     1801 tcaaaaaatt gtgataatcg atgaaattga ttatcttgca aatgataaaa aatcgattga
     1861 aacgctccga gatattcatg acaaaacaaa ttgcccgatg atttttgtag gaatgggttt
     1921 ggttaataaa aaacttgaac gacatcggca tctatatgat agattctcag agattgtcaa
     1981 atttgaaact tttagtgtgg cagatgtaaa agaaattgtg gacaaacttg cagaagttaa
     2041 tttttctgcg gaggcaattg aacttttaca cacaaaacat aatagatttc gccaaattgc
     2101 acaggcaata aaccaattcg aaactattgc cagagaaaac tgttttacgg aaatcactgc
     2161 agaaatagcc aaggaggtgc tatgttagaa aaattagcga gaagattaaa gcaatttacg
     2221 attgatgaaa ttgcgatgta tattgaaaat cctgattttg agacactaat agaggcaggg
     2281 aaactcaagt tcgacggaaa aatttatacc tatattaatt tgccaaattt gaattttgaa
     2341 ttaatcgaaa agccagaatt gaacgttaaa tcaagaattt tgttcaaaga tgtgacaaaa
     2401 ggatttttag cggatagaaa attaacaaaa aacacattga aggggtataa atatcagcta
     2461 aaatttaata tccttcagta ctttggcgaa aaatatttag atgaaatcac tcttgttatg
     2521 ataaaagaat ttatgcactc cctcagttta aaatatcgcc caaaaacagt gagcaatgga
     2581 gttacactta caggaagtat tttaaaatgg gcatttgaga atggtttgat tgagcataat
     2641 ccgtatttag gaattaaaaa tccacgagtg aggtacgaaa aatgaacaaa ttaaaactgg
     2701 caaacaaagc ttatgaactt tattcaatcg aaaacctttc cgcagaagag attgcggcaa
     2761 agcttggaac ttcaaggcgg acggttttta attggatgaa aaaatacaat tgggtaaaaa
     2821 agcggataaa tattgaagaa attaaaaata atttcggcag ggacacagaa gaattggctt
     2881 tgaaaatgat gaaaaaaata tcatccgata tcgacgctgg tcgaaaactc agccaagaag
     2941 aactttattc tgttatgaat attttggagc acttgccagc cttgaaaaaa tcaatgaaac
     3001 taccgacctt aataaatcct gccaaaccca aaactttaac tccagatatg gttgctaaaa
     3061 tgcaacgaga tattttgggc ttggatttta atcggcgttt ataagacgtt taatagccgt
     3121 ttactggcgt tttaaaactc gacttcacct gcaatgaact ctgtatgtac attgcaggtt
     3181 ttttgaagtc agcctaaaga ttccagtctt gttacattta tttgtggcat gaatgaatta
     3241 acaggcaaat agggaaatgt aaacaataat cgttatttgt tgccaatttg aaacgctttt
     3301 gttataatgt ttttaataac taacttgggc aacaggaaat tgaataaaga tatcaaacat
     3361 ctcgctattg ttttagcatt gtgttttttg cgaagtctta tacctcaagc ttgcttcgca
     3421 gaaaactaca caggtggatt tacccaatta aaatcagcta taagcagctc caccaacgct
     3481 agaattttta tcgccaatga caacatggtg tcaacttctg ctctcggcgc gatgagtgga
     3541 accgctggcg tgaacagcgt tttaacgcta aatggtgcca attacaattt taacggcgct
     3601 ggtttttcag gtgtaactct tgttgcattg agaactctta atgttaataa ttttgggtca
     3661 ctaaacccag acggcagcgt cttaaattcg ataaagaatt ttaacttagt cggaaatggt
     3721 gcctttatat ataacaatgg cggaactgtc gcaataaaca attccgtttt tagctcaaac
     3781 acagcaacaa acggcggagt tttttacaat aacttaggta attcgactat tacaggctca
     3841 actttttata acaacagtgc aacttctcag ggcggtgctt tttttaatgc agccggaaat
     3901 tttattatta agaattcaac ttttaccaat aactcttctg caaaaaccat cagtacaaca
     3961 tcaacaaccg cagctacttc tgttttaaat tatggtgggg caatttacaa cggcattatt
     4021 tcaagcgtta caggggctat aacgagtgca gtaaattccg aatatacaat cgggagcacg
     4081 ggtttttcaa atacattcaa taacaatttt actttaagta caaattcagg aagcacaaca
     4141 aattacgata atactgcaag aggctacggc ggagcaattt acaacagtgt ggcattaacg
     4201 tcctctgtta caggtaataa tattggaagt cgcacaagca caaccaatat taattcaaat
     4261 acctttagcg ctaatacaaa cacagcaata aatagcgcaa ctacaaccaa tggaacaagc
     4321 tacgcgcaag cttatggcgg ggcggtttat aatttagcca cggcaaccac ttcaggtgcc
     4381 ggaacaggaa caaatacagc aacaaacact acaaatatta aggcaaacaa ctttagcagc
     4441 aaccaagtaa aaagcgaaaa aaccggtagc ctttctgtta caaaaagccc tgcccgcacg
     4501 agtaattcaa acgcatacgg cggcgcgatt tataatacaa ctgcggcttc ttcctcaacc
     4561 acagcttcaa tcaattcaaa ttcaactgtg gcaaccaata ttgaacaaaa ctctattaac
     4621 aacaacaatg taacggcttt aaacagcagt acagcaaacg gctacagtgc aacttattcc
     4681 gcaaacacga acgcgtacgg atatggtgga gcaatcttta actcagcaac aacaaccgct
     4741 tctggcaaag gaaatgcctc taacaccaca aacattaagg ataatacttc aattaattcc
     4801 aactcggttg ttgcaacaaa caacagcaat aatacaaccc ttggaaccag caacgcatat
     4861 ggttatggcg gggctattta taatacaacc ggcgtgacta attcaggttc tggcaatgct
     4921 gtaaattcaa caaatatttc taataataca actattaata ataattcaat taccgctaca
     4981 aacagcagca atacaacaac aggcccaagt aacgcttatg gctacggcgg agcgatttac
     5041 aatataacga ccgcaacctc aagtgcaact tccgcaatca caagtgcctc taattcaaca
     5101 aataccacag atattctttc aaatgatatt tctaacaatt ttattttatc gacaaacagc
     5161 agtaatacta cgaccggcat aagcagtgct tacggctatg gcggagcagt tttcaatagt
     5221 cttactgcca ctacaaacag tacagggaca aattccgcaa acaatactgc aaacatcaac
     5281 aataacaatt tcaatgcaaa ctacactacc tctgaaaaca acggagttac aaaaaccggc
     5341 gacagttatg caagagctta cggcggtact atttataata cgactgcatc atcagcttca
     5401 ggaactgctg gagtaaatac ttcaaacgtc acgactaaca tcaaaaacaa tacaataact
     5461 aacagttctg caacaggtaa aaatacagga agcataaccg gattaacggc cgcaacccgt
     5521 actaattacg cagagtcagc aggcggagca atttttaaca ttgcaaccgc agccgcgtca
     5581 agtaatggaa taaacacagc caataacacc acaaacattc agaataattc ttttgataat
     5641 aactctgcaa tagctaccaa taccagtaca gctgttggtt acagtacttc ttatttggca
     5701 aacaccaatg caaacgcgtg gggcggaagt atttataata gctcaacaac aaccgcttca
     5761 ggaataggaa atgctacaat catcttaaac atcactgata acacttcttt tggcggcaat
     5821 tctgcgacaa ttacaaacag cagcaatcca acaaaaggtt caagcagtgc ttacggctat
     5881 ggtggagcga tttacaacac aactgccgca tcaaattcag gaacaggaac tgccacaaat
     5941 aagactaata tttccaacaa taacttattt agtgcaaatt ctatagctgt tacaaacagc
     6001 agcgcaataa caacagcagg cacaagcagt gcttacggtt atggcggagt tatttataac
     6061 accacaactg caggaattac aggagcagga aatactttaa actcaacaga tatttcaaac
     6121 aacacattta atgataattt tattaattca aaaaataacg gaacagcctt tggaagtaac
     6181 gcatatggtt atggcggagt gatttataat ataacaaccg caactgcaag tgcaacctca
     6241 gcaattacaa gtctcgcagg cacagaaaac atcacaaata ttttaagcaa taatttcaga
     6301 caaaattata ttaataccac caacagcaac accacaacag gtgcaagcaa tgcttatgga
     6361 tacggcgggg ccatttataa tagcctcact gccactgcaa acagtacagg gattaataca
     6421 gcattaaata ccctcaatat tgaaagtctt tttgatacca attcgattac ggttgtaaac
     6481 aacggcacga caactaacgg caacagctac gctgatgctt atagtggagc gatttataat
     6541 atcactaaag caacgacttc aagtaccggc acaaacacgg caacagttac aacaaatatt
     6601 aaaaatagca caataaataa caactcggct gtaggcaaaa atttgggaac tataacaggt
     6661 ttaaatgcag tagctagaaa caactatgca gaggcttttg gcggggcgat ttataataat
     6721 tcaacagcaa ccgcatcctc tacgggtatc aatgttacaa gcaacacaac aaacattaca
     6781 ggaaatgact ttaatgacaa ctttgcactg gcattaaata atagtacggc aacaggttac
     6841 agcacttctt atttaacaaa taccaacaca aacggatatg gtggagcaat tttaagtata
     6901 gctgctgcaa ctgcttctgg aactcaaaca gcgaccaata aaataaatat taatgaaaac
     6961 tcatttgacg gcaattatct ttcagtaaca aacagcagca ataccacaaa aggtgcaagc
     7021 agttcctacg gttatggtgg agttatttat agcagcctta cttccacggc ttcaggttca
     7081 gggattacaa ataccgaatt aagcattaat gacaattcct tattccgcaa taattccttg
     7141 aatgttacca atagtgcgat aacaacaaca ggatcaagca acgcatatgg ttatggtggg
     7201 gcgatttatg ctacttcttc cgtactcaac agcggaagtg gtagcgcaaa tcacctgacg
     7261 aatatcaaca acaatgtttt cgatactaat actcttagcg taataaacag tggaacaaca
     7321 ataacaggta caagcacggc cagaagttat ggcggtgctg tttttgtcaa tacaggccta
     7381 agcactaacc ttaaaaactc tgcttcaagc actacaaaca ttaaggataa cgagtttaaa
     7441 aacaattccg caactgccac aaacagcagt acaactacag gcacgagcaa cgcttacagc
     7501 tatggtgggg caatctatac aaacacgtat accggtaatt ctggggcggt ggctaattca
     7561 tctgtaaaca cgacaaatat cacgggtaat acctttgaac aaaacaatgc agttgcaaca
     7621 aacagcagca caacaatagg cacaaaaaca gccgctgatt cttatggtgg agcgatttat
     7681 aacactatgt cttcaacaaa ttccagtacc ggagttaatt caagcagcaa tactgttagc
     7741 attttggata atattttcaa gaaaaattct gcgaataacg gcggggcgat ttttaacacc
     7801 ttatcttcaa caaattccag taccggagtt aattcaagca caaatacctt ggatataaaa
     7861 gataacagat ttgaagaaaa tacggctact tatggcggag caatttataa tacctggact
     7921 tcaacaggaa caaacttgag tgtaaattct gtaaatatta cagataacgt tttcaaatta
     7981 aacacggctt cttatggcgg ggctattttc aatacagctg gtacattaac tgtaaacggc
     8041 aatacttttg atacaaattc agcaaaatac ggcggagcaa ttgctaatta tatatcaacg
     8101 acaaccggtt ccggaagtgc aactataaac gttgataacg gaacttttga aaaaaacttt
     8161 gcaaaaaatt cttcctcgac tgcagcgatt acttcatacg gtggggcgat ttttaattat
     8221 ggctatgcaa ctacaggaac aacaaacctt aacgccaatg ttaacaacag cacttttgat
     8281 acaaattacg ccaaagcaga atctacttgg gcttccacaa gcggatatgc aaatgcgcta
     8341 ggcggggcga tttataatta taaatcttca acaacaggtg caggaaattc tattttggat
     8401 gttgaaaata cagtatttaa aaacaactat acaaatgcat catcttcaag tggatatgca
     8461 aatgcccagg gtggagccat ttataatgca tcggccggga cacaagttgc aaaagtcact
     8521 atagacggcg gaacttttaa tgacaactat gttcaagcaa cggcaaaggg aaatgcatca
     8581 tcaacttatg cctacggtta cggtggggca atttacaact cgggtcaatt aaacgttaca
     8641 gacagttcat ttaacagaaa taatgccaat gccacaggaa ctacggcttc tacttacgca
     8701 tatggacggg gcggggcgat ttataacaca ggaacaacaa ccataactaa ttcgactttt
     8761 actgataaca ccgctaaaag tcagggtggg gcgatttata ataccggcac ccttaaatta
     8821 gtcgcggata acggtacaac ttcatttaca ggaaacaagg caaataacat ttctaacgca
     8881 ctatacttag ccggaggaac aacttatctg aatgccggca atggcggagt tatgacattt
     8941 aacgataaaa tagcatcttc tgccatcggc aatgttataa atataaacca aaccagcttg
     9001 ggacttgttt ctgggggcga aattattttt aatgaaaacg taagtgactc tacgattaat
     9061 ttatacagcg gaatgttaac cttgggaaat accggaaata cgagattaac cgattcaaca
     9121 ggacttaatt cgtctaattt ttacactaat aatgttaaat taaacattta tgatgccata
     9181 ttaaacctcg ccaacaataa tgcaacagat aggattaatc taaattcgtt tacttctaca
     9241 tctgattctt cgttgcaatt cgatgcggat tttgctacaa atcaaaacga tatgtttact
     9301 gtttcatcta taataaatcc ttccaatgtt ttaaatacag gactaataaa tattttaaat
     9361 gacggactta tatcatcatt gactttattt agtggcggaa tttcacctac cttgagtgct
     9421 tttagcactt ttactactga ttacaaatat tattttacac cttctacggc tggggtttac
     9481 gacgtaagaa gaacaactat agcaggctta aattatgcaa ttgaagacat tgctccaact
     9541 cgttcttttt cagccaccag agatacttat gttgactata atctaggtac aatgggtggt
     9601 gcagtctcca cacttgacat atttggcaac gataaaaata ttgtcggact aggatattca
     9661 ggagctaaca ctttggcagg tcaaacactg aatattacaa atgtcggatc tttaaaatcc
     9721 gacggcacag taaacacctc ttggaatggg ttttcttcaa caagcaacgg tggtggaatc
     9781 aacaataacg gaacagtaaa aattacagac agcgtatttt ataataacac ttcgactgca
     9841 aatggtggac tgctatataa tgcaaatgta gctactatta acacttcagc gtttgtgagc
     9901 aatagcgcag ccttaggcgg tgcaatttat aattcaggca caatgacagg attaaatgat
     9961 tctttcactt ctaactatac aacaactgcc aatggcggtg ctgtttataa ttcttctaca
    10021 gttaccttaa ctagttcgac atttaaaagt aataatgccc tgcttggaaa cggtggtgca
    10081 atatttaaca gcggaacatt taatttaaca ggtgctactt ttgggggttc ctccttgagc
    10141 gatgccaatt ctgctaaaaa cggaggagcc gtttataata caggaaacat tttcttgagc
    10201 ggctcattat ttaaaaataa ctcttcaagc gtaaatggcg gggcaattaa caatacagga
    10261 acaattcaaa taactaacag tatttttgaa aataacactg caacttctaa cggtggtgcg
    10321 atttataact cagggacttt aaaaataatt gctgatggag gaactacttc attcacaaac
    10381 aataaagcgt cgggagcttc aagcgccgtc tatcttgctt caggttctgt tatgaacgtt
    10441 aactcaggaa atggcggagc cgtaaccttt aatgacgaaa tcacgtccga gtccggtgca
    10501 agcattaata ttaacaaaat aaatgtttct acaaatcttg caggcacaac gtttgcccca
    10561 acagacggca aaattacatt taacgaaaat gttaataatt ctacaatcaa tttttacaac
    10621 ggaacaatgt ttttaaataa agaaaacctg ctagatggta acaatgtaaa cctatacggc
    10681 ggttctattg atttaataaa taaatcgact ggaacggcta gttttaataa tctttccatt
    10741 aacggtgtta ctaatctggc tatagacgct gatttagcaa acggcttggc ggataaaata
    10801 acatctgcaa accctatcac aggaaccgga agtctttatg ttaatcatgt aaacacaatg
    10861 ggtaattcgt cagctaaatc tgtaagtctg tctattgcag atgaaaacgt taaagattat
    10921 gttacggttt cacccggctg tgtaatgggg aatattttaa aatataactt gaattacgac
    10981 aaatcaacag gatcgttaat gtttttgtca ggtggctcaa gcggtgatta taaagcttat
    11041 aatcctgcaa tattaacctc ttctataaat atccaaacag gaagttactt aaatcaagcc
    11101 aatgtttata atgaagcttt gggaagaatg gatgcactaa tggcatcttc caaatacgat
    11161 agattattga tgaaatacca aaacaaagtt gcttctgccg atggtaattt tgtattttct
    11221 ccaaccttct tgcaggaatc aagcaaaggt ctgtggttta agcagtatgc gacgtttgaa
    11281 aatgtcccga tgaataatgg accgaatgtt tccaatgttt cttacgggat gttgattgga
    11341 ggagacaccg atatagtaag cttaaaacat ggttttgacg gatatttaac agcttatgta
    11401 ggatataacg gctcacacca aaattattat acaacagggg tttatcaaaa cgggggattg
    11461 gcaggattaa ccggaaccgc atataaagga aatttcttca cagccgtgac tttgaacttg
    11521 ggtggaaata acggaaacgc ctacacaaca gcaggggttg ataatttcac gacacttgtt
    11581 gcgggtgctg ctattaaatc aggttataat tttgaatttt tgcgagggaa gcttattgtt
    11641 caaccgagcc ttatggcagg ttacacgttt gctaatacct ttgattatac gacggcttct
    11701 ggagtcaata tgacatctga ccctttgcat gtgctacaac ttgcaccggg tattaaattc
    11761 ataggaaacc ttaataatgg ctggcagcct tacattgcag caaatatggt ttacaatata
    11821 tcaacaggag gaacattcgt tgctaacgat gtccaattgc ctggaatgtc aatagcgcca
    11881 tatgtagaat atggagttgg tatccagaga aaatggggcg acaggctgac agggtttgca
    11941 caaacaatgc tcagaggcgg tggaagaaac ggtatagctc ttcagtgggg tctgcgctgg
    12001 gcgttttaag catagcaata tctcaagcag taataaaata ataaaacaga tttgcatgtt
    12061 tacttaatac cataaataga agtttccaat ctccatctca aagatatttt tatatcattt
    12121 taagttgtta attacaactt ttatttaaac attatttatc aagtttaaaa atgtttttaa
    12181 tttcttcttt ttgttgatta aataaagttt tttgagtttg aatttgctgt tttttcttgt
    12241 ctattaattc tttttgagcc ttttgtcttg cctcagcctg ttttttttgt tcttctaact
    12301 gcttttgttt taattcttgc atttcttttt gtttttgttt tacctctttt tctttattta
    12361 caacaggtga tgcgacttta ttgattttct gattcaaaga atctacaagc cctccggtat
    12421 aagtttctgc agcaaaaact cttgtaccga tcaataaact taaaacacat gtaattgata
    12481 aaatcttttt catatttatg ccctcctata actcacggtc aaaattctaa aacaaataaa
    12541 attaaaaatc aaaccattaa aacttaatta aatttttcca ctctcctgct gcgtcttgta
    12601 attgtttttt agtatcatta acggtatttt taacttcatt tacagtagaa ttttttacat
    12661 ccgaatattg ttttttaact tcttctactg tatttttttt gacatcagta aactgttttt
    12721 ttacatctgt caaagtgctt ttaatatcta ttgcggaaat atctgtatcg gataaccatt
    12781 taaatgattt tacagaagac ttgctttcta tcccgccgtt aaaaacaact ttaaaatctt
    12841 tataatattt attcgagccc gttaagggag gtatttgtgc aacattttct tgggcgggac
    12901 ttgttgtcat agattttatt ataattgcgg taagtgtacc aaacttagga atataagacg
    12961 ttaatttatc taccgaaagt tctcctatag gacctaacaa tgcaactacg tctgagctta
    13021 atcttcctaa tacaacaaca tttgttgttc cgtttaacaa attatatctt ccgcttacaa
    13081 aataagacat tgtcgggccg cttgttttta tgtatgaaat atttgcccac ccgttattaa
    13141 atgtcatatc tccttttata tatttaaata atgccgtatt tttaaccgtt gatattgaag
    13201 caaccgtatc taaagctact ctcataacag agtttgcaag aatattttgg gcattcaaaa
    13261 agctttctaa acttccgact ctgccaagtg ccccttcttc aatcttaaat gaaaaattac
    13321 ctttaagcga tttgaggata tcggaatatt caatgcccct gaatttgatt ttagtatcaa
    13381 accccaatgt tccgctaagc gcatttttaa tccctgaagc ggctgcaata gcattagttg
    13441 cattcatatt ataaccttga aaatcaaccc ccgtttctcc gttaataata ttgtaaacta
    13501 tatcaccttt aagcttaccc ccgtaagcat cgcctgtaat attttttaaa taaaataaat
    13561 ctccttttaa tataaaatca gaacttaaat tttgtgcaac aagcccgctt gatcttattc
    13621 tcttaactgt accgttacct ttgacaatag gtccgtcaag gtttataacc atatcatcca
    13681 ttgaaaccaa catatcggga atataagtgt atggaataat aaagactcct tttaatttgg
    13741 ggttaatcat agatcctgag attacaacat ctccttttgc ctgaatcttt gaatttgcaa
    13801 agccgggaat tgaaaaattg caaagtcttg gaattgaata atttaagttt aatttttgtg
    13861 agctgctgtt aacagttcct tttagtgttg attttatatc cccgccggtt ttgatatcca
    13921 aaacaatttt atcggttaaa taatttttaa ttaagcccga aacattcaaa tttattttat
    13981 ctataaaaac agttgaagga cttataataa cttcttcagg ttcaatattt gcaacaagct
    14041 tgggcacata aattactgcc gcaggattta taatcttaaa atctacggaa tctgcggtcc
    14101 ctttatatgc ttttttatca gattttagtt ttatattaag cgccgacaaa atcaccgaag
    14161 aatttgtcgg aatatttttt aaattcactt tatttacata gagattaact gctgtttcag
    14221 gctttgttaa ttccccgctt aatgaagcct ttaagcttaa atccccgctg ttaaatttat
    14281 tttcttttaa taaactcacc tgccctaaag aaagcaaaag actttttatt gctaattttt
    14341 cagcaacaat atttatatct gcttttgcat cttgataaat tgttccataa atcctaagcg
    14401 gatttccaag aattgaaaaa cctgtttttt taatgtttac aatattatta tcaaaactta
    14461 tgtcagaatt aatatcattg atggcaacat tatacaaacc gtattttaaa cttccttgag
    14521 gaatcttaaa atatcctgag gagagaattt tttttaggtt cgatttaatt ctaaaatctg
    14581 catttatata accgccggca ctgagagtct gaaaatcatt aatatcaaaa gatgccgtaa
    14641 tatttttaaa tatttcaaca agattattca aactcaaatt tgatttaaaa ctcatatcta
    14701 tatccggctt tttacctgtt ttaaaaacac ctttaatgtg tgtaatttct ttttctgcag
    14761 tataaaaatc gcaatcaaga tctattttat tacccttgag ttttaataaa ataaagctgt
    14821 cgggcagttt tttattatta atcgcaagac ttagtttatc aatatttata aacccgtcaa
    14881 cttttacatc ttctatgtca ccgtaagttt ttacatcaac ctttatattt gaattaaatt
    14941 tattactgta aagtgcttca aaaatatcaa taatattaaa aataaattgc tgaggctttt
    15001 gttctaaggg ctgggggtta aaaatcaaat cgtttaaatg agatttaggc ataattttat
    15061 ttaagatttt tatgtcataa tcaagttgtt ttctttcatc aagaatcatt tcacccttgc
    15121 ctgaaatttt tatttttttg ttaaaaacaa aatcggtaat ttttgtatca catccggata
    15181 aagaatattt tttgcccgac aaagcctcaa caaatgtaat tttatgagca tttattttta
    15241 catccggaag attatcggat aatttaaaag gcaaagattt taattcttta ttttcttctt
    15301 tgtttttagg aatataattt tcaattaaaa attttccttt tttttgaact tttaaaacaa
    15361 catcaatgtt atctgcgctt ataaggtcca actctatttt tttaaataaa acaggcaaaa
    15421 gtgaaacctt tatttgaaaa ttatccgcct gcaaaaattt ttctttattt ggcaaaaata
    15481 tttctgtttt gcccgctttt atgcctaaag tgaattttgg cgtagttatt attcttattc
    15541 tttcaagatt aaccttaaag cctgattctt tttcaataaa ctctgaaatg tcagatttgt
    15601 aactatctgc aatcggagaa agaattaaag gcaaaattaa aaataaaaca taaattgaac
    15661 aaaataaaac cgataaaatt tttgctgtca aaataagatt ttttttcatt ttttgccccc
    15721 ataaaattaa gtttataaat actatattaa ataaattatt ttttcaataa tcaataacgg
    15781 ctgccgattg aatttgttgc cgaatcgaat gaaaaaatca aatccagata acttttgttg
    15841 tatttgacag gaagcggatt aagcggcata cattttctta ctgcatcaag tgccgcgtta
    15901 tcaacagccc ctatttcgga ggttcttttg acgtttaata tcataagcct tccgtctttt
    15961 gctatgcgca aaaacaactc cactcttttg ccgccggctg taggaggaat tttccaactc
    16021 cacctgactt ctcttttcaa ttcgtttacg taaggcgaaa tatcataagc cacgattaaa
    16081 ttttttgtag caaccttatt gaatcctcct ccgcctgtgg aagaagaatc cgaagaagac
    16141 gagccgcctg ctgctgatac agaatttgtc gaaacctcta atcctgaagc gtggattctt
    16201 gaagtttttg aagaacttaa tccggaactc attgatttaa aatcagggat aggaatctca
    16261 aaatccgaaa ctccgcttga ttttattttt tgtgtgacag attttgtctg tggcattgaa
    16321 gaagttttta acttatttgc aggctcggaa gaaacttgat tttgattgtt ttgagataca
    16381 gagcctttgt ttgcaggacc tgcaagagta aaggctatat cttttacttt catcacattg
    16441 tctttaattt ttggagtaat ccctaaaaaa ttcaaaagaa aaacaaaaaa acaaattaaa
    16501 ataatacctg cgatatgccc gaaaaccgat ttgaacaaaa aattatacaa agaatcctca
    16561 agcggcgatt tcctgactgt tgcaggaacc ccgtcccaaa aaattttagt gtgacccttt
    16621 tttctatttt tttcttgcgt tttcaagcag agttaacctt tttttaattt gtattgcgct
    16681 ttcatcttta ggacttagtg ttataaattt tttgtagtat aaaatcgcat cgggataatt
    16741 tttttcttta tcgcaaatca ttccaagcaa atagtaagca tttgaaaaat ccggttttgc
    16801 tcctgtagcg cgaataagat attcttttgc ttttttgcta tcacctgatt caaagcaaat
    16861 tgcgccaata taaaaaagtg catctaaatt ttgcgaatca accgtcaaaa cctgctcata
    16921 atatttttgc gcctcagtcg gttttgaatc atttttataa acatttgcaa ggcttaacat
    16981 taagtcgaca ttgtttttat caagatccaa tgcctgtttg taagcctcaa tagcctcttt
    17041 agtactacct tgaagcaaaa gcgcatcagc caaatcacaa tacaaagtag cgtctgtttt
    17101 attcaaatta accgcctctt taaaatttgc caccgcactt acatagtcat ttttttgatt
    17161 gtaattgttt gcaacggtca catataattt tgaaagttct gacttgactt tagcatcatc
    17221 ctgaagcgaa atcgattttt tgtagttttc aactgctttg tcatactctt tcatcaaggt
    17281 ataagaaagt gccatataat aatatatttg ggcattgtcg ccggagagtt taagtgcttc
    17341 attataataa tttatggact gcacaaaatc gcccgacata taataatttt tgccgatttc
    17401 ttttaagatt tttacatctt ctttttcttt acttttaagc ttattcagag ttgaaagcga
    17461 attgttaaac tcttgttttc tttgataaat atttgcaaga ttgtaataag cctgagaatt
    17521 atcgggcagg gtatcaaggc atgcttcata atattttatg gcatctgctt ctcttcccag
    17581 ttcatctgaa agttttgcga tgttgaattg aacttccaca agagaagggt ccattttaaa
    17641 agcactttga tattccacaa tcgccttttc tttttcgcct gtgttttcgt aagccgtcgc
    17701 aaggattatt tttaattgaa cattttcagg atcatcctgc attgcttttt gtacaacttc
    17761 aggaattttt ttaaaatttt cggtcaacaa aagtgctttg gcgtaatttg tccaatcgtc
    17821 aaaatcatca ggggattttg ccaaaatcgc ttcgtattca ccgactgctt cggtgtccat
    17881 atcgaggttc aaataaagtg cggcgaggtt ttttcttgca tcaatgttat caggatatga
    17941 gataagaaat tttttgtact cttctatcga ttctttttgc tgattcatta attcctgcat
    18001 ttgagctaga tttaaatgaa tgtaagtgta atttggaagc agttgggaag ctttttggta
    18061 tgcttcgtaa gcccctttat aatctttttg tgcgcgcaaa acagctgcaa tatttagcat
    18121 tatagaaccg tcatcggggt ttaatttgga agctttttgg taatacaaaa gcgcgttgtt
    18181 ccagtcttgg atattagaat ataaagtcgc aagttttaaa cagatactta attcatcggg
    18241 tgccaattct tctgctgctt tgtattctat ttcagcatta tctgtatcgt ttattgcaaa
    18301 atattcgttt gctttaatga agtgtttata agctttttta ggcatggcaa aagcgacgct
    18361 cccgcagagg aaaactccca aagtatatat taaaattttt ttccccatct ctttcataaa
    18421 agtacttgcc aataaatttt ccgaatgtat aattaaatag tgaattaatt acatttatat
    18481 attaatagat tagcaaattt aattcaagta tagagatagt tgaagttatg aaaaaggaga
    18541 agaaaaatga gactagcgat tagaaatttg gttttgtcct tatttgtaat cgggctgatg
    18601 gcaagcaatg caattgcaaa aagttatccc gatttgcctg aaaatcactg ggcatacaaa
    18661 caaatacagg cacttacgga tgaagatgtt cttgtgggat accctgacgg aaattttaaa
    18721 cctgacgaaa cagcaacaag agcagaattt accgcaatgg taattaaagc acttcaccag
    18781 gacaaagcac ctttgaaaaa aacatttgat ttcacagatg ttccctacaa acattgggct
    18841 tacaatgtta ttcaaagagc aatcaatttt gatttaatca aaaacacatc tgataacacc
    18901 ttcagacctg aagataccgt tacaaaaaaa gaagcaatgg atataatggt atctgcgctt
    18961 aacttagaca aatggagcct tttcagagcg caaaaagcaa tcggaacatt caaaaaccct
    19021 gatgctccaa taacaagggc agaaatggct ttcgggttat acaacatgca aattaacgca
    19081 agaattaacc ctaacaaaaa gcttcaggaa gctatgagag caaaaagagg cgaaggttat
    19141 gttctaagag gcgtaacaat cgacggaaca gttgccacaa ttcctgcagg aacaacaatt
    19201 cctgtaatgt tacttacacc attaaacagt tctgtcaata aaaaaggcga atgctttgta
    19261 acaaaagcaa acaaacacct tgttacaaaa gaccgttata ttatatttgc aagagcaagc
    19321 aaaattaacg gtgaaataac aagcgtaacc cctggcaaat tgtttgtaag aacaggcaaa
    19381 atgggacttg aaacaaaaac catttataca cctatcgaac aaattgcatg tttccccggt
    19441 tgcattaaca cagaccagca aagaaactgg tttatgagaa taatcagagc aattatcaaa
    19501 ggcggaaaag tgaaacttaa agcaggtaag gtggtcacaa taacaacact taaaaccatt
    19561 aaagttgatc ttacaagcgg ctggataatt gaataattaa ttataataaa aagagcaaag
    19621 cctcgggaaa tcccgaggct ttgttttaat attttatgtc aacaaagaaa ttatttgagc
    19681 atatttttct tttttctgct cctgttccct ttggtaaatt tcttcttcag agagttttct
    19741 ccaaacagaa ggtaaataaa cctttgggct taaataatca ttcaccgtct tttcgtcctg
    19801 ctcataaaaa ataggctcaa aacctgcttc tcttaattcc tgatgaacaa aagaagtgtc
    19861 ataaaccgtc aattctttac cgtcttgatt aattttatga aaataatccg ctgtaacagc
    19921 cctcccaaat aaatgatcag cattcaaagc acccatgaca aaaatgcctt tatcagacaa
    19981 cattgaatat attttactaa aaagttcttt gaccggtttt atgtcaaaat caaccaaata
    20041 tttatcgtct tttatttgat acatcaaatg gtaaagggca tttttaaaaa acactacgcc
    20101 tgttttattt ggagttaatt ccttatcaat atttacaata tctgccaatt taaaatcaat
    20161 aacgtcatca aaaacgcctt tttttggaac aaaataatcc cccgctttta caaaattatt
    20221 ttgtgtttga ataaatttga ctcttgccag atcaatttct gcatcccctg atgcgcaggg
    20281 taattttgta actttctttt ttgctcttgg atgttgaagg ttaaactttt cccaatccca
    20341 accaagcttt tgaaaacatt cattaaaatt gcttttaatc tttaaccaat tctcattatc
    20401 ttggcctaat ctagaaacat aacggtcctt tgttaaaact cttgcaacaa ccccgtcgta
    20461 attgtaagaa agactcaaaa atcctttttc tgcatcctct attgcttttg ttgaaatatc
    20521 atagcctgta atcttatatt tcttatcctt attgcaatca tgaagcaaaa tgcccaagct
    20581 gtaagtttca tcaccgtttg aacatgcaaa atctgctata tttgcgcctt tgggaaattc
    20641 ttttttcaga taatcacaaa caaaacccaa actatccaaa ttcctcataa aacttgaatc
    20701 atttatacaa tcatccgctt tattattact ttcaacttgt tttactgtgc ctcgataacc
    20761 acgaaataaa atatttgaag tattttgtat ttttacaata ttcataacag tattgtaaaa
    20821 accgaaaaaa atttttttgc cgttttatta atttttattt acaaactcac cctgcagata
    20881 ccatgttttt gcgcctgtga tttttgcatt gacaaattgc ccgcttaaat ctttttcaaa
    20941 aggaatatga acgattttgt tattccttgt tcttcccgtt attatatttt tgcctttatg
    21001 gttttcaaat ttttcaatta aaacctccat ttcacgcccg ataaattttt tgtttgaagc
    21061 aagtgcgctt tcttttacct gattgttaag ccttgcaagc ctttgatctt tgacttcttc
    21121 ggttaaaaat aaattcttcc attttgcggc aaccgtctgc tcgcggggag aataagccgc
    21181 agtattacag taatcaagct ctaattcttt tattgcagat aaggtttgtt caaactgttc
    21241 ttcgctttcg cccggaaaac ccgctataaa atcgcttgtt atggtaacat cttttacctt
    21301 atctctaacc ttttttacta tttcaaaata ttttttcctg tcatatcttc tgttcatttt
    21361 tttcaaaatc tcacttgagc ctgattgcat cgggatatga aaatattcgc aaattttgtc
    21421 gcacttgatt acagtttcta tcaactcatc tgtaatatct gtaggataag atgtcacaaa
    21481 acgaatcctg aaagttcctt caattttgtt aatatctttt aaaagatgcg ccaaaaaata
    21541 cccctcagtg cctttgtagc tatccacatt ttgccccaaa agcgtaattt ccttgaaacc
    21601 ctgcgaaagt gcttctttga cctctttgat aataacctca ggtgctctcg accgctcgcg
    21661 cccccttgtg taaggcacaa tacagtaagt acaaaaatta tcgcagcctt cggttattgt
    21721 aatccaagca ttaacaccct ttgccctttt tagtttaata tctttttcag gcaaaggagt
    21781 ttcgtctgtg gcacaaactt tttcacccgc ctcaatacgt ttaatcaaat caggaaattc
    21841 gttaatgttg tgtgttccca taattaaatc tatatagggc atccttttga atattttttc
    21901 gcccccttgt tgggcgacac aaccacaaaa ggctattttt aaatttttgc gtgttttttt
    21961 ccatttaccc caaagcccta ttctgctaaa agccttgtct tctgagagct gtcttattga
    22021 acaggtattg attataagca ggtcggcttt tttaggctct ttaatttctt cataacccat
    22081 aaaagaaagc atcccgagca tcttttcagt gtcggattta ttcatctggc agcccagtgt
    22141 ttctacataa acctttttca tttcttaatt atataataaa taattcaagt atcaaaaggg
    22201 ctggggtttt ttacaaaaac cccagccctt ttgatttatt aatttattta ctcttcgtca
    22261 agtgcaaatt cgggagtacc gaacattcct tcgatttctt ctaaaatcgc agaaggtttt
    22321 cttgcctgat ttcttgccgc tgctcttctt tgagcctctt tgtctttttc ttcgctggca
    22381 tgagtcaagt ggtgataacc tgttcctgca ggaattaaac gtcctatgat aacgttttct
    22441 tttaagcctc tgagcaaatc ttgttttcct tcaacggcag cttctgtaag gattcttgtt
    22501 gtttcctgga atgatgctgc tgaaatccag ctttctgtgt tcaaacttgc ttttgtaata
    22561 cccaagagca tatattcgca agtggcttca actccgcctg cttcttttgt ctctttgttg
    22621 gcattttcaa atgtttgaac ttcaatcagt tcgcccgcaa gaagttttgt gtcacccggc
    22681 tcgataactt taactttttt agtcatctgc cttactataa tctcaacgtg tttgtctgcg
    22741 atttcaacac cctgagagcg atatacacgc tgaacttcat caaccaaata ctgttgtgta
    22801 acttcaggac cgtttaacct cataacatca tgaggattca aagggccgct tgttaaaggt
    22861 tgaccttttt tgattttttg tttatcggca acgataatat ttgaatctat tgtatgttta
    22921 atttcaactc tgccgttttc atttaccaaa tataaagtag gtgtatcatc ttctatagcg
    22981 atttctgcaa caccgtcatc ttcggccaaa atagaacatt ctttgggttt tcttccttca
    23041 agaagttctt caacccttgg aagaccctgt ac
//
LOCUS       DAOQAU010000025        22626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2310_length_22626_cov_5.844978, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000025 DAOQAU010000000
VERSION     DAOQAU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 22626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22626
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_22626_cov_5.844978"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKI94_05015"
     CDS             <1..429
                     /locus_tag="PKI94_05015"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNW26135.1"
                     /translation="TIALIIGHLTVAKHCFKKPVTLEYPEKQPVLNEHFRGEHALVSD
                     ETGKLLCTACGTCQRVCPSFGTIEIEKEKNAEGKFYPKNYSIDLNKCIFCGNCVQYCP
                     FGAIIMTKKFDLANEKKSSLKLDINTLKKNYSDNIIKKGL"
     gene            522..1070
                     /locus_tag="PKI94_05020"
     CDS             522..1070
                     /locus_tag="PKI94_05020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNW26136.1"
                     /translation="MEIDILKSLIFYGFAGLILLFAFLSIFSFRILYSLICAVCVFFA
                     TAGMFFLLGADYNAVIQIAIYGIAIPILFIFAIMFTSDKLDKSVYITFKPRFYFAFMS
                     AGVLFLALIYLLATSLGLSSHSNWILQKQTMFINKFQMFKAIADGFFTNYVVAFELFS
                     ILLLILVVGFSTFNILKEKNDD"
     gene            1063..1398
                     /gene="nuoK"
                     /locus_tag="PKI94_05025"
     CDS             1063..1398
                     /gene="nuoK"
                     /locus_tag="PKI94_05025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNW26137.1"
                     /translation="MTDILELIFNIGLYHYLLLALILFIMGFFGAIVSKNVLKVLISI
                     EFMLTAVNINFIAFASFFDNIKLEGFVFSLFYIALGAVEVAIALSIFYLMFRQKRSVN
                     TEDYKELKG"
     gene            1416..3326
                     /gene="nuoL"
                     /locus_tag="PKI94_05030"
     CDS             1416..3326
                     /gene="nuoL"
                     /locus_tag="PKI94_05030"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNW26138.1"
                     /translation="MDFFFENIYTIMLFPIWIFLIISIGKFFGILDSKPAISGLTLIS
                     TLWGLIFSIGILLGILHNPNFSYESSITFLKIEDYALNLGVYVDNIAAVMLILVTGIS
                     FIVQLYSSVYMKHDASYPRFFAYLNLFNFSMLGLVLSSNLFQTYVFWELVGVSSYLLI
                     GFWYKKPSASKAAKKAFIMNRIGDFGLLAGVISLSYFMYQYSGFPQLATIPFASISDI
                     ADYLFSYTNDFVFITISIFMLMGAIAKSAQFPLHTWLADAMEGPTPVSALIHSATMVA
                     AGVFLVARLYPIFVLNHYVMDFIAIVGLITALICAYFAITQTDFKRILAYSTNSQLGL
                     MFLALGAGAFTGGLFHLVTHAFFKSMLFLCSGIVIHSVQSHQDVRFMGGMRKVLPITA
                     LCYLIGCVAISGLCFSGFYSKELIINAMLSSKNFIYVQAFLFVSLMTAFYMFRSYFLV
                     FEGKYRGSHEPEKQGGILNISILILAIFTVFLGYFTPFFEKIIPQMAQLPQIKWAVSP
                     AFVSISIGITGFLFAWIFYNSRVNAFKLPLLYKLSYNRFYIDDLYGFIADKLYYWTSR
                     ICSIIDKYLVDGVVILSALCTRAFSWTFSKMQTGNFQSYLAYTFAFLAFAFTVLMFAY
                     TAILSFGLLSGY"
     gene            3339..4859
                     /locus_tag="PKI94_05035"
     CDS             3339..4859
                     /locus_tag="PKI94_05035"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNW26139.1"
                     /translation="MNFLSVLVLIFSPLVASGLIFTSFFKNNEVRIRRFSKTFAGLHF
                     LYTLMFLVFFNPQAEGISYYKEITFFGSGWIEPLGLKLAFGLDGLALLMVILTSLIVF
                     LALIASKSNIKQKHKFYYSLIFLLQTAVLGVFCARDMFVFFMFWELELIPMYFLIGQW
                     GDAKAKSSAMKFVLYTFFGSLFMLLGILLLHFYYFATYGVMTGDFSSILVSAPEFPLA
                     LQVLISILFLIGFAVKLPLVPLHTWLPDAHVDAPTPVSMILAAVLLKMGAFGIIRFNM
                     QMLPDAFKVLVPALLIMALINIIYTAFIAYSQTDLKRVVAYSSISHMGIVLFGLCSLN
                     LMSFSGAVFQMLSHGLISAGLFMVVGIIYIRTKTRQISELGGLAAVMPRLAGFSIILT
                     LAAVGLPLLMGFVGEFMVFLGSFNSALDDILPIKLYTLIAVLILVLSVCYMLRLLHET
                     FYSNLLEKWHNLKDIMMHEFTVLSIISFCIVVFGVFPMFIINIVQPLLTGILEFLR"
     gene            4870..6312
                     /locus_tag="PKI94_05040"
     CDS             4870..6312
                     /locus_tag="PKI94_05040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNW26140.1"
                     /translation="MEFFNNILNTMLPEIAILLFIFILIILSMTLDLKHYKLAKWITL
                     LGIFTATALCTKIQVEPIAYGFKDTFISDYYTLFFKFVLLIASFIIVFLTKKAITSRR
                     DNAFQFNAILLTAILGALILVSANDFLTMFISMETLGFATFFLLAFDKGYKSKEATFK
                     YILISAVATAVFLFGVSYIYGLTGTVNFSKIYEYFLDVDPTLLYSMATIFIASGLCFK
                     LGVIPFANWILDVYEGADTSVVTFVSIIPKVAMFSILSRLLVFAMSYSFELTFTLVVI
                     SVMTALWANTLAIRQTNIKRLLACSSSANAAYVLFAASLVSVFNLSTVLFYLVAYVFM
                     NIGVFSAVIILENSQFSNRLYEFKNFAYTNPIFTLCFSICILGLAGFPITSGFVSKLY
                     LFSAIIRSGLVFLPFLIIMMLTIVASVFYYLTVVRLMFDKSDKVESEVIPHKTSSAIV
                     ILYFCALITVIMGIFPSKVIELCQFIAYNV"
     gene            complement(6315..6632)
                     /locus_tag="PKI94_05045"
     CDS             complement(6315..6632)
                     /locus_tag="PKI94_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26141.1"
                     /translation="MKKFLSLFCVLILSSPAFADLSVNDISSQKYIDNHGHSDEMSRL
                     IDLQKSQINEVPTEYKSAQPEIYLTPVSRALRKVFMYLDCGLDDGLFMQHNIDYTTRY
                     DDI"
     gene            complement(6714..7769)
                     /gene="ispG"
                     /locus_tag="PKI94_05050"
                     /gene_synonym="gcpE"
     CDS             complement(6714..7769)
                     /gene="ispG"
                     /locus_tag="PKI94_05050"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNW26142.1"
                     /translation="MINRRKSKQILIGGVKIGGNAPITVQSMCNTDTRDIKATLAQIE
                     SLTEAGCELVRLAVLDKVAASAIKDIVKKSQIPVIADIHFDYRLAVECINKGIHALRL
                     NPGNIGNLENIKKVVTLAKQQKIPIRIGINAGSLEKEIAKENISLPQKMVKSALKHIK
                     ILEDLNFDLIKISLKSSDVLSTIEAYKLIADKVNYPLHLGVTEAGTLKSGLVKSSVGL
                     GSLLSQGIGDTIRVSLTENPVEEVWAGFEILKSLNLRQKGVNFVSCPTCGRCQIDLIN
                     LAKQVEEKFKSLKEPVTIAVMGCPVNGPGEAKHADFGVAGAIGEGYIFKKGEIICKVP
                     ESEIIQKLDEIIKKSLK"
     gene            7845..8588
                     /locus_tag="PKI94_05055"
     CDS             7845..8588
                     /locus_tag="PKI94_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNW26143.1"
                     /translation="MKVAIISDIHGNMQALESVLADIKAEKCDKIFCLGDLAMAGPEP
                     VKAVKLIKKMAEDENFYLIQGNTDEFIANYSPELKEKIASNFPVMAAALENDVKIMPE
                     KDIKFLKNLPKQLELKVEETKILLVHGSPRNNGENIFPDLPIEKVEEMLKGVDADLIF
                     CGHTHIPCGYQTNTEQTVVNDGSVGRPFTSEPKACYVIAEFKDKKFNIMHKFIDYDKQ
                     KASEILAKRPFKGSEKLAQMLINPELRHI"
     gene            8638..10014
                     /locus_tag="PKI94_05060"
     CDS             8638..10014
                     /locus_tag="PKI94_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HNW26144.1"
                     /translation="MYELYPYFTNDGSVGLFSRQDDDIYHSAYGALSESWQKFILPAH
                     LQEYLLKHDSVKILDICYGIGYNTKTALQTFLNVSEKKYKNNLKNKKVFSKNFSHPHT
                     SIAAIDTDNIYREDFEQIYTDTPLYNAEIYSDNISSENKTVSEEKISKIFIDAVDTDK
                     ILMQVSPFITNENKNYFFCNKNNFYFEKNCENKFEKIKKIKKNKKLKLEKKYKIKNEV
                     LILIFQKLIQNNPEFFDKILTSILYQKKISPFLSKFMLNFARFYQNQGYNNNKNLNNS
                     TFLHNIYYRYISKSYKNAKKYDKNNKITLNFHHFDARKYVKTTKNTYDFIFLDAFTPS
                     KCPCLWALEFFEQLYSKLEDDGMILTYSNSAAVRNAFLKSGFFVGKTFDSKLNKFIGT
                     VASKNPDLIEHKLNEQDLELMKSKAGICYRDENLAFDNHVILANRDMEIERSELKSSS
                     QVLKGTNQ"
     gene            10011..11282
                     /locus_tag="PKI94_05065"
     CDS             10011..11282
                     /locus_tag="PKI94_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW26145.1"
                     /translation="MTSDKYDVIIAGGGTAGCAAAYTCANLGLKTLLIEKNIHLGGTM
                     TSGLVIPAMNSSKNQINTDFFNALISELADLKGQITYENNSGWFNPELCKIALDRLMS
                     KSGVEVLFDTRIIGVNAINRKINGVTISSNMLSVYIATSNVIDATGNCEIGKILGCNF
                     LENKNEFQPVSLRFQMSGIDLDIFGKWLLDFDKDKTVTSMHVIDGQVHLSTAYTWDKD
                     KNWALKPIFEDAVTKNVLKDTDRSYFQVFSIPNMPNSLAFNCPRVYFDKEINPLDNVN
                     ISKALIVGRESIIRIADFCKIYFPGFEKSYISNIADALGVRVSRRIKGKYVYTVDDLK
                     SGKKFKNPVLISNYPIDVHSNKDGKSILEKTEKEYQLPVEALISADYDNFFVAGRCLS
                     ADFFAQAALRIQPSCFSMGEGLAKHIASVVN"
     gene            11330..11908
                     /locus_tag="PKI94_05070"
     CDS             11330..11908
                     /locus_tag="PKI94_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNW26146.1"
                     /translation="MGDKKLDGTRTQQCLINAFAGESQARNRYTYFASVAKKAGYEQI
                     CALFLETADNEKEHAKLFYKYLGSGVFNVHADYPVHLGTSLENLKHAAAGEHDEWSNL
                     YLNASKIAQEEGFYDVAETFKHVCIVEKHHEARFLELAKNIEENTVFKKLSEVNWKCR
                     NCGFIIKGADAPLICPTCFHPQAYFELLCDNF"
     gene            12087..16100
                     /locus_tag="PKI94_05075"
     CDS             12087..16100
                     /locus_tag="PKI94_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNW26147.1"
                     /translation="MLENIVNKELGIQIKFKNPQLKMGLVPSVWINADEFNVLDANTS
                     PLTVKNPKLKIRLFPLVIGKIQPSYFSCDKLDLNIKVDKHLRLYLGNILFIEKANPKL
                     SFEDMQTYIQEYNIFLNDEFQGKNILFSGDYFNLYNFNSKKNIKLSTNSKIKVNNKTS
                     VIDIDLDFKLPFKKSFETKDVVFDGTVTNLDLGIFAPYIKKFTKERILNTQGLINIET
                     KTKILDSSKKRINSLISIQGLKIIEKDKPSSIIFDKKLDLTAILDVSRGLLNINKFKI
                     KSDKIDTEIFGEIQRINAQNPTLNLNIIVEKSRIEDLISLIPTDFNIKEVNLYALKKY
                     GYYSDIQGNLSIRGRADRPNLKGKIISTNAYVIKPLPLHIPKAIVSLDFLGEKFFLDV
                     FVPCEKAEHVSVKGDIYLYDDKKTYLDITSTKNVNLKTAQIVLNPIHEIFQFDLGPLP
                     VMDLHGFGNINLKTAGTKANPSLFGVFNFRDASVKINGLEMLLTNANGKLEFKNANTY
                     FETSQAYLNNKPAKVKGTCTLSGELDYSAVANGQDLGDLIYILKNSPILGDMQKQVSQ
                     LQNGKGKIDFTLKLSGKVKSINDFKLGKNVFVQGTIKLFGNNASIQGLSLQVKSILGT
                     IKYKNTDLEVDLLSNLNKSKVKIEGKIDDNNAKLNFKSDKLYISDIIDVLPSLSFDNF
                     KYISTNSNNYINLKASYFGPVNKIDTDKISIYGKTKINNVKFTYLPQNLPVNLYSGVF
                     EIKGNTINLYKINTTLDSVPLLVDGTIYNIFKNPNFNIYVNSRPNQTFIDKYINTKAI
                     YPVKIKGDITFSSKIQGTKKLFSTKSEINLQDGANIYYMGSALGDSQTPIKIFLNADI
                     APTYININNFQYDKLISSQNGKEFVQNQLSAKGLVDFNRSKKILLENFYVKTSNPTDA
                     KIFNMIFRKPLIKQGQFTSDIAINGDLSRPRMIGDLDFTGIDIPLLDTTVKDISLDFF
                     NEYISIKAIGEIFSNKILLESTMKNKLEVPYTFNEINVTTDKLDINAIAKSISNLQIE
                     SDKHKITNENQSIDLSNLIINNAQLKATNVLVKNVQASNLIAKMSLDEKMLFSLDEFS
                     FDLGEGKVKGNFNYNLLNSKSKLNLNFLNVNANQVAESLFDLSNQIYGKLKGEAEIMC
                     NAKSEKLCMETISGQGWFEVANGKMPKLGSLEYLLRASNVVKSGITGLSINNIIDLLT
                     PLKTGHFDNINGNFDIDSGFAKTIQIYSKGKDLSLYLTGTYNFSTLIADMNIFGKLSK
                     KISTVLGPVGNASLNSVFNAIPGISIDEAKQSKLYSEMIKIPDFELNDELYRIFTVKI
                     YGDINGENYVQSFKWVE"
     gene            16102..16869
                     /locus_tag="PKI94_05080"
     CDS             16102..16869
                     /locus_tag="PKI94_05080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNW26148.1"
                     /translation="MENFIIDTHSHIDMQDFENINEIIQNAKNSGVNKIIVPSVDRSS
                     FKNVLELAQSYENVYCALGIHPSEAQNAADKDFEEILKLGKEKKIVAIGECGLDYYWS
                     KDFIDQQKIIFIKQIDIANQLKKTLIVHDRDAHKDTFDILTANIKDINVVMHCFSGSY
                     EFAKECVKKDFYIGIGGVVTFKNAKKLHEIAKNIPLDYILLETDAPYLTPVPFRGKRN
                     EPAYTKFVAEKIAEIRGISTDDVIKITTSNARKVFGI"
     gene            16866..17666
                     /locus_tag="PKI94_05085"
     CDS             16866..17666
                     /locus_tag="PKI94_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNW26149.1"
                     /translation="MKKVRLDNFLVNLGYFDNKSKASANIMAGNVQVDEVIITKAGHH
                     LDTAKNYEIKVKTTPFVSRGGLKLQKAVETFNVDIKNRICLDAGASTGGFTDCLLQNG
                     AQKIYAVDVGYGQIDWKLRSNDKVKVIERTNIKNCDFSQIYLPKEPIADLAVMDLSFI
                     SLKKVLPNIKTLLKENHEIIALIKPQFEVGKEHIAKGGVVKEKNAQARAIYDVIDFAK
                     TLGYNAIDLTYSPLKGPAGNCEYLIHLSTETAKQQDFLIKNIVNEANT"
     gene            17663..19045
                     /locus_tag="PKI94_05090"
     CDS             17663..19045
                     /locus_tag="PKI94_05090"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNW26150.1"
                     /translation="MNTKLIEKAVYELCIQANTQLCDDVFAKICNACANETNKNSKQA
                     LELILQNAKMSFETKKPLCQDTGQVLVFLKIGEDVKIKNLTKSINNAVFKAYKQNFYR
                     KSIVKNALFERINTNTNIPCIIYTQIVKGKGLEIELLIKGAGAENASAAKMLSPTANE
                     KEIIDFVIDTVKSAGAKACPPFFIGIGIGGTIEYSGLLSKKALLLDKNIDENHEKLAQ
                     KIQNKINNLNIGTMGLGGNFTALDVKILTSSTHIASMPITVTVNCHSSRHAKCKIENN
                     KIKFYNSKKIRNYKPFENKFNDYIKLNTNEINKIRKLKTGQKVLLTGELYTARDMAHK
                     MLVELIQKGKKLPVELKDKIIFYAGPCPNPKSEIIGSVGPTTSARMDKFMPEISKTEI
                     LATIGKGERSAEAIKLMQKNKIINFGAIGGIACLLSKKITKKEIIAFEDLGTEAIYRL
                     CVHDFPVYVS"
     gene            complement(18986..19810)
                     /locus_tag="PKI94_05095"
     CDS             complement(18986..19810)
                     /locus_tag="PKI94_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW26151.1"
                     /translation="MTGLDVNSKLSLNTIHNTAFITVPKYKSSKISFASNFDDSFSFN
                     NEIQQYLTPMAVNRMLSLNPKISEVLKSNNLPVKINTDELNNLVKTHLSKTRETLLGI
                     YQHLDDKYRHAIDFESVQKASMLHDIGKVLIPAKLLNKPQALSLNEFNIIKLHSELGY
                     QMLKTTDLSPKVLDLVRYHHQNPFNTGYPKRTNSEVVDLNLQLLSVADSFSALIEKRP
                     YKKALRINKALEIIHKKMKKGEVHPYLFKALVDYANESRNINGKIMDTEPVNRLSA"
     gene            complement(19908..20867)
                     /locus_tag="PKI94_05100"
     CDS             complement(19908..20867)
                     /locus_tag="PKI94_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26152.1"
                     /translation="MSKISLIEKITDIAEKILYKNKGFEFYYNRGIDYFNKKKYEKAL
                     LEFKTALECQHPQPQVYYNMGLTHHYLGNYEKAVTSYNKFLESKPEDYDGLYNLALAY
                     YNIENYSKAKELFEKCLSIKKDEEGVKALVLAYLSLGESENAVELAQKLSNEGFQGKN
                     LAYAIAKTFENKNSFSRETKNIDTAIDLYQSIILTDSENHNLYLSISICYAKKGDWEK
                     SVEYCKKALEKAPASYEVNNQMGLIYYCRNEVEEAVKFYEIALKAKPEGDYKIYSNLA
                     YAYEKLGEKDKAIKMFNSLLKKFPNYPAKDEIKNHLRILKNSD"
     gene            20939..21556
                     /gene="ruvA"
                     /locus_tag="PKI94_05105"
     CDS             20939..21556
                     /gene="ruvA"
                     /locus_tag="PKI94_05105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNW26153.1"
                     /translation="MYDYILGEITNSYQNNKGCFLVLESNKVGYLIETNSRILANLPD
                     ESKEYKIYTILIHKEDSMSLFGFLKREDRDIFKTLTSVSGVGPKMALMLLDEFEAADL
                     ISLVIRGEYKELTRAKGVGDKLAQKIILELKDKLINFDIKSDKIQKNTILTNNQHLQD
                     AQSVLISLGYTKEEINKAFLKAEPMIKNMDKAENILKETLKVLSL"
     gene            21650..22141
                     /locus_tag="PKI94_05110"
     CDS             21650..22141
                     /locus_tag="PKI94_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26154.1"
                     /translation="MNISCKPITNIVTARFNHTMTKPAVIPDISQKKDKLSFTGESDF
                     TVDSIGNKIPKSFENLMHHLVVVRKKDDIYVDNTNEGKIVTLYSNFKNPEVNYKSEYI
                     FSNDGTLKNVVKYIKTKNKYKLAEIRTYLQDGKTVLYTEITQCKKTDMCYFNPDGSII
                     TEK"
     gene            complement(22143..>22626)
                     /locus_tag="PKI94_05115"
     CDS             complement(22143..>22626)
                     /locus_tag="PKI94_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26155.1"
                     /translation="YPFYSLGHIIKNLKLVKRIKSKITGKDEVFVNTEKLVTDVLVTD
                     GKSNISCKLELISENGLAKVKFMFKKKRFVTSSHLRMYDALQELILKLADYGFVIKIC
                     QCCKNFTPNVDGSTNMVKGFCSNEFTTTTEGKIPTLLWNSCRGFSQSEIKSVIEEIAK
                     "
BASE COUNT         7634 a         3609 c         3772 g         7611 t
ORIGIN      
        1 acaatagcac ttataatcgg gcatttgact gtggcaaaac actgttttaa aaagcctgtt
       61 actcttgaat atcctgaaaa acaacctgtt ttaaacgaac atttcagggg cgaacatgct
      121 ttagtgagcg acgaaacagg aaaacttctt tgtaccgctt gtggtacttg tcaaagggtt
      181 tgtccttcct ttggaactat tgaaattgaa aaagaaaaaa atgccgaagg gaaattttat
      241 ccgaaaaatt actcgattga tttgaataaa tgcatttttt gcggaaattg tgttcaatac
      301 tgtcctttcg gggctattat aatgacaaaa aagtttgacc ttgcaaacga gaaaaagtca
      361 tctttaaaat tagacataaa tactttaaaa aagaactatt ctgataatat aataaagaaa
      421 gggttatgag taaaatcgtc atgctgaact tgtttcagca tctcaaaatc gtataaatga
      481 gacactgaaa cgagttcagg gtgacgtaga agaggaaagc agtggagata gatattttaa
      541 aaagcttaat attttacggg tttgcaggat taatcctctt gtttgctttt ttgagcattt
      601 tttcattcag gattttatat tctctgattt gtgcggtatg tgttttcttt gctaccgcag
      661 ggatgttttt cttacttgga gcggattata atgctgtcat tcaaatcgca atttacggga
      721 ttgcaattcc gattttgttc atttttgcaa taatgtttac ctccgataag cttgataaat
      781 ctgtttatat aacctttaaa cccagattct attttgcatt tatgtctgcg ggggttttgt
      841 ttttggcact gatttatctg ctcgcaacat ctttgggatt gagctcgcat tcaaattgga
      901 tattgcaaaa acaaacaatg tttataaaca agtttcaaat gtttaaagcc attgcagacg
      961 gatttttcac aaattacgtc gttgcgtttg aactgttttc aatattactt ttaatactgg
     1021 ttgtcggttt ttcgactttt aacattttaa aggagaaaaa cgatgactga tattttggaa
     1081 ttaatattta acataggttt atatcattat ttgcttttgg cgttaattct ttttattatg
     1141 ggctttttcg gcgcaatcgt gtctaaaaat gttttgaagg ttcttatttc cattgaattt
     1201 atgctgactg cggtaaatat taattttata gcttttgcaa gcttttttga caatataaaa
     1261 ctggaaggtt ttgtcttttc gctgttttat attgctctgg gagcggtcga agttgcaatc
     1321 gccttgtcga ttttttacct gatgttcaga caaaaacgaa gcgtcaatac tgaagattac
     1381 aaagaattaa aaggataagg ggtaaagtta agtacatgga ttttttcttt gaaaatattt
     1441 atacaattat gctttttcct atttggattt ttctgattat ttcaatcgga aaattcttcg
     1501 ggattttgga ttcaaaacct gcaatttcag gtttaactct tatttcaaca ctttgggggt
     1561 taatattttc aataggtatc cttcttggaa ttttgcataa tcccaatttt agttatgaat
     1621 catcaattac gtttttaaaa atcgaagatt atgcccttaa tttaggggta tatgtcgata
     1681 atatcgctgc tgttatgctt attttggtta cgggaataag ctttatcgtt caactgtatt
     1741 cttctgtgta tatgaagcac gatgcaagtt atccgagatt ttttgcgtat ttgaatttgt
     1801 ttaacttttc tatgctggga cttgttttaa gctcaaacct ttttcaaact tatgtttttt
     1861 gggaattggt gggagtttca agttaccttt tgattggttt ttggtacaaa aaaccatccg
     1921 cgtcaaaagc ggctaaaaaa gcttttataa tgaacagaat aggtgacttc ggacttttgg
     1981 caggcgttat ttcactttct tactttatgt atcaatactc agggttccct cagcttgcaa
     2041 caattccttt tgcttcaatt tctgatattg cagattacct tttctcttat acaaatgatt
     2101 ttgtttttat tacaatttcg atatttatgc ttatgggtgc gattgcaaaa tcggctcaat
     2161 ttcctcttca tacttggctt gcagatgcta tggaaggtcc tacacctgtg agtgccttaa
     2221 tccactctgc tacaatggtt gcagcaggag ttttcctggt ggcaaggctt tatccgattt
     2281 tcgtactaaa tcattatgtt atggacttta ttgccattgt ggggctcatt actgcattaa
     2341 tttgcgctta ctttgcaatt acgcagactg atttcaaacg tattttagca tattcaacaa
     2401 actcgcagct cggtttaatg tttttggctt taggtgcagg tgcttttacc ggcggacttt
     2461 tccaccttgt aactcatgca ttctttaagt caatgctttt cctttgctcc ggtatcgtaa
     2521 tccattctgt tcaaagccat caagatgtaa gatttatggg tgggatgaga aaagttcttc
     2581 cgattactgc actttgttat ttaataggct gtgtggcaat ttctggctta tgtttcagcg
     2641 gattttattc aaaagagctg attattaatg caatgttaag ttctaaaaac ttcatttacg
     2701 ttcaggcatt tttatttgtt tctttgatga ctgcatttta catgttcaga tcatatttcc
     2761 ttgtgtttga aggaaaatac agaggatctc acgagcctga aaaacagggc ggaattttga
     2821 atatctcaat tttaattctt gcaatattta ctgtattttt gggatatttt acaccgtttt
     2881 ttgaaaaaat tattccacaa atggctcaac ttccacaaat aaaatgggcg gttagtcccg
     2941 cttttgtttc aataagtata ggtattacag gatttttatt cgcttggatt ttttataatt
     3001 ccagagtaaa tgctttcaaa ttacccttgt tatataagct ttcttacaac aggttttata
     3061 ttgacgattt atacggtttt attgccgata agctatatta ttggacttca aggatttgtt
     3121 cgattatcga caaatatctg gtcgatggag ttgtaatttt atctgcgctt tgtacaagag
     3181 cgttttcgtg gacattttca aaaatgcaaa caggaaattt ccaaagttat ttggcatata
     3241 cttttgcatt tttggcgttt gcctttacgg ttcttatgtt tgcctatacg gcaatactgt
     3301 ctttcggatt attgagcggg tattaataaa ggagaataat gaattttcta tcagttttag
     3361 ttttaatatt ttcaccatta gtcgcatcag gactcatttt tacttcattt ttcaaaaaca
     3421 atgaagtgag aataagaaga ttttcaaaaa cttttgccgg cttgcatttt ttatatacat
     3481 taatgttttt ggtatttttt aaccctcaag ccgaaggaat ttcttactat aaggaaataa
     3541 ccttcttcgg ctcaggctgg attgagcctt tgggattgaa gcttgctttc ggacttgacg
     3601 gacttgcact tttaatggtc attttaactt ctttgattgt ctttttggct ttgattgcaa
     3661 gcaaaagcaa tattaaacaa aaacacaaat tttattattc tttgatattt ttgcttcaaa
     3721 ctgctgtttt aggggtattt tgtgcaaggg atatgtttgt attttttatg ttttgggaac
     3781 ttgaacttat tccgatgtac tttttaatcg gtcaatgggg cgatgctaaa gcaaaatctt
     3841 ctgcgatgaa atttgtttta tacactttct tcggaagctt attcatgctg cttggcattt
     3901 tattgttgca tttttactat tttgcgactt acggtgtgat gacaggcgat ttttcttcga
     3961 ttttggtaag cgcaccggaa tttccgctag cacttcaagt attgatttca atcctgtttt
     4021 taatcggttt tgcggtaaaa ctgcctttag ttcctttgca cacgtggctt cccgatgctc
     4081 acgttgatgc tccgacacct gtaagtatga ttttggctgc agttttactt aaaatgggtg
     4141 cgtttgggat aattcgattt aatatgcaga tgcttcctga tgcgtttaag gttttggtac
     4201 ctgccctttt gattatggca cttattaaca tcatttatac agcttttata gcgtattcac
     4261 aaacagattt gaagcgtgtt gttgcttatt cgagtatttc acacatggga attgtcttat
     4321 tcgggctttg cagtttgaat ttgatgtctt tttcgggtgc tgtgttccaa atgctttcgc
     4381 acggacttat ttcagcgggt ttattcatgg tagtaggtat aatttacatt cgtaccaaaa
     4441 caagacaaat atctgaattg ggcgggcttg ccgctgttat gccaaggctt gcaggattca
     4501 gcattattct aacacttgcg gctgtcggtt tgcctttatt aatgggtttt gtcggagagt
     4561 ttatggtctt tttaggctca tttaattcgg ctcttgatga tattttgcct ataaaattat
     4621 acactttgat tgccgtttta attcttgttt tgagtgtttg ctacatgtta aggcttttac
     4681 acgaaacttt ttactcgaat ttacttgaaa agtggcataa tttaaaagat attatgatgc
     4741 atgaattcac ggtactgtcg attattagct tttgcattgt cgtattcggg gtatttccga
     4801 tgtttataat aaatattgta cagccgcttt taaccggtat tttggaattt ttgagatgag
     4861 gagtgtctga tggaattttt taataacatt ttgaatacaa tgttaccgga aatagctatt
     4921 ttgttgttta tttttattct tataattttg tcgatgactt tggatttaaa gcattataaa
     4981 cttgcaaaat ggataacttt gcttggaatt tttactgcaa cggcactttg tacaaaaatc
     5041 caagtcgaac ccatagcata cggatttaag gatactttta tttcggatta ttatacgctt
     5101 ttttttaaat ttgttttatt aattgcatca tttataatag tttttcttac caaaaaggct
     5161 atcacgtctc gtcgtgacaa tgcttttcaa tttaatgcga ttttgcttac tgcgatttta
     5221 ggtgcgctta ttttagtgtc tgcaaacgat tttttaacaa tgtttatatc aatggaaacc
     5281 ctaggctttg caacattttt ccttcttgct tttgacaagg ggtataaatc caaagaagca
     5341 acttttaaat acattttaat aagcgcggtt gcaactgcgg tatttttatt cggcgtttct
     5401 tatatctacg ggcttaccgg cacagttaat ttctcaaaaa tttatgagta ttttttagat
     5461 gtcgatccta ctttgcttta ttcaatggca acaattttta ttgcatcggg actatgcttt
     5521 aaattaggcg taattccttt tgcaaactgg attttggatg tttatgaggg cgcagatact
     5581 tccgttgtta cttttgtgtc gattattcca aaagtagcaa tgttcagcat tttatcaaga
     5641 ctactggttt ttgcaatgag ttacagtttt gagttaacct ttacgctggt tgtaatctct
     5701 gttatgaccg ccctttgggc aaatactctg gctataaggc aaactaatat taaaagactt
     5761 cttgcatgca gttcgagtgc caatgctgct tacgttttgt ttgcggcaag tttggtttct
     5821 gtttttaact tatccaccgt acttttttat cttgtagcct atgtttttat gaatataggt
     5881 gtattttcgg cggtaattat tcttgaaaat tctcaatttt caaacagatt atatgaattt
     5941 aaaaactttg cttatactaa tcctattttc accctttgtt tttcaatttg tatattaggt
     6001 ttggcagggt ttccgataac ttcaggcttt gtttcaaaat tgtatttatt ttctgcgata
     6061 attcgctcag ggcttgtgtt tttaccattc ttaataatta tgatgctgac cattgtagcc
     6121 tcagtttttt actatttaac agttgtaagg cttatgtttg ataaatcgga taaggttgaa
     6181 agtgaagtta ttccgcacaa aacaagctca gcaatagtta tcctttattt ctgcgcttta
     6241 attactgtta ttatgggtat tttcccgtca aaagtaatag aactttgcca atttatagct
     6301 tataacgttt aaaattaaat atcgtcatac cgtgttgtat aatcaatgtt atgctgcata
     6361 aaaagaccgt catcaagtcc gcaatctaag tacataaata cttttctcaa agcacgtgaa
     6421 acaggtgtga gataaatttc gggttgggcg gatttatatt ctgtgggaac ttcattaatt
     6481 tggctttttt gaaggtctat taaccttgac atttcatcac tgtgcccatg attatctata
     6541 tatttttgtg atgaaatatc attgacactt aaatcagcaa atgccggtga tgataaaatc
     6601 agaacacaaa acaatgataa aaactttttc ataaatacct cttagagaat aatagactat
     6661 ttttcacaag attacaagtt tattatacaa tcttaacata ataatattat aggctatttg
     6721 agtgattttt ttataatttc gtcaagtttt tgtataattt ctgattccgg aactttacag
     6781 ataatctcgc cttttttgaa aatataacct tctccgattg caccggctac cccaaaatca
     6841 gcgtgcttgg cctcgccggg tccgttcaca ggacagccca taactgcaat agtgacaggc
     6901 tcttttaagc ttttgaattt ttcttcaacc tgttttgcaa gatttataag gtctatttga
     6961 caacgcccac aagtcgggca ggaaacaaaa ttaacccctt tttggcgcaa attaagcgat
     7021 tttaaaatct caaagcccgc ccagacttct tctacaggat tttcggtcag cgaaaccctt
     7081 attgtatcac cgattccttg agacaaaagg cttcctaaac ctacagaaga tttaactaac
     7141 cccgatttaa gcgttcctgc ttcagttact cccaaatgca aaggataatt gactttgtcg
     7201 gcaattagtt tatatgcttc gatagtgctt aaaacatcag acgatttgag tgaaattttt
     7261 attaaatcaa aatttaaatc ttcgaggatt ttgatgtgtt ttagtgcgct tttaaccatt
     7321 ttttgaggta aagatatatt ttctttggcg atttcttttt ccaaagagcc tgcgttaata
     7381 cctattctta tggggatttt ctgctgtttt gcaagtgtta caactttttt tatgttctct
     7441 aaattcccga tattaccggg atttaacctt agtgcatgaa ttcctttatt tatgcactca
     7501 acagccagtc ggtaatcaaa gtgaatgtct gcaataacag gaatttgaga ctttttaacg
     7561 atgtctttta tggcagaagc agctactttg tccaacaccg caaggcgaac aagttcacag
     7621 ccggcttcag tcagggattc aatttgtgcg agagtggctt tgatatctcg tgtatcggtg
     7681 ttgcacattg attgaacggt tattggggca ttaccgccga ttttcacccc gccaattaaa
     7741 atttgtttag attttcttct gtttatcata aaataatttt agcagaaata tacgtcatgc
     7801 tgaacttgtt tcagcatctc ttattaaaat taaggagttt aagaatgaaa gttgctataa
     7861 tatctgatat tcacggaaat atgcaggctc tggaatcagt tttggcagat attaaggcag
     7921 aaaaatgcga taaaatattt tgcttgggag atcttgcaat ggcaggtcct gagcctgtta
     7981 aggccgttaa attaattaaa aaaatggcag aagatgaaaa tttttattta attcaaggca
     8041 atacagatga atttatcgca aattattcac ctgaacttaa agaaaaaata gcctcaaact
     8101 tccctgtaat ggcagcagca cttgaaaatg acgtcaaaat tatgcccgaa aaagatataa
     8161 aatttttaaa aaatctgcct aaacagctgg aattgaaagt tgaagaaact aaaatccttt
     8221 tggttcatgg ttctccgaga aacaacggcg aaaacatttt cccggatttg cctatagaaa
     8281 aagtcgaaga aatgctcaaa ggcgttgatg cggatcttat tttttgcgga cacacacata
     8341 ttccttgcgg atatcaaaca aatacagagc aaacagttgt caacgacggc tctgtcggtc
     8401 gtccgttcac ttctgaacca aaagcctgtt acgttatagc cgaatttaaa gacaaaaaat
     8461 ttaatattat gcataaattt atcgattatg ataagcaaaa agcatccgaa atccttgcca
     8521 aaagaccctt caaagggagc gaaaaactgg ctcaaatgct tatcaatcct gaattaagac
     8581 atatttaacg aaatgtaata aatttgtgtt atttcgataa aattatataa tagtaatatg
     8641 tatgaattat acccatattt cactaatgac ggttctgtcg ggctatttag cagacaagac
     8701 gacgacatat accactctgc atacggtgct ctaagcgaaa gctggcagaa atttatcctg
     8761 cctgcgcacc tgcaagagta cctgcttaag catgacagcg ttaaaattct tgacatatgc
     8821 tacggaatcg gctataacac aaaaaccgcg ctgcaaactt ttttaaatgt aagcgaaaaa
     8881 aaatataaaa ataatttaaa aaataaaaaa gttttttcaa aaaatttttc ccatcctcac
     8941 actagtatcg cagcgataga tactgataat atttacagag aagatttcga gcaaatttat
     9001 actgacaccc ccttatataa cgcagagata tattccgata atatttcgag tgagaataaa
     9061 actgtaagtg aagaaaaaat tagtaaaatt tttatagatg ccgttgatac cgacaaaatt
     9121 ttaatgcaag tttctccatt tataacaaac gaaaataaaa attatttttt ctgtaacaaa
     9181 aacaattttt attttgaaaa aaattgcgaa aataaatttg aaaaaattaa aaaaataaaa
     9241 aaaaataaaa aattaaaatt agaaaaaaaa tataaaatca aaaatgaagt tttaattttg
     9301 atttttcaga aattaattca aaataacccc gaatttttcg acaaaatttt aacaagtatt
     9361 ttgtatcaaa aaaagatatc tccgtttttg tctaaattta tgctcaattt tgcccgtttt
     9421 tatcaaaatc aggggtataa taataacaaa aacttgaata attcgacctt tttacataat
     9481 atatattata ggtatatatc taaaagttat aaaaatgcaa aaaaatacga taaaaacaac
     9541 aaaatcacgt taaacttcca ccattttgat gcaagaaagt acgtaaaaac aaccaaaaac
     9601 acctatgact ttatatttct ggatgcgttt acaccttcta aatgtccttg cctatgggct
     9661 ttagagttct ttgaacagct ttatagcaaa ttagaagatg acggcatgat tttgacttac
     9721 tcaaattctg cggcggttag aaatgctttt cttaaaagcg gattttttgt cggtaaaact
     9781 tttgacagta aattaaataa attcatcggt acggttgctt cgaaaaatcc tgatcttata
     9841 gaacataaac ttaatgaaca ggatttggag cttatgaagt ctaaagcagg tatatgctac
     9901 agggatgaaa acctcgcttt tgataatcat gttattcttg caaacagaga tatggaaatt
     9961 gaaaggagcg aacttaaatc aagctcgcag gttttgaaag ggactaacca atgacctcag
    10021 ataaatatga tgttattata gcaggcggcg gcacagcagg ctgtgccgcc gcctatacct
    10081 gtgccaatct cggtttaaaa acccttttaa tcgaaaagaa tattcatctt ggcggcacaa
    10141 tgacctcagg gcttgtaatt cctgcgatga attcatcgaa aaatcaaatc aatactgatt
    10201 tttttaatgc tttgatttcg gaattagctg atttaaaagg tcaaattact tacgaaaata
    10261 attcgggttg gttcaatcct gaactttgta aaatcgctct tgacagatta atgtcaaaaa
    10321 gcggagtaga agtgcttttc gacactagaa taataggcgt taatgctata aatagaaaaa
    10381 ttaacggtgt tacaatttct tcaaatatgt tatcggtata tatcgcgacg agtaatgtta
    10441 tagacgcaac gggaaattgc gaaattggaa aaattttggg ctgtaatttt ttagaaaata
    10501 aaaatgaatt tcaacctgtt tcgcttcgtt ttcaaatgag cggaattgat ttagatattt
    10561 ttggtaaatg gctattagac tttgataaag acaaaacagt tacatcaatg cacgttatag
    10621 acgggcaggt gcatttgtca acagcttaca catgggataa ggataaaaac tgggctctaa
    10681 agcctatttt tgaagatgct gtgacaaaaa atgtgttaaa agacactgat agaagctatt
    10741 ttcaagtgtt ttcaatccct aatatgccaa attcattggc ttttaactgc ccaagagttt
    10801 attttgacaa agaaattaac cctctagaca atgttaatat atcaaaagcc ttaatagttg
    10861 ggcgtgagag cataataagg atagccgatt tttgcaagat ttacttcccc ggatttgaaa
    10921 aaagttatat ttcaaacatt gcagatgcac tgggagtacg cgtttccaga cgtattaaag
    10981 gcaaatatgt ttatacggtt gatgatttaa aatcgggtaa gaaatttaaa aatcctgtct
    11041 taatatcgaa ttatccgatt gatgttcact cgaacaaaga cggcaaatca attttagaaa
    11101 agacagaaaa agaatatcaa ctgcctgttg aagcccttat atcagctgat tatgacaatt
    11161 ttttcgttgc aggaaggtgt ttaagtgcag atttcttcgc tcaagctgct ttgagaattc
    11221 agccaagctg tttttctatg ggagaaggct tagccaaaca tattgcctct gtggttaatt
    11281 aattttttta tcctttatta tacactaacc tctgtagaag gagggtgtta tgggtgataa
    11341 aaaacttgac ggtacaagga ctcaacaatg ccttataaac gcctttgcgg gagagtcaca
    11401 ggcaagaaac agatatactt actttgcttc tgttgccaaa aaagcaggtt atgaacaaat
    11461 ttgcgcactt tttttagaaa ctgccgataa cgaaaaagaa cacgccaaac ttttttacaa
    11521 atatttggga agcggcgttt ttaatgttca tgccgattat cctgttcatt tgggaacctc
    11581 tttggaaaat cttaagcacg cggcagcagg tgaacatgat gaatggtcaa atctttactt
    11641 aaatgcttca aaaattgccc aagaagaagg attttatgat gtggcggaaa catttaaaca
    11701 tgtttgtata gttgaaaaac atcatgaagc aagatttttg gagcttgcta aaaacattga
    11761 agaaaatacg gtctttaaaa agttgtccga agttaactgg aaatgcagaa attgcggatt
    11821 tattataaaa ggagcagatg caccgctaat ttgtccaact tgttttcacc ctcaagcata
    11881 ctttgaatta ctctgtgata acttttaatg cttaacatat tgataaaaac cgactgtttt
    11941 tatttattaa ttttaccttt gataatataa ttatatgaat gaatgcaaga cttaaaacat
    12001 tattatattt attttctttt gtaattattt atgcctttta ttattgggga cttccagccc
    12061 ttataaatat taaacataat atatttgtgc tggaaaatat tgtaaataaa gaattgggca
    12121 tacaaattaa atttaaaaat ccgcagttaa aaatggggct tgtgccttca gtgtggatca
    12181 atgcggacga atttaacgtt ttagatgcaa atacttcgcc gctcacagtt aaaaatccga
    12241 aattaaaaat acgacttttc ccgttagtaa taggtaaaat tcaaccgtca tacttttcat
    12301 gcgacaagct tgatttgaat attaaagtcg ataaacatct tcggctttat ttgggaaata
    12361 ttctttttat tgaaaaggca aatcctaaac tttcgtttga ggacatgcaa acttatattc
    12421 aggaatataa tatattctta aatgacgaat tccaaggcaa aaacattctt ttcagtggcg
    12481 attattttaa tttatataat tttaattcaa aaaagaacat taaattatcc acaaattcaa
    12541 agataaaagt aaataataaa acctctgtaa ttgacattga cctggatttt aaacttcctt
    12601 tcaaaaaaag ttttgaaaca aaagatgttg tttttgacgg tactgtaacc aaccttgatt
    12661 taggcatttt tgcaccatac attaaaaaat tcacaaaaga aagaatttta aatactcaag
    12721 gattaataaa catagaaact aaaacaaaaa ttttagactc ctctaaaaaa agaataaact
    12781 ccttaattag tattcaaggc ttaaaaataa tagaaaagga taaaccttca tcaattattt
    12841 ttgacaaaaa acttgatttg acagctattt tggatgtttc aagagggcta ttaaacataa
    12901 acaaattcaa aattaaatcc gataaaatag ataccgaaat ttttggtgaa attcaaagaa
    12961 ttaatgctca aaaccccacc ctaaatctta atataattgt tgaaaaatca agaattgaag
    13021 atttaatatc acttatacca acagatttta acatcaaaga agtaaacctt tatgcattga
    13081 aaaaatacgg gtattattct gatattcaag gcaatttatc aataagaggg cgggcggaca
    13141 gaccaaattt aaaaggaaaa atcatctcca caaatgctta tgtaataaag ccattaccgc
    13201 ttcatatccc taaagcaata gtcagccttg actttttagg tgaaaaattc tttttagatg
    13261 tttttgtacc atgtgaaaaa gcagaacatg ttagcgttaa gggcgatatt tatttatatg
    13321 atgataaaaa aacttatttg gacattacaa gtacaaaaaa tgttaatttg aaaacagcgc
    13381 aaattgtcct aaacccaata cacgaaattt ttcaatttga ccttggaccg cttcctgtta
    13441 tggatttaca cggatttggc aacataaatt taaaaacagc cggaacaaaa gcaaatccga
    13501 gcttgtttgg cgtttttaac tttagagacg cgagcgttaa aattaacgga cttgaaatgc
    13561 ttttgacaaa tgcaaacggc aaacttgaat tcaaaaatgc caatacttat tttgaaacaa
    13621 gtcaagcata tttaaacaat aaacctgcta aagtcaaagg tacatgtaca ttatctggcg
    13681 agcttgatta cagcgcagtt gccaacggtc aagatttggg cgatttaatc tatattctaa
    13741 aaaattctcc cattcttggc gatatgcaaa aacaggtttc tcagcttcaa aatggaaaag
    13801 gcaaaattga ttttacctta aaattatccg gtaaagttaa gtcgattaat gatttcaaat
    13861 taggaaaaaa cgtttttgta caaggtacca tcaagttatt tggcaataac gccagcatcc
    13921 agggactttc tcttcaggtt aaaagtattt taggaactat taaatataaa aacacagatt
    13981 tagaagttga tttgttatca aatttaaata aatcgaaggt taaaatcgaa ggtaaaattg
    14041 atgataacaa cgcaaaactg aattttaagt cggataagct ttacatctca gatattattg
    14101 atgttttgcc ctctttaagt tttgataatt ttaaatatat aagtacaaat tcaaacaatt
    14161 acattaacct gaaagcgtct tatttcgggc ctgtaaataa aattgatacc gacaaaattt
    14221 ctatttacgg taaaactaaa ataaataatg ttaagtttac atacctaccg caaaatttgc
    14281 ctgtaaatct ttatagcgga gtttttgaaa ttaaaggaaa cactataaat ttatataaaa
    14341 ttaacaccac ccttgattct gttcctcttt tggttgacgg aacaatatat aatattttca
    14401 aaaatccgaa ttttaacatt tatgttaact ccagaccaaa tcaaaccttt attgacaaat
    14461 acataaatac aaaagcaatt tatccggtaa aaattaaagg cgacattaca ttttcctcaa
    14521 aaattcaagg caccaaaaag cttttttcaa caaaatcaga aataaatctc caagacgggg
    14581 caaatattta ttacatgggc tctgcattgg gtgattcaca aacgccgatt aaaatcttct
    14641 taaatgcgga tattgcaccg acttatatta atataaataa ttttcaatac gataaactta
    14701 tttcgtcaca aaatggtaaa gaattcgttc aaaatcagct ttcggcaaaa ggtttagttg
    14761 atttcaacag aagtaaaaaa atccttcttg aaaattttta tgttaaaacc tctaatccga
    14821 ccgatgcaaa aatattcaac atgattttta gaaaaccctt aataaaacaa ggtcaattta
    14881 catccgacat tgctattaac ggtgacctat caaggccaag aatgattgga gaccttgatt
    14941 ttacaggcat agacattcct cttttggata caaccgtcaa ggacatttca cttgattttt
    15001 tcaatgaata catcagtata aaagcaatcg gtgaaatttt ctcaaataaa attctgcttg
    15061 aatcaaccat gaaaaataaa cttgaagtac cttatacttt caatgaaatt aacgtaacta
    15121 ccgataaact tgatataaac gctattgcaa aaagtatttc taatcttcaa atagaaagtg
    15181 ataagcataa aataacaaat gagaaccaaa gcatagattt atcaaatctg ataataaata
    15241 atgcgcagct taaagcaaca aacgtgcttg taaaaaatgt tcaggcaagc aacttaattg
    15301 ctaaaatgtc tttagatgaa aaaatgcttt tcagccttga tgagtttagt tttgatttag
    15361 gcgaaggtaa agttaaaggt aattttaact ataatctgct taactcaaaa tcaaagttga
    15421 acttaaattt tttaaacgtt aatgcaaacc aagtggcaga atcacttttt gatttatcta
    15481 atcaaattta cggaaaactt aaaggcgaag ctgaaataat gtgcaatgca aagtcagaaa
    15541 aactttgtat ggaaactata tcaggtcagg gctggtttga agtcgcaaac ggcaaaatgc
    15601 cgaaattagg ctctctcgaa tatcttttaa gggcttcaaa tgttgttaaa agcggtatta
    15661 caggactttc tatcaataat ataattgatt tgctcactcc tcttaaaacc ggacatttcg
    15721 ataacatcaa cggcaatttt gatattgatt cgggcttcgc aaaaaccatt caaatttatt
    15781 cgaaaggaaa agatttaagt ttgtatttaa caggcactta taacttttcg actttaattg
    15841 ccgatatgaa tattttcgga aaactttcta aaaaaatatc aactgtattg gggcctgtag
    15901 gtaatgcgtc tctaaactct gtgtttaatg ctattcccgg aataagtatt gacgaagcaa
    15961 aacaatccaa actatatagt gaaatgataa aaatacctga ttttgaactt aatgatgaac
    16021 tatacagaat attcacggtt aaaatttacg gtgatataaa tggcgaaaac tatgttcaaa
    16081 gcttcaaatg ggttgaataa aatggaaaat tttattattg acacacactc tcacatagac
    16141 atgcaggatt ttgaaaatat caatgaaata attcaaaacg ccaaaaactc aggagttaat
    16201 aaaataatag ttccctctgt tgacaggagt tcttttaaaa atgttctcga actggcgcaa
    16261 agttatgaaa atgtttactg tgctttggga atccacccga gtgaagctca aaatgctgcg
    16321 gataaagatt ttgaagaaat tttaaaactc ggaaaagaaa aaaaaattgt cgcaataggt
    16381 gaatgcgggc ttgattatta ctggagtaaa gattttattg accaacaaaa aatcattttt
    16441 ataaaacaaa ttgatattgc taatcaatta aaaaagactc ttatcgttca cgacagggac
    16501 gcccacaaag atactttcga tattttaacg gctaatatca aagatataaa tgtagttatg
    16561 cactgttttt ccggaagtta tgaatttgca aaagagtgtg ttaaaaaaga tttttatatc
    16621 ggaatcggcg gtgttgtaac ttttaaaaat gcaaaaaaac ttcatgaaat tgcaaaaaat
    16681 attcctttgg attatatttt acttgaaacc gatgctcctt atttaacacc ggtgccattt
    16741 agaggaaaac gaaatgaacc tgcttataca aaatttgttg cagaaaaaat cgcagaaata
    16801 cgaggaatat caacagacga tgtgataaaa ataacgacct caaatgcaag aaaggttttt
    16861 ggaatttgaa aaaagtaaga ctggacaatt ttttagttaa tttgggatac tttgataata
    16921 aaagcaaagc atctgctaat attatggcgg gcaatgttca agttgatgag gttattatta
    16981 caaaagcagg acatcacctt gacactgcta aaaattatga aattaaagtc aaaacaacac
    17041 cttttgtttc aagaggcggg ctaaagcttc aaaaagcagt agaaactttt aatgtagata
    17101 taaaaaacag aatttgcctt gatgcagggg catcaacagg aggctttacg gattgtcttt
    17161 tgcaaaacgg agcgcaaaaa atttatgcag tagatgtagg atacggacaa atcgactgga
    17221 agctcagaag caatgataaa gtgaaagtta ttgaaagaac aaacataaaa aactgcgatt
    17281 tttctcaaat atatttacct aaagaaccta tcgcagacct tgcggttatg gatttgtctt
    17341 ttatatcctt aaaaaaagtt ttacccaata ttaaaaccct tttaaaagaa aatcacgaaa
    17401 taattgcgct cataaagccg caatttgaag tcggcaaaga acacattgca aaaggcgggg
    17461 ttgtcaaaga aaaaaatgct caggcaagag cgatatacga tgtgatagat tttgcaaaaa
    17521 ctttgggcta caatgcaatt gatttgactt attcaccact aaaaggccct gctggaaatt
    17581 gcgaatactt aatccacctt tcaacggaaa ctgcaaaaca acaagacttt ttaatcaaaa
    17641 atattgtaaa cgaggcaaat acatgaacac aaaattgatt gaaaaagcag tatatgaact
    17701 ttgcatacaa gcaaacactc agctttgtga tgatgttttt gctaaaatat gcaacgcctg
    17761 cgccaacgaa acaaacaaaa attccaaaca agcactagag ctaatcttac aaaatgcaaa
    17821 aatgtctttt gaaaccaaaa aaccactctg tcaggacaca gggcaagttt tggtattttt
    17881 aaaaataggc gaagatgtta agattaaaaa tcttacaaaa tcaataaaca acgcggtttt
    17941 taaagcttat aaacaaaatt tttacagaaa atccatagtt aaaaatgcac tttttgaaag
    18001 gataaataca aacacaaaca ttccgtgcat tatatacaca cagattgtaa aaggcaaagg
    18061 tcttgaaatt gaattattaa ttaaaggagc aggtgcagaa aatgcatctg cagcaaaaat
    18121 gctttctccg actgccaatg aaaaagaaat tatcgatttt gttattgata ctgtaaaatc
    18181 agcaggtgca aaagcttgcc cgccgttttt tataggaata ggaatcggcg gaacaattga
    18241 atattcaggt cttttatcta aaaaagccct tcttttagat aaaaatattg acgaaaatca
    18301 cgaaaaactt gcgcaaaaaa ttcaaaataa aataaataat ctaaacatcg ggacaatggg
    18361 cttagggggc aattttacgg ctttggatgt aaaaatactt acaagttcga ctcacatcgc
    18421 gtctatgccg attacagtga ccgtaaattg ccacagttcg agacatgcta agtgcaaaat
    18481 tgaaaataat aaaattaaat tttacaattc aaaaaaaata agaaactata aaccatttga
    18541 aaataaattc aatgactata taaagctaaa taccaatgaa ataaataaaa tccgaaagct
    18601 taaaacaggt caaaaagttc ttcttacggg tgaattatac acagcgcgcg acatggcaca
    18661 caaaatgctt gttgaactga tacaaaaagg caaaaaattg cctgttgagt taaaagataa
    18721 aataattttt tacgcaggcc cctgcccaaa tccgaaatct gaaataatag gctcggtcgg
    18781 gcctacaact tctgcaagaa tggataaatt tatgcctgag atttcaaaaa cagaaatttt
    18841 agccacaata ggaaaaggcg aacgttctgc tgaagcaata aaattaatgc aaaaaaataa
    18901 aattataaat tttggagcaa tcggcgggat tgcctgcctg ctttctaaaa aaatcacaaa
    18961 aaaagaaatt attgcttttg aagatttagg cactgaggcg atttacaggc tctgtgtcca
    19021 tgattttccc gtttatgttt cttgattcat ttgcataatc gactagtgcc ttaaacagat
    19081 aggggtgaac ctctcctttt ttcatttttt tgtgaataat ttcaagtgcc ttatttattc
    19141 ttaatgcctt tttataggga cgcttttcta ttaacgcgga aaaagaatcc gcaacagata
    19201 aaagttgtaa atttaaatcc acaacttcac tattagtcct tttgggataa cctgtattga
    19261 aaggattttg gtgatgatat cttactaaat ctaatacttt tggagataaa tcagtcgttt
    19321 ttaacatttg gtatccaagt tctgaatgaa gttttataat attaaactcg tttaaagaaa
    19381 gggcttgggg cttgtttaac aattttgcgg gaattaaaac ttttccgata tcatgtaaca
    19441 ttgatgcttt ttgaacagat tcaaaatcaa tcgcatgacg atatttgtca tctaaatgct
    19501 gataaatacc taaaagtgtt tctctggttt ttgaaagatg agttttaaca agattattta
    19561 attcatctgt attaatcttt accggaagat tgttactttt taaaacctct gatattttag
    19621 gatttaaact aagcatcctg tttacagcca tcggtgttaa atattgttga atttcattat
    19681 taaaactgaa tgaatcatca aaattggacg caaatgaaat tttactgctt ttatatttgg
    19741 gcacagtaat aaaagcagta ttatgtattg tattcaagct taattttgag tttacatcca
    19801 accctgtcat taaaaatctc acaccacatt cacactatat atttatataa aaaaaaatta
    19861 tgcggtgatt ttgccaaatt aacaatattt ttaacaaaag gtaacaatta atcggaattt
    19921 tttaaaattc ttaaatggtt ttttatttca tcttttgccg gataattggg gaattttttc
    19981 aataatgaat taaacatttt tattgctttg tctttttcgc caagtttttc ataagcataa
    20041 gctaaattag agtatatttt gtaatcccct tcgggtttag cttttaaagc aatttcgtaa
    20101 aacttgactg cctcttcaac ttcatttcta caataataaa ttaagcccat ttgattgtta
    20161 acttcataag atgcaggggc tttttcaagt gcttttttgc aatactctac ggacttttcc
    20221 caatctccct tttttgcata acagatagaa attgacagat acaaattatg attttcagaa
    20281 tcggtcaaaa ttatactctg atacaagtcg attgcagtat caatgttttt tgtttcacga
    20341 gaaaatgagt ttttattctc aaaagtcttt gctatagcat aagcaagatt tttaccttga
    20401 aacccttcgt tagaaagttt ttgcgccagt tcaacagcat tttccgactc tcccaatgac
    20461 aaataagcta aaacaagagc ttttacgcct tcttcatctt tttttatgct taaacacttt
    20521 tcaaacagtt ctttagcttt agagtaattt tcaatattat aataagcaag tgctaaatta
    20581 taaagcccgt cataatcttc gggtttagat tctaaaaact tattataaga tgtaactgct
    20641 ttttcataat tccccaaata atgatgagtc aagcccatat tataataaac ctgaggctga
    20701 ggatgttggc actcaagagc cgttttaaac tccaaaaggg ctttttcata tttttttttg
    20761 ttgaaataat caatgccgcg attataataa aactcaaacc ctttattctt ataaaggatt
    20821 ttttctgcaa tatcggtgat tttttcaatt aagcttattt tactcataat tttattgtat
    20881 tataataaaa cagtaaagaa aagtatttta taaatcttaa aagaatttgg agaaagcaat
    20941 gtacgattat attttaggag aaataacaaa cagttatcaa aataacaaag gctgctttct
    21001 tgttttagaa tcaaataaag taggctattt gattgaaaca aattcaagaa ttttagcaaa
    21061 tttgcctgac gaaagtaaag aatacaaaat ttatacgatt ttaatacaca aagaagactc
    21121 aatgtcttta ttcgggtttt taaagcgcga agacagagat atttttaaaa ctttgacttc
    21181 tgtatcaggc gtaggcccta aaatggctct gatgcttctt gatgaatttg aagccgccga
    21241 ccttatttcg cttgtaataa gaggggaata caaagaatta acaagagcca aaggagtcgg
    21301 ggacaagctt gcacaaaaaa ttatacttga acttaaagat aaattaatca attttgatat
    21361 aaaatctgac aaaattcaaa aaaatacaat cttgacaaac aatcaacact tacaagatgc
    21421 tcaatcggta cttatttcac taggctatac caaagaagaa ataaacaaag ccttcttaaa
    21481 ggcagaaccc atgattaaaa atatggacaa agccgaaaac atactgaaag aaacgctcaa
    21541 agttctttca ttgtaaattt taattaactt tttatatttt tcgcaaaatc aatggcaatt
    21601 ttatttcttt acaacctttt aattgttata tttaaaggag aaataaacaa tgaacatcag
    21661 ttgcaaaccc attacaaaca tcgttactgc gcgttttaat catacaatga caaaacctgc
    21721 ggttatacct gatatttctc agaaaaaaga taaattaagt tttacaggag aatctgattt
    21781 tacagttgat tcaatcggaa ataaaatacc caaatctttt gaaaatttaa tgcatcacct
    21841 tgtagtggta agaaaaaaag atgatattta tgttgacaat acaaatgaag gtaaaattgt
    21901 taccttatat tctaatttta aaaaccctga agtaaattat aaatcagaat atatttttag
    21961 caatgacgga acacttaaaa atgttgttaa atatataaaa acaaaaaaca aatacaaact
    22021 agccgaaata agaacatatt tgcaagacgg caaaacagtc ttgtacacgg aaattacgca
    22081 atgcaaaaaa accgatatgt gttactttaa tcctgatggc agcataataa ctgaaaaata
    22141 aatcatttag ctatttcttc gattacgctt ttaatttcgc tttggctaaa gcccctgcac
    22201 gaattccaaa gcaaagtagg gattttacct tctgttgtag ttgtaaattc attgctgcaa
    22261 aagcctttta ccatatttgt cgaaccatcg acgtttggcg taaaattctt acaacattga
    22321 cagattttta ttacaaaacc gtaatcggca agtttcaata taagctcttg aagcgcatca
    22381 tacatcctta aatgagaaga tgtaacaaat cttttcttct taaacataaa tttaactttt
    22441 gcaagtccgt tttcggaaat aagttcaagc ttacaagaaa tattgctttt tccgtcggtc
    22501 acaagaacat ctgtgacaag cttttcggtg tttacaaaaa cttcgtcttt gcctgttatt
    22561 ttactcttaa ttcgctttac aagttttaaa ttctttataa tgtgtcccag agagtaaaac
    22621 ggataa
//
LOCUS       DAOQAU010000026        22041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2403_length_22041_cov_7.309515, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000026 DAOQAU010000000
VERSION     DAOQAU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 22041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22041
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2403_length_22041_cov_7.309515"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..567)
                     /locus_tag="PKI94_05120"
     CDS             complement(67..567)
                     /locus_tag="PKI94_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26156.1"
                     /translation="MFKRLTALIIVLTAFSVQMVFAEQTYTLKTGISVIDRVPNMLYG
                     TWRVSSTRTSTNSQATFKPSTVDLWNLSREGNVLTLENPFSGAKASVEINEVSDRLIK
                     FKKIGDFDGKKLTDIVELNLGKDSFEGVNTLLLETVSDVDKTVIKSERATYKLVGEKI
                     AGSSIK"
     gene            complement(540..1427)
                     /locus_tag="PKI94_05125"
     CDS             complement(540..1427)
                     /locus_tag="PKI94_05125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW26157.1"
                     /translation="MEGFKIKVSVTIPTYNNLESFKRTLNSVLCQTFSDFEIIITDDS
                     ENDDIKIYTALLNDERIKYFKNPQKMGAPKNWNEGIKKAQGEYVKILHHDDWFADENS
                     LKKFVELMDKNPQCDFGYSKSVDFNVETKKIKTRKAEKYVSKIEKNPFELYLCNRIGS
                     PSVVIFRNKNDLLFDENLKWLVDLDLYIEALIRNNKIAFINKVLVNIGISQAQITNSC
                     QDNQKVEIYELFYLYNKYKKNLDNNKYYPKLTEALKRFKICTLEKLREIIPQEFEIPE
                     RIINKLSGKKKCLKDLLHL"
     gene            1492..2316
                     /locus_tag="PKI94_05130"
     CDS             1492..2316
                     /locus_tag="PKI94_05130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26158.1"
                     /translation="MNKKVSVITASYNYAEFLKEAVESVIAQTYTNWELIVVDDGSTD
                     NSVEVIKEFCARDERIKFFRHLEGENRGLKETLILGIEKATGDYIAFLESDDIWEQNC
                     LEEKIKVAAQYPEAKIIFSDVDLFGNEEIIEDYARYFELNDRILSSKKYPANLFSAFS
                     FQNVIPTFSCAVVEAETLKKADFNPTVEAFLDHWVWAHLAYDNKFYYINQRLTRWRMH
                     TTSLVGRRQNKNEKQFLSDILKSFQKKGFNKNMFFWNRHRHPKIEKIFRGLMRRFV"
     gene            2399..4984
                     /locus_tag="PKI94_05135"
     CDS             2399..4984
                     /locus_tag="PKI94_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNW26159.1"
                     /translation="MKKFLVLLFVFCLGVSAFAQDYQIYKMESGQTVIIKQVKNNPIV
                     TIDTWIKTGSINENDKNNGVSHFLEHLFFKGTKNHPTGEMDRLLESKGAVQNAATSKD
                     FTHYYITIPSKYFDLALDLHSDMLLNPLIPRKELEKERKVVMEEIAKDENDPSNKVYE
                     NLIQMLYTTHPYKRKVLGTNDVIGKITREEILNYYKQYYNPQNMVTMVIGDVEPEDVL
                     KKISQEFKQKEVKKSSKISNKKEKQLCQKIVKVDYQPVQSGYMLIGYRAADAMNLDSY
                     ALDLLGIILGDGRSSILYQSIKEQKQLAYSIAAENSTFKEDGIFMISANFTADKCEKL
                     QRAVFDEILKIQKNGVTPEELSKAKSALEIETYYARESISNIASSIGYTTVLTDNPKY
                     YEEYLENIKKVSLADIKRVANIYLGENQCAVSIVLPEQEEQAKAQAAKTEHNIKMINQ
                     TSGVQKYEIDTGAILLITPNDLNDIVAISIYCKGGGFVEKIKGTAFLTSVVMLKGTKN
                     YSSLELAKIMEENGIKIQPNPTTDSFTINVLTTKPKLQKTLEILDEVVNRASFDDYEI
                     EKTKTAKLNSIKQSRDVPLNVALEEYKTLIYENSPYSTSPKILEKNLPKIQREDILQY
                     YSQVFNPKNIVISVNGKAELSDVANQLGDIFGTKAGEKFSYESYTSKIPPISGTKTST
                     KNIKDLQTAWLVIGWQTSGLQNQKDYVTLQVIDAILGSGMSSRLFKNLREQEGLAYQL
                     GSSYSPNVLRGTFTMFIGTNPKTLALSQKMLLAEINKLKTQFVGDKELSEAKEKIIGN
                     YLLSLETNSEKASNIGWFEASGRGFEFKDKYESLINSVSASDIVEVANKYFNQDYVVS
                     VVNSN"
     gene            complement(4981..5760)
                     /locus_tag="PKI94_05140"
     CDS             complement(4981..5760)
                     /locus_tag="PKI94_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNW26160.1"
                     /translation="MELILSQFAKLFPAFNSAIAAGIIVFARMMGFIRFCPVFNRKEI
                     PTLVKIALALLLTIMMTIVLKPSAPPASNSLFLSIVLNIAVGAMIGYMAQLILIAIDA
                     GADMINMQMGLSSAMVLDPTTQSQVSILEKAFSLLGVLIFIQLGGIYWLFQAFMRSFE
                     LFPIYATQIPLTKIVNMEYLSQMTSNVLYMGLQVASPVLLATLGQDIILGIISKTAPQ
                     VNVFQLSFLFKPVLGAAILVWILPMLVTIISDYFLSFSHII"
     gene            complement(5760..6173)
                     /locus_tag="PKI94_05145"
     CDS             complement(5760..6173)
                     /locus_tag="PKI94_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HNW26161.1"
                     /translation="MVKKIILLTIFLLFGTNCFALESFEIHLGKSYILSTQSIVETIA
                     VSDSEIVNVSPFFTIFNEKNVILVQPLKMGKSNLTIFTGKGDVKFEITVKPSVKYEEL
                     PRLQNEIFELLLLDTPPVIDEIKLDEHPIKFERSE"
     gene            complement(6210..6698)
                     /locus_tag="PKI94_05150"
     CDS             complement(6210..6698)
                     /locus_tag="PKI94_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HNW26162.1"
                     /translation="MELLIEYMARGFVIMLLISMPCVLVAAGIGLVVGILQAVTQVQE
                     QTIAAAPKILGVFLVIIIGGIGFVRLLTNFFTEGVAVAFNVVPKSDSYVLPSDYYKYT
                     RPFAGEMNDTIKDNPDIQDLINKSGKSQFENRQEKFKYSPAPKSTQPRPNFVETKKIL
                     GR"
     gene            complement(6876..7517)
                     /gene="fliP"
                     /locus_tag="PKI94_05155"
     CDS             complement(6876..7517)
                     /gene="fliP"
                     /locus_tag="PKI94_05155"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNW26163.1"
                     /translation="MDTVLKDMNPVIQMLIVMTVFSLLPFVFSCMTSFLRFTIVFSML
                     KTALGTQQVPPGIVIIGLSMILTFYTMGPVFQKMYELGSVPYNKNGNIVLAIEQGSKP
                     LKEFMMKQTRESDLEFFVSLSQKNPPKSPEELTIWQVAPAFIISELKTAFEIGFIIFV
                     PFIVLDLVVANILLALGMFMLSPTIISLPFKLLIFIAVDGWSLIVQGLVNSYN"
     gene            complement(7517..7948)
                     /locus_tag="PKI94_05160"
     CDS             complement(7517..7948)
                     /locus_tag="PKI94_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNW26164.1"
                     /translation="MTGYLVNFSIYTMAMIGVIFIALFVFKFVSGTGSFSKKSGFLNI
                     EDSLNLSARKTLFVIRAGEQRFLVASDIDRTALISPLEEKSLTTEPNIRQDRSSELPT
                     FDGVESIRDMASIIDFKKARKSKGPVMKELAEKLKAMQGSN"
     gene            complement(7999..9156)
                     /locus_tag="PKI94_05165"
     CDS             complement(7999..9156)
                     /locus_tag="PKI94_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNW26165.1"
                     /translation="MNNTYTDTKLTDKNELYAQYNWLSSTFSPKIQESSDNFFSELFD
                     YKLVSVSKNQNILFKGEDYFVTKVRFNKQNEVFLRISETGVKSILNKVLGPSEKAFSM
                     TALTELEAKIITAFNEHAYKNISQFFSPQNSNQSHDITHLTFFFKSKDGSDFGKYIIS
                     IPQTLLTPQEAQNLQENFNVSDFSKSLVKVKIKVGTTKFYVKELKNLEKEDLVIFEDS
                     NIQQMELIYKDYKQNFRVTPNPGLITSLENINGGHNMNENALSQELWDNIQVEMDAIF
                     DSVKITLGELKSIEQGLVVDISSVYDNKILLKVEDKTIAKGELVIINDRYGVKIDEVF
                     ATQKAQAQQEISQTNAEVITEQGATEAQVHEQEGAEEEFDYSDFELDEQDV"
     gene            complement(9261..10094)
                     /locus_tag="PKI94_05170"
     CDS             complement(9261..10094)
                     /locus_tag="PKI94_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNW26166.1"
                     /translation="MKKLTYLFLTVFIIMTAFGNYSYAQNIKFIQITDSHFSKNSTEY
                     TQREVENSEKFLDNTLQDINSIKNVDFVVFTGDNIDTANEIDLKAFLQKANKLKVPYY
                     IVIGNHEVFKSQNFTKKDYMKIVRRHSRCCKPWGANYVFKKNGFTFIVVDGAKEVIPG
                     PSGYYKKATLSWLDKKLTQYENENVIILQHFPVEAPYYNRTHSTYKVQDYQAVLKKHH
                     NVIAIVSGHYHANGEIKKDGIYHISTPALVEYPHNFKIIEIVQKNKKDVQIYTQLRHA
                     Q"
     gene            10183..12012
                     /locus_tag="PKI94_05175"
     CDS             10183..12012
                     /locus_tag="PKI94_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNW26167.1"
                     /translation="MKDFLHNASGGLEKNVFAKVVNFFKGMINNHPEMFAVLFLFFMC
                     LLFLFFGLNFYPLIDVDETRYAVIAKNLIGSRDWNSLILNYEPFLEKPPLYFWLVAAS
                     VKFFGGLSAFAIRFPMAILASFLTFFTYFFGKKVISRKFGIVSALVLLTSIFFLILSH
                     VAILDIVVTVFVASALYCGALTNFCDAGSKKYYWFYFYLFMGLGVLAKGILAFVLPLF
                     IIFSYFLVTKNLKEMFKPMHILPGIIVFLAIILPWHTIMYLEYGNTFVKEYFLLHHFA
                     RFIDSANIGRERPFLYFVPVFLLGFLPWTLVFIAAAVRGFNKLADKFESLQGDIKSKI
                     LALTEVETNEQKLLLFAWLYFILGFLFFSFSSTKLPTYILPVFPAAALITGHFWWKND
                     EKFEDEKAIGLCTKIISAVFIITALVLSIGYFFLPPDIKAMSEHFKSIAVNGTFLLSI
                     FLLLRLDTKRSLSIFSGYVFLMLFIISLGVSCVFNFVYQFGQNELVSYASTAQDGVSQ
                     LVTFDFAVKPSVMTEYKNKVEFITDPDFEVLDEKLKFRGAPTFVIVKNKSMEDNIFAK
                     KIKSRLELITEGKKYSLYVKDVNNKYKDNTRFFAIQRIGMLDY"
     gene            12675..12866
                     /locus_tag="PKI94_05180"
     CDS             12675..12866
                     /locus_tag="PKI94_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26168.1"
                     /translation="MLTRIIQDGATHVDTLCRLMKMPSINVFWLFENAHVCIIPKRAI
                     IQDGATHVNISEVLSIFAI"
     gene            12874..14004
                     /locus_tag="PKI94_05185"
     CDS             12874..14004
                     /locus_tag="PKI94_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNW26169.1"
                     /translation="MEELVSKKVIALMSGTSCDSIDVGYCEVLPDLTCRLIEGINFKY
                     PNWVRENIFKFFQGKCDLKDVCQMNFVVGKCFADAANELIKKYGKPDFISSHGQTIFH
                     YPFDEISEGINLKSTLQIGESSAIAQETGCMTISNFREADIAQGGGGAPLVCFFDEKW
                     ARILGKNVAIQNIGGISNVTVISDRRQTFGFDNAVGNLMIDYCTSKFFNQPYDKDGKI
                     AQSGVVSESWLNCLLQDEYYICPPPKTTGREYFSPLYIENALKFAPEKPEDIIATLTA
                     LTAKAIADSYVRFVYPDVDIKEVIIGGGGAYNQTLMKFLRKYLPKHIELKTHEDYGIS
                     NNFKEVMAFAVLGYCTYYSIPNNLPSCTGAKKRVVLGKITHC"
     gene            complement(13981..14841)
                     /locus_tag="PKI94_05190"
     CDS             complement(13981..14841)
                     /locus_tag="PKI94_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNW26170.1"
                     /translation="MDYEEKEIQYLLPQRPKNSNKGTFGHLLNIAGSFKYRGAAYFSS
                     VAPLKVGCGLVTLASIEPVINSIAALTPDVIFFPLEGEKSIQNSSKILEKSLNKYQVI
                     SVGCGLSTEKGTIKFFEKLLKMFNKIDIPIIIDADGINILALLNTKPSIKNLILTPHP
                     KELARLLGITTEAIISTPDFFARKCSEEYNCTTVLKLHETIISNKQGKIHVNKTGNSA
                     LAHGGSGDVLTGIISGFLAQGMAPYEACILAVYLHGKTAEIASKKLGEYSTLASNLVD
                     YIHLAMGDFS"
     gene            complement(14831..15286)
                     /locus_tag="PKI94_05195"
     CDS             complement(14831..15286)
                     /locus_tag="PKI94_05195"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNW26171.1"
                     /translation="MKKFNIVLFEPEIPQNTGNIARLCACTGAELFLVGKLGFSLSDK
                     YTKRAGLDYWDNVVLHKFDTLEQLKEKYNNATFYYLTTKSKKSFFEAKFKEGDFLVFG
                     PETRGLPVEILEKNNKNALTIPMVEGQRSLNLSNSVSIVLYEAIRQNGL"
     gene            complement(15288..15881)
                     /locus_tag="PKI94_05200"
     CDS             complement(15288..15881)
                     /locus_tag="PKI94_05200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNW26172.1"
                     /translation="MANFISIFRIFLVFWAIYLLFQYDFYGYLWALLLTIIAFILDGV
                     DGWVARKLNETSKLGAVIDIMGDRIVENTFWVSFAVLGWLPLIFPLIALTRSFVTDSL
                     RSVAMEKGFTAFGECSMQSSKFGHFICSSKFSRISYAVSKVLAFILLIAANIPYLKFE
                     TAIKIEYVALTLAFIAIEFCVLRGLPVIFESKKFFER"
     gene            complement(15958..16956)
                     /locus_tag="PKI94_05205"
     CDS             complement(15958..16956)
                     /locus_tag="PKI94_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNW26173.1"
                     /translation="MQQQKIAVIGCGLWGRNIVRNFYNLNVLDTVCDIDEENLNKVQQ
                     DYEGVKISRDFNEILKNPEITAVAVVTPSHTHYKIVRELLLAGKHVYVEKPISTVAQE
                     AIELSKLANERGLVLMVGHLLLYHPAVNRLKMLVEEGALGEIVYAQSDRLNVNYFKND
                     RSVMWDLAPHDVSMISYVIGKEPLKVLSAFGCSSDRNEIMDITHIGIELEGGTIAHIS
                     DSWITPKKHVTLLVRGTKATAILDDTVSENKLQVFDNLAGGASKNIQLDYLEIEPLKL
                     ECQHFVQCCETGKKARSDGENGVSVVKILEEAEKIMLGEKVEKLNEVDFALSRLKK"
     gene            complement(17023..17805)
                     /locus_tag="PKI94_05210"
     CDS             complement(17023..17805)
                     /locus_tag="PKI94_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26174.1"
                     /translation="MFFEPIKEVVHVFYNRDVIIDQVSVDFSFLYATFFLLLVAWGLK
                     FFAEFIENTQKKYDEIHDFFKEKNEQVLNAQLDLENKLSEKKKKSFLILASFQAKNLK
                     KDSFFDRNVDVGVEDKEKIALNLFCSNFAQSINCQQKKTGKNVLMYFSDFEDINKVIA
                     NFEKVFEKLKTEFKEQNWSIDFTAGIEAYLEQSEINEKVNLLEKLIRLNLKNEILCLS
                     GFKQRYLVINKPEYEFSCKGIYDINGHQEVFCLINKNKIRRI"
     gene            complement(17937..18893)
                     /gene="panC"
                     /locus_tag="PKI94_05215"
     CDS             complement(17937..18893)
                     /gene="panC"
                     /locus_tag="PKI94_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNW26175.1"
                     /translation="MLEKEISQVREKISKWKKEGLKVGFVPTMGALHEGHLSLIKKSV
                     QTCDKTVVSIFVNPAQFGPCEDLDKYPKTLMEDLELCKKAHVDLVFAPSVEEMYPQEN
                     KISNDFFTYVIPPYFYVDKLCGKTRTGHFDGVCTVVTKLFNIIQPDFAFFGQKDFQQL
                     VIIKKMVQDLNIPTKIVSCPIIRDESGLALSSRNKYLSQEGKKQALVLNKILNNIRAC
                     YNKGIKDINALKETAFEFLNEHHELEYLEFVDKETLKEKPTADDNTVVLIAAKVDKMR
                     GVNDSQSNVNIQNNSVRGVNDSQSNVNIQNNSVRLIDNCFIN"
     gene            complement(18887..19741)
                     /gene="panB"
                     /locus_tag="PKI94_05220"
     CDS             complement(18887..19741)
                     /gene="panB"
                     /locus_tag="PKI94_05220"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNW26176.1"
                     /translation="MKKISVNTIKNLKKDGQKITALTAYDYSTAKYLDESGIDIIMVG
                     DSLAMVALGYETTHSIGMEEMLIFTKAVARGSKRAMVIADMPFMSYHTDLATAVKNTG
                     EMIRAGAKAVKIEGYRDYILEVIKRCTQSGIPVMGHLGFTPQFLNTIGGYNIQGKTFD
                     KTLEILEQAKQLEKAGAFAIVLEMVPEESAKYITDNLNVPTIGIGAGRYCSGQILVSD
                     DIFGKFCDFTPKFARKYGELSLLIKNCATQYIKDVQSGNFPSKEEVFKLSEEEQKQLE
                     IHSGTYKC"
     gene            complement(19738..21102)
                     /locus_tag="PKI94_05225"
     CDS             complement(19738..21102)
                     /locus_tag="PKI94_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNW26177.1"
                     /translation="MKNFLSKDIKDIKMPKDAVWLLPGLQVKRWFAMIFFGSLLILLG
                     TLIIFNMRPVFYTMEFIRKIATTINSALIGVIVALIGVIVFFKGWQKTNLSILDLHNG
                     KETGTILEALYRRRKLNRGPKIVAVGGGTGLSMLLKGIKRITNNITAIVTVGDDGGSS
                     GRLREEMGVLPPGDIRNCIAALADDEDLVTKLFQYRFKTGEGLEGHSFGNLFLSALCS
                     ITGDMVRAVKESSNVLSIRGRVLPSTLDDMKLVAEMEDGRIIRGESNIPEAHGKIKRL
                     FTDPENCRALDDAILAIKEADLIILGPGSLYTSVIPNLLINEIAQEIILSNAKKIYVC
                     NIMTQPGETDDYSVSTHINAIVSHAGNKKIMDAVLVNDSLPQNLAGKYEAANSFPVKL
                     DAENVKKSGIKIVSKRLIEENKEGLVRHSSYRVARAIYHWYRKQQKNEKGSLFSNNKE
                     VQNV"
     gene            complement(21120..21935)
                     /locus_tag="PKI94_05230"
     CDS             complement(21120..21935)
                     /locus_tag="PKI94_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HNW26178.1"
                     /translation="MQKVLDRTKKQNNEIKKITSIDFNCDLAQGFGIYRNDKEFDLLD
                     WVSSVNISTGFHAGDPLNIKNALLKAKEKNIVIGAHIGFRDIEGFGHRMMNLSEEEIE
                     ATVVYQVGAIMSFAKAYGLEVEHVRPHGAMYKLAGENFSFACAVARAIKKCSQWLVYY
                     GAAGDITEKVGEFIKIPVAHEIRLDRNYNVNGSIDYSTDGIINTEFSIRRLQNLLKAS
                     QIDNNKNGQTVIKADTIHFSSRVANSIELLKRANEIIEPIPVNYNKVKDSGWV"
BASE COUNT         6696 a         3906 c         3872 g         7567 t
ORIGIN      
        1 ccgcctccta atattaccgt atcaaattca taagttttca tcttttttcc tctcacttct
       61 caccgcttat tttatgcttg aacctgctat tttttctcca actagtttat aagtggctct
      121 ttcgcttttt ataactgttt tgtctacatc ggagacggtt tccagcaaaa gagtattaac
      181 tccttcaaag ctgtctttac ccaagtttaa ttctactata tccgtcaact ttttaccatc
      241 aaaatcgccg attttcttaa attttattaa tctgtcgctt acctcgttaa tttctactga
      301 tgccttcgct cctgaaaaag ggttttctaa tgttaagacg ttaccttctc ttgataagtt
      361 ccacaaatca accgtcgaag gtttgaatgt tgcctgactg tttgttgaag ttcttgtcga
      421 acttacccgc catgttccgt agagcatgtt gggaactctg tcaataacag aaatccctgt
      481 tttaagcgtg taagtttgtt cggcaaaaac catttgtacc gaaaacgccg ttaaaacaat
      541 tataagtgca gtaagtcttt taaacatttt ttcttccctg ataatttatt aattattctt
      601 tcggggattt caaattcttg cgggataatt tctcttaatt tttctaaagt gcagatttta
      661 aaccttttga gggcttctgt gagtttgggg taatatttat tgttgtcaag gtttttctta
      721 tatttattgt acagatagaa aagttcgtat atttccactt tttggttatc ctgacaagaa
      781 tttgtaattt gtgcctgtga aattcctata tttactaaaa ctttatttat aaaagcaatt
      841 ttgttgtttc tgatcaaagc ttctatgtac aaatccaaat ccacaagcca ttttaagttt
      901 tcatcaaaga gcaggtcgtt tttatttctg aaaattacta cgctgggaga acctattctg
      961 ttgcacaggt aaagttcaaa aggatttttc tctattttgg aaacgtattt ttcggctttt
     1021 ctggttttaa ttttttttgt ttcaacgtta aaatcgacac tttttgaata cccgaaatca
     1081 cattgaggat ttttatccat taattctaca aattttttta aagaattttc atccgcaaac
     1141 cagtcatcgt ggtggagaat ttttacatat tcgccttgag cttttttaat gccctcattc
     1201 cagtttttgg gcgcacccat tttttgggga ttcttaaaat attttattct ctcatcattt
     1261 aaaagagccg tatatatttt tatatcgtca ttttccgaat catcggttat gataatttca
     1321 aaatcactaa aagtttgaca caaaacagaa ttaagcgttc gcttaaaact ttccagatta
     1381 ttataagtgg gtattgtaac tgaaactttt attttgaaac cttccatata aatactatat
     1441 tacaaaaata tataaattga cttacttttt atttttaaat attattttat catgaacaaa
     1501 aaagtttctg taatcaccgc cagttataac tatgcggagt tcctaaaaga ggctgtcgaa
     1561 agtgttatcg cccagactta tacaaactgg gagttaattg tcgtggacga cggctctacc
     1621 gataattcgg tagaagttat caaggaattt tgcgcaagag atgagcgtat aaaatttttc
     1681 agacaccttg aaggcgaaaa cagaggactt aaagaaactc ttattttagg aatcgaaaaa
     1741 gcaaccggcg attatattgc ttttttggaa agcgatgata tctgggagca gaattgtctt
     1801 gaagaaaaga ttaaagtggc agcgcaatat cctgaggcaa aaataatttt cagtgacgtt
     1861 gaccttttcg gaaacgaaga aataatcgaa gattacgcaa gatattttga acttaacgac
     1921 agaattttgt cctcaaaaaa atatcctgca aatcttttca gtgctttcag ttttcaaaat
     1981 gtgattccga ctttttcttg tgcagtggtt gaagccgaaa cacttaagaa agcagacttt
     2041 aaccctaccg ttgaggcttt tttagaccat tgggtatggg cgcatttagc ttacgataac
     2101 aaattttatt atataaatca aaggcttacc cgctggagga tgcatacaac aagtcttgtg
     2161 ggaagacgcc aaaacaaaaa cgaaaaacaa tttttatccg atattctaaa atcttttcaa
     2221 aaaaaaggat ttaataagaa tatgtttttt tggaacaggc acaggcatcc taaaattgaa
     2281 aaaattttta gaggtttgat gagaagattt gtttaacctt tttaatgccc gattggttta
     2341 ctttttattg tcaattaatt gtgctattat gtaaaaaaat cagataaagg ggtttgggat
     2401 gaagaaattt ttagtattac tgtttgtttt ttgtttaggt gtcagtgctt ttgctcagga
     2461 ttaccaaatt tataaaatgg aaagcggaca gactgttatt ataaaacagg ttaaaaacaa
     2521 tcctattgta accattgata catggataaa aacaggttca attaacgaaa atgataaaaa
     2581 caacggcgtt tcgcattttc ttgaacatct ttttttcaaa gggacaaaaa atcatcccac
     2641 gggtgaaatg gatagacttt tggagtcaaa aggggcggtt caaaatgctg ccaccagcaa
     2701 agattttaca cattattaca taacaattcc ttctaaatac tttgaccttg cattggactt
     2761 gcactctgat atgcttttaa atccgcttat ccccagaaaa gaacttgaaa aagaacgtaa
     2821 agtcgttatg gaagaaattg ccaaagatga aaatgaccct tcaaataagg tttatgaaaa
     2881 tcttatccaa atgctttata caacacaccc ttacaaaaga aaagtcttgg gtacaaatga
     2941 cgttataggc aaaattaccc gtgaagaaat tttaaattac tataaacagt attacaatcc
     3001 tcaaaatatg gtaacaatgg taatcggaga tgttgagcca gaggatgtcc ttaaaaaaat
     3061 cagccaggag tttaaacaaa aagaagtaaa aaaatcttca aaaatttcaa ataaaaaaga
     3121 aaagcagttg tgccaaaaaa ttgtaaaagt cgattatcag ccggtacaaa gcggttatat
     3181 gcttatcggt tatagagccg cagatgcaat gaatttggat tcttatgcgc tcgatttgct
     3241 tggaataata ttgggtgacg ggcgttcaag tattttgtat cagtcaataa aagaacaaaa
     3301 gcagctggct tattctattg ctgcggaaaa ttcaaccttc aaagaagacg gaatttttat
     3361 gataagcgca aattttactg cggataaatg cgaaaaactt caaagagctg tttttgatga
     3421 aatcttaaaa attcaaaaaa acggagttac acccgaagaa ctttcaaaag caaaaagcgc
     3481 acttgaaata gaaacttatt atgcaagaga atctatttct aatattgctt cttcaatagg
     3541 ctacacaaca gttttaaccg ataacccgaa atattacgaa gagtatttag aaaatattaa
     3601 aaaagtcagc cttgctgata taaaaagagt cgcaaatatt tatttggggg agaatcaatg
     3661 tgccgtttcc atagttcttc cagaacaaga agaacaggca aaagctcaag cagcaaaaac
     3721 cgaacataat attaagatga taaatcagac atctggcgtt caaaagtacg aaatagatac
     3781 cggtgcaatt cttttgatta cacctaacga tttaaacgat attgtcgcca taagcattta
     3841 ctgcaaaggc ggcgggtttg ttgaaaaaat caaaggaact gcttttttga caagtgtcgt
     3901 tatgcttaaa ggtactaaaa attattcttc tcttgaactt gctaaaatta tggaagaaaa
     3961 cgggattaaa attcagccca atcctaccac agattctttt acaataaatg ttttgactac
     4021 aaagccaaag cttcaaaaaa cattggaaat tcttgatgaa gtggttaata gagcatcttt
     4081 tgacgattat gaaatagaaa aaacaaaaac tgcaaaactc aattcaatta aacaatctag
     4141 agatgtgcct ttaaatgttg cactcgaaga gtataaaaca cttatttacg aaaattctcc
     4201 ttacagtaca agccctaaaa ttcttgaaaa aaatcttcct aaaatccaaa gagaagatat
     4261 tttgcaatac tacagccaag tctttaatcc taaaaacatt gtgatttcag taaacggaaa
     4321 agcagagtta agcgatgtag caaaccaatt aggagatatt ttcggcacaa aagcgggtga
     4381 aaaattcagc tatgaaagtt acacctcaaa aattccgccg atttcgggta caaaaacttc
     4441 aacaaaaaat attaaagact tgcaaaccgc ttggcttgta atcggctggc agacaagcgg
     4501 gctccaaaat caaaaagact atgtcacact tcaggttata gatgccattt tgggctcggg
     4561 catgagttca agactcttta aaaatctcag agagcaagaa ggtcttgcct atcagcttgg
     4621 ctcttcttat tcgccaaacg ttttgagggg tacatttacc atgtttatcg gaacaaaccc
     4681 caaaaccctt gcgctttcac aaaaaatgct tcttgccgaa attaacaaac ttaaaactca
     4741 atttgtaggc gataaagaat taagcgaggc aaaagaaaaa atcataggaa attatcttct
     4801 ttctcttgaa acgaactcgg aaaaagcatc aaatataggc tggtttgaag cttcaggcag
     4861 agggtttgaa ttcaaagaca aatacgaaag tttaattaac agcgtaagtg cttctgatat
     4921 tgtcgaagtc gcaaacaaat actttaatca ggattatgtt gtttcggtgg ttaattcaaa
     4981 ttaaattata tgcgaaaatg ataagaaata gtcgcttatt atcgtaacaa gcataggcaa
     5041 aatccaaact aaaatcgcgg cacctaaaac gggcttaaac aagaagctaa gttgaaatac
     5101 gtttacctgc ggtgcggttt ttgaaattat ccccaaaatt atgtcctgtc ccaaagttgc
     5161 cagaagtacc ggagaagcca cttgaagccc catgtataaa acgtttgagg tcatctgcga
     5221 aagatattcc atgtttacaa tttttgtaag cggaatttgt gttgcataaa tcggaaacag
     5281 ttcaaagctt ctcataaatg cctgaaaaag ccaataaatt ccgccaagct gaataaaaat
     5341 caaaactccc aaaagcgaaa aagctttttc taaaattgaa acctgactct gtgttgtagg
     5401 gtctaaaacc atagcagaag aaagccccat ttgcatattt atcatatccg caccggcatc
     5461 tattgcaatt aatataagtt gcgccatata acctatcatc gcacctaccg cgatatttaa
     5521 aacgatagaa agaaaaagag agttacttgc aggcggtgca gaaggtttta aaactattgt
     5581 catcattata gtcaacaaaa gtgcaagtgc gatttttaca agcgtgggaa tttcttttct
     5641 gttgaacaca ggacaaaatc ttatgaatcc catcatcctt gcaaatacta taatccctgc
     5701 tgcgatagcg gagttgaaag cagggaataa ttttgcaaat tggcttaaaa taagctccat
     5761 tactcactcc tttcaaattt tatagggtgt tcgtcaagtt taatttcatc tataacaggc
     5821 ggggtgtcta acaaaagcaa ttcaaaaatt tcattttgaa gccggggtaa ttcctcatat
     5881 ttaacagagg gttttactgt tatctcaaat tttacatccc ctttgcctgt aaaaatagtc
     5941 aggtttgatt tgcccatttt taaaggctgt acaagtataa cgtttttttc gttaaaaatt
     6001 gtgaaaaaag ggcttacatt aacaatttca gaatcagaaa ccgcaatggt ttctacaata
     6061 ctttgcgtgc ttaaaatata actttttcca agatgaattt caaaactttc aagcgcaaaa
     6121 cagtttgtgc caaataataa aaatattgtt aataaaatta tcttttttac cattcaccgt
     6181 ttaccgtttc ctatttacta tttatttcct tacctgccga ggattttttt ggtttccaca
     6241 aaattaggtc tgggttgagt cgattttgga gccggagaat atttgaattt ttcctgccgg
     6301 ttttcaaatt gtgatttgcc tgatttattg attaaatcct ggatgtcggg attgtctttg
     6361 attgtatcgt tcatttcgcc tgcaaatggt cttgtgtatt tgtaataatc ggaaggcaaa
     6421 acatagctgt cggatttggg aacaacattg aaggcaacgg caacgccttc tgtaaaaaaa
     6481 tttgtcaaaa gtcttacaaa gcctattccg ccgattatta ttacaagaaa aacgcctaaa
     6541 attttcgggg cagcggcaat tgtctgttct tgaacctgag taaccgcttg caaaattccc
     6601 acaacaagac ctattcctgc tgccacaagc acgcagggca tggaaatcaa aagcattatt
     6661 acaaaacctc ttgccatgta ttcgattaat aattccattt tttaagtcct ttgtttaagt
     6721 gaataagtaa ataagttaat aagtgaatag gtgcattttt aataaattta attttttgtt
     6781 ttttacttct atttactatt tactttgttg taaatccgaa accctatgtt ccgggatgac
     6841 acggctctaa ggctcgttcc tcgccaagag ccgtgtcaat tgtaactgtt cacaagccct
     6901 tgtactatca gcgaccagcc atcgaccgcg atgaatataa ggagtttgaa cggtagtgat
     6961 ataatcgtcg gtgacagcat aaacatcccc agagcaagca gaatgtttgc aaccaccaag
     7021 tcaagtacga taaacggcac gaatattata aatccgattt caaacgccgt ttttaattca
     7081 cttatgataa aagcaggggc tacttgccag attgtgagtt cttcgggaga ttttggcggg
     7141 tttttctggg aaagagaaac aaaaaactct aaatccgatt ctctggtctg cttcatcata
     7201 aattctttta aaggttttga accttgttct attgcaagaa cgatatttcc gtttttattg
     7261 tatgggacgc ttcccaattc atacattttt tggaaaacgg gtcccattgt ataaaaagtt
     7321 aaaatcatag aaagccctat tattacaatt cctggcggaa cctgttgagt accgagtgcg
     7381 gttttgagca ttgaaaaaac aatggtaaac ctcaaaaaac ttgtcataca actgaataca
     7441 aaaggcagta acgaaaaaac agtcattact ataagcattt gtataacagg attcatatct
     7501 tttaaaactg tatccattaa ttacttcctt gcattgcttt tagcttttcg gctaattctt
     7561 tcataacagg acccttactt tttcttgctt ttttaaagtc gataatagat gccatatccc
     7621 ttatgctttc gactccgtca aaggtcggaa gctcgctgct tctgtcttgt cttatattag
     7681 gctctgttgt taaggatttt tcttccaagg gcgaaattaa agcggttctg tcaatgtctg
     7741 atgccaccaa aaacctttgt tcgcctgcgc gtattacaaa aagggttttt cttgcagaaa
     7801 gattgagtga atcttctata tttaaaaatc ctgatttttt tgaaaaagac cctgtgcctg
     7861 agacaaattt gaacacaaaa agtgctataa agatgactcc tatcatagcc attgtgtata
     7921 ttgaaaaatt aactaaatat cctgtcatat taaaccctta tcttgttgct tttatattta
     7981 atgataattt tttatacatc aaacatcctg ttcatccaat tcaaagtcgc tgtaatcaaa
     8041 ttcttcttct gcaccttctt gttcatggac ttgtgcctca gtagcacctt gttcggtaat
     8101 tacttcggca tttgtctgag aaatttcctg ctgcgcctgt gctttttgtg tggcaaaaac
     8161 ttcgtctatt ttgaccccgt acctgtcatt aattatgaca agctcacctt ttgctatggt
     8221 tttgtcttca actttaagca aaattttgtt gtcataaacc gaacttatat ccacaacaag
     8281 cccctgctca atgcttttaa gctcgcctag cgttattttt accgaatcaa atatggcatc
     8341 catttcaacc tgaatgttat cccaaagctc ttgagataaa gcgttttcgt tcatattatg
     8401 tcctccgttg atattttcta aagatgttat taatcccggg ttgggagtaa ctctgaagtt
     8461 ttgtttataa tctttgtaaa taagttccat ttgctgaata ttgctgtctt caaagattac
     8521 caaatcttct ttttcaagat tttttaattc tttaacgtaa aattttgttg ttccgacttt
     8581 tatttttact ttaacaagac tttttgaaaa atccgaaacg ttaaagtttt cttgaagatt
     8641 ttgtgcctct tgaggggtaa gaagcgtttg gggaattgaa attatgtatt tgccgaagtc
     8701 actgccgtct ttgcttttga aaaagaaagt aaggtgtgta atgtcgtggg attggtttga
     8761 attttgagga gaaaaaaatt gggaaatgtt tttgtatgca tgttcgttga atgcggttat
     8821 tatttttgct tcaagctcgg ttaaagccgt cattgaaaaa gctttttcgc ttggacctaa
     8881 aactttgttt aaaatgcttt ttacacctgt ttccgaaatt cttaaaaaca cttcgttttg
     8941 tttgttaaaa cgaactttag taacaaaata atcttcacct ttgaataaga tattttggtt
     9001 tttgctcacg gaaacaagct tgtaatcgaa taattctgag aaaaagttat cgcttgattc
     9061 ttgaattttt ggcgaaaaag tagaagaaag ccaattatat tgagcatata attcgttttt
     9121 atcggttaat ttggtatccg tataagtgtt gttcattaaa aaagcatccc cgccctgttt
     9181 ttagtatgga aaccatactc ttccccaaga ctaggataat gctttaaggc aaatcgcaca
     9241 tcaactgttt gttgtatatt ttactgtgca tgtctaagct gggtgtatat ttgaacatct
     9301 tttttatttt tttgaacaat ttcgataatt ttaaaattgt gaggatattc aacaagtgcc
     9361 ggagtgctta tgtgataaat tccgtctttt ttaatctctc cgttggcatg ataatgccct
     9421 gaaacaatcg ctataacgtt atggtgtttt tttaaaactg cttgataatc ttggacttta
     9481 tatgttgagt gcgtgcggtt ataataaggt gcttccacgg gaaaatgctg caaaataatt
     9541 acattctcat tttcatactg cgttaatttt ttatcaagcc agcttaaagt agctttttta
     9601 tagtaacctg aaggacccgg tattacttct tttgccccgt caacaacaat aaaagtaaat
     9661 ccgtttttct tgaaaacata attcgcaccc caaggtttgc aacagcggga gtgtctgcgg
     9721 acaattttca tgtaatcttt ttttgtaaaa ttttgagatt taaaaacttc gtggtttccg
     9781 attacaatat agtaaggcac tttgagttta tttgcctttt gcaaaaatgc ttttaagtca
     9841 atttcgttgg ctgtatcaat attatcacct gtaaatacca caaaatctac atttttaatt
     9901 gagtttatgt cttgaagggt gttatccaaa aatttttccg aattttcaac ttctctttgc
     9961 gtatattcgg tcgaattttt tgaaaagtgt gaatctgtta tttgaataaa tttaatattt
    10021 tgtgcataag aataatttcc aaaagcagtc atgataataa aaacagttaa aaacagatat
    10081 gttaactttt tcataatatt tgcaaaaatc ctcccgttat ggtaaacttc cttcgtaatt
    10141 gtaccataaa aagaagtttt attttaataa gaaggagaat atatgaaaga ctttttacat
    10201 aatgcctccg gtggtttgga aaaaaatgtt tttgctaaag tggtaaattt ttttaaaggg
    10261 atgataaata atcatcccga gatgtttgct gttttatttt tattttttat gtgtttgctt
    10321 tttttatttt tcgggctgaa tttttatcct ttaattgatg ttgatgaaac aagatatgcg
    10381 gttatcgcaa aaaatttaat aggctcaagg gactggaaca gtttgatact taactatgaa
    10441 ccgtttttgg aaaaacctcc tttgtatttt tggcttgttg ccgcttcggt aaaattcttt
    10501 ggcggattga gtgcttttgc cataaggttt ccaatggcaa ttttggcatc ttttttaacc
    10561 tttttcacat attttttcgg aaagaaagtt atatcaagaa agtttggtat tgtaagtgcc
    10621 cttgttcttt tgaccagcat attttttctt attttatcgc atgtggcaat tcttgatatc
    10681 gtagtaaccg tttttgtggc cagtgcactt tattgcgggg ctttaacaaa tttttgtgac
    10741 gcgggatcca aaaaatatta ttggttctat ttttatcttt ttatggggct gggggtttta
    10801 gcaaaaggga ttcttgcctt tgtacttccg ctttttatta tattttctta ctttttggtt
    10861 acaaaaaatt taaaagaaat gtttaagccg atgcatattt tgcccggtat tattgttttc
    10921 ttggcaatta ttttgccctg gcatacaatt atgtatttgg aatacggaaa tacttttgta
    10981 aaagaatact tccttttgca tcactttgca aggtttatag attcggcaaa tataggcaga
    11041 gaaagaccgt ttttgtattt tgtccctgtg tttttgttgg gatttttgcc ttggactctt
    11101 gtttttattg cagcagcagt tcgcgggttt aataaacttg ccgacaaatt cgagtctttg
    11161 caaggggaca ttaaatcaaa aatattggct ttaacagaag ttgaaaccaa tgaacaaaaa
    11221 ttgcttttgt ttgcctggct ttatttcata ttaggatttt tatttttctc tttttccagc
    11281 acaaaattac ctacttatat cttgccggtt tttcctgcgg cagctttgat tacaggacac
    11341 ttctggtgga aaaatgacga aaaatttgaa gacgaaaaag caatagggct ttgtacaaaa
    11401 attatttcgg cggtatttat tataaccgca ttggttttga gcataggata ttttttcctt
    11461 ccgccggata tcaaagccat gagcgaacat tttaagtcga tagcggtaaa tggaactttt
    11521 cttttaagta ttttccttct tctaaggctt gatacaaaaa ggtctttatc aatattttca
    11581 gggtacgtgt ttcttatgtt gttcattata tccttgggtg tttcgtgtgt ctttaatttt
    11641 gtttatcaat tcggacaaaa tgagcttgta agctacgctt caaccgctca agacggagtg
    11701 tctcagctgg ttacttttga ttttgcggtt aaaccaagtg ttatgactga atataaaaat
    11761 aaagtcgaat ttattacgga tcctgatttt gaggtgcttg atgaaaaact caagtttagg
    11821 ggggcaccta cttttgtaat cgttaagaat aaaagtatgg aagataatat ctttgcaaag
    11881 aagataaaat caaggcttga attgataacg gaaggaaaaa aatactcctt atatgtcaag
    11941 gatgtcaata ataaatataa agataatacg aggttttttg ctattcaaag aataggcatg
    12001 ctcgattatt aatcttttga attttttata cggggaattt ttgttgtcaa caaaaattcc
    12061 cctatttttt taccaaaatt cattaaattt tcttcattaa gattgtaaaa tctaattttt
    12121 ttcaacaggg ataaggggca tggtcaattg taaaaattat tgaaaaattt gttacttgta
    12181 catgcccttt gaaggtttaa cataataata aagggaccga agaaaaggcg gatagcaata
    12241 cgttggtttc ggggtatgta ttagaacaag gaagaatgaa ttagaaaaag gggtgatggc
    12301 taactggact ttgcgaggag cggggaaaat aataaatctg agagaggatg ggattattgc
    12361 tccgtaaaaa agaaaaaggt tactggattt ggggtttgag ttttatttta gggaaatgaa
    12421 aaaaagtttt aacgagcaaa gttagatgaa gtaatgaaaa agattaattg aatgcgttta
    12481 tagtaaatgc tagtactgaa taattaggag tttataaaaa tctgatagtt gtagattaac
    12541 ttgcctcatg gcaagttttt tttgcggatt ttcgggaggg cgtcgtgtgg ctttagccac
    12601 ccagcccgta agagcaaata gtccgatgga gtgctaaaat tgcgaaagca attttaaaaa
    12661 cggaattaag gactatgctc acccgaataa tccaagacgg tgctacgcac gtagatacac
    12721 tgtgtagatt aatgaaaatg ccttcgatta atgttttttg gctttttgag aatgcgcatg
    12781 tgtgtataat cccaaaacgc gcaataatcc aagacggtgc tacacacgta aacataagcg
    12841 aagtattatc tatttttgcg atataatcaa cttatggaag aattggtttc aaaaaaggtt
    12901 atagcactga tgtcagggac ttcgtgcgac tccattgatg ttggatactg cgaggttttg
    12961 cccgatttga cctgcaggtt aattgaaggt attaatttta aatatcctaa ttgggtaaga
    13021 gaaaatattt ttaaattttt tcagggcaaa tgtgatttaa aagatgtttg tcagatgaat
    13081 tttgttgtgg ggaaatgttt tgccgatgcg gcaaacgagt taattaaaaa atacggcaaa
    13141 cccgatttta tttcttcgca cgggcagaca atttttcact atccttttga tgaaataagt
    13201 gaagggataa atttaaaaag cacactgcag attggcgaat cctctgctat cgctcaagaa
    13261 accggatgca tgacaatttc aaatttcaga gaagccgata tagcacaagg cggagggggt
    13321 gcgccgttag tttgtttttt tgatgaaaaa tgggcaagaa ttttgggtaa aaatgtcgct
    13381 atacaaaata tcggcggcat ttcaaatgtt acggtgatat ccgacaggcg gcaaaccttc
    13441 ggctttgaca atgcggtggg gaatcttatg atagattact gcacaagcaa attcttcaat
    13501 cagccttacg acaaggacgg taaaatcgca caaagcgggg ttgtaagtga gagttggctg
    13561 aattgtcttt tacaagatga atattacatt tgtccccctc ccaaaacaac aggaagggag
    13621 tatttttcgc ctctttacat tgaaaatgct ctaaaatttg caccggaaaa acccgaagac
    13681 attatcgcta ccctcacagc attgacggca aaagcgatag ccgattctta tgtaagattt
    13741 gtctatcctg atgttgatat aaaagaggtt ataataggcg gcggcggggc ttataatcaa
    13801 actttgatga agtttttgag aaaatatctg ccaaaacaca ttgaacttaa aacgcacgaa
    13861 gattacggaa tttctaataa ctttaaagaa gttatggctt ttgcggtttt gggttactgc
    13921 acttactatt caattccgaa caacctgcct tcttgcacag gtgctaaaaa gagagtggtt
    13981 ttaggaaaaa tcacccattg ctaagtgaat ataatcaaca agattagatg ccagagtaga
    14041 atattctccc agttttttgc ttgcaatttc tgctgttttt ccgtgcagat agacggcaag
    14101 aatacaagcc tcataaggtg ccatgccttg agcgagaaac cctgatatta tccccgtcaa
    14161 aacatcaccg cttcccccgt gagccagtgc ggaattgccg gttttattga cgtggatttt
    14221 tccctgtttg tttgagatta ttgtttcatg gagttttaaa accgttgtgc agttgtattc
    14281 ttctgaacat tttcttgcaa aaaaatcagg cgttgaaatt atcgcctcgg tagttatccc
    14341 taaaagtctt gcaagctctt ttgggtgagg ggttaaaatg agatttttga ttgaaggttt
    14401 tgtattgagc aatgctaaaa tatttattcc gtcggcgtca attattatgg gaatatcaat
    14461 tttgttgaac attttgagta atttttcaaa gaattttatt gtgccttttt cggtcgaaag
    14521 cccgcagccc acagatatta cctgatattt gtttaaagat ttttctaaaa ttttggaaga
    14581 attttggata gatttttcgc cttcaagcgg gaaaaagatt acatcgggcg tgagtgctgc
    14641 aatcgaattt atcacaggct ctatgcttgc caaagtaacc agcccgcagc cgacttttaa
    14701 gggcgcaaca cttgaaaaat aagctgcccc acgatattta aaagagccgg cgatatttag
    14761 cagatgcccg aaagttcctt tgttggaatt tttgggcctt tgcggaagca ggtattgaat
    14821 ctctttttct tcataatcca ttttgtctta tcgcctcgta taaaactatt gaaaccgagt
    14881 ttgataaatt taaacttctt tgtccttcaa ccattggaat tgtcaaggcg tttttgttgt
    14941 ttttttctaa aatttccacg ggcaaacctc ttgtttcagg accgaataca agaaaatcac
    15001 cttctttgaa tttcgcttca aagaaagatt ttttgctttt tgttgtcaga taataaaaag
    15061 tagcattgtt gtatttttct ttcaattgct ctaaagtatc gaatttgtgt aaaacaacat
    15121 tgtcccaata atctagtccg gcgcgttttg tgtatttatc agaaagtgaa aaccccaact
    15181 ttccgactaa aaaaagttcg gctccggtgc acgcacacag ccttgcaatg tttcccgtgt
    15241 tttgtgggat ttcaggctca aacaatacaa tgttaaactt tttcatatta tctttcaaaa
    15301 aactttttgc tctcaaatat gaccggcaag cctcttaaaa cacaaaattc tattgcaata
    15361 aaagccaaag tcagtgcaac atattctatt tttattgcgg tttcaaattt taagtaagga
    15421 atgtttgccg caatcaaaag aataaaagcc aaaacttttg aaacggcata agagattctt
    15481 gaaaactttg agctgcaaat aaaatgacca aatttgcttg attgcatgct gcattcgcca
    15541 aaagccgtaa accctttttc catagctaca cttcttaaac tgtcggtcac aaagcttctt
    15601 gtaagtgcaa tgagcggaaa aatcaggggc agccagccca aaacggcaaa agacacccag
    15661 aaagtatttt ccacgatcct gtcgcccata atgtctataa cagcacccaa ttttgaggtt
    15721 tcattgagtt ttcttgccac ccagccgtca accccgtcta atataaaggc gataattgta
    15781 agcaaaagtg cccacaaata cccgtaaaaa tcatattgaa aaagcaggta tattgcccaa
    15841 aagaccaaaa atattctgaa aatacttata aaatttgcca taatttactc cttaattttg
    15901 aaaatccatg ctttaggatg acgcagctct aaggcttatt cttagccaag agccgcgtca
    15961 tttctttaac ctcgaaagcg caaaatcaac ttcattaagt ttttcgactt tttctccgag
    16021 catgattttt tctgcttctt caagaatttt aactacgcta acgccgtttt cgccgtcaga
    16081 gcgcgctttt ttacctgttt cgcagcattg aacaaagtgc tggcactcaa gtttaagagg
    16141 ctctatttca agataatcaa gctgaatatt ctttgatgct ccgcctgcga ggttatcaaa
    16201 tacttgcagt ttgttttcag aaacagtatc atctaaaatt gccgttgctt ttgttcctct
    16261 taccaaaaga gtcacatgtt tttttggtgt aatccaactg tctgagatgt gtgcgattgt
    16321 tccgccttca agctctattc cgatgtgggt tatgtccata atctcgtttc tgtctgatga
    16381 gcatccgaag gcagaaagca cttttaaagg ttcttttcct ataacataac ttatcattga
    16441 aacatcatga ggagctaaat cccacattac gcttctgtca tttttaaagt aatttacgtt
    16501 taatctgtcg ctttgggcat agacaatttc tcctaaagca ccttcttcaa caagcatttt
    16561 aagtctgttc accgcagggt ggtacaaaag caaatgtcct accattaaga ccaaacccct
    16621 ttcattggca agctttgaaa gttctattgc ttcttgtgca acggttgaaa tgggtttttc
    16681 tacgtaaaca tgttttcctg caagaagaag ttctcttact attttgtagt gggtgtgact
    16741 tggtgttaca acagcgacgg cagttatttc cggatttttt aaaatttcgt taaagtcgcg
    16801 gcttattttt acgccctcat aatcttgttg aactttgttt aaattttctt cgtctatgtc
    16861 gcagacagta tctaaaacat taagattata aaaatttctt acaatgtttc ttccccaaag
    16921 tccgcatccg atgactgcta ttttttgttg ttgcatattt tctcccgctt ttttcttaat
    16981 tataatataa ttttattttt tttcctaatt atgaatataa ctttaaattc ttctgatttt
    17041 atttttgttt ataagacaga aaacttcctg atgaccgtta atatcgtata tccccttgca
    17101 tgaaaattcg tattcgggtt tgttgattac cagatatctc tgtttgaaac cgcttaaaca
    17161 aaggatttcg ttttttaaat ttaaccttat taatttttcc aaaaggttta ctttttcgtt
    17221 aatttcggat tgttccaagt aggcttcaat accggcagtg aaatctattg accaattttg
    17281 ttctttaaac tctgttttta atttttcaaa aactttttca aaatttgcaa ttactttatt
    17341 tatgtcctca aaatcgctga aatacattaa aacatttttg cctgtttttt tttgttggca
    17401 atttattgat tgtgcaaaat tggagcaaaa taaattcagg gctatttttt ctttatcttc
    17461 tacccccaca tcgacgtttc tgtcaaaaaa gctgtctttt ttgaggtttt ttgcctgaaa
    17521 agaggcaagg attaaaaaac tttttttctt tttttcggat aatttatttt ctaaatccag
    17581 ttgggcatta agtacttgtt cgtttttttc tttaaagaaa tcgtgtattt cgtcatattt
    17641 tttttgagta ttttcaataa attcggcaaa aaatttcaaa ccccatgcca caagcagaag
    17701 gaaaaaagtt gcataaagga aagaaaaatc tacactcact tggtcaatga taacatctct
    17761 gttataaaag acatggacaa cttcttttat cggttcaaaa aacattgcaa aattttcaat
    17821 aaatttgacc tgagctattt gcaaaatcca gtataaaatt atgaaaaagc accaaaaaac
    17881 taaaaatacc cgagtcattt ggataatgtt tataaatatt tcaaaaaatt ttcttcctaa
    17941 tttataaaac aattgtcaat caacctcaca ctattatttt gtatattaac atttgattga
    18001 gagtcattta cccccctcac actattattt tgtatattaa catttgattg ggagtcattt
    18061 acccccctca ttttatcaac ttttgcggca atcagtacaa cagtattatc gtctgccgtt
    18121 ggtttttctt taagagtttc tttatcaaca aattcaaggt attcaagttc gtgatgttcg
    18181 ttcaaaaatt caaaagcagt ttcttttagc gcattaatat cttttattcc tttattataa
    18241 catgccctta tgttatttaa gattttgttt agtacaagag cctgtttttt gccttcttgt
    18301 gataaatatt tgtttcttga actcagtgca agtccgcttt catcacgaat aataggacaa
    18361 gacacaattt tagtcgggat gtttaaatct tgcaccattt ttttgataat aacaagttgc
    18421 tgaaaatctt tttgaccgaa aaatgcaaaa tcgggctgaa ttatattaaa aagttttgta
    18481 acaacagtac aaaccccgtc aaaatgtcct gtacgggttt tcccgcaaag cttatcgaca
    18541 taaaaataag gcggaataac ataagtgaaa aaatcgtttg aaattttatt ttcttgcgga
    18601 tacatttctt cgacacttgg ggcaaaaacc aaatctacat gagctttttt gcataattct
    18661 aaatcttcca ttagggtttt ggggtatttg tctaaatctt cgcaaggtcc gaattgggca
    18721 ggatttacaa aaatagaaac aaccgttttg tcacatgttt ggacactttt tttaatcagg
    18781 ctcaaatgcc cttcgtgcaa cgcacccata gtcggcacaa aaccgacttt taaaccttct
    18841 tttttccatt ttgaaatttt ttctctgacc tgagaaattt ctttttctaa catttataag
    18901 tccctgaatg aatttcaagt tgtttttgtt cttcttctga aagtttaaaa acctcttctt
    18961 ttgaagggaa attgccgctt tgcacatctt taatgtactg tgtggcacag ttttttatca
    19021 aaaggctcaa ctcaccgtat ttgcgggcaa attttggagt aaaatcgcaa aacttgccaa
    19081 aaatatcgtc tgaaaccaaa atttgccctg aacaatatct gccggcaccg attcctattg
    19141 tagggacatt caggttatcg gttatgtatt ttgcgctttc ttcgggaacc atttctaaga
    19201 caatcgcaaa agcacccgcc ttttcaagct gttttgcctg ctcaaggatt tctaatgttt
    19261 tgtcaaaagt ttttccctga atattgtatc ctccgattgt atttaaaaat tgcggggtga
    19321 aacccaaatg tcccataaca ggaattcccg attgagtgca tcttttaata acttccagaa
    19381 tataatcacg atacccttct atttttaccg cttttgcgcc tgctctaatc atctccccag
    19441 tgtttttaac tgccgttgcg aggtcggtat gataactcat aaagggcatg tccgctatta
    19501 ccatagctct ttttgagcct cttgccacag cctttgtgaa aatcagcatt tcttccattc
    19561 cgatagaatg ggtggtttca tatcccaagg caaccatagc aagagaatcg cccaccatga
    19621 ttatatctat gccgctttcg tcaaggtatt tggcggtaga ataatcgtaa gctgttaggg
    19681 cggttatttt ttgaccgtct tttttaaggt ttttaattgt gtttacgctt atttttttca
    19741 tacgttttga acttctttat tgttgctgaa taaagagcct ttttcgtttt tttgctgttt
    19801 gcggtaccag tgataaattg cccttgcaac tctgtaagag ctgtgtctta caagtccttc
    19861 tttgttttct tctataagac gttttgaaac aattttgatg cctgattttt ttacgttttc
    19921 ggcgtccagt ttaacgggga atgagtttgc cgcttcgtat tttccggcaa gattttgagg
    19981 caggctgtca tttaccagaa cagcatccat gattttttta tttccggcgt ggcttactat
    20041 tgcgtttata tgtgttgaaa cactgtaatc atcagtttca ccgggctgtg tcatgatgtt
    20101 gcagacatat attttttttg cgtttgaaag aataatttct tgggctattt catttataag
    20161 caaatttgga attacgcttg tataaaggct tcccggccct aaaattatca aatccgcctc
    20221 ttttatggct aaaattgcat catcaagtgc cctgcaattt tcagggtcgg taaaaagtct
    20281 tttaattttg ccgtgcgctt cgggaatatt ggattcgcct cttataattc ttccgtcttc
    20341 catttcagca acaagcttca tatcatcaag tgttgaagga agaactctgc ctcttattga
    20401 aagaacgttg gaagattctt taaccgctct taccatatcg cctgtgatag agcaaagcgc
    20461 acttaaaaat aagtttccga aactgtgtcc ttcaagtcct tcgcctgttt taaagcggta
    20521 ttgaaaaagt tttgtaacca aatcttcgtc atcggcaagt gctgcgatac agtttcttat
    20581 gtcaccggga ggtaaaacgc ccatttcttc tcttaatctt cctgagcttc cgccgtcatc
    20641 tcctacagta actattgcgg ttatattatt tgttattctt ttaatgcctt tgagcaacat
    20701 agaaagacct gttccgccgc ctactgcaac aattttaggc cctcggttaa gtttccttct
    20761 tctgtaaagt gcttcaagaa ttgttccggt ttcttttccg ttatgtaaat ctaaaattga
    20821 caaatttgtt ttttgccagc ctttgaaaaa gacaatcaca cctatcaaag ctactataac
    20881 tcctattaat gcagaattta tggtagttgc aatttttctt ataaattcca tggtgtaaaa
    20941 tacagggcgc atgttaaata ttatcaatgt gcctaaaagg attaaaagag atccgaaaaa
    21001 tatcatcgca aaccatcttt taacctgcag ccctggcaaa agccatactg catcttttgg
    21061 catttttata tctttaatgt cttttgataa aaaatttttc acgtttcgcc tttcgttttc
    21121 tatacccagc cggagtcttt tactttgttg taatttaccg gtatcggctc gataatttcg
    21181 tttgctcttt ttaaaagttc aatagagttt gcaaccctgc ttgaaaagtg tattgtatca
    21241 gcttttataa ctgtctgtcc gtttttgttg ttgtcaattt gagaagcttt taaaagattt
    21301 tgaagccttc tgattgaaaa ttcagtattt ataattccat ctgttgaata gtctattgaa
    21361 ccgttaacat tataatttct gtcaagccgt atttcgtgtg caacaggaat tttaataaat
    21421 tcgcctactt tttcggtaat atctccggct gcaccgtaat aaacaagcca ttgggaacat
    21481 tttttaattg ctcttgcaac cgcgcaggca aaggaaaaat tttctccggc aagtttatac
    21541 attgccccat ggggccttac atgttcaact tcaagtccgt aagcttttgc aaaagacata
    21601 atcgcaccga cttgataaac aaccgttgct tcaatttctt cttcggataa attcatcatt
    21661 ctgtggccaa agccttcaat atctctaaag cctatgtgtg ctccgattac aatgtttttc
    21721 tcttttgctt ttaaaagtgc gttttttatg ttaagcggat ctccggcatg aaaacctgtt
    21781 gaaatattta cagagcttac ccaatcgagc aggtcaaatt ctttgtcatt tctgtagatt
    21841 ccaaatcctt gcgccaaatc acagttaaag tctatagaag tgattttttt tatttcgttg
    21901 ttttgttttt tagtcctgtc caaaactttt tgcatttctt ccccttatag ttttgtcacg
    21961 agcttgcgga aaaattaaaa tttttctcgc aatctccctt gtccggcttt catccttgcc
    22021 gccggctatc ccgcagccgg c
//
LOCUS       DAOQAU010000027        21763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2447_length_21763_cov_6.636171, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000027 DAOQAU010000000
VERSION     DAOQAU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 21763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21763
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2447_length_21763_cov_6.636171"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKI94_05235"
     CDS             complement(<1..506)
                     /locus_tag="PKI94_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNW26179.1"
                     /translation="MDKLNFLTAGIPLRTEPKDYENAFRVLNEMNLDGMELEFVHGVR
                     MSDESQKTVKKVSKDFVITAHGPYYINLNSREQEKIDASVVRIIDTALMANKVGAYSI
                     TYHAAFYMGNDKEVVFKRVVEQTKRIIDVIEKESAKVWIRPETTGKSTQWGDLDEVIR
                     LSKEFEQV"
     gene            complement(499..1437)
                     /locus_tag="PKI94_05240"
     CDS             complement(499..1437)
                     /locus_tag="PKI94_05240"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNW26180.1"
                     /translation="MSDFKFRKRVLFIGIPDMAYVCLDGLKAAGVNIVGAFGAKKDHN
                     TYESFKYFANSRNVNFIEYDNLKDEEFIAKIKELKPDVAVVCSFNYKIPKVLLDAVPG
                     GFINIHPSLLPKYRGGNPYSPVIMNDEKVTGVTIHFMEENFDTGDIISQKQTLVTPKE
                     TMGTLFNRLNLLGLEMLLQALRDFEKGNLPRQKQPKGEFPVGNLLSDAELFIDFNKSA
                     SEIERFIRALNPFFNASTMFRNTFVKIFSADATDFDAVSQSPVGTIEKIKDDKFFIKT
                     SSGLISPKVLQFGSFFVSDSKDFIKILNLKEGEKFG"
     gene            complement(1430..1801)
                     /gene="crcB"
                     /locus_tag="PKI94_05245"
     CDS             complement(1430..1801)
                     /gene="crcB"
                     /locus_tag="PKI94_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNW26181.1"
                     /translation="MINIIAVFLGGGLGSLARYFMSILLRVYSMDFPFATLAVNMLGC
                     FILGLVVALFWENTNIHEGFKLALTVGFCGGFTTFSTFSGEVFALIKQGDYMLAFIYV
                     IISVIICLLATILGAFLARYV"
     gene            complement(1885..2286)
                     /locus_tag="PKI94_05250"
     CDS             complement(1885..2286)
                     /locus_tag="PKI94_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNW26182.1"
                     /translation="MKNKASDYYKQGNSCSESIIKAAVDAGLCDESLLSVASAFSGGM
                     SSGCLCGAVAGSQMVLGHNFGKRNSKNNQEIARQKAKEFIEEFKKNRKATCCKVLTAG
                     LDPVQRKQNCTSLVEECAQILEKMIKLNTSS"
     gene            complement(2321..3052)
                     /locus_tag="PKI94_05255"
     CDS             complement(2321..3052)
                     /locus_tag="PKI94_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNW26183.1"
                     /translation="MFFSVLFMLNNAVFAIQSTINPEMLTISDSINYEKINSLSTKLF
                     KNIETSINTDIYPYKYDNFPLLEKKWYQSTEYYYYVSCEDVELIFNKKTKDLEYISFP
                     QKGYPKCFIIYEYPSGNLKMVKIGFSKNEIYIFNSNGKFVDFKTYVDDVNQKIKSNWI
                     APKNVKNTGLIVLLTINRKGQLENYKIVQPSGSKDFDDSGIRAILQSVPFKPIPESYP
                     IKTIYINFIFIVEDARYKFPYQNNR"
     gene            complement(3104..4186)
                     /gene="mtnA"
                     /locus_tag="PKI94_05260"
     CDS             complement(3104..4186)
                     /gene="mtnA"
                     /locus_tag="PKI94_05260"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNW26184.1"
                     /translation="MAGFESKIKTIEWADNCSRMVDQTVVPYEFKYVDIKTGQQMYDA
                     IQTMVVRGAPAIGIAGAHGVALYAQELAGKNLKREEFISKLIEGSKYLASSRPTAVNL
                     RWAIEKQLELVKSCRCEEEQSDDEAIQKIVKKLIQNGVKLENEDIEINKKIGDFGAQV
                     VPKGATILTHCNAGALATVGYGTALGVVRSAYATDNTIQVFADETRPRQQGARITTFE
                     LVMDKIPVTLITDGMCSYFMKKGMIDMVVVGADRIAANGDTANKIGTYTVAIAANYHN
                     IPFYIAAPLSTIDISIKSGEEIPIEERDHEEVTHINGKSICAEGTRVINPAFDVTPNE
                     LITGIITEVGILKTDYKISIAKAFKK"
     gene            complement(4201..4686)
                     /locus_tag="PKI94_05265"
     CDS             complement(4201..4686)
                     /locus_tag="PKI94_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26185.1"
                     /translation="MNPLLMKQTAILSAILGGILGVLTLIPFVRNFSFMILILCISAV
                     VIVYMKRHELIGIIDLREGALLGAIIGFVSFIAFSVVFIPLVTIVGWIFKGYYTYGIS
                     YMLKVSGFFVLVMLIIFLAILSALMNAFAGLVTAYVYEVLLGIKKEEKEKESIEFEIK
                     E"
     gene            complement(4698..5189)
                     /gene="ruvC"
                     /locus_tag="PKI94_05270"
     CDS             complement(4698..5189)
                     /gene="ruvC"
                     /locus_tag="PKI94_05270"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNW26186.1"
                     /translation="MKILGIDPGMAIVGYCVIEFDGRNVELLNSGSIKTSKNNTESQR
                     LLEIFEDINTVIQRYNPDIASVEKLFFFKNHKTVMPVAAARGVIMMALEKCGVEVFEY
                     TPMEVKQTLTGYGRADKKEVEQMVKIAFNGQTLPKLDDTIDSMAIAICHARNYNCKKL
                     LKV"
     gene            5631..7349
                     /locus_tag="PKI94_05275"
     CDS             5631..7349
                     /locus_tag="PKI94_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNW26187.1"
                     /translation="MSVGQFVFMLHSHLPYYRKAGMWPFGEENLYECMAETYIPLLNA
                     IAELYDEGIKAKLTVGITPILAEQLNDDHLQQGFIKFLDNRIEAVAKDLKRYPDPNVA
                     HSQHLKYLAKYYFDWFNHIKDTYVNRYKMDLIGTFKKYQDLGCIEITTSGATHGFSPL
                     FETDTNLNAQFKIGSDTTKRLFGQQPCGAWLPECAYRQGYEYVGKDGEKKWRPAIEVI
                     LQNNDIEYFFTESHVIEGGNSIGNRRVIGVYGNIEYIPLPEREATGYDTYSAYWLPDA
                     QVAVMGRNDRAGYQVWSAADGYPGDGCFREFHKKDDKSGAHYWRITSPTTDLGDKMLY
                     DPVLALNKVNENSDHYTTLIYHLLNDYKLATGKEGLVMVSFDTELFGHWWFEGVEFIK
                     QVIKKFSTYVPEVERCTACEYIREHPPTEAIQIPESSWGQGGHFWVWQNHLTEWMWPI
                     IHSCEKRIMEVVSKYEQIPQDKLLFRALNQLARENLLLQSSDWPFLITTWQAKDYATD
                     RFREHVERFEKIADMIENNSIDEDYLKSVESIDNCFEVIDYRVYLPIEEGKIPQQEAK
                     LAPLYQ"
     gene            complement(7467..8138)
                     /locus_tag="PKI94_05280"
     CDS             complement(7467..8138)
                     /locus_tag="PKI94_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26188.1"
                     /translation="MKITSKNFSNNIQVSFQSRNRELRLAETVMRKVNNEFPRVSPDY
                     LNRIFVRHNRISSAWLNAKKSLLCQYRQILAQSCNDPEVLVSYVKNAKVGNCGESAIL
                     SKMALDLNGVDRVKIFNLTEFIKNGENLTLKDVDHSIVVVNMDKSAILNDYKTFGKKA
                     YVVDSWMGIVGYVSDVFEQYMKIFNLRNNLGVFPKSNTNSDLSELAQIFAPNLKIRKE
                     ELKFI"
     gene            complement(8189..10339)
                     /locus_tag="PKI94_05285"
     CDS             complement(8189..10339)
                     /locus_tag="PKI94_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNW26189.1"
                     /translation="MKKKVIAYLHTHWDREWYREFEVFRLRLLRVFDNVLALLEKGKI
                     PSFYFDGQTAALEDYLELNPEKEKLVRKLIEQKKLFIGPFYTLVDEFLTDQRCFKKNL
                     EIGLKYAKNLGCKDFLGYFADTFGHSQNIPTILEEFGIDKAVVWRGCPDEIPSEFIFN
                     GVKTVNLVRGYFNDIFSTNLSIKEKGDFIQSHLDKIAQKSSDVLLMPIGADHLGLESD
                     ILEQIKEVNKLLKDYEIVLSSPFEYFRLVKDNFKFKYEGELRNNSQTFILQGCYSARM
                     KLKQYNVKCSYMLNLANKFQKFAQGKYKTKSYDNIIEYAYKLLLQNQAHDGICGCSTD
                     KVHAENIIRYEKILQIAETIIEELKFKTGEPSLIVNLSTVNFEGVVQFESCVKLPEND
                     CQIIGIRKGFSSDFLQNIHKIPVTEDYTNIYTYLTEVECKEPSKLLSKPHKLYPKIFY
                     PLDLAVSDTKLENDNISLTIKNGKIIVEDKKTDKIYKDFIKFVDFKDKGDSYNFGPVI
                     NDKGLESIIISSKILRKGKLQCALLVKFKIGTTILNTEISLNKGSKLLNFKILWNNKI
                     KNHLLQLSFNLLEPIKETYSEDMNFLIRREFDPDYDIRKNLPKTKGIEAKTNTAPMQR
                     YVWAQGFEVVTKGLCEYEVKKNFLLITLLRAIGMISNPKNPARTTPAGPPLEVFDSQQ
                     LGENIAEFAIGFGDKENWHDLVEEIYPKIIINKI"
     gene            complement(10391..11506)
                     /locus_tag="PKI94_05290"
     CDS             complement(10391..11506)
                     /locus_tag="PKI94_05290"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNW26190.1"
                     /translation="MNVEQETKMQIGIPRAMSFYTNYPFLYGFLTELGIEIVLSDKTT
                     KKTLAKGSSLVVSETCLPVKVYVGHILNLIDKGVDKIFVPSFQSIAPKIYNCSKIRGL
                     PDLIRNVVKKDFQMIEATLDKSEKNQGFYEFLKEIVGPFGITDEKRIKKASKAGWKVY
                     NNFKIMSRSGMSYKQAMNYALQGKVFISQETREYPISVALVSHPYNIYDEQISMRIFD
                     KLNDMDVKVYSANELTPEQMQEGINALNQRLYWANEYEMTGCAGHYLKDNKIDGIINL
                     TAFGCGPDSLMIERINRKAKKFNKPILNLTIDEHTGEAGFVTRLEAFVDMLYRKKRAK
                     IINKIKLSEDEYIPNTNFIKIKETEKSQDLIFDGDFV"
     gene            11898..13967
                     /locus_tag="PKI94_05295"
     CDS             11898..13967
                     /locus_tag="PKI94_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNW26191.1"
                     /translation="MTIKKLTMAAAIAVGIATCSINTAMAACPCSSTPSCASPPCACA
                     TPCNSCCDKCKCNPCKCNPCDCDPCKKSCDPCCDPCAKPATPACATCPNTSCADLDMK
                     QVYAYPNAIYGDNNYVGEQKNAIYSTETAWAFDNCDSLALSRAGVSVVGCGQMTGAAV
                     PLTCDGMPIITGAAAPCCGCNSMPLIGPSACGCGCLNNGAIVDRNCCKMDNSCCPIQI
                     QTSNSIDALKKSFVPFEPNIAGTQCSCGCTGAAAPLVDAFPDVPSNYWAACPIDKLAM
                     NNVVVGYPDRLFRPCKDVSRAEFATMIVKGYNLNCSSLPTKNIFKDVPRSHWANSLIA
                     KAVDEGIMCGYPGCKFKPNNHVSRVEALTAMAHSINCEMDSCKAQQILSQYCDGDKVP
                     SWAQIPVAKALETGILKNSLTPNTINPCKDASRAEIADMLQTTRIAMGLDTNPATACD
                     CPKPADPCCPKKAYMEQEEIVKIPTLKLEFLDEINAKSSHVGQQFATTTLEEVTINGH
                     CYPCGSRVNGKIVEVIRPSGCEKGALKLAFTTIEDCSGCCADLPRQILTAQINCAKTQ
                     NAVARLVSMPFTWAGALVGTTGRTIGGMISNAGNAFESVSNGVGIASGEIFQGQFRAS
                     GRSLQDAGKAAIVAPVDFARTAFSGATGLFQTTGDEVAYLVDPKGYKVAAVNPKEHLT
                     IAFGCSD"
     gene            14118..14423
                     /locus_tag="PKI94_05300"
     CDS             14118..14423
                     /locus_tag="PKI94_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNW26192.1"
                     /translation="MNKKVYSLEEIKNKIKENEKFLKEKYHVRGFLLFGSYAKGEQTQ
                     DSDIDLLVDFQKVIDMFEMIDLQEYLQNLFGKKIDLGTKNGLKSFIKKTILKEAINL"
     gene            14420..14770
                     /locus_tag="PKI94_05305"
     CDS             14420..14770
                     /locus_tag="PKI94_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNW26193.1"
                     /translation="MKQRNPLFYLKDIQTSVNKILKYTENMSFEVFKKDEKTIDAVER
                     NFEIIGEAVKNLSEEIKSKYPDVPFKQIAGMRDKLIHDYFGVDYEIIYKTIKDKIPEF
                     KFKIEKIIKSINKN"
     gene            14867..15613
                     /locus_tag="PKI94_05310"
     CDS             14867..15613
                     /locus_tag="PKI94_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26194.1"
                     /translation="MKVHACPDKYKYPKNLPMQQKYRKFSNFSNINFSSKDILVKSTP
                     VQKTQDFSWTNDPVILKSLDYLKNLNFDTENIKYVQAQGAILPFKSGAQAFEFINKSN
                     TRIKFDTLSMPNTHAQYDFENNTIKINEIYKNTQATEEILAISEAILHETGHAKDGDG
                     QNSVQEEINCLALNSVAHRDFVAKYSDIFQNSNAPIIKDGVIIYESLFFDFDPQKNNL
                     ITRLKEKYGFLPAGDFTHPPSELAFRVKRN"
     gene            15822..15983
                     /locus_tag="PKI94_05315"
     CDS             15822..15983
                     /locus_tag="PKI94_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26195.1"
                     /translation="MQTVATNDVKIKKSKSRFNDEFESYLKKQCLKTTFNGLEIETFS
                     WYKKVLGLL"
     gene            complement(16211..17497)
                     /locus_tag="PKI94_05320"
     CDS             complement(16211..17497)
                     /locus_tag="PKI94_05320"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNW26196.1"
                     /translation="MDTLVLTPKKLTRLIVHTVNFCVVLGVLVAQHVFEGPPLIAQEQ
                     IKLANNTAISPIFNTVTLEANVAKQLSIEQKTKAFDEEIKRKYKTGIITEVDKGVKHI
                     RITKYYQGQPVRINVIEVNSEINPDLQLTPAIASETLANKASISSIAKKNNSIVAING
                     TFFKPATGVPLGTLMINKKMYTGPIYNRVAMGIFDNKYDMARVQLDATLKSGLHSVKV
                     DNINQPRMLSTYVIVYTREWGKFAPPSPKYGKQIAVKDNKVIEVSTSALAIPEDGFVI
                     VAPEARLKDLLGAKNIRFDVATLPEWQGVNHIISGGPYLVKHGEVFVDMTEQKLASIG
                     GRNPRSAIGYTADNNLILVTVDGRESSSVGMTLMELANFMKSIGCINAMNLDGGGSTV
                     LYVNGQVVNQPQVKGGIALSNALTLNKTNSEIGYKD"
     gene            complement(17910..19193)
                     /locus_tag="PKI94_05325"
     CDS             complement(17910..19193)
                     /locus_tag="PKI94_05325"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNW26197.1"
                     /translation="MKNTVVVGAQWGDEGKAKITDLLATKADLIIRYQGGCNAGHTVV
                     VEDKTYKFHLIPSGILYEGKTCFIGAGTVIHPETFEQEVKDLIKQNISPKGLKVSPLA
                     SITMPYHIEMDGYSEDKAKKGKIGTTKKGIGPTYTDKVARWGLKIEDLYSFEALSEKL
                     DVILPIKNKMLKTFGLKTYTKQEITQLCAKYAKIFESYVDFDWQNTLNEVKRVNKTIL
                     FEGAQGVMLDIDYGTYPFVTSSNPIAGGACTGSGLGPTSIKEIIGVSKAYLTRVGEGP
                     FITELEDEVGGKIRDIGREFGTTTGRPRRCGWFDVLIAKYAVLVSGLTSMAITKLDVF
                     DDFDEIKICTAYKDKRDGRIYKNYPTNVFVHKYLEPIYETHKGWKKHISNCRKWEELP
                     LNAQNYLKRLEELFEIPISIVSVGPKREQTILLDE"
     gene            complement(19216..20226)
                     /locus_tag="PKI94_05330"
     CDS             complement(19216..20226)
                     /locus_tag="PKI94_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNW26198.1"
                     /translation="MENEKENKGILIYAEITREGTLAPVVLELANTAYDLAQKLGGEE
                     ISAVLITKTGNIEYIKNELRNNGFDKVYIIQDDRLRDYSTELYSKIAIDLVRQVKPSI
                     FLIGATTQGRDIAPRISSTLNTGLTADCTGLDINEKGQLAATRPTFGGNLMATILCKN
                     LPQMASVRPKVLKKASEPRVKDTQFIDFPSNIDGIDKKVEIVEFIKNFQAASGPRIDE
                     AEIIVAGGKGLKSPDGFKLLEELAQVLGAAVGASRPCVDAGWADHAIQVGQTGKTVMP
                     KLYIACAISGAIQHTVGMSGSEKIIAINKDPKAPIFGVADIGIVGDVYEVVPELIKML
                     KG"
     gene            complement(20262..21056)
                     /locus_tag="PKI94_05335"
     CDS             complement(20262..21056)
                     /locus_tag="PKI94_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNW26199.1"
                     /translation="MKVALCIKQVPDTADIKWTENNTIQREGVESVINPFDMYAIETA
                     VRLKENFKDVHITVLSMGPPQAQDILKRAISMGVDEGFLVSDKKFAGADTVATSRTLA
                     TAIKEKVKDVDLIICGQFATDGDTAQTGPSIAQQLNFPQVTYVKEIVDYKDGFLTVKR
                     EVEDGIEIIRTTLPAVLCMLKCDYEPRRPLINGFVKAQNAQINMYNAEDIKLQPEQVG
                     IKGSPTYVSRAFRPEQKQGGDVITPETPQKGAEIIKEKLKEFEIVK"
     gene            complement(21080..21256)
                     /locus_tag="PKI94_05340"
     CDS             complement(21080..21256)
                     /locus_tag="PKI94_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26200.1"
                     /translation="MSEKNIPYCPLMSAGNTIEIICTQERCAWYLKNYRMCAMYVLAH
                     NAALDIQSKQPKKN"
     gene            complement(21260..>21763)
                     /locus_tag="PKI94_05345"
     CDS             complement(21260..>21763)
                     /locus_tag="PKI94_05345"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNW26201.1"
                     /translation="CYLIAKAFKEAGNKVYMIMGARNKELIFWQEKMEKACSELFITT
                     DDGTLGEKGFVTQVLERIMNSEKVDYAIAVGPMPMMRAVANMTRDKGIYTEASMNPIM
                     VDGTGMCGACRVTVGGEVKFACVDGPDFDAHKIDFDEVINRMKIYKEQEQKHDENCKL
                     FKQKVKE"
BASE COUNT         6652 a         4422 c         3668 g         7021 t
ORIGIN      
        1 aaaacctgct caaactcttt tgaaagcctt attacctcgt ctaaatcgcc ccactgggtg
       61 ctttttcctg tggtttcagg gcgaatccaa acctttgcgc tttccttctc aataacatca
      121 ataattcttt ttgtctgttc gacgactctt ttaaaaacga cttctttgtc gtttcccata
      181 taaaacgctg cgtgatacgt tatgctgtaa gccccgactt tatttgccat aagtgctgtg
      241 tcaataattc ttacgacact tgcatcgatt ttttcttgtt ctctggaatt aaggttaata
      301 taataaggac cgtgagctgt tatcacaaaa tcttttgaca ctttttttac ggttttttgg
      361 ctttcatcgc tcatcctgac accgtgaaca aattcaagct ccattccgtc aaggttcatt
      421 tcatttaaga ctctgaaagc attttcatag tctttaggct ctgtccttaa agggattccg
      481 gctgtgagaa aattcagttt atccaaattt ttctccttct ttaaggttta atattttgat
      541 aaaatctttt gaatcggaaa caaaaaaact tccgaactgc aaaactttgg gcgaaataag
      601 cccgcttgaa gttttaatga aaaatttatc atcttttatt ttttcgatag ttccgacagg
      661 actttgcgat actgcatcaa aatctgttgc atcggcggaa aatattttga caaaagtatt
      721 tctgaacata gtagaagcgt taaaaaacgg atttagagcc cttataaaac gttcaatttc
      781 tgaggcggac ttattaaaat caataaaaag ttctgcatct gaaagcaaat tcccgacggg
      841 aaattccccc ttaggctgtt tttgtctggg caaatttcct ttttcaaaat cgcgcaaagc
      901 ctgcaaaagc atctctaagc ccaaaaggtt taacctgtta aaaagagtcc ccatagtttc
      961 tttaggagta acgagagttt gcttttgaga aattatgtca cccgtatcaa aattttcttc
     1021 cataaaatga atggtaacgc ctgtaacttt ttcgtcgttc attattaccg gcgaataagg
     1081 gttgccgcct ctgtatttgg gcaaaagcga aggatgaatg ttaataaaac cgcccggcac
     1141 agcatcgagc aaaacttttg ggattttgta gttaaaagaa caaacaaccg caacatcggg
     1201 ttttaactcc ttaatttttg cgataaattc ttcatctttt aaattatcgt attcaataaa
     1261 attcacattt ctgctgtttg caaaatattt aaaagattca taagtattat ggtctttttt
     1321 tgcgccaaaa gcacctacga tattaacccc tgcagcctta agcccgtcaa gacaaacata
     1381 agccatatcg ggaattccga taaataaaac tctttttcta aatttaaaat cagacatacc
     1441 ttgctaaaaa tgcccccaga attgttgcca aaaggcagat aattacactt attatcacat
     1501 aaataaaagc aagcatataa tcgccttgct taattagggc aaaaacttcg cccgaaaaag
     1561 tgctgaatgt tgtaaatccg ccgcaaaaac cgaccgtaag agccaattta aagccttcgt
     1621 gaatattagt attttcccaa aaaagcgcaa ccacaagccc caagataaaa catccaagca
     1681 tattcacagc taaagtcgca aacgggaagt ccatactgta aactctaagc aaaatgctca
     1741 taaaataacg ggctaaagaa ccgagcccac cgcctaaaaa aacagcaata atattaatca
     1801 tttttacctc tcacttatta tgtcatgctg aatttatttc agcatcttac cagcttactg
     1861 cgctgaatag atcctgaaac aagttcagga tgacgtattt agttttatca tcttttctag
     1921 tatctgagca cattcttcta ccaaagaagt gcaattttgt tttctttgga cagggtcaag
     1981 ccccgcggtt aaaaccttac aacaggttgc cttcctgttt tttttgaatt cttcgataaa
     2041 ttcttttgct ttttggcggg caatttcttg gttgtttttt gaatttctct tgccgaaatt
     2101 atgccccaaa accatttgag aacctgcaac agcaccgcaa agacagcctg atgacattcc
     2161 gcctgaaaat gcgcttgcaa cagacaacag gctctcatcg caaagccctg cgtcaactgc
     2221 cgcttttata atactttccg agcaggaatt tccctgtttg taataatctg acgctttatt
     2281 tttcattaat tccgtccttt tcaaaaatta taccatacaa ttatcgatta ttttggtagg
     2341 gaaatttata acgtgcatct tcaacgataa aaataaaatt aatatatata gttttaatgg
     2401 ggtaactttc aggaattggc ttaaacggta ctgactgcaa aatagctcta attccgctat
     2461 catcaaaatc ttttgagccg gatggttgca ctattttata attctcaagt tgccctttcc
     2521 tattaatagt taaaagtact atcaatcctg tatttttaac atttttcgga gcaatccaat
     2581 tcgattttat tttttgattt acatcatcaa cgtatgtttt aaaatcaaca aatttgccgt
     2641 ttgaattaaa aatataaatt tcatttttag aaaaccctat tttgaccatt tttaagttgc
     2701 ctgatggata ttcataaata ataaaacact ttggataacc tttttgagga aaagaaatat
     2761 attctaaatc tttcgttttt ttattaaaaa ttaattcaac atcttcgcaa ctcacataat
     2821 aataatattc ggtactttga taccattttt tttcaagaag cggaaaatta tcgtatttat
     2881 atgggtaaat atctgtgttt attgaagttt caatattctt aaaaagttta gtacttaaac
     2941 tattaatttt ttcatagttt atagaatcac ttatggtcaa catttccgga ttaatagtac
     3001 tttgtatagc aaaaacggca ttattcaaca taaataaaac tgaaaaaaac attatcaaat
     3061 acttcataaa actactcttt tcttattaat tgtttgtcga atgttatttt ttaaaagctt
     3121 ttgcaataga aattttataa tctgttttta agattccgac ttctgttata attcctgtta
     3181 taagctcatt tggagttacg tcaaaagcgg gatttataac tcttgtccct tcggcacaaa
     3241 tgcttttgcc gtttatatgt gtaacttctt catggtcgcg ctcttctatg gggatttctt
     3301 ctcccgattt tatgcttatg tctattgtgg ataaaggtgc agcaatataa aaagggatat
     3361 tatggtaatt tgcggcaata gcaaccgtgt aagtccctat tttatttgct gtatcgccat
     3421 ttgcggcaat tctgtcggca ccaaccacaa ccatatcaat cattcccttt ttcataaaat
     3481 aagagcacat tccgtctgtt ataagagtta cgggaatttt gtccataaca agttcaaaag
     3541 tggtaatcct tgccccctgt tggcgcggtc ttgtttcatc agcaaaaacc tgaattgtgt
     3601 tgtctgtggc ataagctgag cgaacaacgc ccagagcagt accgtagcct acggtggcaa
     3661 gcgcgcctgc attacagtgg gttaaaattg ttgcgccttt tggcacgacc tgcgccccga
     3721 aatcacctat ttttttattt atttcaatgt cttcgttttc aagtttgaca ccgttttgaa
     3781 taagtttttt aacaattttt tggattgctt cgtcatcgct ttgctcttcc tcgcaacgac
     3841 atgattttac aagttcaagc tgtttttcaa tcgcccagcg taaattaacc gccgtagggc
     3901 gagagcttgc aagatatttt gaaccttcaa ttaattttga aataaattct tctcttttta
     3961 gatttttgcc cgccaattcc tgtgcgtaca aagcaacccc atgggcgcct gcaatcccga
     4021 ttgcaggcgc cccgcgcact accatcgtct gaatcgcatc atacatttgc tgacctgttt
     4081 taatgtcaac atacttaaac tcatacggca caaccgtctg gtcaaccata cgggaacaat
     4141 tatccgccca ttctattgtc tttatttttg attcaaatcc tgccatttaa ccctttaccc
     4201 ctactcttta atctcaaatt ctatgctttc tttttctttt tcttcttttt taattccgag
     4261 cagaacttca taaacataag ccgttacaag ccctgcaaat gcgttcatta aagcacttaa
     4321 aatcgcaagg aaaattataa gcattactag gacaaaaaat cccgaaactt tcagcatata
     4381 agaaattccg taagtgtaat agcccttaaa aatccagccg acaatcgtta caagcggaat
     4441 aaacacaacc gaaaaagcta taaacgaaac aaatccgata atcgccccta aaagtgcgcc
     4501 ttctctcaaa tcgattattc cgataagttc gtgtcttttc atatagacaa taaccaccgc
     4561 cgagatacac aggattaaaa tcataaacga aaagtttctt acaaagggaa ttaaggttaa
     4621 aacccctaaa attccgccta aaatcgcact taaaatcgca gtttgtttca ttaataatgg
     4681 attcattttt tcctccgtta aacttttaaa agttttttac aattatagtt tctggcatgg
     4741 caaattgcaa tcgccatgga atctattgta tcatcaagtt ttggcaaagt ctgtccgtta
     4801 aaagcaattt taaccatttg ttcgacttct tttttgtccg ccctgccata gcctgttaaa
     4861 gtttgcttta cttccatagg agtgtattca aaaacttcaa ccccgcattt ttccagcgcc
     4921 atcataataa caccccttgc cgcagcaaca ggcataacgg ttttgtggtt tttaaagaaa
     4981 aaaagctttt ccacagaggc aatatcagga ttataccttt gaataacagt atttatatct
     5041 tcaaaaattt ccaaaagacg ctgggattcg gtattatttt tagaagtttt aatcgagcct
     5101 gaattaagca actcaacatt tctgccgtca aattcaatga cacagtagcc gactatcgcc
     5161 attcccggat ctattcccaa aattttcata ttccaattat aacagcaaca gcggattaaa
     5221 caaaaactta atattagagc atgcgggaaa gtccgatttt cggaaaatcg agactttccc
     5281 gcatgtgacc tcagaagtgt aaaaaccctg ccagctgcgg attagccggt ggcagggttt
     5341 gaaaccggac ataggagttt acaggaaaaa ggaacttttt tcgtaaactc attaagttaa
     5401 attcccactc aacttttctt taacatttgt aacattattc taaagaatta gtccaaaagg
     5461 attataattt ttaaccaatt gtgctatata aatgttatac aagattgtag gataattaat
     5521 aagtgaataa gtgaataggt taatgagtaa ataagaaatt ttttgcgaag caaaatttta
     5581 acctttcagc ctttcaccca ttcaccccat caaccaaaaa ggaggcagaa atgtcagtag
     5641 gacaatttgt atttatgtta cacagccacc tcccctatta cagaaaagcc ggaatgtggc
     5701 ctttcgggga agaaaactta tatgagtgta tggctgaaac ttatatcccg ctattgaacg
     5761 caatagcaga actttatgat gaaggaatca aggcaaaatt aaccgtcgga atcacaccga
     5821 ttcttgccga acagttaaat gacgaccacc ttcagcaagg cttcataaaa tttttagaca
     5881 acagaataga agcggtagcc aaggatttga aaagatatcc cgaccctaac gttgctcatt
     5941 cccagcactt aaaatacctt gcaaaatact attttgactg gtttaaccac atcaaagaca
     6001 cttatgttaa cagatacaaa atggatttga taggaacttt caaaaaatat caggacctgg
     6061 gttgtataga aataacaact tcaggtgcta cacacgggtt ttcacctttg tttgaaacag
     6121 acacaaacct gaacgcacag ttcaaaatcg gttcagacac aacaaaaaga cttttcggtc
     6181 aacagccttg cggagcttgg ctgcctgaat gtgcttacag acaaggttat gaatacgtag
     6241 gaaaagacgg agaaaagaaa tggcgtcccg cgattgaagt tattcttcaa aacaacgata
     6301 ttgaatactt ttttaccgaa tctcacgtaa tcgaaggcgg aaattcaatc ggaaaccgca
     6361 gagttatagg ggtttacgga aatattgaat acattccttt gcctgaaaga gaagcgacag
     6421 gctacgacac atattcagct tactggcttc cggatgcaca agttgcggtt atgggaagaa
     6481 acgacagagc aggatatcag gtttggtcag ctgccgacgg atacccgggc gacggatgtt
     6541 tcagagaatt ccacaaaaaa gacgataagt ccggtgctca ctactggaga ataacttctc
     6601 cgacaaccga tttgggcgac aaaatgctct atgatcctgt gcttgcgctg aataaagtta
     6661 atgaaaattc agaccactac acaactttaa tttaccactt gctaaatgac tacaaacttg
     6721 caacaggtaa agaaggactt gtaatggttt cgtttgatac ggaattgttc ggccactggt
     6781 ggtttgaagg tgttgaattc attaaacagg ttatcaagaa gttttcaact tacgttccag
     6841 aagtcgaaag atgcaccgct tgtgaataca taagagaaca tcctccgaca gaggcaattc
     6901 aaattcctga aagctcatgg ggacagggcg gacacttctg ggtatggcaa aaccatttga
     6961 cagaatggat gtggcctatt atacactctt gcgaaaaaag aattatggaa gttgtttcca
     7021 aatacgaaca aattcctcaa gacaaacttc ttttcagagc tctcaaccag cttgcaagag
     7081 aaaacctgct tctgcaaagt tcagactggc cgtttttgat tacaacatgg caggctaagg
     7141 actatgcgac agacagattc agagaacacg tcgaaagatt tgaaaaaatc gccgatatga
     7201 ttgaaaacaa ttcaatcgat gaagattacc tcaaatcggt cgaatcaatc gataactgct
     7261 ttgaagtaat cgactacaga gtctatctgc cgattgaaga aggcaaaatt cctcaacagg
     7321 aagctaaatt ggcacctctg taccaatagt gaactgtgag cagtgaacga tgaactgtta
     7381 atcggactaa caaagtccga caaaaccata ataaaatacg gcggggtttc gtaaacgaaa
     7441 tcccgccgta ttttattaaa aacaatttaa ataaatttca actcctcttt tcttattttt
     7501 aaattcggag caaatatttg agccaattca ctcaaatcgc tgttggtatt agacttagga
     7561 aaaacaccca aattatttcg taaattaaaa attttcatat attgttcaaa aacatctgaa
     7621 acgtaaccta caatacccat ccaagagtca accacataag cctttttgcc aaatgtttta
     7681 taatcattca agatagcaga tttgtccata tttaccacaa cgatactatg atctacatct
     7741 ttaagtgtta ggttttcccc attttttata aattcagtta aattaaaaat ttttactcta
     7801 tccactccgt ttaaatcaag agccattttt gataaaattg cagattctcc gcaatttcct
     7861 acttttgcat ttttaacgta gctgactaaa acctccggat cattacatga ttgagctaaa
     7921 atttgtcgat attgacacaa taaacttttt tttgcattta accaagcaga actaatgcga
     7981 ttatgcctaa caaaaattcg gtttaagtaa tcgggtgaaa ctcttggaaa ttcattatta
     8041 acttttctca tgactgtttc agccaaacga agttcacgat ttcttgactg aaaagaaact
     8101 tggatgttgt ttgaaaaatt ttttgatgta attttcatat caatataaaa tcagctgaaa
     8161 attaaaattg cctgaaattt tgacattttt aaattttgtt tataattatc ttcggataaa
     8221 tttcttcaac caagtcgtgc cagttttctt tatcaccgaa tcctattgca aattctgcta
     8281 tattttcgcc aagctgctgc gagtcaaaaa cttcaagcgg cgggcctgca ggagttgttc
     8341 ttgcagggtt tttaggatta gaaatcatac cgatagctcg aagcagagtt attaaaagaa
     8401 aattcttttt aacctcatac tcgcaaagcc cttttgtaac gacttcaaac ccttgtgccc
     8461 aaacatatct ttgcataggg gcagtatttg ttttagcctc gatgcctttt gtttttggaa
     8521 gattttttct tatatcatag tcgggatcaa actcccttct gataagaaaa ttcatatcct
     8581 ccgaataagt ttcttttatc ggctcaagca ggttaaaact tagctgaagc agatgattct
     8641 taattttgtt attccaaaga attttaaaat taagcagttt tgacccctta tttagtgaaa
     8701 tttctgtatt taaaattgtt gttcctatct taaatttcac caaaagcgca cattgaagtt
     8761 tgccttttct taaaattttt gaggaaatta taatgctttc aagaccttta tcatttatca
     8821 cagggccaaa attataactg tctcctttat ctttaaagtc aacaaatttt ataaaatcct
     8881 tgtaaatctt gtctgttttt ttatcctcaa caataatttt gccgtttttt atagtcaaag
     8941 aaatattatc attttcaagt tttgtatcgg aaaccgctaa gtcaaggggg taaaatattt
     9001 tagggtataa tttatgaggt tttgaaagaa gctttgaagg ctccttgcac tcaacttcgg
     9061 ttaaatacgt gtaaatgttt gtataatctt ctgtcactgg gattttatga atattctgta
     9121 aaaagtcgct tgaaaatcct tttcttatcc cgataatttg acaatcattt tcaggcaatt
     9181 ttacacaaga ttcaaactgt acaacccctt caaaattaac tgttgaaagg tttacaatca
     9241 aagaaggttc gcctgtttta aattttaact cttcaattat tgtttctgca atttgcagaa
     9301 ttttctcgta tcgaattatg ttttctgcat gaactttatc tgttgagcac ccgcaaattc
     9361 cgtcgtgggc ctgattttgc aacaataatt tataagcata ttcgattata ttatcataag
     9421 actttgtttt gtatttaccc tgagcaaatt tttgaaattt atttgcaagg tttagcatat
     9481 aagagcattt tacattgtat tgcttaagct tcatccttgc cgaataacac ccttgcaaaa
     9541 taaatgtttg ggaattattc ctgagctcac cttcgtattt aaatttaaaa ttgtctttta
     9601 ccaacctgaa atactcaaaa ggtgatgaaa gtacaatttc ataatctttt aaaagtttat
     9661 ttacttcctt aatctgctct aaaatgtcgc tttccaaccc caaatggtct gcaccgattg
     9721 gcattaaaag gacatcggaa gatttttggg cgattttatc caaatggctt tggataaaat
     9781 cgcctttttc tttaatgctt aaattagttg agaaaatatc gttaaaatag cctcgcacga
     9841 ggtttacggt tttaactccg ttaaagataa attctgaagg gatttcatcg ggacaacctc
     9901 gccacacgac agctttatct attccaaact cttctaaaat tgtcgggatg ttttggctgt
     9961 gtccgaaagt atctgcaaaa taccccaaaa aatctttgca gcctaaattt ttagcgtatt
    10021 tcaaacctat ttcaagattt ttcttaaaac atctttggtc ggttaaaaat tcatcaacga
    10081 gagtataaaa aggtccaata aaaagttttt tttgctcaat taatttgcgc acaagttttt
    10141 ctttttcggg attcagttca aggtaatctt caagggctgc tgtttgtccg tcaaaataaa
    10201 aggaagggat tttgcctttt tccagtaagg ccaaaacatt gtcaaagact ctcaaaaggc
    10261 gaagcctgaa aacttcaaac tctctgtacc attccctgtc ccaatgagtg tgcagatatg
    10321 caataacttt tttcttcata aatgtatttt aacgcattga acaaaaaaag tgaaaaagtt
    10381 tacaaaaaag ttatacaaaa tcgccgtcaa aaattaaatc ctgagatttt tcggtttctt
    10441 tgatttttat aaaatttgta ttaggaatat attcatcttc agaaagcttg attttattaa
    10501 taatttttgc tctttttttg cggtaaagca tatcaacaaa agcttcaagc cttgttacaa
    10561 aaccggcttc gcctgtgtgt tcgtctattg ttaaatttaa aatgggttta ttaaactttt
    10621 tggcctttct gttaattctt tctatcatta aagaatccgg accgcagcca aaagccgtta
    10681 aatttataat tccgtctatt ttgttatctt taagataatg ccccgcacag cccgtcattt
    10741 cgtactcgtt tgcccagtaa agcctttgat taagcgcatt tatgccttcc tgcatctgtt
    10801 ccggtgtaag ctcatttgcg ctgtaaacct taacgtccat atcattaagc ttatcaaaaa
    10861 tcctcatgga aatttgttca tcataaatat tataagggtg cgacacaagt gcaactgaaa
    10921 tcggatattc tcttgtttcc tgtgaaataa aaacttttcc ctgaagcgca taattcatag
    10981 cctgtttata gctcataccc gagcgtgaca tgattttaaa attgttataa actttccacc
    11041 ctgcctttga tgccttttta attctttttt cgtcggttat tccaaacggt ccgacaattt
    11101 cttttaaaaa ttcgtaaaat ccttgatttt tttctgattt atcaagtgta gcttcaatca
    11161 tttgaaaatc tttttttaca acatttctta ttaaatctgg aagccctctt attttggagc
    11221 aattataaat tttaggagca atagattgaa aagaaggaac aaaaatttta tcaacacctt
    11281 tatctattaa gtttaaaata tgtcccacat agacttttac aggcaaacac gtttcgctta
    11341 caacaagtga agatcctttt gcgagggttt tttttgttgt tttatcagat aaaactattt
    11401 caatgccaag ttctgttaaa aacccgtaca aaaacggata attagtgtaa aatgacattg
    11461 ccctggggat accgatttgc atttttgtct cttgttctac attcatagta gtatcataat
    11521 ccaaacaaaa aataattgct agttaggggt aaatggtgaa tagaaatgtt aaaacagggg
    11581 acaagaggcg aagatgccaa gaggcgtagt aggataaccc acgcatccac gcatctactc
    11641 atcttatctt cttataaatt attcgactgt tcgacttttc gacttttaat cctcttcacc
    11701 cctcacccct cacctctcac ctctcaccct tcacttctca cccttcgcct ctcacttttc
    11761 aaatagccca ggcggataac cgtttttggc aatgtttcat attgtattta gaatacataa
    11821 tcatttaaca agtttgtttt ttagattaaa agcacaagat ttttgattaa atcttaaaaa
    11881 aacacaagga ggtaaaaatg acgattaaaa aacttactat ggcagctgcg attgccgtcg
    11941 gaatagcaac gtgttctata aatacggcaa tggcagcttg tccatgcagc agcactccaa
    12001 gttgtgcctc gccgccatgc gcttgtgcaa caccttgcaa ctcttgctgc gacaaatgca
    12061 aatgcaaccc gtgcaaatgt aatccgtgtg attgtgaccc atgcaaaaag tcatgcgatc
    12121 catgctgtga cccttgcgcc aaacccgcaa ctccggcatg tgcgacatgt ccaaacacaa
    12181 gttgtgctga tttggacatg aaacaagttt acgcttaccc taacgcaatt tacggcgaca
    12241 acaattacgt gggagagcaa aaaaacgcaa tttactcaac tgaaacagcc tgggcgtttg
    12301 acaactgtga ttcacttgcg ctttcaagag caggcgtatc ggtcgtcggc tgcggacaaa
    12361 tgacaggcgc agcagttcca ttaacctgcg acggaatgcc tattattaca ggagctgcag
    12421 caccttgctg cggatgtaat tcaatgccgc ttataggtcc ttctgcctgc ggatgcggat
    12481 gcttgaacaa cggtgcaata gttgacagaa actgttgcaa aatggacaat tcctgctgtc
    12541 ctattcaaat tcaaacttcc aattcaattg atgcattaaa aaaatcattc gtcccttttg
    12601 agccaaacat agcaggaact caatgcagct gcggatgtac aggtgctgct gcacctttgg
    12661 tggatgcatt tcctgatgtt ccgtcaaact actgggcagc ttgtccgata gacaaactcg
    12721 caatgaataa cgtcgttgta ggttaccctg acagactctt tagaccctgc aaagatgtat
    12781 cccgtgcaga atttgcaaca atgatagtta aaggctacaa cttgaattgc agtagcttac
    12841 ctacaaaaaa catcttcaaa gacgttccaa gaagccactg ggctaattcc ttaatcgcaa
    12901 aagcggttga tgaaggaatt atgtgcggat atcccggctg caaattcaaa cccaataacc
    12961 acgtatctcg tgtagaagca cttaccgcaa tggctcacag cattaactgt gaaatggact
    13021 cttgcaaagc tcaacaaatc ttgagccaat actgtgacgg tgacaaggtt ccaagctggg
    13081 cacaaattcc cgtcgcaaaa gctcttgaaa caggcatact taaaaacagc ttaacaccta
    13141 atacaattaa tccttgtaaa gacgcttcaa gagcagaaat cgcagatatg cttcaaacaa
    13201 caagaattgc tatggggtta gacacaaacc ctgcaacagc ctgcgattgc cctaaaccgg
    13261 cagacccttg ctgtcctaaa aaagcataca tggaacaaga agaaatcgtt aaaattccta
    13321 cacttaaatt ggaattccta gacgaaatca acgcaaaatc ttctcacgta ggtcaacagt
    13381 ttgccactac aacacttgaa gaagtaacta tcaacggaca ctgctatcct tgcggttcaa
    13441 gagttaacgg taaaatcgtt gaagttataa gaccttcagg atgcgaaaaa ggtgcactga
    13501 aacttgcttt cacaactatc gaagattgca gcggttgctg tgcggacctt ccaagacaaa
    13561 ttcttaccgc tcagattaac tgcgctaaaa cacaaaatgc ggtagcaaga cttgtttcta
    13621 tgccatttac atgggcaggt gcacttgtcg gtactacagg aagaacaatc ggcggtatga
    13681 tttcaaatgc gggtaacgct tttgaaagcg tctccaacgg agtcggtatc gcatcgggcg
    13741 aaatcttcca gggacaattc cgtgcctcag gcagaagctt gcaagatgca ggaaaagcag
    13801 caatcgttgc acctgttgac ttcgcaagaa cagccttcag cggtgctaca ggactattcc
    13861 aaaccaccgg cgacgaagtt gcttaccttg ttgaccctaa aggctacaaa gttgcagcag
    13921 ttaaccctaa agaacaccta acaatcgcct ttggctgctc agattaatat atacacttat
    13981 ccttgaaaaa ggggcggaca cgaagtgtcc gccccttttt atattcaata ccaatgtaca
    14041 aaatttttaa tatatttcaa aaaattgcta aaaagcaaaa aatatgttaa aattccaata
    14101 cttaaaagag gcaaattatg aataaaaaag tttattcttt agaagaaatt aaaaataaaa
    14161 taaaagaaaa tgagaaattt ttgaaagaaa aatatcacgt tcgaggtttt ttacttttcg
    14221 gctcatacgc aaaaggtgag caaactcaag acagcgatat tgatttgctt gtagattttc
    14281 aaaaagtcat tgacatgttt gaaatgattg atttgcagga atatttacaa aatttattcg
    14341 gtaaaaaaat tgatttagga acaaaaaatg ggttaaaaag ttttatcaaa aaaaccattt
    14401 taaaagaggc aataaactta tgaagcaaag aaatccgtta ttttatctaa aagatattca
    14461 aacatccgta aataaaatac ttaaatatac agaaaatatg tcttttgaag tatttaaaaa
    14521 agatgaaaaa acaattgatg cggttgagag aaactttgaa attataggcg aagcagtgaa
    14581 aaatttatcc gaagaaatta aaagtaaata ccccgatgtc ccttttaaac aaattgcggg
    14641 aatgagagat aaattaattc acgattattt tggagttgat tacgaaataa tttataaaac
    14701 aataaaggat aagatacccg agtttaagtt taaaatagaa aaaatcatca aatccataaa
    14761 taaaaattag aacttttgaa aaaaaaagag tcacccatct gggtgactct ttttttttca
    14821 aatttatact ataatttgaa aattcccccc aaaaggatga ttctccatga aagtacatgc
    14881 ctgccccgac aagtacaaat accctaaaaa cttaccgatg caacaaaaat atcggaagtt
    14941 tagtaatttt tcaaatataa acttttcatc caaagatatt ttagtcaagt caactcccgt
    15001 gcaaaaaaca caagattttt catggacaaa tgaccctgta atcttaaaat cacttgatta
    15061 tttaaaaaac ttaaattttg atacggaaaa cataaaatac gttcaagctc aaggtgcaat
    15121 tttgcccttt aaaagcggag cacaagcctt tgaattcata aacaaatcta atacaagaat
    15181 aaaatttgat actctttcca tgccaaacac tcacgcccaa tatgattttg aaaataacac
    15241 cattaaaata aacgaaatct ataaaaacac tcaagccacg gaagaaattc ttgcaatatc
    15301 agaagcaatc cttcatgaga caggacatgc caaagacggt gacgggcaaa attccgttca
    15361 ggaagaaatc aactgtcttg cccttaattc agttgcccac agagatttcg tagcaaaata
    15421 ttcagacatt tttcaaaact caaatgcccc tatcatcaaa gacggggtaa taatttatga
    15481 aagtttattt ttcgattttg atccgcaaaa aaataattta ataaccagac ttaaagaaaa
    15541 atacggcttt ttgcccgcag gcgactttac acatccgcca agcgagcttg cttttagagt
    15601 taaaagaaac taatatttaa aaaacaattt tgctttataa aaatttacag tatttttttg
    15661 cagtaactag caattttttt ttatttcatg tttttatacc tctagattta attcaacctt
    15721 catgttataa tagctgtgtt cccaagttca tgtttcaacg cattttctgc gtattttata
    15781 tagataatta caaataggtt aagatagtaa ggagaaaatc tatgcaaacc gtagcgacta
    15841 atgatgtaaa aatcaaaaaa tccaagtcaa gattcaatga tgaattcgag agttacctga
    15901 aaaaacaatg tttaaaaaca acatttaacg gactggaaat cgaaacattt tcctggtaca
    15961 aaaaggttct cggtttattg taattaagat cgccggattg ataaacttaa aaaaagaacg
    16021 cccgacagaa aaactgtcgg gcgttttgaa ttagagaatg cggaaaaatt gaaaattttt
    16081 ctcgcacctc catagtccag taaaagccaa gctatccttt aaccaagtat agcttggctt
    16141 ttaccttctt aaggtcggac acggaaaatt ctcgaaattg tagatttcgg aattttccgt
    16201 gtcctcttaa ttagtctttg tatccaatct cactgttggt tttgtttaaa gtaagtgcat
    16261 ttgaaagggc aattccgcct tttacctgcg gctggttaac aacctgtccg ttgacataaa
    16321 gaacagttga acctccgccg tcaaggttca ttgcgtttat gcaaccgatt gatttcataa
    16381 aatttgcaag ctccattaaa gtcattccca cagagctgct ttctcttccg tcaactgtaa
    16441 cgagaatcaa gttattgtct gcggtatagc ctattgcgct tcttgggttt ctgccaccga
    16501 ttgaagcaag cttttgttct gtcatatcca caaaaacttc accgtgctta acaagataag
    16561 gtccgccgct tattatgtga ttaactccct gccactcggg aagtgttgcg acatcaaagc
    16621 gaatgttttt tgccccaagt aaatctttta accttgcttc cggagccact attacaaaac
    16681 cgtcttcggg tattgcaagt gcagaagttg aaacttctat aactttgtta tctttaacag
    16741 cgatttgttt tccgtattta ggcgaaggcg gcgcaaattt accccattct cttgtataga
    16801 caataacgta agtcgaaagc attcttggct ggtttatatt atcgactttt acactgtgta
    16861 agcctgattt taaagtcgcg tcaagctgaa ctcgagccat atcgtattta ttgtcaaaaa
    16921 tacccattgc tactctgtta tagataggcc ctgtgtacat tttcttgtta atcattaaag
    16981 tcccgagggg aacgcctgtt gcaggcttaa agaatgtacc gttgattgcg actattgaat
    17041 tgtttttctt tgctatgctg cttattgatg ctttatttgc taatgtttct gatgctatgg
    17101 ctggggttaa ttgaaggtcg gggttaatct ctgaatttac ttctattacg tttatcctta
    17161 caggttgtcc ttggtaatat ttggttatcc ttatgtgttt tactccctta tctacttctg
    17221 ttatgattcc tgtcttatac tttcgcttta tttcctcatc aaatgccttt gtcttttgct
    17281 ctatgctaag ctgttttgct acatttgcct ctagcgttac cgtgttaaaa atcggagata
    17341 tcgcggtatt gtttgcaagc ttaatctgct cttgtgcgat aagcggcggc ccctcaaaaa
    17401 catgctgagc gaccaaaacc cctaaaacaa cacaaaagtt aaccgtgtga acgattaatc
    17461 ttgttaattt ttttggagtt aaaaccaagg tatccatttt tctcaccaac attaagtact
    17521 cggatacctt ttttatttga gagtggaggg taaatgaggt tctagacctt ttgtgctttt
    17581 ttgatttggg gcttcaagcc tcattacttt gtgggggaat aacactctga aaaaaccttt
    17641 ttcatctttt tcaggctttc tactactatt gtaacataaa ttcacaatta tctcaagtgg
    17701 ctgcaagcct tttacaataa gaattttaca aaactaaata gtgtaaaagc tacattatta
    17761 gtgagtaaat agtaaatagt aaatggtaaa tagaagtgaa taagaggata agaggagaag
    17821 acgcgtagaa gcgtagcaga ataatccacg catctacaaa tcttatcttc ttataaactt
    17881 ttcaactatt cgactattcg acttttcaac tattcatcca acaaaatcgt ctgttcacgc
    17941 tttggaccga cacttactat tgatattgga atttcaaaaa gttcttccag tctttttaag
    18001 taattttggg cgttgagcgg caattcttcc cattttctgc agtttgatat gtgttttttc
    18061 cagcctttgt gcgtttcgta aatcggctca agatatttgt gaacaaaaac gtttgtagga
    18121 taatttttat aaattctacc gtctcttttg tctttataag ctgtacaaat tttaatttcg
    18181 tcaaaatcgt caaacacatc aagttttgta atcgccattg aagtcaaacc gcttaccaga
    18241 acggcatatt tggcgattaa aacgtcaaac cagccgcaac ggcgcggacg tcccgttgtt
    18301 gtgccgaatt cccgcccgat atctcttatt ttgccgccca cttcatcttc aagttcagtt
    18361 ataaaaggac cttcgccgac tcttgtaagg taagcctttg atacccctat gatttctttt
    18421 attgaagtcg gccctaagcc gcttcctgta caagcaccgc ctgctatcgg gtttgagctt
    18481 gtgacaaaag ggtaagtacc gtaatctata tcaagcatta cgccctgcgc accttcaaaa
    18541 agaattgtct tatttactct ttttacttca ttcaatgtgt tttgccagtc aaaatcaacg
    18601 taagattcaa atattttggc atattttgcg caaagctgcg ttatttcttg ttttgtatag
    18661 gttttaagcc cgaaggtctt aagcattttg tttttaatcg gaagaatcac gtcaagtttt
    18721 tcactcagag cctcgaatga gtataaatct tctattttaa gcccccaacg tgccactttg
    18781 tctgtgtaag tcggacctat tccttttttt gttgtcccaa ttttgccttt tttggctttg
    18841 tcttcggaat atccgtccat ttcaatgtga tagggcatag taattgaagc aagagggctt
    18901 accttcaagc ctttagggct tatgttttgc ttgattaaat ctttgacttc ctgctcaaaa
    18961 gtttcagggt gaattactgt tcctgcaccg ataaaacagg ttttgccttc gtataaaatt
    19021 cctgagggta taaggtgaaa tttgtaggtt ttatcctcca caacgacggt gtgccctgca
    19081 ttgcagccgc cttggtatct tatgattaaa tcggcttttg tcgccaataa atctgttatt
    19141 ttagcttttc cttcgtcgcc ccactgggct ccgaccacta ccgtgttttt catttacccc
    19201 tcatttaccc ctcatttacc ccttcagcat tttaatcaat tcgggaacaa cttcataaac
    19261 atcgcccaca attcctatat cggcaacgcc gaaaataggg gctttagggt ctttgtttat
    19321 tgcgatgatt ttttcagaac cgctcattcc gactgtatgc tgaattgcac ctgaaatggc
    19381 acatgctata taaagcttcg gcataacggt tttgcccgtc tgcccgactt gaatggcatg
    19441 atctgcccag ccagcatcca cgcagggacg gcttgcgccg actgctgctc ccaaaacttg
    19501 ggcgagttct tcaagaagct taaacccgtc aggcgatttg agtccttttc cgcctgccac
    19561 aataatttca gcctcatcaa ttctagggcc tgatgctgcc tgaaagtttt ttataaattc
    19621 tacaatctcg acttttttgt ctattccatc gatatttgaa gggaaatcta taaattgagt
    19681 atccttaact ctgggttctg atgctttttt caagactttc ggtcttacag atgccatttg
    19741 aggaaggttt ttgcacaaaa ttgttgccat taaatttccg ccaaatgtcg gtctggtggc
    19801 tgcaagctga cctttttcat taatatccag tcctgtacaa tctgcagtca atcccgtatt
    19861 aagagttgac gagattcttg gagcaatatc tcttccctga gtggttgcac cgattaaaaa
    19921 gattgacggt ttaacctgcc tgaccaaatc tattgcaatt tttgaataaa gctcggtcga
    19981 ataatctctc aatctgtcgt cttgaattat ataaacctta tcaaagccgt tatttctaag
    20041 ttcgttttta atgtattcaa tatttccggt ttttgtgata agtaccgctg aaatctcttc
    20101 tccgcccagt ttttgtgcca aatcgtaagc tgtatttgca agttccagca caaccggagc
    20161 tagagttccc tcccttgtaa tttctgcgta aattaatatt cctttgtttt ctttttcgtt
    20221 ttccattttt tcctcattct acatttttgt ggcaagtgtc attattttac tatttcaaac
    20281 tcttttaatt tttctttaat aatttccgct cctttttgag gggtttcagg cgtaataaca
    20341 tctccgccct gcttttgttc cggcctgaaa gctctgctta cataagtagg agagccttta
    20401 atgccgacct gctctggctg aagcttaatg tcttctgcgt tatacatatt aatttgggca
    20461 ttctgcgctt taacaaagcc gtttatcaaa ggtcttctgg gctcataatc gcatttgagc
    20521 atacacaaaa ccgcaggaag tgtggttctt attatttcaa ttccgtcttc gacttctctt
    20581 tttacggtta aaaacccgtc tttatagtca acaatttctt taacgtaagt tacctgaggg
    20641 aaatttaact gctgagctat gctcggccct gtctgagccg tatcgccgtc tgttgcgaat
    20701 tgcccacaga taatcaaatc aacatctttg actttttctt ttatggcagt ggcaagagtt
    20761 ctgcttgttg caacggtatc cgcaccggca aacttcttat ccgaaactaa aaatccttcg
    20821 tcaactccca ttgaaattgc tcttttaaga atatcctgcg cctgcggagg ccccattgaa
    20881 agtacggtga tgtggacatc tttgaaattt tcttttaacc tcaccgctgt ttcgattgcg
    20941 tacatgtcaa aagggttaat tacgctttct acgccttccc tttgaattgt gttgttttcc
    21001 gtccatttta tatcggctgt gtcaggtacc tgtttaatac ataatgctac tttcataaat
    21061 acaactttcc ttctctatct caattctttt tgggctgctt gctttgaatg tccaaagcag
    21121 cattgtgcgc caaaacatac atagcacaca ttctgtaatt tttcaaatac caggcgcatc
    21181 tttcctgtgt gcaaattatt tctattgtgt ttcccgcgct cataagcgga cagtaaggta
    21241 tgtttttttc actcataatc tactccttca ccttttgctt gaacaacttg caattttcat
    21301 catgtttttg ttcctgttct ttataaattt tcattctgtt aataacttca tcaaagtcta
    21361 tcttatgtgc gtcaaagtca ggaccgtcaa cacaggcaaa tttaacttca ccgccgactg
    21421 tcaccctgca agccccgcac atgcctgttc cgtcaaccat aatcggattc atgctggctt
    21481 cggtgtaaat tcctttgtct ctggtcatat tcgcaaccgc tctcatcatc ggcataggtc
    21541 ctacggctat tgcataatct actttttcag aattcattat ccgctccagc acctgcgtta
    21601 caaatccttt ttcgcctaaa gtcccgtcgt ctgttgttat aaaaagctca ctgcaggctt
    21661 tttccatttt ttcctgccag aaaatcaatt ctttatttct tgcgcccata atcatataaa
    21721 ctttgttccc tgcttcttta aaagcttttg caatcaaata gca
//
LOCUS       DAOQAU010000028        21277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2541_length_21277_cov_6.120535, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000028 DAOQAU010000000
VERSION     DAOQAU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_21277_cov_6.120535"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="PKI94_05350"
     CDS             <1..1565
                     /locus_tag="PKI94_05350"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNW26202.1"
                     /translation="PLLSEQWFVRMEPLAKDAIAAVKDGRIKFVPERWEKNYLGWMEN
                     IRDWCISRQLWWGHQIPAYYHNVTGEMVVAKENPDPKNYTQDSDVLDTWFSSGLWPFS
                     TMGFPNTESEDFKKFYPTSVLVTGFDIIFFWVARMITMGLEFTGKAPFADVYIHGLIR
                     DEHGQKMSKSKGNTIDPVVIIEKYGCDALRFTLTSLCTYGGQDIKVSDERFEYGRNFA
                     NKIWNASRFVLMNLEGIDDKEIDFDNLTIADKWILDKLNSVSKEVNENIKNYRIGEVA
                     HILYDFFWNSYCDWYVEIAKIQLSDKKLKNNTQRILRYVLDMSLRLLHPIMPHITEKV
                     YSLIPNKGFKAIMLADFPAYNKFLKFPQESTEMELVFETIKAIRNVRQSFNIAPSVKV
                     NIEIRPEKNEKPIFETVLSYIKRLARVEEIKFGEEKQPLPKKSATAVVRASKIVIPLE
                     ELIDIDAEISRQQKKLDKLLAEKKSLEGRLNNKNFVDNAPKEVVEETKTKVAEISLQQ
                     EIIEKLITSLKD"
     gene            complement(1562..1894)
                     /locus_tag="PKI94_05355"
     CDS             complement(1562..1894)
                     /locus_tag="PKI94_05355"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNW26203.1"
                     /translation="MINTNFSIYSNDNRYKAPSFKGTAEKLAQKLLKATNPELRKFPV
                     TFDEAVALYEKMGFEITKKGGSHAVVTLSGTDLSFPLLIPHHGEKKINPIDVKKLKDM
                     VVVYYKND"
     gene            complement(2338..2610)
                     /locus_tag="PKI94_05360"
     CDS             complement(2338..2610)
                     /locus_tag="PKI94_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26204.1"
                     /translation="MGFNINPPSNKPVIREAANMQNDGGAGNLGYFEQGEGEKKKQES
                     IFAKEESDSFKKEGDINLPDEPFSFAKLVADIIFAVKEWFKKTFKL"
     gene            2842..3876
                     /locus_tag="PKI94_05365"
     CDS             2842..3876
                     /locus_tag="PKI94_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNW26205.1"
                     /translation="MRVNFQGVYSTFTPVAGGISRDKFYTIPQNRLSSDVFVPSSSTI
                     SFGRLTTKFPFALLKDIPCPYCGIKMVTEADFEKYLTKETLSGTGRMAVKAITRFEDS
                     MQEPEKRIFQKLQELSKKNPLSNLQDLLMSVMPESLKRLQTTQLNILNKLAQLSPSLP
                     KEYSNELNMIVKRHREIINSPDNNIRFKRKNLLNSIYNLQLSLPDKKMARELENIAYS
                     MPNSMNHYDSFVVKYSERTPFEIGQRLVRPSMGTFEHVHPHSHGGTADLSNGLCVCGK
                     CNHTRGNLPFSDLVDSHPEIKTNIKQHLQLIGKIIKKRDMASLKDYVKNIAETIKNES
                     EGKIDLTKII"
     gene            complement(3896..5071)
                     /locus_tag="PKI94_05370"
     CDS             complement(3896..5071)
                     /locus_tag="PKI94_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNW26206.1"
                     /translation="MKFKYFLILFFSIFLISGCTKQPQKTVIKFSSWGSQSEIAIIKP
                     ILAAFEKENPDIKVEFMHIPQNYFQKLHLLFASNLAPDVIFINNLYLPIYADANLLDT
                     LNGKIDENLFYKKALDVLSYNNKLYAIPRDVSNLFFYINKNIFDRYNVPYPSKYWSFN
                     DFLILAKKTTKDTNNDGKTDIWGVSFEEDLFFYLPYLMSEGGGVLSDNLKHLIIDTPQ
                     SQKGLHFYADLRNKYNVAPRKSESASATMAQLFLQGKLAMHLSGRWLVPKYRQEAKFN
                     WGVINFPKGDKGSIVPMDASGWAISKSSKHKQEALRLIEYLSSKKSIEKMTQSGLIVP
                     ARIDVAHGEFLQDKNSPYNKTFVEVIKTSKKTPVSIKYSEIQDDLKEKTEYLFNQIK"
     gene            complement(5071..>5931)
                     /locus_tag="PKI94_05375"
     CDS             complement(5071..>5931)
                     /locus_tag="PKI94_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26207.1"
                     /translation="ETETETETDVKPEQETLIEVEETSVENPVEQEEIIEEKQEEVHE
                     TTDLEDFLEGKEEVQEIEEIIEPQVQEAAFEEVDLETLSFEETLPSIQQQDEESTTFE
                     PLASLEELTEEAEKIEQAEQIQEVEDLSALEKATIEEIPSTISLDEFEVEEEPKTVKK
                     EEELIEDLFEAEEEPLVEEETVDEEEIERKENEILLNSLGLKSRSEFAVDCEKGFHLV
                     DFEGTTALIGYINDEIFVLKRFDETIKSPLQVRLSERKADKEDYMVKAGSFKAIIEVS
                     PYNMELVIEL"
     assembly_gap    5932..6031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6032..7491)
                     /locus_tag="PKI94_05380"
     CDS             complement(<6032..7491)
                     /locus_tag="PKI94_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26208.1"
                     /translation="MKKDFNKSKYGLSVGVALLLSQFAAFGGDLFDNNLLKVDLNKTS
                     PAGVKVTLYTKNPYKEALTVNKKSDFQYVILLPETVSSITSKPSLSATSDVIQNIEVK
                     TQQLSQEASKGYTKIIISTTKPIEIVAQNQVLSTSDYKVSESDYKELIAQAGKKSVAQ
                     KSAVSAAKKAEPVKKATTPAKKSTVQPAKTAKPVIKKQAIPVAKPKPKVAAPAVQKPV
                     NKPPVQKSVEQKPVVQPAKNIPATTPAPVTTTAPAPQPQTAPATTPTAEQALPAAQPI
                     PAMPQKVEMPLPETPFQKVMNIIKANLTMFAGGALLIPLILLLIFMKKGKKPVAEIKE
                     EKTQVQETSEGMPNIQQLQEEVVEEEVIEEGLTEDELPETEEIIFPQEEIITDKKEEI
                     DTLFNQDTANDILTKEHQKQAEDVEENTEREPVIETVPETSSPAEESFEEILPLEDII
                     PLENIEPIDEIEQMEGAVAPEPESELESETETETET"
     gene            complement(7761..10007)
                     /locus_tag="PKI94_05385"
     CDS             complement(7761..10007)
                     /locus_tag="PKI94_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26209.1"
                     /translation="MEIKLKTEHSFSKGRFRAREEISIPKLSQKIFNNKEERPFNSNS
                     KNLSFKGLFIGPFRKVSIAEVDSLLQLTDKHVGKNIRELYEHLVNSNVAKKLIKLEGE
                     NLLIKEKSIPELIADGTIYPIKHLPKDILNGVVGVLRKFGPTKQWAQKTYSSEFFKKI
                     RQNSKVESKVNALKGIFESFEKLKGKDSDEISLESVKKISKIFDEKTGNYDTKHERAL
                     NRIVSGIIPAVFLANDAYNLSRMCNDNKKDANKEKKIRFRQELSRIVLNAYIMLITYG
                     ALQKQMNKSKSAVMLMTFATTLFTESISRIVNGKHVVRLSKEKAKEINKNGKDSSTAE
                     QAKSQEQISQNHDYKNVFFKSQNNREVFKAFMGRLEDKVTLKSTEPKAIIKTQQDKQK
                     EPLLSPNTLFKACLAVIAAGFAVKGIRSIKVKPEITIGKFIDKQIFAPFKKVYEKLTM
                     NFDNKIKAEDFDEIVSKLKQVGHDTLANKYQEIAAKHKTLVGDIEYIQLFKHKKKTKP
                     LVDFVIAPFKFAYGAVTLPYKLLNKVYAAVSGKKPPLKEITELEAFSKSIEFIGKAAQ
                     DKNYTPIKFKSYMDDNIAKAFNECNVSGISNSDLSNLAKTSATAATLWFLMADNYNMV
                     MLKSNGEDKEGAKLKFKERFVQEMSRLFYQTLLIDLFNSTFRSQYNSSLLGMTWITIS
                     NTTIGEILTRKSVGMPVMSHSRDELIEIDKKKENATGFLKGYYNFMTRLTGKKSLSEQ
                     YKTKQEKK"
     gene            10294..10731
                     /gene="rpiB"
                     /locus_tag="PKI94_05390"
     CDS             10294..10731
                     /gene="rpiB"
                     /locus_tag="PKI94_05390"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNW26210.1"
                     /translation="MKKLKIAIGADHGGFELKEKIALMLKSQGYEIEDFGTFNSDSCD
                     YPPIAKKVAKEVASGNFARGILICGTGIGMSITANKIKGIRASLCHDKFSAQMSRAHN
                     DANVLCMGQRVIKEDLALEILNIWLNTDFEGGRHKKRIDIIED"
     gene            10732..11127
                     /gene="rsfS"
                     /locus_tag="PKI94_05395"
     CDS             10732..11127
                     /gene="rsfS"
                     /locus_tag="PKI94_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNW26211.1"
                     /translation="MDKIDSQMLASIIARILDDKLAKDISILNISNVSVMADYFVICS
                     ADTSTQVRALSSYVREKIKMLFERIPSGEENDLKNRWNLLDYGDVVVHVLHREERETY
                     AIEKFWSHALKMPEEQWKELSADFSEYTR"
     gene            11132..12004
                     /locus_tag="PKI94_05400"
     CDS             11132..12004
                     /locus_tag="PKI94_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HNW26212.1"
                     /translation="MKRNIVKFNGGLGNQMFQWAFARALEKITGMPSFMDMTYFSRTY
                     ARPYELDIFAAEIKKIKGFFSELMLETLWKLRREFDKKSFLGTYLYEEPHFGFDKNVF
                     KIGKSAFIHGFFQSEKYFKKIEKQIRKDFTFKSEPDFQNQDFLEKINASNSISLHIRR
                     GDYVQKKKYQKLYASCSLEYYKRGVEYIAQKFENPTLFIFSDDIDWAKENLKLPYKNY
                     FVAHNLEAKSFEDMRLMSACKHNIIANSSFSWWGAWLNQNPEKIVIAPQKWFNDESIN
                     QSDIIPENWIKLGN"
     gene            complement(12009..12350)
                     /locus_tag="PKI94_05405"
     CDS             complement(12009..12350)
                     /locus_tag="PKI94_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26213.1"
                     /translation="MKINNVSFTGGILIRGTQKQHEEITKMLKDVFKKDDSLLSLPQS
                     IGKKSVVLYAIQDTALKVKRYLPFDYQKYDAVMINPKNFGVKKLVRSAQTVLEAMKNH
                     TFDFFNLAIYK"
     gene            12499..13278
                     /locus_tag="PKI94_05410"
     CDS             12499..13278
                     /locus_tag="PKI94_05410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW26214.1"
                     /translation="MAKISVVINTLNAQRLLKECLESVKDAYEIVICDMYSEDKTIEI
                     AKEYNCKIVYHERTGIVEPARNWAMEQATGDWILILDADEIVRPELWEYLKKYADNPK
                     PSHFACYLSRETAMDGKILKSWFQKGVKRFWKKGICTYTDAIHQMPITHEGKDFWIVG
                     KNLTMIHYHMPSISSFNEKIDRYTDFELKRFEENDKKFSPFKLYTRSCFEFFKFYILK
                     GGIFDGIHGFIFAKMQAYYKFIQWAKLYEKEFKEKNKDLLY"
     gene            13281..14201
                     /locus_tag="PKI94_05415"
     CDS             13281..14201
                     /locus_tag="PKI94_05415"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HNW26215.1"
                     /translation="MEKIQIALTYDDNYAQHSVVLIASILENKTGNEEIIFHILDGGL
                     SDNSKKILQDIQGCEIKIHQVDKKQFEGYVKSDYYPVSMLWTMILPDIIKVEKLIYLD
                     SDMVVNSSLKELWETDLSDNYVAAVEDANGKKYAKRYGLKDSKFFNTGTMLINCKKWL
                     EDNIPQKAVEIAMKNTGSRWGYDQTVLNQLFEGKVKFLDLKWNLQYFPINVWATYDDK
                     QEYKNAIKNPLIIHYVGDYKPWKKGLGCFNPKQQDYFKYHKITPYAFTDYNKWFRQDK
                     LLSYKGLAAFIKRYPLFFLKKQFWKNMFSI"
     gene            14255..15310
                     /locus_tag="PKI94_05420"
     CDS             14255..15310
                     /locus_tag="PKI94_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26216.1"
                     /translation="MDRVELEEKIKEAIVELAKEPEKVEHYHKLANLYAMEKNYDKII
                     SVYEGLLNVNPNDTDALMNLGSLAFYRKNYEKAIEYYLKVLDIKPNDYILYFNIGNVH
                     AELKNFSKALYFYNRALDYNPSNPEIYNVMGLLFHDFEDFVEAKIYYEKALSLDPNSI
                     NAHIDLGNIYFKESEYEKAMEHYKKVLELSPNDKTVVICVANILVATKNKKEAFKYFD
                     KALKMDENNYKTYVCMAIAYSEFMEYDKAEEIYKLLIEKYPKNINPHILLGNLYSNIK
                     KHDKALNQYQKALEIDPSKASVHMLIANTYYIKQNIEEALTSYRKAIRFEPDNDEYKL
                     VYIQILDEYISKKEENK"
     gene            15307..15726
                     /locus_tag="PKI94_05425"
     CDS             15307..15726
                     /locus_tag="PKI94_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26217.1"
                     /translation="MKNYGAQQPLMIEKIIAGLSYITFGFVGFIWLLIGIFTKNSLRP
                     YLKYHIFQSIFISIAYFLVCQFLGLILNILSIIPFINQIVMQITLYLNMPLFFGFSII
                     QVAIYAVIFYLVATSFQGKYSYIPWISEIIKANVRNS"
     gene            complement(15723..17960)
                     /locus_tag="PKI94_05430"
     CDS             complement(15723..17960)
                     /locus_tag="PKI94_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW26218.1"
                     /translation="MQILKISFTGERRNNRIPYQRTAGNTPPIKLPNQKHSDTFVKGD
                     SYNFSTEPQFYETLEENYFKLPRGCYPDEYQKKAAAKLYKGEDVLVTAPTGTGKTAIA
                     YYVITKNLSEGKKTFYTTPLKALSNQKYRELQTLYGKDNVGLLTGDIKINADAPIIVM
                     TTEIYRNMLFSKKFQDNPDNLLKLKTVVFDELHYLGDVDRGGIWEQSIILTDKNVQLL
                     SLSATIGNNNQINNWMSDVKGKKSNLIDVPPEKRHVPLVFETHNLTGENVEGRKLKKH
                     QHQKSKDANDVKKAYIKFISELKGKDRLPAILFIFSRKFSKKLLDEFDKSTLQLTSRK
                     EKEEIQKIIDQYTKNGKYLGETLNTNALLKGYALHNAGLLPTQKELVEELFQKKLVKL
                     VIATETLSAGINMPAKTVIISDTRKPTSAQSADGDDGKREITANEFHQMAGRAGRRGI
                     DKIGYVYTTPKDKEEKEIFNILIDESPNSLESAFNPDFSFVAGYCKHYQDDELIKTIL
                     KKSLHSYDTNPKASEKKYLSMFKLFENKREMLKHLDYITSDNKLTTKGQLLAKINGYQ
                     QLPIIDLIYDKKLATLNPIELAAVVGSLANLSQKDDYNKETEFFGHKNDKISWFVDDF
                     DSYLDKFNTLNKNSEVAQDKDAVKHLYEWAELNSKFSGSRRNWKGLYCGVLHKTIRDE
                     GSLFKEITQTVDLLKQMRNISKKGYQLAELENNKNDMEYYKQLSGTIGESLKLLLREP
                     ITEII"
     gene            18085..18888
                     /locus_tag="PKI94_05435"
     CDS             18085..18888
                     /locus_tag="PKI94_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26219.1"
                     /translation="MKKIFLLILVLGFFGFQVCLADELDEASGINDENAISNESETTN
                     VEEINNIFTNDPQNTMELHGYLEYDEIPDNTVYLDQASKKSPDFKPPIKITSKSLLTP
                     SNKKPTIPQYKTLDLVSKFSTQEYDINEINSNYSEKAGRLTFGTMYNSDLDNAQINYS
                     TGFFTRYDWKKFAIASGYSRSTNSNYDSYYDKVYFAPEFKITKRLSILDIVQSDVQQI
                     KKKNEIVLRYTPNLKRYADEVQFEIGAGQYFREDEYVNSAIRFSTRFKL"
     gene            18962..19453
                     /locus_tag="PKI94_05440"
     CDS             18962..19453
                     /locus_tag="PKI94_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNW26220.1"
                     /translation="MTKESDKSRKKRNSTPARNVHRANNPENETLIDKTKIKNKQRKN
                     RIRFYYSFLTVVLLICIAQIGFSAILNISKTISYQTKIATMKKTKNQAEVRNKKLKEE
                     IDNFSSASSLEAIARNNLKMAGQDEVLVIINTAQEANSEKSDKKNNKTKKPKSNKNKK
                     DNR"
     gene            19456..21159
                     /locus_tag="PKI94_05445"
     CDS             19456..21159
                     /locus_tag="PKI94_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26221.1"
                     /translation="MNNEVVYYVKNSFELKSQGHFKPAIEMLYKALAIEPENLEILVQ
                     LAHLYKLLGNLEKCFYYAEKVLEIDKNHIECLLLIKDIYISQEKIKEALDIADKIFEA
                     KPFNENLAQKIKLLNILNDSQQIEKIEKNLKDADDKVFFELADFYFNNKNLEKALEMA
                     ISGYAKNQKNKDLLMLLGKIYFEKNDFENAQKYFEEYAQKEKTPEVLNYLGLLKLNFL
                     QYQKATEYFAKAFELDEKNSEYSYNLASANFLRGWFDEALKYFTLAISLNPERLDYHY
                     SLAYLYYQKKAYEKALIELNYILSKENFAMATTLKSMIKAKQGNIIEAKSDLENILKE
                     NPEDDFALSALGKIYSELKNYDAAKNVLEQATLIKPESLDYISDLTEIEIESRNFDRA
                     LELANEIIKKNGKYVHAYVLLAKIYFAKNEFDKVFNAAQDIIELDSNNPEGYYYNSLS
                     LFEQGDVNFAIESLKKAISLDLNNSALYIKMSEFYQELGDFKQAYAWAKEACDIDEAS
                     YQTKWLCAKLAAQLKEEKAAVNYYSHSYRLASFDKDLVQEYSQYLKSIGKEKQAKALL
                     K"
BASE COUNT         7062 a         3510 c         3485 g         7120 t
ORIGIN      
        1 agccgctttt gtccgagcag tggtttgtaa gaatggaacc tcttgccaaa gacgctattg
       61 cagcagtgaa agacggaaga ataaaatttg tacccgaacg ctgggaaaag aattatctgg
      121 gttggatgga aaacatccgt gactggtgta tttcgcgcca actttggtgg ggacatcaaa
      181 ttcccgctta ctatcacaac gtaacgggag aaatggtcgt tgcaaaagaa aatcctgatc
      241 ccaaaaatta cactcaggac tcagatgtgc ttgacacctg gttttcatca gggctctggc
      301 cgttttctac aatgggattt cccaataccg aaagcgaaga ttttaaaaag ttttacccta
      361 caagcgtttt ggttacaggc tttgacataa ttttcttctg ggtagcaaga atgattacca
      421 tggggctgga attcacgggc aaagctcctt ttgcagatgt ttatatacac gggcttatca
      481 gagacgagca cgggcagaaa atgtcaaaat caaaaggaaa taccattgac cctgtcgtga
      541 taatagaaaa atacggctgc gacgcgctca gattcacgct gacctcgctt tgtacatacg
      601 gcggacagga tattaaggtc agcgatgaaa gatttgaata cggcagaaat tttgcaaaca
      661 aaatttggaa cgcttcaaga tttgtattga tgaaccttga aggtatcgat gacaaagaaa
      721 tcgactttga taatttgacc attgcggaca agtggatttt agataaactt aattcggttt
      781 caaaagaggt taacgagaat atcaaaaatt acagaatcgg cgaagttgcc catattttat
      841 atgacttttt ctggaattca tattgtgatt ggtatgtcga aatcgcaaag atacaacttt
      901 cggataaaaa attaaaaaat aacactcaaa gaattttaag gtatgtgctt gatatgtctt
      961 taaggttgct tcatcctata atgccgcaca taactgaaaa agtttattca cttataccga
     1021 ataaaggatt taaggcaatt atgcttgcag atttccctgc atataataaa tttttgaaat
     1081 ttccgcaaga gtcaacagaa atggagcttg tctttgaaac aatcaaggca atcaggaacg
     1141 taagacaaag ttttaatatt gcaccttccg ttaaagttaa tatcgaaatt cgccccgaaa
     1201 aaaatgaaaa accgattttt gaaactgttt tatcttacat aaaaagactt gcaagagttg
     1261 aggaaattaa attcggggaa gaaaaacaac ctttgccaaa gaaatctgct acagcggttg
     1321 tcagagcttc gaaaatagtt attccgctcg aagagttaat tgatattgat gcggagattt
     1381 cacgtcaaca aaaaaaattg gataaattgc ttgccgaaaa aaaatcactt gaaggacgtt
     1441 taaataacaa aaattttgtt gacaacgcac caaaagaagt tgttgaagaa acaaaaacaa
     1501 aagttgctga aatatcactt cagcaggaaa ttattgaaaa attaattaca tccttaaaag
     1561 actaatcatt tttataataa accacaacca tgtcttttaa tttttttaca tctataggat
     1621 taattttttt ctctccgtga tgaggtatta agagtggaaa agataagtca gtaccggaca
     1681 atgtcacaac cgcatgagag ccgccttttt tggtgatttc aaatcccatt ttttcatata
     1741 atgcgacagc ttcatcaaaa gttacaggaa attttctaag ttcggggttt gtagccttaa
     1801 gaagcttttg cgcgagtttt tccgcagtac ctttaaaaga aggtgcttta tatcggttgt
     1861 cgtttgaata aattgaaaaa tttgtattta tcatttatct gccttttctt ttttaaaaag
     1921 ggtgaaaagt taaaattgac agacaaggca tagaaaaata caaaaattta caattattaa
     1981 tgcggaattc gttcgtctgc tcgcttgaac tgcgctcgca ataaacggca acgctcacaa
     2041 caaaatcgaa aagtgtgatt ttcgattatt gttgttcgac tctcacttcg ttcgtgcctc
     2101 tgctcgcttg tgtcactatt gtaaaatcat gctaaagaat ggactattta tgcattccgt
     2161 gacgccattt tctacggctc gttcctcgcc ttgaaaagaa ggcaaaaaaa agaccttgaa
     2221 attgttcaag gcctgtccgt ttaaataaga agagagagct ttatactata ataaagtccc
     2281 ctcaatttta aaaatttcca tttttgttca aattttaaac aaaattgaaa tattttgtta
     2341 caatttaaat gtttttttga accactcttt taccgcaaag ataatatcgg caacaagttt
     2401 agcaaaagaa aatggctcat ccggaagatt aatatcacct tcttttttga aagaatcact
     2461 ttcttcttta gcaaagatgc tttcctgttt ctttttttct ccttcacctt gctcgaaata
     2521 ccctaaatta ccggcaccgc cgtcattttg catgtttgca gcttccctta ttaccggctt
     2581 gttacttgga ggattaatgt taaagcccat aactatacct cattttaaaa aaccagttta
     2641 attataatgt atgtattgta aatttgtaat cctttttttg tattatatta aaaaatgtta
     2701 atttaacaaa aatcactgaa tttacaatgt ttttaaacga atagtgcata gatactgctt
     2761 attagtcata tgtactattg gtttgtaaat ttttatgaaa aactaacaaa aaaagtcttg
     2821 tctgcggttt ataataacgt tatgcgtgtt aattttcaag gagtttacag caccttcaca
     2881 cccgttgcgg gtggaatttc gcgtgataag ttttatacaa tcccccaaaa taggctttct
     2941 tcggatgttt ttgtcccgag tagcagtacg atttcttttg gcaggctcac gacaaaattc
     3001 cccttcgctt tgttaaaaga tattccatgc ccttattgcg gcattaaaat ggttacagaa
     3061 gctgattttg aaaaatattt aacaaaagaa actctctccg gaacaggcag aatggcggtt
     3121 aaagctatca ccagatttga agatagcatg caggagcccg aaaaaagaat atttcaaaag
     3181 cttcaagaat taagcaaaaa aaatcctcta agcaatttgc aggacctttt aatgagcgtt
     3241 atgcctgaaa gcctaaaaag actacaaact acacagctta atattttaaa taaactggcg
     3301 caattaagcc caagcttgcc taaagaatat agtaatgaat taaatatgat tgttaaaaga
     3361 cacagagaaa taattaactc gccagataat aatataagat ttaaaagaaa aaatttatta
     3421 aacagcatct acaatttgca attaagcctg cctgataaaa aaatggcaag ggagttggaa
     3481 aatatagctt acagcatgcc caattctatg aatcactatg attcttttgt tgttaaatac
     3541 tccgaaagaa ccccatttga aataggacaa aggctggttc gcccgtctat gggtactttt
     3601 gaacatgtcc acccgcactc tcacggcgga acagctgatt taagcaatgg actctgtgtt
     3661 tgcggaaaat gcaaccacac aagaggaaat ctgccttttt cagaccttgt tgattctcat
     3721 cctgaaatta aaacaaatat caaacagcac ttacaactca taggcaaaat cataaaaaaa
     3781 cgagatatgg caagccttaa agactacgta aaaaacattg ctgaaactat aaaaaatgag
     3841 tctgaaggta aaatagattt aactaaaata atttaaaatt aaaggaatat ttttattatt
     3901 ttatttgatt aaaaagatat tctgtttttt cttttaaatc atcttgaatt tcactatatt
     3961 ttatagaaac aggagttttt ttggaagttt ttataacttc tacaaaagtt ttattatagg
     4021 gcgaattttt gtcttgcaaa aattcgccat gcgctacatc aattcttgca ggaacaatta
     4081 atccgctttg agtcattttt tcaatacttt tttttgatga taaatattct attaatctga
     4141 gagcttcttg cttatgtttt gatgatttgg aaattgccca gcccgaagca tccatcggaa
     4201 caatgctccc tttgtcgcct tttggaaaat ttatcacgcc ccaattaaat tttgcttctt
     4261 gtctgtattt aggcacaagc cagcggcccg aaaggtgcat tgcaagtttg ccttgcaaaa
     4321 aaagctgtgc cattgtagca cttgcgcttt cagattttct aggagcgacg ttgtatttat
     4381 ttcttaaatc agcataaaag tgaagccctt tttggctttg gggagtgtca attatcaaat
     4441 gtttcaaatt atcggataaa acccctccgc cttcgctcat caaataagga agataaaaaa
     4501 acaaatcttc ttcaaagctt acgccccaaa tgtctgtttt gccgtcattg tttgtgtctt
     4561 ttgtcgtttt tttcgctagg ataagaaaat cattaaaaga ccagtacttg cttggatacg
     4621 gcacattata tctgtcaaaa atatttttat ttatatagaa aaacagattg gatacatctc
     4681 gaggtattgc atataattta ttattatatg aaagaacatc aagagctttt ttgtaaaata
     4741 gattttcgtc aatttttccg ttaagggtat caagtaaatt tgcatcggca taaatcggca
     4801 gatacaaatt atttataaaa attacatcgg gtgctaaatt tgaggcaaaa agcaggtgaa
     4861 gtttctggaa ataattttgc ggaatgtgca tgaattcaac ttttatatcg gggttttctt
     4921 tttcaaaggc tgctaaaatc ggtttaataa tggcgatttc agactgtgaa ccccaagagc
     4981 tgaattttat gacagtcttt tgaggctgtt ttgtgcagcc gcttattaaa aatatcgaaa
     5041 aaaataaaat taaaaaatat ttaaacttca ttacagttca attaccaatt ccatgttata
     5101 agggcttact tctattatag ccttgaagct tcctgctttt accatgtaat cttctttgtc
     5161 tgcttttctt tcgcttaatc tgacctgcag cggcgattta attgtttcgt caaatctttt
     5221 cagtacaaat atttcatcat taatatagcc gataagtgct gttgttcctt caaaatcaac
     5281 aagatgaaat cctttttcgc agtcaacggc aaattcagag cggcttttaa gccccaggct
     5341 atttaacaat atttcatttt cttttctttc aatttcttct tcatcgactg tttcttcttc
     5401 gacaaggggc tcttcttcag cttcaaaaag gtcttctatt aactcttctt cttttttaac
     5461 agtctttggt tcttcttcaa cttcaaattc atccaaagat attgttgaag gtatttcttc
     5521 aattgtcgct ttttcaagag ccgataaatc ttcaacttct tgtatttgtt cagcctgctc
     5581 aattttttcg gcttcttcag taagttcctc caaagaagca aggggttcaa atgttgtgga
     5641 ctcttcatct tgctgctgaa ttgaaggcag tgtttcttca aaagataatg tttccaaatc
     5701 aacttcctca aatgcggctt cttgaacttg tggttcaatt atttcttcaa tttcttgaac
     5761 ttcttctttt ccctctaaga aatcttctaa atcagtagtt tcatggactt cttcttgttt
     5821 ttcctcaata atttcttcct gttctactgg gttttcaaca gatgtttctt cgacttcaat
     5881 tagtgtttcc tgttcaggtt ttacatctgt ttctgtttct gtttctgttt cnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntctgtttct gtttctgttt ctgtttccga
     6061 ctcaagctcg gattcgggct cgggagcaac cgctccttcc atttgttcaa tctcatcaat
     6121 aggttcaata ttttctaatg gaataatatc ttcaagcggc aaaatttctt caaaagattc
     6181 ttctgcaggc gaacttgtct caggaacggt ttcaataaca ggctcccgtt ctgtgttttc
     6241 ttcaacatct tctgcttgtt tttgatgttc ttttgttaaa atatcatttg cggtatcctg
     6301 attaaaaaga gtgtcaattt cttctttttt atcggttatt atttcttctt gcggaaaaat
     6361 tatttcttct gtttcgggaa gttcatcttc ggttaaacct tcttcaataa cttcttcttc
     6421 aacaacttct tcttgtaact gttgaatatt tggcatgcct tcagaagttt cctgcacttg
     6481 tgttttttct tcttttattt cagcaacagg ctttttgcct tttttcatga aaattaaaag
     6541 caaaattaaa gggattaaaa gtgctccgcc tgcaaacatt gtcaggtttg cttttataat
     6601 attcataact ttttgaaaag gagtttcagg caggggcatt tctacctttt gaggcattgc
     6661 cggaatcggt tgagccgcag gcaaagcctg ttctgcagtt ggtgttgttg caggagctgt
     6721 ctgaggttgt ggcgcaggtg ctgtggtagt cacaggtgca ggtgttgttg caggtatgtt
     6781 tttagcgggc tgaacaacag gtttttgttc aacagatttt tgaacgggag gtttatttac
     6841 aggtttttgt accgcaggag ctgctacttt tggtttaggt ttagctacag gaattgcctg
     6901 ttttttaata acgggttttg cggtttttgc gggttgcacc gtgctttttt tagccggtgt
     6961 ggttgctttt tttaccggtt ctgctttttt agcagcagat acggccgatt tttgtgcaac
     7021 agattttttg cctgcctgcg ctataagttc tttataatca gattcactaa ctttataatc
     7081 agaagtactt aaaacctgat tttgagcaac tatttcaatc ggttttgtgg ttgaaattat
     7141 tatctttgta taaccctttg aagcttcttg gctaagttgc tgggttttaa cttctatgtt
     7201 ttgtatcaca tctgaagtcg cgctcaagga gggtttagaa gttattgaac ttacagtttc
     7261 gggcaaaaga atcacatact gaaagtcact ttttttatta accgtcagtg cttctttata
     7321 agggtttttt gtgtataagg tcactttaac acccgccggc gaagttttgt tcaagtcaac
     7381 tttaagaaga ttattgtcaa acaaatcacc gccaaaagct gcaaattgcg aaagcaacag
     7441 ggcaactcca accgacaaac cgtatttgct tttgttaaaa tcttttttca attaaaaccc
     7501 tcaatatatc ccacaaaaac attttaacat caaacatttt aaattaatta aattgtaaat
     7561 aaatttgtag ttgagataaa aggtgaaagt tttaactatt gaggacacgg aataattcca
     7621 aaatatacaa ttttgagaat tttccgtgtc cggccttaag aaggtaaaag ccaagcataa
     7681 cctcggtaaa aggatagctt ggcttttact ggactatgga ggtgcgagaa aaatttttaa
     7741 tttttccgca tcctcattta ctatttcttt tcctgttttg tcttatattg ttcagaaagt
     7801 gattttttac cggtaagcct tgtcataaag ttataatatc cttttaagaa acctgttgca
     7861 ttctcttttt tcttgtcaat ttcaataagc tcgtctctag aatgagacat tacaggcatg
     7921 cccacagatt ttcttgttaa aatttcacct attgttgtat tggaaattgt aatccaagtc
     7981 attccaagca aagaactgtt gtactgactt ctaaaggtac tattaaataa atcaatcaaa
     8041 agtgtttggt agaagagtct tgacatttct tgaacaaaac gttctttaaa tttaagtttt
     8101 gcgccttctt tgtcttcacc atttgattta agcattacca tattgtaatt atctgccatc
     8161 aagaaccaca aggttgctgc agttgcagaa gtttttgcaa ggtttgaaag atcgctgttt
     8221 gaaattccgg aaacattgca ctcattaaaa gcttttgcaa tattatcatc catgtaactt
     8281 ttgaatttaa taggggtata atttttatct tgggcggctt ttcctataaa ttctatactt
     8341 tttgaaaaag cttcaagttc tgttatttct tttaagggag gttttttacc cgagacagca
     8401 gcatatactt tgtttaaaag tttatatggc aatgttacag caccgtaggc aaacttaaac
     8461 ggtgcaatta caaaatctac taacggctta gttttctttt tatgtttaaa cagttgtatg
     8521 tattcgatat ctccgaccaa ggttttgtgt tttgccgcaa tttcctggta tttgtttgca
     8581 agcgtatcat gccctacctg cttcaatttt gaaactattt catcaaaatc ttcggctttg
     8641 attttgttat caaaattcat ggtaagcttt tcataaacct ttttaaacgg cgcgaaaatt
     8701 tgtttatcaa taaatttgcc tattgtaatc tcgggtttta cttttatgct tcttatacct
     8761 tttacagcaa atcctgccgc aataacagct aaacaagctt taaataaagt attgggactc
     8821 aaaagcggtt ctttttgttt atcctgctga gtttttataa tggctttagg ctctgtactt
     8881 tttaaagtta ctttatcttc cagtctgccc ataaaggctt tgaatacttc tctgttgttt
     8941 tgcgacttga aaaatacgtt tttataatca tgattttggc ttatctgttc ttgagattta
     9001 gcttgttctg ctgttgaaga atcttttcca tttttattaa tttcttttgc tttttctttt
     9061 gaaagcctta ctacatgctt tccgtttact atacgcgaaa tcgattcggt aaataaggtg
     9121 gttgcaaatg tcataagcat tacggcagac ttagatttgt tcatctgctt ttgaagcgca
     9181 ccgtatgtga taagcattat ataagcattt aaaactattc tgcttaattc ttgcctgaat
     9241 cttattttct tttctttatt agcatctttt ttattatcgt tacacattct tgaaaggtta
     9301 taagcatcgt ttgccaagaa tactgcggga attattcccg acacaattct gtttaaagcc
     9361 ctttcgtgtt ttgtatcata atttccggtt ttttcatcaa atatttttga aattttcttt
     9421 acactttcaa gcgaaatttc atcactgtct ttccctttaa gtttttcaaa agattcaaaa
     9481 atacctttta aggcatttac ttttgattct acctttgaat tttgtcttat ctttttaaaa
     9541 aactcggaag aataagtttt ttgcgcccat tgttttgtag ggccaaactt tcttaacaca
     9601 ccgacaacac cgtttaaaat atctttaggc aagtgtttta tcggataaat agtaccatca
     9661 gcaatcaact ccggtatcga tttttcttta ataagtaaat tttcgccttc aagctttatt
     9721 aatttttttg caacatttga atttacaaga tgttcataga gttctctaat atttttcccc
     9781 acatgtttat cggtaagctg caaaagagaa tcaacctcgg caatgcttac tttgcgaaaa
     9841 ggaccaataa aaagaccttt aaatgataaa ttcttggaat ttgaattaaa aggtctttct
     9901 tctttattat taaatatttt ttgagacaat ttagggatag aaatttcttc tcgggctctg
     9961 aacctgccct ttgaaaagct atgttctgtt tttaatttta tttccataac ccctttaacc
    10021 ttttgcaaga ttaatttatc ccacttcgaa aaattatctt acatgcttta tctttttgca
    10081 atagtacaaa attaatatct ttacttattt taatattaat tctgaaactt gccttcatat
    10141 ctagtaagta ccctaaaaaa ataaattgct gatttgtgac atttttgtta caaccgctta
    10201 actttcgacg attttggtaa caaaatgttt cacaattttt aaaataagca ttatacatgt
    10261 ataatattta agacagtttt aaaaaggaaa aatgtgaaaa aacttaaaat agcaataggc
    10321 gcagaccatg gcgggtttga acttaaagaa aaaatagctt taatgcttaa atcacaagga
    10381 tacgaaattg aagatttcgg tacatttaat tcggattctt gcgattatcc tccgattgca
    10441 aaaaaagttg caaaagaagt tgcctcaggt aattttgcca gaggaatttt aatttgcgga
    10501 accggcatag gaatgtcaat taccgcaaat aaaattaaag gaataagagc atcactttgt
    10561 catgacaagt ttagcgcgca gatgtcaaga gcgcataatg atgctaatgt actttgcatg
    10621 gggcagaggg ttattaaaga agatttggca ctggagatac ttaatatttg gctgaatacg
    10681 gattttgaag gtggaagaca caaaaaaaga atagacatta ttgaggatta gatggacaag
    10741 attgattcac aaatgcttgc aagcattatt gcgcgtattt tagacgacaa actggcaaaa
    10801 gacatttcta tactaaacat aagcaatgtt tctgttatgg cagattattt tgtaatctgc
    10861 tctgcagata cttcaacaca ggtaagagca ctttcttctt acgtcagaga aaagattaaa
    10921 atgttatttg agcgaatccc ttcaggcgaa gaaaacgact tgaaaaatcg ctggaatttg
    10981 ttggattacg gcgatgttgt cgttcatgtc ctccatagag aagaacgtga aacttatgct
    11041 atagaaaaat tttggagtca tgctctaaaa atgcccgaag aacaatggaa agaactctcg
    11101 gctgacttct cggaatatac aaggtgattt tatgaaaaga aatattgtaa aatttaacgg
    11161 cgggttggga aaccaaatgt ttcaatgggc ttttgcgcgt gcattggaaa aaattaccgg
    11221 tatgccttct tttatggata tgacttattt ttcaagaact tatgcaagac cttacgaact
    11281 tgatattttt gctgccgaaa tcaagaaaat aaagggcttt ttctctgaac taatgctgga
    11341 aacactttgg aaactgcgca gggaatttga taaaaaaagt tttttaggaa cttatttata
    11401 cgaagaacct catttcggtt ttgacaaaaa tgtttttaaa ataggaaaaa gtgcctttat
    11461 tcacggcttt ttccaaagcg aaaagtattt taagaaaatc gaaaaacaaa ttagaaaaga
    11521 ttttacgttt aaatcagagc ctgattttca aaatcaagat tttttagaga aaatcaatgc
    11581 atcaaactcg atttctcttc acataagaag aggagattac gtacaaaaga aaaaatatca
    11641 aaaactttac gcctcttgct ctttagaata ttacaaaaga ggggtggaat atatagcaca
    11701 aaaatttgaa aatccgacgc tttttatatt ttctgatgat attgactggg caaaagaaaa
    11761 tttaaaactg ccttataaaa attactttgt tgcccataat ctcgaggcaa aaagttttga
    11821 agatatgcgg ctaatgagtg cctgtaaaca caatattata gcaaacagtt ccttttcatg
    11881 gtggggggca tggctcaacc aaaaccctga aaaaattgta attgcacctc aaaagtggtt
    11941 taatgacgaa agcataaacc agtcggatat aatccctgaa aattggatta aattgggaaa
    12001 ttaaactttt atttataaat agctaaattg aaaaaatcaa aggtatgatt tttcattgcc
    12061 tctaaaacag tttgagcact gcgtacaagt ttttttaccc cgaaattttt cggattaatc
    12121 attaccgcat catacttttg gtaatcaaaa ggtaaatatc tttttacttt taatgccgta
    12181 tcctgtatcg catacaaaac tacagatttt ttacctatag attggggaag gcttaataaa
    12241 gaatcatcct ttttaaatac atctttgagc attttagtta tttcttcatg ctgtttttgt
    12301 gtaccacgaa ttaatattcc gccggtaaaa cttacattgt taattttcat attctaaatc
    12361 cttttttgta gttcactttt atatcaagtt aaaacaaaaa aaattttgct atttaaaata
    12421 ttaagtatta ataaatagtt acatttagaa aaatattctt gatataatta ttgaagtatt
    12481 ttttaaccgg aggctaaaat ggcaaagatt tctgttgtaa taaacaccct aaacgcccaa
    12541 agactcttaa aagagtgttt ggaaagtgta aaagacgctt acgaaattgt gatttgtgac
    12601 atgtactcgg aagacaaaac cattgaaata gcaaaagaat acaactgcaa aattgtttac
    12661 catgaaagaa cgggaatcgt agaacctgca agaaactggg caatggaaca agccacgggt
    12721 gattggattt taattttaga tgccgacgaa attgtcagac ccgaactttg ggaatatctg
    12781 aaaaaatatg cggataaccc taaaccgagc cactttgctt gctacctttc cagagaaacc
    12841 gcaatggacg gcaaaattct taaatcatgg tttcaaaaag gggtcaaaag gttctggaaa
    12901 aaaggaattt gtacttatac agatgctatt catcagatgc caatcacgca cgaaggcaaa
    12961 gacttttgga tagttggcaa aaatttaacc atgattcact atcacatgcc ttctataagc
    13021 tcatttaacg aaaaaataga cagatacacg gattttgagc tcaaaaggtt tgaagaaaac
    13081 gataaaaaat tctccccgtt taagctttat acccgttcct gttttgaatt ttttaaattt
    13141 tacatcctaa aaggcgggat ttttgacgga atacacgggt ttatttttgc aaaaatgcag
    13201 gcatattata aatttatcca gtgggcaaaa ctttatgaaa aagaatttaa agaaaaaaat
    13261 aaggatttgt tatattaatt atggaaaaaa ttcaaattgc actgacttac gacgataatt
    13321 acgcacagca cagcgtggtt ttgattgctt caattcttga aaacaaaacg ggaaatgaag
    13381 aaattatttt tcacattctt gacggcggct taagcgataa ttcaaaaaaa atacttcaag
    13441 acattcaagg ctgcgaaatt aaaattcatc aagtcgataa aaaacaattc gaaggatatg
    13501 ttaaatcaga ttattatcct gtaagcatgc tctggacaat gattttacct gatataatca
    13561 aggtagaaaa acttatttat ctggatagtg acatggtagt aaattcctcc ctgaaagaac
    13621 tttgggaaac agatttaagc gataactacg ttgcagcagt cgaggatgcc aacggcaaaa
    13681 aatatgcaaa aaggtacgga cttaaagatt caaagttttt taacacaggc actatgctca
    13741 taaattgcaa aaaatggctt gaagacaaca tcccccaaaa agcagttgaa attgcaatga
    13801 aaaatacagg atcaaggtgg ggctacgatc aaacagtctt aaaccagctt tttgaaggca
    13861 aagttaaatt tttggattta aaatggaact tacaatactt tccgattaat gtttgggcaa
    13921 cttacgacga caaacaagaa tataaaaatg caattaaaaa ccctttaatt attcactatg
    13981 tcggagatta caaaccctgg aaaaaaggtt taggctgctt taatcctaaa cagcaggatt
    14041 attttaaata tcataaaatt acgccatatg cttttacaga ttataacaaa tggtttcgac
    14101 aagacaaatt actgtcatac aaaggtttag ccgcttttat aaaaagatat ccgctgtttt
    14161 tccttaaaaa acagttttgg aaaaatatgt ttagtattta gttacaaatt cttaattact
    14221 ttacttaaag caaaacatga ggtaaaatca aaaaatggat agagtagagc ttgaagaaaa
    14281 aataaaagaa gccatagttg aacttgcaaa agagcctgaa aaggttgaac attaccataa
    14341 gcttgctaat ttgtatgcaa tggaaaaaaa ttatgacaaa ataatttccg tgtatgaagg
    14401 gcttttgaat gttaatccca acgacaccga tgctttgatg aatttaggaa gtttggcttt
    14461 ttacaggaaa aattatgaaa aagcgattga atattattta aaagttctag acatcaaacc
    14521 aaacgattac attttatatt ttaatatagg caacgttcat gccgaactta aaaactttag
    14581 caaagcactt tatttttata acagggctct tgattacaac ccttcaaatc ctgaaattta
    14641 caacgtaatg ggacttttat tccatgattt tgaagacttt gtcgaagcca aaatctatta
    14701 tgaaaaagct ctttctttgg atccaaacag tataaatgcc catatagatt taggaaatat
    14761 ttacttcaaa gagtccgaat acgaaaaagc gatggaacat tataaaaaag ttttggaact
    14821 ttcacctaat gacaaaactg ttgtaatttg tgttgcaaat attcttgtgg cgacaaaaaa
    14881 taaaaaagaa gctttcaaat attttgataa ggctctcaaa atggatgaaa acaactataa
    14941 gacttatgtt tgtatggcta ttgcttattc tgaattcatg gaatatgata aagccgaaga
    15001 aatatataaa ctgcttattg aaaaatatcc gaaaaacatc aacccgcata ttttattggg
    15061 taatttatat tcaaatataa aaaaacatga taaagcccta aaccaatacc aaaaagctct
    15121 tgaaatcgat cctagcaaag catctgttca tatgcttatt gcaaacacat actacatcaa
    15181 acaaaatatt gaagaagccc taacttcata tagaaaagcg attagatttg agccggacaa
    15241 cgatgaatac aagttggttt atatccaaat tttagacgaa tatattagca agaaagaaga
    15301 aaacaaatga aaaactacgg tgcccagcag cctttaatga ttgaaaaaat aatagcagga
    15361 cttagttata ttactttcgg atttgtaggc ttcatttggc ttttgatagg aatttttaca
    15421 aaaaattctc taagacctta ccttaagtat cacatttttc agtcgatttt tatttcaata
    15481 gcttattttt tggtttgtca gtttttagga ttaattttga atattctaag cataattcca
    15541 tttattaatc aaattgtaat gcaaattacg ctatatctca atatgccttt gttctttggt
    15601 ttttctataa tacaggttgc gatttatgca gttatttttt atcttgtcgc aacaagtttt
    15661 caaggaaaat acagttatat tccatggatt tcggaaataa ttaaggcaaa cgtaagaaac
    15721 agttaaatta tttctgtaat gggctctctc agaagcaact tcaaagattc acctattgta
    15781 cctgaaagtt gtttataata ttccatatcg tttttgttat tttctaattc agccaactga
    15841 taaccctttt tagaaatatt tctcatttgt ttaagcaaat ccaccgtttg tgtaatttct
    15901 ttaaacaaac ttccttcatc tcttattgtt ttatgtaaaa ccccacaata aagtcctttc
    15961 caatttcttc tggaaccgct aaatttactg tttagctccg cccattcgta taaatgtttt
    16021 acggcatctt tgtcttgagc aacttcagaa tttttgttaa gagtattaaa tttgtctaaa
    16081 taactgtcga aatcatctac aaaccaactt attttatcgt ttttatgtcc aaagaattct
    16141 gtttctttat tgtaatcatc tttttgacta agattagcta atgagcctac tacagcagct
    16201 aattcaatag gattaagcgt tgccaatttt ttatcgtaga ttaagtcgat aataggcagc
    16261 tgttgatagc catttatttt agccaaaagt tggcctttag ttgtaagttt attgtcagaa
    16321 gttatataat ccaaatgttt aagcatctct cttttatttt caaaaagctt aaacattgaa
    16381 agatattttt tctcagaggc ttttgggttt gtatcataag agtgcaggga ttttttaagt
    16441 atggttttga ttaattcgtc atcttgataa tgcttacaat atcccgcaac aaaagaaaag
    16501 tcaggattaa atgcactttc aagagaattc ggcgattcat caatcaaaat attaaaaatt
    16561 tctttttctt ctttatcttt tggtgttgta taaacatagc cgattttgtc tatacctctt
    16621 cttcctgcac ggcctgccat ttgatgaaat tcatttgccg tgatttctct tttaccgtca
    16681 tcgccatctg cagattgtgc agaagtcggt tttcttgtat ctgaaattat aactgttttt
    16741 gcaggcatat taattcctgc agaaagagtt tctgttgcta ttacaagttt taccaatttt
    16801 ttctggaata attcttctac cagctctttt tgtgtcggca aaagccctgc attatgaaga
    16861 gcatatcctt ttaacaaagc atttgtatta agagtttctc ccagatattt tccatttttt
    16921 gtatattggt ctattatttt ttgaatttct tctttttctt ttctacttgt caactgtaaa
    16981 gtcgatttat caaattcatc aagtaatttt ttgctaaatt tcctactaaa aataaataaa
    17041 atagcaggca gtctgtcttt gcccttaagc tcagaaataa atttaatata agctttttta
    17101 acatcattag cgtcttttga tttttggtgc tgatgttttt ttaacttacg accttctaca
    17161 ttttcccctg ttaaattatg agtttcaaaa acaagcggaa catgcctttt ttcaggtgga
    17221 acatcaatca aattactttt tttgcctttt acatcagaca tccaattatt aatttgatta
    17281 ttattgccta tagtcgcaga aagagataga agttgaacat ttttatctgt caaaataatt
    17341 gactgttccc aaatgccgcc cctgtcaaca tctccaaggt agtgtaattc atcaaaaact
    17401 accgttttta attttaataa attatcagga ttgtcctgaa atttctttga aaaaagcatg
    17461 ttacgataaa tttctgtagt catgactata atcggagcat cagcattgat ttttatatct
    17521 cctgttaaaa gcccaacatt atctttaccg tataaagtct gcaactccct atacttttga
    17581 ttacttagag ccttcagggg cgttgtataa aaagtttttt tgccctcaga aaggtttttt
    17641 gttattacat agtaagcaat agcggtttta ccggttcctg taggtgctgt taccaaaacg
    17701 tcttctccct tataaagctt tgctgcagcc tttttttggt attcatcagg ataacaccct
    17761 ctcggtaatt taaaataatt ttcttctaat gtttcataaa attgaggttc agttgaaaaa
    17821 ttataactgt cgcctttgac aaaagtatcc gaatgttttt gattcggcaa tttgattggt
    17881 ggggtattgc ccgcagttct ttgataagga atcctattat ttcttctttc tcccgtaaag
    17941 cttattttaa gtatttgcat tttatctcca acacgactcg cacattatta taaattccgt
    18001 catttttttt tttgctactt ggaaagaaaa ttctttacaa aactatacct tgaaaacaat
    18061 ggctattcag aataaaatgt ttatatgaaa aagatctttc ttttaatctt ggtattggga
    18121 tttttcggat ttcaagtttg cttggctgat gagcttgacg aagcaagcgg gattaatgat
    18181 gaaaacgcta tcagtaatga aagcgaaaca accaatgtgg aagaaataaa caacatattt
    18241 acaaatgatc cgcaaaacac aatggagttg cacggatatt tagaatatga cgaaattccc
    18301 gacaatacgg tttacttaga tcaggcatca aaaaaatctc ctgattttaa accgccgata
    18361 aaaatcacat caaaatcact tcttacacct tcaaataaaa aaccgaccat tccgcagtat
    18421 aaaactttgg atttggtatc aaaattttct actcaagaat atgatataaa cgaaataaat
    18481 tcaaactatt cagaaaaagc cggaagatta acttttggaa ccatgtacaa ttcagactta
    18541 gacaacgcgc aaatcaacta ttctaccggc ttttttaccc gctatgactg gaaaaaattt
    18601 gccattgcat caggttattc cagaagtacc aacagtaatt acgactctta ttacgataaa
    18661 gtttatttcg cccctgaatt taaaatcaca aaaagacttt ctattttgga tatagtccaa
    18721 agcgatgtgc aacaaatcaa gaaaaaaaat gagattgtat taagatacac cccgaattta
    18781 aaaagatatg cagatgaagt tcaatttgaa ataggtgcag gacagtactt ccgcgaagac
    18841 gaatatgtta attccgctat aagattttct acaaggttta aattgtaaaa atttttctgt
    18901 ttttttgcat aaatgtagga agtcgggtat aatgttgtca tgataaacag ttaggagctt
    18961 tatgacgaaa gaaagtgata aaagcaggaa aaaaagaaat tcaactcctg caagaaacgt
    19021 tcacagagcg aacaatccgg aaaacgaaac gcttatagat aaaactaaaa ttaaaaacaa
    19081 acaacgcaaa aacagaataa gattttatta ttctttttta acggtagttt tgcttatttg
    19141 catagcacaa ataggtttta gtgctatttt aaatatctca aaaactattt cctatcaaac
    19201 aaaaatcgca acgatgaaaa aaacaaaaaa tcaggctgaa gtgagaaaca aaaaacttaa
    19261 agaagaaatt gacaatttct cttcggcttc aagccttgaa gccattgcca gaaacaattt
    19321 aaaaatggca ggccaagacg aagttttggt tattatcaac acggcacaag aagcaaattc
    19381 tgaaaaatca gataaaaaaa ataataaaac caagaagcca aaaagcaaca agaacaaaaa
    19441 agataatcgg taaaaatgaa caatgaagtt gtatattatg tcaaaaattc ttttgagctt
    19501 aaaagccaag gacattttaa gcctgcaata gagatgcttt acaaagctct tgctattgag
    19561 ccggaaaatc tggaaatttt ggtgcagttg gctcatttat ataagctttt aggtaatttg
    19621 gaaaaatgtt tttattatgc cgaaaaagtt ttagaaatcg ataaaaatca tatcgaatgt
    19681 ttgcttttaa ttaaagatat atatatttct caagaaaaaa taaaagaagc tcttgatatt
    19741 gcagacaaaa tttttgaagc aaaacctttt aatgaaaatt tggctcaaaa aataaaactt
    19801 ttaaacatcc taaatgattc acaacaaatt gaaaaaatag aaaaaaactt aaaagatgcg
    19861 gatgacaaag ttttctttga attggcagat ttttatttta ataacaaaaa ccttgaaaaa
    19921 gcattagaaa tggcaatctc aggatatgcc aaaaatcaaa aaaataaaga tttattaatg
    19981 cttttaggca aaatttattt tgaaaaaaat gattttgaaa acgcgcaaaa atattttgaa
    20041 gaatacgcac aaaaagaaaa gacacctgaa gtcttaaatt atttaggact tttaaaatta
    20101 aattttttac aatatcaaaa agccacagaa tattttgcaa aagcttttga actcgatgaa
    20161 aaaaacagtg aatattcata taatcttgca agtgcaaatt ttttgagagg ctggtttgac
    20221 gaagctttaa aatactttac gcttgcaata tcattaaatc ctgaaagact tgactatcac
    20281 tattctttag catatttata ttatcaaaaa aaagcttatg aaaaagcttt aattgaactg
    20341 aattatattc tttcaaaaga aaattttgct atggcaacaa cccttaagtc tatgataaaa
    20401 gcaaaacaag gcaatataat tgaggcaaaa agtgatctgg aaaatatctt gaaagaaaat
    20461 cctgaagatg attttgcact gtctgctttg ggcaaaattt attcagaatt aaaaaattac
    20521 gatgccgcaa aaaatgtttt agaacaagca acattaataa aacccgaatc gcttgattat
    20581 atcagtgatt taactgaaat agaaatcgaa agcagaaatt ttgacagggc acttgaactt
    20641 gcaaacgaaa ttatcaaaaa aaatgggaaa tatgtccatg cctatgtttt gcttgcaaaa
    20701 atatatttcg ccaaaaacga atttgacaaa gtttttaatg cggcacaaga cataatcgaa
    20761 ctggattcaa acaatccaga aggttactac tacaactcgc tttcgctttt tgaacagggc
    20821 gatgtaaatt ttgcaataga aagtctgaaa aaagcaattt cacttgactt aaataattct
    20881 gccctatata ttaaaatgag cgaattttat caggaattag gagattttaa acaagcctat
    20941 gcatgggcaa aagaagcttg cgatattgac gaagcgtctt atcaaacaaa atggttatgc
    21001 gccaaattag ccgctcaact taaagaagaa aaagcagcag taaattatta ctcacacagc
    21061 tacaggcttg cttcttttga taaagatttg gtacaagaat actcgcaata tttaaaatcg
    21121 ataggaaaag aaaaacaggc aaaagcactt ttaaaataat tgaaaagtcg aaatttgtcc
    21181 ttaaaataaa taaatgttat actgttttgt acagaggata cagaaaaatt tgaaaaatga
    21241 caatttttct taatttttca aatccgacct tagaagt
//
LOCUS       DAOQAU010000029        21114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2568_length_21114_cov_6.995869, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000029 DAOQAU010000000
VERSION     DAOQAU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_21114_cov_6.995869"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1765)
                     /gene="recN"
                     /locus_tag="PKI94_05450"
     CDS             complement(86..1765)
                     /gene="recN"
                     /locus_tag="PKI94_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNW26222.1"
                     /translation="MIKQILIKDYILIDELLVNFDNGLNVITGETGAGKSIIINAIDL
                     VFAPRVSKDVIKTGCEKALIELVLDYSKQDLNQIFEENGIDNFGNEIVISKEISENAV
                     RSRVNGTLVNQEFIKILKDKFLDIHSQHQTYAFLQPKFHINLLDAYAKDSYGNDLNEY
                     KIIFKQYEKLCNELESAKSLSNATENQIEFLKFQIKEIEDAQINDENEYEILKNELNV
                     LENAEKLKELTGTSYWSMNGDENSILGSLGLIKMNISKAAQMDGNLAFVEGNLISAAE
                     VLSEISAALRDYSQNLENDTEKLNTIQERIFFLDKIKRKYGNTLSEVMQTLTRLRQEY
                     DQIEFSTQNIEELELKISELREELSTKASVLSENRKYFAQSLSASIVEKLENLELPKV
                     RFEILVEELHEGVNAGINSNGCDRVEFLISTNVSEDLKPLAKVASGGEISRVMLAIKT
                     IFAQNDDIDTVIFDEIDTGISGKTAQSVADEISELAKYHQIILITHQAIIASKANKHF
                     YVTKSQDNDITSVLVYTLEGDNRVKALAELAGGEINDQSIDFARSLLYQPA"
     gene            complement(1783..2529)
                     /locus_tag="PKI94_05455"
     CDS             complement(1783..2529)
                     /locus_tag="PKI94_05455"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNW26223.1"
                     /translation="MLDALEKILAVKKISYESFSIDCPKHGFDFVFVIGGDGTLLRAA
                     RFFAKEKTPVFGFNLGKLGFLAQSKVSELEKTVKKILNYEFKTEDRLMLVSNNDILAL
                     NEFVIRGTSATKTSRFCLSINDKFVCEYLADGLIISTPTGSTAYGLSAGGAILHPEIE
                     AISIVPICPHTLTARPLIIPANEMIKVSTCDVNPNLTIISDGQGTFDNITEIKIQKCN
                     FKAKLAFLEENDFYSVLREKLQWGVAPKST"
     gene            2759..3427
                     /locus_tag="PKI94_05460"
     CDS             2759..3427
                     /locus_tag="PKI94_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNW26224.1"
                     /translation="MKIIPIIQQYNLQKTRNKYIDVNFQSSKFYNHKICLPKTGICNF
                     KQWDENLYSGSQPGKRGDFGDIYFLDTNSLKNDLNILKQTGIDKIIDLRRETTDGICV
                     SAEREKTKKMAMEYKNIPMSAKNIPTHEQIKEFFNELKTAKGRVFLHCKEGYDRTGMM
                     LCIYLAHITGDKPNKIVNKVFEQRNLIPFRYLNDVNKQKMLSLMSYISSMNKRDFEYF
                     LKTR"
     gene            3565..4641
                     /locus_tag="PKI94_05465"
     CDS             3565..4641
                     /locus_tag="PKI94_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26225.1"
                     /translation="MTAPVNNVKKTQGINFDINNPFLQNTQSIPEYMTMNGSIFNAPQ
                     ATSSSSEENDTQEKTKEGEKKKESWLNKTFGAEKKAKGETSNCKDISKDSKTFVQDFD
                     KQNKNVQNNTKKAIQLFTKNDKEIAKTTKDIQKDQAEVADLTAEKASLEAEIESASNT
                     DSTGSGEGSAFSLNLAGTKDDKESAKTSTDSGKTSELQQELASVEGKINTAQTSIKTN
                     TKQITTITKQSMSFKNNVNKLITEAMKYTVKTQNTIAAGQQKANTANTIGQVVTITGG
                     AATAVGGAMELIPGCQAAGAVVMKAGIVTTAAGGVTTSTAQLASGDLAGAAQSATNAA
                     NQATTAANTIKNYKAEPTEPTKTK"
     gene            complement(4698..5312)
                     /locus_tag="PKI94_05470"
     CDS             complement(4698..5312)
                     /locus_tag="PKI94_05470"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNW26226.1"
                     /translation="MEKRFSDKVINRLTLYHCILSDYMKKGIEFISSPQIASLLNIDD
                     SQVRKDIGLLKNLGKCRVGYSVAELKETIEKTLGFKKVKNAFIIGAGNLGTALANYGN
                     FINYGLNILALFDNDKSKVGSKINNKEVFDISDLPELAKKMSVDIAILTVPGEYAQNT
                     ADFLVKSGLKYIWNFSPTVLSVPKNVQTWNENLMGNFLQFTYRI"
     gene            5417..6667
                     /locus_tag="PKI94_05475"
     CDS             5417..6667
                     /locus_tag="PKI94_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNW26227.1"
                     /translation="MKPELLMPAGNLEKLEYAIKYGADAVYLGVVDFSLRSMRKGELI
                     TFDNLKQAIDLAHSLGAKAYLTLNIFAFNNDIENLEKSMPVIKETNPDAIILSDLGVF
                     KIVKKYMPDVPIHVSTQTNTLNYEAVKFWQDLGAKRVILARELPISDVAQIRKKVPDI
                     ELEVFVHGSQCMSFSGRCLLSDYMTDNERKANQGNCSQPCRWEYALVERTRPDQQFEI
                     HQTERGAHILSPKDLCLVEHLQKLIDAGVNSFKVEGRTKSLYYVSAVAKAYRNAIDEV
                     VKNPQADLSKYFEELKKVGNRGYTQGFYLGKHDENCYNYETSKGLAGADFLCEFWEKA
                     QDGFYKVKIKNKILLNDKVEIITPDEQFLSKVVEIKDKDGVKVNIANTNDELFLKFSK
                     EPKDYKYALARTIDVLYINKKVKQ"
     gene            6676..7155
                     /locus_tag="PKI94_05480"
     CDS             6676..7155
                     /locus_tag="PKI94_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HNW26228.1"
                     /translation="MLLKPDYNLKNIYEIDLEALKQQGIKAIFFDLDSTIMVSKSGNY
                     HEETEQWLKKVQENFFIAVVTNNKKFEYVEKVKSISSFQVIADAKKPSPKAMKKLLEK
                     INISPKEVVIVGDRPLTDILAGKLLGAKTILVDSINAHNEGLPTRFIRWLERRFILN"
     gene            complement(7147..9507)
                     /gene="recJ"
                     /locus_tag="PKI94_05485"
     CDS             complement(7147..9507)
                     /gene="recJ"
                     /locus_tag="PKI94_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNW26229.1"
                     /translation="MKKEWIVNKDSKLKNKPLLERLLFSRGVKTQVEIKEFLNPLELK
                     LTHPNVFCDMQNAVDRISKAVDNQENILIYGDFDADGVTSTSLLLRTFRHLGAKVDYF
                     IPDRDLEGHGLNSKALVKLLATKKPKLIITVDCAVSDVEQVQFLKSFKVDVIITDHHE
                     APEILPEALAIINPKAQNALNATLSAKEIKYLTSLAGVGVAFKLAQAILEHYKKLDFV
                     YDLLPYVTVGTIADIVPLIGENRYFVTKGLELISNGKHYGLKRLLEVSGNWNDTGITA
                     DRIAFGVAPRINASGRLDTVENALKVLTSDNKQEIEISIIALDNFNKVRQKLCEDTFL
                     EAQEMFKTQGNSDNAIILFNPKWHVGIVGIVASKFVEKYYKPAFLMTFSEETKQIRCS
                     SRSIEGVDVYDVLSANAELFEGFGGHSMAGGFAFDSQKISFEEVKTALNKTINEIVGD
                     KKLLPTLHVDMELEPGEISGNLIADIERLQPFGAENPNPIFVLNNLTVLQKKLMGADR
                     NHLKLTVGDTQNNTYDCIWWQKGDVSLNQGDKLDIAFYPQTNTYNGNTSIQLILEDVH
                     SESLVEEQVFEQVQNPSQIKYYDHRKKTNIHANVEDYVRNSDLKISVFAEDKAVINAL
                     KPYNALSERIFNRQTLGKCDALMFFDYPPSEKVFTEIISKASPKFLHFMNYDVKKIDE
                     KELLKTFSGMLRYSFKNQQGVFNLEKSSVFLAMDENIITFMIQTFEEAGLLQILDKSE
                     KDYKIELFDVSDITKTLHTKKYKEFKTLLEESQEYKTALFEGEISV"
     gene            complement(9622..10839)
                     /locus_tag="PKI94_05490"
     CDS             complement(9622..10839)
                     /locus_tag="PKI94_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNW26230.1"
                     /translation="MDIKNGFKKRFAAFGLVFFGIFAFGLSAFSYQSAELYDNVWKLI
                     NTKYVDQTNNMQDWNTWRHKYDNYIKTDEDAYVAINTMVASLDDPYTKFLDPKEFAEE
                     TSSIKGSLKGIGIQIAVRDGKLMVIAPIESSPAEKAGLLAEDEILEIDGVSTKGMAID
                     KAADKIRGEEGTTVTLLVKRKEDIKPYTIARKEIEIKSVSTKIPNDVILPKNVGYIRL
                     SSFISKNTSKEFETILVQSKDKDGFIIDLRSNPGGLLSNAIFISNLLLPNGIIVSTVD
                     RDGYKETQRTMGGCVTKKPVVVLINKGSASASEILSGALKDNKRAVIVGEKSFGKGLV
                     QEINRLPNNSGINITIQKYLTPNGTDINKKGIDPDILVELSDDDIKNKDDKQLKKANE
                     VLVDLINKSSCCK"
     gene            11010..11138
                     /locus_tag="PKI94_05495"
     CDS             11010..11138
                     /locus_tag="PKI94_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26231.1"
                     /translation="MHEYVFKMKKGEIEIELKSDDIEFVEQQLNKWREDIMKNNSK"
     gene            11161..11868
                     /locus_tag="PKI94_05500"
     CDS             11161..11868
                     /locus_tag="PKI94_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26232.1"
                     /translation="MKYSFTFKKGDIFVEFTTSDKLVVEKQFKFWVDAASDYSRKHPS
                     AKPAKENPKAPLKEEKVSTAKPQKQVETKSVKNKKEEAQEEPKEVFDKASTLLKTINL
                     IQNPKLPKEEKPKSEPDFEKVLEKTIEKPSFEPSKKHDKTFLELVNSKESQDKLHHFI
                     TAAYYLSEYEKTDRFTLKQVNSKLMQNLSEVIDHSVLQDSIRQELIEVVPDYTGTSEV
                     CEYTLTPKGEEFFTNLK"
     gene            11957..13126
                     /locus_tag="PKI94_05505"
     CDS             11957..13126
                     /locus_tag="PKI94_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW26233.1"
                     /translation="MKKKIIIISAIIAAAVVARFVFSLYGQIMMGKMMAKTATPSVVL
                     GEIQTADVLKQVEAPGRILSKYRIDVLARIDGYLTKSYFKEGDHVKKGQVLFEIEPEE
                     WYLAVKKARANVANTKAQLIYAEKQLKRGEELVKQDYIAKAAYDNLLSQRDALSAQLT
                     LNQATLSDALRNYGYTKVKAPVDGQVGMITVTVGNYVNPSAGALTTIYSNNPIYVTFP
                     IDSKEYDILTKIDAENVVRKVDLFFPTGEKYEIQGNQDFHDNKVDETTGTVTMRATFE
                     NPKSRLLNGDFVKVIVFSKNTINVPIVPQTAVLENPQGKYVYAVDEKNIPHIVQIKIS
                     GQYKDNWIVTEGLKKSDIIVVDGIQKVIPDRPVKIVDKETMEKIKKHQKPEEGKK"
     gene            13130..>14430
                     /locus_tag="PKI94_05510"
     CDS             13130..>14430
                     /locus_tag="PKI94_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW26234.1"
                     /translation="MDTNLFIRRPKFAIVISLVIVLAGLICMNTLPLEEYPMITPPQV
                     VVSASYPGASSDVVEATVAAPLEAQINGVENMIYMTSSSQNDGYSLNVFFKIGTDPDM
                     AVINIQNRLQLVTPRLPEDVRRYGLMVKKTVGGPGLLMISVNSPNNTYNPLFIANYAS
                     IYVKDEIARIPGVSDVNVFGSADYSMRIWLNATKMAALGISTSDVYSAIQQQNTQVPA
                     GDIGIEPLAEKQMVKLTLRTHGRLKNADEFENIIVRSRPDGSQVKVKDIAKVELGAES
                     YSFFSRIQGRNTAMIMVKQLPEANSIALAKTIRKRLAQLSKTFPPGLEYHVDRDETEF
                     VQESLNEVVQSMFLAIGLVLIVTYVFLSSMRAAMIPFFAIPVSLIGTFIFIDLFGFSI
                     NLLTLFGMVLAVGLVVDDAIVVLENVQRHIQEGVNPKEATE"
     assembly_gap    14431..14530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14531..16090
                     /locus_tag="PKI94_05515"
     CDS             <14531..16090
                     /locus_tag="PKI94_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW26235.1"
                     /translation="EVRNKYLTSAKYFVDNTKNTMILFGIFILITALLFKIIPTSFLP
                     EEDKGAIFTQIQLPDGSSASRTDIITREVEKRIQTYKGVKETISLVGFAGENTALVVT
                     ILDNWSKRHGSKLSANGIIGKIRQDFGNYPSAVIVSFSPPSIPGLGMFGGFEYQLLDK
                     GDRSPQDLYLESMKFVADANKNPALSSVYTQYTANLPQLMFDVDTAKAMAQGVTIQEI
                     YTTLAANFGQTYVNDFNKYGRVFRVMMMAQDEFRAKRQDIDKIFVKNINGKMVPLTSV
                     IKLKDVVGPYTITRFNMYKAVTMNGSAAKGVSSGKAMKTMEDLSNKILPKDMGYAWSG
                     ISLQEQESSGQIGFILATALIFVYLFLVALYESWTLPLAVMLISPIAIMGGLLFQYVF
                     GYSLDIYSQIGLVMLIGLSTKQAILIIEFAKEAKENLKMHYNDAAMEAAKLRFRAVMM
                     TAIAFILGMLPLVFASGAGSTSRRSIGMTVFGGMIAVAFIGTMLVPAFFVAVEKMKDN
                     VEEFILKRKQQ"
     gene            16087..17559
                     /locus_tag="PKI94_05520"
     CDS             16087..17559
                     /locus_tag="PKI94_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW26236.1"
                     /translation="MKSIKKLILILIIFTFSILPTLAFGLKATKSEKILDETLGNSIV
                     KEEYPAEAQVSPVETDADKQEKNLVIEGSVQKTIDITLGECIKLALGNNPRIKAALNE
                     ALASHARISQAWSNYFPELDWQSGYTKTRNLQTATMIGNTSAFNYYLLGQVSVSQMLY
                     DFGVTQNTVTIKKLDYEAYKTSLTAVVNDVICQTKDAYYNLLFAYENRNVAQDTVQKY
                     EMFYNQAKAFYEIGMNPKVDVTIAEVNLSNAKLNLIQAENSIDLAVATLNNVMGVPYI
                     NKYNIQERLKFNPITMSMNKAFELAKESRPELKLAEIKVQEANQAVKLAKKAYFPTLE
                     ASGSYARGGEQINETYGYNYGVYLNFPTVNGMLIKNQIKEARSLYDREIANAQITKNN
                     IYLEIQQAYLMLYEKKNQLPVAFLQVKQAKENYDLSFGRYKVGVGNPTELKDAQNSYQ
                     SAQLNYYKALYEYNSAKAKLEKAIGKNIIADDDESIDLET"
     gene            17574..18902
                     /gene="lysA"
                     /locus_tag="PKI94_05525"
     CDS             17574..18902
                     /gene="lysA"
                     /locus_tag="PKI94_05525"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNW26237.1"
                     /translation="MLSTNQYIKPLTTCINENGNLSIGGCDLVDLAQKYETPLYVIDE
                     KTLRTICKEYKQAFSSYERVKMLYASKALMTKAVAKIVNSEGFGFDAVSGGELYTINK
                     TGIDMSCVLFNGNNKSYDELDMALELGVGRISVDNFLELSLLNEIAKKHNKKVDILLR
                     ITPGIECHTHEYIQTGNLDSKFGFDLSQIHDAIELVLEQYPNINLKGFHTHVGSQIFE
                     TQIFEDEIDILIKEIVYVKEKFNILLDEINIGGGLGVKYIPTDLPPSVFEIGELVINA
                     LNKSIKKHNIPAPLLYLEPGRSIISTAGVTLYTVGSSKQVPNGTLYWSVDGGMADNPR
                     PSMYQAEYTAQIANKPEQDCSQKVTIAGKFCESGDILIKDINLPELEEGDILCVFNTG
                     AYNYSMASNYNRFQKPAAALVNNSQSDMIIIRESLDDLLSHDVLPARLDK"
     gene            18941..19822
                     /gene="cdaA"
                     /locus_tag="PKI94_05530"
     CDS             18941..19822
                     /gene="cdaA"
                     /locus_tag="PKI94_05530"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNW26238.1"
                     /translation="MNAIKWSLNWLDLVEVFAIFIMLFAFYRRFIKNTQSEKLVKGMV
                     FLLIAWVTSEILISLGLQILGVFIKSMVTLVALSLIVVFQPELRRFLGYLGQPGFISR
                     GFWKNSNNKESINVVKEVIEAVKYLSKSRIGALIVFQKEMNASTYFDVGTKLNADVTT
                     ELLLTIFHPNTPLHDGAVVIKNDKILSAGVLLPLTEDPKLSWKYGTRHRAAIGTSETS
                     DSGCLVVSEETGDVSMSVDGVLKKYDDITTLKTDLETFLGYADYDEIHKKTIFKINNL
                     INIKKAEIMPKVNKDKV"
     gene            19832..20710
                     /locus_tag="PKI94_05535"
     CDS             19832..20710
                     /locus_tag="PKI94_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNW26239.1"
                     /translation="MLVKKTNKESSFISWIVKLILLTTGAFIAGFGLEGFLIPNSMID
                     GGIVGISIMLSYLTKINLGILLFCLNLPFIFLALKKLGRIFVVQTAYAITMLSLSVNV
                     FHKMHVTDDLLLATVFGGVVLGLGVGLILRSNAALDGTEILAIKLAKKAGFSIGEIIM
                     FFNFFIYTAAGFLFGWDRAMYSILVYFIAYKVIDIVLEGLNESKSVTIISNRAEKIGE
                     EIIKRLDVGVTYLSGKGGYTGEDKKIIYCVISRLELVKLKELVNLIDKQSFIAIENVH
                     EVEGVRIKQKRAGKKK"
     gene            20783..>21114
                     /locus_tag="PKI94_05540"
     CDS             20783..>21114
                     /locus_tag="PKI94_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26240.1"
                     /translation="MAKHIDTIPIEVSILTVDHDIKGTVHVSKYTSTNRELTDLLNDR
                     ERRFLAVTNAEIYSRNSTQTPRRYNFLEIHMDYVLMVHPSTQAVFKESGKAQEDIARF
                     RELRQKLN"
BASE COUNT         7111 a         3664 c         3750 g         6489 t
ORIGIN      
        1 gaaaaaaatt gttatttttt aacaaaatgt tacaggaaag atacaaaaat cgcccgattg
       61 gggagtatgt aattttttat atttattagg cgggttgata taaaagcgag cgggcaaagt
      121 ctatagattg gtcgttgatt tcgccacctg caagctctgc aagtgctttt acacgattat
      181 caccttcaag ggtgtaaact aaaacggaag ttatatcgtt atcctgagat tttgtaacgt
      241 aaaaatgttt gttagccttt gatgctataa ttgcctgatg cgtaattaaa attatctgat
      301 ggtattttgc aagttctgaa atttcatcag caacgctttg tgctgtttta ccggaaatgc
      361 ccgtatcgat ttcgtcaaaa atcaccgtat ctatatcgtc attttgggca aaaatggttt
      421 ttatcgcaag cataactcgc gaaatttcgc ctcctgatgc cacttttgca agcggtttta
      481 aatcttccga aacatttgtg gaaattaaaa attctaccct gtcgcaaccg tttgagttta
      541 tccccgcatt taccccttca tgtaattctt caaccaaaat ttcaaaacgg actttgggaa
      601 gctccagatt ttcaagcttt tcaacgattg atgctgaaag cgactgcgca aaatattttc
      661 tgttttcaga aagcacagaa gcttttgtgc ttaattcttc acgaagttcg ctgattttaa
      721 gttcaagctc ttcaatattt tgtgtcgaaa attcaatttg atcatactct tgtcttagcc
      781 gtgtcaaagt ctgcataacc tctgatagag tatttccgta ttttcttttt atcttgtcaa
      841 gaaagaaaat acgctcctga atggtgttaa gtttttctgt gtcgttttcc aaattttgcg
      901 aataatctct gagcgcggct gaaatttcag ataaaacttc agccgcactg attaaatttc
      961 cctcgacaaa cgctaaattt ccgtccatct gagctgcctt ggatatattc atttttataa
     1021 gacccaacga gcctaaaata gaattttcat ctccattcat tgaccaataa gaagttcccg
     1081 tgagttcttt taatttttct gcattttcaa gaacgttaag ttcatttttt aaaatttcgt
     1141 attcattttc gtcgttgatt tgagcgtctt cgatttcttt tatctgaaat tttaaaaatt
     1201 caatctgatt ttctgtagca tttgaaagcg attttgcgct ttcaagttca ttacaaagct
     1261 tttcatactg tttaaaaatt attttatatt cgtttaaatc attgccgtaa gagtcttttg
     1321 cataagcatc aagaagattt atgtgaaact tcggctgcaa aaaggcgtaa gtctggtgct
     1381 gagaatgaat atccaaaaat ttatctttta aaattttaat gaattcctga tttaccaaag
     1441 ttccgttaac ccttgaacgg acagcatttt cggaaatttc tttggaaata acaatttcat
     1501 ttccaaaatt atctataccg ttttcttcaa agatttgatt taagtcctgt ttggaataat
     1561 ctaagacaag ttctatcaaa gctttttcac atccggtttt aataacatct ttgctaaccc
     1621 ttggggcaaa aacaaggtca atggcattaa taataatgct tttgcctgcg cccgtttcgc
     1681 cggtaatcac atttaatcca ttgtcaaaat tcacaagaag ttcatctata agaatataat
     1741 ctttaattaa aatctgttta atcatcccgg actccctttt ttttaagtgc ttttaggggc
     1801 aacaccccac tgaagttttt ctctgagaac agagtaaaaa tcgttctctt ccaaaaaagc
     1861 aagttttgcc ttaaaattac atttttgaat ttttatttca gtaatattat caaaagtgcc
     1921 ctgcccgtct gaaataattg ttaaattagg attaacatca caagttgaaa cctttatcat
     1981 ttcatttgca ggaataatca ggggtcttgc ggtcaaagtg tgaggacaaa tcggtacaat
     2041 cgatattgct tcgatttcag ggtgtaaaat agccccgcct gcagaaagcc cataagcagt
     2101 tgaacctgtg ggcgttgaaa taataagtcc gtctgctaaa tattcacaaa caaatttgtc
     2161 attaatactt aggcaaaacc ttgaagtttt tgtggcagaa gtgcctctta taacaaattc
     2221 gttcaaggca agaatgtcgt tgtttgaaac aagcatcagc ctgtcttctg ttttaaattc
     2281 gtaattgaga atttttttaa ccgttttttc taattctgaa acttttgact gagcaagaaa
     2341 tcccaatttg cctaaattaa aaccaaaaac aggtgttttt tcttttgcaa aaaaccttgc
     2401 agccctaagc aatgttccgt cgccgcctat aacaaacaca aaatcaaatc cgtgttttgg
     2461 acagtctata gaaaaacttt cgtaagaaat tttttttaca gccaaaatct tttctaaagc
     2521 atcaagcacc ttttgcgctt cgcttatctg cggtttaaaa actatagcaa cttttttttc
     2581 tgaaaaattt tccacttaag cccctttgtc ccctttgtcc ctttggtttt tatattctac
     2641 catattttaa atgttttggc atattttttt tattattaat atttgtaaat tttttatttt
     2701 taaagaataa aaaaaaagtt tttcgaactt ataaaaatat caataaaaag gcttttatat
     2761 gaaaataatc ccgataattc aacaatataa tctccaaaaa acgcgcaata aatatattga
     2821 cgtcaatttt caatcttcaa aattttataa tcacaaaata tgtttaccaa aaaccggcat
     2881 atgtaatttt aaacagtggg acgaaaatct ttattcgggc tctcaacctg gtaaaagagg
     2941 agattttgga gatatatatt ttttggatac aaattctcta aaaaacgatt taaacatttt
     3001 aaaacaaaca ggaattgata aaataatcga cttgagaaga gaaacaacag acggtatttg
     3061 tgtcagtgca gaaagagaaa aaaccaaaaa aatggcaatg gaatacaaaa atattcccat
     3121 gagcgcaaaa aatatcccta cccatgaaca aataaaagaa ttttttaatg aattaaaaac
     3181 agccaaaggt cgtgtatttt tacattgcaa agaaggctat gacagaacag gaatgatgtt
     3241 gtgcatttat ttggctcata ttacgggaga taaaccgaac aaaattgtaa ataaggtttt
     3301 tgaacaaaga aatttaatcc ctttcagata tttaaatgat gtcaataaac aaaaaatgtt
     3361 aagtttaatg tcatacattt caagcatgaa caaaagagat tttgaatatt ttttaaaaac
     3421 gaggtaatta gttttaaaac gtgaaattca gccctatttt taaagttatt ttgtaacgat
     3481 atgtaacaat ttgtatatac caagcaattt aaaaagtata tactacttta tatatataag
     3541 agtttaaaaa taggagagca tattatgaca gcacctgtaa ataacgttaa aaagacacaa
     3601 ggtataaatt ttgatataaa caatccattt ttacaaaata cgcaaagtat cccggaatat
     3661 atgactatga acggaagcat ttttaatgca ccccaagcca catcctcatc ttctgaagaa
     3721 aacgatactc aggaaaaaac caaagaaggc gagaaaaaga aagaatcttg gctgaataaa
     3781 acctttggtg ctgaaaaaaa agcaaaaggt gaaacctcaa actgtaaaga tataagcaaa
     3841 gactcaaaaa cttttgttca agattttgat aaacaaaata aaaatgtcca aaacaataca
     3901 aaaaaagcaa tacaattatt tacaaaaaat gataaagaaa ttgcaaaaac aacaaaagac
     3961 attcaaaaag atcaagctga agttgcagat ttgacagcag aaaaagcatc tcttgaggca
     4021 gaaattgaat cagcatcaaa tacagattca acaggcagcg gagaaggaag tgctttttct
     4081 ttaaatcttg ccggtacaaa agatgataag gaaagtgcaa aaacttctac cgattcaggt
     4141 aaaacttcag aactacaaca agaattagca agcgttgaag gaaaaatcaa caccgcccaa
     4201 accagtatta aaacaaacac caaacaaata accactatca caaaacaatc aatgtctttt
     4261 aaaaacaatg tcaacaaatt aataacagaa gcaatgaaat ataccgtaaa aacacaaaac
     4321 actatcgccg cgggccaaca aaaagctaat actgctaaca caatcggaca agtagtaact
     4381 ataacaggcg gtgcggcaac agctgtcggt ggagcaatgg aattaatccc cggatgtcaa
     4441 gcagcgggag ctgtggtaat gaaagccgga atagtaacaa ctgcagccgg cggagttaca
     4501 acatctactg cgcaattggc aagcggcgat ttggcaggtg ctgcacaatc agcaactaat
     4561 gcggctaacc aagcaactac agctgcaaat acaattaaaa actataaggc tgaacccact
     4621 gaacccacta aaaccaaata aaataattat aaaaaaccaa gagggaaaaa ttcttaagaa
     4681 tttttccctc ttggttttta tattctataa gtaaactgca agaaatttcc catcaggttc
     4741 tcgttccagg tttgaacatt ttttggaacc gaaagcactg tgggagaaaa attccagatg
     4801 tatttcaatc ctgatttcac caagaaatca gcagtgtttt gtgcatattc cccggggacg
     4861 gttaaaattg ctatgtcaac ggacattttt tttgcaagtt cgggtaagtc tgaaatgtca
     4921 aaaacttcct tgttattaat ttttgagccg acttttgatt tatcattgtc aaaaagcgca
     4981 aggatattca atccgtaatt aataaaattt ccgtaatttg caagtgctgt tcccaagttt
     5041 cccgccccta tgataaatgc gtttttgact tttttaaagc cgagagtctt ttcgatggtt
     5101 tctttaagtt cagccacaga gtatccgaca cggcatttgc ccaaattttt caataaacct
     5161 atgtcttttc ttacctgtga atcatctata tttaaaaggc ttgctatttg cgggcttgag
     5221 ataaattcaa ttcccttttt catataatcg gaaagaatac aatggtataa agttaatcgg
     5281 tttataacct tatcagaaaa tcttttttcc attaaaaaat cctaaaaaat gtcgaaacta
     5341 taaaaaatta tacataaaaa tcactttatt tgaaagattt cttgattaga gggcgttttt
     5401 ttgtttaaat caaattatga aacccgaatt attaatgcct gcgggcaact tggaaaaatt
     5461 ggaatacgcc ataaaatacg gcgctgatgc cgtttatttg ggcgttgttg atttcagcct
     5521 gcgctcaatg cgaaaaggcg aattgataac ttttgacaac ttgaaacagg cgattgattt
     5581 ggcacattcg ctgggtgcaa aagcttatct gacacttaac atctttgcat tcaacaacga
     5641 tattgaaaat cttgaaaaat ctatgcctgt aataaaagaa accaaccccg atgccattat
     5701 acttagtgat ttaggggttt tcaagattgt aaaaaaatat atgcctgatg tgccgattca
     5761 tgtcagcact caaacaaata cactaaatta tgaagcggta aaattttggc aagacttggg
     5821 tgcaaaaagg gttattttgg cgcgagagct tcctatttca gatgttgccc aaattcgcaa
     5881 aaaagtccca gatatcgagc ttgaagtttt tgttcacggc tcgcaatgca tgtctttttc
     5941 gggaagatgc ctattgagtg attatatgac cgataacgaa cgaaaagcca atcaaggcaa
     6001 ttgttctcag ccttgcaggt gggaatacgc tcttgttgaa agaacccgcc ccgaccaaca
     6061 atttgaaatt caccagaccg aaaggggcgc acatattttg agccctaagg atttatgtct
     6121 tgttgaacat ttgcaaaaat tgattgatgc cggagttaac tcttttaagg tagaaggcag
     6181 aaccaaaagt ctttattatg tttcagctgt cgcaaaagct tacagaaatg caatagatga
     6241 agtcgttaaa aacccccaag ctgatttgag caaatatttt gaagaattaa aaaaagtcgg
     6301 aaacagaggc tatacgcaag gattttatct gggcaaacac gacgaaaact gctacaatta
     6361 cgaaacttca aaagggttag caggagctga ttttctttgt gaattttggg aaaaagcaca
     6421 agacggattt tacaaagtaa aaataaaaaa caaaattttg ttaaacgata aagttgaaat
     6481 aattacgccc gatgagcaat ttttgtcaaa agtagttgaa attaaagaca aagatggtgt
     6541 aaaagtaaat atcgcaaaca caaatgacga acttttctta aaattttcca aagagcccaa
     6601 agattataaa tacgcacttg caagaacgat tgatgtcctt tatattaata aaaaagttaa
     6661 acaataagga gttttatgct gttaaaaccg gattacaatt taaaaaacat atatgaaatt
     6721 gaccttgaag ccctgaaaca acagggtatc aaggcaattt tctttgattt ggacagtacg
     6781 attatggttt caaaatcagg gaattatcac gaagaaaccg agcaatggct taaaaaagtt
     6841 caagaaaatt tttttatcgc agttgtcaca aacaacaaaa aatttgaata tgtggaaaaa
     6901 gttaaaagca tttcaagctt tcaagtgata gcagatgcca aaaagccctc gcccaaggct
     6961 atgaagaaat tgctggaaaa aataaacata agccccaaag aagtagtaat agttggcgac
     7021 aggcctttaa ccgacattct tgcaggaaag cttttgggcg caaaaactat tcttgtcgat
     7081 tcaataaacg cccacaacga agggcttcct acccgtttta tccgctggct tgaaagaaga
     7141 tttattttaa actgaaattt cgccttcaaa aagtgctgtt ttgtactctt gtgactcttc
     7201 aagcagggtt ttaaattctt tatatttttt tgtatgaagg gtttttgtaa tgtctgatac
     7261 gtcaaaaagt tctattttat aatctttttc gcttttgtca aggatttgaa gaagtcctgc
     7321 ttcttcaaag gtttgaatca taaaagttat tatgttttca tccattgcga gaaaaacgga
     7381 acttttttca aggttaaaca ccccttgttg atttttgaaa gaatatctta acatccctga
     7441 aaaggttttt aaaagctctt tttcatcgat ttttttcaca tcataattca taaaatgcaa
     7501 aaatttgggc gaagcttttg aaataatttc tgtaaagact ttttctgaag gcggataatc
     7561 aaaaaacatc aaagcgtcgc atttgccgag cgtttgacga ttaaaaattc tttcagacaa
     7621 agcattataa ggcttaagcg catttataac cgctttatct tctgcaaaaa cagaaatttt
     7681 taaatcagaa tttctgacat aatcttcaac atttgcatga atgtttgttt tttttctgtg
     7741 atcgtaatac ttaatttgag aaggattttg aacctgttca aaaacttgtt cttcgacaag
     7801 gctttcggaa tgaacatctt ctaaaatcag ttgtattgaa gtatttccgt tataagtatt
     7861 ggtttgtggg taaaaggcaa tatcaagttt gtcgccctgg ttaagagaaa catctccttt
     7921 ttgccaccaa atgcagtcgt aagtgttatt ttgagtgtcg ccgactgtga gtttaagatg
     7981 gtttctgtct gcgcccatta attttttctg caatacagtc aaattattca gaacaaaaat
     8041 cggattgggg ttttccgccc cgaacggttg taagcgttca atgtctgcaa ttaagtttcc
     8101 gcttatttcg cccggttcaa gttccatatc aacatggagc gtcggaagaa gttttttatc
     8161 accgacaatt tcgttgatcg ttttgttaag cgcggtttta acttcttcaa aagaaatttt
     8221 ttggctgtca aaggcaaatc cgcctgccat tgaatgaccg ccaaagcctt caaaaagctc
     8281 agcatttgcc gataaaacat cataaacatc taccccttca atacttcttg aagaacatct
     8341 tatctgtttt gtctcttcgc tgaaagtcat taaaaacgca ggtttatagt atttttctac
     8401 aaattttgag gcgacaattc ctacaattcc tacatgccat ttggggttaa aaagtattat
     8461 tgcattatcc gaattgcctt gtgttttgaa catctcttgg gcttctaaaa aagtgtcctc
     8521 gcacagtttt tggcgaactt tattgaaatt gtcaagggcg attattgata tttcaatttc
     8581 ttgtttattg tctgaagtta aaactttaag tgcgttttcc accgtatcaa gtcttcctga
     8641 tgcattaatt ctgggggcta caccgaaagc aattctgtct gcggttattc ctgtgtcgtt
     8701 ccaattgcct gaaacctcca acagcctctt taagccgtag tgttttccgt ttgaaatcaa
     8761 ctcaagacct ttagtaacaa aatatctgtt ttcaccaatt aacggaacaa tatcggcgat
     8821 tgtgcccact gttacatagg gcaataagtc atatacaaaa tcaagttttt tatagtgttc
     8881 caaaattgcc tgcgccagtt taaaggcaac gcctacacct gcaagcgacg tcaaatattt
     8941 gatttctttt gccgataatg tagcattaag ggcattttga gccttaggat taattatcgc
     9001 aagggcttcg ggcaaaattt caggtgcttc gtggtggtcg gttataatga catcaacctt
     9061 aaaacttttt aaaaactgaa cttgctcaac atcactgact gcgcagtcaa ccgttatgat
     9121 aagttttggt ttttttgttg ccagtagctt tacaagtgct tttgaattaa gcccgtgacc
     9181 ttctaaatct ctatcgggaa taaaataatc gacttttgcg cctaaatgcc tgaacgttct
     9241 caacaaaagc gaagtcgaag taactccgtc agcatcaaaa tcgccgtaaa tcaagatgtt
     9301 ttcctgattg tcaaccgctt ttgaaatcct gtcaacagca ttttgcatgt cacaaaaaac
     9361 attagggtgg gttaatttta actcaagcgg atttaaaaat tcttttattt cgacttgtgt
     9421 ttttaccccg cgcgaaaaca aaagtctttc cagtaaaggc ttgtttttta attttgaatc
     9481 tttatttact atccactctt ttttcataaa aatatccccg aatttatttt aacaggaaca
     9541 agaataattt aacagtaatt attcaataac agggcggaat tttttaagaa aaattccgcc
     9601 ctgttactat ttaccattta ctcacttaca gcagcttgat ttattaatca aatcaactaa
     9661 tacctcgttt gcttttttaa gctgtttatc atctttgttt tttatgtcat catcggataa
     9721 ttcaaccaaa atatcagggt cgattccttt tttatttatg tctgtaccgt tgggcgtaag
     9781 atatttttgg attgtaatat taattccgga attattcggc agtctgttga tttcttgaac
     9841 aagccctttt ccgaaagatt tttcacccac gattacggct cttttgttgt ctttaagcgc
     9901 accgcttaaa atttcgcttg cacttgcaga gcctttgtta ataagcacca caacgggttt
     9961 tttagtaacg cagccgccca ttgttctttg agtttctttg tagccgtctc tgtcaaccgt
    10021 gcttactata ataccgttag gaagcaaaag gtttgagata aaaatagcgt tactcaatag
    10081 ccctccggga tttgaacgca aatctataat aaacccgtct ttgtcttttg actgaacaag
    10141 tattgtttca aattcttttg aagtattttt acttataaaa gaactcaacc ttatatatcc
    10201 gacgttttta ggtaaaataa cgtcattagg aatttttgta gaaacggatt ttatttcaat
    10261 ttcttttctg gcgattgtat aaggttttat atcttctttt cttttaacca aaagcgtaac
    10321 ggttgttcct tcttcgcctc ttattttatc ggctgctttg tctattgcca ttccttttgt
    10381 actcaccccg tctatttcta aaatttcatc ttcggctaaa agccctgctt tttcggcagg
    10441 tgaagattca atcggagcaa ttaccattaa tttcccgtca cggactgcta tttgaatacc
    10501 tattcctttt aatgagcctt ttattgaact tgtttcttcg gcaaattctt taggatcaag
    10561 aaattttgta taagggtcgt cgagacttgc taccattgta ttaatagcaa catacgcatc
    10621 ttcatctgtt tttatgtaat tgtcgtattt gtgccgccaa gtattccaat cctgcatatt
    10681 gtttgtctgg tcaacgtatt ttgtatttat aagcttccaa acattgtcat acaactctgc
    10741 cgattgataa gaaaacgcac ttagtccaaa tgcaaaaatc ccaaaaaata ctaacccaaa
    10801 cgccgcaaaa cgtttcttaa atccattttt aatatccatt aaatttctcc tcaagtttct
    10861 cttcttatta aatattatta cataataata ttacgatgta aaaaaatcaa aaatgtttca
    10921 ataatttcag aaaattaata aataattgga aaaaaataat tttgagttat aataatgttg
    10981 ttaaattcag caaaaacagg agaaaatcga tgcacgaata cgtatttaag atgaaaaaag
    11041 gtgaaattga aatagaatta aagtctgacg atattgaatt tgttgaacaa cagcttaaca
    11101 aatggcgtga agacatcatg aaaaacaatt caaaatagtt tttatttcga ggcaggttaa
    11161 gtgaaatatt cttttacatt caaaaaaggt gatatttttg ttgaattcac aacaagtgat
    11221 aaacttgtcg tggaaaaaca gtttaaattt tgggttgatg cggcatctga ttacagccgc
    11281 aaacatccat cagcaaaacc tgcaaaagaa aatccaaagg cacctttaaa agaagaaaaa
    11341 gtatcaactg caaagcctca aaaacaggtt gaaacaaaat ccgttaaaaa taaaaaagaa
    11401 gaagcacaag aagagcctaa agaagttttt gacaaagcct ccacactttt aaaaacaatt
    11461 aatttaattc aaaatccgaa actgcccaaa gaagaaaagc caaagtccga acctgatttt
    11521 gaaaaagtgc ttgaaaaaac aattgaaaaa ccgtcttttg aaccttcaaa aaaacatgat
    11581 aaaacttttt tggaacttgt taattctaaa gaatctcagg ataaacttca tcactttata
    11641 acggcggctt attatttatc cgaatacgaa aaaactgata gattcaccct aaaacaagtt
    11701 aattcaaaac ttatgcaaaa tttatccgaa gttattgacc attcggtttt acaggattcc
    11761 atcagacaag aattgattga agttgtgccg gattatacag gaacttctga agtttgcgaa
    11821 tacacgctca caccaaaagg tgaagaattt tttacaaact taaagtaatg tgtcattaag
    11881 taatattaat atttttttct ttgtattttt ttgggctata attaaaacag ccgtttgatt
    11941 agggagataa ggataaatga agaaaaaaat tattataatt tcagcgatta ttgcagcagc
    12001 agtagtggcg aggtttgtat tttcacttta cggacaaatt atgatgggca aaatgatggc
    12061 aaaaacagcc accccgagtg ttgttttagg tgaaatccaa accgcagatg ttttaaaaca
    12121 agttgaagct ccgggcagaa ttttatccaa atacagaatt gacgttcttg caagaattga
    12181 cggctatctg acaaaaagct attttaaaga aggtgaccac gtcaaaaaag gtcaggtttt
    12241 atttgaaatt gaacccgaag aatggtatct tgcggtcaaa aaagcccgcg ccaatgtcgc
    12301 aaacacaaaa gcccagctta tctatgccga aaaacagctt aaaagaggcg aagaacttgt
    12361 aaaacaagat tacatagcaa aagccgctta tgacaacctt ttatcccaaa gagatgcgct
    12421 cagtgcccaa cttaccctaa atcaggcaac cctaagcgat gccctcagaa attacggcta
    12481 tacaaaggtc aaagctccgg ttgacgggca ggtcggtatg attacggtca cagtgggcaa
    12541 ctacgtaaac ccaagtgcag gtgcgcttac caccatttac agcaacaatc cgatttatgt
    12601 tactttccct attgactcaa aagagtatga tattcttaca aaaattgatg ccgaaaatgt
    12661 tgttcgcaaa gtagatttat tttttccgac aggtgaaaaa tatgaaattc agggcaatca
    12721 agactttcat gacaacaaag tcgatgaaac tacaggaacg gtcactatgc gcgccacttt
    12781 tgaaaaccct aaatcaagac tgcttaacgg tgattttgta aaagttattg tgttttcaaa
    12841 aaacacaata aacgtaccga ttgttccgca aacggcagtt ttggaaaacc ctcagggaaa
    12901 atacgtttat gcggttgacg aaaaaaacat tccgcatatt gttcaaatta aaatttcagg
    12961 acaatacaaa gacaactgga ttgtcacaga agggctcaag aaaagcgata taattgtcgt
    13021 tgacggaatt caaaaagtca ttccggacag acccgttaag attgttgata aagagactat
    13081 ggaaaaaatt aaaaaacatc aaaaaccgga agaaggcaaa aaataaacaa tggatacaaa
    13141 tttatttata agacgaccta aatttgcaat agtaatttcg ctggttatag ttcttgcggg
    13201 gcttatttgt atgaacactt tgccgttgga agaatatccg atgataactc ctccgcaagt
    13261 tgttgtaagc gcatcttatc ctggagcaag ttctgatgtt gttgaagcta ctgttgcagc
    13321 accgcttgaa gcccaaatta acggtgttga aaacatgatt tatatgacgt cttcaagtca
    13381 aaacgatggt tattctttaa atgttttctt taaaatcggc acagaccctg atatggcggt
    13441 tataaatatt caaaacaggc ttcagctcgt aactcctcgc ctgcccgagg acgtaagaag
    13501 atacggtctt atggtcaaga aaacggtcgg cggacctggt ttattgatga taagcgtaaa
    13561 ttctccaaac aacacttata accctttgtt tattgcaaat tatgcctcaa tttatgtaaa
    13621 agatgaaatt gcacgtattc cgggtgtaag tgatgtaaat gttttcggct ctgccgatta
    13681 ttcaatgaga atttggttaa atgcaacaaa aatggcagca ttggggattt ctacgtctga
    13741 tgtttattcg gcaattcaac agcaaaacac tcaagttccc gcaggtgata taggtattga
    13801 gccgcttgcc gaaaaacaaa tggttaagtt gactcttaga actcacggac gacttaaaaa
    13861 tgccgatgag tttgaaaata taattgttcg ctcacgtccc gatggttctc aggtaaaagt
    13921 caaagacatt gcaaaagttg aacttggcgc agaaagttat tcattctttt caaggattca
    13981 gggaagaaat accgccatga ttatggtaaa acaacttcct gaagcaaatt cgattgcgct
    14041 tgcaaaaaca atcagaaaac gtttggcaca gttgagcaaa actttcccgc cggggcttga
    14101 atatcacgtt gacagagatg aaaccgaatt tgtacaagag tcgcttaacg aagttgtgca
    14161 gtcaatgttt ttggcaatag gactggtgct tattgtaacc tatgtatttt taagcagtat
    14221 gcgagcggcg atgattccct tctttgcgat tcccgtttct ttgattggaa cttttatatt
    14281 tatagatttg ttcggatttt cgataaattt attaactctt tttgggatgg ttcttgcagt
    14341 cgggcttgtt gtagatgatg cgattgtcgt gcttgaaaac gttcaaaggc acattcaaga
    14401 aggagttaat cctaaagagg caactgaaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn gaagtcagaa ataagtattt gacaagtgca aaatacttcg ttgacaatac
    14581 aaaaaatact atgattctat ttggaatatt tattttaata acggctttat tgtttaaaat
    14641 tatcccgaca agctttttgc ccgaagaaga taaaggtgca atatttactc aaattcagct
    14701 gccggacgga tcttctgctt caagaacaga tattataaca agagaagtcg aaaaaaggat
    14761 tcaaacttat aaaggcgtga aagaaaccat ttctttagta ggatttgccg gtgaaaatac
    14821 ggccttagtt gttacaattt tagataattg gtctaaacgc catgggtcaa aactctctgc
    14881 aaacggaata atcggcaaga taagacagga tttcggaaat tatccttcag ctgttattgt
    14941 gtcgttttcg ccgccttcaa ttccgggctt gggcatgttc ggaggttttg aatatcagct
    15001 cttggataaa ggagacagaa gccctcagga tttatactta gaatcaatga aatttgtcgc
    15061 cgacgcaaac aaaaatcctg ccctttcaag tgtttacact caatatacgg caaatttacc
    15121 gcaattaatg tttgatgttg acacggcaaa ggcaatggct caaggagtga ctattcagga
    15181 aatttacact acgcttgccg caaatttcgg acaaacttac gtaaatgatt ttaataaata
    15241 cggtcgtgtt ttcagggtta tgatgatggc tcaggatgaa ttcagagcta aacgacaaga
    15301 cattgataaa atatttgtca aaaatattaa cggaaaaatg gttcctctaa cctccgttat
    15361 caaacttaaa gatgttgtcg gaccctatac tataacaagg tttaacatgt acaaggccgt
    15421 tacaatgaac ggaagtgcag caaagggagt aagctcaggt aaagcaatga aaacaatgga
    15481 agatttatca aataaaattt tacccaaaga tatgggatac gcctggtcgg gaatttcttt
    15541 acaggaacag gagtcaagcg ggcaaatcgg atttatactt gccacagctt tgatatttgt
    15601 atatttattc cttgtagcac tttatgaaag ctggactttg ccgcttgccg ttatgttgat
    15661 ttcaccgatt gctattatgg gcgggctttt gtttcaatat gtattcgggt attcgcttga
    15721 catatattct caaatcgggc ttgttatgtt aatcggatta agtacaaaac aggctatttt
    15781 gattattgaa tttgcaaaag aggctaaaga aaacttaaaa atgcactata atgatgcagc
    15841 aatggaagct gcaaaattaa ggtttagagc ggttatgatg acagctattg catttatttt
    15901 gggaatgctg ccgttggtct tcgcatccgg tgccggctcg acaagccgcc gctcaatagg
    15961 tatgactgta ttcggcggaa tgattgcggt tgcctttatc ggcacaatgc ttgttcctgc
    16021 gttttttgtc gcagttgaaa aaatgaaaga taatgttgag gaatttattt taaagagaaa
    16081 acaacaatga aatcaattaa aaaattaatt ttaatattaa taatatttac ttttagtatt
    16141 ttacctactt tagcttttgg actaaaggct acaaaaagtg aaaaaatatt ggacgaaaca
    16201 cttggtaatt ctattgtaaa agaagaatat cccgcagagg ctcaggtttc tcccgtcgag
    16261 acagatgccg ataagcagga aaaaaatctt gtaatcgagg gctctgtcca aaaaacaatc
    16321 gacataactt tgggtgaatg tataaaactt gccttgggaa acaatccgag aattaaggca
    16381 gcactcaacg aagctcttgc ctcgcatgcc agaatttcac aagcctggtc aaattatttc
    16441 cctgaacttg attggcaatc cggttacaca aaaacaagaa atcttcaaac ggcgactatg
    16501 attggcaata caagtgcttt taattactac cttttaggtc aggtaagcgt ttctcaaatg
    16561 ttatatgatt ttggagttac ccaaaacact gttaccatta aaaaattgga ttatgaagct
    16621 tacaaaacct ctttgacggc ggttgtaaac gatgttattt gtcagacaaa agatgcttac
    16681 tacaatcttc tttttgctta cgaaaacaga aatgtagcac aagacaccgt tcaaaaatat
    16741 gaaatgtttt acaatcaggc aaaagctttt tatgaaatag gaatgaaccc taaagttgat
    16801 gttactattg cagaagtcaa cttaagcaat gcaaaattaa atttaattca ggcggaaaat
    16861 tcaatagatt tggcagttgc cactttaaac aatgtaatgg gtgttcctta tatcaataaa
    16921 tacaatattc aggagcgttt aaaatttaac cctattacta tgagtatgaa taaagcattt
    16981 gaacttgcaa aagaatcccg ccccgaattg aaattagcag aaattaaagt tcaggaagca
    17041 aatcaggctg taaaattagc taaaaaagca tattttccga cacttgaagc ttcaggttct
    17101 tacgcaagag gcggcgaaca aattaatgaa acttacggct acaattacgg ggtttattta
    17161 aattttccga cagtaaacgg aatgttaatt aaaaatcaga ttaaagaagc acgttcttta
    17221 tacgacaggg aaattgcaaa tgcacaaatt accaaaaaca atatttattt ggaaattcaa
    17281 caggcatatt taatgcttta tgaaaagaaa aatcaactgc cagttgcttt tttgcaggtt
    17341 aaacaggcaa aagagaatta tgatctatcc ttcgggcgat ataaagtcgg agtgggaaat
    17401 ccaaccgaac ttaaagatgc tcaaaattct tatcagtcgg cacagttaaa ttattacaaa
    17461 gccctttatg aatataattc cgccaaagcc aagcttgaaa aagctatagg taaaaatatt
    17521 atagccgatg acgatgaaag tattgattta gagacataaa caaggaaaga aaaatgttaa
    17581 gcacaaacca atatattaaa ccgttaacca cttgcataaa tgaaaacggc aacctttcaa
    17641 taggcggctg tgatttagta gatttagcac aaaaatacga aacaccgctt tatgtgatag
    17701 atgaaaaaac gctgcgaaca atctgcaagg aatacaagca ggcattttct tcctatgagc
    17761 gtgttaaaat gctctacgcg tcaaaggctc ttatgacaaa agcagtcgca aaaattgtaa
    17821 attcagaagg cttcggcttt gacgctgttt caggcgggga actttataca attaacaaaa
    17881 caggaatcga tatgtcctgt gtacttttta acgggaacaa caagtcttac gatgaacttg
    17941 atatggcact tgaacttgga gtgggaagaa tctctgttga caatttttta gagctttctt
    18001 tgttaaatga gattgccaaa aaacacaata aaaaagtcga tattttactc aggattactc
    18061 cgggaataga atgtcacacg cacgaataca ttcaaacagg aaatcttgat tcaaaattcg
    18121 gattcgacct gtcgcaaatc catgatgcaa tagagcttgt acttgaacag tatcccaata
    18181 tcaatttaaa aggttttcac acgcatgtag gctctcaaat ttttgaaaca caaatttttg
    18241 aagatgaaat tgatattttg ataaaagaaa ttgtatatgt gaaagaaaaa ttcaatattt
    18301 tactcgacga aattaatatc ggcggcggct tgggggtaaa atatatccca actgatcttc
    18361 cgccatcggt ttttgaaatc ggagagttag taataaatgc cttaaataaa agtattaaaa
    18421 aacacaatat tcctgctccg ctgctctatc ttgagccggg cagaagcata atttcaacag
    18481 cgggtgtaac cttatatacg gtaggaagtt caaaacaagt tccaaacggc actctgtatt
    18541 ggtctgtaga tggcggaatg gctgataatc cacgcccaag catgtatcaa gcggaatata
    18601 cggcgcaaat agccaacaaa cctgagcaag attgttcaca aaaagttaca atcgcgggga
    18661 aattttgtga atcgggagat attttgatta aagacataaa tttacccgag cttgaagaag
    18721 gcgatatact gtgcgttttc aacacagggg catacaatta ttcaatggcg agcaactata
    18781 accgctttca aaaacccgca gcggcacttg taaataattc acaatctgat atgataataa
    18841 taagggaaag tttggatgac ctgctttctc atgatgtctt acctgccaga ttggataaat
    18901 gatgatttta gattctcttt ttacatcttt acactctcaa ttgaacgcaa ttaagtggtc
    18961 tttaaactgg cttgatttag ttgaagtttt tgctattttt ataatgctct ttgcttttta
    19021 cagaagattt atcaaaaata cccaatcaga aaaacttgtt aaaggaatgg tttttttact
    19081 tattgcctgg gttacaagtg aaattttgat aagtctgggg cttcaaattc ttggtgtttt
    19141 cataaaatca atggtaacat tggtagcctt aagtttaatt gttgtattcc aacctgaatt
    19201 gagaagattt ttagggtatt tgggtcaacc cggttttatc agcagaggct tttggaaaaa
    19261 ttcaaacaac aaagaaagca taaacgttgt aaaagaagtt attgaagctg tcaaatatct
    19321 ttcaaaatca agaataggcg cgttgattgt ttttcaaaaa gaaatgaacg cttctactta
    19381 ttttgatgtg ggaacaaagt taaatgccga tgttacaaca gagttgcttt tgacaatttt
    19441 tcaccctaac accccgctgc atgatggtgc tgtcgtgatt aaaaatgata aaatactttc
    19501 agccggcgtt ttgcttcctt taaccgaaga ccctaaatta agctggaaat acggcacaag
    19561 gcacagagcg gcaattggaa cttcagaaac aagtgattcc gggtgtttgg tggtttctga
    19621 agaaacaggt gatgtttcaa tgtctgttga cggagttttg aaaaaatacg atgacataac
    19681 aactctaaaa accgatttgg aaaccttctt gggatacgct gattacgatg aaatccacaa
    19741 gaaaacaatt tttaaaataa ataatttgat taatataaaa aaggctgaaa taatgcctaa
    19801 agtaaacaag gataaggttt agcaagtcaa aatgttggtt aagaaaacaa ataaagaaag
    19861 ttcttttata tcctggatag taaaactaat tcttttaaca acaggagcct ttattgcagg
    19921 atttggttta gaaggatttt taattccaaa cagtatgatt gacggcggaa ttgtcggaat
    19981 ctcaattatg ctaagctatt taaccaaaat aaatcttggt attttactgt tttgcttaaa
    20041 tcttcccttc atatttcttg cgcttaaaaa actgggacgt atttttgtcg tccaaacagc
    20101 ttacgctata acaatgcttt ctttgagtgt taacgtcttt cataaaatgc atgtgaccga
    20161 tgatcttttg cttgctactg tttttggcgg agttgttttg ggacttggcg ttgggcttat
    20221 tttaagaagc aatgccgctt tagacggaac agaaatcttg gcaataaaac ttgccaaaaa
    20281 agcaggattt tctatcggcg aaataattat gttctttaac ttttttatct ataccgccgc
    20341 aggatttctg ttcggttggg acagggctat gtattcaatt ttagtttatt ttatagccta
    20401 taaagttatt gatattgttt tggaaggtct taacgaatca aaatccgtaa ctataatttc
    20461 taaccgcgca gaaaaaatcg gcgaagaaat catcaaacga cttgacgtgg gcgtaactta
    20521 tttgagcgga aaaggcggct atacaggcga agataaaaaa ataatctact gcgttatctc
    20581 aagacttgaa cttgtaaaac ttaaagagtt agtaaattta atcgataagc agtcttttat
    20641 cgccatagaa aatgttcacg aagtagaagg tgtcagaatt aaacagaaac gtgcaggtaa
    20701 aaaaaaataa aacttaacac atagacaatt tttttaaaat gtattatatt aagaaaagag
    20761 atttaaaaga taagggtggg caatggcaaa acatatagat acaattccta tagaagtaag
    20821 tattttaaca gttgatcatg atattaaagg cactgtgcat gtttcaaaat acacaagcac
    20881 taaccgtgaa ttgacagatt tgcttaacga ccgcgaaaga agatttcttg ctgtcactaa
    20941 tgccgaaatt tattccagaa attcaaccca aacacccaga agatataatt ttttagaaat
    21001 ccacatggat tatgttttaa tggttcatcc atcaactcaa gcagtcttca aagaatcggg
    21061 taaggctcag gaagacattg caagattcag agaattaaga cagaaactta acca
//
LOCUS       DAOQAU010000030        20030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2798_length_20030_cov_5.810363, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000030 DAOQAU010000000
VERSION     DAOQAU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_20030_cov_5.810363"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="PKI94_05545"
     CDS             <1..1758
                     /locus_tag="PKI94_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNW26241.1"
                     /translation="RRWSDGLHQAIEAKENVKIQDETQTLASITFQNLFRLYPKLSGM
                     TGTAMTEEAEFGKIYNLEVTSIPTNKIDIRKDFPDVIYKTEKQKYLSVVDDIIEMNKI
                     GRPVLVGTISIEKSEYISDLLKKRGINHNVLNAKHHEKEAYIIAQAGRFGAVTIATNM
                     AGRGTDILLGGNAEYLAKEELEMQGITSESSNYEQKKNEILQKSRETTEEEHKKVVQV
                     GGLHVIGTERHESRRIDNQLRGRAGRQGDPGSTRFFLSLEDNLMRIFGGDKIVGLMEM
                     LNVEETMAIESPIISRQIQSAQKKVETYHFDIRKSVLEYDDVMNVQREKFYAQRRRVL
                     RGDKLNEHIFYMIEQEIDRLIKSYISPEMQPEEYIYEDLQTMIKELHSTIPQMSTFSV
                     ADIQGLKFDAIYEKIKENALLAYQNHEREIVDFYNDVIAKYEESPKVEEPFTVKNVMR
                     NIEKDILLRVIDSKWIDHLHNIDMLRDGIGLRAYGQKDPLIEYKKEAYDLFNKMMFEI
                     QGETARHIFRTKFGVQIVNMKDDEVIETDLARAAEQFHPSEEVEQAIRQPEKHDKKVG
                     RNDPCPCGSGKKYKHCCGG"
     gene            1877..2974
                     /gene="ychF"
                     /locus_tag="PKI94_05550"
     CDS             1877..2974
                     /gene="ychF"
                     /locus_tag="PKI94_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNW26242.1"
                     /translation="MLRAGIVGLPNVGKSTLFNALTSAQNAQSANYPFCTIEPNIGVV
                     NVPDERLYVLKPLVKTEVVIPTAIEFVDIAGLVKGASKGEGLGNQFLHTIREVDAIIQ
                     VVRCFDDENTIHVAGKVNPVDDIEIINMELALADLSTVEKRQARISKVAKSGDKDALL
                     EEKVLKKLLELLSEGKFINVKDHFEGDELFVIRGLHLMSTKPVIYCANVTEFDLKNGN
                     LYTKQVEDYAKTHNAEVVIVSAKIESELSELDAEEAKEYLQELGIEDSGINRMIKAVY
                     HLLDLRTFITAGEMEVRAWTIPAGAKAPQAAGVIHTDFEKGFIRAEVTAYKDFIACGS
                     YAACKDKGLTRLEGKDYIVQDGDIVHFRFAV"
     gene            complement(3128..4465)
                     /gene="secY"
                     /locus_tag="PKI94_05555"
     CDS             complement(3128..4465)
                     /gene="secY"
                     /locus_tag="PKI94_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNW26243.1"
                     /translation="MQQKVQMPTTADLMSMWHASGLKEKIIFTFIMIAAFRFGVQMPI
                     FGINNEIFSHMASQNNIIGFLDLFSGGALGKVSIFALGIGPYITSSIIIQLVSVVIPS
                     LEKLQKEEGEAGRRKLAQYTRVFTVILAIFQSLIFMVYMSKLPGAILPGVNFGLFFIS
                     SIAVLTAGSVLVMWLSELITEKGIGNGGSLIIFIGILSGIPIYAQRTGQLVARDSALQ
                     LGLVLLLAIFFATMVFIVIMQEAQRKVVIVNPKRQVGNKVYGGVNTFIPFKLNPGGVM
                     PIIFAIAILLFPTTVLSVVGQANIHNLALKNFIMQLTQIMNPNGATYYILYFVLIVAL
                     TFFYASIMPNMQPKEIANNLKKYGSSIPGIKPGKPTAEALDKILSKTTFIGAMGLGII
                     ALVPSFTSYITNIKTLQGIGATSLIIMVGVALDLINQVRTHLLARNYESFLKE"
     gene            complement(4465..4905)
                     /gene="rplO"
                     /locus_tag="PKI94_05560"
     CDS             complement(4465..4905)
                     /gene="rplO"
                     /locus_tag="PKI94_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNW26244.1"
                     /translation="MNITDLKPAEGATKKSKRVGRGRSSGHGKTSCRGHNGEGQRSGR
                     SAKRGFEGGQMPAFRRLPKLKGFKLINQLNYAEINVGMIEKLGLKEVSLEILKENKKA
                     HPSTVGLRVLGNGEISKSVKIKASYVTPQAKTKIEAAGGKVEIV"
     gene            complement(4990..5352)
                     /gene="rpmD"
                     /locus_tag="PKI94_05565"
     CDS             complement(4990..5352)
                     /gene="rpmD"
                     /locus_tag="PKI94_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNW26245.1"
                     /translation="MAETKNKTKATAKPKAKTTATKPKTAAKPKAKPAAKIEEKIAVK
                     PAVKAEVKTKAKAEIKAKTNQIKIQLIKSLIGSSQAQRKVAKALGLTKTNQIVEQAET
                     PIIMGMVRKITHLVKVVK"
     gene            complement(5368..5877)
                     /gene="rpsE"
                     /locus_tag="PKI94_05570"
     CDS             complement(5368..5877)
                     /gene="rpsE"
                     /locus_tag="PKI94_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNW26246.1"
                     /translation="MDTQDAGAPVQSEWKERVVQIRRVTKVVKGGKKLSFRAVVIVGN
                     QKGQVGVGCAKAAEVIIAIQKAIADGRKNLIEVPIFKTSIPHPITGCAGAGSVMLRPA
                     SQGTGIIAGGAVRSVLELAGVENILCKSLGSKSPLNAANATLDALRNLRPFKEVAKRR
                     GKTVAEILS"
     gene            complement(6405..6477)
                     /locus_tag="PKI94_05575"
     tRNA            complement(6405..6477)
                     /locus_tag="PKI94_05575"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6441..6443),aa:Glu,seq:ttc)
     gene            6778..9258
                     /locus_tag="PKI94_05580"
     CDS             6778..9258
                     /locus_tag="PKI94_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNW26247.1"
                     /translation="MFERFTEKAIKVIMLAQEEARRLGHNFVGTEQVLLGLIGEGTGV
                     AAKTLKSMGVNLKDARVEVEKIIGRGSGFVAVEIPFTPRAKRVLELSWDEARQLGHNY
                     IGTEHLLLGLIREGEGVAARVLENLGVDLNKIRSNVVKMLGESKPQAVSSGASSSSGS
                     KAKTPSLDEFGTDLTLAAQELRLDPVVGREKEIERVIQILARRTKNNPVLIGEPGVGK
                     TAVAQGLAIRIVNSEVPDILDGKKVIQLDMGLLVAGTKYRGEFEERLKKIMDEIRQAG
                     NVILVIDEMHTLIGAGAAEGAIDAANILKPALSRGEIQVIGATTLDEYRKHVEKDSAL
                     ERRFQPIIIEEPTVEETIEIIKGLKAKYEDHHKLNISDESIVAAAKLSSKYITDRFLP
                     DKAIDVLDEASSKVRLQMSSLSPEGKELDKELRALIKEKEDAIRNQEFEKASQLRDDE
                     ADLKERIREMSAKWREEHEANKPTVTEEQIAEVIATMTGVPVTRLTEGESDRLLKLED
                     TLHERVIGQSDAVVAISKAIRRARVGLKSPNRPIGSFIFSGPTGVGKTELAKALAEAM
                     FGSEDNMIRVDMSEFMEKHSTAKLIGSPPGYVGYDDGGHLSEIVRKKPYSVILFDEIE
                     KAHPDVFNIMLQILDDGRLTDSKGRHVNFKNTVIIMTSNVGASMIANQSKLGFTSATD
                     EKKDKYEKLKDTVMEEMKKAFRPEFLNRIDDIIVFSHLSKDEIRQIVDLMLKDLFKRL
                     EERELSIEVTDEVKDFLAKDGYSEAYGARPLRRLIQKRIEDILAEEILTNKYSAGDVI
                     VLKMQDDKITFESKGKSSKDNKKEEAVK"
     gene            9525..9692
                     /gene="rpmG"
                     /locus_tag="PKI94_05585"
     CDS             9525..9692
                     /gene="rpmG"
                     /locus_tag="PKI94_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNW26248.1"
                     /translation="MGIKGRVDKMVVLACEDCKERNYTTVKNKKTTKERMELKKYCPS
                     CQKHTAHKETK"
     gene            9761..9833
                     /locus_tag="PKI94_05590"
     tRNA            9761..9833
                     /locus_tag="PKI94_05590"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9794..9796,aa:Trp,seq:cca)
     gene            9978..10175
                     /gene="secE"
                     /locus_tag="PKI94_05595"
     CDS             9978..10175
                     /gene="secE"
                     /locus_tag="PKI94_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNW26249.1"
                     /translation="MNETINVITAYLKGVRAEWGKITWPERRQVFAETFFVVIIVFVF
                     TVAVYFMDVIFKWVLGFIPHR"
     gene            10197..10829
                     /gene="nusG"
                     /locus_tag="PKI94_05600"
     CDS             10197..10829
                     /gene="nusG"
                     /locus_tag="PKI94_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNW26250.1"
                     /translation="MVKKEKKELTEDLLTEAVLEEKREDAAPVHEKLDRKGQKRWYIV
                     HTYSGHENKVKVNLEKRIEYMNMGEKIFRVEVPQKTVTQVKAGKKQEKEEKIFPGYVL
                     VEMIMDDDSWYVVRHTAGVTKFVGSAKKPIAAKDSEIKKIIHRTQAQTQKVQLDIKAG
                     DKVRIVSGPFAEFIGDITEVYPDKSKLRANVSIFGRETPVELEYKQIQKL"
     gene            10876..11301
                     /gene="rplK"
                     /locus_tag="PKI94_05605"
     CDS             10876..11301
                     /gene="rplK"
                     /locus_tag="PKI94_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNW26251.1"
                     /translation="MAKKVIGKIKLQIEAGKANPSPPVGPALGQHGVNIMDFCKQFNA
                     KTEAQAGYIIPVVIDVYEDRSFSFITKSPPAAVLIKKALNLPKGSAVPNKDKVGQITQ
                     AQMEEIAKTKMEDLNATSLESAVKMIKGSARAMGVTVAE"
     gene            11355..12080
                     /gene="rplA"
                     /locus_tag="PKI94_05610"
     CDS             11355..12080
                     /gene="rplA"
                     /locus_tag="PKI94_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNW26252.1"
                     /translation="MTKLTKRQKKIQEMLEGFEQPATMADAIKKIQEISKETSKFNET
                     VECHMRLGIDVKHADQQIRSTTVLPAGLGKDIRVCVIAKGAKATEAKAAGADEAGAEE
                     VIDKIDKGWFEFDTLIATPDCMGMLGKLGRVLGPKGLMPNPKTGTVTLDVATAVKDAK
                     AGKVEFRADKQGMIHCPVGKIQFKTEDLLKNFATLAEAVIKAKPSSSKGTYLKSVYLA
                     TTMGPGIKLDSKNLASEIKEYVS"
     gene            12335..12949
                     /gene="tmk"
                     /locus_tag="PKI94_05615"
     CDS             12335..12949
                     /gene="tmk"
                     /locus_tag="PKI94_05615"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNW26253.1"
                     /translation="MEKGLFITFEGADGCGKTTQMNLLHEYLILKGYDVVLTREPGGK
                     GLGERIREILLNYEGEVSSQAEAFLFLADRAQNIDVIVKPAVDAGKIVLCDRHIDSTI
                     AYQGYGRGVNLERLKMLNDIATNKFMPDLTYVFDIDVETSQSRVGKNKDRMESAGVEF
                     FEKVKKGYLELAKKEPERVKVINSANTIENIFAQVKEIFEKTIC"
     gene            complement(12939..>14048)
                     /locus_tag="PKI94_05620"
     CDS             complement(12939..>14048)
                     /locus_tag="PKI94_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNW26254.1"
                     /translation="LVQIPSPSMKEEKVIEWIKNFCADNGINCKSDDYGNLIITVSGT
                     VTDKQPLLLSAHMDVVGDASPINLQINDDFIETDKTRTLGADDKAGVACALMLAYEFS
                     HIYPLEIVLTRDEEHGMSGIKHLDFSKLNSKYALVLDADKLGQLLVSGASYTNAQLNL
                     KTSYGGHSGLDIGDEKRLNAAKLIAELVNEIPQGVFYKDETGTITSINIGTIIAGNTQ
                     NGTPVTNVINTKAQATYSIRSASFEKENELKAIIQNIVEKFNKKYDSLANAEIIFSQH
                     LPPFEKAQDDFIEKIHTKVCAMLGINQDISSFHAGAETHIYAQNKNSKGETICPFLVG
                     LADVFNMHSIDEKIDYKTLIQGYNLLKEMFLEFNK"
     assembly_gap    14049..14148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14247..14438)
                     /locus_tag="PKI94_05625"
     CDS             complement(14247..14438)
                     /locus_tag="PKI94_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26255.1"
                     /translation="MSGFEKNEKKPYLGIMFKCCNVYTRIYKNKTGDAYVGRCPKCSR
                     SVRIPIGEGGTNQRIFYAD"
     gene            complement(14466..16304)
                     /locus_tag="PKI94_05630"
     CDS             complement(14466..16304)
                     /locus_tag="PKI94_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNW26256.1"
                     /translation="MNRVLWKLFILFALLMQFVCPAFADDFNFFSDDSEIIFSQKVYP
                     LNAIDPTIVSNTVGSFYPGLRGANQMIVYTPAFGFRTNTNEFGSEAIVCKNITTALGG
                     ADSLIPRDGFVISGHGSAKKWINENVILGSKVYVNPTDKTLTVYITSDSFLYGAQQKI
                     KEANEIVNYYKERSLYYDAKKPKMYIEQAQDYLEKAKHDPSNVQKYSSQAIESANLAL
                     ANALPYKADELKGVWIRPTETSSEEITKTLDRLKASGINNIFLETYFHGKTIFPSKTM
                     SDYNFIRQNEKFAGFDPLKIWIQEAHKRGIKVNIWFETFYVGNEPPNSNSKSILAVKP
                     AWANKTKKDYEKQGPTPSVSEHNGYFLDPANPEVQDFLEYLIKEIITNYSPDGINLDY
                     IRYPQSLAAKYPGYDLSNWGYTEYARADFKSQYCKDPVEILPDDELWQVWDKYRQDNI
                     TEFVERISKLTHNKNILLTAVIFPDRQKALETKQQDWKTWSQRGYIDGFTPLLLTCDA
                     KTANIMMQDINRNKCDCTKLYSGIFVTFMGGSNEDLIRQIHEARKLESKGIILFDYAH
                     LDDKYKNTLMASVFLPTEDDTYKIQPNTRYQFQRKKRTPFKLRKII"
     gene            16495..16848
                     /gene="ndhC"
                     /locus_tag="PKI94_05635"
     CDS             16495..16848
                     /gene="ndhC"
                     /locus_tag="PKI94_05635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNW26257.1"
                     /translation="MKELIFLAVFVVLSGLFALAALIASYICSPRTLFEDKKTHYECG
                     MELFSDARIQFDVKFFNFAIMFLIFDVETIFLFPFAVNFNQLEAFALIEVTIFVFLLI
                     FALWFAIKKDILRWQ"
     gene            16809..17279
                     /locus_tag="PKI94_05640"
                     /pseudo
     CDS             16809..17279
                     /locus_tag="PKI94_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            17413..17871
                     /locus_tag="PKI94_05645"
     CDS             17413..17871
                     /locus_tag="PKI94_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNW26258.1"
                     /translation="MNFEALQNFDIQVIKQENSFDKIRVDKSNLVDLLSFLKNNAEFD
                     FDTLLSLIAIDLKDSFELIYCVYSTKLNTQRQISVLLDRNLPKVNSIVDVYKSAYFDE
                     CEIYDLFGINFENNPDLKRLFMPKGWIGNPLRKDYIADDSRLAWNEVKNA"
     gene            17864..18976
                     /locus_tag="PKI94_05650"
     CDS             17864..18976
                     /locus_tag="PKI94_05650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNW26259.1"
                     /translation="MPNSERTRMKINIGPQHPSTHGVLRLVLELEGEIIKSCEPVIGY
                     LHRGMEKMAENRTYLQYLPTVDRVDYLSGFFNSYAFCDAVENLAKIEVSKRTKYIRVL
                     TLELNRLASHLLWLGTYMLDLGATTPLFYCFRDREIILHLFEELTGQRLMYNYYTFGG
                     LRHEVSSDWLNKVKDFVKIMPSRINDYEGIITNNPIFVARAKGVGIISKNTALQYSMT
                     GPNLRASGINVDFRKQKPYLVYGDIEFDTAVAQNGDCYDRYNVRIEEMRTCLKIISQC
                     VDWLLVNQGEVLSTVKQHLLKVPEGEYISNVEAPRGLCSCYVKSDGSSNPYRVKWRTG
                     SYYSVQILPKLLIDSMYPDLMTIFGSLDVILPEVDR"
     gene            18976..>20030
                     /gene="nuoH"
                     /locus_tag="PKI94_05655"
     CDS             18976..>20030
                     /gene="nuoH"
                     /locus_tag="PKI94_05655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNW26260.1"
                     /translation="MDYLYFLYLNLLEKFHIPAFVGTVTFPVLPFCIVAVVLILVVLF
                     LVLLERKVLAVFTQRKGPNRVGPWGLFQIIADAIKLLCKENITPEKSDRFLFMIAPLL
                     AFVPVMVVWGLIPFSSEFDFISYSVSGLLFISIASIPIIAILLAGYASNNKYSLIGSI
                     RSVVQAISYELPMVFSFLSIVLLASSMNLKQIVFAQISIYPFCGWFIFPAFLGFVVMF
                     ICALAELNRCPFDLPEAESELVSGYNTEYSGMRFAFFFLAEYAMAFIMCAFLATLFLG
                     GFLSPLGFYISEKIFGYNTLSQILVYFEQVFWIFLKTFALFICILWVRATLPRLKSDQ
                     LNAFAWKFLLPVSILNL"
BASE COUNT         6304 a         3489 c         3978 g         6159 t
ORIGIN      
        1 agacgctggt ctgacggact tcatcaagct atcgaagcaa aagagaacgt taaaattcag
       61 gatgaaaccc aaactcttgc aagcataact ttccagaatt tattcaggct ttatcctaaa
      121 ctctcaggta tgacgggcac tgcaatgacc gaagaagccg aatttggaaa aatttataac
      181 cttgaggtaa cctcaatccc taccaataaa atcgatatca gaaaagattt tcccgatgtg
      241 atttataaga ccgaaaaaca aaaatattta tcggtagtag atgacattat agaaatgaat
      301 aaaatcggaa gacctgtgtt ggtgggtaca ataagtatcg aaaaatccga atacatctcc
      361 gatttgctta aaaaacgcgg aattaatcac aatgtcctga atgccaaaca tcacgaaaaa
      421 gaagcttata taatcgctca ggcggggcgt tttggtgcag ttaccattgc aactaacatg
      481 gcaggcaggg gcacagacat tttgcttggc ggtaatgccg aatatttagc aaaagaagaa
      541 cttgaaatgc agggaattac ttctgaaagc tccaattacg agcaaaagaa aaatgaaatt
      601 ttacaaaaat cgagagaaac caccgaagaa gaacataaaa aagttgttca agtaggcgga
      661 ttgcatgtta taggaacaga aagacacgaa tctcgccgta tagataatca gttaagaggc
      721 cgcgcagggc gtcagggaga ccccggctcg acaaggtttt tcctttcttt ggaagacaat
      781 ttaatgagaa ttttcggtgg agataaaatt gtcggactta tggagatgct taatgtcgaa
      841 gaaaccatgg caatagaatc tcccattatt tcaagacaaa tccaatcggc tcaaaaaaag
      901 gttgaaactt atcactttga tataagaaaa agtgtgcttg aatatgacga tgttatgaat
      961 gttcaaagag aaaaattcta cgctcagcgc agaagagtgc ttcgcggcga taagcttaac
     1021 gaacacattt tttatatgat tgaacaggaa atcgacaggc ttattaaaag ttatatttcg
     1081 cctgaaatgc agccggaaga atacatttat gaagaccttc agaccatgat taaggaactt
     1141 cactctacta ttcctcaaat gtcaactttc agcgtggcgg atattcaagg actaaaattt
     1201 gatgctattt acgaaaaaat caaagaaaat gcacttttgg catatcaaaa ccatgagcgc
     1261 gaaattgttg atttttataa tgatgttata gcaaaatatg aagaaagccc aaaagttgaa
     1321 gagccgttta ccgttaaaaa tgttatgaga aacattgaaa aagatatttt gttaagggtt
     1381 atcgacagca aatggattga ccacctgcac aatatcgata tgctaagaga cggcataggg
     1441 cttcgcgctt acgggcagaa agaccctctt attgagtaca aaaaagaagc ctatgatttg
     1501 tttaataaaa tgatgtttga aattcaaggc gaaaccgcaa gacacatatt cagaacaaaa
     1561 ttcggcgttc agattgttaa catgaaagac gacgaagtta ttgaaacaga tttagcaaga
     1621 gcggcagagc aatttcatcc ctcggaggaa gttgaacagg caattcgtca acccgaaaaa
     1681 catgacaaaa aagtcggcag gaatgaccct tgtccttgtg gcagcggcaa aaaatacaag
     1741 cactgctgcg gagggtaaat gtatgacaaa aacacttgcg tcatcctgaa cttgtttcag
     1801 gatctaacca gaaaactacg caggaaagat gctgaaataa attcagcatg acatttgata
     1861 aaaaaaggaa ttgataatgc ttagagcagg aatagtcgga ttaccgaatg taggaaaatc
     1921 aactttgttt aacgcactga caagtgcgca aaacgcgcag agtgcaaatt atccgttttg
     1981 cacaattgag cctaatatcg gagttgtaaa tgtgcctgat gaaagattgt atgttttaaa
     2041 gccattagta aaaacagagg ttgtaattcc taccgcaatc gaatttgttg atattgcagg
     2101 gttagtaaaa ggcgcaagta aaggcgaagg cttgggaaac caatttttgc acacaataag
     2161 agaagttgat gccataatcc aggttgtacg ctgttttgat gatgaaaata caattcacgt
     2221 cgcaggaaaa gttaaccctg tcgatgatat tgaaattata aatatggaac ttgctttagc
     2281 tgatttgtcc actgtcgaaa aaaggcaggc taggatttca aaagtggcaa aatcaggcga
     2341 caaagacgca cttttagaag aaaaagtttt gaaaaaattg ttggaattgc tttctgaagg
     2401 taaatttatc aacgtcaaag accattttga gggtgatgaa ttgtttgtta tcagaggttt
     2461 gcatctgatg tcaacaaagc cggttattta ctgtgccaac gttacagaat ttgacttgaa
     2521 aaatggaaat ttatatacaa aacaggtcga agattacgca aaaactcata acgctgaagt
     2581 agtaattgtt tctgccaaaa ttgaatctga attgtcagaa ttggatgcag aagaagccaa
     2641 agaatattta caagaattag gtattgaaga ttcgggcata aacaggatga ttaaagccgt
     2701 ttaccatctc ttggatttga gaacttttat aactgcaggc gaaatggaag tcagagcttg
     2761 gacaattccg gcaggtgcta aagcacctca ggctgcagga gttattcata cagattttga
     2821 aaaagggttt atcagggcag aagttaccgc atataaagat tttatagctt gcggttctta
     2881 cgctgcttgt aaagataaag gcttaacaag acttgaaggc aaagattaca tcgttcaaga
     2941 cggcgatata gtgcacttcc gctttgcagt gtagttaaaa ttgtcatccc gcataagcaa
     3001 agcgtattgc gggatctatt tggtgctgtc tatatataga taccgcaaca agtgcggtat
     3061 gacacatatt aattcaagag ggcgggattt tctttcagaa aatcccgccc tcttgatata
     3121 tttaccccta ctctttcaag aagctttcgt aatttcttgc aaggaggtgt gttcttacct
     3181 gattaattaa gtcaagagca actcccacca tgattatcag cgaagttgcg ccgataccct
     3241 gaagggtttt tatattggtg atatagcttg taaaagaagg tacaagtgct ataattccaa
     3301 gccccattgc gccgataaaa gttgttttgc tcaaaatttt atcaagtgct tccgcggttg
     3361 gttttccggg cttaattccg ggaattgacg agccgtattt tttaaggttg tttgcaattt
     3421 cttttggctg catatttggc ataattgaag cgtaaaagaa agtcaatgcg actattaata
     3481 caaaataaag tatataatat gttgctccgt tagggttcat aatttgagta agctgcatta
     3541 taaagttttt cagagccaaa ttatgaatat ttgcctgtcc tacaacactt aaaacagttg
     3601 tggggaaaag cagaatcgca atcgcaaaga taatcggcat aacgccgccg gggttaagtt
     3661 taaagggtat aaaagtatta actccgccgt aaactttgtt tccgacctgt cttttagggt
     3721 taacaataac aacttttctt tgtgcttctt gcataattac aatgaaaacc attgtcgcaa
     3781 agaaaattgc aagcagcaaa acaagtccta attgcaaagc cgaatccctt gcaacaagct
     3841 gtcctgttct ttgggcataa atcgggattc cagacaaaat gcctataaag ataatcaaag
     3901 agccgccgtt tccgatgcct ttttctgtta taagttctga aagccacatt acaagcacag
     3961 aacctgcagt taataccgcg attgagctta taaagaacaa tccgaaatta acaccgggta
     4021 aaattgctcc gggaagtttt gacatgtaaa ccatgaaaat aagcgactgg aatattgcca
     4081 aaatgaccgt aaacactctt gtgtattgtg caagtttcct tcttcccgcc tcaccttctt
     4141 ctttctgaag tttttccaaa gaaggaatta caacagatac aagctgaatt ataattgaag
     4201 aagtgatgta aggccctata ccaagcgcga aaatagaaac tttacccaac gcaccgcctg
     4261 aaaacaaatc taaaaatccg attatgttat tttgcgatgc catatgagaa aaaatttcat
     4321 tatttatacc aaaaataggc atttgaaccc caaatctgaa ggccgctatc attatgaaag
     4381 taaaaataat tttttctttc aagcctgaag catgccacat agacattaag tctgcagttg
     4441 tgggcatttg aactttttgc tgcattatac tatctcaacc tttccgcctg ctgcttcgat
     4501 ttttgtttta gcctgaggcg taacgtaact agctttaatt ttaacagatt tggagatttc
     4561 gccattaccc agcactctta aacctactgt tgaagggtgt gcttttttgt tttctttaag
     4621 aatttcaagt gaaacttctt ttaatcctaa tttttcaatc atgccgacgt taatttcagc
     4681 ataattaagc tgattgataa gcttgaagcc ttttaatttc ggcaatcttc tgaaagccgg
     4741 catttgtccg ccttcaaacc ctctttttgc agagcgtcct gaacgctgac cttcaccgtt
     4801 gtggcctctg caacttgttt taccgtgacc tgaagaacgt ccgcgaccga ctctttttga
     4861 ttttttggtc gcaccttcgg cgggttttag atctgttatg ttcatgttta taaatccttt
     4921 ctttaagagt gagctgtgaa cggtgagcgg tgagctgaat tcagttcact gttcactgct
     4981 caccgttcac tatttcacta ctttaaccaa atgagtaatt tttctgacca tacccataat
     5041 aataggcgtt tcggcctgct ctactatttg atttgttttt gttaaaccca aagctttagc
     5101 gactttgcgc tgtgcttgtg aagaaccgat aaggcttttg ataagctgaa ttttaatctg
     5161 atttgtttta gctttaattt cagcttttgc tttggttttt acttcagctt taactgcggg
     5221 tttaactgcg attttttctt ctattttagc tgcaggtttt gctttaggct ttgccgcggt
     5281 tttaggttta gtggcggttg ttttagcctt cggtttggct gtagctttag ttttattttt
     5341 agtttctgcc atttttatat tccttttcta acttagtatt tctgcaacgg tttttccgcg
     5401 tctttttgca acttctttaa acggtcttaa gtttctaagt gcgtctaaag tagcgtttgc
     5461 ggcgttaaga ggtgatttag agcctagaga tttgcacaaa atgttttcaa cccctgcaag
     5521 ttctaataca gagcggacgg ctccgcctgc gataatccct gtaccttgag aagcgggtct
     5581 taacataacg cttcctgctc ctgcgcaccc tgtaatagga tgcgggatgc ttgttttaaa
     5641 aataggtact tctataaggt tttttctgcc gtcagcgatt gctttttgaa tagcgatgat
     5701 gacttctgct gctttggcac agccaacgcc gacttgacct ttttgattgc cgacgataac
     5761 aactgctcta aagcttagtt ttttaccgcc tttaactact tttgtaaccc ttctgatttg
     5821 aactacacgt tctttccact ctgattgaac aggtgctcct gcatcttgag tgtccacttt
     5881 ttttcttttt ttaaatttct tttcttcttg ttcttccact gttatttacc tttcgtttgt
     5941 gtaagtaata caaattaact gtctaaccta aatacatcaa aagcaagcca aaataggcta
     6001 tttgaaactg tatttaattg tcgtgatttc tgacaactat tatcgtatta caaaaagatt
     6061 tttttgcaag aggggggcag gaggaaatag gaaatagtaa atggtaaata gtgaaaaaga
     6121 agttaagtag ttaagcagct aagaggcaga aaatccgtaa ggtgggcata cttgcccaca
     6181 aaaatatcat aaaaaaaaga cgcataaagc gtcttttttt ttaaaataat actcccaggg
     6241 gaattctctt gcatcgtagg cgagcggcac gagccgtacg aggcaggatg caggttgcct
     6301 gttaatcccg tatgtttcga cctgcgggag aaacatatcg gattcgaaat tcccttaata
     6361 taatcgcact aaaaaagcgg cttattgccg cttttaaaaa taaatactcc caggggaatt
     6421 cgaatccccg tcgcctccgt gaaagggagg tgtcctaggc ctctagacga tgggagcctg
     6481 tatttccttt catcaatact aatatatcca aagaaaaatt tattgtcaag ctaattggtg
     6541 agaagtgaga ggtgagaaga aaagcaatga cgtaaaagag attcttcttc ctctccaaaa
     6601 cgatacttaa tcgttttgtc ggcagagtgc tcagaatgat gattttttgg attgcttcgg
     6661 cgatgcctcg caacgacatc cgctcactgc tcacagttca cccctcacta tttaccccct
     6721 tgaagttttt tactgtttta ggtatgatgt tgacataaat tgagaatagg agagaacatg
     6781 tttgagcgtt ttacggaaaa agcaataaaa gtaatcatgt tggcacaaga agaagctaga
     6841 agactaggtc acaactttgt tggaacagaa caagttttgc tcggcctgat aggcgaagga
     6901 acaggtgttg cagccaaaac ccttaaatca atgggggtta acctcaaaga tgcaagagtc
     6961 gaagtcgaaa aaataatcgg cagaggttca ggttttgttg ccgttgaaat tcctttcact
     7021 ccccgcgcaa aaagggtttt ggaattgtca tgggatgaag cgcgtcagct tggtcacaat
     7081 tacatcggaa ccgaacacct tttactgggc ttaatccgcg aaggcgaagg tgttgccgca
     7141 cgtgttttgg aaaatttagg cgtagattta aataaaatca gaagcaatgt agttaaaatg
     7201 ttgggtgaat caaaacctca agcggtttct tcaggggcat cctcatcttc cggaagcaag
     7261 gcaaaaacgc cgagtttgga tgaattcgga acagatttaa ctcttgcggc acaagaatta
     7321 aggcttgatc ctgtcgtcgg cagagaaaaa gaaatcgagc gtgtcataca aattcttgca
     7381 agacggacaa aaaacaatcc tgttttaatc ggtgagcctg gcgtgggtaa aaccgctgtt
     7441 gcgcaagggc ttgcaataag aattgtcaat tccgaagttc ccgatattct cgacggcaaa
     7501 aaggtaatcc agcttgacat ggggcttttg gttgcaggaa ctaaatacag aggggaattt
     7561 gaagaacggc ttaaaaaaat catggatgaa atccgtcagg cagggaacgt aatccttgtc
     7621 attgatgaaa tgcatacttt gataggtgca ggtgccgcag aaggtgctat tgatgctgca
     7681 aacatcctta aaccagcact ttccaggggt gaaattcagg ttatcggtgc aacaaccctt
     7741 gatgaataca ggaaacacgt ggaaaaagat tccgcgcttg aaagacgttt tcagcctata
     7801 attattgaag aaccgacagt cgaagaaaca attgaaatta taaaaggtct gaaggctaaa
     7861 tacgaagacc accataagct taacatctct gacgaatcaa tcgttgcagc ggctaagctt
     7921 tccagtaaat atattacaga cagatttttg ccggacaaag caatagatgt ccttgacgaa
     7981 gcaagttcaa aagtaaggct tcagatgagt tcgctttccc ctgaaggcaa agaacttgat
     8041 aaagaattgc gcgctttgat taaagaaaaa gaagacgcaa tcagaaatca ggaatttgaa
     8101 aaggcttcac agttaagaga tgatgaggct gatcttaaag agcgcatcag ggaaatgtca
     8161 gccaagtggc gcgaagagca cgaagcaaat aaaccgactg taaccgaaga gcaaatcgcc
     8221 gaagtaattg caacaatgac cggcgttcct gttacaaggt taaccgaggg cgaaagcgac
     8281 aggctgctta aacttgaaga tactcttcac gaaagagtta tcggacaaag tgatgctgtt
     8341 gtcgcgattt caaaagctat aagaagggca agagtaggat taaaaagtcc taacaggcct
     8401 atcggaagct ttattttctc aggcccgaca ggtgtcggta aaactgagct tgcaaaagct
     8461 ctagctgaag ctatgttcgg ctctgaagat aatatgatac gtgttgatat gtcagaattt
     8521 atggagaaac attcaaccgc aaaacttatc ggttcacctc ctggatatgt cggttacgat
     8581 gacggcggac atttatcgga aatcgtccgc aaaaagcctt attctgtaat cctttttgat
     8641 gaaattgaaa aagcacaccc cgatgtgttt aacataatgc ttcaaatcct tgatgacggg
     8701 cgtttgacag attccaaagg cagacacgta aactttaaaa atacggttat tattatgact
     8761 tcaaacgtcg gcgcaagtat gattgctaat cagtctaaac ttggatttac aagtgccaca
     8821 gacgagaaaa aagataaata cgaaaaactt aaagatactg ttatggaaga aatgaaaaag
     8881 gcgtttaggc cggagtttct taacagaatt gatgatatta tcgtattttc tcacctttct
     8941 aaggatgaaa ttcgtcaaat tgttgatctt atgcttaaag atttatttaa acgtcttgaa
     9001 gaaagagaac tttcaattga agttactgat gaagttaaag actttcttgc aaaagacggt
     9061 tactcggaag cttatggagc aaggccgctt agaaggctta ttcaaaagcg tattgaagat
     9121 attcttgcag aagaaatact tacaaataag tattctgccg gtgatgtaat agttcttaaa
     9181 atgcaggatg ataaaattac ctttgaatca aaaggtaaat cgtctaaaga taataaaaaa
     9241 gaagaagctg ttaagtaggc ttatacctgt cattctgaac ttgtttcaga atctctttaa
     9301 tgaaaaactt tgagctcctg aaatgaattc aggatgacgt aaatgagaac ctttttagtc
     9361 cgtttctttt atatataata aattttgttt acaaattatt tgcaatttat tatttttatt
     9421 ttattattat gttaggccga tttttgaagc gcgactagaa tgtggaaaac acattctttt
     9481 taaatacttt aaaaacacat aaacaaagga aaaggacaaa taaaatggga atcaaaggca
     9541 gagttgacaa aatggtggtt ttagcttgcg aagactgcaa agaaagaaac tacacaactg
     9601 ttaaaaacaa aaaaacgact aaagaaagaa tggagttgaa aaaatactgc ccttcttgtc
     9661 aaaaacacac agcacataaa gaaacgaaat agtaaacggt aaacagtaaa cagtaaacgg
     9721 aaattccgtt caccgttcat agttcactgt tcacccatag gcgtccttag ttcagttggt
     9781 agaacgtcgg tctccaaaac cggatgtcga gggttcgagt ccttcagggc gcgatttata
     9841 taaaagtaaa aagtaaaggt aaaacgtaaa atgatattaa gttgaaaagt ttaaaagtcg
     9901 aaaagtcgaa aagtttaatt ctaaaaaaaa ctattcgact gttcaactgt tcgactattc
     9961 aactattaaa ggaaaaaatg aacgaaacaa ttaacgtaat aactgcatat ttaaaaggtg
    10021 taagagccga atggggcaaa attacttggc cggaacgccg tcaggttttt gcagaaacct
    10081 tttttgtggt tataatagta tttgttttta cggtagcagt gtattttatg gatgttattt
    10141 ttaaatgggt attgggattt atccctcaca gataagaaag accgataggt aaaattatgg
    10201 taaaaaaaga gaaaaaagaa ttaacggaag atttactgac agaagcagtt ttggaagaaa
    10261 aacgcgaaga tgctgcgccg gttcacgaaa agctcgatag aaaagggcaa aaacgttggt
    10321 acattgttca tacgtattcg ggacacgaaa ataaggttaa agttaacctt gaaaaacgta
    10381 tcgaatatat gaatatgggc gaaaagattt tcagagttga agttcctcaa aaaaccgtaa
    10441 ctcaggttaa ggcgggcaaa aaacaagaaa aagaagaaaa gattttcccc ggatatgttc
    10501 ttgtagagat gattatggac gatgatagct ggtatgtagt aaggcatact gcaggtgtta
    10561 caaagttcgt aggctctgcc aaaaaaccaa tcgctgcaaa agatagcgaa atcaaaaaaa
    10621 ttattcacag aactcaagct caaactcaga aagttcagct tgatattaaa gcaggcgata
    10681 aggtcagaat cgtttcagga ccatttgcag aatttatcgg agatattaca gaagtttatc
    10741 cggataaatc taaactcaga gcaaatgttt ctatattcgg acgtgaaaca cctgttgaac
    10801 tggaatacaa acagattcag aaattataat tgtggaaggg ttttccccgt tagtaccacg
    10861 aaaggagaat aaatcatggc aaaaaaagta ataggtaaaa ttaagcttca aatcgaagct
    10921 gggaaagcaa atccatcacc tccggtaggt ccggcattag gtcaacatgg ggtaaatata
    10981 atggattttt gcaaacagtt taacgcaaaa actgaagcgc aagcaggcta tatcattcct
    11041 gtagttattg atgtttatga agacagaagt ttttcattca taacaaaatc accgccggct
    11101 gcagttttga tcaaaaaagc acttaatctt ccaaaaggct ctgcggttcc taataaggac
    11161 aaagtcggac aaattaccca ggctcaaatg gaagaaattg caaaaacaaa aatggaagat
    11221 ttaaacgcaa cttcattaga aagtgcggtt aaaatgatta aaggttctgc tcgcgctatg
    11281 ggcgtgacag tagctgaata gtttaatttg ggagtctctt ctcagagacg ttactaccaa
    11341 gaaaggatta taaaatgaca aaattaacta aaagacaaaa gaaaattcaa gaaatgttag
    11401 aaggctttga acagcctgct actatggctg atgccataaa aaaaatccaa gaaatttcaa
    11461 aagaaacatc aaaattcaat gaaaccgttg aatgtcacat gcgtttaggt attgacgtga
    11521 aacacgctga tcagcaaatt cgctcaacca cggttttacc tgcaggatta ggcaaagata
    11581 ttagagtttg cgttattgca aaaggtgcaa aagctaccga agcaaaagcg gcaggcgcag
    11641 atgaagcagg tgctgaagaa gttatagata aaatcgataa aggatggttt gaatttgata
    11701 ctttgatagc aacacctgat tgtatgggta tgctcggtaa attaggtaga gttttgggac
    11761 caaaaggctt gatgccaaac ccaaaaacag gaactgtaac tttggatgtt gcaactgctg
    11821 ttaaagatgc taaagcaggt aaagtcgaat ttagagccga taaacaaggc atgattcact
    11881 gtcctgtcgg taaaattcaa ttcaaaacag aagatttgct taaaaacttc gcgactcttg
    11941 cagaagcggt tattaaggct aaaccttctt catcaaaagg tacttattta aaatcagttt
    12001 atctggcaac gactatggga cccggaatta agcttgattc taaaaacctt gcctcagaaa
    12061 tcaaagaata cgtttcataa taaattcaaa tacaaaaagg tacaggtttt aaaccctgta
    12121 cctttttgta ttataattaa tataatctgt catgctgaat ttatttcagc atcttaccac
    12181 ctttgcaatt tggatagatc ctgatactat tgacaaaaat ggctaaagat tggacaattt
    12241 acatgtaccg taaggacatt ttccacgaaa tcaaagattt cgggaaaaga agtcaaacga
    12301 attcaggatg acaagaagat ggagatttat aagcatggag aagggattat ttataacatt
    12361 tgaaggtgct gacggctgcg gtaaaacaac gcagatgaat cttttgcatg aatatttgat
    12421 tttaaaaggg tatgacgttg ttttgacaag agaacctggc ggcaaaggtt taggagagag
    12481 aattcgtgag atacttttaa actatgaagg tgaagtttct tcacaagctg aggctttttt
    12541 attccttgct gacagagcgc agaacattga tgttattgta aaaccggcag ttgatgcagg
    12601 aaaaattgtt ttatgcgacc gccatattga ttctacaatc gcttatcagg gatacggcag
    12661 gggcgttaat ttagagcgtc ttaaaatgct caatgacatt gcaacaaata aatttatgcc
    12721 tgatttaact tatgtttttg atatagatgt ggaaacttct caaagccgag tcggtaaaaa
    12781 caaagacaga atggaatctg ccggagtaga attttttgaa aaagtaaaaa agggctatct
    12841 tgaattggca aaaaaagaac ctgaaagagt caaagtgatt aactcagcaa acacaataga
    12901 aaatattttt gcacaagtta aagaaatttt tgaaaaaact atttgttaaa ttccaaaaac
    12961 atttccttga gcaaattata gccttgaatt aacgttttgt aatctatttt ttcatcgatt
    13021 gagtgcatgt taaaaacatc tgcaagtccg actaaaaacg ggcaaatcgt ttcgcccttg
    13081 gagtttttat tttgtgcgta aatatgggtt tccgcacctg catgaaatga tgaaatatcc
    13141 tgatttatgc ctaacattgc gcatacttta gtgtgaattt tttctataaa atcgtcctga
    13201 gccttttcaa acgggggcaa atgctgagaa aatataattt cggcatttgc aagcgaatca
    13261 tattttttat tgaatttttc gacgatattt tgaataattg cctttaattc attttctttt
    13321 tcaaagcttg cgcttctgat agaataagtt gcctgagcct ttgtatttat aacatttgtg
    13381 acaggagtgc cgttttgagt attacccgcg ataattgtac cgatattaat tgatgttatt
    13441 gtgccggttt catctttgta aaatactccc tgcgggattt cattaaccaa ttctgcaatt
    13501 aattttgccg cattaagtct tttttcatct cctatatcaa gcccagaatg tcctccgtat
    13561 gaagttttca aattaagttg agcatttgtg tagcttgcac ctgatacaag tagctgacca
    13621 agtttatctg catccaagac aagcgcgtat tttgaattta actttgaaaa atccagatgt
    13681 ttaatcccgc tcattccgtg ttcttcgtct cgggtaagca caatctctaa aggataaatg
    13741 tgtgaaaatt cataagcaag catcaatgca caggcgactc ctgctttatc atctgccccg
    13801 agagttcttg ttttgtcagt ttcaatgaaa tcatcgttaa tttgaagatt aatcggagaa
    13861 gcatctccca caacgtccat gtgtgcggat aaaagcaagg gctgtttgtc tgttacagtg
    13921 ccgcttactg ttataatcaa atttccgtaa tcatcagatt tacaattgat accgttatcg
    13981 gcacaaaaat ttttaatcca ttcaattact ttttcctctt tcattgaagg agaaggaatt
    14041 tgcactaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta atccattcaa
    14161 ttactttttc ctctttcatt gaaggagaag gaatttgcac taattcgcaa aaaatttcta
    14221 ataatttatt ttcttttttt aaatcttcaa tctgcataaa aaatcctttg atttgtacca
    14281 ccttcaccta ttggtattct tacgctgcga gagcatttcg gacatcttcc gacataagcg
    14341 tcaccggttt tgtttttata aattcttgta taaacattac agcatttaaa cattatgcca
    14401 aggtaaggtt ttttttcatt tttttcgaat ccgctcatat ttatccactt tgccccattt
    14461 accccttaaa taatttttct taacttaaac ggtgtgcgtt ttttcctttg aaattgatag
    14521 cgtgtatttg gctgaatttt ataggtgtca tcttccgttg gaagaaagac acttgccata
    14581 agcgtatttt tatatttatc gtcaaggtgc gcataatcaa aaagtattat accttttgat
    14641 tcgagttttc gggcttcatg gatttgcctt attaaatctt catttgagcc tcccataaaa
    14701 gttacaaaaa tccctgaata aagttttgta cagtcacatt tattacgatt tatatcttgc
    14761 atcattatat ttgcggtttt tgcgtcgcaa gttaaaagaa gcggtgtaaa tccgtctata
    14821 taacctcgtt gtgaccaagt tttccaatct tgttgttttg tctctaaggc tttttgcctg
    14881 tcagggaaaa taactgctgt taaaaggata tttttattgt gtgtaagctt acttattcgc
    14941 tcgacaaatt ctgttatatt gtcttgtctg tatttgtccc aaacctgcca taattcatcg
    15001 tcaggaagaa tttctacagg gtctttgcaa tattgggatt taaaatccgc tcttgcatat
    15061 tctgtgtaac cccaatttga caaatcatag ccgggatatt ttgccgctaa agattgcgga
    15121 taacgtatgt agtcaagatt tatgccatca ggagaataat ttgtaataat ttcttttatt
    15181 aaatattcca aaaagtcttg tacttcagga tttgcagggt ctaaaaaata tccgttatgt
    15241 tcggacacag aaggtgtggg tccttgtttt tcgtaatctt tttttgtttt atttgcccat
    15301 gcgggtttta ctgctaaaat gcttttggaa ttagaatttg ggggctcatt tcctacataa
    15361 aaagtttcaa accaaatatt gactttaatt ccgcgtttat gtgcctcttg aatccaaatt
    15421 tttaaaggat caaagcctgc aaatttttcg ttttgcctga taaaattata atctgacatt
    15481 gttttgcttg gaaaaatagt tttgccatga aaataagttt caagaaaaat attgttaatt
    15541 cctgaagctt ttaatctgtc aagtgttttg gtgatttctt cggatgaagt ttcagtcgga
    15601 cgtatccaaa cccctttgag ctcatcagct ttgtacggaa gtgcatttgc aagtgccaaa
    15661 tttgcgcttt ctattgcctg tgaagaatat ttttgaacat ttgaagggtc atgctttgct
    15721 ttttcaagat aatcttgtgc ttgttcaata tacattttag gttttttggc atcataataa
    15781 agggaacgtt ctttgtaata atttacgatt tcatttgctt cttttatttt ttgttgggca
    15841 ccatagagaa aactgtctga agtgatataa actgtaagtg ttttgtctgt ggggttaaca
    15901 tatacttttg aacctaaaat gacattttca ttaatccatt tttttgcgct tccgtgaccg
    15961 cttattacaa atccgtctcg agggattaga gaatcggctc cgcctagagc cgttgttatg
    16021 tttttgcata cgatagcttc agagccgaat tcgtttgtgt tggttctgaa accaaaagcg
    16081 ggagtataga ctatcatttg gtttgcgcct cttaaacccg gataaaagct tcctaccgtg
    16141 tttgaaacaa tagtcggatc tatcgcatta agcggataaa ctttttggct gaaaattatt
    16201 tcgctgtcgt cagaaaagaa attaaaatca tccgcgaaag caggacaaac aaattgcatc
    16261 aaaagtgcaa acaatataaa taatttccaa agcactcggt tcatctatcc cttcccttgt
    16321 acatgtcttt gattttatta tcatagctta attgtactgt ttttatgtcc tataacagga
    16381 gtattttatc aggttaaatc gtgaaaaaaa tcatcttaat aaaacacaac aagtactatg
    16441 ctagaaaaaa tgttttgatt attataatta taggtacttt tgtaagaggt tagattgaaa
    16501 gagctgattt ttttagcggt atttgtggtt ttgtcagggc tttttgctct tgcagcactt
    16561 attgcaagct atatctgctc tccaagaaca ctgtttgaag ataaaaaaac tcactatgaa
    16621 tgcggcatgg aattgttttc agatgccaga atccaatttg atgttaaatt ttttaatttt
    16681 gctattatgt ttttaatctt cgacgttgaa acaatttttc ttttcccttt tgccgttaat
    16741 tttaaccagc ttgaagcatt cgcccttatc gaagtaacaa tttttgtatt tttactgata
    16801 tttgcacttt ggtttgccat aaaaaaagat attttgaggt ggcagtgaac gttataattt
    16861 caagcataga ttttatttat aactggggac gctcgaactc tatttggcct atgacttttg
    16921 ccacttcatg ctgcggaatc gaaatgatgc aaacttctgc agccaatttt gatatcgcgc
    16981 gtttcggctc ggaagttttc agggcaaccc caaggcaagc ggatttgctg atttgtgcag
    17041 gaacaattac ccataaaatg gctcccgcca tgcttaaatt gtaccaacag atgcccgaac
    17101 ccaaatatgt catcgctatg ggagcatgca cttgtggcgg cggaatgttt ggcgacagtt
    17161 attcggtggt taaaggtatc gataaggtaa ttcctgttga tatttacctt ccgatgtgcc
    17221 cgcctacgcc gagcgcgctt ttagatgcaa taattaagct tcagaaaaaa atccgcaaag
    17281 aaacaatttt gaacagaaaa aaatatattg atgcccacgt ttctactcta aaacttgatg
    17341 aagaaagtga aattttaatt aaagattccg gtataaatat tcaatactgg ggagtatcgg
    17401 gggatttatc agatgaactt tgaagcgttg caaaattttg atattcaagt aattaagcaa
    17461 gaaaattctt tcgacaaaat tcgcgtggat aaatcgaatt tagttgacct tttgagcttc
    17521 cttaaaaata atgctgaatt tgattttgat acccttttat cgcttatcgc cattgatttg
    17581 aaagattcat ttgaattgat ttattgtgtt tattcgacaa aattgaacac tcaaaggcag
    17641 atttctgtcc ttttggacag aaatctgccc aaagtaaatt caatcgtaga tgtttataaa
    17701 agcgcatatt ttgatgaatg tgaaatttac gatttatttg gcataaattt tgaaaataat
    17761 cccgatttaa aacgtctttt tatgccaaaa ggctggattg gaaacccttt gcgcaaagat
    17821 tatattgcag atgattcaag attagcctgg aatgaggtga aaaatgccta attcagagcg
    17881 cacaagaatg aagataaaca tagggcctca gcacccttcg actcacggag ttttacgcct
    17941 tgtacttgaa cttgaaggcg agattattaa atcctgtgag cctgttatcg gctatcttca
    18001 caggggtatg gaaaaaatgg cggaaaaccg cacttatttg cagtatcttc cgactgttga
    18061 cagagttgat tatttaagcg gattctttaa ttcctacgcc ttttgtgatg cggtcgaaaa
    18121 tcttgccaaa attgaagttt ctaaaagaac aaaatatatc agggtattga ctcttgaatt
    18181 aaaccgtctt gcctcgcact tgctttggtt gggtacttat atgttggatt tgggtgcaac
    18241 aactcctctt ttttattgtt tcagggatag agaaatcata ctgcacctgt ttgaggaact
    18301 tacagggcag cgtttaatgt ataattacta tacttttggg ggcttaaggc acgaagtttc
    18361 ttccgattgg cttaataaag tcaaagattt tgttaagatt atgccttcca gaattaatga
    18421 ttacgaagga attattacca ataatcctat ttttgttgct cgcgcaaaag gagtcggaat
    18481 aatttctaaa aacactgctt tacaatattc tatgacagga cctaacctta gggcaagcgg
    18541 gattaatgtt gattttagaa aacaaaaacc ttatctggta tatggcgaca ttgaatttga
    18601 cacagcagtt gctcaaaacg gcgactgcta cgacaggtat aatgtcagaa ttgaagaaat
    18661 gagaacttgt ttaaaaatca tctctcaatg tgttgattgg cttttggtta atcaaggtga
    18721 agttttatcg accgtaaaac aacatttgct taaagttccc gaaggcgaat acatttccaa
    18781 tgtggaagca cccagagggc tttgcagctg ttatgtcaaa tctgacggtt catccaatcc
    18841 ttacagggtc aaatggcgta caggctctta ttattctgtt caaattttgc ctaaattgct
    18901 tattgattca atgtatccgg atttaatgac aattttcggc tcccttgatg taatcttgcc
    18961 ggaggttgac agataatgga ttatttatat ttcttatatc tgaatttgtt ggaaaagttc
    19021 catatacctg cttttgtggg aactgtaact ttccctgtgc ttccgttttg catagttgcg
    19081 gttgttttaa ttcttgtagt cttgtttttg gtgctgcttg aaagaaaagt tttagctgtt
    19141 tttactcaaa gaaaaggccc taacagagtc ggtccttggg gcttgtttca aataatagca
    19201 gacgcgataa aacttctttg caaagaaaat atcacgcctg aaaaaagcga cagattcctg
    19261 tttatgattg cgccgctact cgcatttgtg cctgtaatgg ttgtttgggg attgattccc
    19321 ttcagctctg aatttgattt tatatcttat tcggtaagtg gtctgctttt catttctata
    19381 gcttctattc ctatcatagc aattctttta gccggttatg caagtaacaa caaatattca
    19441 cttataggct caattcgttc tgtagtgcag gcaatcagct atgaacttcc gatggtattt
    19501 tcttttttaa gcattgtttt gcttgcatct tctatgaatt taaaacaaat agtttttgct
    19561 caaataagca tatatccgtt ttgcggatgg tttattttcc ctgccttttt aggctttgta
    19621 gtaatgttta tatgcgcgct tgcagaatta aacagatgtc cttttgacct gcccgaggca
    19681 gaaagcgaac ttgtaagcgg ctataacaca gagtattcgg ggatgagatt tgcgtttttc
    19741 ttccttgcag aatacgctat ggcatttatt atgtgcgcat ttcttgcaac attgttctta
    19801 ggcggatttt tatcgcctct aggattttat atttccgaaa aaatcttcgg gtacaacact
    19861 ctttctcaaa ttttagttta ttttgagcag gtattttgga ttttcttaaa aacttttgcc
    19921 ctgtttattt gtattttatg ggtaagagca actttgccaa gacttaaatc agaccaactt
    19981 aacgcttttg cgtggaagtt tttactgcct gtttcaattt taaatctctt
//
LOCUS       DAOQAU010000031        19665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2876_length_19665_cov_7.574605, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000031 DAOQAU010000000
VERSION     DAOQAU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 19665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19665
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_19665_cov_7.574605"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKI94_05660"
     CDS             <1..608
                     /locus_tag="PKI94_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNW26261.1"
                     /translation="PQNPCQNCEKSGCNLKGEIFEEFPPNCAYKNWQKEVLKYLQEDV
                     AKKIYQKWIKMLEYRQKFSCSGCATCCKLACSEFSYEELKQKAKEGDNFASQFVSIFV
                     PYKTKEEAQKIYPEYIELLEEKLGKDEKVYFYFCPKLDENNRCSDYQNRPQICRDFPD
                     NPLSMLPKACGFSKWKDEVEPTALMLHSTLEIVDFYKNKIK"
     gene            616..1635
                     /gene="rlmN"
                     /locus_tag="PKI94_05665"
     CDS             616..1635
                     /gene="rlmN"
                     /locus_tag="PKI94_05665"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNW26262.1"
                     /translation="MEKIILSGLSQKELEKITEKLKASKFRARQIHNWIYLKSAKSID
                     EMSDLSKDFREKLSEIAQISNVKIKTKQTSQDGTIKYLIEYLDGNCVETVLMRFDNRA
                     NLTACVSSQIGCAVNCAFCATGKSGFVRNLTAGEMVEQVLTIQRDTGLKVTNVVFMGQ
                     GEPLLNLDNVLDALKIFNEDFQIGARRITVSTSGIIPQIEKLAQLELQSTLALSLHAP
                     TSELRAKIMPIEKKYPLDELKKVLKNYTQKTGRRITIEYVLIKDFNDTPEVAKQTAFF
                     LKDIKCNINLIPYNPVDKTGFQKPAESSIMKFKYILEHCGKKVTIRLERGADIDAACG
                     QLNSK"
     gene            1720..3666
                     /locus_tag="PKI94_05670"
     CDS             1720..3666
                     /locus_tag="PKI94_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNW26263.1"
                     /translation="MSDNPILDKNLECIGIYNPQLVQDILKITALTGSFELVKTNLGE
                     PNLIHNGLPIHSQEGAEAEAKGIFAKIQNSALSIHIVYGLGLGYLFKEFCENSKGKVI
                     LFEPNLEILRATLELVDFSKELSQPNVKVTSEISNFKEIFSREYTYKAKSTISFLVSY
                     KNNYKNKIDEFFKEVEIFSGICTADYNTLKMAIMESVNSTLENFIYTLDETPLWEIKD
                     SYKGKTALIVSAGPSLDLNIETIKANRDKFIIFCVGTALKALMKNGIKPDFLNILEVH
                     DCSSQVAGYDLSDINLILEPYTHNIFHKLKVKQKFIFPTNSGHSSIWWSKITGVDISP
                     YFAKGTVSYEALCSAKMLGFSKIILVGQDLAYVNNQCYSKDAAYSELVYDVNPETKKV
                     QIKINDYEKYIQSLIPESGENPNVDYRGFASYKITNLNDTLYFVKGVKGDMLPTQGGY
                     ATFIEHFKDFASKNPDLDLINSSMIGANIEGFKNISLEDSIKETPFILEKPNLSKQNR
                     SYDKQSIFSSLEREEVLLKEILKEFEQAREYIFKYDREFRRRRTLSEETNKYLKLLLN
                     LYNKILLEYQGNNILYQAISFNENLEVQFALKSSENVNIVEIQNVYEQLKIYFYMVEE
                     KLTKTIEKIQQQKGKVLESINSTG"
     gene            3641..4078
                     /gene="dtd"
                     /locus_tag="PKI94_05675"
     CDS             3641..4078
                     /gene="dtd"
                     /locus_tag="PKI94_05675"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNW26264.1"
                     /translation="MKALIQRVKKASVKIDGKLYSQIKSGLLVFLGVSKSDTKENAQK
                     LAQKVLSLRIFEDEHGKMNKSVLDVQGEILIVSQFTLLGDCKKGTRPSFDDAASPENA
                     KKLYEEFVKIISDSGTRVKTGKFQAMMDVSLINDGPVTFMIEK"
     gene            4263..5387
                     /locus_tag="PKI94_05680"
     CDS             4263..5387
                     /locus_tag="PKI94_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26265.1"
                     /translation="MYVNKCTKCGAEFETKNPKRVICPDCLYPDKKMMLSSEIEQEEV
                     IEKPQEVDQGNNQHEEPQKQKFYSSYSYETPQQQHPPRQNNYLRRDNYSDRGNYQRRD
                     NYGDNRGGYQRPQQGGYQRPRQDGYQRPQQGGYQRRDNYGDNRGGYQRPQQGGYQRPQ
                     QGGYNRPQGGYQRPQQGGYQRPQGGAPRFGGNRPRPPINKNKPLLVNKEQLEQIELLY
                     KAMLPLPNPDAHEVIGAKIELEPRKVFFGINLIRQKLMLPKLPFPKRKLAISPDQMFA
                     IQNLYEPLLPLPPIGCHKIIAAQLKMDEWRVHVGIGLIRAKLGLSRWNQERADAPEEF
                     KKAAKEKVSKAKKTKEEKPEKKAKEPKAKKETKAKKESKK"
     gene            5399..5911
                     /gene="rimM"
                     /locus_tag="PKI94_05685"
     CDS             5399..5911
                     /gene="rimM"
                     /locus_tag="PKI94_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNW26266.1"
                     /translation="MDKYVSVGKILNFHGIQGEAKVGFSKNQADFLKSLKSAFIKFEN
                     EYKLFKISSVRFNKNFALIKFQGINSINELIPYKNCLIFVERQTAREKLEENEFLIDE
                     LTGLDVFDQNNENIGIAVGVSNNGINDFISVKSKSKNICLVPFVKALVPEVDIKNKKI
                     IVNNIKGLIE"
     gene            5908..6591
                     /gene="trmD"
                     /locus_tag="PKI94_05690"
     CDS             5908..6591
                     /gene="trmD"
                     /locus_tag="PKI94_05690"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNW26267.1"
                     /translation="MKFDVLTLFPEMIENCCSHSILKRACQNKVIKINTINPRDFTHD
                     KHKKVDDKPFGGGAGMVLMAQPFVDAYESIEKEKNSITVMLTPQGKVFDDKMSNEFAK
                     YDQIILICGHYEGFDERIREIIKPQEISIGDFVLTGGELPALCLIDSISRKIEGTLGK
                     QESAEFDSFSNGLLEYPQYTKPRNFKGLEVPEVLLNGNHSQIEKFRHEQSIKRTKAKR
                     KDLWDKYSN"
     gene            complement(6588..7187)
                     /locus_tag="PKI94_05695"
     CDS             complement(6588..7187)
                     /locus_tag="PKI94_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26268.1"
                     /translation="MEQYFIGLSLAASANTESGVVVIDKNNSIITLDKLFSMQDIQFF
                     FDNFNSLKNAYICVSLPWDNSMLNGKWRILSKPYQLLNSGEKLINTDNWTQRYSNRGS
                     DYFKILLEKGIKISRFELYLTRQALGLESGFKERTPADCKSLQNALKTKYGFDEIPPN
                     MMPMAQLEAFVGAILAKRMSERKTSESIFEFNGLPVIRS"
     gene            complement(7188..8300)
                     /locus_tag="PKI94_05700"
     CDS             complement(7188..8300)
                     /locus_tag="PKI94_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNW26269.1"
                     /translation="MARKQKHPEHENLERWLISYADFITLLFATFVVLYALSQVDVAD
                     FKKLEESIKQAFSAPSVMQGADNIMQDKSQEIFDKSEGETLITPLMMEYMSQKYEEQS
                     FDDIQKTIDDMNKNGEIDGVEATMTDRGLLIRFNDNYLFKPASATLTPQAKQKLDKVG
                     AVIGKRFFLHNIRVEGHTDNQKIVSIVFPSNWELSSARSCSIIRYLIERFGFMPGLFS
                     AIGFADTRPIASNRTPQGQFKNRRVELLILKNQFKAMEVPQNNIMKMSKKEQEELQKR
                     RMETINTINVITNTSDKSFDADKRAREQAAILNATYEKELKRISKETQVLDDPTKTKI
                     TGQGSWLKPPAKPAQSQGGKTALPRYVENEFKNVGR"
     gene            complement(8302..9057)
                     /locus_tag="PKI94_05705"
     CDS             complement(8302..9057)
                     /locus_tag="PKI94_05705"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNW26270.1"
                     /translation="MDIFAIVGVVVGLGFILAAQALEGGNIGQLLQVTAGMIVFGGTA
                     GAVIASFPVPDLKNGVLMLKDVFFPPAVNFENLISEIVGFATKARKEGVIVLEKEAKF
                     ASDPLLTLGLEAVSDGADPSLVQGMLENQITQLEEKVNKGSKVWESAGGYSPTLGIIG
                     AVMGLIQVMQNLSDPSKLGAGIAVAFVATIYGLFAANLVLIPCSTRIKFNYQKVFVAK
                     EIMLEGVLAIQAGESPALIERKLQSFLLNNQGS"
     gene            complement(9226..9972)
                     /gene="map"
                     /locus_tag="PKI94_05710"
     CDS             complement(9226..9972)
                     /gene="map"
                     /locus_tag="PKI94_05710"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNW26271.1"
                     /translation="MIIRKSKEEIKRIRHAGYIVALVHQEMKRLIEPGISAKELDNVA
                     FDIIKKNKAVPTFLGYHGFPASICASLNEQVVHGFPLESVILKEGDIISVDVGATYGG
                     LVGDSAWTYPVGKISSEKERLLKATEEALFAAISQMFHGNILDNVGGAIEDIAKREKL
                     GIVRQYGGHGVGRSMHEEPFVFNYRTCDKTVLKSGMTIAIEPMLNLGGDAVEVLADKW
                     TVQTVDKMPSAHFEHSILVTDDKPVILTTL"
     gene            10114..10518
                     /locus_tag="PKI94_05715"
     CDS             10114..10518
                     /locus_tag="PKI94_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26272.1"
                     /translation="MQVQRVNMNSQSFGAIRFVPRDLKKALKMIDNSDFQTLIKMTKS
                     NNKAESNAANMVFDWVNGIEKLKKNGYEITEELIDYTRKMAIKRLGKNGTDGITAGLY
                     NYTLGRLDGCWLNSSILKEFRKKGQFLWPPAK"
     gene            complement(10590..11243)
                     /locus_tag="PKI94_05720"
     CDS             complement(10590..11243)
                     /locus_tag="PKI94_05720"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNW26273.1"
                     /translation="MKKELVFLGPPACGKGTQTSKLALHLNFPHVDTGSLLRKAMADK
                     TSDGLIAQSYVEKGQLVPVDIVASIIKSRLKESDCKSGFILDGYPRSTEQAILLEEML
                     KEIDAGEEVDFKVIYFDIDQKLLMERIIYRRSCAKCGKIYNLLSLKPRKDGVCDECGC
                     ELIQRKDDTEEIAKSRFDTYFEQTAPLIKFYEQKGSLKKIDANGSVDEVWDRLLSAI"
     gene            complement(11347..13797)
                     /gene="gyrB"
                     /locus_tag="PKI94_05725"
     CDS             complement(11347..13797)
                     /gene="gyrB"
                     /locus_tag="PKI94_05725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNW26274.1"
                     /translation="MTMQTEHSNYDASNIRVLEGLEAVRLRPGMYIGSTSQRGLHHCI
                     YEIVDNSIDESLAGYCSEIKVMVNPDHSITVEDNGRGIPVDIKPDSGKSALEIVHTVL
                     HAGGKFGDGGYKVSGGLHGVGASVVNALSEKMIVEVSRDGFVWRQEYLRGEPSSPVEK
                     TKETSKTGTKTTFWPDPEIFTETTEIDKETIANRLREMAFLNKGLKIVFIEGKTLEEE
                     AFHYEGGIGSYVEFLNKNKTVLFEAPVYMEKTVEGILVEVAMQYTDAYNESVLSFANN
                     INTHHGGTHLTGFRNAITRVLNDYARKNNILKDSEQNLTGEDVREGLTAIVSVKLENP
                     EFEGQTKEKLGNEEAMGAVQDVVRDKLQEWLEFNPKNAKIIIEKTLQAQRAREAARKA
                     RELTRRKSALENSTLPGKLADCSNREPEKCEIYIVEGDSAGGSAKQGRNRMFQAILPL
                     RGKILNVERARLDKIYGNNEIQAMVQAFGINISKNEDEVEIEKLRYHKIIIMTDADVD
                     GAHIRTLLLTFFFRYAKPLIDNGHIYIAQPPLFKVTTGKTSEYLYDERALDKMLRERG
                     VKGLCLCDKSKQKMCSDKDFTDLMANMSTYYNSYNNPILNAIPAVVLRGLVRSGLEAE
                     DFDNQSKMDEVIDYLNRYLQDHARNYNVKEAEKYNVSLKYNPENAKYNLVLNLGQDVE
                     AVLITSNIIKSSEYKRLKTSYPLIRNFLIEEEEKLILETDSEQIEISSFAELQRIVEE
                     RGQKGLQIQRFKGLGEMMPQQLWETTMDPATRTLLKVSIEDAMLADQLFDILMGDKVE
                     PRRDFIEANAVYATNIDT"
     gene            14050..14730
                     /locus_tag="PKI94_05730"
     CDS             14050..14730
                     /locus_tag="PKI94_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26275.1"
                     /translation="MSKVSSIDYPAFTNGQVSINGNPVASATMSNGVINSNYNMTDAE
                     NYIYNYAQNALASILPQLNVFSPDTVNSLQSQIDAYTNQGVNTINNIYSPMIKNLQND
                     IASRFGNFDNSIFMDNLKSIESKRSDAVSSFAQDIMAKRNELINNELSNRYNYADFLN
                     QIQNQTINNILSYINTALGSSSSANSYNNNLYNALYKQALAGQNSTPNLSNLSSYLAN
                     SLGINSFL"
     gene            14852..16357
                     /gene="mnmE"
                     /locus_tag="PKI94_05735"
     CDS             14852..16357
                     /gene="mnmE"
                     /locus_tag="PKI94_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNW26276.1"
                     /translation="MNILQEFDTIAAIATPIGTGGVGVIRISGGKCDLDSCDNPSSFV
                     GESKCFEIVEKIFSGKKLIAGKIHHGWILDNDKRIDEVIVLPFKAPNSYTGEDVVEIQ
                     CHGGINVVRNILDLVLKQGARPAQRGEFTKRAFLNHKLDLSQAEAVCDLIHAKTQNFA
                     IHSAKNLSGVLTQKIGEIKKDIFEVLSKILAGIDFPDDVKEPEYEYLQKEFQNALDKI
                     ESILSSAKSSNVLRQGIKIAILGKPNVGKSSLFNALLSMERAIVTDIAGTTRDILTET
                     IDIEGIPVTLIDTAGIREHSTADKVEKIGIEFSKQSADEADLILFLYDASTGMNKDDE
                     AILDLIKEKNHIKIANKADLYTDNYPSSVLWTSSPARGEEIIPSPLAGEGNFSMRDTS
                     IRKMGEGSSIYLSTITQQGFDQLKQKIKQFAGKCSSEELEFTTNTRQQDCLERAKEAL
                     TNALAAAQKEELQDLISIDVKSALLSLDEITGEVITDDILNNIFENFCIGK"
     gene            complement(16368..16877)
                     /locus_tag="PKI94_05740"
     CDS             complement(16368..16877)
                     /locus_tag="PKI94_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26277.1"
                     /translation="MQVNKISFQGRRLPESTILEVRKLLNKLGKGTKKVGSKDFGFSL
                     KWDTGLNSLKGKFINEGALPGFEPRVKISTDEFALTLNSKTGELFDIENPENLPFAKL
                     YKRVGKFVKYLNKNFSNRDVVEKSSFRVRTAGQKTIEELDAIKVNYKKPSFFTGFLNW
                     IQDMFENTR"
     gene            complement(16973..17548)
                     /gene="minE"
                     /locus_tag="PKI94_05745"
     CDS             complement(16973..17548)
                     /gene="minE"
                     /locus_tag="PKI94_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNW26278.1"
                     /translation="MREIITELYKKLAGFFQTPEKENLKEVACNRLKLVLMQDRTNLT
                     PQLLERMRGEMIDLLSKYVEMDKEALELNFAQEGDSMALMLSIPVVRAKDEEEIQALI
                     KEEEERKAKAEAEKAQEEEEETKENKDEDSEEEPKEEISEEEEKDSEEEVEEGCACLI
                     EGPCECQANENPEKEEQAWVAPKKNKKHKPQ"
     gene            complement(17554..18366)
                     /gene="minD"
                     /locus_tag="PKI94_05750"
     CDS             complement(17554..18366)
                     /gene="minD"
                     /locus_tag="PKI94_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNW26279.1"
                     /translation="MSGRVIVITSGKGGVGKTTTSANIGTALAKAGQKVVLIDTDIGL
                     RNLDLLLGLENRIVYTIVDVVEERCKLKQALVKDKKNPNLCLLAAAQTRDKSAVNQEQ
                     LKAICDELKEDNDFILVDCPAGIEQGFQNAVAGATEAIVVTTPEMSAVRDADRIIGLL
                     EAKEEIESYRLLLNRVRPNLIKSNDMMSVEDVVDILSCSLIGIIPEDTGVITSTNKGE
                     PIVNDEKSLAGQAYSNVAKRVMGEEVDFLNLEEPKGWAAKVKKFFAGLFGKE"
     gene            complement(18455..19330)
                     /locus_tag="PKI94_05755"
     CDS             complement(18455..19330)
                     /locus_tag="PKI94_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNW26280.1"
                     /translation="MTKGFVENGFIIDLGNAKDASDIIYELSSILEMPEAQYRQICLK
                     LGNIDLTQSQLLSIKSLITSLNSELAFVDTNSNQTELAALNLGLIVSKLSNEIEVVED
                     EQVQDYLFEDNSHEAVSENLYEKIEPEQVEEKIEVIEKTDEELQAEDKLEELIDEENE
                     QDKQKEESEHEVSKMSTLYLQQTLRSGQTISYDGNIVLIGDAHPGSEIIAKGDITVWG
                     ILGGIAHAGARGNDWAKIRALKINAIQLRISGFYARRPDSLNIPFIQKTSQFTPEEAK
                     VNEHGEIIIYKLYSE"
BASE COUNT         6436 a         3718 c         3323 g         6188 t
ORIGIN      
        1 agccacaaaa tccctgtcaa aattgcgaaa agtcaggctg caatttaaaa ggcgaaattt
       61 ttgaagaatt cccgccgaat tgtgcttaca aaaattggca aaaggaagtt ttaaaatatt
      121 tacaagaaga tgtcgcaaaa aagatttacc aaaaatggat taaaatgctt gaatacagac
      181 aaaaattttc ctgttcgggc tgcgcgactt gctgtaagct ggcatgcagc gaattttctt
      241 atgaagaatt aaaacaaaaa gcaaaagagg gcgataattt tgcaagccag tttgtgagca
      301 tctttgtgcc ttacaaaaca aaagaagaag cgcaaaaaat ataccccgag tacattgaac
      361 ttttggaaga aaaattgggc aaagacgaaa aggtttattt ctatttttgc ccgaaactgg
      421 atgaaaataa ccgctgctcg gattaccaaa accgtccgca aatatgcagg gattttcccg
      481 ataatccgtt gagcatgctc ccaaaagcct gcgggttttc aaagtggaaa gacgaagttg
      541 aacccacagc gcttatgctt cattcgacat tagaaattgt ggatttttac aaaaacaaaa
      601 taaagtgaaa gaaacatgga aaaaataata ctttcggggc ttagtcaaaa agaactggaa
      661 aaaataaccg aaaaattaaa ggcatcaaaa ttcagggcaa ggcaaattca taactggatt
      721 tatttaaaaa gcgcaaaatc catagatgaa atgagcgacc tgtcaaagga tttcagagaa
      781 aaattgtctg aaatcgccca aatttcaaat gtaaaaatca aaacaaaaca aacatcacaa
      841 gacggcacta tcaaatattt aatcgaatat cttgacggaa actgcgtgga aacagtatta
      901 atgaggtttg acaacagggc aaatttaacc gcctgtgtaa gctcgcagat aggttgtgcg
      961 gttaactgcg cattctgcgc aacgggaaaa agcggttttg tgcgtaattt aaccgcaggc
     1021 gaaatggttg aacaggttct tacaatccaa agagatacag ggcttaaggt tacaaatgtg
     1081 gtttttatgg ggcagggcga gccgctttta aatttggaca atgttcttga tgctttaaaa
     1141 atattcaacg aggattttca gataggcgca agaagaatta ccgtttctac aagcggaata
     1201 attcctcaaa ttgaaaaact cgcacagctt gaacttcaat ccacgctggc gctttctttg
     1261 catgcgccga cctctgagtt aagagcaaaa attatgccca ttgaaaaaaa atatccgctt
     1321 gatgaactta aaaaagtgct taaaaattac actcaaaaaa caggaagaag aatcacgata
     1381 gaatacgttt tgataaaaga tttcaacgac acgcccgaag tcgcaaaaca aaccgcattt
     1441 ttcttaaaag acattaaatg caacataaac cttataccct acaaccctgt tgacaaaaca
     1501 ggatttcaga aaccggccga aagttcgatt atgaagttta aatacatact tgaacattgc
     1561 gggaaaaaag ttacaataag gttagaacgc ggcgcggaca tcgatgcggc atgcggacaa
     1621 ttaaacagta aataggaaat aggaaatagt aaatagtaaa taggaaatcc gttaaccgtt
     1681 tactgattac aatttgccaa tataaaaaag gagcaaacat tgtcagataa tccgatttta
     1741 gataaaaatt tagaatgcat aggaatttat aaccctcaat tagtacaaga tatattaaaa
     1801 ataacggctt tgacaggaag ttttgaactt gtgaaaacaa atctcggcga gcccaattta
     1861 attcataacg gactgccgat tcactctcaa gaaggagctg aagctgaagc aaaagggatt
     1921 tttgcaaaaa tacaaaattc tgcactttct attcatattg tatatggttt gggtttgggc
     1981 tatcttttta aagagttttg cgaaaattca aaagggaaag ttattctttt tgagcctaat
     2041 cttgaaattt taagagcgac tcttgaattg gttgattttt caaaagaact ttcacaacct
     2101 aatgtaaaag taacctcgga aatatcaaat tttaaagaaa ttttttcgcg cgaatacact
     2161 tataaagcaa agtccacaat ctcttttcta gtgtcataca aaaataatta taaaaacaaa
     2221 attgatgaat ttttcaaaga agtggaaatt ttttccggaa tttgtaccgc agattataac
     2281 acgcttaaaa tggcaattat ggagtccgtg aattcgacct tagaaaattt tatctatacg
     2341 ctggatgaaa caccgctttg ggaaataaaa gacagttata aaggaaaaac tgccctgata
     2401 gtttctgcgg gaccttcttt ggacttaaac atagaaacga taaaagcaaa cagagataaa
     2461 tttataattt tttgtgtggg aaccgctctt aaggcattaa tgaaaaacgg gattaaacct
     2521 gattttttaa atatacttga ggttcatgat tgttccagtc aggttgcagg ctatgattta
     2581 tctgacataa atttaatttt agagccttac actcataata tttttcataa attgaaggtt
     2641 aaacaaaaat ttatttttcc gacaaattcg ggacattcaa gcatttggtg gtcaaaaatt
     2701 acaggggttg atatttcacc atattttgca aaaggaactg tttcttatga ggcactttgc
     2761 agtgccaaaa tgctcggatt ttcaaaaata attcttgtgg ggcaggattt agcttatgtc
     2821 aacaatcaat gctacagtaa agatgctgct tattcagagc tggtatatga tgttaatccc
     2881 gaaaccaaaa aagtccaaat caaaataaat gattatgaaa aatatatcca atcgcttata
     2941 cccgagagcg gtgaaaaccc caatgttgat tacagagggt ttgcaagtta caaaataact
     3001 aatcttaatg atacattgta tttcgttaaa ggtgtaaaag gtgacatgct tcctacacaa
     3061 ggcggttatg ctacttttat agagcatttt aaggacttcg cctctaaaaa tcctgatttg
     3121 gatttaatta attcttcaat gataggagca aacatagaag gttttaaaaa tatttcgctt
     3181 gaagattcaa taaaagaaac cccttttata cttgaaaaac caaatctgtc aaaacaaaat
     3241 cgctcttatg acaaacaaag cattttttct tcacttgaaa gagaagaagt tcttttaaaa
     3301 gaaatcttaa aagagtttga acaggcaaga gaatatatat ttaaatacga ccgagaattc
     3361 aggagaagac gaaccctcag cgaagaaaca aacaagtacc ttaaattatt gcttaatctg
     3421 tacaacaaaa ttttattgga atatcaggga aacaacattt tgtatcaggc aatttctttt
     3481 aacgaaaatc ttgaagtcca atttgcgctt aaaagttctg aaaacgtaaa cattgttgaa
     3541 attcaaaatg tatatgaaca gctgaaaata tatttttaca tggttgaaga aaaattgacc
     3601 aaaacgatag aaaaaattca acaacagaaa gggaaagtgc ttgaaagcat taattcaacg
     3661 ggttaaaaaa gcttcggtaa aaatagacgg aaaactttat tcgcaaataa aatcaggttt
     3721 gcttgtgttt ttaggtgttt caaaatcaga taccaaagaa aacgctcaaa aattagccca
     3781 aaaagttttg agtttaagga tctttgaaga cgaacatggc aaaatgaata aatctgtcct
     3841 tgacgttcaa ggtgaaattc ttattgtatc gcaatttacc cttttgggag attgtaaaaa
     3901 aggcactcgt ccgagttttg atgatgctgc atctcctgaa aatgcaaaaa aactttatga
     3961 agaatttgtc aaaataatat cagattcagg gactagagta aaaacgggta aatttcaggc
     4021 aatgatggat gtttcattaa taaatgacgg tcctgtaact tttatgattg aaaaataaat
     4081 gtacaaaaca gggtattcta tttttaaaac ttttgtgtta taatcttaaa gtaatttata
     4141 tacccacttg ccaatatttg attttagtta taatatatgg aaaaaagagt acaaccacaa
     4201 cgaatttaaa ttgaacgagg agctaaatct atcagttttt tgttaattaa gaggctttca
     4261 ttatgtatgt aaacaaatgc accaagtgtg gggctgagtt tgaaaccaaa aaccccaaac
     4321 gagttatctg ccctgattgt ctgtatcccg ataaaaaaat gatgttatca tcagaaatag
     4381 agcaggaaga agttattgaa aaaccccaag aggttgacca aggcaataat cagcatgaag
     4441 aaccgcaaaa acaaaagttc tacagcagtt attcctatga aacgccccag cagcaacatc
     4501 cgcccagaca gaataattat ctcagaagag acaattactc agacagagga aattatcaaa
     4561 gaagagataa ttacggtgac aacagaggcg gataccaaag acctcagcag ggcgggtatc
     4621 aaagacctcg ccaagacggc tatcaaagac cgcaacaggg cggttatcaa agaagagata
     4681 attacggtga caacagaggc ggataccaaa gacctcagca gggcgggtat caaagacccc
     4741 aacaaggcgg atataacagg cctcaaggcg gataccaaag acctcagcag ggcggatatc
     4801 aaagacccca aggaggagct cctcgctttg gcggaaacag accaagaccg ccgattaata
     4861 aaaacaaacc tcttttagtt aataaggagc agctggaaca aatagaattg ctgtataaag
     4921 cgatgttacc gcttcctaat cctgatgcgc atgaagtaat cggagctaaa atcgaattag
     4981 aaccaagaaa agttttcttc ggcattaacc ttataagaca aaagcttatg cttccgaaat
     5041 tgcctttccc gaaaagaaaa ttggctattt cacccgatca gatgtttgca atccaaaatt
     5101 tatacgaacc gcttttacct cttccgccta tcggatgcca taaaatcata gcagctcagc
     5161 ttaaaatgga tgaatggcgc gttcacgtag gaatagggct tataagggca aaattggggc
     5221 tttccagatg gaatcaggaa agagcagatg cgcctgaaga atttaaaaaa gccgcaaaag
     5281 aaaaagtttc aaaagccaaa aaaaccaaag aagaaaaacc cgaaaaaaaa gcaaaagagc
     5341 ctaaagcaaa aaaagaaaca aaagcaaaaa aagaaagcaa aaaataggtt ttccgattat
     5401 ggataaatac gtttctgtag gcaaaatcct taattttcac ggaatacaag gcgaagcaaa
     5461 ggtcggtttt tcaaaaaatc aagccgattt tttgaaaagc ttaaagtctg cgtttataaa
     5521 attcgaaaac gagtacaaac ttttcaaaat ttcttcagtg aggtttaata aaaattttgc
     5581 tctgataaaa tttcagggca taaattcaat aaatgaactt ataccttaca aaaattgtct
     5641 tatttttgtt gaaaggcaaa cggcaagaga aaaactcgaa gaaaacgagt ttttgataga
     5701 tgaacttaca gggcttgatg tttttgacca aaataacgag aatataggta ttgcggtggg
     5761 tgtttcaaac aacggcataa atgattttat atcggtcaaa agcaaatcca aaaacatctg
     5821 tcttgttcct tttgtaaaag cccttgttcc cgaagttgac ataaaaaaca aaaaaattat
     5881 cgtaaataat attaaagggt tgattgaatg aaatttgatg tccttacgct atttccagaa
     5941 atgattgaaa actgttgctc acacagtatc ttaaaacgcg cttgccaaaa taaagttata
     6001 aaaataaaca caataaaccc cagagatttt acccacgaca aacacaaaaa agttgacgat
     6061 aaaccatttg gcggcggtgc gggaatggtt ttgatggcac agccttttgt ggatgcttac
     6121 gaaagtattg aaaaagaaaa aaattcaata accgtcatgc taacccctca ggggaaagtc
     6181 tttgacgaca aaatgagcaa tgaatttgca aaatatgacc agataatttt aatctgcgga
     6241 cattatgaag gatttgacga aaggataaga gaaataatta aaccgcaaga aatttccata
     6301 ggcgactttg ttttgacagg aggcgaactg cccgcacttt gccttataga cagcattagc
     6361 cgaaaaatag aaggaacact tggcaaacag gaatccgcag agtttgacag tttttcaaac
     6421 gggcttttgg aataccctca atacacaaag cccagaaact tcaaaggatt ggaagttcct
     6481 gaagttttac taaacggaaa tcattcccaa atagaaaaat ttcgtcacga acaaagcatt
     6541 aaaagaacaa aggcaaaaag aaaagatttg tgggataaat attctaacta acttctgata
     6601 acaggaagtc cgttgaattc aaaaattgac tctgaagttt ttctttcact cattcttttg
     6661 gcaagaattg cacccacaaa agcttctaac tgtgccatag gcatcatatt tggcggaatt
     6721 tcatcaaatc cgtacttggt tttaagtgca ttttgaagcg atttacaatc agctggagtt
     6781 ctttctttaa aacctgattc caaccctaaa gcctgtcttg ttaaataaag ttcaaaacgg
     6841 cttattttta tacctttttc caataatatt ttaaaatagt cagaacctct gtttgaatat
     6901 ctttgagtcc aattatccgt atttataagt ttttcacctg aatttaaaag ctgataaggt
     6961 tttgaaagaa ttctccactt cccgtttaac atggaattat cccaaggaag agaaacacaa
     7021 atataagcat ttttcaaaga attaaaattg tcaaaaaaga actgtatatc ctgcattgaa
     7081 aacagtttat ccaaagtaat aattgaattg tttttatcaa ttaccacaac acccgattcc
     7141 gtatttgccg aagcggcaag agataatcct ataaaatatt gttccattta ccttcctaca
     7201 ttcttaaatt cattttctac ataccttggc aaagcggttt ttccgccttg tgactgtgcc
     7261 ggttttgccg gaggcttaag ccatgaaccc tgccctgtta ttttagtctt tgtcgggtcg
     7321 tccaacactt gagtttcttt tgatattctc tttaactctt tttcataagt cgcatttaaa
     7381 atagccgctt gttctcttgc tcttttatcc gcatcaaaag atttatctga agtatttgta
     7441 attacattaa ttgtgtttat cgtttccatt cttctttttt gaagttcttc ctgttctttt
     7501 ttgctcattt tcataatatt gttttgagga acttccattg ctttaaattg attttttaga
     7561 attaaaagct caacccgtct gtttttgaac tgcccttgcg gagtacgatt tgaggcaatg
     7621 ggtcttgtgt ccgcaaaccc tattgcgcta aacaatccgg gcataaaacc gaaacgctct
     7681 attaaatatc ttatgataga gcacgaacgg gctgacgata gttcccaatt tgaaggaaaa
     7741 actatagaaa ctattttttg attatcggta tgcccttcca ctcttatgtt gtgaagaaaa
     7801 aaccttttac cgataacagc acctacttta tcaagttttt gttttgcctg aggtgttaaa
     7861 gttgcagaag cgggcttaaa aaggtaatta tcgttaaatc ttatcaaaag gccgcggtct
     7921 gtcatggttg cttctacacc gtcaatttca ccatttttat tcatgtcatc tatggttttt
     7981 tgaatatcat caaaagactg ttcttcatat ttttgactca tatattccat catcaatgga
     8041 gtaataagag tttcgccttc ggatttgtca aaaatttcct gacttttgtc ttgcataata
     8101 ttatctgcgc cttgcattac agaaggtgca gaaaaagctt gtttaataga ctcttccaat
     8161 tttttgaaat cagcaacgtc cacttgcgaa agtgcataca aaacaacaaa agtcgcaaaa
     8221 agcagagtta tgaagtctgc gtaagaaatc aaccaccttt caagattttc atgttcggga
     8281 tgtttttgtt ttctggccat tttaactacc ttgattgttc aaaaggaatg attggagttt
     8341 tctttcaatc aaggctgggc tttctcctgc ctgaatagct aaaactccct caagcattat
     8401 ttcttttgca acaaatactt tttgataatt aaattttatt ctggtagaac atggtatcaa
     8461 aacaaggttt gcggcaaaaa gaccgtaaat tgtagcaaca aaagcaactg caattcctgc
     8521 tcctaatttt gaaggatcgg agagattttg cattacttgg attaatccca taaccgcacc
     8581 tataatcccc aaagtaggag aatatccgcc ggctgattcc caaacttttg aacctttatt
     8641 aactttttcc tcgagctgag tgatttgatt ttccaacatt ccctgaacaa gcgaagggtc
     8701 tgcgccgtca gatacggctt ccaagcccag ggtcaaaagc ggatcggatg caaattttgc
     8761 ttctttttcc aaaactataa ccccttcttt tcttgccttt gttgcaaatc cgacaatttc
     8821 gcttataaga ttttcaaaat tgacggccgg cgggaaaaaa acatctttca gcataagaac
     8881 accgtttttt aaatccggca caggaaaact tgctataaca gcacctgccg ttccgccaaa
     8941 aacaatcatg ccggcagtta cttgaagtaa ttgacctata ttcccgcctt caagtgcctg
     9001 tgccgcaaga ataaagccca gtccaacaac tactccgact atagcgaata tgtccatgct
     9061 ttgctccgat tatagtttct ctaatataat ttaacaataa ttttaataat aataaatcgt
     9121 ataagtgaag taattagtga gaagtgaatg aggaggcagg agtgcgagaa ggcaagcggg
     9181 caagaaaaaa cttttcaact tatcgactgt tcgactattc aacttttaca atgttgtcaa
     9241 aataacaggt ttatcatctg taaccaaaat cgaatgctca aagtgtgcag aaggcatttt
     9301 gtcaacggtt tgtactgtcc atttatcggc taaaacttca accgcatcgc cgcccaaatt
     9361 caacataggc tctattgcta ttgtcattcc tgatttcaaa actgttttgt cgcaggttct
     9421 gtaattaaaa acaaaaggct cttcatgcat tgaacgaccc acaccgtgtc cgccatactg
     9481 ccttacaatc cctaattttt ctctttttgc aatatcttct atagcaccgc ctacattatc
     9541 caaaatattc ccgtgaaaca tctgagaaat cgccgcaaaa agtgcttctt cggttgcttt
     9601 caagagtctt tccttttcag aagaaatttt gcccacagga taagtccagg cactgtcacc
     9661 taccaatccg ccgtaagttg caccgacatc aacgcttatt atatcgcctt ctttaagaat
     9721 aactgattca agaggaaatc catgtacaac ctgttcattc aaagatgcac aaatacttgc
     9781 ggggaatccg tgatatccca aaaaagtcgg cacagcttta ttttttttga ttatgtcaaa
     9841 ggcaacatta tcaagttctt ttgcacttat cccaggctca atcaatcgct tcatttcttg
     9901 atgcacaagc gcgacaatat aaccagcgtg tcttattctt tttatttctt cttttgattt
     9961 tcttattatc attttaaatc ttttccacta aaattgtatc aaatattgaa ggttaataca
    10021 atattaaatt ttgtaacaac atgaaaaatt ttagcaaatt ttttttttac actatttatt
    10081 aaaagtgtag ctgacatata aaaaggagaa aatatgcaag tacaaagagt aaatatgaac
    10141 tcacaaagct ttggtgcaat tagatttgtt ccaagagatt taaaaaaagc tttaaaaatg
    10201 attgataact cggattttca aacattaatc aaaatgacta agtcaaataa caaagctgaa
    10261 tctaatgctg caaatatggt attcgattgg gttaatggta ttgaaaaact taaaaaaaat
    10321 ggttatgaaa ttacagaaga actcatagac tatactcgta aaatggctat taagcgttta
    10381 ggcaaaaacg gcaccgatgg cataacagcc ggcctttata actatacttt aggcagactt
    10441 gacgggtgct ggcttaattc aagcatatta aaggaattta ggaaaaaagg tcaattccta
    10501 tggccgcctg caaagtaaga taaacttttt tataaacaaa aaagcgggac aaaatgaaat
    10561 cattttgtcc cgctttttaa atatttaaac tatatagcac ttaaaagtct gtcccaaacc
    10621 tcatcaacac ttccgtttgc atcaattttt ttcaaagagc ctttttgctc gtaaaattta
    10681 atcaacggag ccgtttgttc aaaataggta tcaaatcttg attttgcgat ttcttcggtg
    10741 tcatcttttc tttggattaa ttcacatccg cactcgtcgc aaacaccgtc ttttcttggt
    10801 ttaagtgaca ataaattata aattttaccg cattttgcac atgaacggcg ataaattatt
    10861 ctctccataa gaagtttttg gtcaatatca aaataaatta ctttaaaatc aacttcttcg
    10921 cccgcatcaa tttctttaag catttcttcc aaaagaattg cctgctcggt gcttcttggg
    10981 taaccgtcta aaataaatcc gcttttgcag tctgattctt tcaatctgct ttttataatt
    11041 gaagctacga tatcaaccgg tacaagctga cctttttcga cataagattg ggctataagc
    11101 ccgtctgaag ttttatccgc cattgctttt ctaagcagtg agcctgtatc aacgtggggg
    11161 aaatttaaat gcaaggcaag tttgcttgtc tgagtgcctt ttccgcatgc cggcggtcct
    11221 aaaaatacta attctttttt cattgtcaat cctttttatt ttttcatatg tatgttttaa
    11281 gtaaatagta aatagttaat agttaataga gactaaaagt ctatttacca tttaccattc
    11341 actaatctac gtatcaatat tcgtggcata aacagcgttt gcttcgataa aatcccttct
    11401 gggctccact ttatcgccca taagaatatc aaataactgg tctgccaaca tcgcatcttc
    11461 aatgcttact ttcaacaatg tccttgttgc aggatccatg gttgtttccc aaagctgttg
    11521 gggcatcatt tctcccaagc ctttgaaacg ctgaatttgc aatccttttt gacctctttc
    11581 ttcgacaatc ctttgaagtt ctgcaaaaga acttatttca atttgctccg aatcagtttc
    11641 caaaataagc ttttcttctt cttcaatcaa gaagtttcta attaaaggat aagaagtttt
    11701 taatctctta tactctgaac ttttaataat gttagatgtt attaaaactg cctcaacatc
    11761 ttgtcctaaa ttaagcacaa gattgtattt tgcattttcc ggattgtatt tcaaagaaac
    11821 attgtatttt tctgcttctt taacattgta atttcttgca tggtcttgca aatacctgtt
    11881 caaataatca ataacttcat ccatttttga ctgattatca aagtcttctg cttcaagccc
    11941 cgagcgcacc aatcctctta aaacaaccgc aggaatagca tttaagatag ggttgttgta
    12001 agaattgtaa tatgtggaca tgtttgccat aaggtcggtg aaatctttgt cagaacacat
    12061 tttttgttta gatttgtcgc acaggcaaag ccctttaact ccgcgttctc tgagcatttt
    12121 atcaagtgct ctttcatcgt ataaatattc tgaagtttta cccgttgtaa ctttgaataa
    12181 tggtggttgg gctatataaa tatgcccgtt atcaattaat ggttttgcat aacggaagaa
    12241 aaatgtcaaa agaagcgttc tgatgtgagc cccatcaaca tctgcatccg tcatgataat
    12301 aattttatga taccttaatt tttcaatttc tacttcgtct tcgtttttgc ttatgttaat
    12361 cccaaaagcc tgcaccattg cctgaatttc attatttccg taaattttat caagccttgc
    12421 acgctcaaca ttcaatattt tacctcttaa aggaagaatc gcctgaaaca ttctgtttct
    12481 gccctgttta gcagagccgc ccgctgaatc tccctcaaca atgtagattt cgcatttttc
    12541 gggttctctg ttggaacaat cggcaagttt tccgggcaaa gtagagtttt ctaaagcgga
    12601 ttttcttctg gtcagttccc ttgcttttct tgcggcttct cttgcacgct gtgcctgaag
    12661 ggttttttca atgattattt ttgcgttttt aggattaaat tcaagccatt cctgaagctt
    12721 atctctgact acatcttgca ctgcacccat agcttcctca ttgccgagtt tttcttttgt
    12781 ctgtccttca aactcagggt tttcaagttt tacactgaca atagcagtca aaccttctct
    12841 tacatcttct ccggtcaaat tttgctcaga gtctttaagg atattatttt tccttgcata
    12901 atcgtttaaa acacgtgtta ttgcgtttct gaatcctgta agatgagttc cgccatgatg
    12961 agtattaatg ttatttgcaa aagacaaaac gctttcgttg taagcatctg tgtattgcat
    13021 tgcaacttct acaaggattc cttcaaccgt tttttccatg taaacagggg cttcaaaaag
    13081 cacagtttta tttttgttta aaaattcaac ataacttcct atgccacctt catagtggaa
    13141 tgcttcttct tcaagcgttt ttccttcaat aaaaactatt ttaagccctt tgttcaagaa
    13201 agccatttct ctgagcctgt ttgcaatggt ttctttatct atttctgttg tttcggtaaa
    13261 aatctcggga tcaggccaaa aagttgtctt tgtacctgtt tttgaagttt cttttgtttt
    13321 ttcaacagga gaagaaggtt cgccccttaa atattcctgc ctccaaacaa atccgtcgcg
    13381 agaaacttct acaatcattt tttctgaaag cgcgtttaca acggaagctc caacaccgtg
    13441 caagcctcct gaaactttgt atcctccgtc gccgaatttt ccgccggcat gcaataccgt
    13501 atgaactatt tctaaagctg attttccaga atcaggtttg atgtcaacag gaatacctct
    13561 gccgttatct tcaactgtta tactgtggtc ggggtttacc ataactttta tttcgctgca
    13621 ataacccgcc aaagattcgt ctatggaatt atccacaatc tcataaatgc agtggtgcaa
    13681 acctctttga gaagttgaac ctatatacat ccccggacga agccttacag cttctaatcc
    13741 ttctaaaacc cttatattac tggcatcata attgctatgt tctgtctgca ttgtcatttt
    13801 atctccccaa taagcgaaag gtgaagtatg aagtatgagg aggctgtgct tttctcttca
    13861 cccttcacgc ctcactcttc acttataata gcataaacaa aagattttgg cacgtttttt
    13921 gttaatattg cgtaaaattc gtaagtaaaa atacggactt aaaaaaatgt gccaaaaagt
    13981 ttataattaa gtatcaaacg ctttattttt ataacttgcc acagaaacaa tctcggaaag
    14041 gaaaattcaa tgagcaaagt ttcatcaata gattaccccg catttacaaa cggacaggtt
    14101 tcaataaacg gcaatcctgt cgccagtgcc acaatgtcaa acggggtaat taattcaaat
    14161 tacaatatga cagatgccga aaattacatt tataattacg ctcaaaatgc tcttgcaagt
    14221 attttgcctc agcttaatgt tttttcgccg gacactgtca attcgcttca atcacaaatc
    14281 gatgcctaca caaatcaagg ggtaaatacc ataaacaata tctattcacc catgattaaa
    14341 aatcttcaaa acgatatagc atcaagattc ggtaattttg acaattcaat ctttatggat
    14401 aacttaaaat caatcgaatc caaaagaagc gatgcggtaa gctcttttgc ccaagacatt
    14461 atggcaaaaa gaaacgagct tataaacaat gaactttcta accgctacaa ctatgcagac
    14521 tttttaaacc aaatccaaaa ccaaacgatt aataacattt taagctacat aaatacagct
    14581 ttaggaagtt cttcaagtgc caattcttac aataataatt tgtataatgc tttgtacaaa
    14641 caggctttgg caggtcaaaa ttcaacaccg aatttaagca atttgagttc ataccttgca
    14701 aacagcttag ggataaattc atttttgtaa tttgaaatac cttcaaaagc gcggaatttt
    14761 taaaaaaatt ccgcgctttt tattcaattc tatttactat ttactattta ccatttacta
    14821 tataattata ataaatattg gaaggtgcaa tttgaatata ttacaagagt ttgacacaat
    14881 agctgcaata gcaaccccaa taggcacagg cggagtcggg gttataagaa tctccggggg
    14941 taaatgtgac ttggattctt gtgataaccc ctcgtctttt gtgggtgaaa gtaaatgttt
    15001 tgaaattgtt gaaaaaattt tctccggcaa aaagcttatt gctggaaaaa ttcatcacgg
    15061 ttggattctc gataacgaca aaagaataga cgaagtgatt gtgcttcctt ttaaagcccc
    15121 aaacagctac acgggagaag atgtggtcga aattcaatgc catggcggaa taaatgttgt
    15181 aagaaacatt ttggatttgg tcttaaagca aggtgcaagg cctgcccaaa ggggcgagtt
    15241 tacaaaaaga gcctttctta atcataaact tgatttatca caagcagagg cggtttgcga
    15301 tttaatccat gcaaaaaccc aaaattttgc aatacattcg gctaaaaatt tgtcgggggt
    15361 cttaacccaa aaaatcggcg aaattaaaaa agatattttt gaggtcttat caaaaatcct
    15421 tgcaggaatt gattttcccg atgatgtcaa agagcctgaa tacgaatatt tacaaaaaga
    15481 atttcaaaat gcacttgaca aaatcgaatc gattttgtca agtgccaaat cttcaaacgt
    15541 attaagacag ggaataaaaa ttgctatttt gggcaaacca aacgtgggca aatcttctct
    15601 ttttaatgcg cttttgagca tggagcgggc gattgtaact gatattgcag gaactacaag
    15661 agatatttta accgaaacaa tcgacataga aggaattcct gtcactttaa tcgacacggc
    15721 aggcataaga gaacattcaa cagcagataa agtagaaaaa ataggaatag aattttcaaa
    15781 acaatcggca gatgaagccg acttgatttt gtttttatac gatgcttcaa caggaatgaa
    15841 caaagatgat gaagctattt tggatttaat taaagaaaaa aatcatataa aaatagcaaa
    15901 taaagcggat ttatatactg acaattaccc ctcatctgtc ctttggacat cttctcccgc
    15961 aaggggggaa gaaattatac cctcgcccct tgcgggagag ggaaattttt caatgcgaga
    16021 tacgagcatt agaaaaatgg gtgaggggtc atcaatctat ctttcaacca ttactcaaca
    16081 aggatttgac caattaaagc aaaaaataaa acaatttgcg ggtaagtgtt caagcgaaga
    16141 gcttgaattt accaccaaca ctcgccagca ggactgtctt gagcgggcaa aagaagcctt
    16201 aaccaacgcc cttgcagcgg cacaaaaaga ggaacttcag gatttgattt caatcgatgt
    16261 aaaatcagcc cttttaagtc ttgatgagat aacaggcgaa gtaataacag atgatatctt
    16321 aaacaacatt tttgaaaatt tttgtattgg aaaataatcc attatcttta tcttgtattt
    16381 tcaaacatgt cttgtatcca atttaaaaat ccggtaaaaa aagaaggttt tttataatta
    16441 acttttattg catcaagttc ttctattgtt ttttggcctg ctgttcttac tctgaatgaa
    16501 gatttttcaa caacatctct attgctaaag ttcttgttta aatattttac aaatttgccg
    16561 actcttttgt ataattttgc aaaaggaaga ttctcgggat tttcaatatc aaaaagttct
    16621 cccgtttttg aattcaaagt caatgcaaat tcatctgtag aaattttcac cctaggctca
    16681 aaccctggta aagcaccttc gtttataaat ttacctttta aagaatttaa tcccgtatcc
    16741 cattttaaag aaaaaccaaa gtctttgctg ccgacttttt tagtcccttt tcctagctta
    16801 tttaatagct tgcgtacttc caaaattgta gattcaggta accttcttcc ctgaaagctt
    16861 attttattta cttgcataaa tttactcctt ataattagtt acttttaatt aaagcgcgta
    16921 aaaaaatttt ttacgcgctt taattaattt atttacattt tgcaatattt aactactgag
    16981 gcttatgttt cttgtttttt ttaggcgcaa cccaagcctg ttcttctttt tctgggtttt
    17041 catttgcctg acactcgcaa ggaccctcta ttaagcaagc acagccttct tcgacttctt
    17101 cttctgagtc tttttcttcc tcttcagaaa tttcttcttt gggttcttct tcagaatctt
    17161 cgtctttatt ttcttttgtt tcttcttcct cttcttgtgc cttttcagcc tcggcttttg
    17221 ctttgcgttc ttcttcctct tttataaggg cttgaatttc ttcttcatct ttggctctta
    17281 ctacaggaat tgaaagcata agagccattg aatcgccttc ctgagcaaaa ttcaattcaa
    17341 gtgcttcttt gtccatttca acgtattttg aaagcagatc aatcatctca cctctcattc
    17401 tttcaagaag ttgaggagtt aagtttgttc tgtcttgcat tagtacaagc ttaagcctgt
    17461 tgcatgcgac ttcttttaaa ttttcttttt cgggcgtctg gaaaaatccc gccagttttt
    17521 tatatagttc agtaattatt tctctcatcg ctattactcc ttaccaaaca ggccggcaaa
    17581 gaactttttt acttttgctg cccatccttt cggctcctcg agattaagaa aatcaacctc
    17641 ttctcccatt actctttttg caacatttga ataagcctga cccgcaagtg atttttcgtc
    17701 atttacgata ggttcgccct tgtttgttga agtaataacg cccgtgtctt cgggaataat
    17761 tccgattaaa gaacaagata aaatatccac aacatcttca acgctcatca tatcatttga
    17821 tttgataagg ttaggtctga ctctgttcaa aagcagcctg taagattcga tttcttcttt
    17881 tgcttccaaa agcccgataa ttctgtcggc atctcttacc gcagacattt caggagttgt
    17941 aacaactatt gcttctgtag ctccagcaac ggcattttga aatccttgtt caattcctgc
    18001 aggacaatca acaagtataa aatcgttatc ctctttaagt tcatcgcaaa tcgctttaag
    18061 ctgttcttga ttaaccgcac ttttatcgcg ggtttgtgcc gctgctaaaa gacaaaggtt
    18121 aggatttttc ttgtctttca caagtgcttg cttaagcttg caacgctctt ccacaacatc
    18181 gactattgta tagacaattc tgttttcaag ccccaaaagc aagtctaaat ttctcaaacc
    18241 gatatcagta tcgattaaaa caactttttg ccccgctttt gcaagagctg taccaatgtt
    18301 tgcgctggta gttgttttac ccaccccgcc ttttcctgaa gtaattacta ttactcgacc
    18361 gctcatttat atctcctttg tttattagta aatggtaaat ggtaaatggt gaatagaaat
    18421 tttaaaaatc tatttactat ttactattta ctattcactc gctatataac ttataaatta
    18481 ttatttcacc atgttcattc acttttgcct cttcaggtgt aaactggctt gtcttttgga
    18541 taaacggaat gttcaaagaa tccgggcgtc ttgcgtaaaa cccggaaatt ctaagctgaa
    18601 ttgcgtttat cttaagggcg cgaatttttg cccaatcatt tcctcgagct cctgcatggg
    18661 caattccacc aagtattccc caaacagtaa tgtcaccttt tgcaataatt tcgcttcccg
    18721 ggtgagcatc gcctataaga acaatgtttc cgtcatagct tatggtttgc cctgaacgca
    18781 aagtctgttg caggtacaaa gttgacattt ttgaaacttc atgttcggat tcttcttttt
    18841 gcttatcctg ctcattttct tcgtcaatca gttcttccaa tttatcttct gcctgaagtt
    18901 cttcgtctgt tttttctata acctcaattt tttcttcaac ttgttcgggc tcaatttttt
    18961 catacaaatt ctctgaaaca gcttcatgcg aattgtcttc aaaaagataa tcctgtacct
    19021 gctcatcttc aacaacttcg atttcgtttg aaagttttga aacaattaac cctaaattca
    19081 aggcagcaag ctctgtttga tttgaattcg tgtcaacaaa agcaagctcc gagttaagcg
    19141 aagtaatcaa agattttatg ctcaaaagct gagattgagt taaatcaata ttaccaagct
    19201 tcaagcaaat ctgcctgtat tgggcttcgg gcatttcgag aattgaactc aattcataaa
    19261 taatatcgga cgcgtctttt gcgtttccca aatcgattat aaacccgttt tcaacaaaac
    19321 cttttgtcat atcacttcct caaatcttat ataaattaat cgtatctgaa agatttttgt
    19381 tttacaaata attgttgcaa tttggttact tttttatacg aaacttaatt ttcttaataa
    19441 atcttatttt aaatattttt tcattgacca aatttttaaa taatgttaaa atgaattgtt
    19501 taaaaactga gaaaataaat atgaccttaa acatatttaa aaattatccc aaaaataata
    19561 acaaaattaa aaccgttagt tttgcggctt ttcgcaatta tggatttact tgaaaaaccc
    19621 tgagacattg ttgacacatt tggtacaatt attaccaaag gagtc
//
LOCUS       DAOQAU010000032        19144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_2994_length_19144_cov_7.909634, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000032 DAOQAU010000000
VERSION     DAOQAU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 19144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19144
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2994_length_19144_cov_7.909634"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3295
                     /locus_tag="PKI94_05760"
     CDS             <1..3295
                     /locus_tag="PKI94_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW26281.1"
                     /translation="IDIALPIFVCLFILGLFLFVKDSLQIVSETNRNNFEYTVKIRAS
                     ISKIDKIIENSEVSLNIISDVIQRSYDINKLYDKNYNYEYIKQNDILTRCALKNSPGI
                     NGIWFQLNADVPFSSKIYNWHILKEGKIVNLRKFFEKESNNDRKINPEEDPYYFEAIK
                     AKKTIWSDLYTDSDIKTQMITISKPIYKKNKLIGVIGIDISVFDFEQALKNMKASFED
                     SEAFLLDNNNKIILSSDTKIPFKDFTFLKLNQYNSINKEYMAEYSDEGTDKTAIIIPL
                     SNKYKVIITFPHLSVFKGFDRLFRTVYFIFGVLIVLACMAFYNRQKIIKVNKQLENET
                     FKLKTIIDTAPTAIVVKNKEGVYIDCNNKLLEIMEVERQDFIGKTDYEIFSKETADIL
                     TEQDEIVKTTKQLKVSENWHTTKTGKSILLTKFRVPLFDINNQVIGILINAIDVTQKH
                     NEQEQLEIAKEAAENATKMKSNFLANMSHEIRTPLNGVLGFLQLLEDTDPTEEQKEFI
                     TDAQKSSDILLNIINEILDFSKIEAGKLKIDNISFDIRSLVEDITVINIPNACQKRVD
                     INSLICSDVPQRVFGDPGRVKQILNNLVGNAIKFTEKGEVAIYVNMESETDEEVFINF
                     KIKDTGIGIEKEKLEMIFETFTQADESTTRKYGGTGLGLAISQKLASLMGGGINVESV
                     LGEGSTFSVSIKFKKDKEAESEAHNLIKSIENETVLIVENKCTDLRIIKYYLNEANCF
                     VKEVRNSNEVLSLINDENQNISAIIIDYEMEASGEISLSNIIKSNEKSKDIPLILYTS
                     VAQKGDALSAKEKGFAGYISKPIKKQELIESIALAINNKGAQIKNELITKHLVKENKF
                     NLKTKILMVEDTELNCKFVTKVLNNSGLNCDVAYNGQEAIEAYKTKKYDLILMDCQMP
                     IMDGYEASEKIRELEVGTNSHIPIIALTANAMQHDREKCFRAGMDDYITKPVKTQELL
                     SLIGKYVNVEDTYSENFEQNVFVQDNIDKMVSEIGFSQEEAAQMYKEFLEFLSQTIQE
                     LEKATEDGDFEQLGKIAHKLKGASANLRFEKISQISARLEDEKLNQSREVCIDLITQI
                     KNESEILKS"
     assembly_gap    3557..3656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3695..3771)
                     /locus_tag="PKI94_05765"
     tRNA            complement(3695..3771)
                     /locus_tag="PKI94_05765"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3735..3737),aa:Asp,seq:gtc)
     assembly_gap    3817..3916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4171..4416)
                     /locus_tag="PKI94_05770"
     CDS             complement(4171..4416)
                     /locus_tag="PKI94_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26282.1"
                     /translation="MTTIIGVRLDNRTQTALDFQKVLTHFGCSIKTRLGLHEVAANVC
                     APNGLILLEVIHDDEAENLKKELKEIEGIEIQSMKFD"
     gene            complement(4552..4920)
                     /locus_tag="PKI94_05775"
     CDS             complement(4552..4920)
                     /locus_tag="PKI94_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26283.1"
                     /translation="MPQKKKLTKLTNKEVEMLNRFKNLKIVQKLKEVSPKLKWLMFNQ
                     MQDYKINSKYIDMICSDEAKEINSDEQRLEAMVRESLIKEFSPEIENMKSYEFLVDAV
                     VHNLKKKQLQATDGDQEIAG"
     gene            complement(5042..7051)
                     /gene="glyS"
                     /locus_tag="PKI94_05780"
     CDS             complement(5042..7051)
                     /gene="glyS"
                     /locus_tag="PKI94_05780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNW26284.1"
                     /translation="MSKYLLEIGTEELPYRFIPQAIEQLEEGFKKFFEENKVEYKNID
                     VLATPRRLAVIVEGLSKKQPDEEKIIKGPPKNTACDEKGNLTQAGKGFLNKNGIDEKD
                     AYIKDDYLHAKVFIKGKLTSELLEENIAQIVLKLQGPYFMRWGANDEKFSRPIRWIMS
                     ILDDKELPVEMVGVKSSKFTRGHRFAKEKTIEINNPDEYVEKLREAYVIVNQNERKDK
                     IENIVKNKCYRINPKLIEEVTYLCEWPVACECEFNPKYLDIPEMVTVTVMQCHQRYFP
                     IYKDSKLTNKFITITNYLGDDFSNIKAGNERVVTARLEDAIFFFKEDTKKPLAKYVED
                     LKGMTFQKGMGTMYDKAQRIVALSKEIASELKVNSPAIERTALLCKADLATSLVFEFT
                     ELQGFIGADYAKISGEDKKVCQGIKEHYYPLNAESELAQNIEGQVVGIADKLDTICAV
                     FVGGKIPTGSSDPLGVRRAALGIIKTIIDKKLDLNLSELIKQTLDLLPQKADILNKIE
                     EFFIQRLIVLLSDKYRKDILEACTKNKKSLVDLNDFIKRVELIENKINTKEFASIFES
                     ANRVSRILGANIIDKEPEISLFAYEAEKDLYNKIEGFDQQKITAKEYDYLFQKLIEIN
                     PSIEKFFEDVLVMDKDEKIKQNRFALLSKIKKLYDMISDFSYLQT"
     gene            7108..7311
                     /locus_tag="PKI94_05785"
     CDS             7108..7311
                     /locus_tag="PKI94_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26285.1"
                     /translation="MFKNRFYIVDIGRKTLHNNCMFSGGRFKIKDFAKISAFTLAEVL
                     IVIGIIGMIADLTLPNLIKDFEK"
     gene            7426..8178
                     /locus_tag="PKI94_05790"
     CDS             7426..8178
                     /locus_tag="PKI94_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26286.1"
                     /translation="MGGVNVTLPDGSLAYGPLNYPSQIFVNTYLRPYLNVAEDCSYTT
                     ARAANASWREKCGVYNTTYKNYPMKYLCAARNNACVFSNTIETNPQGGAYSRLLLKDG
                     TLLQMYIVNELRYEYSDTTTPGVCPSPAPACTKMAAVPDSYNDLGEIVPGTPERCRCH
                     TNEYRIRANIYVDLNGIKPPNVFGKDIFLLNLSPSNGTIVAYPGVGKTRTTLTTNTPA
                     DNAPCKSNRYGLYCGALIIYDGWKIAPDYPWP"
     gene            8645..9922
                     /locus_tag="PKI94_05795"
     CDS             8645..9922
                     /locus_tag="PKI94_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26287.1"
                     /translation="MTLGTTIYSILGNNASIIPLGIKDVSNALGQTAGSYLAGDKIEG
                     KDRFIDEFGTQAIWIGGIPLYKKLIDSTLYKLFGFNSKINVRVLKDPEILKKAAEQAP
                     EHLKAGFEKLLESGKNQKIFKGLFSARFIASTALTLASYNALTYFRHKSTEKSVLAEI
                     KREEELKKTSQKFIENKNKKSFDKFPVAFKGGFQDFMFNPVKNMMIIDAGISSQRLIS
                     SRNLQDFIGYSIKEGAFWFFMYFAGAKIQKIFENRAAAKHKPIDLDIRALQNKNLKTL
                     FENGTIESKLKEFSLEGSDAKIYESLFNNNNIVVETAKKSDIIKTMKKTGEINTGHYI
                     DIKEIKGVHTKLQNLLNGFKEFEAKGKGGVDKYFKEVTDLKRLSVLKNIGTCIGFLGI
                     IVPGTMLVVRLLRKDKEFQVKKEIKEQVLNKKG"
     gene            complement(10051..10488)
                     /gene="aroQ"
                     /locus_tag="PKI94_05800"
     CDS             complement(10051..10488)
                     /gene="aroQ"
                     /locus_tag="PKI94_05800"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNW26288.1"
                     /translation="MKILIINGPNLNMLGTREPEKYGFETLADIEKELYAYSFELSVD
                     IETFQSNIEGEIIDKIQKANFGFDGIVINPGGYSHTSVAIRDAISAVNLPCIEVHMTN
                     VHAREEFRHNSLIAPVCIGQITGFKKNVYKLGLSAICEYLNSK"
     gene            10555..11091
                     /locus_tag="PKI94_05805"
     CDS             10555..11091
                     /locus_tag="PKI94_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26289.1"
                     /translation="MKSGKKRLHLDGKKRVTLGKLISKDTTFFEVEKKSDGTLILKPQ
                     SALNNDEAWIYKDKKTFKNLKKSLLSLKKALITKIDNDFWDGMNELKDLKYTNEFLKN
                     LEPIISKNNSKLFEGIKGSLKKIISNNAQKLTLKNLKTHENKEVLLTSLEGKEADYKI
                     FWTTKNSQIIVINLIKKT"
     gene            11145..11480
                     /locus_tag="PKI94_05810"
     CDS             11145..11480
                     /locus_tag="PKI94_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26290.1"
                     /translation="MNQRRWYEKDPILKEALELLKLSCDETKTSAANFILQLQEQVAS
                     EVIEHVYDIMTRYEGGGKRWYDTDPVIMKAIELLRVAPKKTQRKAALKLLLALEDQSF
                     DNLDLKLEE"
     gene            11493..12296
                     /gene="folE2"
                     /locus_tag="PKI94_05815"
     CDS             11493..12296
                     /gene="folE2"
                     /locus_tag="PKI94_05815"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNW26291.1"
                     /translation="MNTNLKDVQNLKDTRGVDIQKVGIKHLELPLIIQRKNESNQVVS
                     AKAGVNVSLPRHYKGTHMSRFIEVLSEWKNKNLLGVDIKGCLHTIAKKLNAQSAELEF
                     NFKYFIDKKSPVSKILSPMSYDCSFEGKLINFGKKDEDYKFTLGVKVPVTTLCPCSKE
                     ISSQGAHNQRAIISVKISYDENSHIWIEDLIEMIEKCGSCPIYPLLKRADEKYVTEKA
                     YHNPKFVEDVLREAVLKLRENKIINSFEVECEAFESIHNHSAWAYQKEN"
     assembly_gap    12387..12486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12487..12869)
                     /locus_tag="PKI94_05820"
     CDS             complement(<12487..12869)
                     /locus_tag="PKI94_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26292.1"
                     /translation="MKNKLWLNLLFSFLFLMFSTALFACATEPNAPMSQNAPVTQTIP
                     AQSSAMPATIPEKNAYELTHMEKDGFKYGILKFLLAMTGVLVSALAIFLGLKIYQKFV
                     LNKTTMPAKTVNGSELETPKDFKDAL"
     gene            complement(12847..14532)
                     /locus_tag="PKI94_05825"
     CDS             complement(12847..14532)
                     /locus_tag="PKI94_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNW26293.1"
                     /translation="MENKRKKIIIAERDNIAVVMEGKKIMEFFVHRGDVLLGDIYLAK
                     VDNILPSIDAAFVDVGGDKMGFLHAEDVMGKGSLREKLSPKQKLVVQVVKEPTGHKGP
                     RVTTEVSLPGRFLVLIPNDTGVNVSKKIESSKERARLKALVSLLKPVGVGIIIRTEAE
                     GQSEADIQEDLEVLLEKWNNIVTSADTLTPPNLLYRDQDLLYRVIREACTEDISEIVV
                     DTSFAMNRVKHLLQNWHMNKNIQVTLYRGKDPLLTTMDIHKEIKASLQPKVNLPSGGY
                     LYVQTTEALTVIDVNSGKFISSSSQDETILKTNKEAIKEIARQLKLRNIGGMIIIDFI
                     DMTSRVDKLAILEELELALAPDKAKPQIGQLSDLGLVELTRHRQGQSLAELFAKKCPH
                     CQGNGYIMEELHFCAPIIESEFTQNQPQPKRRKQQGNFKKQFDRNQNNDQNQPQEEIL
                     PKESLVEQQAKEETPQKEEKRFNKNKNHRFNKNKKKDYRQPETTSIQENTSGHETHLQ
                     EVIKEVIQEVISPKPSKQKTKKTVEEKPVKSIKKRVPKKIQKQEVKHEEQVMA"
     gene            complement(14589..17186)
                     /locus_tag="PKI94_05830"
     CDS             complement(14589..17186)
                     /locus_tag="PKI94_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNW26294.1"
                     /translation="MKNFFEEYHKLLYNCNKPYQYVGGEFLSFNKNFDEAKIRFLFAF
                     PDKYEIGISNLGQRILYGLVNDNPDFMADRVYAPEADFKKALIKTKKELYSLEAKIPM
                     KNFDIIGFSLQYELSYPTVLAMLKMGKINIKSNQRSQDEPIIVAGGPCAYNPLPLADF
                     VDAFLIGDGEDLILEVCEVLQRGKAQGLCRAEKIAKLAQLEGVFVPQLYFGKTPHPNP
                     LPQGERGMNKSSNHLPQKIKKRIVQINYENALKTYPIPFSSCVHDRAIVEIRRGCGRM
                     CRFCQPGHVNLPVRERKAQDIIKIAKELVKNTGYDEYSLLSLSSNDYSNIKEVIKELC
                     VDFNEKKISVSLPSQRIDGFNLELANLVQSVRKSTMTLAPEAGSQRLRDVIRKNITEE
                     QILNAALTLYENGWSKIKFYFIVGLPTETYQDLDEMAALLEKIKDRAKLLKKEKELKH
                     SLDITCTLSIFVPKPFTPFQWAQQPSLDEISSRIYYLKDKTKHIKGLKINYHDKFTSQ
                     IEAVLTRGDKKLCAYIEELFKAGCYLDAWGEYFDKNIWLECAKKVEISLDELSQKEYE
                     LNQDLPWDFIDIGVEKDWLINEYKDALKSKTNPSCQTNCVNCGVCNNFKVKKVMDKKY
                     NAKIPQQAQDNKINCSTFQLFDFSTKYRIKLTKSGILRYFSHLDWQNTFHKALSRSGL
                     KVAYSQGFNPTMKVSLGVALPLFIESGCELVDIELLDDVCKEEVKLKLEKVLPEGCKI
                     KSIVKIDKSAQAIDNTVQWAQYEIKPFDNTLQNFESLRYNMDRVLSSEEILLTKKNKK
                     GLTKTTNIKSAIKSYEFIDDCLFIVLKTGQGTDVPALRADDLMKIIAPDILFDIKRVK
                     FFDRDMNEI"
     gene            complement(17307..17651)
                     /locus_tag="PKI94_05835"
     CDS             complement(17307..17651)
                     /locus_tag="PKI94_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26295.1"
                     /translation="MTDEINKKVISIFSKHKKDIPLKEEERVKFFAGFDYVRLDRDNN
                     GKKFNAEHLNKYAQKCHYIVRVMRNIDNEVLLYNYDISNDQLFEFIKAFENNTLNGTI
                     IEIDKYFPEDLA"
     gene            17850..18104
                     /locus_tag="PKI94_05840"
     CDS             17850..18104
                     /locus_tag="PKI94_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26296.1"
                     /translation="MLPIITEEHSSLAFAEIFQDVHSWRKKMIHYLKEENPEINTAII
                     EAANNTDLDPKAVALGAYMTYVLLEMAIKDDEIILGGEEE"
     gene            complement(18108..19019)
                     /gene="era"
                     /locus_tag="PKI94_05845"
     CDS             complement(18108..19019)
                     /gene="era"
                     /locus_tag="PKI94_05845"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNW26297.1"
                     /translation="MNKNTYKSGFVAIIGRPNVGKSTMLNQILGQKIVITTDKAQTTR
                     KRIKGILTTNEGQIVFIDTPGVHKPLNKLGEFLLDEAKIAIPDADLILFLVDGSEPSG
                     KGDKWIVENILNQSKTPVLLVLNKIDKTNPEKINANLETYKNLTIQPLSHSTIQLSAK
                     TGKGKEKLIYEILNLLPEGQKLYDEDVVTEETMRDVASEIIREKILINTKEEIPHSVA
                     VKIENYEEKPQIDRIFANIYCETPSQKGILIGKGGSLLKIIGTQARLELEQIVEKKVY
                     LELNVKVEKDWRKKEQMLKNFGYKSQK"
BASE COUNT         5998 a         3163 c         3350 g         6333 t
ORIGIN      
        1 tatagatatt gccttgccga tttttgtttg tttatttatt ttagggcttt ttctgtttgt
       61 taaagacagc ttgcaaatag tttcggaaac aaatagaaat aactttgaat acactgtaaa
      121 aataagagct tcaataagta aaatcgataa aataattgaa aattcagagg taagtttaaa
      181 tattatttca gatgttattc aaaggtctta tgatataaat aaactttatg acaaaaatta
      241 taattacgag tatataaaac aaaacgatat cttaacccgt tgtgcactaa aaaattcccc
      301 cggaataaac gggatttggt ttcaactaaa tgcagatgtt cctttttcat ctaaaattta
      361 taattggcac attttgaaag aaggtaaaat agtcaattta aggaaatttt ttgaaaaaga
      421 aagtaataat gacagaaaaa tcaacccaga agaagacccc tactactttg aagccataaa
      481 ggcaaaaaag accatttgga gcgatttata cactgattct gatataaaaa ctcaaatgat
      541 aacaatatca aaacctatat acaaaaagaa caagcttata ggtgttattg ggatagatat
      601 ttcagtattt gattttgagc aggcattaaa aaacatgaag gcttcgtttg aagattcaga
      661 agcatttttg ttggacaata ataataaaat aatactttct tctgatacaa aaatcccttt
      721 taaggatttt acttttttaa aattaaacca atataattct attaataaag agtatatggc
      781 agaatattct gatgaaggca ctgataaaac tgccattata attcctttat caaataaata
      841 taaagttatt ataacattcc ctcacttgtc tgttttcaag ggctttgacc gtctttttag
      901 gactgtttat tttatatttg gggtcttgat tgttttagcc tgcatggctt tttataacag
      961 acaaaaaatt ataaaagtca acaagcagct tgaaaacgaa acctttaagc taaaaacgat
     1021 tattgatacc gcacctactg ctattgttgt taaaaacaaa gaaggggttt atattgattg
     1081 taataataag cttcttgaaa ttatggaggt agaaaggcaa gattttatag gaaaaacaga
     1141 ttacgaaatc ttttctaaag aaacggcaga tattcttaca gaacaagacg agattgttaa
     1201 aacaacaaag caattaaaag ttagtgaaaa ttggcataca actaaaacag gaaaaagtat
     1261 tttacttaca aaattcagag ttccgctttt tgatattaat aatcaggtaa taggaatatt
     1321 gattaatgca atcgatgtaa cccaaaaaca caatgagcaa gaacagcttg aaattgctaa
     1381 agaagctgca gaaaacgcaa caaaaatgaa aagcaatttt ctggcaaata tgagtcatga
     1441 aataagaacc ccgcttaatg gcgtgttggg atttttgcaa ttacttgaag atacagatcc
     1501 tacagaagaa caaaaggagt ttattacaga tgctcaaaaa tcttctgata ttttgcttaa
     1561 tattattaac gaaattttag atttttcaaa aattgaagcc ggtaaattaa aaattgataa
     1621 tataagcttt gatatccgct ctctggttga agatattaca gtaataaata tacctaatgc
     1681 ttgccaaaaa agagttgata taaattcttt aatttgttct gatgtaccgc aaagagtttt
     1741 cggagacccg ggtagagtta aacaaatcct taataatctc gtgggtaacg ctataaaatt
     1801 taccgaaaaa ggagaggtgg ctatatatgt taatatggaa tctgagacag acgaagaagt
     1861 ttttatcaac tttaaaatta aagatacagg aatcggaatt gaaaaagaaa agcttgagat
     1921 gatttttgaa acgtttactc aggcagacga gtcaaccact agaaaatatg gcgggacagg
     1981 gcttggttta gctatttcac aaaaattagc cagtcttatg ggtggcggga taaatgttga
     2041 aagtgttctt ggtgaagggt ccacctttag tgtaagtatt aaatttaaaa aagataaaga
     2101 agctgaaagt gaggctcata atttaataaa atcgatagaa aacgagacgg ttttgatagt
     2161 tgaaaataaa tgtacggatt taagaataat taaatattat ttaaatgaag ctaactgttt
     2221 tgtaaaagaa gttcgcaata gcaatgaagt attaagctta ataaatgacg aaaatcaaaa
     2281 tatatcggcg ataataattg attatgaaat ggaagcctca ggcgaaattt ccttaagcaa
     2341 tataataaaa tcaaacgaaa aatcaaaaga tattccttta attttatata catcagttgc
     2401 tcaaaagggg gatgctctca gcgcaaaaga aaaaggattt gcaggatata tttcaaagcc
     2461 gataaaaaaa caggaactta ttgaaagcat tgctcttgca attaacaata aaggggcgca
     2521 aattaaaaat gaacttataa caaaacatct tgttaaagaa aataaattta atttaaagac
     2581 aaaaatactt atggttgaag acactgaatt aaactgcaag tttgtcacga aagttttgaa
     2641 taattcgggt ttaaactgtg acgttgctta taacggtcaa gaagcaatcg aggcttacaa
     2701 aacaaaaaaa tacgatttaa ttttgatgga ttgtcagatg ccgattatgg acggttacga
     2761 agcctcagaa aaaatcagag agctggaagt tggcacaaat tcgcatattc cgattatcgc
     2821 acttactgcc aatgcaatgc agcacgatcg tgaaaaatgc tttagagcag ggatggatga
     2881 ttatataacc aagcccgtaa aaacccagga attattatct ttaataggta aatatgtcaa
     2941 cgttgaagac acttattcag aaaattttga acaaaatgtt tttgtacaag acaacattga
     3001 caaaatggtt tcagaaatag ggtttagcca agaagaagcc gctcaaatgt ataaagagtt
     3061 tttagaattt ttgtcgcaaa ctattcaaga attagaaaaa gcaacagaag acggtgattt
     3121 tgaacaactg ggaaaaattg ctcacaagtt aaaaggggca agtgcaaatt taagatttga
     3181 aaaaatatct caaatcagcg cacggctaga agatgaaaag cttaatcaaa gcagggaagt
     3241 ttgtattgat ttgataactc aaattaaaaa tgaatcagaa atcttaaagt cttaataatt
     3301 taaatggttt atgagccgta caaatctgcg tgcgtgccaa gaaccggatt gcctttgcaa
     3361 tccgcgtgag agacccgtcg aaataaaaat accactcaaa tgagtggtat atatggcggg
     3421 gacgacgggg tgcgatgatt caagcaatct ttgattgcgt agaatcatgt atgcagccag
     3481 ctagaatttg taggcgaatt aatgagccgt acaaatctgc gtgcgtgcca agaaccggat
     3541 tgcctttgca atccgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgtc
     3661 gaaataaaaa taccactcaa atgagtggta tatatggcgg ggacgacggg gctcgaaccc
     3721 gcgacctcat gcgtgacagg catgcgctct aaccaactga gctacgcccc catattattt
     3781 gattttcatt tttggattgt agctcagttg gtttttnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnngctc agttggtttt ttaatttgac ttcgtttcgt caagcctcac
     3961 cgagactacg cccccatatt atttgatttt catttttgga ttgtagctca gttggttttt
     4021 taatttgact tcgtttcgtc aagcctcacc gagactacgc tcttatatta tttgactatt
     4081 ttaattatat ttgctgaagt tttttttgca agccgaaggc aaaataaagc gggcgcagca
     4141 agcttgctgc gcccgcttta ttttaaaaaa tcaatcaaat ttcatagatt gtatttctat
     4201 tccttcaatt tctttcagtt cttttttaag attttcagct tcatcatcgt ggattacttc
     4261 aagcaaaata agtccgtttg gcgcacaaac atttgcggca acttcgtgaa gccccagtct
     4321 tgtttttatt gaacatccga aatgtgttaa aactttttgg aagtccagtg cggtttgagt
     4381 tctgttgtca agtcttactc cgataattgt tgtcataatt tatgctcctt tcatattttt
     4441 aaataattta gcacaaaaaa aataatactc atactataca tacgagtatt attttttaaa
     4501 cctggtttta aacgcattgt gtacgtttaa aactgtcgga cgggaaagtc gctaccccgc
     4561 gatttcttga tctccgtcag tggcttgaag ctgttttttc tttaagttgt gcactactgc
     4621 atcaacaaga aactcataag atttcatatt ttcgatttca ggcgaaaatt cctttatgag
     4681 tgactctctg accattgctt ccagcctttg ttcgtcagaa tttatctctt ttgcttcatc
     4741 cgaacatatc atatcgatat atttggaatt gattttgtaa tcttgcattt ggttgaacat
     4801 cagccatttt aattttgggc tgacttcttt taatttttga acgattttta aatttttgaa
     4861 tcggttgagc atttcgacct ctttgttagt tagttttgtt agtttctttt tttggggcat
     4921 gttgtcatgt tcactaattt aaagtaccct caaaaaaaat atttaccttt ttggacagaa
     4981 agtttacaaa acttaaatta ttttttaaaa accctcgttt agagcatatt agcacatttg
     5041 attatgtttg taaatagcta aaatccgata tcatatcata taatttttta atttttgaaa
     5101 gtaatgcaaa tctgttttgc ttaatttttt cgtctttgtc catgaccaaa acatcttcaa
     5161 agaacttttc tattgaagga ttaatttcaa ttaatttttg aaaaagataa tcatattctt
     5221 tggccgtaat tttttgttgg tcaaaccctt caattttgtt gtataaatcc ttttctgcct
     5281 cataagcaaa aagcgaaatt tcaggctctt tgtcaataat atttgctccc aaaattcttg
     5341 aaaccctgtt tgcactttca aaaattgacg caaattcttt tgtatttatt ttattttcaa
     5401 tcaattcaac tcttttgata aaatcattta aatcaacaag agattttttg ttttttgtgc
     5461 atgcctctaa aatatcttta cggtatttat cagagagtaa aacaatcagc ctttggataa
     5521 aaaattcttc aattttattt aagatatcgg ctttttgagg caataaatca agagtttgtt
     5581 tgattaattc agacaaattt aaatccaatt ttttatcaat tattgttttt ataattccaa
     5641 gagccgcacg tcttacaccg agtggatcgg atgaacctgt ggggattttg ccaccgacaa
     5701 aaaccgcaca aatcgtatct aatttatctg caataccgac aacctgccct tcgatatttt
     5761 gggcaagttc gctttcggca ttgagcgggt agtaatgttc ttttataccc tggcaaactt
     5821 ttttgtcttc gcctgaaatc tttgcataat ccgctccgat aaagccttga agttcggtga
     5881 attcaaacac aagacttgtg gcaaggtctg ccttgcaaag caaagcggtt ctttctattg
     5941 cgggcgaatt tactttcagc tcagatgcta tttcttttga aagcgcaaca attctttggg
     6001 ctttatcata cattgttccc atgccttttt ggaaagtcat tcccttaagg tcttctacat
     6061 attttgccaa aggttttttg gtgtcttcct taaagaaaaa tattgcatct tcaagtcttg
     6121 cggttaccac tctttcgttg cccgctttaa tatttgaaaa atcatcgccg agataatttg
     6181 ttatggtgat aaatttgttt gttaatttac tgtctttata aatcgggaaa tacctttgat
     6241 ggcattgcat aactgttact gtaaccattt cgggtatatc cagatatttt ggattgaact
     6301 cacattcaca agcgacaggc cattcacaca aataagtaac ttcttcgatt aatttcggat
     6361 ttattctgta acatttattt ttgacgatat tttcaatttt atcttttcgc tcgttctgat
     6421 taacaataac gtatgcctct cttaattttt caacatattc gtcggggtta tttatttcga
     6481 tggttttttc ttttgcaaat ctgtgtccgc gtgtgaattt tgatgattta actcccacca
     6541 tttcaacggg aagttcttta tcatctaaaa ttgacattat ccatctaata ggacgagaaa
     6601 atttttcgtc atttgctccc catctcataa aatatggtcc ttgaagcttc aaaacaattt
     6661 gtgcaatatt ttcttccaat agttctgacg ttaatttgcc cttgataaaa actttggcat
     6721 gcaggtaatc atctttaata taagcgtctt tttcatctat accgttttta tttaaaaatc
     6781 cttttcctgc ttgggttaag tttccttttt catcacaagc agtatttttt ggaggtcctt
     6841 tgataatttt ttcttcgtcg ggctgttttt tgctcaagcc ctcaacaata accgcaagtc
     6901 ttctgggtgt tgcaagaacg tcaatatttt tatattcaac tttgttttct tcaaaaaact
     6961 ttttaaaccc ttcttcaagc tgctctattg cttgcgggat aaacctgtag ggaagttctt
     7021 ccgttcctat ttccagtaaa tattttgaca ttttttcatc cttgaactca attttgttgc
     7081 aagaaaatgg taacttaaaa aaagtatttg ttcaagaata ggttttacat tgttgacata
     7141 ggcagaaaaa cccttcataa taattgcatg ttttcaggcg gaagatttaa aataaaggat
     7201 tttgcaaaga tttctgcatt tacgcttgca gaggttttga tagttatcgg aattattgga
     7261 atgatagctg atttgacatt gcctaatttg ataaaggatt ttgaaaaata acaaactatt
     7321 tcaagactaa aaaaagttta ttctactctt aatcaggctg ttgatcaagc aaaagttgat
     7381 tacggaacca atgttaatga ttgggaattt ggtaaaaatt tggaattggg aggtgttaat
     7441 gttaccctac ccgatggttc attagcctat ggtccattga attatccttc tcaaattttt
     7501 gtaaatactt accttaggcc ttatttaaat gttgcagaag attgcagtta tacaactgcc
     7561 cgtgctgcca atgcttcctg gcgtgaaaaa tgcggggttt acaacacaac ttataaaaac
     7621 tatcctatga aatatctttg tgccgccaga aacaatgctt gtgtttttag caatacaata
     7681 gagactaacc cacagggcgg agcttactca aggcttttat taaaagacgg cactttgctt
     7741 caaatgtata ttgtcaatga gttgagatat gaatatagtg atacaaccac gcccggcgtt
     7801 tgtccgtctc ctgcaccggc gtgtactaaa atggccgctg ttcctgatag ttacaatgat
     7861 cttggagaga tagtgccggg gactccggaa agatgcagat gtcatacaaa tgaatataga
     7921 attcgtgcca atatttatgt tgacttaaat ggaattaagc cgcccaatgt ctttggtaaa
     7981 gatatttttt tattgaattt atctcctagt aatgggacaa ttgttgctta tccaggggtg
     8041 ggtaagactc ggactacatt aaccacaaat acgcctgcag ataatgcgcc ttgcaaatcc
     8101 aaccgttatg gtttgtattg cggggcactt attatttatg acggttggaa aatcgcacct
     8161 gattatcctt ggccgtaaca ggattggact gctatctttg tagcaggtct tgacaatatc
     8221 tgttgttatt gtataataat aaaacggtaa aggaaaattc atgacggcaa aaaactcaac
     8281 acgacgtata agacgtatag gaaaggactt gttttagccc ggagtttttg cttggaaata
     8341 tatttaaaac aagacctttt gaattaaaag aggtcttttt tgttacaaat tatgaaattt
     8401 tcacaaaaaa caggcatgaa taaatattga gaacacttac atttaggtga aggctaaaac
     8461 ccctcacccc aaccctctcc cgcaaggggc tagggggaag taaaaaggca caactatgat
     8521 taaaaatatt cgacgaaatg caatcataaa agctaacgct ccaattatga aattaatgaa
     8581 tttaatttgg ggcttgtgat ttagattgta agccggcaca ggcttttgtg ccaaagaagg
     8641 aaaaatgaca ctcggaacaa ccatatattc aatattagga aacaacgctt ctattattcc
     8701 attagggatt aaagacgtaa gcaacgcact tgggcaaacc gcaggttctt accttgcagg
     8761 agataagata gaaggcaaag acagatttat agatgaattc ggaactcaag caatttggat
     8821 tggcggaata ccgctttata aaaaattaat agattcaacg ctttataaat tattcggatt
     8881 taattcaaaa ataaacgtaa gagtgcttaa agacccagaa attcttaaaa aagcagcaga
     8941 gcaagcaccc gagcatctta aggcagggtt tgaaaaactg cttgaaagcg gtaaaaatca
     9001 aaagattttt aaaggacttt tcagtgcaag atttatagct tcaaccgctc taactcttgc
     9061 ttcttataac gcgcttactt atttcagaca caaaagcacc gaaaaatcag tcttggctga
     9121 aataaaaaga gaagaagaac taaaaaaaac aagtcaaaag tttattgaaa acaaaaataa
     9181 aaaatctttt gataaattcc ctgttgcgtt taaaggcgga tttcaagatt tcatgtttaa
     9241 cccggttaaa aacatgatga taattgatgc cggaatttct tctcaaagac ttataagttc
     9301 aagaaacctt caggatttta tcggatattc tataaaagaa ggtgctttct ggttctttat
     9361 gtattttgcc ggtgcaaaaa tccaaaaaat ctttgaaaac agagctgccg caaaacacaa
     9421 accgattgat ttagacataa gagcattgca aaataaaaat cttaaaactt tatttgaaaa
     9481 cggcacaata gagtcaaaac ttaaagagtt ttctcttgaa ggctctgatg ctaaaattta
     9541 tgaatcttta tttaataata acaatattgt cgttgaaacc gccaaaaaat cagacataat
     9601 taaaacaatg aaaaaaacag gcgaaatcaa cacaggtcac tatatcgaca taaaagaaat
     9661 aaaaggggtt catacaaaac ttcaaaacct tttaaacggc tttaaagaat ttgaagcaaa
     9721 aggaaaaggc ggagttgata aatatttcaa agaagtgacc gatctcaaaa gattatctgt
     9781 actcaaaaac atcggaacct gtataggatt cttggggatt atagtcccag gaaccatgct
     9841 tgttgtaaga cttttgagaa aagataaaga atttcaggtt aaaaaagaga ttaaagaaca
     9901 ggttttgaat aaaaagggct gaaaagttag gagttaggaa ttaggagtga ggagttattt
     9961 tagttgataa gtcgaatagt cgataagttg aaaagttaaa tttattataa ttgcacttat
    10021 ttacttattt acctatttac ttattcacgt ctatttacta ttcaaatact cgcaaatcgc
    10081 acttaatccg agtttataga catttttctt aaatcctgta atttggccta tacaaacagg
    10141 cgcaataaga gaattatgtc tgaattcttc tctggcgtgg acatttgtca tatgcacttc
    10201 tatacaaggt aaatttacag cagaaatggc gtctcttatt gcaacgcttg tatgcgaata
    10261 accgcccggg ttaatcacaa taccgtcaaa tccgaagttt gctttttgaa ttttgtctat
    10321 aatctcgcct tcaatgttac tttgaaaagt ttctatatca acgctaagtt caaaagagta
    10381 agcataaagt tctttctcaa tatcggctag agtttcaaac ccgtattttt cgggctcgcg
    10441 tgtaccgagc atattcaaat taggtccgtt gattattaat attttcatct atacctcgtt
    10501 ttttagttta caatatttac tccaaaatag caattttttg tgtataatta atttatgaaa
    10561 agcgggaaga aaagattaca tcttgacggc aaaaaaaggg ttactctagg gaaattaatc
    10621 tccaaagaca cgaccttttt tgaagttgaa aaaaaatccg acggaacttt aattctaaag
    10681 ccccaatcag cactgaacaa tgatgaggct tggatttata aagataaaaa aacatttaaa
    10741 aatttaaaaa agagtttatt gtcgctcaaa aaagctttaa tcacaaaaat agacaacgac
    10801 ttttgggacg gaatgaacga actgaaagat ttgaaataca caaacgaatt tttaaaaaat
    10861 ttagagccga taatttcaaa aaacaattca aaactctttg aaggcataaa aggctcgctg
    10921 aaaaaaatta tctcaaacaa tgcccaaaaa cttactttaa aaaacctgaa aacacatgaa
    10981 aacaaagagg ttttattgac ttcacttgag ggcaaagagg cagactacaa aatattttgg
    11041 acaacaaaaa attcacaaat tattgttatc aacttaataa aaaagacata aaaaatattt
    11101 tatgataaaa tctgaataaa aataatgaaa aatacgaggt agatatgaat caaagacgtt
    11161 ggtatgaaaa ggatcctatc ctgaaagaag cgttggagct tttaaagctt tcctgcgatg
    11221 agacaaaaac atctgcggca aattttatcc tgcagcttca agaacaggtg gcttcagagg
    11281 ttatcgagca cgtctatgac ataatgacca gatatgaagg gggcggaaaa cgttggtatg
    11341 ataccgatcc tgtgataatg aaggcaatag aacttcttcg ggttgcgcct aaaaaaaccc
    11401 aaagaaaagc tgccttaaaa cttttgttgg cacttgaaga ccaaagtttt gataatctcg
    11461 atttaaaatt ggaagaataa ttacggcaca aaatgaatac aaaccttaaa gatgtgcaga
    11521 atttaaaaga cacaagagga gttgacatcc aaaaagtagg gataaaacac cttgaattgc
    11581 ctttgattat tcaaagaaaa aacgaatcta atcaggttgt aagcgcaaaa gcaggggtaa
    11641 acgtgtcttt gcccagacac tataaaggca ctcacatgtc tcgctttatt gaggttttga
    11701 gcgaatggaa aaataaaaat cttttgggtg ttgatattaa aggctgtctt cacacaatcg
    11761 caaaaaaatt gaatgctcaa agcgcggaac ttgaatttaa tttcaagtat tttatcgata
    11821 aaaaatcccc cgtgagcaaa attttatccc cgatgagcta cgattgttcg tttgaaggaa
    11881 aacttataaa tttcggcaaa aaagatgaag attataaatt tactttaggc gtaaaagtac
    11941 ctgtgacaac gctttgccct tgttcaaaag aaatttcttc tcaaggagcc cataaccaaa
    12001 gggcaattat ttcggttaaa atttcttacg atgaaaacag tcacatctgg attgaagatt
    12061 tgattgaaat gattgaaaaa tgcggttctt gtccgattta tcctcttttg aaaagggcgg
    12121 atgaaaaata tgtaaccgaa aaagcttatc acaacccaaa atttgttgaa gatgtgctta
    12181 gagaagccgt cttgaaattg agagaaaaca aaattataaa ttcgtttgaa gttgaatgcg
    12241 aggcatttga aagcattcac aatcattctg catgggcgta tcaaaaggaa aattagtttt
    12301 ttatttatcg gttttatcta aaaacaaatt tagtgcgtct ttaaaatctt ttggagtttc
    12361 aagctctgag ccgtttacgg tttttgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnagtg cgtctttaaa atcttttgga gtttcaagct ctgagccgtt tacggttttt
    12541 gcgggcatgg tagttttgtt taaaacaaac ttttgataga ttttaagtcc taaaaatatt
    12601 gcaagtgccg atactaaaac gcctgtcatt gcaagcagaa atttaagaat gccgtattta
    12661 aacccgtctt tttccatgtg cgtaagttca taagcatttt tctccgggat tgtagcaggc
    12721 attgcacttg attgcgccgg aatagtttgt gtaacaggcg cattttgcga catgggtgca
    12781 ttaggctctg ttgcgcaggc aaaaagtgcc gttgaaaaca ttaaaaataa aaatgaaaat
    12841 aataaattaa gccataactt gttcttcatg tttcacctct tgtttttgaa tttttttagg
    12901 aacacgtttt tttatgcttt ttacaggttt ttcttcaacc gttttttttg ttttttgttt
    12961 tgacggtttg ggcgagatta cttcttgtat aacttcttta attacttctt gaaggtgtgt
    13021 ttcatgccca gaagtatttt cttgtattga agttgtttca ggctgtcggt aatctttttt
    13081 cttgttttta ttaaatctgt ggtttttgtt tttgttaaac cttttttctt ctttttgtgg
    13141 cgtttcttct tttgcttgct gttcaacaag gctttcttta ggcaaaattt cttcttgagg
    13201 ttgattttga tcattgtttt gattgcggtc aaattgtttt ttgaaattac cttgttgttt
    13261 tctacgctta ggctgcggct gattttgtgt aaactcactt tctataatcg gagcacaaaa
    13321 gtgcagttct tccattatat agccgtttcc ttgacagtgc gggcattttt ttgcaaaaag
    13381 ttctgcaagg ctttgtcctt gtctgtgtct tgtaagttca acaagtccta agtccgaaag
    13441 ctgtccgatt tgaggctttg ctttatcggg tgcaagcgcg agttcaagtt cttctaaaat
    13501 cgcaagttta tcaacgcgtg aagtcatatc gataaagtcg ataattatca tcccgcctat
    13561 atttcttaat tttaattgtc ttgcaatttc ttttatcgcc tctttatttg tttttaaaat
    13621 ggtttcatcc tgagaagaag aacttatgaa cttaccgctg tttacgtcaa taaccgtaag
    13681 tgcttctgtt gtctggacat aaagatagcc gcccgaggga aggtttactt tgggttgaag
    13741 cgaagcttta atttctttgt gaatatccat agtagttaaa agagggtctt tgcctctata
    13801 gagtgtgact tgaatatttt tgttcatatg ccagttttga agaagatgtt taaccctgtt
    13861 cattgcaaat gatgtgtcaa cgacaatttc tgaaatatct tctgtgcagg cttctcttat
    13921 gactctgtac aaaaggtctt ggtctctgta gagcaaatta ggaggggtta gagtgtcggc
    13981 ggatgttact atgttgttcc acttttccaa taaaacttct aaatcttcct gaatatctgc
    14041 ttctgattgt ccttcggctt ctgttcttat aataattcct acaccaacag gttttaaaag
    14101 gcttacaagt gctttaagcc ttgctctttc tttggaagat tctatttttt tgcttacgtt
    14161 aactcctgtg tcattaggta ttaaaaccaa aaatcttccc ggaaggctta cttccgttgt
    14221 gaccctgggg cctttgtgcc ctgtgggctc ttttacaacc tgaacaacaa gtttttgctt
    14281 gggagaaagt ttttctctca gggaaccttt gcccatgacg tcttctgcgt gtaaaaaccc
    14341 cattttgtcc ccgccgacat caacaaatgc agcgtcaata ctgggtaaaa tgttgtcaac
    14401 ttttgcaagg taaatgtctc cgagcaaaac gtcacccctg tgtacgaaaa attccataat
    14461 ctttttgcct tccataacaa cggcaatatt atcgcgctcg gcgataataa ttttctttct
    14521 tttgttttcc ataaaaccct ttttctttct tgcaaatagt taatatttgc ttttgtgtct
    14581 aaactttttt atatttcatt catatctcgg tcaaaaaact tgacgcgctt gatatcaaac
    14641 aaaatatcgg gtgcgatgat tttcatcaaa tcatctgccc tgagcgcagg aacatctgtt
    14701 ccctgccctg tttttaaaac tatgaacaaa caatcatcaa taaattcata agatttaatt
    14761 gccgatttta tgtttgttgt tttagttaaa ccttttttgt ttttcttcgt cagcaaaatt
    14821 tcttctgaag ataaaactct atccatattg tagcgcaaac tttcaaaatt ttgaagtgta
    14881 ttgtcaaaag gcttgatttc atattgtgcc cactgaacag tattgtcgat tgcttgggct
    14941 gatttatcaa tcttaacaat tgattttatt ttgcaacctt cgggcaaaac tttctccaac
    15001 tttagtttga cttcttcttt acaaacatcg tctaaaagtt ctatatcgac aagttcgcat
    15061 ccgctttcaa tgaacaaagg aagcgcgacg cctaaagaaa ccttcattgt agggttaaac
    15121 ccttgcgaat aagcaacttt aagccctgag cgagaaagtg ctttgtgaaa tgtgttttgc
    15181 cagtcaaggt gggaaaagta gcgtaagatg cctgattttg taagcttaat ccgatattta
    15241 gttgaaaagt cgaatagttg aaaagtcgaa cagttaattt tgttatcctg cgcttgttgc
    15301 gggattttag cattgtattt tttatccata acttttttaa ctttaaaatt attacacact
    15361 ccgcagttta cgcagtttgt ttggcatgaa ggatttgttt tgcttttgag tgcgtcttta
    15421 tattcgttta tcagccaatc tttttctacc ccgatatcaa taaaatccca aggcaaatct
    15481 tggtttagtt catattcttt ttgagataat tcgtccaaag aaatttccac tttttttgca
    15541 cattcaagcc aaatattctt gtcaaaatat tcgccccaag catcaagata gcaccctgct
    15601 ttaaaaagtt cttcgatgta ggcgcaaagt tttttgtcgc ctcttgttaa aaccgcctca
    15661 atttgagagg tgaatttgtc atgataattt atttttaaac cttttatgtg tttggttttg
    15721 tctttaagat aataaattct tgaagagatt tcatccagac ttggctgttg cgcccattga
    15781 aaaggcgtga atggtttagg tacaaaaatc gaaagagtgc aggttatatc aagggagtgt
    15841 tttagttctt tttctttttt caaaagtttt gccctgtctt ttattttttc aagcaaagcc
    15901 gccatctcat ccaagtcttg ataggtttct gtgggaagtc ctacgatgaa ataaaacttg
    15961 atttttgacc agccgttttc atacaaagta agtgcagcat tcaaaatttg ttcttctgta
    16021 atattttttc tgataacatc tctgagcctt tggcttcctg cttcgggtgc aagtgtcatg
    16081 gtggattttc gaacgctttg aacgaggttt gcaagttcaa ggttaaaccc gtcaatcctt
    16141 tggcttggaa gcgaaacgga tatttttttc tcgttaaaat cgacacaaag ttcttttatc
    16201 acctctttta tgtttgaata gtcatttgag gataaagaaa gaagcgaata ttcatcatag
    16261 cctgtgtttt tgaccaattc ttttgcaatt tttattatat cttgggcttt tctttctctg
    16321 acaggcagat taacgtgccc cggctggcaa aacctgcaca tgcgcccgca gccacgtctt
    16381 atctcaacaa tcgccctgtc atggacacaa gatgaaaacg gtatgggata agtcttaagc
    16441 gcattttcat aatttatctg tacaattctt tttttaattt tctggggtaa atgatttgaa
    16501 gatttattca tccccctctc cccctgaggg agagggttgg ggtgaggggt tttcccaaaa
    16561 taaagctgag gcacaaaaac gccttccagt tgtgccagtt ttgctatttt ctcggcgcgg
    16621 caaagccctt gggctttgcc gcgctgaagc acttcacaca cttccaaaat caaatcttca
    16681 ccgtcgccta ttaaaaacgc atcaacaaaa tcagccagag gcaaggggtt gtatgcgcaa
    16741 ggtccgccgg caacaattat cggctcatct tggctccttt ggtttgattt tatgtttatt
    16801 tttcccattt taagcattgc aagcacggtc ggataagaaa gttcatattg aagtgaaaat
    16861 cctataatgt caaagttttt cataggtatt tttgcttcaa gagaataaag ctcttttttt
    16921 gttttgatta aagctttttt aaaatcagct tcgggggcat aaactctgtc tgccataaag
    16981 tccggattat cgtttacaag cccgtataag attcgctgcc ccagattgct tattccgatt
    17041 tcatatttgt cggggaaggc aaacagaaag cgtatttttg cctcgtcaaa atttttgttg
    17101 aaagacaaaa attcgccgcc aacgtactgg taaggcttgt tgcagttgta caaaagtttg
    17161 tgatattctt caaaaaagtt tttcatatga ttttcaaaac cttgcgtcat tgcgagcatt
    17221 agcgaagcaa tccaaataat gctttttctt ggattgccac gtcgccgtgc tcctcgcaat
    17281 gacaagaatt atattaaata ctcactttac gctaaatctt ccggaaagta tttatcaatt
    17341 tcaattattg taccatttaa agtattgttt tcaaacgctt ttataaattc aaaaagttga
    17401 tcattggaaa tgtcgtaatt atataacaaa acttcgttat caatatttct cataaccctt
    17461 actatgtaat ggcatttttg ggcatattta ttcaagtgtt cggcattaaa ttttttaccg
    17521 ttattgtctc tgtcaagtct tacgtaatca aatcctgcaa aaaactttac cctttcttct
    17581 tcttttaagg ggatatcttt tttgtgtttt gaaaaaatac ttataacttt tttgttaatt
    17641 tcatctgtca tattataaat ttagcaacat ttgccttaaa agtccaaatg taacggaaaa
    17701 agtgaaaagt gataggtgag aagtgagaag atatttgata attaaagtaa tttattcaat
    17761 aaaaaccagt taaaaatctt tacaattagc ctgataacaa aaaaagatat ataattttaa
    17821 atatacgatt tgatatacaa ggcggaaata tgttgccaat tattacagaa gaacattcat
    17881 cgttagcgtt tgcagaaatt tttcaagacg ttcacagttg gcgcaaaaaa atgattcatt
    17941 atttgaaaga agaaaacccc gaaattaaca ctgccataat tgaagccgca aacaacactg
    18001 atttagaccc caaagccgta gcactcggtg cttatatgac ctatgttttg cttgaaatgg
    18061 caataaaaga tgatgaaata attttaggcg gcgaagaaga ataaatttta tttttgactt
    18121 ttatatccga agttttttag catttgctct ttttttcgcc agtctttttc gacttttacg
    18181 ttcagttcaa gataaacttt tttctcaacg atttgctcta attctaaacg tgcctgagtc
    18241 ccgattattt ttaaaagcga gccgcctttt cctattaaaa tccctttttg gcttggggtc
    18301 tcgcagtaaa tatttgcaaa aatcctgtca atttggggtt tttcttcgta attttcaatt
    18361 ttcaccgcaa ccgagtgagg gatttcttct tttgtgttaa tcaaaatctt ttccctgata
    18421 atttcgcttg caacatctct catggtttct tcggttacaa catcttcatc gtaaagcttt
    18481 tgaccttcgg ggagtagatt taaaatttca taaattaatt tttctttgcc cttgccggtt
    18541 tttgcagaca gctgaatggt tgaatggctt aacggctgaa tagttaaatt tttgtaggtt
    18601 tctaaatttg catttatttt ttcagggttt gttttgtcga ttttgtttaa gactaaaaga
    18661 acaggggttt ttgattggtt taaaatattt tcgacaatcc atttgtcgcc tttgcccgaa
    18721 ggttctgagc cgtcaaccaa aaacaggatt aaatcggcat ccggaatggc aatttttgct
    18781 tcatcaagca aaaattcacc caatttatta aggggtttgt gcacgccggg ggtgtctata
    18841 aatacaatct gaccttcgtt ggtggtgaga atccctttaa ttctttttct tgtggtttgg
    18901 gctttgtcag tagttatgac aattttttgc cctaaaattt ggtttagcat ggttgattta
    18961 ccgacattcg gtctgcctat tattgcaaca aagcctgatt tgtaagtgtt tttattcata
    19021 aaatttatta tataacaaat atttttcacg tcgttgagag ggggtaaatg tattttgact
    19081 tttgttcgct ctcttacgtc gttgcgagga agaaatttat ttctgacgaa gcaatctaaa
    19141 aaac
//
LOCUS       DAOQAU010000033        18068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_3252_length_18068_cov_6.939766, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000033 DAOQAU010000000
VERSION     DAOQAU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_18068_cov_6.939766"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PKI94_05850"
     CDS             <1..995
                     /locus_tag="PKI94_05850"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNW26298.1"
                     /translation="EQREIDNLSEFATKSRFSVGRKKQEDSCPLRTDFQRDRDRIVHS
                     KAFRRLKHKTQVFLSPFDDHFRTRLTHTLEVSQIGRTIARALNLNEDLVEAISLGHDL
                     GHTPFGHCGEGVLDELMPGGFHHNLQSVRVVEVLEDLNLCQETIDGILTHTWGFQPKT
                     PEAQIVQLADKIAYINHDIEDSIRAGIISESDLPQDCIKYFSSIQKKRLNKMIEEIVE
                     NSIGKPTVTMSEEGWAYTTKLRQWMFEHVYIDSPAKLEEGKAKKIIKELFNYYVELLT
                     PMCNEEKTQRIVCDYISGMTDRYAIEKFKELFIPEPMIHKTNDDYLFRLAKLIN"
     gene            complement(1067..2728)
                     /locus_tag="PKI94_05855"
     CDS             complement(1067..2728)
                     /locus_tag="PKI94_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNW26299.1"
                     /translation="MDQTNVLLFLENRTEVLDNKIALGMKSNIGWSELTFKGLGLLSR
                     RLANHLIRIGVDKGDRVAILSESMPEWGAALFASVLSGAITIPLDIKLTIYELTSILN
                     DCKPKVLLVSNAYLELAIKLKEEISSIEQIVILNESPASSGHLSLHNIEDVPDRKWRH
                     RAVSKTAFVIYTSGTTGTPKGVEISFKNVMAQMDSVTQCFDLGINDKFLSILPMNHLF
                     ELTVGFLSFLSKGTSIYYSQSLKPKDLFNIIQEKQITFMIVVPAFLKLLKSSIENEIN
                     QAPKLKKLIFKGMYNLASIVPLTGFRKFLFSGIHKKFGGKFKGCISGGAPLDVEVGKF
                     FNILGIPVFEGYGLSEASPVVATNRHGKNKLGSIGPAIPNVQVRIDPQTGELLVKGDN
                     VMKGYYNLPELTAQVIDQDGWLHTGDIARIDKDGYIFITGRIKSMIVLSGGKKVFPEE
                     VEAVLEKSPKFAEICVFGAKRFGGQKDGTEDVFVAVMPSEKILSSGLSDEDINNEIRA
                     EVKKLSEKLSSYKRPTNVIVVKDPFPRTATKKVKRNEVKKELLGV"
     gene            complement(2812..4626)
                     /locus_tag="PKI94_05860"
     CDS             complement(2812..4626)
                     /locus_tag="PKI94_05860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNW26300.1"
                     /translation="MKKISVSKKVVNNLVYFFSFMAIILMIFLFFKIAKINDFFTLIE
                     NRTFDWRQTLLSNSGHKKVNDNIVIITVDDASFEYLLEKYGEWPIPRDVYANVIYYLE
                     KQKPKTIAFDFMFVKSMKSRPAADLAVSKAINDNPNVFVGMNFDNQSYDIRKPTNLPS
                     RFSIKLKNDASIDFYKELEFSNCRSILSQLLNGKANIGIINASRADDGILRKMPPFLI
                     YQREFYPHLSLLVALDYLKKTENLDLKDFHIDKHKNMQIGKRTIPFDSDGGVILNWYA
                     PAGKAFKYVPLYKVIKAMEKNQKMTDIDFKDKLIYLGTTAVSLYDTKSVPVDKLYPGV
                     EVHTTFINNLLDNNFIKKADEFVNIAISLVLALIVSIIVISTASTFISLSSAILITAL
                     YLIFSYYIMKFANIWVGIVMPITAIVLMFAFAYIVKYLLKSRDFEHQYRLATTDGLTD
                     LYNHRFFQEQMIMQVENCRRYNSHFSLILIDIDYFKKFNDKFGHQSGDAVLRQVAQVL
                     KKNVRSTDVVCRYGGEEMSIILPNTDRDEVIITAQKICQAVAAKPFKLGSDIESIVTI
                     SLGVSTYPQDGNKPSELIDKADQGLYNAKENGRNQVGI"
     gene            complement(4651..4818)
                     /locus_tag="PKI94_05865"
     CDS             complement(4651..4818)
                     /locus_tag="PKI94_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26301.1"
                     /translation="MNLHEECLLRYLKAPDELSFSTDVLKINNQILEQHFLLENFLTK
                     NIKLNYNNHVK"
     gene            5066..5266
                     /gene="rpmE"
                     /locus_tag="PKI94_05870"
     CDS             5066..5266
                     /gene="rpmE"
                     /locus_tag="PKI94_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNW26302.1"
                     /translation="MKKGIHPDYKKTTVKCVCGNEIETGSIEEGITVEICNNCHPLYT
                     GQQKILDSEGRVERFNKRYGKK"
     gene            5355..6053
                     /gene="thyX"
                     /locus_tag="PKI94_05875"
     CDS             5355..6053
                     /gene="thyX"
                     /locus_tag="PKI94_05875"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNW26303.1"
                     /translation="MSKNKPVVNLISKPENMLKTVYTACRTCYSADYPINIYNSTDDE
                     EKMLKLIERVISSGHYSTIEHIQISFAIANVSRACTHQLVRHRHMSFSQKSQRYVKEK
                     GQFDYIIPPTIEKNPELKEKFENFMGEISGLYNEFTEAGIPAEDARFVLPNAAASSMV
                     ASLNLREMIHLANLRLCTRAQYEIRILVKMMCEELIKQEPWLKPYLVPKCERLGFCDE
                     DKSCGRVQIKKQLI"
     gene            complement(6056..7633)
                     /locus_tag="PKI94_05880"
     CDS             complement(6056..7633)
                     /locus_tag="PKI94_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26304.1"
                     /translation="MSMNNIAIRANTFKSINIKPKQDSLKPSVLEQKSNSCCSAMQLN
                     NNKDLVNFTGLNRVLSKNTYEYTINIEELSEIYPKSNGIVGNLPPAWIAKIPKENREK
                     VIKELFNDLKPIINEFRESENEQFMQERIKTALIQAGIVKNQENFKMEFLGEGTYGKA
                     YKLNNILGADYIIKIFKKLNKNLDKGLDDMHGNYIEQSRALYWQKQAGFNTQMIRFYF
                     GDIDAGYMVNKFIDEDTPKSDKFVYPEIVGLHSSDIGKEYNEINGTQYDWGGFRIIIS
                     SQRIKDKKIKKLIKDILEKPVNQRKEIFEQYFTSTDKGIKLVLSSMIEYLPEHQRARY
                     FHNLLENADSEVKNALAARIYNLPEEERTSCFRELLENPSNGSVENLAYGIYCLPLKE
                     TAACFKLLAKNPSNRAKAALINRIYCLPEEERGFYFDQFLKESDIKVKKTIVENITCL
                     PQKERLFYFYQLLKNADANLKKVLAFNIDCLQDEERTACFRKLAKNANSEIKQILADR
                     ICYLPKEDRQEVQNLVS"
     gene            7794..8873
                     /gene="prfA"
                     /locus_tag="PKI94_05885"
     CDS             7794..8873
                     /gene="prfA"
                     /locus_tag="PKI94_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNW26305.1"
                     /translation="MKDMLDKILQIEQKYMELGETLSKPEVIQDYNKFKELSKQRKAM
                     EETVELYHAWKKATDAVKEAQELIWAEKDEEMKEFLKAEMEENEAKIPQYEEQMKVLL
                     LPKDPMDDKDIMLEIRGGAGGDEANIFAGDLFRMYIKYFDKQGWQAQLLSKTDCEAGG
                     FSEVIVSVKGENVYSKLKYESGVHRVQRVPATESQGRVHTSTATVAVMPEIDDVEVEL
                     RQEDLEISTARSGGAGGQNVNKVETAVRVVHKPTGLMVFCTEERSQLQNKERALQIIK
                     TRLYDMEVQKQMQEVTDLRRSQVGTGDRSERIRTYNFPQGRLTDHRINQNLNVWTVIE
                     GELDELIKLLIEYDQKLKLEKLAKG"
     gene            8912..9418
                     /locus_tag="PKI94_05890"
     CDS             8912..9418
                     /locus_tag="PKI94_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26306.1"
                     /translation="MEDDKCEGCRYLLEKHFQYYQTQYIESSKYTSTIITVAYASLIS
                     FLILTYNYINDFALLVILGFLIISILLFVIQLIWNMELMRKSTEIMNELWQEYWQEKI
                     KLNDLIQGIYTKNSEAKKEFNRWNNFLFQWTLITGILSALVLLICIFTTLISPVMLNS
                     FQHLSSVR"
     gene            complement(9772..10080)
                     /locus_tag="PKI94_05895"
     CDS             complement(9772..10080)
                     /locus_tag="PKI94_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HNW26307.1"
                     /translation="MTKKIKNTGKLENLISDSLKTEDDIRDWLTVSLEEYLEDNDFSA
                     FYHSLEYAIKAKDSISGISKKTGISRSNLYAIFKGEVQPQMSTVMKILKELGYKLKVA
                     "
     gene            complement(10073..10396)
                     /locus_tag="PKI94_05900"
     CDS             complement(10073..10396)
                     /locus_tag="PKI94_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNW26308.1"
                     /translation="MDKRKVLYLKLNRKEPLKDWLKSLDKIVAKRILQRLIRIEEGNL
                     GDFKKIDDEISELRFMFGPGYRIYYTEINNVILLLINGGNKSTQSKDIVKAKELLEQW
                     RQEND"
     gene            10461..10718
                     /locus_tag="PKI94_05905"
     CDS             10461..10718
                     /locus_tag="PKI94_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNW26309.1"
                     /translation="MKKEVLRKCIGCFESKNRDGMIKITRQHSSNEIFINPNSKIFGR
                     SVYLCYNNSCIENSLKKNKLAKFLKANFNEEFKERLRTMIS"
     gene            10741..13218
                     /gene="infB"
                     /locus_tag="PKI94_05910"
     CDS             10741..13218
                     /gene="infB"
                     /locus_tag="PKI94_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNW26310.1"
                     /translation="MTLDTMRVNELAKELNLTNKEIIDKFAQLSIVIKSHSSTVTGDQ
                     IKKLKDFIEAGSKVEVKKPKAFIVKKVRTEAPAEPPKEEEKPIVKAEIEKPKIEVVKT
                     VPNRLEIVRKAPAQREFPSRPSAPIHKKPQEKIEKSTQTKDEAETSTEISTEKKTDGA
                     VVEKTERRQPIQRHIISQDIYNNKGGIKRKVEKTKQKVFTTKEEEQERISLEKAAAQK
                     HKKRSQREEEVEQVTKLVVNQPMTISELAVKIQKTPAEIVKFLMLQGVMATVNQLIDV
                     ETIKKVCNEFELEVLDEDLEAFVEQELEKEEKAKALQAVDKKLLKPRAPVVSIMGHVD
                     HGKTTLLDSIRASKHKIVATEVGGITQSIGAYTVFVDKKKVVFIDTPGHEAFTEMRAR
                     GAKSTDIAILVVAADDGIMPQTIEAISHAKAAETPIIVAVNKIDKEGANPDRVLQQLT
                     EHGLVPEEWGGETIVVKVSALNGTGIDELLEYILLLAEMADLKANPKAHASGVVIEAN
                     LDKGKGPVATLLVQNGTLRVGNCLVVGSVGGRVRALLSDAGERVAKAEPSTPVEILGL
                     TEVPQAGDYFEVLDNEKAMKSIVDKRKENERSRRLDAMLPAHIRKESAAGDEGITQLN
                     LIIKANTHGSAEAVAQAVAQLESKEIATKIIHVGVGDISEADVMLASASGAIILGFTV
                     KEDSNALAAAEKEGVVIKKYDIIYQVLEDLEKTMLSLLQPEIKHVEIGKAEVRQIFTI
                     GKTAKIAGCYVTEGKIQRGKTAVLMRDGEEIFKGVIDQLKRFKDDVKEVKEGFECGIS
                     FAKFNDIQEGDIIEVSTTEEIERVSLD"
     gene            complement(13335..13697)
                     /locus_tag="PKI94_05915"
     CDS             complement(13335..13697)
                     /locus_tag="PKI94_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26311.1"
                     /translation="MLTNSINADYQSKQNFTGLKEGKSFRRIANKLILQLKEIPEGTF
                     GYNVRTNGGTSIKDRLYSAIFDKAQKIGDEFVTTEDRRLHNQLKDLYKNYTESDDLIN
                     GLGGGQNFFVDHTINFKI"
     gene            13843..14187
                     /gene="rbfA"
                     /locus_tag="PKI94_05920"
     CDS             13843..14187
                     /gene="rbfA"
                     /locus_tag="PKI94_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNW26312.1"
                     /translation="MTLRNERVRKELMREIADILQKEIRTAGMTSIMDVEVSHDNSFA
                     KVYYSVFGSDEQKQKTIETIEKNTSKIRYEIGKRIRLRLTPELRFIYDDSIERGTKVT
                     DLINKISRGEIK"
     gene            14322..15182
                     /gene="truB"
                     /locus_tag="PKI94_05925"
     CDS             14322..15182
                     /gene="truB"
                     /locus_tag="PKI94_05925"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNW26313.1"
                     /translation="MFGFLNVYKPTGMTSHDVISVLRKISGIKQIGHSGTLDPFAQGV
                     LPIAIGKATRLIEYLNDDKEYLAEISFGKATDTYDCDGRAISECNSEIALNDLQKALK
                     TFEGEIFQTPPIYSAIKVKGKKLYEYARKGQEIEIEPRKVFIERIELKSFDEESQKAQ
                     VLIKCSKGTYIRSIAHDLGQNLKVGAHLSELIRTKAGDFTVDKAIFLDEIKTKEDIEK
                     NLINPLKMLNLTQIILNEIEYKKITMGQFIDNKNSLQNDDLIILIYNNEIKAVANVDG
                     NLIKVKKVLV"
     gene            15179..16036
                     /locus_tag="PKI94_05930"
     CDS             15179..16036
                     /locus_tag="PKI94_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26314.1"
                     /translation="MKKLVLLIAAVLFFGNSAWAGCDYTSKCSPAAYGNVSPSWQRVS
                     KYTGATFLTEQIANSIIKKELKKATKENFKVHIKSYSAKDLAQGRFKSLQISGKNLEI
                     ENAYITLFELKTLCDFNYVEFGKDYLKFKENMVMDFTMEISDFDLRRTMKSSGYLDML
                     NNTNISALGLTLLKLENADVKIKNNKLFFTLKVSSKLLPMPVDMILKADLKVEDGQIV
                     LTKVDFVNTYTRMDLSKAAYLLNILNPLTFSTDILENKKSKLSVQNVNIIGDKIIING
                     LVFIPKNTK"
     gene            16046..16612
                     /locus_tag="PKI94_05935"
     CDS             16046..16612
                     /locus_tag="PKI94_05935"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNW26315.1"
                     /translation="MKTWQKISLILLVFATIVYIKLAFFTGSEVAPPTDETAQEQEEA
                     QKMPKIYTYIFFVGQNADKEEVYKAVKREYPEDTDDSKLKFALTNLLKGPSAKERTKG
                     VYTEIPSGTRLLSIEELPNKIIINLSSDFETGGGTDSLYKRLYQLIKTVNKNSDTPVY
                     LQLNGKQIDVIGGEGLMLNQPLNERSLD"
     gene            16618..16887
                     /locus_tag="PKI94_05940"
     CDS             16618..16887
                     /locus_tag="PKI94_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26316.1"
                     /translation="MTKDLDYYLNLNWTLIEGQDLDFNGQPYHFIEIKEIPSFAFCAK
                     TIQKARENYKTQLKWTLQVMLESGEKIIEPGEESDDQIDWESLCP"
     gene            complement(16952..17851)
                     /locus_tag="PKI94_05945"
     CDS             complement(16952..17851)
                     /locus_tag="PKI94_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26317.1"
                     /translation="MGMAASQARYLSLTARKSNVEYEGQQINQQRTNLANQSATLNRQ
                     LLDLSVPTPPSVSAYAQTQYSITMNGENCNITNLFKKPGGLYNVSYTYQDTNTMIVPS
                     SQYSVTGSSGSYSIGGVALVLVPSGSEAETQLNDAAQIGNGPYYSYTATNGAITYFSQ
                     ADIDAAIAANDQTQGYIPQEFEYEHEAYLEDVTVTFSGDQPVSITLSNGTQISMNATS
                     NVNEQEYDTAMTQYEYNKAQYEKQMEDINSKLSIIQQQDKTLELKLKQADTEHNAIQT
                     EIEAVQKVIGKNIESSFKTFGNG"
BASE COUNT         6245 a         3182 c         3078 g         5563 t
ORIGIN      
        1 tggagcagcg cgaaatcgat aacctgagcg aatttgccac aaaaagcaga ttttctgtcg
       61 gcagaaaaaa acaggaagac tcatgtcctt taaggaccga ttttcagcgc gacagagaca
      121 gaattgtaca ttccaaagct tttagacgcc ttaagcataa aacacaagtg tttttatcgc
      181 cttttgacga tcatttcaga acccgtttga cacacactct tgaagtttct caaataggaa
      241 gaacaatcgc acgcgcgctt aacttaaatg aagacttggt cgaagcgatt tctttaggcc
      301 atgatttggg gcatacgcct ttcggacact gcggagaagg cgttttggat gagcttatgc
      361 cgggtggctt tcaccataat ctgcaaagcg ttcgtgtggt tgaagttctg gaagatttaa
      421 acctctgtca ggaaaccatt gacggaattt taacccatac atggggtttt cagcccaaaa
      481 cccctgaggc gcaaatcgta cagcttgcag ataaaattgc ctacattaat cacgatatag
      541 aagactcaat ccgcgcagga attatctcag aaagcgacct tccacaagat tgcataaaat
      601 atttttcttc aatccagaaa aaaaggctta acaaaatgat tgaagaaatt gtcgaaaatt
      661 caatcggaaa acctactgtg acaatgagcg aagaaggctg ggcttacacg acaaaactaa
      721 gacaatggat gttcgaacac gtttatattg attctcccgc taaacttgaa gagggcaaag
      781 ccaaaaaaat aatcaaagaa ttgttcaatt actacgtgga gcttttaact cccatgtgca
      841 acgaagaaaa aacccaaaga atagtctgtg actacatttc gggcatgaca gaccgctatg
      901 cgattgaaaa atttaaagaa ctatttatcc ctgaaccgat gattcataaa acaaacgacg
      961 attatctttt caggctggca aaattgatta attaaaataa aaaattgggg cggatttttc
     1021 aaagaaaaat ccgcccctct taactctcat ttcttctcta ttatcctcag actcccaaga
     1081 gttctttttt aacttcattt cttttaactt tttttgttgc ggtccttgga aacggatctt
     1141 taaccacaat aacatttgta ggccgcttat aagaagaaag tttttccgac agttttttaa
     1201 cttccgctct tatttcgttg ttaatatctt catcgcttaa gccactacta agaatttttt
     1261 cagaaggcat aacagcaaca aaaacatctt ctgtaccgtc tttttgcccg ccgaaacgtt
     1321 ttgcgccaaa gacacaaatt tctgcaaatt tcgggctttt ttccaaaaca gcttccactt
     1381 cctcggggaa tactttttta ccgcctgata gaacaatcat gctcttaatt ctgccggtaa
     1441 taaagatata gccatcctta tcaattcttg cgatatcgcc tgtgtgaagc caaccgtctt
     1501 ggtcgataac ttgagcggta agttcaggta aattataata acctttcatt acattatcgc
     1561 cttttaccaa aagttctccg gtctgagggt caatcctgac ttgaacatta ggaatagcag
     1621 gtccgatgga accaagttta ttttttccat gccgatttgt cgctacaaca gggcttgcct
     1681 ctgaaagtcc gtaaccttca aaaacaggta ttcctaaaat attaaagaat tttccgactt
     1741 caacatctaa aggcgcaccg cctgaaatac aacccttgaa tttgccgcca aattttttgt
     1801 gaatacccga aaacaaaaac tttctaaatc ctgtcagcgg cacgatgctt gctaaattgt
     1861 acattccttt aaatataagc tttttaagct tcggtgcctg atttatttcg ttttcaatgc
     1921 ttgattttaa aagttttaag aaagccggaa caacaatcat gaaagtaatt tgtttttctt
     1981 ggattatgtt aaacaaatcc ttcggcttta agctttgaga gtagtatata gaagttcctt
     2041 tgctcaaaaa cgacaagaaa cctacagtta attcaaacaa atggttcatt ggcaaaattg
     2101 acaaaaattt gtcgttaatt cccaaatcaa agcattgtgt aaccgaatcc atttgcgcca
     2161 taacattttt aaaagatatt tcaacccctt ttggagttcc ggttgttcct gaagtataaa
     2221 ttacaaaagc cgttttgctt actgctctgt gtcgccattt tctgtcaggt acatcttcaa
     2281 tattatgcaa actcaaatga cctgaacttg caggactttc gttaagaata acgatttgtt
     2341 ctatagaaga aatttcttct ttaagtttta ttgccaattc aagatacgca tttgaaacaa
     2401 gcaaaacttt cggcttgcaa tcatttaaaa ttgaagtcaa ctcataaatg gttaatttta
     2461 tgtcaagcgg gattgtaatc gcacctgata aaaccgatgc gaaaagtgct gcaccccatt
     2521 caggcatgga ttcggataaa atcgcaaccc tatcgccttt atcaacgcct attcttataa
     2581 ggtgattggc aagccttctg gacaaaagcc ctaggccttt aaaagtaagc tcactccaac
     2641 cgatatttga tttcatgccc aaagcaatct tattatccaa tacttcggtt ctgttttcca
     2701 aaaacaaaag aacattagtt tgatccatac atccccctat ttataactcc tacacaacat
     2761 tgaaacaata ctatcactaa aatgtttctt tgcaaatatt tggtcaagcg tttaaattcc
     2821 tacttgattt cttccgtttt ctttggcgtt ataaagtccc tgatcggctt tatctataag
     2881 ctcgctgggc ttatttccgt cttgaggata agttgaaact ccaaggctta ttgtaacaat
     2941 actttcaatg tctgaaccca gtttaaatgg ttttgccgct actgcctggc agattttttg
     3001 tgcagtaatt ataacctcat ctctatctgt attgggaaga ataatactca tttcctcgcc
     3061 accgtacctg caaacaacat ctgtagaacg gacatttttt ttaagcactt gcgcaacttg
     3121 ccttaaaaca gcgtctcctg attggtgacc gaatttatca ttaaactttt taaaataatc
     3181 aatatctatt aaaataagcg aaaaatgtga attataacgc ctgcaatttt ctacttgcat
     3241 aatcatttgc tcttgaaaga atctgtgatt atacaaatca gttaacccat ctgtggttgc
     3301 aagtctgtac tgatgttcaa aatctcttga tttaagcaaa tattttacta tataagcaaa
     3361 agcgaacata agaactatag ctgttatggg cataacaatc ccaacccaga tatttgcaaa
     3421 tttcataatg taataagaaa aaattaagta caaagccgtt atcaaaatag ccgaacttag
     3481 agaaataaac gtggatgccg tgcttataac aattatgctg acaattaatg ccaagaccaa
     3541 acttattgct atatttacaa attcatccgc ttttttaatg aaattattat caagtaaatt
     3601 gtttataaac gttgtatgta cttccacccc cggatataat ttatcaacgg gaacactttt
     3661 tgtatcgtac aaacttactg cggtagtacc taaataaata agcttatctt taaaatctat
     3721 atcggtcatt ttttgatttt tttccatagc tttaattact ttgtaaagcg gaacatattt
     3781 gaaagccttt cctgcagggg cataccagtt aaggattact ccgccgtcag aatcaaacgg
     3841 aattgttctt ttacctattt gcatattttt gtgtttatca atgtgaaaat cttttaaatc
     3901 aaggttttct gtttttttaa ggtaatcaag tgcgaccaaa agagaaagat gaggataaaa
     3961 ctccctttga taaatcaaaa acggcggcat ttttctcaaa attccatcat cggctcttga
     4021 tgcatttatt atccctatat ttgcctttcc atttaaaagt tgagataaaa ttgacctgca
     4081 attactgaac tccaattctt tataaaaatc tattgaggcg tcatttttta attttattga
     4141 aaatcttgag ggtaaattgg tcggctttct tatatcgtaa gactgattat caaaattcat
     4201 tcctacgaaa acgttgggat tatcatttat cgctttagag accgccaaat cagcagcggg
     4261 tctgcttttc attgatttta caaacataaa atcaaaagcg attgttttag gtttttgttt
     4321 ttcaagataa tatattacat tggcataaac atctcttgga ataggccact cgccgtactt
     4381 ttcgagaaga tattcaaaac tggcatcatc taccgttatt atgacaatat tatcattaac
     4441 ttttttgtgt cctgaatttg aaagcaaagt ttgacgccag tcaaaggttc tgttttctat
     4501 aagcgtgaaa aaatcgttta tttttgcgat tttaaagaaa agaaaaatca ttaatattat
     4561 cgccataaaa gaaaaaaagt aaactaaatt gttaactact tttttactta cagaaatctt
     4621 tttcaaaaca acacctcaac gctaaaactt ttatttaaca tggttattat aattcaattt
     4681 aatatttttt gtaagaaaat tttccaacaa aaaatgctgt tctaaaattt ggttgttaat
     4741 tttcagaaca tccgtcgaaa acgaaagttc atcaggggct ttgagatatc tcagaagaca
     4801 ttcctcgtgc agattcatat aattcctttt taaaatgact tcaaatattg tttcaaattt
     4861 caacgattaa aacatcaacc caatgatgaa tattataggt ctagaccatc gactgtttgg
     4921 tggaaaaaca ccgattaact acactatgcg gagggtaatg ggcgaagtgt taacgaagtt
     4981 aaacacttcg cccattaccc cttgcgcaaa aattttcagg aaagtataat gttatcagcc
     5041 gataatagtg gaaaggacaa caaaaatgaa aaaaggaatt catcctgatt ataaaaaaac
     5101 cacagtcaaa tgcgtttgcg gtaatgaaat agaaacaggc tctattgaag aaggtatcac
     5161 ggttgaaatt tgcaacaact gccacccgct ttacacagga caacaaaaaa ttcttgactc
     5221 tgaaggaaga gttgaaagat tcaacaaacg ctacggcaaa aagtagtttt tttattaaat
     5281 taattgaaga tatagcccat ttaccaaaaa tgggctatat ttgtaaaaaa acggattaag
     5341 aaacggagag gaaaatgtca aaaaataaac ctgtagtcaa tttaatatca aaacccgaga
     5401 acatgcttaa gaccgtttat acggcatgca gaacatgtta cagtgcggat tatccgatta
     5461 atatttataa cagcacagat gacgaagaaa aaatgttaaa actgatagaa cgggtaattt
     5521 cttcgggaca ttattcaacg attgaacaca ttcagataag ttttgccatt gcaaatgttt
     5581 caagggcatg cactcaccag cttgtaagac atcgccacat gtctttttct caaaaatctc
     5641 aaaggtatgt gaaagaaaaa gggcaatttg attatataat tccgcccaca atagaaaaaa
     5701 atcccgaatt aaaagaaaag ttcgaaaact ttatgggcga gatttcaggt ctttacaatg
     5761 agtttaccga agcggggatt cccgcagaag acgcccgatt tgtgcttcca aatgcggcag
     5821 cttcttctat ggtggcaagc ttaaacttaa gggaaatgat tcaccttgca aatttaaggc
     5881 tttgcacaag agcgcaatat gaaataagaa ttctggtaaa aatgatgtgt gaagaactta
     5941 taaaacaaga gccttggctt aaaccgtatc ttgttcccaa atgcgaaaga cttggttttt
     6001 gcgatgagga taagtcctgc ggcagagtgc aaattaaaaa acaactaatt taatactaag
     6061 aaactaagtt ttggacctct tgtctgtctt cctttggcaa ataacaaatt ctatcggcca
     6121 aaatctgttt aatttcacta tttgcatttt ttgcaagttt tctaaaacaa gccgtccttt
     6181 cttcatcctg caagcaatca atattaaagg caagaacttt tttaagatta gcatctgcat
     6241 ttttaagaag ttgataaaaa taaaataacc tttctttttg aggcaaacac gttatatttt
     6301 caacaatggt cttcttaact ttaatatcgg actcttttag aaattgatca aaataaaagc
     6361 ccctttcttc ttcaggcaag caatatattc tgttaataag agctgcttta gctctattgc
     6421 ttggattttt tgctaatagc ttaaaacaag ctgctgtttc ttttaacggc aaacaatata
     6481 tcccgtaagc aagattttcc acgctgccat tactaggatt ttctaataat tccctaaagc
     6541 aggatgtcct ttcttcttca ggaagattat atattctagc agcaagagcg tttttaactt
     6601 cactatccgc attttctaaa agattatgaa aataacgtgc cctttgatgc tcaggtaaat
     6661 attctatcat agaagaaagt actaatttaa ttcctttatc agtgcttgta aagtattgct
     6721 caaatatttc tttcctttga tttaccggct tttctaaaat atcttttatc aacttcttaa
     6781 tctttttatc tttaatcctt tgacttgaaa taattattct aaatccaccc caatcatact
     6841 gtgtgccgtt tatttcatta tattctttgc caatatcact agaatgcaac ccaacaattt
     6901 caggataaac gaatttatca ctttttggag tatcttcatc aataaattta ttcaccatat
     6961 atcctgcatc tatatctcca aaataaaatc ttatcatctg cgtgttaaaa cctgcttgct
     7021 tttgccaata taatgcgcgg ctttgttcaa tataattccc gtgcatatca tctaaaccct
     7081 tgtcaagatt tttattcaat tttttaaata tttttattat ataatctgcc cctaaaatat
     7141 tattcaattt gtatgcctta ccataagtac cttctccaag aaattccatt ttgaagtttt
     7201 cctgattttt aacaatcccc gcttgaatta aagctgtttt aattcgctct tgcataaatt
     7261 gttcattttc actttctcta aactcattga tgattggttt taagtcatta aacagttctt
     7321 taataacttt ttctctgttt tcttttggta tttttgcaat ccacgccggc ggaagattac
     7381 ccacaattcc atttgattta ggataaattt cacttaattc ttcaatattt attgtatatt
     7441 catatgtatt tttgctcaaa actctattta atccggtaaa attaaccaaa tctttattat
     7501 tatttaattg catcgcagag caacaagaat tcgatttttg ctctaaaaca gaaggcttaa
     7561 gactatcttg ttttggtttt atattaatgg atttgaaggt atttgctctg atcgcaatat
     7621 tgttcataga caacgacact actcctttct tttgtaaagt aaaaacaaat aaaaaaatgt
     7681 cctaaattct aatgaaatta atactttgtt acattaaaaa caggctaaaa aactatataa
     7741 aaattatttt cttgatataa ttaaataaac acttttggga gaataaacaa acaatgaaag
     7801 acatgttaga caaaatttta cagatagaac aaaagtatat ggaattgggg gaaactcttt
     7861 ccaagcccga agtcatacag gattataaca agttcaaaga actttcaaaa caaagaaaag
     7921 cgatggaaga aaccgtcgaa ctttatcatg cctggaaaaa agcgacagat gcggtaaaag
     7981 aagcgcaaga attgatttgg gcagaaaaag acgaagaaat gaaagagttt ttgaaagccg
     8041 aaatggaaga aaatgaagca aaaattcctc aatacgaaga gcaaatgaaa gttttgttgc
     8101 ttccgaaaga ccctatggat gacaaggata ttatgcttga aatcagaggc ggagcaggcg
     8161 gcgacgaagc aaacattttt gcaggcgacc ttttcagaat gtatataaaa tattttgata
     8221 aacaaggctg gcaggcacag cttttgagca aaaccgattg tgaagcgggc gggttttctg
     8281 aagttatagt ttctgttaag ggagaaaatg tttattcaaa attaaaatat gaatccggag
     8341 ttcacagggt tcaaagagtt cctgcaacag aatctcaggg cagagttcac acttcaactg
     8401 ctacagttgc ggttatgcct gaaattgacg acgttgaagt cgaattaaga caagaagatt
     8461 tggaaatctc tacagcacgc tcaggaggtg ccggcggaca aaatgtaaat aaagttgaaa
     8521 ctgcagttcg agttgtacat aaaccgacgg gattaatggt tttctgcacg gaagaacgct
     8581 ctcaattaca aaacaaagaa agagccctgc agattatcaa aaccagactt tatgatatgg
     8641 aagttcaaaa acaaatgcag gaagttacgg atttacgccg ctcacaggtg ggaacaggcg
     8701 acagaagcga gaggataaga acttataatt ttcctcaagg aaggcttact gaccacagaa
     8761 taaatcagaa ccttaacgtt tggactgtaa ttgaagggga gctggacgaa ttaataaaac
     8821 ttttaataga atacgaccaa aagttaaaac ttgaaaaact tgctaagggg taagggtaaa
     8881 tataattatt taaacattga gaggtataat tatggaagat gataaatgtg aaggttgtag
     8941 atatttatta gagaaacatt ttcaatatta tcaaactcaa tatattgaaa gttcaaagta
     9001 tacatctacc attataacag ttgcatatgc ttcattaata tcctttttga tacttacata
     9061 caattatatt aacgattttg ctttattggt aattcttggt tttcttatta tttcaatttt
     9121 attgtttgta attcaattga tttggaatat ggagttaatg agaaaaagta cagaaataat
     9181 gaatgaacta tggcaagaat attggcaaga aaaaattaag cttaatgatc taattcaagg
     9241 aatttataca aaaaatagtg aggcaaaaaa ggaatttaat cgctggaata actttttatt
     9301 tcagtggact ttgatcacag gaatactctc ggcactagtt ttattaatct gtatatttac
     9361 aacattaata tctcctgtca tgctgaattc atttcagcat ctatccagcg tacgctgatg
     9421 agatcctgaa acaagttcag gatgacagtg gttttggatg attaattttg atttggcggg
     9481 gtttcagggg tgtcccctga cgctttggca ttttgttcgc ggtttttttc ttttctttcc
     9541 gtctggtttc ttttcttttt ttgcatcgca acaaaaaaag aaaagaaatg agacaacaaa
     9601 aaaataggtt tataaaaaat ctaccaaaaa atcccaaaaa taaaaaaata ttaatattaa
     9661 aactttttgg attgcttcgt cagaaacgaa tttcttcctc gcaacgacga gcggggcatt
     9721 aaaattaatc aaaatacatt taccccctcg caacgacaaa gcagacagtc attaagccac
     9781 tttaagttta taacccaatt cttttaaaat cttcattacc gtgctcattt gaggctgaac
     9841 ttcaccttta aaaatagcgt aaagattgct tcttgatata cctgtctttt ttgatattcc
     9901 tgaaatactg tcttttgctt ttattgcata ttcaagtgag tgataaaacg ctgaaaaatc
     9961 attatcttcc aaatattctt caagacttac ggttaaccaa tcacgtatat catcttctgt
    10021 tttcaaacta tctgatatta aattttctaa ttttcctgta tttttaattt ttttagtcat
    10081 tttcttgtct ccattgttca agtaattctt ttgcttttac aatatctttt gattgagttg
    10141 atttattccc gccatttatt aaaagtaaaa ttacattatt aatttctgta taatatattc
    10201 tatagcccgg accaaacata aaccttaatt ctgatatttc atcgtcaatt tttttgaaat
    10261 cccccaaatt tccttcttca atccttataa gcctttgcaa aatccttttg gcaacaattt
    10321 tatctagaga ttttagccaa tcctttaaag gttcttttcg attgagtttt aaatataata
    10381 ctttacgttt gtccataatt atagtgtact atatatagta cacaaagtca agtgtaaaaa
    10441 tattgtgtta tactttcttt atgaaaaaag aagtattgcg caaatgtatc ggatgttttg
    10501 agagcaaaaa ccgcgacgga atgattaaaa tcacccgcca acacagttca aatgaaatat
    10561 ttattaaccc taattcaaaa atatttggca gatccgtata tctttgctat aataattctt
    10621 gtatagagaa ctctttgaaa aaaaacaaat tggcaaaatt cttaaaagcg aattttaacg
    10681 aggaattcaa agaaaggtta agaactatga ttagttaatg acagatagga aaatatgcaa
    10741 atgaccttag acaccatgag agtaaatgaa ctggcgaaag agcttaatct cacaaacaaa
    10801 gagattatag acaaatttgc acaactatcg attgttatca aatcacattc aagcaccgta
    10861 acaggcgacc agattaaaaa attaaaagat tttatagaag cgggttcaaa agttgaagtc
    10921 aaaaaaccaa aagccttcat tgtaaaaaaa gttagaacag aagcccctgc cgaacctcca
    10981 aaagaggaag aaaaacctat cgtaaaagca gaaattgaaa agcccaagat agaagttgta
    11041 aaaactgttc caaacagact tgaaatagta agaaaagccc ctgctcaaag ggaatttcca
    11101 agcagaccgt ctgcgcctat tcacaaaaaa cctcaggaaa aaattgaaaa atccacccaa
    11161 acaaaagatg aagccgaaac atcaacagag atttcaaccg agaaaaaaac tgacggtgct
    11221 gttgtcgaaa aaaccgaaag aagacagcca atccagcgtc acataatttc tcaggacatt
    11281 tacaacaaca agggcggaat aaaaagaaaa gtagaaaaaa caaagcaaaa agtttttaca
    11341 acaaaagaag aagaacaaga gcgaatttca ctagaaaaag ctgccgctca aaaacataaa
    11401 aaaagaagtc aacgcgaaga agaagtcgaa caagtaacaa agcttgttgt aaatcagcct
    11461 atgacaataa gcgaacttgc tgtaaaaatt caaaaaacac ctgcagaaat tgttaaattt
    11521 cttatgctgc aaggggttat ggcaactgta aatcagctta ttgatgttga aacaatcaaa
    11581 aaagtttgca atgaatttga actggaagtc ttagatgaag atttggaagc ttttgttgaa
    11641 caggaattgg aaaaagaaga gaaagccaaa gcacttcaag ccgttgataa aaaattatta
    11701 aaacccagag ctccggttgt gagcattatg gggcacgttg accatggtaa aaccactttg
    11761 cttgacagta tcagggcttc aaaacacaaa attgtagcaa ccgaagtcgg cggaattacc
    11821 cagtcaatcg gtgcttatac agtttttgtt gacaagaaaa aagttgtatt tattgacacc
    11881 cccggccacg aagcctttac ggaaatgcgt gcccgcggtg caaaatctac cgatatagca
    11941 attcttgttg tagccgcaga tgacggaatt atgcctcaaa caattgaagc gataagccac
    12001 gctaaagcag ctgaaacacc tattattgtt gcggtaaaca aaattgataa agaaggtgca
    12061 aaccctgaca gagttttgca acaattaaca gaacacgggc ttgttcctga agaatggggc
    12121 ggtgaaacca tagtggtaaa agtatccgcg cttaacggaa caggcataga cgaattgctt
    12181 gaatatattc tgcttcttgc agaaatggct gatttaaagg caaaccccaa agcacacgct
    12241 tcaggtgttg ttatcgaagc aaatcttgac aaaggaaaag gtcctgttgc aacgcttttg
    12301 gtgcaaaacg gaactttaag agtaggaaat tgtcttgtag taggaagtgt cggcggaaga
    12361 gtaagagcgc ttctttcaga tgcgggagaa agggttgcaa aagcagagcc ttcaactcct
    12421 gttgaaattt tagggctcac ggaagtgccc caagcaggcg attattttga agttcttgat
    12481 aacgaaaaag caatgaaatc aatcgttgac aaacgcaaag aaaatgaacg ctcaaggagg
    12541 cttgatgcaa tgcttcctgc gcatatcaga aaagaatcgg cagcaggcga cgagggcatc
    12601 actcagctta atttgataat aaaagcaaac actcacggct cagctgaggc tgtagcacaa
    12661 gctgttgcgc agcttgaatc aaaagaaatt gccacaaaaa ttattcacgt cggagtcggc
    12721 gatatatcag aagccgatgt aatgcttgct tctgccagtg gggctataat tttgggattt
    12781 accgtaaagg aagattccaa cgcacttgcc gcagcagaaa aagaaggcgt tgtaatcaaa
    12841 aaatacgata ttatttatca ggttttggaa gatttggaaa aaacaatgct ttcgctcctg
    12901 cagcctgaaa tcaaacatgt tgaaatcggt aaagccgaag tcagacaaat atttaccatc
    12961 ggaaaaacag ctaaaatcgc aggatgttat gttacagaag gcaaaatcca aagaggaaaa
    13021 accgctgttc ttatgcgtga cggtgaagag atattcaaag gcgtaatcga ccaattgaaa
    13081 cgctttaagg atgacgttaa agaggttaaa gaaggctttg aatgcggaat ttcttttgct
    13141 aaattcaacg acattcaaga aggcgatatc atagaagttt ccacaacaga agaaatcgaa
    13201 cgggttagtt tagattaaag tataagatta taagttaatg agtttataac aaaggcggga
    13261 aaattttttg aaaaaatttt cccgcctttg ttaaattatt taggtacgta aaacgcatct
    13321 tacgacttac gccactatat tttaaagttt atagtatgat caacaaaaaa gttttgtcca
    13381 ccgcccaatc catttataag gtcgtcgctt tccgtataat ttttgtataa gtcttttaac
    13441 tgattatgta gtcttctgtc ttcagtcgtt acaaattcat cgccaatttt ttgagctttg
    13501 tcaaaaatag cactatataa tctgtcttta attgaagttc cgccatttgt ccttacatta
    13561 tagccaaaag taccttcagg aatttctttt aactgtagaa ttaatttgtt agcaattctt
    13621 ctaaaagact tgccctcttt taatccggta aaattttgtt ttgattgata atcagcgttg
    13681 attgaattgg tcaacatttt ttatacctct ttctaaaaat taataaacaa taatatctat
    13741 atggttaaaa accaaaaata catttttttg ctagtgattt aatactttgt tacattgtat
    13801 tataaacttg tttttgataa aatcaaagta aaggaataat ttatgacact cagaaatgaa
    13861 cgcgttagaa aagaattaat gagagaaatc gccgatattc tccaaaaaga aatcagaacg
    13921 gcgggaatga catctataat ggatgttgaa gtttctcacg acaattcctt cgcaaaagtt
    13981 tattacagcg tattcggcag cgatgaacaa aaacaaaaaa cgattgaaac aatagaaaaa
    14041 aatacctcca aaataagata cgaaatcggc aaaagaataa gattaagatt aactcccgaa
    14101 cttcgattca tttatgacga ttcaatcgag cgcggaacaa aagttaccga ccttataaat
    14161 aaaatttccc gcggggaaat aaaatagtga cctgttaata ttattgtcgt tgcgaggaat
    14221 gaaatgacga agcaatccaa aaaattttta atgttaactt ttttcttaga ttgcttcgac
    14281 tttttgcaaa agtctcgcaa tgacatgaaa gtgttaattt aatgtttggg tttttaaatg
    14341 tgtataaacc gacaggcatg acctctcatg atgttatttc tgttttgcga aaaatttcag
    14401 gcattaaaca aatcggacac agcggaacgc tcgatccttt tgcgcaaggc gttttgccga
    14461 ttgcaatagg caaagcaacc cgtttaatag aatatttgaa cgatgataaa gaatatctcg
    14521 ccgaaattag tttcggaaaa gccaccgata cgtacgactg tgacggaagg gcgatttctg
    14581 aatgcaattc agaaatcgcc ctgaatgacc ttcaaaaggc actaaaaacc tttgaaggcg
    14641 aaatctttca aactccgcct atttattctg cgataaaagt taaaggcaaa aaactttatg
    14701 aatacgccag aaaagggcaa gaaattgaaa tcgagcccag aaaagttttt attgaaagaa
    14761 tcgaactgaa atcctttgat gaagaaagtc aaaaagcgca ggtcttgatt aaatgctcaa
    14821 aaggaactta tattcgctca atagcgcatg acttgggaca aaatcttaaa gtcggggcac
    14881 acctttcaga gttaatacga acaaaagcag gcgattttac cgttgataaa gctatttttc
    14941 ttgatgaaat taaaacgaaa gaagatattg aaaaaaattt aattaatcct ttaaaaatgc
    15001 ttaatttaac ccaaattatt ttaaacgaaa ttgaatacaa aaaaataact atggggcaat
    15061 tcattgataa taaaaatagt ttacaaaacg acgatttaat tattttgatt tataataatg
    15121 aaataaaagc cgtcgcaaat gttgacggga atttaataaa agtaaagaag gtattggtat
    15181 gaaaaaatta gttttattaa ttgctgcagt attatttttc ggaaacagcg catgggcagg
    15241 atgtgattat acaagcaaat gctcacctgc agcttacgga aacgtttcgc caagttggca
    15301 aagagtttca aaatacaccg gtgcaacttt tttaacagag cagattgcaa attccataat
    15361 aaaaaaagaa ctcaaaaaag ctacaaaaga aaattttaaa gttcacataa aaagttacag
    15421 cgcaaaagat ttagcgcaag gcagattcaa gtcacttcag atttcaggaa aaaatcttga
    15481 aatagaaaac gcttatatta ctttgtttga gcttaaaaca ctgtgcgatt ttaattacgt
    15541 tgaattcgga aaagattatt taaagttcaa agaaaacatg gtaatggatt ttacaatgga
    15601 aatctccgat tttgatctca gaaggacaat gaaatcaagc ggatatcttg atatgctcaa
    15661 taatacaaat atttcagcac tcgggcttac tcttttaaag cttgaaaatg ccgatgttaa
    15721 aatcaaaaac aacaaacttt tctttacgct aaaggtttct tcaaaactgc ttccaatgcc
    15781 ggttgacatg attttgaaag cggatttaaa agtcgaagac ggacaaattg ttcttacaaa
    15841 agtcgatttt gtaaacacat atacaagaat ggatttgagc aaagctgctt atcttttgaa
    15901 tattttaaac ccgcttacat tctctactga tattcttgaa aacaaaaaat caaaactttc
    15961 tgttcaaaat gtcaacatta tcggtgacaa aataataatt aacggacttg tttttattcc
    16021 aaaaaataca aagtaaggag aaaatatgaa aacctggcaa aaaataagct taatacttct
    16081 tgtatttgcg accatagttt acataaaact ggctttcttt accggctcag aagttgcgcc
    16141 tccgactgat gaaacggcac aagaacagga agaagctcaa aaaatgccca aaatttatac
    16201 atatattttc tttgtaggac aaaatgccga caaagaagaa gtctataaag ccgtaaaacg
    16261 cgagtatccg gaagacaccg atgattcaaa acttaaattt gcgctgacta atttgcttaa
    16321 aggaccttcg gctaaagaaa gaacaaaagg cgtttatacg gaaattcctt caggaacaag
    16381 gcttttgtcc atagaagagc ttccaaataa aattattata aatttaagct ccgattttga
    16441 aacgggcgga ggcacggaca gtctttataa aaggctttat cagcttataa aaacagtcaa
    16501 caaaaactcc gacactcctg tttacttgca gcttaacgga aaacaaattg acgtaatagg
    16561 cggcgaaggt cttatgctta accagccttt gaatgaacgt tcactggatt aatcaccatg
    16621 acaaaagacc ttgattatta tctgaactta aattggacat tgattgaagg tcaagatttg
    16681 gacttcaacg gacagcctta tcattttatt gaaataaaag aaatcccaag ctttgccttt
    16741 tgcgccaaaa caatccaaaa agcaagagaa aattataaaa cacaattaaa atggactctt
    16801 caggtcatgc ttgaatcagg ggaaaaaata atcgaacctg gcgaagaatc ggacgaccaa
    16861 atcgattggg aaagtctttg cccgtagtaa agacataaaa aagaggcgcg agcgttccgc
    16921 tcgcgcctct ttttgttctt ggagaatatt tctatccgtt accgaatgtt ttgaaagaac
    16981 tttcaatgtt tttaccgata actttttgta ccgcttcaat ttctgtttgt atagcgttat
    17041 gttcggtatc tgcctgtttg agtttaagct caagagtttt atcttgttgt tggattatag
    17101 ataattttga attaatatct tccatttgtt tttcatattg agctttgtta tattcatatt
    17161 gagtcattgc tgtatcatat tcttgctcat taacattcga tgttgcattc atactaatct
    17221 gggtaccatt acttaatgta attgaaacag gttgatcgcc gctaaaagta actgtaacat
    17281 cttcaagata agcttcatgt tcatattcaa attcttgcgg tatgtaacct tgagtttgat
    17341 catttgcagc aattgcggca tcaatatcgg cttgtgaaaa atatgttata gctccgtttg
    17401 ttgccgtata agaataataa ggaccattac caatctgagc agcatcattt aattgagttt
    17461 ctgcttcgct acccgaaggt acaagaacta aagcaacgcc gcctatactg tatgaaccac
    17521 tgcttccggt aactgaatat tgtgatgaag gaactatcat agtattagta tcttgataag
    17581 tataagatac gttatataat cctcccggtt ttttaaataa gttagttata ttacaatttt
    17641 caccattcat tgtaatactg tattgtgttt gggcatacgc tgaaacactt ggcggagtag
    17701 gcactgacaa gtccaaaagt tgcctattca aagttgctga ctggtttgcc aaattagttc
    17761 tttgttggtt aatttgttgc ccttcgtatt ctacgttgct ttttctagct gttaagctta
    17821 aataccttgc ttgtgatgct gccataccca tagttttcgt gcctccattt tttaaatatt
    17881 tttcaatatt tatctctaac tatgtattta tcgtactttt taattaaaaa ctttaattta
    17941 tccacttttt tgttacaaat ttgtaatatt ttttaaatca tttttttgat aaaatataag
    18001 aaagaggatg tgaaaaaatt caaaaaatga caattttcgc acgctcaaaa gaggacaaaa
    18061 aatgttta
//
LOCUS       DAOQAU010000034        16973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_3577_length_16973_cov_7.783485, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000034 DAOQAU010000000
VERSION     DAOQAU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 16973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16973
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3577_length_16973_cov_7.783485"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKI94_05950"
     CDS             <1..752
                     /locus_tag="PKI94_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW26318.1"
                     /translation="NKILKRIFGFTLAEVLITLTIIGVVAALTVPNLYAYYQKQQYVT
                     ELKKVYTEISQALKLMMADEGVNKLSTSNTLTAEEGEGDFDEAVLNRAGGFLKKYFKV
                     IKDCGMEDPNSCFAQDSKGTDGTNFTPGNPHYCVIIASGASICMRPANVAKSGTIIVD
                     VNGLKKPNIAGRDIFSMAFFYDGSIDGFQINPQCRQGKRTWNGTGALCGGYGNNASEA
                     RENYFSDGCLINGYGSSCFGKILNDNWKMDY"
     gene            complement(811..1881)
                     /locus_tag="PKI94_05955"
     CDS             complement(811..1881)
                     /locus_tag="PKI94_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNW26319.1"
                     /translation="MQLDYSKLFDISRDLYALGSYNNKLGRAFIPLRYFFELTYLCNL
                     NCPYCYVGKDRKKDELTTQEWFNIIEQIPWYSFVTLVGGEPLLRKDFIEILEKTAKKT
                     FGKLNVVTNGILINDEIIDAFIKTKMMLLSVSLDGYGKTHDKNRGQEGIFDTIIGNLE
                     KLNSKKKNKPMVDIKTIVLEDNLDDLPKLYKLCEDMNFDFLSIAFLRNNDLKQNSILR
                     QSFGEEFYLQKYPIKPYFDMEHFKEVYRKIEAMKGKTQIRFSPKFSDKNPLEDIEKFF
                     NCGDKPIQEIYEPCKYPWSNMMINPQGDVYPCLSLKVGNIREKSLKECFNEPKYCCFR
                     KNLKISKVFEACQMCCELCIKK"
     gene            complement(1935..2975)
                     /gene="trpD"
                     /locus_tag="PKI94_05960"
     CDS             complement(1935..2975)
                     /gene="trpD"
                     /locus_tag="PKI94_05960"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNW26320.1"
                     /translation="MKGIIKKITAKEELSFEEIEQIVVKIANQEVSEAQIGAFLVAIT
                     EKGVSKDEFFAFASAMRNFSNRVILDKFVVDSCGTGADFSKSINVSTASAIVASASGA
                     NVLKQTNSSITSACGSSDFLKALDINIARIPQDAKAKFEKNGIAFVHSPYFNDFARVN
                     NPIRQQVGIKTIFNYLGPMINPAFPQAQLLGVSSLEMCEKMVYALDKLGTQKALVVNG
                     LNPNIDEISICSKTAVWELNNRAISYYEIAPEDFGFKTTELSEIQGGRGEENAKIIQA
                     IFKGEIKGAKRDIILMNAGAMIYLAGLAKDMFEGAKIAQNVIESGLAQKKLLELQERI
                     PLTHFSNARTSH"
     gene            3041..3571
                     /gene="rimI"
                     /locus_tag="PKI94_05965"
     CDS             3041..3571
                     /gene="rimI"
                     /locus_tag="PKI94_05965"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNW26321.1"
                     /translation="MQEQLKIKILPMQKSDVEEVMKIEEQAYGEHHWSKDSFYGELSN
                     ELAHYYSAFDLAGNLLGYAGTWRILDEAHITTIAVKPELRRKKIGEALLIRAIEDFYK
                     NEIKYVTLEVRVSNIPAIKLYEKYGFKSLGERKGYYQNNNEDALIMWTENIFWDKFKL
                     RYEQNVKNLTEFITVE"
     gene            3620..4300
                     /locus_tag="PKI94_05970"
     CDS             3620..4300
                     /locus_tag="PKI94_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNW26322.1"
                     /translation="MTKRLVKQLEKIQYANQPLKLNITTIILTVICTFFIVIATFTQF
                     DFNHYVLPFDWFAYLGKNFKDPAVYSHFIKHYRYIPQVPMIMFIAAFLGRRFGIASVI
                     LYIVAGLFIAPVFAIGGGLRYVLEYGFGYILAYIPAVFFAGSITRSGLTYINMLQATL
                     VGVCTIHLMGIFYMLFVATIQRETSALILGWISAQSGVKIIYDIVFGFFAMIIAKYLK
                     KWLWFAMS"
     gene            complement(4462..4932)
                     /gene="rpsG"
                     /locus_tag="PKI94_05975"
     CDS             complement(4462..4932)
                     /gene="rpsG"
                     /locus_tag="PKI94_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNW26323.1"
                     /translation="MSRRSKPTKRIPPADPIYNSVDVSKFINRIMRRGKKSLAEKIFY
                     STLENIKEKTNEKPMEIFQKAVTNATPLLEVKARRIGGSTYQVPLEVKPDRGMALAFS
                     WIITAAKSRNGKSFIDKLTNEIMDAANGSGTACKKREDTHKMAEANKAFAHYRY"
     gene            complement(5109..5492)
                     /gene="rpsL"
                     /locus_tag="PKI94_05980"
     CDS             complement(5109..5492)
                     /gene="rpsL"
                     /locus_tag="PKI94_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNW26324.1"
                     /translation="MPTINQLVRKERKNLTDKTKSPALMECPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTSYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DTAGVANRSQARSKYGTKREKAKKS"
     gene            5668..6354
                     /gene="ubiE"
                     /locus_tag="PKI94_05985"
     CDS             5668..6354
                     /gene="ubiE"
                     /locus_tag="PKI94_05985"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNW26325.1"
                     /translation="MKNGVNIKEMFDKISAKYDKLNEIMTLGLNSSVKIKAIKNIPLK
                     SDVKVLDVCCGTGDIAIHIAKNIAVKGEVTGVDFSSKMLEIAKNKAQNLKNITFFEAD
                     ALNLPFAENEFDACFISFGLRNLEDLKNGLEEMKRVVKPGGYVVNLDTGKPKGIFKFF
                     HKIYFHWAVPVMGKIFGGNYEPYKYLTTSAKDFPSSDELVELFKQIGLKNVSKHEFLF
                     GAISQQFGQV"
     gene            complement(6351..7337)
                     /locus_tag="PKI94_05990"
     CDS             complement(6351..7337)
                     /locus_tag="PKI94_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNW26326.1"
                     /translation="MENFNRIIFWLKAARVHTMPMSFMSWLVVFCWSYSIGGDFFYGI
                     LALIGIMSAHMGVNLVDDYFDYKEELKEQKFQNDPQSITMQKGKCKYMIEGKATLEQT
                     FRALSVFFAIAIFIGIFLAFKCGWQVLLIAFVASLFCAFYPFLTYRGLGEIAVGMTFA
                     PLLFMGTSFVMLGGFSSDILLLSISTGLLTVGLLHTHALMDYDFDVKNNKKTLCTLLK
                     SKEKALVALGIMMAGAYLNIILGIFTNKFPASTAITLISAPLAVVLYKSMSLTIQDPN
                     TSLPKKFWMGPMEHWEEIKANNAHNFMLNFYLSRNIMMFFTILLCISLLTGY"
     gene            complement(7405..8790)
                     /locus_tag="PKI94_05995"
     CDS             complement(7405..8790)
                     /locus_tag="PKI94_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW26327.1"
                     /translation="MLVNRINNLNNPTFGYDKKLNNKVKATLEKNYTPFNKTLLATNE
                     FCNQLEDTIIEREKNLAPIYEIDNLVRMLASFKYVFAQMMEALFPKLNYVATEAEGYL
                     NNTAVKKDNQDWRINIASCLYDIYEPTEENLEIKLLKQEFDEKYTKVMNIVQEELTGS
                     MNPNSKKEEKSLISEFKPNEYSPKGFVSIGGMLDLLTDLKEKIIDPIKDPRQAQIDRL
                     EYGKKFPKAILFYGPPGCGKTFITEALAIQADIPLFKFKVSELGSSYINKTSENYENA
                     FKQVEEHAKKIGKPCFLFIDEIDGVTKNRDNSDHAEDLKQMSTLLNLIETARARNIIV
                     IGATNKYDLMDEAIRQRFDGHFHIGLPDENTRKEVLEKALEERQKAQNLLSSSDDICA
                     VAKAFEGFSNRTIVDITDKAAIKALKDNRRDITKQDFFDIIDESSDLKVEDPSFYKHK
                     KTKEIGFKHEN"
     gene            complement(8886..9395)
                     /locus_tag="PKI94_06000"
     CDS             complement(8886..9395)
                     /locus_tag="PKI94_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNW26328.1"
                     /translation="MKGNVLNGKAYKFGDNISTDHIAPGRLFHLRSNLEEFAKHVLED
                     ADPEFANKMQKGDFVVAGNNFGLGSSREHAPQIIKIAGVQAVLAKSFARIFYRNAINI
                     GLLLIECDTDLINACDELELDLKSGIVKNLTQNSQIKFTPLPEVMIKLLEDGGLIEHI
                     KKHGDFNLV"
     gene            9557..10075
                     /locus_tag="PKI94_06005"
     CDS             9557..10075
                     /locus_tag="PKI94_06005"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNW26329.1"
                     /translation="MFNRKCKITFIAHGATIHSEENRVSDKENYPPINEAGEEEMAKI
                     CEWLKKRAIKTDKIYTSPALRTIQSAKIISKALKIDFEILETLTSKKNEEISDFTKRI
                     STVIKQIVEENIGKRIIIVTYPEVIQAAISSAIKLGAKNQTNIYIKTGSASQISYFEN
                     WSSLVYSGCVPL"
     gene            complement(10076..10888)
                     /gene="rsmI"
                     /locus_tag="PKI94_06010"
     CDS             complement(10076..10888)
                     /gene="rsmI"
                     /locus_tag="PKI94_06010"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNW26330.1"
                     /translation="MKKEFQLYIVPTPIGNIEDVTLRAIDILKSVDIIACEDSRITQK
                     LLYRYDIKTKLISYHKFNEKQRVNLLLKLLKEGKKIALVSDAGTPLISDPGSILISEL
                     GLNGINFTALPGACAVPTFLSQVPRDGEDFAFIGFLPKTKGQIFAILQKFAHTNLVFY
                     ESPNRLLKTLEGINEFNPKAKVAVGRELTKVFEEIVIDKVQNVIEYYKANPLKGEIVV
                     MVYKKESTEFCQTEILEKIELLKRKKFGAKDISTILAELYDLNKNDIYKIII"
     gene            10967..12733
                     /locus_tag="PKI94_06015"
     CDS             10967..12733
                     /locus_tag="PKI94_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26331.1"
                     /translation="MRLGKKYLITLFILFILVVIFYISKKTVEFVYVNPNVNTQVLPF
                     ISPVIKQIDDGVNFSVLNCNLDKQEMKILYQNAYGSGFLKHNPIPNDLDYSVGIHLGK
                     YFYDGKNALEIAKDIDNKMSCFQINFYDYISNNKNQGFLTELDTLGSIIHFDKKSSEN
                     IKFIEQSLPEIFKGREYIVYLKKTFPDKPGFVLEYPFVLKPNEILIEDYAPVQLFAKG
                     VRYNKKTQDFLREITIVVDFYADIEDVKTKKTKTVEIVAESFAGQRLQLSRRFFPPIV
                     FVGENSARYLKNLDYLKEDEKYLEYRLFNFKRHLQEFSNLNEFNDRPVKMLKRILQCT
                     EIISPALDEKTKDEIYENIYQNLKNPNVIILNDYSTALGNLIQITQMPKLYAKALNSG
                     DLQKLVESMREDLNKLKPTNSQDVKELIEFQDYIEKNILTVKSNEDLVFFSQNLLLKM
                     DRYNKNIINFFNKNVTNLKQIISYIDVFGKIYKDSGFYKIELYWLDRNTLGIVKDDFT
                     KNIAQNELKEFARQNKLADVNYKLISKVQAPKLSVRYETWVKYHSTAEEEKNWQLLKS
                     QLLNDKKNFKIRRKIFFIKKYY"
     gene            12785..13357
                     /locus_tag="PKI94_06020"
     CDS             12785..13357
                     /locus_tag="PKI94_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNW26332.1"
                     /translation="MLVQNINLLNIRNRYRPNTPQKINFKGKDGLPDNFGIVDEGKLY
                     RSANILSKSEFEKLKEKGVTFIVDLRDPAEDGHSMLHEKSRAYEFGLEHISFPDNEFC
                     RDMEKLYKITEDIYKKIQKGGVGLIHCTMGEQRTGWVAAAYKFLYKNADFKTVVDDYN
                     RYCPNADSISIYMLDFIKEVVDAKKLLKSI"
     gene            complement(13354..14799)
                     /locus_tag="PKI94_06025"
     CDS             complement(13354..14799)
                     /locus_tag="PKI94_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNW26333.1"
                     /translation="MEKALSKNHLIILDTFFKYLPSRFFIILNSLIIIPFLAHVISAR
                     EMGIFQLCIGILNLVCTCSTDWIAKSALRFWHKYKRKKMTDKFLSNVVFLTFLSYILI
                     FVLYFCLSGFIIQKFSIDRISLLLTLFLVIPCGIRQFLYQMLRILNKPFMYTFSIIFY
                     QLSLLVLFLFFSNLYSDIYALLIAMTVSIFVIDVYIIKQIGLEERLNIKPDILILKES
                     LKYSLPLIGTNVSIWAVLNINKFIFQYNNMFFETAVTGICWFFTTNILAPIFSTLLFS
                     VFPTIIKQFEKKRLINEFVTSTLQLYFVIFIPFVGIFCFFSKEIAKFILSEKYFAGHI
                     LLPFFAVSIFAHEFLKLMNIKYHLRNKTYIEMGLSIFVGIVAVLLNIFLLPKFNLAAA
                     GFIMIFCVFLLIVLNSLINFKSLDYIESSKILKTGILATILMPVCFLISNFIFEKLIH
                     EPLFIIAKVIISCTLYYILMWQFKSKILDQS"
     gene            complement(14870..15169)
                     /locus_tag="PKI94_06030"
     CDS             complement(14870..15169)
                     /locus_tag="PKI94_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010893244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNW26334.1"
                     /translation="MEMFNPPHPGEILLEEYIKPLNFTVSAFALNLGTSRKNLSEIVN
                     EKVGISPEMALKLAKVLGTSPEIWLRLQLKYDLWQAKQKVNLDNLQVISAKRIIA"
     gene            complement(15180..15458)
                     /locus_tag="PKI94_06035"
     CDS             complement(15180..15458)
                     /locus_tag="PKI94_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNW26335.1"
                     /translation="MIKSFKHKGLENFFKTGSIKGIIPEHAKKIRRILAILDEISDLA
                     EINFDGYNLHQLKGNMKGLWAMRVNGNWRITFEFKNQDVYIVDYKDYN"
     gene            15573..16370
                     /gene="tpiA"
                     /locus_tag="PKI94_06040"
     CDS             15573..16370
                     /gene="tpiA"
                     /locus_tag="PKI94_06040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003643975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNW26336.1"
                     /translation="MRKPIIAGNWKMNKLQAEAKELFLGIAYFAERFTKQQLPEIVLA
                     PTFTSLAAVESMRCDKCRGLVSIAAQNCHWEKSGAYTGEISVDMIKDAGCEYVIIGHS
                     ERRQYFSETDEMINKKAKAILEGGLIPIICCGESLEQREAGITDAHITKQIKAALEGL
                     SEEEIARSVIAYEPIWAIGTGKTCDSVEANRVIAMIRNVVKEVSTEKAGDAIRILYGG
                     SVTPASIEEQMSQSDIDGALVGGASLKIDSFSEIIANTMKATQFLTT"
     gene            16515..16766
                     /locus_tag="PKI94_06045"
     CDS             16515..16766
                     /locus_tag="PKI94_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26337.1"
                     /translation="MAKQFSGTPQIKTRTALLIFIKGSSAPVVLYLENPQAAYEEIQK
                     LIKAPTAALIEKEAVGPIKKVSLQVSQISAVALQEEQYV"
BASE COUNT         5610 a         2762 c         3004 g         5597 t
ORIGIN      
        1 aaaataaaat attaaaaagg atttttgggt ttacattggc tgaagttttg ataacattaa
       61 caattatagg tgttgttgcg gctcttacag ttcctaattt atatgcatat tatcaaaagc
      121 agcagtatgt gaccgagtta aaaaaagttt atacagaaat ttcccaagcc ctcaaattaa
      181 tgatggcaga tgaaggtgta aataagcttt caacttcaaa tactttaaca gctgaagaag
      241 gtgaaggtga ttttgatgaa gcggttttaa acagagccgg cgggtttttg aaaaaatatt
      301 ttaaagtaat caaagattgc ggcatggaag atcccaactc gtgctttgct caagactcaa
      361 aagggacaga tggaactaat tttactcctg gaaatcctca ttattgtgta attatagcaa
      421 gcggggcaag tatttgcatg cgccctgcta atgtagctaa atcagggaca ataattgtgg
      481 atgtgaatgg acttaaaaaa ccaaacatag cgggtagaga tattttttct atggcatttt
      541 tttatgacgg gtcaattgac ggatttcaaa taaatccgca gtgtagacaa gggaaaagga
      601 catggaatgg tacgggtgct ctttgcggtg gatatggaaa taacgcctct gaagctcggg
      661 agaactattt ttctgacggc tgtttaatta acggttatgg ttcaagttgt tttggtaaaa
      721 tcttaaacga taactggaag atggattatt aaattttttg tctttgcgag gagtgcgtga
      781 gcacgacgcg acaatccaaa ataatttaca tcatttttta atacacaact cgcagcacat
      841 ctggcaggct tcaaatactt ttgaaatctt taaatttttc ctgaaacaac aatattttgg
      901 ctcgttaaaa cactctttca aacttttttc tctgatattt cccactttca aagacaggca
      961 tgggtaaaca tctccctgag ggtttatcat catattagac caaggatact tgcaaggttc
     1021 atagatttct tgtataggtt tgtctccgca gttaaaaaat ttttcaatat cttccagtgg
     1081 gtttttatca ctaaattttg gcgaaaagcg gatttgggtt ttgcctttca tagcctcaat
     1141 ttttctgtaa acttctttaa aatgttccat atcaaaataa ggctttatag gatatttctg
     1201 caggtaaaat tcttccccga aactttgtct taaaattgaa ttttgtttta agtcattgtt
     1261 tctcaaaaaa gcaattgaaa gaaaatcaaa gttcatatct tcacaaagtt tataaagctt
     1321 tggcaggtca tctaaattgt cttcaagcac aatggttttt atatcaacca taggtttgtt
     1381 ttttttcttt gaattaagtt tttctaaatt gcctataatt gtgtcaaaaa tcccttcttg
     1441 ccctctgttt ttgtcgtggg ttttcccgta accgtctaaa gaaacagata aaagcatcat
     1501 ttttgttttt ataaaagcgt caataatttc atcgttgatt aaaattccgt ttgtaacaac
     1561 gtttaatttg ccgaaagttt tttttgctgt tttttctaaa atttcaataa aatcttttct
     1621 aagcaaaggc tctccgccca ccaaagttac aaaagaatac caagggattt gctcaattat
     1681 attaaaccat tcctgcgttg taagctcatc cttttttctg tcctttccca cataacaata
     1741 agggcagttt aaattgcata aataagtcag ttcaaaaaag tatctcaaag gaataaaagc
     1801 cctgcccaat ttgttgttgt aagagccaag cgcgtacaaa tcccttgaaa tatcaaaaag
     1861 ttttgaataa tctagttgca tgtaatgtcc tttggtttgt ttttccctcg ccccttgggg
     1921 gagagggtga tttttcaatg cgaggtacga gcattagaaa aatgggtgag gggtatcctt
     1981 tcttgtaatt caagcaattt tttctgagcc agtccgcttt cgattacatt ttgagctatt
     2041 tttgcaccct caaacatgtc ttttgcaagc cccgcaagat aaatcatagc acctgcgttc
     2101 atcaggatta tatcacgttt tgcgccttta atttcacctt taaaaatcgc ctgaattatt
     2161 tttgcgtttt cttcacccct gcctccttga atttctgaga gctcagttgt tttgaagccg
     2221 aaatcttcgg gcgcgatttc gtaatacgaa atcgcgcgat tgttcaattc ccaaacggcg
     2281 gtttttgaac agatgcttat ttcatcgatg tttgggttaa gcccgtttac gacaagtgct
     2341 ttttgtgttc cgagtttatc aagagcatag accatttttt cgcacatttc aagcgaggaa
     2401 acgccaagaa gttgggcttg cggaaaggca ggatttatca tcggcccgag gtaattaaaa
     2461 attgttttga ttccgacctg ctgtcttata gggttattta cacgtgcaaa atcgttgaaa
     2521 taaggtgaat gaacaaaagc tatgccgttt ttttcaaatt ttgcctttgc atcctgcgga
     2581 attcttgcaa tatttatatc aagtgctttc aagaaatcac tgcttccgca agcacttgtg
     2641 atagatgaat ttgtttgttt aagtacgtta gctccgcttg cgcttgcgac aatagcactt
     2701 gcagtcgaga catttatcga ttttgaaaaa tcagcacctg ttccgcagct gtcaactaca
     2761 aatttatcca agattactct gttggaaaaa tttctcatgg cagaggcaaa agcaaaaaat
     2821 tcgtctttgc tgacaccttt ttcggtaatt gctaccaaaa aagctcctat ctgggcttcg
     2881 gatacttcct gattggcaat tttaaccaca atctgttcta tttcttcaaa acttagttct
     2941 tcttttgccg ttattttttt aattattccc ttcactcttc actcctcata cttcatttct
     3001 caattatccc ataaaaagca atttatgtta aaataatttt atgcaagaac agttaaaaat
     3061 aaaaattctt cctatgcaaa aaagcgatgt ggaagaagtt atgaaaatcg aagaacaagc
     3121 ctacggagag catcactggt caaaagattc tttttacggg gaattatcca atgaacttgc
     3181 acattattac agtgcttttg atttggcggg gaatttgctc ggttatgccg gaacctggcg
     3241 gattttagac gaggcgcata ttacaaccat tgcggtcaag ccagaactca ggcgtaaaaa
     3301 aatcggcgaa gctcttttga taagagcgat tgaagatttt tataaaaacg agataaagta
     3361 tgtgacactt gaagtcaggg tgagcaacat tcctgcaata aagctttatg aaaagtacgg
     3421 gttcaagtct ttgggggagc gaaaaggtta ttaccagaac aataatgaag atgccttaat
     3481 aatgtggaca gaaaatatat tttgggataa atttaaatta agatatgagc aaaatgttaa
     3541 aaatttaaca gaatttataa ctgttgaata atttttctta ttttcttatt cacctattta
     3601 cttattcact cgagggaaaa tgacaaaaag attagtcaaa cagcttgaaa aaattcaata
     3661 tgcaaatcag ccgttaaaat taaacatcac aacgataatt ttaactgtaa tttgtacctt
     3721 ttttatagta attgcaactt ttacccagtt tgattttaac cattatgttt taccgtttga
     3781 ctggtttgct tatttgggca aaaattttaa agaccctgcg gtttattctc attttataaa
     3841 gcattacaga tacattccgc aagttcctat gataatgttt atcgcagcat ttttgggaag
     3901 acgattcggg atagcttctg taattttgta tattgtagcc ggacttttta tagcaccggt
     3961 ctttgcaata ggaggcggtt tgagatatgt cttggaatac ggattcggct acattcttgc
     4021 atatattccc gcagtgtttt ttgcaggctc gattacaaga agcggactta cttatataaa
     4081 catgcttcag gcaactctgg tgggggtttg cacgatacat ttgatgggaa ttttttatat
     4141 gctttttgtt gcgacaatcc aaagagaaac atcagctttg attttgggct ggattagtgc
     4201 tcaaagcggg gttaaaataa tttatgacat agttttcgga ttttttgcaa tgattatcgc
     4261 caaatatttg aaaaaatggc tctggtttgc aatgagctag gaaatagtaa atagtaaatg
     4321 gtaaatagaa atgttaaaac aggggacaag aggcgaagat gccaagaggc gtagtaggat
     4381 agcccacgca tccacgcatc tactcatctt atcttcttat aaactattca acttatcgac
     4441 tattcgactt atcaactaat attaatatct gtaatgagca aaagctttgt ttgcttcagc
     4501 cattttgtgg gtgtcttcac gttttttgca ggctgtaccg gaaccgtttg cggcatccat
     4561 tatttcgttt gtcagcttgt caataaaaga tttgccgttt ctgcttttag ctgctgttat
     4621 aatccaagaa aatgcaagag ccatacctct gtcgggctta acttccagcg gaacctgata
     4681 agtagagccg ccgattcttc ttgctttaac ttccaaaagg ggagttgcgt ttgtaaccgc
     4741 tttttggaaa atttccatcg gtttttcgtt tgttttttct ttgatgtttt ctaaagtaga
     4801 gtagaaaatt ttttcagcca gagatttttt acctctgcgc ataattctgt taataaattt
     4861 tgaaacatcc acgctgttat aaataggatc agccggtgga attctttttg tgggttttga
     4921 acgtcttgac atttttacca ttacacgaaa ccttcgcggt ttcgcccttt ctattcttga
     4981 gtattacttc ttatgtcata ccgaacaatc tgttcgggac ttaattttgt tgggttagaa
     5041 aacccaacct acatattaat aaagtaaata gtgaatggat ttttctattt actatttact
     5101 atttaccatt aacttttttt agctttttct ctttttgttc cgtatttaga acgtgcttga
     5161 gatctgtttg caacacctgc tgtgtctaaa gttcctctta cgatgtggta tcttacaccg
     5221 ggaaggtctt taacccttcc gcccctgata agaacaacgg agtgttcctg aaggttgtgt
     5281 ccgattccgg gaatataaga agttacttca aacccgtttg taagtcttac ccttgcaact
     5341 tttctcaaag ctgagttagg ttttttaggc gttgttgtat agactcttgt gcaaacacct
     5401 cttctttgag ggcattccat caaagcaggt gactttgttt tgtctgtcaa atttttacgt
     5461 tctttacgca caagttgatt aatagttggc attaattttc tcctttttgt tttctaacaa
     5521 agggccatag caaaatgcca ccctcggcta gagttgtatt cctatctaac aacaaccaaa
     5581 agcctttgtc aaccatgaaa gctttgcttt atgcttaaac tttaagtatt ttaacattta
     5641 tgtaataata tttttcaggc ggtgttcatg aaaaacggcg taaatataaa agaaatgttt
     5701 gataaaattt ctgcaaagta tgataaatta aacgaaatta tgactttggg gctaaattca
     5761 agcgttaaaa taaaagcaat aaaaaacatc cctttaaaat ctgatgttaa agttttggat
     5821 gtctgttgcg ggacaggcga tattgcaatt catattgcaa aaaacatcgc tgtaaaaggt
     5881 gaagttacag gggtggattt ttcttctaaa atgcttgaaa ttgcaaaaaa taaagcccaa
     5941 aaccttaaaa acataacttt ttttgaagca gatgcgctaa atcttccttt tgcagaaaac
     6001 gaatttgacg catgttttat aagcttcggg ctcagaaatt tggaagattt aaaaaatggg
     6061 cttgaggaaa tgaaacgggt ggtaaaaccc ggcggctatg tcgtaaacct tgataccgga
     6121 aagcccaaag ggatttttaa attttttcac aaaatttatt ttcactgggc agtgcctgtt
     6181 atgggtaaaa tttttggcgg aaattatgag ccttataaat atctcacaac ttctgcaaag
     6241 gattttcctt catccgatga attggttgaa ctctttaagc aaatcgggct taaaaatgtt
     6301 tcaaaacatg aatttctttt cggtgcaatt tctcagcaat tcggacaggt ttaatagcct
     6361 gttaaaaggc ttatgcataa taaaattgtg aaaaacatca taatatttct tgaaaggtaa
     6421 aaattgagca taaaattgtg agcgttgttt gctttaattt cttcccagtg ttccatcggt
     6481 cccatccaga atttttttgg caaagaggtg ttagggtctt gtattgtaag gctcattgat
     6541 ttatacaata caactgcgag aggagctgaa atcaatgtta tagctgtaga tgcggggaat
     6601 ttatttgtaa atattcctaa aattatattc aaataagcac ctgccatcat aatgccaagt
     6661 gcaacaaggg ctttttcttt gctttttaac aatgtacaaa gagttttttt attgtttttt
     6721 acatcaaaat cgtaatccat cagtgcgtgt gtgtgcaaaa gcccaactgt taaaagccct
     6781 gtagaaatcg ataaaagcaa aatgtcggaa gaaaatccgc ccaacattac aaaagaagtt
     6841 cccataaaca aaagcggtgc aaaagtcatc ccgactgcaa tttcgcccaa tcctcggtat
     6901 gtgagaaaag gataaaacgc acagaataaa gaagctacaa aagcaattaa caaaacttgc
     6961 cagccgcatt taaaggcaag gaaaattcct ataaaaattg ctattgcaaa gaaaacactc
     7021 agtgcgcgga aggtttgttc cagtgtggct tttccttcta tcatgtattt gcatttgccc
     7081 ttttgcattg ttatgctttg ggggtcattt tgaaattttt gttctttgag ttcctcttta
     7141 taatcgaaat aatcatcaac aaggttaacg cccatatggg cagacattat tccgataagc
     7201 gcgaggattc cgtaaaagaa atctccgccg atgctgtatg accagcaaaa aaccacaagc
     7261 cacgacataa aactcatagg catggtgtga acccttgcgg ctttaagcca aaaaataatc
     7321 ctgttaaaat tttccattta aatttctcct tgataattat cattttaaat gatatcaaag
     7381 aaaacaaaaa taggaaatta aaaattaatt ttcatgttta aatccgattt cttttgtttt
     7441 tttatgttta taaaaacttg gatcctcaac ttttaaatcg gagctttcat caattatgtc
     7501 aaagaaatct tgtttggtta tatctctacg attatcttta agtgctttaa ttgcggcttt
     7561 atctgttata tcgacaatgg ttctgtttga aaatccttcg aaggcttttg caacggcaca
     7621 aatatcatcg gacgaagaaa gcagattttg tgctttttgt ctttcttcaa gtgctttttc
     7681 caagacttct tttctggtat tttcatcggg aagtcctatg tgaaagtgcc cgtcaaatct
     7741 ttgtcttatg gcctcatcca ttaaatcata tttgtttgta gcacctatta ctataatatt
     7801 tcttgcacgg gctgtttcta taaggttaag cagggtgctc atttgtttta aatcttctgc
     7861 atggtcactg ttgtctctgt tttttgtaac cccgtcaatt tcgtcaataa ataaaaagca
     7921 aggttttcct atttttttag catgctcttc tacttgctta aaagcgtttt catagttttc
     7981 agaagttttg ttaatgtaag atgaaccaag ttcacttact ttaaacttga acaatggaat
     8041 atcggcttga atggccaaag cttctgtaat aaaagttttt ccacatccgg gaggtccgta
     8101 aaataaaatt gctttcggaa attttttacc gtattccaat ctatctattt gtgcttgacg
     8161 cggatctttt atagggtcaa taattttttc tttcaaatct gtcaacaaat ctaacattcc
     8221 gcctatactt acaaaacctt taggtgaata ttcattaggc ttaaattcgg agataaggct
     8281 tttttcttct tttttagaat tgggattcat agaccctgtg agttcttctt gaacaatatt
     8341 cataactttt gtgtattttt catcaaattc ttgttttaaa agtttaattt caagattttc
     8401 ttctgtaggt tcataaatat cataaagaca ggaggcgata tttatacgcc aatcctggtt
     8461 gtcttttttg acagcagtat tgtttaaata tccttcagct tcagttgcaa catagttaag
     8521 cttagggaaa agagcttcca tcatttgggc aaaaacatat ttaaaacttg caagcattct
     8581 aaccaagtta tctatttcat agattggggc aaggtttttt tctctttcaa ttatggtatc
     8641 ttcaagttga ttacaaaatt catttgttgc taacaaggtt ttattaaaag gggtataatt
     8701 cttttctaaa gtggctttaa ctttgttatt taattttttg tcgtatccaa aggtcggatt
     8761 gtttaaattg tttattctgt ttactagcaa agtttttctc caaaaaatat cacatggtag
     8821 taaaaaccgt gaaagaaaaa tttgcgggta aaaaaaataa atttaaaaaa tctttacaat
     8881 taagattaaa ccagattaaa atcgccgtgt tttttgatgt gttctatcag cccgccgtct
     8941 tcaagaagtt ttatcataac ttccggaagc ggagtaaatt ttatttgact gttttgagtt
     9001 aagtttttta cgattccgct ttttaaatct aattcaagct catcgcatgc attaatcaaa
     9061 tcagtatcac attcaatgag caaaagccct atgttgattg catttctgta gaaaattctt
     9121 gcaaaagatt ttgcaagaac tgcttgaact ccggctattt taataatctg aggagcatgt
     9181 tctcttgatg agccaagtcc gaaattattt cccgcaacca caaaatcgcc tttttgcatt
     9241 ttatttgcaa attcagggtc agcgtcttca agtacgtgtt ttgcaaattc ttccaaattg
     9301 gagcgcaaat gaaaaagcct tccgggtgct atatgatcgg ttgaaatatt atcaccaaat
     9361 ttatatgctt tgccgttcaa tacgtttcct ttcatgtcat gctgaattta tttcagcatc
     9421 tttcaaacct taaatgccag ttagattctg aaacaagttc aggatgacat cagatgcgct
     9481 tgtttcttgt tcgattaaaa gtataacaaa aactaaatat gttgtaaaat ttcggaaaaa
     9541 atagtaatat ataattatgt ttaacagaaa gtgcaaaata acttttatag cacacggcgc
     9601 gactattcat tctgaagaaa accgcgtttc ggacaaagaa aattatccgc cgataaatga
     9661 agcaggcgaa gaagaaatgg caaaaatctg tgaatggctt aaaaaacgtg ccataaaaac
     9721 agataaaatt tatacaagcc ccgctctcag aaccattcag tctgcaaaaa ttatttcaaa
     9781 agcacttaaa attgattttg aaatcttaga aacacttact tcaaagaaaa atgaagaaat
     9841 ttcggatttc accaaaagaa tttcaacggt aattaagcaa attgttgaag aaaatatcgg
     9901 caaaagaatt attatcgtaa cctatcctga agtaattcaa gcggcaatat cttctgcaat
     9961 aaaacttggt gctaaaaatc agacaaacat ttatataaaa acaggaagcg caagccaaat
    10021 aagctacttt gaaaactggt caagccttgt ttattcgggt tgtgtgcctt tatagttaaa
    10081 taattatttt gtaaatgtca tttttgttta aatcgtaaag ttcggctaaa atagttgaaa
    10141 tgtcttttgc gccgaatttt ttgcgtttta aaagctctat tttttctaaa atctctgttt
    10201 gacaaaattc ggtgctttct tttttgtaaa ccattacaac gatttcgcct tttaggggat
    10261 tggctttata atattcaatt acattttgaa ctttgtctat tacgatttct tcaaaaactt
    10321 ttgtcaattc tctgcctact gcaactttgg ctttaggatt aaattcattt atcccttcaa
    10381 gtgtttttaa aaggcggttg ggggattcgt agaaaacaag atttgtatgg gcaaattttt
    10441 gcaaaatcgc aaaaatttgc cctttagttt ttggtaaaaa ccctataaag gcaaaatctt
    10501 cgccgtctct tgggacttgc gacagaaaag tcggaacagc acacgctcca ggaagcgcag
    10561 taaaattaat cccatttaac cccaattcag agattaaaat actcccgggg tcacttatta
    10621 aaggagttcc tgcatctgaa acaagggcga tttttttacc ttctttcaac agttttaata
    10681 ataaatttac cctttgtttt tcgttgaatt tgtgataaga aattaatttc gttttaatat
    10741 cgtagcgata gagaagtttt tgggtaattc gtgaatcttc gcaggcgatt atatcgactg
    10801 acttaagaat atcgattgcc cttagcgtta catcttcaat attgcctatt ggagtaggta
    10861 ctatgtataa ttgaaattct tttttcataa gtcaaaatta ccttaaaaac ctttaatttg
    10921 caaaccgaga aattttcata taaaattttc caagggggat ttttccatga ggttgggcaa
    10981 aaaatattta ataacgcttt ttattttatt tattttggtt gttatttttt atatttcaaa
    11041 aaaaacggtt gaatttgttt atgtaaatcc taacgttaac acgcaagtct tgccctttat
    11101 ttctcctgtt ataaaacaaa ttgatgatgg ggtgaatttt tctgttttaa attgcaattt
    11161 agacaaacaa gaaatgaaga ttttgtatca aaatgcttac ggttctgggt ttttaaaaca
    11221 taaccccata ccaaatgacc ttgattattc tgtggggatt catttgggca agtactttta
    11281 tgacggcaaa aacgctttag aaatcgcaaa agatatagac aataaaatgt cttgttttca
    11341 gattaatttt tatgattata tttcaaacaa taaaaatcaa ggtttcttaa ccgagcttga
    11401 tacactcggt tcaataattc attttgataa aaaaagttct gaaaatatta aatttataga
    11461 acaatctttg cctgaaattt tcaaaggcag ggaatacatt gtttatttaa aaaaaacctt
    11521 tcccgacaag cccggctttg tgcttgaata tccttttgtg ctaaagccca atgaaatttt
    11581 aattgaagat tatgcgccgg ttcagctttt tgcaaaaggg gtaagatata ataaaaaaac
    11641 gcaagatttt ttaagagaaa ttacaatcgt tgttgatttt tatgcagata tagaagatgt
    11701 aaaaacaaaa aaaacaaaaa cagttgagat tgtcgcagaa tcttttgccg gacagaggct
    11761 tcaattatca agaagatttt ttcctccgat tgtttttgta ggagaaaatt cggcaaggta
    11821 tttaaaaaat cttgattatt tgaaagaaga tgaaaaatac cttgaatata gactttttaa
    11881 ttttaaaagg catttacaag agttttctaa tttgaatgaa tttaatgacc gtcccgttaa
    11941 aatgctcaaa aggattttgc aatgcactga gataatatct cccgctttgg atgaaaaaac
    12001 caaagatgag atttatgaaa acatttatca aaatctaaaa aatcctaatg ttattatttt
    12061 aaacgattat tccaccgcac tggggaattt aatccaaatt acacaaatgc ctaaattata
    12121 tgcaaaagct cttaattccg gcgatttgca aaaacttgtc gagtcgatga gagaagattt
    12181 aaacaaattg aagccgacaa attcacaaga cgttaaagag ttaattgaat ttcaagatta
    12241 tatagaaaaa aatattttaa cggtcaaaag caatgaagac cttgtttttt ttagtcaaaa
    12301 tcttctttta aaaatggata gatataataa aaacattatt aactttttta ataaaaatgt
    12361 tacaaatcta aaacaaataa tttcatatat agatgttttt ggtaaaatat ataaagactc
    12421 cggcttttac aaaattgaac tttattggct tgacagaaac actttaggca ttgtaaaaga
    12481 cgattttaca aaaaatattg cgcaaaatga gttaaaagaa tttgcaaggc aaaacaaact
    12541 tgccgatgta aattataaac tgatttcaaa agttcaagcc ccaaaattga gtgtaagata
    12601 tgaaacttgg gtaaaatatc attctacagc cgaggaagaa aaaaattggc agcttctcaa
    12661 aagtcaactt ttgaatgata aaaagaattt taaaataaga aggaaaatat tttttataaa
    12721 gaaatattat taaagcaaag attcttattt attaactttt attttagatg aaagtaaaga
    12781 aaaaatgtta gttcaaaata tcaatttatt aaatataaga aaccgttacc gaccaaatac
    12841 gccccaaaaa attaatttta aaggcaaaga cggattgcct gacaattttg gcattgtaga
    12901 tgagggaaaa ttgtatagaa gtgcaaatat actttcaaag tcggaatttg aaaaactaaa
    12961 agaaaaaggg gttactttta ttgtcgattt gcgtgatcct gctgaagacg gacattcaat
    13021 gttgcatgaa aagagcaggg cgtacgaatt tggtttagag catatatctt ttcctgataa
    13081 tgagttttgc cgtgatatgg aaaaacttta taaaataaca gaagatattt acaagaaaat
    13141 tcaaaaaggc ggtgtcggtt taattcactg cactatgggc gagcagagga caggctgggt
    13201 agcagcggct tataagtttt tgtataaaaa tgccgatttt aaaaccgtag ttgacgatta
    13261 taatagatat tgtccaaacg ctgattccat aagtatttat atgctggact ttataaaaga
    13321 agtcgttgat gctaaaaaat tgcttaaaag catttagctt tggtctaaaa ttttactttt
    13381 aaactgccac attaaaatat agtaaagtgt gcatgaaata attacttttg ctatgataaa
    13441 aagcggttca tgaattaatt tctcaaatat aaaatttgaa attaaaaaac aaacaggcat
    13501 taaaattgtt gccaaaatac ctgtttttaa tatttttgaa gattcaatgt aatcaagtga
    13561 tttgaaattt atcagtgaat ttaagactat taataaaaac acgcaaaata tcattataaa
    13621 acctgcagct gcaagattaa atttgggaag aagaaaaata tttaataaaa ccgcaacaat
    13681 acctacaaaa atacttaagc ccatttcaat gtaagtttta ttgcgtaggt ggtatttgat
    13741 gttcataagt tttaaaaatt cgtgtgcaaa aatcgaaacc gcaaaaaacg gtaataagat
    13801 atgccctgca aaatattttt ccgaaaggat gaattttgca atttcttttg aaaagaaaca
    13861 aaaaattccc acaaaaggaa taaaaattac aaaataaagt tgaagagtcg aggtcacaaa
    13921 ttcatttata agtcgttttt tctcaaactg tttgattatt gtaggaaaca cggaaaataa
    13981 aagagtcgaa aaaatcggtg caagaatatt tgtcgtaaaa aaccagcaaa ttcctgtaac
    14041 agccgtttca aaaaacatgt tattatattg aaaaataaat ttatttatat tcaaaaccgc
    14101 ccaaatactc acgtttgttc cgataagcgg cagagaatat ttaaggcttt cttttaagat
    14161 taagatatcg ggttttatgt taagtctttc ctcaagcccg atttgtttta ttatataaac
    14221 atctatgaca aaaatactta cagtcattgc aataagcagg gcataaatat ccgaatacag
    14281 gtttgagaaa aacaaaaaca ataccaaaag cgataattga taaaaaatta tgctgaaagt
    14341 gtacataaaa ggtttgttta aaatccgcag catttggtat aaaaactgtc ttatgccaca
    14401 ggggattaca agaaaaagtg ttaacagaag agaaattctg tctatggaaa atttttgtat
    14461 aataaaccct gaaagacaaa aataaagaac aaatattaaa atataagata aaaaagttaa
    14521 aaagacgaca ttagacaaaa atttatctgt catttttttg cgtttgtatt tgtgccaaaa
    14581 tctaagtgcg gattttgcaa tccagtcggt agagcaggtg caaaccaaat ttaaaatccc
    14641 gatacaaagc tgaaaaatgc ccatttcgcg cgctgatatg acatgcgcta aaaaagggat
    14701 tatgattagc gaatttaaaa taataaaaaa tcttgaagga aggtacttaa aaaaagtatc
    14761 caaaataatc aggtggtttt tagaaagagc tttttccatg caaaaataat agcataaaat
    14821 tgtgtgttga ggatgcctaa attgctgatt gttttaatat taatatcttt taagcaataa
    14881 ttctttttgc cgaaataact tgaaggttat ccagattaac tttttgctta gcttgccaca
    14941 agtcgtattt aagttgtaat cttaaccaaa tttcgggaga agttccgagc acttttgcaa
    15001 gttttaatgc catttcagga ctaattccta ctttttcatt aacaatttca gacaaatttt
    15061 tacggctggt tcccaagttc aaagcaaacg cggaaaccgt aaaatttaaa ggtttaatgt
    15121 attcttcaag aagtatttcg cccgggtggg gaggattaaa catttccata attttccttt
    15181 cagttataat ctttgtagtc cacaatgtaa acatcctggt ttttaaactc aaaggttatt
    15241 cgccaatttc cgttaacacg catagcccaa agccctttca tattgccttt aagctgatgc
    15301 aaattgtatc cgtcaaagtt gatttccgcc aaatcggaaa tttcatccaa aatagctaaa
    15361 atccgtctga ttttctttgc atgttcgggg ataattcctt ttatgctgcc ggttttaaag
    15421 aaattttcca aacctttgtg tttgaatgat ttaatcatgt actttattat aacatgttac
    15481 gtatcaggtg tcaagcatta tgtttgacaa acttgattaa aaaacctttt tagactaaaa
    15541 tctaagggta gatgaaatta agggagagca ttatgagaaa accgattata gcgggaaact
    15601 ggaaaatgaa taagcttcag gctgaagcaa aagaattgtt tttgggaatt gcatattttg
    15661 cagaaaggtt tacaaaacag cagctgcctg aaattgtttt ggcacctact tttacgtcgc
    15721 ttgcagcagt tgagagcatg agatgcgaca agtgcagagg gcttgtttcg attgcggcac
    15781 aaaactgcca ttgggaaaaa tcgggggcat acacaggaga gatttccgtt gacatgataa
    15841 aagatgctgg ctgcgaatat gttattatag gacactccga aagaagacag tattttagcg
    15901 aaactgatga aatgattaat aaaaaagcaa aagcgatttt agaaggcggg ctgattccta
    15961 ttatctgctg cggcgaaagc ttagagcaaa gagaggcggg gataacagac gctcacatta
    16021 caaaacaaat taaagcggca ctcgaaggtt tatcagaaga ggaaattgca agatctgtta
    16081 tagcttatga acctatctgg gctatcggaa cgggcaaaac ttgtgattct gttgaagcaa
    16141 acagagttat tgcaatgatt agaaatgtcg taaaagaagt aagtacagaa aaagcgggtg
    16201 atgcaatcag gattttatac ggcggaagcg taactcccgc gtcaatcgaa gaacaaatgt
    16261 cacaatctga tatcgacgga gctttggttg gcggggcgag tcttaaaatc gacagcttca
    16321 gtgaaatcat agctaataca atgaaagcga cacaattctt aacaacatag gagttttaga
    16381 agtgtgtcgt tgcgaggaat gaaatgacga agcaatccat ttcatctttg cactttggaa
    16441 agattgcttc gtattttcaa cattattaac actatgagct ctatggctcg caatgacgta
    16501 taaggagtaa ttttatggca aaacaattca gcgggacacc gcaaataaaa acaaggacag
    16561 cacttttaat ttttataaaa ggctcatctg caccggttgt gctttactta gaaaatcctc
    16621 aggcagctta tgaagaaatc caaaagctta taaaagcacc tactgcagcg ttgattgaaa
    16681 aagaggcggt aggtcctatc aaaaaagtgt cacttcaagt tagccaaatt tcagcagtgg
    16741 cactacagga agaacagtac gtttaaaaaa attaataata taaaacaaat aattatgttt
    16801 atgatatgct ttcttaaagt aaacagtgat gagtgaagag tgaagagaat tacttcacac
    16861 ttcacccttt acctctcact aagtcataaa gttaaaaata catacaagga gcaaacaaga
    16921 aaatggcagt accaaagaaa agaacggggc attcggctca aggacataga aga
//
LOCUS       DAOQAU010000035        16849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_3611_length_16849_cov_7.110337, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000035 DAOQAU010000000
VERSION     DAOQAU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 16849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16849
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3611_length_16849_cov_7.110337"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKI94_06050"
     CDS             <1..284
                     /locus_tag="PKI94_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26338.1"
                     /translation="AVNFVKEKLADISDKLNAMFGELKNSAEKKVSDFLKDFKKRIKS
                     LFDLPDLSRADDEDRKLEEDKRIFEFKNITKFIRKRFKNNKKANVKYEN"
     gene            274..1752
                     /locus_tag="PKI94_06055"
     CDS             274..1752
                     /locus_tag="PKI94_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26339.1"
                     /translation="MKTNPINDVENRRQKRNLTDSQKKNADLTREGVLVNSLIKENGI
                     DEIYDTMVISDNARMPQKVYEKDNSKDKSLLPISIIATSVMATMALLSFVAKRSAKVN
                     LNILPEKKLPTLTRNLAINEETHQAIYQMVQCPNQKTILAGVGVLTISAMAFMGKMFL
                     DGFKDIWVKKREADIQKNLQENLIAVETQSFSGKIQIVRCMLADKAREFSEYLNNKPE
                     IPLPFKKMMSFDKVGFKGSEKKNIKKESNLKFFALGAFTLGAVVGFGFLSLKNLRKGK
                     GYIEEYVGKTIEKISEIVKKSDTSTKEIDKINLKNMFQAVDAKAEFVEKKLAELNWDE
                     AEKTKFIDEVLFNTTKSTATVEKTMGGDGTPKPAFYSHVNDYRAFFYNWLLETSNPLF
                     KNLFFAITGVSAVSYGGKVLGEAVKDVQVKKINAQTELELQQRLVSTELRNFKAKKEA
                     AINPLCEEFYTQVKNAKPKEELKTMAENILSEVKNGPPFVYS"
     gene            1981..2595
                     /locus_tag="PKI94_06060"
     CDS             1981..2595
                     /locus_tag="PKI94_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26340.1"
                     /translation="MVQLIKTNLINRAQTNIEYSSAPTYVNIQMPTAQRNYAIFDQNK
                     VNSFVSQRENALPYLKQVLQTSQDEKQIVEALYIVDRMIDAGVKGIPAMYPVFSRFNN
                     TKSPHIQTFLAGIYRKTQVPDAFGPLVKMLIQNSIKNPSPPTPLPQGARVVPTSLLPI
                     IAQSYSSQLTAHGSFFDPNEEIGGAILSYISTYSKGAPKIDYQA"
     gene            complement(2792..4144)
                     /locus_tag="PKI94_06065"
     CDS             complement(2792..4144)
                     /locus_tag="PKI94_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW26341.1"
                     /translation="MGFDEAKQESIFEKARGYGALFSNQSFCMLWLAQIFSQFADRII
                     FVVFVTIIAINFDPTGKSASLQSWLYIAFTIPAILLTAVAGVFIDVWNKKAVMIITNI
                     LRGIIILLLPLFDKTLLSLYALAFLMSSVTQFFVPAEAAIIPSVANKYQLLTANSLFS
                     TTMMASLIFGFALGDPMINICGINFVHYGISLFFFLSALCLCFVNYKTVKKDKENKFT
                     FLEDLIEGLVYIKDNPIVRSAMLKLASLFSVIVMLSILSISLSQNYLYPQNPTLGAQK
                     FAYIVVYCGIGMVFGAFLVGHYFRKVEKHLLGYIGILTCGIALALFGVLPLIPQTSVF
                     HFETKSVLIFYLEQFDFTYPMLYSYIVGALIGVSTAFVAIPTQTILHTEIDPKVRGKV
                     FGILFTLLSTASALPVLIAAWGAQMLGVARMMFILATPALFFSIFKLITYKNKFGENA
                     "
     gene            complement(4145..5107)
                     /gene="secF"
                     /locus_tag="PKI94_06070"
     CDS             complement(4145..5107)
                     /gene="secF"
                     /locus_tag="PKI94_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNW26342.1"
                     /translation="MSEERKYRFDLVKYRIWWIVLSAFLVLPGVFAMIYSSITYPNHA
                     PLKVGIDYTGGTILQYSVDKKVTPHEITELGSKLEKAGIENPYLQNINVNSADKKSEI
                     KSIISIRTKFIDEKNTAQLNKVSDVVKSEYTASKLIQVSSIGPTLGKELFKNSLFALV
                     LACLGIVIYLTIRFQLDFAIAAVLSLLHDALFVMGCFSILGLLFNVQIDGLFITAILT
                     VIGFSVHDTIVVFDRIRENLRYYAKKMSFGEIVNFSVDQTLVRSINTSLTTLITLLAL
                     YFFGGVTTKDFVLAMILGIAIGTYSSIFFASIVIDFWRERKSKK"
     gene            complement(5182..6492)
                     /gene="secD"
                     /locus_tag="PKI94_06075"
     CDS             complement(5182..6492)
                     /gene="secD"
                     /locus_tag="PKI94_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNW26343.1"
                     /translation="MKNKVIMFWAIVIIAIISTIVIFLKPTKLGLDLVGGSRLVLEAQ
                     TTDTVAKITPEMMDSLKYAIENRINKLGVAETVVQQTGEKRLLIEIPNISDLTKAKEF
                     LGETAELDFRKPGPVVNGEQTWLSTGLSGKDLSRAILSTNSTNGQWVVDLQFNDKGSK
                     KFGDLTSKMVGQQMAIFFNGEMQSAPVIREPILGGNAQISGGDTGFQYEEAKKMVDLL
                     NAGALPVPAKIIEENTVGPTLGADSIEKSKVAGMVGLGLVMLFMLIFYRLPGLIADTA
                     LICYSLILFALFKTIPVTLTLAGIAGFILSIGMAVDANILIFERTKEELRAGRTLFTA
                     INAGFDRAFTSIFDSNMSTIITCLILYMLGTSVVKGFALTLALGVIVSMFSAITITKN
                     FMHLIFGTGELKYPQLFGLRKDEIGKAFEVKETKREKAKFGVLE"
     gene            complement(6519..7415)
                     /gene="dapA"
                     /locus_tag="PKI94_06080"
     CDS             complement(6519..7415)
                     /gene="dapA"
                     /locus_tag="PKI94_06080"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNW26344.1"
                     /translation="MPKRFDAGEVITAMVTPFNKNREIDYDKAQELAKFLVENGSDTI
                     LVAGTTGECPTLTHEEELELLSTVKRAVGHRAKVMMGAGSNCTKTAVEAAKTAEKEGA
                     DLILSVAPYYNKPCQRGIFEHYSQIAQSVKIPIILYNIPSRTGITISAQTVSELANKY
                     PNIVGLKQSCPDMDMISELKMLCPEDFVIYSGDDSLTLPMLALGVEGVISVASHLWGR
                     EIKTMIHNFKTGNYVFAKNMHLKLFPVMRKIFMAPNPAPIKAVLARQGLIEDWVRLPL
                     VELTEEQKTELFEVIDEFSNNN"
     gene            complement(7563..8426)
                     /gene="tsf"
                     /locus_tag="PKI94_06085"
     CDS             complement(7563..8426)
                     /gene="tsf"
                     /locus_tag="PKI94_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNW26345.1"
                     /translation="MEITAQMVKELREQTGAGMMDCKKALVETSGDMEKAMEFLRQKG
                     ISSADKKMGRVAAEGRIASVIEGNIGAMVEVNCETDFVAKNEEFKGLSEAFAQRVIDL
                     NPSSPEDLLKQTCPVFHKPIEDVVKEKIATIGEKITLRRFVRYEGNLATYIHNGKIGV
                     LLETDNKDDELTKDICLHIASAAPEFVSKSEIPQSIIDEETRIEMGKDDLASKPEAIR
                     GKIVEGRVQKLFSAKCLLEQPFVKNPDMTIQQLIEGKLTVKRFTRYMLGEGLEKRSEN
                     FAEEVMNQIKS"
     gene            complement(8527..9393)
                     /gene="rpsB"
                     /locus_tag="PKI94_06090"
     CDS             complement(8527..9393)
                     /gene="rpsB"
                     /locus_tag="PKI94_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNW26346.1"
                     /translation="MPTASMIELLEAGVHFGHQTQRWNPKMKPFIYGSRNGIYVLDLR
                     KTTDLLDKAYEVVREFSARNKNILFVGTKKQAAEVIAQEAKRVGAYYINRRWLGGMLT
                     NFETIRSRVNKLRELEDFVSSGHIEKLPKKEIAQLMRQINKLSKTLGGIKEMRGLPDL
                     IFVVDQKKELIAIQEANKLHIPVICLADTNADPDNIDYIIPGNDDAIRSVKLITSKLA
                     DAVLEGKQLRENRAAELSKIQKIEAKDAGVTEEKTEEIIEEVMETVETSQSEEVAEAI
                     ETAEIVEETVEA"
     gene            complement(9638..10279)
                     /locus_tag="PKI94_06095"
     CDS             complement(9638..10279)
                     /locus_tag="PKI94_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26347.1"
                     /translation="MVNNYENFDNLETSIRKSSVIQERVNLVSTHMYKQFLDYQHASL
                     NTNEVFLRMTDDLQAVVETLKQSFSSRGITTQNIYLEVDVQKTVAVINILWHKISFTT
                     RCNFQPQALFRGNEVSPLFSSRIMAVKGSYYELMKNIKDDEMEILLDNEIASLFVPPD
                     KTQNSIMTIRHLANKEFFLNQMDAPREFVLKVTETVCGGGVYHEMGTRKTFNI"
     gene            complement(10415..10487)
                     /locus_tag="PKI94_06100"
     tRNA            complement(10415..10487)
                     /locus_tag="PKI94_06100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10452..10454),aa:Arg,seq:ccg)
     gene            complement(10492..11199)
                     /locus_tag="PKI94_06105"
     CDS             complement(10492..11199)
                     /locus_tag="PKI94_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26348.1"
                     /translation="MNNTFDKTWEENFNNSLNTIPQNHSYEELLELLKSGGVSQRQLA
                     ALELEEIKSKNDAKILVSNLIGQDGKIREAVSFKINEFVKNKNYIQFFIDENIFKKLL
                     QGIMDINGNVCRNIVSCELFLNKDFRGFLCKNLPKKIDEILILIKNLNKDEKQYKISK
                     RNFQLYWSLEALFEIIEEIDLEKIKKILFETAEFKDYTIREKTAKILTRINSPFFVKL
                     KEKLKNDENYYVKRYLN"
     gene            11330..11500
                     /locus_tag="PKI94_06110"
     CDS             11330..11500
                     /locus_tag="PKI94_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26349.1"
                     /translation="MKEFLNFVQNIFMIKDDETMKVGLTQFRRPVIRPVEKDVSIRNK
                     DVKLSDLMRRAG"
     gene            complement(11506..12525)
                     /gene="trpS"
                     /locus_tag="PKI94_06115"
     CDS             complement(11506..12525)
                     /gene="trpS"
                     /locus_tag="PKI94_06115"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNW26350.1"
                     /translation="MNNDQGKKLKIMSGMRPTGKLHLGHYLGVLTNWVKFQDEFDCYF
                     AIADWHALTTKFDQTQDLKQNVVEAVMDWLACGINPEKSTIYVQSLVPETAELSIYLG
                     MITPQNWVERDPTLKDMAKILKNKEGSNNQISYGLMGYPVLMSADIMTFNASVVPVGN
                     DQLAHLEITRDIARRFNYIYKTDFFNEPAPKLTQTPLLCGLDGNKMGKSFNNDIKISD
                     DEETTAKKIMHAVTDRSRVRKDDLGHPDKCEVAFKYWQIFGDEKTVETVKAECQKGLR
                     GCADCKRQLGCVINEKLAPIREKRRYYEAHPAEVERIIKEGSQKAKLEAQKTLEQVRK
                     LVKMY"
     gene            12649..13200
                     /locus_tag="PKI94_06120"
     CDS             12649..13200
                     /locus_tag="PKI94_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26351.1"
                     /translation="MDLKLSFKNVSFEGRGRVICETASGLKKIIIKDGNEEIPDSFSA
                     DFIVQEGGEIFGKLRAKIINFIGGFNNGIEAEELNVCGATLKGDNFVHDTANFYGKSI
                     LDGNLYVKNELKLDNNSKINYGSHVRVEGDTFILDNSLIEGRLITRKLEVAGQPTFSE
                     ISEITVNSEKVNPKNVLKRFRRK"
     gene            complement(13210..14106)
                     /locus_tag="PKI94_06125"
     CDS             complement(13210..14106)
                     /locus_tag="PKI94_06125"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNW26352.1"
                     /translation="MRKFLFYLFILKKAFLLLIYPVFEQKGEIFKTIRKNIENYLIFQ
                     PSKLRALRVQRDLLKKIQPVRFINSDRIKLYAWFLKPQRNMPVFLHFHGQAESILSHQ
                     DIASFCANEGFGIFMLSYRGHYKCWGKPSEQGIYDDAQAAICELKKLGVDADKIILWG
                     HSLGSAVAIETALHNKVAGVILQSPIKEIKSAAIDVYNFYCKRIHLDFVGFFAKAHIQ
                     LINFVQKFDNINKIKDVQCPILILHSKMDRIAPYKNSVALSEQNKNAELFLSEHGSHW
                     DADWCFDKILNFILKNEFVKQI"
     gene            complement(14164..15531)
                     /locus_tag="PKI94_06130"
     CDS             complement(14164..15531)
                     /locus_tag="PKI94_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HNW26353.1"
                     /translation="MTVFNENFILETINMIKLHNLDIRTVTLSLSLRDCISDDVDEVC
                     ERIYNKILRYAKNLDSFATEIENDFSIPIINKRIAVTPISLIGESCKKFDYVKIAKTL
                     DRAAKDVNVDFIGGFSALVEKGFTKGDQALIESIPEALAQTQRVCASINLASSKAGIN
                     MDAVLKMAEIIKKSAELTFEQDGIGAAKLVCFCNSVGDNPFMAGAYCGYGESEYALNV
                     GISGPGVVRAAVEEMPKDADFGELANKIKQISYKITSTGELVGKELAQRLGVPFGVVD
                     LSLAPTPALGDSVAGIFQAMGLEHAGAHGTTAALAMLNDAVKKGGIMASSNVGGLSGA
                     FIPVSEDAGMIEAAKLGHLTFDKLEAMTSVCSVGLDMIAIPGDTPIETIAGIIADEMA
                     IGMINKKTTAVRIIPVPNKNIGEKAVYGGLLGEAPIMQINKLSSSAFVKRGGRIPAPI
                     HSLNN"
     gene            15678..>16849
                     /locus_tag="PKI94_06135"
     CDS             15678..>16849
                     /locus_tag="PKI94_06135"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNW26354.1"
                     /translation="MKVLFASAEVVPFAKVGGLADVVGSLPKYLDKDTEIAVFMPLYG
                     CIDKEKYNIKLLENSRLQISFGWSENIFQLYIGQLPGTKINVFFIDNSKYFSCFNSVY
                     PYNIDERYNQERFIAFSRAILEYTQLLNFKPDIIHANDWHTGMLPVYLETTYRDNEFF
                     KNTKTVFSIHNLAYQGMFYDDVLDFAQIPKAEVMYSWGVELFGHVNWMRGAINYADKV
                     VAVSPTYAKEIQTPEGGYHIDCTLRENSQKLVGILNGIDTKEFDPKTDKLIPKNFDKN
                     DLTGKVACKKFLCEKFNLKYSKNRPLIGMVSRMTEQKGFELFWGIDEELKKLKADLVI
                     LGTGNMWYEDHFNWLSKTTENVRVHLSFSHELANQIYAGCDMFLMPIFDFIMITHH"
BASE COUNT         5189 a         3093 c         3001 g         5566 t
ORIGIN      
        1 aagccgttaa ttttgttaaa gaaaagctgg cagatatatc agataaatta aatgcaatgt
       61 ttggcgagct aaaaaattct gcagaaaaga aagtttctga ttttctaaaa gactttaaaa
      121 aacgtataaa atctttgttt gatttgccgg atttatcaag ggcagatgat gaagacagaa
      181 aattagaaga agacaaaaga atatttgaat ttaaaaatat tacaaaattt ataagaaaaa
      241 gatttaaaaa taataagaaa gcgaacgtaa aatatgaaaa ctaatcctat taacgatgtt
      301 gaaaacagaa ggcaaaaaag aaatttaacc gattcccaaa agaaaaatgc tgatctgaca
      361 agagaaggtg tgctggttaa ttctttaatt aaggaaaacg gaattgacga aatatatgat
      421 acaatggtaa tttctgacaa tgcgcggatg cctcaaaaag tttatgaaaa agacaattca
      481 aaagacaaaa gccttttgcc tataagcatt attgcaacaa gcgttatggc aactatggca
      541 ctgttgagtt ttgtggcaaa aagaagcgca aaagtcaatt taaacatttt gcccgaaaaa
      601 aagcttccga ctttgaccag aaaccttgca atcaacgaag aaacgcatca ggcgatttat
      661 caaatggtac aatgccccaa ccaaaagaca attttagcgg gtgtgggagt tttaaccata
      721 agtgcaatgg cttttatggg aaaaatgttt cttgacggct ttaaggatat ttgggttaaa
      781 aagcgcgaag ccgatatcca aaaaaatctt caggaaaatt taatcgcagt cgaaacccag
      841 tcattttcgg gaaaaatcca gattgtaagg tgtatgcttg cggataaagc aagagaattt
      901 agcgaatatt tgaataataa acctgaaatt ccgcttcctt ttaaaaaaat gatgagtttt
      961 gataaagtag ggtttaaagg cagcgaaaag aaaaacatta agaaggaaag caatttaaaa
     1021 ttctttgctt taggtgcttt tacattgggt gctgttgtag gattcgggtt tttatctttg
     1081 aaaaatttaa gaaaaggtaa aggttacata gaagaatatg tgggtaaaac gattgaaaaa
     1141 atctcggaaa ttgtaaaaaa atccgacact tcaacaaaag aaatcgataa aattaattta
     1201 aaaaatatgt ttcaggcagt tgatgcaaag gctgaatttg ttgaaaagaa attagcagaa
     1261 ttaaattggg acgaggctga aaaaacaaaa ttcattgacg aagttttgtt taacacaaca
     1321 aaatcaacgg ctaccgttga aaaaacaatg ggcggcgatg gaacgccaaa accagctttt
     1381 tattcgcatg ttaatgatta tcgtgctttt ttctacaact ggcttttgga aacttcgaat
     1441 ccgcttttta aaaatctttt ctttgctata acaggggttt ctgctgtttc atatggcggt
     1501 aaagttttgg gagaggcggt taaagatgtt caggtgaaaa agattaacgc acagactgaa
     1561 cttgaacttc aacaaaggct tgtctcaact gagttaagaa attttaaagc taaaaaagaa
     1621 gcggcaatta atccgctttg tgaggagttt tatactcagg ttaaaaatgc aaaacctaaa
     1681 gaagaactta agacaatggc ggaaaacata ctgtccgaag tgaaaaacgg accgccgttt
     1741 gtgtattcct aagtattaca aaaataatta ttcttcctcg ccccttgggg gagaggaaag
     1801 gagcgaagcg acgaggtgag gggtagaata aaatgagcga cgtggcaatc taaaataaaa
     1861 acaatcatcc cttctcccct tgagggagaa ggtgcccaaa gggcggatga ggggtaaaat
     1921 aataaaaacc cctcacccta gccctctccc ccaaggggcg aggggaaagg acaaaaaact
     1981 atggtacaac taattaaaac aaatttaata aacagggcgc agacgaatat agaatattcg
     2041 tctgcgccta cttatgtaaa tatacaaatg cccaccgcac aaaggaatta tgctattttt
     2101 gaccaaaaca aagttaattc ttttgtttcg cagcgcgaaa atgccttgcc ttacttaaag
     2161 caggttttgc agacttcaca ggatgaaaaa cagattgttg aagcacttta cattgttgat
     2221 aggatgattg atgccggagt taagggaatt cctgcaatgt atcctgtttt ttcgcgattt
     2281 aataatacaa aatcaccgca tattcaaact tttttagccg gaatttaccg taaaacacaa
     2341 gttcctgatg ccttcggtcc gctggtaaaa atgcttatac aaaattcaat taagaacccc
     2401 tcacccccaa cccctctccc gcaaggggcg agggtagtac ctacaagtct cttgccaatt
     2461 atcgctcaat catacagctc acagctcaca gctcacggct cattttttga ccctaatgaa
     2521 gagattggtg gggcaattct gtcctacata agcacttatt caaaaggcgc accaaaaatt
     2581 gattatcagg cttgattatt tattatatcc cctcgcccct tgcgggagag ggaaattttt
     2641 caatgtgagg tatcgagcat tagaaaaatg ggtgaggggt aaaaagcgcg cattaattgc
     2701 aacacccctc acccgaattt ctaacactca ccagttcgtg ctgaaattct gccctctccc
     2761 gcaaggggcg aggggaagtt ttttttattt tttaagcatt ttctccgaat ttatttttgt
     2821 aggttatcag tttaaatatg ctgaaaaata aagcaggtgt ggcaagtatg aacatcattc
     2881 tggcaacgcc gagcatttga gcaccccatg cggcaatcaa aacgggaagt gccgatgctg
     2941 tgcttaaaag tgtaaacaaa atcccaaaaa cctttcctct gactttgggg tcaatttccg
     3001 tgtgcaaaat tgtttgggtt ggaattgcta caaaagctgt tgaaacgccg attaaagcac
     3061 ctactatata actgtataac ataggataag taaaatcaaa ttgctccaga taaaaaatca
     3121 aaacagattt tgtttcaaag tgaaaaacgc ttgtttgcgg aattaaagga agtacgccaa
     3181 aaagcgcaag tgcgattccg caggttaaaa ttcctatgta tcccaaaaga tgtttttcta
     3241 cttttctgaa ataatgcccg accaaaaaag ccccaaagac cattcctatt ccgcaataaa
     3301 caactatata agcaaatttt tgtgcgccta aggtcggatt ttgcgggtaa aggtaatttt
     3361 ggcttaagct aattgataaa atcgaaagca ttacaattac cgaaaataat gaggcaagtt
     3421 taagcatcgc agagcgaact atggggttat cttttatata taccaagcct tcaatcaaat
     3481 cttcaaggaa cgtaaattta ttttctttat cttttttaac ggttttgtag ttgacaaaac
     3541 acaggcaaag tgcagataaa aagaaaaata aacttatacc gtagtgaaca aagtttattc
     3601 cgcagatgtt aatcatcgga tctccaagtg caaatccgaa aattaaagat gccatcattg
     3661 tagttgaaaa cagagaattt gcggtcaaaa gctgatattt gtttgcaaca gatggaatta
     3721 tggcggcttc agccggtaca aaaaattggg ttacagagga cattaaaaat gcaagcgcat
     3781 aaaggcttaa aagtgttttg tcgaaaagcg gtaaaagaag gattattatt cctcttaaaa
     3841 tatttgtaat aatcattaca gcctttttgt tccatacgtc aataaaaact ccggcaacgg
     3901 cagtcaaaag aattgcagga atcgtaaaag ctatgtaaag ccagctttgc agactggcgg
     3961 attttcctgt cgggtcaaaa tttattgcaa ttattgttac aaaaacaaca aagattattc
     4021 tgtcggcaaa ttgagaaaaa atttgtgcaa gccagagcat gcaaaagctt tggttagaaa
     4081 ataatgctcc gtaacctctt gccttttcaa atattgattc ttgttttgct tcgtcaaatc
     4141 ccatttactt tttacttttt ctttcgcgcc aaaagtcaat tacaatactt gcaaagaaaa
     4201 tacttgagta agttcctatc gcaataccca aaatcatcgc cagtacaaaa tcttttgtgg
     4261 tcactccgcc aaagaaataa agagccaaaa gagttataag agttgtcaga gaagtgttta
     4321 tacttcttac caatgtctga tcgacactga aattaactat ttcgccaaaa gacattttct
     4381 ttgcgtaata tctgaggttt tctcttattc tgtcaaaaac aacgattgtg tcgtgaaccg
     4441 aaaaacctat tactgttaag attgccgtta taaaaagccc gtcaatttga acattaaaca
     4501 ataatcctaa aattgaaaaa caacccatta caaaaagtgc atcgtgcaaa agtgaaagaa
     4561 ccgcagctat tgcaaagtca agttgaaagc ggattgtaag ataaattaca attccaaggc
     4621 aagctaaaac caaagcaaaa agggaatttt taaataattc tttacccaag gtcgggccta
     4681 tagagcttac ttgaataagc tttgaagctg tatattctga ttttacaaca tccgaaactt
     4741 tatttaattg tgccgtgttt ttctcgtcga taaattttgt tcttattgaa attattgact
     4801 tgatttcact ttttttatct gctgaattta catttatatt ctgcaggtaa gggttttcaa
     4861 ttcctgcttt ttcaagctta gagccgagct ctgttatttc atgaggagtc acttttttat
     4921 ccaccgaata ttgcaggatt gttccgcccg tatagtcaat gccgacttta agcggcgcat
     4981 ggttaggata ggttatgctt gagtaaatca tcgcaaacac tcccggtaaa accaaaaacg
     5041 ccgataaaac tatccaccat attctatatt ttactaaatc aaatctatac tttctttctt
     5101 ctgacattat attctccttt gagtgactaa gtgactaagt gattaggtga ctaagaatag
     5161 tcagtaacct aaacttgtca actattctaa tactccaaac ttcgcttttt cgcgcttggt
     5221 ttctttaact tcaaatgctt ttcctatctc atctttcctc agcccgaaaa gctgcggata
     5281 tttaagttcg cctgttccga aaatcaggtg cataaagttt ttggtaattg ttattgcgct
     5341 gaacattgaa actattaccc ctaaagcaag agtaagcgca aatcctttta ctacgcttgt
     5401 tccgagcata taaagaatta gacaggtgat aattgttgac atgtttgagt caaaaatact
     5461 tgtaaaggct ctgtcaaaac cggcatttat tgctgtaaat agagttcgcc ctgctcttaa
     5521 ttcttctttt gtccgctcaa aaatcaggat gtttgcgtca actgccatcc ctatacttag
     5581 gataaaaccg gctatgccag caagagtaag cgttacggga attgttttaa acagtgcaaa
     5641 taaaatcaaa ctgtagcaaa ttagtgctgt atctgcgatt aagccgggta atctgtaaaa
     5701 tataagcata aaaagcatta ccaagcccag cccgaccata cctgcaactt ttgatttttc
     5761 aatactgtca gcacccaaag tcggtccgac ggtattttct tcaataattt ttgcgggaac
     5821 aggcaaggca ccagcgttta gtaaatcaac catttttttg gcttcttcat attgaaaccc
     5881 tgtatcgccg cctgaaattt gtgcattacc gcctaaaata ggctctctta tgacaggtgc
     5941 cgattgcatt tctccgttaa agaaaatcgc catttgctgt ccgaccattt tagaggttaa
     6001 atctccgaat tttttgctgc ctttatcgtt aaactgcagg tcaacaaccc actgtccgtt
     6061 tgttgaattt gtgctcaaaa ttgctctgct taaatctttt ccgcttaagc ccgtcgaaag
     6121 ccaggtttgt tctccgttta cgacaggtcc gggttttcta aaatcaagtt cggctgtttc
     6181 tcccaaaaat tcttttgcct ttgtaaggtc tgaaatgttt ggaatttcta ttaaaagtct
     6241 tttttctcct gtctgctgaa cgactgtttc tgcaactccg agtttgttga tacggttttc
     6301 gattgcatat tttaagctgt ccatcatttc gggtgttatt tttgcgacgg tatctgtggt
     6361 ttgggcttca agtacaagtc ttgaacctcc taccaaatca agcccaagct tggtgggttt
     6421 taagaaaata actatagtcg aaataatcgc tattattact atcgcccaga acataattac
     6481 tttgtttttc atttttttat cctttgtaaa ttctctttct aattattgtt gctaaattcg
     6541 tctataactt caaaaagttc cgttttttgc tcttcggtaa gctctaccaa tggaagcctt
     6601 acccaatctt caatcaaccc ttgtcttgct agaacagcct taatcggagc agggttgggt
     6661 gccataaata tttttctcat aacgggaaaa agttttaagt gcatattttt tgcaaaaaca
     6721 taatttcctg ttttgaagtt gtgaatcatt gttttaattt ctcttcccca aaggtgtgat
     6781 gccacagaaa ttacgccctc aacacctaat gcaagcatcg gcagggttaa gctgtcatcg
     6841 cccgagtaaa ttacaaaatc ttcggggcag agcattttta attctgatat catatccata
     6901 tcgggacagc tttgttttaa tcctacaatg ttgggatatt tatttgcaag ttcgcttacc
     6961 gtttgggcag aaattgttat tcctgttctt gatggaatat tgtataaaat aatcggtatt
     7021 ttaacgcttt gggcgatttg actgtagtgt tcaaaaattc ctctttggca gggtttgttg
     7081 taataaggcg caacggataa aattaaatct gcgccttctt tttccgcggt ttttgcagct
     7141 tcaacggcgg tttttgtgca gtttgaacct gcacccatca taacttttgc cctgtgtcct
     7201 accgcacgct ttactgttga aagcaattca agttcttctt catgagttaa agtcggacat
     7261 tcgcctgttg ttccggcaac aagaattgta tcagacccgt tttccacaag aaattttgcc
     7321 aattcttggg ctttatcata atcaatttct ctgtttttgt taaaaggcgt taccattgcg
     7381 gtaatcactt ctcctgcatc aaaacgtttt ggcatgaaat atcctcctac tctctactgt
     7441 ctgattatat tacaaagact gaaatagttc attaattatt aagtaaattt accttatatt
     7501 aataaaaata ggcactgaaa aaattttttc aatgcctatt tgtctattag ttataatctt
     7561 aattatgatt tgatttgatt cattacttct tcggcaaagt tttctgagcg tttttcaagc
     7621 ccttcgccga gcatgtatct tgtgaacctt tttactgtta atttgccttc aattagttgt
     7681 tgaattgtca tatctggatt tttaacgaaa ggttgttcca aaagacattt tgcagagaaa
     7741 agtttttgaa ctctgccttc gacgattttg cctctgattg cctcaggctt gctggctaaa
     7801 tcgtctttac ccatttcgat tcttgtttct tcatcaatta ttgattgagg aatttcagat
     7861 tttgaaacaa attcgggagc ggcacttgca atatgcaaac aaatgtcttt tgtaagttcg
     7921 tcatctttat tgtctgtttc aagtagaact ccgattttgc cgttgtgaat gtatgtggca
     7981 agattgcctt cgtatcttac aaatcttctt aaagtaattt tttctccgat agtggcgatt
     8041 ttttctttta ccacatcttc aatgggtttg tgaaatacag ggcatgtttg ttttaataaa
     8101 tcttcagggc ttgaagggtt taagtcgata actctttggg caaatgcttc tgaaagacct
     8161 ttaaattcct cattttttgc aacgaaatct gtttcgcagt tgacttcaac catagcaccg
     8221 atgttacctt cgattactga tgcaatgcgg ccttctgccg ctactcttcc cattttttta
     8281 tcggctgatg aaatcccttt ttgtcttaaa aattccatag ctttttccat atctccgctt
     8341 gtctctacaa gtgctttttt gcagtccatc attccggcac ctgtctgttc gcgaagttct
     8401 tttaccattt gtgctgttat ttccattgtt taatgttcct ttctttattt ttattaaata
     8461 aaaagtaaat aattaacagt gaatagaaat ccatttacca tttactattt actatttatt
     8521 tttatgttac gcttctactg tttcttcaac tatttcagca gtttcgattg cttcagcgac
     8581 ttcttctgat tgtgaagttt caacagtttc cataacttct tcaattattt cttctgtttt
     8641 ttcttcagtg acacctgcgt cttttgcctc tattttttga attttagaca attcggcagc
     8701 tctgttttct ctaagctgtt taccttctaa aactgcatct gcaagcttgg aagtgattaa
     8761 tttaactgag cggattgcat cgtcatttcc ggggataata tagtcaatgt tatccggatc
     8821 agcatttgta tctgcaagac atattaccgg tatatgaagt ttattggctt cttggattgc
     8881 gataagttct tttttctgat caacgacaaa aatcaaatcg ggcaaccccc tcatttcttt
     8941 gataccgccc aatgttttac ttaatttgtt tatttgacgc ataagctgcg caatttcttt
     9001 tttaggtaat ttctcaatgt gaccgcttga aacaaagtct tcaagctctc tgagcttgtt
     9061 gactctcgaa cggattgttt caaagtttgt gagcattccg ccgagccatc ttctgttaat
     9121 gtagtaagca cctactcttt ttgcttcctg cgcgattact tccgcggctt gtttttttgt
     9181 tcctacaaaa agaatgtttt tgttgcgtgc tgagaattct cttacaactt cgtatgcttt
     9241 atcaagcaaa tctgttgttt ttcttaaatc caatacgtaa ataccgtttc tggagccgta
     9301 aataaaaggt ttcattttgg ggttccagcg ttgtgtttgg tggccgaagt gcacacctgc
     9361 ttctaatagt tcaatcatgg atgctgttgg catctttctt ctccttatgg ttggagcata
     9421 acggtggtga gaataaaatc taaaaaccga tacgccctgc gtaataaccg gttaaaccgt
     9481 tccatttttt gaatttatac ttcaaaaaac tagggcaaag cctatcgagc cggtgtaacc
     9541 tcaattacta tgctaacata aacaaaaaaa aacacaaacg agggtgcgga aaaatacaaa
     9601 aatttacaaa ttttagtgtt ttccgttccc ttaatgctta aatattaaaa gtttttcttg
     9661 tacccatttc gtggtaaact ccgccgccgc aaacggtttc cgtaacctta agaacaaatt
     9721 ctcttggcgc atccatttgg tttaagaaaa attctttatt tgcaagatgt cttattgtca
     9781 ttattgaatt ttgggtttta tccggcggaa caaaaagtga agctatttcg ttatcaagca
     9841 aaatttccat ttcatcgtct ttaatatttt tcatcagctc ataataactg ccttttaccg
     9901 ccataatcct gcttgaaaaa agcggagaaa cttcgtttcc tctaaaaaga gcctgaggct
     9961 gaaaattgca gcgggtggtg aagcttattt tgtgccacaa aatgttaatt accgccactg
    10021 ttttttgcac gtcaacttca agataaatat tttgcgtggt aattccgcgc gaagaaaacg
    10081 actgtttaag cgtttcaaca acagcctgaa ggtcatctgt catccttaag aaaacttcat
    10141 ttgtgttaag gcttgcatgt tgataatcca aaaattgttt gtacatgtgg gtcgaaacaa
    10201 ggttaaccct ctcttggatt acagaggatt ttctgataga tgtttccagg ttgtcgaaat
    10261 tttcgtagtt gttgaccaat tttatactct cgagatgtac attattattt tattaacaaa
    10321 aataaattta tgtaaacaat tatacaattt ttttttacaa acgggaatac ttagttaata
    10381 ttatttatca tttttataat tataaaaaga aatttacgct cgatgggaat tgaacccata
    10441 accttcggct tcggagaccg atattctatc cgttgaacta cgagcgcatg attaatttaa
    10501 atatcttttc acataataat tttcatcgtt ttttaatttc tctttaagtt tcacaaaaaa
    10561 cggagaatta attctagtta atattttagc agttttttcc cttattgtat agtctttaaa
    10621 ctctgctgtt tcaaaaagta tttttttaat tttttctaaa tcaatttctt caataatttc
    10681 aaataaagct tcaagactcc agtacagttg gaagtttctt ttgctgattt tatattgttt
    10741 ttcatctttg tttaaatttt tgattaatat taaaatttcg tcaatttttt tgggcaaatt
    10801 tttacaaaga aaaccgcgaa aatctttgtt cagaaaaagc tcacagctta caatatttct
    10861 gcaaacattt ccgttaatat ccataattcc ctgcaaaagt tttttaaaaa tattttcgtc
    10921 aataaagaat tgaatataat ttttattttt cacaaattca ttaatcttaa aggaaacggc
    10981 ttctctgatt tttccgtctt gacctatcaa gtttgaaacc agaattttcg catcattttt
    11041 agattttatt tcttcaagtt ccaatgcggc taattgtctt tgagaaacac ccccgctttt
    11101 taaaagctct aaaagttctt cgtaagaatg gttttgcggg attgtgttca aggaattatt
    11161 aaaattttct tcccaagtct tatcaaaagt attattcaaa tttttatcct ttttttgttt
    11221 agcacaaata tatcacaaat agggatgatt ttttttgaaa aaatagtttt aatgtatgat
    11281 aacggctaga taaaacaggc tataataaga ataaggagat attataccaa tgaaagaatt
    11341 cttaaatttt gttcaaaata tatttatgat aaaagatgac gaaacgatga aagtcggtct
    11401 tactcagttc agacgccccg ttataagacc tgtagaaaaa gatgtttcaa tcagaaataa
    11461 agacgtaaaa ctctctgacc tgatgcgaag agcagggtga gaggattagt acattttgac
    11521 caatttacgc acttgttcta aggttttttg tgcttcaagt tttgcttttt gagaaccttc
    11581 ttttataatg cgttctactt cagccggatg ggcttcatag tagcggcgtt tttctcttat
    11641 gggagcaagt ttttcgttaa tgacacagcc caactgcctt ttacagtcgg cgcagccgcg
    11701 aagtcctttt tgacattcgg cttttactgt ttcaacagtt ttttcatcgc cgaaaatttg
    11761 ccagtatttg aaagccactt cgcatttatc cggatgtccc aaatcgtctt tgcgaactcg
    11821 actcctgtct gtgactgcgt gcataatttt ctttgcggtc gtttcttcat catctgatat
    11881 tttaatatca ttgttaaaag atttgcccat tttgttgccg tcaagtccgc ataaaagcgg
    11941 agtttgagtt aattttggag cgggttcatt gaaaaaatcc gttttgtata tgtaattgaa
    12001 tcttcttgca atatctcttg taatttcaag gtgggcaagc tgatcgtttc cgacaggtac
    12061 gacagaggcg ttgaaggtca ttatatctgc actcataagc acaggatatc ccataagtcc
    12121 gtagctgatt tggttgtttg agccttcttt gttttttaaa atttttgcca tatcttttag
    12181 tgtaggatct ctttccaccc aattctgcgg agtaatcatc cctaaataaa tgctcaattc
    12241 tgcagtttct ggaacaagcg actgaacata tattgtcgat ttttcgggat taattccgca
    12301 tgcaagccaa tccataaccg cttcgactac gttttgtttt aaatcttgtg tttggtcaaa
    12361 ttttgtcgtc agcgcatgcc agtcggctat tgcaaaataa cagtcgaatt catcctggaa
    12421 tttaacccag ttggtcaaaa ctccaagata atgccccaaa tggagttttc cagtaggcct
    12481 catccccgac atgattttta gttttttgcc ttggtcgtta ttcataaata tattttacca
    12541 aaaagttgaa gaattaatta ggtttgtaaa tttttactaa cagatagcaa taaaaatttt
    12601 ttttggtttt aattaattgt gtgatgcaaa aaaataaggg tctaagtttt ggatttaaaa
    12661 ctaagtttta aaaatgtttc gtttgaaggc cgcggcagag ttatttgtga aactgcttca
    12721 ggcttaaaaa aaataattat caaagatgga aatgaagaaa ttcccgacag ttttagcgca
    12781 gattttattg ttcaggaagg cggagaaatt ttcgggaagt taagagcaaa aataattaat
    12841 tttataggtg gctttaacaa cggaattgaa gcagaagaac ttaatgtttg cggcgcaaca
    12901 ttgaaggggg ataattttgt acacgataca gccaattttt acgggaaaag tattttagac
    12961 ggtaatttgt atgtaaaaaa cgaacttaaa ttagataaca attctaaaat taattacggt
    13021 tcacatgtta gggttgaagg tgatactttt atcttggaca attctttaat agaagggcgt
    13081 ttaattactc gaaaacttga agttgcagga caaccgacct tttctgaaat ttctgaaata
    13141 acagtcaatt cagagaaggt caatccaaaa aatgttttaa aaagatttag aagaaaataa
    13201 gcttttttat caaatttgtt taacaaattc attttttaag ataaagttta aaattttgtc
    13261 gaaacaccag tcggcatccc aatggcttcc gtgttctgac aagaataatt ctgcattttt
    13321 attttgctcc gaaagcgcaa cagaattttt ataaggggca attctgtcca tttttgaatg
    13381 tagaattaaa atcgggcatt gtacatcttt aattttgttt atattgtcga atttttgaac
    13441 aaaattgatg agttgaatgt gcgctttcgc aaaaaagccc acaaaatcaa gatgtattct
    13501 tttacaataa aaattataaa catcaatcgc agcggattta atttctttaa tcggagattg
    13561 cagaattact cctgcaactt tgttatgcag tgccgtttct attgcaacag cactaccaag
    13621 cgaatgtccc cacaaaatta ttttatccgc atctactcca agctttttta attcgcaaat
    13681 tgcagcctgt gcatcatcat aaatcccctg ttcgcttggc tttccccaac atttatagtg
    13741 tccccggtat gagagcataa aaataccaaa gccttcattt gcacaaaatg atgcaatgtc
    13801 ctggtgactt aaaattgatt ctgcctgtcc atgaaagtgt aaaaatacag gcatgtttct
    13861 ttgcggtttt aaaaaccatg catagagttt tattctgtcg gaattaatga aacgcacggg
    13921 ctgaattttt ttaagcaaat ctctttgaac tctaagcgca cgaagttttg acggttgaaa
    13981 aatcaaataa ttttcaatat tttttcttat tgttttaaat atttcgcctt tttgctcaaa
    14041 aacagggtaa atcaaaagca aaaaagcttt ttttaaaata aacaaataaa agagaaactt
    14101 tcgcatttca ggattatacc atttttactt aaagagcgca ataactggtt tttacagaaa
    14161 taatcagttg tttaagctgt gaatgggggc aggaattctt ccgcctcgtt taacaaaagc
    14221 cgaagatgag agtttattaa tttgcattat tggtgcttca cccaagagtc cgccgtaaac
    14281 agctttttcg ccgatatttt tattcggtac tggaattatt cttacagccg ttgttttttt
    14341 gttaatcata cctattgcca tttcatctgc gataattccc gcgattgttt caataggcgt
    14401 gtcacccgga attgcaatca tatcaagccc tacagaacaa acggaagtca tcgcttcaag
    14461 cttatcaaaa gttaaatgtc ctaattttgc tgcttcaatc attccggcat cttcagatac
    14521 cggaataaaa gcccctgaaa gtcctccaac gtttgaactt gccataattc cgcctttttt
    14581 aactgcgtca ttgagcatcg caagtgccgc tgttgttccg tgtgcgcccg cgtgttcaag
    14641 tcccattgcc tgaaaaatcc ccgcaacact gtcacccaag gcaggtgtgg gcgcaagtga
    14701 taaatctact actccaaaag gtactccaag cctttgggca agctcttttc ctacaagttc
    14761 accggtcgag gttattttgt aggaaatttg tttaatctta tttgcaagtt ctccaaagtc
    14821 agcgtctttt ggcatttctt caacggcggc tcttaccact cctggacctg aaatacctac
    14881 attcagtgca tattcgcttt cgccgtaacc gcagtaagct cctgccataa aagggttatc
    14941 tccgaccgaa ttgcagaaac acacaagctt tgcagcaccg attccgtctt gttcaaaagt
    15001 taattctgct gattttttga taatttctgc catttttaaa acggcatcca tatttattcc
    15061 cgcttttgaa cttgcaaggt ttattgaggc gcaaaccctt tgggtttgcg ccaaagcttc
    15121 aggaatactt tctataagtg cttggtcacc ttttgtaaat cctttttcta caagtgccga
    15181 aaatccgccg ataaaatcca cattcacatc ttttgccgct ctatcaagag tttttgctat
    15241 ttttacatag tcaaattttt tgcatgattc gcctattaat gaaatcggag taaccgcaat
    15301 ccgcttattt ataataggta ttgaaaaatc gttttcgatt tctgttgcaa aagagtccaa
    15361 gttttttgca tatcttagga ttttgttata aatcctttca caaacttcat caacatcatc
    15421 ggaaatacaa tctctgaggc ttaaagaaag cgtcactgtc cttatatcca aattgtgaag
    15481 cttaatcata ttaattgttt caagaataaa attttcgtta aaaaccgtca tagccgcccc
    15541 ttttctgttg tgcaaattat aacacgaaaa acaaatgccg gaagacataa tcaaaaattt
    15601 ttcaaaactc gcattgattt tttatataaa atcatatatt atttatagac aggttgtatt
    15661 cagacaagga ttttttgatg aaagtattat ttgcctcagc cgaagttgtg ccttttgcta
    15721 aagtcggagg gcttgcagat gtggtcggaa gcttgcccaa gtatttggat aaagacacag
    15781 aaatagctgt ttttatgcca ctttacggtt gcatcgacaa agaaaaatat aatattaaac
    15841 tgcttgaaaa ttccagactt caaataagtt tcggttggag cgaaaacatt tttcaacttt
    15901 atatagggca attacccggg acaaaaatta atgtgttttt cattgataat tcaaagtatt
    15961 tttcctgttt taacagtgtt tacccttaca atatcgatga aagatacaat caggaaaggt
    16021 ttatcgcttt ttcaagggcg attcttgaat acactcaact tttaaatttt aagcccgaca
    16081 taattcatgc aaacgactgg catactggaa tgctgcctgt ttatttggaa acgacttaca
    16141 gagacaacga attttttaag aacacaaaaa ccgtcttttc aattcataat cttgcctatc
    16201 agggaatgtt ttatgatgat gtgcttgatt ttgcgcaaat tccgaaagct gaagttatgt
    16261 attcgtgggg cgtggaactc ttcgggcacg taaactggat gcgaggtgct atcaattacg
    16321 cggataaagt tgttgcggtt tcgcccactt atgcaaaaga aattcaaaca ccggaaggcg
    16381 gctatcacat agattgcact ttaagagaaa attctcaaaa acttgtaggg attttaaacg
    16441 gaatcgacac aaaagaattt gaccccaaaa cagacaaatt aattcctaaa aactttgata
    16501 aaaatgattt gaccggcaag gttgcttgta agaaattcct ttgtgaaaaa ttcaacctca
    16561 aatacagtaa aaacaggcca ttgataggaa tggtttctag aatgacagag caaaaaggat
    16621 ttgagctttt ttggggtatt gacgaggaat taaaaaaatt aaaagccgat ttggtaattt
    16681 taggtacagg aaatatgtgg tacgaagacc attttaactg gctttcaaag acaacggaaa
    16741 acgttcgcgt acatttaagc ttttcgcacg agcttgcaaa ccagatttat gcgggatgtg
    16801 atatgttttt gatgccaatt ttcgacttca tcatgatcac ccaccataa
//
LOCUS       DAOQAU010000036        16484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_3723_length_16484_cov_6.599306, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000036 DAOQAU010000000
VERSION     DAOQAU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3723_length_16484_cov_6.599306"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKI94_06140"
     CDS             complement(<1..702)
                     /locus_tag="PKI94_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26355.1"
                     /translation="MKNLLKLFLLLLFASFFTLKSWAFEAWNNDLKTLFLSKKAVIYA
                     VNIRTFGAKDLDRNGIIEENLGEESGNFTNAINRLDDLKASGVNTILLMPITPTGKIK
                     ALGTAGSLYAAASFNEINPQLKTPNSKLSIDDEAKNFINECHKRKIRVIVDLPCCGAY
                     DLYLKRPELFKKDKAQNPIVPVDWTDVRLLNAGDEQNINQDVYSLYAQFVDMALALGI
                     DGIRADVPALKPAAFW"
     gene            1056..1670
                     /locus_tag="PKI94_06145"
     CDS             1056..1670
                     /locus_tag="PKI94_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26356.1"
                     /translation="MITRKPAEISFGGFSGLYKSKGFKAFLETATDNQPVFSAIFAVL
                     LTCILRPAAIMGLPGKKNKDDKKYAAAHSMASGVMGYILSLIIFSPLAAAMKKIGKNP
                     QKYIGDTAEYLGDKASKSFQGTKEFKMASSIMKMGTEAILAAPRAIVTVALIPPILKY
                     IFGWEKKSSSKEKISAPQNYAAINFKSKKYNHKKVFQNFMGVNK"
     gene            1667..2581
                     /locus_tag="PKI94_06150"
     CDS             1667..2581
                     /locus_tag="PKI94_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26357.1"
                     /translation="MKITFNNNYISSTKYSSNKNLRSKSKPNFTGGNSFLKVLFDPFS
                     DSNNKAVNWLTDKIAKGYAPILRSKPVEKFVTKYKNTNITAHLSAVIASTITLFYIKK
                     TLENDKLDSKKRTTLAINQALVAIVSLLGSYKLEKIVAKPIDGYDEIITKKVDGKKIK
                     EKGKHIEGFVDKFRKVNTEEINAEAKNIKLEDIDVKKLELEELLRSKKIDDSQIESLI
                     KNPVLETLKKHLSAEELIEAERIKRLGKIDKWVDGIRVAKTLIIFGFIFRFFAPVVVT
                     PIANAIGNKLNAKKEPKTVEQKPAEIKK"
     gene            complement(2593..4062)
                     /locus_tag="PKI94_06155"
     CDS             complement(2593..4062)
                     /locus_tag="PKI94_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNW26358.1"
                     /translation="MAAQLLESAFGKNDIRGIYGKDITEELFYYIGKGFVQYIGAKSR
                     KTPAQMWITVTQDARNHSPALAKALINGVVSTGANVVDLGLAPTPIGYYSEVVGIDPS
                     VKGDEEITGALIITASHNPSQYNGLKMTFNKQSLNEFQIKEVKEYTLQEAQKAVLSTN
                     VGSLKHYDLIPDYVEVMKKNFGRVGEGIKVVVDSANATGGVVAPNLYRALGCQVVELY
                     SSPNGNFPHHHPNPSDERTLTYIKKKVLETGADFGIAFDGDADRIGVIDSKGKSMTGD
                     KLLLIYAQDVIEKLKDAPKKPIIVSEVKCSQVLYDKINELGGEAVMCKTGHGFIKGKM
                     KETGAVLAGEMSGHIFFKDRYHGFDDAVYAGCRIIEIVANKKRENPDFKIEQLLKPFD
                     EVFSSQEVRFPCPDELKRPTLKDFERIVNKNPDLFGEKIKEIVTLDGLRIIFDGGFAI
                     IRQSNTEPVFTLRFEAKTQEKAQHYEDVMLGELKKILKV"
     gene            4235..5125
                     /locus_tag="PKI94_06160"
     CDS             4235..5125
                     /locus_tag="PKI94_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNW26359.1"
                     /translation="MNMKNFLSKVSNNASFAGWVFILPALLGTFIFIIIPVICSLGLS
                     FMDWDLLTKAKFVGLNNYKEIFTEPLFGKILLNTVVYALSTSVFAVIIPLFLACILNS
                     KIRGSEFYKTAYFLPFITPMIVIAVIWQWIFDPNIGILNYVLKLNINWLYDVNFAMPA
                     LIIVSVWKLIGYNMVIFLSGLSAINQSLFEAAKIDGANFFDTFRFITVPLLSPTIFFV
                     VIITCISSFQVFDLIYLMTQGGPLESTNVLVYAVYKNAFEYFKIGKASAIAYVLFFII
                     LILTVAQWQLRKKFVYNEND"
     gene            complement(5122..6594)
                     /gene="nadE"
                     /locus_tag="PKI94_06165"
     CDS             complement(5122..6594)
                     /gene="nadE"
                     /locus_tag="PKI94_06165"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNW26360.1"
                     /translation="MKIALAQVKLRTGDFENNFKKVIENIEAAVKSKAEIVIFPQQTL
                     CSAGIKDLCMDREFLKKQDDFLNKIAEKKYKIPVILGSSAFCFDKCYNKFLVVVDGKI
                     QVLEGGKFTFKDKNLVISENLILNTACDVFVWAKNAPFTIESNRKNIAAAKKFLKGHG
                     CKFVYANAVAMADENIYAGQSFALNEKGEQVWQSPICVEELKIINFSAKYKEPFYCFE
                     QEVFEVLTFALREYCEVFGFKKVLLGLSGGIDSALTAALAVSALGNKNVSGILMPSMF
                     SSEGSVKDSEKLAKNLGIKTRTMPITELFDHFIEDVAKERKHDVAEENLQARLRGIIL
                     MFFSNREHHLVLSTGNKSEVAMGYATLYGDMVGGYNLIGDLTKTRVYALSNWINRKGE
                     VIPQDIISKAPSAELRPGQLDEDSLPKYKVLDEIIEMYIEKELPVSEIYKNHDRALVD
                     DTIKRIYHAQYKRKQTCMGVRLTQRAFCSNVAIPIVQKFY"
     gene            complement(6606..7469)
                     /locus_tag="PKI94_06170"
     CDS             complement(6606..7469)
                     /locus_tag="PKI94_06170"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNW26361.1"
                     /translation="MNDFQEEEKVSPVNIQRRLQKNKMERKVRQTQMWLKRFRAFARL
                     MTIIFLLFLGYFLLKLPQWYLPKNVFNSLDNPALEILNNKIVPSYKILAALRRTDIPP
                     QPVYLIETDDIKKNIMQLEPVENVFIRRFWFPARLQIIIQERVPIITISPAADVPPIA
                     FFARGGKLIGRDYMPLNKSFKTILVLSYGLRGDDYRNWDNSKIRLIEKIAKAVEVYSG
                     EGVEYIDFRLPDDIYVKLKTANIRLGVLDDNVFERIKKLPAILPQIKTLDKKIKYVDL
                     RWKDASYIKLE"
     gene            complement(7469..8419)
                     /locus_tag="PKI94_06175"
     CDS             complement(7469..8419)
                     /locus_tag="PKI94_06175"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNW26362.1"
                     /translation="MQYSVRSKTDKNSKIAVLCGGMSSEREVSLRSGKNCHAALLRLG
                     FKNAVLVDVGEDIAEKLKEGKFEIAYNALHGKYGEDGCIQGLLEILKIPYTGCGVMAS
                     AVCMNKEYTKKILSTAKIPLIKSVFIQKGEDVLKAVKSLKYPLMVKPVSEGSSFGMSK
                     VENETELVKAVKEAQKYNPDVLIEEYLVGICSTVGVLDDGDKTFATEILELRPKNEWY
                     DYEAKYTKGMTDFILPAEISADMTKKIKELAVKAHKVTGCSGVSRVDFHIVGDIPYVL
                     EVNTSPGMTDTSDLPAQAACMGIEYDNLVLLILNSAGLNK"
     gene            complement(8374..9303)
                     /gene="murB"
                     /locus_tag="PKI94_06180"
     CDS             complement(8374..9303)
                     /gene="murB"
                     /locus_tag="PKI94_06180"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNW26363.1"
                     /translation="MTDIEVKNNFQLKNLTSFKIGGAAEKLYFPKFQQEFVHLLKNLV
                     NPLIFGNWSNVLISSDGIKGEVISTSKLDKFEIKGTKIYADCGVKGPLLAQKAQESGL
                     TGFEFMIGFPGSLGGNIYMNASAHAQCISDFLVSCCVFDTQKKEILVLEKKDLCCAYR
                     SSVLQKKPYILLSAEFELKKAKKEEIDALVERNMEFRKNFQPALNSPNAGSIFKNPEN
                     DSAGRLLDKAGAKELEFGGAKVWQNHANFIVNTGNATSEDVLELMLKMYNLVKDKYTI
                     ELSPEIKYFGEKSKREAQICNILYGQKQIKTQK"
     gene            complement(9300..10697)
                     /gene="murC"
                     /locus_tag="PKI94_06185"
     CDS             complement(9300..10697)
                     /gene="murC"
                     /locus_tag="PKI94_06185"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNW26364.1"
                     /translation="MKNKYYFIAIGGIGMSGLAKYLVEDGFEVLGSDIKENRNIIELR
                     KLGAKIFIGHDENNLPQDCVVIASTAIKENNPELIKARKLGLEVLHRSDMLNRISKGL
                     DKPKKKYFLGYSGTHGKTTTSGISAYVLEKAGLSPSFVVGGILPEYGINSQAGNGDFF
                     VAELDESDGTIVKYQPEISVINNLEVDHVDFYTDGLEGILNTFKKYLDTLPEGAKIII
                     NNDCGGNLKLMEQNPQKQFITFGLKEADYVAKNIEYKGKETSFDIFYKGQFLINLKIQ
                     LRGRHNVYNALAVLASFREANLDIEVLKEHFYTFTGMGRRFQLKAEFDGISVYDDYAH
                     HPTEIKATLDSAQSLHDKKIIAVFQPHRYTRFQGLWDEFLGAFNGVDEVVITDIYSAS
                     EEPIEGVNPQKFAQELFQKTGIKCTHLQGNINEVAQQLLGMLKSNDVVIGLGAGSITS
                     LGAELLKARESISKV"
     gene            complement(11874..12728)
                     /gene="rpoD"
                     /locus_tag="PKI94_06190"
                     /pseudo
     CDS             complement(11874..12728)
                     /gene="rpoD"
                     /locus_tag="PKI94_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(12992..14860)
                     /gene="dnaG"
                     /locus_tag="PKI94_06195"
     CDS             complement(12992..14860)
                     /gene="dnaG"
                     /locus_tag="PKI94_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNW26365.1"
                     /translation="MSSVTFEDVVSQIKDRLDILDVVSKDVILKKSGGNYWGLCPFHK
                     EKTPSFSVNPAKGIYKCFGCGEGGDALSYIIKTRGINFSELIKELASEFGLELPSTFS
                     GVKNKDVKDQMIKACAKAAEFYQQVLFASSEGSTALSYLNNRGIDEKIIKRYSLGLAS
                     KEPNHLYVSLKDKFSDDVLEKAGLVLKSSKGTYTDRFRNRIIIPIHDEMGDIVAFGAR
                     ALEEGQNPKYLNSSDSLIYNKSKILYGLYFAKEAIKQSDSVIVMEGYFDVISAQANGI
                     ENCVASCGTSLTTEHIKLISRYSKSRKIYLSFDTDSAGLKAAQRGAEIIKEAFTGLGN
                     VKQFDESYSSTSNEKYACEIRVITPPEGKDPDEFIRTAGAKEYAKYLEHAPLLLDFQL
                     NQVLKQKNEAKTPTEKLKLVKEIIPILAEIQNKIILSEYIKMVASALNIDEEALQKEI
                     KKIDFQKPLSNNVLNPIVTKSSNIMEKAQKNLLSLYLINESHFTFQQINEMISNIKFD
                     HEKLIIVKTTIDKLICTVNNVKALIEQLYINFVQDEEIKAIITDLICISETFNNLSET
                     DFKAVIDENINKIRQCQREIERNEMRNLYKNVNDDDTEALKVQIQLRDKIKDTFRR"
     gene            complement(15091..16338)
                     /locus_tag="PKI94_06200"
     CDS             complement(15091..16338)
                     /locus_tag="PKI94_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW26366.1"
                     /translation="MKKFFNDYIQTLETYIMFKISEEVARIKPDLISKGRSPIALSLG
                     APVDNPPQFVIDKLKDALDVEGIHSYSSPKGEAFYLEAVAKRMKERFGVDLESKTEVF
                     SLIGSKEGLANFIRALITAKKDEFERDIILIPDPGYASYKEMIKVSGGVGYSIPLTYE
                     NNYMPDMEEVLAQLKKEGYDEKKIKALVINYPNNPLGATATLDYFKKLVEFCKKHNIL
                     LISDAAYTDMYFDENDRPHSVLELEGAKDIAVEFFSFSKPYAMTGWRLGWVCGNSEAV
                     SIFGKLKTTIDTGIFKALQKAASEVLNSKEGEEYIKKANAGFKKKQDIFIKGLSELGW
                     DIDNINIPNATFYLWLPIPPRYKTSKEFTDDLMRTSGIIAVPGDGFGKYGEGFFRISI
                     VCSDENIYEVIDRMKKDGFYFNN"
BASE COUNT         5049 a         3300 c         2687 g         5448 t
ORIGIN      
        1 ccaaaaagca gcaggcttaa gcgcgggcac atccgccctt attccgtcta taccgagagc
       61 taatgccata tcaacaaatt gagcatataa actataaacg tcttgattta tgttttgttc
      121 atcccctgca tttaaaagcc ttacatcagt ccaatcgaca ggaactatag gattttgtgc
      181 tttgtctttt ttaaataatt cgggtctttt taaatataaa tcataagccc cgcaacaagg
      241 cagatcaact attacacgga tttttctttt gtggcattcg tttatgaaat ttttcgcctc
      301 atcgtctatc gaaagttttg aattaggggt tttaagctga ggattaattt cgttaaaact
      361 tgcagctgca tacaaagagc ccgccgttcc aagtgcttta atttttcctg tcggagttat
      421 cggcataagc aaaattgtat ttacacccga tgctttcaaa tcgtcaagcc tgtttatagc
      481 attggtaaaa tttccgcttt cttcgccgag attttcttcg ataatgccgt ttctatccaa
      541 atcttttgcg ccgaaagtcc ttatgttgac agcataaatg actgcttttt tagacaaaaa
      601 aagtgttttt aaatcattat tccaagcctc aaaagcccat gactttaaag taaaaaaaga
      661 ggcaaaaagc aacaataaaa ataattttaa taaatttttc attttatgat tttatcaaaa
      721 aaataaaaaa attttgcaaa tgttaaccat gcattttgga aaaaatttac aattttattt
      781 ttttccgccc ctctctattc ctctatttac cgtattttta aaaaaatatg tgttgaattg
      841 taaatattaa gtgttaaaat aacaaatata aatatgtttc attttaaaat aaatgtatag
      901 acaaataaat tgaatatcaa ttgcaggaga atatgattaa cagtctttcg aaagattcac
      961 atataaccca ctcgttgccg tcggcaacaa accgtaaagg gagtggagtg tgccttaaga
     1021 aaagagagat tggtgtacct gcgaaaaata gtaatatgat tacgagaaaa cccgccgaga
     1081 taagttttgg cgggttttca ggtctttata aaagtaaagg atttaaagca tttctagaaa
     1141 ctgcaactga caatcaacct gtttttagcg caatatttgc agttttatta acatgtattt
     1201 taagacctgc tgcaataatg ggacttcccg gtaaaaaaaa taaagacgat aaaaaatatg
     1261 cagccgctca ttcaatggca tctggtgtaa tgggatatat tttatcctta attatatttt
     1321 cacctcttgc ggctgcaatg aaaaaaatag gcaaaaaccc tcaaaaatat ataggtgata
     1381 ctgcagaata tttaggagat aaagcttcta aatcattcca aggcacaaaa gaatttaaaa
     1441 tggcatcaag tataatgaaa atgggtaccg aggctatctt ggcggctcca agagcaattg
     1501 ttacagttgc tttaatccct ccgattttaa aatatatttt cggctgggag aaaaaatcat
     1561 cgtcaaaaga aaaaatttcc gctcctcaga attatgctgc aataaatttt aaaagcaaaa
     1621 agtacaatca taaaaaggtt tttcaaaatt ttatgggagt aaacaagtga aaataacttt
     1681 taacaataat tatataagtt caacaaaata ttcttcaaat aaaaatttga ggtcaaaatc
     1741 aaaacctaat tttacaggag gcaattcttt tttaaaagtt ttatttgacc cgttttctga
     1801 ttctaacaat aaagcagtaa actggcttac tgataaaatc gccaaaggat atgcgcctat
     1861 attaagaagc aagcctgtag aaaaatttgt aacaaagtac aaaaatacta acattacagc
     1921 acatctttcc gctgtaattg cctcaacaat tactcttttt tacattaaaa aaactttaga
     1981 aaatgataaa cttgattcta aaaaaagaac tactttagca ataaatcaag cattggtagc
     2041 aatagtttca ttattaggaa gctataaact cgaaaaaatt gtggcaaaac ctatagacgg
     2101 ttatgatgaa ataattacta aaaaagttga tggcaaaaaa attaaagaaa aagggaaaca
     2161 tatagaaggc tttgtggata aattcagaaa agtaaatacc gaagaaatta atgctgaagc
     2221 taaaaatatt aaattagaag atattgatgt taaaaaattg gagcttgaag agcttttaag
     2281 aagcaaaaaa atcgatgatt ctcaaattga atctttaata aaaaaccctg ttttagaaac
     2341 tctcaaaaaa catttaagtg cggaagagtt aattgaagca gaaagaataa agcggcttgg
     2401 taaaatagac aaatgggttg acggtataag agttgcaaaa acattgataa tatttggctt
     2461 catcttcagg tttttcgctc ctgtagttgt aacaccaatt gcaaatgcaa tcggcaacaa
     2521 gctcaatgca aaaaaagaac ccaagactgt tgaacaaaaa cccgctgaaa taaaaaaata
     2581 attttttaat ttttaaactt ttaaaatttt ctttaattcg cctaacatta catcttcgta
     2641 atgctgcgct ttttcctgtg tttttgcttc aaacctcaaa gtaaatacag gctctgtgtt
     2701 gctttgtctt attatcgcaa atccgccgtc aaaaattatc ctgagcccgt caagggttac
     2761 aatttcttta attttttcac cgaacaaatc ggggttttta ttaacaattc tttcaaaatc
     2821 tttcaaagta ggtcttttta attcatcagg acatggaaat cttacttcct gagatgaaaa
     2881 aacctcatca aagggcttca acaactgttc gattttaaaa tcggggtttt cacgtttttt
     2941 gtttgcaacg atttcaataa ttctgcatcc cgcataaacc gcatcatcaa agccgtgata
     3001 cctgtcttta aagaaaatgt gtccactcat ttcgccagcc agcactgctc ctgtttcttt
     3061 cattttacct ttaataaaac catgccctgt tttgcacata acagcttcgc cgccaagttc
     3121 gtttatcttg tcatagagga cttgcgaaca tttgacttca gacactataa tcggtttttt
     3181 aggcgcatct ttcaattttt caatcacatc ttgcgcgtaa atcaaaagaa gtttgtcgcc
     3241 tgtcatgctt ttgcctttag aatcaataac tccgattctg tctgcatcgc cgtcaaaagc
     3301 tattccaaag tcggcacccg tttccaaaac tttcttttta atataggtta aggttctttc
     3361 atcgcttgga tttggatgat gatgagggaa atttccatta gggcttgaat aaagttcaac
     3421 cacttgacag cccaaggctc tgtataaatt gggggcaaca actccgcctg ttgcatttgc
     3481 actatcaaca acgactttta taccttctcc gactctgccg aaatttttct tcataacttc
     3541 aacataatcg ggaattaaat cgtagtgttt taagcttccc acattagtcg ataaaacagc
     3601 tttttgcgct tcctgcaagg tatattcttt gacttctttt atctgaaatt cattcaatga
     3661 ctgcttgtta aaagtcatct tcaagccgtt atactgactg gggttgtggc ttgctgttat
     3721 aattaacgcc cctgttattt cttcatcgcc tttaacagaa gggtctattc cgaccacttc
     3781 cgaataatac ccgataggag ttggcgcaag tcccaaatcc acaacgtttg cacctgtgga
     3841 aacaacaccg ttaataagtg cttttgcaag ggcgggagag tgatttctgg catcttgcgt
     3901 tactgtaatc cacatctggg caggcgtttt tcttgatttt gcacctatat attgaacaaa
     3961 accttttccg atatagtaaa aaagttcttc tgtaatatct tttccgtaaa ttcctcttat
     4021 atcatttttc ccgaaagcac tttccaataa ttgcgcagcc ataatatctc catgatttta
     4081 tttccaagca atactatcat ctgcacccac aatgtcatgc tgaacttgtt tcagcatctt
     4141 gccgactttg caatatggat agatcccgaa acgagttcgg gatgacagtt agcttgagtt
     4201 gtattatcta taagctttat aatataataa taacatgaat atgaagaatt ttttatcaaa
     4261 agtgtcgaat aatgcatctt ttgcaggctg ggtttttatt cttccggcac ttttgggaac
     4321 atttattttt ataataattc ctgttatctg ttctttaggc ttaagtttta tggattggga
     4381 tttgcttaca aaagctaaat ttgtaggatt aaataactac aaagaaattt ttaccgaacc
     4441 gctatttggc aaaattcttt taaatactgt agtctatgcg ctttcaactt ctgtttttgc
     4501 ggtgataatt cctttgtttt tagcctgtat tttgaattca aaaataagag gttctgagtt
     4561 ttataaaaca gcttattttc tgccttttat tacgcctatg atagtaatag cggtgatttg
     4621 gcagtggatt tttgatccaa atatcggaat tttaaactac gttttgaaat taaatattaa
     4681 ctggctttac gatgtaaact ttgccatgcc tgcattgata atagtttcag tgtggaaatt
     4741 aataggatac aacatggtga tttttttatc gggactgagt gcgataaacc agtcactttt
     4801 tgaagctgca aaaatcgacg gagcaaactt ttttgatact ttcaggttta taaccgttcc
     4861 gcttttatcg cccacgatat tttttgttgt aataatcacc tgcataagct cttttcaggt
     4921 ttttgacctg atttatctga tgacacaggg cggaccgtta gaaagcacaa atgttttggt
     4981 ttatgcggtt tacaaaaatg cttttgaata ctttaaaata ggcaaagcaa gtgccattgc
     5041 ctacgttttg ttttttatta ttcttatttt aaccgtagcc caatggcagc ttcgcaaaaa
     5101 gtttgtttac aacgaaaatg attaataaaa cttctgcacg ataggaattg caacgtttga
     5161 gcaaaacgcc ctttgcgtaa gccttacgcc catacaagtc tgtttgcgtt tatactgcgc
     5221 atgataaatc cgcttaatcg tatcatcaac caatgctcta tcgtgatttt tgtaaatttc
     5281 actcaccgga agctcttttt ctatatacat ttcaatgatt tcgtccaaca ctttgtattt
     5341 gggaagagaa tcctcatcga gttgacccgg tctgagttct gcagaaggtg ctttagatat
     5401 aatatcttga ggaataacct caccttttct gttaatccag ttagaaagtg cgtaaactct
     5461 ggttttggtt aaatctccga taagattata tcctccaacc atatcaccgt ataaagtggc
     5521 ataacccatt gcaacttctg atttattgcc cgtggaaagt accaaatgat gctccctgtt
     5581 tgagaaaaac atcaagataa tcccgcgaag ccttgcctgc aaattttctt ccgcgacatc
     5641 gtgttttctt tcttttgcaa catcttcaat aaaatggtca aaaagctctg ttatgggcat
     5701 tgttctggtc ttaattccca aatttttagc caatttttcg gaatccttga cactgccttc
     5761 tgaagaaaac atgctcggca tcaaaattcc cgaaacattt ttatttccta aggcactgac
     5821 cgccaaagcg gcggtcagtg cagaatctat ccctcctgaa agtcccaaaa gaactttttt
     5881 aaacccgaaa acttcacagt attctcttaa agcaaaagtc aaaacttcaa aaacttcctg
     5941 ctcaaagcag taaaaaggct ctttatattt tgccgaaaaa ttaattattt tcaattcttc
     6001 cacgcaaatc ggtgactgcc aaacctgttc gcctttttcg tttagggcaa agctttgccc
     6061 cgcataaata ttttcatctg ccatggcaac ggcgtttgca taaacaaatt tgcacccgtg
     6121 acctttaagg aatttttttg cagcggcgat attttttctg tttgactcta ttgtgaaagg
     6181 agcatttttc gcccaaacaa aaacatcaca ggcagtattc aggattaaat tttccgaaat
     6241 aacaagattt ttgtctttaa aagtaaattt tccgccttca agaacctgaa tttttccgtc
     6301 aacaacaact aaaaatttat tgtagcattt gtcaaaacaa aaagcagaag agccaagaat
     6361 tacagggatt ttatattttt tttcggcaat tttgttaaga aaatcatctt gttttttcaa
     6421 aaattccctg tccatgcaca aatctttaat tcctgccgaa caaagcgttt gttgaggaaa
     6481 aattacaatt tccgccttac ttttgacggc agcttctata ttttcaataa cttttttaaa
     6541 attattttca aaatctcccg tgcgtaattt aacttgtgcc aaagcaattt tcatattttt
     6601 tctccctatt ccaatttaat ataacttgcg tctttccagc gcagatcaac gtatttaatc
     6661 tttttatcca aagtttttat ctgcggcaaa attgcaggaa gcttttttat acgctcaaaa
     6721 acgttatcat ccaaaactcc cagtcgaatg tttgcagtct taagcttaac ataaatatca
     6781 tcggggagcc tgaagtcgat gtattccacc ccttcgcccg aataaacttc aaccgctttt
     6841 gcaatttttt caataagccg aatttttgaa ttgtcccaat tcctgtaatc atcgcctcta
     6901 agcccataag acaaaactaa aattgtctta aatgatttat tcagcggcat ataatctctg
     6961 ccgataagtt tccctcctct ggcaaaaaag gcaatcggag gcacatcggc agcaggcgaa
     7021 atcgtaatta ttggcaccct ttcctgaatt ataatctgaa gccttgcggg aaaccaaaac
     7081 cgcctgataa aaacattctc gacaggttca agctgcataa tattcttttt aatatcatct
     7141 gtttcaatca aataaacagg ctgcggtgga atatcagtac gacgcaaagc tgcaagtatc
     7201 ttgtatgaag gaacaatttt attgttcaaa atctccaaag cagggttgtc aagcgaatta
     7261 aaaacatttt taggcagata ccactgaggc aattttaaca aaaagtatcc gagaaataaa
     7321 agaaaaatta tcgtcatcag ccttgcaaaa gcacggaaac gcttgagcca catctgagtt
     7381 tgacggacct ttctttccat tttgttcttt tgtaagcgtc tttggatatt gaccggactt
     7441 actttttctt cttcttgaaa atcattcatt acttatttaa acccgcactg tttaagatta
     7501 acaacaccaa attatcatat tcaattccca tgcaagccgc ttgcgcagga agatcgcttg
     7561 tgtcggtcat tccagggctt gtatttactt caagcacata aggaatatca ccgactatgt
     7621 gaaaatctac ccttgaaacc cctgagcagc ctgtgacttt atgcgcctta accgcaagct
     7681 ctttaatttt ttttgtcata tcggcagaaa tttcggcagg caaaataaaa tccgtcatac
     7741 cttttgtata ttttgcttca taatcgtacc actcgttttt gggacgaagt tcaaggattt
     7801 ctgttgcaaa agttttatca ccgtcatcta aaacaccgac tgtcgagcaa attccgacca
     7861 aatattcttc tatgagcaca tccggattgt atttttgtgc ctctttaacc gcttttacaa
     7921 gttcagtttc attttcaacc ttactcatcc caaaacttga accttccgaa accggcttta
     7981 ccataagcgg atattttagt gattttacag cttttaaaac atcttcgcct ttttgtataa
     8041 aaacagattt aataagcgga attttagcgg tagaaagaat ttttttcgta tattccttgt
     8101 tcatgcaaac agcacttgcc ataaccccac aacccgtgta ggggattttc aaaatctcta
     8161 aaagcccctg aatacaacca tcttcgccat attttccgtg aagcgcgtta taggcaatct
     8221 caaacttgcc ttcctttaat ttttcggcta tatcttcgcc aacatcaaca agaacagcgt
     8281 ttttaaaccc caaccttaaa agtgcggcat ggcagttttt gcccgaacgc aaagaaactt
     8341 ctctctcaga tgacatcccc ccgcataaaa ccgctatttt tgagttttta tctgtttttg
     8401 accgtacaga atattgcata tctgtgcctc tctttttgat ttttcaccga aatactttat
     8461 ctcgggcgac aattcaatag tatatttatc cttaaccaga ttatacatct tcaacatcaa
     8521 ttctaaaaca tcttctgatg ttgcatttcc tgtgtttaca ataaaattcg catgattttg
     8581 ccaaacttta gctccgccga attcaagttc cttagcacct gccttatcca aaagccttcc
     8641 tgctgaatcg ttttcaggat ttttaaaaat acttccggca ttgggtgaat taagcgcagg
     8701 ctgaaaattt ttcctgaatt ccatatttcg ctcaaccaaa gcatcaattt cttctttttt
     8761 tgcctttttc agttcaaatt ccgcagaaag caaaatgtaa ggctttttct gcaaaacaga
     8821 gcttctgtaa gcgcagcata aatctttttt ctcaagtacc aaaatttctt ttttttgtgt
     8881 gtcaaaaaca cagcagctta ccaaaaaatc gcttatacat tgggcatgcg cagatgcgtt
     8941 catataaata ttgccgccca aagaaccggg gaaacctatc ataaactcaa atcctgtaag
     9001 cccgctttct tgggcttttt gagcaagcaa aggtcctttt acaccgcaat cggcatatat
     9061 ttttgtgcct ttaatctcga atttatcaag cttggaggtg cttataacct cacctttaat
     9121 tccatcggat gaaatcaaaa cattggacca attcccgaaa atcaaaggat tgacaagatt
     9181 tttaagcaga tgaacaaatt cctgctgaaa tttaggaaag tacagttttt cagctgcccc
     9241 gccaatctta aacgaggtaa gatttttcaa ctgaaaattg ttcttaactt caatatccgt
     9301 catactttag aaatactttc tcttgctttg agaagttctg cccccaagct tgtaatgctt
     9361 cctgctccaa gtcctatcac cacatcattt gattttaaca ttcctaacag ttgttgtgca
     9421 acttcgttaa tatttccttg caaatgagta catttaattc ctgttttttg aaaaagttcc
     9481 tgagcgaatt tttgcggatt aaccccttca ataggttctt ccgaggctga ataaatgtcg
     9541 gtgattacaa cttcatccac accgttaaaa gcacccaaaa attcatccca aagcccttga
     9601 aatcttgtgt atctgtgcgg ctgaaaaaca gctataattt ttttatcatg caaagattgt
     9661 gcggaatcaa gcgtagcttt gatttcagtc ggatgatgag cgtaatcgtc ataaacgctt
     9721 atcccgtcaa attcggcttt aagctgaaaa cgccttccca tgcccgtaaa agtataaaaa
     9781 tgctctttca acacttcgat atccaaatta gcttcacgaa aactagccaa aaccgccaaa
     9841 gcattgtaaa cattgtgcct gccgcgaagc tgaattttta aattgattaa aaattgccct
     9901 ttgtaaaaaa tatcaaaaga cgtttctttg cctttatatt caatattttt agcaacataa
     9961 tcagcttctt ttaaaccgaa agtgataaat tgcttttgcg gattttgctc cattaacttc
    10021 aaattaccgc cacaatcgtt gtttataatg attttagcac cttcaggcaa tgtgtccaga
    10081 tactttttaa aagtatttaa aataccctct aaaccgtctg tgtaaaaatc gacatgatca
    10141 acttccaaat tgtttataac gcttatttca ggctgatatt taacgatagt cccgtcgctt
    10201 tcgtcaagtt cggcgacaaa aaaatcgccg tttccagcct gagaatttat accgtattcg
    10261 ggcaaaatcc ctccgacgac aaaagacggc gaaagccccg ctttttcaag cacataggca
    10321 gaaatgccgc ttgtagtggt ttttccgtga gtacctgaat atcccagaaa atatttcttt
    10381 ttgggtttat caagtccttt tgaaatcctg ttgagcatgt ccgagcggtg caagacttca
    10441 agcccgagtt ttcttgcttt tattaattcg ggattatttt cttttatagc tgtgcttgca
    10501 ataacaacac aatcttgcgg taaattgttt tcatcatgac ctataaaaat ttttgcccct
    10561 aattttctta actcaataat atttctgttt tctttaatgt cagagcctaa aacctcaaac
    10621 ccgtcttcca caaggtattt tgccaacccg ctcatcccga ttccgccaat tgcaataaaa
    10681 taatacttat ttttcaactt aaattcctct tcgattatct gtattactaa ttttagtaca
    10741 aaaaaacgta tatgttaacg ctcaggcaaa aaaataaaaa acttaacaat aaaatttaaa
    10801 atattaattt ttgtaaaaac ggcaaatacg tactgtgtag tagtttttaa agaaagtttt
    10861 tatattttaa ttaaaaaaaa attaaaatat aaataaaggg ttgacaaaaa aaaataatat
    10921 gtcataatag aaatatagat ttaagtgtag ctgaaacact aaatagcaaa gtttttaaat
    10981 aatacattta accctaaaca tataaactcc taaatataaa cactaaaaag agaccattag
    11041 gatgcagaaa acgacccact ctatcactag agtggttttt tttgccttct tttcaaggcg
    11101 aggaacgagc cgtagaaaat ggcgtcacga cttgcttata tagttccact ctttagcgta
    11161 gatacacaat agtgacacaa gcgagcagag gcacgaacac tctgccgaag aaaaagatta
    11221 agtatctttt tctgagaggt agagagcgaa caacaataat cggaaacgac gcttttcgat
    11281 tttgttgtaa gcggtgccgt ttattgcgag cgcagtctaa gcaaggcaag cagggcaaca
    11341 ttacgatacc gccgttgtga atgaagtgat aacggaatga acgaagcaat ctttgattgc
    11401 acaggcggtt ttgccttctt ttcaaggcga ggaacgagcc gtagaaaatg gcgtcacgga
    11461 ttgcttagat agttcactct ttagcacagg tatttcaata gtgacaacgg attttcggga
    11521 gggcgccgtg tgagcgacag cgaacccagc ccgtaagagc aaatagtccg atagagtgct
    11581 aaaattgcga aagcaatttt aaaaacgaaa tgaaggacta tgcgtacccg aataatccaa
    11641 gaagcaagcg agcagtagat agtgtgcgac tgcgcaccct tttttagatt gcttcggtgg
    11701 gcaataattt tcaaagccga taatcgatgt ctcgcaatga cgtaaaacta gattcttcgc
    11761 catcgcacag aaagacaaat tacttgccct ctttctgctt ttcagccatt caaccaagct
    11821 aacaaatagg cggttttgca aatgcaaaac cgcctatttt aatattttat gctctaagca
    11881 tcgacatatt ctcttaaacg cttactgcga ttagggtgtc taagttttct aagtgctttt
    11941 gcctcgattt gtcttatacg ctcacgagta acgccaaaaa gctgtccgac ctcttctaat
    12001 gtgcgttgac gtccgtcatc cataccaaat cttagtctta aaacatcacg ctctcttgga
    12061 gaaagtgtac aaagtacatc tgctaaatct tctctcaaaa gttctgatgc aacggtcttt
    12121 acaggtgcat ctgcttcctt atcttcaata aaatcaccca gtcttgagtc ttcttctttg
    12181 ccaataggcg tttccaaaga caaaggctct tgagctattt taatgatttc tcttaatttc
    12241 ggtatgctca cattcatttc aatagctaat tcgtcttcgg tcggctttct ggaaagttcc
    12301 tgagcgagtc ttcttgtaac ttttttaagt ttattaatgg tttcaaccat gtggacaggg
    12361 attcttattg tacgagcctg atcggcgata gcgcgggtaa ttgcctgcct aatccaccat
    12421 gttgcataag ttgagaattt aaatcctctt tcatgatcga atttttcggc agctctgata
    12481 agtcccaggt taccttcttg aatcaagtca aggaaaagca ttcctcgtcc gacatatttt
    12541 ttggcgatac ttacaaccaa gcgcaagttt gcctgaacga gttttctttt tgcgatagca
    12601 ccgggtgtgc cgccctgtat tatttttctt gccaattcta tttcttcatt ggctgaaaga
    12661 agtttaattc gtccgatttc acgcagataa agcctaaccg gatcatcgac tgcgatgcct
    12721 ttaggtgctt caaaatcggt caaagtttct ccgacttctg ctgtttccat aacaaaaata
    12781 tcatcaaaac ccattttatt gcccattttt gcagatgaaa ctatatcttc aatgttatct
    12841 gtacctattt ctacatgtaa gtaaggggta ttttcatcag gaaccaagtc atccacgtga
    12901 tctttttctt catcggctga aattaaatct aaattttcat cttcttccat aatgctaata
    12961 actgaggtat ttgtttgtct ttttttcatt attatctccg aaatgtgtct ttaattttat
    13021 ctcttagttg aatttgtact tttagggctt ctgtatcatc atcatttacg tttttgtaca
    13081 aatttctcat ttcatttctt tcaatttccc gctgacattg ccgaatttta ttgatatttt
    13141 catcaataac ggccttaaag tctgtttctg ataggttatt aaaagtttct gatatgcata
    13201 ttaaatctgt aataattgcc tttatttcct cgtcttgaac aaaattaatg tataattgct
    13261 caattaatgc tttcacatta tttacggtac atattaattt gtcaattgta gttttaacaa
    13321 ttattaattt ttcatgatca aatttaatat tgctaatcat ttcgtttatc tgttgaaatg
    13381 tgaaatgact ttcatttatt aaataaaggc tcaataaatt tttttgcgct ttttccatga
    13441 tatttgaaga ttttgttaca attggattca aaacattatt tgacaatggt ttttgaaaat
    13501 caattttctt aatttccttt tgcagagcct cttcatcgat attcaaagct gaagccacca
    13561 tcttaatata ctctgaaaga ataattttat tctggatttc agctaaaatc ggaataattt
    13621 ctttaaccaa cttcaatttt tcggtcggag tttttgcttc gtttttctgt tttaagacct
    13681 gattaagctg aaaatcgagc aaaagcggag cgtgttcaag atattttgca tattcttttg
    13741 ctccggctgt tcttatgaat tcatcgggat ctttgccttc gggcggagta attacgcgga
    13801 tttcgcaggc atatttttca ttgcttgtgc tggaatagga ttcgtcaaac tgttttacgt
    13861 ttccaagccc cgtaaacgct tctttaataa tctctgcgcc tctttgagcc gctttaagcc
    13921 ctgcagaatc tgtgtcaaaa gaaagataaa tttttcttga ctttgaatat cttgaaataa
    13981 gctttatgtg ctctgttgta agtgacgttc cgcatgatgc aacgcaattt tcgattccgt
    14041 ttgcctgcgc ggagattaca tcaaagtagc cttccattac aatcacgcta tcagactgct
    14101 ttatggcttc ttttgcaaag taaagcccgt ataaaatctt gcttttgtta taaatcaaag
    14161 aatcagaaga atttaaatat ttggggttct ggccttcttc aagggcgcgc gcgccaaaag
    14221 caacaatgtc gcccatttca tcgtgaatag gaattataat tctatttctg aacctgtccg
    14281 tgtaagtccc ttttgaagat ttaaggacaa gtcctgcttt ttccaaaaca tcgtcggaaa
    14341 acttgtcttt aagcgaaacg taaaggtgat taggctcttt tgaagcaagc cccagcgaat
    14401 atctttttat aattttttcg tcaattcccc tgttgttaag ataagaaagt gcggttgagc
    14461 cctcagatga agcaaaaagt acttgttggt aaaattctgc cgcttttgcg cacgccttaa
    14521 tcatctggtc tttaacgtct ttatttttta cacccgaaaa agtcgatggc aattcaagcc
    14581 cgaactcgct tgcaagctct tttataagtt ctgaaaaatt tattccgcga gtttttatga
    14641 tgtagctgag cgcatcgccg ccttctccgc aaccaaagca cttataaatc ccttttgcgg
    14701 gattaacaga aaacgagggg gttttttctt tatgaaaagg gcacaatccc cagtaattcc
    14761 cgccggattt tttcaagata acgtcttttg aaacgacatc caaaatatcc aatctatcct
    14821 taatttgaga tacaacatcc tcaaacgtta cactactcat actcacctaa tccttcaata
    14881 acattttaac atcaataatt ttttctcaca ccctacacta agcctaattt ttgttaattt
    14941 ttgtaaatta caacgccttt aaattaggcg acgcaaaaag cgaatttgaa aaagaaccga
    15001 ataacttcca agtttgagca taaaaatcat taataaacag ggcgggtttt cgtgaaacga
    15061 aaacccgccg ctttttagta tttgaaatat ttaattatta aaataaaatc cgtctttttt
    15121 cattctatca atgacttcat aaatattttc atcgctgcag acgattgaaa ttctaaaaaa
    15181 tccttcgccg tatttcccaa agccgtcgcc gggaacggct ataatgccgg aggttctcat
    15241 caaatcgtcg gtaaattctt ttgatgtttt atatcttggc ggaataggca gccacagata
    15301 aaaagttgca tttggaatgt taatattgtc aatgtcccaa cctaattctg acaacccttt
    15361 tataaaaata tcctgtttct ttttaaaccc tgcatttgct tttttaatgt attcttcacc
    15421 ctcttttgag ttcaagactt cagatgccgc tttttgcaag gctttaaaaa tccctgtatc
    15481 aatcgtggtt ttaagcttac cgaaaatcga aaccgcctcg gaattaccgc aaacccaacc
    15541 caaacgccaa cctgtcatcg cataaggttt tgaaaagctg aaaaattcaa ccgcaatatc
    15601 ttttgcgcct tcaagttcta aaacactgtg cgggcggtca ttttcgtcaa aatacatatc
    15661 cgtataagcc gcatctgata taagcaaaat attgtgtttt ttgcaaaatt caaccaattt
    15721 tttaaaataa tcaagcgtag ctgtcgcgcc aagcggattg ttagggtaat ttatcaccaa
    15781 cgctttaatt tttttctcat cgtaaccctc ttttttaagc tgcgccaaaa cttcttccat
    15841 atcgggcatg taattgttct cataagtcag agggattgaa tatcccaccc cgcctgaaac
    15901 ctttatcatt tctttgtaag atgcgtatcc gggatcggga attaagataa tatcgcgctc
    15961 aaattcgtct tttttggccg taatcaaagc cctgataaaa tttgccaaac cttcttttga
    16021 accgataagc gaaaaaactt ctgttttaga ctctaaatca accccgaagc gctctttcat
    16081 cctttttgca acggcttcca gataaaaagc ttcgcctttt gggcttgaat aagagtggat
    16141 tccttcaaca tcaagcgcat cttttaattt gtcaataaca aattgaggag gattatcaac
    16201 aggcgcgccc aaagacaatg ctatcgggct tctgcctttt gaaatcaaat caggcttaat
    16261 acgagccact tcctcagaaa tcttaaacat aatgtatgtt tctaaggtct gaatataatc
    16321 gttaaaaaat tttttcattt tcacctccac aattatattt tattataaaa aaataacatg
    16381 gtataattgt ggggttaaaa tcaagctgtc atgctgaatt tacaaatcct cttgtttccg
    16441 aagcgaagcg aggattacaa gggagggtgt cgcttgcgac gtat
//
LOCUS       DAOQAU010000037        16226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_3809_length_16226_cov_6.980706, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000037 DAOQAU010000000
VERSION     DAOQAU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 16226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16226
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3809_length_16226_cov_6.980706"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKI94_06205"
     CDS             <1..541
                     /locus_tag="PKI94_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26367.1"
                     /translation="IIMAGCSILGGITGGAIFDKKENFKAKCREGIIQMGGNILIPLA
                     CVGGGSELYDKFLKQKVMTKFKLVGPKAEIPGAIVALITLSAAMFFGNKTTNFINEEI
                     YHIRDDRGVKFADLSGHLDDTCLALSKASKDSIISKVASRIIPLALLVCGYSSGVMQE
                     WPPEARITRKVDSKFKKQV"
     gene            573..1067
                     /locus_tag="PKI94_06210"
     CDS             573..1067
                     /locus_tag="PKI94_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26368.1"
                     /translation="MKVQFIGLQSHNLVNRRKKSNTKISTPLMTDNDKNLYNPSFSSG
                     GYGIALRNARCGRLRILERTHEEMIDMAESLHEYSPNIILSIATVYKWLGFATDKPKT
                     EIAYKKLSEAIQEVIEKKSKILAQIENNTGDLSALKNELDRIAEKVKKSYHEYDLFIE
                     NSGV"
     gene            1150..2295
                     /gene="proB"
                     /locus_tag="PKI94_06215"
     CDS             1150..2295
                     /gene="proB"
                     /locus_tag="PKI94_06215"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNW26369.1"
                     /translation="MDRSLISNTNRVVIKFGTNILTNDEGDVSLSRVYSFIEDVSKLR
                     KEGKEVILVTSGAVGLGKSKLGLESTDATALKQACAAVGQGRLMSIYEAGFDKYGLAV
                     AQILLTEDDFSQRKRYLSLRMTLNKLLELKVIPIINQNDTVSTIELDELYDDMQVCFS
                     DNDKLSALVASELDADLLVILSDVEGLYDSNPKTNSNAKIIPVVENVTDEILNLGEGA
                     SSGGRGGMRTKLEAARLVTRSGGTVLVANGKTPSVINQIFSENEIGTIFLPHQELLSG
                     KKRWIGYATNILGKIIVNYGAKKAILEKESSLLPIGVVEIRNEFKKGEVVSILDENEK
                     EIARGMVNYNSDDCKKLIGAHSDDISKILGYKNYDAVITRDNITVLE"
     gene            2298..3539
                     /locus_tag="PKI94_06220"
     CDS             2298..3539
                     /locus_tag="PKI94_06220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNW26370.1"
                     /translation="MENIVKKTKEASLKLVNLPIEARNYALEMIANSLDLHQKQIFDA
                     NQKDLQEAQKLVESGEISQAVFDRLKLDENKMRDMIAGVRDVIKLDNPINKCLLARSL
                     DEGLTLYKISCPIGVLGVIFEARPDVIVQISALAIKSSNAVILKGGKEAIKTNKAIFE
                     VITDALDCIKDFPKNVINQVYSREDVAQMLEMEGYIDLIIPRGGNSLVKFVQENTKIP
                     VLGHADGICHIFVDESADFEMAKNIVIDAKTQYPSACNSVETLLINNDFKYSDELLEA
                     LKNVEIKLIDNPESWAFEYGDKTLAYKFVSDVTEAIEHINKYGSGHTDCIISENFESV
                     EKFLNEVDSAGVYHNVSTRFADGFRYGFGAEVGISTNKTHARGPVGLEGLTIYKYKLY
                     GTGHVVKDYAQGLKQFHHSDL"
     gene            complement(3543..5822)
                     /locus_tag="PKI94_06225"
     CDS             complement(3543..5822)
                     /locus_tag="PKI94_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26371.1"
                     /translation="MEKMLQKYSCELEKKLFENVFEKTPDSIKNLNLIDLSNKDEFEF
                     VLKKEHLQPYDKHDNPEGLNLWDWFENYEKEAQVSTAGIRGPQNILFPQDTRFPINLI
                     GITLATLAKALVAKEKYPNKRLVKLAGREVRYNSEFYLEAITRIQAAQGIKTLVSENK
                     QTIPIWLASFLILKLGLLGGEYITSSHGISVKNATKDLNFQGSQYLPEESAEFVAKIK
                     EIFKQTEKEGTYSIKFSKADDKLIDEKAMKEMNDGVDLYVEYLQTGVARKFNMELIQN
                     LKNKIVIENVGGSAYRTLSRVLDKLEISEKFDWLNTQEDPFFHSIGKSDIDNKGNKGF
                     YDYSVDATVVAKNKEGKNFFPVIESLDYDSKLKKYPVNTVILITDPDHDRLTIVQIEN
                     SDKIDILKTAGIDYVRLSKDRVLAVYTANQAFLMLMDFRLKKLKAMGVFQNYPRFIIK
                     TTASSLSWDEWASNNGISVINVPVGFKEIANIMKKVELQIKNAPDKEVIISDVFANKI
                     NLGIEPRLLFAGEESGGMIVGPGKLIESATGYEAVAMREKSATEAIIIASALTSQLEK
                     ENKTLSQHLLDVFETNKITGKYDTRADISYYNESEPDIKKLKEAKKEGEKKRTNNDLF
                     FLTLAIGIACKEITFEQAKEILADAFCQLDFSNLTKIKFAGDGTYMEFEDKFIEIRPS
                     GTDAKTKAYAAGLDKKELEMYTQILGNYSGDRTKMYKELIPEDFYQNSKDEAMKFYLH
                     FVNKDLNNGGFTIPEYKEF"
     gene            6002..6625
                     /locus_tag="PKI94_06230"
     CDS             6002..6625
                     /locus_tag="PKI94_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26372.1"
                     /translation="MKIYSVNPVNNFKLNSNMGCNSPRKAVVFGNLPVSADRHANGAY
                     KVFDLEYLKLIKNKKVDGKDYLVEHPLFYVYPQRHIVLLDGAKVDTVYMYENEGHGTL
                     QVSTKYFYDNKNISNSIPTSVGEAIFKAEREAVREMKTFYRGNPYEGTDSYDTLMIDS
                     KSHKSLSGASYRKDGTLDAITKYKDGKAVEILEYDETGKIVINTIQL"
     gene            6666..7274
                     /locus_tag="PKI94_06235"
     CDS             6666..7274
                     /locus_tag="PKI94_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26373.1"
                     /translation="MKILFNAFNNSFYQTNNVQAKKSPQVCASLSKDLFVKNSSHIHF
                     KGERLETDNKSQLLEIVNAEQLLERLNDNGKVEGKDYLSDLEDKASVNAYRHLVILDG
                     GSPCVIYNFDAANSKLVFKEIFKYPVIEDRDEEIENKPVDIIKDYSCAFAYARTNAES
                     MKTIYSKEDGGCSVMFFDFNSKRIPEIQDYDKYGKLKMITRC"
     gene            7375..8052
                     /locus_tag="PKI94_06240"
     CDS             7375..8052
                     /locus_tag="PKI94_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW26374.1"
                     /translation="MREKADKDEAVKKYMTRTVENYNCWRRIWQWLVTKFWYGLSYRF
                     IYRLEVYGQENIPKTNDYIVVGNHLSTLDPPLVCHVLPHPVAYMGKKELFYHPFLKVM
                     LDWLGTFAVDREKVGLSTIKTAKSIRNTKKWVLGLFPQGTREKGNEIRKVTKGFVGLA
                     KATGCNILPIGITGTDKPTKIPFTGKIIVRIGPIIELSDDTDDMFNKWIKAIEELTGF
                     TYVPEEV"
     gene            8078..8770
                     /locus_tag="PKI94_06245"
     CDS             8078..8770
                     /locus_tag="PKI94_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW26375.1"
                     /translation="MKKKEEKNYARRYAHEYHFLRAFFQFFAISISYPYFKIYYNFKP
                     EGRQNIPKSGRYIFAPNHISMFDPLFVSCTILRPMVYMAKRELFEKGHNLEWWVKRLG
                     AFSVNREKPELATFKTIKDVFKTGWPLVIFPEGGIKDFKRIENVYKGFATIAKNFEAD
                     IIPVSIGGFEGYKKKPFAQTVRVKVCPPISHKQSEEEIIKQWAKQICDETGFENLVVT
                     GNEAEKIPEAVS"
     gene            complement(8772..9266)
                     /locus_tag="PKI94_06250"
     CDS             complement(8772..9266)
                     /locus_tag="PKI94_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26376.1"
                     /translation="MVNTEQTKNLKRIFFEFFGMRENWKWKRNILGLLMVHAPEYPTY
                     LIPAYTQAKATGNAKELKKGEGLRGFLTGMLEGLAVGALVSGEKLKIKEMIPFIVLGA
                     GLQFFSSQFFPWAAEKVGKHIYLKNRKKSISKQITNISNNKIKPVFQNFKGQNIYPKN
                     SIKI"
     gene            9439..9765
                     /gene="ssb"
                     /locus_tag="PKI94_06255"
     CDS             9439..9765
                     /gene="ssb"
                     /locus_tag="PKI94_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNW26377.1"
                     /translation="MNTAVIVGRAGQDPEIKYFESGKVKTTFSLAVNRWDNKTKSEVT
                     DWFNIDVWDKQAEFAGEYVKKGRLVAIDGRIAANKWTDASGAQKERFNIIANNVRLLG
                     SKRDEN"
     gene            9769..>10556
                     /locus_tag="PKI94_06260"
     CDS             9769..>10556
                     /locus_tag="PKI94_06260"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HNW26378.1"
                     /translation="MIISNLIGIIADDLTGANDTALQFHLRGANTQIVLDYEAFLQNI
                     KNTQVWAIATETRNLPVDVAYEKVKEVTKACIEKLNLEFFYKKVDSTIRGNIAAETLA
                     MLDVLEWDAAIIVPAFPAEGRTTVGGYHLLKGIPVERTEVARDVHSPIFESHIPTLLK
                     SQLPVEKKDLVGLIELSTIMKGAGPILIKLNELIKQGKKLIVADAVSITDIEQIVLSI
                     NKSDYNILPAGSAACAQVLGNVWLPELKNQHITKTIPELPKLIIS"
     assembly_gap    10557..10656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10657..10842
                     /locus_tag="PKI94_06265"
     CDS             <10657..10842
                     /locus_tag="PKI94_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HNW26379.1"
                     /translation="GETSYKCCRAIDSLQLQLVDEVAPSIALSIDHKAQWIVTKSGNL
                     GNSNTLIDIVNYFEQHE"
     gene            10835..11776
                     /gene="pdxA"
                     /locus_tag="PKI94_06270"
     CDS             10835..11776
                     /gene="pdxA"
                     /locus_tag="PKI94_06270"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNW26380.1"
                     /translation="MNKQKKIAITTGDANGIGAEITIKALNKLDLPIENVVLISNSKI
                     LNFYGKLKKKYPVIEIDFDMKNLLAGKPTAQSGDFAFRALKKACQMVKNKQIDAIVTA
                     PVSKETMHLAGHKFSGQTEVLQKFLAQKGQSAQMLFVAEDFRVLLLTRHLPLREVSID
                     KKDFIKKIKILDEFFKSKLKIRAPKFGLCAFNPHAGEGGILGREEIEILLPAVKALRK
                     QGVDITEPLPADTLFVKAAKAYLNRKKNPYDCYIANYHDQGLIPIKALAFEKTVNMTI
                     GLDVIRTSPSHGTAFDIAGKNLADESSMTEAIKQALI"
     gene            complement(11773..12096)
                     /locus_tag="PKI94_06275"
     CDS             complement(11773..12096)
                     /locus_tag="PKI94_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26381.1"
                     /translation="MYEYDIEHEDKELKKVGLELMFLTPEKYASEKGITAVELSKLVQ
                     LPVETVKKKLCILREKEIVLSKGINPKYWLFNEYNFQRMDEDDDVFQLLCSFDDVDFD
                     RFFQY"
     gene            complement(12144..13514)
                     /locus_tag="PKI94_06280"
     CDS             complement(12144..13514)
                     /locus_tag="PKI94_06280"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNW26382.1"
                     /translation="MREEHDLLGKLEISECDYSGIHTKRAEENFAVSGYKIDEDFIKA
                     YGIVKLACIKTAFAIEKTDSAKEKAIFQACEEMADGKLNQYIKVDAFQGGAGTSLNMN
                     VNEVIANRALEIMGEKKGNYEIISPLNDINKFQSTNDTFPTALKIAAIYKIRTLEEAV
                     ISLQEALQKKEADFYGLVKIGRTELHEAVLTTLGKEFSAYAEAISRDRWRIYKCEERL
                     RVVNLGGTAIGTGVGAPKKYIFEVVETLRDLTSLGLARAENLVENTQNADVFSEVSGI
                     LKAHASNLIKIVSDLKLLEAFDEIYLPAVQAGSSIMPGKVNPVIPEMVSQIGLKVIGN
                     DVIISHASALGQLELNQNMPLIAFTLLESLNMLINTNKIFTKKCINSITANEEGLKLK
                     VNSSTAILTALLPKLGYDKLSEVSEKIKETNLGVKEYILKENLMSEEDFDYLISAENI
                     LAINEI"
     gene            complement(13516..14730)
                     /gene="hydF"
                     /locus_tag="PKI94_06285"
     CDS             complement(13516..14730)
                     /gene="hydF"
                     /locus_tag="PKI94_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNW26383.1"
                     /translation="MKALKSMRLHIGIFGKRNVGKSSILNALVEQDISIVSETAGTTT
                     DPVEKSMELLPLGPVLFVDTAGIDDEGALGKQRVEKTKKVIDRIDLAIIICDSRGFDE
                     FEEKLVEEFNIRKTPFIVVINKIDENKLIDLSTFQLFNPIKTSALTKEGLNELKQSII
                     KNVPENFFAQSSIISDLIPPGEVAVLVVPIDKEAPAGRLILPQVQTIRDLLDNDSIAI
                     VTKERELKKTLDNLKTPPAIVVTDSQAFLKVSADVPKSIPLTSFSILFARFKGELNEF
                     VKGTMAIEKLKSGDKILICESCTHHAIGDDIGRVKIPRWLTQYTGKKLTFEHYAGHDF
                     PENIKDYALIIHCGACMTNRREILSRIFKAKQADIPITNYGLTIAYSLGIFERALGPF
                     KSAKLIYDELKE"
     gene            15242..15315
                     /locus_tag="PKI94_06290"
     tRNA            15242..15315
                     /locus_tag="PKI94_06290"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15276..15278,aa:Ile,seq:gat)
     gene            15542..15625
                     /locus_tag="PKI94_06295"
     tRNA            15542..15625
                     /locus_tag="PKI94_06295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15575..15577,aa:Leu,seq:tag)
     gene            15855..15927
                     /locus_tag="PKI94_06300"
     tRNA            15855..15927
                     /locus_tag="PKI94_06300"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15888..15890,aa:Ala,seq:ggc)
     gene            15965..>16226
                     /locus_tag="PKI94_06305"
     CDS             15965..>16226
                     /locus_tag="PKI94_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW26384.1"
                     /translation="MTEISDIKSDICILGGGPAGYFTAIRASQLGASVVLIEQGEIGG
                     TCLNRGCIPTKALLKTAEVADLLNKSNEFGIQISEPSFIIQNS"
BASE COUNT         5079 a         2676 c         3157 g         5214 t
ORIGIN      
        1 aattattatg gcgggatgtt caattttagg aggcattacc ggtggggcaa tttttgataa
       61 aaaagaaaat tttaaagcaa aatgcaggga aggcataata caaatgggcg gaaatattct
      121 tatacctctt gcttgtgtcg gtggaggcag tgagctatat gataagtttt taaaacaaaa
      181 agttatgacg aagtttaaat tagttggtcc aaaagcggaa atacccggtg caattgttgc
      241 attaattacc ctctcggcgg cgatgttttt cggaaataaa acaaccaact ttataaatga
      301 agaaatttat catattagag atgatagagg agttaaattt gctgatttat caggacactt
      361 ggatgatact tgtctggctt tatccaaggc aagcaaggac tcaattataa gtaaagtggc
      421 tagccgtata attcctcttg cacttttggt ttgcggatat tcaagtgggg ttatgcagga
      481 gtggccgcct gaggcaagaa ttacaagaaa agtcgattct aaatttaaaa aacaagttta
      541 aaaacattct aaaactaaat ataggaggat atatgaaagt acaatttata ggtttacaaa
      601 gtcataattt agtaaataga agaaaaaaat caaatacgaa aatttctact ccactgatga
      661 cagataatga taaaaatttg tataatccga gtttttcttc agggggatac gggattgcac
      721 ttcgtaatgc aagatgtgga cgattgagga ttttagaacg gacgcatgaa gaaatgatag
      781 atatggcaga atctcttcat gaatattcgc caaatataat tctttctatt gccacagttt
      841 ataaatggtt aggttttgct actgataagc caaaaactga aatcgcatat aaaaaattgt
      901 ctgaagctat acaagaagta attgaaaaaa aatcaaaaat tttagcgcaa attgaaaata
      961 acactggcga tttaagtgcc cttaaaaatg aattagatcg gatagcggag aaggtaaaga
     1021 aatcttatca tgaatatgac ttatttatag aaaatagcgg agtttaatac gtttttataa
     1081 aattattaac ctttaagttt aaattctaat ggcttgaaag aaagtgtgat tttatgtata
     1141 atcactccta tggacagaag tttgatttct aatacaaaca gagttgttat aaaattcgga
     1201 accaatattc taaccaatga tgagggtgat gtttctcttt caagggttta ttcttttatt
     1261 gaagatgttt caaaattaag aaaagaaggg aaagaagtta ttcttgtaac gtcaggcgct
     1321 gtcgggcttg gtaaaagcaa attgggactt gaatctacag atgcgactgc gcttaagcag
     1381 gcatgtgcgg ctgtaggaca gggacgtttg atgtcaattt atgaagcagg ttttgacaaa
     1441 tacgggcttg ctgttgcgca aattcttctt acagaagacg atttttctca aagaaaaaga
     1501 tatttgagtt taagaatgac attaaataag cttcttgagt tgaaagttat tccgataatc
     1561 aaccaaaatg atacggtatc aaccattgaa cttgatgaat tatacgatga catgcaagtt
     1621 tgtttttccg ataacgacaa gctttcagcg cttgtcgcaa gcgaacttga cgcagatttg
     1681 ctggtgattt tatctgacgt ggaaggactt tacgattcaa accccaaaac gaatagcaat
     1741 gctaaaatta ttcctgttgt tgaaaacgtt accgacgaga ttttgaactt gggcgaaggt
     1801 gcttcatcag gcggcagggg cgggatgaga acaaaattgg aagctgcaag acttgttacg
     1861 cgctcaggcg gaactgtttt ggtcgcaaac ggaaaaacac cttctgttat taaccaaatt
     1921 ttttcagaaa atgaaatcgg gacaatattt ttgcctcatc aagagcttct ttcgggcaaa
     1981 aaacgttgga ttggttacgc gacaaatatt ttgggtaaaa tcatagtaaa ttacggagct
     2041 aaaaaagcta ttcttgaaaa agaatcaagc cttttgccca ttggcgttgt tgaaataaga
     2101 aacgaattta aaaagggcga agtagtttcg attttggacg aaaacgaaaa agaaatcgca
     2161 cgcggaatgg ttaattacaa ttcagatgac tgcaaaaaac ttataggcgc acactcggat
     2221 gacatttcaa aaattttggg ctacaaaaat tacgatgctg ttataacacg cgacaatata
     2281 actgttttgg agtaattatg gaaaatatag ttaaaaagac taaagaagca tccttaaagc
     2341 ttgtaaattt gcccattgaa gcaagaaatt acgcccttga gatgattgca aactcgcttg
     2401 acttgcacca aaaacaaatt tttgacgcta atcaaaaaga tttgcaggaa gctcaaaaac
     2461 ttgttgaaag cggcgagatt tcgcaagcgg tttttgaccg ccttaaactt gatgaaaaca
     2521 aaatgcggga tatgattgca ggagtcagag atgttatcaa attagataat ccgattaaca
     2581 aatgcctgct tgcaaggtcc cttgatgaag gacttactct gtataaaatt tcttgtccta
     2641 tcggggtttt aggggtaatt tttgaggcaa gacccgatgt catagttcaa attagcgcac
     2701 ttgcgataaa gtcttcaaac gccgttatct taaaaggcgg aaaagaagca ataaaaacta
     2761 acaaagccat ctttgaagtc ataacagatg ctttggactg cataaaagat ttccccaaaa
     2821 atgttataaa tcaggtttat tcaagagaag atgttgccca aatgcttgaa atggaaggtt
     2881 atattgattt aattattccg cgtggcggaa attcacttgt aaaatttgtt caagaaaaca
     2941 caaaaattcc cgtgctcggt catgctgacg gaatttgcca cattttcgtc gatgaaagtg
     3001 ccgattttga aatggcaaaa aacattgtaa ttgatgccaa aacccaatat cccagtgcct
     3061 gtaattccgt cgaaacactt ttaattaata atgattttaa atattctgat gaactgcttg
     3121 aagctttaaa aaatgttgaa atcaagctta ttgataaccc tgaaagttgg gcttttgaat
     3181 acggcgacaa aacccttgcc tataaatttg tttcggatgt aacagaagcg attgaacata
     3241 ttaacaaata cggttcaggg cacacggact gtatcataag cgaaaatttt gaaagcgttg
     3301 aaaaattttt gaatgaagtt gattcggcgg gagtttatca caatgtttca acgcgctttg
     3361 cagacggttt cagatacggt ttcggggcag aggtgggtat ttccaccaac aaaacccacg
     3421 cccgcggtcc tgtgggctta gaagggctta cgatttataa atacaaactc tacggcaccg
     3481 gacatgtcgt aaaagattac gcacaaggct taaaacagtt tcaccacagc gatctatagg
     3541 ttttaaaatt ctttatattc aggtatagtg aaacccccgt tatttaggtc tttgtttaca
     3601 aaatgcaagt aaaatttcat tgcttcatct tttgagtttt gataaaaatc ttccggaata
     3661 agctctttgt acatctttgt tctgtcgccc gaataattcc ctaaaatctg tgtgtacatt
     3721 tcgagttctt ttttgtctaa acctgcggca taagcctttg tttttgcatc tgttcccgac
     3781 ggtcttattt ctatgaattt gtcttcaaat tccatataag tcccgtcacc tgcaaatttt
     3841 attttggtta aattcgaaaa atcaagttga cagaaagcat ctgccaaaat ttcttttgct
     3901 tgttcaaaag ttatttcttt acaggcaata ccgattgcaa gagtcagaaa aaaaaggtcg
     3961 ttatttgtac gttttttctc gccttctttt tttgcttctt ttaatttttt aatgtcgggt
     4021 tctgactcgt tgtagtaaga aatatctgct cttgtgtcat atttacccgt aattttattt
     4081 gtttcaaaaa catcgagaag atgttgggaa agagttttat tttctttttc aagctgggaa
     4141 gtcagggcag aagctattat aatggcttct gtagcacttt tttctctcat tgcaacagct
     4201 tcgtagccgg tcgcggattc tattagtttg cccgggccga caatcattcc gccggattct
     4261 tcgcctgcaa aaagcagtcg gggttctatc cctaaattta ttttatttgc aaaaacatct
     4321 gatataataa cctctttgtc aggggcgttt ttaatctgaa gttcgacttt tttcattata
     4381 ttggcaattt ctttaaatcc tacgggtaca tttattacac ttatgccgtt atttgaagcc
     4441 cattcatccc aggaaagaga agacgcggtg gtcttaatta taaatctcgg gtaattttga
     4501 aaaacaccca ttgctttaag tttttttaaa cgaaaatcca ttaacattaa aaaagcctga
     4561 ttagcagtat aaacagctaa tactctatct ttagaaagcc ttacgtaatc aatgcccgct
     4621 gtcttaagaa tatcaatttt atcagaattt tcaatttgaa caatagtcag cctgtcatga
     4681 tcggggtctg ttatcaaaat aacggtattt acaggatatt tttttaattt gctgtcgtag
     4741 tctaaacttt caattacggg aaaaaagttt tttccttctt tgttttttgc aacaactgtt
     4801 gcatcaaccg aataatcgta aaatcctttg ttgcctttgt tgtcgatatc tgatttccct
     4861 atcgaatgaa aaaaaggatc ttcttgcgtg ttaagccagt caaatttttc tgatatttca
     4921 agtttatcta aaaccctgct taaagtacga taggcacttc ctccaacatt ttcaatcact
     4981 attttatttt ttaagttttg aataagctcc atattgaact ttcttgcaac acccgtctga
     5041 aggtattcaa catataaatc aactccgtca ttcatttctt tcatagcttt ttcgtcgatt
     5101 aatttatcgt ctgcttttga aaatttaata ctataagtgc cttctttttc tgtttgttta
     5161 aaaatttcct ttatttttgc tacaaattct gcggattctt cgggcaaata ttggctgcct
     5221 tgaaaattta ggtcttttgt cgcattttta acagaaattc cgtgacttga ggttatgtat
     5281 tcacccccta aaagcccgag ttttaaaatc aaaaatgatg caagccagat aggaattgtt
     5341 tgcttatttt ctgaaaccag agttttaatt ccttgagcag cttgaattct tgtaatagct
     5401 tccaaataaa attcagagtt atatcttact tctctgcctg caagttttac gagtcgtttg
     5461 ttaggatatt tttctttagc cacaagtgct tttgccaatg tcgcaagagt tattcctata
     5521 aggttaattg ggaaccttgt gtcctgcgga aacaaaatgt tttgaggtcc tcttattcct
     5581 gctgttgaaa cttgagcttc tttttcatag ttttcaaacc agtcccaaag gttgagtcct
     5641 tcagggttgt cgtgtttgtc gtaaggctgc agatgctctt ttttaaggac aaattcaaat
     5701 tcatccttat tgcttaggtc aatcaaatta aggtttttga tgctgtcggg tgttttttca
     5761 aaaacatttt caaatagttt tttttcaagc tcacaagaat atttttgcag cattttttcc
     5821 atttaaaact cctttttaag actcacttcg tgttttttgt ctaagtatag cataaagaat
     5881 acgtttttaa aatccttcat agcaccgtta agtgcttttt atatgtaaca tttttttaac
     5941 aaaatagcaa aagaatttat tatgcgtttt attctattgt tattaaagca aggacattgt
     6001 aatgaaaata tattcggtta acccggtaaa taattttaaa ttaaactcaa atatggggtg
     6061 caattcacct cgcaaagccg tcgtttttgg taatttacct gtaagtgcgg accgacacgc
     6121 caacggtgct tataaagttt ttgatcttga atatcttaag ttgattaaaa ataaaaaagt
     6181 agatggtaaa gattatttgg ttgagcaccc tctattttat gtttatcctc aaaggcatat
     6241 agttttgctt gacggggcca aggttgatac tgtttatatg tatgaaaatg aaggacacgg
     6301 gactttacag gttagtacaa aatatttcta cgataataaa aatatttcaa attcgattcc
     6361 aacgtcagta ggtgaagcta tttttaaagc agaacgtgaa gccgtgcgtg aaatgaagac
     6421 tttttacaga ggcaatcctt atgaaggaac tgattcgtac gatactttga tgattgattc
     6481 caaatctcac aaatctcttt caggtgcatc ttacagaaaa gacggaactt tggatgccat
     6541 aactaaatat aaagacggaa aagccgttga aatacttgaa tatgatgaaa caggaaaaat
     6601 agtcataaat acaattcaac tttaaaaatt aatagtatat aggtcatttt ttaggagtta
     6661 agataatgaa aatattattt aatgcgttta acaattcttt ttatcagaca aacaatgtac
     6721 aagctaaaaa aagcccgcaa gtttgcgctt ctttaagcaa ggatttattt gtaaaaaatt
     6781 cttctcatat ccactttaaa ggtgaaaggc ttgaaactga taataagagt caattgcttg
     6841 aaattgttaa tgcagaacaa ctgcttgaaa ggctcaatga taacgggaaa gttgaaggca
     6901 aagattattt gagcgattta gaggacaagg cttctgttaa cgcatatagg catttggtaa
     6961 ttttagacgg cggcagtcct tgtgtcattt ataattttga cgctgctaac tccaaattgg
     7021 tttttaaaga aatttttaaa tatcctgtta ttgaagacag ggatgaagaa attgaaaata
     7081 agcctgtaga tattattaaa gattatagtt gtgcttttgc ttatgccagg acaaatgcgg
     7141 agagtatgaa aacaatttac tctaaagaag acggcggctg ttcggtgatg ttttttgatt
     7201 ttaattcaaa aagaataccc gaaatacaag attatgataa atatggcaaa ctgaaaatga
     7261 ttacacgctg ctagatttat atagaagatg cagaaaaatt aaaaattttg cgcagtttga
     7321 tatggaaaaa tttctttctt ttgtatataa ttgatttagg aaagaggatt tgttgtgaga
     7381 gaaaaagcag ataaagacga agctgttaaa aaatatatga caagaacagt tgaaaattac
     7441 aactgttgga ggcgtatttg gcagtggctt gtcaccaagt tttggtatgg tttgagttat
     7501 aggtttattt acagacttga agtctatggt caggaaaaca ttccaaagac aaatgactac
     7561 attgttgtgg gaaaccacct ttcaacgctt gatcctccgc tggtgtgcca tgtgcttccg
     7621 catcctgttg cttatatggg gaaaaaagag cttttttatc atccgttttt aaaagtgatg
     7681 cttgattggc tcgggacttt tgcagttgat cgagaaaagg tagggctttc aacgattaag
     7741 actgctaaat ccattcggaa tacaaaaaaa tgggtattgg ggctttttcc gcaaggaaca
     7801 agagagaaag ggaatgaaat tcgcaaggtt acaaagggat ttgtcgggct tgctaaagca
     7861 accggctgta atattttgcc tatcgggatt acaggcacag ataagcctac aaaaatccct
     7921 tttacgggaa aaattatcgt tagaataggt cctattattg agcttagcga tgatacagat
     7981 gacatgttca acaaatggat taaagcaatt gaggaattaa ccggctttac ttatgtgccg
     8041 gaagaagttt aaattttaaa atactcggag gttaagaatg aaaaagaaag aagaaaagaa
     8101 ctatgcaaga agatatgctc acgagtacca ttttttaaga gcattttttc agttttttgc
     8161 tatttcgatt tcttatccgt attttaaaat ttattataac tttaagcctg aaggcaggca
     8221 gaatataccc aaaagcggca gatatatttt tgcaccaaat cacatttcaa tgtttgatcc
     8281 gctttttgta tcgtgcacaa ttttgagacc aatggtttat atggcaaaaa gagaactttt
     8341 tgaaaaagga cataatcttg aatggtgggt caaaaggctt ggggcatttt ctgtcaatcg
     8401 tgaaaaacct gaactggcaa catttaaaac cattaaagat gtttttaaga caggttggcc
     8461 gcttgttatt tttcccgaag gcgggataaa agattttaag cggattgaaa atgtttataa
     8521 aggatttgct actattgcaa aaaactttga agccgatatt attcctgttt caataggagg
     8581 atttgaaggg tataagaaaa aaccttttgc gcagaccgta agagtaaaag tttgtccgcc
     8641 aatttcacat aaacaaagcg aggaagaaat tataaaacaa tgggcaaaac aaatttgcga
     8701 tgaaacggga tttgaaaatc ttgttgttac aggaaacgaa gcagaaaaaa tacccgaagc
     8761 cgttagttaa atcatatttt tattgaattt ttcgggtata tattttgtcc tttaaagttt
     8821 tgaaaaacag gtttaatttt attatttgaa atattagtaa tttgttttga aatggatttt
     8881 tttcgatttt ttaaatagat gtgttttccg actttttccg ctgcccaagg aaaaaattgg
     8941 ctggaaaaaa attgaagtcc tgctccaagt acaataaaag gaatcatttc ttttatttta
     9001 agtttttctc ctgaaactaa tgccccgaca gcaagacctt caagcatgcc tgttaagaaa
     9061 ccacgtaatc cttctccttt ttttaattct ttagcgttac ctgtcgcttt tgcctgggta
     9121 taggcaggga ttaagtaagt agggtattca ggtgcgtgaa ccataagtaa tccgagaatg
     9181 tttcgtttcc acttccagtt ttccctcatt ccgaaaaatt caaaaaagat tctcttaaga
     9241 tttttagttt gttctgtatt taccatgttt taacccaatt tatgatagta ttttaaaaat
     9301 cgaagataaa aaaatatgct atttttttta aaaattttaa aaaaaaatta acattactat
     9361 acataattaa ttatttttta tggcaaatta ttcgggttta ttgtaaaatt caggaataat
     9421 taaaaaaggg gatattatat gaatacagca gtaattgtcg gcagagcagg gcaagatcct
     9481 gaaattaaat attttgaatc aggaaaagtt aaaaccactt tttctttagc ggtaaaccga
     9541 tgggataata aaaccaaatc agaagttacc gactggttta atatcgatgt gtgggataaa
     9601 caagctgaat ttgcgggtga atatgttaaa aaaggccgct tggttgcaat agacggcaga
     9661 attgctgcaa acaaatggac tgacgcttca ggagctcaaa aggaacggtt taacattatt
     9721 gcaaataatg taaggttatt aggttcaaaa cgagacgaaa attaatttat gattatttct
     9781 aatttaatag gaattattgc agacgatctt acaggcgcaa atgatactgc gttacagttt
     9841 catttaaggg gcgcaaatac tcagattgtg cttgattatg aagctttttt gcagaatata
     9901 aaaaatacac aagtttgggc aattgccact gaaacaagaa atttgccggt agatgtcgct
     9961 tatgaaaaag taaaagaagt tacaaaagcc tgtattgaaa aattaaactt agaatttttc
    10021 tataaaaaag ttgattctac aatccgcgga aatattgcgg cagaaaccct tgctatgctt
    10081 gatgtcttgg aatgggatgc ggcaattatt gttcctgcgt ttcctgcaga aggcagaaca
    10141 acagtaggcg gctaccatct tttaaaagga attcctgttg aaagaacgga agtcgcaaga
    10201 gatgttcatt ctcccatttt tgaatctcat atcccgacgc ttttaaaaag tcagttgcct
    10261 gttgaaaaaa aagatttggt cggcttaatt gagctttcaa ctattatgaa aggcgcaggt
    10321 cctatcctta ttaaacttaa tgaattgata aaacaaggta aaaagcttat agtggcagat
    10381 gctgtttcca taacagatat tgagcaaatt gtactttcaa taaataaatc cgattataat
    10441 attttgcctg ccggctctgc cgcttgtgct caagttttgg gaaatgtttg gcttcctgag
    10501 cttaaaaatc aacatattac aaaaaccatt ccggagcttc caaagcttat aatttcnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtg aaacttctta taagtgctgt
    10681 cgagctatag actcactcca gcttcagcta gtggatgaag ttgcaccttc gattgctctt
    10741 tccattgacc ataaagccca gtggatagta acaaaatccg gcaatttggg caattctaat
    10801 actcttattg atatcgtaaa ttattttgaa caacatgaat aaacaaaaaa aaatagctat
    10861 tacaacaggt gatgcaaacg gtattggggc tgaaattacc ataaaagcac tcaataaact
    10921 tgatttgcca attgaaaatg ttgttttgat ttcaaattca aaaattttaa atttttatgg
    10981 gaagttgaag aaaaaatatc ctgttattga aatagatttt gacatgaaaa atcttttagc
    11041 cggaaaacct accgcacaaa gcggcgattt tgccttcagg gctttaaaaa aagcctgcca
    11101 aatggtaaaa aataaacaaa ttgatgctat tgtcaccgct cctgtttcaa aagaaacaat
    11161 gcatctagcg gggcacaaat ttagcggtca gacagaagtg ctgcaaaagt ttttggcaca
    11221 aaaagggcaa tctgcacaga tgctttttgt cgcagaggat tttcgtgttt tgcttttgac
    11281 aagacacttg ccgcttaggg aagttagcat cgataaaaaa gattttatta aaaaaataaa
    11341 aattttagat gaatttttta aatcaaaatt aaaaattcgc gccccaaaat ttgggctttg
    11401 tgcttttaat ccacatgccg gcgagggcgg aattttgggg cgcgaagaaa tagagattct
    11461 tttgccagcg gttaaagctt taagaaagca aggggttgac attacagaac cgcttcctgc
    11521 tgatacgctt tttgtcaaag ccgcaaaggc ttacttgaac cgcaaaaaaa atccttacga
    11581 ttgttatatt gcaaattatc atgatcaggg gttaatcccc attaaagcat tggcatttga
    11641 aaaaacggtg aatatgacaa tagggcttga tgttataaga acttctccat ctcatggaac
    11701 cgcttttgat attgcaggca aaaatcttgc cgacgaatcg agtatgactg aagctataaa
    11761 gcaggcatta atttaatatt gaaaaaatct gtcaaaatct acatcatcaa aactgcataa
    11821 aagttgaaaa acatcatcgt cttcatccat tcgttgaaaa ttatactcgt taaaaagcca
    11881 gtattttggg tttattcctt tggatagaac aatttctttt tcccttaaga tacaaagttt
    11941 tttctttact gtttcaaccg gcagttgaac gagttttgaa agttcgacag ctgtaatccc
    12001 cttttctgaa gcatattttt caggggtcaa aaacattaat tccagcccga ctttttttaa
    12061 ttctttatct tcatgctcaa tgtcatattc gtacattttt atatattagc agtaagaagg
    12121 gcttttgtta tcgtattaac ggattaaatc tcgttgattg caagaatatt ttcggcagaa
    12181 atcagataat caaaatcttc ttcgctcatt aaattttctt tcaaaatgta ttctttaact
    12241 cctagatttg tttcctttat tttttctgaa acctcagaaa gcttgtcata accgagtttt
    12301 ggcaaaagtg ctgttaaaat agccgtactt gaatttactt ttagctttaa gccttcttcg
    12361 tttgcagtta tcgaatttat acattttttt gtaaaaattt tgttggtatt aatgagcata
    12421 tttaagcttt caagcagggt aaaagcaata agcggcatat tttggtttaa ttcaagctgc
    12481 cccaaagcac ttgcgtgaga aattatgacg tcattaccta ttacttttaa accgatttga
    12541 gaaaccattt cggggataac cggattgact ttgccaggca ttatggatga gccggcttgt
    12601 acagcaggaa gatagatttc atcaaaagct tccagaagtt ttaaatcact tacaatttta
    12661 attaaatttg atgcgtgtgc ttttaaaata cctgatactt cgctgaaaac gtcggcattt
    12721 tgtgtgtttt cgacaagatt ttccgctctt gcaagcccta gtgaagttaa gtcgcgcagg
    12781 gtttcaacaa cttcaaaaat atatttttta ggagccccga cgcctgtgcc tatggcggtt
    12841 cctccgaggt taactactct gagcctttct tcacatttat aaattcgcca cctgtcgcgc
    12901 gaaatagctt cagcgtatgc cgaaaattct tttccgagtg tcgttaagac tgcctcgtgc
    12961 agttcggtgc ggccgatttt tacgagcccg taaaaatcgg cctctttttt ctgcagggct
    13021 tcttgaaggc ttattactgc ttcttcaagc gttcttattt tatatattgc tgcaatttta
    13081 agcgcggtag ggaacgtgtc gtttgttgac tggaatttgt taatgtcgtt aagcggtgag
    13141 atgatttcat aatttccctt tttttcgccc attatttcaa gtgctctgtt tgcgattact
    13201 tcatttacgt tcatgttcag ggaagttcct gcaccgcctt gaaaggcatc gacttttatg
    13261 tactggttta attttccgtc agccatttct tcacaggctt gaaagattgc tttttctttt
    13321 gcggagtcgg ttttttctat tgcaaaagcg gtttttatgc aggcaagttt tacaattccg
    13381 taggctttta tgaagtcttc atctatttta tagcctgaga ctgcaaaatt ttcttcggca
    13441 cgttttgtgt ggattccgct gtaatcacat tcggatattt caagttttcc aagtaagtcg
    13501 tgttcttcgc gcattttatt cctttaattc gtcgtaaata agttttgcgc ttttgaaggg
    13561 tccaagcgct ctttcaaata tgcccaaaga gtaggcgatt gtcaatccgt aatttgtaat
    13621 aggtatgtcg gcttgttttg ctttgaagat tcttgaaagg atttccctcc ggtttgtcat
    13681 gcatgcgccg cagtggatta ttagggcgta atctttaatg ttttcaggga aatcatgtcc
    13741 tgcgtagtgc tcaaatgtta gttttttacc ggtatattga gtaagccagc gagggatttt
    13801 taccctgccg atatcatcgc caatagcgtg gtgggtgcag gattcgcaga ttagaatttt
    13861 gtcgcctgat ttgagttttt caatagccat tgtgcctttg acaaattcgt ttaattcgcc
    13921 tttgaatcgg gcaaaaagta tcgaaaatga agtcaaggga atactttttg gaacatcggc
    13981 agaaactttt aaaaatgctt gcgaatcggt tactacaata gcaggaggag tcttgagatt
    14041 gtccaaagtt ttttttaatt ctcgttcttt tgttacgatt gcgatagaat cgttgtccag
    14101 caaatctctt atggtctgaa cctgtggcaa aatgagtctt cccgcgggtg cttctttgtc
    14161 aatcgggaca accaatacgg caacttcacc gggcgggatt aagtcggaaa taatacttga
    14221 ttgtgcaaag aaattttccg gcacgttttt gattatactt tgttttaatt cgtttaaacc
    14281 ttctttggtg agtgctgaag tttttatagg gttgaaaagt tgaaaagtcg ataagtcgat
    14341 aagtttattt tcgtcaattt tgtttatgac aacaatgaaa ggggtttttc taatattaaa
    14401 ttcttcaacc agtttttctt caaattcatc aaatcctctt gaatcgcaga taataatcgc
    14461 caaatcaatt ctgtcgataa ctttttttgt tttttcgacg cgttgtttgc ccagtgcgcc
    14521 ttcatcgtct atgcctgcgg tgtcaacaaa gagcacaggg ccgagcggca aaagttccat
    14581 agatttttcg acagggtctg ttgtagttcc tgcggtttca gaaactattg aaatgtcttg
    14641 ttcgacaagt gcgtttagaa tacttgattt gccgacgttt cttttaccga aaattcctat
    14701 atgcaaacgc attgatttaa gtgctttcat agtgcctcct gtactgatga ttatactata
    14761 aacttacgga taaaacctct tgattttgag aaatgtctat gatagtctag aaattgaccg
    14821 aaatattgaa aaatgaataa attcattttg gctgaacagg tgaatagttt actaaggtga
    14881 ataagtgaat aggttaataa gtgaataagt tcaaattgtt gcttcgcaac ggaatataat
    14941 taataagact caaaaacttc tcacttctca ctggtaaaat ggctattagc tcaggtggct
    15001 agagcactct gccgaacaaa acgattaagt atcgttttga gagaggcctg ataagggtca
    15061 gcccgataga acaagaattg aaaacagaat acaaaaaata taataagggc tattagctca
    15121 ggtaagcccg aaggcagaat gcccgaagaa aaaagattaa gtatcttttt tcaaggagaa
    15181 gtttcgagag ggacgcgcca cctgagttaa taaatggttg aaaatataat aattaaaatg
    15241 ggggctatta gctcaggtgg ctagagcgca cccctgataa gggtgaggtc cctggttcga
    15301 gtccaggatg gcccaaatta agcggctcag acttttaagt ctgagccgct taattttgaa
    15361 aataactagt ctcaaatagg atttcaccct tatcagggat gctcaaagac accgaaggtg
    15421 gcgtatgagc actctgccga agggaaaggt actaaatgta cctttccctg agaggccctg
    15481 ataatggtga gtttaaatag tcctttgacg gaggattttt ttcatatata atagttttaa
    15541 tgggcgagtg gtgaaatggt agacacgcta gtcttaggaa ctagtgccgc aaggtatagg
    15601 agttcgagtc tcctcccgcc caccataaaa ataaagataa cttttgttag ctttattttt
    15661 ataaagcgag atagagacga gaattcctca caaaacgaag ttttgtcgga gttcgagcgg
    15721 gagcgagcag agggcgaagg tcttttgcgg caacgggcaa agcccgtgaa agcaaaaacc
    15781 gcagacccgt ttaacgcgag cgaccaagac agtctcctcc cgctcaattt ctttttgtgt
    15841 tatatttatt ctatggggca ttggcgcagt tggtagcgcg caacgctggc agcgttgagg
    15901 tcaggggttc gaccccccta tgctccaaat atatcctaat tttacaaaag ttgaggataa
    15961 atttatgacc gaaattagtg atattaaatc agatatatgc attctgggag gtggtcctgc
    16021 aggctatttt actgcaatca gggcttcgca gcttggtgcg tcggtcgtct taatcgagca
    16081 gggcgaaatc ggaggaacat gtcttaacag aggctgtatt cctacaaaag cacttcttaa
    16141 aactgctgaa gttgctgatt tgcttaataa atcaaatgaa ttcggcatac aaatttccga
    16201 accctctttt ataatccaaa actcaa
//
LOCUS       DAOQAU010000038        15888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_3950_length_15888_cov_6.825554, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000038 DAOQAU010000000
VERSION     DAOQAU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15888
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3950_length_15888_cov_6.825554"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..495
                     /locus_tag="PKI94_06310"
     CDS             322..495
                     /locus_tag="PKI94_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26385.1"
                     /translation="MLKILKNFFNDDSTKVGLCGFNLQKPARQKLATESIFNSAQLPK
                     TFKTDFEKNVLLI"
     gene            593..1363
                     /locus_tag="PKI94_06315"
     CDS             593..1363
                     /locus_tag="PKI94_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26386.1"
                     /translation="MVNISKVGQTIFKAVEKSFPLGLDGISKIPPNLKFNISNPHLLE
                     ALPDHLITSDGIKFLSELNGKPLSKVAKFLDSVGGDFKTFSRAMEKYLKGAFQKIQKG
                     FDDEEFTKLYNKAFPTKKVPISVNEDAFLYLNGLPNNIGSRFDAHGMDKISVTNQLRQ
                     LNNLLTNGIDKSRNFFTAPLSCDPHLGAGLGTAGGHAYRSGSFIVVADKGKGLVKDGI
                     KHVIVNDAYYNIIDDLAAKFPDIKFVRADKAVDYFKQL"
     gene            complement(1390..1899)
                     /locus_tag="PKI94_06320"
     CDS             complement(1390..1899)
                     /locus_tag="PKI94_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNW26387.1"
                     /translation="MIEMRVMGIALDTRTGSPIVVLHDKDNRKALPIWIGSAEASAII
                     RKIENLAVSRPMTHDLIISIIEKTGYKLNRIEINDVEKETYYATLFLINDKEEVIEID
                     SRPSDAIAVAIRVDAPIFVTSNVLLSGSVSTDSGKDEEESKEFKSFIQSVKPSDFAKL
                     LKDNPESDQ"
     gene            complement(1981..3105)
                     /locus_tag="PKI94_06325"
     CDS             complement(1981..3105)
                     /locus_tag="PKI94_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNW26388.1"
                     /translation="MKKFFLILFLILGFALPAFCTQGEEILKSQVGKVLSVENLIENI
                     LPQSGGIKQIKQSVEVEVLTGEKKGDKIIVDNMLMGNPAYDINLKNGDRVILHAEKAQ
                     GGINFFIADKQRVNTLYFLSGLFCLLVLIVGKKRGLFSLLSIFFTLGLVFWALTPLIL
                     SGMNPILATVLVCIISTIGTMYLVGGLNYKSTSAILGTGLSLVFAGLMSLIVIKTASL
                     TGFSSEESLFLFTAHPNLNFTGVLASAMIIGALGALMDIGMSIASTVNELFENNRTMS
                     VRDLFDSGMNVGRDIIGTMANTLILVYLGGSLSLVLLSNNIDLQKFFNLNQVATEISA
                     ALIGSIALVLCVPATALIASFLVRKTKQKEVAQNSFDDII"
     gene            complement(3112..4185)
                     /gene="ruvB"
                     /locus_tag="PKI94_06330"
     CDS             complement(3112..4185)
                     /gene="ruvB"
                     /locus_tag="PKI94_06330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNW26389.1"
                     /translation="MSIFSDDDEKLGGKCPERCEDTIPNVTEFDRSFENNIRPKTFET
                     YIGQSSLKETLKISIEAAKKRELPLDHLLFYGPPGLGKTTLAGVIANEMGVDIKITSA
                     PALERPRDIIGILMSLQGGEILFIDEIHRLNRVAEEILYPAMEDFFLDMNTGKGQSVK
                     TLRIPVPKFTLIGATTKAGALSGPLRDRFGMVYRLEFYSPEELTQVAKRTAQILDIPI
                     TDEGAKAIAKRSRGTPRIANRLVKRVGDYALVKHDGEITEKIANESLDSLKIDDFGLD
                     STDRCLLKLIIEKFDGGPVGIETISAALGEDVRTIEDVCEPFLLQAGFLQRTPRGRKV
                     SPEALRYLGYKSKSMQQNLFENE"
     gene            4870..6057
                     /locus_tag="PKI94_06335"
     CDS             4870..6057
                     /locus_tag="PKI94_06335"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNW26390.1"
                     /translation="MKQSARLDKIPPYLFAQIDKKIAEAKAKGIDIISLGIGDPDTPT
                     IPTVVEAMHKAIDNPANHDYPPYNGTKEFRQASVKWMKERFNIELDADKEMLANIGSK
                     EAIAHVFFAFVDEGDYTLIPDPGYPVYKNATILAGGTPYSMPLLEENNFLPDFDKIPE
                     EIAKKSKLMFLNYPNNPTGAICDLEFYKKAVDFCKKYNILLCSDMAYSEMTYDGYVAP
                     SVLQIEGAKDIAIEFFSHSKSYNMTGWRVGFVAGNSEAIKALGTIKNNIDSGVFKAIQ
                     QAATKAYEAPKSQIDNLNKMYKERKDIMEKGLKELGWDIKPSKATFYLWLKVPKGYTS
                     EEFVTLMLEKAGVVVPPGTGYGEWGEGYFRIALTKDIQTLKKAFERMKKAGICYNMGA
                     IAK"
     gene            complement(6185..8809)
                     /gene="gyrA"
                     /locus_tag="PKI94_06340"
     CDS             complement(6185..8809)
                     /gene="gyrA"
                     /locus_tag="PKI94_06340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNW26391.1"
                     /translation="MSQQNMFQEGKIVPVNIREEMKRSYIDYAMSVIVGRALPDVRDG
                     LKPVHRRILFAMNELGMTPDKPYKKSARIVGEVLGKYHPHGDTAVYDSMVRMAQDFAT
                     RYQTIDGHGNFGSVDGDSAAAMRYTEARMHKITTSMLADIDCETVDFTPNFDGSLEEP
                     SVLPVRLPMLLLNGVSGIAVGMATNIPPHNLCEVVDGTIALIDNPDISIEELNTYIKG
                     PDFPTAATIIGLNDIKQAYATGRGSIKMSAVAGFEELPGGGGRQSRTAIVVTELPYQV
                     NKAMLIEKIAELVKDQKLQGISDLRDESDRDGMRVVIELKRDAKPDVVRNNLFKQTQL
                     ATSFGVNMLALVGKQPRLMNLYEVLSEFVEHRVEVITRRTIFFLKKAKMRAHILSGLL
                     IALGSLDEVIELIKKSKTTDDARLGLMSRFGLDTDQANAILEMQLRRLTGLEQDKIKA
                     EYDELLKKIKEYEAILADRQKVLNIIKEELLEDKEKYGDDRKTQILPEQGEMSIEDLT
                     PNTAMAVFITHQGYLKRISLDTFERQNRATRGKSGIKTKEDDDIQHFFTAMMHDKVLF
                     FSSKGTVYSLNVYDFPEGQRQAKGLPIVNVLPIEQDEKITAVVPVSAFSKDLNLIMLT
                     KGGYIKRIGLDNFANIRRNGIIAIGLEEGDGLNWVKLANSKDEIIIGTSCGMAIRFGV
                     SDLRPLGRSARGVNSMKLRTGDSIIGCDIVPRDYDADLLVVTSDGFGKRSKLSEFRAQ
                     NRGGIGLIATKFKSSASRLVALTIVEEKDEIMLVTANGIVTRIKAGDISRQGRPATGV
                     KAQNLTGNDLVMSVNKIVDPDDDDELEAKIAAGMERALQQQQLQPQQEPQAQEEIIEN
                     TLFDIEDLNSEVDEHDEE"
     gene            9035..10816
                     /locus_tag="PKI94_06345"
     CDS             9035..10816
                     /locus_tag="PKI94_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26392.1"
                     /translation="MEMVNLDNINDLIGEKEFEQAKNHLQNLLKQDGQNAEILKMLGL
                     CNINLGLFGEAKSNFETAVKYKNDDASSWFYLANCYDNLQEFAQAKNAYLEVIKLREN
                     FLDAYKNLSVIYLRTKEEDKAIELSQKALLITQEDYIFYYMIGTALLAKRQMSESIPY
                     LEHALELNPEHAQVHNNLGTAYLTTGDYQKSYDFFIKASKLDPKSSITYYNIGSILQI
                     QGKLKEACEFYEKAYNVEPIENYIMSLAFAEYKSEQYEKAIKHYKILVTHHPEKQNFQ
                     YNLACCYEMIGEYTYAIGILDQLILLNPKSTTMTQKLAGLYLKINQPLKAKEIYRKII
                     AQGTVSVDVYYEYALLCTKTGDISTAETILKKVLSLNPEFSLAHKDLGIIFLTKRLFD
                     YAKDEFEKALKLDEENPLILFEYANFLHATNDFQKAKEFYQKAKDKAPDSADVKTFSA
                     VNFIALNELENALECSESALKKFPEDAFILFVTGKVYYLMKNFDKAKWFLIKSYEKNR
                     NSETENLLALTYFELGEFEQANNIFEKLLKENPSNTNVLFNSAKCLIELSDTKSAQKQ
                     LKKILKIFPEFEEAKELLNEIKKGEKL"
     gene            10813..11217
                     /locus_tag="PKI94_06350"
     CDS             10813..11217
                     /locus_tag="PKI94_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26393.1"
                     /translation="MRTDFKEYLEKKNAQKAIDKIAKQFKDKKVVLYGAGFMASDLLR
                     NYDFSRLNIIGIADIKFQDDTEGDFYGYPKLGPYDLLETEFDLLLITTYDDEPVRDFF
                     NDDLFEGEEINFKVKTLVKMSLWDYIKQIMNA"
     gene            complement(11300..12121)
                     /locus_tag="PKI94_06355"
     CDS             complement(11300..12121)
                     /locus_tag="PKI94_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26394.1"
                     /translation="MDYADLNSIKEPAYRVGTSDDVEQKSKTVVSVDSESVKELESTI
                     NIEKLTYADLSLKKISAQISQDLALESDVMMGDLSMLWQGAATRSDIIKFAIYKLSNP
                     DADKPDEKSVKKVLQTVASMSTLLGAGMGNPVLSSGSFIGGNVLGIMSQDDKAINYKY
                     TKVNDADMIILVRKVDELQQQVVDKYYDYMTSRQMYDMSVKMVKQRHKNYQLSQNSSK
                     EVILITDAYYRDALDMQMKARSEFYSKRASLEQLVGNEVFKKFEADVSLREKEIK"
     gene            complement(12367..13188)
                     /locus_tag="PKI94_06360"
     CDS             complement(12367..13188)
                     /locus_tag="PKI94_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26395.1"
                     /translation="MRKFFLILLSIFVSSLSAFALSEVSLQNVQKQQIQVEKAPKIIK
                     GSLVISDEQMLDELIKLQKQKDMEDIENLWKATVANNKVIEFALKKLSTPESQRRIHS
                     SLMAKTLSAIISGGSFAPSFMGADPFIQTGSFAAGRIAQTLLNKKNIPQEIPLTDTEL
                     IELAGLVENLQDKIIDAYYNYKSCLNQVKDSRARLILYNKNYSKALDKGDILDIAISS
                     SLYDNMMLEEFYYMQNAKKYHLELQRLAGKKAVAKLNLYQYNFNAALFKDKGGKV"
     gene            13309..15009
                     /locus_tag="PKI94_06365"
     CDS             13309..15009
                     /locus_tag="PKI94_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNW26396.1"
                     /translation="MNFNTKIKNAFTLAEMMVVLLVLSIVMAAFLPVITTRTKADRSS
                     PWKYSQNNSDIYYGMGATQGVLIGATKFDPIYDTDNPKLAINMPLKGAAHIVFKENGT
                     RTGTMVLDQNHNIVIGSSPSFNKLTNAISIGETKINSDSVAIGQDAWTVENESVAIGS
                     TAHADAGANSSTVIGFQANSNGKESVSVGKNSGAYSDYAVALGSSATAGNSQSISIGA
                     FSMAGGVSSIAIGSLDKANQTSANDEYAIAIGPAAQATKKNAIAIGGLKELSNPGSAC
                     MSQSEGSIAIGGVSNVGGTAAIGLGYNSTVEKSNSAILIGTDSSINIADKAIIIGSVS
                     GIGSSATASIAIGSGVKISSSSTNSVAIGYGAQVSGGATNSVAIGPGVEVNMSNTIKL
                     GADNTYVVDTPGDLHAGGDAYISGFVNCRGVIPTSDKRLKNIKGEFKGGLNEIKKLEI
                     FNFTFKDDKQKTPMVGVMAQDLQKVFPDAVTKDKKGFLAIRQEDMFYSMVNAIKELDN
                     IFQGIIKDFKKFVCTVEKIEDKIEALIKVDQINSKKIKELEAKNKQLEERLAKLERSK
                     "
     gene            15177..>15888
                     /locus_tag="PKI94_06370"
     CDS             15177..>15888
                     /locus_tag="PKI94_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNW26397.1"
                     /translation="MYQESEYNSYSKIKRLSDEELESLWSQYLADKNDKKLRDTLIVQ
                     YIYLTRYVVGRVKVSLPPTFSVEDISSYGVEGLIDAIEKFSPDKGARFETYALMRIRG
                     NIIDKIRSQDFLPRSARRKIKEVKETQEQLRQKLGRMPTSAEIGEVLGLEKEKIDQIL
                     AEDTTITSIYDKKGSSEESLEIIDTIQDTHRLTPHEEMEEKDVKKELERALKKLPERE
                     RMIMVLYYHENMTLKEIGE"
BASE COUNT         5209 a         3042 c         2843 g         4794 t
ORIGIN      
        1 agttccccct ttacttttta atttttatcc ttaaacacat tatacaacaa ataaaagact
       61 aatgcacaag tctatttttt acaaaaataa ccttgtgcaa agtcttaaga cggctagtgt
      121 cgaatggcta aacggcactc ctcgtgccga ttttgccatt ctcacttttg taaggtcgac
      181 cactgcaaaa gtgagaaaaa acaaattgcg gatggcgagc tgaggcattc caaggcaaaa
      241 ttgcattttg cctgaatgcc gttaaacgcg agccaccaag aattctacac ttgtgcagtg
      301 gtcttaataa agggaaaata aatgctgaaa attttgaaaa acttttttaa cgacgacagt
      361 acaaaagtcg gactttgcgg ctttaacctt caaaaacctg cccgacaaaa acttgcaacg
      421 gaaagcattt ttaattcggc acaacttcca aaaaccttca aaaccgactt tgaaaaaaat
      481 gttttactga tttaataaat aatttttttt ctatatcaaa acttatatga agacaatttt
      541 ttgttttaaa ttgtttttat atattagtag gttatttaac ggttaaagtc aaatggtaaa
      601 tatttcaaaa gttggtcaaa caatattcaa agccgtagaa aaaagttttc ctttgggctt
      661 agacggaatt tccaaaattc cgcctaattt gaagttcaat atttcaaacc cacatctact
      721 ggaagcttta cctgaccact tgataacctc ggacggcata aagtttttgt ctgaactcaa
      781 cggcaagcca ctgtcaaaag ttgcgaaatt tttagactct gttggtgggg attttaaaac
      841 tttttcaaga gcaatggaaa aatatttaaa aggcgctttt caaaaaatac aaaaagggtt
      901 tgatgatgaa gaatttacaa aattgtacaa taaagctttt cccacaaaaa aagtacctat
      961 tagcgtaaat gaagatgctt ttttgtatct taacggattg cctaataata taggctcaag
     1021 gtttgatgct cacggaatgg acaaaataag tgttacaaat caattaagac aattaaataa
     1081 tcttcttacc aatggaattg acaaaagcag aaactttttt acagcccctt tgtcttgtga
     1141 ccctcatctt ggtgcgggct tgggaactgc aggcggtcat gcttacagat ccggttcttt
     1201 tattgtagtg gcagataaag gtaaaggttt agtgaaagac gggataaaac acgtaatagt
     1261 caatgatgcc tactacaata taatagacga tttagctgca aaatttcctg atataaaatt
     1321 tgtcagagca gacaaagctg ttgattactt taaacaacta taaaatctaa aaagaaatgc
     1381 atttacccct tactgatctg attcggggtt atcttttagg agttttgcaa aatctgaagg
     1441 ttttacgctc tgaataaaac ttttgaactc tttggattct tcctcatctt tgcccgaatc
     1501 ggttgaaaca gagccgctca acaaaacatt cgaagttaca aaaatcggcg cgtccaccct
     1561 tatagcaacg gcgatggcat cagacggtct tgaatctatt tcaattactt cttctttgtc
     1621 gtttatcaaa aacaaggtgg cataataagt ttctttttca acatcgttaa tctcaattct
     1681 gttgagttta taacctgttt tttcaataat gcttatgatt aaatcgtggg tcatcgggcg
     1741 cgaaaccgcc agattttcta tttttcttat aatggcactt gcttcagccg agcctatcca
     1801 tataggaagg gctttacggt tgtctttgtc atgcaaaacc acaattggtg aacctgttct
     1861 tgtatcaagg gcaatgccca ttactcgcat ttctatcatg gcttaacgtc ctttgttaaa
     1921 attattaatt attttgttct aaataaatga taaataggat tttttactat ttactattta
     1981 ctaaattata tcatcaaaac tgttttgtgc cacctctttt tgtttagttt ttctgactaa
     2041 aaaactggcg attaaggcag ttgcgggcac acataaaaca agtgctatac ttccaattaa
     2101 agctgccgag atttccgttg ctacctgatt aagattaaaa aacttctgca aatcaatatt
     2161 atttgacagt agaaccaaag aaagcgagcc gccaagatac actaaaatca aggtgtttgc
     2221 cattgtgcct attatatctc tgccgacatt catgccggaa tcaaacaaat ctcttacact
     2281 catcgttcga ttattttcaa aaagttcgtt aaccgtactt gcgatagaca ttcctatatc
     2341 cataagtgcg cccaaagcac cgataatcat agctgaggca agtactccgg taaaattcaa
     2401 attcggatgt gcggtaaaca aaaacaagct ttcctctgac gaaaatcctg tcaaacttgc
     2461 ggttttgatg acaattaaag acattaaccc tgcaaaaaca agacttagtc ccgttcctaa
     2521 aatcgcagat gtacttttgt aattaagccc cccgacaagg tacattgtac ctatagttga
     2581 aattatacac accaaaacag ttgccaatat gggattcatg ccagacagta taagaggtgt
     2641 taatgcccaa aatacaagtc ccagcgtaaa aaatatagac aaaaggctga aaagccctcg
     2701 ttttttgccc acaatcaaaa ctaaaagaca aaaaagcccc gataggaaat aaagtgtatt
     2761 aaccctttgt ttatcggcaa taaaaaagtt tatcccgcct tgcgcctttt ctgcatgcaa
     2821 aatcaccctg tcgccatttt ttaaatttat gtcgtaagct ggattcccca taagcatatt
     2881 atcaacaatt attttatcgc cttttttttc gccagttaaa acttcaactt caacgctctg
     2941 tttaatttgt tttattccgc cgctttgtgg caaaatattt tcaatcagat tttcaacgga
     3001 aagcacttta cctacttgag atttcaaaat ttcttcgccc tgagtgcaaa aagcgggtaa
     3061 ggcaaatcct aaaattaaaa ataaaatcaa aaaaaacttt ttcatattgc attactcgtt
     3121 ttcaaataaa ttttgttgca tactttttga tttatagccc aaatagcgca aggcttcggg
     3181 tgaaactttt cttccgcgcg gagttcgctg caaaaaaccc gcctgcaaaa gaaaaggctc
     3241 acaaacatct tcgatggttc tgacgtcttc gcccagtgcc gcagaaatgg tttcaattcc
     3301 aaccggtccg ccgtcaaatt tttctataat aagttttaac aagcacctgt cagtactgtc
     3361 taagccaaaa tcgtctattt ttaaactgtc gagagactcg tttgctattt tttcggttat
     3421 ttcaccgtcg tgtttcacaa gcgcatagtc tccgaccctt tttacaagcc tgttcgctat
     3481 tcttggagtt cctcttgacc ttttggcaat agcttttgcg ccctcatcag ttatgggaat
     3541 atccaaaatc tgtgctgttc tttttgccac ttgcgttaat tcttcgggag aataaaattc
     3601 cagtctatag accatgccaa acctgtctct taaaggacct gataaagcac ctgcctttgt
     3661 tgttgcacct atcagcgtga atttgggaac gggaattctc aaagtcttta cgctttgacc
     3721 ttttccggtg ttcatatcaa ggaaaaagtc ttccattgca gggtacaaaa tttcttctgc
     3781 taccctgttt aatcgatgaa tttcgtctat aaaaagaatt tctccgcctt gcaaggacat
     3841 caaaatccct attatatctc gcggccgctc aagtgcgggt gcggaagtta tttttatatc
     3901 cacgcccatt tcatttgcga taacccctgc aagagtggtt tttccaagtc cgggaggccc
     3961 gtaaaacaaa aggtgatcaa gcggaagctc gcgttttttt gcagcttcaa tcgaaatttt
     4021 taaggtttct tttaaagagc tttgcccgat ataagtttca aaagttttgg ggcggatgtt
     4081 gttttcaaaa cttctgtcaa attcggttac attcggtatt gtgtcctcac aacgttcagg
     4141 acacttaccc cccaattttt cgtcgtcatc agaaaaaata ctcataggtt aattttatca
     4201 tataattata ttttttggag gaaaattaat atttttttgc tacaatggtt atgtcattgc
     4261 aaacccttca aagtgaggca attcaaaaat attgtcatcc tgaatttatt tcaggatcta
     4321 tccaacttag aatgctggca aaatgttgat actattgaaa actgaaatta aaatgtgtac
     4381 aatccatgta taccgtaagg acatttttca caaaatcaaa gattttggaa aaagaagtca
     4441 aacaagttca gcatgacgtg ggttattttt tggaataatt ccgcattctc aatattctta
     4501 tttttattta caatatttaa tattgttcct ttttttgata caataaaaca aaagaacatt
     4561 gaaagaagaa taaagggtaa gccccgacct ccctcacaac aaccgtttag tgaagaaaac
     4621 gtgtgagaaa ggaggtgata cattgaatac caaattaatt aaaaaagccc tagcgattct
     4681 agagctttta attaaattga ttttggcgtc aatttaaggg gtgaagcagt tgtcttaagt
     4741 gtcttaatcg cttaaggcaa cttccctttg tttttattat aacatatttt gatattattt
     4801 caagtgaccc tccttgtgga gcaggaagtg ttttattgga ggtgttcaac ataaagaaag
     4861 aggttaaaca tgaaacaatc ggcacgtttg gataaaattc cgccttacct ttttgcccaa
     4921 atcgacaaga aaattgccga agcaaaagcc aaaggcattg acataataag tttgggaatc
     4981 ggtgatcccg acacccccac aattccaact gtagtagaag ctatgcacaa ggcaatcgat
     5041 aatcccgcaa accacgatta tccaccatat aacggaacaa aagaattcag gcaagcttct
     5101 gtaaaatgga tgaaagaacg cttcaatatt gaacttgacg cggataaaga aatgcttgca
     5161 aacataggtt caaaagaagc tatagcacat gttttctttg cgtttgtgga tgaaggcgac
     5221 tatactctta tccctgaccc cggttatccc gtttataaaa acgcaacaat tttagcaggc
     5281 ggaacccctt attctatgcc gcttttagaa gaaaataact tcctccccga ttttgacaaa
     5341 atccctgaag aaattgcaaa aaaatcaaaa ttaatgttct taaattaccc gaacaaccct
     5401 acaggtgcaa tttgtgattt agaattttac aaaaaagcag tagatttttg caaaaaatac
     5461 aatattttgc tctgctctga tatggcatac agcgaaatga cttatgatgg atatgtagca
     5521 ccttcagttt tacaaatcga aggtgcaaaa gatatcgcaa tagaattttt ctcccattca
     5581 aaatcataca acatgacagg ctggagagtc ggatttgtcg caggaaactc tgaagcaatc
     5641 aaagctcttg gcacaatcaa aaataacata gacagcggtg tatttaaagc aattcaacaa
     5701 gctgctacaa aagcttacga agctccaaaa tcccaaattg ataacttgaa taaaatgtat
     5761 aaggaacgaa aagatattat ggagaaagga ttaaaagaac ttggctggga cataaaacct
     5821 tcaaaagcaa ctttttacct gtggctgaaa gttccaaaag gctatacctc agaagaattt
     5881 gtgacactaa tgcttgaaaa agcaggggtt gtagtaccgc cgggcacagg ctacggagaa
     5941 tggggcgaag gttattttag aatagcactt acaaaagata tccaaacact caaaaaagca
     6001 ttcgaaagaa tgaaaaaagc gggaatttgc tataacatgg gtgctattgc aaaataaacc
     6061 gagttgaaaa gtcgaatagt cgataagttg aaaagttctt ttaaactttc ttgtcgttgc
     6121 gaggagcatt tatgcgacga agcaatccaa aaaaatctgt gggcaggtat gcccacaata
     6181 tatcttattc ttcatcatgt tcatcaactt cagaatttaa atcctcgata tcaaacaggg
     6241 tattttcaat aatttcttcc tgagcttgag gttcttgttg aggctgaagc tgttgctgct
     6301 gaagtgctct ttccatgcct gctgcaattt tagcttccag ctcatcatcg tcatcagggt
     6361 caactatttt attgacagac ataactaaat catttcctgt caaattttga gccttaacgc
     6421 ctgttgcagg tcttccctgt ctagagatat cgcctgcttt aatccttgtg acaataccgt
     6481 ttgcggttac aagcattatt tcatcttttt cttcaacgat agtaagtgct acaagccttg
     6541 aagccgaaga cttgaatttg gttgcaataa gccctattcc gcctctgttt tgggctctga
     6601 attcagagag tttagagcgt tttccaaagc cgtctgaagt taccacaagc aagtcagcat
     6661 catagtctct cggcacgata tcacagccga taatactgtc gcctgtgcga agtttcattg
     6721 aatttacgcc tcttgcgctt ctgccaagcg gtctgaggtc ggaaaccccg aatcttattg
     6781 ccataccgca acttgtaccg attatgattt cgtcttttga atttgcaagt ttaacccaat
     6841 tcagaccgtc gccttcttca agtccgattg ctatgattcc gtttctgcgg atgtttgcaa
     6901 aattatcaag tccgattctt tttatgtaac cgccttttgt gagcataata agattcaaat
     6961 cttttgaaaa agcacttacg ggaacaaccg ctgtaatctt ttcatcctgt tctataggaa
     7021 gaacatttac tataggcaaa ccttttgcct gtctttgtcc ttcggggaaa tcataaacat
     7081 tcaggctgta aaccgttcct ttagaactga agaaaagaac tttatcgtgc atcattgcgg
     7141 taaagaagtg ctgaatgtcg tcgtcttctt ttgttttaat tcctgattta cctcttgttg
     7201 cgcggttttg acgctcaaat gtgtcaagag aaatccgctt caagtaccct tgatgagtta
     7261 taaatacagc cattgcagta ttaggtgtca aatcttcaat actcatttcg ccttgctcgg
     7321 gcaaaatttg agtttttcta tcatcgccgt atttttcttt gtcttcaagc aattcttctt
     7381 taataatatt aaggactttt tgcctgtcgg ctaagattgc ttcatattct ttaatttttt
     7441 tgagaagttc gtcgtattcg gctttaattt tatcctgttc aagtcctgtc aaacgtctta
     7501 gctgcatttc aaggattgca tttgcctgat cagtgtccag tccgaatctt gacattaagc
     7561 ccaatcgggc atcatcggtt gtttttgatt ttttaattaa ttctataact tcatcaagtg
     7621 agcccagtgc aatcaaaagt cctgacaaaa tgtgtgctct catttttgct tttttgagga
     7681 agaaaatagt tcttcttgta ataacttcaa ccctgtgctc gacaaattcg ctcaaaactt
     7741 cgtataaatt cattaagcgg ggctgcttgc cgacaagtgc aagcatatta actccgaaag
     7801 aagttgcaag ttgtgtttgt ttgaataaat tatttcttac cacatcaggt tttgcatcac
     7861 gcttaagttc aataaccacg cgcatgccat ctctgtctga ttcatccctt aagtcagaaa
     7921 ttccttgtaa tttttgatct ttaacaagct ctgcgatttt ttcaataagc attgccttgt
     7981 tgacctgata aggaagttcg gtaactacaa ttgccgttct gctctgtctt ccgccacctc
     8041 ccggtaactc ttcaaatcct gcaaccgcag acatctttat tgagccacga cctgtagcat
     8101 aagcttgttt aatgtcgttt aatcctataa tagttgccgc cgtagggaaa tcaggccctt
     8161 taatatatgt atttaattct tcaatagaaa tatcaggatt gtcaattaaa gcaatggttc
     8221 cgtcaacaac ctcgcaaagg ttatggggcg gaatatttgt tgccatacca acggcaattc
     8281 ccgaaactcc gtttaataaa agcataggaa gccttacagg caaaacactt ggttcttcaa
     8341 gcgagccgtc aaagttgggt gtaaaatcaa ctgtttcaca gtcaatatcg gcaagcatag
     8401 aagttgtaat tttatgcatt ctggcttcgg tataacgcat tgcagcagca gaatcaccgt
     8461 caacagagcc gaaatttcca tgcccgtcta ttgtttgata tctggtcgca aaatcctgcg
     8521 ccatccgcac catagaatca taaaccgcag tatcaccgtg agggtgatat ttacccagaa
     8581 cttctcctac gatacgcgct gactttttgt aaggcttgtc aggcgtcatc cccagttcgt
     8641 tcatcgcgaa caagatcctt ctgtgaacgg gctttaagcc gtctctgaca tcgggaagcg
     8701 cacgacccac aattacgctc attgcataat ctatgtaaga acgcttcatt tcctctctta
     8761 tattaacggg aacaatcttc ccttcctgaa acatgttttg ttgactcaaa atctctaccc
     8821 cctgctatgc tattattgta tcacaagcaa ggtttaatgc aagaagaaag taaagaaagt
     8881 tgaaaagtcg aatagtcgaa tagtcgaata gttgaaaagt taaaaagtta aatttattat
     8941 aattgcactt attcacttat ttacctattc acttctattt accatttacc atttactatt
     9001 tactatttaa ccctgtttat aatataattt tcctatggaa atggttaatt tggataacat
     9061 aaacgattta atcggagaaa aagagtttga acaggcaaaa aatcatctgc aaaacctgct
     9121 caaacaagac ggtcaaaatg ccgaaatctt aaagatgcta gggctttgta acattaattt
     9181 ggggttattc ggtgaggcaa aaagtaattt tgaaaccgcc gttaaataca aaaacgatga
     9241 cgcctcaagc tggttttatc ttgcaaactg ctatgataat ttgcaagaat ttgcccaagc
     9301 aaaaaacgcc taccttgaag tcataaaact cagagaaaac tttttggatg cttataaaaa
     9361 ccttagcgtg atttacttaa ggacaaaaga agaagacaaa gccattgaat tgtcacaaaa
     9421 ggcacttttg ataacgcaag aagactatat attttattac atgataggaa cagcacttct
     9481 ggccaaaaga caaatgtcag aaagtattcc ctatcttgaa catgctctgg aacttaaccc
     9541 tgagcatgcc caagttcaca ataatctcgg aacagcatat ctgacgacag gtgattacca
     9601 aaaatcttac gattttttta ttaaagcaag caaacttgac cctaaaagtt ctataactta
     9661 ctacaatata ggctcaattt tgcaaattca aggaaaattg aaagaagcat gcgagtttta
     9721 tgaaaaagcc tacaatgttg aacccattga aaactatatt atgtcacttg cttttgccga
     9781 atataaatca gaacagtatg aaaaagcaat aaaacattat aaaatactag tcactcacca
     9841 cccggaaaaa caaaattttc aatataatct ggcatgctgt tatgaaatga ttggcgaata
     9901 cacttatgcc ataggcattt tagaccaatt aatactttta aaccccaaat ctacaaccat
     9961 gacgcaaaaa cttgcagggc tttatttaaa aataaaccag ccgcttaaag caaaagaaat
    10021 ttacagaaaa attatcgcac aaggaactgt ttccgttgat gtttattatg aatacgccct
    10081 tttatgtaca aaaacaggtg atattagcac tgcagaaact attttgaaaa aagtcctttc
    10141 tttaaatccg gaattttctc ttgcacacaa agatttgggt ataatattct taacaaaaag
    10201 actttttgac tatgccaaag acgagtttga aaaagcactt aaactagatg aagaaaatcc
    10261 tctgatactt tttgaatacg caaattttct tcatgccaca aacgattttc aaaaagcaaa
    10321 agaattttat caaaaagcaa aagataaagc tcccgattct gccgatgtga aaactttcag
    10381 cgcagttaat tttatcgccc ttaacgaact tgaaaacgcg cttgaatgct ctgaatcagc
    10441 tttgaaaaaa tttcccgaag atgcttttat actttttgtt acgggcaaag tctactattt
    10501 gatgaaaaac tttgacaaag caaaatggtt tttgataaaa tcgtatgaaa agaaccgaaa
    10561 ttcagaaacg gaaaaccttt tggctcttac atactttgaa cttggtgagt ttgaacaagc
    10621 aaataacatt tttgaaaaac ttttgaaaga aaatccctca aatacaaacg ttctttttaa
    10681 tagtgcaaag tgcctgatag aattgtccga tacaaaatca gcccaaaaac aacttaaaaa
    10741 aatacttaaa atatttcccg aattcgagga agcaaaagaa ttactcaatg aaataaaaaa
    10801 aggagagaaa ttatgagaac cgattttaaa gaatatttag aaaagaaaaa cgcccaaaaa
    10861 gccatcgaca aaatagcaaa acaatttaaa gacaaaaaag tcgttctcta cggcgcaggc
    10921 tttatggcaa gcgacctgtt gagaaattac gatttttcaa ggctgaacat aatcggaatt
    10981 gccgacatca aatttcagga tgacacggaa ggagatttct acggttaccc taaattaggt
    11041 ccttatgatc ttttggaaac agaatttgac cttcttttga taacaactta cgatgatgag
    11101 cctgttagag acttctttaa cgatgatctt tttgaaggcg aagaaatcaa tttcaaagtc
    11161 aaaactcttg taaaaatgag tctttgggat tatataaaac aaattatgaa cgcttaattt
    11221 tgtttaaaat caaagagggg cggtgttttt ttaaaaaaac accgcccctc ttaacatctc
    11281 gccttttatt ttatataaat tatttaatct ctttttctct taagctgaca tcagcttcaa
    11341 attttttaaa aacttcgttt ccgaccaatt gttctaaaga agcgcgtttt gaatagaatt
    11401 cggagcgtgc tttcatctgc atatcaagag catcgcggta ataagcatct gttatcaaaa
    11461 ttacttcttt ggaagaattt tgagaaagct gataattctt atgacgctgt ttaaccattt
    11521 ttacgctcat gtcatacatt tggcgagaag tcatataatc ataatacttg tcaacaactt
    11581 gttgctgaag ttcatcgact ttgcgcacaa gaattatcat atccgcatcg tttacttttg
    11641 tgtatttgta attaatcgcc ttgtcatcct gcgacataat cccaagaaca tttccgccga
    11701 taaaagagcc gcttgataaa acaggattac ccattcctgc acccaaaagc gttgacatgc
    11761 ttgcaactgt ctgcaaaact tttttaacag atttttcatc cggtttatcg gcatcagggt
    11821 ttgaaagctt gtaaattgca aattttataa tatcgcttct tgtggcagca ccctgccaga
    11881 gcattgataa atcacccatc atcacatcag attccaaagc caaatcctga ctgatttgtg
    11941 ccgaaatctt tttcaggctc aaatcggcat aagtaagctt ttctatattt atagtacttt
    12001 ctaattcttt aaccgattca gaatcaacag aaacaaccgt tttagatttt tgttcaacat
    12061 catctgaagt cccgactctg tatgccggtt cttttatgga attcagatcg gcgtaatcca
    12121 tggcaaaaca aggcgaattt aagaatataa aagtaaatag taaatagtaa atagtaaata
    12181 gatgtaaata gttgaatgac tgaaaaactt tttgggcttt tatctcttcc cctcgcccct
    12241 tgagggagag ggaaaaattt tcaatgcgag aaacgagcat tagaaaaatg ggtgaggggt
    12301 attttggatt gcttcggact aaagtcctcg caacgacgcg agaataaata gtaaatagta
    12361 aatttttcac actttacccc ctttgtcttt aaaaagggcg gcgttaaaat tgtattgata
    12421 caaattcagc ttagcaacag cttttttgcc cgcaagcctt tgcagttcaa ggtggtactt
    12481 cttggcattt tgcatataat aaaattcttc cagcatcata ttgtcataaa gcgaagaaga
    12541 aatcgcaatg tctaaaatgt cacctttatc aagtgctttt gaataatttt tgttgtataa
    12601 aataagcctt gcacgcgaat ctttaacctg gttaagacag cttttataat tgtaataagc
    12661 gtcaataatt ttatcctgaa gattttcgac aagtcccgca agttcgatta attcggtgtc
    12721 ggtgagagga atttcctgag ggatgttctt tttgttcaaa agtgtttggg caattcttcc
    12781 tgcagcaaat gaacctgttt ggataaaagg atcagcaccc ataaaagaag gtgcaaaaga
    12841 gcctccgctt attatggcgg acaaggtttt tgccattaat gacgagtgaa ttcttcgctg
    12901 gctttcgggc gtggaaagct ttttaagggc aaattcaatt accttattat ttgcaaccgt
    12961 tgccttccag agattttcta tatcttccat atctttttgt ttttgaagct ttatgagttc
    13021 atcaagcatc tgctcatcag aaatcaccaa tgagcctttt ataatcttcg gtgccttttc
    13081 aacttgaatt tgctgttttt gcacattttg cagagaaact tcgctaagcg cgaaagcgct
    13141 caggcttgaa acaaaaatac tcaacaagat taaaaaaaac ttcctcataa tatttttata
    13201 gcacagagct tgagcaaaaa caaatcaact atataaacgg cttaacatta ttgcattcgt
    13261 gagaaaataa agttataatt atattaattt acaatgtgga gaatagctat gaattttaat
    13321 acaaaaatta aaaatgcatt tactcttgca gagatgatgg ttgtactttt ggttttgagc
    13381 atagttatgg cggcattttt gccggtgata actacccgca caaaggcaga ccgttcaagc
    13441 ccatggaaat actctcaaaa caattccgac atatattatg gcatgggggc tactcaaggg
    13501 gttttaatag gtgcaacaaa atttgacccc atatatgata cagataatcc taagcttgca
    13561 ataaatatgc cattaaaagg agccgctcat attgttttca aagaaaacgg aacaagaaca
    13621 gggacgatgg ttttagacca gaaccataat atagtgatag gaagcagccc aagttttaat
    13681 aaattaacaa atgcaatttc cataggcgaa acaaaaataa acagtgattc ggttgctata
    13741 ggacaagacg cctggacagt ggaaaatgaa tctgtcgcca taggttcaac ggcacatgct
    13801 gatgcaggcg caaactccag cacagtcata ggatttcaag caaattcaaa tggcaaagaa
    13861 agtgtatccg tcggaaaaaa ctcaggcgca tattcagact atgctgtcgc attgggctcc
    13921 agcgctacag cgggtaactc tcaaagtatt tctattggcg cattttctat ggctggaggc
    13981 gtcagcagca tagcgatagg ctctttggat aaagccaatc aaacaagcgc caatgacgaa
    14041 tatgcaatag caatagggcc tgccgctcaa gccaccaaaa aaaatgctat agcaataggc
    14101 ggactgaaag aactgtcaaa ccccggcagt gcctgcatgt ctcaaagtga gggttctata
    14161 gcaatcggcg gagtgtcaaa tgtcggagga acagctgcaa taggtctggg ttataattct
    14221 actgttgaaa aatcaaattc agcaatatta ataggaacgg atagttctat aaacattgcc
    14281 gataaggcaa taataatagg tagtgtctca ggaataggct ccagtgctac agcttctatt
    14341 gccataggtt ctggagttaa aataagttcg tcatcaacca atagtgttgc cataggttat
    14401 ggagcgcaag taagtggagg tgcaaccaat agtgtcgcca taggaccggg cgttgaggta
    14461 aatatgagta atactattaa gcttggggcg gataatactt atgttgttga tacccccggg
    14521 gatttacatg cgggtggaga tgcttacata tcagggtttg ttaattgcag gggggttatt
    14581 ccaacatcag acaaaagact taaaaacata aaaggtgaat ttaaaggtgg gttaaatgaa
    14641 attaaaaaat tagaaatttt taattttacc tttaaagatg ataaacaaaa aacacctatg
    14701 gtcggagtta tggcacagga tttgcaaaaa gtttttcctg atgcggttac aaaggacaaa
    14761 aaaggattcc ttgctatcag acaagaagac atgttctact caatggtcaa tgccataaaa
    14821 gaacttgaca atatttttca gggaattatc aaagatttta aaaaatttgt ttgcacagta
    14881 gaaaaaattg aagataaaat cgaagcgtta atcaaagttg accaaattaa ttccaaaaaa
    14941 ataaaagagc ttgaagctaa gaataaacag cttgaagaaa gacttgcaaa acttgaaaga
    15001 agtaaatagg aaatagtaaa tgtttaaaga aaaaagggag cgactttgct ttgcaaagtc
    15061 gctccctttt ttcttttctt gtttcacatt aacaaagtcg tctatataca tgatgtcaga
    15121 ttatccagat ttactacaat tattataggt atctcgtagg taaatcagga ttattgatgt
    15181 atcaagaatc tgaatacaat tcttatagca aaattaaaag gctctccgat gaagagctcg
    15241 aaagtttatg gtctcaatat ttagccgaca aaaacgataa aaaattacga gatacactta
    15301 tcgttcaata tatttacctt acaagatacg ttgtagggcg ggtaaaagtc tcattgccgc
    15361 ccacattttc cgttgaggac atttcaagct acggtgttga agggctaatc gatgcaatag
    15421 aaaaattttc tcccgacaaa ggcgcgagat ttgaaactta cgcgcttatg agaattagag
    15481 gaaacattat cgacaaaatc cgttcacagg attttttgcc aagaagtgcc agaagaaaaa
    15541 tcaaagaagt aaaagaaacc caagaacagt taagacaaaa gctcggcaga atgcctacct
    15601 ctgctgaaat cggcgaagtt ttagggcttg aaaaagaaaa aatagaccag attttagccg
    15661 aagataccac gattacttca atatacgaca aaaagggctc atcggaagaa agccttgaaa
    15721 tcattgatac aatccaagat acccatagac ttaccccgca cgaagaaatg gaagaaaaag
    15781 atgttaaaaa agagcttgaa agagccctaa aaaaacttcc ggagcgcgag cgtatgatta
    15841 tggtacttta ctaccacgag aacatgacct taaaagaaat cggagaaa
//
LOCUS       DAOQAU010000039        15393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_4135_length_15393_cov_6.334985, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000039 DAOQAU010000000
VERSION     DAOQAU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 15393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15393
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4135_length_15393_cov_6.334985"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKI94_06375"
     CDS             <1..328
                     /locus_tag="PKI94_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26398.1"
                     /translation="SNIDLWATDGHVLFDRDTKEILNALKAPVTIGNHCWVGNSVKFT
                     KNANLPDNTVVGVGSVVTKTFTEEYTVIAGSPAKVVKTNICWARKTALAMQKELELNN
                     IGALKI"
     gene            450..2639
                     /locus_tag="PKI94_06380"
     CDS             450..2639
                     /locus_tag="PKI94_06380"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNW26399.1"
                     /translation="MKAQECFGKMKKMVETKEKLLNVKKTQVEIGGKIVEIETGKYAQ
                     TANASVTVRCGDTMLFVHATVSKEPRVGIDFFPLLIDYEERMSSIGRIPGGYNRSEGR
                     SSEKAILVSRLIDRPIRPLFPKGYRNDIQIVAQLFSYDQENQPDTLAMLGASFALMLS
                     GAPFEGPIGAVRVSKLDGKLIANPTHHQADKSDLDIVVSGTEDSVIMVEAGCKFVTED
                     DIMEAVEFAQAEIKKQCQAQIAFAKECGIVRQEFVNPHDTTELAEIIKKTAYDLVYDA
                     YHNFDRTYRQNKLDEAKKLVKEKIQALSEDHAINKMMEETGIDFVSEEFKSLEKKVMR
                     AMIIDEGVRADGRKPEEVRPIWCEVGVIPRAHGSAVFTRGQTQALSVATLAGPGMKQE
                     LDGVDPETEKRYMHKYIFPGFSVGEVKPMRGPGRREVGHGCLAERANIPALPTAEEFG
                     YVIRVTSDILESNGSTSMASTCGSSMALMNAGVPVKCMIGGVAMGMIAEQGKNPVVLT
                     DIQGIEDFLGDMDFKVTGNDEAITALQMDMKAKGISNDTLRKALAQAKEGRLHILSKM
                     REAIKAPGEMSPFAPRIFTMQIANEDIGAVIGPGGKQIRAIVDASGAEIDIQDSGVVT
                     ITSNDQEGADIAMNMIKNITFKPQEGMVLKGKVVRIIPIGAFVELGGGKDGMVHISQV
                     CNERIDAIEPHLNVGDVVVVKVIKIDDKGRVNLTIKGVTEQEKAQIS"
     gene            complement(2703..3035)
                     /locus_tag="PKI94_06385"
     CDS             complement(2703..3035)
                     /locus_tag="PKI94_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26400.1"
                     /translation="MREIKNDVMFGNIQQKKEVKTGKLNSVIPQVEGKDTDDLSVSSE
                     VLGRAQVNSPDALQKDVSFASAHPEAIEKADKFFDMTYKTLCESGDTDAYAKASLLTK
                     AYVDEFQR"
     gene            complement(3252..3968)
                     /locus_tag="PKI94_06390"
     CDS             complement(3252..3968)
                     /locus_tag="PKI94_06390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW26401.1"
                     /translation="MNIYYEKIKCEKADYEEAYWEIIKDPDGKTRNRLDEKDKFLANV
                     KKEVNFINALPPSKILDAGCGPGFLLSAIDKKHQKFGLEVSKFAADYAKQFATIYNAP
                     LEKAQIKENSFDVVISHHVIEHVDNPEVFLSEIKKILKPDGHLILATPDFKSICAKLF
                     KQNYRMLFDKTHTNLFSFNSLKKMLNDYDFDILEADFPYFETEYFSESNILKMLNFKS
                     GEISPACWGNFITFYCKNKK"
     gene            4112..4255
                     /locus_tag="PKI94_06395"
     CDS             4112..4255
                     /locus_tag="PKI94_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26402.1"
                     /translation="MYSPEFMRYLINYQTPEYNENIYKKTKNFKFKSGPRRWAEVLLL
                     NKK"
     gene            complement(4257..7238)
                     /locus_tag="PKI94_06400"
     CDS             complement(4257..7238)
                     /locus_tag="PKI94_06400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW26403.1"
                     /translation="MKTQTKPLDIDLSFKKNNVDELFFNLSENPDGFKNKDFRYTLSL
                     IYQIVEDEFQDVFLSPNSPVRNTDFYLINNKNNLSFFVTPTNNFEFYLKSKGSLFKFH
                     SDNLEGKQGFILPLDLVLDYFGGFAEIIDFSAVSETFVYLNKLTQFVLKLAEKMYFIP
                     TVKLNGNFFKIIYEPIISNSQLARIINRLEENMPETLFIKGEKPKNFVSEFVRNYLNY
                     IVYKFLSIKAYKFKNVKSGHYLIKDLEQKTSLKNVDIAENFSQWFDELYLGKYDVIPF
                     FNISKLEDELFELKVHIKNRKTNESVLIDDLYTKEEVFGANSSDIAKIVEKQLNYAIK
                     YMPELENLFEDEDNLSLKLNLGEVYKMITKTAYYLQKAQIEVILPKELVNVIVPRASI
                     NAKVKKAREGDLSDIFNNTAPSSISLEDILDFSYEVAIGDEKVSLKEFEKLTSQNEGL
                     IKYKDKYILVDKDESKKIFEQISKANHSKLSRLELIHASMSGQLENYDFDYDEAFANI
                     LKDFSKPLEVSIPKDLTGELRPYQKTGLKWLWTNISKGFGCCMADDMGLGKTIQVISL
                     ILKLKEENQLKRPVLVVCPTTLLGNWMKELQMFAPKLKAVIYHGSERQLELNNDIILT
                     TYAILRIDAEELRKQAWSMLVVDEAQNIKNPDTAQTLAVKSIKSDIKIAMTGTPVENR
                     LTELWSIFDFINKGYLGSLRDFQKSYAIPIERFKENTRASKLKMSVSPFVLRRLKTDK
                     NVITDLPEKLVLEDYCYLSKPQAVLYEKTLKEMMDKISGFSGINRRGNIFKLITALKQ
                     ICNHPYQFLKAGEMSKEFSGKMEKCISLVQNILENGEKTLIFTQYKEMGDILEQVLTE
                     ECSTKPLFFHGSLNVAQREKLIDEFQNNPETKVMILSLKAGGTGLNLTSATNVIHYDL
                     WWNPAVEDQATDRTYRIGQDKNVMVHRLVTLGTFEEKIDEMLKNKKELANLAVYEGEK
                     IITELSDDEIYEIFTLSGGNN"
     gene            complement(7447..8973)
                     /gene="gpmI"
                     /locus_tag="PKI94_06405"
     CDS             complement(7447..8973)
                     /gene="gpmI"
                     /locus_tag="PKI94_06405"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNW26404.1"
                     /translation="MNKRVLLCIMDGWGISENNPETNATILANPVNIDSFKKSEKYTQ
                     IHADGEWVGLPDGQMGNSEVGHLNLGAGRVVYQELTKINKSIREGDFFKNEKFLEAIN
                     HAKQNNSALHLFGLVSDGGVHSSMDHLAALIKLAADNGLKKIYVHGFLDGRDTPPQSA
                     CKYLQQVEDELKKYNLPSVASVMGRYWAMDRDNRWDRVEKAYNNLLFGEGEKAASAVE
                     AVQQSYAKGENDEFVKPTQIGGEESRIKDNDSIIFFNFRPDRAREITKAINFENFSGF
                     ERKDVRKNLHYTTMTQYDETFNFPVAFPPQKLNNLLGDVLEKNGIKQFRTAETEKYAH
                     VTFFFNGGIEEPNPNETRVLVPSPKVATYDLQPEMSAPEVCQKVLEAQDNPDYGFILV
                     NFANPDMVGHTGDIKAATKACKVVDECVGKIAQKAKEKGITMLLTADHGNSEEMFDPK
                     TQGPQTAHTTNEVPFFVINGDKNIELKKDGALCDVAPTVLKLLDIKKPEEMTGQSLIK
                     "
     gene            complement(9043..10683)
                     /locus_tag="PKI94_06410"
     CDS             complement(9043..10683)
                     /locus_tag="PKI94_06410"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNW26405.1"
                     /translation="MSTIEFFTDSEELKSITSAARATLTAPFFANNIKEIRDLKEAYL
                     LAKNSSGTIELTGMPVYKPEKLGLPSGTNQLLFNDGTVVGRCAAARKIVGEPGCDLAQ
                     LLPILREAVYCVRDRLMYKTQVVVGLEEDFMVKAHLLIPEGFENVMYSWMLNFQYITN
                     EYAKMYKNSRKINEGDIFVFSDPDWTHPDFPYGLTFFDPEHNCAAILGMRYFGEHKKG
                     TLTLAWGAAARNGYASCHGGMKRYNLKDKSFQTAVFGLSGSGKSTITHAKHNNKYDIT
                     VLHDDAFIINVDKKYSIALEPAYFDKTQDYPMGCEDNKYIITQQNNGAIQGKDGKIYS
                     ITEDIRNGNGRAVKSKLWSPNRVDRVDEPINAIFWLMKDSTIPPVIKLSGASLGSAMG
                     ATLATKRTSAERLAQGVDPDALVVEPYANPFRVYPLEMDYTRFKQLIEDGVDCYILNT
                     GDFMGKKVKPENTLGIIEAIVEGSAEFKKWGNFSDIQILKVEGFEANFDNAEYKDQFI
                     KRMKDRIEFVKSREVEKAGLDKLPADALEALEKVIAQV"
     gene            10892..12508
                     /gene="nadB"
                     /locus_tag="PKI94_06415"
     CDS             10892..12508
                     /gene="nadB"
                     /locus_tag="PKI94_06415"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNW26406.1"
                     /translation="MKYSKYSVAIIGSGIAGLYAALKISQKCDLPDGLLLITKSKLGE
                     SNSRYAQGGIVAVLKENKKDSPSLHISDTIKAGAGLSELGITKFISENSDEVIKDLIS
                     FGVEFDKNGNDNYYFTLEAAHSVNRILHSGGDATGRSIEKVLCQKVLENSNIEVYEQT
                     LAVELLVDSDSECKGFVAYNDLTKEYEAVYSSAVILASGGLGQLYKHTTNPTVATGDG
                     IALAYNAGAIIQDMEFVQFHPTALALDTGKNRFLISEAVRGEGAKLVDKDGHEFMQKY
                     HEQKELAPRDIVTRAIFNEMNKTNSDCVYLNSTHLSKKKLQKRFPNITRICEENGIDI
                     SKDFIPVAPAAHYSMGGVKTNVEARTSIRGLYAIGETASTGLHGANRLASNSLLECVV
                     CAYELADYLKETELNPPKLIDENLKNSIDKYSKDVDFEEVNVDLLKSKLQNLMWDYVG
                     ILRDEKSLTTANNKLHALKSEFKFPGKCSNKEEYEFKNMLTVAQLIIDSALKRTESRG
                     AHFRKDYPWAKENVEHNCIIKGEKGLVFVS"
     gene            12498..13328
                     /gene="nadC"
                     /locus_tag="PKI94_06420"
     CDS             12498..13328
                     /gene="nadC"
                     /locus_tag="PKI94_06420"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNW26407.1"
                     /translation="MFHDFLIEKHIKAALEEDIGFGDITTDFIAGEKDVLKGSLNTRE
                     NGVLCGCKVFEKVFKVLSDKVEIKFYFKDGDFIAAGDKIADIKGPARFILTGERTALN
                     YIQRMSGIATETKKYQDAIGNHPAKIADTRKTTPGFRMFEKYAVKTGGGALHRFNLSD
                     CAMIKDNHIKLAGSITKAVEKLRKNISHAHKIEVECDTLEQVKEALACNVDIIMLDNM
                     GVNEIREIVKLIDKRAIVEVSGGVNLDAVKEIAAAGVDIISSSAIVAKACALDLGLDI
                     "
     gene            complement(13325..14674)
                     /locus_tag="PKI94_06425"
     CDS             complement(13325..14674)
                     /locus_tag="PKI94_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNW26408.1"
                     /translation="MGEIGTILFNLFVIALLLFTNGFFVASEFAIVSVRKTRIAQLTK
                     EGNFNAKIALEALKDLDRFIAAVQLGITISSIGLGWVGEATLVRIIEPLFDFLPQNYQ
                     LVAAHSISVAIAFSLITVMHVVIGELMPKSIALQYPEKVSLIIAVPMKVITKFFSPFI
                     FMLNGFGNLLLKIFKIPTSPASHLAHSIEELNMLINASYNEGVLNETEREMLQNVFKF
                     SDLTAKQVMVPRTDMACISSDITFDELNTLTEEMQYTRYPVYEENLDHISGILHVKDL
                     YGISLRKEEFSLEKLLRPVFLVPETITMDNLLREFKKRKSQMAIVIDEFGGTSGLITL
                     EDVLEEIFGDVQDEFDEEEADIKEISPNTYIANAMMRIDEIAEFFEIELVDEDVDTIG
                     GLVLKELGRIAQINDCVEIKDLEFSVQEIDGARITKLIIIKKNKENEQKENVEEENS"
     gene            14934..15182
                     /locus_tag="PKI94_06430"
     CDS             14934..15182
                     /locus_tag="PKI94_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNW26409.1"
                     /translation="MRLDKFLKVSRLIKRRTVANEISDKGRIFVNGNQAKPSKMLKEG
                     DIIEIKNPPVKVRVLKIPQGNVSVQEAPSLYEVMLYQT"
BASE COUNT         4670 a         2740 c         3026 g         4957 t
ORIGIN      
        1 atcaaatata gatttgtggg ctacagacgg gcatgttctt tttgatagag atacaaaaga
       61 aatactaaat gcgttaaaag cccctgttac tattggaaac cactgttggg tgggcaattc
      121 cgttaagttt accaaaaatg ctaacctgcc cgataacacg gttgtcggag taggttctgt
      181 ggttacaaaa acttttactg aagaatatac tgtgattgcc ggtagccctg ctaaagtcgt
      241 aaaaaccaat atctgttggg cacgaaaaac agctcttgct atgcagaaag aattagaatt
      301 aaataacatt ggtgcattaa agatttaagc cttttgtttt gaaatttata tttttatttt
      361 taatttgttt aaaaaaatga agcaatgttt ctacttttgt tatgtttgaa gtataatctt
      421 attgtagtaa ataagtgtat aacttagaaa tgaaagcaca agagtgcttc ggaaagatga
      481 agaaaatggt agaaacgaaa gaaaaattac ttaatgtgaa aaaaacacaa gtcgaaatcg
      541 gcggaaaaat tgtagaaatt gaaacaggaa agtatgctca aacagctaac gcttctgtaa
      601 cagtaagatg cggagacaca atgctttttg tccacgcaac agtaagcaaa gaaccaagag
      661 tcggaataga cttttttcct ttgctgattg attacgaaga aagaatgtct tcaataggaa
      721 gaatcccggg cggatacaac cgttctgaag ggcgttcgag tgaaaaagcc attttggttt
      781 caagattaat agacagacca atcaggcctt tgtttccaaa aggttacaga aacgatattc
      841 aaattgtggc tcaattattc agctatgatc aggaaaatca acccgataca ttggcaatgt
      901 tgggtgcatc atttgcgctt atgttgtcag gagccccttt tgaagggcct atcggcgcgg
      961 taagagtgtc aaaacttgac ggaaaactta ttgcaaatcc tacccaccat caggcagaca
     1021 aatcagattt agatattgtt gtttcaggaa cagaagattc tgtaattatg gttgaagcag
     1081 gctgcaaatt cgtaacagaa gacgacatta tggaagcagt tgaatttgca caagctgaaa
     1141 ttaaaaaaca atgtcaggct caaattgctt ttgcaaaaga atgcggtatt gtaagacaag
     1201 aatttgtaaa tcctcacgat acaacagagt tggctgaaat tatcaaaaag acagcttacg
     1261 atttggttta tgatgcttac cacaattttg acagaaccta tagacaaaat aaattggatg
     1321 aagctaaaaa acttgttaaa gaaaaaattc aggctttgtc cgaagatcac gcaataaaca
     1381 aaatgatgga agaaacagga attgactttg tatcagaaga atttaagtct ttagagaaaa
     1441 aggttatgcg tgcaatgatt atcgatgaag gcgtcagggc tgacggcaga aaacctgaag
     1501 aagtccgtcc aatctggtgc gaagtcggag ttattccaag agctcacggc tcggcagttt
     1561 tcaccagagg tcaaacccag gccctttcgg ttgctacatt ggcaggtcct ggcatgaagc
     1621 aagaacttga cggtgttgat cctgaaacag aaaaaagata tatgcacaaa tacatattcc
     1681 ccggattttc agtaggcgaa gttaaaccta tgagagggcc gggcagaaga gaagtcggac
     1741 acggatgctt ggcagaacgg gctaatatcc ctgcgcttcc tacagctgag gaattcggat
     1801 atgttatcag agttacttcg gacatacttg aatcaaacgg ttcaacttct atggcatcaa
     1861 cttgcggaag ttcaatggcg ttaatgaacg cgggtgttcc cgttaaatgt atgattggcg
     1921 gggttgcaat gggtatgatt gcagaacaag gcaaaaatcc tgtagtgctt accgatattc
     1981 aaggtatcga agacttttta ggcgatatgg actttaaagt tacaggtaat gatgaggcaa
     2041 taactgcact tcaaatggat atgaaagcaa aaggtatttc aaatgatact ttaagaaaag
     2101 cacttgctca ggcaaaagaa ggcagattgc atattttaag taaaatgaga gaagcaatca
     2161 aggctccggg tgaaatgtca ccgtttgcac cgagaatatt cacaatgcaa atcgctaatg
     2221 aagatatcgg tgctgtaatc ggaccgggcg gaaaacaaat cagagcaatc gttgatgctt
     2281 caggtgctga aattgatatt caagacagcg gtgttgttac gattacctct aatgatcagg
     2341 aaggtgcaga tattgcaatg aatatgatta aaaatattac cttcaagccg caagaaggta
     2401 tggtattaaa aggtaaagtc gtaagaataa tcccgattgg cgcatttgtt gaactcggtg
     2461 gcggaaaaga cggaatggta cacatttctc aggtctgcaa cgaaagaata gatgctattg
     2521 aaccgcacct taacgttggt gatgtcgttg tcgtcaaagt tattaaaata gatgacaaag
     2581 gcagagttaa ccttacgatt aaaggcgtaa ccgagcagga aaaagctcag attagttaat
     2641 cataaataat aaaaatgggg tgggattttg aaaaaatccc accccatttt tattgtaaaa
     2701 atttatcttt gaaattcatc aacataagct ttagtcagta agcttgcttt tgcgtaagca
     2761 tcggtgtcac cgctttcaca aagcgtttta tatgtcatgt caaagaattt atctgctttt
     2821 tcaatggctt caggatgtgc tgaggcaaaa gaaacgtctt tttgcaatgc atcagggctg
     2881 tttacttgtg ctcttcccaa aacttcggat gatacagaca aatcgtctgt atctttacct
     2941 tcaacttgag gtattacaga attaagtttg ccggttttaa cttctttttt ttgttggatg
     3001 ttaccgaaca tcacatcatt tttgatttct ctcataattt tatatccttt attgtgtttt
     3061 tccaaatctc tcttgtacat gcattaaaac gcaaacagat ttttttttgc tagtttattt
     3121 agaaattata taaatttttt tctaataact ttatttgaaa tgtcaataat ccaattatat
     3181 caacaaaaac tgcaaaatca aaagttatat aatttgctta acaaaactta atgttcgtgg
     3241 atttgcagga attatttttt atttttgcag taaaaagtta tgaaattgcc ccagcatgcc
     3301 ggagaaattt cgccgctttt aaaatttagc attttaagga tattgctttc actaaagtat
     3361 tccgtttcaa agtagggaaa atccgcttcc agtatatcaa aatcgtaatc gttaagcatt
     3421 ttttttaaag aattaaagct gaataaattt gtatgggttt tgtcaaaaag catgcggtag
     3481 ttttgtttaa agagttttgc gcagatgctt ttaaaatcag gtgttgctag gattaaatgt
     3541 ccgtcgggtt ttagtatttt tttaatttcc gacaaaaaaa cttcggggtt atcaacatgt
     3601 tcgattacgt ggtgtgagat aacaacatca aaggaatttt ccttaatctg cgctttttca
     3661 aggggggcat tgtagattgt tgcaaactgt tttgcgtaat ctgctgcgaa ttttgaaact
     3721 tcaagcccga atttttggtg ttttttgtct atcgcgctca gcaaaaatcc cggcccgcag
     3781 cccgcatcaa gaatttttga aggaggcagg gcattaataa aattcacttc tttttttaca
     3841 ttggcaagga atttatcttt ttcatccaaa cggtttcttg ttttgccgtc ggggtctttg
     3901 attatttccc agtaagcctc ttcgtagtcg gctttttcgc acttgatttt ttcataatat
     3961 atattcataa agataatata tcacgttttt tataaaacta aaatctatga atttatgtaa
     4021 aaaagtttat ttcaaaggca atttttttgt agaatatttg tttatataca tgtgtggaag
     4081 atttggaatt tttccaaaaa ggagtcggta gatgtacagc cctgaattta tgagatattt
     4141 gattaattat caaacccctg aatataatga aaatatttat aaaaagacaa agaatttcaa
     4201 attcaaatcc ggtccgcgca gatgggcaga agtcttgctt ttgaacaaaa agtagtttaa
     4261 ttatttccgc ctgagagtgt aaatatttcg taaatttcgt catcggaaag ctctgtgatg
     4321 attttttcgc cttcgtagac ggcaaggttt gcaagttctt ttttgttttt gagcatttcg
     4381 tcgatttttt cttcaaaagt gcccagtgtt acaagcctgt gcaccattac gtttttgtcc
     4441 tgtcctatgc ggtaagttct gtctgttgcc tgatcttcga cggcaggatt ccaccacagg
     4501 tcatagtgga ttacgtttgt ggcacttgta aggttcaagc ccgttccgcc cgcttttaag
     4561 gacaaaatca taacttttgt ttcggggtta ttttggaatt cgtcaataag tttttcgcgc
     4621 tgcgcaacat tcaatgagcc atggaaaaac agcggttttg tcgaacattc ttctgtcaga
     4681 acctgttcta aaatatcgcc catttccttg tattgggtga agataagggt tttttctccg
     4741 ttttccagaa tgttttggac aagtgaaata catttttcca tttttccgga aaactctttg
     4801 ctcatttcgc cggctttaag aaactggtag gggtggttgc aaatctgttt gagcgcggta
     4861 attaatttga aaatatttcc gcgtctgtta attccgctga agccgcttat tttatccatc
     4921 atctctttta aggttttttc ataaagcacc gcctgcggct tagaaagata gcagtaatct
     4981 tccagcacaa gtttttcggg aaggtctgtg ataacatttt tgtcggtttt aagccttctt
     5041 aagacaaaag gcgaaacaga cattttaagc tttgatgcgc gggtattttc tttaaaccgt
     5101 tcaatcggga ttgcataact cttttgaaaa tccctcaaag agcccaaata acctttgttt
     5161 atgaagtcaa aaatactcca aagctctgta agcctgtttt ctacaggagt acccgtcatt
     5221 gcgattttaa tgtcggattt aattgattta accgcaagag tctgcgccgt gtcggggttt
     5281 ttgatgttct gcgcttcatc caccacaagc attgaccatg cctgttttct aagttcttcg
     5341 gcatcaattc tcaaaatcgc gtatgtggtt aatattatgt cattatttag ttctaattgt
     5401 ctttcgctgc cgtgataaat taccgctttt aatttcgggg caaacatttg aagttctttc
     5461 atccagttac ccagcagagt tgttgggcaa acaaccagaa caggtctttt tagctgattt
     5521 tcttctttga gtttaagaat aaggcttatc acctgaattg ttttaccaag tcccatatca
     5581 tctgccatgc agcagccgaa cccttttgaa atatttgtcc aaagccattt taaacctgtt
     5641 ttttggtaag gccttagctc tccggttaag tctttcggga ttgaaacttc cagaggtttt
     5701 gaaaaatctt tcaggatatt tgcaaaggct tcgtcatagt caaaatcgta gttttcaagc
     5761 tgacctgaca tggaagcgtg gattaattca agccgcgaaa gctttgagtg atttgctttg
     5821 gagatttgtt caaaaatctt tttgctttca tccttgtcaa caagaatata tttgtctttg
     5881 tattttataa gtccttcatt ttgcgaagtt aatttttcaa attctttcag cgagactttt
     5941 tcatctccga ttgcaacttc gtaagaaaaa tccaaaatgt cttcaagtga tatactgctt
     6001 ggtgctgtgt tgttgaaaat atctgaaaga tcgccttctc ttgctttttt gactttggcg
     6061 ttaatagaag cacgcggaac aatgacattt acaagttctt ttggcaaaat gacttcaatt
     6121 tgggcttttt gaaggtaata agctgttttg gtaatcattt tatagacttc gccaaggttg
     6181 agtttaagtg ataaattgtc ttcgtcttca aaaagatttt caagctcagg catgtatttt
     6241 atggcataat taagctgttt ttcgacaatt ttggcaatat ctgatgaatt tgcaccaaaa
     6301 acttcttctt ttgtataaag atcgtctatc aatacacttt cattagtttt tcggtttttg
     6361 atgtgaacct taagttcaaa aagttcgtct tcaagttttg aaatgttgaa aaacggaatt
     6421 acatcgtatt tgccaagata aagttcgtca aaccactgcg agaagttctc cgcaatatca
     6481 acgtttttga gcgaagtttt ctgctccaaa tccttaatta aatagtgtcc cgatttgaca
     6541 tttttgaact tgtaagcctt tattgaaagg aatttataga ctatataatt cagataattt
     6601 ctgacaaatt cagaaacgaa attttttggt ttttcgcctt ttataaaaag agtttcaggc
     6661 atattttctt caagtcggtt tattattctt gcaagctgac tgtttgaaat tatcggctca
     6721 tagattattt tgaaaaaatt tccgtttaac tttacggtag gaataaaata cattttttca
     6781 gcgagtttca aaacaaattg agtcagtttg ttaaggtaaa caaaagtttc cgaaactgct
     6841 gaaaaatcaa ttatttctgc aaatccgccg aaatagtcca aaaccaaatc aagcggcaaa
     6901 ataaatcctt gtttcccctc taaattatcg gagtgaaatt taaataaaga gccttttgac
     6961 tttaaataaa attcaaaatt atttgtcggg gtgacaaaaa acgatagatt gtttttattg
     7021 tttatcagat aaaaatcggt gtttcttacc ggtgaattag ggctgaggaa aacatcttga
     7081 aactcatctt ccacaatttg ataaataaga ctaagcgtgt atcggaaatc cttgttttta
     7141 aatccatcgg gattttcgct caaattaaaa aacaattcat caacattgtt tttcttgaat
     7201 gataagtcta tgtctaaagg ttttgtttgt gttttcatat tctcccttta tacccctcgc
     7261 cccttgaggg agagggttga atttcaaaac gagtttacga gttttagaaa ttcgggtgag
     7321 gggttatttc gtccctcttt ttataattta ctttttggat tgccgcgtcc tcacttcatt
     7381 cgtcctcgca atgacgtgag cggcgaagca ttaagccaaa tcatttatcc catcgcaatg
     7441 acgtatttat ttaataagtg attgccctgt catttcttcg ggttttttga tgtctaacaa
     7501 ttttaacaca gtcggtgcga catcgcaaag agccccgtct tttttaagtt caatattttt
     7561 atctccgtta atcacaaaga aaggaacttc atttgttgtg tgtgctgttt gagggccttg
     7621 tgttttaggg tcaaacattt cttctgaatt tccgtgatct gcggtcaaaa gcattgtaat
     7681 tcctttttct tttgcttttt gtgcaatttt gccgacacat tcatccacga ctttgcaagc
     7741 ttttgtcgct gcttttatgt ctcctgtgtg tcctaccata tcagggtttg caaagttcac
     7801 cagaataaat ccgtaatcag gattgtcttg ggcttcaagc actttttgac aaacttcagg
     7861 tgcgctcatt tcaggttgca aatcataggt cgcaactttt ggtgaaggaa ctaaaaccct
     7921 tgtttcattg gggttaggct cttctatgcc cccgttaaag aagaaggtga catgagcgta
     7981 tttttctgtt tccgctgttc tgaactgttt tattccgttt ttttcgagca catcgccgag
     8041 cagattattt aatttttgag ggggaaacgc cacaggaaaa ttaaaagttt catcgtattg
     8101 ggtcatggtg gtgtagtgaa ggttttttct gacatctttt ctttcaaatc ccgagaagtt
     8161 ttcaaagtta atcgcttttg ttatttctct tgctctgtcg ggtctgaaat taaagaaaat
     8221 tatagaatca ttatctttta ttcttgattc ttcgccacct atttgggtcg gttttacaaa
     8281 ttcatcgttt tcgccttttg cgtaagattg ttgcactgct tcaaccgcag aagctgcttt
     8341 ttcgccttct ccgaataaaa gattattata agctttttcg actctatccc agcggttatc
     8401 tctgtccatt gcccaatagc ggcccatgac agaggctaca gagggcaggt tgtatttttt
     8461 taattcatct tcaacctgtt gcaggtattt gcaagcactt tgcggtggag tgtcgcgccc
     8521 gtctaaaaat ccgtgtacat agattttttt tagtccgtta tctgctgcaa gcttaattaa
     8581 agcagccaaa tgatccattg aggagtgaac gcctccgtca gaaaccaacc cgaacaggtg
     8641 aagtgcagaa ttgttttgtt tggcatgatt tattgcttca agaaattttt cgtttttgaa
     8701 aaagtcacct tcacgaatac ttttatttat ctttgttaat tcctgataca caactcttcc
     8761 tgcgcctaag ttaaggtgtc cgacttcgga gttgcccatt tgtccatcgg gaagccccac
     8821 ccattcaccg tcagcgtgga tttgtgtgta tttttcggat tttttaaaac tatctatgtt
     8881 gacggggttt gccaaaatag tggcatttgt ttcgggatta ttttcgctta ttccccagcc
     8941 gtccattatg cacaataaaa ctctcttatt catatctttt ccttgccaaa tgtaatcatt
     9001 aatacatttt agcaggaaaa cagaacaact tcaaggtaaa aatcaaactt gcgcaattac
     9061 tttttcaagt gcttcaagtg catccgcagg aagtttgtcc agtcctgctt tttcgacttc
     9121 gcgcgatttt acaaattcaa ttctgtcttt catccgtttt ataaattggt ctttgtactc
     9181 ggcattgtca aaatttgctt caaagccttc gactttaagg atttgaatat cagagaagtt
     9241 gccccatttc ttaaattcgg cactgccttc gacaatcgcc tcgattattc ccaaagtatt
     9301 ttcaggctta acttttttgc ccataaaatc gccggtgttt aggatatagc agtcaacgcc
     9361 gtcttctatt aactgtttaa atcttgtgta atccatttct aaaggataaa cccggaaggg
     9421 attagcgtaa ggttcaacca caagcgcatc gggatcaacg ccttgggcta atctttcggc
     9481 tgaagttctt tttgtggcaa gagttgcacc catagctgag cccaaagaag cacctgaaag
     9541 tttaatcaca ggcggaattg tagaatcctt cattagccaa aaaattgcat taataggttc
     9601 atccaccctg tcaacgcgat ttggtgacca gagttttgat ttaaccgccc tgccgttgcc
     9661 gtttctgatg tcttctgtga ttgaataaat ttttccgtct ttgccctgaa tcgccccgtt
     9721 attttgctgg gtaattatgt atttgttgtc ttcgcagccc ataggataat cctgcgtttt
     9781 gtcaaaatat gcaggctcta acgcaatgga atattttttg tcaacattta ttatgaaagc
     9841 gtcatcatgc aaaaccgtaa tatcgtattt attattatgt ttggcatgcg taatggtgga
     9901 tttgcccgag cccgaaagcc caaaaactgc agtttgaaaa gatttatctt ttaagttgta
     9961 gcgtttcatt ccgccatggc aagaagcgta gccgtttctt gcggcagctc cccaggcaag
    10021 tgtaagagtg ccttttttgt gttctccgaa atatctcatc cctaaaattg cagcacagtt
    10081 atgttcagga tcaaagaagg tcaacccgta aggaaaatcg gggtgagtcc agtcggggtc
    10141 ggagaaaaca aaaatatcgc cttcatttat ttttcttgaa tttttgtaca tttttgcata
    10201 ttcgtttgta atgtactgga aattgagcat ccatgaatac ataacatttt caaaaccttc
    10261 ggggataagc aggtgtgctt ttaccataaa atcttcttcc agtcccacta caacttgggt
    10321 tttatacatt aacctatcgc gcacacaata gaccgcttct cttaaaatag gtaaaagctg
    10381 agccaaatca caacccggct cgccgacgat ttttcttgcg gcggcgcatc ttcccacaac
    10441 cgttccatca ttgaacaaaa gctggttagt gcctgaggga agccccagtt tttcgggttt
    10501 ataaacaggc attcctgtaa gttctattgt gcccgaagaa tttttggcaa gaaggtaggc
    10561 ttcttttaaa tctcttattt ctttaatatt gttggcaaaa aagggagcgg ttaaagttgc
    10621 cctggcagcg gatgttattg attttaattc ttccgaatca gtaaaaaatt ctatcgtgga
    10681 cattgaattc tccttaaagt tcacgtcatg ctgaattcat ttcagcatcg caaggtcgtt
    10741 tgccaaaata ttaagtcatg agatcttgaa tcaagttcag gatgacgtat aataatttta
    10801 cttcaaacaa atttctttgt aaaaaattag tcttttgcgg tagaaaaaaa tttttgttta
    10861 ttttaaaatg tcctggatag gatatgttat tatgaagtat tcaaaatatt cagttgcaat
    10921 aataggaagc ggaatcgcag gactctatgc ggccttgaag atttcccaaa aatgcgattt
    10981 gccagacggc ttacttttga ttacaaaatc aaaactggga gaaagcaatt cgcgctacgc
    11041 tcagggcgga attgtagcgg tcttgaaaga aaacaaaaaa gattctccgt ctcttcatat
    11101 cagcgacaca attaaggcag gtgcgggatt aagcgagctt ggcataacaa aatttatctc
    11161 cgaaaattcc gatgaagtta taaaagactt aatttcattc ggtgttgaat ttgataaaaa
    11221 cggaaacgat aattactatt ttaccctaga agccgcacac agcgtaaacc gtattcttca
    11281 ctcaggcggt gatgcaacag gaagaagcat tgaaaaagtg ctttgtcaaa aagtcttgga
    11341 aaattccaat attgaagtct atgagcaaac tttagccgta gaacttttgg tggattccga
    11401 cagcgagtgt aaaggttttg tcgcatataa cgatttaaca aaagaatacg aagcagtcta
    11461 ttcatctgct gtgattcttg cctccggtgg gctcggtcag ctttataagc atacgacaaa
    11521 cccgacagtt gcaacaggcg acggaatagc acttgcctat aacgcagggg caataattca
    11581 ggatatggaa tttgtgcagt ttcacccgac ggcacttgcg ctcgatacag gcaaaaatag
    11641 gtttttgata tccgaagccg taagaggcga aggcgcaaaa ctcgtggata aagacgggca
    11701 tgagtttatg caaaaatacc atgaacaaaa ggagcttgcg ccaagagata tcgttacaag
    11761 ggcaatattt aacgaaatga ataaaactaa ttcggattgc gtttatttga attcaactca
    11821 tttgtcaaag aaaaaacttc aaaaacgatt ccctaatatc acaagaattt gtgaagaaaa
    11881 cggaatagat atttcaaaag atttcatccc tgtagcaccc gccgcacact attcaatggg
    11941 cggagttaaa acaaacgtgg aggcaagaac ttctataaga gggctttatg caatcggcga
    12001 aaccgcttca acaggattac acggtgcaaa ccgccttgca agcaattcgc ttcttgaatg
    12061 tgtcgtttgc gcttatgagc ttgcagatta cctgaaggaa acagagttaa atccgccaaa
    12121 gctgatagat gaaaatttga aaaattcaat agataaatat tctaaagatg ttgattttga
    12181 agaagtaaat gtggatttgc ttaaatcaaa acttcaaaac ctgatgtggg attatgtcgg
    12241 aattcttcgc gacgaaaaat ccttgacaac ggcaaacaat aagcttcatg ctttaaaatc
    12301 cgaatttaaa tttcccggca agtgttcaaa caaagaagag tatgaattta aaaatatgct
    12361 taccgtcgct caattaataa ttgattcggc acttaaaaga acagaatcaa gaggtgctca
    12421 tttcagaaaa gactaccctt gggcaaaaga aaatgtcgaa cacaactgta ttataaaagg
    12481 agaaaagggg cttgtttttg tttcatgatt ttttaatcga aaagcacatt aaggcggcac
    12541 ttgaagagga tatcggcttt ggcgatatta cgacggattt tatcgcggga gaaaaagatg
    12601 ttttaaaagg ctctcttaat acaagagaaa acggtgttct ttgcggctgc aaggtttttg
    12661 aaaaagtttt taaggtttta tctgataaag tcgagataaa attttatttt aaagacggcg
    12721 attttatcgc cgcaggcgat aaaatcgccg atataaaagg tcctgcaagg tttattttaa
    12781 caggcgaaag aacggcactg aattacattc aaagaatgag cgggatagcc acagagacaa
    12841 aaaaatatca agatgcaatc gggaaccatc ctgcaaaaat cgccgataca agaaaaacaa
    12901 ccccgggctt cagaatgttt gaaaaatatg cggtaaaaac aggcggaggg gcactgcatc
    12961 ggtttaattt atctgattgc gcgatgatta aggacaatca catcaagctt gcgggctcta
    13021 ttacaaaagc ggtggaaaaa ttaagaaaaa acatttctca cgcacataaa attgaagtcg
    13081 agtgcgacac tttagagcaa gtcaaagaag ctttagcttg taatgttgac ataataatgt
    13141 tagataatat gggggtaaat gagataagag aaatagtcaa gctcatcgat aaaagggcaa
    13201 ttgttgaagt aagcgggggt gtaaatttag acgcggttaa agaaattgct gcagcgggag
    13261 ttgacataat ttcatcaagc gcaattgttg caaaggcttg cgcacttgat ttggggcttg
    13321 atatttaaga attttcttct tcgacgtttt ctttttgttc gttttcttta ttttttttga
    13381 tgataataag ttttgttatt ctagcaccgt caatttcttg aacgctaaat tctaaatctt
    13441 taatttcgac acaatcgtta atttgtgcta ttctgcctag ttcttttaaa accaagccgc
    13501 cgattgtatc aacatcctca tccacaagtt cgatttcaaa aaattctgct atttcatcta
    13561 ttctcatcat cgcatttgcg atgtaggtat tgggtgaaat ttcttttata tcggcttctt
    13621 cttcgtcaaa ttcatcttga acgtcgccga aaatttcttc aaggacatct tctaaggtga
    13681 taagtcccga tgttccgcca aattcatcta taacaatagc catttggctt ttgcgttttt
    13741 taaattcacg aagaagatta tccatggtaa ttgtttcagg aacaagaaaa acaggtctta
    13801 aaagtttttc cagtgaaaat tcttcttttc ttagagaaat tccgtacaaa tctttaacgt
    13861 gtaaaattcc tgaaatatgg tcaagatttt cttcataaac aggatatctt gtatattgca
    13921 tttcttctgt gagtgtattt aattcgtcaa aagtaatatc ggaggaaata caagccatat
    13981 cagtccttgg caccataacc tgttttgcgg ttaagtcgga gaatttgaaa acattctgga
    14041 gcatttcacg ttcggtttcg tttaaaacgc cctcattata gcttgcgttt attagcatat
    14101 ttaactcttc aatagaatgg gcaagatgtg aagccggtga ggtcggaatt ttgaaaatct
    14161 ttaacaataa atttccaaaa ccgtttaaca taaaaataaa cggactgaaa aattttgtaa
    14221 taactttcat cggaacggca attattaaag atactttttc gggatattga agtgcgattg
    14281 actttggcat caattcacct attacgacgt gcataacggt aattaaagaa aaagcaattg
    14341 caaccgaaat tgaatgagcg gctacaagct gataattttg aggtaagaaa tcaaataaag
    14401 gctcaattat tctgaccaaa gtagcctcgc ctacccaacc cagaccgata ctggagattg
    14461 taattcctaa ctgtactgct gcaataaatc tgtctaaatc cttaagtgct tccagcgcaa
    14521 tttttgcgtt aaaattgcct tcttttgtaa gttgtgcgat tcttgttttt ctgacgctta
    14581 ctattgcaaa ttctgacgca acaaaaaacc cgtttgtaaa aagcagaagt gctattacaa
    14641 acaaattaaa caatatcgtg cctatttcgc ccattaatta tccttagtct gaatttttta
    14701 aaattatatt caaatccatt ataatattgt aaagcaaaat ttgcaacttt tatgttaaaa
    14761 tcatataatt ggtcagtgag tgattgatag taattgtttc ttatcccctc gccccttgag
    14821 ggagagggta gaattttaaa atgaggtacg aattttaaaa attcgggtga ggggtattac
    14881 gaagaaaata gtgatgttta aatatctcga cataaaaata ggatgcttga aaattgagac
    14941 tagataaatt tttaaaagtg tcaagattaa taaagagaag aaccgtggca aacgaaattt
    15001 ctgacaaggg gcggattttt gttaacggta atcaggcaaa accttctaag atgcttaaag
    15061 aaggcgacat tatagaaatt aaaaatccgc cggtaaaagt aagagtctta aaaattccgc
    15121 agggaaatgt cagcgtacag gaagcaccgt cactttatga ggttatgctt tatcaaacgt
    15181 aattgttatc ctttttgtcg ttgcaagggg gtaaatattt tccaacccat gtccacctcc
    15241 tacgtcgttg cgaggaaacg gtaagcctgt cctgaacttg tttcaggaac aaagcaatct
    15301 aaaaactcag gattaataaa ttgacttttc tctggggggg ggggggtata attgttaggg
    15361 tttaaaatta aaatattcaa ggttttttat gaa
//
LOCUS       DAOQAU010000040        15326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_4162_length_15326_cov_6.605265, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000040 DAOQAU010000000
VERSION     DAOQAU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15326
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_15326_cov_6.605265"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PKI94_06435"
     CDS             <1..1254
                     /locus_tag="PKI94_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNW26410.1"
                     /translation="GNMGLTIAEKIISRHANKEVEAGELVIANVDVCAVQDGTGPLTV
                     QEFKKIGAKKLNNPQRTILFIDHASPSPRKELSNSHNVLREFAKEYGAVLSDVGAGVC
                     HQRLIEDYVNPGEILIGADSHTCTSGALGAFATGMGSTDVAVGMALGKTWLKVPQTFK
                     IQVDGEFRQGVCSKDLMLYLIGLIGADGATYKALEFCGSTIDNMYMAERFTLANMAVE
                     AGAKAGLFATDEKTKEYLQSRGRGDKFVEIKPDVDAHYERVIKIDASIVGHMVSCPHT
                     VDNVKPASELRDVKVNQVFVGTCTNGRIEDLRIAANILKGKKIHPEVRMLVSPASKEV
                     FKKALREGLIDIFVDANATILAPGCGPCVGVHAGILADNEVCLATQNRNFQGRMGNTK
                     SFIYLSSPYVATCSAIKGFITSSSF"
     gene            complement(1235..1993)
                     /locus_tag="PKI94_06440"
     CDS             complement(1235..1993)
                     /locus_tag="PKI94_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNW26411.1"
                     /translation="MNDIFSRNELYWGKEGQKKLSESHIIVFGLGGVGGYCVEMLARA
                     GIGELTLVDFDKVSVSNINRQIVALNSTLGQEKTTLFEKRLKDINPEINVHLINDFYT
                     ESLNSILIGKNIDFIADAIDTIRSKVSLLEFAYTEKFPVISSFGAGNRVNPEGLYICD
                     ISKIKNKKTPFISNIIHQLKLRNINEGIMVVTSDEKPMCQNKILNTETVETQRGEKIE
                     YTKITPASTPFVASAAGIFMASHIVQELTKKKKK"
     gene            complement(1993..2217)
                     /gene="secG"
                     /locus_tag="PKI94_06445"
     CDS             complement(1993..2217)
                     /gene="secG"
                     /locus_tag="PKI94_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNW26412.1"
                     /translation="MLTFTWIVQIFSAILLIVLILLHSPKGDGIAGIGGASQLFSSQK
                     SAEAGLNKLTGYVAAVFLLCTFLLGFGIIK"
     gene            complement(2233..2919)
                     /locus_tag="PKI94_06450"
     CDS             complement(2233..2919)
                     /locus_tag="PKI94_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNW26413.1"
                     /translation="MFNETKTHEITVDVVILTIKDNKLMVLLVKRTNEPFKGKWAIPG
                     GFIRLSENLDEAALRILKEKTNVENIYIEQLYTFGDPLRRTDARVITCAYFALIREED
                     VHIISSPDVSWHRVYELPPLAFDHKEIIEYSLKRTRERLEMCPVAYQLLNEKFTLTEM
                     QKAYELIMNKKLDKRNFRKKVLSTDGLTELNEFSKSKSKRPARLYTFEKISLNSKRAH
                     FISKPKTKKD"
     gene            complement(3034..3660)
                     /locus_tag="PKI94_06455"
     CDS             complement(3034..3660)
                     /locus_tag="PKI94_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNW26414.1"
                     /translation="MIRKLVLYTITIVLLLCPKVNASQHSVSTVKETIVKHSLELGID
                     PALALSIAKSESGFRHEARSRYGAVGVFQLMPSTAKRLGVNPYYLSDNIKGGLMYYKR
                     LYNMFGSTELALAAYNAGPGAVKRYKKVPPIGETRSFVSKIMKEYNRQKINPDPAILK
                     VKNMKQDIKQVELKQLEEKKPEVKAVEVKEIETTNVQTQEPLPSQGVV"
     gene            3883..4569
                     /locus_tag="PKI94_06460"
     CDS             3883..4569
                     /locus_tag="PKI94_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNW26415.1"
                     /translation="MKNKFGFTLAEVMVVLVTLGVLAAIAIPAVVHVRPNNKKVMLKK
                     AYSTLEKAVQELINNDTYYPYEADDGNTGAQYLWGLSTDITPIRVWKGFNYVYNKEAL
                     GIPIGDNKFCYLLANSMNTVGAIDCAIFGTHTSMSDANFKTADGITWTMLTNQAADAQ
                     FPLNQNDYFNISVDVNGTKDPNCGSGVFTTQSCASSGPDQFDFSVRYDGKIKVSNAAG
                     IIITDPATNH"
     gene            4603..5409
                     /locus_tag="PKI94_06465"
     CDS             4603..5409
                     /locus_tag="PKI94_06465"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNW26416.1"
                     /translation="MSEKKQLAKIGVFGGSGFYKFLDNIEEIRVETPYGMPSDNIFVG
                     KLGNKKVAFMPRHGRNHSIMPHLINYRANVWAMKSIGVERVISPCAAGSLQKHVVPGD
                     FVICDQFVDWTDGRKSTTIEGPIVSHPSPADLYCPEMRNLVIDTAKKLGVNVHSTGTV
                     VVINGPRFSTKSESKFFTNQGWEVINMTAFPENYLVREMDMCPLNISLITDYDAGLVG
                     DVPPVSHHAVMEVFNSNLDRLKKVLFTMIEQIPDERENCECSKTSVNSRL"
     gene            5428..5694
                     /locus_tag="PKI94_06470"
     CDS             5428..5694
                     /locus_tag="PKI94_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNW26417.1"
                     /translation="MYTVKIVDNIFYFFYLIIILRIFLTWIPNIDWEQQPIKAIREVT
                     DMYLDIFRRFIPPISGLDFSPIIALIFLQIIQIVVVNIVSSILG"
     gene            complement(5686..6774)
                     /gene="hemW"
                     /locus_tag="PKI94_06475"
     CDS             complement(5686..6774)
                     /gene="hemW"
                     /locus_tag="PKI94_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNW26418.1"
                     /translation="MTKNAYIHIPFCRSKCNYCSFVSFDKIDLKKAYLEALMYEIKTI
                     YKGEFLKTLYFGGGTPSVLTMTEIKKIISLFKTSQKTEITFEINPNDTTEKYLKELKQ
                     LGINRISIGAQTFNDKILKLIGRKHNSKQIKIAVKTAQKAGFENISVDLIYGLPTQTV
                     CDFEKDLQKAVELNIQHISLYGLKIEAGCFFAKKQPASLPDSDTQADMYLAAIKILQK
                     NNFKHYEISNFSKKGFKSKHNLNYWNNNNYYGFGLSASGYENKTRYTNQTDLKKYIKN
                     PLLKITKDKLTEKEILEEAIFLGFRKIPGINTKKINKNFNINFGDKYKTILDKYLISG
                     HLKKTKIGFALTRKGILVSNSVLCEFLS"
     gene            complement(6810..8273)
                     /locus_tag="PKI94_06480"
     CDS             complement(6810..8273)
                     /locus_tag="PKI94_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26419.1"
                     /translation="MTSLINSELNNKNVFSQFTTSKNSPVNARNYYVPSTQNLYNTDN
                     ITQEKKSHALGYSIALTTLVVGFGLLALMKGLPKSFYQKTDKLYKYFDEKTTTLAARM
                     KMLKSKNQKLPKLGNLYLDALKGARYMVEFAKGGFTFASLKDVIVGRAFIKMKKNKTL
                     NKVPLLNKFHPWITNLFEKISTKTVKNAYTKTSGIFGEMRWKFKKTNEIIEKSPLKQG
                     MSFERKNELIKILDEKIQKVKTLYDEGFDRKTVNERLELMGKNLGNMNEKVWEKTFGN
                     LKEFLKDRKSYTTFIQEELAAPIKISHSQQVNHLKDSLVGEKGEIQEILDIYKELLPE
                     KDFLKLKKIAHKSIKNLNKSTNLETDKLFDKIRDLKIGSAPNDSLSILASLGLIGWGL
                     GKADNNDQRLSVALKYGIPALGAIATALYCTVSLVAGGPAIVFGLVSGLVINKIGTIA
                     DNARKRYEDKTLTIKNIAQDNLPKIDLNQNLFNYSKQ"
     gene            complement(8353..9483)
                     /gene="lpxB"
                     /locus_tag="PKI94_06485"
     CDS             complement(8353..9483)
                     /gene="lpxB"
                     /locus_tag="PKI94_06485"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNW26420.1"
                     /translation="MTKKIFIITGEHSGDIHASKVVDELKKIYPDVIIEGVGGENLKN
                     AGVKLFSTHEKMSVMGISLKILINHLNLGKNIINYLKNEFKPDLVLLVDYGAFNLNIA
                     KVLKKTKLNCKIFYYVPPQIWASRKWRINVIKKNVDKVLCIFPFEKPMYDEYKIENHY
                     CGHPLTMELPPKADREEFFEKHELDKNKKLVSIFPGSRIFELENLMKTFVKSAKILQN
                     KHPELQILISQAPNLSDEVFKKHLKNTDFKVIKGENHAMLSVSDALVLASGTVALEAA
                     LYETPMIIAYKGPWLLYLIYLLVRCLDKVSLPNIISNKLVVPEILQEKLSAENICYNI
                     EKLLYDKKSREEQIEHLKEVKLLLSDKNSPYEAALEIKSALM"
     gene            complement(9485..10300)
                     /gene="lpxI"
                     /locus_tag="PKI94_06490"
     CDS             complement(9485..10300)
                     /gene="lpxI"
                     /locus_tag="PKI94_06490"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNW26421.1"
                     /translation="MSLMLEHKQGLIAGDGLLPVKLAQYAKENGFEVIGISLSSDNYN
                     QLKKYCSKVYSVAPGEVLKIQKILETEEIKQLTFLGKVHKGLLLKRPKFDTKAIELVR
                     NASKLNDDEVMLTLIGELEKVGVTVLDQTIFIKNLMIPAGVLGKYNPTQSQMDDVNYG
                     YAMAKEMGKLDIGQSVVIKNKMVMAVEAIEGTDKCIRRGAKLAKGGACVVKVAKPSQD
                     KRFDIPTVGLKTLKTMKKYGATLLSVEANETIIVDQEKTIKFADKNKIVIMAV"
     gene            complement(10354..11091)
                     /gene="kdsB"
                     /locus_tag="PKI94_06495"
     CDS             complement(10354..11091)
                     /gene="kdsB"
                     /locus_tag="PKI94_06495"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNW26422.1"
                     /translation="MTGQTAIIIPARYDSTRLKGKPLIIVNDKPIIQWVFEKAVKSKL
                     ADRIIIATDNQEIYNTAIMFGAEAEMTSDTHQSGSDRIAEVVEKYPEISYIVNLQGDE
                     PLITPESIDQIIKGVKDDKDADISTLVRVLTDKKEIENPNCVKCARDLNGYALYFSRS
                     KIPYERNEGHSKIYGHLGIYGYKREALLKMTKLPQTSLELAESLEQLRALQNGMKIKT
                     FVVDFSPVGIDTAEDLEKFKEIVSQST"
     gene            complement(11088..11984)
                     /gene="prmA"
                     /locus_tag="PKI94_06500"
     CDS             complement(11088..11984)
                     /gene="prmA"
                     /locus_tag="PKI94_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNW26423.1"
                     /translation="MDNYYELKIKINPEASEIVSNICFENFECEGVILAEETYKDLEL
                     VSTTEGILKAFLKSDKNIKSVLQKNRNLLLKRGFSNKDLGCWDFEIIKIENQDWSKKW
                     KENWDVTHVSDKISVVPSWIKYESKPEETVISLDPGSAFGTGTHPTTQLCMRAIEKYL
                     KINDKMADIGTGSGILAICAIKFGAISAYGCDNDETVIHTAQNNSENNAVSNKCHFEY
                     NTAGRIKNKFDFITANILHNVLADIMVDLKVLMKDNATLVLSGILNEKKDIVLDSIAK
                     HGLELIEQTQQENWVALTVRKK"
     gene            12101..12598
                     /locus_tag="PKI94_06505"
     CDS             12101..12598
                     /locus_tag="PKI94_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26424.1"
                     /translation="MKIGKIGGTFLLISSVLTYACGRAPLRKASVQDVAPKIVQTINS
                     LSMKTRNSVSADSSLVEFGVDTLMINTNLTKNVPKFINWLNENGRKHVQKVETGYHLE
                     TRLMYMNGKWIPMPFRVVDYSDKYIPSSVKTVAAEQLYIDKNKILYLPVEYYGKPNPK
                     ITNGF"
     gene            complement(12607..13560)
                     /locus_tag="PKI94_06510"
     CDS             complement(12607..13560)
                     /locus_tag="PKI94_06510"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNW26425.1"
                     /translation="MAAILEFEKPIMEIQEKIEELKKISSESGMDLNKEIETFEKQAK
                     DYKKELYANLKPAQKLLIARHPNRPSFLDYVNLITDDFIELHGDRAGMDDRAIIGGIA
                     KFNGKPIMLIGTQKGKTTKENLAYNFGMPQPQGYRKALKLFNHANKFNMPIVTLIDTP
                     GAYPGITAEETGQGIAIAVNLREMAKFNVPIVAVITGEGCSGGALGLAVADKVLILEH
                     AYYTVISPEGCASILWRDGSMTNTAAEALKITGPDLMQFNIVDGIIKEPLGGAHCNHE
                     EIAAELKKAIGESLDELKKFSPKELKENRYNKFRNLGVVEG"
     gene            complement(13644..14510)
                     /gene="accD"
                     /locus_tag="PKI94_06515"
     CDS             complement(13644..14510)
                     /gene="accD"
                     /locus_tag="PKI94_06515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNW26426.1"
                     /translation="MTIQSWFEKRKQAQEARKALEAKIEDDGLPGLWTKCVHCQAQLP
                     KKDLEDNDMVCPLCDYHFRINAKTRITQLFDKKSFKEMFAEVKPTDPLNFVDTESYKE
                     RIQKAKDKTGLDEAVITGIGSIEGHRVAAAVMDFDYMGGSMGSVVGEKVTRIMEEAIK
                     QRIPMLAICSSGGARMQESALSLMQMAKTSCACAKMDEEKILYITLLTEPTFGGVTAS
                     FGTLGDIIVAEQGARIGFAGRRVIEQTIRQKLPSDFQTAEYLLKFGQVDVIAKRKDLK
                     STLARIIEMHKG"
     gene            complement(14583..>15326)
                     /locus_tag="PKI94_06520"
     CDS             complement(14583..>15326)
                     /locus_tag="PKI94_06520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNW26427.1"
                     /translation="IMRWADNLMPLKFYIAPFRFYSKKLEDYKYRQLVMQALDVWQKA
                     SGGKVSFVIVDTLMQSQINLDWKRVDRQAFGHCYFHFDGQNRLYSAEVQIGITDGLIH
                     QDYMPETEVYHTILHEIGHALGLGHSPNEKDIMYTPHKYGIVNLSKGDKLTLQWLYKF
                     KLNAEVSEIASKHGIPSPNLDKIVATILENNPQSEFEKVKNSISQEKRDLLEEQEKIA
                     DLKKYNIALQNIKISEDIRKIFIPPIQKN"
BASE COUNT         4345 a         2674 c         2742 g         5565 t
ORIGIN      
        1 ggtaacatgg gacttacgat agcagaaaaa ataatttcaa ggcatgcaaa taaggaagtt
       61 gaagcaggcg aacttgtcat tgcaaatgtc gatgtttgtg cggttcaaga cggaacagga
      121 cctttgacgg tacaggaatt taaaaaaata ggtgctaaaa aattaaacaa ccctcaaagg
      181 acaattttat ttatagacca tgcttcgcca agcccgagaa aagaactttc aaactctcat
      241 aacgtattaa gagaatttgc taaagaatac ggtgccgttt tatcagatgt aggagccggc
      301 gtttgtcatc aaagattgat tgaagattac gttaatccgg gtgaaatttt aataggtgct
      361 gattcccaca cctgcacgtc aggcgcattg ggtgcttttg caacaggcat gggctcaact
      421 gatgtagcag tcggaatggc tttgggtaaa acctggctta aagtgccgca aactttcaaa
      481 attcaagttg atggagaatt caggcaagga gtttgttcaa aagatttgat gctttatttg
      541 ataggcttaa tcggtgccga tggcgcaact tataaggctt tggaattttg cggaagcacg
      601 attgacaata tgtatatggc tgaaagattt acgcttgcaa atatggcagt agaggctggg
      661 gcaaaagcgg gactttttgc gacagatgaa aaaacaaaag aatatttgca atcaagaggc
      721 agaggagata aatttgttga gattaagccc gatgttgatg ctcattatga aagagttata
      781 aaaatcgatg cttcaatagt aggtcatatg gtttcctgtc ctcacacagt agataacgtt
      841 aagccagctt ctgaattaag ggatgtaaag gttaatcagg tttttgtagg aacttgcacc
      901 aatggaagaa ttgaagattt aagaattgct gccaatatct taaaaggtaa aaaaattcat
      961 ccggaggtga gaatgttggt ttcgcctgca tcaaaagaag tatttaaaaa ggcacttaga
     1021 gaaggactta ttgatatttt tgtagatgca aatgcaacga ttttagcccc cggttgcggt
     1081 ccttgcgtag gagttcatgc aggaatttta gcagataatg aagtttgtct tgcaactcaa
     1141 aacagaaatt ttcaaggcag aatgggcaac acaaaaagct ttatttattt gtcgtcgcca
     1201 tatgttgcca cttgtagtgc aataaaaggg tttattactt cttcttcttt ttagtcaatt
     1261 cctgtacaat atggcttgcc ataaaaatcc ccgcagcaga cgctacaaaa ggtgttgagg
     1321 caggagtaat ttttgtatat tcaatttttt cgccgcgttg agtttcaaca gtttcagtgt
     1381 ttaatatttt attctgacac attggctttt catcacttgt tacaaccatt atgccctcgt
     1441 taatgtttct tagtttaagt tggtgaatga tatttgaaat aaaaggcgtt tttttatttt
     1501 taatttttga tatgtcgcaa atataaagtc cttcgggatt aaccctgttt cctgcgccga
     1561 atgaacttat cacagggaat ttttcagtat aggcaaactc caataaagaa acttttgagc
     1621 ggattgtgtc aatcgcatct gcaataaaat caatattttt gcctattaat atgctgttta
     1681 agctttcggt ataaaagtca tttatcaaat gaacattgat ttcaggatta atgtctttga
     1741 gccttttttc aaaaagtgtc gttttttcct gacccaaagt agaattcaga gcaacaattt
     1801 gtcgattaat atttgaaaca gaaactttgt caaagtcaac gagagttaat tccccaatgc
     1861 ccgctcttgc caacatttca acacaatatc cgcctactcc gccgagtcca aaaacgatta
     1921 tgtggctttc ggacaatttt ttttgtcctt ccttgcccca ataaagttca tttctggaaa
     1981 aaatatcatt cattatttaa taatgccgaa gcctaacaga aacgtgcaca aaagaaatac
     2041 cgcagcgaca taaccggtta atttgttaag ccccgcttcc gcgctttttt gcgaagaaaa
     2101 aagttggctg gctccgccga ttccagcaat tccgtcacct tttggtgaat ggagcaatat
     2161 aagaacaatc agcaaaattg ccgaaaatat ttgtactatc caagtaaatg taagcatttt
     2221 ttctccttaa agttaatctt tttttgtttt tggttttgaa ataaagtggg ctctttttga
     2281 gtttaagctt attttctcaa aagtgtaaag tcttgccgga cgttttgatt ttgattttga
     2341 gaattcgttt aattctgtta atccgtctgt gcttaaaact ttttttctga aatttctttt
     2401 gtccagtttt ttgttcatta tgagttcgta agctttttgc atttcggtga gtgtgaattt
     2461 ttcatttaaa agctgataag ctacagggca catttcaagc ctttcgcgag ttcttttgag
     2521 cgagtattcg ataatttctt tgtggtcgaa agccaacggc ggaagttcat aaaccctgtg
     2581 ccaggataca tcgggcgaac ttatgatatg aacatcttct tctcttataa gtgcaaaata
     2641 tgcacaagtt ataacccttg cgtctgtacg tctcagcggg tcgccgaaag tataaagctg
     2701 ttcgatataa atgttttcaa cattagtttt ttcttttaaa attctcaatg ccgcttcatc
     2761 aaggttttca gacaaacgaa taaatcctcc gggtatagcc catttgcctt taaacggctc
     2821 attagtacgt tttacaagca ataccataag tttgttgtct ttgattgtta aaataacaac
     2881 gtcaactgta atttcgtggg ttttggtttc gttaaacata tcacctcatt ggtcggacta
     2941 ctttaattct agtataaaaa taaaaatagt gcaaaaatga ggaaacgtta agtaaaaata
     3001 cttaacgttt cctcattttt atttttcaat tttttagact acgccttgag atggcagagg
     3061 ctcttgagtt tgaacatttg tggtttcaat ctctttgact tcaactgcct tgacttcggg
     3121 ttttttctct tcaagctgtt ttagttcgac ctgcttaata tcttgtttca tatttttgac
     3181 ttttagtatt gcagggtcag gattaatttt ttgcctgtta tattctttca taatctttga
     3241 tacaaagctt cttgtttcgc ctatcggagg gacttttttg tatcttttta ccgcgccggg
     3301 acctgcgttg taagctgcaa gtgcaagttc ggttgagcca aacatgttat aaaggcgttt
     3361 gtaatacatt aaaccgcctt taatgttatc gcttaagtag taaggattga cccctaacct
     3421 ttttgctgtt gacggcataa gctgaaaaac tccgaccgca ccgtatctgc ttcttgcttc
     3481 gtgcctgaag cctgattctg attttgctat gctgagtgcc aacgcaggat caatccccag
     3541 ctcaagagag tgctttacga ttgtctcttt aactgttgat acgctatgtt gacttgcatt
     3601 gactttaggg catagtaata gcactattgt aatagtgtat aatactaatt ttctaatcat
     3661 gtaaatcctc taatattgta aattgcattc ctccggacat aaaaaagttg tagtcatttt
     3721 ctatgttttt cccctttgga aaaaagtttg taatgatatc ttaatacata aagaaaaaaa
     3781 atcaagtcca agaagttttt tcatgctata atgtcttgtg taaaagcttt ttttaattgg
     3841 cttttgcaga aatgtaaact tttttaaaaa agggatggtt ttatgaaaaa caagtttgga
     3901 tttacgttgg cagaagttat ggttgtgttg gtaactttag gtgttttggc agctattgct
     3961 attcctgcgg tagttcatgt ccggccgaat aataaaaaag ttatgcttaa aaaggcatat
     4021 tccacgttgg aaaaagctgt acaagaattg attaacaatg atacatatta tccttatgag
     4081 gcagatgacg gaaatacggg agctcaatat ctttgggggc tctcaacaga cattacgccg
     4141 attcgtgtat ggaaaggatt taattacgtt tacaataaag aagctctagg tattccgata
     4201 ggggataata aattttgtta tcttttagca aacagtatga atacagtagg cgcgattgat
     4261 tgtgctatat ttggaacgca tacgagtatg agtgatgcga attttaaaac tgcagacggg
     4321 attacatgga ctatgctcac aaaccaagca gctgatgctc aattcccttt gaatcagaat
     4381 gattatttta atatatcggt ggatgttaac gggaccaaag atcccaactg cggatctggt
     4441 gtatttacaa cgcagtcgtg tgcaagttca ggaccggacc aatttgactt ttcagtcagg
     4501 tacgatggta aaatcaaggt tagtaacgct gcgggtataa ttataactga cccggcaact
     4561 aatcactaga atttgaaaaa atacattgca tgggagagga aaatgtcaga aaaaaaacaa
     4621 ttagcaaaaa taggagtatt cggggggtca gggttttata aattcctgga taatattgaa
     4681 gaaataaggg ttgaaacccc ttatggaatg ccaagcgata atatatttgt aggtaaactc
     4741 ggcaacaaaa aagttgcatt tatgccgcgt catggcagaa atcacagcat tatgccgcat
     4801 ttaatcaatt acagggcaaa tgtttgggct atgaagtcaa tcggggtaga acgggtgatt
     4861 tctccttgtg ctgcaggaag tctgcaaaaa catgttgtgc ccggtgattt tgtaatttgt
     4921 gaccaatttg ttgactggac agacggaaga aaatctacca cgattgaagg gccgattgtt
     4981 tcacatcctt cgcctgctga cctgtattgt cctgaaatga gaaatcttgt tattgataca
     5041 gccaaaaaac ttggtgttaa tgtccattct acaggtactg ttgtcgtaat aaacggaccg
     5101 agattttcta caaaatcaga atcaaaattc tttaccaatc agggctggga agttattaat
     5161 atgacggctt ttcctgaaaa ttatctcgta agggaaatgg atatgtgtcc gcttaatata
     5221 tcacttataa ctgactatga tgcagggctt gtcggtgatg taccgcctgt ttctcatcat
     5281 gcggttatgg aagtttttaa ctccaattta gatagactta aaaaggttct ttttacaatg
     5341 attgagcaaa ttcctgatga aagagaaaat tgtgaatgtt caaaaaccag tgtaaattca
     5401 agactttaga aaacttagga gagaacaatg tacaccgtta aaattgttga taatattttt
     5461 tatttctttt atttgataat tattttgagg attttcttaa catggattcc taatatcgat
     5521 tgggagcagc agcctataaa agctataaga gaagttacgg atatgtattt ggatattttc
     5581 agaagattta ttccgccaat aagcggtttg gatttttctc ctataattgc tttgattttt
     5641 ctgcaaatta ttcagatagt tgtagttaat atagtttctt caatcttagg ataaaaattc
     5701 gcaaagaacg gaattgctta caagaatacc ctttcttgta agtgcaaaac ctattttcgt
     5761 ctttttcaag tgccctgaaa ttaagtattt gtcaagtata gtcttatatt tatccccaaa
     5821 atttatatta aaatttttgt ttattttttt tgtgttgatt ccggggattt ttctgaatcc
     5881 cagaaaaatc gcctcttcaa gaatttcttt ttcagtgagt ttgtcttttg ttatttttaa
     5941 aaggggattt tttatgtatt tttttaaatc cgtttgattt gtgtatctgg ttttgttttc
     6001 atatccactt gccgataatc cgaaaccata gtaattgttg ttattccaat aattaagatt
     6061 gtgttttgat ttaaatcctt tttttgaaaa attgcttatt tcatagtgtt taaagttgtt
     6121 tttttggaga atttttatcg ctgccaaata catgtcggct tgggtatctg aatcaggcag
     6181 tgaggcgggc tgtttttttg caaaaaaaca gcccgcctca attttaagcc cgtagagcga
     6241 aatgtgttga atatttaatt caacagcttt ttgtaaatct ttttcaaagt cacagacagt
     6301 ttgtgtaggg agtccgtaaa ttaaatcgac gctgatattt tcaaaacctg ctttttgagc
     6361 ggttttaact gctattttaa tttgttttga attatgcttt cttccgatta gctttaaaat
     6421 tttgtcgtta aatgtttgag caccgatgct tattctgttt attccaagtt gttttaattc
     6481 ttttaaatat ttttctgttg tatcattagg atttatttca aaagttattt ctgttttttg
     6541 agatgtttta aaaagtgaaa ttattttttt gatttctgtc atagttagga cagagggcgt
     6601 tccgcctcca aaataaaggg ttttaagaaa ttcaccctta tatatagttt taatttcata
     6661 cattaaagct tcaagataag cttttttcaa atcaattttg tcaaaagaaa caaaggaaca
     6721 gtaattgcat ttactcctgc aaaaaggtat gtgaatataa gcatttttag tcataaaatc
     6781 attttattct aaaaaacact ttggtcaaac tactgtttag aataattaaa aaggttttga
     6841 tttaaatcaa ttttaggaag attatcttgg gctatgtttt taattgtcag cgttttatct
     6901 tcatatcttt ttcttgcgtt atccgcaatt gttcctattt tatttatcac caatcctgat
     6961 acaagaccga atacaatggc aggaccgccg gcaacgagac tgaccgtaca gtaaagtgct
     7021 gttgctattg ctccaagtgc aggaataccg tattttaaag ctactgatag cctttggtca
     7081 ttattgtctg ctttgcctaa tccccatccg ataagcccca aagatgccaa aatacttaaa
     7141 gaatcgttag gcgcagagcc tatttttaaa tctcttattt tgtcaaataa tttgtctgtt
     7201 tctaaatttg ttgatttatt aaggttttta attgatttat gcgcgatttt ttttaatttt
     7261 aaaaagtctt tttcaggcag cagttcctta taaatgtcca aaatttcttg tatttctcct
     7321 ttttctccaa caagtgaatc ttttaaatgg tttacttgtt gcgaatgcga tatttttatc
     7381 ggagctgcaa gttcttcttg aataaatgtt gtgtaacttt ttctgtcttt taaaaattct
     7441 ttcaaattcc cgaaagtttt ttcccagact ttttcgttca tatttcctag atttttgccc
     7501 atcaattcta atctttcgtt tactgttttt ctgtcaaaac cttcgtcgta aagggttttt
     7561 actttttgta ttttttcatc aagtattttt atcagttcat tttttctttc aaagctcatc
     7621 ccttgcttaa gcgggctttt ttcaataatt tcatttgttt ttttaaattt ccacctcatt
     7681 tctccaaaaa taccggatgt ttttgtgtag gcgtttttaa ctgttttggt ggatattttt
     7741 tcaaacagat tggtaatcca aggatgaaat ttatttaaaa gaggaacttt gtttaatgtt
     7801 ttattttttt tcattttaat aaaagctctg cctacaataa cgtcttttaa agaagcaaaa
     7861 gtgaagcctc ctttggcaaa ttcaaccatg tatctggctc ctttaagggc atctaaatat
     7921 aaatttccca atttaggaag tttttgattt ttggatttta gcattttcat tcttgctgcc
     7981 agtgttgttg ttttttcgtc aaagtatttg tataacttat cggttttttg gtaaaaactt
     8041 tttggaagtc ctttcattaa tgctaaaagg ccaaagccta cgactaaggt tgttaatgca
     8101 atactgtaac ccaatgcatg actttttttc tcttgtgtga tattgtccgt gttgtaaagg
     8161 ttttgcgtcg aaggtacgta ataatttctc gcattgacgg gcgagttttt actcgttgta
     8221 aactgcgaaa atacattttt attatttaat tcagaattta taagactagt catctacaca
     8281 aaaccttctt gtttttatta aaacaaaaaa gccatataaa ttgctttttt attgggttta
     8341 aataaacatt tgttacatta atgcggattt gatttctaaa gctgcttcat aaggtgaatt
     8401 tttatccgaa agaagcaatt taacttcttt taaatgttca atctgttctt ctcttgattt
     8461 tttatcgtat aaaagttttt ctatattgta gcaaatattt tccgcactaa gtttttcctg
     8521 caaaatttca gggacaacaa gtttatttga aattatgttt ggaagcgaaa ccttatcaag
     8581 gcaccttaca agcagataaa tcaggtataa aagccatggc cctttgtatg ctattatcat
     8641 aggtgtttca taaagagcag cttctaatgc taccgtgcct gatgcaagaa ctagagcatc
     8701 agaaacactc agcattgcat gattttcacc ttttattact ttaaaatcgg tattttttaa
     8761 atgttttttg aaaacttcat ccgataaatt aggtgcttgt gaaattaaaa tttgaagttc
     8821 gggatgttta ttttggagaa tttttgcaga ttttacgaat gttttcataa gattttcaag
     8881 ctcaaaaatt cttgagccgg gaaaaataga aacaagtttt ttatttttat ccaattcgtg
     8941 tttttcaaaa aattcttctc ggtcggcttt aggcggaagt tccattgtaa gcggatgtcc
     9001 gcaataatga ttttcgattt tgtactcatc gtacataggt ttttcaaatg ggaaaataca
     9061 cagaacttta tcaacattct ttttaatcac gttaattcgc cattttctgc tcgcccaaat
     9121 ttgaggcggg acataataga aaattttgca gttaagtttt gttttcttca aaacttttgc
     9181 gatattaagg ttaaatgccc cgtaatcaac cagcaatacc aaatccggct taaattcgtt
     9241 ttttaaataa tttatgatat ttttgccaag attaaggtga tttatgagaa tttttaaaga
     9301 aattcccatt acagacattt tctcgtgtgt agaaaaaagt tttacgccgg catttttaag
     9361 attttctccg ccgacacctt caataatcac gtcaggataa attttcttaa gttcatccac
     9421 taccttgctt gcatggatat cacctgagtg ttcacctgtt ataatgaaga ttttcttagt
     9481 cattttaaac cgccataatt acgattttat ttttgtccgc aaattttatt gttttttctt
     9541 gatccacgat aattgtttcg tttgcttcta cgctcaaaag agttgcacca tattttttca
     9601 ttgttttaag tgtttttagt ccgactgtgg gaatgtcaaa tctcttgtcc tgagatggct
     9661 ttgctacttt cacaacgcag gctccgcctt tggcaagttt tgcccccctg cgtatgcact
     9721 tgtctgttcc ttcaatggct tcaactgcca taaccatttt atttttaatt acaacggact
     9781 gtcctatgtc aagttttccc atttcttttg ccattgcata gccataatta acatcatcca
     9841 tttgtgattg ggtgggattg tatttgccga gaacgcctgc cggaatcatt aaatttttaa
     9901 taaaaattgt ttgatctaaa acagttactc cgaccttttc aagttctcct ataagcgtaa
     9961 gcataacttc atcatcatta agttttgagg catttctgac taattctatt gcttttgtgt
    10021 caaatttagg acgttttaag agcaggcctt tatgtacttt gcctaaaaag gtaagctgtt
    10081 ttatttcttc ggtttcaaga attttttgaa ttttaagcac ttcaccaggg gctacagaat
    10141 aaactttcga acagtatttt ttcagttgat tgtaattgtc tgaactgaga gatatcccaa
    10201 ttacttcaaa tccgttttct ttggcatact gcgccaattt gacaggtaaa agcccgtccc
    10261 ctgcgattaa gccctgctta tgttccaaca ttaatgacat caaaaataat tctccctaat
    10321 ctattttcat caattatact ataaaaaaca actttaagtt gattggctta ctatttcttt
    10381 aaatttttcc aaatcttctg cggtgtctat tccaacagga ctgaaatcca ccacaaaagt
    10441 tttgattttc attccgtttt gaagtgctct taattgctcc aagctttcgg caagttccag
    10501 cgaagtctga ggaagttttg tcatctttaa aagagcttct cttttgtagc cgtaaattcc
    10561 caagtgcccg taaatttttg aatgaccttc gtttctttca taagggattt ttgagcgcga
    10621 aaaataaagc gcataaccgt ttaaatccct tgcacatttt acgcagttgg gattttctat
    10681 ttctttttta tcggttaaaa ctcttacaag agtggagatg tcggcatctt tatcatcttt
    10741 aacacctttt attatctggt caatgctttc gggtgtaatt aagggctcat caccttgaag
    10801 gtttacaata taagaaattt cgggatattt ttcgacaact tctgctatcc tgtcgcttcc
    10861 gctttgatgt gtgtcggaag tcatttcggc ttctgctcca aacataattg cggtattata
    10921 aatctcttga ttgtctgttg caataattat tctatctgca agctttgact taacggcttt
    10981 ttcgaaaacc cattgtatta tgggcttatc gttaactata atcagcggtt tgccttttag
    11041 tcttgtagaa tcatatcttg ccggaattat tattgccgtt tgtcctgtca ttttttcctc
    11101 acagtcagag ccacccagtt ttcttgttgg gtttgttcaa ttaattctag tccatgtttt
    11161 gcaatagagt caagcactat atcttttttt tcattcaaaa tcccgcttag aacaagagtg
    11221 gcattatctt tcattaagac ttttaaatcc accataatgt ctgcaaggac attgtgaagg
    11281 atgttagccg ttatgaagtc aaatttgttt tttatcctgc ctgcagtgtt atattcaaaa
    11341 tgacatttat tcgaaactgc gttattttct gagttatttt gcgcagtgtg aattacggtt
    11401 tcatcattat cacatccgta tgcggatata gcaccgaatt ttattgcaca tattgcaagg
    11461 attcccgaac ccgttccgat atctgccatt ttatcattga tttttaaata cttttcaata
    11521 gcgcgcatgc aaagctgtgt tgtgggatgt gttccagtcc cgaaggcaga gcccgggtct
    11581 aaagatatta ctgtttcttc aggttttgac tcatatttaa tccagcttgg tacaaccgaa
    11641 attttatcag atacatgcgt aacgtcccaa ttttctttcc atttttttga ccaatcctga
    11701 ttttctattt ttataatttc gaaatcccaa cagcctaagt ctttattgct aaaccctctt
    11761 tttaaaagca ggtttctgtt tttttgcaaa acagatttaa tgtttttgtc ggattttaaa
    11821 aatgctttta aaatgccttc ggttgtactt acaagttcaa ggtctttata agtttcttcg
    11881 gctaaaatta ccccttcgca ttcaaaattt tcgaaacaaa tatttgaaac aatttctgag
    11941 gcttcagggt taattttgat ttttaattcg taataattat ccattttaat tattcatata
    12001 tgtttagtaa aaatttagca caattgttaa agaatgtaaa taaagttaat tttattgtca
    12061 aaaactaacg gcgtttattt ataatacaaa aggagaaaaa atgaaaatag gaaaaattgg
    12121 cggaacattt ttattaatat cctcggtgct tacttatgct tgtgggcgtg cccctttacg
    12181 caaggctagc gttcaggatg ttgcgccgaa aattgttcag acaataaatt cactcagtat
    12241 gaaaacccgt aattctgtat cggctgattc aagcttggtt gaattcgggg ttgatacttt
    12301 gatgattaat accaatttga caaaaaatgt tcccaagttt attaattggt taaatgaaaa
    12361 cggtagaaaa catgttcaaa aggtagagac aggttatcat ttagaaactc gtcttatgta
    12421 tatgaatgga aaatggatac ctatgccttt tagagttgtt gattattcgg ataagtatat
    12481 tccaagtagt gtaaaaactg tagccgcaga gcagctatac attgataaaa ataaaatttt
    12541 atatttgcct gttgaatatt atggaaaacc aaatcctaaa ataaccaatg gattttagat
    12601 aaaaatttac ccttctacta ccccaaggtt tctgaattta ttatagcgat tttcttttaa
    12661 ttcttttggc gaaaactttt taagctcatc aagagattcg cctattgctt ttttaagttc
    12721 ggcggctatt tcctcatgat tgcagtgagc accgcccaag ggttctttaa ttattccgtc
    12781 aacgatatta aactgcatta agtctggacc cgtgatttta agtgcttctg ccgcagtgtt
    12841 tgtcattgag ccgtctcgcc aaagaattga agcacagcct tcaggtgaaa tgaccgtata
    12901 gtaagcgtgt tcaaggatta aaaccttgtc tgcaaccgca agacctaatg cacctcccga
    12961 gcagccttcg cctgtaatta cggctacaat cggcacgtta aattttgcca tttctcttaa
    13021 attgacggca atggctattc cctgacccgt ttcttctgct gttattcccg gatatgctcc
    13081 gggcgtgtcg attagggtta caatcggcat attgaactta ttggcgtggt taaaaagttt
    13141 taaagctttt ctgtaaccct gaggctgagg catgccaaaa ttataagcta aattttcttt
    13201 agtggttttg cctttttgtg tgccaataag cattatgggt ttgccgttaa atttcgctat
    13261 accgccgatt atggctctgt catccattcc tgctctgtca ccgtgaagtt caataaaatc
    13321 atctgtaatt aagtttacat aatcaagaaa actgggtctg ttaggatgtc ttgctatcaa
    13381 aagcttttgt gcgggcttta agtttgcata aagttctttt ttgtaatctt tagcctgttt
    13441 ttcaaatgtt tctatttctt tatttaggtc catgcctgac tccgaactga tttttttaag
    13501 ttcttctatt ttttcttgta tttccattat aggtttttca aattctaata ttgctgccat
    13561 ttatattacc ccccatataa gtaaaaagca atcgtgccgt tttacagttt attgtttcca
    13621 tttactattt actatttact tccttacccc ttatgcattt caattattct tgcaagtgtt
    13681 gattttaagt cttttctttt tgcaatgaca tcaacctgtc cgaatttcaa aagatattct
    13741 gcggtttgaa aatctgaagg aagtttttgc ctgatagtct gctcgataac cctgcgtcct
    13801 gcaaagccta ttcttgcacc ttgttcggca actattatgt cgcccagtgt gccaaaactt
    13861 gcggtgactc caccgaaggt gggttctgta agaagagtga tgtacaagat tttttcttca
    13921 tccattttgg cgcaagcaca cgaagttttt gccatttgca ttaagcttag tgcgctttct
    13981 tgcattctgg caccgcccga tgagcaaata gcaagcatag gaattctttg cttaatagct
    14041 tcttccataa tccttgttac tttttcaccg acaacactgc ccatagaacc acccatatag
    14101 tcaaaatcca taacagcggc ggcgactctg tgaccttcga tactcccaat gcctgtgatt
    14161 acggcttcat caagtcccgt tttgtctttt gctttttgaa tacgttcttt gtaagattcc
    14221 gtgtcaacaa aattcagcgg gtctgtcggt ttaacttccg caaacatttc tttgaagctt
    14281 tttttgtcaa aaagctgtgt aattcttgtt tttgcattta ttctgaaatg ataatcgcac
    14341 agcggacaga ccatgtcgtt gtcttcaagg tcttttttgg gtaattgtgc ttggcaatgt
    14401 acacattttg tccataaccc cggcaaaccg tcatcttcta tttttgcttc aagtgctttt
    14461 ctggcttctt gggcttgctt gcgtttttca aaccaagatt gtattgtcat tatttattac
    14521 tcctcattta ttcaaaacat tatacatcaa aaaaaaatcg gtcaagaaat ttggacattt
    14581 gtttaatttt tttggatagg cggaatgaat atttttctga tatcttccga gatttttatg
    14641 ttttgcaaag caatattata tttttttaaa tccgcaattt tttcttgttc ttccagtaaa
    14701 tccctttttt cttgcgatat tgagttttta accttttcaa attcgctttg aggattattt
    14761 tctaaaattg ttgcaacaat tttatcgaga tttggtgaag gaataccatg ttttgaagca
    14821 atttccgata cttccgcatt aagtttgaat ttataaagcc attgtaaggt tagtttatcc
    14881 ccttttgata aatttacaat gccgtatttg tggggagtgt acatgatatc tttttcatta
    14941 ggactgtgtc cgagtccgag tgcgtgtccg atttcgtgca aaatcgtgtg atatacttcg
    15001 gtttcaggca tgtagtcctg atgaataagc ccgtctgtta ttcctatttg aacttctgcc
    15061 gaataaagcc tgttttgtcc gtcaaaatga aagtagcaat gcccgaaggc ttgcctgtca
    15121 acccttttcc aatcaaggtt aatttgcgac tgcataagtg tatctacaat tacaaacgaa
    15181 actttacccc cgcttgcttt ctgccagaca tcaagggctt gcattacaag ttggcggtat
    15241 ttgtagtctt caagcttttt tgaataaaac cgaaacggcg caatgtaaaa ttttaggggc
    15301 attaaattat ccgcccagcg cataat
//
LOCUS       DAOQAU010000041        15254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_4195_length_15254_cov_8.253109, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000041 DAOQAU010000000
VERSION     DAOQAU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 15254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15254
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4195_length_15254_cov_8.253109"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..76)
                     /locus_tag="PKI94_06525"
     tRNA            complement(4..76)
                     /locus_tag="PKI94_06525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Val,seq:tac)
     gene            complement(249..1109)
                     /locus_tag="PKI94_06530"
     CDS             complement(249..1109)
                     /locus_tag="PKI94_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26428.1"
                     /translation="MQVQSINSNINRVKFGSEEQFDKDLSFINLDDIQLSRLSLLKAK
                     EEQGKKRNPLKSLLITLPLVDIASKFIMQSGGIVHDGNINIHNRTYEKFKILPGSLSK
                     KMSAAGHAAMKWGGVLLVLGIYNSIKDAVVSKSEKLQNIEKNHPFASMLADIGLFLGS
                     LSLAEKGANKIAEKMVIKTPDLLEKVGKKVSSAKVWLDKTSINTKVLPKVSELFGRLS
                     KKSPLLATFGLVAVAAAPYIVIVNSLFKISKQNRDFNNNYLNNYRNMKEGQLQLSKTY
                     LNKVVKEFNT"
     gene            1241..2512
                     /gene="mtaB"
                     /locus_tag="PKI94_06535"
     CDS             1241..2512
                     /gene="mtaB"
                     /locus_tag="PKI94_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNW26429.1"
                     /translation="MKTFNLKSMGCKSNQLEGLLIEQNLIKNGFEKMPLNQADFFVLN
                     SCTVTHKSDNEAFSILQKVKRENPDIKTVLTGCIAQIEKENLLKTSFIDFVVGNDEKL
                     NISEVLNLKKSFSVKNIFELKDFHKVLLDDTTKTRASVKIQDGCDNRCSYCIIPFARG
                     KNRSADLDFILKQIKIYEQANFKEIMLTGIHIGQWRVNDSENNLLNLLRSIEEKTNIS
                     RFRLGSLNPLEIDDEMLEFLKNSQKFCPHFHLSIQSMCDKTLKTMNRHYTVAKTLDLI
                     EKIHKNFDAPFIGSDIIAGFAGEGDEDFQITVENLKKSKLTQIHTFPYSVRKGTAAEK
                     FENQVDLKTKNKRAAVIKQISAQKHEEFLQKNIGAIQEVLIEKNLDKKTGFLKGMTRN
                     YINVLLDPQKYNFEKNTLYKIKIEKEHLVLG"
     gene            complement(2737..3870)
                     /gene="ftsZ"
                     /locus_tag="PKI94_06540"
     CDS             complement(2737..3870)
                     /gene="ftsZ"
                     /locus_tag="PKI94_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNW26430.1"
                     /translation="MENFGPDIFGKKEVLQQKPVINPASIKVVGVGGGGGNAVNRMIK
                     AGLSGVDFWLMNTDLQVLSYSSCKNKLQLGAKLTNGLGAGGEPQIGEKAAEEAQQEIT
                     EALEGADMVFVTAGMGGGTGTGAAPIVAKIAKELGILTIGVVTRPFSFEGKRRQNQAD
                     QGLEKLRESVDALIVIPNDKLLDVVDRRVSLQESFIVVDEVLLRGVQGISDIITIPGL
                     INVDFADVKSVMQASGSALMGIGRGQGEGRAIDAARTAIDSQLLETSINGASGVIVNI
                     TGGPDMTLHEVTDATNIINSAVLDDAKVIIGAVVDDKIQGEIQITVIATGFELRNQSP
                     MSEKADSKTLSAADFFSGAFTQKKVADFTPGLGNIEIPDFLKK"
     gene            complement(4108..5193)
                     /locus_tag="PKI94_06545"
     CDS             complement(4108..5193)
                     /locus_tag="PKI94_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNW26431.1"
                     /translation="MIEFDKIPQSSKIAIYGAGEAGLCVKNYIENSRSDLKIVYFFDE
                     KIKGKINEIEIKHIKDIQKFANSFDFIIPASFSNSFLMESILKHYGIQNCIRLNEIPK
                     TNNEFKNNEPRLNIVKNILHSEESKKIFELVFNAYINSNNGVYLFEYLKNFNEQKFHS
                     KGQYFDFIVHEKIKTVISGGAAEAYTTTAFLNSFNNLKKIYAFEPIYQSFKIEQNDLF
                     LKNSGKVEIIEKGLFDKSTVTNFLLNKASSRISQHFPSELTQEIEIVSIDEFVKQKNI
                     EKVDFIKMDIEGAELMALKGAKETLKKHRPQLAICIYHSYDDLFEIPIYLDGLLEKYK
                     FEVYHYSPNTNLETVLYAIPKELNSER"
     gene            complement(5276..6760)
                     /locus_tag="PKI94_06550"
     CDS             complement(5276..6760)
                     /locus_tag="PKI94_06550"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNW26432.1"
                     /translation="MDVKVLNGELKTQKCDLLVVGLFEKTKTLSGVISTVDKALGGII
                     TKFVIQKDKFPAKSKETYLLQTYDKIPARKVLIVGLGDKKKLTSDTIREIGAKIVAKA
                     QSIKAKKICVDLGELNIDAQESGYTFALGATIGEYSFDKYKSEKNKDKIEELILVEKN
                     KAKFEKLKKGADKGIKIAQAMNFARNLSNEPAQEVTPSKLAQVATKIKGTKGITVKIY
                     EKEEIEKMGMGAFLAVGKGSSQPPKFIHIKYTPAKPKKKIALVGKGITFDSGGLDIKP
                     ASSMLTMKDDMSGAACVLSIMNTIKDFSPNVEVHAIVAACENMPGCSAYKPGDVLVAK
                     NKKTIEIDNTDAEGRLTLADALCFASELGVDEIIDIATLTGACMVALGTQAAGIMGNN
                     QKLIDNLIKIGSQSGERLWQLPIWDEYKDTLKSDVADIKNSGGRWGGASSAGVFLKEF
                     VKKEIKWVHLDIAGVAFLESSQKELCKGATGAGIRTILNYILSI"
     gene            7179..8699
                     /gene="rny"
                     /locus_tag="PKI94_06555"
     CDS             7179..8699
                     /gene="rny"
                     /locus_tag="PKI94_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNW26433.1"
                     /translation="MSLLLTLTIVLVIAFSAVIVVQQNKFKMLSRQSGKTLADFSERE
                     DILKKEAIIAAKEAIQEEREKLNEEIRERRQEISRIETKLAQKEDTLETKTQEVTEKE
                     SQLQRKKEELLEKEDYLAELVQKQTSELERISGMSAEDAKNMLFEQLKHDLAQEQMQL
                     IRENEAKIKENALEKSKEILTTTMQRCMIDTTVESTVSVVALPSDEMKGRIIGREGRN
                     IRTLETLTGVDLIIDDTPEAVVLSSFDPVRREIAKLALEKLIADGRIHPVRIEEMVEK
                     AKEEIDKKILEEGENAAMDMGIMGLNKELIRTLGRLYYRTSYGQNVLKHSLEVGHIAG
                     MLAAEIGANVYVAKRAGLLHDIGKAVDQTQEGTHIQLGVELARKFGEKEEIIHAIEAH
                     HDDVTANTIEAVLVKLADAISAGRPGARRDTLEIYIKRLEKIEEIVKSYEGIKQSFAV
                     QAGREVRVIVQAEQFDDLASQKLARDVAQRIENELDYPGQIRVTVVREFRATEIAK"
     gene            8838..9656
                     /locus_tag="PKI94_06560"
     CDS             8838..9656
                     /locus_tag="PKI94_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNW26434.1"
                     /translation="MSTQLKIIFFGDIVGKLGRKAVGDYLAELKGKGDAPDFCIANAE
                     NASHGFGLIEKNYNELVGYGIDCLTSGNHIWDKKDIFDYIKRADKLIRPLNYPPKTKG
                     VGSKIFEIGKTKIAVINLLGRVFMPPIDSPWEIVKKEIKKLKKITPIILIDFHAEATA
                     EKICFARFCADLNVSAVFGTHTHVQTADEKIINNMAYITDAGFCGVTESVIGMEIETS
                     LKRLTTCLPERYEIPKGKIVQINAVEVLIDKKSGKALDIKRISFEKDYTLGGEK"
     gene            9653..10507
                     /locus_tag="PKI94_06565"
     CDS             9653..10507
                     /locus_tag="PKI94_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNW26435.1"
                     /translation="MKSDLHIHTNYSDGVFKPEQIVDSAIETGLDVIALTDHDNVLSW
                     QVAQDYLKTLHTKSLEIIRGVEINTLYNNYEIHILGYFMDVENADFKNLIKIQQQARI
                     KQTKEIVTLLNKKESIRITLEDITNQVAQGGSVGRPHIAKAITSAGGTTSVIEAYAKY
                     INDKSPVYVQRKTVTPHDAVEVIYDAGGIPVLAHPHDLDIAESLVKELMHYGLRGIEA
                     YHRKHSPAIVEYFSSMAEELGLIVTGGSDFHAPNVMNGQIILGKNFVPEWVYDKLLAE
                     KKHLDLAR"
     gene            10520..11155
                     /locus_tag="PKI94_06570"
     CDS             10520..11155
                     /locus_tag="PKI94_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26436.1"
                     /translation="MNKNGFTIIEVVIVFLLILGVTFFVLPLSLNNTKQAQFISKWVN
                     TYSGLEYVFSSIKAQNGFDLGKKFKHAKNNDYKTAVMLDIIKPYLRFKSGVPSSEYKP
                     LYMNKKTVIQGDRYYFDNLYFWDDDKIFGLKWISDNCKENDVCASMLVDINGITLPNT
                     WGKDIFGVNIFSDKLEPMGKNIERNVLKNDCSPFGFGVYCSYYYLIGGEFD"
     gene            11148..11768
                     /locus_tag="PKI94_06575"
     CDS             11148..11768
                     /locus_tag="PKI94_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNW26437.1"
                     /translation="MTNGKKDYKAIKNVCVFSSASNHLDKIYYETAKELGVLMAQAGF
                     NLVYGGGTLGTMGVNAKAIKEFGGAVYGVLPEKLYNIGVGNADCDELFVTECMRTRKD
                     KLDKISDAVIALPGGFGTLEELTEMIVQKQLGYNQKAIVILNTNGFYDNLLNFFNDII
                     KQNFVHALMEDLYYVAKTPKEAIDYLKNFKPTDENILKKLELAAKS"
     gene            complement(11955..12947)
                     /locus_tag="PKI94_06580"
     CDS             complement(11955..12947)
                     /locus_tag="PKI94_06580"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW26438.1"
                     /translation="MTLNTVYETRALTKDLYKIFAGVYSDFRAVAVENYKFELEPLSY
                     EGFVEAVEKELIKCIVLLENQIPTAFLVYTTMISEAIELNVIHCLGEEDLTEKRKVLV
                     EKFLEETQEERTKQVVCYPMLGSQGDFVEYLSNYGFKFVGLAVLRFMFQNSNSEAILN
                     NMELSELEEGYEIKTWLSGYFHDAVKIVQAAFENTADALFDPRFKSIDGTEDILEKIV
                     NSVYGEFLPMATSVLTYKNQPCGFCFANVTGGKIANIPIVAIEKEHRGKGLSKHLLKK
                     TIKTLLHSNKTGEREFSEVNTCTETNNYQALKMYRHAGFKEDYSYPQSYLGANE"
     gene            complement(13000..14223)
                     /locus_tag="PKI94_06585"
     CDS             complement(13000..14223)
                     /locus_tag="PKI94_06585"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNW26439.1"
                     /translation="MNENEIKEGFFDNIEEAIEDFKAGKMLIVADDENRENEGDLICC
                     AQKVTPEIINFMATECKGLICLAIDDETAKRLELSQMVEKNTESANTAFTQSIDGAAK
                     FGVTTGISAFDRAKTIQIAIDKNSRPNDLRRPGHIFPCVAKKGGVLERTGHTETSVDL
                     AKIAGQAPAGVMCEILNPDGTMARRDELRKFADKYNIKFITVEQLISYRIKKEKFVTR
                     EAQATLPTQFGDFKVLGYLNNLTQEEHVALVKDDGSDKIPLIRVHSECLTGDVFHSLK
                     CDCNSQLHRAMKMVDDWGRGAIIYIRNHEGRGIGLINKIKAYSLQEKGQDTIEANISL
                     GFEPDLRDYGVGAQIILDLGYNNFNLITNNPKKIVGLKGYDLKIHEIVNLEPDLNCYN
                     QKYMATKKEKMHHMM"
     gene            complement(14225..14866)
                     /locus_tag="PKI94_06590"
     CDS             complement(14225..14866)
                     /locus_tag="PKI94_06590"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNW26440.1"
                     /translation="MFTGIIEETGTIIEFKKGSSDAFIKVECKKVLENTKIGDSIAIN
                     GVCQTVIESGKNYFCAQISAQTLSLTTFSSAKTGDKVNLERALTLKDRLSGHFVSGHV
                     DGTAKVLSIQKQKDFYNMAFEIPQSLSKYVVNQGSITFNGISLTVAQIILNEVEVAII
                     PHTFENTNLKTFKIGDFVNLECDILAKYVEKNLSTENNKSKLSESFLQENGFF"
     gene            complement(14898..>15254)
                     /locus_tag="PKI94_06595"
     CDS             complement(14898..>15254)
                     /locus_tag="PKI94_06595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW26441.1"
                     /translation="GRLTTIEFYEKRIELALDNFKKCQVDDVITIKQGSATAILKELE
                     EEFDFVFLDADKSEYVKYFEIINSKLKKGGIIAADNITSHAKKVAPFVDKIKSDPDYQ
                     VEVLDLPAGLLVAYKL"
BASE COUNT         4849 a         2840 c         2639 g         4926 t
ORIGIN      
        1 atttgggcgt tgagaggctc gaactcccga catcttcctt gtaagggaag cgctctacca
       61 actgagctaa acgcccgcac tattcaattt tttcggagat acctttaccc ccaactgagc
      121 taaacgccct aataaatgaa aagtgaaaag ggtttttatt tttctcatca ttcattatct
      181 aaataaaaaa gcgtcattta acgctttctt gcttaaatat tattaaaaac aaacttattt
      241 gtcaaatttt atgtattaaa ttcttttaca actttattta aataagtttt tgaaagctgt
      301 aattgtccct ctttcatatt tctataatta ttcaaataat tattgttaaa atcacgattt
      361 tgttttgaaa ttttaaacaa gctgttaact ataacaatat aaggtgctgc ggcaaccgct
      421 accagtccaa acgtagccaa taatggtgat tttttactta atcttccaaa aagttctgaa
      481 acttttggca aaacttttgt attaatcgag gttttatcca accaaacttt tgctgatgaa
      541 acctttttac cgactttttc taaaaggtca ggagttttta ttaccatttt ttcagcaatt
      601 ttatttgcgc ctttttcagc taacgataaa gaacccaaga aaagccctat atctgcaagc
      661 atagaagcaa aaggatgatt tttttcaatg ttttgtaatt tttctgattt tgaaactact
      721 gcatctttaa tggaattgta aattcccaaa accaataaaa ctccgcccca cttcattgca
      781 gcatgccctg cggcagacat ttttttagat aaacttccgg gtaaaatttt aaatttttcg
      841 tatgtcctat tgtgaatatt tatattacca tcatggacaa taccgccgct ttgcattata
      901 aattttgatg caatatcaac caagggcagc gtaattaaaa gcgatttcaa aggatttctt
      961 ttcttaccct gttcttcttt agctttcaac aaagataatc ggcttaattg aatatcatcc
     1021 aaatttataa aagataaatc cttatcaaac tgttcttccg aaccgaattt tactctgtta
     1081 atgttgctat ttatactttg tacttgcatc gatacactcc gaattaaaaa atctacatca
     1141 ttataacact ataaacccca aaagtaaata attttgctag ttgatttttt tgttgtcata
     1201 attctttaca tatatttttt ttcttgataa tgtcttattt atgaagactt ttaacctaaa
     1261 atcaatgggc tgcaaatcta accaactgga ggggttatta attgagcaaa atttgattaa
     1321 aaacggcttt gaaaaaatgc cgcttaatca agcggacttt tttgtgctta attcatgtac
     1381 ggttacgcac aaaagcgata atgaagcctt tagtatcctg caaaaggtaa agcgcgaaaa
     1441 tcccgatata aaaactgttt taaccggctg catcgctcag attgaaaaag aaaacctgct
     1501 taaaacttct tttattgatt ttgtagtggg aaatgatgaa aaattgaata tatcagaagt
     1561 tttgaactta aaaaaatctt tttctgtaaa aaatattttt gaactaaaag attttcataa
     1621 agtcttgctc gatgacacaa ccaaaacaag ggcgagcgta aaaattcaag acggatgcga
     1681 caaccgctgc tcttactgca ttatcccttt tgctcgcgga aaaaacagaa gtgcggactt
     1741 ggattttatt cttaaacaaa taaaaattta cgaacaagca aactttaagg aaataatgct
     1801 tacggggata catattgggc agtggagggt aaatgacagt gagaataacc ttttgaactt
     1861 gcttagaagc attgaagaaa aaacaaatat ctctcgtttt cgtttaggct ctttaaatcc
     1921 tttggaaatt gacgatgaaa tgcttgaatt tttaaaaaat tctcaaaaat tctgcccgca
     1981 ttttcacctt tcaatccaat ccatgtgcga caaaacctta aaaacaatga acagacatta
     2041 tacagttgcc aaaacacttg atttaatcga aaaaatacat aaaaactttg acgccccttt
     2101 tatcggaagc gatattattg caggctttgc aggtgaaggc gatgaagatt ttcaaataac
     2161 agtcgaaaat ttaaaaaaat caaaactaac ccaaattcac acctttcctt attctgtgcg
     2221 aaaagggact gccgccgaaa aatttgaaaa ccaagttgat ttgaaaacaa aaaataaaag
     2281 ggctgcggtt atcaaacaga tttcggcgca aaagcacgaa gaatttctgc aaaaaaatat
     2341 cggtgcaatc caagaagtct taattgaaaa gaatttggat aaaaaaacag gatttttaaa
     2401 agggatgaca agaaattaca taaatgtttt gcttgaccct caaaaataca attttgaaaa
     2461 aaatacgctt tataaaatta aaatcgaaaa agaacatttg gttttaggat aaaaaagggc
     2521 ggttttctga aagaaaaccg ccctttttaa tattttttat tattatcttg gattattcgg
     2581 gtacgcatag tccttcattc ccttttcaaa attgctttcg caattttagc actccatcgg
     2641 actatttgct cttaacgggc tgggctcttc ctctcacaaa tgatatttaa tcatttgctc
     2701 ggcagagtgc tcgcacggtg ccctaccgaa aatccgctat tttttcaaga aatcaggaat
     2761 ttcaatattt cccaaccccg gagtaaaatc tgcaactttc ttttgagtaa aagcacccga
     2821 gaagaaatca gctgcactca aagtttttga atcagctttt tcactcatag gagattgatt
     2881 tctcaattca aaacctgtcg caataaccgt gatttgaatt tccccttgaa ttttatcatc
     2941 aacaaccgca ccgataataa ctttagcatc atcaagaact gcagagttaa taatgtttgt
     3001 agcatctgtt acttcgtgaa gcgtcatatc aggtccgcct gtaatattaa caattacgcc
     3061 gcttgcgcca ttgattgaag tttccaaaag ttgagaatcg atagctgttc ttgcagcatc
     3121 gattgctctt ccttcgccct gaccgcgtcc gatacccata agcgcagagc ctgaggcttg
     3181 cataacactt ttaacatcgg caaagtcaac gttaataagc ccgggtattg tgataatatc
     3241 agaaatacct tgaacaccgc gaagaagaac ttcatcaact acgatgaatg attcttgaag
     3301 agaaactctt ctgtcaacaa cgtcaagtaa tttatcgtta ggaattacaa tcagcgcatc
     3361 aacgctttct ctaagttttt ccaaaccttg atcggcttgg ttttgtctgc gttttccttc
     3421 aaaagaaaac ggtcttgtaa caacgcctat tgtcaaaatt ccaagttctt ttgctatttt
     3481 agcaacgata ggagcagcac ccgttccggt tccgccgccc atgcctgctg taacaaaaac
     3541 catatcagca ccttcgagtg cttctgttat ttcctgttga gcttcttcag ccgctttttc
     3601 accgatttgg ggctctccgc ctgcgccgag tccgtttgtc agtttagcac ccaattgaag
     3661 tttatttttg caagaagaat aagacaagac ttgcaagtca gtgttcatca gccaaaaatc
     3721 tactccgcta agccctgctt taatcatacg gttaacggca tttccgccgc cgccgcctac
     3781 accaacgacc ttgatgcttg caggattaat tacgggtttt tgttgtaata cttctttttt
     3841 tccaaagata tccggtccaa aattttccac tatattattc ctctttttgt tattacttac
     3901 cacaatatat tatcatacta ttttaaagaa aaaaaatagt aaagaacatg tatcaaaatt
     3961 attatccaaa tttaatataa gttcaagaaa cttcgcattt tgtaaagaaa ttattacaaa
     4021 tcagtgaaca gttaactgtg aactgtgaac tgtctggcac gtttcgcgca tctttctctt
     4081 gattttaaat ttttaccatt ttacatctca ccgctcactg ttcaactcct tcggaattgc
     4141 atataaaaca gtttctaaat ttgtgttcgg agaatagtga taaacttcaa atttatattt
     4201 ttctaaaagc ccatccaaat aaatcggaat ttcaaacaaa tcgtcataac tgtgataaat
     4261 acatatcgca agctgcggac gatgtttttt taaggtttct ttcgcccctt ttagtgccat
     4321 taattccgca ccttcaatat ccatttttat aaaatcgact ttttcgatat ttttttgttt
     4381 tacaaattca tcaatcgata cgatttctat ttcttgtgta agttcagaag gaaaatgctg
     4441 acttatacgg gaagatgctt tattcaataa aaaatttgtt accgtagatt tatcaaaaag
     4501 ccctttttct attatttcga ctttacctga atttttcaaa aaaaggtcat tttgttcaat
     4561 tttaaaactt tggtatatag gttcaaatgc atatattttc ttcaaattat tgaaactgtt
     4621 taaaaaggct gttgtggtat aagcttccgc cgccccgccg cttataacgg ttttaatttt
     4681 ttcatgaaca ataaaatcaa aatactgccc tttggagtga aatttctgtt cgttaaaatt
     4741 ttttaagtac tcaaacaaat aaacgccatt attagaatta atataagcgt tgaatacaag
     4801 ttcaaatatt tttttactct cttcggagtg tagaatattt ttaactatat ttaatcgcgg
     4861 ttcgttattt ttaaattcat tatttgtttt tggaatttca tttaatctta tacaattttg
     4921 tattccgtaa tgttttaaaa tggattccat taaaaaagaa tttgaaaaag aagccggaat
     4981 tataaaatca aaggagtttg caaatttttg aatatctttt atatgtttaa tttcaatttc
     5041 gttgattttg cccttgattt tttcatcgaa aaaataaact atttttaaat cagaacgaga
     5101 gttttcaata taatttttaa cacataaccc ggcttcgcct gcaccataaa ttgctatttt
     5161 agaactttgg ggtattttgt caaattctat cattttaatt cctataaaaa ctgattaaaa
     5221 aataatatca taaataaggg cggaaaaatc aaagattttt ccgcccttaa aaaaattaaa
     5281 tcgacaaaat ataatttaaa attgtcctta tacctgcgcc tgttgcacct ttgcagagct
     5341 ctttttgaga actttccaaa aaggcgaccc ctgcaatgtc aaggtgtacc catttaattt
     5401 cttttttaac aaattcttta aggaaaactc ccgcagaact tgcaccgccc caacgaccgc
     5461 cggaattttt aatatcggct acatcgcttt tcaatgtatc tttatattca tcccaaatcg
     5521 gcaactgcca aagtctttcg cctgattgag atcctatttt tatcaaatta tctattaatt
     5581 tttgattatt tcccataatt cctgccgcct gtgttcccaa agcaaccata caagctcctg
     5641 ttaaggttgc gatatcaatg atttcatcaa caccgagctc tgaagcaaaa caaagtgcgt
     5701 cggcaagggt taatctgcct tcggcatcag tgttatctat ttcgattgtt tttttgtttt
     5761 ttgctaccaa aacatcgccg ggcttgtaag ccgaacatcc gggcatattt tcgcatgctg
     5821 ctactattgc atgcacttct acattaggac tgaaatcttt tatcgtgttc ataatcgaca
     5881 aaacacaagc cgcacccgac atatcatctt tcatagtaag cattgatgag gcgggtttaa
     5941 tgtccaagcc gccgctgtca aaagtaattc ctttgccgac aagggcaatt tttttcttag
     6001 gttttgcggg ggtgtattta atgtgaatga atttcggtgg ctgggaacta ccttttccta
     6061 cagccaaaaa tgctcccatt cccatttttt cgatttcttc tttttcgtaa attttaactg
     6121 ttataccttt tgtaccttta atttttgttg caacttgggc aagctttgaa ggagtaacct
     6181 cttgagccgg ctcattagaa aggtttcttg caaaattcat tgcctgtgcg attttaattc
     6241 ctttatccgc tccttttttg agtttttcaa attttgcttt atttttttct acaagtatta
     6301 attcttcgat tttatcttta tttttttcag acttgtattt gtcaaaggag tattcgccga
     6361 ttgtcgcgcc aagtgcaaaa gtgtatccgc tttcttgcgc atcgatattt aattctccca
     6421 aatcaacgca gatttttttg gctttaatac tttgtgcttt tgcaacaatt tttgcgccga
     6481 tttctcttat tgtgtcagag gttaattttt tcttatcgcc taagcctaca atcagaactt
     6541 ttcttgcggg aattttgtcg taagtttgca aaagataagt ttcttttgat ttagcgggaa
     6601 atttgtcttt ttggattaca aattttgtaa taattccgcc caatgcttta tcaaccgtag
     6661 aaataactcc gcttaaggtt tttgtttttt caaaaagccc cacaaccaac aaatcgcatt
     6721 tttgtgtttt caattcacca tttaaaactt ttacatccat cgcaaaactc ctttcaaact
     6781 aatttgcaca taattatacc atgaagccag aaaagtaaat agtaaatagt aaatggtaaa
     6841 cagaaggcaa gaggcgaaga cgcgtagagg cgtagcagat aattcacgca tccacgcatc
     6901 tacacatcta cacatcttat cttcttataa cctatcgact tatcgactat tcaactaaaa
     6961 tagccctgca ccctttcagc ctttcagcta aacattttgt aacacccctg tacaaatgac
     7021 caaaaatatg ttagcattgt ttcagatgta gtaggataaa tttttggaaa tatatgcaat
     7081 aaattacagt taaaaatata actttaagct cgatttggta atgaaatcgg gcattgcgtg
     7141 tataaaaaaa atagaaatga acgaggtatt gaattgctat gagtttatta ttaactttaa
     7201 caatcgttct tgttattgct ttttctgctg ttatcgttgt tcaacaaaat aaattcaaaa
     7261 tgctctcccg ccaatcagga aaaactctgg cggactttag cgaaagagaa gatattttga
     7321 agaaagaagc gattattgcc gcaaaagaag ctattcaaga agaaagagag aaacttaacg
     7381 aggaaatcag agaacgccgc caggagattt ccaggataga aacaaaattg gcgcaaaaag
     7441 aagacacact tgagacaaaa actcaggaag taacagaaaa agaatcacag ttacaaagaa
     7501 aaaaagaaga gcttcttgaa aaagaagatt atcttgcaga gttggttcaa aaacaaacct
     7561 ccgaattgga gcggatttct ggaatgtctg ccgaagatgc taaaaacatg ttatttgaac
     7621 aattaaagca tgatttggca caagaacaaa tgcagttaat cagagaaaac gaagcaaaaa
     7681 taaaagaaaa cgctcttgaa aaatcaaaag aaatcttaac cacaacaatg caacgctgca
     7741 tgatagatac cacagttgaa tcaacagttt ctgttgttgc acttccaagt gacgaaatga
     7801 aaggtcgcat aatcggccgc gaaggaagaa atatcagaac tcttgaaacg cttaccggag
     7861 ttgacttaat aattgatgat acgcccgaag ctgttgttct ttcaagtttt gatcctgtca
     7921 gaagagaaat cgcaaaactt gctttggaaa aacttatcgc tgacggaagg attcaccctg
     7981 tcagaatcga agaaatggtt gaaaaagcaa aagaagaaat cgataaaaaa attcttgaag
     8041 aaggcgaaaa tgcagccatg gatatgggca ttatgggact taacaaagaa ttgataagga
     8101 ctttgggaag actttattac agaacaagtt acggtcaaaa tgttcttaag cactcgcttg
     8161 aagtaggtca tatcgcagga atgctcgcag ctgaaatcgg tgcaaatgtt tatgtggcaa
     8221 aacgtgcagg acttttgcac gatatcggga aagccgttga ccaaactcaa gaaggtacac
     8281 atattcaact gggcgtcgag cttgcaagaa aattcggtga aaaagaagaa ataattcacg
     8341 ccattgaggc ccaccacgat gatgttacgg caaatacaat cgaagcggtt ctggttaaac
     8401 ttgcagacgc aatttctgca ggacgccccg gcgcaagacg cgatacttta gaaatttata
     8461 ttaaacgtct tgaaaaaata gaagaaatcg taaaaagcta tgaaggtatc aaacagtcct
     8521 ttgccgttca ggcagggcgc gaagtaagag taatcgtaca agcagaacag tttgacgatc
     8581 ttgcttcaca aaaactcgca agagatgttg cccaaagaat cgaaaacgaa cttgattatc
     8641 ccggacaaat aagagttacg gtggtaagag aattcagggc aacggaaata gccaaataag
     8701 gcagttctag gcaagcgaag cgagccgtag aaatgcctta tcccgaactt gtttcgggat
     8761 ctatagagtg ggggttatga aaaataatac ccctcacccc aaccctctcc cgcaagggga
     8821 gagggggagt taaaaaaatg tcaacccaac taaaaataat atttttcggt gatatcgtcg
     8881 gaaaactcgg caggaaagct gtcggggatt atttggcaga acttaaagga aaaggtgatg
     8941 ctccggattt ttgtattgca aatgccgaaa acgcttcgca cggcttcggg ctaatcgaaa
     9001 aaaattataa cgaactggtc ggctacggta tagattgtct gacctcgggc aatcatatct
     9061 gggataaaaa agatattttt gattacataa aaagggctga taaacttata agacctttga
     9121 attacccgcc gaaaacaaaa ggagtcggct ctaaaatttt tgaaatcgga aaaacaaaaa
     9181 ttgctgtgat taatcttctt ggcagagttt ttatgccgcc gattgattcg ccctgggaga
     9241 ttgtgaaaaa agaaattaaa aaactcaaaa aaataacccc tataatttta attgattttc
     9301 atgctgaagc caccgcagaa aaaatttgct ttgcaagatt ttgcgcagat ttaaacgtaa
     9361 gtgcggtttt tggcacgcac actcatgttc agactgcaga tgaaaaaatt atcaacaata
     9421 tggcatatat aacagatgcc ggtttttgcg gggttaccga aagtgttata ggtatggaga
     9481 tagaaacttc cttaaaacgc ttaacgacct gcctgcctga aagatacgaa attcccaaag
     9541 gaaaaattgt tcaaataaac gcggttgaag ttttaatcga caaaaaaagc ggcaaagctc
     9601 ttgatataaa aagaatttcg tttgagaaag attatacgct cggaggtgaa aagtgaagtc
     9661 ggatttacac attcacacaa attattctga cggggttttt aagcccgagc aaattgttga
     9721 ttcagcaatt gaaacaggct tagacgttat tgcgcttacc gaccacgaca atgttttatc
     9781 ctggcaggta gcgcaggatt atttaaaaac actgcataca aaatctttgg aaattattcg
     9841 cggagttgag ataaacacac tttataataa ttatgaaatc catattttag gatatttcat
     9901 ggatgtcgaa aatgccgatt tcaaaaacct gataaaaatc caacaacaag ctcggattaa
     9961 acagacaaaa gaaattgtta cattgttaaa taaaaaagaa agcataagaa taacccttga
    10021 agacataaca aatcaagtag cacaaggcgg aagtgtcgga agaccgcaca ttgcaaaagc
    10081 aattacgtca gccggcggaa caacaagtgt aattgaagct tatgcaaaat acataaacga
    10141 caagtctccg gtttacgttc aaagaaaaac cgtaacccct catgatgctg ttgaagttat
    10201 ttatgatgca ggagggattc ctgttcttgc acaccctcac gacttagata tagcagaaag
    10261 ccttgtcaaa gaacttatgc attacggact taggggtatt gaagcttatc acagaaagca
    10321 ttcacctgcg attgtcgaat atttttcttc aatggctgaa gaattagggc ttatcgtaac
    10381 aggcggttcc gattttcacg cgcccaacgt tatgaacggt caaataattt tggggaaaaa
    10441 ttttgttccg gagtgggttt atgataaact gttggcagag aaaaaacatt tggacttggc
    10501 aagataaaac ggagttttta tgaataaaaa cggatttacg attattgaag tggttatcgt
    10561 ttttttgctt attttagggg taactttttt tgttttgcct ttaagcttaa acaatacaaa
    10621 acaagctcaa ttcatttcaa aatgggttaa tacatattcg ggcttagaat acgttttttc
    10681 ttcaataaaa gcacaaaacg gatttgattt aggaaaaaaa tttaagcatg caaaaaacaa
    10741 cgattataaa acagcggtta tgctggatat aataaaacct tatctgaggt tcaaatcggg
    10801 agtcccgagt tctgaatata aacctttata catgaataaa aaaacagtaa ttcaaggtga
    10861 ccgatactat tttgataacc tttatttttg ggatgatgat aaaattttcg ggcttaaatg
    10921 gatatccgat aattgcaaag aaaatgatgt ttgtgcatca atgcttgttg atataaatgg
    10981 aataaccctt ccaaacactt gggggaaaga tattttcggg gtaaatattt tcagtgataa
    11041 gcttgagcct atgggaaaaa acattgaaag aaacgtttta aaaaatgatt gttccccgtt
    11101 cggattcgga gtttattgtt cttattacta cttgattgga ggagaatttg actaacggca
    11161 aaaaagatta taaagcaata aaaaatgtct gtgtattttc ttcggcaagt aaccatttag
    11221 ataaaatata ttatgaaaca gccaaagagc ttggggtttt aatggcgcaa gcaggtttta
    11281 atcttgtcta tggcggtgga actttaggta caatgggggt taacgccaag gctataaaag
    11341 aattcggagg agcggtttat ggcgttttgc ccgaaaagct ttataatatc ggagtcggaa
    11401 atgccgattg cgatgaactt tttgtcaccg aatgtatgcg gacaagaaaa gataaactgg
    11461 ataaaatttc cgatgctgta atcgctttgc ccggaggctt cgggactttg gaagaattaa
    11521 ccgaaatgat tgtccaaaag cagcttggtt acaaccaaaa agcaatagtg attttaaaca
    11581 caaacggatt ttacgacaat ctgctcaatt tttttaacga tataatcaaa caaaattttg
    11641 ttcatgcgct catggaagat ttgtattatg ttgcaaaaac cccgaaagaa gcgattgatt
    11701 atttaaaaaa cttcaaaccc acagacgaaa atattcttaa aaagcttgaa ttggcggcaa
    11761 agtcttagta aacagtaaat agtaaatggt aaatagtaaa tagaaggtag gaaggcaggt
    11821 tattaaatat tcttagtcac ctagtcactc agtcactttt ataaactttt cacctgttca
    11881 gcctttcagc tattcaccta aaataacctt tcagccattc acccgttcat ccgttaaacc
    11941 tttttgactg ttcgttactc atttgcgcca agatacgatt gcgggtagct gtaatcttcc
    12001 ttaaaaccgg cgtgcctgta catcttaagt gcctgataat tgtttgtttc tgtgcaagta
    12061 ttgacttctg agaattctct ttcgccggtt ttgtttgagt gcaaaagtgt tttaatcgtt
    12121 tttttgagca aatgttttga taagcccttg cctctgtgtt ctttttctat tgcaacaata
    12181 ggaatattgg cgattttgcc gcctgtaacg tttgcaaaac aaaagccgca gggctggttt
    12241 ttataagtca aaacgcttgt cgccatcggt aaaaattcgc cgtaaacgct gtttactatt
    12301 ttttctaaaa tatcttctgt tccgtcaatt gacttaaatc ttggatcaaa aagcgcgtct
    12361 gcagtgtttt caaaagctgc ttggactatt tttactgcat catggaaata tccgctcagc
    12421 caagttttaa tctcataacc ttcttccaac tccgaaagtt ccatattatt taatatagcc
    12481 tctgaattcg agttttggaa cataaacctt aagaccgcaa gtccgacaaa cttaaatccg
    12541 taattggaca gatattctac aaaatcaccc tgagagccca gcatcgggta gcaaaccacc
    12601 tgttttgttc tttcttcttg agtttcttca agaaattttt ctaccaaaac tttgcgtttt
    12661 tcggttaaat cttcttcgcc aagacagtga ataacattca attcaatggc ttctgaaatc
    12721 attgtcgtat agaccaaaaa agcggtagga atttgatttt cgagcaaaac aatacacttt
    12781 ataagctctt tttctacagc ttcgacaaaa ccttcataac tcaaaggttc aagttcaaac
    12841 ttgtaatttt caaccgcaac ggcgcgaaaa tcgctataga ctcctgcaaa tattttatat
    12901 aaatcttttg taagtgctcg tgtttcgtaa accgtgttta aagtcatttt ttcatccttt
    12961 aaattattat tcggattttt attcaaatcc ttttgagaat tacatcatat ggtgcatttt
    13021 ttcttttttt gttgccatat atttttggtt gtaacaattc aaatcaggct ccaagttcac
    13081 tatttcatga atttttaggt cataaccttt aagtcctacg atttttttag ggttatttgt
    13141 tatcaaatta aagttattat agcccaaatc caaaattatt tgtgcaccga ctccgtagtc
    13201 tcttaaatca ggttcaaatc caagcgaaat attagcttca attgtatctt gacctttttc
    13261 ctgaagcgaa taagccttaa ttttattgat gagcccaatt cctctgcctt cgtggtttct
    13321 gatgtaaatt atagcacccc tgccccaatc atcaaccatt ttcatcgcgc gatgcagctg
    13381 tgagttgcag tcgcatttaa gactgtgaaa aacatcaccg gttagacatt cgctgtgaac
    13441 tcttatgaga ggaattttat ccgagccgtc atctttgaca agggcgacgt gctcttcttg
    13501 tgtcagattg tttaaatatc ctaaaacctt aaaatctccg aattgcgtgg gcaaagtcgc
    13561 ttgcgcttcg cgtgttacaa acttttcttt ttttatccgg taggaaatta actgctctac
    13621 ggttataaat ttaatgttat atttgtcggc aaattttctc aactcatctc tacgtgccat
    13681 tgtgccgtct ggattaagga tttcacacat aactcctgcc ggagcttgcc ctgcaatttt
    13741 tgccaaatca acgcttgttt ctgtgtgccc tgttctttca agcactccgc cttttttagc
    13801 gacacaaggg aaaatgtgcc ccggacgtct gagatcgttc ggccttgaat ttttgtctat
    13861 tgcaatttgg attgtttttg ctctgtcgaa agccgaaatc cctgttgtaa caccgaattt
    13921 tgcagctccg tcaatgcttt gggtaaaggc tgtatttgca ctttcggtgt ttttttcaac
    13981 catttgcgaa agttcaagtc tttttgcggt ttcatcatct attgcaagac agataagccc
    14041 tttacattca gttgccataa aatttatgat ttcgggagtg actttttgtg cacagcagat
    14101 taaatcgcct tcattttcgc gattctcgtc atcggcgact ataagcattt tgcctgcttt
    14161 aaaatcttct atcgcttctt caatgttatc aaaaaagccc tcttttattt cattttcgtt
    14221 cattttaaaa aaatccgttc tcttgcaaaa agctttcgct taattttgat ttattatttt
    14281 ccgttgataa atttttttca acatattttg ccaaaatatc acattcaaga tttacaaaat
    14341 cgcctatttt gaaagtttta aggttcgtgt tctcaaaagt gtgcggtatt atggcaactt
    14401 caacttcgtt taaaataatt tgtgctacag tgaggcttat cccgttaaaa gttattgagc
    14461 cttgatttac gacatatttt gaaagacttt gcggaatttc aaatgccata ttgtaaaaat
    14521 ctttttgttt ttgtatgctt aaaacctttg ccgtaccgtc aacatgaccg cttacaaaat
    14581 gtccgctaag cctgtctttt aatgtgagtg ctctttcaag gtttacttta tcgcctgtct
    14641 ttgcacttga aaaagtcgtc agtgaaagtg tttgggctga aatttgagcg caaaagtaat
    14701 tcttacccga ttcaataaca gtctggcaaa caccgtttat agcgatactg tctcctattt
    14761 tggtattttc aagcactttt ttgcactcga ctttgataaa agcatcagac gagccttttt
    14821 taaattcaat tatcgtgcct gtttcttcaa taatccctgt gaacataata tatattttat
    14881 catggaaaaa tttatgatta aagcttataa gcaacaagaa gccctgcggg caaatccaag
    14941 acttcaacct gataatcggg gtctgattta attttatcca caaaaggtgc aacctttttt
    15001 gcatgcgagg tgatgttatc tgctgcaata atgccgcctt ttttgagttt cgaattaatt
    15061 atttcaaagt attttacgta ttctgacttg tcggcatcta agaatacaaa atcaaattct
    15121 tcttctaatt cttttaaaat cgctgtagct gagccttgtt ttattgttat aacgtcatcg
    15181 acttggcatt ttttaaaatt atcgagcgca agctcaattc ttttttcgta aaattcgatt
    15241 gttgtgagcc gccc
//
LOCUS       DAOQAU010000042        13531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5002_length_13531_cov_7.371772, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000042 DAOQAU010000000
VERSION     DAOQAU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5002_length_13531_cov_7.371772"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKI94_06600"
     CDS             complement(<1..535)
                     /locus_tag="PKI94_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNW26442.1"
                     /translation="MKKKAFNLSEVLLAIAIIGIVAALTIPNLNKNVKKSDLETSFKK
                     GYEILSQAITQMQLDNDDSIYTKFTTSPNNTSAKLKTALMPYFNVINNCTPETKCLPT
                     NMTGYKNYTNKTTFMNTSHFVYKFYVENGMLISPIVSSTLIILWVDTNGYKKGPNRAG
                     YDMFAFQILSNDSLIPIG"
     gene            complement(636..1382)
                     /locus_tag="PKI94_06605"
     CDS             complement(636..1382)
                     /locus_tag="PKI94_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26443.1"
                     /translation="MRKLLSVFMALSLLSWNILPVFADDVKINNQCGCGSCPVEQIIA
                     TEHLNINKKAPVNTVKITNYRKIICQNNVLGIEYVCRFSSKKACPGDRVDFIIPENIY
                     SCEGKLIIPACTKIVAEVAGIQQPKWFNKNARVSMNFKYLILPDGRTVCLCARPFTKD
                     GTLKEGPWMTAGRIVLYTVTGGIIGTGAGIGFGFIPSPTKLGVGLAAGIPAGCGIGLI
                     TALVTKGLQYKAKPGEQIYIILGCDACIPN"
     gene            1540..2964
                     /gene="pyk"
                     /locus_tag="PKI94_06610"
     CDS             1540..2964
                     /gene="pyk"
                     /locus_tag="PKI94_06610"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNW26444.1"
                     /translation="MKNFVSTKIISTLGPSSDTKEKIKALVEAGVTMFRLNSSHGDEA
                     QHLRNLGYIREIEQESGQLIPVLLDLQGPKIRVGNFEESIELKNGETIRFRHQENYEN
                     GIIPVDYEGISQDVKIGEKILMDDGKLILMVTGIEGDVVITKVICGGLLHQRKGINIP
                     GSTGSIKVLTDKDVEYVKFAIDNDIDYIGLSFVRNAEDINTLKEVIQSHNGKIKIISK
                     IEKPQALKNIDEILLKSSGIIVARGDLGVEISPEKVPVVQKELIRKANAQRKSVIVAT
                     QMLESMIENPLPTRAEASDVANAILDGTDAIMLSGETAAGKYPVEAVSMMKSIAQNVE
                     NSSLMKHNKYPDDLIDGSTDDSIAICKAVMDMVDKINVKAIMAISESGYTPKLLSKAK
                     PGVPILTICPSKRNSRRMLLYSDVFPVKFDMDESINETNLNLLDGFLIENFKLKTGDK
                     IIFTGAIPRLMAGKTNFIKLHEIS"
     gene            complement(2987..3829)
                     /locus_tag="PKI94_06615"
     CDS             complement(2987..3829)
                     /locus_tag="PKI94_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNW26445.1"
                     /translation="MKRFLEKISIHTILIFVSLLSIFPFIWLISTSLKGNAENIFAYP
                     PQIIPQDFTFQNYVGVWQRVDFIAYFVNSLIVAGATVFLNLILSALAAYPLARMEFNG
                     KKTAFFAVLATIMVPFQAIMLPVYLITLKLNMVDSVNNFMGYLGLVMPFAVSAFGIFL
                     MRQAFLTIPKEMEEAAIVDGCNVFQVFFKVLLPMVKPSLAVLAIFTFIGSWGEFLWPS
                     IVLTKESMYTLPVGVNNLQGMFSSNWRFIAAGSILATIPIVIFFLAMQKYFISGENEG
                     AVKG"
     gene            complement(3826..4161)
                     /locus_tag="PKI94_06620"
     CDS             complement(3826..4161)
                     /locus_tag="PKI94_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNW26446.1"
                     /translation="MSKRDYSDFIYDIIEAIKSINKFIGKMSFEEFSKDDKTVSAVTR
                     KFEIIGEAANRIPKTIQKKYDDIPWSYMIGMRNKIIHDYDAVNLMIVFDSAKEDLKKL
                     LPLLEKMLK"
     gene            complement(4154..4447)
                     /locus_tag="PKI94_06625"
     CDS             complement(4154..4447)
                     /locus_tag="PKI94_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNW26447.1"
                     /translation="MKDKNEILKILKEIKPDLEKDYNITEIGLFGSYLRGEQTKNSDI
                     DILLDHKSGLTFFKLIDLEKFLTNVLGVKVDIAFKKYLKRNIGKKILSEVNYV"
     gene            4645..5100
                     /locus_tag="PKI94_06630"
     CDS             4645..5100
                     /locus_tag="PKI94_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26448.1"
                     /translation="MITISGVTKIFGLSSRTFSGSKVTETPINMLKELYFHIAYDISF
                     NRFNTFHPEFKGHARLPLIGDLSNRLSNVLKEHSHAEDSAVKIIKGLVEEAKTEARTA
                     LRADKSFGLISSESKFLKDCVEQFGADFCEKEQGRIMFQAMQKDSQQLK"
     gene            complement(5411..6280)
                     /locus_tag="PKI94_06635"
     CDS             complement(5411..6280)
                     /locus_tag="PKI94_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNW26449.1"
                     /translation="MNYYKKYVPYLFLLPAGIILLVFFFIPFIQTIILSFKDYSSSIY
                     HPDWVGLQNYFKLMQSPVFYKVLINTFLYLFIAVPILATVPLFLAILINQKIKGITLY
                     KILIYLPVIVSIVVAAIAFKWLYAEQGILNFAMQKMGFESIGWLTDTNFSLISVIIVT
                     IWKGIGYYMMIYLAALMSVPKELYEACEIDGAGFFRKHLTVTIPHIMPTLALVSTISA
                     ISAMKVFAEIYVMTKGGPLNSSKTIVYYIYERAFENLDLGYASAMAVVLLIIVMAFSL
                     INIMCFEKNKYQI"
     gene            complement(6322..6897)
                     /locus_tag="PKI94_06640"
     CDS             complement(6322..6897)
                     /locus_tag="PKI94_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26450.1"
                     /translation="MKKILPVFLFLFFSSTLPVFCDDLGLGDYSQGIDNAWYGQKQIT
                     DEQFEKTLKAVQDKKNKKKNKKKLKGQSLNKYEEGLESSETEDKFKDVFENNAVIGLP
                     VNLVTLEGVQIPVGHYNISGKKINKKVYLNFYQGDLNVASVEATETTNDFGQTSINFA
                     KIIPCDDTKIKIIYGSVDFNAYAYIPIENGI"
     gene            7028..8662
                     /locus_tag="PKI94_06645"
     CDS             7028..8662
                     /locus_tag="PKI94_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26451.1"
                     /translation="MSELFFIILVIIVIVALIFFVYNLMLSSEKPRHEEDLQITSHDI
                     LEQVSILYKQRKYNIVETLAKKYLEKKPSDYGVRSILAKSFYEMGRKYEALEHAHYVC
                     NHQPKNFPMRIFVANCYNDNGKPMQAISVLTDLLNEDANNVVAIKDLAHLYLETNQKK
                     SAIKMLKRLDEFIYSNQEKVNNKNVIARLHMEFREYDEAIEQYQEILQIYPADVLVKK
                     SLVEAYKMNSDYESAIELTEEILSLNINEEANIWATKLLIDLYSVINDYEKALMYANL
                     YKDNPNANKEKATESIAKILMETGQIEESIDVLKNLVENNSKDVELKKTLSRAYESNN
                     NFEAAAEIYKKILDEAGVEDIKNIHLEMSDLYSNWAMFLFNQGDNDACFKKFSLAVQY
                     DVDNPQIYYKLGAINQLIKNYNQAISQYKKAIDLDNSNASYYYSIAECYEAIDNVYEQ
                     KAALLECVKYDIKNSRAYYKLGLLHNVQRDMANAIDCLKKAISIDENFVEAKYKLALL
                     LENQGEVDNAIEIYENILKINPLFEEAANNLKMLKP"
     gene            complement(8664..9302)
                     /locus_tag="PKI94_06650"
     CDS             complement(8664..9302)
                     /locus_tag="PKI94_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26452.1"
                     /translation="MKKFLILLALIFGITATNADVMPYYVNNINSNSIGVYQASHEIK
                     IYKEPNEKSPLLLSVTWDSKNFNSPDISASNLFVVFLPKKDLAFFLVTDENENWVQVI
                     FNRNNSQTGWIKKDDELKFLNWRTFFNNYGRKYGVYYLKDAPEESKTIYSSSDNNGQV
                     LGRIKLPEVIKLTTTKGNWILISVYDIEKIQKIGWIQWRNTNGEIHLFPAIK"
     gene            complement(9322..11784)
                     /gene="pheT"
                     /locus_tag="PKI94_06655"
     CDS             complement(9322..11784)
                     /gene="pheT"
                     /locus_tag="PKI94_06655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNW26453.1"
                     /translation="MQVSLNWLNEFVDLSDIDEKQIAHELTMSGLEVEDVEHFKPQFK
                     NIITAKILKIDTHPNADKLHLVTVDIGKEQRTVVCGAQNIEEGQIIPYASVGSEVFSR
                     KTGEMFTLTPAEIRGVKSEGMLCSSDELGLSDRNYQSEDGILILNRFLKNVELGKPLE
                     EILGLEEDIILHCAPTANRGDEMSVIGIARELCAIFDKELKFSPLESTVDLNTNKFKV
                     EIIDQDVCKYYSIGVLENITIKPSPAWMQKRLIASGIRAISNVVDITNYVLLEYGIPL
                     HAFDLKKLKGYLCVRRAKQGEKITTLDGVERELTTDSVLIATREKGVCLAGVFGGQNS
                     EVDSNTNSIALEAAYFTPASNRKSSHSVGYRSDACARFERGVDIEAVKPALMRAMQLL
                     AEHAGAKVVGMVDDGQNKIEKPEITLRFAQIKRILGCEIPAQRCISILEKLGFKTLGK
                     NEVAAKFEVPSFRVNDVTREIDLIEEIARINGYDKIAPTLPGKNESPIISIEEKTIKQ
                     IHSILLSSGLNEIITSSLIGKPFLTQFSIDYDKEKSVEVENAQSEEFTMLRQTLSANI
                     LNCLKYNYDNGQKNFWAYEIGKIYFKNAPADEKNSGVDEIKMLAGCITGEIENSKWHK
                     TQETNFFTLKGIFEKLFNELGISNRIQFRPCENVDILHPHRSASLVLLGKNLLEIGYM
                     GQIHPILRDRLKLNQEAFLFEVNLEEIIKAINPSITKYKKLPQFPEVQRDLAFIVPKI
                     ISCEELQKAIKKSVQQNIFKNCEIFDVYQGEHIEKGFKSVAFRIKMQDEQATLTDEII
                     DAQMNSIKNNLEKTFEKLTFRV"
     gene            complement(11913..12737)
                     /locus_tag="PKI94_06660"
     CDS             complement(11913..12737)
                     /locus_tag="PKI94_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW26454.1"
                     /translation="MIVDEMEDLYPEVPPTKLRNQEEEFKPARTSPFWLWVANRFFFG
                     MLENRFFALRYKGSEKFEKRDKNIPTIAFAPHTNWWDGIVGYNLCARVFDKEIRLMVE
                     ELNRFPLLRRGGAFSVNKKSAQASMEALTYSVDVIEDLNNVLYIFPQGIIKPPNYRPI
                     EFKTGLAYIIQKAVKKYGKVNLVPIAVNYFFLRADRPEVLVEIGDIIPFDDFKTDRKE
                     LSSKLAKLLENLCDSQNHDVSIGNFKGYRTLFQQKLKWYRKIEQRLKKIEIKGSGV"
     gene            13050..>13531
                     /locus_tag="PKI94_06665"
     CDS             13050..>13531
                     /locus_tag="PKI94_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26455.1"
                     /translation="MQLPLSSLNKPKIGTCPHGLPQGACPICSGMGGGGAKREVKSKP
                     DEMTWDECFAIGQMLKAQKLAQQQKQQAVESQLQVPLNFGAKLANFSAKIAEFSQKVA
                     NFAQKAQGLPSVISKPVVFVLNKLVIPILNFIKDVPVLINKAVNFVKEKLADISDKLN
                     A"
BASE COUNT         4314 a         2296 c         2393 g         4528 t
ORIGIN      
        1 agcctatcgg aattaaagaa tcatttgaca agatttgaaa agcaaacata tcgtagccgg
       61 ctctgttagg acctttttta tatccgttgg tatctaccca aagtatgatt aaagtagatg
      121 aaacaatagg acttattaac attccgtttt ctacgtaaaa tttgtacaca aaatgtgatg
      181 tattcataaa tgttgtttta tttgtataat ttttgtagcc ggtcatattc gttggaaggc
      241 attttgtttc gggagtgcaa ttgtttatta cattaaaata aggcattaga gctgttttta
      301 acttagcact tgtattgttc ggagaagtag tgaattttgt ataaattgaa tcatcgttgt
      361 cgagttgcat ttgagtaata gcctgagaga gtatttcgta acccttttta aaagatgttt
      421 ctaaatcgct ttttttaaca tttttattta aattaggaat cgttaaagca gcaactattc
      481 ctataattgc tattgcaagg agtacttcag ataaattaaa tgctttcttt ttcaatattt
      541 gcacctctct ttaagatatt atacattgta acatgaaatt ttattttaag caaaatgggc
      601 ggcggctttt aagccaccgc cccacttata ttatattaat taggaatgca cgcatcacat
      661 ccgagtatta tataaatttg ctctccgggt tttgctttat attgtaagcc ttttgtaacc
      721 aatgctgtta taagtcctat gccgcagcct gcggggatgc ctgctgcaag tccgactccg
      781 agttttgtag gagaaggaat aaacccgaaa cctatgcctg ctcctgtacc gataatgcca
      841 cctgttactg tgtaaagaac tattcttcct gcggtcatcc agggaccttc ttttaaagta
      901 ccgtctttgg taaatggtct tgcgcaaaga caaacggttc ttccgtcagg taaaattaag
      961 tatttaaaat tcatactgac tcttgcgttt ttgttaaacc attttggctg ttgaattcct
     1021 gcaacttccg caacaatttt tgtgcaggca gggattatta atttcccttc gcaagaatag
     1081 atgttttcgg ggattataaa atctactctg tcgcccgggc aggctttttt agacgaaaat
     1141 ctgcaaacgt attcaattcc taaaacgtta ttttggcaga ttatttttct gtaatttgtt
     1201 atttttacag tatttacagg tgcttttttg tttatgttca gatgttctgt tgcaattatt
     1261 tgctcaacag gacatgaacc gcagccgcat tgattgttaa tttttacatc gtctgcaaat
     1321 acgggtaaaa tattccagct taataacgat agtgccataa aaaccgataa gagttttctc
     1381 attttattca cctctttctt ctgtaaaaac aattaatttt gcccatatta taaactatcc
     1441 tttcaagttt gtaaagtgtc ttggatattg cccgaatgtt ttttttcttg ttgtaattgt
     1501 tcttttaagt taaaataatt ttgaaaaaga gggtttaata tgaaaaattt tgtaagcaca
     1561 aaaataatat ccacactcgg accaagctct gacacaaaag aaaaaataaa agcacttgtg
     1621 gaagcaggag ttaccatgtt tcgcttaaat tcttcccatg gagatgaggc tcagcatttg
     1681 agaaatttag gatatataag agaaattgag caggaatcag gccagcttat tcctgtttta
     1741 cttgatttac aggggccaaa aataagagtc ggtaatttcg aagaatcgat tgagcttaaa
     1801 aacggagaaa caattagatt tcgtcatcag gaaaattatg aaaacggaat tatccctgtt
     1861 gattacgagg gtatttcgca ggatgtgaaa atcggtgaaa aaattcttat ggatgacggg
     1921 aaactcattt taatggttac aggaattgaa ggtgacgttg taataacaaa agttatttgc
     1981 ggcggcttac ttcaccaaag aaaaggtata aatattccgg gctcaacagg aagcatcaag
     2041 gttttaacag acaaagatgt tgaatatgta aaatttgcta tagataacga cattgattac
     2101 atcgggcttt cgtttgtaag aaatgccgag gatataaata cgctaaaaga agttatacaa
     2161 agtcacaacg gtaaaataaa aattatttca aaaatagaaa agcctcaggc acttaaaaac
     2221 attgatgaaa tacttttaaa atcttccgga ataattgttg caagaggcga tttgggtgtt
     2281 gaaatttcac ccgaaaaagt tcctgttgtt caaaaagagc ttatcagaaa ggcaaatgca
     2341 caaaggaaat ctgttattgt cgcaactcaa atgttggaat caatgattga aaacccgctt
     2401 cccactcgcg cggaagcttc tgacgttgca aatgctattc ttgatgggac tgatgcgatt
     2461 atgctctcgg gtgaaactgc agccggcaaa taccctgtag aagccgtttc tatgatgaaa
     2521 tctattgcac aaaatgtcga aaacagcagt ttaatgaagc ataataagta ccccgatgat
     2581 ttaatagacg gctcgacaga cgattcaatc gcgatttgca aagctgttat ggatatggtt
     2641 gataagatta atgtgaaagc cattatggca ataagtgaaa gcggttatac cccgaaatta
     2701 ctttcaaagg cgaagcccgg cgtacctatt cttacgattt gtccaagcaa aagaaacagc
     2761 cgcagaatgc tcttatacag tgacgttttt cctgttaaat ttgatatgga tgaaagtatt
     2821 aacgaaacaa acttaaatct tcttgacgga tttttaattg aaaattttaa acttaaaacg
     2881 ggcgacaaaa tcatttttac aggcgcaatc ccccgattaa tggcaggcaa aacgaatttt
     2941 ataaagttgc atgaaataag ctaaacaatc gattatatat ttacccctag cctttaaccg
     3001 caccttcatt ttcgcctgag atgaagtatt tttgcattgc caaaaagaaa attacaatcg
     3061 gaattgttgc aagaattgag cctgctgcaa taaatcgcca atttgaactg aacattccct
     3121 gtaaattatt aaccccgaca ggaagagtgt acatactttc ttttgtaagc acaatgctgg
     3181 gccacaaaaa ttcaccccaa gagccgataa atgtaaatat tgcaagaact gcaagcgagg
     3241 gttttaccat aggcaaaagc accttgaaaa acacctgaaa aacgttgcag ccgtcaacta
     3301 tagcggcttc ttccatttct ttagggatag tcaaaaaagc ttgtctcatt aagaaaattc
     3361 caaacgcact taccgcaaaa ggcattacca agccgagata tcccataaaa ttatttacgc
     3421 tgtcaaccat atttaatttt aaagtaatca gataaacagg aagcataatt gcctgaaacg
     3481 gtaccataat agttgcaagc accgcaaaga aggcggtttt tttgccattg aattccattc
     3541 ttgcaagagg ataagcagca agtgcagata agataaggtt taaaaaaacc gttgcgcccg
     3601 caactatcaa actgtttaca aaataagcga taaaatcaac tctttgccac actccgacat
     3661 aattttggaa agtgaaatcc tgtggaatta tctggggcgg atatgcaaaa atgttttccg
     3721 cattgccttt taaagatgtg gaaataagcc atataaaagg aaatatcgac agcagcgata
     3781 cgaaaattaa aattgtatgt attgatattt tttctaaaaa tcttttcatt ttagcatttt
     3841 ttctaaaagc ggcaaaagtt tttttaaatc ttcttttgca ctgtcaaata ctatcatcaa
     3901 gtttacggca tcataatcgt gtataatttt atttctcatt ccaatcatat atgaccaagg
     3961 aatatcatca tatttttttt ggattgtttt tggaattcta tttgcggctt cgccgattat
     4021 ttcaaacttt cttgtaacgg cacttactgt tttatcatct tttgaaaatt cctcaaaaga
     4081 cattttgccg ataaatttat taattgattt aatcgcttca attatgtcgt atatgaaatc
     4141 cgaataatct cttttagaca taatttacct cggacagaat ttttttcccg atatttcttt
     4201 taagatactt tttgaatgcg atatcaactt ttactccaag aacattggtt aaaaattttt
     4261 ccaaatcaat cagcttaaaa aaagtcagac cgcttttatg gtcaagcaaa atatcaatat
     4321 cgctgttttt ggtttgttct ccgcgcaaat acgagccaaa caaacctatt tcggtaatat
     4381 tgtagtcttt ttctaaatca ggcttgattt cttttaatat ttttaaaatt tcatttttat
     4441 ctttcataat tagtgattat aacatggcat cgcaaaaatc agcaactgtt aatttttgta
     4501 aatatgagtt ttaagctgct caaacgcagg cgtggcatag aataggatag gataggatag
     4561 gataggatag gataggaatt tttcaggcaa ttttttttct ttagataatt taaaaaattt
     4621 gtaaataatt aaaaggagaa aaaaatgatt acgatttcag gcgttactaa aatatttgga
     4681 ttaagcagta gaactttcag cggatctaaa gtgactgaaa ctccgattaa tatgttgaaa
     4741 gaattatatt ttcatattgc ttatgatata tcttttaatc gttttaatac atttcatccc
     4801 gagtttaaag gacacgcaag actgccgctt attggcgacc tttcaaatag actttctaat
     4861 gttttaaaag aacattctca cgctgaagat agtgctgtta agattatcaa aggtcttgtt
     4921 gaggaagcaa aaactgaagc aaggactgct ttaagagcag ataaaagttt tgggttaata
     4981 agctcagaaa gtaaattttt aaaagattgt gttgaacaat ttggggctga tttttgtgaa
     5041 aaagaacaag gcaggataat gtttcaagca atgcaaaaag attctcaaca attaaaataa
     5101 aacatataaa ctttatctgt agaacgtagg atgggcttca gcccagtaga ataaaaaatc
     5161 aagaggcgaa atttttttta agaaaatttc gcccagtttt tatttacttt ttctttgagg
     5221 agattgcttc ggtggacaat aattataaaa gccgataacc aaagtctcgc aatgacgtaa
     5281 gagtagtaag tgggtttccc aacccatcgt tttttggatt gccgcgtcgt tcggctaaca
     5341 cctcactcct cgcaacgacg tgaacgtagc tagcatcaag ctaaattatt taccccctcg
     5401 caacgacagg tcatatttga tatttatttt tttcaaagca cattatgttt atcagcgaaa
     5461 atgccattac gattatcaaa agcacaaccg ccatagcaga agcgtatccc aaatccaaat
     5521 tttcaaacgc cctttcgtaa atataataaa caatagtttt gcttgaatta agaggcccgc
     5581 cttttgtcat tacataaatt tcggcaaaaa ctttcatcgc agaaattgca cttatcgttg
     5641 aaacaagggc aagcgtcggc attatatgag gaatcgtaac cgtcaaatgt tttctgaaaa
     5701 atcctgctcc gtcaatttcg caggcctcat aaagttcttt gggaacgctc ataagtgctg
     5761 caagataaat catcatgtaa tagccgattc ccttccaaat ggttactata ataactgaaa
     5821 taagcgaaaa atttgtatct gtaagccaac ctatcgactc aaaccccatt ttttgcatcg
     5881 caaaatttaa aatcccctgt tccgcataaa gccacttaaa ggctattgct gcaactacaa
     5941 tcgatacaat tacaggaaga taaatcaaaa tcttgtacaa agttatgcct ttgatttttt
     6001 gatttataag aattgccaaa aacaaaggaa cagttgcaag aatcggaact gcaataaaaa
     6061 gatataaaaa agtgttaatt aaaaccttat aaaaaacagg cgactgcata agtttaaaat
     6121 aattctgaag cccgacccag tcaggatgat aaatacttga agaataatct ttaaagctca
     6181 gaataatagt ttgaataaaa ggtatgaaaa aaaataccag caaaattatt ccggcaggca
     6241 gcaaaaacaa gtatggaaca tattttttat aataattcat caggtcaatt atgacatttt
     6301 ttcaaatttt ggtcaaatat gttaaatgcc gttttcaata ggaatataag cataggcgtt
     6361 aaaatcgaca gagccatata taatttttat ttttgtgtcg tcacagggaa taatttttgc
     6421 aaaatttatt gatgtttgtc caaaatcatt tgtagtttct gtcgcctcga cgcttgccac
     6481 attcaaatct ccctgataaa aattaaggta aactttttta tttatttttt tgcctgaaat
     6541 attgtaatgt ccgacaggaa tttgcactcc ttcaagcgta accaaattaa ccggtagccc
     6601 aataacagca ttattttcaa aaacatcttt aaatttatct tcggtttcgg agctttccaa
     6661 gccttcttcg tatttattaa gactttgacc cttcagtttc tttttatttt ttttcttatt
     6721 ttttttatct tgaacggctt taagcgtttt ttcaaactgt tcgtcggtaa tttgtttttg
     6781 tccgtaccag gcgttatcta ttccttgtga ataatcgccc aatccgagat catcgcaaaa
     6841 aacaggtaag gtggaagaaa aaaataaaaa taaaaaaaca ggcaaaatct ttttcatatt
     6901 gatatcttaa tatatttttt aaaaaagtaa agagggcgtg gaaaaattaa aaaatttttc
     6961 tgcgccctca aggggctttt tttttctatg taaatagtat ataatgtttt tatacggaag
     7021 gttgagtatg agtgaattat tttttattat tcttgtaatt atcgtaatag tggctctaat
     7081 attttttgtt tataatttaa tgcttagttc tgaaaaacct cgccatgaag aagatttaca
     7141 gataacttct catgatattc ttgagcaggt aagtattttg tacaaacaaa gaaaatacaa
     7201 tatagtcgaa actcttgcta aaaaatatct tgaaaaaaaa ccatcagact atggcgtaag
     7261 aagtattctt gctaaatctt tttacgaaat gggcaggaaa tatgaggcat tggagcatgc
     7321 acattatgtt tgtaatcatc aacctaaaaa tttccctatg aggatttttg tcgcaaattg
     7381 ctataacgat aacgggaagc ctatgcaggc tatttctgtt ttgacggatt tgcttaatga
     7441 agatgcaaac aatgttgttg caataaaaga tttagctcat ctttatcttg aaacaaatca
     7501 gaaaaaatcg gctataaaaa tgcttaaaag gttggatgag tttatctaca gtaatcaaga
     7561 aaaagttaat aataagaatg taattgcacg tttgcacatg gaatttaggg aatacgatga
     7621 agcaatcgaa cagtatcagg aaattttaca aatatatccg gcagatgttc ttgtcaagaa
     7681 aagtttggtg gaagcttata aaatgaattc tgattatgaa tcggcaatag agcttactga
     7741 agagattttg tcgcttaaca tcaatgaaga agcaaatata tgggctacaa aacttcttat
     7801 tgatttgtat tcggtcataa atgattatga aaaagcttta atgtatgcaa acttgtataa
     7861 agataatcca aatgcaaata aagagaaggc aaccgaaagc atcgcaaaaa ttcttatgga
     7921 aacagggcag attgaagaaa gtatagatgt tttgaaaaat cttgttgaaa acaattctaa
     7981 ggatgttgag cttaaaaaga ctttgtccag ggcgtatgag tctaataata attttgaagc
     8041 tgccgcagaa atttataaga agattcttga tgaagcaggg gttgaagata ttaaaaatat
     8101 tcaccttgaa atgagtgatt tatattctaa ttgggcaatg tttttgttta atcagggaga
     8161 taatgatgct tgttttaaaa agttttcgct tgcggttcag tatgatgtag ataatccgca
     8221 aatttattac aaattagggg caataaatca gcttataaaa aattacaatc aggcaatttc
     8281 tcaatataaa aaagcaatag atttagataa ttcaaatgca agttattatt attcgattgc
     8341 agaatgttat gaagccattg ataatgttta tgagcaaaaa gcagcacttt tagagtgcgt
     8401 aaaatatgat attaaaaatt caagggctta ttataaatta ggtttgttac acaatgttca
     8461 acgtgacatg gcaaatgcta tagattgctt aaaaaaggca atttctattg acgaaaattt
     8521 tgtcgaggca aaatataaat tggcacttct tcttgaaaat caaggtgaag ttgataatgc
     8581 aatagaaatt tatgaaaata tactcaaaat aaatccttta tttgaagagg cggcaaataa
     8641 tttaaaaatg cttaaacctt aagttactta atggcaggaa acaagtgaat ttctccgttg
     8701 gtatttcgcc attgaatcca gccgattttt tggatttttt caatgtcata aacacttatt
     8761 aaaatccaat tacctttggt agtcgtaagt tttattactt caggtaattt aattctgcct
     8821 aaaacttgtc cgttattgtc gcttgagcta tatatggtct tagattcctc aggggcgtct
     8881 ttaaggtaat aaactccgta ttttctgccg tagttgttaa aaaatgtccg ccagtttaaa
     8941 aattttagtt catcatcttt tttaatccag cctgtttggc tgttgtttcg attaaatata
     9001 acttgtaccc agttttcatt ttcatcggtt acaagaaaaa atgctaaatc ttttttaggc
     9061 aaaaatacta caaaaaggtt gcttgctgaa atatctggcg agttaaagtt tttgctgtcc
     9121 caggttacgc tgagcaatag cggagatttt tcgttcggtt ctttatatat ttttatttca
     9181 tgcgatgcct gataaactcc tattgaattt gagtttatgt tgtttacgta atagggcatt
     9241 acatcggcat ttgttgctgt aattccgaat atcagagcca gtaatatcag aaattttttc
     9301 ataaaaacct ccggttaaaa attataccct gaaagtaagt ttttcaaaag ttttttccaa
     9361 attatttttt attgaattca tttgagcatc aataatttca tctgtcaggg tggcttgctc
     9421 atcctgcatt tttattctga aagcaacgct tttgaaacct ttttcaatgt gttcaccctg
     9481 ataaacatca aaaatttcac aatttttaaa aatattttgc tgaaccgatt ttttaatagc
     9541 tttttgtaat tcttcgcaag aaattatttt agggacaata aacgctaaat ctctctggac
     9601 ttcagggaat tgtggcagtt ttttgtattt tgttatgctt ggattaattg cttttattat
     9661 ttcttcaaga ttaacttcaa acaaaaaggc ttcttgatta agcttgagcc tgtctcttaa
     9721 aataggatga atttgtccca tataccctat ttccaaaaga tttttgccta aaagaaccaa
     9781 gcttgcgctt ctgtgagggt gtaggatatc aacattttca caaggtctaa actgaattct
     9841 gtttgaaatt cccaactcat taaataactt ttcaaaaatc cctttgaggg taaagaaatt
     9901 tgtttcttga gttttatgcc actttgagtt ttcgatttcg cctgtaatgc aacctgccag
     9961 cattttaatt tcatctacgc ccgaattttt ttcatctgcg ggtgcgtttt tgaaataaat
    10021 ctttcctatt tcataagccc aaaaattctt ttgcccgttg tcgtaattgt atttgaggca
    10081 attcagaata tttgcgctta aggtttggcg aagcatggta aattcttcgc tttgggcgtt
    10141 ttcgacttct acggattttt ctttatcgta atcaattgaa aattgtgtta aaaatggttt
    10201 tccgattaat gaggatgtga taatttcatt taatcccgat gaaagcagga ttgaatgaat
    10261 ttgttttatg gttttttctt cgattgaaat tattggagat tcatttttgc ctggaagtgt
    10321 cggtgctatc ttatcgtaac cgtttattct tgcaatttct tctattaaat cgatttcgcg
    10381 ggtaacatca tttaccctga aggaagggac ttcaaatttt gcggcaactt catttttgcc
    10441 taaagtttta aatccgagtt tttccaaaat cgaaatacat ctttgagcgg gaatttcaca
    10501 acctaaaatt cttttaatct gcgcaaagcg tagtgtaatt tcaggttttt cgattttatt
    10561 ttgcccatca tcaaccattc cgacaacttt tgctccggca tgttctgcaa gaagctgcat
    10621 tgcacgcatc agggcaggtt ttacagcttc tatgtcaacg cctcgctcaa accttgcaca
    10681 ggcatccgag cggtagccca cactgtgaga gctttttctg ttgctcgcgg gggtaaaata
    10741 tgccgcttca agtgctattg aatttgtgtt tgaatctact tcggaatttt gtccgccgaa
    10801 aactccagca aggcagactc ctttttctct tgttgcaata agcacgctat ctgttgtgag
    10861 ttctctttca acaccgtcga gagtggttat tttttcacct tgttttgcac gtcttacgca
    10921 caggtagcct tttaattttt ttaaatcaaa agcgtgaagc ggaattccgt attccaaaag
    10981 tacgtagttt gtaatatcaa cgacgttgct tattgctctg atgcctgaag ctataagcct
    11041 tttttgcatc caggcaggag atggttttat tgttatgttt tctaaaactc cgatagaata
    11101 atatttacaa acatcttggt ctattatttc cactttgaat ttgtttgtgt ttaaatcaac
    11161 tgtgctttca agcggcgaaa atttaagttc tttatcaaat attgcgcaaa gctctcttgc
    11221 tattccaata acagacattt catctccgcg gtttgcggta ggagcacagt gtaaaattat
    11281 gtcttcttca agtcccaaaa tttcttcaag cggttttccg agttccacgt ttttcaaaaa
    11341 gcggttaagg attaaaattc cgtcttcgct ctggtaattt ctgtcggata agcccaattc
    11401 atcagatgaa cagagcatgc cttctgattt tacccctctt atttcagcag gggtcagggt
    11461 gaacatttcg cccgtttttc ttgaaaacac ttcagagccc acagaagcgt agggaataat
    11521 ttgtccttct tcaatgtttt gggctccgca aacgactgtt ctttgttctt ttccgatatc
    11581 tacggtaact aaatgcaact tatcagcgtt agggtgggta tcaattttta gaatttttgc
    11641 cgtaattata tttttaaact gcggtttgaa atgttcgaca tcttctactt caagcccgct
    11701 cattgtaagt tcgtgtgcga tttgtttttc gtcaatatca cttaggtcaa caaattcatt
    11761 caaccagttt aaagatacct gcatgtttaa ttccccgcta aaattgttct acattgagtt
    11821 tataataaat taatttcaat gtaaaccatt taacttatcg acttattaac taaaatatca
    11881 ctgctaaccg ctcacggttc actcttcact ttttaaactc ccgaaccttt gatttcaatt
    11941 tttttaagcc gttgttcgat tttgcggtac cattttaatt tttgttgaaa aagggttcta
    12001 taacctttaa aattgccgat tgaaacgtca tggttttgac tatcgcaaag gttttccagt
    12061 aattttgcaa gcttggagga caattctttt ctgtctgttt tgaaatcatc aaaaggaatt
    12121 atgtcgccga tttcaacaag tacttcgggg cggtctgcac gcaagaaaaa gtaatttacc
    12181 gcaataggta ccaagtttac tttgccgtat tttttaactg ctttttggat gatataggca
    12241 agtcctgttt tgaattcaat cggtctgtaa tttggcggtt tgatgattcc ctgagggaaa
    12301 atgtataaga cgttattcaa atcttcaatg acatcgacag aataggtaag tgcctccata
    12361 gaagcctgcg cggatttctt gttcacggaa aatgctccgc cacggcgtaa aagcgggaaa
    12421 cggttaagtt cttcaaccat tagccttatt tctttgtcaa aaactcttgc gcaaagattg
    12481 tatccaacaa ttccgtccca ccaatttgtg tggggtgcaa aagctattgt aggaatgttt
    12541 ttgtcacgtt tttcaaattt ttcgctgcct ttatagcgca gtgcaaaaaa tctgttttca
    12601 agcattccga aaaaaaacct gtttgccacc caaagccaaa aaggcgatgt tcttgcgggt
    12661 ttaaactcct cttcttgatt tcttaacttt gtgggaggga cctcaggata taaatcttcc
    12721 atttcgtcaa cgatcataaa tatatattac taaacttttg ttcatttgtg aatagcaagg
    12781 caagtatttt taaaaaaagt taactcaaat ttatatttaa ttgactttga aaaagtattt
    12841 tattatgatt gagtaacgag gaaatggagc tgacatctcc agctgtgccg cagccgtcga
    12901 agccggaagg ggtaaatgtg gaagggcttt atgtctttac acaatactca actcgtaaaa
    12961 tataaaccca aacttcgagc taaagtttgt gtggaaaaga ttagtctttt tttactgaat
    13021 tctcgaagtg gtaaaaaagg acttttttaa tgcaattacc actttcatca ttgaataaac
    13081 caaaaattgg aacgtgtccg cacggacttc cgcagggcgc atgcccgatt tgctcgggca
    13141 tgggaggtgg cggcgcaaaa cgcgaggtta agtcaaaacc cgatgaaatg acttgggatg
    13201 aatgttttgc cattggtcaa atgcttaaag cacaaaaact cgctcaacag caaaaacagc
    13261 aagcggtaga gtcgcaactt caagtccccc ttaatttcgg ggcaaaattg gcaaattttt
    13321 cggcaaaaat tgcggaattt tcgcaaaagg ttgccaattt tgcccaaaag gcacaaggtt
    13381 taccaagtgt tatttcaaaa cccgttgttt ttgttttaaa caaattagtt attccgattt
    13441 tgaattttat caaagacgtt cctgttttaa tcaataaagc cgttaatttt gttaaagaaa
    13501 agctggcaga tatatcagat aaattaaatg c
//
LOCUS       DAOQAU010000043        13411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5065_length_13411_cov_5.859239, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000043 DAOQAU010000000
VERSION     DAOQAU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_13411_cov_5.859239"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..1182
                     /locus_tag="PKI94_06670"
     CDS             364..1182
                     /locus_tag="PKI94_06670"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW26456.1"
                     /translation="MEEIVKSLKELGFNSYEAKVYLALLKKYPATGYEVSQLASVPQA
                     RAYDTLKALEKEKVVVATNTKPVTYTPIKPEELTKRYKRKIISNIEFLEKNLPNVKED
                     YTEPILSVSGTASIREKIIEIVKNAQKEIYIEIWSQDFKFIEPYLWDAYNRGVEVKMV
                     GYDNFKSNFGLVFEHSFAKEIESSLGGRMIILAVDNNEGMIGNSSSTKNDLVHVVWTK
                     NPGVVFLIKEFIVHDMYLIDVEENLSEQMKIVYGKNMKKLRDKVLGSDYLYRIH"
     gene            1304..1933
                     /locus_tag="PKI94_06675"
     CDS             1304..1933
                     /locus_tag="PKI94_06675"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNW26457.1"
                     /translation="MQGYSFELITGPMSCGKTEELLRRVRRSIIAKKKVKVISPDIDT
                     RAHGDYIESRNGLWLEAIKVKHSIQILSVVKEDDEVVAIDELQFFDKNIAKVITQLME
                     SGKKVIGTGLDLDFKAEPFGYMPELLCIANRVDKLYAICMKCGSEYGTRTQRLIDGKP
                     ADKNSPLIMIGGDETYEARCQKCYELRDSQQEKEKNIKRSEFKLLPFTK"
     gene            complement(1986..3326)
                     /gene="amrB"
                     /locus_tag="PKI94_06680"
     CDS             complement(1986..3326)
                     /gene="amrB"
                     /locus_tag="PKI94_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNW26458.1"
                     /translation="MEKIKQPSVAGKFYTNNKNELVSQLNQFKSNCMGDYKYQSRAVI
                     VPHAGYVYSGQLAYEGISCISKTAKNIFIFAPSHYVGFDGLALSSFDKWLTPLGEISV
                     NQEINKEIKEKFSAEFFDEAFVSEHSAEVQVPFIQTLLNDVNIVPILIGRENCEKVSE
                     IVEYFWQKPDNAFVFSSDLSHFLNIKDAQKIDNLTAEMIETKDIEQFNSSQACGAVGI
                     CALVRFAQRNGFSLVRIGLTNSGEVTGDTSSVVGYGSWMLYEGEKNSFIKEFFSPIIK
                     DICAKSILARLKKQKEVRFKYPKVLDEKGACFVTLEKGDNLRGCIGSIIAHKPLIEDL
                     ISNAQNAAFSDPRFNPLREDEFDNLSIAVSLLSTPEKMFFKNENDLLSQIKPFVDGII
                     IKDGYHQAVYLPSVWEQLPEKSDFLNSLKVKAGLSANYFSNKFEAYRFYSEYIK"
     gene            3372..4370
                     /gene="amrS"
                     /locus_tag="PKI94_06685"
     CDS             3372..4370
                     /gene="amrS"
                     /locus_tag="PKI94_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNW26459.1"
                     /translation="MKYQNTLSDNITQCTICPRNCRLKEGQSGFCHIRKNVNGEIVLT
                     SYGYNTGLAIDPIEKKPLYHFYPGSLVLSFGTLGCNMGCDFCQNWRTTKSKDDSASLN
                     YTSPEKIADLAQEYGCKSVAFTYNDPVIFFEYAVDTAKICREKGIKTVAVTAGYINPE
                     PRKEFFKYMDAANIDLKAFNPEFYKKHCLANIEPILDTIKYVKNETDCWLELTTLLIE
                     GENDSEQDLQKECDWILKNLGDEVPIHFSAFHPDYRVTDKSKTKFSTLLKAYSIAKKA
                     GLKNIYTGNTCDIETATTYCSKCKKALIKRDCYHIEEYHLEKSTCAFCGAKCAGVF"
     gene            complement(4374..4811)
                     /locus_tag="PKI94_06690"
     CDS             complement(4374..4811)
                     /locus_tag="PKI94_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNW26460.1"
                     /translation="MKLTSVFKNGERIPAEYTCDGDDICPEFYVTDVPKTAKSLVMIA
                     DDPDAPVGLFVHWLLYNIPTSTDKFSAQDLPNGAVEGTTDFGRIGYGGPCPPNGTHRY
                     FFKLYALDKTLDLPSGVTKRKLEEEIREHIIEKAELMGVYSRH"
     gene            5155..5388
                     /locus_tag="PKI94_06695"
     CDS             5155..5388
                     /locus_tag="PKI94_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26461.1"
                     /translation="MSKGSGMGSKRDNSRFNKGLGRCTGKHSEQCGLDFEPKDKHTHD
                     KKHKHSFMDMDLPKKEEKHIYKKSYKGWDEEAL"
     gene            5476..5841
                     /locus_tag="PKI94_06700"
     CDS             5476..5841
                     /locus_tag="PKI94_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26462.1"
                     /translation="MSINSVNPVFLKFSSVQEQSSAKSVEQPEVKEQKPAKKASILDK
                     FETGIKDADYLNDKIQLPRTTFQGYLAFMTSAALTTLASIFKGKTRIGLNILSASTLM
                     IGSFYFVRPYIFKKDAPTN"
     gene            complement(5857..6210)
                     /locus_tag="PKI94_06705"
     CDS             complement(5857..6210)
                     /locus_tag="PKI94_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26463.1"
                     /translation="MTQITGISSNLALSQVESQSVSKSTQTVAAEEFAKVAESSIYVF
                     LVSSLKLPEYESGISKSDLTATLKTMIADHSEEQDNSNQIDLLTNVIADFKSLSAGND
                     FITEESLTSSQGFKA"
     gene            complement(6307..6840)
                     /locus_tag="PKI94_06710"
     CDS             complement(6307..6840)
                     /locus_tag="PKI94_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YD repeat-containing protein"
                     /protein_id="HNW26464.1"
                     /translation="MKKLLLFLIVFGSLCVQCFADNQGSVVYDGNKRLYYDDNGNLQG
                     STVYDGNKILNYDADGNLQSSTVRDGNKITNYDGQGNVKSSIVYDGNKVLKYDDKGKL
                     QGAKVRDGNTFTDYDPQGNVKNSTVQSGNKVTNYDGQGKIQGWTIYEGNTTKIYDAQG
                     TLINSTTRQGNSVQYKK"
     gene            complement(7343..7418)
                     /locus_tag="PKI94_06715"
     tRNA            complement(7343..7418)
                     /locus_tag="PKI94_06715"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7383..7385),aa:His,seq:gtg)
     assembly_gap    7537..7636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7787..8536)
                     /locus_tag="PKI94_06720"
     CDS             complement(7787..8536)
                     /locus_tag="PKI94_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW26465.1"
                     /translation="MTENAEMQSETDARQKVLVADDEASIRRILETRLKMVGYDVVVA
                     EDGEEAVNVFNKTNPDLVVLDVMMPKMDGYGVTREIRRTSDVPIIILTALGDVSERIT
                     GLELGADDYVIKPFSPKELEARVKAVLRRTSSREAVVPTGKITKNVITTGNIKIDTAR
                     RQVFRKNERIRLTGMEFSLLELLVNNSGQAYSRNEILQHVWAYPPDHRIDTRVVDVHI
                     SRLRSKLETDPANPELILTARGIGYMFQRIN"
     gene            complement(8728..11178)
                     /gene="recG"
                     /locus_tag="PKI94_06725"
     CDS             complement(8728..11178)
                     /gene="recG"
                     /locus_tag="PKI94_06725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNW26466.1"
                     /translation="MNVSREVRVGEDRVKGKLFENLEQIKQAIDIEIKHQYINVRGKN
                     KTFAKFIKGEIFSEYKKSKNHPKWKILMETFDQYSTDSFNVRKKAIKRLINVIKAELE
                     PPKAATKSAEEIKNPADVDVIYVKGVGPKVGYAMHKLGIYTAADLLFYFPRRHIDYSN
                     RTLIKNLKQGTNTTIFGYIKSVQAFVTKNNLSVLKVKINDESASLELNFFYAKTNRRL
                     LERYKSQFPVGAGFMASGTVKINSFDGRPTLDKPQYSIMSGDFLEQGNSNLNLGRIVP
                     IYPLNEGISIKTLRRAIFNAIETYKPVIKNVLPDFLREKYNIMNKADAVEQMHFPKSM
                     EELEQARFSLVFEELFLIQLKLAYLREENRKNNTAVPLGVNDKGLTKKFVESLPFELT
                     SAQKHAINDILKDLNSKEPMQRLLQGDVGSGKTVVATIMLLAAVENGYQGAIMAPTEI
                     LAQQHYNNLVEWLTPMGLSVGMFLGSNSKKIRAKIEKDLRNSQTHIAVGTHALIQENI
                     EFANLGAIVIDEQHRFGVKQRMLLKNKAQSPQMLTMTATPIPRTLALTVHGDLDLTVI
                     DELPKGRKPIKTALISSAAGQKEAYRVIQREVEAGRQAYVVYPLIDESETLSAKAATI
                     EAEKFQNEIFPQYKIGLLHGKLKNNEKEKVMADFKKKKYDILVSTTVVEVGVDVPNAT
                     VMLIENAERFGLSQLHQLRGRVGRSDLQSYCILSTSSRSAETLDRLKILTQTNDGFVI
                     AEQDLLIRGPGEFLGTRQSGLPDLIVADLIRDAKILELARGEAINFVKCYKLDKISAF
                     RDSLELNLMNDFDLINAG"
     gene            complement(11225..11758)
                     /locus_tag="PKI94_06730"
     CDS             complement(11225..11758)
                     /locus_tag="PKI94_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNW26467.1"
                     /translation="MTNNLIPVKVIENKIFVIRGIKVMLDSDLAELYQVETKILNRAV
                     KRNIKRFPDDFMFQLTNEEWEAVKVRCQFGTSPKGGGRQYLPYAFTEQGIAMLSGLLN
                     SDAAIFANIQIMRVFVKIKQYALENEEIFERLSAVEQYLMQYSKDNNIEIDKINKAIN
                     LLLDRTKPSQIGFNTKE"
     gene            complement(11796..>13411)
                     /locus_tag="PKI94_06735"
     CDS             complement(11796..>13411)
                     /locus_tag="PKI94_06735"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNW26468.1"
                     /translation="NSIKTIKNLVVGFVALNSPYENKTDGIRYDEKFKNKFYVEVNDK
                     RKNIYPSFYRSLSKYPAQYFNTVNKSGSIVTTNDVDGYIRVVDQFIDYKGTLYPSLAL
                     RMYDYLEQGDEKFIVKDKKITLEGSKLNIPVFSNYGGIFNNIKYYKLRPNSQYSHKTF
                     SAIDIIQSYDALKQGKTPTVSPQNFEDKIVFLGANAKAMAIGLADKKRTPMLEDYPGV
                     DIQATNLDNIMHNEFLVQTTLFQDIITILTLLAASFLIVMNFSLFASISINALLMLAY
                     ITACVFCYRFGLVISVITPIVLQVITMTVAYSYRFVLEGQNKEKIKKAMGKYISNDVM
                     QNVVKNIDTLSLGGKRANVTVLFADIRGFTSMSEKLSAEEVSLILNEYFTEIEPIISK
                     YNGVINKFIGDAVMAIFGEPIQDKNHALNAVKCADEMLKKVCELQKKWLAEGKPKIEI
                     GVGINTGEAFVGNIGSETRLEYTVIGDMVNLASRIESYNKTYKTNFLISSTTYEKVRN
                     IVDVIKISEVTIRGKAKKMDIYEVLRLVI"
BASE COUNT         4167 a         2456 c         2266 g         4422 t
ORIGIN      
        1 ataatattat ccgctcttct tgctttattt atcatttttc atctccgaat ataaaaactt
       61 gctcttattg tatcatattg ttgaaaaatt taaaacacca ttcttattat taacatttgt
      121 aacaaaattt tataaaaagt aagaattatt gttataaaac ttcaaaaagg tggtaattca
      181 tgcatgtaag ctcttttggg cttgcactct ctaattcctc tcttctaata cgtggttttt
      241 agccgacttg aaaaagtcgg tttatttttt gctttatttt taagtttttt gggcggcgga
      301 gaatttttta aattctccgc cgccccgttt gcaaagagat aaaaaatttg ttactattta
      361 aaaatggaag aaattgtaaa aagtcttaaa gaacttggat ttaactctta tgaagcaaaa
      421 gtttatctcg cgcttttaaa aaaatatcct gccacaggct atgaagtaag ccagcttgca
      481 agtgttccac aggcaagagc ttatgacacg ctcaaggcac ttgaaaaaga aaaagtcgtt
      541 gttgcgacaa acacaaagcc cgtaacttat actccgatta agcccgaaga gcttactaaa
      601 aggtataaaa gaaaaattat ttcaaacatt gagtttttgg aaaaaaatct gcccaatgtt
      661 aaagaagatt atacagaacc gattttgagc gtttctggca ctgcaagtat cagggaaaaa
      721 attattgaaa tagtcaaaaa cgctcaaaaa gaaatttata tcgaaatttg gtcgcaggat
      781 tttaaattca ttgaacctta cctttgggat gcttacaaca ggggtgtaga agttaaaatg
      841 gtcggttatg acaattttaa aagtaatttc ggacttgttt ttgagcactc atttgccaaa
      901 gaaattgaat cttctttagg cggcagaatg attattcttg ccgttgacaa caatgaaggc
      961 atgataggta attccagttc tactaaaaat gaccttgttc atgtggtttg gacaaaaaat
     1021 cccggggtcg ttttcttgat aaaagaattt attgtacatg atatgtattt aatcgatgtt
     1081 gaagaaaatc tttccgagca gatgaaaatt gtttatggaa aaaatatgaa aaaactcagg
     1141 gataaagttt taggcagtga ttatctttat cgaattcatt aataaaatgt tacttttcaa
     1201 atttttttta tgatttacaa tataaataca ggagttgaag gataaaaagt taaattgaca
     1261 tgtataacat ggctagagta tagggaaaca ggagtttatt aagatgcaag ggtatagttt
     1321 tgaattaatc acaggaccga tgagttgcgg taaaacagaa gaattgctca gacgagtaag
     1381 gcgcagtatt atcgctaaaa agaaggtcaa agtgatttct ccagacattg atactagagc
     1441 tcatggcgac tacattgaat ctagaaacgg actttggctt gaagcaataa aagtaaaaca
     1501 ttcaattcaa attcttagtg ttgttaaaga agatgatgaa gttgttgcaa tagatgagtt
     1561 gcaatttttt gacaaaaata ttgcaaaagt catcactcag cttatggaat caggcaaaaa
     1621 agttatcggt acgggactcg atttagattt caaagcagaa ccttttggtt atatgccaga
     1681 acttttatgt atagcaaaca gggttgacaa actttatgct atttgtatga agtgcggctc
     1741 tgaatacggc acaagaaccc aaaggcttat tgatggaaag cctgccgata aaaactctcc
     1801 tttgattatg attggcggag acgaaactta tgaagcacgt tgccaaaaat gctatgaact
     1861 gcgcgactcc cagcaggaaa aagaaaaaaa tattaaaaga agtgaattta aactgcttcc
     1921 attcacaaaa taaaaaattt aaaagtaaaa aaacaaggcg gctgcaaagc agccgccttg
     1981 tttttttact ttatatattc actgtaaaac ctgtaggctt caaatttatt tgaaaaataa
     2041 ttggcagaaa gccctgcctt tacctttaag gaatttaaaa aatctgattt ctccggcaac
     2101 tgttcccaca cagaaggcag ataaaccgcc tgatgataac cgtcttttat tattatgccg
     2161 tcaacaaaag gttttatttg acttagcaaa tcattttcat ttttaaaaaa cattttttca
     2221 ggtgtggaaa gcagtgaaac tgcaatagat aaattatcaa actcatcttc tctcagcgga
     2281 ttaaatctgg gatctgaaaa agccgcattt tgagcgttgc tgattaaatc ttcaatcaaa
     2341 ggcttatggg caataattga gccgatgcaa cctcttaaat tatccccttt ttccagggtt
     2401 acaaaacaag ctcctttttc atccaaaact ttagggtatt taaagcggac ttctttttgt
     2461 tttttaagcc ttgctaaaat gctttttgca caaatatctt taataatggg cgaaaagaat
     2521 tcttttataa aagaattctt ttcgccctca tacaacatcc agcttccgta accgacaaca
     2581 cttgaagtat cgcctgttac ttcgcccgaa ttagtaagcc ctattctgac aagagaaaat
     2641 ccgtttctct gagcaaatct gaccaacgca catattccga cagcaccgca tgcctgactt
     2701 gaattaaact gttcaatatc ttttgtttca atcatttcag cagttaagtt atcgattttt
     2761 tgagcatctt taatattcaa aaaatggctc aagtcagagg aaaacacaaa agcattatca
     2821 ggcttttgcc aaaaatattc tacgatttcg ctgacttttt cgcagttttc cctgcctatc
     2881 aaaatcggaa caatatttac atcattcagc aaagtttgaa taaacggaac ttgaacttca
     2941 gctgaatgtt cgcttacaaa agcttcgtca aagaactctg cgctgaattt ttcttttatt
     3001 tctttgttaa tttcctgatt aacacttatt tcgccaagcg gagtaagcca tttatcaaag
     3061 cttgaaagtg caagaccgtc aaagccgaca taatgtgaag gcgcaaaaat aaaaatattt
     3121 tttgcggttt ttgaaataca gcttatgcct tcataagcaa gctgtccgga atagacgtag
     3181 ccggcatggg gaacaattac cgctctggat tgatatttgt aatcacccat acaattcgac
     3241 ttgaattgat taagctgaga tacaagctca tttttgttat ttgtataaaa ttttcctgcc
     3301 acagaaggct gttttatttt ttccataact tacctattta atttttcaca agttggatta
     3361 taatataaaa aatgaaatat caaaataccc taagcgacaa tataacacaa tgtacgattt
     3421 gccctagaaa ttgcaggcta aaagaagggc aaagcggatt ttgtcatatc agaaaaaatg
     3481 ttaacggaga aattgtatta acatcttacg gatataacac gggcttagcc attgatccga
     3541 ttgaaaaaaa gccgctttat catttttatc cggggagttt ggttttatca ttcgggactt
     3601 taggctgtaa tatgggctgc gatttctgcc agaattggag gacaacaaaa tcaaaagatg
     3661 attctgcttc acttaactac acttctcccg aaaaaatcgc ggatttggct caagaatacg
     3721 gatgtaaaag cgtcgcattt acgtacaatg atcctgtaat tttctttgaa tatgctgttg
     3781 atactgcaaa aatatgtaga gaaaaaggaa ttaaaacagt tgctgtaaca gccggataca
     3841 ttaatccaga gccaagaaaa gagtttttta aatatatgga tgcagcaaac attgatttaa
     3901 aagcttttaa tccggaattt tacaaaaagc attgtcttgc aaacattgaa cccattttgg
     3961 atacaataaa atatgtcaaa aacgaaacag actgttggct tgaactcact acacttttaa
     4021 ttgaaggcga aaatgactct gaacaagatt tacaaaaaga atgtgattgg attttaaaaa
     4081 atttgggtga tgaagttcct atacatttta gtgctttcca tcccgattac agagttaccg
     4141 ataaatctaa aaccaaattt tcaacacttt taaaagccta ttcaatagca aaaaaggcag
     4201 ggcttaaaaa catatacaca ggaaacacct gtgatatcga aactgcaaca acatattgtt
     4261 caaaatgcaa aaaagcttta ataaaaagag attgttatca tattgaagaa tatcatttag
     4321 aaaaaagcac ctgcgcattt tgcggtgcaa aatgcgcagg cgttttctaa atcttaatgg
     4381 cgggaataaa cgcccattaa ctctgctttt tcaataatat gctcgcggat ttcttcttca
     4441 agttttcttt ttgtaacccc tgaaggtaaa tcaagcgtct tatcaagcgc ataaagctta
     4501 aagaaatatc tgtgcgtgcc gtttggagga cacggaccgc cgtaacctat tctgccaaaa
     4561 tcggtagtac cttcaaccgc tccgtttgga aggtcttggg ctgaaaattt atccgtagac
     4621 gtcggtatat tgtacaaaag ccaatgaaca aacaaaccca ccggcgcatc cgggtcatct
     4681 gctatcataa caagactttt agcagtttta ggaacgtccg tcacataaaa ttcaggacaa
     4741 atatcatccc cgtcacaggt atattcggcg ggaattcttt caccgttttt aaaaacactt
     4801 gttaatttca tattaccctc cttgccattg gcaaaatctt cggatttatt aaaccttaac
     4861 cctattatca acaataaaac aaatataaaa cctattatta aaaaacgaat tttcataatt
     4921 ttttataaaa ataactcaac ccaaaaataa aatcaagggg ggggtaaatg catattaacc
     4981 gcttgtgcaa cccaaaaata aaatcaaggg ggcaagatag taaatagtaa atggtaaatg
     5041 gtgaatagag ttgaataatt gaagattttt tgtggaaaat aacccttttg gggatagaaa
     5101 caaagtgcaa aatgctgaaa acttaaaaaa gcattttaaa taggagataa atttatgtca
     5161 aaaggaagcg gaatgggttc aaaaagagat aattcaaggt ttaacaaagg acttggaaga
     5221 tgcacaggaa aacacagtga acaatgcgga ttggattttg agccaaaaga caaacacacg
     5281 cacgataaaa aacacaaaca cagctttatg gatatggatt tgcctaaaaa agaggagaaa
     5341 cacatataca aaaagagtta taaaggctgg gatgaagaag ctttgtaaaa aaccgtaaca
     5401 ataaaaggtg tttagggttt ataaatttca ctttatagtg tatattagat aggctagcgg
     5461 tgaggtaaaa aagttatgag cataaatagt gttaaccctg tttttctaaa gttttcaagt
     5521 gtacaggaac aaagcagtgc aaaaagtgtc gagcagcctg aagttaaaga acagaagcct
     5581 gcaaaaaaag cttctatttt ggataagttt gaaacaggaa ttaaagatgc cgattatctg
     5641 aatgacaaaa tacagcttcc cagaacgact tttcaggggt atcttgcttt tatgacaagt
     5701 gccgctttaa cgactttggc gtcaattttt aaaggaaaaa caagaatagg gttaaatatt
     5761 ttatctgcaa gcacgctaat gataggctct ttttattttg tcagacctta tatatttaaa
     5821 aaagatgccc cgacaaatta agttttaatc aagaaattaa gctttaaatc cttgacttga
     5881 agtcaaagat tcttcggtga taaagtcatt gccggcagaa aggcttttaa aatctgcaat
     5941 tacatttgtc agcaaatcga tttgattgct gttgtcttgt tcttcactgt ggtctgcaat
     6001 cattgtcttg agtgttgcag tcaaatccga tttacttatc ccgctttcat actcagggag
     6061 tttaagtgaa cttacaagaa agacataaat gctcgattct gcgacctttg caaattcttc
     6121 cgcagcaaca gtttgagtgc ttttacttac gctttggctc tcgacttggc ttaatgctaa
     6181 atttgaacta atccctgtta tttgtgtcat gacaaaaatc cgtttttgaa aacaaacccg
     6241 atattttaat tatatttttg tttaaaaata tgtcaaaatc ttaataaaat ttaatttttg
     6301 gggcgattat tttttatatt gaacagagtt accctggcgg gttgttgaat ttataagagt
     6361 gccttgcgca tcataaattt ttgttgtatt tccttcataa attgtccaac cttggatttt
     6421 gccctgtccg tcgtaatttg taaccttatt gccgctttgc accgtggaat ttttgacatt
     6481 gccttgaggg tcataatcag taaaagtgtt gccgtctcta acctttgccc cttgaagttt
     6541 gcctttgtca tcgtatttta aaaccttgtt tccgtcataa acgattgaac ttttaacatt
     6601 accttgcccg tcatagttag tgattttatt tccgtctcgt acggtagaac tttgaagatt
     6661 accgtctgca tcgtaattta aaattttatt tccgtcatat acggtagagc cctgaaggtt
     6721 gccattatcg tcgtaataaa gtcttttgtt accgtcataa actacagaac cttgattatc
     6781 agcaaaacat tgaacgcaaa gcgagccaaa aacgattaaa aagagtaata attttttcat
     6841 attttctaca ccttaactga aaaaatccga ttatgcttct aatttcatta taaacacaat
     6901 tgaatttctt gtcgaggatt taataaataa agtggggaca atgcctaccg gctttattga
     6961 agttgtcatt gcgagggggt aaatgattta gcttattttt gaagtctttt gcgtcattgc
     7021 gaggagcaac gcgacgaagc aatccaaaaa accgcgtggg caagtatgcc caccttactt
     7081 gttgaacccg ttgacataaa taaaaaggac tatctttcga tagtcctttt tatttatggg
     7141 gcgagtgatg gggttgtctt ggattattgg cagttcgcaa aagcgtggtt ttgctcacaa
     7201 ggacgtgctg gggtcgtttc actcccacgg cgcacttgcc aaaatccgct cgaacccaaa
     7261 caaaacttcg ttttgtttgg gttctcacct aatacttaca cctaattaaa aagaacttcc
     7321 aaaatatgga agttcttttt aatggggcga gtgatggggt tcgaacccac gtatatcgga
     7381 accacaatcc gaggtcttaa ccacttgacg acactcgcca tatttacgtc attgcgagac
     7441 ttttatcttc gactgagcta aaagcttcca ccgaagcaat ctcattcata ttttactaca
     7501 aacaaagcgg ctgtcaaaga ttttgacagc cgctttnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnntcat tcatatttta ctacaaacaa agcggctgtc aaagattttg
     7681 acagccgctt ttatatttac ttaattctca caatgttaga ttttgagatc ctgaatcaag
     7741 ttcaggatga cgtatttgta aggttcgttc ctcaccgaca attacgtcaa tttattcttt
     7801 gaaacatata tcctatcccg cgtgcggtta ggattaattc agggtttgca gggtcagttt
     7861 ccaactttga acgcaatctt gaaatatgta catcaacaac tcttgtgtca attctgtgat
     7921 caggcggata tgcccaaacg tgctgtaata tctcgtttct tgaatatgct tgacctgaat
     7981 tgtttaccaa caattccaac aagctgaatt ccattcctgt aagacgaatt ctttcgtttt
     8041 ttctgaaaac ttgacgtctt gcagtatcaa ttttgatatt tcctgttgta atcacatttt
     8101 ttgtgatttt gcctgtagga acaacggctt cgcggctact tgttcttctc aatacagctt
     8161 taacccttgc ttccaattct tttgggctga aaggtttgat aacatagtca tctgcaccta
     8221 attcaagtcc tgtaattctt tcggaaacat ctcccaatgc ggttaaaata atgataggaa
     8281 catcagaagt tcttctgatt tctcttgtaa ccccgtatcc gtccattttt ggcatcatta
     8341 cgtccaatac cactaaatcg ggattagttt tgttaaatac atttacggct tcttcaccat
     8401 cttctgctac aacgacatcg tagccaacca ttttcaaacg tgtttctaaa attcttctga
     8461 tacttgcttc gtcatctgct accaaaactt tttgacgagc atctgtttca ctttgcattt
     8521 cagcattttc tgtcatagtc gtaatcctta aaatttatgt gctaccagtt cttaattatt
     8581 tacataaata aatattatta caaaatattt atttttttaa attttcttta ataaatataa
     8641 acttatctta atttaattaa caaaaaattg caagcacttt tttaaaaaaa atattatcaa
     8701 ttttataaaa cagtattaaa gcctttatca gccagcgttt atcaaatcaa aatcattcat
     8761 aagatttaac tctaaagaat ccctaaatgc tgatattttg tcaagcttgt aacattttac
     8821 aaaattaatc gcttctcctc tggcaagctc caaaattttg gcatctctga ttaaatcagc
     8881 tacaattaaa tcaggaagtc cgctttgtct tgttcctaaa aattcacccg gacctcttat
     8941 cagcaaatct tgttctgcaa ttacaaaacc gtcatttgtt tgagttaaaa ttttaagtct
     9001 gtcaagcgtt tcagccgacc ttgaggatgt cgaaagtatg caatatgact gtaggtcgct
     9061 tcttccgacc ctgcctctga gctgatgcaa ctgtgaaagc ccgaatcttt ctgcgttttc
     9121 aataagcatt accgttgcgt ttggcacatc aacacctacc tcaacaacgg ttgtagagac
     9181 caaaatgtca tattttttct tcttaaaatc tgccataact ttttcttttt cattgttttt
     9241 gagttttccg tgtaaaagtc cgattttgta ttgcggaaaa atttcattct gaaatttttc
     9301 cgcttcaatc gtcgccgcct tagcagaaag cgtttcactc tcatcaatca aggggtaaac
     9361 cacatatgcc tgtcttccgg cttcaacttc tctttgaata actctatatg cttctttttg
     9421 ccccgccgca gagcttataa gtgctgtttt aataggcttt ctgcctttgg gaagctcatc
     9481 aataaccgtc aaatctaaat caccatgaac ggtaagggca agtgttcttg gaatgggcgt
     9541 tgcagtcatt gtaagcattt gcggactttg cgctttattt ttaagcaaca tcctttgctt
     9601 aaccccaaat ctgtgctgtt cgtcgattac aattgccccc aaattagcaa attcaatgtt
     9661 ttcctgaata agtgcgtgag ttcccactgc gatatgggtt tgggaatttc tcaaatcttt
     9721 ttcgattttt gcacggattt tttttgaatt gcttcccaaa aacattccca cagatagtcc
     9781 catcggggta agccattcca caagattgtt gtaatgctgc tgtgctaaaa tctcggtcgg
     9841 tgccataatc gcaccttggt agccgttttc taccgcggca agaagcatta tcgtagcaac
     9901 aactgttttt ccgctgccca catcgccctg tagaagtctt tgcataggct ctttggaatt
     9961 caaatccttc aaaatgtcgt taattgcgtg tttttgagca cttgtcaatt caaacggaag
    10021 gctctcgaca aactttttgg ttaagccttt atcatttacg ccaagaggta cggcggtgtt
    10081 gtttttgcga ttttcttccc taaggtatgc aagcttaagc tggattaaaa acagctcttc
    10141 aaagaccagc gaaaaccttg cctgttcaag ttcttccata gatttgggga aatgcatctg
    10201 ctcaacggca tctgctttgt tcataatatt atatttttct cttaagaaat cgggtaaaac
    10261 gttttttatc acaggtttat aagtttcaat cgcgttaaaa atcgcccgcc ttaaagtttt
    10321 tatactaatc ccctcgttaa gcgggtaaat gggtacaatt cgtcccaaat tcagattaga
    10381 attgccttgt tcaagaaaat ctccgctcat aatagaatat tggggtttgt caagagtggg
    10441 tcttccatca aaagaattaa ttttaaccgt tcccgaggcc ataaagcctg ctccgaccgg
    10501 aaactgtgat ttataccttt caagcagtct gcggttggtt tttgcataaa agaagttaag
    10561 ttccaggctt gcgctttcgt cattaatttt gactttcaaa acgctcaaat tattttttgt
    10621 cacaaatgcc tgaactgatt taatgtagcc aaaaattgtt gtattagtgc cttgcttcag
    10681 atttttaatc aaagttctgt ttgaataatc aatatgtctg cgcgggaagt aaaaaagcaa
    10741 atccgcagcg gtataaatac ccaatttatg catagcgtag ccgactttcg gaccgacacc
    10801 ttttacataa atcacatcga catctgcagg gtttttaatt tcttcagcac tttttgttgc
    10861 ggcttttggc ggctctaatt cagctttaat aacatttata agccttttta tcgctttttt
    10921 tcttacatta aaagagtctg tggaatactg gtcaaaagtt tccattaaaa tcttccattt
    10981 cgggtggttt ttagattttt tgtattcgct gaaaatttcg cctttgataa attttgcaaa
    11041 ggttttgttt ttgcccctga cattgatata ttgatgctta atttcaatat caatagcttg
    11101 ttttatctgc tctaaatttt caaacaattt acccttaacc ctgtcttcgc caacccttac
    11161 ctctctcgaa acattcaccg aatgtttcgt tcggcagagt ctcaaggggc gaggtaataa
    11221 tatattattc ctttgtgtta aaccctattt gagacggctt tgttctgtct agtaataaat
    11281 ttattgcttt attgatttta tctatttcaa tattgttatc ttttgaatat tgcattaaat
    11341 actgttcaac cgccgatagt ctttcaaaaa tttcttcgtt ttcaagtgca tattgtttaa
    11401 tttttacaaa tacgcgcatt atttgtatat tggcaaatat tgcagcatcg ctgttcaaaa
    11461 gtccagataa cattgctata ccttgttctg tgaaagcata aggcaaatat tgtcgtccac
    11521 cgccttttgg tgaggtgcca aattggcacc tcaccttaac ggcttcccat tcttcatttg
    11581 tcagctgaaa cataaaatca tccggaaatc gttttatatt acgtttaacc gctctattta
    11641 agatttttgt ttctacctga tataattctg ccaaatcact atctaacatt actttaatac
    11701 cacggattac aaaaatttta ttttcaatta ccttaacagg aattaaatta ttagtcatcg
    11761 tttacctcaa aataatttta acataaaaaa tacacctata taaccagtct taagacctca
    11821 taaatatcca ttttcttggc ttttcccctg attgtaacct ctgaaatctt tatgacatca
    11881 acaatatttc ttactttttc gtaagtcgta gaactgatta aaaaatttgt tttataagtt
    11941 ttgttataac tttcaattct gcttgctaaa tttaccatat cgccgataac tgtgtattca
    12001 aggcgggtct cagagccgat atttcctaca aaagcttctc ctgtgttaat tcctacgcct
    12061 atttcaattt tcggtttgcc ttctgctaac cattttttct gcagttcaca aaccttttta
    12121 agcatttcgt ctgcgcattt tacggcattt agggcgtgat ttttgtcctg aatcggttct
    12181 ccgaaaattg ccataaccgc atctccgata aatttattta taaccccgtt gtatttgctt
    12241 ataatcggct caatttccgt aaaatattcg tttaaaataa gcgaaacttc ttctgcagaa
    12301 agcttttcgc tcattgaggt gaaccctctg atatctgcaa aaagcaccgt gacgtttgcc
    12361 ctttttccgc ccagagaaag tgtatcaata ttttttacga cattttgcat aacgtcatta
    12421 gatatgtatt tccccattgc ttttttgatt ttttctttgt tttgcccttc aagcacgaat
    12481 ctgtatgaat atgcaacagt catagtaatt acttgaagga caataggcgt aattacgctt
    12541 attacaagcc cgaatctata gcaaaaaacg caggcggtta tataagccaa cattaaaagg
    12601 gcatttatgc tgattgaagc aaaaagcgaa aagttcatta caattaaaaa cgaagcggcc
    12661 aaaagcgtta agattgttat aatgtcttga aataaagttg tttgtaccaa aaattcattg
    12721 tgcataatgt tgtctaaatt cgttgcttga atgtcaactc cgggatagtc ttcaagcata
    12781 ggcgttcttt ttttatccgc aagtcctata gccattgctt ttgcgtttgc acctaaaaaa
    12841 actattttat cttcgaaatt ttgaggagaa actgtcgggg ttttcccttg cttcaaagca
    12901 tcataagatt gaattatatc tattgccgaa aaagttttat gcgaatactg tgaatttggt
    12961 cttagcttgt aatatttgat attgttaaaa attccgccat aatttgaaaa tactggaata
    13021 ttgagttttg agccttcaag tgtaattttt ttgtctttta caataaattt ttcgtcacct
    13081 tgctctaaat aatcgtacat tctaagtgcc aaagaaggat acaaagttcc tttataatca
    13141 ataaattgat caacaactct tatataaccg tcaacatcgt ttgttgtaac aattgaaccg
    13201 gatttattta cggtgttaaa atattgcgcg ggatattttg acaaactgcg ataaaatgac
    13261 ggataaatat tttttctttt atcattgact tcaacataaa atttgttttt aaatttttca
    13321 tcatatctta tgccgtctgt tttattttca taaggggaat taagtgcaac aaaaccgacc
    13381 acaaggtttt ttatagtttt aattgaatta t
//
LOCUS       DAOQAU010000044        13380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5078_length_13380_cov_6.755047, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000044 DAOQAU010000000
VERSION     DAOQAU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5078_length_13380_cov_6.755047"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..653)
                     /locus_tag="PKI94_06740"
     CDS             complement(36..653)
                     /locus_tag="PKI94_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26469.1"
                     /translation="MAVGLDTEHNYLAQYIAKQNQVGSFTSGDKAKVTEVPKAEETKT
                     DTVEISKKDEPKADKKKYSFKTFIADVADAWINFKEITKGIFKGLLVGGVATTFGLFI
                     DKMSSGLKAAGKFKPAEKVAEAAAKKFTIWTAVASKFKNNKDILSCELSVFKKAFAKG
                     STKGKIVSGLIGAAILTGYIIAARLRANLKTADVDHALHEGHRDK"
     gene            complement(730..1590)
                     /locus_tag="PKI94_06745"
     CDS             complement(730..1590)
                     /locus_tag="PKI94_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNW26470.1"
                     /translation="MIAKNKLEELVKKYEVVDFIKNDPIQFSYRFFDVIARQELSALD
                     KSSTEAIFTDIEIKNIEIAAFLSSLFAYGNREVFIKKLDELFEIMKNQPLDFVLNFNL
                     SVLRGFNYRFAKDSDIIEVFRILNKLYSKGGDLKKLFEYGYSLPSPLGGEGRVRGKIY
                     SMLQAVVDYFYSNAKNEAGAGFYHLIPNPQNGGAMKRMNLFLRWMVRKPPVDLGIWDF
                     ISPSELLIPLDVHVARLSRQMGLLHRSSNDAKAVIELTQNLKKIDKNDPAKFDFAIFG
                     LGIDSKDKNT"
     gene            complement(1587..2021)
                     /locus_tag="PKI94_06750"
     CDS             complement(1587..2021)
                     /locus_tag="PKI94_06750"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNW26471.1"
                     /translation="MNSNDFMKQALGVAEKSGADVPVGALIVQNGTIISACHNEKEQN
                     KDSTAHAEICAIKKASLVLSRWRLDDCEMYVTLEPCPMCAWAIIQSRIKTVYFGSYDI
                     QYGALVSVLDLRKQAKSDLKVFGGILEKECDNIIKEFFGKIR"
     gene            complement(2018..3700)
                     /locus_tag="PKI94_06755"
     CDS             complement(2018..3700)
                     /locus_tag="PKI94_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW26472.1"
                     /translation="MKKLKLHRLKIAEQILVVLLFAVIIPMAISGFIINNINQQSIRS
                     QLRDSAMLIAKMVSQEVDIFITSSLNELNQIKFAADYMGGTYSKPKYFNKVLKNSPEF
                     QNLEILNTKQDLLKLHENNLKNNNVSIYTTMRDNKFLVATFNVDFVKKSLFKPVLEDE
                     RQIYVLSSNGTLIAAHNFKEKDYQNTMKYLPKKLKTDTPVIFGKTKNQPLVYYKKTSP
                     NIMIVVNTTKGVMKKTINANRFKIILAVLIAALTVIFVVGLYTYYLYINIRQLFKGII
                     AISKGNYQRRIRLLTNVFTPYEIVFLAFEFNRMASQIHKSYIQLKKKNVELKDLNEFR
                     SNLVDTVSHEFRTPLTSIQGYTSRLLRQDIEIDEDTKQKSLKTIKKQSERLSRMVEDL
                     LVVPDIEGSKLRIQIEPVWLSKTINSAITLVKSDEIKIVNKVSDDFPLVLADKDRFEQ
                     VLVNLLENAVKYSQEKKEVTVESKIFKKYAKIYVKNDCKVIPKDKLNVLFEKFTRIDD
                     KTTRTTRGTGLGLYIVKGLVEAMNGEIKLSSTKEKGFVVEIKLPIFNPEKVK"
     gene            complement(3734..4444)
                     /locus_tag="PKI94_06760"
     CDS             complement(3734..4444)
                     /locus_tag="PKI94_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW26473.1"
                     /translation="MKTQKRILIVDDDTEIRELLEFDIGQSGYYVDTAKDGLEGLNKA
                     LNNAYDLIILDVMMPKMNGFDVCKNIRQAKLAVPVLMLTAKGTIIDKTEGFDCGADDY
                     LVKPFDIQEVLLRIRVLLRRNQIEEDNSISSEVLRAGDIEIFPDTLECAVVNKRIKLT
                     PTEFEILYCLMQHFNSPVTLAVLLDEVWGYDSSEDVRMLRVHVGGLRNKIEPDGKNPK
                     YLHTVTNVGYKLTPFTEE"
     gene            4777..8694
                     /locus_tag="PKI94_06765"
     CDS             4777..8694
                     /locus_tag="PKI94_06765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNW26474.1"
                     /translation="MLIQGPISSEKTQLLTQKYAELLNSGVDSTKILVLLQNSNKKEK
                     FIKETLENLKIETVEKLQIYSFYGLVYNTITDNWAFLENSIKDSNTKILPNLVGLEVS
                     QSLLKDIIKDVKFEGYNSKKSLLHQLFRRYSLIVQNNLTDVDVKKRSEEILKESFAPD
                     AKAALDEFKKRTLELRSFDYLRQSLIFNHLYKNTGCFSKIEYLLVDDGDEITPICLDF
                     IKFLQPKLKDIFIAYDPLGSSRWGYLSADKSAVFGFEEMSNEAPLVLPAKTALKEDAQ
                     TIFNNATGQKNQKLINFSLFSTSKRAQMLDEGIKKVKSLINNSIKPCEIAIITPIIDD
                     MLKFSLRENLPKSQVNPVFLSGSEKIVQNPIVAYCLSILKLCDETLKQELCEFEIRAI
                     LSDFLKIPIKYCRKILDEFEKTKEFIPFTFKNEAFTKNYAAFLNLLENLKKTNKLSEQ
                     IYGISELIDFKFYDKDEINKFNFFIKQIEDFESVFTDKKISNSQIINQIENSIISENP
                     YSTLEIEENDLVIATPQKIIDNQIQTKYQIWLDVSSDEWIKSDTGPLYNAWVFQAGWD
                     KAEFTLQDNIELSRQKTARILRKLTLCAGEKVYTYASLFDSMGVENFGGIEGGVNIGG
                     VNEGVNAGGVNEGVAEAKVKKIIPRDDQKAVLDYKSGSMAISAVPGAGKTTILLSLIL
                     KLLEEGTNPENIFVLTYMESAARNFRERIQLANPYSTQLPNISTIHGLALKILKENSN
                     FEKLGLNPDFEICDDTQRSRILRELSAKLKLKKTESEDFDRAVSVLKISKGTNKIENK
                     KAELSKFLEFFNEYDQILKENNLIDYDDMLILSVKLLKENPDVLKYYQDICLYVIEDE
                     AQDSSQIQQELISLLSSKHKNLIRCGDVNQAITTTFSNADVEGFRRFINQAQTKVSMN
                     RSQRCTKEVWELANNLLKTSQKNPKTQNAFYEIFMRPVEGKNPQERNSIVPIVFDNEF
                     TEKNFILKQIKTLISKNPDKTLGILLRNNYQVTNWTKFINNSGLKTITRSECLEQKGI
                     FRTIFAVLKIIQNPFDNENIAQNYDILAELGHYTSGMFAQIRNFPSVFVQTSTDDIKN
                     LDLVDFYWDVDYWLNFSSMPIEELAIKIGLYYYPDSQIEKSNVYLIATLIKRLSVQYK
                     KFSAILDRLSELAKKPNLSGFKFFSEEDEDDKKFLEGKVQIMTMHKSKGDEFDFVFLP
                     EFSEDSLPIEFENMSLKKNSRFMEEVRELNPDYRKKTDDDLKEFLLAENLRLLYVAIT
                     RAKRKLFFTVSKITKPYGKEKEQKPSAIFEKLLKPRNSEVTDE"
     gene            8687..9343
                     /locus_tag="PKI94_06770"
     CDS             8687..9343
                     /locus_tag="PKI94_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNW26475.1"
                     /translation="MNNFSPNMLKNFDQCQQKFCFKYVEKISMPQKPEIFEKGKKIHA
                     LANYFLNKNDISKMEQTLTQAEKTDWEALKSNKYMSFEPVVTEHNLSCKIGDFWVGGR
                     LDAIVKQGNDYFILDYKTGNIPQNPQNDFQTMVYLLAADKIFKQKNIPVNSITFVYIG
                     LKQGEEKSILFNEDLKKTYEENLKEICKKIDFTCDAKVFSKTLTACKYCEYNKICYKD
                     "
     gene            9920..10084
                     /locus_tag="PKI94_06775"
     CDS             9920..10084
                     /locus_tag="PKI94_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26476.1"
                     /translation="MQCPKISLFERSEFEIFNGMSRTLGKSDEQGVAFGGTFCHQKVP
                     KENIQEIITF"
     gene            10264..10977
                     /locus_tag="PKI94_06780"
     CDS             10264..10977
                     /locus_tag="PKI94_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26477.1"
                     /translation="MTNAVASAIFCARNVDKTKHGEVGRSAVAVGQAKKVIDSIIQLD
                     KKLGKGPAKAVENILNFSNKSKALQAIGKITKFASDNINPLICVSSGIKVLTSDDKKT
                     TLINETGALSGMFISEKVAKKLLDNMVESKTVKELCEKGIKSADAKGYRTLSKLIKSS
                     PTILKGILFVFASIGGYSIGSKIAKKISSKEKKPAKNENIFAKAPLAEQKVQNKAPEK
                     IEKTVQKEEVEPKKVDVAA"
     gene            11049..11120
                     /locus_tag="PKI94_06785"
     tRNA            11049..11120
                     /locus_tag="PKI94_06785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11081..11083,aa:Thr,seq:tgt)
     gene            11194..11265
                     /locus_tag="PKI94_06790"
     tRNA            11194..11265
                     /locus_tag="PKI94_06790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11226..11228,aa:Thr,seq:ggt)
     gene            11720..13030
                     /locus_tag="PKI94_06795"
     CDS             11720..13030
                     /locus_tag="PKI94_06795"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW26478.1"
                     /translation="MTLDLSKNESVILEYKEEINTNFYKSLSAFANTKGGIILLGVTD
                     KKEPKGIDLPKGRQETIINQIVDSLGIQPEINILTIEGKNILSVLVEKSSNPISYKGK
                     YYKRVGNTTREMQREELKKMFLKDVSWDTLTGNYSIDEIDTEIVRYFIKLANSKKRLP
                     IVGEDESIQSILERLDLIIDGKLTNASILLFGKNPQKYFTSATLRVGAFKDNTGVTFI
                     GDRQIKGNLFKQAIDGEEALKFYNNVKYVISGKLQRDEYWDYPLDAVREALLNALIHR
                     DYFDSGTEIQAKIFKDSLWIYNSGGLTGGLTLEQIKGQHPSRSRNKLIMNIFYLSGMV
                     ERFGSGISRIIEKCKSQLLPEPEFKEESGGFSVYFRKEFSGLNDRQKKAIEFIREKGA
                     INNSLYQELTQATRETSKRDLFKMVEVGIIKKEGEGKLTKYILP"
BASE COUNT         4591 a         2341 c         2317 g         4131 t
ORIGIN      
        1 gaaaaatcgc cctcaatgtt tatttatttc ttaaattatt tatctctgtg accttcgtga
       61 agtgcgtggt caacatctgc cgttttcaag ttagcacgga gtcttgctgc gataatatag
      121 cctgtaagaa tagcagcacc gattagacct gaaacaattt tgcctttagt cgaacctttt
      181 gcaaatgctt ttttaaatac actcaattca caactaagaa tatctttatt atttttaaat
      241 ttacttgcaa cagctgtcca aatagtgaat tttttagctg cagcttcagc aactttttca
      301 gcaggtttaa attttcctgc cgcttttaat cctgaagaca ttttgtcaat gaacaaacca
      361 aatgttgtgg caacaccgcc tactaacaat cctttaaata taccttttgt aatttctttg
      421 aaattaatcc aggcatcagc gacatcagct ataaaagttt tgaaagaata ttttttctta
      481 tcggcttttg gctcatcttt tttagaaatc tcaacagtat ctgtctttgt ttcttcagcc
      541 ttaggaactt cagtgacttt ggctttgtca ccggaagtaa acgagccaac ctgattttgt
      601 tttgcgatat attgggctaa ataattatgt tctgtatcta aacctactgc catagcacac
      661 cccttttttt attattctaa cgttttaaac cccaaaaatg caaaaaattg ctagtttgtt
      721 aataaaagtt tacgtatttt tatccttact gtcaattccc agcccgaata ttgcaaaatc
      781 gaattttgca gggtcgtttt tgtctatttt tttcaaattt tgcgtaagct caattactgc
      841 ttttgcatca ttgctgcttc tatgcagaag tcccatttgc cttgaaagtc ttgcaacatg
      901 gacatcaagt ggaatcaaaa gttcgcttgg agaaataaaa tcccaaattc ccaaatccac
      961 aggtggtttt cgcaccatcc agcgcaaaaa taaattcatc cgcttcatcg caccgccgtt
     1021 ttgcgggttt ggaattaaat gataaaatcc cgcaccagct tcattttttg cgtttgaata
     1081 aaagtaatcg actactgcct gaagcattga ataaatttta cccctcaccc tgccctctcc
     1141 cccaaggggc gagggtaaag aataaccata ttcaaaaagt tttttcaagt caccgccttt
     1201 ggaataaagt ttgtttaaaa tcctaaaaac ttcgattata tccgaatctt ttgcaaagcg
     1261 gtaattaaat cccctcaaca cgctcagatt aaaattcaaa acaaaatcaa gcggctgatt
     1321 tttcattatt tcaaaaagtt cgtctaattt ttttataaaa acttctctgt tgccgtatgc
     1381 aaataaagat gataaaaatg cggctatttc gatgttctta atttcaatat ccgtgaagat
     1441 tgcttcggtg gaagatttat ccaaagccga taattcttgt ctcgcaataa cgtcaaagaa
     1501 acggtatgaa aattgaatcg ggtcattttt aataaaatca acaacctcat atttttttac
     1561 taattcttct aatttatttt ttgctatcat ctgattttgc caaaaaattc ttttataata
     1621 ttatcgcatt ctttttctaa tattccgcca aaaaccttca aatctgattt tgcctgtttt
     1681 ctcaagtcca aaacagaaac caatgccccg tattggatat cgtaagaacc gaaatagacc
     1741 gtttttattc ttgattgaat aatcgcccag gcacacatag gacagggttc aagtgtcaca
     1801 tacatctcac aatcatctaa ccgccacctg cttaaaacta aagaagcttt ttttatagca
     1861 caaatctcgg catgagcagt tgaatcctta ttctgctctt tctcattatg gcaggcggaa
     1921 attattgttc cgttttggac tataagggca ccgacaggta cgtcggcacc tgatttttct
     1981 gccaccccca aagcctgctt cataaaatca ttgctgttca ttttactttt tccggattaa
     2041 aaatcggcaa tttaatttcc acaacaaagc ctttttcttt tgtcgaagaa agtttaattt
     2101 caccgttcat agcctcaaca agccctttta ctatgtataa gcccaaccct gttcctcttg
     2161 tggttcttgt tgttttatcg tcaattcggg taaatttttc aaacagaaca ttcaatttgt
     2221 cttttggaat aactttgcaa tcgtttttga cataaatttt tgcgtatttt ttgaaaattt
     2281 tgctttcaac agtaacttct tttttctctt gggaatattt tactgcgttt tcaagaagat
     2341 taaccaaaac ctgctcaaac ctgtctttgt ctgcaagcac aagcggaaaa tcatcagaga
     2401 ctttgtttac aatttttatt tcatcgcttt ttacaagtgt gattgcagaa ttaattgttt
     2461 tgctgagcca aacgggctct atttgaatac gtaattttga gccttcaata tcgggtacaa
     2521 caagcagatc ttccaccatt ctggagagcc tttcggattg ttttttaata gtctttagcg
     2581 atttttgttt tgtatcttca tcaatttcaa tatcttgtct taaaagcctt gaggtgtagc
     2641 cctgaatgct tgtaagcggg gttctgaatt cgtggcttac ggtatcaacc aaatttgaac
     2701 gaaattcgtt taagtctttc agctcgacgt ttttcttttt cagttggatg taagatttat
     2761 ggatttggct tgccatgcgg ttaaattcaa acgcgagaaa aacaatctca taaggagtaa
     2821 aaacatttgt caaaagtctt attcgccttt gataattccc ttttgaaatt gctataatcc
     2881 ctttaaaaag ctgtctgata tttatataaa ggtaataagt ataaagcccc accacaaaaa
     2941 ttaccgtcag agcagctatt aaaaccgcaa gaattatctt aaatctgttg gcattaatcg
     3001 tttttttcat aacgcctttt gtcgtgttaa ccacaatcat tatgttgggg cttgtttttt
     3061 tgtaataaac aagcggctga tttttggttt taccaaagat tacgggagta tctgttttaa
     3121 gttttttggg aagatacttc attgtgtttt gataatcttt ttccttaaaa ttgtgtgctg
     3181 caataagcgt tccgtttgaa gacaaaacat aaatctgtct ttcgtcttcc aaaacaggtt
     3241 taaaaagact cttttttaca aaatcaacat tgaaggttgc aacaagaaat ttattatcgc
     3301 gcattgtcgt ataaattgaa acattgttat ttttcaggtt attttcgtga agtttaagca
     3361 aatcttgttt tgtattaaga atttccagat tttgaaattc gggagaattt ttcaaaactt
     3421 tattgaaata tttcggcttt gaataagttc cgcccatata gtctgcggca aattttatct
     3481 gattaagttc gttaagtgaa gaagttataa aaatatcaac ttcctgcgaa accattttcg
     3541 caattagcat agcagaatct cttaactgcg aacgaattga ctgctggtta atattattga
     3601 taataaaccc gcttattgcc ataggaatta ttactgcaaa gagcaataca accaatattt
     3661 gttctgcaat tttcaatctg tgaagtttta attttttcat cttctaatcc ttaatccgac
     3721 ttctaattta accttattct tcggtaaaag gggttaattt gtaacccaca tttgtaactg
     3781 tatgcaaata cttggggttt ttgccgtcgg gctcgatttt gttacgaagc ccgcccacgt
     3841 gcactctcaa catccttaca tcttcgctgc tgtcgtagcc ccagacttca tcgagcaaga
     3901 ccgccaatgt gacaggcgaa ttaaaatgct gcataagaca ataaagtatt tcaaactctg
     3961 tgggagtaag ctttattctt ttattgacaa cagcacactc aagcgtgtcg gggaaaattt
     4021 caatgtcgcc tgcacgcagg acttcggatg atattgagtt gtcctcttca atttggttcc
     4081 ttcgaagcaa tacccttatg cgcaaaagca cttcctgaat atcaaaaggc ttgaccagat
     4141 aatcatctgc cccgcagtca aaaccttcgg ttttgtcaat aattgtacct ttggctgtga
     4201 gcatcaaaac cggtacggca agctttgcct gcctgatatt tttacaaaca tcaaatccgt
     4261 tcattttggg catcataaca tcaagaatta tcaaatcgta agcgttattt aacgccttat
     4321 taagcccttc aagcccgtct tttgcagtat caacatagta accgctttga cctatgtcaa
     4381 attcaagcag ttccctgatt tcggtatcat catcaacgat taatattctt ttttgagtct
     4441 tcatcgaaaa tcccccgtat ttataaatat tttcgcaaaa gcgaaagttt ttttcaaccc
     4501 gtttatagaa attttttcaa taattaaaac gaaagcgggc aaaaaacatt ctgttttttg
     4561 cccgctttcg ttaaatcttt tctatatcaa atctaatgca gaaaatcttt ttcttcttga
     4621 tgtgaatttt aataacattg ctctcattgt ttttcctctt tctttatata taaagtatat
     4681 acagttgcgg aaattgcctt tttgtaaacg aatattacaa aataatttca ttgttttttg
     4741 tccttgaatg tgtagaatta atttaaggtg ggagttatgt taatacaagg tccaatttct
     4801 agtgaaaaaa cacaattgct gacacaaaag tatgccgagc ttttaaactc gggagtggac
     4861 agcactaaaa ttcttgtttt gctgcaaaat tcgaacaaaa aagaaaaatt tattaaagaa
     4921 actcttgaaa atttaaaaat agaaactgtt gaaaagcttc agatttattc gttttacgga
     4981 cttgtttaca acacaatcac cgataactgg gcatttttgg aaaattcaat taaagactca
     5041 aacacaaaaa ttcttccaaa tcttgtaggg ctggaagttt ctcaaagcct cctcaaagat
     5101 ataatcaaag acgttaaatt tgaaggatat aactccaaaa aatcactcct gcaccagctt
     5161 tttcgcagat attcattaat tgtccaaaat aacttaaccg atgttgatgt aaaaaaacgc
     5221 tccgaagaaa ttttgaaaga aagttttgcg cctgacgcta aagcggcact tgacgaattc
     5281 aaaaaaagaa cccttgaatt gagaagtttt gactacttaa gacaaagtct catttttaac
     5341 cacttgtata aaaatacagg ctgtttttcc aaaatcgaat atttgctggt tgatgacggc
     5401 gatgaaataa ccccgatttg tcttgatttt ataaagtttt tacagccaaa attaaaagat
     5461 attttcatag cttacgaccc tttgggttca agtcgttggg ggtatttaag tgcggataaa
     5521 agtgcggttt tcggctttga agaaatgtca aatgaagcac cgttggtttt gcctgcgaaa
     5581 actgccctta aagaagatgc acagactatt tttaataacg caacgggaca aaaaaaccaa
     5641 aagttaataa atttttccct gttttcaacc tcaaaacgtg ctcaaatgct tgatgaaggg
     5701 attaaaaaag taaaaagtct tatcaacaat tcaataaaac cttgtgaaat tgcaattatt
     5761 actccgataa tcgacgatat gctcaaattt tcgctaagag aaaatttacc caaaagtcag
     5821 gttaatcctg ttttcttgtc aggaagcgaa aaaattgtcc aaaatcccat tgttgcttat
     5881 tgtctttcca ttttaaaact ctgtgacgaa acgctcaaac aagagctttg tgagtttgaa
     5941 ataagggcaa ttctgtcgga tttcctgaaa attcctataa aatactgcag aaaaatcctt
     6001 gatgaatttg aaaaaacaaa ggaatttata ccttttactt ttaaaaatga agcgttcaca
     6061 aaaaattatg cagctttttt aaatttgtta gaaaacctca aaaaaacaaa caagctttca
     6121 gagcaaattt atgggatttc agagttaatc gacttcaaat tttacgataa ggatgaaata
     6181 aataaattta actttttcat aaaacaaatt gaagattttg aaagtgtttt taccgacaaa
     6241 aaaatctcaa acagtcagat aataaaccag attgaaaatt ctattatctc tgaaaatcct
     6301 tattctacgc ttgaaataga agaaaatgac cttgtaatcg caacgcctca aaaaattatc
     6361 gacaatcaaa ttcagacaaa atatcaaatt tggcttgatg tttcaagcga tgaatggata
     6421 aaaagcgaca caggcccgct ttataatgcg tgggtttttc aggcaggctg ggataaggcc
     6481 gaatttacgc ttcaagacaa cattgaactg agcaggcaaa aaaccgcaag aattttaaga
     6541 aaacttaccc tatgcgcagg tgaaaaagtt tacacctatg caagcctttt tgattctatg
     6601 ggtgtcgaaa atttcggcgg cattgaagga ggggtaaata taggaggggt aaatgagggg
     6661 gtaaatgcag ggggggtaaa tgagggggta gcagaagcaa aagttaaaaa aataattccc
     6721 cgcgacgacc aaaaggcggt tttggattac aaatcaggct caatggcaat ttctgcagtg
     6781 cctggggcgg gcaaaaccac cattttgcta tcgttaatcc tgaagcttct tgaagaaggt
     6841 acaaaccctg aaaacatttt tgtactgact tatatggaat cagccgcaag aaatttcagg
     6901 gaaaggattc agcttgcaaa cccttacagc acacagcttc ccaatataag cacaattcac
     6961 ggacttgcgc ttaaaatatt aaaagaaaat tcaaactttg agaaattggg cttaaatccc
     7021 gattttgaaa tctgcgacga cacccagcgg tcaaggattt tgagagaatt atctgcaaaa
     7081 cttaaactca aaaaaacaga atcagaagat tttgacagag ccgtttctgt tttgaaaata
     7141 agcaaaggaa caaacaaaat tgaaaataaa aaagcggaat taagtaaatt cctcgaattt
     7201 tttaacgaat acgatcaaat tttaaaagaa aacaacttaa tcgactatga cgatatgctt
     7261 attttgtcgg tgaaactttt gaaagaaaat cccgatgttt tgaaatatta tcaggacatt
     7321 tgcctttatg tgattgaaga tgaggcgcag gattcctcgc aaattcaaca ggaattaata
     7381 tctcttttaa gttcaaaaca taaaaactta atccgatgcg gcgatgtaaa tcaggcaatc
     7441 acaacgactt tttcaaacgc agatgtagaa ggtttcagaa gattcattaa tcaagcgcag
     7501 acaaaagtca gcatgaaccg ttcgcaacga tgcacgaaag aggtttggga acttgccaac
     7561 aatctgctca aaacatcaca aaaaaatcca aaaacccaaa acgctttcta tgaaattttt
     7621 atgcgcccgg tcgaaggaaa aaatccgcag gaaagaaatt cgattgtgcc catagttttt
     7681 gacaacgaat ttacagaaaa aaactttatc ctcaaacaga ttaaaactct tatttcaaaa
     7741 aatcccgaca aaactttggg aattcttttg agaaacaatt atcaagtcac aaattggaca
     7801 aaatttataa acaactcggg gctcaaaacc attacaagaa gcgaatgtct ggaacaaaaa
     7861 ggaattttca ggacaatttt tgcggtgctt aaaattattc aaaatccttt tgacaacgaa
     7921 aatattgccc aaaattacga tattcttgca gaactggggc actacacttc aggaatgttt
     7981 gcccagataa gaaatttccc ttcagttttt gtccagacaa gcaccgatga tattaaaaac
     8041 ctcgaccttg tcgattttta ctgggatgtg gattactggc tgaatttttc ttcaatgccg
     8101 attgaagaac ttgccattaa aatcgggctt tattactacc ccgacagcca aatcgaaaaa
     8161 tcaaacgtgt atcttattgc cacattaatt aagcgtttga gcgttcagta caaaaagttt
     8221 tccgctattt tagacaggct ctcagaactt gccaaaaagc ctaacctgag cggttttaag
     8281 ttcttttccg aagaagacga agacgacaaa aaattccttg aaggcaaagt ccagattatg
     8341 acgatgcaca aatctaaagg ggatgagttt gattttgtat ttttgcccga attcagcgag
     8401 gacagccttc cgattgaatt tgagaatatg tcgctgaaaa aaaattcaag atttatggaa
     8461 gaagtaagag aactgaatcc tgattacagg aaaaaaaccg atgacgattt aaaagaattt
     8521 ttgcttgcag aaaatttgag attgctttat gttgcaatta caagggcaaa aagaaagctg
     8581 ttttttacgg taagcaaaat cacaaaacct tacggaaaag aaaaagaaca aaaaccttct
     8641 gcaatttttg aaaagctttt aaaaccaaga aattcggagg tgacagatga ataatttttc
     8701 acccaatatg ttaaaaaact ttgaccagtg tcagcaaaaa ttctgcttca agtacgtaga
     8761 aaaaatctct atgccgcaaa aaccagagat ttttgaaaaa ggtaaaaaaa tccacgctct
     8821 tgcaaattat ttcttgaata aaaacgatat ttcaaaaatg gaacaaacgc ttacacaagc
     8881 agaaaaaacc gattgggagg ctttgaaaag taataaatat atgagctttg aacctgttgt
     8941 gacagaacat aatctttcgt gcaaaatcgg agatttttgg gtgggcgggc gtttggatgc
     9001 tattgtaaaa caaggcaatg attatttcat tttggattac aaaaccggaa acatacctca
     9061 aaatccgcaa aatgattttc agacaatggt gtatctgctt gccgcggaca aaatttttaa
     9121 acagaaaaat attcctgtca attcgattac ttttgtttat atagggctta aacagggtga
     9181 agaaaaatca attttattta acgaagattt aaaaaaaact tacgaagaaa atttaaaaga
     9241 aatttgcaaa aaaattgatt ttacatgcga tgcaaaagtt ttttcaaaaa cgcttacagc
     9301 ctgcaaatac tgtgaataca acaaaatttg ttataaagat taacaactac ctctcacccg
     9361 tcgttgcgag ggggaacaat atatttaccc ccgaagcaat ctaaaaagcg ttaaatttaa
     9421 gttattgtga cttctttctt ttttatatcg acgaaaaaag aaagaagtca ccaagaaaga
     9481 aaaatcactc ttttggctgc gccgcctgct cgagcgagct ctcgctaaat aacgggttgc
     9541 ttaatgaaaa attcattaag caactaaagg gtttcagagt gaaaccctct acggactctt
     9601 ttccgaccat atcggagtga gcaatttaca cttaacgaaa caacggcaga gcgagtgtgt
     9661 ttgagtgccg aaggcacgag ttcgcgagct cgggttgagt ttagtgtaaa ttagcgagcg
     9721 cagatccggt cgggagtttc tttgcctact ttcttgtcga cacaagaaag taggttcata
     9781 aaaaataatt acaaaaatta ttagtttgtg agatcctgaa tcaagttcag gatgacgtta
     9841 ttatttgatt attatatcac atttaaatat cgttaataaa aatccccctt tgaggagctt
     9901 ttatgacaga gtaggcgaaa tgcaatgccc gaaaatttca ctgtttgagc gtagcgagtt
     9961 tgaaattttc aatggaatga gccgtactct gggaaaaagc gacgagcagg gggtggcttt
    10021 tggcgggacc ttttgccacc aaaaggtccc aaaagaaaat attcaagaaa ttataacttt
    10081 ttagattgct tcggtggaca ataactataa aagccgataa cccacgtctc gcaatgacgt
    10141 aaaaaggata attatttatc cttatttaat gtaaattttg taaataattt tacaaaaaag
    10201 gcaattttgt agttaaaaac aactttatat atatgtaggt agtgtatatt tataggttgg
    10261 aaaatgacaa acgcagttgc atcagcaata ttttgtgcaa gaaacgtcga taaaaccaaa
    10321 catggtgaag tcggccgaag tgctgttgcc gtagggcaag caaaaaaggt catcgattca
    10381 attatacaac tggacaaaaa gcttggcaag ggtcccgcaa aagctgttga aaatattctt
    10441 aacttttcaa ataaaagcaa agctttacaa gcaataggaa aaatcacaaa atttgcaagc
    10501 gataacatta accctcttat ttgcgtttct tccggtatta aagttttaac ttcggatgat
    10561 aaaaaaacaa ctctaataaa tgaaacaggt gccctttcag gaatgtttat atcagaaaaa
    10621 gttgccaaaa agttgctgga taatatggtt gaaagcaaaa cagtaaaaga actttgcgaa
    10681 aaaggaataa aatctgccga tgcaaaaggc tataggacac ttagcaaatt gattaaatca
    10741 tctccgacaa tattaaaagg tattttgttt gtatttgcat caatcggcgg atacagcata
    10801 ggctcaaaaa ttgcaaagaa aatttcttca aaagaaaaaa aacctgcaaa aaacgaaaat
    10861 atttttgcaa aagcccccct cgcagaacaa aaagtgcaaa acaaagctcc tgaaaaaatt
    10921 gaaaaaacag tccaaaaaga agaagttgaa cccaaaaaag tagatgttgc ggcatagtca
    10981 aggcagagca aaaagccctg ttcacttgac atatagtttt ttcgttaata taatattctt
    11041 aatcacttgc cggtgtagct caacggtaga gcaacggttt tgtaaaccgt aggttggagg
    11101 ttcgattcct ctcaccggct tactttttct aaagaattga aaaagtaaac cttgaatttt
    11161 gaactccatt cactgttcac cattcactaa tatgcccttg tggctcaggg gtagagcact
    11221 cccttggtaa gggagaggcc acgagttcaa atctcgtcaa gggcaaattg ataattaaat
    11281 aatgttccaa gttcaaagcg aagagtgttc aacaatgtac cctcgctaaa gtaaggctcc
    11341 ttgtcagttg taaacaaaac gacactcggg gctacgggaa acaaagtttc ccttcgaatt
    11401 tgggtaggtg cccgagtggc taaagggagc agactgtaaa tcagtgttgg agaggctaaa
    11461 agcctctttt ttagtgggtt ttgaacaaaa catactacct tttgaacagg gctgaaagta
    11521 ttgattttac gttgaacaaa atacacaact ccacaaatat tacacaaaaa attttgaagc
    11581 tgattttata cgtattttaa aaatgcgcag attaagatac tgcgagtact gaaattagaa
    11641 catttttaaa gttcaactta ttttcatgcc tgaattgtga aaattttctg ctataataaa
    11701 acggtaagga gattaagaat tgactttaga cttgtccaaa aatgaaagtg ttattttaga
    11761 atataaagaa gaaattaata ctaattttta taaatcatta tctgcttttg cgaatacaaa
    11821 aggcggaata attcttttag gagttaccga caaaaaagaa cctaaaggta tagatttacc
    11881 aaaaggaaga caagaaacaa ttattaatca aatagtagat tctcttggca ttcaacctga
    11941 aattaatata ttaacaattg aaggtaaaaa tattttatct gtcttagttg aaaaatcttc
    12001 taatccgatt tcttataaag gtaagtatta caagcgtgtt gggaatacta caagagagat
    12061 gcaaagagaa gaattaaaga aaatgttctt aaaagatgtt tcttgggata ctttgacagg
    12121 caactactct attgatgaaa ttgatacgga aatagtcaga tactttatta aacttgcaaa
    12181 cagtaagaaa agattgccta tagtcgggga agatgaaagt atacagtcaa ttcttgaacg
    12241 tttagattta attattgacg gtaaattaac caatgcctca atattgttat tcggcaaaaa
    12301 tccacaaaaa tactttacaa gtgcaacttt aagagtcggt gcatttaaag ataatactgg
    12361 agttacattc attggagaca gacagataaa gggaaaccta tttaagcagg ctatagacgg
    12421 agaagaagcc ttaaaatttt ataataatgt taaatacgtt atttcaggga agctacaaag
    12481 ggatgaatat tgggattatc ctttagatgc agtcagggaa gcgttattga acgctttaat
    12541 acatagagat tattttgata gtggtacaga aatacaagca aaaattttta aggattctct
    12601 ttggatatat aacagtggag gattaacagg cggtctaact ttagagcaaa tcaaaggtca
    12661 acacccatca cgttcaagaa acaagctaat aatgaacatc ttttatttgt cgggcatggt
    12721 cgaaagattt ggctctggta tcagtagaat aattgagaag tgtaaatctc aattattacc
    12781 tgaacctgaa tttaaagaag agtctggtgg cttctcagtt tactttagaa aagaattttc
    12841 aggcttgaat gatagacaga aaaaagctat agagtttatc agagaaaaag gtgctataaa
    12901 taacagcttg taccaagaat taacacaagc cacaagagaa acaagtaaaa gagacttatt
    12961 taaaatggta gaagtgggga ttattaaaaa agaaggtgaa ggtaagctta caaagtatat
    13021 cttaccttaa tttatataaa cggatataaa gtgcataaac atacataagc agacataaac
    13081 gtatataatg acccataaag acccataaat gattatttgt gtatatgtgt atatacttat
    13141 atacacaggg ctagcatgct agattgatag aatgctaata tcagacatga cccataagct
    13201 ataaactatt tatcttattt taactttgtc tttataggct acacgcagca tcttttacac
    13261 aaaatttcac accatttaca catagaaatt tggtaataaa tttcaaagat aggtagagaa
    13321 aagaagttat attataccca taatttaaaa actattaccc tattttttgt agctttttta
//
LOCUS       DAOQAU010000045        13151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5215_length_13151_cov_6.734575, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000045 DAOQAU010000000
VERSION     DAOQAU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_13151_cov_6.734575"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /gene="serA"
                     /locus_tag="PKI94_06800"
     CDS             <1..1454
                     /gene="serA"
                     /locus_tag="PKI94_06800"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNW26479.1"
                     /translation="MVRSETKVTSKVIEAGKNLKIIGRAGVGVDNIDVEAATQKGVIV
                     VNSPDGNTNAAAEHTIALMLSMARNIPKASQSTKMGLWERSKFTGVEVFGKTLGIIGL
                     GKIGSHVAHVATALGMKVLVCDPYTSKETVEAIGAQYVSKLDDFWAKCDFITIHAPKT
                     KETTHLINKDTISKMKKGVRIINCARGGIVDESALKEAMESGQVSAAAIDVYEDEPNI
                     KNSPLINIEGNIVLTPHLGASTAEAQLNVAIDVANQIKEVLTGGSASSAVNIPSLKPD
                     KLEPVKDYMQIAQNIGEFAMQIVSGNLKSIEITATGNLTSLDISPLEVAIIKGIFSSF
                     VENVNFVNAPVLAKQKGISVTTSKTDKTCNYIGLLSVKLITDIQEATVSGALVAENMQ
                     RIVKINQYTTSIEPEKHLILVPHENKPSMIAQVATVIGGHGININHMHVVQKTGENSI
                     MIINTDKAVDSDVLDKISKIKGVCSSKYIKLST"
     assembly_gap    1567..1666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1667..1938)
                     /locus_tag="PKI94_06805"
     CDS             complement(<1667..1938)
                     /locus_tag="PKI94_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNW26480.1"
                     /translation="MNKEELVQEIAKKAKVTQKDAGEVLGALVDTIQKTVAKGKKVTL
                     VGFGTWEPRKRAARNGRNPQTGKEIKIAAKTVPAFSAGKKFKQIVNG"
     gene            2084..3283
                     /locus_tag="PKI94_06810"
     CDS             2084..3283
                     /locus_tag="PKI94_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26481.1"
                     /translation="MIFRDEFNKIARLFKPKDKLLNKIYFSISSEGYGHSSRAIAIST
                     KLDKKNYLIGTYGYALERLKEHGIPCQKISQELEFVGQDGAFDVAKTIVKNQGWVVKF
                     NKLVDEEVDIIRNSGASCVVADGRMVPVMAAEKLKLPCITITNQSAFYPFFQQDNWLV
                     KLLGVSFDSWMRMYLSSTEEILIPDFPPPYTVCLPNLSHNRKIMKRTRFVGPLVNFDI
                     NSVPLIQRNTDKPYVVVSLGGHAYRRPLFDCILAVAERMPEVDFEIFTFFEAEKIPSN
                     VSIKGMIGDISPYLKTADLVIAQAGHSTAMEILSLGKPSVIVPDLNQIEQENNARRMA
                     ELQVSEVVTHKNLTVQKLYDCTQKVMKDDKYKKHAQFFATMSLELDAKSKVADILSEY
                     AMRLRRY"
     gene            3284..4630
                     /locus_tag="PKI94_06815"
     CDS             3284..4630
                     /locus_tag="PKI94_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW26482.1"
                     /translation="MRERIVLFAIIFGLGVFLYVFQNYFNTLWGLVFLCCAMLVYAFY
                     MHLATLHKRRQLRKNPQVINENFKPFVTIMIPSHNEEGVISKTVENVLAITYEKFEVI
                     VIDDRSDDNTASVIKDLEKKYDKVTAMVRPKDAYPGKSAVLNEAMALAKGEAILVFDA
                     DARVQPDFLSRLVPHLESEDVGAVQARKVISNKDYNFLTRCQNNEYTLDTHFQVGRDA
                     VKGAVELRGNGELIKRAALEDIGGWNNYTITDDLDMSTQLHIKGWDVRFCSDTVVYEE
                     GVVYLIPLFRQRRRWLEGSIRRYLEHFGDVLNSKEMSLRASLDMTAYISEFLMPIWLL
                     MEIFFQVGRVATGVKPINYAFSSLTMGLAIAVCFVLAIRYSLRRYDNVPRSKALKQAI
                     ETALYMLVIWFPVVMWICFKILFCKKDMNWGKTTHGLVLEEEQHLKAQELVCQVNK"
     gene            4737..5270
                     /locus_tag="PKI94_06820"
     CDS             4737..5270
                     /locus_tag="PKI94_06820"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNW26483.1"
                     /translation="MTQQVIEQVKDTIRSIKDFPKPGIMFRDITTALKDKQTLRLMVD
                     FLCEQFKDEKIDYVVGMESRGFIFGMPVAYKLNAGFIPVRKPNKLPAKTIKETYELEY
                     GTDALEIHEDALKKGDRVLILDDLLATGGTAAAACNLVTRTGAEIVGCGFVIELKALN
                     GRAKLPKDVRIVSMVKY"
     gene            complement(5277..6740)
                     /gene="gatA"
                     /locus_tag="PKI94_06825"
     CDS             complement(5277..6740)
                     /gene="gatA"
                     /locus_tag="PKI94_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNW26484.1"
                     /translation="MKELLKKSAAQQHKMIKNKEISAVELIQATFERIDEVEDKIGSF
                     NCLTKEVAFETAKKVDAKVANGEELPLLAGVPLALKDNMNLIGYPTTASSKILENFIS
                     PYNATITEKLLNNLVPIVGKANLDEFAMGSSTENSAFKKTYNPWDLKKVPGGSSGGSA
                     ASVAACEAALSLGSDTGGSIRLPGSFCGIIGMKPTYGRVSRYGLIAFASSLDQIGPFA
                     RTIEDAAMLLEVIAGLDPKDSTSLDVPVEPYSKMLDTDIKGMKVGVIKELLAEGVTDD
                     VKKAIENAIDFYKSAGCEIKEVSLPYLKYSIGIYYILATAEASSNLARFDGVKYGYRV
                     KDAKNLMEMYTKTRAEGFGPEVKRRIMLGTYALSSGYYDAYYRKAQQLRALVKQDFDR
                     VFKEVDILVSPTCPNTAFEMGARADDPLAMYLTDIATISANLAGIPGLSVPAGFDSSD
                     MPIGLQILAPVLQEVKLFNAAYKFEQAHDFYTKIAQV"
     gene            complement(6798..7484)
                     /locus_tag="PKI94_06830"
     CDS             complement(6798..7484)
                     /locus_tag="PKI94_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26485.1"
                     /translation="MDIEKASGNNFFTAAPLDNAQGNFFAAFGNVQNSIGQKKQSPHR
                     LNDYDSNILENTAYQKISDDMLKIEHRIGILEDTLTKITAEIEALKSFGDSIQINSLI
                     DKKQLIEEEIEELNRRYSELGLSAKISGQIASVIRLSSKNKAGIFSKIKNFVSKNILA
                     RFFKKINYRENIKDALVKLSSINSNVDDLITMHTPYGETFSRYEKLTAYINKANSIHA
                     QISKNLKEIK"
     gene            complement(7572..8771)
                     /gene="nifS"
                     /locus_tag="PKI94_06835"
     CDS             complement(7572..8771)
                     /gene="nifS"
                     /locus_tag="PKI94_06835"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNW26486.1"
                     /translation="MTRKMVYLDNNATTKVDEKVLEAMLPYLKQNYANPSSIYSFGGI
                     SAQAIRKARVQVRDFVGAKSEKEIIFTSCGSESANTAIKGVLDVNKTKKHIITTKVEH
                     PCVLSLYKTLEKQGYKVDYIGVNANGELDLDELENAVNADTALVSCMWANNETGIIFP
                     IKKISHIIKSKNKETKLFVDGVQAAGKIPMNVEDAGIDLLAVSGHKFHAPKGVGALYV
                     KSDTLISPLIIGGHQERGKRAGTENVPYIVAIGEAAQLAQDALDFEATTVKHLRDKLE
                     NSLIKNVFNARLNTSLNNRVPNTTNIGFEYVEGELILLHLSDMGICASSGSACTSGSL
                     EPSHVLKAMGIPFTALHGSVRFSLSRFTTEKEIDYVISVVPRVIDKLIKISPFQEELA
                     TLRKHKS"
     gene            complement(8789..9619)
                     /gene="nifU"
                     /locus_tag="PKI94_06840"
     CDS             complement(8789..9619)
                     /gene="nifU"
                     /locus_tag="PKI94_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNW26487.1"
                     /translation="MWDYTEEVMDHYRNPRNVGKIDDADATGEAGSLACGDALKIYLK
                     IDKNNIVTDAKFQTFGCGSAVASSSVLTEMIIGKSIDEVKKITNKDIADKLGGLPPEK
                     MHCSVMGHEALEDALKKYLGEETSEAKKDKLICKCFSVTENQILNAIKENNLKTVEDV
                     TNYTKAGGACGHCKGDIQAILNKFYNKKTKTEEVPKLTAAQKILKINTIIENRISPEL
                     RKDGGDIELVDIDGNKVYVKLRGACSGCKNSTLTLKSFVEMTLRELLKEEIEVIEVQN
                     "
     gene            9814..10164
                     /locus_tag="PKI94_06845"
     CDS             9814..10164
                     /locus_tag="PKI94_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26488.1"
                     /translation="MNIMPVIPSIKTSTSYRPKFGNNSEEVAVRHENPCKNFWEGKDC
                     YDNLIERKQEIIIELRNNLEELKRLSNISPCFHTDDCDCVSVMSSIQEYKENINALNA
                     ELKDINKQLKTFKN"
     gene            complement(10144..11055)
                     /locus_tag="PKI94_06850"
     CDS             complement(10144..11055)
                     /locus_tag="PKI94_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNW26489.1"
                     /translation="MNLIKPEKLKKGDTIGFIALSGTVEKEENIYRAKEYFEKKGYKV
                     VFSKNIFDKNRYLAGKDEDKIKELHRFFKDKNINAILCARGGYGTIRLLDKIDFELIK
                     NNPKIFAGYSDTSALQAMILKKTGLITFYSPMAQSDFGVEEVSKVTEKSFFETLQNVG
                     ELMLLPERKEAKVYFEGEAKGVLFGGNLATLVSLCGLDFIPSEKFILFIEDLNEPVYK
                     IDKMMTQLLNIEKFKKNISGIILGDFLKNGNKKYFDELFFEIGEKIKKPILSGFKITH
                     AHEKITLPYGAQGYLSTKSKKLIFKSF"
     gene            11266..13113
                     /gene="dnaX"
                     /locus_tag="PKI94_06855"
     CDS             11266..13113
                     /gene="dnaX"
                     /locus_tag="PKI94_06855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNW26490.1"
                     /translation="MSEKYVPLYRKYRPQTLEEIVGQEHIKKSLANAITLNKISHAYL
                     FTGPRGTGKTSTARILAKSLNCIQGQSVTPCGLCPSCIEITNSIPVDVIEIDAASNRK
                     VEDARNILEKIQYVPVNGRYKIYIVDEVHMLTKEAFNTLLKTLEEPPENVIFILATTE
                     PHKVLETITSRCQRFDFRRITTDDIIAYLKEIAQKEKINIDNDALFTIAKNSAGGMRD
                     SLALLDQVSVLGDSQKINAEDVNNLLGRLSFDLLHKLSNNIINSETQEAIECLERIYN
                     SGNEPVQIMLNLLGYFKNLLIVKNCTDKTLLIDLTQLNDSQIKILQEQMQKAEIHQIV
                     FLIERISYYIKELKTTNAQHLWLEVAMIDLANMAQNSSLIALQERLSRLETSGVSANP
                     QQYTTQPAPIPTEPIKSVTENAKQTESVKKDEKISVEKVIQKIEEKLEIKDFSPMPKT
                     EPKPQPKQQNKDNFMESWNQFLLLLKQKSPPTYAMVSQHSIPVEFSPQKVTIACRNSL
                     LLNSDEKKKSIKNVAEEFFSSQDISVVIRLQSDNDKIIEPTTAPVEKKKLNETAQTQS
                     ENLPEEEIQEENLEEIVSTENLPEKTTQKVMMSDQVNMVMGLFDGKYID"
BASE COUNT         4174 a         2365 c         2351 g         4161 t
ORIGIN      
        1 ttatggtcag aagcgaaacc aaagttactt caaaagtcat tgaagcgggc aaaaacctga
       61 aaatcatagg cagggcaggt gtcggcgtgg ataacattga tgtcgaagcc gccactcaaa
      121 aaggtgttat agtggtaaac tcccccgacg gcaacacaaa cgccgctgca gagcacacaa
      181 tcgccctgat gctttcaatg gcaagaaaca tacccaaggc ttctcaatcc accaaaatgg
      241 ggctgtggga gcgttcaaaa ttcacaggag tcgaagtctt cggaaaaacg ctcggcataa
      301 tcgggctcgg aaaaataggc tctcatgttg cgcatgtagc gacggcttta gggatgaagg
      361 ttttagtctg tgacccttat acttcaaaag aaacggtgga ggctatcggg gctcaatatg
      421 tttcaaaatt ggatgatttt tgggctaagt gtgattttat aacaattcat gcgccaaaaa
      481 caaaagaaac gacgcacctt atcaataaag acacaatttc taaaatgaaa aaaggagttc
      541 ggattataaa ttgtgcaaga ggtggaatag ttgatgaaag cgcacttaaa gaagctatgg
      601 aatcaggtca ggtaagtgct gcagcgattg acgtttatga agatgagcct aatatcaaaa
      661 attccccgct tataaatatt gaaggaaata tagttttaac tccgcatttg ggtgcaagta
      721 cagcagaagc ccagcttaat gttgcaattg atgttgcaaa tcagataaaa gaagtcttga
      781 cgggaggctc tgcctcaagt gcggttaata ttccttcttt gaagccggac aaacttgagc
      841 ctgtaaaaga ttatatgcaa atagcacaaa atatcggcga atttgcaatg cagatagtca
      901 gcggaaatct aaaatcaata gaaataactg caacgggaaa tcttacttca cttgacattt
      961 ctccgcttga agttgcaatt ataaaaggaa ttttttcttc ttttgttgaa aatgtcaatt
     1021 ttgttaatgc tcctgtttta gctaagcaaa aaggaatttc tgtcacaacg tcaaaaaccg
     1081 acaaaacctg caattacata ggacttttaa gtgtaaaatt gattacagat attcaagaag
     1141 caacggtttc aggagctttg gttgcagaaa acatgcaaag aatagtaaaa attaaccaat
     1201 acacaacaag tattgaacct gaaaaacatc tgattttagt gccgcatgaa aataaacctt
     1261 caatgattgc acaagttgca actgtaatag gcgggcatgg tattaacata aatcatatgc
     1321 atgtcgttca aaaaaccggc gaaaattcga ttatgattat aaatacagat aaagccgttg
     1381 atagtgatgt tttggataaa atcagcaaaa ttaaaggcgt ttgctcctca aaatatatta
     1441 aattaagtac gtaaagattg cttcggtgga tgctttaagg attatctgct aattcttgtc
     1501 tcgcaatgac gtaaaagaaa ggtgtgtaat taattacaca ccttttaaaa aattaacaac
     1561 cgttaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgt taacgatttg
     1681 tttgaatttt ttaccagcag agaatgcagg tacagtttta gctgcaattt taatttcttt
     1741 accggtttgt gggtttcttc cgtttctagc agctcttttg cgtggttccc aagttccgaa
     1801 tcctaccaaa gtaacttttt tgcctttagc aacagttttt tgaatagtat ctaccaatgc
     1861 tccgagaact tctcctgcat ctttttgagt aactttagct tttttagcta tctcttgtac
     1921 taattcttct ttgttcataa taataaaccc ctttcttaat gtgtacatgg actattatat
     1981 tgaaactaaa ataaaacgca agtattttta cgaaaattaa tataattttt cccaaaaaaa
     2041 catatatttg gactataata ttgttggaag aggtaagtta aatatgattt tcagagatga
     2101 atttaacaaa atagcaagac ttttcaagcc aaaagacaaa ctcttgaata aaatttattt
     2161 ttccataagc tcggaaggat acggccattc ttcaagagcc atagctattt caaccaaatt
     2221 agacaaaaaa aattatctta taggcactta cggctatgct ttggaaagac ttaaagagca
     2281 tggaattcca tgccaaaaga tttctcagga attggaattt gtaggacaag acggtgcttt
     2341 tgatgtcgca aaaacgatag ttaaaaatca aggctgggtg gtaaaattca ataaacttgt
     2401 cgatgaagaa gtcgatataa taagaaattc aggggcgtca tgcgttgttg cggacggccg
     2461 catggttcct gtcatggcgg ctgaaaagct taaactgcct tgtattacaa ttacaaacca
     2521 gagtgctttt tatccgtttt ttcagcaaga taactggctg gtcaagcttt taggtgtttc
     2581 ttttgattcc tggatgcgca tgtatttaag ttcaaccgaa gaaattctta ttcctgattt
     2641 tccgccacct tatacggttt gtttacccaa tttaagccac aacagaaaaa tcatgaaaag
     2701 aacccgcttt gtaggacctt tggttaattt tgatattaac agcgttcctt taatacaaag
     2761 aaacactgac aaaccttatg ttgttgtatc cttgggcggg catgcctata gaagacctct
     2821 ttttgattgt attttggcgg tcgccgaaag aatgcccgag gttgattttg aaattttcac
     2881 tttctttgaa gcggaaaaaa taccttcaaa cgtaagcatt aaaggcatga ttggagatat
     2941 ttcgccttat ttaaaaaccg cagaccttgt cattgcccaa gcaggacatt ctacggctat
     3001 ggaaatttta tctttgggta aaccttctgt tattgttccg gacttaaatc aaatcgaaca
     3061 agaaaataat gctcgaagaa tggcagaatt acaagtctca gaggttgtta cgcacaaaaa
     3121 tttaactgtt caaaaacttt atgattgtac acaaaaagta atgaaagatg acaaatataa
     3181 gaaacatgcc caattttttg ctacaatgtc attggagctt gatgcgaaaa gcaaggttgc
     3241 cgatatttta agtgagtatg caatgaggtt acgcaggtat taaatgagag aaagaattgt
     3301 tttatttgca ataatttttg gacttggagt gtttttatat gtttttcaaa attatttcaa
     3361 caccctatgg gggcttgtat ttttatgctg tgcgatgctc gtatatgctt tttacatgca
     3421 ccttgcaact cttcacaaaa gacgccaatt aagaaaaaac ccgcaagtaa taaacgaaaa
     3481 ttttaaacct tttgtcacga tcatgattcc ttcacacaat gaagagggag ttatttcaaa
     3541 gacagttgaa aatgttttag ccataactta cgaaaaattt gaagtcatag taattgatga
     3601 cagaagtgat gacaataccg catctgttat aaaagactta gaaaaaaaat acgataaagt
     3661 aacagcaatg gttcgcccaa aagacgctta tcctggtaaa tctgcagtcc taaatgaagc
     3721 tatggcactt gcaaaaggtg aggcgatact tgtgtttgat gcagatgcaa gagttcagcc
     3781 tgattttctt tcaagactcg tgcctcatct tgaaagtgaa gatgtcggag ctgttcaggc
     3841 aagaaaggtc atcagcaata aagattataa ttttttaacc cgctgccaaa ataacgaata
     3901 cactttggat acacactttc aagtcggtag agatgcggtt aaaggggcag tagagcttcg
     3961 cgggaacgga gaattgataa aaagggcagc actggaagac atcggcggct ggaataatta
     4021 tacaattacc gatgatttgg acatgtcaac tcaacttcat ataaaaggct gggacgtaag
     4081 attttgttcc gacacggttg tctatgaaga aggagtagtt tatcttatac cgcttttcag
     4141 gcagcgcaga aggtggcttg aaggcagtat cagaagatat ttagaacact ttggtgatgt
     4201 tctgaattca aaagaaatgt ctttaagagc aagtcttgat atgacagctt atatttccga
     4261 attcttaatg ccgatttggc tcttaatgga aatatttttt caggtcggac gcgttgcaac
     4321 aggagttaag ccgataaatt atgcgttttc ttcattgaca atgggacttg caatagctgt
     4381 ttgctttgtt ttggcaataa ggtatagtct tagaagatat gataatgttc cgagatcaaa
     4441 agccctgaaa caagcgattg aaacagcctt atatatgttg gtaatatggt ttccggttgt
     4501 tatgtggata tgttttaaaa tactgttttg caaaaaagat atgaattggg gaaaaacaac
     4561 tcacgggctt gttttggaag aagaacagca cttaaaagca caggaattag tatgtcaagt
     4621 gaataagtga ataggtaaat aggtgaatag gttcaattat ttaaaaattt aaattatata
     4681 aaatgcactt attcacttat taacttattc acctatttac ttagaaaagg agaaaaatga
     4741 cacaacaagt aatcgaacaa gttaaagaca caatcagaag tataaaagat tttccaaaac
     4801 cagggattat gttcagagat ataacaaccg cgcttaaaga taaacaaaca cttcgattaa
     4861 tggtggattt tctgtgcgaa cagtttaaag acgagaaaat tgattacgtt gtcggaatgg
     4921 aatccagagg ctttattttt ggcatgcccg ttgcatacaa gcttaatgca ggttttattc
     4981 ccgtcagaaa accgaataaa cttcccgcaa aaacaataaa agaaacttat gagctggaat
     5041 atggcactga tgctctggaa atccacgaag atgcgcttaa aaaaggcgac agggtgctca
     5101 tattagatga tttacttgca accggaggaa ctgcagctgc agcgtgtaat cttgttacaa
     5161 gaaccggtgc tgagattgta ggatgcggct ttgttattga attaaaagca ctaaacggca
     5221 gagcaaaact tcctaaagat gtcagaattg tttctatggt gaagtattag aaaaatttaa
     5281 acctgcgcga tttttgtata aaaatcgtgt gcctgctcaa atttataggc cgcattaaac
     5341 aattttactt cttgaagtac aggtgctaaa atttgcaatc cgattggcat gtctgaagaa
     5401 tcaaagcctg caggaacact caaaccggga atccctgcaa ggtttgcgct tattgtcgca
     5461 atatcggtta aatacatagc aagcgggtca tctgctcttg cgcccatttc aaatgctgtg
     5521 ttaggacatg ttggagaaac taaaatatca acttctttaa acactctgtc aaaatcctgc
     5581 ttaaccaaag cccttaattg ttgcgctttt ctgtaataag catcgtaata gcccgaactt
     5641 aatgcataag ttcccaacat tatacggcgt ttaacttcgg ggccgaagcc ttctgctctt
     5701 gttttagtgt acatttccat aagattttta gcatctttga ctctgtaacc atatttaaca
     5761 ccgtcaaagc gtgccaaatt cgaactggct tctgctgttg caagaatgta ataaattcct
     5821 attgaatatt taagataagg cagtgaaacc tctttgattt cacagcctgc ggatttataa
     5881 aaatcaatgg cgttttcaat tgccttttta acatcatctg taacaccttc agctaaaagt
     5941 tcttttataa cgcccacttt cattccttta atgtcggtgt caagcatttt tgaatagggt
     6001 tcaaccggca cgtctaaaga tgttgaatct ttagggtcaa gacctgcgat gacttccaaa
     6061 agcattgcag catcttcgat tgttcttgca aaaggaccga tttggtccaa tgaagatgca
     6121 aaagctataa gcccgtatct ggaaactctt ccataagttg gtttcatccc gattattccg
     6181 caaaaacttc ccggcaatcg gatactgccg cctgtgtcag aacccaggga caatgctgct
     6241 tcacaagctg caacgcttgc agcagaacct cctgaagagc ccccgggaac tttttttaag
     6301 tcccaagggt tataagtttt tttgaaagca gagttttcgg tggagcttcc catcgcaaac
     6361 tcgtctaagt tggcttttcc cacgatagga acaagattgt tcaaaagttt ttctgttatt
     6421 gtggcgttat aaggagaaat aaaattttca agaattttgc ttgacgcggt ggtcgggtat
     6481 ccgattaagt tcatattgtc tttgagtgcg agcggaactc ctgcaagaag cggtaattct
     6541 tcgccatttg ccacttttgc atcaactttt tttgccgttt caaaggcgac ttcttttgtt
     6601 aaacaattaa acgagcctat tttatcttca acttcgtcaa ttctttcaaa agttgcctga
     6661 attaattcga cagcagaaat ttctttattt ttaatcatct tatgctgttg cgctgctgat
     6721 ttttttaaaa gctctttcat aatctctcct tagcctgtaa ctatgtcaat tatacttcaa
     6781 aaaaaagctt ttataaacta ttttatttct tttaaatttt tagatatttg tgcgtgaatt
     6841 gaatttgctt tattgatata agcagttagt ttttcgtatc ggctgaaggt ctcgccgtaa
     6901 ggagtgtgca ttgttattaa atcatccacg tttgaattaa tactggagag ttttaccaat
     6961 gcatctttta tattttcacg ataattgatt tttttaaaaa atcttgccaa tatatttttt
     7021 gatacgaaat tttttatctt tgaaaaaata cctgctttat tttttgacga caaccttata
     7081 acagatgcaa tttgtcctga aatttttgca cttaagccca attccgaata tcttctgttt
     7141 aattcttcga tttcttcttc aataagctgt tttttatcta taagcgaatt tatctgaatt
     7201 gaatctccga aagactttaa tgcttcaatt tcagctgtta tctttgtcaa agtgtcttca
     7261 agaattccta ttctatgctc aatttttaac atatcatcag aaattttttg ataggcggta
     7321 ttttccaaaa tatttgaatc ataatcgtta agtcgatgag gactttgttt tttttgtcca
     7381 atcgaatttt gaacattacc gaaagccgca aagaaatttc cctgcgcatt atcaagaggc
     7441 gcagcggtaa aaaaattatt tccgcttgct ttttcaatat ccatatactc aaattatatg
     7501 ggcattttat ttatatgata acctacactc ggagtatttt ggctgttcgt tccggcatga
     7561 cgccaatttg attaactttt atgctttctc aatgtggcca attcttcctg aaacggcgaa
     7621 attttaatca atttgtcaat gactctgggg acaacagaaa tcacatagtc tatttctttt
     7681 tcggttgtga aacggcttaa actgaaccgt acactcccat gaagtgctgt aaacggtatt
     7741 cccatagctt ttagtacatg gctgggctca agagaacctg atgtgcaggc acttcccgag
     7801 cttgcacaga ttcccatatc gcttaagtgc agtaaaataa gttcaccttc aacgtattca
     7861 aaacctatgt ttgtagtgtt cggtacacga ttgtttagag aagtgtttaa tcttgcgttg
     7921 aacacatttt taattaaaga attttcaagc ttatctctta aatgttttac agttgtggct
     7981 tcaaagtcaa gcgcgtcttg tgcaagttgg gcggcttcgc ctatggcgac aatataagga
     8041 acattttctg tgcctgccct tttgcccctt tcctgatgac cgccgattat aagcggtgaa
     8101 ataagagtgt ctgatttgac ataaagtgca ccgacacctt ttggcgcatg aaatttgtgt
     8161 cccgaaacag caagaagatc aattcctgca tcttcaacat tcataggaat tttacctgct
     8221 gcctgaaccc cgtcaacaaa aagttttgtt tctttgtttt ttgactttat aatgtgcgag
     8281 atttttttga taggaaaaat tattcctgtt tcattgtttg cccacatgca agaaaccaaa
     8341 gcagtatcag catttacagc attttctaat tcatccaaat ccaattcgcc gtttgcgtta
     8401 actcctatgt aatccacctt gtagccttgt ttttcaagtg ttttataaag gcttaaaacg
     8461 caaggatgct cgactttggt tgtaatgata tgtttttttg tcttatttac atccaaaacc
     8521 cctttgatag cagtatttgc gctttcagaa ccgcagcttg tgaaaattat ttctttttcg
     8581 ctttttgcac ccacgaaatc tctaacttgc acgcgtgctt ttctgattgc ctgagctgaa
     8641 attcccccaa aactatatat actcgaagga tttgcgtaat tttgttttaa ataagggagc
     8701 attgcttcca aaactttttc atcaaccttt gtagttgcat tattatccag atataccatt
     8761 tttcttgtca tttttaatcc ttttgacttt aattttgtac ttcaatgacc tcgatttctt
     8821 ctttaagaag ttctcttaag gtcatttcaa caaagctttt aagtgtaagt gttgaatttt
     8881 tgcaaccgga acaagcacct ctaagcttta catatacttt gtttccgtcg atatcaacaa
     8941 gctctatgtc gcccccgtct tttcttaatt ccggcgaaat acggttttct attattgtat
     9001 taattttaag aattttttga gctgcagtca gctttggaac ttcttctgtt tttgtttttt
     9061 tattgtaaaa cttattcaaa atagcctgaa tgtccccttt acagtgcccg caagccccgc
     9121 ctgcttttgt ataatttgtg acgtcttcta ctgtcttaag attgttttct ttaatcgcat
     9181 tcaaaatttg attttctgta acactgaagc atttgcaaat taatttgtct tttttagcct
     9241 cagaagtttc ttcgcccaga tattttttaa gagcatcttc caaagcttcg tgtcccataa
     9301 cagaacagtg cattttttca ggcggaagcc cacccaattt atctgcaatg tccttgttgg
     9361 ttattttttt aacttcatca atagatttac cgataatcat ttctgtcaaa acacttgagc
     9421 ttgcaacggc agaaccgcag ccgaaggttt gaaattttgc atcggttaca atattgtttt
     9481 tatctatttt taaatatatt ttaagcgcat ctccgcaggc aagagagcct gcttctcctg
     9541 tcgcatctgc atcatcaatt ttacccacat ttctggggtt tctgtaatga tccattactt
     9601 cttcggtata atcccacata tctttgtcct ttttaaatat ccgctttact cattttaccc
     9661 caaaaaactt ttatcctcac ctaaattaat tttaaattaa ttttttcaaa aaaatattaa
     9721 gttttgtata aattttgcct ataaactagc aaaaaaaatt attatgaggt ttatttaaat
     9781 tgaaaagctg aaagtgtttt aaaaggagaa attatgaaca ttatgcccgt tataccaagt
     9841 attaagacct caacaagtta tcgacccaag tttggaaata attctgaaga agttgcggta
     9901 agacacgaaa atccttgtaa aaatttctgg gaaggtaaag actgctatga taatttgatt
     9961 gaaagaaaac aagaaatcat aatagaatta agaaacaatc ttgaagagct taaaagactt
    10021 tcaaacatca gcccgtgttt tcacacagac gattgtgatt gtgtttctgt tatgagttca
    10081 attcaagaat acaaagaaaa tataaatgct cttaatgctg aattgaaaga tattaataaa
    10141 caattaaaaa cttttaaaaa ttaatttttt ggatttagtt gaaagataac cttgtgcacc
    10201 atatggcagg gttatttttt cgtgagcgtg ggttatttta aatcccgaca aaatcggctt
    10261 tttaattttt tccccgattt caaaaaacag ttcgtcaaaa tattttttat tcccgttttt
    10321 aagaaaatca cccaaaatta ttcctgaaat attttttttg aacttttcaa tattcaaaag
    10381 ctgggtcatc attttatcta ttttatagac aggctcgttt aaatcttcaa tgaataaaat
    10441 aaatttttcg cttggaataa aatcaagccc gcaaagagaa acaagcgtgg ctaaatttcc
    10501 gccgaacaaa acaccttttg cttcgccttc aaagtaaact tttgcctctt tgcgctcggg
    10561 taagagcata agttccccaa cattttgcaa agtttcaaaa aaagattttt ctgtaacttt
    10621 tgaaacttct tcaaccccga aatcactttg cgccatagga gaataaaaag ttatgagtcc
    10681 tgtttttttc aaaatcattg cctgaagcgc acttgtatcg gaatagcctg caaaaatttt
    10741 gggattgttt ttgattaatt caaaatcgat tttatctaat agtcttattg tgccatagcc
    10801 gcctcttgcg caaagaattg cgtttatatt tttgtcctta aaaaatctgt gtaattcttt
    10861 aattttatct tcgtcttttc ctgcaagata gcggttttta tcgaaaatat ttttagaaaa
    10921 aacaaccttg tacccttttt tttcaaagta ttcttttgcc ctgtaaatgt tttcttcttt
    10981 ttcaacagtg cctgaaagtg ctataaaacc tattgtatcg ccctttttta atttttcagg
    11041 tttaattaaa ttcataattg tttaaatttt ccttttacta agctataata atcttattgg
    11101 taaaattaaa aattgcaagt gagggaaata cccctcatcc ggctttcagc caccttctcc
    11161 cgcaagggga gaagggtcgc cgaaaccccc tcgccccttg agggagaggg ttggggtgag
    11221 gggtaaaatt caaaaatctt gcaattatag aatcaaaagg ataaaatgag cgaaaagtac
    11281 gttcctttat acagaaaata ccgtccgcag acgctggaag aaattgtagg tcaagagcac
    11341 attaaaaaat ctcttgcaaa tgcaataact ttgaacaaaa tttcgcacgc ttatttgttc
    11401 acaggaccaa ggggtacggg gaaaacttct accgcaagaa tccttgccaa atcgcttaac
    11461 tgtattcaag ggcagagcgt tactccttgc ggattgtgcc ccagctgtat tgaaattaca
    11521 aactcaattc ctgttgatgt tattgaaatt gacgctgcaa gcaacagaaa agtcgaagac
    11581 gccagaaaca tcttagaaaa aatccagtac gtaccggtaa acggcagata taaaatttat
    11641 atagtagatg aagtccatat gcttacaaaa gaggcgttta acaccctttt aaaaacactt
    11701 gaagaaccac cggaaaatgt gatttttatt cttgcaacga cagagcccca caaagtttta
    11761 gaaacaatta ccagccgctg ccagaggttt gatttcagaa gaattaccac agacgacata
    11821 attgcttatt taaaagaaat tgcccaaaag gaaaaaataa atattgataa cgatgctctt
    11881 tttaccatcg ccaaaaactc tgcaggcgga atgagagatt ctttagccct tttagaccaa
    11941 gttagtgttt tgggagattc tcaaaaaatt aacgcagaag acgttaataa tcttttggga
    12001 agactttctt ttgatttgtt gcataagctt tctaataata taattaattc agaaacgcaa
    12061 gaagcaatag agtgcttgga gagaatttat aactcgggaa acgagcctgt tcaaattatg
    12121 ctaaatttgt tgggttattt caaaaatctt cttatcgtaa aaaattgtac ggataaaacg
    12181 ttgttaattg atttgaccca acttaacgat tctcaaataa aaatccttca agaacagatg
    12241 caaaaagcag aaattcatca aatagttttt cttatcgaaa gaatttctta ttatattaaa
    12301 gaacttaaga ccacaaatgc tcagcatctt tggcttgaag tcgcaatgat agatttggcg
    12361 aatatggctc aaaactcttc gctgattgct cttcaagaaa gactttcaag gcttgaaaca
    12421 agcggtgttt ctgccaaccc tcagcaatac actacacaac ctgctccgat tccgacagag
    12481 ccaataaaat cggttacgga aaacgccaaa caaacagaat cggtaaaaaa agatgaaaaa
    12541 ataagcgttg aaaaagttat tcaaaaaatt gaagaaaaac ttgaaataaa agatttttcg
    12601 cctatgccta agactgagcc caagccacag ccaaaacaac aaaataaaga caattttatg
    12661 gaaagctgga atcaattttt acttttgctt aagcaaaaaa gccctccgac ttacgccatg
    12721 gtaagccaac attccattcc tgtggaattt tctccccaaa aagtcaccat agcctgcaga
    12781 aacagtctgc ttttgaattc ggatgaaaag aaaaaatcca ttaaaaatgt tgccgaagaa
    12841 ttttttagtt cgcaagatat aagcgttgtc ataagacttc aatctgacaa tgacaaaata
    12901 attgaaccga ctaccgctcc ggttgaaaaa aaaaagctga atgaaacagc acaaactcag
    12961 tcggaaaatt tgcccgaaga agaaattcaa gaagaaaatt tagaagaaat tgtttcaaca
    13021 gaaaatttgc ctgaaaaaac aacccaaaaa gttatgatgt ctgatcaggt aaatatggtt
    13081 atggggcttt ttgacggcaa atatattgat tagttaggat tataaatccc tacaaatttt
    13141 acccgatacc a
//
LOCUS       DAOQAU010000046        12889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5367_length_12889_cov_6.422394, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000046 DAOQAU010000000
VERSION     DAOQAU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_12889_cov_6.422394"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1736)
                     /locus_tag="PKI94_06860"
     CDS             complement(393..1736)
                     /locus_tag="PKI94_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNW26491.1"
                     /translation="MMNKLKFYLSILVAKILAVFIAMLHRSSGTSFVGLIALKICPEF
                     LSYCSKYVKKGVITITGTNGKTTTAGLISSILECGGQKVIHNEKGANMLTGIANALAL
                     DIAPFRRFDYFVLESDEAYLTRLYDYLPSNSLLVTNLFRDQLDRYGELDTTAKKIREA
                     IDKNPDLKLFLNADDPMVANLPDEHERIYFGFENVEIVSNSVVSNAPIEAVNCPTCFS
                     TLKYSQRFFAQQGHYYCDCGYKRSYCDYSADVKIYEDYSVINVYSKNGEYEFNVGLIG
                     LYNAYNALAAISFALESGYDQPKIQNALNSYQAIFGRAEKLILQGHQLLVQLIKNPTG
                     ASEVLKTVDLNSNIMVIINDNYADGRDVSWLWDADFEYLKDAKNPIIVSGFRAQDMAV
                     RLKYAGIEPSKIIVIPRIRHALRHVLTTLDINERITILPTYTALLKMQNIIRDFN"
     gene            1874..2233
                     /locus_tag="PKI94_06865"
     CDS             1874..2233
                     /locus_tag="PKI94_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26492.1"
                     /translation="MKIFFVTFFIAELIIALAVIFKIRQLNKAVNKFNKTVTKNKYKI
                     ASKIEDFRYLCVDINRKIEETKEYIRQKRDEYLIKASKTILIYLGIFLLKGKYRKTIL
                     GYQLIKEVYAGLCETEV"
     gene            2293..2604
                     /locus_tag="PKI94_06870"
     CDS             2293..2604
                     /locus_tag="PKI94_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNW26493.1"
                     /translation="MGKENNSISFGLGLIAGVVGGIIAGVLYAPKPGEESRKELKNTV
                     DDLVEKHSPEIQDAKKQAMESIDLMKYKLERQYRKLNHVFKSKKMQKAKELEDIDYDF
                     N"
     gene            2702..3085
                     /locus_tag="PKI94_06875"
     CDS             2702..3085
                     /locus_tag="PKI94_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HNW26494.1"
                     /translation="METSQIALNNGITFLVIATGIIIIIVGGFLVKLLFDMSKLTKNI
                     DETTTIVKTEIEPTLKEFNKALQSINSIAQNADKKVDSLAHTVESLLNTGAFALHKAK
                     DLSGGLIKGMVKGLVSVIKMFVSKR"
     gene            3121..3402
                     /locus_tag="PKI94_06880"
     CDS             3121..3402
                     /locus_tag="PKI94_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNW26495.1"
                     /translation="MSAGKFLAGFVVGGVLGAVAGVLLAPQSGEETRELLSDASKDMV
                     KKTDKTVKEIQQKADIAVSDMQKKGDEIMEKIQTLINKQKGEKPAEETN"
     gene            3635..3916
                     /gene="gatC"
                     /locus_tag="PKI94_06885"
     CDS             3635..3916
                     /gene="gatC"
                     /locus_tag="PKI94_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNW26496.1"
                     /translation="MITVKDVEHVAKLARLELTQEEKEKFTKQLGDVLKYVEQMNEVD
                     TTDVKPMAHAFDIVNVMRDDNIVYEQTKEELMANAPEEENGFFKVPKIG"
     gene            4009..5655
                     /locus_tag="PKI94_06890"
     CDS             4009..5655
                     /locus_tag="PKI94_06890"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNW26497.1"
                     /translation="MGTKYIFITGGVVSSLGKGIIGASLGKLLKARGYSVAIQKFDPY
                     INVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTDTSLSQINNVTSGSVYQTVISK
                     ERKGDYLGATVQTIPHITNEIKSRIVKAKNQFKTDFLIIEIGGTIGDIEGLPFIEAIR
                     QFKTEVGFGNAISIHCTLLPYLPTSGELKTKPSQHSVTTLRSYGLQPEVLVCRTTRSI
                     PKTEKEKLALFCSVPKEAVIECKDMKSIYEVPLALEEQGMASVVLQRLMMEDRKPELK
                     SWKNLVEKIKNPKMTIKVAIAGKYTKLSDAYISVVESLKHAGYANDAQIEIKWINSED
                     CIDYDKANELLKDVQAVVVPGGFGIRGIEGKLNVIKYARENNLPFLGLCLGMQCAVIE
                     YARNVLGFADANSTEFDENTKYPVIDLMLEQKNVQGYGGTMRLGQYECHIKKGSKANS
                     AYGQTKIFERHRHRYEVNSEYMEQFKKAGLIFSGLSPDGMLAEIVELPQLDWFVASQF
                     HPEFKSRPEKPHPLFDGLISTAKNKIENLNKIKKLEKTVR"
     gene            complement(5659..5832)
                     /locus_tag="PKI94_06895"
     CDS             complement(5659..5832)
                     /locus_tag="PKI94_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26498.1"
                     /translation="MTKFERCDAKCLLQKAGRLPDGIKISKNPVKNFAYFMKNFINNY
                     KLIRKSIKARIGK"
     gene            complement(5991..7391)
                     /locus_tag="PKI94_06900"
     CDS             complement(5991..7391)
                     /locus_tag="PKI94_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26499.1"
                     /translation="MSVNLAEQFEENEQYDKAYEEYKKAYAQRPKSIEVLERLGHVAI
                     ILDKKDEAQEYYQKILELDMANALAYEQLMDIYVHTDRYKYYVSRGNLHVIQQELSHA
                     IGDFKKAIEKAQDQAQSNSARFVLASLYEQVGKNHNAIDEYLRILDTQGANEFVYLKL
                     ADIYFKEDAISSAIEILQRALDCGFDSDAVKESLARLYLKDNQPDKALEFTQDDLVKV
                     KSLLENEKNDEAFKILEKIKPSHTKNFQYFLLLAQYYFNTKNFEKSLEGVNEFSKLEP
                     DSPLAYQMRAMIFEEQKNEFDAHINWAKYNLAKKDWDVALNEYFHAFQIKDKDVTLIK
                     NIAELLDDMKDSTHAAEFWEKLAALEPNNKKALQRLADFNESIGDYKTQIKFLEKLYE
                     IDTKNSVVIKTLAKAYEKTKNKEKSLEFYNKFIEVAPVGDDYEQIKQKLAKLEQTDFR
                     EEEGLIDKIMRLFINK"
     gene            7607..10369
                     /locus_tag="PKI94_06905"
     CDS             7607..10369
                     /locus_tag="PKI94_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26500.1"
                     /translation="MALSWNEIKDRALKFSNEFKDARYEKGETQSFYNEFFNVFGINR
                     RRVATFEEPVKKLGDKQGFIDLFWKGVMLVEQKSQGRDLTKAKTQALEYFPNLKDEEL
                     PRYILLSDFQTFELFDLETNDEHKFDLKDLYQNVGLFDFIAGYEKKSYADTETVNIEA
                     SELMGKLYDELKISGYPQHDLELFLTRALFCLFADDSDIWERNLLTQYIEERTAKDGS
                     DLGLKISLLFQILNTPQEKRSANLDEHLKRFAYINGGLFALHVEIPSFNSKMREVFLE
                     CCRYDWRKVSPAIFGSLFQSVSNPKKRRELGEHYTSEQNILKLIEPLFLDELWEEFEK
                     VKHNRNRLEEFHNKVASLKFLDPACGCGNFLVVTYRELRRLETQVILALDSFKKDSGQ
                     MRIDVASLSKLDVDNFYGIEIEEFPCKIAEVALWMTDHLMNMELSTLLGSFYTRIPLV
                     KSAKIVNANSLSLDWNEVVKPEELNYILGNPPFIGKQMQSKDQSAEMASIFAGVKGAG
                     VLDYVTAWYLKSAQYIKNYSHIKCAFVSTNSITQGEQVGILWNELLKNYNIKIHFAHR
                     TFDWCNEARGNAHVHCVIIGFANFDSNKKRLFDYEDIKGEPHETEVKNINPYLIDCDD
                     LVVLKRSKPLLDTPEIVFGNMANDGGNLIFNNEEEKNEFIKQEPLAEKYIKTFIGAEE
                     FINNKVRYCLWLKEVNPMELAKMPMVLDRINKVKVLRSQSTRKETRELANTPALFGEI
                     RQPNCDYIVIPRVSSERRPYIPFGFINKDLIVSDRCTIIASSDLSLFGILSSSMHITW
                     TKYTCGRMKSDYNYSNTIVYNNFPFPKDILDKNKQNVEQKAQAVLDVRAKYQNTDCGE
                     SCSLAVLYNPETMPPDLMKAHQELDRAVDKCYGKTSFKSERERIEFLFSLYEQYTKPL
                     LTKLSG"
     gene            complement(10389..10943)
                     /gene="pth"
                     /locus_tag="PKI94_06910"
     CDS             complement(10389..10943)
                     /gene="pth"
                     /locus_tag="PKI94_06910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNW26501.1"
                     /translation="MKLIAGLGNFEPKHLLTRHNAGFMAVDFLLQRHAQTFKVEQKLK
                     SKIAKFKLNGEDVVVIQPLTYMNLSGEALVAVMNFYKIDVKDILVIYDDISLDLGTLR
                     FRADGSDGGHNGIKSIIKLLGTKEFDRLKIGIGPQPNIPSESFVLQNFTQDELVKIKE
                     ILKRPVIEDYLQKGLEQAQNLYNR"
     gene            11112..11681
                     /locus_tag="PKI94_06915"
     CDS             11112..11681
                     /locus_tag="PKI94_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26502.1"
                     /translation="MTTKNKPIIQTKSSQKVAKFLDSNSLHKKDFAEMIGVTLSYVYN
                     LIDNTIPFSTRSTTLERIATVMEIEPEEFEEYKIPQEPILIDDSIEFLKDSLKLKGLT
                     TVNFLKSFPRKKRLDIVDLLRGSSPLPIDFKELMMIAQVLDLKKDEIYPLWENRMRQV
                     LESSGMNVNANSALVNTMFDCAKKFVNLK"
     gene            11955..12030
                     /locus_tag="PKI94_06920"
     tRNA            11955..12030
                     /locus_tag="PKI94_06920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11988..11990,aa:Lys,seq:ttt)
     gene            complement(12374..12772)
                     /locus_tag="PKI94_06925"
     CDS             complement(12374..12772)
                     /locus_tag="PKI94_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26503.1"
                     /translation="MLISKTNFADIKRNNKPGFGMALHMDMGKIAKTIGKEAAGSIEK
                     ATPSLEALAQDVDVFIEPVVGNRLSIKVQKLTEPIIKSKNPILNFFRAIKRQIQLFNK
                     DFAREEISLGNNINLKILKETSYLKELLQK"
BASE COUNT         4314 a         2234 c         2310 g         4031 t
ORIGIN      
        1 atgattatgg tactttacta ccacgagaac atgaccttaa aagaaatcgg agaaaccatt
       61 gaggtttcag aatcaagggt ttgccagctt catgcacagg caattatgaa gttgaagaac
      121 atcttgagcg aaaaccgcac agaaagaatg aaaaagagta tagtttagag ttatttttat
      181 ttaattttat aattaatttt ttacttgcta atattttttc atgtcgttgc gaggaagaaa
      241 tttatttctg acgaagcaat ctaaaaaacg ttaagcatta aatatttttt tgctctacgt
      301 acttagtttg tagggtgcgt cacatgcacc tttttattct atttcctatt tccttgattc
      361 aggatgacgt atttttttta tctcaacaaa ctttaattaa aatccctgat tatattctgc
      421 atttttaaaa gcgcggtata tgtgggaaga attgttatac gctcatttat gtcgagcgtc
      481 gtcaaaacat gccttagagc atgccgtatt cttggtataa caataatttt agaaggctca
      541 atccctgcat acttaagcct tacagccatg tcttgcgctc taaagcccga tactataatc
      601 ggatttttag catcttttaa gtattcaaaa tccgcatccc aaagccatga aacatctctg
      661 ccgtcagcgt aattatcatt aattataacc ataatgtttg aatttaaatc gactgttttt
      721 aatacttcgc ttgcacctgt gggatttttg attaactgca ccaaaagttg atgaccctgc
      781 aggataagtt tttcggcacg accgaaaatt gcctgataag aattcaaagc attttgtatt
      841 ttaggctgat cgtatccgga ttcaagagca aaagagattg ctgcaagtgc gttatatgca
      901 ttgtataaac cgattagacc gacattaaat tcatattccc cgtttttaga ataaacgtta
      961 ataaccgaat aatcttcata aattttgaca tcggcactgt agtcacaata ggaccgtttg
     1021 tagccgcaat cacagtagta atggccctgt tgggcaaaaa atctttgact gtattttaaa
     1081 gttgaaaaac aagtaggaca gtttaccgct tcaattggag catttgacac aacagagttt
     1141 gaaacgattt caacattttc aaagccgaaa tatattcttt cgtgttcatc gggtaaattt
     1201 gctaccatag gatcatccgc atttaaaaat aatttcaaat caggattttt gtctatggct
     1261 tctctgattt tttttgcggt agtatcaagt tcaccgtaac ggtcaagttg atccctgaaa
     1321 agatttgtaa ccaaaaggct gtttgacggc agataatcat aaagtcttgt cagatatgct
     1381 tcatcggatt caagcacaaa ataatcaaaa cgacgaaacg gtgctatgtc aagggcaagc
     1441 gcgtttgcaa tgccggttaa catattcgct cctttttcgt tatgaataac tttttgtccc
     1501 ccacattcca aaatacttga aatcaaccct gccgtcgtag ttttaccgtt tgttccggta
     1561 atggttataa cacctttttt gacatatttt gaacagtatg acaaaaactc gggacagatt
     1621 ttaagtgcga taagcccgac aaacgaagtt cccgaagatc tgtgcaacat tgctataaat
     1681 accgccagta tctttgcaac cagtatggat aagtaaaatt tcagcttatt catcattcat
     1741 catttaccat ttatcattta ctatttacta tttactattt actatttact atttaaatag
     1801 tcttcaaggt gtattcaaaa taattaaaat ataccttata attatcatca taatacaaca
     1861 acagattaaa atcatgaaaa tattttttgt aacatttttc atagcagaac ttatcattgc
     1921 ccttgccgta atatttaaaa taaggcagtt aaataaagct gtaaataaat tcaataaaac
     1981 ggttacaaaa aacaaatata aaattgcttc caaaatagaa gatttcaggt atttatgcgt
     2041 tgacataaac cgaaaaatcg aagaaacaaa agagtacatt cgccaaaaac gcgatgaata
     2101 tctgataaaa gcatcaaaaa caatcttaat ctatcttgga atatttttat tgaagggaaa
     2161 atatcgcaaa accattctcg gatatcagct tataaaagag gtgtatgcgg gactttgtga
     2221 aacggaagtt tgattgtttt tttgaaaaaa tatgttatac ttgataaaaa taaggatgaa
     2281 aggacacaga aaatgggtaa agaaaacaat tccatcagtt tcggactggg acttattgca
     2341 ggcgtcgtgg gcggaataat tgcaggggtt ttatacgcgc caaaaccggg tgaagaatca
     2401 agaaaagaac ttaaaaatac cgttgacgat cttgtagaaa aacattcgcc tgaaatccag
     2461 gatgcaaaaa aacaggcgat ggaatccata gaccttatga aatacaagct cgaaaggcaa
     2521 tatagaaaac ttaatcacgt tttcaaatca aaaaaaatgc aaaaagcaaa agaacttgaa
     2581 gacattgatt atgattttaa ttaagtaagt cgttggcgaa gggatatgcc ctgagcctta
     2641 cgaattactt atcccgaact tgattcggga tctcgtacgc aaagattaat gaaaggatga
     2701 aatggaaaca tcgcaaatag cactgaataa cggcataact tttttggtta tcgcaacagg
     2761 aattattatt ataatcgtgg gcggattttt ggtaaaactt ctgtttgaca tgtcaaaact
     2821 tacaaaaaat attgacgaaa caaccactat agtgaaaaca gaaattgagc cgactttgaa
     2881 agaatttaac aaagcgcttc aaagcataaa ttccattgcc caaaacgcgg acaaaaaagt
     2941 cgacagcctt gcacacaccg ttgaaagcct tttaaataca ggtgctttcg ctcttcacaa
     3001 agcaaaagat ttatccggcg gtttaatcaa aggaatggtc aaaggacttg tatcagtaat
     3061 aaaaatgttt gtaagtaaac gataatatta gcaaaatata aatagtaaag gagaaatatt
     3121 atgtcagcag gtaaattttt agcaggtttt gtagtaggcg gagttctcgg cgctgttgcg
     3181 ggagttcttc ttgcccctca atcaggtgaa gagacaagag aattgctctc tgatgcttca
     3241 aaagatatgg tcaaaaaaac agataaaaca gtaaaagaaa ttcagcaaaa agcagatatt
     3301 gccgtttccg atatgcaaaa aaagggcgat gaaattatgg aaaaaattca aacgctcata
     3361 aataaacaaa aaggtgaaaa accggcagaa gaaactaatt aattttttaa tttaaaaaaa
     3421 agagggcgaa tttttgtgaa acaaaaattc gccctctttt gtccggctca tacccatacc
     3481 gccggctatc ccgcagccgg taagggtatg agcacttctt aagtcagatt tgaaaaattg
     3541 cgaaaaattg tcatttttcg aatttttccg catcctcttt tgcaaataat tgtttcatga
     3601 tatcattata gaataatttg catggagtta aaaaatgatt acggttaaag atgttgagca
     3661 tgtagcaaaa cttgccagat tagaattaac acaagaagaa aaagaaaaat ttaccaaaca
     3721 actcggcgat gtgcttaagt atgttgagca aatgaatgaa gttgacacca cagatgtcaa
     3781 gccgatggcg catgcatttg atattgtaaa cgtaatgaga gatgacaata ttgtctatga
     3841 acagacaaaa gaagaattaa tggcaaacgc gcctgaagaa gaaaacggat ttttcaaagt
     3901 acctaagata gggtaaatag gaaataggaa atagtgaata gatttctaaa ctttctattt
     3961 accatttact atttaccttt tacttcatta aaaaatgagg agtgaataat ggggactaag
     4021 tacatattta taaccggtgg agttgtaagc tctttaggaa aaggaattat cggagcttct
     4081 ttaggaaaac ttcttaaagc aaggggctat tccgttgcga tacaaaaatt tgacccttat
     4141 ataaacgttg accccggcac aatgagtccg tttcaacacg gcgaagtttt tgtaaccgat
     4201 gacggtgcag aaaccgattt ggacttgggg cattacgaaa ggtttacgga taccagcctg
     4261 agccaaataa acaatgttac atcaggaagc gtttaccaga ccgtaatttc gaaagaaaga
     4321 aaaggcgatt atctgggtgc gacggttcag acgattcctc acataacaaa cgaaattaaa
     4381 tcaagaattg taaaagctaa aaaccagttt aaaacagact ttttaataat agaaataggc
     4441 ggaacaatag gtgacataga aggtttgccg tttattgagg caataagaca gtttaaaaca
     4501 gaagtcggat ttggaaatgc aatttcaatt cactgtacgc ttttgcctta tttgcccact
     4561 tcgggtgaat taaagacaaa accctctcaa cacagtgtta caaccttgag aagttacgga
     4621 cttcaacctg aggttttggt ttgcagaaca acccgttcaa ttccaaaaac agaaaaagaa
     4681 aaacttgcgc ttttctgctc ggttccaaaa gaagcggtca ttgaatgcaa agacatgaaa
     4741 agcatttacg aagttccgct tgcattagaa gagcagggta tggcgtctgt tgtgcttcaa
     4801 agacttatga tggaagacag aaagcccgaa ttgaaatcat ggaaaaatct tgttgaaaaa
     4861 attaaaaacc ctaaaatgac aataaaagtt gcgattgccg gaaaatacac aaaactttca
     4921 gacgcctata tttctgttgt tgaatcccta aaacatgcag gctatgcaaa tgatgctcag
     4981 attgaaatta agtggattaa ctccgaagac tgcattgatt acgataaggc caatgaactt
     5041 ttaaaagatg ttcaggcggt agttgtcccg ggagggttcg gaattcgcgg aattgaaggc
     5101 aagctcaatg ttataaaata tgcgcgtgaa aacaatttac cgtttttagg gctttgcttg
     5161 ggcatgcagt gtgcggtaat tgaatacgca agaaatgtct taggatttgc cgatgccaat
     5221 tctacagaat ttgacgaaaa cacaaaatat cctgtcatag atttaatgct tgaacaaaaa
     5281 aatgttcaag gatatggtgg cacaatgagg cttggtcaat atgaatgcca catcaaaaaa
     5341 ggctctaagg caaattctgc atacggtcag acaaaaatat ttgaacgcca tcgacacaga
     5401 tacgaagtca atagtgaata tatggaacaa tttaaaaaag cggggcttat attttcggga
     5461 ctttcacccg acggaatgct tgcagaaatt gttgagcttc cccagcttga ttggtttgtg
     5521 gcatcacaat ttcatcccga attcaaatca agaccggaaa agcctcatcc gcttttcgat
     5581 ggtttaattt ccaccgcaaa aaataaaata gaaaatttaa acaaaatcaa aaaattagaa
     5641 aagacagtaa gataaaattt atttgcctat tcttgctttt attgattttc tgattaattt
     5701 atagttatta ataaaatttt tcataaaata tgcaaaattc ttaaccggat tttttgagat
     5761 ttttatcccg tcaggaagcc tgcccgcttt ctgcaataaa cactttgcat cgcagcgttc
     5821 aaatttggtc attgtgtaaa tggtatgtaa atcaactcta tttttgaata taatcataaa
     5881 tccaacataa aaatgtatat atatatttaa aaacaacaat caatataaat ttttgtccaa
     5941 agatacttgt cttttcataa aaatttacaa taaataaaat gccacaaatt ttatttattg
     6001 ataaaaagcc tcataatttt atcaatcaaa ccttcttctt ctctaaaatc agtttgttca
     6061 agctttgcaa gtttttgttt aatttgctcg taatcatctc cgacgggtgc aacttcaatg
     6121 aatttattgt aaaattcgag agatttttct ttattttttg ttttttcgta agcttttgca
     6181 agggttttta ttacaactga gttttttgta tcgatttcgt acaatttttc caaaaattta
     6241 atttgggttt tgtaatcgcc tatgctttcg ttaaaatcgg caagccgctg aagtgctttt
     6301 ttattattgg gctcaagtgc tgcaagcttt tcccaaaatt cagctgcgtg tgtagaatcc
     6361 ttcatatcgt ctaaaagttc tgcgatattt tttattaaag ttacatcttt atctttgatt
     6421 tgaaaggcat gaaaatattc attaagcgca acatcccaat cttttttggc aagattatat
     6481 tttgcccagt tgatatgtgc atcaaattca tttttttgct cttcaaaaat catagcacgc
     6541 atttggtacg caagcggcga atccggttca agcttgctaa actcatttac cccctcaagg
     6601 cttttttcaa agttttttgt gttgaaatag tactgcgcta aaagcaaaaa atattgaaaa
     6661 ttttttgtat gcgaaggttt aattttttct aaaatcttaa aggcttcatc gtttttttcg
     6721 ttttcaagaa gactttttac tttaaccaaa tcgtcttgag taaattccaa cgctttatcg
     6781 ggttgattgt cttttaaata cagacgggca agactttctt ttaccgcatc ggaatcaaat
     6841 ccgcaatcca aagctctttg cagaatttca atcgcacttg aaatagcatc ttccttaaaa
     6901 taaatatcag caagtttgag atagacaaat tcgtttgcgc cttgtgtgtc caaaattctt
     6961 aaatattcat caatggcatt gtggtttttg ccgacctgtt catagaggct tgcaaggaca
     7021 aagcgcgcag aattgctctg ggcttggtct tgcgcctttt cgattgcctt tttaaaatcg
     7081 cctattgcat ggcttaattc ctgctgaatt acatgcaggt ttcctcttga aacataatat
     7141 ttgtagcggt ctgtgtgaac gtaaatatcc ataagctgct catatgcaag agcatttgcc
     7201 atatccagtt caagaatttt ttggtaatat tcctgcgctt cgtctttttt gtccaaaatt
     7261 atcgccacat gccctagacg ctcaagcact tcaatacttt ttggtctttg ggcgtaagct
     7321 tttttgtatt cttcgtaggc tttgtcgtac tgttcgtttt cttcaaactg ctcggctaaa
     7381 tttacactca ttttgtaact tctcctcaaa acgtcatgct gaatttattt cagcatctat
     7441 cctatatact ttggtggata gatcccgaaa caagttcggg atgacaaaaa tataatgtaa
     7501 aacaattata aatattaaac caaaaacaca aatttaaaac cagtcacaag gctattattt
     7561 tttgatttat gttataattt tttatgaaat caactttagg agatttatgg cattaagttg
     7621 gaacgaaatc aaagacagag cgttaaaatt ctctaatgaa tttaaagatg ctcgatatga
     7681 aaaaggtgaa acacaatcct tttacaatga attttttaat gttttcggaa ttaatagaag
     7741 gcgtgttgcg acttttgaag aacctgttaa aaaacttggc gacaaacaag gctttattga
     7801 tttgttttgg aagggcgtta tgcttgtcga gcagaaatcg caaggcaggg atttaacaaa
     7861 agccaaaact caagcgttgg aatattttcc gaacttaaaa gatgaagaat tacctagata
     7921 tattttactt tctgattttc agacatttga attatttgac cttgaaacga atgacgaaca
     7981 taaattcgac ttaaaagatt tgtatcaaaa tgtcggatta ttcgatttta ttgcagggta
     8041 tgaaaagaaa tcttatgcag atactgaaac tgtcaatatt gaagcctcag agcttatggg
     8101 taagctatat gatgaattaa aaatttcagg ttatccgcaa cacgacttgg aactattttt
     8161 gacaagagcg ttattctgtt tgtttgcgga cgattcagac atttgggaac gtaatttatt
     8221 aactcaatat atcgaagaaa gaacagccaa agacggctct gatttgggct taaaaatatc
     8281 tttgttgttt cagatattaa acactccaca agaaaagcgt tctgctaact tggatgaaca
     8341 cttaaaacga tttgcatata tcaacggcgg tttgtttgct ttacacgttg aaattccgtc
     8401 tttcaattcc aaaatgagag aagtattttt agaatgttgt cgttatgatt ggcgaaaagt
     8461 ttcccctgcg atatttggtt ctttgtttca atcggtatct aatccgaaaa aaaggagaga
     8521 acttggcgaa cattacacct ctgaacagaa cattcttaaa ctgattgagc ctttgttttt
     8581 ggatgaactt tgggaagaat ttgaaaaggt taaacataat aggaacagac ttgaggagtt
     8641 tcataataag gttgcaagcc taaagtttct tgaccctgct tgcggttgcg gaaacttttt
     8701 ggttgtaact tatagagagt tgagaaggct tgaaacgcaa gttatattgg cattggattc
     8761 tttcaaaaaa gattcaggtc aaatgagaat tgacgttgct tcactttcaa aacttgatgt
     8821 cgataatttc tatgggatag agattgaaga attcccttgt aagattgcag aagtagcgct
     8881 ttggatgaca gaccacttga tgaatatgga actgtcaacc ctattaggct ctttttacac
     8941 aaggattccg cttgtaaaat cggcaaagat tgtcaacgca aattcactct ctcttgattg
     9001 gaacgaagtc gttaagcccg aagaacttaa ctatatctta ggcaatccgc catttatagg
     9061 gaaacaaatg caatctaaag accaatctgc ggaaatggca agtatatttg caggagtgaa
     9121 aggtgcaggg gttcttgatt atgttactgc atggtatctg aaatcggctc aatatatcaa
     9181 aaactattct catatcaaat gtgcttttgt ttcaacaaat tcgattactc aaggcgaaca
     9241 agtcggtatt ttgtggaacg agcttttgaa aaactataat atcaaaatcc actttgcaca
     9301 cagaactttt gactggtgca atgaagcaag agggaacgcc cacgttcatt gtgtaattat
     9361 cggctttgca aattttgact caaacaagaa acgacttttt gactacgaag acataaaagg
     9421 cgaaccgcac gaaactgaag taaaaaacat aaatccgtac ttaattgatt gtgatgattt
     9481 ggtagtgtta aaacgttcaa aacctttgtt ggatactccc gaaatagttt ttgggaatat
     9541 ggcaaacgat ggtggcaatt taatctttaa caacgaagaa gaaaaaaatg aatttataaa
     9601 acaagagcca ttagctgaaa aatatatcaa aacatttatt ggagcagaag aatttattaa
     9661 taacaaagta aggtattgtt tgtggttaaa agaggtcaac cctatggaat tggcaaaaat
     9721 gccaatggtg cttgatagaa taaataaagt taaggttcta cgttcacaaa gtacaagaaa
     9781 ggaaacaaga gagcttgcca atactcctgc actttttggc gaaattaggc aacctaactg
     9841 tgattatatt gttattccaa gagtttcatc cgaacgaaga ccttacatcc catttggatt
     9901 tataaacaaa gaccttattg taagtgatag atgtacgatt attgcaagta gtgatttgtc
     9961 tttatttgga atattgtctt ctagtatgca cattacatgg acaaaataca cctgcggcag
    10021 aatgaaaagt gattacaact attcaaatac tattgtttat aacaattttc cattcccgaa
    10081 agatatatta gataagaaca aacagaatgt tgaacaaaag gctcaagcgg tgcttgatgt
    10141 tagggcaaaa tatcaaaata cggattgtgg tgaaagttgc tcacttgcgg ttttgtataa
    10201 ccccgaaaca atgcctcctg acttgatgaa agcacatcaa gaactagaca gagccgttga
    10261 caaatgctat ggcaaaacat ctttcaaatc cgaacgtgaa agaatcgaat ttctgttctc
    10321 tctctacgaa caatacacaa aaccattgtt gacaaaacta tcaggatagt aatcctgacc
    10381 tacaaacttt atcgattata caaattttga gcctgctcaa gtcctttttg taagtaatct
    10441 tcgattacag gccgttttaa aatttcttta attttgacca attcgtcctg ggtaaaattc
    10501 tgcaaaacaa aactttctga aggaatattg ggctgaggcc ctattccgat tttaagtctg
    10561 tcaaattctt ttgtccccag caattttatt atcgatttaa ttccattgtg tccgccgtca
    10621 gatccgtcgg cgcggaatcg caatgtacct aaatcaagcg aaatatcatc ataaattacc
    10681 aaaatatctt tcacatcgat tttgtaaaaa ttcatcactg caacaagcgc ctctcccgac
    10741 aaattcatgt aagtcaaagg ctgaattacc acaacatctt caccgtttaa tttaaatttt
    10801 gcaatttttg atttgagttt ttgctcaact ttaaaagttt gggcatgcct ttgcaataaa
    10861 aaatctaccg ccataaaacc ggcattgtgc cttgttaaaa ggtgtttcgg ttcaaagtta
    10921 cctaaccctg ctatcagttt catttcttct gttgtccttt ttgaaagatt atacttataa
    10981 ttttaaccca aataaatatt accatgatga tttatctgac ttgtgcatgt taaaaaagtt
    11041 aaaaaagttt atgaatataa tacaaataat atgaattcat ttatgaactt catacaagag
    11101 aggggatgat tatgacgaca aaaaataagc cgattattca aacgaaaagc tcgcaaaaag
    11161 tggcaaaatt tttggacagc aattctttgc acaaaaaaga cttcgcggaa atgatcgggg
    11221 taacactttc ctacgtttat aatcttatag acaatacaat tccgttttca acacgctcta
    11281 caactctgga acgcattgca acggttatgg aaattgaacc cgaagaattt gaagaatata
    11341 aaattccgca agaacccatt ttgattgatg attcaataga atttttaaaa gacagcctta
    11401 aattaaaagg gcttacaaca gttaactttc ttaaatcttt cccgcgcaaa aaacgacttg
    11461 acattgtgga tttgctcaga ggttcaagcc ctttgccgat tgactttaag gaacttatga
    11521 tgattgctca agttctcgat ttaaaaaaag acgaaattta tccgctgtgg gaaaacagaa
    11581 tgagacaagt gcttgaatca agcggaatga atgttaatgc aaattctgcg ctggtaaaca
    11641 cgatgtttga ctgtgccaaa aaatttgtta atctgaagta aaaatgttcg aaaaagtgtg
    11701 taaaccaaag cggcggggtg ctaagcaccc cgccgcttca ttttattcaa aaaacagttg
    11761 actgaaaaac atgttcttga taggctttta gtaaggatta acaatataca ctagtctgtt
    11821 aaacttaatt tccgtagcga tacgggtgac aagtaaataa tgtaggaagt aacaaaagac
    11881 cacaatctgt tttgcacacg caataaaccc tatgtgaaaa gtctttttgc ttactttttc
    11941 tacagaaaaa gtaagcgcca ctagctcagt tggtagagca ctcccctttt aagggatagg
    12001 tcccgcgttc gaatcgcggg tggcgtacca tttttaaaga gagaactttc gttctctctt
    12061 tttgtttgaa aaattaatgt cccgcgatac gaccgcagtt gttttttcga catagtgtct
    12121 caaaacaaca ggcgttaatc gacaagtcga taccctcatc tgcaaggctc aaacgtcctg
    12181 ttttgtcttg gattattcgc cgttcggaaa aacgtggttt tcctcacttg gacgagtttc
    12241 gcttcgctca ctcttgcgaa aatccgctcg cctgcatctg agctgaaatc gcgggtggcg
    12301 taccatttta aaagaaggca ttagccttct tttttttatt taccaacttc gcagctcagt
    12361 tggtagagct tctctatttt tgcaacaatt cttttaagta acttgtctct tttaaaatct
    12421 ttaaatttat attatttccc aaagaaattt cttctctggc aaaatctttg ttgaaaagct
    12481 gaatttgtct tttaattgct ctgaaaaaat tcaaaatagg attttttgat ttgataatcg
    12541 gctctgtaag cttttgtact tttatactca agcgattgcc cacgacaggc tcaatgaaaa
    12601 catcaacatc ctgagccaac gcttcaagag aaggggttgc tttttcaata ctgcctgctg
    12661 cttctttccc tattgttttt gcaattttac ccatatccat atgtaatgcc ataccaaaac
    12721 caggcttgtt gttccgctta atatcagcaa aatttgtttt tgaaattaac ataatttatt
    12781 tttctctatt gtgtcctaca ttaatattaa accctctaaa aatatttttt gctagtttga
    12841 caaaatttgt taaaatattt taataaaact ttactcgtac ctctgctcg
//
LOCUS       DAOQAU010000047        12856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5388_length_12856_cov_6.038669, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000047 DAOQAU010000000
VERSION     DAOQAU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5388_length_12856_cov_6.038669"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1323)
                     /gene="dcm"
                     /locus_tag="PKI94_06930"
     CDS             complement(91..1323)
                     /gene="dcm"
                     /locus_tag="PKI94_06930"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNW26504.1"
                     /translation="MSKNVVLKEKRQKNSAKIFPFHIHDWRDKIFSYPEKEIKLATLF
                     SGIGSIEQAMKRLNIKHSIIFAGDIDPHVKTSYFANYNINEEDWHDDITKFSATKYKG
                     LVDLLVGGSPCQAFSMVGKRKGLEDVRGTLFYDFARIVQEVEPKVFIYENVKGLINHD
                     GGKTWQVVQEVFQALGYKINFQILNSKDYGIPQHRERIIVVGFKDTSINFEFPKPIPL
                     EYTMQDFLEDYTETKYYLKEKGVKFVTSSKNRAKRYTQINGTIALCQKANQQFNWHGD
                     FVFENVYHNDFDEFIFDVNKVEEKYYLSDKIRNYVLAGGTKNFKTSDKTDLEIARPLL
                     QTMHKMHRAGVDNYVTHNKGRIRKLTPRECLRLMGFRDDFKIVISDTQMYRQAGNSIV
                     SDVLIAILKQMDITKFGR"
     gene            complement(1441..2739)
                     /locus_tag="PKI94_06935"
     CDS             complement(1441..2739)
                     /locus_tag="PKI94_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26505.1"
                     /translation="MGKIYFIDVTNRDGVQTSRLGLAKLEKTIINLMLDDMGITQSEF
                     GFPTTKHEINYLNGNLELVERGAFKNITLSGWMRAIAEDVELSLKNVPKLKNLNLSQS
                     TSMQMTQGKYQGKKTLDDILKMTCEAVECAVSKGVKLIGVNAEDASRSDLEFLIKYAK
                     EAKSCGAHRFRYCDTLGYEDPKTAYDRIYRIASEAQIDVETHYHNDLGMAVACSVMGA
                     KAAVDAGMNAYINTAINGMGERAGNADLISCLLAVLKSAGFGGKYEIDPKIDLSKAWK
                     LAKYTSYAFGVPIPMNQPAVGDNAFAHESGIHADGALKDRRNYELYDYEELGRGEPEI
                     IETGRMITVGEYGGIKGFRNVYEGLEIEFKDEHEARNVLELARYANVHTQKPLTDSEL
                     KFIYHYPDVAAKIMTVSPYYEPKGALKERLEKDKLKYKSI"
     gene            2843..3550
                     /locus_tag="PKI94_06940"
     CDS             2843..3550
                     /locus_tag="PKI94_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNW26506.1"
                     /translation="MKKIGLLGGLSPESTVEYYKIINSEVRKVLGGCSSAELIIESFN
                     FREIEKMQFLGKWDELGEKLTHAAKNLEKCGADYIVICTNLMHKVAPYVKNRISVPLI
                     HMVDSVAQDIQRQNMNKVGLLGTIFTMEEDFYSKKLLEDYSIETIIPDSKDRQEISRI
                     IYEELCCGIIDENSRKFYLNVIDKMKQNGAQGVILGCTEIPLMIQKASIPIFNTTRIH
                     AKSVVEKILEKEPLLLA"
     gene            3779..4396
                     /locus_tag="PKI94_06945"
     CDS             3779..4396
                     /locus_tag="PKI94_06945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNW26507.1"
                     /translation="MGEVRRNQKIQISFKTNDGTEKIFECKIKELYSDRLMLDYPKDI
                     VFYAEYLSEGEEIYVKIFTPAGIRAYNALILDSPLSENFIIEYVDSNIQIQRRAYARA
                     PLKTKIIIERFDEPNIVTHTVDIGGGGIRFYFDGHFNRNELVNCRLYLPLQMTSVKAQ
                     GHIIEKPHLQTGEHVILFNKINEFDRDKIIKKCFEIEAMKYREYQ"
     gene            4409..6007
                     /locus_tag="PKI94_06950"
     CDS             4409..6007
                     /locus_tag="PKI94_06950"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26508.1"
                     /translation="MKQKIQKEKNIRKTDFKTDNVQKAIEQCQIALQKDPTNADLHVR
                     LGDLYLAWHLDIYNSYQYIDEAITEYQRALETYIDSAEIYFKIGQAFFYKGDLDKAIN
                     YLNMSLKKDAKMAKTYYFLAETYTKKARFADAIYNAKKSIKLKPFSNSCSHFLLHNLY
                     KLSSFKSFKKRLMSKFEFLLSLLTLPFDKAAIKNVIQNLSYLRFIPILMKGYYQIQTK
                     GFTQAVDIYIEAIEKAPGFVPLYCLLGDIYRAIGDFEDAITEYKMAIWLDSLNIPAYI
                     HLCQAYEEQGDYDNAIETYKKLIEIMPHRPEFHSNLANIYFLKGEVDLAVSHYQAAII
                     LNPGKQWTSVIAQTLGFVHQETKEDMDAAISAYQSAYLLTPDDIDIYVNLGSAFYDKE
                     DYDNALTTYRAALELDPVNAKIHCNLGFLHWGKGDTDEAIKEYELAIKYDPTYDVAYN
                     NLGVIYLDDLGRVQQAIELFTKSIEYNPNYALAHFNLARAITIKGDKIEAAKLYQIAQ
                     DINKITNEIDPQEIINKINGLFQS"
     gene            complement(6009..6233)
                     /locus_tag="PKI94_06955"
     CDS             complement(6009..6233)
                     /locus_tag="PKI94_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26509.1"
                     /translation="MPNMYDDFEKRFENAPFFAGWVEFEVKPAKAAPIVKAEHKDIDA
                     LSAELKLIEEQLEEISLKELRLANLAQFYQ"
     gene            complement(6243..6437)
                     /locus_tag="PKI94_06960"
     CDS             complement(6243..6437)
                     /locus_tag="PKI94_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26510.1"
                     /translation="MDKFFNIDEAMIDYSEMTSFNFNSPDYFECCAQKEIPQEYAHQK
                     YTFKFGNFDVVDMLQAVFAK"
     gene            6641..7708
                     /locus_tag="PKI94_06965"
     CDS             6641..7708
                     /locus_tag="PKI94_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNW26511.1"
                     /translation="MHIEELLEKVVEKKGSDLHISANLPPIMRVDGNLQRMDYPALTP
                     EDVEFLLFPMLSNEQRRRLEQEWELDFSYGIQGLSRFRVNFYKDKGNYAAAFRVIESI
                     VPSFESLGLPEVVRKTAEKPRGLVLVTGPTGSGKSTTLASMIDYINESKCEHILTIED
                     PIEFVHASKKSIVHQRELGQDTRSFANALKSALREDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAGQTIDRIIDVFPEGQQQQIRIQLSNSLVAVFAQTLLPRLQPDG
                     SQKGRVMAQEIMLVIPAIANLIREAKAAQIYSTIQMNNAIGMQTLEMSLKDLYQKKLI
                     TLDDAIARSSRPDELKRLLQA"
     gene            complement(7758..9404)
                     /locus_tag="PKI94_06970"
     CDS             complement(7758..9404)
                     /locus_tag="PKI94_06970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNW26512.1"
                     /translation="MKPFNKKVLWIFLAVLVLGTFLRLLGINKPEGLWNDEYVSWYVA
                     SIPFGKEFINQVFAQCHMPFYYLYLKFFMNFGQSDLALRLSSVFAGMLSIVVMYFVGR
                     EFKNKRLGIFCAIFTAISSFLIYYSQEVRMYEVLFLISAFSLLYTLKLVKNPKPHKIF
                     LYFLSLFLILFTHTIGFVYVFFSLFFVSYMLINKNVKYKKTISSLWIFIILGFLVFSP
                     LTFKIFTESSYSQWWGHFTFSKILFLISDYFSPVLTSLVNAPDNFFYNFSTKFFIFAI
                     LPSLLAIYGTYRAIRTGKTEALGLFLVVLGTLSVLSIAAAFGKMVFITKYSIEIYPIL
                     ITIFCFGISEVNNRKWKYFLALSFCFIQLLYLFTMPDSAIKRVRTEGHKLAADLILGA
                     ELKKGDFVLLEYYPKERFERYFDFEGLNVISINKGNFSQYLTDKELVKDKIEIKKQAR
                     EIFSANESNYFDKKLKKEIFQKMKKGQKISVIFLNSVSMISPNNLKKIAFDDKAYEKT
                     PFLFSVFSYLKNETIESMLTELKPVKLEQKGSWLCATFKK"
     gene            9486..10427
                     /locus_tag="PKI94_06975"
     CDS             9486..10427
                     /locus_tag="PKI94_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW26513.1"
                     /translation="MKLIIQIPCYNEEETISKTLSDLPEKIEGIDEIQVLIINDGSTD
                     KTIEAAKASGVENFIDFQTNRGLARAFSAGLAKSLDLGADIIVNTDADNQYCADDIQK
                     LIKPILEKKADIVIGTRPVEEIKHFSFTKKMLQKLGSWVMRVISSTDIQDAPSGFRAF
                     SKDAALKINIFDNYTYTLETIIQARAKGLKIACVPVRVNPELRKSKLMKNMFDYIRRS
                     SFTMMRMFIVYRPFRFFAIIGSIFLLAGFFIGARFLYFYSHAQGSGHIQSLILSAILI
                     LTGVQTGLIAVFAELQSINRKIVEDIQTRVKKIELNK"
     gene            complement(10435..11913)
                     /locus_tag="PKI94_06980"
     CDS             complement(10435..11913)
                     /locus_tag="PKI94_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26514.1"
                     /translation="MNVQIKNKFYFGVLILIFLLGFFLRAKGLLSNPSMWHDESALAW
                     NVKFKSYFDLLCAHLRFLQIAPPLFMVFAKLMTWLFGVTNTVGEFDFVVRLIPFLFSI
                     LSVFVFYFILKDVFNMKKTIIFAMFLFAINNVLVNYSFEFKPYCSDMFFVMLVLLIFL
                     KIDFLKINLKKILLISMILSSFVWFSFVSLFAIGAGLIYLIFKKTDFKRISVLFLPFL
                     LNFLLYSKIYLLGAYINNKNGMVTFWADKFISADFSNFIYLFSQNITYFFIPLKSALF
                     IVILALYGIFIFVKEKRFNFVIMALLIFSLSVASSILHIYPFSQRLIIFFIPLIIIFI
                     SKPLDQISLSKKVKSIMILMLLFFITFPQIKKTSESFTSVKINKGHFGKEMMQYLAQN
                     IKPDDIILFNSGSNADFYYYSSFYNLKNKFIQEKVNEDKDEAYLKFLQGLKKGNYWFF
                     MPYDYTSERRVTNCFMNWAYKNQKVLSKAESTRSVLMYVKIR"
     gene            complement(12003..>12856)
                     /locus_tag="PKI94_06985"
     CDS             complement(12003..>12856)
                     /locus_tag="PKI94_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNW26515.1"
                     /translation="FENAKQYLEENDGAGNTFKATSKPVLIGTAVVGATTMIFSLILL
                     IKETLGIQPEMILNVLNPYTLLGFISGGAVIYWFSGASMQAVTTGAYSAVEFIKKHIK
                     LGENDDKKANVENSKQVVKICTQYAQKGMVNIFISLFSFALAFAFLSAPFNGNNAPVA
                     FFVSYLIAIAVFGLFQAVFMANAGGCWDNAKKIVEVDLKEKGTPLHDATVIGDTVGDP
                     FKDTSSVAMNPIIKFTTLFGLLAMEIAISPAFNKYAHIAGIVFFVIALIFVIRSFYAM
                     RIPAKTE"
BASE COUNT         4581 a         2291 c         1953 g         4031 t
ORIGIN      
        1 gatggagcaa caaaacaatc tgtaattgtt atttctttcg tgtcaattac ttcatattct
       61 attccattta tgttctttat tgacatattt tcatcttcca aatttggtaa tatccatttg
      121 ttttaaaatt gcaatcaaaa catcagaaac tatactattc ccagcctgac gatacatttg
      181 tgtatcgctt attacaattt taaaatcatc tctaaaaccc attaatctta aacattctcg
      241 tggagttaat ttccttattc gacctttatt atgtgtaaca taattgtcaa ctcctgctct
      301 atgcattttg tgcattgttt ggagcaatgg tcttgctatt tctaagtcag ttttatcact
      361 tgttttaaag tttttcgttc cgccagctaa aacataattt ctaattttat ctgataaata
      421 atatttttct tctactttat ttacatcaaa aataaattca tcaaaatcat tgtgataaac
      481 attttcaaat acaaagtcac catgccaatt aaattgttgg ttagcttttt ggcataaggc
      541 tattgttcca tttatttgag tgtatctttt tgctctattt tttgaacttg taacaaattt
      601 cacacccttt tcttttaaat aatattttgt ttctgtataa tcttcaagaa aatcttgcat
      661 agtatattcc aaaggaatgg gcttgggaaa ttcaaaatta attgaagtat ctttaaatcc
      721 tacaacaatt atacgttcac gatgctgggg aatcccataa tctttactat ttaaaatttg
      781 aaaatttatt ttatacccta atgcttgaaa aacttcttga accacttgcc aagttttacc
      841 gccatcatga ttaattaaac ccttaacatt ttcatagata aatacttttg gttcgacctc
      901 ttggactatt ctagcaaaat cataaaataa agtccctctg acatcctcta aaccttttct
      961 tttaccaacc atagaaaaag cttgacatgg gctgccgcca acaagtaaat caactaatcc
     1021 tttatacttt gttgcagaaa attttgttat gtcatcatgc caatcttctt cattaatatt
     1081 ataatttgca aaataacttg tttttacatg aggatcaata tcaccggcaa atattattga
     1141 atgttttata tttaatcttt tcatcgcctg ttcaatagag ccaataccac taaataatgt
     1201 tgctaatttt atttcttttt caggataaga aaatatttta tccctccaat catgaatgtg
     1261 aaaagggaat attttggcac tatttttttg tcttttttct ttaagtacaa catttttcga
     1321 catatataaa tcctctatac caatgttacc acataattac aaacataaat aaatatttgc
     1381 ccatatcatt gaaataacaa caaattcaat aattgttaat tttcatttca tctttaaaaa
     1441 ttaaatgctc ttatatttta gtttatcctt ttcaagtctt tctttaagag cacccttagg
     1501 ctcgtagtaa ggtgaaaccg tcattatttt tgccgctaca tcgggataat gataaatgaa
     1561 tttcaattca ctgtcggtca aaggtttttg agtgtggacg tttgcataac gagccaattc
     1621 aagaacattt cttgcctcgt gctcatcttt aaattcaatt tcaagccctt cataaacgtt
     1681 tctgaaccct ttgatgccac cgtattctcc gacggtaatc attctcccgg tttcgattat
     1741 ttcaggctca cccctgccta attcctcgta atcgtaaagt tcataatttc ttctgtcttt
     1801 aagagccccg tctgcgtgaa ttcctgattc atgagcaaaa gcattgtctc ccactgcggg
     1861 ctggttcata ggaataggaa caccaaaagc atatgaagta tattttgcaa gcttccaagc
     1921 tttgctcaaa tcgattttag ggtcaatttc atattttccg ccaaatcccg cagacttaag
     1981 cactgccaaa agacaagaga ttaaatccgc atttccggca cgttctccca taccgtttat
     2041 agcagtatta atgtaagcat tcataccggc gtcaaccgcc gctttcgcac ccattacaga
     2101 gcaagcaacc gccattccca aatcattatg ataatgtgtt tctacatcga tttgtgcttc
     2161 agatgcaatc ctgtaaatcc tatcataagc cgttttagga tcttcatagc ccaaagtatc
     2221 acaatatctg aatcggtgcg caccacagga ttttgcttct tttgcatact taattaaaaa
     2281 ttccaaatcg cttcttgaag catcttctgc gttgactccg attaatttta cacctttaga
     2341 aacagcacat tctacagctt cacaagtcat tttcaaaata tcatcaagag ttttctttcc
     2401 ttgatatttg ccttgtgtca tctgcattga agttgactga gataaattca aatttttcaa
     2461 tttcggaaca ttttttaaag acagctcaac atcttctgca atcgccctca tccaaccgga
     2521 taaagttatg tttttaaaag cacctctttc aacaagttcc aaatttccgt tcagataatt
     2581 aatttcgtgc ttggtggtcg gaaaaccgaa ttctgattga gttattccca tatcatcgag
     2641 cataagatta attatggttt tttcaagctt tgccaagccc aatcttgaag tttgcacccc
     2701 gtctctgttt gttacatcta taaaataaat ttttcccatt atccaacccc tttattgcct
     2761 tattacaata ttatacacaa aaatttgaat tatataaaaa atgtgtttta atgttgtagt
     2821 caaatttttg ggaggtaaag agatgaaaaa aataggactt ttaggaggat taagccccga
     2881 atccacagtt gagtattaca aaattataaa ctctgaagtc agaaaggttt taggaggatg
     2941 ttcttcggct gagcttataa ttgaaagttt taatttcagg gaaatcgaaa aaatgcaatt
     3001 tctgggtaaa tgggacgaat tgggcgaaaa acttacccat gcggctaaaa accttgaaaa
     3061 atgcggagct gactacattg ttatttgcac aaatcttatg cacaaagttg caccttatgt
     3121 taaaaataga atttctgtgc cgttaatcca catggttgac agtgttgccc aagacattca
     3181 aagacaaaat atgaataaag tcggactttt gggaacaatt tttacaatgg aagaagattt
     3241 ttattccaaa aaacttcttg aagattattc gatagaaaca attattccag atagcaaaga
     3301 cagacaagaa ataagcagaa taatttatga agagctttgc tgcggaatta ttgatgaaaa
     3361 ttcacggaaa ttttatctta atgtaataga caaaatgaaa caaaacggcg cacaaggtgt
     3421 aatcttaggg tgtaccgaaa tccctctgat gatacaaaaa gcaagtattc cgatatttaa
     3481 tacaacgcgc atccatgcaa aaagcgttgt tgaaaaaatt ctggaaaaag aacctttact
     3541 attggcttaa atttaaaaca actgcggcgg ggaaattttc aattttcccg ccgcctcgta
     3601 atataagacg gctagtgtcg aatggctaaa cgacactcca cgtgccgatt tagccattct
     3661 cacctttgta agatcgacca ctgcacaagc gagaaaaaac aaattttcta cacttgtgca
     3721 gcggtcttaa catcttaaca acttgcaact tttaccataa ttatctaaaa tagataacat
     3781 gggtgaagta agaagaaatc agaaaattca aatttcgttc aaaacaaatg acggaacgga
     3841 aaaaattttt gaatgcaaaa ttaaggaatt atattccgac aggctgatgc ttgattatcc
     3901 caaagatatt gttttttacg ctgaatatct gtcagaagga gaagaaatat atgttaaaat
     3961 tttcacaccc gcagggattc gtgcatacaa tgcgcttatt ttagattctc ctttatccga
     4021 aaattttatc atagaatacg ttgacagtaa tattcaaatt caaagaaggg cttatgcaag
     4081 agcccctctt aaaacaaaaa ttattataga aaggtttgac gaacctaaca ttgtaacaca
     4141 tactgtcgat atcggcggcg gcggaataag attttatttt gacggacatt ttaacaggaa
     4201 tgaattagta aattgtaggc tatatttgcc tttgcagatg acatcagtaa aagcgcaagg
     4261 acatataatt gaaaagcccc atttacaaac gggcgaacat gtgattttat ttaataagat
     4321 aaacgaattt gacagagata aaataattaa aaaatgcttt gaaatagaag cgatgaaata
     4381 cagagaatac cagtaaaaag cgaagactat gaaacaaaaa atacaaaaag aaaaaaatat
     4441 cagaaaaaca gattttaaaa cagacaatgt tcaaaaggcg atagaacaat gccaaattgc
     4501 attacaaaaa gatcctacta acgcggattt acacgtaagg ctcggcgatt tatatttggc
     4561 gtggcacttg gatatatata actcatacca atacatagat gaagctataa ccgaatatca
     4621 aagagcttta gaaacatata ttgattcggc agaaatttat tttaaaatag gacaggcctt
     4681 tttctataaa ggggatttgg acaaagcaat caattacctg aatatgtctt taaaaaaaga
     4741 tgcaaaaatg gcaaaaactt actactttct tgcagaaact tacacaaaaa aagcacgttt
     4801 tgccgatgca atttataatg caaaaaaatc aataaagtta aaaccgttta gcaattcctg
     4861 ctctcacttc ttacttcata acctttataa actttcttct ttcaaaagtt ttaaaaaaag
     4921 attaatgtca aaattcgaat ttttgctttc attgttgacg cttccttttg ataaagcggc
     4981 aattaaaaat gttatacaaa atttaagtta tctgcgattt atccctattt taatgaaagg
     5041 ttattaccaa attcaaacaa aaggatttac tcaagctgtt gatatttata tcgaagccat
     5101 tgaaaaagca ccgggctttg tgcctttata ttgtctttta ggtgatattt acagagccat
     5161 aggcgacttt gaagacgcca taaccgaata caaaatggca atttggctcg acagtttaaa
     5221 tattcccgca tatattcatc tttgtcaggc ttatgaagag cagggggact atgacaatgc
     5281 aatagagact tataaaaaat tgattgaaat catgcctcac cgccccgaat ttcactcaaa
     5341 ccttgcaaat atttatttct taaaaggtga agtcgatttg gcagtttcac attatcaggc
     5401 ggctataatt ttaaatcccg gcaagcaatg gacaagtgtc atagcacaaa ctttaggttt
     5461 tgtgcatcag gaaacaaaag aggacatgga tgctgcaata agtgcttatc aaagtgctta
     5521 tcttctgaca cccgatgata ttgacattta cgttaattta ggaagtgctt tttatgacaa
     5581 agaagattac gacaatgcac ttacaactta cagagcagct ttggaattgg acccggtaaa
     5641 tgccaaaatc cactgtaatt tgggatttct acactggggc aaaggcgaca ccgatgaagc
     5701 cataaaagaa tacgaacttg caataaaata cgaccccacc tatgatgttg catacaataa
     5761 tttgggagta atttaccttg acgatttagg aagagttcaa caagcaatag aattatttac
     5821 aaaatctatt gaatataatc ccaactacgc acttgcacat tttaatcttg caagggcaat
     5881 cacaatcaaa ggtgataaga ttgaagcagc aaagctttat caaatagccc aagacataaa
     5941 caaaattacc aacgaaatag atccgcaaga aataataaat aaaattaacg gattatttca
     6001 gtcttaaatt attgatagaa ttgagccaaa ttagccaagc gcaattcttt aagcgaaatt
     6061 tcttcaagtt gctcttcaat caatttaagc tctgcgctta acgcatctat atctttatgc
     6121 tcggctttaa caatcggtgc agcttttgcc ggtttaactt caaattccac ccaacctgca
     6181 aaaaacggag cattttcaaa tcttttttca aaatcatcat acatatttgg cataactttc
     6241 tcctactttg caaatacagc ctgaagcata tctacgacat caaaattgcc gaatttaaaa
     6301 gtatattttt gatgtgcata ctcttgagga atttcttttt gtgcacagca ttcaaaataa
     6361 tccggcgaat taaaattaaa acttgtcatc tctgaatagt caatcatcgc ttcatcaatg
     6421 ttaaaaaatt tatccatttt ttataccctt tccacagatt aaagtgattt tataccactt
     6481 aatcgttgag gcttatccct cacacatatc ctaacaattt atttgtcggc tatttaaaaa
     6541 ttttctttat atttaggaat aaaattttaa ccttttgtta caatcaaaaa aatttatgca
     6601 aaatatacta taatgtaaaa atagatagga gattggttaa atgcatattg aagaattact
     6661 ggaaaaagtt gtagaaaaaa aaggctccga cctgcatata tccgcaaatt tgcctcccat
     6721 aatgcgtgtt gacgggaatt tgcaaagaat ggactatccg gcactaacac ctgaagatgt
     6781 tgaatttctt cttttcccta tgctttctaa tgaacaaaga agaagacttg aacaagaatg
     6841 ggaactggat ttttcttacg gcatacaagg tttaagccgc ttcagggtaa atttctataa
     6901 agataaaggc aattacgccg cagctttcag ggtaattgaa tccattgttc cttcttttga
     6961 atcactcgga ttacctgaag tggttagaaa aaccgctgaa aaaccccgcg gacttgtttt
     7021 ggttacaggg cctacaggaa gcggtaaatc aacaacgctt gcgtccatga ttgactatat
     7081 taatgaatca aaatgcgaac atattttgac aatcgaagat cctattgaat ttgttcatgc
     7141 ttctaaaaaa agtatagtgc atcaaagaga attaggacaa gacacccgct cctttgcaaa
     7201 cgcgcttaaa tcagccttaa gagaagaccc tgacatcata ctggtgggtg agatgagaga
     7261 ccttgaaaca atacgccttg cacttactgc tgcagaaaca gggcacttgg tttttggaac
     7321 ccttcacaca tcttcagccg gccaaacgat tgaccgtatt attgatgtat tccccgaagg
     7381 acaacaacag caaatcagaa ttcagctttc caattcgctt gttgcggttt ttgcacaaac
     7441 tctcctgcca aggttacagc ctgacggaag ccaaaaaggt cgtgtcatgg ctcaagaaat
     7501 aatgcttgtt atacccgcaa tagcaaacct tataagagaa gctaaagcag cacaaatcta
     7561 ttctacaatt caaatgaata acgcaatagg catgcaaact cttgaaatgt cattgaaaga
     7621 tttatatcag aaaaaattga taacgcttga tgatgcaatc gcaaggtcct caagaccgga
     7681 tgaattaaaa cgtttactgc aagcgtaatt taatccaaca caaaggggcg gctttttaaa
     7741 aaagccgccc ctttgtgtta ttttttaaaa gtagcacaaa gccaggagcc tttttgttct
     7801 aattttactg gctttaactc agttaacatt gattctattg tttcattttt caagtaagaa
     7861 aaaactgaaa acaaaaacgg tgttttttca tatgctttat catcaaaagc aattttcttt
     7921 aaattatttg gggaaatcat agaaacgcta tttaaaaaaa taacgctgat tttttgcccc
     7981 tttttcattt tttgaaaaat ctcttttttt aatttcttat caaaataatt actttcattt
     8041 gctgagaaaa tttctctagc ttgttttttt atttcaattt tatccttgac tagttcttta
     8101 tctgtcaaat attgcgaaaa atttccctta tttattgaaa taacatttaa tccttcaaaa
     8161 tcaaaatatc tttcaaacct ttctttagga tagtattcca gcaaaacaaa atcacccttt
     8221 tttaattcag caccgagtat caaatctgca gcaagcttat gtccttcggt gcgaactctt
     8281 tttattgcag aatcaggcat tgtaaaaaga taaagaagtt gaataaaaca aaaagataaa
     8341 gcaagaaaat acttccactt tctgttattc acttctgaaa ttccgaaaca aaaaattgtt
     8401 atcaaaatcg gataaatttc tattgaatat tttgtaataa aaaccatttt tccgaaagca
     8461 gcggctatgg aaagaacact caaagttcca agcacaacca agaaaagacc taatgcctct
     8521 gttttgccgg ttcgtattgc cctgtaagta ccgtaaattg ccaataaaga aggtaaaata
     8581 gcaaaaataa agaatttggt cgaaaaatta taaaagaaat tatccggtgc attaaccaaa
     8641 cttgttaaaa caggcgaaaa ataatcgcta atcaaaaaca aaatttttga aaaagtaaaa
     8701 tgtccccacc actgtgaata agatgattct gtaaatattt taaacgtaag cggtgagaag
     8761 acaagaaaac ccaatataat aaaaatccaa agcgaagaaa ttgttttttt gtatttaaca
     8821 tttttattta taagcatgta actcacaaaa aataaactga aaaacacata aacaaaacct
     8881 attgtatgcg taaaaagaat taaaaacagc gaaagaaaat ataaaaatat tttgtgaggt
     8941 ttaggatttt ttacaagctt aagtgtatat aaaagagaaa aagcactgat taaaaaaagt
     9001 acttcataca ttctgacttc ctgagaatag tagattaaaa atgagcttat cgcagtaaaa
     9061 atcgcacaaa atatacccaa acgtttattt ttaaattccc ttcccacaaa gtacatcaca
     9121 acaattgata acattcctgc aaaaaccgaa cttaagcgca gagccaagtc gctttgccca
     9181 aaattcatga aaaatttcaa atataagtaa taaaaaggca tgtggcattg tgcaaagacc
     9241 tgattaataa attcttttcc aaaaggaatt gatgcaacat accagcttac gtattcatca
     9301 ttccaaagcc cttcaggctt gttaattcca agcaatcgca aaaatgtacc caaaactaaa
     9361 actgccaaaa atatccatag aacttttttg ttaaaaggtt tcatacaaca ctcctatttt
     9421 taaaaaattt gcaactctcc aaatttttaa tataattata taataaagat tggaaattta
     9481 tacaaatgaa gttaataatt caaatacctt gttacaatga agaagaaact atctcaaaaa
     9541 cacttagcga tttgcctgaa aaaattgaag gcatagatga aatccaagtt ttaattataa
     9601 atgacggaag cacagataaa acaatagaag ctgcaaaagc aagcggagtg gaaaatttta
     9661 ttgatttcca aacaaatcgg gggcttgcaa gggcttttag tgcgggattg gctaaatctt
     9721 tggatttagg agctgacata attgtaaata cagatgcaga taaccaatat tgcgcagatg
     9781 atattcaaaa gttgattaag cctattcttg aaaaaaaagc cgatatagtt atcggaacac
     9841 gtcctgttga agaaataaag cacttttcct tcacaaaaaa aatgcttcaa aaactgggtt
     9901 catgggttat gagagttata agttcaaccg atattcaaga cgcccccagc ggctttagag
     9961 cattctcaaa agatgctgcg cttaaaatta atatatttga caattacact tatacccttg
    10021 aaacaataat tcaggcaaga gcaaaggggc ttaaaattgc atgtgtccct gttcgagtta
    10081 atcccgaact tcgcaaatca aaattaatga aaaatatgtt tgactatatc agacgctcaa
    10141 gttttacaat gatgaggatg tttatcgtgt acagaccatt cagatttttt gccattatcg
    10201 gctcaatctt tttacttgcc ggctttttta tcggcgcaag atttttgtat ttttactccc
    10261 acgcgcaagg ctcagggcat attcaatccc ttatcctttc tgcaatactc attttaaccg
    10321 gtgtccaaac aggtttaatt gcggtttttg ccgagcttca atcgattaac agaaaaatag
    10381 tcgaagacat ccaaacaaga gttaaaaaaa tagaattaaa taaatagtcg agttttatcg
    10441 gatttttacg tacattaaaa cacttcttgt tgattcggct ttgcttaata ctttttggtt
    10501 tttgtatgcc cagttcataa agcaatttgt aactcttctt tcggaagtat aatcgtatgg
    10561 cataaaaaac caataattgc ctttttttaa cccttgtaaa aattttaaat acgcttcatc
    10621 tttatcttca ttaacttttt cttgtataaa tttgtttttc aaattataaa aagatgaata
    10681 gtaataaaaa tcggcattag acccgctgtt aaaaagaata atgtcatctg gttttatatt
    10741 ttgcgccaaa tattgcatca tttctttgcc aaaatgccct ttattaattt ttacggaagt
    10801 aaaactttca gatgtttttt taatttgagg aaacgtaata aaaaacaaca acatcaaaat
    10861 cattattgat ttcacttttt tgcttaaact tatttggtca agcggttttg aaataaatat
    10921 tataataagc gggataaaaa atattattaa cctttgagaa aaggggtaaa tatgaagaat
    10981 agaagaggct acggataatg aaaaaatcaa taaagccata attacaaaat taaagcgttt
    11041 ttcttttacg aagataaaaa tcccatacaa ggctaaaatt acaataaata aagctgattt
    11101 taacggaata aaaaagtaag ttatgttttg tgaaaacaaa taaataaaat tagaaaaatc
    11161 agccgatata aatttatctg cccaaaaggt caccattcca ttcttgttat taatataagc
    11221 accaagcaga tagatttttg aatacagcaa aaaattcaac aaaaaaggca aaaataatac
    11281 tgaaattctc ttaaaatctg tttttttaaa tattaaatat attaagcctg cccctatcgc
    11341 aaataaagac acaaaagaaa accaaacaaa acttgacaaa atcattgaaa ttaaaagaat
    11401 tttttttaaa ttaattttta aaaaatcaat ttttaaaaat atcaacaaga ctaacataac
    11461 aaaaaacatg tctgagcaat aaggcttaaa ttcaaaggaa taatttacta aaacattgtt
    11521 tattgcaaac aaaaacatag caaaaatgat tgtttttttc atattaaaaa catctttaag
    11581 aataaagtaa aaaacaaaca cggataggat tgaaaataaa aaaggaatta atctgacaac
    11641 aaagtcaaat tccccgaccg tatttgtaac gccaaaaagc catgtcatta acttagcaaa
    11701 aaccataaat aaaggcgggg ctatctgcaa aaatctcaga tgtgcgcata ataaatcaaa
    11761 ataactttta aacttgacat tccaagccaa agcagactcg tcatgccaca ttgaaggatt
    11821 tgacaaaagc cccttggctc ttaaaaaaaa ccctaacaaa aatattaaaa ttaaaacccc
    11881 aaaataaaat ttatttttaa tctgaacatt catataccaa ttttaacaaa aaaaattaaa
    11941 aaaggcggct tcggtttttt aaaaccgaag ccgcctaata ttaaacagaa aaatgtaaat
    12001 atctattctg ttttagcagg gattctcatt gcgtagaaag aacgaattac gaatatcaac
    12061 gcaatcacaa agaacacgat acctgcgata tgcgcgtatt tgttaaacgc aggtgaaatc
    12121 gcaatttcca ttgccaacaa tccgaacaat gttgtgaatt taataatcgg attcatcgca
    12181 acagatgaag tatctttgaa aggatcgcct acggtgtcac cgattacggt ggcatcatga
    12241 agaggagttc ctttttcttt caaatcgact tcaacgattt tcttagcgtt atcccagcat
    12301 ccgccggcat ttgccataaa tacagcctgg aacaagccaa atactgctat tgcaatcaag
    12361 tagctgacaa agaacgctac aggagcgttg ttcccgttaa atggagctga caagaaagca
    12421 aaagcaagtg caaatgagaa cagagaaata aatatgttca ccataccttt ttgagcgtat
    12481 tgtgtacaaa tttttactac ttgttttgaa ttttcaacat tggctttttt atcgtcattt
    12541 tcgccgagtt ttatgtgttt tttaataaac tcaaccgctg aatatgcacc tgttgtaaca
    12601 gcttgcatag aagcacctga gaaccaataa attacagcac cgccggaaat aaatcctaat
    12661 aaagtatatg gatttaatac gttaagtatc atttcaggct gaatgcctaa agtttcttta
    12721 attaataata tcaaagaaaa aatcatggta gtagcaccga ctaccgcagt accgataaga
    12781 accggttttg aagtagcctt gaatgtgttt cctgcaccgt cgttttcttc taagtattgt
    12841 tttgcatttt caaagt
//
LOCUS       DAOQAU010000048        12854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5390_length_12854_cov_8.211657, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000048 DAOQAU010000000
VERSION     DAOQAU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5390_length_12854_cov_8.211657"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..479)
                     /locus_tag="PKI94_06990"
     CDS             complement(84..479)
                     /locus_tag="PKI94_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW26516.1"
                     /translation="MKSLVVDDDFFSRRILQTIFSGYGECHVAVDGKEALFAFEQATA
                     EESPYDVICLDIMMPEMDGQEVLKKIREFEERKGIFGSDSVKIIMTTALDDSENIKKA
                     FREQCESYLIKPISKSKLISILKDFKLID"
     gene            669..992
                     /gene="trxA"
                     /locus_tag="PKI94_06995"
     CDS             669..992
                     /gene="trxA"
                     /locus_tag="PKI94_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNW26517.1"
                     /translation="MTNVIELNDVNFDAEVKRSTQPVLVDFYATWCGPCRMQLPIMDE
                     VAKDFSGKALIGKLNVDNGKNKSAQYGIASIPALLVFKDGDVVEKLTGLHSRSQLAEI
                     LKKHL"
     gene            1059..1616
                     /locus_tag="PKI94_07000"
     CDS             1059..1616
                     /locus_tag="PKI94_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNW26518.1"
                     /translation="MNSVIQNILNRRSVRVYSDEPVKKSDLDLILKAGMYAPSGCNMQ
                     PWHFTVIQDKELIKTLSNESKKEFINHENEMFRKMAANKDYNIFYNAPMIIVISGEKS
                     SPTSIIDCSAATQNMILASESMSMGTCWIGLITFLFKGKNGKKWAEKINIPENYEPYY
                     AITLGYKKYPDPKAPDRRKNVINYI"
     gene            1832..7507
                     /locus_tag="PKI94_07005"
     CDS             1832..7507
                     /locus_tag="PKI94_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26519.1"
                     /translation="MLIKTGAENQKVNNIKNTKLNFISFSQRQRNERNILLELTSNSG
                     SISDIRSRQIKTILEDIAKNGDDEDISLLLSVTKNLKYGYFENSPLGNFLNTKSNIAQ
                     MDQKQNNNWANLLENTLRTSLEKSTSPKKADFIKQLNQIFSKTENENFLITNKEISSN
                     PVFQQQAQAIKYRNKILNSYAFNTPPKGLSPKESEDFEIDRQRTKRNIDYFLASSECT
                     LSEKIEVLNKLSIMMSREYSINKQLKDKKVKVLSEILNDIVIRTPENIRPNIKDVDQV
                     RHGICAAISYARKAVAYEDKVAYINSIYMELDDNPQMYVFDITKLGKKIKIPIAKTYI
                     DYNSLIKKGYRIIDSSVFQWMNVAGTTGSGSNSVEYYIGYDSQNYEMFNDSKMYPDLE
                     GEDGTIQNYLRTLSKTRKDLQSITESKILDNIGAKEAYKNFNKNIEVISSIYQKAFDI
                     LKQEFPSKSSEELRKISKSVMKIQEAEDENFRIHPKEAEVLKKQKIITLVKSFVGNID
                     KEKGNRLGEELYQLYDLYSQYSAQLPSSKGLNKKAKEYKRLFKIAADIRICTEKSCLV
                     KENNDSFCAEFNLPSDVQLISKRLEDLIKDLEENNNTDKVAKILGKEPNKDSLLKYLK
                     KMQKEFEVNIPNKIDMILKALGIGTRQHFLNSLLTADLANFLNNPTNDLIDYYASILK
                     VKPEKEIIEKTLKQVSQELQNPISEKRIVEIGNIFGIGNYLSIADGLIDQFMAQELSE
                     STVKNLSIAFDIENSQVEVFEKLSEVKGLIEQLYYSQIEISQKIKAPNSVQMILKKLE
                     KRGDILSRETLDKLKAKFDSIEQYEINAEKALKRGENTPPDKNIYKFNQEEEAILKRI
                     ESMLPSMTKVVEKEYKKYNKIISNDLNTLYDYIGRQKGRFWLSEEGHSGLFSGEQVRI
                     AEQMTDRPYYINENLLSSIKQIKETGKSGVMGTNVDYDTFSGHAQYIGDIKTMLVADS
                     KTGEISQKEVLFQDNSWGAGEKVRFQSKNTPFWKDLAGYERTNYASADLCGGPKGFIL
                     DSTTWQTGLEVNDLLSDYGINKPYVPENKKLKKLIDFQNETFPIFNDIVLEGDSPKLY
                     TEFLKLIDSVFEIGTDEKKLNELINILQKNKNVKINTDKLEKIEDSAEQIEQKWFSFI
                     KGSNQLPQAAYSKEIISKMSLEEKIKLFGIDSKETFDNLPQNHKLKLLLRKIGLYDLL
                     LGETFEDKISTAKTHQELDEVEDKILENIKKLIKESLKEIKTPREEIKKIKDITENFE
                     YSYVLINWIDKKFNPKTDEELIDKLFELQTKPNLMKQALEESTRVELGMKIADPYDLI
                     KQIRAENYRAESKLNKAIFSDLIGTQYDLYNNTKSPQQKSEQLYRSLFIAMSYLDKKT
                     IKKYKEMFFNKYQVRPAFPEIPVIDKEEVNKVNKEWEDFFIETVQTISDMKKFAKLEE
                     LYQQAQAGADIIRNNYNKESVIALYPILNEMKNVFADEDEEIKNILDNIIKSNPKDFI
                     ANFAELKKTMEEIQKQLPQKDMKKLIGNSIKNTNTSCEIMIKSYIQPKHQSQMKEVIN
                     NLIRSLYKNENRNAPENQHALNLFRQNLEKCSVLNNPIDILHNILNILENPSQNKSVN
                     LSVMTNLKNYANRAFRASDLTEIEYKIMKNIQNGNITRFKRLMGRDYLVGQDKTKFKM
                     DSKDGLGTILLNLLDEKNQNRTLKLFVESLGLNEEIVSFFNQVIKPEEQLKSLLDTIQ
                     EAKNAINDRNLIKEVLTKGLNAKDMSNIPSQVFEQIKEKLTKNNVGEEKTISKFLQIY
                     CRVMEENLAANKQPFSRNEVINVVNKAHIKAFNKAYANFQENLEDINTNFTDLQSIEE
                     IITTLSLNEDSETYKQAQNYQQELIKVIQKIAKEIEKLNSMTPAEEEEE"
     gene            7621..9288
                     /locus_tag="PKI94_07010"
     CDS             7621..9288
                     /locus_tag="PKI94_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26520.1"
                     /translation="MPSINSNYDKKISFTGLTSYLRKTVFPCDDVKKLATDIFDNSFA
                     GNLPSEWIEKIPKNQRTQTIKDVFQGFREFVQLINEKSETALSQLEKTLKGAGILSYD
                     KSINLKFEGSGSYGRVYRLEGLENQKTGEQYVIKLYNKAQKNAQNNGIFAELNRGGLY
                     WPEALGKKTQMVKTFFADLESGFVVNKFIDDSLASPKNIVPSKLLGLESYDDHPKQNV
                     LNNLYSLNVINGYVIDQGCLRAVCPEIINSKDARYVIKKIFFTPKNKAYEKWLELLNN
                     PAYKHNSSVKVALIEAMEYLQPGDRTKCFNEILERFNKDQKLKHYLTKNIKFIQDEDF
                     APCFAKLWSQSEPVEKNELIKNIDRLPQKMQKNYISEAIKEKTLIPEVVEKFDNLPKE
                     ELADLFNSAIKESDDESKIKLFEKLFLLPEELRLEYAKKILQNIENMNILGKVEEKTY
                     LLRLNGSQDYKKAYEFLITDAKDTTKVFIISKVLKNLDFEDYVKAVKLLAKDSSEAVK
                     FKLAPTLEKIIENKSAKREDLDFIYQELAKNTNNQNYLDYIKEVFQH"
     gene            9394..10644
                     /locus_tag="PKI94_07015"
     CDS             9394..10644
                     /locus_tag="PKI94_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNW26521.1"
                     /translation="MPEPFFNEKKDTFNNIINVIQETLDVNKTKNIIIETICKTFNAN
                     RCCIVDYDKRLNKFLPIEHEYISSDKVSSCRGQDVQTIVPGLVEAAKKGKFILVKNKE
                     IFFDTDKQVLQTEQDTFEKYDINSLLVLPLFYQNELLGILSAHYTNGHEITDSEINII
                     KNIGNQAVLAIHRANLYEMNQAQLKKAEATKKIIEASRKSLELEVLLSDICKELAELF
                     KFQRATIGQLIRETPEESVAVGEYIYNKNIKSYKSHSGFGEIIKYWSSYLMREKKTKI
                     VNDISNSDMPAKVAQIYKEIGINSIACIPLRSEENMIWGGMFLSEKEDNRNVIEENLE
                     FLETVGSQIYIAIRQAELYKKEKEALERESLLRKITSAIRSTLDIDELEKIIVEKIAK
                     ALRADRCFIIAYDDETNKFLPVKT"
     gene            10672..11724
                     /locus_tag="PKI94_07020"
     CDS             10672..11724
                     /locus_tag="PKI94_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNW26522.1"
                     /translation="MVGFDIEKELPDLIAKNKNNEDVIITDLDKFLAQNNFYNTPIEK
                     HFREYKTQSGFATSIFYSQKLLGVLAIHYLNKKGKFIEEEIQLVKALADQIGIAINQA
                     RLYKSEKEAKEKMSSSIIEIMRKSLDKTMIKKLFVKNIRKFFKADRVFLSEYDAEKKR
                     YLPADKDSEYLSNYGEKSFVQYAWNNPEELEYIEPLLNKEELIIYNWDEYIKQNKKSP
                     GFIKLFEDAGVKSSYNFPILHYQNIMGLLCMEFTTEVVRLTEEELNIIRNICTQVGIA
                     LYQAKLYLEAQQCFFSKEQLLEKVSEKIEPPLKTILQEVSHLSENNTVREEQVNNIID
                     NCSLIMNSTKEVSQEI"
     gene            complement(11808..12665)
                     /locus_tag="PKI94_07025"
     CDS             complement(11808..12665)
                     /locus_tag="PKI94_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW26523.1"
                     /translation="MDCLNCEKGCNGGTGTDCKELSTGALESLIEARRQQMQRLYLDG
                     QNTSGMSEVDKDEIIQERIKDSIEEHWKPGLYDRNYVDRSKNKLVTQVPNSALEEIYH
                     AMHKYTPEDHKNCSACGYGNCKDMAIAIYNGLNNPHNCHYYQHKMLETNSEKRRNAMS
                     EFQSLILNEFNAEKLLAKFNPIVKAIEGIAFQTSILSINASIEAAHAGEIGAGFDIVA
                     KEVKSLAEKSKEEANKIYSSLSDLQSVLDGATEQFEAQLKAFLSEEDNQETDKNTKKE
                     SCEPASSLK"
     gene            complement(12744..12854)
                     /locus_tag="PKI94_07030"
                     /pseudo
     CDS             complement(12744..12854)
                     /locus_tag="PKI94_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         5105 a         1984 c         2015 g         3750 t
ORIGIN      
        1 cggcaaggca atatctatat attttaattt tttaattttt tccaagaaca atttttaatt
       61 ccttcgattt taagtattta aaattaatcg ataagtttaa aatcttttaa aatacttata
      121 agttttgatt tgctgatagg cttaatcaaa taagattcac actgctccct gaaagccttt
      181 tttatatttt cactatcgtc aagagctgtg gtcataatta tctttacact atcacttccg
      241 aatattcctt ttctttcttc aaattcgcga atttttttca aaacttcctg accgtccatc
      301 tcaggcatca ttatatcaag acaaattaca tcataaggtg actcttctgc cgttgcctgc
      361 tcaaaagcaa aaagagcttc ttttccgtcc acggcaacat gacattcacc gtagcctgaa
      421 aaaattgttt gaagtatccg cctgctaaaa aaatcatcat caactactaa gcttttcata
      481 aatttctcct tatatacaaa ataattaatt tttcaagacc aattctagca taattttttt
      541 tataaaaaat catacgttct tagtatgata aaagaatgtt ttaagcaaat aaaataataa
      601 agggttgaca ttttttaaat atatgttaat atgaatatat taatattaat atataaagga
      661 gatttattat gacaaatgtg attgaactaa acgatgtaaa ttttgatgcc gaagtaaaaa
      721 gaagcactca acctgttttg gttgattttt atgctacctg gtgcgggcct tgcagaatgc
      781 agcttccgat tatggatgaa gttgcaaaag atttttcggg aaaagcactc atcggaaaat
      841 taaatgtcga taacggaaaa aacaaatctg cccaatacgg tatcgcaagt attcctgcac
      901 ttcttgtttt taaagatgga gatgttgttg aaaagcttac cggtctgcat tcaagaagtc
      961 aacttgcgga aattttgaaa aaacaccttt aaatctgatt caaaattaat tgttttcatg
     1021 attaaataaa tcgtgtctga aaaacttgga ggcgatttat gaacagtgtt atacaaaaca
     1081 ttttaaacag aagaagtgtc agggtatatt ccgatgaacc tgtcaaaaaa agtgatttag
     1141 acctgattct caaggcaggt atgtatgcgc caagcggctg caacatgcag ccatggcatt
     1201 ttaccgttat tcaggataaa gagttgatta aaaccctcag taacgaatcc aaaaaagaat
     1261 ttataaacca cgaaaacgaa atgtttcgca aaatggctgc aaacaaagat tacaatattt
     1321 tttacaatgc ccctatgata attgtaatat caggtgaaaa aagttcacct acaagtataa
     1381 tagactgctc tgcggcaacg caaaacatga ttttagcttc agaatcaatg agtatgggca
     1441 cctgctggat tggcttgatt acttttcttt tcaagggaaa aaacgggaaa aaatgggctg
     1501 aaaaaattaa tataccggaa aattacgagc cttattacgc aatcacttta ggctataaaa
     1561 aataccccga ccctaaagcc cccgacagaa gaaaaaacgt tataaattac atttaatatt
     1621 gattgccttc agggcgggaa aatttatttt tcccgccctg aaagtgttta caaaatgtta
     1681 caaggctaaa agccttattt taagctataa aatgaattta tttctacttt tttttattaa
     1741 actagcaaaa aaaatcttta gatgttttaa aaaataacat ggcaaaatcg tgtgctgtaa
     1801 agtaaataca tttagaggat tttaaagggt tatgctgata aaaacaggtg ctgaaaatca
     1861 aaaagtaaac aatatcaaaa atacaaagtt aaactttatt tcttttagtc aaagacaaag
     1921 aaatgaaaga aatatccttt tagagcttac atcaaattcc ggctctattt cagacattag
     1981 aagccgacaa attaaaacaa ttttagaaga tattgcaaaa aatggagatg atgaagatat
     2041 ttctctttta ctttctgtta caaaaaatct gaaatacggc tattttgaga attcaccttt
     2101 aggcaatttt ttaaacacca aatcaaatat tgcacaaatg gatcaaaagc aaaataataa
     2161 ttgggcaaat cttctggaaa acaccctaag aacatcttta gaaaaaagca cttcacctaa
     2221 aaaagctgat tttataaaac agttaaacca aatattttcc aaaacggaaa atgaaaattt
     2281 tttaatcaca aataaagaaa tttcttccaa tcctgtattt cagcaacagg cacaagcaat
     2341 taaatataga aataaaatat taaattctta cgcctttaat actccgccaa aaggtttatc
     2401 cccgaaagaa tcagaggatt ttgaaataga ccgccaaaga acaaaaagga acattgatta
     2461 ctttttggca tcttcagaat gtactctgtc ggaaaaaatt gaagtattaa acaaattatc
     2521 aataatgatg agccgagaat attccataaa taaacaatta aaagataaaa aagttaaagt
     2581 tttatccgaa atactcaatg acatcgttat aagaactcct gaaaacataa gacctaatat
     2641 taaagatgtt gaccaagtaa gacacggtat atgtgccgca atttcatatg ccagaaaggc
     2701 tgtagcatac gaagacaaag ttgcttatat taattcaata tacatggaac ttgacgacaa
     2761 tcctcaaatg tatgtttttg acattacaaa attagggaaa aaaataaaaa tccctattgc
     2821 aaaaacttat atagactaca actctcttat aaaaaaaggc tatagaatta tagattcatc
     2881 tgtttttcaa tggatgaatg tagcaggaac aacaggaagc ggttcaaatt ctgttgaata
     2941 ttatataggc tacgacagcc aaaattacga aatgtttaac gattcaaaaa tgtatccgga
     3001 tttagaagga gaagatggaa ccattcaaaa ttatttgcga acattgtcca aaacaagaaa
     3061 agatcttcaa tctataacag aaagtaaaat acttgataat attggtgcaa aagaagctta
     3121 taaaaatttt aacaaaaaca ttgaagtaat ttcttctatt tatcaaaaag cctttgatat
     3181 tttaaaacaa gaatttcctt caaaatcttc tgaagaatta agaaaaattt ctaaaagtgt
     3241 tatgaaaatt caagaagcgg aagatgaaaa ttttagaata caccccaaag aagccgaagt
     3301 actaaaaaaa caaaaaatca tcacccttgt taaatctttt gttggcaaca ttgataaaga
     3361 aaaaggcaac agattaggtg aagaattata tcagctttat gatttgtatt ctcaatacag
     3421 tgcgcaactt ccttcaagta aaggtttgaa taaaaaagca aaagagtaca aaagactttt
     3481 caaaatagcc gcagatatta gaatctgtac tgaaaaaagt tgtcttgtaa aagaaaataa
     3541 tgactctttc tgtgcagaat ttaaccttcc gtctgatgtt caactcataa gcaaacgact
     3601 ggaagattta attaaagatc tcgaagaaaa caataatact gacaaagtcg caaaaatact
     3661 tggcaaagaa ccgaacaaag actccctatt aaaatatctt aaaaaaatgc aaaaggaatt
     3721 tgaggttaat ataccaaata aaattgatat gattttaaag gcacttggaa taggcaccag
     3781 acaacatttt ttaaattccc tattaactgc agacttggct aattttttaa ataatccgac
     3841 aaatgatttg atagattatt atgcttcaat tttaaaagta aaacccgaaa aagagattat
     3901 tgaaaaaaca ttaaaacaag tatctcagga gttgcaaaat cctatatcag aaaaaagaat
     3961 agttgaaata ggaaatattt ttggcattgg aaattattta tcaatagcag atgggcttat
     4021 agaccaattc atggcacagg aactttctga atcaacagtt aagaatttgt caatagcctt
     4081 tgatattgaa aactctcagg tagaagtttt tgaaaaatta tcagaagtaa aaggcttaat
     4141 agaacaactt tattatagtc aaatagaaat aagtcaaaaa attaaagccc ctaattctgt
     4201 tcaaatgata cttaaaaagc ttgaaaaaag aggagatatt ttatcaagag agactttaga
     4261 taaattaaaa gccaaatttg acagcatcga acagtatgaa ataaatgccg aaaaagcact
     4321 aaaaaggggt gaaaatactc cgccggacaa aaacatttat aaattcaatc aagaagaaga
     4381 agctatttta aaaagaattg aatcaatgct tccttcaatg acaaaagttg ttgaaaaaga
     4441 atataaaaaa tataataaaa tcatttcaaa tgatttaaac acattatacg attatattgg
     4501 aagacaaaaa ggcagatttt ggctttcaga agaagggcat tctggtcttt tttccggcga
     4561 gcaagtaaga atagcagaac aaatgacaga tagaccttac tatataaatg aaaatttatt
     4621 atcttcgatt aaacaaatta aagaaaccgg caaaagcgga gtaatgggta caaatgtcga
     4681 ttatgacact ttttcagggc atgcccaata cattggtgat ataaaaacaa tgctggttgc
     4741 cgattctaaa acaggagaaa tcagccaaaa agaagtttta ttccaagata acagctgggg
     4801 tgcaggagaa aaagtaagat tccaaagcaa aaacactccc ttctggaaag atttggcagg
     4861 ctatgaaaga actaactatg cttcagccga cctttgcggg ggacctaaag gatttattct
     4921 cgacagtacg acatggcaaa ccggactcga agtaaatgac ttgttatctg attacggaat
     4981 taacaaacct tatgtcccgg aaaataaaaa actgaaaaaa ttgatagatt tccaaaatga
     5041 aacttttcct atttttaatg acatagtact cgaaggggac agccctaagc tttatactga
     5101 atttcttaaa cttattgaca gcgtttttga gatcggcaca gatgaaaaaa agctcaatga
     5161 actgataaac atattacaaa aaaataaaaa tgttaaaatc aatacagata aacttgaaaa
     5221 aattgaagac agtgccgaac aaatcgaaca aaaatggttt tcttttataa aaggttcaaa
     5281 ccaactgcct caagcagctt attctaaaga aattatttct aaaatgtcct tggaagaaaa
     5341 aattaaactc tttggaattg attcaaaaga aacttttgat aatttgcccc aaaatcataa
     5401 attaaaactt cttttaagaa aaataggttt atacgattta cttttggggg aaacctttga
     5461 agataaaata tcaaccgcaa aaactcacca ggaactcgat gaggtggaag ataaaatttt
     5521 agaaaatata aaaaaactta taaaagaaag tttaaaagaa attaaaacac caagggaaga
     5581 aattaaaaaa attaaagata taacagaaaa ttttgaatat tcttatgtgc taattaattg
     5641 gatagacaaa aaatttaacc caaaaacaga cgaagaactt atagataaat tatttgaact
     5701 tcaaaccaag ccaaacctta tgaaacaagc tcttgaagaa tctaccaggg ttgaattggg
     5761 aatgaaaata gctgatcctt atgatttaat aaagcaaatc agggcagaaa attatcgtgc
     5821 agagtcaaag ctgaacaagg caattttctc tgatttaatt ggtacccaat acgatcttta
     5881 taacaataca aaatcccccc agcaaaaatc agaacaatta tacagatcac tttttatcgc
     5941 catgtcttat ttagataaaa aaaccatcaa aaaatataaa gaaatgtttt ttaacaagta
     6001 tcaagtcaga ccggcatttc ctgaaatacc tgtaatagat aaagaagaag ttaacaaagt
     6061 aaataaggag tgggaagatt tctttataga aacagttcaa actatttctg atatgaaaaa
     6121 atttgccaaa ttagaggaat tatatcaaca agcacaggcg ggagcagata ttattagaaa
     6181 taattacaat aaagaatctg taattgcact atatccgatt ttaaatgaaa tgaaaaacgt
     6241 ttttgcagat gaagatgaag aaattaaaaa tattttagat aatattatta aatctaaccc
     6301 aaaagatttt attgcaaatt ttgccgaatt aaaaaaaaca atggaagaaa ttcaaaaaca
     6361 attacctcaa aaagatatga aaaaattaat tggcaattca attaaaaaca ctaacaccag
     6421 ctgcgaaatc atgataaaaa gttatataca gcccaaacat caaagtcaaa tgaaggaagt
     6481 cataaataac cttataagat ctctttataa aaatgaaaat agaaatgcac cggaaaatca
     6541 acatgcgtta aatcttttta ggcaaaatct tgaaaaatgc agtgtgctga ataatcctat
     6601 tgatatattg cacaacattt taaatatttt agaaaatcct tcacaaaata aatccgtaaa
     6661 tttaagcgta atgacaaatc ttaaaaatta tgccaataga gcctttagag caagtgattt
     6721 aaccgaaata gaatacaaaa taatgaaaaa catacaaaac ggtaatataa caagatttaa
     6781 aagacttatg ggtagagatt acttagtcgg tcaagataaa accaaattca aaatggactc
     6841 aaaagacggt cttggaacta tattattaaa cctgttagat gaaaaaaatc aaaacaggac
     6901 tttaaaactt tttgttgaat ctctcgggct taatgaagaa atagtatcgt ttttcaatca
     6961 agttattaaa cctgaagaac agttaaaaag tcttttagat actattcaag aagcaaaaaa
     7021 tgcaattaac gacagaaatc ttatcaaaga ggttttaaca aaaggcttaa acgcaaaaga
     7081 catgtcaaat atcccctcac aggtttttga acaaataaaa gaaaaactta ctaaaaataa
     7141 tgttggcgaa gaaaaaacta tttcaaaatt tttacaaata tactgccggg ttatggaaga
     7201 aaacttggcc gccaataaac agcctttctc aagaaacgaa gttattaatg ttgttaacaa
     7261 agcccatata aaggccttca ataaggcata cgcaaatttt caggaaaatt tggaagacat
     7321 aaacaccaat tttacagact tacaaagcat agaagaaata attactacat taagcctgaa
     7381 tgaagactct gaaacttaca aacaagctca aaattatcaa caggaattaa ttaaagttat
     7441 tcaaaaaatt gcaaaagaaa ttgaaaaact taacagcatg actccggcag aagaagagga
     7501 agaataagaa ttgtaaaata taaaaaatct ttaaatttgt aaatttttgt atttttccat
     7561 cccaccaaaa gcaattttta ttttcataag actttatagt cttgtgtaaa gggttttcta
     7621 atgccgtcaa taaattcaaa ttacgacaaa aaaataagct ttacggggct tacaagttac
     7681 ctgcgaaaaa cagtttttcc ttgcgatgat gtaaaaaaac ttgcaacgga tatttttgac
     7741 aatagttttg cagggaattt gccttccgaa tggattgaga aaatacctaa aaatcaaaga
     7801 acgcaaacca ttaaagatgt ttttcaaggc tttagagaat ttgtacaact tattaatgaa
     7861 aaatccgaaa ccgctttaag ccagttagaa aaaacactta aaggtgccgg aattttatct
     7921 tatgacaaaa gtattaatct aaaatttgag ggaagcggct cttacgggag agtttatcgc
     7981 cttgaaggct tggaaaacca aaaaaccggc gaacagtatg ttataaaact atataacaaa
     8041 gctcagaaaa atgcccaaaa caatggaata tttgccgaat taaaccgggg cgggctttat
     8101 tggcccgagg ctcttggcaa aaaaacccaa atggtaaaaa catttttcgc agatttagaa
     8161 tcaggctttg tagttaataa atttatagac gattctcttg cctctccaaa aaatattgta
     8221 ccatcaaagc ttttagggct ggaaagctat gatgaccatc caaaacaaaa tgttttaaat
     8281 aatttatatt cgctaaatgt tattaacgga tatgtaattg atcaaggctg cctaagagcg
     8341 gtctgtcctg aaattatcaa cagcaaagat gccagatatg ttataaaaaa gattttcttt
     8401 accccaaaaa ataaagctta tgaaaaatgg cttgaacttt taaacaaccc tgcctataag
     8461 cacaattcca gcgtgaaagt tgcccttatt gaagcaatgg aatacttaca gccaggggac
     8521 aggacaaaat gttttaatga aatattggaa cgttttaata aagaccaaaa attaaagcat
     8581 tatttgacaa aaaatataaa attcattcag gatgaagatt ttgccccttg ttttgcaaaa
     8641 ctttggtcac aatctgaacc tgttgaaaaa aatgaattaa taaaaaatat tgacagatta
     8701 cctcaaaaaa tgcaaaaaaa ttatatttca gaagcgataa aagaaaaaac tctcattcct
     8761 gaggttgtag aaaaatttga caatttacca aaagaagaac ttgccgattt attcaactcg
     8821 gcaattaaag aatcagatga tgaaagtaaa ataaaacttt ttgaaaaatt atttttactg
     8881 cctgaggagc tgcgcctgga atatgctaaa aaaattctac aaaacattga aaatatgaat
     8941 attcttggca aagttgaaga aaaaacctat cttttaaggc ttaacggctc gcaagattat
     9001 aaaaaagctt atgagttttt aattacggat gcaaaagata caacaaaagt attcataata
     9061 agcaaggttt taaaaaatct tgattttgaa gattatgtga aagccgttaa actgttggca
     9121 aaagattcta gtgaagccgt aaaatttaaa ctagccccga cacttgaaaa aataatcgaa
     9181 aacaaaagcg caaaaaggga agatttagac tttatttacc aagagttagc taaaaacaca
     9241 aataatcaaa attatttgga ttatataaaa gaagtctttc agcattagtc tttcaaaacg
     9301 ttaatataaa ttccaccttt aatccttttt aaaatgcccg attgggggtt tattatttgt
     9361 aatactgatt taaattaata ttaggaggaa aaaatgcctg agcctttttt taatgaaaaa
     9421 aaagatacct ttaacaatat aattaatgta attcaagaaa cacttgacgt aaacaaaact
     9481 aaaaatataa ttatagaaac tatttgcaaa accttcaatg caaacagatg ctgtattgta
     9541 gattatgaca aaaggctcaa caaattttta cctattgaac atgaatatat atcttcggac
     9601 aaagtttcaa gctgcagggg acaagatgtt caaacaattg tacccggtct tgtagaagca
     9661 gcaaaaaaag gcaaatttat tcttgttaaa aataaagaaa tattttttga cacagacaaa
     9721 caagttttac aaaccgaaca agatacattt gaaaaatatg atataaattc tcttcttgtt
     9781 ctgccattgt tttaccaaaa cgagcttctg ggaattttgt ccgctcatta taccaatggt
     9841 cacgaaataa ccgattctga aataaatata ataaaaaaca ttggaaatca agcggtattg
     9901 gcaattcaca gagcaaattt atacgaaatg aatcaggctc agcttaaaaa ggcagaagca
     9961 acaaaaaaaa taattgaagc ctcaagaaag tctttggaat tggaggtttt actatctgat
    10021 atatgcaagg aattagctga attatttaaa tttcaaagag ctactatcgg acagcttata
    10081 cgcgaaacac ctgaagaaag tgttgccgta ggagaataca tttacaacaa aaacataaaa
    10141 agctacaaaa gccattctgg ttttggcgaa ataatcaaat attggtcaag ctacttaatg
    10201 cgtgaaaaga aaacaaaaat agtaaatgac atcagcaact ccgatatgcc tgcaaaggtt
    10261 gcgcaaatat acaaagaaat cggcattaat tctattgcct gtattcccct gagaagtgaa
    10321 gaaaatatga tttggggcgg aatgttttta tcggaaaaag aagataatag aaatgtaatt
    10381 gaagaaaatc ttgaattttt agaaacagtc ggctcacaaa tttatatagc aatccgtcag
    10441 gcagagttat ataaaaaaga aaaagaagca cttgagcgag aatctctttt aagaaaaata
    10501 acatcggcaa taagaagcac tttagacata gatgaactgg aaaaaataat agttgaaaaa
    10561 atagcaaaag cactaagggc agaccgctgc tttattatag catacgatga tgaaaccaat
    10621 aaatttttgc cggtaaaaac ataatatgta ctttcgcctg aagtaaaaag tatggtagga
    10681 tttgacattg aaaaagagct tcctgatttg attgccaaaa ataaaaataa tgaagatgtt
    10741 ataattaccg atttagataa atttttggcg cagaacaact tttacaacac cccgattgaa
    10801 aaacatttca gagaatataa aacccaatcg ggatttgcca cgtcaatatt ttattcacaa
    10861 aagcttttag gagttttagc aatacattat ttaaataaga aaggcaaatt tatcgaagaa
    10921 gaaattcagc tggtaaaagc tcttgcagac caaataggaa tagcaattaa tcaagctcgt
    10981 ctttacaaaa gcgaaaagga agcaaaagaa aaaatgagca gcagcataat cgaaataatg
    11041 agaaaatctt tagataaaac catgattaaa aaacttttcg taaaaaatat cagaaaattt
    11101 tttaaagcag atagggtttt tctgtctgaa tatgatgccg aaaaaaagag atacttgcct
    11161 gccgataaag attcggaata tttatcaaat tacggcgaaa aaagctttgt tcaatacgcg
    11221 tggaataatc ccgaagaact tgaatatatt gaacctctgc tgaacaaaga agaattgatt
    11281 atatacaact gggatgagta tattaaacaa aataaaaaaa gtcccggttt tataaaactg
    11341 tttgaagatg ccggtgtaaa atcaagttat aattttccca ttcttcatta tcaaaatata
    11401 atgggactgt tatgcatgga atttaccaca gaagtcgtca ggttgacaga agaagagctt
    11461 aatataataa gaaacatctg tacacaggtc ggaatagcac tatatcaagc gaaattatac
    11521 ttagaggccc agcaatgctt tttttcaaaa gaacagttgt tggaaaaagt ttcggaaaaa
    11581 atcgaaccgc ctttaaagac cattttacaa gaagtttcac atttatcaga aaataatacg
    11641 gtacgtgaag agcaagtaaa caatataatt gataactgca gcttgataat gaactcgaca
    11701 aaagaggttt cacaagaaat ctaatctgat taaatatcaa aaataggctt atatgtcata
    11761 tttaacacaa ctgcagtact ttaaattttt tgaggttata agatatttta ctttaatgaa
    11821 gaagccggct cacacgactc tttttttgta tttttgtctg tttcttggtt atcttcctct
    11881 gaaagaaaag ctttaagttg agcttcaaat tgttctgttg caccgtctaa tacactttgc
    11941 aaatccgata atgaagaata aattttattt gcttcttcct tggatttttc agcaagtgat
    12001 tttacctctt ttgcaacaat atcaaacccg gcaccaattt ctcctgcatg agcagcttca
    12061 attgaagcat ttattgacaa aatagatgtc tggaaagcta ttccttcaat tgcttttact
    12121 attggattga atttagccag caatttttct gcgttaaatt cattaagtat taaactttgg
    12181 aattcagaca tagcgtttct tcttttttcg gaatttgtct caagcatttt atgctgataa
    12241 taatggcaat tatgaggatt attcaacccg ttataaatag ctattgccat atccttacag
    12301 tttccgtaac cgcatgccga acaattttta tgatcttcgg gagtatattt atgcatagca
    12361 tgataaattt cttccaaagc actgtttggc acttgggtta ccagtttatt ttttgagcga
    12421 tcaacataat ttctatcata aagaccgggt ttccagtgtt cttcaataga atcttttata
    12481 cgctcttgta taatttcgtc tttatccact tcagacattc cggaagtatt ttgcccatct
    12541 aagtataatc tttgcatttg ttgtcttctg gcttctatca aactttcaag tgcccccgtt
    12601 gacagttctt tacaatctgt gccggtgcct ccgttacaac ctttttcgca attcaggcaa
    12661 tccactattt gtcaagcccc caatctttgg tccaaagtta aagttagttt ttgcggattt
    12721 tttagcggca taacttttgc catcggtgca ggcggggcat agtttagagc actatgcggt
    12781 ctaaactcat tgtataatct tctgtaatct tctaacatca cttctgcctc cttgattgtg
    12841 taaaacattt ctcc
//
LOCUS       DAOQAU010000049        12759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5449_length_12759_cov_9.097607, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000049 DAOQAU010000000
VERSION     DAOQAU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_12759_cov_9.097607"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKI94_07035"
     CDS             complement(<1..491)
                     /locus_tag="PKI94_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26524.1"
                     /translation="MKLRSLLVSLILMMTLAMPCFADPSGKIVYPGGPLIPVGGTISL
                     HKIDAKYISAGLAPIKPIKPILPPVVPPILPPVVPPVVPPVIPPVVPPVDTSVTGNLP
                     STETIKTEQFIPKQSQLSQPAMAARTNVTLAGAAAQIHHEQNVILVGYKDNLLLDRGV
                     WNST"
     gene            592..2394
                     /locus_tag="PKI94_07040"
     CDS             592..2394
                     /locus_tag="PKI94_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNW26525.1"
                     /translation="MKQKLTFKRVALILIFFLIQNPVFSASGAPVNSTIDPGVINEIN
                     RDELKYQRLKDSVKRGGDSNDIKEAVVQKTYENGKGMVINPSIGLNKVTFAGNTIFKE
                     KELEKYFSHLLGKDIYLSDIFAAIDEINKMYYTNGYYTSYAYIPAQRIENNSIVINIV
                     EGKIGEINIEGNTRSKDSYIKNCFLCANGIEEGKVFNSNDIRKTMDTINSKSYIQGRI
                     SVDEGKTPNTSDIKLELAERYPLGFNVTWDNGGNRLVGRQRSIMLLSQDNLFGLGHSI
                     YGGSVLSKGTTGALAGYKMPIGKHGTELQFDYSFARVNLLDEYAANDIKGKAQTFSTR
                     IVQPLYKTANTDFKTDLGIDFVGANSMEYATNTQLSNYKLTVLRHGLNLLKYDNSGLF
                     LSRFESSFGLPILGATESTGSYFNNDTTNAQSVFFKIKLDLTRLQQLPKDCYGIFRIS
                     SQYTPNNLFPAEQMYFGGVGSIRGFEPGSVLGDIGINGTLEVRTPVPYLRLLLPKKLE
                     KYDKKVRLGFFYDWGIFRQQNSGVAMQTSSNLLQSVGSGIHFNVTDSVIASFEVGIPV
                     SGSLYREQSARFHFSIRADLWDLFTKKPKIQQQL"
     gene            complement(2396..3085)
                     /locus_tag="PKI94_07045"
     CDS             complement(2396..3085)
                     /locus_tag="PKI94_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNW26526.1"
                     /translation="MKKFLFYLLILFCFIAFQGLPVNAKNYQESLLADFNYRLSLPQT
                     DVAQSQWYNNSSLDNWGPWPKRYPSLDNSILSKIPAGTNVTQWKRDRVVAVAKYYVGL
                     PYMHHHIPAWNPKIPDKSGKIGYGLDCSNFSAWVYNIGFGIMLNSNVRKQAQMLPATN
                     FNTAQPGMKKIERGQKLYPGDLLFIGSGPSHVAIYINENEVIDSIGTGVKIRPFSGSY
                     KNYVFAVRIFN"
     gene            3253..4209
                     /locus_tag="PKI94_07050"
     CDS             3253..4209
                     /locus_tag="PKI94_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNW26527.1"
                     /translation="MDYCIHETYFFRNIETLEFIKDYILKTFPKGTNIADFGCSNGEE
                     AYSLGMLLSRQNKNKKYKITGYDLSQKVLDLAQSGPFEKAGGYSELTISNDSYYEKSD
                     RKYLKDIFFECFNKLPAKYIDFNYTDEGKKLLDERIKNQTDLEKLLILKCIKNMMSNY
                     HKYDSGNIYLPKAVFIKGVFDFKQGDINDLSQILKPDGKTGVIIFKNAWYHVTGSRHT
                     YALDEIDLEKAEKIMKAAHKILPENGILVVGNLQNDHLYSNNQGRYFFQDGKRIRVCD
                     DTPFHNALRKSGFKPVFYEQIKDKYDENIKKEIYLPSVWQKV"
     gene            complement(4210..5187)
                     /locus_tag="PKI94_07055"
     CDS             complement(4210..5187)
                     /locus_tag="PKI94_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW26528.1"
                     /translation="MNILDVKNLSLGFNTEAGFKQALFDVSFFVEKGKTLAIVGESGC
                     GKTISALSILKLLPKTAKITSGEILFRKEETGNEQQIINLLNLSEKEMQKIRGAKIAL
                     IPQDPMTSLNPLYTIENQLLEVIEKHQNLKGNQAKQKAIEALDAVQIPCAKERLKSYP
                     HEFSGGMKQRAIIAMALACKAEIIIADEPTTALDVTIQAQIMKLLDEIKKEFNTSIIL
                     ITHDLALVEEVSDKICVMYAGRIVEQASTIDFFKSPRHPYSKALLKSVPSKRGEKLAI
                     IKGQPPSIQQDISGCKFHPRCEFAMPTCSEFIPPLKEVSKEHFCACLLE"
     gene            5352..6218
                     /locus_tag="PKI94_07060"
     CDS             5352..6218
                     /locus_tag="PKI94_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26529.1"
                     /translation="MNLQKTFKNISYKLGSSNDSDIATASLAAFKLFFIPVATMSDKK
                     ATKEQKEYAATRDFLTELIALGTYVGVTREFKRHGTTPLCLSYYKKKAKLIRDGKIPG
                     VDPKLFTEEDFKAIENIDKKSFKSVMIDINHRSPEKRIATAEEKKYVDGLTNVVKKFK
                     KAFPPDPVKPEGRIAKIKYNWKNLFNKNLSIQLPKDLYKNVKLNISQMVIWTLAVIVI
                     PPMCNALIKPAMKKFQQMQEKRNPKFNAPVKLDPYPSHNTQMYEKNKTGNNVFGTIYG
                     KTYNNGNMRVGI"
     gene            6215..6778
                     /locus_tag="PKI94_07065"
     CDS             6215..6778
                     /locus_tag="PKI94_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26530.1"
                     /translation="MITAAMSSPIFSDAVQNSVFQVLSEGSLKTVARPLFTLVDKNGT
                     PESRKYSAVKEFVFQTLSLLFYFVLVRSLVEQKGYKLIKKVLENKPDFKILKNHATRD
                     SFMEARSLALKSGIESEEAKFIHPKGAIEALNILGSGIILTILAPKVVMQMVHPTMKQ
                     VDKLFNKEKAAKEQKNGDVFQKSETKS"
     gene            complement(6768..7757)
                     /locus_tag="PKI94_07070"
     CDS             complement(6768..7757)
                     /locus_tag="PKI94_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW26531.1"
                     /translation="MSNCSISVIIPVFNSGKYIEKCLESIINQSFEDMEIICINDGSK
                     DNSLEILEKFSDKDSRIKVFSQKNQGLSASRNRGLELASGKYIIFVDSDDWLKKDFAQ
                     KLFCEIEKNETDFSICAAQAYDCETLQKSDFMSDYLSLSCLAFFENKVFNQFDTKKQM
                     FYIPVMAWGKIYNRDFLKRTKARFEEVLFFEDNLFFYHIYKHAEKISILKEKLYNYRV
                     NNKNSMFQQSKNIFDVKIETLDKIEETIKSFEYFEQMKNDFLKFKLLHMINILNNTSG
                     LNKIKCYQKLQKKFINVDFKIYDENLFKNTPLYKYYDTIRNKNFIKGLCKLSF"
     gene            complement(7750..8820)
                     /locus_tag="PKI94_07075"
     CDS             complement(7750..8820)
                     /locus_tag="PKI94_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26532.1"
                     /translation="MEKCLISIIIPVFNSEKHLVQCLESVINQTLTDIEIICVNDGSK
                     DNSLEILNKYATKDKRISVINTENKGPSHARNKGMEIAKGKYIQFVDADDWIVKNLCQ
                     TVFENAQKNDSDIVIFNGNSFDNNKKITQPKKFFDLLMWENHVNENSKHTFKDYEGSI
                     MSGNLSVWNKLYKKEFLQKNQIIFPENISGIEDYYFHLNTFLNAQIITLIDKYLYNYR
                     VIQSFTITSHFAKSDNIFQTFALADSIDELYKKKRFDEIYKYVLLEFKCHLLHFWYIK
                     CAWKNKKKFYKKCREYLINIDLKNYDLFLLSKSDYFQIYDDFISNNFLTYYFKNLILD
                     CKFLNLRAKLNFIKKLTGALYE"
     gene            complement(8831..9634)
                     /locus_tag="PKI94_07080"
     CDS             complement(8831..9634)
                     /locus_tag="PKI94_07080"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNW26533.1"
                     /translation="MKSDFLELEKIIKEKSESKPIYYIPNPGNWGDAIIRYATLKFFK
                     DTNIKYEEIKNISLKIIIKAFFKGATFIYGGGGAWCNCWNHSQHVVNKILDLKKICKK
                     INVIILPSTFENTYDLPEVTVFVRDKFESKENMPQGIFCHDMSFYIDKMNVLPGDEIG
                     YLFRTDKESSGSIIAPKENIDISSMGNELSDVNHLYKFLSKYSVIHTDRMHIAIAGCH
                     LQREVHLYQGGYFKNKAIYQSSLKDNFDNVFFHEEFCLKETVNPPFLMK"
     gene            complement(9657..10376)
                     /locus_tag="PKI94_07085"
     CDS             complement(9657..10376)
                     /locus_tag="PKI94_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW26534.1"
                     /translation="MINLNQHNLEKLFLNNVKLSKTIPIKIKFDKINLRKKGFTLAEV
                     LITLGIIGVVAAITIPTLMSNTGEKRLTSQFMKSYSSVTQALNKWGVDNQCIDRKCLI
                     SAYLADVAGATSEFNSYFNILKETDDGGLHWYTTADGSQIALYADSWTNYEPRVYFDI
                     NGAKLPNKLGIDRFAITVKGLKGADPSYEPIAGPYTLSDCVDLVKYGDQNWFQFVCGD
                     NVLKEKENYYKALREANISLQ"
     gene            complement(10445..10867)
                     /locus_tag="PKI94_07090"
     CDS             complement(10445..10867)
                     /locus_tag="PKI94_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26535.1"
                     /translation="MKYAKFAITLAVLATCTGMAFADGAFTPLNFDDAGYSAPKTTSN
                     PIKSLLNKEGANALQTVPGEVPIGNENIQNAILELDNAQVGIRNDLLNYKSKYADVDA
                     QYKLIKNERSMLYKQVRSIEKRIKDIDRAKDKIRKNML"
     gene            11538..>12759
                     /locus_tag="PKI94_07095"
     rRNA            11538..>12759
                     /locus_tag="PKI94_07095"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4248 a         2121 c         2289 g         4101 t
ORIGIN      
        1 gtggaattcc atacgccgcg atcaagaagg aggttatctt tataaccgac taaaataaca
       61 ttttgttcgt gatgaatttg tgctgctgca ccggcaaggg taacgtttgt tctagctgcc
      121 attgcgggtt gggaaagttg tgattgttta ggtataaatt gttctgtttt tattgtttct
      181 gtggaaggca aatttcctgt tacacttgta tcaaccggag gcactaccgg agggattaca
      241 gggggaacta ccggaggtac cacaggaggt aatatcggag ggactacagg gggtaatatc
      301 ggcttaatag gcttaatagg tgcaagacct gcggatatat attttgcatc tattttatgc
      361 agagatatag taccgccaac aggaattaaa ggaccgccgg gataaactat tttgccacta
      421 ggatcggcaa aacaaggcat tgccaaggtc atcatcaata ttaaacttac taacaaactt
      481 cttaatttca tcttatctct cttttttgct taatgattag aaatatttaa acatttatct
      541 tataatataa taaagtaaat aaataaagtc aaatttcaag aacgataaaa aatgaaacaa
      601 aaattaacat ttaaaagagt agctttaata ttaatatttt ttttgataca aaatcctgtt
      661 ttttcagcct cgggggctcc tgttaattct actatagacc ccggtgtgat aaacgaaatt
      721 aacagggatg aacttaagta tcaacggctt aaggattctg taaaaagagg tggcgattca
      781 aatgatatta aagaagccgt cgtgcaaaaa acttatgaaa acggaaaagg aatggtcata
      841 aatccttcaa taggactcaa taaagttact tttgcaggaa atactatttt taaagaaaaa
      901 gaacttgaaa aatatttttc gcatttatta ggaaaagaca tttacctttc ggatattttt
      961 gcggctatag atgaaattaa caaaatgtat tacacaaacg gctattacac atcttatgcc
     1021 tacattcccg ctcaaagaat tgagaataat tccattgtaa ttaacatagt agaagggaaa
     1081 atcggcgaaa taaacattga aggaaacacc cgttcaaaag acagctatat aaaaaattgc
     1141 tttttatgtg caaatggaat tgaagaagga aaagttttta attcaaatga tatcagaaaa
     1201 accatggata caatcaattc aaaaagctac atacaaggca gaatttctgt tgatgaaggt
     1261 aaaaccccaa acacttccga tattaaatta gaacttgccg aaagatatcc gctggggttc
     1321 aacgtaactt gggacaacgg cgggaacagg cttgtaggtc gtcaaagaag tattatgctt
     1381 ttatctcagg ataacctttt cggcttaggt cattcaatat atggaggctc tgtcctttca
     1441 aaaggcacaa cgggagctct tgccggatat aaaatgccca taggaaaaca cggaacagag
     1501 ctgcaatttg actattcttt tgccagagtt aatttattgg atgaatatgc ggcaaacgac
     1561 attaaaggta aggcacaaac tttcagcaca aggattgttc aaccgcttta caaaacagca
     1621 aatactgatt ttaagactga tttaggcatt gattttgtcg gagcaaattc tatggaatat
     1681 gccacaaaca cccaattaag caactataag ttaactgttt taaggcatgg cttaaatctt
     1741 ttaaaatatg acaattcagg tcttttctta agcagattcg aatcaagctt cgggcttcct
     1801 attttaggcg caacagaaag caccggaagt tatttcaaca acgatacaac caatgcacag
     1861 agcgtgtttt tcaaaataaa attggacctg acaagacttc agcaattgcc aaaagactgc
     1921 tacgggattt tcagaatatc ttcccaatac acacctaaca acctgtttcc ggcagaacaa
     1981 atgtattttg gcggagtagg ttcaataaga ggtttcgagc cgggaagcgt gttaggagat
     2041 ataggaatta acggtacttt agaagtcaga acacctgtac cttatttaag acttttactt
     2101 cctaaaaaac ttgaaaaata cgacaaaaaa gtacgactcg gatttttcta tgattggggt
     2161 attttcagac aacagaactc tggagtagca atgcaaacgt caagcaatct tctgcaaagc
     2221 gtaggaagcg gtattcactt caatgttaca gattctgtca ttgcatcttt cgaagtcgga
     2281 attcctgtaa gcggaagtct ttacagagag caaagtgcaa gatttcattt ttcgataaga
     2341 gccgaccttt gggatttatt tacgaaaaag ccgaaaattc aacaacagtt ataagttaat
     2401 taaaaatcct tacggcaaaa acgtaatttt tataactgcc tgaaaacggt cttattttta
     2461 cgcccgtacc tatagaatca attacttcat tttcgtttat ataaattgcg acatgcgaag
     2521 gtcctgagcc tataaataaa agatcgcccg gataaagttt ctgccctctt tcgatttttt
     2581 tcatgcccgg ctgcgcagtg ttaaaattag ttgcaggaag catctgggct tgttttctaa
     2641 cattgctgtt aagcattatc ccaaagccta tattataaac ccacgcgctg aaattactgc
     2701 agtcaagtcc ataacctatt ttgccggatt tatcagggat tttcggattc catgcaggaa
     2761 tatggtggtg catgtaaggt agtccgacat aatactttgc aacagcaaca actctgtcac
     2821 gcttccattg agtaacattt gttcctgcag gaatcttgga taaaatacta ttgtcaaggg
     2881 aaggatacct tttgggccaa ggtccccaat tatcaagtga agagttatta taccattgac
     2941 tttgagcaac atctgtctgc ggcagagaaa gtctataatt aaaatccgcc aaaagggatt
     3001 cttgataatt ctttgcattt acaggcaaac cttgaaaagc aataaaacaa aataaaatta
     3061 ataaataaaa caaaaatttt ttcatgtaaa aattatagaa caaaagttta caaataggca
     3121 aaaaaaaaat tgttcggatt tcatttaaac atgatatatc ctattcaacc ttacaactta
     3181 aataattatc gccataaagt atcctttaga ggctacaata atgatacata tcatgtaaaa
     3241 tcaaaaaaca aaatggatta ttgtattcat gaaacttatt ttttcagaaa cattgaaact
     3301 ttagaattta taaaagatta tatcttaaaa acttttccaa aaggcacaaa catagcagat
     3361 tttggctgct ctaacggaga agaagcttat tctctgggca tgcttttgag cagacaaaac
     3421 aaaaacaaaa aatataaaat tacaggatat gatctttctc aaaaagtact tgatttagct
     3481 caaagcggac cattcgaaaa agccggtggt tattcagagt taactataag caacgatagt
     3541 tattatgaaa aaagtgatag aaaatacttg aaagatatat tctttgaatg ttttaataaa
     3601 ttaccggcaa aatatattga cttcaattat acagatgaag gcaaaaaact tttggatgaa
     3661 agaattaaaa atcaaacgga tttagaaaaa cttttaattt taaaatgtat aaaaaatatg
     3721 atgtcaaact atcataaata tgattccggc aatatatact taccaaaagc tgtttttata
     3781 aaaggagttt ttgattttaa acaaggtgat ataaatgacc tttctcaaat actaaaaccc
     3841 gacggaaaaa caggggtaat tatttttaaa aatgcctggt atcatgtaac tggctcaagg
     3901 catacttatg ccttagacga aattgacctg gaaaaagccg aaaaaatcat gaaagcagcg
     3961 cataaaattt taccggaaaa tggtattctt gttgtcggaa atcttcaaaa tgaccatctt
     4021 tacagtaaca atcagggaag atactttttt caagacggca aaagaataag ggtttgtgac
     4081 gatacgcctt ttcacaatgc acttagaaaa agcggattta agcctgtttt ttatgaacag
     4141 ataaaagata aatatgatga aaatataaaa aaggaaattt acttgccctc tgtttggcag
     4201 aaagtttaat tactccaaaa gacaagcgca aaaatgttct tttgaaacct cttttagagg
     4261 cggaataaat tcagaacatg tcggcattgc aaattcgcat cgcgggtgga atttacagcc
     4321 tgaaatatct tgctgaatag aaggcggctg ccctttaata atagcaagtt tttcgcctct
     4381 ttttgaagga accgatttta aaagtgcctt tgaataaggg tgccttggac ttttaaaaaa
     4441 gtctatggtt gaagcttgtt caacaattct tccggcatac attacacaaa ttttatccga
     4501 aacctcctca acaagtgcca aatcgtgagt aattaaaata attgatgtat taaattcttt
     4561 tttaatttca tccaaaagtt tcatgatttg tgcctgaatt gtaacgtcaa gtgctgttgt
     4621 gggctcatct gcaattataa tttctgcctt gcaggcaagt gccattgcaa ttatcgctcg
     4681 ctgtttcatt ccgcctgaaa actcgtgggg gtatgatttc agacgctctt ttgcacaagg
     4741 aatttgaacc gcatcaagtg cttctattgc tttttgtttt gcttgatttc cttttaaatt
     4801 ttgatgtttt tctatgactt ctaagagctg gttttctata gtgtataaag ggtttagaga
     4861 agtcatgggg tcttggggaa ttaaggcaat ttttgccccg cgaatttttt gcatttcttt
     4921 ttcacttaga tttaataaat taattatctg ttgctcgttt cctgtttcct ctttcctgaa
     4981 taaaatttcc ccgcttgtaa ttttagcggt ctttggcaga agcttaagga tgctaagggc
     5041 acttattgtt ttcccgcaac cggattcacc cacaatggca agggtttttc ctttttcgac
     5101 aaaaaacgag acatcaaaaa gtgcctgttt aaagcctgct tcggtgttaa aacccagtga
     5161 taaatttttg acatccagta tgttcatttt ataattatat atgttaaacg aaaaaataac
     5221 atagccgaat gagcattaaa tatctttttt gacttgggag attgttaatt tttgttaaaa
     5281 aaagaattaa aatagcaaat tttattttta gctactttaa taaacatgta tttcatagac
     5341 aggttttatt aatgaactta caaaaaacat ttaaaaatat atcttataaa ctgggctcat
     5401 ctaatgattc agacattgca acagcttcat tggctgcatt taaactgttt tttattcctg
     5461 ttgccacaat gtctgataag aaagcaacaa aagaacaaaa agagtatgca gctacaagag
     5521 attttttaac agaattaatt gccttaggaa catacgtagg agttacaagg gaatttaaaa
     5581 gacatggaac aacaccgctt tgtctcagtt attataagaa aaaggcaaag cttatcagag
     5641 atggaaaaat ccccggtgtt gaccctaaac tttttacaga agaagatttt aaagcgattg
     5701 aaaatattga caaaaaaagc ttcaaatcag ttatgattga tatcaatcac agaagtccgg
     5761 aaaaaagaat agcgactgct gaagaaaaaa aatatgtaga cggtttaacc aatgttgtca
     5821 aaaaatttaa aaaggctttt cctccggatc ctgtgaaacc tgaaggtagg atcgcaaaaa
     5881 taaaatacaa ctggaaaaat ttatttaata aaaatttatc cattcaactt ccgaaagatt
     5941 tatacaaaaa cgtaaaatta aatatttcac aaatggttat ttggacttta gcggtaattg
     6001 ttataccgcc tatgtgtaat gccttgataa aacctgctat gaaaaaattc caacaaatgc
     6061 aagaaaaaag aaatccaaaa tttaatgctc ctgtgaaact ggatccttat ccaagtcaca
     6121 acactcaaat gtatgaaaaa aataaaaccg ggaataatgt ttttggaaca atttatggaa
     6181 aaacttataa caacggaaat atgagggtag gtatatgata acagcagcaa tgtcaagtcc
     6241 tatattctca gatgcggttc aaaactccgt atttcaagta ttatcggaag gctctttaaa
     6301 aacagttgca agacctttat ttactcttgt tgataaaaac ggaacccccg aatccagaaa
     6361 atattctgct gtaaaagaat ttgtgtttca aacattaagt cttttgtttt attttgttct
     6421 tgttcgctcg cttgttgaac aaaaaggtta taaattaata aaaaaagtat tagaaaataa
     6481 acctgatttt aaaatattaa aaaaccatgc aacaagagac tcttttatgg aagcaagatc
     6541 tcttgcttta aaaagcggaa tagaatcaga agaagctaaa tttatacatc cgaaaggtgc
     6601 tatagaagct ttgaatattt taggctccgg tattattttg accattcttg ctccaaaagt
     6661 tgttatgcaa atggttcatc ctacaatgaa acaagttgat aaattattta ataaagaaaa
     6721 agccgcaaaa gaacaaaaaa acggtgatgt ttttcaaaaa tcagagacta aaagctaagt
     6781 ttacataatc cttttataaa atttttattt cttattgtgt catagtattt atacaaaggg
     6841 gtatttttaa aaagattttc atcataaatt ttgaaatcta cattgatgaa ttttttttga
     6901 agtttttggt agcattttat tttattcagc cccgaagtat tgtttaaaat attaatcata
     6961 tgcaataatt taaattttaa aaagtcattt ttcatttgct caaaatattc aaaacttttt
     7021 atggtttctt cgattttgtc aagggtttct attttaacgt caaaaatatt ttttgactgc
     7081 tgaaacattg agttcttgtt gtttactctg taattataaa gtttttcctt caatatcgat
     7141 attttttcgg cgtgcttata aatatgataa aaaaacaaat tgtcttcaaa aaataagacc
     7201 tcttcaaatc ttgcttttgt tctttttaaa aaatctctgt tgtaaatttt gccccatgcc
     7261 ataacgggta tataaaacat ttgttttttt gtgtcaaatt ggttaaatac tttgttttca
     7321 aaaaaagcaa gacaagaaag cgaaagataa tcactcataa aatctgattt ttggagtgtt
     7381 tcacagtcat aggcttgggc ggcacagatt gaaaagtcgg tttcattttt ttctatttcg
     7441 caaaataatt tttgtgcaaa atcttttttc agccaatcat cagaatctac aaaaataata
     7501 tatttcccgc ttgcaagttc aagccctcta tttcttgacg cgctcaaccc ctgatttttt
     7561 tgtgaaaaaa cctttattct ggaatctttg tcggaaaatt tttccaaaat ttcaagagaa
     7621 ttatccttag agccgtcatt tatgcaaata atttccatat cttcaaagct ttgattaata
     7681 atgctttcaa gacatttttc aatatatttt cccgagttga aaacaggaat gataacagaa
     7741 atcgaacaat tactcataca aggctccagt gagttttttg ataaaattca attttgctct
     7801 taaatttaaa aatttgcaat ccagaattaa gtttttaaaa tagtaagtta aaaaattatt
     7861 acttataaaa tcatcataaa tttgaaaata gtctgatttt gaaagtaaaa aaaggtcata
     7921 gtttttcaaa tctatattaa tcaagtattc cctgcatttt ttataaaact tttttttgtt
     7981 tttccatgcg cattttatat accaaaaatg cagcaggtgg catttgaatt ctaaaagaac
     8041 gtatttataa atttcatcaa atcttttctt tttgtagagt tcatcaatag aatctgccag
     8101 agcaaaagtt tggaaaatat tgtcgctttt tgcaaaatga gaagttatgg taaaagactg
     8161 aattaccctg tagttgtata aatatttatc tataagggtt attatttgag cgtttagaaa
     8221 ggtgttgaga tgaaaataat aatcttctat tccactgata ttttcaggaa aaattatctg
     8281 atttttttgc aaaaactctt ttttatacaa tttgttccag acagaaagat tacctgacat
     8341 aattgatcct tcgtaatctt taaaagtgtg tttggagttt tcgtttacgt gattttccca
     8401 cataagaagg tcaaaaaatt tttttggctg agtaattttc ttgttattat caaaagaatt
     8461 tccgttgaat attacaatgt cagaatcgtt tttttgagca ttttcaaaga ctgtttggca
     8521 taaatttttt acaatccagt catctgcatc tacaaattga atgtacttgc ctttggcaat
     8581 ttccattcct ttgtttctgg catgtgaagg acctttgttt tctgtattga ttacgctaat
     8641 tcgcttatct ttagtcgcgt atttatttaa tatttcaaga gaattatcct tagagccgtc
     8701 attgacacaa attatctcga tgtctgtcag cgtttgatta ataacacttt caaggcattg
     8761 gacaaggtgt ttttctgaat tgaaaacggg aatgattatt gaaattaaac atttttccat
     8821 aaacaccaac ttatttcatc aaaaatgggg gattaacagt ttctttaagg caaaattctt
     8881 cgtggaaaaa aacgttgtca aaattatctt ttaagctgct ttggtatatc gctttatttt
     8941 taaaataacc cccttgataa aggtgcactt ctctttgaag atgacaccct gcgattgcta
     9001 tatgcattct gtcggtgtga ataactgaat attttgataa aaatttataa agatgattta
     9061 cgtcggataa ttcatttccc attgaactta tatcaatatt ttctttgggc gcgattattg
     9121 agcctgagct ttctttgtcg gttctgaata aataaccgat ttcatctccc ggcagcacgt
     9181 tcattttgtc gatataaaaa gacatgtcgt ggcaaaaaat gccttggggc atgttttctt
     9241 tgctttcaaa tttatctcga acaaagaccg taacttcagg taaatcgtaa gtattttcaa
     9301 aagttgaagg caaaattatt acattaattt ttttgcagat tttttttaaa tcaagaattt
     9361 tatttactac gtgctgtgaa tgattccagc aattacacca tgctccacca ccaccatata
     9421 taaaagttgc acctttaaaa aatgctttaa taattatttt tagggaaata tttttaattt
     9481 cttcatattt tatatttgtg tctttgaaaa attttaaagt cgcatatctg attattgcat
     9541 caccccagtt accggggttt ggaatgtaat aaatcggttt gctctcgctt ttttctttta
     9601 taattttttc aagctctaaa aaatcggatt tcatatatct ataatactat aaaattttat
     9661 tgaagagaaa tattggcttc gcggagagct ttataatagt tttctttttc ttttaaaaca
     9721 ttgtctccac agacaaattg gaaccaattc tgatcaccgt atttcaccag atctacacaa
     9781 tcacttaaag tatatggtcc tgctatgggt tcataagaag gatccgcgcc ttttaatcct
     9841 tttactgtga ttgcaaatcg gtcaattcca agtttattag gaagtttagc accgtttata
     9901 tcaaaataaa ctctaggttc ataatttgtc cacgaatcgg catataaagc aatttgagag
     9961 ccgtcagctg ttgtgtacca gtgtaaaccg ccatcatctg tttcttttaa aatattaaaa
    10021 tagctattga attcagatgt ggctccggca acatcagcta aataagcact tattaggcat
    10081 tttctgtcaa tacattgatt atcaacaccc catttgttaa gtgcttgtgt aactgaagag
    10141 tagcttttca taaattgaga ggtcaggcgc ttttcaccag tatttgacat taaggtagga
    10201 attgtaattg ctgctacaac ccctattatg cctaaggtaa ttaggacttc tgccaaagtg
    10261 aatccttttt ttcttaaatt aattttgtcg aatttaattt taattgggat ggttttgctt
    10321 agtttaacgt tgtttaaaaa taatttttct aaattatgtt ggtttaaatt aatcataatt
    10381 ctctttctcc attttaaatt taagagctta ggtttttaaa aacctaagct cttgataata
    10441 atatttacag catatttttt ctaattttat cttttgctct gtcaatgtct ttaattcttt
    10501 tttcgattga ccttacttgt ttataaagca tgcttctttc gtttttaatt aatttatatt
    10561 gtgcgtcaac atctgcgtat tttgatttgt aattcaaaag gtcgtttctg atgcccactt
    10621 gagcattatc aagttcaagg attgcatttt gaatattttc attacctata ggcacttcgc
    10681 ccggaacagt ttgcagagca tttgcgcctt ctttattcaa aagactttta atagggttag
    10741 aagtcgtttt tggcgcggaa taacctgcgt catcaaaatt taacggcgta aaagcaccat
    10801 ctgcaaaggc catgccagta caagttgcta aaacggctag tgtgattgca aattttgcat
    10861 atttcatatt tcaatctcct ctcgtaaaat atcgtattac aattatcatt ttattatact
    10921 tttactcaaa aataaaataa gttaactttt gttaataaat atataaacat gctgttgatt
    10981 tattttttag tttaaagtaa gatagtgatt acgatataaa tttacataga acattagaga
    11041 ccgaaataaa agcactaaat atttttcaat ttcagtactt gacaacagag attgaagtaa
    11101 taatattgta aataccgcta tatgcggttc aattttggga gcggccctaa atggcgagag
    11161 cctgccgacc tctgataaaa ccccaaataa gggttttcct atccctttag agttaggagc
    11221 tcggcttgtc cgatacctga ccgaaagagt aaagggtgag gttttttaac caaaaatcga
    11281 acattgaaaa cagaatagct gaaaactaag gcattagcaa agaacgaaat gacttgcaat
    11341 tttgcaagac tttgaaactc tttaatgcaa atgccgaaaa atgacaaaat atacttgttg
    11401 attcaacaaa aaatgattta acggacaagc tcgcagcaat gcgagcgaaa gatgacaaaa
    11461 cgcgaaacat aatgttagca atagtcaaaa tgagcatttt acatctttta gcgagatgta
    11521 ataatcataa ctttctgaca atggagagtt tgatcctggc tcaggacgaa cgctggcggc
    11581 gtgcttcata catgcaagtc gaacgagaag caggagcttg ctcctgtgga aagtggcgga
    11641 cgggtgagta atatgtagga aatctgccct agagaggggg acaacagagg gaaacttctg
    11701 ctaatacccc atatgagcgt agctgaaatg ctattcttga aaactccggt gctctaggat
    11761 gagcctgcat ctgattagct tgttggcggg gtaatggccc accaaggcga cgatcagtag
    11821 ctggtttgag aggatgatca gccacaatgg gactgagaca cggcccatac tcctacggga
    11881 ggcagcagta gggaattttg cgcaatgggc gaaagcctga cgcagcaacg ccgcgtgaat
    11941 gattaagccc ttcggggtgt aaagttctgt cagtggggac gaaacttgac ggtacccaca
    12001 gaggaagcac cggctaactc cgtgccagca gccgcggtaa tacggagggt gcaagcgttg
    12061 tccggaatca ttgggcgtaa agagttcgta ggtggtatgt taagtctggt gttaaatccc
    12121 gaagctcaac ttcggtccgg caccggatac tggcaaactt gaatgcggta gaggtaaagg
    12181 gaattcctgg tgtagcggtg aaatgcgtag atatcaggag gaacatcggt ggcgtaagcg
    12241 ctttactggg ccgtaattga cactgaggaa cgaaagccgg ggtagcaaat gggattagat
    12301 accccagtag tcccggccgt aaacgatgga tactaggtgt tgcgggtatc gacccctgca
    12361 gtgccgtagc taacgcgata agtatcccgc ctggggagta cgcacgcaag tgtgaaactc
    12421 aaaggaattg acggggaccc gcacaagcag cggagcatgt ggtttaattc gaagcaacgc
    12481 gaagaacctt accaggtctt gacatccagt aaaacctgta gagatataag gtgtgcttgc
    12541 acatactgag acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa
    12601 gtcccgcaac gagcgcaacc ctcgtcgtta gttgcacata tcggtacact gatatggtgc
    12661 tctctagcga gactgccggt gataaaccgg aggaaggtgg ggacgacgtc aaatcatcat
    12721 gccccttatg ctctgggcta cacacgtgtt acaatggct
//
LOCUS       DAOQAU010000050        12074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_5931_length_12074_cov_5.874033, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000050 DAOQAU010000000
VERSION     DAOQAU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_12074_cov_5.874033"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKI94_07100"
     CDS             complement(<1..788)
                     /locus_tag="PKI94_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26536.1"
                     /translation="MGLAASQCRLLFLTSRQNDIQKGMQDTTMRRLALTRATQKITRE
                     HNRALDSAVYKTSWQANGNLDVDTMSLNYSKFMTPTGGLGQQFLLTDTSGRVILSDDY
                     CTKLGIAAGTNSGAGSAITKTQSEFLQAIMSINASTANAYIENFNPANDSTTNTSMSV
                     LELLSNEFIDAANSTKQATSASWNLDTLVSSNLSWAEIYNRNLDVLISDRDNQNNALN
                     FKKLLDDFYGDICSRLNINNNSSVALKYAYDQTFRMFSRNQQSVG"
     gene            1016..1258
                     /locus_tag="PKI94_07105"
     CDS             1016..1258
                     /locus_tag="PKI94_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26537.1"
                     /translation="MITQVSGLDSVSVAKDPRRKAQSIKFKHPCSNISYKSGEYRDVY
                     THQKNEALLTSISIVAGSTLFILGSFLFMALKKVKP"
     gene            complement(1291..2664)
                     /locus_tag="PKI94_07110"
     CDS             complement(1291..2664)
                     /locus_tag="PKI94_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26538.1"
                     /translation="MTVISGKPPAKTLGVVIPTAKNPISFSAAETAGIRTEIVNKEEK
                     EKYSAVSNALPKAERKTLEILLKTGKLLNSNSNDKSTTLDNLYKIITEPRAQGLDAAV
                     VLKETVNTIADPFVITQNFGDIPKEYVPKILEIGKNNTLPTNLIQRAMNIQNTKDPIN
                     EQTINVEHSGACVSASIEFNLAKQMPAEFARFAQSLSSPKLSVEKTIHLDKLANTPLD
                     AIWLLNAFEVPYEANDFKTAKLTLAPDKSAITRAQIQNSHKDPLERSLVDVLMQSTFM
                     NVGSQQSYDSLTDVRAGKFNQNDKGLIEFEKTFTESVVEDKNKISVTYQTVDENAKLT
                     GYETDFATIKKHILETLNMGENVIIGYTQTDENNVIINGHEITIIGTKQGKDGKLIFV
                     CNDTDDNYPKQIEYSEDYLIPKIHHAGLPQEVAEKDMQFVDNWKEGLNAYKEMKTQNL
                     GTQIHSA"
     gene            2859..3839
                     /locus_tag="PKI94_07115"
     CDS             2859..3839
                     /locus_tag="PKI94_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26539.1"
                     /translation="MKEKIKIKPLTKSQIIISLDRLTRFKPTEEKYLRVFKDIVLSCL
                     IKPKYNKKQLDAMPYETLKGLAEQIFNSSVSSHSKNPLTINKKLFDYEENIFSLNENV
                     MTLLENKIDYNCAINMIEGEKLPPNLIWLKSLCKNTNQKKYRAEYAIKFPIEKVVITE
                     GITEEILLPNFAKLCGYDFDKYGVYLISAGGKNQVVKLFYSLCENLKLPVFILLDSDA
                     KSNFTQICPKLRKIDKVYIIPKGEFEDILPISLVKKTLNNYLKNFSSIDLKDLKQNKP
                     MTKILEEIFKQKGFGEFKKAEFAQMVKENISGKIALSKDILNIVEQIKNI"
     gene            complement(3836..4393)
                     /locus_tag="PKI94_07120"
     CDS             complement(3836..4393)
                     /locus_tag="PKI94_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26540.1"
                     /translation="MKKSFLIFSLLLISLIFSGAQRSDIYVIDAQKNASMHNNLGAAY
                     MSEQNYFAAIQEYKIAISLNPNSQASAVYYNNLGEAYMMINQSKWAQDCFQRAIKIYP
                     LNFVYYQNLVKCFQAQKCLDYQLKTYLNKPKTSFNTLMIGLIYIEKGQKKQGIIKLDE
                     FCSREPKLIITSAVKDYLNQITQNY"
     gene            complement(4390..5331)
                     /locus_tag="PKI94_07125"
     CDS             complement(4390..5331)
                     /locus_tag="PKI94_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW26541.1"
                     /translation="MRNTKNEFKVKFRGVRGSYPIANNDYLKYGGNTSCVEVNVGGKL
                     IILDAGTGLIELGNLLMQEHIMSGNSIYDRQPVNAILLLSHIHQDHIQGFTFFRPSHL
                     SSTTLNIFGAVDYSQNLAEELSQLLFTKSFPLDLGDIAANLNIFDLNENQVIIIDKDE
                     IKVEKITFRADLKPKNDEIIISCYKSYAHPQEGVMVYKIAYKDKTLVYATDKESYLGG
                     DKKLSMFARNCDLLIHDAQYTTEDYLDPFAPKQGYGHSTFDMALDCQKQSNAKKLAFY
                     HFDPTYSDEKLTEISSHYEGISDSAFLAYEGLEIDLL"
     gene            complement(5342..6325)
                     /locus_tag="PKI94_07130"
     CDS             complement(5342..6325)
                     /locus_tag="PKI94_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNW26542.1"
                     /translation="MKRKNRKRKKESFFKKLTNSLFVVFLAAVIFCGLQVMASADNLS
                     FVQISDAHFSTAKTNTSYRLLAESGEVLEDAINQVNETPNVDFVMFTGDMINTAYEKQ
                     LMQFLPYANKLKCPWYAVFGNHDISIGGYLSKQLYLDILKSHNKNFIFSKQYYSFSPK
                     KGYKVIALDCIIDDRITANGQINEEQLRWLDKELSKSKKDTVLIFLHMPLVEPLNSPN
                     HRILNTDETLAIIEKYKNPIAIFSGHYHATKITQKDNVLHVSTPSLVSYPNAFRLVRI
                     HKQKNKVIFDIYLKETRYKDVQKRAKMMIFGANKYYGADEDRNSTFIIEKK"
     gene            complement(6322..7830)
                     /locus_tag="PKI94_07135"
     CDS             complement(6322..7830)
                     /locus_tag="PKI94_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26543.1"
                     /translation="MKQRLFLILIGLCLFILNPCFALGNEITDDYLDIATNYFNTNNY
                     QKALEYVDEILKIEPQNQCAIDLKNKILPLTGAQTVTAQEEPQAPLTEEPAKVQQQLV
                     GVPDDIEPLKRFEVLNVTSADVEKMDYDSDYYNQKGQEFYAKKDLNVAIEYFFKAINL
                     DKRNAQAYNNLGMCYWLKNNPTLAIKYFKKAHWLNKNYTQPLVNMALLYKQLGCVKQE
                     FYCLQKATKCNPNDYWAYYLLGDYYKDKGMYPKALENYKEAVKINDKFTQAYMALAMT
                     FFETEEFNYTLAALNKYNELNPNSDYSYYMSARTNLILGRYEAAKQDVLKAMEISYSI
                     QYKYELAKIEYYLENYIEALNIFQEVFKTKNSAEVLNYLGLCNYKLKNINEAMSDFTK
                     AIELDGLRPIYYYNLALCYKSMDDKKNYIKYTNSATNILPVSYQDYIDLSYIYFDNGN
                     ASYAINTLNSAIKKYPDVKALYLSKLKIYEVLDDNFHYNEVKEVINTRFNEK"
     gene            complement(7881..8738)
                     /gene="zupT"
                     /locus_tag="PKI94_07140"
     CDS             complement(7881..8738)
                     /gene="zupT"
                     /locus_tag="PKI94_07140"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNW26544.1"
                     /translation="MPHTPANYEIAITMTFLAGLATVVGGMFTFFIKRTNIKALSIGL
                     GFSAGVMIFLSFTEILQGAQKLLQPFYPNNANWMTFFGFTFGMVIAILIDYFIPDHID
                     ENLFGPPEYCKNRKHRHRKENDDENDIICSYKHHKIRRAGILTAIAIAIHNFPEGMAT
                     FFVTTQNVTLGISVAIAIALHNIPEGIAVALPIYHATGKKRMAIWYSFLSGISEPIGA
                     IIGLLVMHYFLPQIAIGFLFAAVAGIMIYISFDTLLPLSREYGDGHYSVFGIMSGIFL
                     IWISLLLIK"
     gene            9011..9376
                     /locus_tag="PKI94_07145"
     CDS             9011..9376
                     /locus_tag="PKI94_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2023 family protein"
                     /protein_id="HNW26545.1"
                     /translation="MLGNFMEVFYHLVYEYQKGLRDLCLFTCVDKFFDKIKKILEYQK
                     IDYLVLALPEDKINIFFGLKDCIDIVKSFSSEELNLLAPEEDFILGMMLGYSRQKQYS
                     RFLKKKNLIEFNQVVAESC"
     gene            complement(9464..10222)
                     /gene="hisF"
                     /locus_tag="PKI94_07150"
     CDS             complement(9464..10222)
                     /gene="hisF"
                     /locus_tag="PKI94_07150"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNW26546.1"
                     /translation="MLAKRIIPCLDVKDGQTVKGVNFENLQYAGDPVALAKKYSDEGA
                     DELVFLDITATNEGRKTTVEMVKKVAKQVFIPFTVGGGINSIEDMRHLLSAGADKVAI
                     NSAAVKNPELIKEASQYFGSQCVVVAIDAKLIDKDFYVFINAGKKNTGLKLLNWAKKA
                     EELGAGEILLTSMDADGTQRGFDLKMTQTIADALNIPVIASGGAGAFISHFVDVFNTA
                     HADAALAASIFHYNTLPIKTLKQELLKNKISIRN"
     gene            complement(10226..11080)
                     /gene="aroE"
                     /locus_tag="PKI94_07155"
     CDS             complement(10226..11080)
                     /gene="aroE"
                     /locus_tag="PKI94_07155"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNW26547.1"
                     /translation="MAYKFALIGSPLTHSVSAVIQKAGFKTLGIEGSYEILETPPDEL
                     ISRLKFLKANDFNGFNVTIPLKVPVSLFLDQVDQYADVAGCVNTVKILPDKSLSGFNT
                     DVYGFKNAIPEDVKPKLKDANVSIIGTGGAARACAVGLIELGVKQISFFSRNIINSST
                     MVNYMRKAFSQTTFKLYQMQSFMELLESKMIVNATPTGMKGHSADKMPLSMQIIHELP
                     TDTIVFDVIYNPVKTLFLEKVSQRGIQTINGLDMLIHQAAKSLELWTGFMPDTDKMKI
                     AALEELLV"
     gene            complement(11070..>12074)
                     /locus_tag="PKI94_07160"
     CDS             complement(11070..>12074)
                     /locus_tag="PKI94_07160"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNW26548.1"
                     /translation="GSSKIIFDGFLKIYCEDEEEICKNSMPEFTKGEVLKCKKVNPKQ
                     HFTQPPPRFSEASLVKALEEHGIGRPSTYAPIITKIQTKGYVEKIEKALAPTILGKTV
                     SKQLVEHFTKIMDYKFTAGMENKLDDVAEKKLIWNEVLKEFYTPFIETVNNARQNMER
                     INIESDVVCPNCGKKMLVRNSRFGTQFLGCSGYPECKTMLPLTKEGEVHQPEETDEKC
                     EKCSSDMIIKVGPYGKYLQCTNEACNNRKRIVKTTGVKCPKEGCEGEIIQRKSKYGKF
                     FYGCNKYPDCDFVLWNEPTGEVCPKCNSLLIKKVLKKGTTINCSNKACGYSKEVEDGV
                     "
BASE COUNT         3558 a         2035 c         2158 g         4323 t
ORIGIN      
        1 cctacgcttt gttgattcct cgaaaacatc ctgaaagtct gatcataggc atattttaaa
       61 gctacagaag aattattatt aatattgagt cgagaacaga tatccccgta gaaatcatct
      121 aataatttct taaaattaag agcattgttt tgattatctc tatcagaaat tagtacatca
      181 agattcctgt tatatatttc tgcccaagat aaattagaag aaactaatgt atcaagattc
      241 caagaagcag aagttgcttg ttttgtacta ttagcagcat caatgaattc attgcttaat
      301 aattctaata cagacatgct tgtatttgtt gtcgaatcat ttgcaggatt aaagttttct
      361 atataagcat ttgcagtgga tgcatttata ctcattatag cttgtaaaaa ctcagattga
      421 gttttggtga tcgcactgcc tgcaccacta tttgttcctg cggctatgcc cagttttgta
      481 caataatcat cagacaaaat aactctgccc gaagtatctg taagcaagaa ttgctgaccg
      541 agcccgcctg taggagtcat gaatttactg taatttaagg acatggtgtc aacatcaaga
      601 ttgccgtttg cttgccacga agttttataa acagcagaat ccaaagccct gttatgttct
      661 ctggtaattt tttgcgttgc tcttgtcaag gctaaacgtc tcatagtagt gtcttgcatt
      721 cctttttgga tatcgttttg cctacttgtc aaaaaaagta atctgcattg tgatgctgcc
      781 aatcccattg ttttgttcct ttttccctaa ttaaagtatt ttctcgacct ttaaatcctt
      841 tttcctacat catctatcga catatttttt aaataacttt attatttata acaggtttgt
      901 tacaaatttt aacaaatatt tgaaaataca ttaactaccc ctcttgaaaa aaatacaatc
      961 atttgttata atgtgtgagt tatatgtttt ctttttagaa tattcaggtg aagctatgat
     1021 tacacaagtc agtggtcttg attctgttag tgttgcaaaa gatccgcgaa gaaaggctca
     1081 gagcatcaaa tttaagcatc cttgctccaa tatctcttac aaaagcgggg agtacagaga
     1141 tgtttatacg catcaaaaaa atgaggcact tttgacaagt atatcaattg ttgcgggttc
     1201 tactttattt attctgggtt cttttctttt catggcacta aaaaaagtta agccctaaac
     1261 attggttctc ttaatttgtt agtcgaaatt ttaagccgaa tgtatttgtg tgcctaaatt
     1321 ttgtgtcttc atttctttat aggcattaag cccttctttc caattatcca caaattgcat
     1381 gtctttttct gcaacttctt gaggaagtcc tgcgtggtga atttttggta ttaaataatc
     1441 ttccgagtat tcaatttgtt tggggtaatt gtcatcggta tcgttgcata caaaaataag
     1501 ctttccgtct ttgccttgtt ttgtgcctat aatagtaatt tcgtgaccgt ttataataac
     1561 attattttca tctgtttgag tgtatccgat aataacattt tcgcccatgt ttagagtttc
     1621 aagaatgtgt ttttttatgg ttgcaaaatc tgtttcgtat cctgttaatt ttgcattttc
     1681 atccacagtc tgatacgtga ccgaaatttt gtttttgtct tcaacgactg attctgtaaa
     1741 ggttttttca aattcaatta agcctttgtc attttggtta aatttacctg cacgaacatc
     1801 tgtgagcgaa tcgtaagatt gctgtgagcc gacattcata aaagttgatt gcataagcac
     1861 atctaccaaa gaacgttcca gcgggtcttt gtgcgaattt tgaatttgag ctcttgtaat
     1921 tgcactttta tcgggtgcaa gagttaattt tgcagtttta aagtcgttag cttcatacgg
     1981 aacttcaaaa gcgtttaaaa gccaaatagc atcaagcggg gtatttgcaa gtttgtcaag
     2041 atgaattgtt ttttctaccg aaagtttcgg agaagacaag ctttgcgcaa atcttgcaaa
     2101 ttcggcgggc atttgttttg caagattaaa ttcaatactt gccgaaacac atgcccctga
     2161 gtgttctaca tttattgttt gttcgtttat tgggtctttt gtgttttgaa tattcattgc
     2221 tctttgaatt aaatttgtag gaagggtatt atttttgcct atttccagta ttttaggtac
     2281 atattcttta ggtatgtcgc cgaaattttg tgttataaca aaaggatcgg caattgtgtt
     2341 tactgtttct tttaaaacaa cagcagcatc aagcccttgt gctcttggct ctgtgattat
     2401 tttgtaaagg ttatccagtg ttgttgattt gtcgtttgaa tttgaattga gtaatttgcc
     2461 tgtttttaaa agtatttcaa gggtttttct ttcagctttt ggaagcgcgt tacttactgc
     2521 cgaatatttt tctttttctt ctttgtttac aatctctgtt cttatgccgg cggtttcggc
     2581 agcactaaaa gatatagggt ttttagccgt tggaataaca acccctaaag ttttggcggg
     2641 gggctttcct gaaatcacag tcactgctga ttttaaagga tttgtattat aactattgat
     2701 attgtttatt gaatacacaa tttacctcat accgaaaaag gatttttggt tttgacttgt
     2761 gtaaggacac atttaccccc acatatatat tataaaaaca aaaactttta aaatttgcct
     2821 ttttggtata tattttctta agaaaggtta caaaaatttt gaaagaaaaa ataaaaatta
     2881 aaccgcttac aaaatcccag ataattatat ctcttgacag gcttacgagg tttaagccga
     2941 ctgaagaaaa atatttgaga gtttttaaag atattgtcct ttcttgctta attaaaccca
     3001 aatataataa aaaacagctt gatgcaatgc cttatgaaac acttaagggt ttagcggagc
     3061 agatttttaa ttcgtctgtt tcttcacata gtaaaaatcc gcttacgatt aataaaaaac
     3121 tttttgatta tgaagaaaat atattttctt tgaatgaaaa tgtaatgact ttgctggaaa
     3181 ataaaattga ctataattgt gcaattaata tgattgaagg tgaaaaatta ccgcctaatt
     3241 taatctggct caaatcactt tgcaaaaata caaatcaaaa aaaatacaga gcagaatatg
     3301 caataaaatt tcctatagaa aaagttgtta taacagaggg gattacagaa gaaattttgt
     3361 tgccgaattt tgcaaaactg tgcggatatg attttgacaa atacggtgtt tatctgattt
     3421 ctgccggagg aaaaaatcag gttgtaaaac ttttttattc actttgcgag aatttaaaat
     3481 tgcctgtttt tatactttta gacagtgacg caaaatcaaa ttttactcaa atttgtccaa
     3541 aattgagaaa aatcgataaa gtttatatta ttccaaaagg agaatttgaa gatattttgc
     3601 caatttctct tgtcaaaaaa actttgaaca attatcttaa aaatttttct tcgattgatt
     3661 taaaagattt gaagcagaac aaacccatga caaagatttt ggaagaaatt tttaaacaaa
     3721 aaggtttcgg agaatttaaa aaagccgaat tcgcacagat ggtaaaagaa aatatttcgg
     3781 gcaaaattgc tttaagcaaa gatattttaa acatagtcga acagataaag aatatttaat
     3841 aattttgggt aatttggttt aagtaatctt ttactgcact tgtaattatc agcttaggct
     3901 ctcttgagca aaattcgtca agcttgatta taccttgctt tttttgtcct ttttctatgt
     3961 aaataagacc tatcatcaaa gtgttaaagg atgttttggg tttgtttaag taggttttaa
     4021 gctggtaatc aaggcatttt tgggcttgaa aacatttaac aaggttttga tagtaaacaa
     4081 aattcagcgg atatatcttt attgctcttt ggaaacaatc ctgagcccat tttgattgat
     4141 ttatcatcat ataagcttcg ccaagattgt tataataaac cgcacttgcc tgtgaattag
     4201 ggtttaaact tattgcaatt ttatattcct gaatggcggc aaaataattt tgttcgctca
     4261 tgtatgccgc acccagattg ttgtgcatag aagcattttt ttgggcatca attacataaa
     4321 tatcagagcg ttgagctccc gaaaaaatca aggatatcaa caacaaacta aaaattaaaa
     4381 aagatttttt catagtaaat ctatttcaag tccttcgtat gctaaaaatg cgctgtctga
     4441 aattccttca tagtgggaag atatttccgt taatttttca tccgaataag tcgggtcaaa
     4501 atgatagaaa gctaattttt tagcgttcga ttgtttttgg cagtcaagag ccatgtcaaa
     4561 agttgaatgg ccatagcctt gttttggggc gaaaggatct aagtaatctt ccgttgtgta
     4621 ttgtgcatcg tggattaaca aatcgcaatt ccttgcaaac attgaaagct ttttatctcc
     4681 gccaagataa ctttctttgt cggttgcata taccagcgtt ttgtctttgt aagcgatttt
     4741 atagaccata actccttctt gaggatgtgc gtaagattta taacaagaaa ttattatttc
     4801 atcgtttttt ggttttaaat cggctctaaa ggttattttt tcaactttaa tttcatcttt
     4861 atcaattatt atgacttgat tttcatttaa atcaaaaatg tttaaattag ccgcaatgtc
     4921 gcctaaatca agtggaaaag actttgtaaa aagaagttgt gacagttctt cggcaagatt
     4981 ttggctataa tctaccgcac caaagatatt taaggtagtg cttgaaagat gagaaggtct
     5041 gaaaaatgta aaaccttgaa tgtggtcttg atgaatgtgc gataatagca atatggcatt
     5101 aaccggttgt ctgtcatata ttgaatttcc tgacattatg tgttcttgca tcagtaaatt
     5161 gcccaattct attaaccctg ttcccgcatc gagaattatt aatttacctc cgacgttaac
     5221 ttcaacgcag gaagtgtttc ctccgtattt aaggtagtca ttatttgcta ttggatagct
     5281 tcctcttacc cctctgaatt tgactttgaa ttcgtttttt gtgtttctca ttttttcatc
     5341 cttatttttt ttcaataata aaagtggaat tgcgatcttc gtctgctccg taatatttat
     5401 ttgcgccgaa aatcatcatt ttagcacgct tttgaacatc tttatatcgc gtttctttta
     5461 agtaaatatc aaatataact ttgttttttt gtttgtgtat cctcaccagt cgaaaagcat
     5521 tcggataaga cacaagagaa ggggtactga cgtgaagcac attgtctttt tgagttattt
     5581 ttgtagcatg atagtgccct gagaatattg caatagggtt tttatatttt tctataattg
     5641 caagggtctc atcggtattt aatatcctgt ggttagggct gttaagcggt tcgacaagcg
     5701 gcatgtgcaa aaagattaaa acagtgtctt tttttgattt tgaaagctct ttgtcaagcc
     5761 atctaagctg ctcttcgttt atttgtccgt tggctgtgat tctatcatca ataatacagt
     5821 caagggctat aactttatat ccttttttag gtgaaaaact gtaatactgt tttgaaaaaa
     5881 tgaaattctt gttatgggat tttaaaatat ccagataaag ctgttttgaa aggtatccgc
     5941 caattgatat gtcgtgattt ccaaataccg cataccatgg acatttgagc ttgtttgcat
     6001 aaggcaaaaa ttgcattaac tgtttttcgt aagcggtgtt aatcatatcg cctgtgaaca
     6061 ttacaaaatc aacatttggg gtttcgttta cctgatttat cgcatcttcc aaaacctcgc
     6121 ctgattcagc caaaagcctg taagaagtgt tggttttggc ggttgaaaag tgcgcgtcgc
     6181 ttatttgcac aaaagaaaga ttgtcggcag atgccataac ctgcaagccg caaaatatta
     6241 ccgcggcaag aaaaactaca aacaagctgt ttgtcagttt cttaaaaaag ctttcctttt
     6301 ttctttttct gttttttctt ttcatttttc gttaaatctc gtgtttataa cttctttaac
     6361 ttcattataa tggaaattat cgtccaaaac ttcatatatt ttcagttttg aaagataaag
     6421 tgcttttaca tcaggatatt tttttatggc actgttgagc gtatttattg cataactggc
     6481 gtttccgttg tcaaaataaa tatagcttaa atcgatatag tcctgataac ttacagggag
     6541 aatatttgtc gctgaatttg tatatttaat gtaatttttt ttgtcatcca tagatttgta
     6601 gcaaagagca aggttgtaat aataaatcgg tcttagcccg tcaagctcta ttgctttggt
     6661 gaaatcagac atagcttcat ttatgttttt tagtttatag ttgcaaagtc ccaaataatt
     6721 aagtacttcg gcagaatttt tggtcttgaa aacttcttga aaaatattta gtgcttctat
     6781 gtaattttca aggtagtatt cgatttttgc aagttcgtat ttgtattgaa ttgaataact
     6841 tatttccatg gcttttagta catcttgttt tgcggcttcg taacggccta aaataagatt
     6901 tgttcttgca gacatgtaat aagagtagtc cgaattgggg ttaagctcgt tatatttatt
     6961 gagtgcggca agagtatagt tgaattcttc ggtttcaaaa aatgtcattg caagagccat
     7021 gtacgcctgg gtaaatttgt cgttaatttt taccgcttct ttgtagtttt caagggcttt
     7081 agggtacatt cctttgtctt tataataatc gcctaaaaga taatatgccc agtagtcgtt
     7141 tggattgcat tttgttgctt tttgaaggca gtaaaactct tgtttgacac aacccagttg
     7201 tttataaagt aacgccatat ttacaagcgg ctgagtgtaa tttttgttta gccaatgagc
     7261 ttttttaaaa tatttgattg ccaaagtcgg attgttttta agccagtaac acatcccgag
     7321 gttgttgtag gcttgagcat ttcttttatc cagatttatt gctttaaaaa agtattctat
     7381 ggcaacattt aaatcttttt tggcataaaa ttcctgacct ttttggttgt aataatcgga
     7441 atcataatcc attttttcaa catcggcaga agtgacattt aaaacctcaa atctttttag
     7501 cggttctatg tcatcaggca ctccgacaag ctgttgttga actttagcag gttcttccgt
     7561 caatggtgct tgaggttctt cttgtgctgt aactgtttgt gcgccggtta aaggcagaat
     7621 tttatttttt aaatcaatcg cacattggtt ttgaggctct atttttaaga tttcatcaac
     7681 atattcaagt gccttttgat aattatttgt gttgaaatag tttgtagcaa tgtctaaata
     7741 gtcatccgta atttcatttc caagtgcaaa acacggattt aatatgaaaa gacacaaccc
     7801 tattaaaatt aaaaaaagtc tttgcttcat aagccaatta tagcatagat ttttttttgt
     7861 agaagagggt atttttaaaa ttatttaatc aacaaaagac ttatccaaat taagaaaata
     7921 cccgacataa tcccgaaaac cgaatagtga ccgtcgccgt attctcttga taaaggcaaa
     7981 agggtgtcaa aggatatata aatcataatt cctgcaactg ctgcaaacaa aaatccgatt
     8041 gcaatttgag gaagaaaata atgcatcaca agtaaaccta taatagcacc tatcggttct
     8101 gaaattccgg aaagaaaaga ataccatatc gccatacgtt ttttgcctgt tgcatgataa
     8161 atgggaagcg caacggcaat tccttcggga atgttgtgaa gcgcaatcgc tattgcaaca
     8221 gagatgccca aggtaacatt ttgggttgta acaaaaaatg ttgccattcc ttccgggaag
     8281 ttatgtattg caatggctat tgccgttaaa attcccgctc ttctgatttt atggtgtttg
     8341 taagagcata taatatcatt ttcgtcatca ttttcttttc tgtggcggtg ttttctgttt
     8401 ttgcagtatt ccggcggacc aaaaaggttt tcatcaatgt ggtccggaat aaaatagtct
     8461 atcaaaatag caatcaccat tccgaaagta aatccgaaaa aggtcatcca atttgcgtta
     8521 tttggataaa aaggttgtaa aagtttttgt gcgccttgca gaatttcggt gaaagataaa
     8581 aaaatcatta cccctgcaga aaatccaagc cctatagaaa gggcttttat gtttgtcctt
     8641 tttatgaaaa aggtaaacat tccgccaact actgttgcaa gccccgcaag gaaggtcatt
     8701 gttattgcta tttcataatt tgcaggtgtg tgtggcattt ttcttctcgt ttcttaatta
     8761 attttactac aagcgtgaga aaaatgaaag ggcgaataaa ccttgatccc ctcaccccag
     8821 ccctcatcct acggtacccc tcaccccagc cctctcccgc aaggggagag ggagaaaaat
     8881 tatttttgca atccttagcg caaggactgt gccgaacaaa acgattaagt atcgttttgt
     8941 gagaggtaag ggagaataat tacttcttaa atacttagga aacttttttg ctaaaaaaag
     9001 gtctaatatt atgttgggga attttatgga agtattttat catcttgttt atgaatatca
     9061 aaaagggctt agggatttat gtctttttac ttgtgttgac aaattttttg ataaaataaa
     9121 aaaaatactt gaataccaaa aaattgatta tttggtattg gcattacctg aggacaaaat
     9181 taatatcttt ttcgggctga aagactgcat tgatatcgtc aaaagttttt cgtcagaaga
     9241 gttgaattta cttgcgcccg aagaagattt tattttagga atgatgctcg gttattcaag
     9301 gcaaaaacag tacagcagat ttttaaaaaa gaaaaacctg attgaattta atcaggttgt
     9361 agctgaatcg tgttaagtat aaagttaaac ataaaattat ttaaccctgt ttcttttata
     9421 ctttataatc gaaaataaat atactagtca tctgggtata aaattaattt ctgatagaaa
     9481 ttttgttttt caataactct tgtttcagtg tttttatagg caaagtgtta tagtgaaata
     9541 ttgaagcagc aagagcagca tctgcatgtg ctgtattaaa cacatcaaca aaatgtgaaa
     9601 taaaagcacc cgcaccgcct gatgctataa caggaatatt cagagcatct gcaattgttt
     9661 gtgtcatttt taaatcaaat cctctttgtg ttccgtcagc atccatcgac gtcaaaagaa
     9721 tttctcccgc acccagttct tctgcttttt ttgcccaatt taaaagtttt aatccggtgt
     9781 tttttttgcc ggcgtttata aaaacgtaaa aatctttgtc aataagtttt gcgtcaatgg
     9841 ctacaacaac acactgcgag ccgaagtatt gcgaagcttc ttttattaat tcaggatttt
     9901 tgactgccgc ggagtttatc gcaactttgt cggcacctgc tgaaagcagg tgcctcatat
     9961 cttcaatcga attaattccg ccacctaccg taaaaggtat aaaaacctgt tttgcgactt
    10021 ttttaaccat ttcaacggta gttttgcgcc cttcgtttgt ggctgttata tcgagaaaaa
    10081 caagctcgtc tgcgccttca tcagagtatt tttttgcaag cgcaacaggg tctcccgcat
    10141 attgcaggtt ttcaaagtta acccctttaa ctgtttgccc gtctttaaca tcaagacagg
    10201 gaataattct ttttgcaagc atagtttata ccaataattc ttccaatgcc gctattttca
    10261 ttttatcggt gtcgggcatg aagcctgtcc agagttccaa agattttgca gcttggtgaa
    10321 tcagcatatc aagcccgttt attgtctgaa tcccgcgctg agaaactttt tcaagaaaaa
    10381 gcgtttttac cggattgtaa attacatcaa aaacaattgt atctgtggga agttcgtgaa
    10441 ttatttgcat acttaaaggc attttatctg ccgaatgacc tttcatacct gtgggtgttg
    10501 catttactat catttttgat tcaagaagtt ccataaaact ttgcatttga taaagtttaa
    10561 aagtagtctg cgaaaatgct tttcgcatgt agtttaccat tgtcgaagaa tttataatat
    10621 ttcttgaaaa gaaacttatt tgcttaaccc ccagttcaat taaacccaca gcgcatgccc
    10681 ttgcagctcc gcctgttccg atgatgctca catttgcgtc tttgagttta ggttttacgt
    10741 cttctgggat tgcgttttta aagccgtaaa cgtcggtgtt gaagcctgac aaagatttat
    10801 cgggaagaat tttaaccgta tttacgcatc ctgcaacatc ggcatattga tcaacttggt
    10861 ctaaaaataa agaaacagga acttttagcg gaattgtaac gttaaatccg ttaaaatcat
    10921 ttgctttgag aaattttaag cgtgaaatta attcatcggg cggcgtttca agaatttcgt
    10981 aagaaccttc gatgcctaaa gtcttaaaac ctgctttttg gattaccgca gaaacggaat
    11041 gtgtgagcgg agagcctatt aatgcaaatt tatacgccat cttcaacctc ctttgaataa
    11101 ccgcaggctt tatttgagca gtttatggtc gtaccttttt tcaaaacttt tttgatcaaa
    11161 agtgaattgc atttggggca aacttcgcct gtgggctcat tccagagcac aaagtcgcaa
    11221 tcgggatatt tgttgcagcc gtagaaaaat tttccgtatt ttgactttct ttggataatt
    11281 tcaccttcgc agccttcttt ggggcatttt acgcctgttg ttttaactat ccgttttctg
    11341 ttattgcagg cttcgtttgt gcattgcaga tacttcccgt aaggtccgac ttttataatc
    11401 atatcggagg aacatttttc gcatttttca tctgtttctt cgggctgatg aacttcgcct
    11461 tctttggtca aaggaagcat tgttttacac tcgggatatc cggagcagcc taaaaattgg
    11521 gttccgaatc ggctgtttct gacaagcatt tttttgccgc agttagggca gactacgtcg
    11581 ctttcaatat ttattcgctc catgttttgt cttgcgttgt ttactgtttc tataaaagga
    11641 gtgtaaaatt ctttcaagac ttcattccaa ataagttttt tttctgcaac atcatcaagt
    11701 ttattttcca ttcccgcagt aaatttgtaa tccataattt ttgtaaaatg ctccacaagc
    11761 tgttttgaaa cggttttacc caaaatggtc ggagcaagtg ccttttctat tttttccaca
    11821 taacctttag tctgtatttt ggtgataata ggcgcataag tggaaggtct tccgatgccg
    11881 tgttcttcaa gtgcttttac cagagaagct tcgctgaatc ttggcggagg ctgggtaaaa
    11941 tgttgttttg gattgacttt tttgcatttt aaaacttcgc cttttgtgaa ttcaggcatt
    12001 gagtttttgc agatttcttc ctcatcctcg cagtaaattt tcaaaaaccc gtcgaaaatt
    12061 attttgcttg aacc
//
LOCUS       DAOQAU010000051        11882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_6084_length_11882_cov_6.012006, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000051 DAOQAU010000000
VERSION     DAOQAU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6084_length_11882_cov_6.012006"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PKI94_07165"
     CDS             <1..848
                     /locus_tag="PKI94_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW26549.1"
                     /translation="YLRIFMKEVFTQLRHAELVSASNPYTHVGKILRQAQDDRKKGFT
                     LAEVLITLTIIGVIAALTIPNLIASYQKQQYVTQLKKVYTEISQAMKQVMADEGINKL
                     TLSDTLYCDYENGCDLDEALQRVGNDFLKKYFKVIKDCGTEYPSPCFASKYNTINGNE
                     GSVIPSGYSVINVGGASISIFPTQKQYSGTIYVDINGLNPPNIYGRDLFTMAFFYDGS
                     IDEFGINPQCRKGEISVNGLGWLCNGKGNAYEARESMFEEYCDHQGYGSCFGKILNDN
                     WKMDY"
     gene            complement(851..1534)
                     /locus_tag="PKI94_07170"
     CDS             complement(851..1534)
                     /locus_tag="PKI94_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNW26550.1"
                     /translation="METAKIKEIFASIQGEGPYVGVKQLFIRFSGCNLNCNFCDTDEE
                     DCRGKFPDYTPEQLAKHIEQFDLKNIHTVSLTGGEPLLWADFLKEFLPLINNKIYLET
                     NSTLNTQIPKIIDMVDIISADIKLPSASGVEKSFALHDEFFKVLQGYNKEVFAKIVFD
                     ENILDDEIAHCIKLAEKYKLPLILQPKTQDYELSVSGEKIIEVFDKFLTKYPNVRVIP
                     QVHKYLQVL"
     gene            complement(1521..2558)
                     /locus_tag="PKI94_07175"
     CDS             complement(1521..2558)
                     /locus_tag="PKI94_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNW26551.1"
                     /translation="MVSSEKNLYDILEISADASPVAIKSAYRKLARKYHPDVNSGNSA
                     CIKKFKEITEAYEILSDEEKKKNYDLLRGFYKHEETQAKRKQANKAYKESQNFEEETK
                     KEEPEFSNIFNDILEGFKKTTGKKNFEQKQAYPEKGSDVFTDISITFSEALKGTTRKI
                     NILHTQKCPNCEGRKFINGTKCPVCGGLGEQSVYKKLSVKIPPNVKNGSKIRIANEGN
                     KGYNGGKNGDLYLKIKIENSDIFKFEGLNILCAIPITPSEAVLGASINIPTIEGNVLM
                     KIMPNTHSGQKFRLSGQGLKGNDGKYGDMIVTVNIEIPKDLSQKEKELYEELKNLSKK
                     DIRENLFNGNS"
     gene            complement(2666..3229)
                     /locus_tag="PKI94_07180"
     CDS             complement(2666..3229)
                     /locus_tag="PKI94_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNW26552.1"
                     /translation="MNYIDIFILAIALSIDACVVSFSNGLIFAHNKVKNSLILAFSVG
                     FFQFLMPLIGASFTGLVSKYIEPYSAWVVFAIFMILGLKFIKDAYKQKEQEKLLCYLC
                     LSYILAISLATSIDALAAGVSLALFGVRILIPAIIIGVITFINSLLGFWAGNFFKKFP
                     TKNLEISGGVILILLAFKILLENFKII"
     gene            complement(3226..4695)
                     /locus_tag="PKI94_07185"
     CDS             complement(3226..4695)
                     /locus_tag="PKI94_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HNW26553.1"
                     /translation="MKKIALIPIDNRPVCYTLPEQIAKIDENIKLFMPDRKFLGGLNS
                     QAKVKDIFKWLKNLENVDAIIISLDTIAYGGLISSRRCPQTFDEIKSNLEKFKKLLEQ
                     KNTKIYAFSSIMRISNNNINEEEKEYWNKYGKKIFKYSRKFHEEGIETKDVPKEILSD
                     YLATRQRNFEINKIYLEWIEEGVFDTLVFSKDDCAPFGLNVLEAQILKKMIEEKKLNA
                     LVKIGADEIPLTLLARAVVELNRMPKISVKFLAPEHKDLISNYEDISIKESVEEQLKL
                     AGCKIVKKEQSEIELIVNNFEQKQGEIVMEIETIPFSGNIHLPPRPYMIADVRFANGA
                     DNAFVEKLFKAGLNFENFYGYSAWNTSANTLGSLICAAIVRFFAKKYNENEFKKLQFV
                     RFADDWAYQANVRQSLKNTPHPNPITLQYPSPQPSPARGEGEQLFLQSLAQEAKIKSK
                     LNEMMSDFENRINKKLNTNFSAKYSYPWDRFFEVDVMLK"
     gene            4755..5393
                     /locus_tag="PKI94_07190"
     CDS             4755..5393
                     /locus_tag="PKI94_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26554.1"
                     /translation="MKKILVLILILFFAPCAFGFNFFKQKQKFPAEGGYVGTLPNVAE
                     KFQKPQIQEAAPVFDRVESFRDQSQIKPLPRDNPAFVNIILKKDKTSQYLNDINDVIL
                     ALEKLANIIETKQDIQRFNAEAYYVKANVEYLRNKYKNKSETSYVSYQKLMKLNLQIQ
                     TIAQLRSDGSKYSQYLASSDGGYVYNENNIDKQMDYLLQEINDTLFVLRDVR"
     gene            5546..6472
                     /locus_tag="PKI94_07195"
     CDS             5546..6472
                     /locus_tag="PKI94_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26555.1"
                     /translation="MLGVTFYLFINNFVFGRIIMGQANNDDLLKKYNSAITLYNVADF
                     YYTLNHFSDENKQIYFQIPYKKAKCYMENKKKKESIQSMLQGVTKIQRQYGIFSRETA
                     CFMRKYLIEYYLDNNSYTLANQEFNNLLTIYKNIGYDNNEMADMVRLSGDLSFEAADY
                     ESAMEFYQRAYDIISRQPDIDDEVFMKITSRIALYEVQQDKIDLAINIYSNSIETLKN
                     SENPQKSVIADMLIQLGDIYANAGKPKNAIICYEEAVELIKKLPRVNFMRQNLNFYLN
                     SLKDLYDKAGQFHKVREIELEFARQRRFFFML"
     gene            6603..7247
                     /locus_tag="PKI94_07200"
     CDS             6603..7247
                     /locus_tag="PKI94_07200"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNW26556.1"
                     /translation="MFSPTIQNLISTGGQKAAEQRAVQIQNYVNNLTAPQTIAPPNIK
                     TKSFQEVLQTSNPANFGALLSNPESMSVQAQIVNAPALRDYTKPSKPQLLSMISQIAK
                     KHGVDEKLVHAVVKQESGFNPKATSHCGAMGLMQLMPATAKGLGVTDPYNPVQNVEGG
                     VRYLKNLLNKYNGNIILSLAAYNAGSGAVDKYDGVPPYKETQNYVKSILANYLS"
     gene            7522..8436
                     /locus_tag="PKI94_07205"
     CDS             7522..8436
                     /locus_tag="PKI94_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNW26557.1"
                     /translation="MKNSKITVKKKNNFTNPSRNSVLKSDSENLLGIYLKEIASCPFL
                     SSSEEKIVAKKAKEGDLEAKKLLVQANLKLVVTIARKAIHIAKIPMIDLIQEGNLGLM
                     IAAEKFNWKLGYKFSTYASWWIKQSMFKAISEQAHCMKIPVYIQETLSKFSKIKSQME
                     NKYNCQVKTSEVAQKMNIPPDKIESYLSAYSKTVSLESGFENENGKEFCMADILEDEK
                     ANVFETVEYEGLKKDIEFVVSTLKEREQEVVRMRYGLGNFAKKTLEEIGNLYGVTKEC
                     IRQTELRALKKMKGSAFGSELLSCYIAG"
     gene            8589..8888
                     /locus_tag="PKI94_07210"
     CDS             8589..8888
                     /locus_tag="PKI94_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HNW26558.1"
                     /translation="MQDQTYQNEQLQCVECGKIFEFSAEDQEFYAQKGYSSPKRCPEC
                     RENRKARSNNSRGGYRSERRDYNVTCAACGCQTTVPFKPSNDRPVFCSDCYRKGQ"
     gene            complement(9085..9837)
                     /locus_tag="PKI94_07215"
     CDS             complement(9085..9837)
                     /locus_tag="PKI94_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26559.1"
                     /translation="MTKINFTQLPTNFNFGENQNYHGSFAFIKKQAHEKHIDEFFRTN
                     FNAPKKKNSLIAFFTSIAGVAIPLLLIGKRQKPEIKMNSLKNILKITDIHYDFGSIFA
                     LGTGGVVGGLLGGLADKNERKKSRKLKEGAYQLMNVAIPALFVSAGMKFCESIPKLNK
                     SLPKIAATGIGIWTGVNLAVWLANKFENKVFDPHEISPDRKFKNKDLIVHVDDLIGAL
                     VLAKMPFMEKLKIGRALPFIFAWCGYHVGDKQ"
     gene            10088..10363
                     /locus_tag="PKI94_07220"
     CDS             10088..10363
                     /locus_tag="PKI94_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26560.1"
                     /translation="MITPVISRLSAINSMNNAAYGMMQSSNALMGLISFAGTNPNVDL
                     GKLNSVEKNLMMKMLNDSFIYKISQAQEKQLKKLEQDNIKHSFSTFA"
     gene            complement(10387..11463)
                     /locus_tag="PKI94_07225"
     CDS             complement(10387..11463)
                     /locus_tag="PKI94_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNW26561.1"
                     /translation="MEIAIVLLILVLIIAFVFEYINGFHDSANAISTVISTKALSPRH
                     AIIYAATLDFTGAFFGTHVAHTIGAGLVEGACITQLVIFCALAGAVLWNLFTWWLGLP
                     SSSSHALIGGLLGASISHAYFELDRHYELILTLPSLRLHHPALNVINIQNVINKVLLP
                     MVISPVLGFTIGFLVILVLLSIFHKMRPEKLNKLFRKLQLFSSGFLAFSHGSNDAQKT
                     MGIITLALMTQGIIHNKSFEIPVWVICACAMTLSLGTMTGGWKIIRTMSSKVIKMKPI
                     HGFSVEMASASIIITASHFGFPVSTTHIISTAIVGVGSMVKASAVKWGIVKKIVAAWI
                     FTIPICMLLSGIIYFTLMKTPIAD"
     gene            complement(11469..>11882)
                     /locus_tag="PKI94_07230"
     CDS             complement(11469..>11882)
                     /locus_tag="PKI94_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNW26562.1"
                     /translation="PIDREDIQALAIMLNKITKKTAQAFMNLNVYRIENFTEEMLQQA
                     KTLVNATEELKRSVGLLKTISKTKEITESREAMKEIETYGDNILYRAMDNLFSGKFEA
                     IEVIKLRDIYKDLERAMDKCFSVSDAVLTIALKNN"
BASE COUNT         3835 a         1948 c         2118 g         3981 t
ORIGIN      
        1 aatatttaag gatttttatg aaagaagtat ttacacaatt acgtcatgct gaacttgttt
       61 cagcatctaa cccgtatacg catgttggaa agatcctgag acaagctcag gatgacagga
      121 aaaaaggatt tacgcttgcg gaggttttga taactcttac tataataggt gtaattgctg
      181 ctttgactat accaaatttg attgccagtt atcaaaaaca gcagtatgtg acgcaattaa
      241 aaaaggttta tacggaaatt tcacaagcca tgaaacaagt aatggcagat gaagggatta
      301 ataaacttac cttgtcggat actttgtatt gcgattatga aaacggatgt gatctggatg
      361 aagctttgca gcgtgtggga aatgattttt taaaaaaata ttttaaagtt ataaaagatt
      421 gcgggacaga atatccgagt ccctgttttg catccaaata taatacaatt aatggaaacg
      481 aaggatctgt tataccttct ggttatagtg taataaatgt aggtggggct agcatttcta
      541 tatttccgac acaaaaacaa tattcaggaa ccatctatgt ggatataaat ggtttaaacc
      601 ctcctaatat ttacggaagg gatcttttta cgatggcttt tttctatgac ggttcaattg
      661 atgaatttgg aataaatccc caatgtagaa aaggggaaat atcagttaac ggtttgggat
      721 ggctttgcaa tggaaaaggt aacgcttatg aagctcgtga gagtatgttt gaagaatatt
      781 gcgatcacca aggctacggc tcatgttttg gtaaaatctt aaacgataat tggaaaatgg
      841 attattaaaa ttataaaacc tgtaaatatt tgtggacctg cggaataact cttacattgg
      901 gatattttgt caaaaattta tcaaaaactt caataatttt ttctcccgaa acgctcaatt
      961 cgtaatcttg cgttttgggc tgtaaaatca gcggcagttt atatttttcc gcaagcttga
     1021 tgcagtgggc aatttcatcg tctaaaatat tttcatcaaa aacaattttt gcaaaaactt
     1081 ctttgttata cccttgcaaa actttgaaaa actcatcgtg aagcgcaaag gatttttcta
     1141 caccggaggc acttggaagc ttaatatcgg ctgaaattat gtcaaccata tcaatgattt
     1201 ttggaatttg agtgtttagg gtagaatttg tttcgaggta aattttatta ttaattaaag
     1261 gcaaaaattc ttttaaaaaa tccgcccaaa gcaacggctc gccccctgtc aaagaaacgg
     1321 tgtggatgtt ttttaagtca aattgttcta tgtgcttggc aagctgctcg ggtgtgtagt
     1381 cagggaattt gcctcggcag tcttcttcat cagtatcaca aaaattacag tttaagttgc
     1441 agccgctaaa tcttataaaa agctgtttta cgcctacata agggccttct ccctgaattg
     1501 atgcaaatat ttcttttatt ttagctgttt ccattgaata aattctctct gatgtctttt
     1561 tttgaaagat tttttaattc ttcataaagc tctttttctt tttgtgaaag gtctttggga
     1621 atttcaatat tgactgtgac aatcatatct ccgtattttc cgtcatttcc tttcaagccc
     1681 tgccccgata gtctgaattt ttgccctgaa tgtgtatttg gcatgatttt catcaaaaca
     1741 ttgccttcta tggtcggaat atttattgac gcgcctaaaa cggcttcact cggtgttata
     1801 gggattgcac atagaatatt caaaccttca aacttaaaaa tgtcgctgtt ttcaattttt
     1861 atttttaaat aaagatcacc attcttgcct ccattgtaac ctttattgcc ttcgtttgca
     1921 atccttattt tagagccgtt tttgacattt ggcggaattt ttacgctgag ttttttgtaa
     1981 acggattgtt cgcccaaccc gccgcacacg ggacattttg ttccgttaat aaattttctg
     2041 ccctcgcagt tggggcattt ttgggtgtga agaatgttta tttttcttgt ggttcctttt
     2101 aaggcttcgg aaaatgttat tgaaatatcg gtaaaaacgt cgcttccttt ttcagggtaa
     2161 gcttgttttt gttcaaaatt tttcttcccc gtcgtttttt taaaaccttc taagatgtcg
     2221 ttgaaaatat ttgaaaattc cggttcttct tttttggttt cttcttcaaa attttggctt
     2281 tctttatatg ccttatttgc ctgctttctc ttggcttgcg tttcttcgtg tttgtaaaat
     2341 cctctcaaca ggtcataatt ttttttcttt tcttcatctg ataaaatttc gtaagcctct
     2401 gttatttctt tgaatttttt tatgcatgcc gaattccctg aatttacgtc aggatgatat
     2461 tttctggcaa gttttctgta agctgatttt atagccacgg ggcttgcatc tgctgaaatt
     2521 tccaaaatat cgtacaaatt tttttctgat gaaaccacca aaattcacct ctttgattct
     2581 taagcacaat gagcttcatg catcttttgt aattatttaa tgaaaatttt aaaaatttca
     2641 atagtccttc aatattaatt tttggctata tgattttaaa gttctcaagc agaattttga
     2701 aagcaagtag aattaaaatt accccgcctg agatttccaa gttttttgta gggaattttt
     2761 tgaaaaaatt ccctgcccaa aatcccaaaa gtgaattaat aaaggttatt acccctatta
     2821 taattgcagg gataaggatt cttactccga aaagagccaa tgaaacccct gcggcaagag
     2881 cgtcaatgct tgtggcaagc gatattgcta aaatatagct taagcaaaga taacacaaaa
     2941 gtttttcctg ttctttttgt ttgtaagcat cttttatgaa tttaagtcct aatatcataa
     3001 aaattgcaaa aacaacccat gccgaatacg gttcaatata tttacttacc agccctgtga
     3061 agcttgcgcc gattaacggc atcaaaaatt gaaaaaagcc tacagaaaaa gccaaaatca
     3121 aagaattttt tactttgttg tgagcaaaaa tcagtccatt tgaaaacgaa accacacagg
     3181 cgtcaataga aagtgctatc gcaagaataa agatgtctat ataattcatt tcagcatcac
     3241 gtccacttca aaaaacctat cccagggata agaatacttt gcactaaagt ttgtatttaa
     3301 ttttttattt attctatttt caaaatccga catcatttca tttaattttg attttatctt
     3361 cgcctcttgc gctaaggatt gcaaaaataa ttgttctccc tctccccttg cgggagaggg
     3421 ttggggtgag gggtattgta aggtaatagg gttggggtga ggagtatttt ttaaggattg
     3481 ccttacattt gcctggtatg cccaatcatc ggcaaatctt acaaattgaa gttttttgaa
     3541 ctcattttca ttatattttt ttgcaaaaaa tctcacaata gcagcgcata ttaaacttcc
     3601 taaagtattt gcactcgtgt tccatgcgct gtatccgtaa aaattttcaa aatttaatcc
     3661 cgctttaaat aatttttcca caaaagcatt gtcagctccg tttgcaaagc ggacatctgc
     3721 tatcatataa ggccttgggg gtaaatgtat attcccgctg aagggtattg tttcaatttc
     3781 cataacaatt tcgccttgtt tttgttcaaa attgttcaca ataagttcaa tttctgactg
     3841 ttcttttttg acaattttac agcctgcaag ctttaactgt tcttcaacgc tttctttaat
     3901 tgaaatgtct tcgtagtttg atattaaatc tttgtgttca ggtgccaaaa atttcacaga
     3961 aatttttggc attctgttaa gctcaacaac cgcacgtgcc aaaagcgtca atgggatttc
     4021 atctgcgccg atttttacaa gtgcgtttaa ttttttttct tcaatcattt tttttaggat
     4081 ttgggcttcc agaacattga gcccgaaagg cgcacaatcg tcttttgaaa aaacaagagt
     4141 gtcgaaaaca ccttcttcaa tccactcaag gtatattttg ttaatttcaa aatttctctg
     4201 tcttgtcgca aggtaatcac ttagaatttc ttttgggaca tcttttgttt caattccttc
     4261 ttcgtgaaat ttccttgaat atttaaatat ttttttgccg tatttattcc agtattcttt
     4321 ttcttcttcg tttatgttgt tgtttgaaat tctcataata cttgaaaaag catatatttt
     4381 tgtgtttttt tgttcaagaa gctttttaaa tttttcaaga tttgatttaa tttcatcaaa
     4441 tgtttgaggg catcttctgg aagaaattaa accgccgtat gctatggtat caagcgaaat
     4501 tattattgca tcaacatttt caaggttttt aagccactta aaaatgtctt taacttttgc
     4561 ttgagaatta agtccgccta aaaactttct gtcgggcata aaaagcttga tattttcgtc
     4621 aatctttgca atctgctcgg gcagagtata gcagacaggg cgattgtcaa taggaatcaa
     4681 tgcgattttt ttcataactg agttattata tcataacggt tgacctttgg gcaaaaaaca
     4741 gtataatttt aagtatgaag aagattttgg tgcttatttt aatactgttt tttgccccct
     4801 gcgcttttgg gttcaatttt tttaaacaaa aacaaaaatt tcctgcagaa ggcggttatg
     4861 tagggacttt gcccaatgtt gccgaaaaat ttcaaaagcc tcaaatacaa gaagcagcac
     4921 ctgtttttga cagggtagaa agttttcgtg accaaagtca gataaagcct ttaccaagag
     4981 ataatcctgc atttgtaaat ataattttaa aaaaagacaa aacctctcaa tatttaaatg
     5041 atataaatga tgttatttta gcattggaaa agcttgccaa tattattgaa acaaagcagg
     5101 atatccaaag gtttaatgct gaggcttact atgttaaagc aaatgttgaa tacttaagga
     5161 ataaatacaa aaataaatcc gagacaagct atgtttctta tcaaaaactt atgaagctta
     5221 atttgcaaat acaaactatt gctcagttaa ggtcggatgg ttctaagtat tcacaatatc
     5281 ttgccagcag tgacggtgga tatgtttata acgaaaataa tatagataaa caaatggatt
     5341 atttgcttca agaaataaac gacactttgt ttgtattaag agatgtaagg tgagagtgtt
     5401 gacagagagg ctttataaag gttaatattg gttaaataag tttgtgtaga aaaaggtttg
     5461 aatgggtctg agaaaaattt atttaagaat aagaatattt ttttactcta tcagaagatt
     5521 aactaatact ctgatgatct cattaatgct gggcgtaacc ttctatcttt ttataaataa
     5581 ctttgtattt ggcagaataa taatgggaca ggcaaataac gacgaccttt tgaaaaaata
     5641 taattctgct ataactttat acaatgttgc tgatttttac tacactttaa atcatttttc
     5701 cgatgaaaac aagcaaattt attttcaaat tccttataaa aaagccaagt gctatatgga
     5761 gaataaaaag aaaaaagaat ctattcaatc tatgctgcag ggtgttacca aaattcaaag
     5821 acagtacggc attttttcaa gagagacagc ttgttttatg agaaaatatc ttattgaata
     5881 ttaccttgat aataacagtt atactcttgc aaatcaggag tttaacaatt tgctcacaat
     5941 atacaaaaat ataggatatg ataataatga aatggctgat atggtaaggc tttcgggtga
     6001 tttgtcattt gaggcggccg attatgaaag tgcgatggaa ttttatcaaa gggcctatga
     6061 tataataagc cgacagcccg atattgatga tgaagtattc atgaaaataa caagcagaat
     6121 tgctttgtat gaagtacagc aagacaaaat agatcttgct atcaacatat acagcaattc
     6181 tattgaaaca ctaaaaaaca gcgaaaatcc tcagaaaagc gtgattgctg acatgttgat
     6241 tcagctggga gatatatatg caaatgcagg taaacctaaa aatgctataa tctgctatga
     6301 agaagcggtt gaattgatta aaaaattgcc gcgggttaat tttatgcgtc aaaaccttaa
     6361 cttttacctg aattctctta aagatttata tgataaggcc ggacaattcc ataaggttag
     6421 agaaatagag cttgaatttg caaggcaaag aagattcttt ttcatgcttt agttgacttt
     6481 tttgtcatcg gagttaaaaa aataccagcc atatagtgga ttttttaaag aaatcctaaa
     6541 actttggtgt gattattacg acaaaaacaa tgtgggcggt ttaaattaaa aagggcgaaa
     6601 aaatgttttc accgacaata caaaatttaa tatcaacggg agggcaaaaa gctgccgaac
     6661 aaagagctgt gcaaatccag aattatgtaa ataatcttac cgcaccccaa accattgcgc
     6721 cgcctaatat caaaacaaaa tcttttcagg aagttttaca aaccagtaac cctgctaatt
     6781 ttggggcact tttgtctaat cccgaatcaa tgagcgttca ggctcaaata gtaaatgctc
     6841 ctgctttgcg cgattacaca aagccttcta agcctcagct tttgagtatg atatctcaaa
     6901 tagcaaaaaa acacggagta gatgaaaaat tagtgcatgc cgtggtgaag caagaatcag
     6961 ggtttaatcc taaagcaact tcccattgcg gagcaatggg attaatgcag ttaatgcctg
     7021 caacggcaaa aggtcttggg gttacagacc cttacaaccc cgtgcaaaac gtagaaggcg
     7081 gagtaagata cttaaaaaat cttctcaata aatataatgg taatataatt ttatctcttg
     7141 ctgcttataa cgcaggatca ggtgctgttg acaagtatga cggggtaccg ccttataaag
     7201 aaacacaaaa ttacgttaaa agcattcttg caaattattt gagctgattt ttgaaataga
     7261 tttttattaa gatatattac aaatattttt aaaaaaacaa actcgtttta acatttctta
     7321 acaaaccact tgtatttttt tgctcaatat gatatattga gtatatactc tttcaatcaa
     7381 gttttatacc tgttgttgtg cttatagttt aactaaacgc ctattattgc ttgtttgcgt
     7441 tgatttttaa tgcgcgaaaa agtaagtttt ggatggcgtg aattgtgttg aaaaatttac
     7501 ttataaaata ggagtccagt tatgaaaaat tcaaaaatta ctgtgaagaa aaaaaacaat
     7561 tttacaaatc cttcaagaaa ttcagttctc aaaagtgatt ctgaaaacct tttgggcatt
     7621 tatttgaagg aaattgcaag ttgtcctttt ttgtcttctt ctgaggaaaa aatcgttgct
     7681 aaaaaagcaa aagaaggtga tttagaagca aaaaaacttt tggttcaggc aaatttaaaa
     7741 ttagttgtta caattgccag aaaagcaata catattgcta aaattcctat gattgatttg
     7801 attcaggaag gaaatttggg attgatgatt gcggcagaaa aatttaactg gaaactaggc
     7861 tataaatttt ccacttatgc aagctggtgg attaaacagt caatgtttaa agctatttca
     7921 gagcaggctc attgcatgaa aattcctgtt tatattcaag aaactttgtc gaaattttct
     7981 aaaatcaaat ctcaaatgga aaataaatat aactgtcagg tcaaaacctc agaggttgct
     8041 caaaaaatga atattcctcc tgataaaatc gaaagttact tatccgctta ttcaaaaaca
     8101 gtttctttag aaagcggttt tgaaaacgaa aacggtaagg aattttgcat ggcagatatt
     8161 cttgaagatg aaaaagcaaa tgtctttgaa acggttgaat atgaagggct taaaaaagat
     8221 attgaatttg ttgtttctac ccttaaagag cgtgaacaag aggtcgttcg tatgcgctac
     8281 gggcttggta attttgccaa aaagacatta gaagaaatag ggaatctcta cggcgtgaca
     8341 aaagaatgta tcaggcaaac agaactcaga gcacttaaaa aaatgaaggg aagtgctttt
     8401 ggcagtgaat tgttgtcctg ttatatcgcc ggataattaa atttttcctt gtgtttaaat
     8461 gggtagaaaa attataaaaa tcgtataatt ttttacccat tttttatgtt aaaaaaaatt
     8521 tactggattt ttattatttt tgtatcataa tgaagatgtt acacaaaata aaaagaaaga
     8581 aaaaaacaat gcaagatcaa acttatcaaa atgaacagtt acagtgtgtt gaatgcggga
     8641 aaattttcga attttcagct gaagaccaag agttttatgc tcaaaaaggg tattcttcac
     8701 caaaaagatg tcctgaatgt cgcgaaaaca gaaaagcaag aagcaacaat tcaagaggcg
     8761 gatatcgttc agaaagacgc gactacaatg taacttgcgc agcatgcggt tgccaaacaa
     8821 ctgtaccttt taaaccaagc aatgacagac ctgtgttttg ctcagattgc tacaggaaag
     8881 gacaatagtt aattggctaa aaacaatcaa aaacgtgaaa aagcggaacg taacaaagaa
     8941 aatgccgcta agtacaaaaa aagaactggc aataataaat ttagaaataa cagataattt
     9001 ttattaagta taagttataa caccggttta aatttgttaa attaaggcga gtttcctcaa
     9061 agggaaactc gccttaaagt tttattattg tttgtccccg acgtgataac cgcaccatgc
     9121 aaagataaat ggtaaagctc tgccgatttt aagtttttcc ataaaaggca ttttggcaag
     9181 gacaagtgct ccaattaaat cgtccacgtg aacaattaaa tctttattct taaattttct
     9241 gtcaggtgaa atttcgtgag ggtcaaaaac tttgttttca aacttatttg caagccacac
     9301 tgcaagatta acgcctgtcc aaatccctat tcctgttgct gcaattttag gcagtgattt
     9361 attaagtttt ggtattgatt cacagaactt cattcctgca gaaacaaaca atgcaggaat
     9421 agcgacattc ataagttgat aagcaccttc tttaagtttt ctgctttttt tgcgttcatt
     9481 tttatcggca agtcctccta acaatccccc tacaacaccg cctgtgccta atgcaaaaat
     9541 acttccaaaa tcatagtgta tgtctgtgat tttaagaata ttttttagag aattcatttt
     9601 tatttcaggt ttttgccttt ttccgataag cagcaatgga attgctactc ctgcgattga
     9661 tgtgaaaaag gcaattaagc tgtttttctt tttcggggca ttaaaatttg tcctgaaaaa
     9721 ctcatctata tgcttttcat gcgcttgttt ttttatgaag gcaaaacttc catggtaatt
     9781 ttggttttct ccaaaattaa agtttgtagg taattgtgta aaatttattt tagtcatttt
     9841 aattattttt ttataagtaa cccaagtctg ttactattgt aacatctcat aaaacaaaaa
     9901 caagagggtg tatttggcgc gggagatgtt cgggagccaa gactgggtgg aggcgaagat
     9961 gaagacggag acggagggaa agacgaaggc gtggtgcatg cgggcgatga aagtgaagag
    10021 gcaagggttg aaaaatatta aaacatgtgt tacaatctta gtatcagtaa aggatgtggg
    10081 gtgtattatg attacgccgg ttatatcaag attaagcgca ataaattcaa tgaataatgc
    10141 tgcatacggc atgatgcagt cttccaatgc gcttatggga cttatttctt ttgccggcac
    10201 taatcctaat gttgatttag gtaaattaaa ttctgttgaa aaaaatctta tgatgaaaat
    10261 gctcaatgac agttttattt ataagattag tcaagcgcag gaaaaacaac ttaaaaagct
    10321 tgagcaggat aatattaaac attcattttc aacatttgct taattttaat ttaattgttg
    10381 tctttattag tctgcaatag gtgttttcat tagcgtgaaa taaattatcc ccgacagcag
    10441 catacatatt ggaattgtga aaatccatgc tgcaacaatt tttttaacta ttccccattt
    10501 taccgcagag gcttttacca tgctgccgac gccgactatt gctgttgata taatatgagt
    10561 tgtgcttacg ggaaaaccaa agtgcgatgc tgttattatt atacttgctg atgccatttc
    10621 tacggaaaat ccgtgtatgg gtttcatttt tataactttt gagctcattg ttcttataat
    10681 tttccacccg ccggtcattg ttccaagcga caaggtcatc gcgcaggcac atattaccca
    10741 aacagggatt tcaaaacttt tattgtggat tattccttgg gtcattagtg ccaaagttat
    10801 tatccccatt gttttttgtg catcgtttga gccatggctg aaggctaaaa atcctgaaga
    10861 aaataattga agttttctga ataacttatt caatttttcc ggacgcattt tgtggaatat
    10921 acttaacagt acaagaatta ccaaaaaacc tattgtaaag cctaataccg gtgatataac
    10981 cataggtaaa agcactttat ttataacgtt ttggatattt ataacattta aggcagggtg
    11041 atgaagccta agggaaggaa gggtaaggat taattcatag tgcctgtcaa gttcaaaata
    11101 agcatgagaa attgaagcac ccaaaagccc cccaattaga gcatgagaag aacttgacgg
    11161 caatcccagc caccaggtga ataaattcca taaaacagca ccagcaagtg cgcaaaatat
    11221 tacaagctgg gttatgcaag caccttcaac aagccctgca cctattgtat gtgcgacatg
    11281 agtgccaaag aatgcgcctg taaaatccaa agtcgccgca tatataatcg cgtgccttgg
    11341 gctcaaagct tttgttgata ttaccgtgga aatagcattt gccgaatcgt gaaatccgtt
    11401 gatgtattca aaaacgaatg ctattatcag gactaaaata agtaatacta ttgctatttc
    11461 cataattctt aattattctt cagtgctatg gtcaaaactg cgtcagatac gctaaagcat
    11521 ttgtccattg ccctttccaa atccttatat atgtctctta attttataac ttcaatcgct
    11581 tcaaacttcc ctgaaaacag gttgtccata gctctgtaaa gaatattatc tccgtaagtt
    11641 tctatttctt tcattgcttc gcggctttct gttatttctt ttgtttttga aatagttttt
    11701 aaaagcccga cacttctttt aagctcttct gtggcattaa caagggtttt tgcctgttga
    11761 agcatttctt cggtaaaatt ttctattcta tagacattta aattcataaa ggcttgtgct
    11821 gtttttttag ttattttgtt tagcataatc gcaagtgctt gaatgtcttc tctatcaatg
    11881 gg
//
LOCUS       DAOQAU010000052        11340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_6539_length_11340_cov_7.301817, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000052 DAOQAU010000000
VERSION     DAOQAU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6539_length_11340_cov_7.301817"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1268
                     /locus_tag="PKI94_07235"
     CDS             117..1268
                     /locus_tag="PKI94_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNW26563.1"
                     /translation="MVTKEKVSDNDFEALLKQYDYNFKKGDLVKGVVFAYDSEGVIVD
                     IGSKTAALVPSREASVDSNEPVENILEKGQEYEFLIIKEEDDEGRFLLSRRKVDLAYT
                     WKELEKIKEADEIIMGTVASCVKGGVLVDILGVRGFVPSSHLRSKETDLKVGDKIELK
                     ILTLDSQQNNFILSNKKVFTETAEDAKKTVFSQIEVGQVLKGDVVRITDFGAFIDIGG
                     VDGLLPLSQISWRWVDHPSDILKVGDKIDVEVIGVDHDKHRVSLSLKNLENDPWVEAE
                     KQIQEGEQRQGTITRIKHFGAFVEIFQGVEALLPHHELLEYQNSKNCILKVGDKINTT
                     ILKFNPTDKRIALSVKESKEVLKEQETKEVKKPKAKKAKAEEAIEEEKE"
     gene            complement(1344..1721)
                     /gene="rplL"
                     /locus_tag="PKI94_07240"
     CDS             complement(1344..1721)
                     /gene="rplL"
                     /locus_tag="PKI94_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017723037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNW26564.1"
                     /translation="MSNVTSILESIEKLTLLEAAELVKAMEEKFGVSAAAPVAVAAAP
                     AAAAPAAGEEVASEVSVVLASAGANKIAVLKEVRTITGLGLKEAKDLVDAAPKPVKEN
                     IKKEEAEAIKKQLEEAGATVEIK"
     gene            complement(1764..2285)
                     /gene="rplJ"
                     /locus_tag="PKI94_07245"
     CDS             complement(1764..2285)
                     /gene="rplJ"
                     /locus_tag="PKI94_07245"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNW26565.1"
                     /translation="MATKAFKENKIEQIKQKLQKAKVAIVTEYRGLSVEEITKLRRNL
                     QKQDGDYTVTKNTLAKLAAKGTDFEVLTEVFKGPIALALGFKDQVSPAKALSDFIKEV
                     KKGEIIAAALDGKLLSAQEAKALAELPSKEEIYAKMLGCINSPASGIANSMNAVLGQL
                     VRTLDAAKDKLSA"
     gene            complement(2614..3165)
                     /locus_tag="PKI94_07250"
     CDS             complement(2614..3165)
                     /locus_tag="PKI94_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26566.1"
                     /translation="MKKILLIIISLFLAQVVWAYETVLVDFPPNQGWHSVYYGTLDDE
                     AILQYVPVGQTYQNWTKSVIFHSYKNPDLQYNSALFMDRTTSQMELQNSSQMYKYIKY
                     TDADSIATRCVQGNRNVPTQCEIYRVSTSYEGLITMHYINKNQQDFKNTYNMWYQIMQ
                     DIRIYYSYYRTDRILDKATSFEL"
     gene            complement(3137..3847)
                     /locus_tag="PKI94_07255"
     CDS             complement(3137..3847)
                     /locus_tag="PKI94_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNW26567.1"
                     /translation="MKIKEIVDLIEKFAPPELQEEWDNSGWQICYENCFESEVSKVLF
                     CLSVTKDIINQAVEKGCSLIISHHPLFFIPLEFNKNILIYSAHTNLDATKGGTTDTLI
                     SLLEMPPAEKIGSFLRAVELNEEIFLKDFLNLLKDRLNLDMLRVVNKFNVQKIKKIAF
                     CAGSGADFLGEAQKIRADILVTGDIKYHSALESDIILIDIGHFGSEHPVLNTLDKIIS
                     PLGIEVLVADEKDPFDYY"
     gene            complement(3837..5045)
                     /gene="coaBC"
                     /locus_tag="PKI94_07260"
     CDS             complement(3837..5045)
                     /gene="coaBC"
                     /locus_tag="PKI94_07260"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNW26568.1"
                     /translation="MLKGKKVLLGITGGIAAYKMCEFIRMLQKQGAQVKVVTTPSALN
                     FVTKLTLQALSKNKAFVDMFEDENYSPEHISLADDADIFIIAPASANTISKLAMGICD
                     NLLTTVACAFKKPIILAPAMNCNMWENKIIQENLEKLEQSGYHIYPPEEGYLACGYEG
                     AGRLCDLNKLLDKTVQILTAKKKLNGKKIVITAGGTREKIDAVRFISNFSSGKMGLAL
                     ADQAFEQGAEVVLITTVLPRPLRERAGVRGTYQVRLVESALDMQKAVEKEFKTADCLI
                     MAAAVADYRAKKIADKKMKKTSAEEICVDLVKNPDILKEIGLKRRKNQLIIGFCAESE
                     NVLEYAKSKIKTKGCDFLVANDISRSDIGFSSDYNEVYIVDKKLKVKKISRTKKECVA
                     KQILEIVYEN"
     gene            complement(5045..5623)
                     /gene="gmk"
                     /locus_tag="PKI94_07265"
     CDS             complement(5045..5623)
                     /gene="gmk"
                     /locus_tag="PKI94_07265"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNW26569.1"
                     /translation="MENSSAKRKKLFVISGPSGVGKGTVIEGFLKRNPEFKLSISYTT
                     RAKREGEVEGVNYFFRTREEFVKGIENDEFLEWAEFSDNLYGTKRAFIEKCLNNQMDL
                     ILEIDTQGALQVKEKMPKAVLIFIAPPSFEDLEYRLRNRKTESEEAILKRLEFVKTEI
                     KNSAKFDYIVINDEIARAIGELEKIINAERAN"
     gene            5717..6994
                     /locus_tag="PKI94_07270"
     CDS             5717..6994
                     /locus_tag="PKI94_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW26570.1"
                     /translation="MNKNQEITQRNDFGTLSEKTLKDQLKIARSEVIRYANKFLEAEE
                     KWGFINLLLKQLNSIQDKKQLGETICSGLLKLTEAQIVTCCFFNPDKNQINVKCINYK
                     NKKKLSHIEKINKKIKIFVQKTHDVDKIKELFISISEKNMILTPITYNGTFLGYIILM
                     KDEQEFYKQNINFINIFPEHIALILENISNYDELKERNKIKIQFLAGISHEFKTPLNS
                     IIGFSEILKTKTKTKENYKYLENISQSSKHLLALIEDILDVSKSQMKDLELNYTNFRP
                     KEAIKQILMALEENCREKDVELKYTLADVKICADIKRFRQLIYNLVSNAIKFNKKNGK
                     VNVLTYTKDDFFFFEVSDTGDGISKNDYAKIFDFFSQVNRSQLKRQLGSGIGLALCKK
                     ITDAHRGQIDFESRIKKGSRFWFCIPSEKPARK"
     gene            complement(7018..7983)
                     /gene="holA"
                     /locus_tag="PKI94_07275"
     CDS             complement(7018..7983)
                     /gene="holA"
                     /locus_tag="PKI94_07275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNW26571.1"
                     /translation="MSIYFFYGNEEYNIELEIQKLKKKLLDKNFASMNFKTANNPNFA
                     DFVALLRTQPMMFGNMIIVIKVEDYFSKSLEDSQLDEIENALKNNPESLCIVFIAEFE
                     RNENKKIDTRKKFYKILSKYAQIQEFPAIKPYKTDEISNWIIKEAKKREISFEKEATE
                     ALIEQVGNNLRELCLELDKLKLLAYPQTLITKKMVREICISNEDLFSFADFLLKGEKC
                     KALLEFEKLLDKKHSLEILSALHTMLRKWITVKSKSRELPLFEIAKLTGQHEFVVKTT
                     LEKMKNTPLADLVKLKQNLIEAEFKIKTGESVDVVEEIKNSILKI"
     gene            8147..9331
                     /locus_tag="PKI94_07280"
     CDS             8147..9331
                     /locus_tag="PKI94_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNW26572.1"
                     /translation="MTFKEIKNNIFYCGLNHRERKMFDELIPLPQGTTYNSYLIKGSE
                     KTVLIDTMYPPKADEFIQNLANNGIVKIDYIVAQHGEQDHSGALPKLLKIYPEAKILT
                     NPKCKEIIQAMLHIKDEKIQTVEDGEEISIGDKTLKFIHAPWVHWPDTMFTYIKEDKI
                     MFTCDFLGAHLPFENLYAQETCETLKSAKRYYAEIMMPFRKFCQKYVQKLKEFDIEMI
                     LTSHGPIYKNPDFILKAYEEWTSDDCKNKVLIPYVSMYESTKEMVNYLSEKLSEKGIE
                     TLPFDVVNEDLGELAMELVDACTVVFGSSMVLAGPHPAAVTAAYLAGILRPKMKYVSF
                     IGSYGWGGVLTQKLEDALSTVKCEKLEPVIIKGKPKQEDFNLLDRLADEIYQKHKILG
                     LV"
     gene            complement(9436..10413)
                     /locus_tag="PKI94_07285"
     CDS             complement(9436..10413)
                     /locus_tag="PKI94_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNW26573.1"
                     /translation="MNLFLWVLIILAIIYVMKGVIIVQQAEVVIIERLGKFERVLQSG
                     LNFIIPIIEAPRGMQWRVTQKGIDGSTYSYIKERIRIDMREAVYDFPRQNVITKDNVS
                     ISINAIIYFQIIEPKSATYEIQNLSDAIEKLTQTTLRNLVGQLDLQETLVSRDKINQE
                     LRAILDEATNKWGVKVNRVELQDITPPLDIQQAMDKQMKAERDKRANILEAEGLKQAA
                     ILKAEGDKLSQINRAEGDKQSAILRAEGDAQARVINANAEGNAIKIVVDSIANKGKPD
                     QYLIAMKYLDTLKSITAGENNKIVYMPYEATGILSSVDGIKEMFKGTSK"
     gene            complement(10490..10909)
                     /locus_tag="PKI94_07290"
     CDS             complement(10490..10909)
                     /locus_tag="PKI94_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNW26574.1"
                     /translation="MDYWQILIVAGVIFLIIEIFTPMVFFLNFAIACFITAVIALFII
                     DLNYLIPIFIVFSAIFLIFLRPILVKSRDGGKKTGVEEKYIGKTAKVIETVTSNSGVV
                     SIYDERWNARSDSDEEIPAGEEATIVRNESLVLYVKR"
     gene            complement(10965..11165)
                     /locus_tag="PKI94_07295"
     CDS             complement(10965..11165)
                     /locus_tag="PKI94_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNW26575.1"
                     /translation="MGKITKEMGIIDIVQNYPQSLEIFAKYGLGCIGCAAARFENLEA
                     GAKVHGIDPDVMVEEINALISD"
BASE COUNT         3584 a         2151 c         1864 g         3741 t
ORIGIN      
        1 ttaaaatatg tgaagttttt gaatttattt gctttttttt tctcatgctt tgatataatt
       61 aaccaaagat aaagctaaaa aagcttgatt aggacatgct tatcggagaa tggtaaatgg
      121 ttaccaaaga aaaggtttca gataatgact ttgaggcttt actgaaacag tacgattata
      181 attttaaaaa aggcgacctt gtaaaagggg tggtttttgc ctacgattct gaaggtgtta
      241 ttgttgatat cggttcaaaa accgctgctt tggtgccctc aagagaagct tccgttgatt
      301 ctaatgagcc tgtcgaaaat attttagaaa aaggtcaaga atacgaattt cttataataa
      361 aagaagaaga cgacgaagga agattcctgc tttcaagaag aaaagttgac cttgcatata
      421 catggaaaga acttgaaaaa atcaaagaag ccgatgaaat aatcatggga actgtagctt
      481 cctgcgtcaa aggcggcgtc ttggttgata ttttaggcgt taggggcttt gtgccttcaa
      541 gtcacttgag aagcaaagaa accgatttaa aagtcggaga taaaattgaa ttaaaaattc
      601 ttacccttga cagccagcaa aataatttta ttctttcaaa taaaaaggtt ttcacagaaa
      661 ccgcggaaga tgccaaaaag acagtgtttt cacaaattga agtagggcag gtattaaaag
      721 gcgacgtggt aagaattaca gattttggtg catttattga tataggagga gttgacggac
      781 tgcttccgct ttcgcaaatt tcctggagat gggttgacca tccttctgat atactcaaag
      841 taggcgacaa aattgacgtt gaggtcatcg gcgttgacca cgacaaacac agagtaagcc
      901 taagccttaa aaatcttgaa aatgacccct gggttgaagc agaaaaacaa attcaagaag
      961 gcgagcaaag acaaggaact ataacaagaa ttaaacattt tggtgctttc gtggaaattt
     1021 tccaaggcgt tgaagcactt ttgcctcacc atgaacttct tgaataccaa aattcaaaaa
     1081 attgcatact gaaagtcggc gacaaaatca atacaactat tcttaaattt aaccctacag
     1141 acaaaagaat tgcgctgagc gtaaaagagt caaaagaagt cttaaaagag caagaaacta
     1201 aagaagtcaa aaagcctaaa gccaaaaaag ccaaagccga agaagcgatt gaagaagaaa
     1261 aagaataatt gacagtaatt gcctaataat aaaagagcgg gttcaatttt gaacccgctc
     1321 ttatcttttc ttttaaaaaa gagctactta atttcaacag tagcacctgc ttcttcaagt
     1381 tgttttttga tagcttcggc ttcttctttt ttaatatttt ctttaaccgg ttttggagca
     1441 gcatcaacca agtctttagc ttcttttaag ccaagtcctg tgattgttct gacttctttt
     1501 aacacagcaa ttttgttagc accggcagat gccaatacta cagaaacttc gcttgctact
     1561 tcttcgcctg ctgcgggagc tgctgctgca ggagctgcgg caactgctac aggagctgct
     1621 gctgatacac cgaatttttc ttccatagct tttactaatt cagctgcttc caaaagtgtt
     1681 aatttttcaa ttgattctaa aatagatgtt acgttactca tgatttttct cctttatctt
     1741 ttaataattt ctaaactgtt ttactaagca ctaagcttat ctttagctgc atctaatgtg
     1801 cgtaccaact gtccaagaac cgcattcata gaatttgcga tacctgaagc aggagagttt
     1861 atgcagccaa gcatttttgc ataaatttct tcttttgaag gaagctctgc caatgctttt
     1921 gcttcttgcg cggaaagaag ttttccgtcc aaagccgctg caataatttc acctttttta
     1981 acttctttaa tgaagtctga aagggctttt gcaggactaa cctggtcttt aaatcctaat
     2041 gccaaagcga taggaccttt aaaaacttct gtaagaactt caaaatcagt tcctttagct
     2101 gcaagtttag ccaaagtgtt ttttgttacc gtataatcgc catcttgttt ttgaagattt
     2161 cttctgagtt tagttatctc ttcaacgcta agacctctgt attcggttac aatcgcaact
     2221 tttgcttttt gaagcttttg tttgatttgt tcgattttgt tttccttaaa ggcttttgtt
     2281 gccatattgt gctccttttt gttaattatt cttgttttcg accttaagct aaaaaagact
     2341 ttgcgtcttt tctaaaccgc aaaatcttac acatcgaaat attctattcg ggcttaaaag
     2401 ggctcaaata aatgtccctg tgccctttcg gctttcgggc ccttctgccc tgctgccata
     2461 tctgccagtg tttgacctcg gttagcggct ttttgaaaca gatgttccgc ctttaaactt
     2521 gtgtactaac tgtctgcggt tgtaaattaa gactaccaaa agcaaattta aaaatcaata
     2581 gcaaaaaatt aaaatgtgaa ctttgttaca aaattagagt tcaaaagaag tcgctttatc
     2641 aagtattcta tccgtgcggt aataactgta atatatcctt atatcctgca taatctgata
     2701 ccacatatta taagtgtttt taaaatcctg ctgatttttg tttatgtagt gcatcgtaat
     2761 caaaccttca taagaggtag aaaccctgta aatttcacac tgagtaggaa catttctgtt
     2821 tccctgaaca catcttgttg ctattgaatc ggcatctgta tatttaatat atttatacat
     2881 ctggcttgaa ttttgaagct ccatttgtga agttgttcta tccataaata aagctgaatt
     2941 atattgaaga tcaggatttt tataagaatg gaaaataacc gattttgtcc aattttgata
     3001 agtctgtcct accggaacat actgcaaaat agcttcatcg tccaaagttc cgtaataaac
     3061 agaatgccag ccttgatttg gcggaaaatc aaccaaaact gtttcatacg cccatacaac
     3121 ctgcgcaaga aacaaactaa taataatcaa aaggatcttt ttcatctgca actaaaacct
     3181 ctatacccaa aggtgaaatt attttatcca aagtgtttaa cacaggatgt tcacttccga
     3241 aatgcccgat gtcaattaat attatatcac tttcaagcgc actgtgatac tttatgtctc
     3301 ctgtgacaag aatatcggcg cggatttttt gggcttcgcc taaaaaatcc gcgccgctgc
     3361 ctgcgcaaaa cgctattttt ttaatttttt gaacattaaa cttgtttaca actctcaaca
     3421 tatcaagatt aagcctatct tttaaaagat ttaaaaaatc tttcagaaaa atttcttcat
     3481 ttaattctac cgctcttaaa aaacttccga ttttttcagc aggaggcatt tcaagcagtg
     3541 aaatcaatgt gtcggtggtt ccaccttttg tagcatccaa atttgtatgt gccgagtaaa
     3601 tcaaaatatt tttattaaac tcaagcggaa taaaaaacag cggatgatgt gaaattatca
     3661 aagagcaccc tttttcaaca gcctgattaa ttatgtcttt tgttacgcta agacaaaaca
     3721 agactttgga aacctcactt tcaaaacaat tttcataaca aatttgccag ccggaattat
     3781 cccattcttc ctgaagttca ggtggtgcaa acttttcgat taaatcgaca atttctttaa
     3841 ttttcataaa caatctctaa aatttgtttc gctacacatt cttttttggt gcggcttatt
     3901 ttttttactt ttaatttttt atctacaata taaacttcat tataatcgct tgaaaatcct
     3961 atatcagagc gtgaaatatc atttgcaacc aaaaaatcac aaccttttgt tttaatcttg
     4021 gattttgcat attccaaaac attttcgctt tctgcgcaaa aaccgattat taattggttt
     4081 ttgcgccttt ttaaaccgat ttctttcaag atatccggat ttttaacaag gtcaacacaa
     4141 atttcttctg cgcttgtttt tttcattttt ttatcggcga tttttttggc acggtaatcg
     4201 gcaaccgctg ctgccattat aaggcagtct gctgttttga attctttttc tacggctttt
     4261 tgcatatcaa gagcggattc aaccaacctt acttgatatg tacccctcac cccagccctc
     4321 tcccgcaagg ggcgagggag cactgttgta attaaaacca cttcagcacc ttgctcaaag
     4381 gcttggtctg caagggcaag tcccattttg cccgaagaaa aatttgaaat aaatcttaca
     4441 gcatcaattt tttctcttgt tccgcctgct gttatgacaa tttttttgcc gttaagtttt
     4501 tttttagccg ttaaaatttg gactgtttta tccaaaagct tatttaaatc gcataaacgt
     4561 cctgcgcctt catatccgca ggcaagataa ccttcttcgg ggggataaat atgataacct
     4621 gattgttcca atttctccaa attttcttga ataattttat tttcccacat gttgcaattc
     4681 attgcaggag caaggattat cggcttttta aatgcgcatg caacggttgt aagcaagtta
     4741 tcgcagattc ccatagcaag ctttgatatg gtgtttgcac ttgcaggtgc gattataaaa
     4801 atatcggcat catcggcaag tgaaatatgc tcgggcgaat aattttcatc ctcaaacata
     4861 tcaacaaaag ctttattttt gctcaaagcc tgaagggtaa gttttgttac aaaattaagc
     4921 gcgctcggcg ttgttacaac cttaacctgc gctccttgct tttgcagcat acggataaat
     4981 tcgcacattt tgtaagctgc gattccgcct gttattccga gtaaaacttt ttttcctttg
     5041 agcattaatt tgccctttcc gcgttaatta ttttttctag ttcgcctatt gctcttgcaa
     5101 tttcatcatt tattacaata taatcaaatt ttgccgaatt tttaatttca gttttaacaa
     5161 attcaagcct tttcaaaata gcctcttcac tttcggtttt tcggtttctg agcctgtatt
     5221 ccaaatcctc aaacgaaggc ggtgcaataa atatcaaaac agctttgggc attttttctt
     5281 ttacctgaag cgcgccttgt gtgtctattt ccagtatcaa atccatttga ttattgagac
     5341 atttttctat aaaagccctt tttgttccgt aaagattatc agaaaattct gcccattcaa
     5401 gaaattcatc attttctatt cctttaacaa attcttctct cgttctgaaa aaatagttta
     5461 cgccttcaac ttcaccttct cttttggctc ttgtagtata ggaaatagaa agtttaaatt
     5521 cgggattccg cttcaaaaaa ccttcaataa cagttccttt gcctacccct gaaggtcctg
     5581 atatgacaaa taatttcttg cgtttggctg aagaattttc catagacaga ttatacaaca
     5641 aatgaaaata tattgaaaat actataaaat tttgttgtat aataattttg caatttatca
     5701 atccgaggat ttttggatga acaaaaatca agagataaca cagcgaaatg attttggaac
     5761 cttaagtgaa aaaacactaa aagatcaact taaaattgca agaagcgaag ttataagata
     5821 tgccaataaa tttcttgaag ccgaagaaaa atggggcttt ataaaccttc ttttaaaaca
     5881 actgaattca attcaagaca aaaaacaact gggcgaaact atttgcagcg gacttttaaa
     5941 gcttactgaa gcacaaattg tgacttgctg cttttttaat cccgataaaa atcaaataaa
     6001 tgtcaaatgc ataaattata aaaacaaaaa aaaattaagc catatagaaa aaataaacaa
     6061 aaaaatcaaa atttttgtac aaaagacaca cgacgttgat aagattaaag aactttttat
     6121 ctcaatttca gaaaaaaata tgattcttac cccaattact tataatggga cttttctcgg
     6181 atacatcatc cttatgaaag atgaacaaga gttttacaag caaaatataa atttcataaa
     6241 tattttcccc gaacatatcg cccttatttt agaaaatatt tctaattacg atgagctgaa
     6301 agaaagaaat aaaattaaaa ttcaattttt agccggaatt tcccatgaat ttaaaacccc
     6361 gctcaattcc ataataggtt tttctgaaat acttaaaaca aagaccaaaa caaaggaaaa
     6421 ttataaatat ttggagaata tttcacagag ttcaaaacat cttctcgctt taattgaaga
     6481 tattttggac gtttcaaaat cacagatgaa agaccttgag ctcaattaca caaactttag
     6541 accaaaggag gctataaaac aaattttgat ggctttagaa gaaaactgcc gcgaaaaaga
     6601 tgttgaatta aaatacaccc ttgccgatgt taaaatttgc gctgatatta aaagatttcg
     6661 acaacttatt tacaacctgg tttcaaatgc cataaagttc aacaaaaaaa acggcaaagt
     6721 aaatgtcctg acttatacca aagatgattt tttctttttt gaagtctctg acacaggtga
     6781 cggcataagc aaaaacgatt atgcaaaaat atttgatttc ttttctcagg taaacagaag
     6841 tcaacttaaa cgccaactgg gctcaggtat cggacttgcg ctttgcaaaa aaataaccga
     6901 tgctcacaga ggacaaatag attttgaatc cagaattaaa aaaggcagcc gcttttggtt
     6961 ttgtattccg agcgaaaaac cagcccgtaa ataaccctaa aaagttgcca aagcccccta
     7021 aattttcagt atagagtttt taatttcttc tacaacatca acagattcgc ctgttttaat
     7081 tttaaattcg gcttcaatca aattttgttt taatttaacc aaatcggcaa gcggagtgtt
     7141 tttcattttt tccaaagtcg ttttaaccac aaattcatgc tgccccgtca attttgcgat
     7201 ttcaaaaagc gggagctccc ttgattttga ttttaccgta atccattttc tgagcatagt
     7261 gtgaagtgcg gataaaattt caagagaatg ttttttgtcc aaaagttttt caaattcaag
     7321 cagggctttg cacttttcgc ctttgagaag aaaatcggca aacgaaaaca aatcttcgtt
     7381 tgatatacaa atttctctta ccattttctt tgttatcaaa gtctggggat aagcaagcaa
     7441 ttttaattta tccaattcca gacaaagctc tctgagattg tttcccactt gttcaattag
     7501 ggcttctgtt gcttcttttt caaaagaaat ttctcttttt tttgcctctt ttattatcca
     7561 gtttgaaatt tcatctgttt tataaggctt tattgcggga aattcctgaa tttgagcgta
     7621 ctttgaaagt attttataaa atttttttct agtgtctatt tttttatttt cattccgttc
     7681 gaattctgcg ataaaaacaa tacaaagact ttcaggattg ttttttaagg cattttcaat
     7741 ttcatcaagc tgagaatctt ccaatgattt tgaaaaataa tcctcgacct taattacaat
     7801 aatcatattg ccgaacatca tcggctgagt ccttaaaagt gccacaaaat cagcaaaatt
     7861 aggattattt gccgtcttaa aattcattga tgcaaaattt ttgtccaaaa gttttttctt
     7921 caacttttgg atttcaagtt ctatattgta ctcttcgttg ccgtaaaaaa aataaatact
     7981 catacatgta taatacttaa aaaatagtta agggggtaaa tatattaagg attgttgtga
     8041 taagataagt gaataggtga ataagtgaat aaatgaataa gtgcaattat aataaattta
     8101 actattcaac ttatctactg ttcgactttt caactaagga gagattatga catttaaaga
     8161 aattaaaaat aacatatttt actgcgggct taaccaccgc gaaagaaaga tgtttgacga
     8221 acttattccg cttccgcagg gaacgactta caattcgtac ttgattaaag gaagcgaaaa
     8281 aaccgttctg attgacacaa tgtatccgcc taaagcagat gaatttatcc aaaatcttgc
     8341 aaataacgga attgtcaaaa tcgactatat cgtggcgcag cacggcgaac aggaccattc
     8401 tggagctttg cccaaacttc ttaaaattta tcccgaagca aaaattttaa ctaatccaaa
     8461 atgcaaagaa attattcagg caatgcttca tattaaagat gaaaaaattc agacggtaga
     8521 agacggtgag gagatttcta taggagataa aaccttaaaa tttatccacg caccctgggt
     8581 tcactggccc gacaccatgt ttacttacat aaaagaagac aaaataatgt ttacctgcga
     8641 ttttttgggt gcgcatctgc cttttgaaaa tctgtacgcg caggaaacct gcgaaacgct
     8701 taaatctgca aaaagatact acgcagaaat tatgatgcct ttcaggaaat tttgccaaaa
     8761 gtatgtccaa aaattaaaag aatttgatat cgaaatgatt ttgacaagtc acggcccgat
     8821 ttataaaaac cctgatttta tacttaaagc atacgaagaa tggacctcag acgattgtaa
     8881 aaataaagtc ttaattcctt acgtttcaat gtatgaaagc acaaaagaaa tggtgaatta
     8941 ccttagcgaa aaattaagcg aaaaaggaat tgaaactttg ccttttgatg tggttaacga
     9001 agatttgggc gaacttgcaa tggaacttgt agatgcctgt acagtcgtgt tcggctcatc
     9061 aatggttctt gcaggccctc accctgctgc ggttacagcc gcttatcttg caggaatttt
     9121 aagacctaaa atgaaatacg tttccttcat aggctcttac ggctggggcg gagttttaac
     9181 gcaaaaactc gaagatgcgc tttctacggt aaaatgcgaa aagcttgaac ctgttataat
     9241 aaaaggcaaa cccaaacagg aagattttaa cctgttagac cggcttgctg atgaaattta
     9301 tcaaaaacac aaaattttgg gtttagttta gcaaaaaata cacaaagagg cgggattttc
     9361 ttttagaaaa tcccgcccca gtgttacttt atatctgatg ggctaaagcc catcctactt
     9421 tctatttacc atttactatt tactcgttcc tttaaacatc tctttaattc cgtcaacgga
     9481 gcttaggatt cctgttgcct cataaggcat atagacaatt ttattgtttt cgcctgccgt
     9541 aattgatttt aaggtatcaa gatatttcat cgcaatcaga tattggtcag gctttccttt
     9601 attagcaatt gaatcgacaa cgattttaat cgcatttcct tctgcgttag cattaataac
     9661 ccttgcctga gcgtcgcctt cggcgcgtaa aattgccgat tgtttatccc cttcggctct
     9721 atttatttga gaaagtttgt cgccttcggc ttttaatatg gcagcttgtt taagcccttc
     9781 tgcttcaaga atattggcgc gcttatcacg ctctgctttc atctgtttgt ccattgcttg
     9841 ctgaatgtcc aatggcggag ttatgtcttg aagctcgacg cgattcacct tcactcccca
     9901 tttatttgtg gcttcgtcca aaattgctct gagctcttga ttaattttat ctcttgagac
     9961 caaagtttct tgcaaatcca gttgacctac aaggtttctc aaagtagtct gggttaattt
    10021 ttcaatcgca tcggaaagat tttgaatttc ataagttgcg cttttgggct cgattatttg
    10081 aaaatatata atcgcattga tacttataga aacgttgtct tttgtaataa cattttgacg
    10141 gggaaaatca taaaccgctt ctctcatgtc aattcttatt ctttctttta tataagaata
    10201 agtcgagccg tcaatacctt tttgggtaac tctccattgc atacctctgg gtgcttctat
    10261 aatcgggata ataaaattaa gtcctgattg cagaaccctt tcaaatttcc caagccgttc
    10321 aataatgacg acttctgcct gttgaacaat aattacgcct ttcataacat aaattattgc
    10381 aagtataatt aatacccata aaaataaatt cataatgcct ccttttttgg ttgaataggt
    10441 gaaaaggtga aagggtgaat agctttttgc ttttcctcct gccttcttgt catcttttaa
    10501 cgtacaatac taaactctca tttctaacga ttgttgcttc ttcgcctgcg gggatttctt
    10561 cgtccgagtc cgaacgtgcg ttccaccttt cgtcataaat agaaaccacg ccgctgtttg
    10621 aagttaccgt ttcgataact tttgcggttt ttcctatata tttttcttca acgcctgttt
    10681 ttttgcctcc gtctcttgac ttaaccagaa tcggcctgag aaaaatcaaa aatattgctg
    10741 aaaaaacaat aaaaataggt ataagataat ttagatcaat gataaacaaa gctattactg
    10801 ctgttataaa acacgcaatc gcaaagttaa gaaaaaacac cattggagta aaaatttcta
    10861 tgataagaaa aatcactccc gcaacgatta aaatttgcca gtagtccata aaagctcctt
    10921 gtctgaaaaa tcatctgaga ttcttatctt agtcacttaa tcacttagtc acttattaac
    10981 gcattaattt cttctaccat aacatcaggg tcaatgccgt gaactttagc gcctgcttca
    11041 aggttttcaa aacgcgctgc ggcacagcca atacagccaa gtccgtattt tgcaaaaatt
    11101 tccaaacttt gaggatagtt ttgcacgata tcgattattc ccatttcttt tgtgatttta
    11161 cccatagtgt tactcctttt ttgtttttta gattgccgcg tcggagcgtt gctcctcctc
    11221 gcaatgactt atttctaggg ctcggggtat ttacccctcg ccaagaacta tgtcacttca
    11281 cccatagttc cccctttact ttttaatttt tatccttaaa cacattatac aacaaataaa
//
LOCUS       DAOQAU010000053        10990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_6851_length_10990_cov_6.974760, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000053 DAOQAU010000000
VERSION     DAOQAU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6851_length_10990_cov_6.974760"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1566)
                     /gene="murF"
                     /locus_tag="PKI94_07300"
     CDS             complement(199..1566)
                     /gene="murF"
                     /locus_tag="PKI94_07300"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNW26576.1"
                     /translation="MKLTLKEIIEATEAKVLRAEGVNGKFALSTDTRTIKKGEIYLPL
                     KGENFDGEEFIDKAIGAGAIGFFISEQGAVSGEQKISAKLILQVKDTLTAYLELARFC
                     KRKIAPKVVAITGSSGKTTTKEMAFCVACEKFKTHKSELNFNNEVGLCKTILSMPENT
                     QVLIVEMGMRGLGEIELLSKYAEPDISIITNIGTAHIGRLGSVENIAKAKCEIVKHQK
                     KNGILIAHDDKSIKDELIHLFTYSPIHLKHFSLKDTKIVEHKIGYSKFVYKNYQYELN
                     VEGDYNIQNSLAAIEMGLVLGMSEEEIFKGLKKYSPIEKRWEVKEIKGYKIINDSYNA
                     NPESMKASIETFLNLYQNPRLMVLGDMGELGKNEVEYHRQIGDFLIQKIAKTTDVGII
                     TVGKLAGEINLKLKENGISSINFKDNLQTSRYILDNVKIGTTIMLKASRAMKFEEIIN
                     EVNRK"
     gene            1653..4079
                     /locus_tag="PKI94_07305"
     CDS             1653..4079
                     /locus_tag="PKI94_07305"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNW26577.1"
                     /translation="MQVTFDKNSLIIDGKREFIRSGAFHYFRTPGVELARDRFMKLKA
                     AGYNTVDIYLNANYHSKKEGEYDFSGIKDIKKVLETAKETGLYVIIRPGPFINAEVNA
                     GGIPFWVLRKKEVIPRNRIGATYQYSPEYMKFITQWYDAVIPVINNFDNIIAFQIENE
                     YATDEMDEQYMKELYQMARERGITCPIFHNDAYCAGLWADVVDIYACDIYPYINPNQN
                     WKQDHFCFDTIDNIEDSARMFKEDAPIFIAEMQSGWFDKWDGIGYREMRKALGDEHIN
                     IMTKTAVSQGVTMFNHYMAVGGTSWDDLACDEVYTSYEFTAPIDEYGNIQPNLYKAKE
                     INYFLKSFGLTNTGERENLDLGENIYAVRRKDYENNCNWLFVRNMNEEDVLISLHSLP
                     FTLYPYDMKICAEDLGLYACKIEFSQAEIFARIKDENHEVLFMLSDKNSNIYIDKNGE
                     KITISADKNDYENIKFEKDGKSTQFLFISRELANRTWVLDDKVIFNADFVYSNGRIAL
                     SKTTEVAYFDLISGFSKKYFKFQEQPQIIELKDFEISFCSPEIDDGYNYNSWEKTDSK
                     NDLDALCVGKYDEFIWYKTKIPNTVEEVTLSARHLFAMYVNGKEVLNRNSYKYEKLKQ
                     IEETISIPFDKAILYKEQNELTILVQNLGFDKGFSGDTNHPRGLVEFKIKPEINLDFY
                     INDKLSFERGDLRESENPYLTKLSTKFHQEFKENVVFAKYLSMENFPYDRATIFLNGV
                     KIGRFLKNTHHVALQEKFYLPNVFLKPENTIDIVIWEKASNIKTAWDFKFELKNVIMQ
                     VGDQTLHQLY"
     gene            4258..4632
                     /locus_tag="PKI94_07310"
     CDS             4258..4632
                     /locus_tag="PKI94_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26578.1"
                     /translation="MSIIPEFLIKRVYKKGSLKQTDDGVCFDLKNVLGPGFISGFSFV
                     KINDEIFNSEKVKFLTQGVEIKAENISEENPIGFRLGQEGTLILKGAKCLKDGINQII
                     IELMNPEAGKVKLSLTDDFQAA"
     gene            5212..6933
                     /locus_tag="PKI94_07315"
     CDS             5212..6933
                     /locus_tag="PKI94_07315"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNW26579.1"
                     /translation="MKMSKLFVQTLREFPSDAEVMSHKLLVRAGYIRKLTSGIYNYLP
                     LMWRVLTKIENIVREEMNAAGAQELLMPFVQPQELWEESGRWEVYGRELMRLKDRHDR
                     GMCLGPTHEEVITSIARDGLKSYKQLPVNLYQIQSKFRDEIRPRFGLLRGREFIMKDA
                     YSFDVDEAGLDKEYENMTKAYKKIFERCGLETKMVQSDTGAIGGSMSHEFMVIVETDS
                     GESDVFYCNNCDYAANSDHATSILPDAVTDGGFTKAQIIDTPKTKSIEELCQFLKTPS
                     TVILKTLVYIADKKPVFVIIRGDKEAEETKLKNALRATELRIASAAEIQELMQEKGFE
                     AEAGFIGPVGIQGIWTIADESVKNLKNFVLGCNQTDKHMVGANFGAEVKVFEKFYDIR
                     LAQKGEICTQCGAKLDVTRGIEVGNIFKLGTKYSKPMNAVFTDIDGKEKPYVMGCYGI
                     GISRTAAAAVERYHDENGIIWPLEIAPYHVVIVPVNTQDELQMKVAVEIYEKLLAQGI
                     EAVLDDRDERAGVKFKDADLIGFPYRVTVGKTIQEGLVEFKTRANGEMVTLKPEDAIQ
                     KLLKEVL"
     gene            complement(6959..7150)
                     /locus_tag="PKI94_07320"
     CDS             complement(6959..7150)
                     /locus_tag="PKI94_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26580.1"
                     /translation="MNMVLILIIILRLLHLAGVVNLLLGLVYDLIDFIFGVFVQSTFW
                     CIEIKMQQRVKKPNNRRDY"
     gene            7186..7503
                     /locus_tag="PKI94_07325"
     CDS             7186..7503
                     /locus_tag="PKI94_07325"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNW26581.1"
                     /translation="MSDCIFCKIVSGEIPCTKIYENDYLIAIEDINPKAKVHSLIIPK
                     VHVDSFNELEDEKLMAELLKGIKKVCAIKDLKSYRLHVNTGKAEGQIVFHLHIHILSN
                     TIK"
     gene            7570..8409
                     /gene="speE"
                     /locus_tag="PKI94_07330"
     CDS             7570..8409
                     /gene="speE"
                     /locus_tag="PKI94_07330"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNW26582.1"
                     /translation="MNENFYKEITPAGFGIAIKEKEVLFSAQSPFQKVEIIDTDSTLG
                     KILTLDGLMMTTEGDEYFYHEMIAHIPMCTHKNPKTVLVIGGGDGGTVREVLKHDTVE
                     KVVLCEIDGMVIDACKKFLPTIAGELDNPKVEILVQDAIEYIKTKENEFDVVLIDSTD
                     PMGPGEGLFTQEFYNNVKKSLKKGGIVVAQSESPVVNKGEIKKMYDLLKKVFPITSTY
                     TSPIPTYPGGYWAWAFCSEEVQPLSYIDEKRCSDVTKTCKIYNREYHEARFALPNFLK
                     ELL"
     gene            8494..9858
                     /locus_tag="PKI94_07335"
     CDS             8494..9858
                     /locus_tag="PKI94_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW26583.1"
                     /translation="MVMEKIVILGGVAAGPKAAAKIRRLNPQAQIDVYTEDTNISYSA
                     CGLPFYIEGNFDDYKMLLARSPEEFEQQNVHIHLLNRAVKILPDKKQVVILNLETNEE
                     FNVNYDKLIITIGAKPIIPPIQNINLKNVFTLRTMEDGINIKEKALKSKHATIVGGGY
                     IGIEVLEAFVKQGLRVTMVEFAPKIMSIFDDDMSDLIKEHILKRDSHKIEIINSDAVV
                     ELVGKNNVTEVKTKNGLEFETDLVLIATGVRPNVQIAADSGIELGVTGAIKVDKHMQT
                     NIKDIYAAGDCAEKTHIVSKTPVWVPLGSTASKEGRCAALNACGEKDDFEGILGSAVT
                     RYFGFTMSMTGLTEKQALKLGYEPVSVKVTKKDKVGYMPEAKNITIKLIADKKSRKLL
                     GAQAIGCGDADKRVNTVGTGLLSGITVDDFFNSDITYAPPYSPSIDPLLNAAQNLIEK
                     LNKN"
     gene            9912..10502
                     /locus_tag="PKI94_07340"
     CDS             9912..10502
                     /locus_tag="PKI94_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26584.1"
                     /translation="MINKARRADNIIEWIIIGIVAIIFLNLFLGKVYDHFQETVASSK
                     LNKMFQPEQTRFEASSGTNYADPSSQINVPLVGEQGLERYRNGSQRVIDNLMSKDLTG
                     NWLWQRNLAEEATVQCFITRYIDGGSCNYAPFVNGTYGIIINSSYGTTLIPAINPTTP
                     INWWDSSFTNLNLPTTTEQEKIDAVAKIKMKFANVN"
     gene            complement(10499..>10990)
                     /locus_tag="PKI94_07345"
     CDS             complement(10499..>10990)
                     /locus_tag="PKI94_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26585.1"
                     /translation="TRTLGLQIPNRTIKMISFGDDADNSYCIWRPDPTRTDSDHLAFI
                     EQRTWAGGVSSYVSKVFSLLPNSNKLKCEEKITYQNPYTGPEAVWSDLEPKEYENVGS
                     EIIYRGYNSSGYWDGYRNDPESNNNVCYTIGYPNIKLRNIHFRINGLEDPEEIINTIP
                     YER"
BASE COUNT         3860 a         1920 c         2010 g         3200 t
ORIGIN      
        1 tatattgacc aaacattttt gttttaagaa aatcaatata aggcactccc aacaaaaggc
       61 ataaaatcag cccgagtaaa aatgccacta ttatcattat catttatata tgctcctttt
      121 aaaaatttgt tgttttatgt attagtaaat ggtaaatagt aaatagtaaa tggattttta
      181 atttctattc actatttcct atttcctatt cacttcgttt attatttcct caaacttcat
      241 cgcacgcgat gcctttaaca taattgtagt accaatttta acattatcaa gaatgtaacg
      301 ggatgtttgc aaattgtctt tgaaatttat tgaagaaata ccgttttctt taagttttaa
      361 attgatttca cctgccagtt taccgacagt tataattccg acatcagtag ttttggcgat
      421 tttttgaatc aaaaaatcgc ctatttgtct gtgatattca acttcatttt tacccaattc
      481 tcccatatcg ccaagtacca taaggcgagg attttggtat aaatttaaaa atgtttcgat
      541 agaagctttc attgattcgg gattggcgtt gtaactgtcg tttataattt tatagccttt
      601 aatttcttta acttcccaac gtttttctat cggagaatat ttttttaaac ctttaaatat
      661 ttcttcttcg ctcataccca aaacaaggcc catttctatt gccgcaagag agttttgaat
      721 attgtaatca ccctcaacat taagctcgta ttgataattt ttgtaaacga attttgaata
      781 gccgattttg tgttcgacaa tctttgtatc ttttaatgaa aaatgtttca ggtgaatagg
      841 tgaataggtg aataagtgaa taagttcatc tttaattgat ttatcgtcat gggctatcaa
      901 aatgccgttt ttcttttgat gttttacaat ttcgcatttt gcctttgcta tgttttcgac
      961 agagcccaat cgcccgatat gcgccgttcc gatatttgta attattgaaa tgtcaggttc
     1021 tgcgtacttt gacaaaagtt cgatttcgcc aagtcctctc attcccattt ctacaatcaa
     1081 tacttgagta ttttcaggca tggacaaaat tgttttacaa agccccactt cgttattgaa
     1141 attaagctct gacttgtggg ttttaaattt ttcacaagca acacaaaagg ccatttcttt
     1201 tgtggtggtt ttgccgctgc ttcctgtgat tgcaacgact ttgggcgcga ttttgcgctt
     1261 acaaaatcgc gccaattcta aatacgcagt caaagtatct ttcacttgca aaattaattt
     1321 tgcgcttatt ttttgctcac cgctcaccgc tccctgttca cttataaaaa accctatcgc
     1381 ccctgctcca atcgccttat caataaactc ttcaccgtca aagttttcac cttttagggg
     1441 taaatatatt tcaccttttt taatagtgcg tgtatctgta gataaggcaa atttaccatt
     1501 tacgccttca gctcttaata cttttgcctc tgtagcttca attatttctt ttaaagttaa
     1561 tttcattcct aaattttacc tcaaaacggt ctttaatgta aactctttta aaaaaagttg
     1621 ccaaaaagtg aaaaatattg caaaatggtt aaatgcaggt aacttttgac aaaaattcgc
     1681 ttataataga cggcaaaagg gagtttataa gaagcggggc gtttcattat ttcagaaccc
     1741 ccggagtcga gcttgcgcgc gatagattta tgaaactcaa agcggcaggc tacaacactg
     1801 ttgatatata tttaaacgca aattaccatt ccaaaaaaga aggcgaatat gatttttcgg
     1861 gcataaaaga tattaaaaaa gttcttgaaa cagcaaaaga aacaggtctt tacgtaatta
     1921 tccgccccgg accgtttatt aatgcagagg ttaacgcagg gggaattcct ttttgggttt
     1981 taagaaaaaa agaggttatc ccaagaaaca gaatcggtgc tacttatcaa tattcgcctg
     2041 agtacatgaa attcattacc caatggtatg acgctgtaat tcctgttata aataattttg
     2101 acaacataat tgctttccaa atagaaaacg aatatgccac agacgaaatg gatgagcagt
     2161 atatgaaaga gctttatcaa atggctcgcg aaaggggcat cacttgtccg attttccaca
     2221 atgatgcata ctgcgcagga ctctgggcag atgtagtgga catatatgcg tgtgacattt
     2281 acccctacat aaaccctaat caaaactgga agcaggatca tttttgcttt gacaccattg
     2341 acaatattga agattctgca agaatgttca aagaagatgc tccgatattt atagcagaaa
     2401 tgcaaagcgg ctggtttgac aagtgggacg gaatcggcta cagggaaatg agaaaagcac
     2461 tgggagatga gcacataaac attatgacaa aaactgccgt atcccaaggc gttacaatgt
     2521 ttaaccatta tatggcagtg ggcggaacat cttgggatga tttagcttgc gatgaggttt
     2581 atacaagcta cgaattcact gctccgattg atgaatacgg caacattcag cccaatttat
     2641 ataaggcaaa agagataaat tattttctta aatcttttgg gcttacaaac accggcgagc
     2701 gtgaaaatct ggatttgggc gaaaatatct atgcggtcag aaggaaagac tatgaaaata
     2761 attgcaactg gttatttgta agaaatatga acgaagaaga tgttttaatt tctcttcact
     2821 ctttaccctt cactctttac ccttacgaca tgaaaatttg cgctgaagac cttggacttt
     2881 acgcctgcaa aatagaattt tctcaagcag aaatttttgc aaggataaaa gacgaaaacc
     2941 acgaagtctt gtttatgctc tccgataaaa attcaaatat ttatatcgac aaaaacggtg
     3001 aaaaaataac aatttcagca gataaaaacg attacgagaa cataaaattt gaaaaagacg
     3061 gcaaatcaac gcaatttttg tttatttcaa gagaattggc aaaccgtacg tgggttttag
     3121 atgataaggt catctttaac gccgattttg tatattctaa tggcagaata gcactttcta
     3181 aaaccacaga agttgcctat tttgatctaa tcagcggatt ttcaaaaaaa tactttaaat
     3241 ttcaggaaca gcctcaaata atagaactta aagactttga aataagtttt tgttcgcctg
     3301 aaattgatga cggctacaat tataattctt gggaaaaaac agacagcaaa aatgatttag
     3361 atgcgctctg cgtcggaaaa tacgatgaat ttatctggta taaaacaaaa attccaaaca
     3421 cagtagaaga agttaccctg agtgcaaggc atctttttgc aatgtacgta aacggaaaag
     3481 aagttctcaa ccgaaacagc tacaagtacg aaaaacttaa acaaatagaa gaaacaatct
     3541 caattccttt tgacaaagca attttgtata aagaacaaaa cgaattgact attttggttc
     3601 aaaacttggg ctttgacaaa ggattttcgg gtgacacaaa ccacccaaga ggacttgtgg
     3661 aatttaaaat taagcccgaa atcaacttgg atttttacat aaatgacaaa ctttcatttg
     3721 aaagaggaga tttaagagaa agtgaaaatc cttacctgac aaaactttct actaaatttc
     3781 atcaagaatt taaagaaaat gttgtttttg caaaatatct ttcaatggaa aattttccct
     3841 atgacagggc aaccatattt ttaaacgggg ttaaaatagg aagattttta aaaaacactc
     3901 accacgtggc tcttcaggag aaattttatc tgccaaatgt tttcttaaaa cccgaaaaca
     3961 ccattgatat agtcatttgg gaaaaagcat caaacataaa aaccgcatgg gactttaaat
     4021 ttgaattaaa aaatgttata atgcaagtgg gtgaccaaac acttcaccaa ctgtactagt
     4081 ccgatgtact atgttaaccg caaaatgaat taagaatttt ctgcccccct tacattgaca
     4141 tgaaaagacc aacaaaaatt ttaagtacat ttacccctaa agtattgaca cgaatgtgcc
     4201 gataattaat ctgcaaaaga tgtgtgtaca gaacggccaa gtcccaagga gcaatttatg
     4261 agcatcattc cggaattttt gattaagcgg gtctataaaa agggctctct gaaacaaacc
     4321 gacgacggcg tttgctttga tttgaaaaat gttttaggac cgggttttat cagcggtttc
     4381 agttttgtaa aaatcaatga tgaaatcttt aactccgaaa aagttaaatt tctaacccaa
     4441 ggagttgaaa tcaaagccga aaacatttca gaagaaaacc caatcggatt tcgcttaggt
     4501 caagaaggaa cattaatttt gaaaggcgca aaatgcttaa aagatgggat taatcaaatc
     4561 attattgagt tgatgaaccc cgaagccggc aaagttaaac tttctttgac agatgatttt
     4621 caagcagctt agattagtga actgtgagca gttagcggtg aattttattg ttcacacaac
     4681 tgaactttaa tttttataat aaggcggcgc aagatttaaa atcttgcgcc gccttaactt
     4741 actataaaca atcctttaaa ataaagggtt ttggctttaa cctattttat attgtaaaga
     4801 tttgtaacaa aaaatagtat atatgcaatt atttaattga tatatacttt atatatatgt
     4861 aagacgaaac gaacaaacat taaataaaca caaacacccc gtttaaataa aagagactac
     4921 ctttcaaccc cctacacacc ctaccttcct accttcccta acaaaaattt tagctcccgc
     4981 ttcgggggct ttttattagt ttataagttg atgagattat aagttgataa gttttttggg
     5041 ctgaagggct aaaaggctaa tttagttgaa tagtcgaaca gtcgaatagt tgaaaagtta
     5101 aatttattat aattgcactt attcacgtct attcactatt tactatttac ttttcgcctc
     5161 ttttcctttt atgctaaaat aaccatataa gttaaaaaag gaagaattaa tatgaaaatg
     5221 tcaaaacttt ttgtacagac tttgagagaa tttccctcag atgcagaggt catgtcacac
     5281 aaactgcttg taagagcggg ctatataaga aagctcacca gcgggattta caattatctg
     5341 cccttgatgt ggcgggtttt gacaaaaata gaaaatatcg taagggaaga aatgaacgca
     5401 gcaggtgcgc aggagctttt aatgcctttt gttcagcctc aagaactatg ggaagaatcc
     5461 ggcaggtggg aagtctatgg aagagaatta atgcgcctta aagaccgcca cgacagggga
     5521 atgtgccttg gtccgacaca cgaagaagta ataacttcaa tcgcaagaga cggcttaaaa
     5581 tcatacaaac aattacctgt taatttatac caaattcaat caaaattcag agacgaaata
     5641 agaccgcgct tcgggctttt aagagggcgg gaatttatca tgaaagacgc ttacagcttt
     5701 gatgttgacg aagctgggct tgacaaagag tacgaaaaca tgacaaaagc ctacaaaaag
     5761 atttttgaac gctgcggact tgaaacaaaa atggttcaat ccgacacggg tgcaataggc
     5821 ggaagcatga gtcatgaatt tatggttata gttgaaacgg attcgggcga aagcgatgtt
     5881 ttttactgta acaactgtga ttatgcggca aattcagacc atgcaacttc gattttgcct
     5941 gatgcagtaa cagacggcgg ctttacaaaa gcacaaatca ttgatacacc taagacaaaa
     6001 tcaatcgaag aactatgcca atttttaaaa actccttcca cggtcatttt aaaaaccctt
     6061 gtttatatag cagacaaaaa acctgtattt gtaatcatca gaggagataa agaagcagaa
     6121 gaaacaaaat taaaaaatgc acttagggca accgagctta gaattgcttc tgcagcagaa
     6181 attcaagaac taatgcaaga aaaaggtttt gaagcagaag ccggatttat cggtcctgtc
     6241 ggaattcaag gcatttggac aatagcagat gaaagtgtaa aaaatctcaa aaactttgtt
     6301 ttaggttgta accaaacaga taagcacatg gtaggggcaa atttcggtgc tgaagtgaaa
     6361 gtttttgaaa aattttacga cataaggctt gcccaaaaag gtgaaatttg tacccaatgc
     6421 ggagcaaaac ttgatgttac cagaggaatt gaagtcggaa atattttcaa actcgggaca
     6481 aaatattcaa aaccaatgaa tgcggtattt accgatattg acggaaaaga aaagccttat
     6541 gtaatgggct gttacggaat cggaatttca aggacggcag ctgctgcagt cgaaagatac
     6601 catgacgaaa acggaattat ctggccgctt gaaattgcgc catatcatgt ggtaattgtg
     6661 cctgtaaata cccaagacga attgcagatg aaagttgccg ttgaaattta cgaaaaactg
     6721 ctcgcccaag gaattgaagc agtccttgac gacagggacg aacgtgcggg agttaaattc
     6781 aaagatgcgg atttgatagg cttcccttac cgcgtaactg tcggaaaaac aattcaagaa
     6841 gggcttgtag agtttaaaac aagggcaaat ggtgaaatgg tgactttaaa gcctgaagat
     6901 gcaattcaaa aacttttaaa agaggtttta taaatttatg tggaatattt taatcgtact
     6961 aataatcgcg gcgattgttt ggttttttaa ccctctgttg cattttaatt tcaatgcacc
     7021 aaaaagtact ttgaacaaaa acaccaaaaa tgaagtcaat caagtcatag accaaaccca
     7081 acaacagatt gactacgccc gccaaatgca acagcctcaa gataataata agaattaaaa
     7141 ccatgttcat gatataattt tccctgatat aagaaggggg aaatcatgtc tgattgtatt
     7201 ttttgcaaaa ttgttagcgg ggagattcct tgcacaaaaa tttacgaaaa tgattattta
     7261 atcgcaatag aagacattaa tcccaaagca aaagttcatt cgcttattat tccgaaagtc
     7321 cacgtagatt ctttcaacga acttgaagac gaaaagctta tggcagagct tttaaaaggg
     7381 attaaaaaag tttgcgcaat taaagacctt aaatcataca ggcttcatgt taataccggc
     7441 aaagccgaag gacagatagt attccacctt cacatacaca ttttgtcaaa cacaataaag
     7501 taaatagtaa atagtaaata gtaaatagat ttttctgttc actatttgcc aattcaagga
     7561 gaagaaacag tgaacgaaaa tttttacaaa gaaattacac cggcgggttt tggaatagcg
     7621 attaaagaaa aagaagtact tttttcggcg caatcgcctt ttcaaaaagt cgaaataatt
     7681 gatacagatt caactttggg caaaatttta actcttgatg gtctgatgat gaccacagaa
     7741 ggagatgaat atttttacca cgaaatgata gcgcacattc ctatgtgcac acataaaaac
     7801 cccaaaaccg ttcttgtaat aggcggcggc gacggcggta cagtaagaga agttttaaaa
     7861 catgacacag ttgaaaaagt cgttctttgc gaaattgacg gtatggtaat agacgcttgc
     7921 aaaaaattct tgccgactat cgcgggagaa cttgataacc caaaagtgga aattttggtt
     7981 caagatgcta tcgaatatat aaagacaaaa gaaaatgagt ttgatgttgt gttgatagat
     8041 tcaactgatc cgatgggacc gggtgaaggt ctttttacac aagagtttta caacaacgtc
     8101 aaaaaatcgc ttaaaaaagg cggaattgtg gtggcacaat cagaatcccc tgttgtaaac
     8161 aaaggagaaa tcaaaaaaat gtatgattta ttaaaaaaag tttttcctat aacttcaacc
     8221 tacacttcgc cgattcctac atatccgggc ggatattggg cttgggcatt ttgttcagag
     8281 gaagttcagc cactttcata catagatgaa aaacgctgca gtgatgttac aaaaacttgt
     8341 aaaatttaca atagggaata tcacgaagca cgctttgcac tcccgaattt tttaaaagaa
     8401 ttgttataaa aagccaaccc taaaaaattc atttttataa aataaccctt taaaacaatt
     8461 gaccttataa aaaatttaaa ttaaaatcac tttatggtta tggaaaaaat agttatttta
     8521 ggcggagttg cagcgggccc gaaagctgca gcaaaaataa gaagactaaa cccgcaagcc
     8581 caaattgatg tttataccga agacacaaac atctcatatt ctgcatgcgg acttcctttt
     8641 tatattgaag gcaattttga cgactacaaa atgcttcttg caagaagccc cgaagaattt
     8701 gagcaacaga atgttcatat tcacctttta aaccgggcgg ttaaaattct gcccgataaa
     8761 aaacaggttg taattttaaa tcttgaaaca aatgaagaat ttaatgtaaa ttatgacaaa
     8821 cttataatca ccataggcgc aaaaccaata attccgccga ttcaaaacat aaatcttaaa
     8881 aatgttttta ccctcaggac aatggaagac gggattaaca taaaagaaaa agccttaaaa
     8941 tcaaaacacg caaccatagt aggaggaggc tatataggaa ttgaagtact ggaagctttt
     9001 gtaaaacaag gacttagagt tacaatggtt gaatttgcgc caaaaataat gtctattttt
     9061 gacgatgaca tgtcggattt aataaaagaa catattttaa aaagggattc tcacaaaata
     9121 gaaatcatta actcagatgc ggtagttgaa cttgtaggaa aaaataacgt aacagaagtc
     9181 aagaccaaaa acggacttga atttgaaacg gatttagttt taatagcaac cggcgtaaga
     9241 ccaaatgttc aaatagcagc agattcggga attgaacttg gagttacagg agcaataaaa
     9301 gtcgataagc acatgcaaac aaatatcaaa gatatttatg cagcaggcga ctgtgcagaa
     9361 aaaacccata tagtatcaaa aacccctgtt tgggttcctt tgggctcgac tgcaagcaaa
     9421 gaaggcagat gcgcagcact gaatgcttgt ggcgaaaaag atgattttga aggaattttg
     9481 ggctctgcgg ttacaagata tttcggattc acaatgtcaa tgacaggatt aaccgaaaaa
     9541 caggcactaa agctcggata tgaacctgtc agcgttaaag tcacaaaaaa agacaaagtc
     9601 ggatatatgc cagaagctaa aaatatcaca ataaagctca ttgccgataa aaaaagccga
     9661 aaacttttag gtgcacaagc tatcggctgc ggagatgccg ataagagagt aaatactgta
     9721 ggcacaggac ttctatcagg gataacggtt gatgattttt tcaacagcga cataacttat
     9781 gctcctcctt attctccgag cattgacccg ctgctaaatg ctgcacaaaa tttgattgaa
     9841 aaattaaaca aaaattgaca atacattata acaaatgctt aaataaaaaa tgtaaaagca
     9901 ggagatgaaa aatgataaat aaagcaagaa gagcggataa tattatcgaa tggattatta
     9961 ttggaattgt tgcgattatt tttttgaatt tatttttagg caaggtttat gaccattttc
    10021 aagaaacggt cgcatcaagc aaattaaaca aaatgttcca gcccgagcaa acacgttttg
    10081 aagcttcttc cggcactaat tatgccgatc ccagctcaca gataaatgtc ccgctggtcg
    10141 gcgaacaagg gcttgaaaga tatagaaacg gctcacaaag agttattgat aatttgatga
    10201 gcaaagattt aaccggaaat tggctctggc aaagaaatct tgccgaagag gctacagtgc
    10261 aatgttttat aactagatat attgacggag gaagctgtaa ttatgcccct ttcgtcaatg
    10321 gcacttatgg aataatcatt aattcgtctt atggaacaac attaataccc gcaataaatc
    10381 caacaacccc aattaattgg tgggattctt cttttacaaa tttgaacttg ccaacaacaa
    10441 ccgaacaaga aaaaattgat gcagtggcaa aaattaaaat gaaatttgca aatgtaaatt
    10501 aacgttcgta agggatagta ttaataattt cttcaggatc ttcaagtccg ttaattctaa
    10561 aatgaatatt acggagtttt atattagggt atcctattgt gtagcataca ttattgttac
    10621 tttcaggatc attacgataa ccatcccaat atccggaact gttgtacccc ctatatatta
    10681 tttcgcttcc gacattttca tattctttcg gctctaaatc ggaccaaact gcttcaggac
    10741 cagtatatgg attttgataa gtaatttttt cttcacattt aagtttatta gaatttggaa
    10801 gaaggctaaa aactttagat acataactgg aaactccgcc tgcccaagtc ctttgctcaa
    10861 taaatgctaa atggtcgcta tcagttcttg tagggtcagg cctccaaata caatagctat
    10921 tgtcagcatc atcaccaaaa ctaatcattt ttatagttct atttggaatt tgaagcccta
    10981 aagttcttgt
//
LOCUS       DAOQAU010000054        10975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_6870_length_10975_cov_6.569597, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000054 DAOQAU010000000
VERSION     DAOQAU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_10975_cov_6.569597"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..754
                     /locus_tag="PKI94_07350"
     CDS             272..754
                     /locus_tag="PKI94_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26586.1"
                     /translation="MTVGAVNGVYQYTYDYVNQYFNVNVSNARINELMQEFGILPTGN
                     QEEDLRALFNAMYNYYSQQAPVQNNPNTPVPWAPLMQKVGLTPTGDLATDHSAFMVRI
                     DQLQGIAKNGSQEDTTEELVNQSYNVFIPPAKQIQQSPSYDDVVGTINMVLLGLYPGP
                     "
     gene            857..1534
                     /locus_tag="PKI94_07355"
     CDS             857..1534
                     /locus_tag="PKI94_07355"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNW26587.1"
                     /translation="MQFIDFEVKTGKENMQTDSDLLDFAVNNKLKEPIFRLYGWSPAC
                     VSIGRNQSDEFLDKTFLKSKNIDIVKRLTGGRALLHDKELTYSFICPTSFLKNGQSVV
                     NSYKEISQILIDAFEELGIDLAFASKKPQAKFDYCMSISTGADLCFEGKKLIGSAQFR
                     KDGYILQHGSILFDYDKKLLKKIFSENIDENAITSLKQIAPDLDFETLKTCLKESAQC
                     FGKSMIQ"
     gene            1731..2654
                     /locus_tag="PKI94_07360"
     CDS             1731..2654
                     /locus_tag="PKI94_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNW26588.1"
                     /translation="MNFKQRYEKIKETTGKDFEVLESDISNLFQEKSPLNKHLLKFLT
                     SPSKRIRPLLGFLFLRSISNKINKEQYKILLAVELVHNATLIHDDIMDNASVRRKQKT
                     LNTKFDNDLAVVAGDYLLSMSLEKVIETNSSQVLKLFTSAIKQTCLGEIRQYFTKNKI
                     TSIEEYIEKSRQKTALLFEMGVLGGFLLSEKSQDKKLKQTASDFAQNFGIAFQIRDDL
                     MNVLNMENNAENDFQSGVFTAPLIFAQKENKNILKKNVFKEIQKTDAIEKTKTLMNNY
                     FEKAIFSMEKIAPNKYKTAIKELIEILKESI"
     gene            2656..3276
                     /gene="lepB"
                     /locus_tag="PKI94_07365"
     CDS             2656..3276
                     /gene="lepB"
                     /locus_tag="PKI94_07365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNW26589.1"
                     /translation="MDLEEIKSKIKPVLKEAVETAVFVIVMVIIIRFFLGEIRWIPSG
                     SMKPTLLEGDRIFVERVSRFFTTPKRGDIMVFYPPTEKLNMSGIAIFERLTGFFCKDI
                     AYIKRVIGLPGDKILIKEDSDGKYNVFVNGKMLEEKYVMDEYDYIPCTQAMYCGPFTV
                     PKGNYFMLGDNRGNSQDSRFWGFLPKERFVGRAIFLFWPLTRINKF"
     gene            3422..3495
                     /locus_tag="PKI94_07370"
     tRNA            3422..3495
                     /locus_tag="PKI94_07370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3456..3458,aa:Met,seq:cat)
     gene            3604..5556
                     /locus_tag="PKI94_07375"
     CDS             3604..5556
                     /locus_tag="PKI94_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNW26590.1"
                     /translation="MAIKVIKKTVNTDLQSELKEIYEFIGNQKNIADYMQKSLEKYKG
                     LNAIIDDHNNIKMTYDEVLAHITSFASSLQKLGISPKDFVSIFSENNGLHFICNQGII
                     KTGACSVLRGSCAPIKELEYILNHSESKALILSDYKILNSLKNIINSNKNLKFIVIIF
                     PKNEKPSDLNIPVYTFDEFIEIGKNNNFIKPEINPDDNLTMLYTSGTTGNPKGVLLSH
                     KNMLSQILPIEYGLKITVGERSLQILPVWHAYEMTTQIIFFANGFHLHFTTIPYLKDD
                     LLKYDIDIFMSVPRIWEAIRLGIYQKLKQKSKAGYFIFDFAVKNSIRYKIHKMYCEER
                     TPNKITNYNFIMRFYHFIARSFRKPLHIFCTNTLYKKIKSLTGLNFRASFSGGGALSL
                     QDELFYDAIGVNIRIGYGLTETSPDLTLRHADDKNYLCSAGKPIRGTEIKIVDPETFM
                     ELGIFQKGLVLVRGPQIMKGYYKDEQATRKVIDKEGWFNTGDLGWLTVDNHLVLVGRA
                     KETIVLSSGENIEPLPIEEACLQSSYIEQIVLVGQDKNSVGALVVPTKEALEKCGIAV
                     NDLHKGVNLSIKNPDLKELIKKEINNYIKLKPNLASFEKIKNFEILKEAFNQENGLLS
                     QTGKTKRNMIFDKYNDIISKMFTEKK"
     gene            complement(5593..6240)
                     /locus_tag="PKI94_07380"
     CDS             complement(5593..6240)
                     /locus_tag="PKI94_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26591.1"
                     /translation="MFKPVSSVSIVETSKIIGDISKISEVFPKIIGETSSSSDSFVKA
                     AQENFKLEKLSNISDIINFSNFFVSEALKPQRGKNFLVRFINKLGVLSACKYNLEKAF
                     LLDIEVIKDAYGKILAGFGYSEKDGKLFISHLVVDNSKRNNKEIKNILIKIGKRIKNI
                     AEEKNVSEIECMVQEEKDNLIKLYKKAGFTVKLPKEKNDDWTMNVLTKDFGKKYF"
     gene            6407..6913
                     /gene="folK"
                     /locus_tag="PKI94_07385"
     CDS             6407..6913
                     /gene="folK"
                     /locus_tag="PKI94_07385"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNW26592.1"
                     /translation="MTIVYLCLGSNKGDRVGYVQQATRLLGELESCSIIRTSSLYETQ
                     PWLEVDTQWYVNAVIEMKTSLSPISLMEECLRVEKQLGRNRQEEGFQGDRTIDIDILF
                     YGKDVIKQENLEIPHKYLDKRAFALVPMLELNPDFVHPELNKDISQLHEELENPEMVL
                     LYGTKFED"
     gene            complement(6973..8373)
                     /locus_tag="PKI94_07390"
     CDS             complement(6973..8373)
                     /locus_tag="PKI94_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW26593.1"
                     /translation="MANFLVDFFKEPPIKEQMTDEAQIKKTYAHWRLRMFYSCFIGYA
                     IFYLCKKNIAVALPGLSAEFGFTNTELGLLGSSLYLTYGIGKFVNGVIADSADVRKFF
                     PTALILSAIANLCFVLSAFFITPGQASFFGLPANMALLWLFAFFWGANGWFQSAGFPA
                     VAKSLTYWYSNSERGTKWSLWSCSHQVGTFLSVIVSGTIAAHFGWRMAFFVPATLAII
                     AALWLYGRLWDRPQSMGLPCVEKFREEKITEDADCESPEEVDTRTYGQIFKDHILFNK
                     TLWLLAIAYIFVYIIRFGVEDWVIKYLHESKNNSIELATMKLSALPLFGILGTIGAGV
                     ISDKIFKGERAIVNILYLIGVIICITLLKFNTISNIDFVLIALIGVFTYGPQMMIGGL
                     CAVESSSKKVASAATGFTGTFGYVGAFLSSFGTGFMVDKFSWSGALWFWDASALICLL
                     LCIALFVMERKNGGKC"
     gene            complement(8400..9320)
                     /locus_tag="PKI94_07395"
     CDS             complement(8400..9320)
                     /locus_tag="PKI94_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNW26594.1"
                     /translation="MTKKKIIEIVLDTETTGLDYTREKIIEFAAVRLENGKIKDEFQT
                     LINPQQHIRKSSMAIHGINEEMVKDAPTEEEILPKILEFIGDYPIVAHNAIFDYSFLN
                     EASLRLFNKEITNPRIDTQQMFKEVFPDLESHGLENLTKKFNVEFTKHHRAMADTMGL
                     ALAYPGLKKLYLQRYDWQEKQLENVEYLFERYLRIQQCVVAMQSELQDLRTVFKLYFD
                     MGGKPITSQSGETMVYQSRQSFGYDFSAIKDILEEIGAMEKAVKLNTGFIDRLVGGLS
                     LEEEKRNTIKDARQEVSETRNIQIIKQDRR"
     gene            9457..9780
                     /gene="trxA"
                     /locus_tag="PKI94_07400"
     CDS             9457..9780
                     /gene="trxA"
                     /locus_tag="PKI94_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNW26595.1"
                     /translation="MSNAIEINDSTFEAEVLTSSIPVVVDFWAPWCGPCRKLGPVLDE
                     VAQELSGKIKVVKINTDDNFKTAKEYSISGLPSLLVFKNGHAVERLVGLMPKSSIVSN
                     IEKHL"
     gene            9802..10407
                     /locus_tag="PKI94_07405"
     CDS             9802..10407
                     /locus_tag="PKI94_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26596.1"
                     /translation="MINKARRADNIIEWIIIGIVAIIFLNLFLGKVYDHFQETVASSK
                     LNKMFQPEQTRFEPSSNANYADASSQINVPLVGEQGLERYKNGAIRVIQNLINKDLSG
                     NWLWQRNLAEEATVLNIINTLIDNNSAYSSLSGQEAYDLQSKLVQYMINIDLSSKRTR
                     IGQYGAVDYFEADYSVCPWDFSTEDMQIKSIACIKYSFSKN"
     gene            complement(10404..>10975)
                     /locus_tag="PKI94_07410"
     CDS             complement(10404..>10975)
                     /locus_tag="PKI94_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26597.1"
                     /translation="NETELYTHAPVRDQVPVFNNHPVGQRFLDWYGNHLGIAIPQNRP
                     ITGFKYLCTADQKWHENILIKADDEGFYLLDKTINNYGTDYVLHRYSIQGEELHDSFS
                     MSCMQGYNPFDPESPWSPPSDYIEYLGSVGLSRATASNNLLLTLAPLSEHRLRMLFPS
                     GTQFEYQNFSFTTKNGGAVPRNDRAPMAR"
BASE COUNT         3700 a         1900 c         1906 g         3469 t
ORIGIN      
        1 tacttaaact tacaaaagtg ttatatatta tataagtatt ttgtatatac aaaattgctc
       61 aaaaaatgtt taatatttta ataaaaaact ctgaatcctt tatggataaa gggttataga
      121 attgtaacaa attattaaga aagtatatac tttcttgatt tcaatcaatt ttattaaatt
      181 atgttacaaa aataactatt ttaaggtttt tttctaaagt tttggggaat gaaaacgata
      241 tacaaagtat atacttttac gaggtgtgaa aatgaccgta ggtgctgtaa acggtgttta
      301 tcaatatact tatgattatg ttaatcagta ttttaacgta aatgtttcta atgcaagaat
      361 taatgaattg atgcaggaat tcggcattct tcctacaggt aatcaggaag aagatttgag
      421 ggcacttttt aatgctatgt ataattatta ttctcaacaa gcccctgtac aaaataatcc
      481 aaatactcct gttccctggg ctcctttgat gcaaaaagtc ggtttaactc cgaccggtga
      541 tttagctacc gatcatagtg cttttatggt cagaatcgat cagcttcaag gtatagctaa
      601 aaatggttca caagaagata caaccgaaga attagtaaac caatcttata acgtattcat
      661 cccccccgct aagcaaatac aacagtcgcc ttcctacgat gatgttgtcg gtactatcaa
      721 tatggttttg ctcgggctct atcccggacc ttaatttttc tctaacacct ttatctttaa
      781 catctcaggg cgaaaaaagc aaagcttttt tcgccctatt ttttatttgg aagtttattt
      841 gttataaaat taaaatatgc aatttattga ttttgaagta aaaacgggca aagaaaatat
      901 gcaaacggat tcggatttgc ttgattttgc tgtaaataat aagttaaaag aacctatttt
      961 taggctttat ggttggtcgc ccgcttgtgt ttctattggg agaaatcaaa gcgatgagtt
     1021 tttggataaa acttttttga aatcaaaaaa tattgatata gttaaaaggc tcaccggcgg
     1081 cagggcactg cttcatgaca aagaattaac atactctttt atctgcccta caagttttct
     1141 taaaaatgga caaagtgttg tcaattccta taaagaaata tcacaaatat taatagatgc
     1201 ctttgaggaa ttaggcattg atttggcttt tgcatccaaa aagcctcagg caaaatttga
     1261 ttattgcatg tcgatttcaa ccggtgcgga tttatgtttt gaaggcaaaa aattaatcgg
     1321 ctcggctcaa tttagaaaag acggttatat tcttcagcat ggctcaattc tttttgatta
     1381 tgacaaaaaa cttcttaaaa aaattttttc tgaaaatatt gacgaaaatg caattacatc
     1441 attaaaacaa attgcccccg acttagattt tgaaacatta aagacctgct tgaaagaaag
     1501 tgcgcagtgt tttggaaaat ccatgataca ataggataag actaatgcac aagtccattt
     1561 tttacaaaaa tgaccttgta caaagtctta aagacggcta gtgtcgaatg gctaaacggc
     1621 actcctcgtg ccgatttagc cattctcact tttgtaaggt caaccactgc aaaagtgaga
     1681 aaaaacaaat tttctacact tgtgcagtgg tctcaggatt ggagccgaaa atgaatttta
     1741 aacaaagata cgaaaagata aaagaaacaa cgggcaaaga ttttgaggtt ttagaatcgg
     1801 atatctcaaa tttgtttcag gaaaaaagtc ctctaaataa acatttattg aaatttttaa
     1861 cttcaccttc aaagagaatc cgcccccttt tgggttttct tttcctgagg agcatttcaa
     1921 ataaaattaa taaagaacaa tataaaattt tgcttgctgt cgaacttgtc cacaacgcaa
     1981 ccttaatcca cgatgatatt atggacaacg cgtctgtcag aagaaaacaa aaaaccctta
     2041 acactaagtt tgacaatgat ttggccgttg ttgcaggtga ttacctgctt tcaatgtcac
     2101 ttgaaaaagt tattgaaaca aattcctccc aagttcttaa actttttact tcggcaatta
     2161 aacaaacctg ccttggtgaa atcagacagt attttacaaa aaataaaata acatccattg
     2221 aagaatatat cgaaaaatca aggcaaaaaa cagcactcct ttttgaaatg ggtgttttag
     2281 gcggattttt gctcagcgaa aaatctcagg ataaaaaatt aaaacaaaca gcaagcgatt
     2341 ttgcacaaaa cttcggaata gcctttcaaa tacgagatga cttaatgaat gttttaaata
     2401 tggaaaataa tgccgaaaat gattttcaat ccggtgtatt caccgccccg ctcatttttg
     2461 cacaaaaaga aaataaaaac atacttaaaa aaaatgtttt taaagaaatt caaaaaaccg
     2521 atgctattga aaaaacaaaa actttgatga acaattattt tgaaaaggcg attttttcca
     2581 tggaaaaaat cgccccaaat aaatataaaa ccgcaataaa agagttaata gagattttaa
     2641 aagagagcat ataaaatgga tttagaagaa ataaagtcaa aaattaagcc cgtattaaaa
     2701 gaagctgtcg aaacagcagt ttttgtaata gttatggtaa taattataag gttttttctc
     2761 ggagaaatcc gctggatacc ttccggttca atgaagccta ccttgcttga gggcgacagg
     2821 atttttgttg agagagtttc aagatttttt acaactccaa aacgcggcga tataatggtt
     2881 ttttaccctc ctacagaaaa acttaatatg agcgggattg caatttttga aaggttaacc
     2941 ggatttttct gcaaagatat tgcttatata aaaagagtta tagggcttcc gggagataaa
     3001 attttaataa aagaagattc tgacggcaaa tacaacgttt ttgtaaacgg aaaaatgctt
     3061 gaagaaaaat atgttatgga tgaatacgat tatatccctt gcactcaggc aatgtattgc
     3121 ggacctttta ccgtgcccaa gggaaattac tttatgctcg gtgacaaccg cggaaattct
     3181 caggacagcc gtttttgggg atttttaccc aaagaaagat ttgtcggcag ggcgatattt
     3241 ttgttctggc cgctgaccag aattaacaag ttttagtaat ttttgcttgc gtttaaggct
     3301 tgtttatgtg aaaataactt tacaaaaagt ctgtcatccc gaacttgttt cgggatctat
     3361 cccactagca aagctggtta gatgctgaaa taaattcagc atgacaaaat atacaaacta
     3421 tggcaaggta gctcagctgg tgagagcgca cggctcatac ccgtgaggtc gacggttcga
     3481 tcccgtccct tgctatttct ctgtgttatg acgacctcgc gggtgttttt gtcaattctt
     3541 tttattattg tcaaaatcat aatttttggt gtaaaattgt gtaattagaa tattggaata
     3601 attatggcaa ttaaagttat caaaaaaacc gtaaataccg atttgcaaag tgagttgaaa
     3661 gaaatttacg aattcatcgg caatcaaaaa aacattgccg attatatgca aaaatccctt
     3721 gaaaaataca aggggcttaa tgccataatt gatgatcata acaatattaa aatgacttat
     3781 gatgaagtcc ttgcacatat aacttctttt gcctcctcgc tccaaaaact cggtataagt
     3841 cctaaagatt ttgtatcaat cttttccgaa aataacggac ttcatttcat ctgtaaccaa
     3901 ggaattatta aaacaggcgc ctgttcggtt ttaagaggtt cttgcgcccc gataaaagaa
     3961 ctcgaataca tcttaaacca cagtgaaagc aaagcactta ttctaagtga ttataaaatc
     4021 cttaattctc ttaaaaacat cataaattca aataaaaatc ttaaatttat cgttataatt
     4081 tttcctaaaa acgaaaaacc ttccgattta aatattcctg tctacacttt tgatgaattt
     4141 attgaaattg gcaaaaataa taatttcata aaacctgaaa tcaatcccga tgataacctt
     4201 acaatgcttt acacttcggg cactacagga aatcctaaag gagttttgct ttcgcacaaa
     4261 aacatgcttt ctcaaattct tcccattgaa tacggattaa aaatcacagt cggagaacgg
     4321 tccttacaga ttttgcccgt ctggcatgct tatgaaatga caacacagat tatctttttt
     4381 gcaaacggat ttcatcttca tttcacgaca ataccttatt taaaagatga tctgcttaaa
     4441 tacgatattg atatttttat gtccgtacct cgtatttggg aagccataag gcttggaatt
     4501 tatcaaaaat taaagcaaaa atcaaaagca ggctatttta tttttgattt tgcggttaaa
     4561 aacagtatta gatataaaat tcataaaatg tattgtgagg agcgcacccc gaataaaatt
     4621 acaaattata attttataat gaggttttat cattttatag cccgttcatt caggaagccc
     4681 ctccacattt tttgcaccaa cactctttat aaaaaaatta aatctttaac aggtttaaat
     4741 ttcagggcgt ctttttcggg cggcggagca ttatcgcttc aggatgaact tttctacgat
     4801 gcaatcggcg taaacataag aataggatac ggacttacag aaacatcccc tgatttaact
     4861 cttcgccacg cagacgacaa gaattacctt tgctctgcgg gaaaacccat aaggggaact
     4921 gagattaaaa ttgttgatcc tgaaactttt atggaactgg gcatttttca aaaagggctt
     4981 gtgctggtaa gagggccgca gattatgaag ggctattata aagatgaaca ggcaacacga
     5041 aaagtaattg ataaagaagg ctggtttaat accggtgact tgggttggct tactgttgac
     5101 aatcaccttg ttcttgtagg aagagccaaa gaaacaatag ttttatcaag cggagaaaat
     5161 attgagcctt tgcctatcga agaagcctgc ttgcaaagca gttatattga acaaattgtt
     5221 ttggtgggtc aagataaaaa ttcggtcggc gcacttgttg ttcctacaaa agaggccttg
     5281 gaaaagtgtg gtattgctgt taatgacctt cataaagggg ttaatttatc tattaaaaat
     5341 cccgacttaa aagaacttat taaaaaagag attaataatt atataaagct aaaaccgaat
     5401 ttagccagct ttgaaaagat taaaaatttt gaaatattaa aagaagcttt caatcaggaa
     5461 aacggacttt taagccaaac cggaaaaacc aagcgtaaca tgattttcga taaatataat
     5521 gatattattt ctaaaatgtt tactgagaaa aaataacaaa agatggcttg agataatttc
     5581 aaaaaaaata atttaaaaat atttttttcc aaaatctttg gttaaaacat tcattgtcca
     5641 atcatcgttt ttttctttgg gcaattttac cgtaaatccc gcctttttat aaagtttaat
     5701 aagattatct ttttcttctt gcaccataca ttcgatttca gaaacatttt tttcttcggc
     5761 gatgttctta attcttttgc ctatctttat taatatattt tttatttctt tattgtttct
     5821 ttttgaatta tcaactacaa gatggcttat aaaaagtttt ccgtcttttt cggaatagcc
     5881 aaaccctgcc agaatttttc catatgcatc tttaattact tcaatatcca ataaaaacgc
     5941 cttttcaaga ttgtatttgc aagctgataa aacacccaat ttattaataa agcgaactaa
     6001 aaaatttttt cctcgttggg gttttagagc ctcagaaaca aaaaaatttg aaaaattaat
     6061 gatatcagaa atgtttgaaa gtttttctaa tttaaaattt tcttgtgctg ctttaacaaa
     6121 actatctgat gaagatgagg tttctccgat aattttcgga aagacctcgg atattttaga
     6181 aatatcgcca ataattttgg aggtttctac tatactaact gaagaaacag gcttgaacac
     6241 ttacattttg tcctacaaac tacgcaatat ttaaaaaaca aatcaataat aattattgct
     6301 tgttttcatg cgttttttaa aataatttaa tatataactt gtattgttat atattaaaag
     6361 ttttaacatt tgacaatttt accaaagaca ttgtaaaatt aagattatga ctattgttta
     6421 cttatgcttg ggttcaaata aaggagatag agttggttat gttcagcaag ccacaaggct
     6481 tttgggcgaa cttgaaagct gcagtattat aagaacatca tcgctttatg aaactcagcc
     6541 atggcttgaa gttgatacgc aatggtatgt taatgccgtt attgaaatga aaacttcttt
     6601 aagcccgatt tctcttatgg aagaatgttt gagggttgag aaacaacttg gcagaaacag
     6661 gcaggaagaa ggttttcaag gagaccggac tattgatata gatattcttt tttacggaaa
     6721 agatgtaata aaacaagaaa atcttgaaat tcctcataaa tatttggata agcgtgcttt
     6781 tgcgcttgtc cctatgcttg aattaaatcc ggattttgtt caccctgaat taaataagga
     6841 catttctcaa ctccatgaag aacttgaaaa tcctgaaatg gttcttcttt acggaacaaa
     6901 atttgaagat taagttataa aattaaaagg cggcgggctg ctttgcagcc cgccgccttt
     6961 tttatcaact catcagcact taccgccgtt ttttctttcc ataacaaaaa gggcaatgca
     7021 tagtaataaa catattaaag ctgaagcatc ccagaaccac aaggctccgc tccaactaaa
     7081 tttatcaacc ataaatcctg tgccgaaaga agaaaggaag gcaccgacat aaccgaatgt
     7141 tcctgtaaaa cctgttgcgg cagaagcgac ttttttcgaa ctgctttcaa ctgcacataa
     7201 tccgcctatc atcatttgag gaccgtaagt aaacacaccg attagtgcta ttaaaacaaa
     7261 atcaatatta cttatggtat taaattttaa tagggtaatg cagatgatta ctccaataag
     7321 ataaagaatg ttaactattg ctctttcacc tttaaatatt ttatcggaaa taactcccgc
     7381 accgattgtg cctagaatcc caaaaagtgg caatgcactt aatttcatag tggcaagctc
     7441 aatagaattg ttttttgatt catgaagata ttttataacc caatcttcaa caccgaatct
     7501 tattatatag acaaaaatat aggcaatcgc gagtaaccaa agggttttat tgaaaagtat
     7561 gtgatcttta aaaatttgtc cgtaagttct tgtatcgact tcttcgggag attcacaatc
     7621 agcatcttcg gtaatttttt cttctctgaa tttttcaaca caaggcagcc ccattgactg
     7681 aggtctatcc caaagtcttc cgtaaagcca gagcgcggcg attattgcaa gcgttgcagg
     7741 aacaaaaaat gccattctcc aaccgaagtg tgcggctatt gtgcctgaaa ctattacgct
     7801 caaaaatgtt ccgacctggt gggaacaaga ccatagcgac cactttgtac ccctttcaga
     7861 atttgaatac caataagtca aactttttgc aacagcgggg aatcctgcgg attgaaacca
     7921 accgtttgcg ccccaaaaga aagcaaatag ccaaagcaaa gccatatttg caggcaaacc
     7981 gaaaaatgaa gcttgtccgg gtgttatgaa aaatgcactg agaacgaagc agaggtttgc
     8041 aatcgcactt aaaatcaatg ccgtagggaa aaatttcctt acatcggcac tgtctgcaat
     8101 aacaccgttt acaaattttc ctatgccgta agttaagtaa agcgaacttc caagcaagcc
     8161 taattcggtg tttgtaaagc cgaattcggc acttaagccc ggtagtgcaa cagcgatatt
     8221 tttcttgcaa agataaaata tcgcatagcc gataaaacat gaataaaaca ttcttaaacg
     8281 ccagtgtgcg taagtttttt taatctgagc ttcatccgtc atttgttctt taataggtgg
     8341 ttctttaaaa aaatcaacca aaaaattagc cattttcatc tcccgttata ctgattttat
     8401 tatcttctat cttgttttat tatttgaatg ttacgagttt cggaaacttc ctgacgcgca
     8461 tctttaatgg tattgcgttt ttcttcctca aggcttagcc cgcctacaag tctgtctata
     8521 aatcctgtat tgagcttaac cgctttttcc attgcaccta tttcttccaa aatatcttta
     8581 attgcagaaa aatcgtaacc gaaagattgt ctggattggt aaaccatggt ttcaccgctt
     8641 tgtgaagtaa tcggtttacc gcccatatca aaataaagct tgaataccgt gcgcaaatcc
     8701 tgcagttcag attgcatagc aacaacacat tgttgaattc tcaaatatct ttcaaaaagg
     8761 tattccacat tttcaagctg cttttcttgc cagtcatatc tttgcaggta aagttttttt
     8821 aacccgggat atgcaagcgc aagccccata gtatcagcca tagctctgtg atgttttgtg
     8881 aattcgacat tgaatttttt ggttaaattt tcaagtccat gagattccaa gtcaggaaag
     8941 acttctttaa acatttgttg agtgtcgatt ctcgggtttg taatttcttt gttgaaaagt
     9001 cttaaagaag cttcgttaag gaaagaataa tcaaaaatcg cattgtgggc tacaatcgga
     9061 taatcgccta taaattccaa aattttaggc aaaatctctt cttccgttgg cgcatcttta
     9121 accatttctt cgtttatacc gtgaatcgcc atactcgatt ttctaatgtg ttgctgagga
     9181 tttatcagag tttgaaattc atctttaatt tttccgtttt caagtctgac tgcggcaaat
     9241 tcgattattt tttctctcgt gtagtctaag cctgtggttt cagtgtctaa aactatttca
     9301 attattttct ttttagtcat taatctctcc gaccggtaat tgcaaaaaat ctagtttcat
     9361 gcattctatt aaatcgtagc acaaatgtaa aaatattgat atattttttt acccatgata
     9421 aagtaatgtg atacaaggtt ataggagatt tttacaatgt caaacgcaat agaaattaac
     9481 gattcaacat ttgaagcaga agtgttaact tcaagcatcc ctgtagtggt ggacttttgg
     9541 gcaccttggt gcggaccttg tagaaaattg ggaccggtgc ttgatgaagt cgcacaagaa
     9601 ctaagcggca aaataaaagt ggttaaaatc aatacagacg ataattttaa aacagcaaaa
     9661 gaatactcga taagcggatt gccaagcctt ttggttttca aaaacggaca tgcagtagaa
     9721 agacttgtcg gtttaatgcc aaaatcttca atcgtttcca atatcgaaaa acatttataa
     9781 aagcaagctc ggagatgaaa aatgataaat aaagcaagaa gagcggataa tattattgaa
     9841 tggattatta ttggaattgt tgcgattatt tttttgaatt tatttttagg caaggtttat
     9901 gaccattttc aagaaacggt cgcatcaagc aaattaaaca aaatgttcca gcccgagcaa
     9961 acacgctttg agccttcatc taatgcaaat tacgcagacg cgagctctca gataaacgtt
    10021 ccgcttgtag gcgaacaagg gctggaacgg tataaaaacg gagcaattag agtaattcaa
    10081 aatcttatta acaaagactt gagcggaaat tggctctggc aaagaaatct tgccgaagaa
    10141 gctacggtgt tgaatattat aaacaccttg attgataata attctgcata ttcctctctt
    10201 tcagggcaag aggcatatga cctgcaatct aaattagtac agtatatgat aaatatagat
    10261 ttatcttcaa aaagaacccg cataggacag tacggagctg ttgattactt tgaagcagac
    10321 tattcggtat gcccttggga tttttcaaca gaagatatgc aaatcaaaag tattgcctgc
    10381 ataaaatatt cttttagcaa aaattaacgt gccatagggg ccctatcatt tcttggaaca
    10441 gccccgccgt tttttgttgt aaaagaaaaa ttttggtact caaactgagt tcctgaaggg
    10501 aaaagcatgc gcaaacggtg ctctgataaa ggagcaagag tgagcaataa attattagaa
    10561 gcagttgcac ggcttaaacc gacacttcct aagtattcaa tataatctga aggtggagac
    10621 caagggcttt cggggtcaaa cggattatat ccttgcatgc aggacataga aaaactgtca
    10681 tgtaattcct ctccttgtat agaatatcta tgtaaaacgt aatctgtgcc ataattgttg
    10741 atagttttat ctaaaagata aaatccttca tcatcggctt tgattaatat attttcatgc
    10801 catttttgat ctgccgtaca aagatattta aaccccgtta taggtctgtt ttgagggata
    10861 gcaattccaa gatgatttcc gtaccagtca agaaatcttt gccccacagg atgattgttg
    10921 aacacaggga cttggtctct tacaggggcg tgagtgtata attctgtttc gtttg
//
LOCUS       DAOQAU010000055        10389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_7456_length_10389_cov_7.897329, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000055 DAOQAU010000000
VERSION     DAOQAU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7456_length_10389_cov_7.897329"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1099)
                     /locus_tag="PKI94_07415"
     CDS             complement(179..1099)
                     /locus_tag="PKI94_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNW26598.1"
                     /translation="MIKHKKFSDTSTRVLETLKILVKRDSSIQDIINHFEKIDPNNRI
                     YTNEVILKYINTLKVFGFKFIKNKDKYSLLNPPNQISLSENELKTMCFIEKVAKVFPE
                     EKIKQEVNNFLQNLEKRFDDKTRFTASKMIKPDCTVINLDYEKYAKIIKTYEKYCIDK
                     QKIKILYRQPDEKTISVIVEPIEFKYMENKVYFSVYNPFEGKIQNILLEDIIEITQLP
                     VKSNATSILTTVTFKINGRLAENYKLHEGEKVIQIESDGSKIIVNQKEDKIFLLRRLM
                     RYGKFCEVISPKTFRKQMKETIKETLKNYS"
     gene            complement(1092..2033)
                     /locus_tag="PKI94_07420"
     CDS             complement(1092..2033)
                     /locus_tag="PKI94_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNW26599.1"
                     /translation="MQEFLTNNTVNCNLMSLTGYRTLVILGLLMDSPKSPDEINQHFF
                     KNQYIKETFSNDMLRIYINSLREAGCEITRASKTTNNKYVLVSHPFDFDLPEIQLSAI
                     QKLYKSIYEKIEVKQAVEIENLFKKIEMLIKTTKTKEFLANCSIFKNTDKYLIEDLLL
                     HCKNKNQIVIIYKSPRKIEKEIEIIADKLSFKSDKLYLWGHSLTHGEYSYFKVENILK
                     ILTIKLKKEKINNKVTRIIYEVYNPATNNYMIEQNEKIIEKNDKKLVIEKLCENKFET
                     IQKLLYLAKDCKIISPQEIKNEVKNILKSMEKTYKND"
     gene            2162..2767
                     /locus_tag="PKI94_07425"
     CDS             2162..2767
                     /locus_tag="PKI94_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNW26600.1"
                     /translation="MNYIFYVLLVLSIVFGAINGKLPDVVNSIMTGADKAVKISFSLI
                     GLMAFWLGMIKIAEKAGLVELIAKLIKPVTKKLFNEIPPEDSAIGNIAMNFSANALGL
                     TNAATPIGIRAMQDLQKHNKNKKTASNAMCMLLAMNTAGFQLIPASVLAILVGTGFKN
                     PTQIIAPTLLVTSFTFVCAVLLAKLFEKVFPPQEASQGGEE"
     gene            2764..3303
                     /locus_tag="PKI94_07430"
     CDS             2764..3303
                     /locus_tag="PKI94_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HNW26601.1"
                     /translation="MTFQGILNLISLWALPFIILLILTMGIVKKVPIYEVFTEGAKDG
                     FKVAVNIIPYLIAIIVAISMLRASGAIESLAGVLAPLLTKFRIPVDIVPVILIRPLSG
                     AAALGLFSDIATNPHSSDFLTKLAAVMVGSSETTFYVLAVYFGAVGIMKFRYALWVGL
                     LADIIAALASIIVCQWMFL"
     gene            complement(3431..3796)
                     /locus_tag="PKI94_07435"
     CDS             complement(3431..3796)
                     /locus_tag="PKI94_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26602.1"
                     /translation="MFLDFLFKRNECPHDEITPEMKEGYCPDCGEYIQNHWFISRCSC
                     CGLKQRTTIIKGKISSQNKFCRNCGSNSFYVEKLDKINVIDVNYAAVIKQAVRTDRKS
                     FIQCWVEPERFVQLKLLTG"
     gene            complement(3841..4464)
                     /locus_tag="PKI94_07440"
     CDS             complement(3841..4464)
                     /locus_tag="PKI94_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26603.1"
                     /translation="MISVSKIMNTTVNYGLKKPLKTLAKMNLIKKVTKEYRGNNIKFI
                     AGLGIFSIILKDGLGCYLYVKQSLANKKIPEDKRKFVAALDLTNGGLMISSQILAFLT
                     VSNEKVQEKLFGKLFGKTFDNLAKKFYVKINAVKNDTTKMTLQEFEECFKKFKGGTKN
                     IFAFLTSLVAATILAKRVIVPFIATPLADKAKVWMDKKDSKTDKVDK"
     gene            complement(4461..5462)
                     /locus_tag="PKI94_07445"
     CDS             complement(4461..5462)
                     /locus_tag="PKI94_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26604.1"
                     /translation="MNVERVSFKGNAAGTMSGEFSYYAKKFLREKKFEKEIAAEAPKI
                     IQFIRKFGVLTGEVPNILINAIGTGLVAPFFIKYNFLSKSDSDTRTYSALRQPVSAIL
                     AVLMQGGLVLPFDRLMNNMSNKGEWDGHAFNKTGFQDLKYIEKIIKKQNPNLTKAEIK
                     ELAKAKQLEQLITMINHANNNNFVGYILDGKKVKLSKNQLKALMRETLDDLSKKHPDI
                     AKNIESLKHNISKGISIKEFSQKIGKISKETDFAYELIQKHIENVAKNIKGFRAIVGL
                     VVSLSILPVSCAALNWLYPRFMDKFFPSLSKKKKDNTNLLKNIQADTFNGKTKEVSQ"
     gene            5671..6684
                     /gene="gap"
                     /locus_tag="PKI94_07450"
     CDS             5671..6684
                     /gene="gap"
                     /locus_tag="PKI94_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNW26605.1"
                     /translation="MVKVAINGFGRIGRNTLRAAIKEGIYDKIDYVAINDPGLTMKDA
                     AHIFKYDSVMGRFDGKVEVAEDGLIINGKKIKFYAEKDPAMLPWRELGVEVVIESTGF
                     YTDAEKARAHITAGAKKVIISAPAKNEDITIVLGVNCQKYDTNVHHVISMASCTTNCL
                     APVAKVVLEKFGIVKGLMTTVHSYTGDQRLLDAGHKDPRRARAGALNIVPTTTGAAKA
                     VALVLPELKGKLNGFAMRVPTPDVSVVDVVFETEKPVTAEAVNAALKEASQNELKGYL
                     EYVDEPLVSTDFIGSNYSSSVDAALTMTMGDNMVKVISWYDNEMGYSTRLAETTLMIA
                     EKL"
     gene            complement(6749..7471)
                     /locus_tag="PKI94_07455"
     CDS             complement(6749..7471)
                     /locus_tag="PKI94_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26606.1"
                     /translation="MTWRVKMISTLNSMSLSSLFCEDGFEKCNTINIKLKASDITDEF
                     ISCGNYSSLNSKSIDTLVEYKIAKNLQLTSQNLSTENIDYEAEFKEFLNAKGCSNEKE
                     LQALNTTSFKTVGQLRNAVSSIIRTTVSNVLSDDNNLEKYLKDIDNVVSDLSVEELAD
                     FFNSPEEAIKKIIDMLKNFIEKYKDDPSMKEKLSRIKELLKKLEKAQEKTDKDKTSQT
                     EENQKNYIKKNIEKYEKIILLY"
     gene            complement(7755..8918)
                     /locus_tag="PKI94_07460"
     CDS             complement(7755..8918)
                     /locus_tag="PKI94_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNW26607.1"
                     /translation="MTTENNFMLLDRASLRKLSVKDISTSAPDLKNIFEPKSVVIANW
                     LIRWIERDFKSQKIKETDLLPQKAELAYHLGVSIGTMQNAFRYVEDKGYVESKQCIGT
                     MVKDWQNASNYVKKLTSKRDLSAEALKKYFMEKKFKQGQKLPSARSLANTLKTSVNTT
                     RLALEYLASTGIIQHNFMNSNDAQWVLKTTNFAVKNEFSQDFDNVTLVEKIEKELKDY
                     ISKNLKVGDKLPAHEDLSAQFKVSIKTIHDALKSLIKSGILLARRGRYGTTVVKMPYE
                     SEFSPKKETSIFASAQQTAFYNYEKTQNHIKALIAQKYEIGSKLPSISEFSKELDLSP
                     NTIRKAFNNLSKEGYLRFARGRYGGTFVIDIPESTSQSFKWLAVNPKYAEVYK"
     gene            complement(9263..9688)
                     /locus_tag="PKI94_07465"
     CDS             complement(9263..9688)
                     /locus_tag="PKI94_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26608.1"
                     /translation="MQKVIIFIRYLLFFPASFIFAIIISGLVSVIQNFISLISCKFML
                     IAPYGICSNNFEQRTFLADLIFPYLVIYFSILIIPKFKKEAAIIELIFFDLIYLLLSI
                     SFLLKAIININTLETVIIGFLLIITTVLLIQNKTKITIL"
     gene            complement(9709..10011)
                     /locus_tag="PKI94_07470"
     CDS             complement(9709..10011)
                     /locus_tag="PKI94_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26609.1"
                     /translation="MFISFLVFAIKQEKKQNLVTIRDAISHEKQVFGERRQEKKKERE
                     QNAKAAGFACKSECLFDKQTHQINYVIIRNINLHVILNLIQDLTSECQIDAEINSA"
     gene            complement(10019..>10389)
                     /locus_tag="PKI94_07475"
     CDS             complement(10019..>10389)
                     /locus_tag="PKI94_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNW26610.1"
                     /translation="QSRICYMTTKNKETGAKGEILAQEYLKKLGYEIIETNKRFSRAC
                     EIDIIAKDKNTLVFCEVKTRSSDFCGSGFEAITKTKYEHIKTGLFTYLQEHKIYKKFR
                     IDAISIVLTPSVKINHLKNI"
BASE COUNT         3069 a         1689 c         1738 g         3893 t
ORIGIN      
        1 cctgcttcct ctcacaaaac ctgacatcta gtcagctttt gttcggcaga gtctacctct
       61 catccaaact gacatttagt cagtttgtct tcggcagagt gccacaagcc caccgtgtat
      121 ttctaattta tcatttaaaa actttaaaat caagcccttt aggcgattat tcttttaatt
      181 aagaataatt ttttaatgtt tcttttatcg tttctttcat ttgttttctg aaagtttttg
      241 gagatattac ttcacaaaat tttccgtatc gcataagtct tcttaaaaga aaaattttgt
      301 cttccttttg gtttactata attttgcttc cgtcagattc aatttggata actttttctc
      361 cttcgtgaag tttgtaattt tctgccaaac ggccgtttat tttaaaagtt actgttgtaa
      421 gaatgcttgt tgcatttgat tttacaggta actgagttat ttcaataata tcttcaagca
      481 aaatgttctg aattttgcct tcaaaagggt tataaacact gaaataaact ttattttcca
      541 tatacttaaa ttctatcggc tcaacaatca cggaaatagt tttttcgtcg ggttgtcggt
      601 ataaaatttt aattttttgt ttatcaatgc aatatttttc ataagtcttg ataatttttg
      661 cgtatttttc gtaatcaaga ttaataacag tacaatcggg ttttatcatt ttgcttgccg
      721 taaatcgggt tttgtcatca aatctttttt ctaaattttg caagaaatta tttacttctt
      781 gtttaatctt ttcttccgga aaaacttttg caactttttc gatgaagcac atagtcttaa
      841 gctcattttc acttagcgaa atttgatttg gagggttaag aagagagtat ttgtctttgt
      901 tttttataaa tttaaaccca aaaactttta gggtgtttat atatttgaga ataacttcat
      961 ttgtgtaaat cctgttattt gggtctattt tttcaaagtg attaattatg tcttgaatac
     1021 tggaatccct tttaaccaaa attttaaggg tttccagcac tcttgtgctt gtgtcgctaa
     1081 attttttatg tttaatcatt tttgtaagtt ttctccattg attttagaat attttttact
     1141 tcatttttaa tttcttgtgg ggagattatt ttgcaatctt ttgctaaata aagaagtttt
     1201 tggattgttt cgaatttgtt ttcacaaagt ttttctataa ctaatttttt atcatttttt
     1261 tctataattt tttcattttg ttcaatcatg taattatttg tagcagggtt gtaaacttca
     1321 taaattattc ttgtgacttt gttgtttatt ttttcttttt taagttttat tgtaagaatt
     1381 tttaaaatat tttccacttt gaaatacgaa tattccccgt gcgtgaggct gtgcccccaa
     1441 aggtataatt tatcagattt aaaagaaagt ttatctgcta ttatttctat ttctttttct
     1501 attttacgtg gagatttata tataatgaca atttggtttt tgtttttgca gtgtaaaagc
     1561 aggtcttcaa taagatactt atctgtattt ttaaaaatgg aacagtttgc caaaaattct
     1621 tttgttttag tggtttttat cagcatttct atttttttaa acagattttc aatttcaaca
     1681 gcttgtttaa cttctatttt ttcataaata cttttatata atttttgtat tgccgaaagt
     1741 tgtatttcag gaaggtcaaa atcaaacgga tgtgaaacaa gtacatattt attatttgtg
     1801 gttttgcttg ctcttgttat ttcgcagcct gcttcgcgca gtgaatttat atatatgcga
     1861 agcatatcat tagaaaaagt ttcttttatg tattgatttt taaaaaaatg ttgatttatt
     1921 tcatcgggtg atttaggcga gtccattaaa agccccaata ttacaagcgt tctgtatccg
     1981 gtaagggaca ttaaattgca gtttactgta ttatttgtca aaaactcttg catactcact
     2041 ccttataaaa caattctaac gtaaagcaag aaaacttaaa atcctttttt tacaaattat
     2101 tactatatct gtcagagctg ttaaacttga aattttataa aattaatgat taaattgatt
     2161 tatgaattat atattttatg tattgttggt attatcaata gtttttgggg caataaatgg
     2221 aaagttgccc gatgttgtaa attcgattat gacaggggca gacaaagccg ttaaaatttc
     2281 tttttcgctt atcggattaa tggcgttttg gcttgggatg ataaaaatag cagaaaaagc
     2341 aggattggtt gaattaatag caaaactaat aaaacctgta acaaaaaaac tttttaacga
     2401 aattccgccc gaggattctg ccattggaaa cattgcgatg aatttttctg caaatgctct
     2461 cggcctgaca aatgccgcaa ctcctatcgg gataagggca atgcaggatt tgcaaaagca
     2521 caacaaaaac aaaaaaacag catcaaacgc catgtgcatg cttttagcta tgaatacagc
     2581 cggatttcag cttattcctg cctctgtact tgctattttg gtcggaacag gctttaaaaa
     2641 tcccacccaa ataatcgctc ccacactttt ggtaacctct tttacttttg tgtgcgctgt
     2701 tttgcttgca aaattgtttg aaaaagtttt tccgccccaa gaggcttctc aaggaggtga
     2761 agaatgactt ttcagggaat tttgaattta atttctcttt gggcactgcc ttttataatt
     2821 ttgttgatac ttacaatggg aattgtcaag aaagtcccta tttatgaagt ttttactgaa
     2881 ggtgcaaaag acggctttaa agttgcggtg aacataatcc cctaccttat agcaataatc
     2941 gttgctatct cgatgctaag ggcgtcaggt gctatcgaaa gtcttgcagg ggttcttgct
     3001 ccgcttttga caaaattcag gattcctgtt gatattgttc ctgtaatttt aataagaccg
     3061 ctatcagggg ctgcagcttt ggggctgttt tccgatattg cgacaaatcc tcattcaagc
     3121 gattttttaa caaaacttgc tgctgtcatg gtaggaagct cagagacgac tttttatgtt
     3181 ttagctgttt atttcggtgc tgtggggatt atgaagttca gatacgctct ttgggtaggg
     3241 cttttagccg atattatcgc cgcactcgcc tccataatcg tttgccaatg gatgtttttg
     3301 taaaaagttg aatagtcgaa caggtgaata ggtgaagagt tttatattcg ttgcgaagca
     3361 acaaattgaa cttattcact tatttaccta ttcacttatt cttttctatt tactatttcc
     3421 tatttcctat ttatcctgtc aataatttta gctgtacaaa tcgctccggc tctacccagc
     3481 attgtataaa ggattttctg tctgttctta cagcctgttt gataacagcc gcgtaattta
     3541 cgtcaataac gtttatttta tctaattttt caacataaaa agaattgctt ccgcaatttc
     3601 tgcaaaattt gttttgggat gaaattttac cttttataat tgtggttctt tgtttcagcc
     3661 cgcagcatga acagcggctt ataaaccagt ggttttgaat gtattcaccg caatcaggac
     3721 aatagccttc tttcatttca ggggttattt catcgtgcgg gcattcattt cttttgaata
     3781 aaaaatctaa aaacacaaaa agttcctcaa agctgacagt taatttttaa tgggataaat
     3841 ttatttgtca actttgtcgg tcttggaatc ttttttgtcc atccagactt ttgctttgtc
     3901 tgcaagaggt gttgcaataa aaggtacgat aactcttttt gctaaaatcg ttgcggcaac
     3961 aagggatgtg aggaatgcaa aaatattctt agtgccccct ttgaattttt tgaaacattc
     4021 ttcaaactct tgtaatgtca tttttgtagt atcatttttt acagcattta tttttacata
     4081 aaactttttg gcaaggttat caaatgtttt tccgaataat ttaccgaata atttttcctg
     4141 aactttctca ttagaaacag tcaagaaagc taaaatttga gaggaaatca tcaaaccgcc
     4201 gttagtcaaa tctagtgcgg caacgaattt tcttttatct tcgggtattt ttttgtttgc
     4261 caaactttgt ttaacataaa gatagcagcc caaaccgtct ttgaggataa ttgaaaaaat
     4321 tcctaagcct gcaataaact tgatgttatt ccctctgtat tcttttgtaa cttttttaat
     4381 taaattcatt tttgcaagag tttttaaggg ttttttgagt ccgtaattta ctgttgtgtt
     4441 catgatttta gaaacgctta tcattgagac acctcctttg ttttgccgtt gaaggtatct
     4501 gcctgaatat ttttaagcaa atttgtatta tcttttttct tttttgacag gctcgggaag
     4561 aatttatcca taaatcttgg ataaagccag tttagcgcgg cgcagcttac gggcagaatt
     4621 gataaactta cgacaagccc cactattgcc ctgaaccctt taatattttt tgcgacattt
     4681 tctatatgtt tttggattaa ttcataagca aaatcggttt ctttgcttat ttttccgatt
     4741 ttttgggaga attcttttat agaaattcct tttgaaatat tgtgttttaa gctttcaatg
     4801 tttttggcaa tatcgggatg ttttttggat aagtcgtcaa gagtttctct cataagtgct
     4861 ttcagttgat tttttgaaag tttgaccttt ttaccgtcaa gaatatatcc aacaaaatta
     4921 ttattgtttg catgattaat catagtaatt aattgttcaa gttgttttgc ctttgcaagt
     4981 tctttgattt cggcttttgt aagattagga ttttgttttt taatgatttt ttctatatat
     5041 tttaaatcct gaaatcctgt tttattgaaa gcatgaccgt cccattctcc cttattgctc
     5101 atgttgttca ttaatctgtc aaagggaagt accaaacctc cttgcattaa aaccgccaaa
     5161 atggcagaaa caggctgtct taaagctgaa taagtccttg tgtcgctgtc tgattttgaa
     5221 agaaaattgt attttataaa aaacggtgca actaaacctg ttccgattgc atttatcaaa
     5281 atgttgggga cttctccggt taatactccg aattttctta taaattgtat aattttaggg
     5341 gcttctgctg caatttcttt ttcaaatttt ttttcacgaa gaaacttttt cgcataataa
     5401 gagaactctc ctgacattgt gccggctgcg tttcctttaa aactaaccct ttctacgttc
     5461 attgctattt cccaaccaat atattatgaa gtttccttcc aattatataa acgtaaataa
     5521 ccctcaaaaa atgcttttat aacaaggttt ttggttattt ggtttacatt tctttacatt
     5581 tattttcttg caatattctg cttgataaag ttttgtgttt ggggtataaa atagttgttt
     5641 ataaccgtac ataaaataag gagaaagaca atggtaaagg tagcgattaa cggctttgga
     5701 agaatcggcc gtaacacatt aagagcagca atcaaggaag gaatttacga caaaattgat
     5761 tatgttgcaa taaatgatcc gggtttaaca atgaaagatg cagcccatat ttttaaatat
     5821 gactctgtaa tgggaagatt tgacggaaaa gtcgaagtag cagaagacgg acttataatt
     5881 aacggtaaaa aaatcaaatt ttacgctgaa aaagaccctg caatgcttcc ttggagagaa
     5941 ttaggcgttg aagtcgtaat cgaatcaaca ggtttttaca cagatgctga aaaagcaaga
     6001 gctcacatca cagcaggtgc taaaaaagtt atcatctcag ctcctgccaa aaacgaagat
     6061 ataacaatcg ttttgggtgt aaattgccag aaatatgata caaacgttca ccacgtgatt
     6121 tcaatggcat cttgcacaac aaactgttta gctcctgtag ctaaagttgt tttggaaaaa
     6181 ttcggtatcg taaaaggttt gatgacaaca gtccattcat atacaggtga tcaaagactt
     6241 cttgacgcag gtcacaaaga tcctcgtcgt gccagagcag gagctttaaa tatcgttcct
     6301 acaacaaccg gtgctgctaa agctgtcgca cttgttcttc ctgagttgaa aggaaaattg
     6361 aacggctttg ctatgagggt cccaacacct gacgtttcag ttgttgatgt tgtgtttgaa
     6421 acagaaaaac ctgtaacagc agaagcagtt aacgcagcct tgaaagaagc cagccaaaat
     6481 gagcttaaag gctatttgga atacgttgat gaaccgcttg tttcaaccga ctttatcggt
     6541 tctaactatt cttcatccgt tgatgcggct ttgacaatga caatgggcga caacatggtt
     6601 aaagtaatat cttggtatga caacgaaatg ggctattcaa cccgtttggc tgaaactact
     6661 ttgatgattg ccgaaaaatt atagacagct taaaaataac aaacaaacaa aagcgggcgg
     6721 ctctgaaaga gccgcccgct tttgtttgtt aatataataa aattattttt tcatactttt
     6781 caatattttt tttaatataa tttttttgat tttcttctgt ttgggaagtt ttatccttat
     6841 ctgttttttc ttgagctttt tctaattttt ttaataattc ttttattcta cttaattttt
     6901 ctttcatgct tgggtcatct ttatactttt cgataaagtt tttaagcata tcaattattt
     6961 ttttaatggc ttcttccggt gaattaaaga agtctgctaa ttcttctaca gataagtctg
     7021 aaacgacatt atctatatcc tttaaatatt tttctaaatt gttgtcatcg gataatacat
     7081 tagaaacagt tgttcttata attgaactta ctgcattcct taattgaccg actgttttaa
     7141 aagatgttgt attaagagct tgaagctctt tttcatttga gcatcctttg gcatttaaaa
     7201 attccttaaa ttctgcctca taatcaatat tttctgtact taagttttga gaagtgagtt
     7261 gtaaattttt ggcaatttta tattcaacta atgtatcaat cgattttgaa tttaaagaac
     7321 tgtaatttcc gcatgaaata aattcatcag taatgtcaga tgcctttagc tttatattta
     7381 tcgtattgca cttttcaaaa ccatcttcgc aaaataaaga ggataagctc attgaattta
     7441 aagtagaaat catttttact ctccaagtca tggttatact atcaatcaat agtataaatt
     7501 ttatcctcta aaaacaggaa aaattgagca tgctcattta caaaaaaggg gaaagagcaa
     7561 acagtaaaat acaagaagaa aacttataaa ctcataatct tataaacttt tcagcttttc
     7621 acctatagat aagatcccga tacatcgcaa gcgatactct cccttgtaac cctcgacaag
     7681 tctcgggaac aagaagagaa gacaagttcg ggatgacata tttctaaggc tcgctttgct
     7741 tgcctagaac tatgtcattt ataaacttct gcatacttag gattaaccgc aagccatttg
     7801 aatgattggg aggtagattc agggatatct attacaaaag tacccccgta gcgtcctctt
     7861 gcaaagcgca gataaccttc ttttgacaaa ttattgaaag cttttcttat tgtgtttggg
     7921 ctcaaatcaa gttctttgga aaactctgaa atggaaggaa gttttgagcc tatttcgtat
     7981 ttttgggcga taagtgcttt tatatgattt tgagtttttt cgtaattgta aaaagctgtc
     8041 tgctgtgctg atgcaaagat tgaggtttct tttttgggcg aaaattcgct ttcgtaaggc
     8101 atttttacga ccgttgtgcc gtagcggcct cttcttgcaa gcagaattcc gcttttaata
     8161 agtgatttaa gcgcatcgtg aattgttttt atgctcactt taaactgtgc ggagaggtct
     8221 tcgtgggcgg gtaatttatc tccgacttta agattttttg aaatataatc tttcagttct
     8281 ttttctattt tttcaacaag agttacgttg tcaaaatctt gggaaaattc atttttaacg
     8341 gcaaaatttg ttgtttttaa aacccactgg gcgtcatttg aattcataaa attgtgttga
     8401 atgatgcctg ttgaggcaag gtattcaagg gcaagccttg ttgtatttac cgaagttttt
     8461 aaagtatttg caagagacct tgccgaagga agtttttgtc cctgtttaaa tttcttttcc
     8521 ataaagtatt ttttaagtgc ttcggcactc aaatcacgct ttgaggtaag ctttttaacg
     8581 taatttgaag cattttgcca gtctttaacc attgtgccta tacactgttt ggattctaca
     8641 taacctttgt cttctacgta cctgaacgca ttttgcattg ttcctatgct gacgcctaag
     8701 tgataggcca gctctgcttt ttgaggcaga aggtctgttt cttttatttt ttgtgattta
     8761 aaatcacgtt caatccagcg gataagccaa tttgcaatta cgaccgattt gggttcaaaa
     8821 atatttttta aatccggcgc agaagtcgaa atatccttta cgctcaattt tctcaggctt
     8881 gctctatcca agagcataaa attattttcg gttgtcatat taacctccac acttttaatt
     8941 atacagttaa tataaaaaaa catacaacat ttttttgctg tataacaaca aaaaatgtta
     9001 caattcttat tagtgatgct tttaaattaa tttttttgtg aacctacttt cttgtgtcga
     9061 caagaaagta ggcaaagaaa ctcccgaccg gatctgcgtt cgttaactat cattaaactc
     9121 aactttaggc gtgctaagtg acgcaagcct ttgcctttgt ttcgttaagt gttagttact
     9181 cactacgata tggtcggaaa tatggcacgt cattgcgagg gcaagcgaag cgatctaaaa
     9241 taacagtggg ttgaaaatat gactacagta ttgttatctt tgttttgttt tgtatcagta
     9301 atacagttgt gataattaat aaaaaaccta taattacagt ttccaatgta ttaatattaa
     9361 ttatggcttt taataaaaaa gatatactta ataataaata aattaaatcg aaaaatatta
     9421 attcaatgat ggcagcttct tttttaaatt tgggaattat taatattgaa aaatatatta
     9481 ctaaatatgg aaaaatcaaa tccgccaaaa aagttctttg ttcaaaatta ttagaacaaa
     9541 ttccgtaagg tgctatcaac ataaacttac atgatattaa agaaataaaa ttttgaataa
     9601 cagatactaa gccagatatt atgatcgcaa aaataaaact agcaggaaaa aacaataaat
     9661 atctgataaa tataattact ttttgcattt ctcatttacc ttcttttgtc atgctgaatt
     9721 tatttcagca tctatctgac attcgcttgt gagatcctga atcaagttca ggatgacgtg
     9781 taaatttatg tttcttatta taacataatt tatttgatga gtttgcttat caaataaaca
     9841 ttcacttttg caggcaaagc ctgctgcttt ggcattttgt tcgcgttctt ttttcttttc
     9901 ttgccttctt tcgccgaaaa cttgtttttc gtgcgaaatg gcgtcacgaa tagtgactag
     9961 gttctgtttc ttttcttgtt taatcgcaaa aacaagaaaa gaaatgaaca tgaaaaaact
    10021 atatattttt aaggtggtta attttaaccg aaggggttaa tactattgaa attgcgtcaa
    10081 tacggaattt tttataaatt ttatgttcct gaaggtaagt aaaaagtcct gttttaatgt
    10141 gttcgtattt agttttggtg attgcttcaa atcccgagcc gcaaaaatca gatgaacggg
    10201 ttttgacttc acaaaaaacg agcgtatttt tgtcttttgc aataatatca atctcacagg
    10261 cacgcgaaaa ccttttgttg gtttcaataa tttcataacc cagtttttta agatattctt
    10321 gggctaaaat ttcacccttt gcacctgttt ctttgttttt ggttgtcatg taacaaatcc
    10381 ttgattggt
//
LOCUS       DAOQAU010000056        10255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_7613_length_10255_cov_6.921765, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000056 DAOQAU010000000
VERSION     DAOQAU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7613_length_10255_cov_6.921765"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PKI94_07480"
     CDS             <1..1155
                     /locus_tag="PKI94_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26611.1"
                     /translation="LKSFSDDEIYNVITAAAKVTLADMSASCLYILSETDLVSAEVLS
                     MRIPFEGNIKFLECNKYVQEPILSADFNILPNLAMQITPTSIGSAIYNFSDFSLKFNL
                     AGQIEGEEGVAVSDGTYPVVNIGGIDVEITCDFVKKISVVIGLTLIIPTIIIAFALNK
                     FLEQEKTKLSDLNTKIQERTIEIKKYSDLSSGAAFDLNAAINSITVSNRTKLFYYEAV
                     GMSIPSKLWINYYTTNSTGGVDIKGRSNDVGSIYTFYKGIKQLVNNSNVRLYKLELAS
                     DSVDDLVSDSSSGPKFYDFEITNMTEAELNPQAATSTQNQAAGQNQTNQQKPLFDFSK
                     PLFGSKNDSNQTQNNPQSATPAPVPTPPAAPPASPPADKLPQNLQKIDKF"
     gene            1165..1728
                     /gene="pilO"
                     /locus_tag="PKI94_07485"
     CDS             1165..1728
                     /gene="pilO"
                     /locus_tag="PKI94_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNW26612.1"
                     /translation="MQRYKDAVLYIVFMIMAFAYVIFKMQPTVVELISVEKEISAKTT
                     ELADLERKLETLKAGSMEKQVKIAGQAKKIFKPEISGLDVEGASTVLFDDIIEMAKYN
                     GIKFYSVEYVYNPPEDEFVKGAADKYNVCQVSMQMVADYSDLSSFLKDIYKYPYLLNI
                     DKIELVPYPKNKKILMINMQLRLYTEK"
     gene            complement(1731..2543)
                     /locus_tag="PKI94_07490"
     CDS             complement(1731..2543)
                     /locus_tag="PKI94_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNW26613.1"
                     /translation="MSIITAPVSKMEELKNLYIELSAKSCNQRCKHCYIDFPVSRNVK
                     DFISVDKIKQAIADTKEQNLECIYLTGAEPMTHPEFNAILRICLKRADVCVCTNASFI
                     NEKKARFLKKVEEEGGNEIFFKLSIDHYNEIKNDDIRGRGSYRQTVNAIKSLIKYDFN
                     PILSIVNYYREDEKTLLHEFKKVCEKIGFDTVLGNFEINNYYNKYSPQHGVVENVSED
                     IICKNSRILTEKGVYVCPFLTNDHRGRMGNSFSDYSKKMPLETNFCYICNQK"
     gene            complement(2685..3911)
                     /locus_tag="PKI94_07495"
     CDS             complement(2685..3911)
                     /locus_tag="PKI94_07495"
                     /inference="COORDINATES: protein motif:HMM:NF018540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HNW26614.1"
                     /translation="MNSTQIIKNTELQLQKQFEIIDEIRDFNQKKVLDAFVENKVAAE
                     HFMTVSGYGHDDIGRETLDKVFAHAFKAQKAVVRNHFVSGTHALACCLFGNLRHGEKL
                     ISVAGSPYDTMEEVIGTRGDKRASLIGHGVTYEEIPLKNDMEVDFELLEKTIDKMCKM
                     VLIQRSRGYSLRKSLDISTIEKIIHIVKSKNPNCICFVDNCYGEFCEKKEPLEVGADL
                     IAGSLIKNPGGGIVETGGYIAGKEEFVEQAAMRLTAPGIGSEGGAMLNQSRLIFQGFF
                     MAPSIVSEALKGAVLAAKVFEEMGFISTPKFDEKRTDIIQTITFNAQKPLEDFCKTIQ
                     ELSPVNSYVTPIPDYIPGYEDSVIMAGGTFIEGSTIELSADGPIRPPYAAYMQGGLNY
                     AHVKIVIQGIINKLNN"
     gene            4001..4723
                     /locus_tag="PKI94_07500"
     CDS             4001..4723
                     /locus_tag="PKI94_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNW26615.1"
                     /translation="MRNFILILIFLCLTGIAFAQNYNVYQPGDFDKNKLYDADEKILF
                     ILDFSGSMDEYLGKEKKVTLMLKTMSQILPFIDKKKQVGLRVYGNRQGFTQYDACKAS
                     SLLVPITPASAAEIEMKLAKTHPKGMTPITYSLNKAVYSDFLGFSGQKHIILLTDGGE
                     NCDESPCLWAMELIKTRSDVKIDVIAFNIDDQDDLDQLKCAALVTTGKFYSAKTAAEL
                     VDSLRKSLSIRKDVEAKIILNK"
     gene            complement(4728..5123)
                     /locus_tag="PKI94_07505"
     CDS             complement(4728..5123)
                     /locus_tag="PKI94_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26616.1"
                     /translation="MQINRVNSPSFGTKIPTINAIELVVGRSLTYDGIEPAMQCAEKI
                     IGKLNECTIDIPYIISCCKEVLIKQFPVLEKVEADANRFFKCRHSASSIFDWVNNQVK
                     IIGAKELDVVPIKTTSGPADVVKALRMIF"
     gene            5327..5665
                     /locus_tag="PKI94_07510"
     CDS             5327..5665
                     /locus_tag="PKI94_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26617.1"
                     /translation="MKEKIIKKYLKENGKIPFDDWYYKLDKPLRNTIAIRLTRVLSNL
                     YGKHRNLPNGIIELKFDNGLRLYFTEINNEILLLLLGGNKQRQSDDINKAQEYLKDYQ
                     ERTKKHDRKK"
     gene            5649..5978
                     /locus_tag="PKI94_07515"
     CDS             5649..5978
                     /locus_tag="PKI94_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW26618.1"
                     /translation="MTAKNKKLIDTFPTFDISQELIDDETALDYLQQILTLYVEDGRE
                     DVFMNCLKPLIKAQGSVSDFAKKTGISRTYFYKLFNNKITPELPTLIRIISNLGYKMS
                     FNLEKYA"
     gene            complement(5975..6667)
                     /gene="rph"
                     /locus_tag="PKI94_07520"
     CDS             complement(5975..6667)
                     /gene="rph"
                     /locus_tag="PKI94_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNW26619.1"
                     /translation="MRKNRKNDELRKIKFTKNYTKHALGSVLVEFGDTKVITTASVEF
                     EKPRWMPRDAKSGWVTAEYSLLPSSTSTRCKRERTSVSGRTQEIQRLIGRSLRACVDL
                     EKMPDITIIVDADVIQADGGTRTASICGGFLALQESVKKLIKDKKLEQNPIIEPLGAI
                     SAGIVEGEIRLDLNYEEDSSAQADANLVMTKSGKIIEFQTTAEGHPFSEKQMLEVLKI
                     AKKGIKEIIEKY"
     gene            complement(6720..6950)
                     /locus_tag="PKI94_07525"
     CDS             complement(6720..6950)
                     /locus_tag="PKI94_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26620.1"
                     /translation="MFLCKNCKSIDKFELMFSPDYKGEKKFSQKYNKKNEIEITVDGY
                     TFIPDLMFMNEHAVCRYCGQIYMWDYGEVNSK"
     gene            complement(7021..7947)
                     /locus_tag="PKI94_07530"
     CDS             complement(7021..7947)
                     /locus_tag="PKI94_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26621.1"
                     /translation="MARIKNSVFGIFIQATKLYFTNFTKFFKYMAFPVFGQILGIIIA
                     LLSSYYYTLNVSNLIAQGGIFNNFSIIFLVLILITLPGLFIFMKAFWDYLVSYGAINS
                     MVENLVKSGKVYDFAAHNEVITRRTPAFVGLWLLFALFTIISMFPLFWLVAGILFVYF
                     VLVFQVFTFEPDKSPVGCFKKSFHIIKGNFWKTCGLMSLLWIFTYWVLPETVKFLFDA
                     AKIIAFLAIPLDGWASQFPIDEINKILTQSPTAYQITSLLIAQFVVSAFLGYAVICLT
                     LPLRSICWALWYKALNKGEAKLDKKILDRAEG"
     gene            8026..8658
                     /locus_tag="PKI94_07535"
     CDS             8026..8658
                     /locus_tag="PKI94_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNW26622.1"
                     /translation="MTESYKDLIKSFTKQDYENRVNLHIHSTFSDGGVNAVELVEKAK
                     AKGLKYISITDHNTLNAYLQTAILNEEIIIPGIEFDCWFGTVFLHMLGYGVDVNNKKL
                     QSLCAKTKRGTEADWVRIFSFKHPKRVIDAIHEAGGIAVLAHPACCFTFSLEKLVKNL
                     MKFGLDGIEVYYPYERHRKIVKFHTVKTVEKIADKLGLIKTGGTDEHGSL"
     gene            complement(8738..10099)
                     /gene="glnA"
                     /locus_tag="PKI94_07540"
     CDS             complement(8738..10099)
                     /gene="glnA"
                     /locus_tag="PKI94_07540"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNW26623.1"
                     /translation="MSDFTHVALNNKTFSEEEIKKLIKENNIRFIQLQFVDINGQVKN
                     MAVPSEHIEKVLANEIMLDGSSIKGFRSIETSDMFFYPDKNSFQILPWTTNDNAKSAR
                     LICDIYNPDGTPFEGCPRCNLKRVMKEAEKLGFSMNLGPEAEFFLFEKKDGKITTETQ
                     DKAGYYDVGPEDLGENVRADIVSALQKMNFDIEAAHHEVADGQHEIDFKYADILTTAD
                     NVVTFRIAVKAIAAQHGLHATFMPKPIFGINGSGMHCNISLFKNGKNAFLDEKKEYQL
                     SDAALFAIGGLLKHVKSITAITNPTVNSYKRLVPGYEAPVYLAWSLANRSALLRVPAK
                     RGNATRVELRSPDPSCNPYLAFAAILESCLDGIRNKIDPPAPVESNIYHLTSKERKKQ
                     RIDSLPGSLAESLEQLDKSLVARAALGEHIFHEFMSSKKKEWDSFRTYVSGWEINKYL
                     ERY"
BASE COUNT         3209 a         1859 c         1783 g         3404 t
ORIGIN      
        1 ttaaaatctt ttagtgatga tgaaatctat aatgtaatta cagctgcggc aaaagtcact
       61 cttgctgata tgtccgcatc ttgcttatac atcctttctg aaaccgattt ggtgagcgcg
      121 gaagttttat ctatgagaat tccttttgag ggaaatatta agtttttgga atgtaataag
      181 tatgttcaag agccgatttt gtctgcggat tttaatattt tgcccaatct ggcaatgcaa
      241 ataactccaa cttcaatagg aagtgctatt tataattttt cagatttttc tttaaaattt
      301 aatcttgcag gacaaattga aggtgaagaa ggtgttgcag taagtgacgg aacttatccg
      361 gttgtaaata taggcgggat agatgtagag ataacatgtg attttgttaa gaaaatatcc
      421 gtagtaatcg gtttgacttt gattattcct acgataataa ttgcttttgc tcttaataaa
      481 tttttagaac aggaaaaaac taaattatca gatttaaata ccaaaataca agaaagaact
      541 attgaaatta aaaaatattc tgatctttct tcgggtgcgg cttttgattt aaatgccgca
      601 attaattcca taacagtttc aaacagaact aaactttttt attatgaagc tgtgggaatg
      661 agtattccga gcaaattgtg gattaattat tatactacaa attctaccgg cggagtggat
      721 ataaaaggtc gctcaaatga cgtcggaagc atttatacct tttataaagg aataaaacaa
      781 cttgtgaata attcaaatgt aaggctttat aagcttgagc ttgcttctga ttctgtcgat
      841 gaccttgtat ccgattcttc atccggtcca aaattttatg attttgaaat aactaatatg
      901 actgaggcag aacttaatcc acaggctgca acttcgacac aaaatcaagc tgccggtcaa
      961 aatcaaacaa atcagcaaaa acctttgttt gatttcagca agccgttgtt tggttcaaaa
     1021 aatgattcca atcagactca aaataatcct caaagtgcaa cacctgcacc tgtaccaacg
     1081 ccgcctgccg ctccgcctgc ttcaccgccg gcagataagc tgccgcaaaa tttacaaaaa
     1141 atagacaagt tctagggaga aaaagtgcaa agatataaag atgctgtttt atatattgtt
     1201 tttatgatta tggcttttgc atatgtcata tttaagatgc agcctactgt tgttgaactt
     1261 atttctgtag aaaaagaaat ttcagccaag acaacggaac tggcagatct tgaaaggaaa
     1321 cttgaaacac ttaaagcagg gtctatggaa aaacaggtta agattgccgg tcaagctaaa
     1381 aagatattta aacccgaaat atccggactt gatgtcgaag gggcttccac agtgcttttt
     1441 gacgatataa ttgaaatggc gaagtataac ggtataaaat tttattctgt agaatatgtt
     1501 tataatccgc ccgaagatga atttgttaaa ggggctgcag ataagtataa tgtctgtcag
     1561 gtaagtatgc aaatggttgc agattattct gatttatcaa gttttttgaa agatatttat
     1621 aaatatccat atttgttgaa tatcgataaa atcgagcttg taccttatcc gaaaaacaaa
     1681 aaaattctta tgataaacat gcagcttaga ttatatacag aaaaataagt ttatttttgg
     1741 ttgcatatat agcagaagtt tgtttctaaa ggcatttttt tgctgtaatc agaaaaactg
     1801 tttcccattc tgcccctgtg gtcgttggtt aaaaaaggac aaacatatac tcctttttcg
     1861 gttaaaattc tgctgttttt gcagataata tcttctgaaa cattttctac aactccgtgc
     1921 tgcggagaat atttgttata ataattattt atttcaaaat tacctaaaac ggtgtcaaat
     1981 cctatttttt cgcaaacctt tttgaactca tgcaaaagag tcttttcatc ttctctgtag
     2041 taatttacta tgcttaaaat ggggttaaaa tcatatttaa taaggctttt tatagcatta
     2101 accgtttgtc tgtaagagcc tcttcctctt atatcatcgt ttttaatttc gttgtaatgg
     2161 tcaatactta atttaaaaaa tatctcattt cccccttctt cttcaacttt ttttaaaaac
     2221 cttgcttttt tctcgttaat aaaacttgca tttgtacaaa cacaaacatc cgcccgtttt
     2281 aggcaaattc ttaaaatggc attaaattcc ggatgagtca tgggctctgc accggtaaga
     2341 taaatacatt ccaaattttg ttcttttgtg tccgctattg cctgttttat tttatcaacc
     2401 gatataaagt cttttacatt tcttgaaaca ggaaaatcta tataacaatg tttgcaacgt
     2461 tgattgcagc tttttgcact aagctctata taaaggtttt ttaactcttc catttttgaa
     2521 acaggtgctg taattatgct catgtaaccc cctcatttgt gtaataaagg gctggattaa
     2581 tttttttaaa ttaatccagc cctattctta atcttatttt attaattaaa actacaaaat
     2641 atattcgaca acagggtata aaaaataatc agaagattaa aattttaatt gtttaattta
     2701 ttaataattc cttgaataac tattttgaca tgggcgtaat taagcccgcc ttgcatgtat
     2761 gcagcgtagg ggggtctgat aggaccgtct gcggataatt ctatggtcga gccttcaata
     2821 aaagttccac ccgccataat cacgctgtcc tcgtagcccg gtatgtaatc ggggatagga
     2881 gtcacataag aattaacagg agacaattcc tgaattgttt tgcaaaaatc ttccagaggc
     2941 ttttgggcat tgaatgttat ggtttggatt atgtccgtcc ttttttcatc aaatttcggg
     3001 gttgaaataa atcccatttc ctcaaaaact ttagcggcta agactgcacc tttcaaggct
     3061 tccgatacaa tagaaggtgc cataaaaaac ccttgaaaaa tcaatctgga ctggttaagc
     3121 atagcaccgc cttctgagcc gattccggga gctgtcagtc tcattgcggc ttgttcgaca
     3181 aattcttctt ttcctgcgat gtatccgcct gtttctacta ttccaccgcc ggggtttttt
     3241 atcaaagagc ctgcgattaa gtcagcaccg acttcaagcg gctctttttt ctcgcaaaat
     3301 tcgccgtaac agttatccac aaaacaaatg caattcggat ttttgctttt aactatatga
     3361 attatttttt ctattgttga aatatcaagc gattttctaa gcgaataacc tctggaacgt
     3421 tggattaaaa ccattttaca cattttatcg attgtttttt cgagaagttc aaaatcaact
     3481 tccatgtcgt tttttagcgg gatttcttcg taggtaaccc catgcccgat taaggaagct
     3541 cttttatcgc cacgcgttcc gatgacttct tccatcgtat cgtaaggtga acccgcaacg
     3601 gatataagtt tttccccatg cctgagattt ccgaaaagac agcaggcaag tgcgtgtgtg
     3661 cccgatacaa aatgatttcg cacaacggct ttttgggctt taaatgcatg ggcaaaaact
     3721 ttgtctaaag tttctctgcc gatgtcgtca tgcccgtagc cggagactgt cataaaatgc
     3781 tcggctgcga ctttgttttc gacaaaggca tcaaggactt ttttttggtt aaaatctctt
     3841 atttcatcga ttatttcaaa ctgtttttgt aattgcaatt cagtattttt aattatttga
     3901 gtgctgttca taattttatt ttattttaat cgggtttaat tttcaagcat ttacccattc
     3961 ggggattggg aaaatttttt ctttgtttta aaaacagatt atgaggaatt ttattttaat
     4021 tttaatattt ttgtgcctga caggcatcgc atttgcgcag aattataatg tctatcagcc
     4081 cggtgatttt gataaaaata aactatacga tgcggatgaa aaaatccttt ttatacttga
     4141 tttttcaggg agtatggatg aatatttagg caaagaaaaa aaagtgacct tgatgctaaa
     4201 aaccatgtca cagattctgc cctttataga caagaaaaag caagtcggac tgagagtcta
     4261 tggcaacaga cagggattta cccagtatga tgcctgcaaa gcaagctcgc ttcttgttcc
     4321 gataactcct gcaagtgccg ccgaaattga aatgaagctt gcaaaaaccc acccaaaagg
     4381 catgactccg attacttatt ccttgaacaa agcggtttat tcggattttt taggttttag
     4441 cggtcaaaaa cacatcattt tgctgactga cggaggtgaa aactgcgatg aaagcccatg
     4501 tctgtgggct atggagttaa taaaaacaag aagcgatgta aaaatcgatg taatcgcttt
     4561 taatatagac gatcaggatg atttggacca gctaaaatgc gctgcgcttg tcacgacagg
     4621 aaaattttat tctgcaaaaa ccgcagcaga gcttgttgac agcttaagaa aatctttaag
     4681 catacgaaaa gacgtcgaag caaagattat tttgaataag tgaaagatta aaatatcatt
     4741 cttaacgctt ttacaacatc tgcaggtcct gaagttgttt taattgggac tacatcaagc
     4801 tcttttgcac caataatttt tacttgatta ttaacccaat caaaaattga agacgctgag
     4861 tgtctacatt taaaaaacct attggcatca gcctctactt tttctaatac aggaaattgt
     4921 tttattaaaa cttctttgca acaactaata atgtaaggga tatctattgt acactcattt
     4981 agtttcccta ttattttttc agcacactgc attgctggct ctatgccatc atatgttaaa
     5041 gatcttccga caacaagttc tatagcatta atcgtcggta tttttgttcc aaaacttggt
     5101 gaattaactc tgttaatttg cattttattt aattcctttc cttttctaac acgttacaaa
     5161 tttataaagc acactcaata aaaaaattga cataaaaaat tctattctat tctattctat
     5221 tctattctat tctattgggc attatgattg gtttttagcg atttaaaact catgtttaca
     5281 atttgtaaca cttgaaaaag tgtatatttt gtggtacact ataattatga aagagaaaat
     5341 aattaaaaaa tatcttaaag aaaatggtaa aattccattt gatgattggt attacaaact
     5401 ggataaacct cttagaaata caattgctat aaggcttaca agagtattat caaatttgta
     5461 cggaaagcat aggaatttac caaacggtat aatagaattg aaatttgata atggcttaag
     5521 gctttatttt acagaaataa ataatgaaat attacttttg ttgcttggtg gaaacaaaca
     5581 aagacagtca gacgatataa ataaagctca agaatacttg aaagattatc aagaaaggac
     5641 aaaaaaacat gaccgcaaaa aataaaaaat taatagatac atttccgact tttgatattt
     5701 cacaagagtt gattgatgat gaaacggcgt tagattattt acaacaaatt ttgactcttt
     5761 atgttgaaga tggacgcgaa gacgtattta tgaattgtct aaaacctttg ataaaagcac
     5821 aaggttcagt ttctgatttt gcaaaaaaga caggaataag caggacttat ttttataaat
     5881 tgttcaacaa caaaataacg cccgaattgc cgacattaat tagaataata agcaatcttg
     5941 gttacaaaat gtcttttaat cttgaaaaat atgcttaata cttttcgata atttctttaa
     6001 tgcctttttt ggcaattttt aaaacttcaa gcatttgttt ttcgctgaaa ggatgacctt
     6061 cggcggtggt ttgaaactcg attattttgc cgctttttgt catgacaaga ttagcgtcag
     6121 cctgagccga agaatcttct tcgtaattca aatccagcct tatttcacct tctacaattc
     6181 ccgcagaaat agcgcccaaa ggttcaataa tagggttttg ctcaagtttt ttatctttaa
     6241 ttaatttttt aacggactcc tgaagcgcaa gaaacccgcc gcaaatgctt gctgttcttg
     6301 tcccgccgtc tgcctgaata acatcagcat caacaattat agtaatatct ggcatttttt
     6361 ccaaatcaac gcatgccctc aggcttcttc ctataagcct ttgaatttcc tgagttctgc
     6421 ccgaaacgct tgttctttct cttttgcatc ttgtgcttgt tgatgagggc aaaagcgagt
     6481 attcggcagt tacccagccg ctttttgcgt ctcttggcat ccacctgggt ttttcaaatt
     6541 caaccgaagc tgtcgttatt acttttgtat cgccaaattc cactaacaca gagccaagcg
     6601 cgtgttttgt gtaattcttt gtaaatttaa ttttccttaa ttcgtcattt tttctgtttt
     6661 ttctcatttc tttatcctta ttttggcgga atagcaattc cgccctacta ctttatttcc
     6721 tatttactat tcacctctcc gtaatcccac atataaatct gaccgcaata tctgcacacg
     6781 gcatgttcgt tcataaacat caaatcgggt atgaatgtgt agccgtcaac tgttatttct
     6841 atttcatttt tcttgttata tttttgggaa aacttttttt cgcccttgta atcgggtgaa
     6901 aacatcaatt caaatttatc gatcgatttg cagtttttgc ataaaaacat tttttaacct
     6961 ttttgaaaca taaatacatc tgtaatttct ttttacagtt caccgttcac agctcaccgc
     7021 tcaaccttca gccctgtcaa gaattttctt atccagcttt gcttcacctt tattgagtgc
     7081 cttataccaa agagcccaac atatgcttct gagaggaagt gtaaggcaga taaccgcata
     7141 ccccaaaaaa gccgaaacca caaactgagc aatcaaaaga gaagttattt gatatgcagt
     7201 cggtgactgt gtaagaattt tatttatttc atcgatagga aattggcttg cccaaccgtc
     7261 aagaggaatt gcaagaaaag caattatttt tgcggcatca aacaaaaatt taactgtttc
     7321 gggcaaaacc cagtatgtaa aaatccacaa aagtgacatc aatccgcaag ttttccaaaa
     7381 atttcctttt ataatatgaa aacttttttt aaagcatccg acgggagatt tgtcaggttc
     7441 aaacgtaaat acttggaaca caaggacaaa atacacaaat aaaatccctg ccacaagcca
     7501 aaataaagga aacatactta taatcgtgaa cagagcaaac aaaagccaaa gcccgacaaa
     7561 ggcaggtgtt cttcttgtta taacttcatt gtgtgccgca aaatcataga cttttcctga
     7621 tttaacaagg ttttctacca tggaatttat cgccccgtaa gatacaagat aatcccaaaa
     7681 agctttcata aatataaaaa gtcccggcaa agttatcagt attaaaacaa gaaaaattat
     7741 tgaaaaatta ttgaaaattc caccctgcgc gattaaattg gaaacattca gagtatagta
     7801 gtaggaagaa agcagggcta taattatacc caaaatttgt ccaaatacag gaaatgccat
     7861 atacttgaaa aatttagtaa aattagtaaa ataaagcttt gtcgcttgta taaaaattcc
     7921 aaaaacactg tttttaatcc gagccattgt ctagttcctt tttttgttat atgttaaagt
     7981 gtattacaaa ttgtcttatt atacaatact ttagaaaaac aaatcatgac tgaatcttac
     8041 aaagacttaa ttaaatcttt taccaaacaa gattatgaaa accgcgttaa tctgcacata
     8101 cactcaacct tctccgacgg gggggtaaat gctgtagaat tggttgaaaa ggcaaaagct
     8161 aaaggtttaa aatatatctc aataactgac cacaatactc tcaatgctta tttacaaaca
     8221 gctattttaa atgaagaaat tataatcccc ggaattgaat ttgactgctg gtttggaacg
     8281 gtttttttgc acatgctcgg ctacggagtt gatgtgaaca acaaaaaact tcaatcactt
     8341 tgcgccaaga caaaaagagg cactgaagcc gattgggtaa gaattttttc tttcaaacat
     8401 cccaaaagag ttattgatgc aatccacgaa gcaggcggaa tagcagtcct tgcgcatccg
     8461 gcatgctgtt ttactttcag ccttgaaaaa ttggtcaaaa atcttatgaa attcgggctc
     8521 gacgggatag aggtttatta cccttacgaa aggcacagaa aaatagttaa attccatacg
     8581 gttaaaaccg tggaaaaaat tgccgataaa ttagggctta ttaaaaccgg cggaacagac
     8641 gaacacggaa gcctgtgagt aaatagaaaa tagtaaatag tgaatagggt taaaatctat
     8701 ttaccattta ccatttactt aaaaagtctt ttagttttta gtatctttcc agatatttat
     8761 ttatttccca gcctgaaacg taagttctga aagaatccca ttcttttttc ttagagctca
     8821 taaattcatg gaagatgtgt tctcccaacg ctgctcttgc aacaagtgat ttatcaagct
     8881 gttccaatga ttctgcaagg cttccgggaa gtgaatcaat tctttgtttt ttgcgctctt
     8941 ttgaagtcag atgataaata ttgctttcaa ccggtgcggg cgggtcaatt ttgtttctga
     9001 taccgtctaa gcatgattcc aaaattgccg caaaggcaag gtaagggttg cagcttggat
     9061 caggtgagcg aagttcaact cttgttgcgt ttccgcgttt tgccggaact cttaaaagcg
     9121 cgcttctgtt agccaaagac catgccagat aaaccggtgc ttcatagccc ggaacaagtc
     9181 ttttatagct gttaactgtc ggatttgtaa ttgcggtgat gctttttaca tgtttcaaaa
     9241 gcccgccgat agcaaaaagt gccgcatccg aaagctgata ttcttttttc tcatcaagaa
     9301 aagcgttttt gccgtttttg aaaagcgaaa tgttgcagtg catacccgag ccgttaattc
     9361 caaaaatcgg ttttggcata aatgttgcat ggagtccgtg ttgagcggca atcgctttaa
     9421 ccgcaattct gaaggttaca acattgtctg ctgttgtcaa aatatcggcg tatttaaaat
     9481 caatttcgtg ttgaccgtca gccacttcgt ggtgagccgc ttcaatgtcg aaattcattt
     9541 tctgaagcgc agaaacgata tccgcacgga cattttcgcc taaatcttca ggtccgacat
     9601 cgtagtatcc tgctttatcc tgtgtttcgg tggtgatttt tccgtctttt ttctcgaaaa
     9661 ggaaaaattc cgcttcaggt cctaaattca ttgaaaatcc gagtttttcg gcttctttca
     9721 taactctttt taaattacag cggggacatc cttcaaatgg tgttccgtcg ggattgtaaa
     9781 tgtcgcagat aagccttgcg cttttggcgt tatcgtttgt tgtccagggc aaaatctgaa
     9841 atgaattttt gtccggatag aaaaacatat ctgatgtttc tatgcttctg aatcctttta
     9901 ttgatgagcc gtcaagcata atctcgtttg caagaacttt ttctatgtgt tcggatggca
     9961 cagccatgtt ttttacctga ccgtttatgt caacaaattg cagttgaata aatctgatat
    10021 tattttcttt tatcagtttt ttgatttctt cttcgctaaa tgttttgttg tttaaggcaa
    10081 cgtgtgtaaa atcgctcata ctatctcctt ttcatgtgat ttggtatatt tattatttaa
    10141 ttagaatacg ttttttgata aaggtcaagg gtttgtaaat tgaggatgcg gaaaaattaa
    10201 aaatttttct cgcacctcca tagtccagta aaagccaagc caccctctta ccaag
//
LOCUS       DAOQAU010000057        10199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_7669_length_10199_cov_6.551558, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000057 DAOQAU010000000
VERSION     DAOQAU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7669_length_10199_cov_6.551558"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..967
                     /locus_tag="PKI94_07545"
     CDS             77..967
                     /locus_tag="PKI94_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNW26624.1"
                     /translation="MSILVTGGAGFIGSHVTDFLIKNGYDVVIVDNLSSGKNRNINSK
                     AKFYNLDIISDKLEKIFKENNIEYVFHFAAQASVSSSIKSPVNDAKENILGTVNLLNL
                     CKKYKIKKIIYASTAAVYGIPEYLPVDENHSTNCLSFYGLSKLTAEKYIQLFGLDYII
                     FRFANVYGARQSCEGEAGVVAIFADKMKNNQKIQIHGDGTQTRDFVHVTDIAQICFEA
                     LNPKIKNEIINVSTNKAVSVNKLFELMAQKYGYSILPEHTQERQGDIKHSVLDNKKCL
                     KTFNIIPKTTLQKGLSSLII"
     gene            complement(960..1754)
                     /locus_tag="PKI94_07550"
     CDS             complement(960..1754)
                     /locus_tag="PKI94_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26625.1"
                     /translation="MKIAILYIGTGHYTIFWDEFFKSCEKYFIKDAQKHYFFFTDSKN
                     FKSDEKITIVPQENLGWPLITCLRYKILNKIKEDLKDYDYAFFFNGNMEFIRKITKEE
                     FLPIEKDGYIVASLHSGNKRVKFSKEFPYERNPLSTSYIPYGVGEKYFHAGILGGRTK
                     EFLELLDTCEKMMDEDLSNNIIPKFHDESVFNKYILNRPYKILSNYYIHPVHGKPFVW
                     LNQKVKIIQRDKSRFKYGGHAYLRGETNKKITPVSYFKEKIKQIFK"
     gene            complement(1779..2888)
                     /locus_tag="PKI94_07555"
     CDS             complement(1779..2888)
                     /locus_tag="PKI94_07555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26626.1"
                     /translation="MPTNNPKISILTPSYNHEKYVGSFIKSVLAQTEQDFELIIVDDN
                     STDNNVKEIQKFNDERIILIQHKYNKGINAGLNSAFEKSRGKYIVFVASDDMLEENHL
                     EETTKYLDKHPEVNLYYCSLSLIDEQGSHISGREKHYILNYNDKYEVLKDLFLKRNIL
                     FSPGMALRREAFEKIYPLNLSVIQYQDYIMNVELLIENEAHFSEKQLIKYRQSSQPTN
                     ISAQTTTVLSREVLEEEKVMNAFLKIKDIQTLKKIFGNDLKEFGEPVNETIPYFFGRM
                     AFKSSKNQKRRWGYKIIMDFIADAENLELLHKLYGFDFKQYLALAQNLEGMSSGLIAD
                     FDFKISRYKKLFNKFLVIAIILGVIVLGLLFGYFI"
     gene            complement(2875..3963)
                     /locus_tag="PKI94_07560"
     CDS             complement(2875..3963)
                     /locus_tag="PKI94_07560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNW26627.1"
                     /translation="MIKFLDLHKINEKYRQKIDAGIKRVLDSGWYLLGKEGEIFEKNF
                     AKYCGVKHAIGVANGLDALNLIIKAYGFGKGDEIIVPANTYIASILSISQNGCTPVLV
                     EPDIKTYNINPDLIEEKITSKTKAIMPVHLYGQAVEMEKIWVLAKKYDLKIIEDSAQA
                     HGAIYQGKRTGNLGDASGFSFYPGKNLGCLGDGGAVTTSDDELATKIRALRNYGSHVK
                     YQNIYKGTNSRLDEIQAAVLDAKLEGLDEDNQIRRDIANYYIQNIKNPLVVLPEPVTQ
                     EGHVWHIFALRVKNREAFQNYLTKNEIQTLIHYPIAPHKQEAYKEWNNLSFPITEQIH
                     REIISLPISPVMEREEFQRVIEVVNAYK"
     gene            complement(3956..4900)
                     /locus_tag="PKI94_07565"
     CDS             complement(3956..4900)
                     /locus_tag="PKI94_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HNW26628.1"
                     /translation="MILKRCWGIFKLLINKQQPVAIIMVDGGLGSQLNKLIIGEILKK
                     KTGALVKYDLSWFEDCGKSIDNKENRLFELTKVFKNIDFPIATEEEIKLYKKCFRHKN
                     KNSFKYNKKDLSKKPPVYLDGYFGNWQYLAEFQILGLQKPEFNLELSTKNQEILNRIS
                     NSEISIAVHIRRGDYVNSIHEVLNANYFINAINYMKNSFDKPVKFFIFSNGMDWVKNE
                     IIPNLNNADFELIEENNNDSGAYDFYLLTQCKHQIISNASFGFLGAFLNKNENKKVIT
                     PQVWIKKQKSDKLDFENSKAFHYKDWIVMSNEGEIIND"
     gene            complement(4900..5355)
                     /locus_tag="PKI94_07570"
     CDS             complement(4900..5355)
                     /locus_tag="PKI94_07570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNW26629.1"
                     /translation="MTNFIHKLSDVQSKNIGKNTNIWQFCVVLEKAVIGDNCNICSHC
                     FIENDVKVGNNVTVKCNVQLWDGIELEDNVFVGSNVTFCNDKKPRSKKRPEKFIKTVV
                     KKGASIGAGSTILPGITIGENAMIGAGSVVTKDVKPNITVMGNPAKEKK"
     gene            complement(5352..5924)
                     /locus_tag="PKI94_07575"
     CDS             complement(5352..5924)
                     /locus_tag="PKI94_07575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNW26630.1"
                     /translation="MNFSKFFKKFLKVKVCFFKKFLSDNFLKVYPKLNQPCLFLGKGE
                     IIIDKSAHLGYFPSPLFYSTYCHLEARNPSSKIIIGKDVFINNNACIISNGAKIEIGQ
                     NCRIGMNFQCFDSDFHGLKAENRDNSEAIINKDTTIGNNVFIGNNVIILKGVTVGEGC
                     VIAAGAVVTKSFPEKSIIAGNPAIAKGEVK"
     gene            complement(6009..6410)
                     /locus_tag="PKI94_07580"
     CDS             complement(6009..6410)
                     /locus_tag="PKI94_07580"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNW26631.1"
                     /translation="MNYKLIDFVKRGDKRGSLVAIEGNQDIPFEIKRVFYIFDTQGQD
                     VIRGDHANRKSKFVLVMVSGSCKIKVFSDKEKFEIIELNSPDKGLFLDTLTWKEMYDF
                     SPNSVMLVLASEHFDADEYISSHQELLDLIN"
     gene            complement(6556..9771)
                     /locus_tag="PKI94_07585"
     CDS             complement(6556..9771)
                     /locus_tag="PKI94_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26632.1"
                     /translation="MAGDTLNFTSNINATSSIGSLGSFTLTLNGNNHTINGSSSGFSL
                     SSSAANYRFNSINLNNFLRTTGGAIYNSMGTITVNGCSFTGNRATSGTTGGGAIYNTT
                     TSTNNITNSTFNSNTATTSGGAIFANGNTEITGGSFNNNTAGTSGGAIFNNVGDSSHN
                     NKHLYLTNTDFTGNSSVNGGALYNSPYEYTTGIATRIIGGTFTDNTATASGGGIYNST
                     GQTTITGTTFITNTASLSGGAIYNTSGSPFSIDNCTFTGNSSARGGAFYDTAGSTATV
                     TNSDFNSNVATVSGGAIYANGPITVSGSSFTDNQATTSGGAIYNTGTATITDTSFSGN
                     SATNGSGGAIYNETGYSLSLTDTDFSNNTASDSGGAIYNNGTTTINNGTLSNNSAVFG
                     GGIYNSAGSTLNSTNTNFSNNTATGNGGAIYNAGTAYLKADGATSLFSGNNTDIYNTG
                     TIYLNAGNSGVITFNDAIASDSNTRNIYINSAAGSATLTNGTINFNNAVTNSSLNIAN
                     GIVNFGENISLTNDKLTFSGGTSNFINQNLNTSNDITLNGGTINLNSGNGGSSYIQDK
                     IVGSSSGTININKAVGSNPTNGTVNFNNAVTGGVNVNLYNGTMALSKDSYINGNNLGI
                     FGGTLNTQNSIIGTMALDKVNFGGIGNWLIDVNLANAIGDKITSTNPVIGAGSLNISG
                     VNLLNDSNSLITNIYVADANMKNYLTTSVTEALGTLFKYKVSYDGVAGALNFLQNGVN
                     PAVVTGDVSQTQTFLLQTAIDRQFFGNIDAFMSFPLAQRESTICCALSPYNKQYTGAA
                     CPITGNGTFSPIYSCDLNRGIWAKTFASFESIPLRNGPVVSTIEYGTLIGADSILKYL
                     GRGFVGNTSVYVGYFGSNQNYDGVGVSQNGALVGIAENIVKGNFFLTAMASVGSSLGN
                     SNTRWGTDNFSSLFAGLGAKGGYNFEFKNGEYIIQPNLMLAYTFTNTADYTAASGVNI
                     STKPLNAIQVAPGVRLIKNLKNEKGQIYLIAAYVQNIMDKTNFIANDLQLPQLSIDPY
                     FEYGVGYQRVWKERFTGFFQTLFRGGGRNGVAFQFGFRWAI"
BASE COUNT         3119 a         1855 c         1584 g         3641 t
ORIGIN      
        1 tttataaaat tcttccaaaa ttgacattgc ctcctttttc gcttaatatt ttcttatatt
       61 aaagaggaat tttattttga gtattttagt cacaggcggt gcaggtttta taggctctca
      121 cgtaaccgat tttcttatta aaaacggtta tgatgtagtt attgttgata atttgtcctc
      181 aggtaagaac agaaacatca attccaaagc gaaattttac aatcttgata ttatctcgga
      241 taagcttgaa aaaatattca aagaaaataa tattgaatac gtttttcatt ttgcggcgca
      301 ggcaagcgta agttcttcaa taaagagtcc tgtcaacgat gctaaagaaa atattttagg
      361 aacagttaat cttctgaatc tttgcaaaaa atataaaata aaaaaaataa tttatgcttc
      421 taccgcagct gtttatggaa tccctgaata tttgccggtt gatgaaaatc attctacaaa
      481 ttgtttatca ttttacgggc tttcaaaact taccgccgaa aaatatattc agcttttcgg
      541 acttgattat ataattttca ggtttgcaaa cgtttatggc gcaaggcagt cttgcgaagg
      601 cgaagccggt gtcgttgcaa tctttgccga taaaatgaag aataatcaaa aaattcaaat
      661 ccacggtgac ggaactcaga caagagattt tgttcatgtt acagatatag cgcaaatttg
      721 ttttgaagca ttaaatccga aaattaaaaa cgaaattata aatgtttcta caaacaaagc
      781 ggtctcggtt aataaacttt ttgagcttat ggctcaaaaa tacggttatt ctattttacc
      841 cgagcacacc caagaaagac aaggcgatat aaaacacagc gtccttgata ataaaaaatg
      901 cttaaagact tttaacataa ttccaaaaac cacgctgcaa aaagggctaa gctctttaat
      961 tatttaaata tctgcttaat tttttcttta aaatagctta cgggggttat ttttttattt
     1021 gtttcacccc ttaaataagc gtgtccgccg tatttaaatc ttgatttgtc cctttgaata
     1081 attttaactt tttgatttag ccagacaaaa ggttttccgt gtacaggatg aatataatag
     1141 ttgcttaata ttttataagg acgatttaaa atatatttat taaaaacgct ttcgtcgtgg
     1201 aatttcggga taatgttgtt gcttaaatct tcatccatca ttttttcaca ggtgtcaagc
     1261 aactctaaaa attcttttgt cctgccgcct aaaatgccgg catgaaaata tttttcaccc
     1321 accccgtagg gtatataaga agttgacaaa ggatttcttt cataaggaaa ttccttgctg
     1381 aatttgactc ttttgttgcc tgaatgaagt gatgcaacaa tatatccgtc tttttctatt
     1441 ggtaaaaatt cttctttagt tatttttctt ataaattcca tattcccgtt gaagaaaaat
     1501 gcgtaatcat aatctttgag atcttctttt attttattta aaattttata tctaagacat
     1561 gttatcaaag gccagcccag attttcctga gggactatgg ttattttttc atcagattta
     1621 aaatttttac tgtcggtaaa aaagaaataa tgcttttgcg catctttaat aaaatatttt
     1681 tcgcatgatt taaaaaattc atcccaaaaa atagtatagt gccccgttcc tatgtacaaa
     1741 atagctatct tcatatagac ctttcttgtt tcagattatt atataaaata gccaaaaagt
     1801 aaacccaaga caattacccc taagattatt gcaataacta aaaatttatt aaataatttt
     1861 ttatatctgc ttattttaaa atcaaagtca gcaattaagc cgctcgacat tccttctaaa
     1921 ttctgggcaa gggcaagata ttgcttaaaa tcaaacccat acagtttatg caaaagttct
     1981 aaattttcag cgtcagcgat aaaatccatt attattttat aaccccatct gcgtttttga
     2041 tttttagaag atttaaacgc cattcttccg aaaaaataag gaatagtttc attcacaggc
     2101 tcaccaaact cttttaagtc attaccgaag atttttttaa gcgtttgtat gtcttttatt
     2161 tttaaaaaag cgttcattac tttttcttct tccaaaactt cgcggctgag aactgttgta
     2221 gtctgcgcac ttatgtttgt cggctgggaa gactgtctgt attttataag ctgtttttca
     2281 ctaaaatgtg cttcattttc aattaaaagc tcaacattca ttatgtaatc ctgatattga
     2341 attacagaca aatttaatgg ataaattttc tcaaaagctt ctcttcttaa ggccattccg
     2401 ggtgaaaaaa gaatattgcg ttttaaaaat aaatctttaa ggacttcgta tttgtcgtta
     2461 taatttaata tataatgttt ttctcttcct gatatatggc ttccttgttc gtcaattaaa
     2521 gataaagagc aataatataa atttacttca ggatgtttgt ccaaatattt tgttgtttct
     2581 tcaaggtgat tttcttcgag catatcatca gatgcaacaa aaacaatata tttacccctt
     2641 gatttttcaa aagcagagtt aagtcctgcg tttatgcctt tgttgtactt atgctgaatt
     2701 aagattatcc gctcgtcgtt aaatttttga atttccttca cattattgtc tgtggaattg
     2761 tcatcaacaa ttatcagttc aaaatcctgt tctgtttgtg caagaacact ttttataaaa
     2821 cttcctacat atttttcatg gttatacgaa ggagtcagaa tggagatttt aggattattt
     2881 gtaggcattt actacctcaa tgactctttg aaattcttcg cgttccataa caggcgaaat
     2941 cggcagagaa ataatttctc tatgaatttg ttctgtaatc ggaaacgaca aattattcca
     3001 ctctttgtaa gcttcttgct tgtgaggagc tatcgggtaa tggattaaag tttgtatttc
     3061 attttttgtc aaataatttt gaaaagcttc tctgtttttc acccttaagg caaaaatgtg
     3121 ccatacatga ccttcttgtg ttacaggctc aggcaaaaca acaagaggat tttttatatt
     3181 ttgaatgtaa taatttgcta tgtcacgtct tatttgatta tcttcatcaa gtccttccaa
     3241 cttagcatct aaaacagcgg cttgaatttc atcaagtctt gaatttgtac ctttatatat
     3301 gttttgatat ttaacgtgcg agccgtaatt tctcaatgcg cgaattttag tcgcaagttc
     3361 atcgtcactt gtggttaccg ctccgccgtc accaagacag ccgaggtttt tacccggata
     3421 aaaactgaaa ccgcttgcat cacccaaatt ccccgtcctt ttgccctgat aaatcgcacc
     3481 gtgagcttga gctgaatctt ctataatttt taagtcatat ttttttgcca atacccaaat
     3541 tttttccatt tcaacggctt gtccataaag gtgtaccggc atgatagctt ttgtttttga
     3601 agtaattttt tcttctatta aatcagggtt aatgttgtat gtttttatat ccggttcaac
     3661 taaaacagga gtacagccgt tttgagaaat tgaaagaatt gaagctatat aggtgtttgc
     3721 aggaacgata atttcatctc ctttaccgaa tccgtaagcc tttataatta aattcagcgc
     3781 gtcaagcccg tttgcaactc cgattgcgtg ttttaccccg caatatttgg cgaaattttt
     3841 ttcaaaaatt tcgccttctt ttccaagaag gtaccaaccg ctgtctaaaa ctctttttat
     3901 tcctgcatct attttttgtc tgtatttttc gtttatttta tgcaggtcta aaaatttaat
     3961 catttattat ttctccttca tttgacatta caatccaatc tttgtaatga aatgctttac
     4021 tgttttcaaa atcaagttta tcactttttt gtttttttat ccatacttga ggcgtaatta
     4081 cttttttatt ttcatttttg ttcaaaaacg cacctaaaaa cccgaatgag gcatttgaaa
     4141 ttatctgatg tttgcattgt gttaaaagat aaaaatcata agcacccgag tcattattat
     4201 tttcttctat cagttcaaaa tcagcattgt ttaagttcgg aataatttca ttttttaccc
     4261 agtccattcc attagaaaaa ataaaaaact ttacgggttt gtcaaatgaa tttttcatat
     4321 aatttatcgc attaataaag tagtttgcat ttaagacctc gtggattgag tttacataat
     4381 cgcctcgtct gatgtgtacg gcaatcgaaa tttccgaatt tgaaatccga ttaagtatct
     4441 cttgattttt tgtagataat tccaaattaa attccggttt ttgcagtcct aaaatttgaa
     4501 attcagccaa atactgccag tttccgaaat atccgtccaa atacactggg ggttttttgc
     4561 ttaaatcttt tttattatat ttaaaactgt ttttattttt gtggcgaaaa cattttttgt
     4621 aaagcttgat ttcttcttcg gttgcaatgg gaaaatcaat atttttaaaa acttttgtca
     4681 gttcaaaaag tctgttttct ttattatcaa tacttttgcc gcaatcttca aaccagctca
     4741 aatcgtattt aacgagtgca cctgttttct tttttaaaat ttctccgatg attaatttgt
     4801 ttaattgcga gcctaagccg ccatccacca ttataattgc aacgggctgt tgcttgttta
     4861 ttaaaagttt aaaaataccc caacatcttt ttaaaatcat tattttttct cttttgcagg
     4921 atttcccatt acggttatat ttggctttac atcttttgta acgacagaac ctgctcctat
     4981 catcgcattt tcacctattg taattccggg taaaatagtt gagccggcac ctattgatgc
     5041 accttttttt acaacagttt taataaattt ttcgggtctt ttttttgaac gaggtttttt
     5101 atcgttgcag aaagttacgt ttgaaccgac aaaaacgttg tcttcaagtt ctattccgtc
     5161 ccaaagctga acattgcatt tcaccgtaac attgtttccg acctttacgt cattttctat
     5221 aaagcaatgt gagcaaatat tgcaattatc tccgattact gctttttcta aaactacaca
     5281 aaattgccat atatttgtgt ttttgccgat gtttttgctt tgaacatcag aaagtttatg
     5341 tataaaattg gtcatttaac ttcgcctttc gctatggcag gatttcctgc gattattgat
     5401 ttttcaggaa atgattttgt gacaacagca cctgctgcta ttacacaacc ttctcctacg
     5461 gtaacacctt ttaaaattat tacgttgttt cctataaaaa cattattacc tattgtggtg
     5521 tctttattaa taattgcttc ggaattatcc ctgttttcgg ctttaagccc atggaaatcg
     5581 ctgtcaaaac attgaaaatt catgcctatt ctgcaatttt gccctatttc gatttttgcc
     5641 ccatttgaaa tgatgcacgc attgttgttt ataaaaacat cttttccaat aattattttt
     5701 gaacttggat ttctcgcttc aaggtggcag taagtactat aaaaaagcgg cgaagggaag
     5761 taccctaaat gtgcactttt gtcgattatt atctcgcctt ttcctaaaaa aaggcaaggc
     5821 tgatttaatt tcggataaac ttttaaaaag ttgtctgaga gaaatttttt aaaaaaacaa
     5881 actttgactt ttaaaaattt tttgaaaaat ttgctaaaat tcatttactt agagccaatc
     5941 ttttaaaata aaagcaaccc aattattata cataaaaaaa taaaactaaa caaattaaaa
     6001 aattaatctt aattaatcaa atctaaaagt tcctgatgcg aacttatata ttcatcggca
     6061 tcaaagtgct cgcttgcaag cactaacatt acagaattag gtgaaaaatc atacatttct
     6121 ttccaggtaa gtgtatctaa aaacaagccc ttatcaggtg aattaagttc aattatttca
     6181 aatttttctt tatctgaaaa cacttttatt ttgcaagagc ctgaaaccat aacaagtaca
     6241 aattttgatt tgcggtttgc atgatctccc cttataacat cctgcccctg cgtatcaaaa
     6301 atataaaaaa cgcgtttaat ttcaaaaggg atatcctgat tgccttctat tgcaaccaag
     6361 gagcctcttt tatcaccgcg tttaacaaaa tcaattaatt tgtagttcat tttttgattg
     6421 taatataaaa catcaaatag tgcaagaaat ggcaaaataa aaaatttatg acaataatag
     6481 caaattcaat atatataagc ctttaaatca cccacctgaa cccgaattgg gggtaaatgt
     6541 gatttaatcc tttcatcata tcgcccacct gaacccgaat tggaaggcaa ctccatttcg
     6601 ccctccgccc ctgaacaatg tttggaaaaa ccctgtgaat ctttctttcc aaactctttg
     6661 gtagccgact ccatattcaa agtaaggatc gattgaaagt tgaggtagct gtaaatcatt
     6721 ggcgataaaa tttgttttat ccattatatt ttgtacataa gccgctatta aatatatttg
     6781 tcctttctca ttttttaaat ttttgataag cctgacgcct ggtgcaacct gtattgcgtt
     6841 taaaggtttt gtacttatat taacgcctga tgctgcagta taatcagctg tattcgtaaa
     6901 agtgtacgcc aacattaaat ttggctgaat aatatattct ccgtttttaa actcaaaatt
     6961 ataaccccct tttgctccta aacctgcaaa taatgatgaa aaattgtctg tcccccacct
     7021 tgtatttgaa tttcctaagc ttgagcccac acttgccatg gcagtcaaaa agaaatttcc
     7081 tttaacaata ttttcagcta taccgaccaa agctccgttt tgtgagacac caaccccatc
     7141 ataattttga tttgaaccga aataacccac ataaacgctg gtattcccga caaaaccgcg
     7201 tcccaaatat tttaatatag agtcagcacc tattaatgtt ccatactcaa tagttgaaac
     7261 aacaggacca tttcttaaag gtatgctctc aaaactggca aaagtttttg cccaaatgcc
     7321 tctgttcaaa tcacacgaat aaatcggaga aaatgttccg tttccggtaa tcggacacgc
     7381 tgcaccggtg tattgtttat tataaggact tagggcacaa catattgttg attctctttg
     7441 cgctaaaggg aaggacataa atgcatctat gttcccaaaa aactgtctgt caatagcagt
     7501 ttgcaacaaa aaagtttggg tttgtgatac atcaccggta accacagcag gattaactcc
     7561 gttttgcaga aaattaagtg ctcccgctac cccgtcatat gaaaccttgt acttaaataa
     7621 tgtacccaaa gcttctgtaa cacttgttgt taaataattt ttcatgttag catctgccac
     7681 ataaatgttt gttatcaaag aatttgaatc atttaataaa ttaacgccgc ttatatttaa
     7741 agagcctgca cctataaccg gatttgttga agtaatttta tccccgattg catttgccaa
     7801 attgacatca attaaccaat taccgatgcc tccaaaatta actttatcaa gagccattgt
     7861 tcctattata ctgttttgag tatttaatgt ccctccaaag attcccagat tattgccgtt
     7921 gatgtaagaa tctttgctta atgccattgt tccgttgtaa agattaacat ttactccccc
     7981 tgtaaccgca ttgttaaaat taaccgtacc attggtagga ttgcttccta ctgctttatt
     8041 tatatttata gtgccggaag atgagccgac aattttatcc tgaatatagc ttgaacctcc
     8101 gtttcctgaa tttaggttta tagtcccgcc gttaagtgta atgtcatttg aagtatttaa
     8161 attttgattt ataaaatttg acgtacctcc ggaaaaagtt agtttatcat ttgtcagcga
     8221 aatattttct ccgaaattta caatcccgtt ggcaatattt aatgaagaat ttgtaaccgc
     8281 attgttaaaa ttaatcgtcc cgtttgtcaa tgttgctgat cctgccgctg aattgatata
     8341 aatgtttctg gtgtttgaat ctgaagctat tgcatcatta aatgttatta ctccgctgtt
     8401 tcctgcattc aaataaattg ttcccgtatt ataaatatcc gtattattcc ccgaaaataa
     8461 tgacgtagca ccatcagcct ttaaataagc tgtacctgcg ttataaatcg caccgccgtt
     8521 acccgttgca gtattatttg aaaaattagt gttggttgaa tttaaagttg aacctgcgct
     8581 attgtatatt ccgcccccaa atactgccga attgtttgat aaagttccgt tatttattgt
     8641 ggttgtaccg ttgttatata ttgctccacc gctatctgaa gccgtgttgt ttgagaaatc
     8701 ggtatcggtt aagcttaaac tgtatcctgt ttcattataa atagctccgc cgcttccgtt
     8761 tgttgccgag ttgccggaaa atgatgtgtc ggtaattgtt gcagttcctg tattataaat
     8821 agctccgccc gatgttgttg cctgattatc tgtaaaagaa cttcctgata cagttattgg
     8881 tccgtttgca taaatcgcac cgccgcttac agtggcaaca ttagaattaa aatcagaatt
     8941 tgtaaccgtg gctgttgatc ctgcggtatc ataaaatgca cctccccttg cggaagaatt
     9001 acctgtaaaa gtacaattat ctattgaaaa aggactgccg gaggtattat aaatagcacc
     9061 gcccgacaaa cttgccgtat ttgtaataaa tgttgtcccc gtgattgtgg tttgacccgt
     9121 gctgttataa ataccgccgc ctgaagcagt tgcagtattg tcggtaaaag tcccgccgat
     9181 tattctggtt gcaattcctg tggtatattc atacggactg ttgtaaagag ctcctccatt
     9241 aacagaagaa ttgcctgtaa aatcagtatt ggtaagatat aaatgcttat tgttatgact
     9301 ggaatctccg acattgttaa aaatcgcacc gccggatgtt cctgccgtgt tgttattaaa
     9361 agaaccgccg gttatttcag tatttccatt ggcaaaaata gctccgccgc ttgtagttgc
     9421 ggtgtttgag ttaaaagttg aatttgtaat attattcgtt gatgttgttg tattataaat
     9481 tgccccaccg ccggtagtgc ctgaagttgc tctattcccc gtaaaactac atccgtttac
     9541 ggttattgtt cccatgctgt tataaatggc accgccggtg gttctgagaa aattatttaa
     9601 atttattgaa ttaaaacggt aattggctgc agaagacgac aaagaaaaac cggaagaaga
     9661 cccatttatc gtatgattat taccatttaa ggttagcgta aaagaaccca agcttccgat
     9721 tgaggaagtt gcattaatat ttgatgtaaa attaagcgta tctcctgcca caaaagcata
     9781 tgcacctctg ttaataattg cattttgttg tggtgccccg ctaaagtgat ctatattata
     9841 tgtagttgca gcaaatgtct tgatagtatt ataattaagt agcaatatgc atgacaacca
     9901 tattaaaaat aagttgcctc tcttcatttt attaagttta ttatttaaat aaatttaata
     9961 attccctatc ggagcatcat taaagattta aataaaattt gctaaagtat aaatattttt
    10021 tttattaaaa taacaacaac aatatgaaaa aactgataaa aacgtttatt gttaactatt
    10081 ttgctcttgc aacatttttt cggcacgttc aaaatcctgc ggctcatcaa catcaaaatt
    10141 tatgtccgtt aaatatatgc aagggttttc gccgatgaaa tcttttactt ttttgaaaa
//
LOCUS       DAOQAU010000058         9862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_8053_length_9862_cov_6.984399, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000058 DAOQAU010000000
VERSION     DAOQAU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_9862_cov_6.984399"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKI94_07590"
     CDS             complement(<1..394)
                     /locus_tag="PKI94_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNW26633.1"
                     /translation="MGYKILSKKELCPNQYEITIEAPYVVRNAKAGQFIILRVDQDGE
                     RVPLTIADFDKEKGELTIVYMAVGYSTKKLAMLDVGDEIADLVGPLGQPTHIKKYGTV
                     VCLAGGYGAAPCYLIAKAFKEAGNKVYMI"
     gene            complement(410..1765)
                     /gene="accC"
                     /locus_tag="PKI94_07595"
     CDS             complement(410..1765)
                     /gene="accC"
                     /locus_tag="PKI94_07595"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNW26634.1"
                     /translation="MFKKVLIANRGEIAVRIIRACRELGIPTVAIHSEADANSLHVSL
                     ATEAYCIGPAQSKDSYLSIPAIISAAMMCGADAIHPGYGFMAERADFVDICNQHGIKF
                     IGPSADAMRKMGDKATARKTMKEHNVPITPGTDLVKTVDDARDFAKKVGYPIIIKATA
                     GGGGKGMRIVRKDDELEIMMNMCQNEAQNSFGNPGVYIEKYLENPRHIEVQILGDSFG
                     NVVHLGERDCSIQRRHQKLIEEAPSPAINEHTRKEMGEAAVRAAKAINYEGAGTIEFL
                     LDSDGKWYFMEMNTRIQVEHCVTEMISNIDIVREQIIVAAGEKLDFAQKDVLLRGHAI
                     ECRINAENPDKDFMPCPGKIEGYVTPGGFGVRVDSHAYQGYSIPPFYDSMIGKLICWG
                     RNRQEARRRMYRALKEYVITGIETTIPFHQDIVEDDVFISGDFNTGFIEEYYRRKNVA
                     E"
     gene            complement(1876..2316)
                     /gene="accB"
                     /locus_tag="PKI94_07600"
     CDS             complement(1876..2316)
                     /gene="accB"
                     /locus_tag="PKI94_07600"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNW26635.1"
                     /translation="MNFDMDYVKKLIKILEESSLTEIALEDGEKAIMLKKEKEVIAAP
                     QVVTATMTSSTAGLSESSVCVAAPKGKAITSPMVGTFYKAPSPGAQPFVEVGATISEG
                     QVVCIIEAMKLMNEIESEHAGKIVEICVEDGQAVEFGQVLMYVE"
     gene            complement(2398..2955)
                     /gene="efp"
                     /locus_tag="PKI94_07605"
     CDS             complement(2398..2955)
                     /gene="efp"
                     /locus_tag="PKI94_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNW26636.1"
                     /translation="MISVNDLKTGLTLDLDHGLWSVVEFLHVKPGKGAAFVRTKLKNV
                     ETGQVIERTFRAGEKVARAMLDRRDMQYLYKEGKSFVMMDMENYEQISLSEEQIGEGI
                     KYLKENMMLSVLLHDGKTIGVDMPMQVELEVVDTPPSEKGNTAQGGTKRATTETGAIV
                     NVPFFVQNGDRIRVDTRTNEYLDRC"
     gene            3180..4271
                     /gene="rsgA"
                     /locus_tag="PKI94_07610"
     CDS             3180..4271
                     /gene="rsgA"
                     /locus_tag="PKI94_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNW26637.1"
                     /translation="MSKLKGQVFKIHSDFYYVKFADNAKFFECKIREILKKQQEKVMV
                     GDFVECSDINPQKHTAVIENIYPRKNFLTRPSVANVEQVIIVSSIKEPDLNFEQLNRY
                     ICLAKHHKIKTKLCFNKNDLSEDDSVIEKIFEIYEPLGFEIIFTSALEKLGIEDFAQM
                     LKGKMTVFCGTSGVGKSSLINSIYPDLNLKTKPVSEKTGRGIHTTRHCEIIEIKRCED
                     AKMRRYGNIHASTYPRIHAPIKIIDTPGFSNLKFDFLLPTQIDELFDEIKQHKSDCKF
                     KDCLHIQEEGCNVLAHIDEIDLSRYQSYLAFVQEAKGFKEKIKTEGTKKESFHKKTLN
                     KKVAKISNKKRQSARNTLKQQLYKDLEHA"
     gene            4264..5139
                     /locus_tag="PKI94_07615"
     CDS             4264..5139
                     /locus_tag="PKI94_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNW26638.1"
                     /translation="MPDNMREYIELINKNLDKFLKITYPQEIFESMRYSVLAEGKRLR
                     PVMCLESCRIFGGKIEDAIPTACAIEMLHAQSLIHDDLPCMDNDDFRRGKPTNHKAFS
                     EATAVLAGDALLTLAPQIIIQQSENLDAQVKIKLVEQYCIAAGAFGLIAGQIVDIQSE
                     GQKISPKTLEFIHTHKTADLFKLALKAGAIIAKADEKIIKAMDDLGQCLGFAFQICDD
                     ILDETSSFEKMGKTLGKDKDAQKSTYTALFGVKKARSEVICLLNKAHDIISKNNIKSG
                     ILEEIIKGIQERSNS"
     gene            5141..5608
                     /locus_tag="PKI94_07620"
     CDS             5141..5608
                     /locus_tag="PKI94_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNW26639.1"
                     /translation="MINGTQIVNTGYEVMLTGIFAALIAQIIKFLLHLIVKKKIDFKV
                     FTTTGGMPSSHSAGVVGLSTAVGLISGFDSIIFAVSAGYATVVMYDAAGLRRASGKMA
                     TCLNKIMDDFYKHDVQSAGGKLKELLGHTPVEVFAGALLGFVYACYMHFYFLS"
     gene            5617..5895
                     /locus_tag="PKI94_07625"
     CDS             5617..5895
                     /locus_tag="PKI94_07625"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNW26640.1"
                     /translation="MKEEKIIITISGTDKIGIVATITAILAKHCVNIEDIKQTLMQGH
                     FVMFMLGDISASEHSFKEIKEALIQAGKEIQMEVWVQRKAIFDKMHTI"
     gene            complement(6295..9849)
                     /locus_tag="PKI94_07630"
     CDS             complement(6295..9849)
                     /locus_tag="PKI94_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNW26641.1"
                     /translation="MKPSKIILITVFSFCILLYLAFIYALPTFFDINDYLPQITQTIQ
                     EKTGIVIKFKDAKTFSDWKLSAGATAQRVDLSYPNGEKFGQINDFKIAVSVPYLIFGK
                     IKLTTLDSQKALFNLKVNSKGQFLLEKYLKPIQFEEAYGQKLPLRFSFYPSIPDIKIE
                     KYRVSLINTDTSKGYFFKGKNFQLSDFVLDKKVKLKAQGALFLDNRKQITYDICVFTK
                     VLPEYNFQKSKTVSDFNIVKLFEDLYRYKFLAQIKSDLKISGEKQDLKIDGNLDLENI
                     STAIDGVTLPNSKINLKFDGHKIKIDSNIFTSQSDNSTVEGTFENGDKKYLNLDVKSK
                     KTEISNLFLIANNLCRIFGIKKFDKIHADGKLSADFKLKSDFKTVHSAGYLKIENAKL
                     DYKLYNASLSSIFADIDFSQDMVNIKKASAILNSQPIAIKGSLDKNANADILIDGKNL
                     QLKGLLATLGKRDFLSQNDVLGGSVSVSTKLKGRLDNLLPEINTSFNNLIIYNKPHRA
                     KISVQSANANSTIKNKEFKGKINANSIKISSQNFFKLILLPELSVSFDKKDLNIEKSS
                     ILMDNSKLNFYGKISNYNSPNFFVDIKAKGFILAKDISSMTKNYKPESIGKLPIIIKL
                     YGGEKQKIQAQMLANKNNHVSFVDIKGLGQTSLVNCDLTLDKDIIIEEIALYSTKAIN
                     SLSENFKTNTSSGYKVLTLSGKISDINSEANFRDIGIKIPNQLTVSLPDYPNSTARLK
                     GDLTMNGNIKNPDIEGYLSISSLSIPELQLGFKNSMIHFNQNFISATCPEGLFGNSIV
                     NINALALNNFSQGLVINNVDITSENLELSDLTGLISKLPKKTDASFTVLSGTSRLDRF
                     KAADIITTNVTSNISLRNNIFSMDNVRGDAYLGKFAGSINYDLIWQKLGLNLQGRGLS
                     AAVAIRALSKMKEEIHGQLDFDSNITMSGQNRNQLLKTLKGNTKFVISNGEMGTLGKL
                     EHLLYAQNIVSNNLFKTNLNLIAKAVSAKNTGVYKYMRGDVDFSKAWANISSLTTTGP
                     SMSMYITGRYHLIDEVANLTILGRVSDDVAQVLGPLGDISVDKIISSITKTDLPQRQI
                     TTNANYEKIDLIPPLTPKTNLPTKIFKVIIDGKVQSQSSVKSFKWLAAPQALQYIENS
                     PAREDNFVQKNTVEQNQKENPKEVGIPDFVDSLPDLKR"
BASE COUNT         2977 a         1891 c         1719 g         3275 t
ORIGIN      
        1 taatcatata aactttgttc cctgcttctt taaaagcttt tgcaatcaaa tagcagggtg
       61 cagcaccgta gccgcctgca agacaaacga ctgtcccgta tttttttata tgagtgggct
      121 gtcctaaagg tccgaccaaa tcggctattt catcaccaac atcaagcatt gcaagttttt
      181 ttgtggaata gccgactgcc atataaacaa tcgtcaactc gcctttttcc ttgtcaaaat
      241 ctgctattgt aagcggtact ctttccccgt cttgatccac cctcaaaata atgaactgtc
      301 ctgcttttgc gtttcgcaca acatacggtg cttctattgt aatttcgtat tggttcggac
      361 aaagttcttt ttttgataaa attttatatc ccattttagt tataaatccc tattctgcaa
      421 cgtttttgcg tctgtaatat tcttcgataa atcctgtgtt aaaatcgccg gaaataaata
      481 catcgtcttc tactatatcc tgatgaaaag gaattgtcgt ttcaattccg gtgattacat
      541 attccttaag cgcacgatac attctacgcc ttgcctcctg cctatttctt ccccagcaga
      601 taagttttcc tatcatagaa tcgtaaaacg gcggaatcga atacccttga tatgcgtgcg
      661 aatccaccct tacgccaaag ccaccgggtg taacgtaacc ttcaattttt cccggacacg
      721 gcatgaaatc tttatcagga ttttccgcgt taattctgca ctctatagca tgtcctctta
      781 aaagaacgtc tttttgcgca aaatcaagct tttcacctgc tgccacaata atttgttctc
      841 ttacgatatc aatgtttgaa atcatctctg taacacagtg ttcaacctga attctggtat
      901 tcatttccat aaaataccat tttccgtcgc tatcgagcag aaattctatc gttcctgcac
      961 cttcatagtt tatagctttt gcggcacgga ctgccgcttc gcccatttct tttcttgtgt
     1021 gttcattaat cgcaggcgat ggtgcttctt caattaattt ttggtgacgg cgttggatag
     1081 aacagtctct ttcgcccaag tgaacaacat ttccgaaact gtcgcctaaa atctgaacct
     1141 caatgtggcg cgggttttca aggtattttt ctatgtaaac gccggggttt ccgaaagaat
     1201 tttgagcttc gttttggcac atgttcatca ttatttcaag ctcgtcatct ttgcggacaa
     1261 ttctcattcc ctttccgccg ccgcctgccg ttgcttttat tataatcgga taaccgactt
     1321 tttttgcaaa gtctcttgcg tcatcaacgg ttttaaccaa atcggttccc ggggttatag
     1381 gaacattgtg ttccttcatg gtttttcttg cagttgcctt atcgcccatt tttctcatgg
     1441 catcagccga cggccctata aattttatcc cgtgctgatt acaaatatca acaaaatccg
     1501 cgcgctctgc cataaatccg taacccgggt gaatggcgtc tgccccgcac atcatcgccg
     1561 ctgaaattat cgcaggaata ctcaagtaac tgtctttgct ctgcgcagga cctatgcaat
     1621 aagcttctgt ggctaagctt acatgaagcg aatttgcatc cgcttcagag tgaatcgcaa
     1681 ctgtgggaat tcccaattca cgacacgctc ttattattct caccgctatt tcgccgcggt
     1741 ttgctattag tactttcttg aacatatgtt gtccttttat gattattcaa tttagttgaa
     1801 tagtttaaca gtcgaatagt cgaacagttt tgtttttatt aacttttcaa ctgttcaact
     1861 tttcgacttt tcaacttatt ctacgtacat caatacttgt ccgaattcga ctgcctgacc
     1921 gtcttcaacg caaatttcaa cgatttttcc tgcatgttct gattcgattt cattcataag
     1981 tttcattgct tcaataatac aaacaacctg cccctctgaa attgttgcac cgacttcaac
     2041 aaaaggttgt gcacccggag aaggtgcttt atagaaagtc cccaccatag gtgaagtgat
     2101 tgctttgccc ttaggcgcgg caacacaaac agaagattct gacaatcctg ctgtagaaga
     2161 tgtcattgta gctgttacaa cctgcggtgc tgctataact tctttttctt ttttaagcat
     2221 aatagctttt tcgccgtctt ctaaggcaat ttctgtcaaa gaactttctt ctaaaatttt
     2281 tataagcttt tttacataat ccatatcaaa attcataaat ttctcctttg ttattttcgt
     2341 tgcgaagcaa caaattgaac ttattcacct atttacctat tcacttattc acaaaatcta
     2401 gcatctgtcc aaatactcat tggttcttgt atcaactctt attctatcac cgttttgtac
     2461 aaaaaatgga acattcacaa tcgcacctgt ctctgttgtg gcgcgttttg tacctccttg
     2521 tgcggtgttt cctttttctg aaggcggagt gtccactact tccaattcaa cctgcattgg
     2581 catatcaaca cctattgttt tgccgtcgtg aagcaaaaca gatagcatca tgttttcttt
     2641 taagtatttt ataccctcgc cgatttgctc ttcagacagt gaaatctgtt cataattttc
     2701 catatccatc attacaaagc ttttgccttc tttgtataaa tactgcatat cgcgtctgtc
     2761 aagcattgct cttgcgactt tttcgcctgc tctgaaagtt ctttcaataa cctgccccgt
     2821 ttccacgttt ttaagttttg tccttacaaa agcagcacct ttgccgggtt ttacgtgcaa
     2881 aaactcaacc accgaccaaa gcccatggtc taagtctaat gttaatcctg tctttaaatc
     2941 gtttaccgat atcatgtctt ctcctgtaaa actcttgtta ccaacttata aatcccatgt
     3001 catgctgaat ttatttcagc atctttccaa agtcctaact gggatagatc ctgaaacaag
     3061 ttcaggatga caaagattct gatatttata ctatcataaa caaactaatt tgaaaatttg
     3121 ttaacatttg attgtcagca acaatttata gtattattgt tagattaatg aaaggtaaaa
     3181 tgtcaaaatt gaagggacag gtttttaaaa tacattctga tttctattat gtaaaattcg
     3241 cagataatgc gaagtttttt gaatgtaaaa tcagagaaat acttaaaaaa cagcaagaaa
     3301 aagttatggt aggcgatttt gtcgaatgtt ccgacattaa tcctcaaaaa cataccgcgg
     3361 ttattgaaaa tatttacccc agaaaaaatt ttcttacccg ccccagtgtt gcaaacgttg
     3421 aacaggtaat tattgtttct tcaataaaag aacccgacct taattttgag caattaaaca
     3481 gatatatctg tcttgccaaa caccataaaa ttaaaacaaa actttgcttc aacaaaaatg
     3541 acctgtctga agacgacagc gttattgaaa aaatatttga aatttacgaa cctttaggtt
     3601 ttgaaataat cttcacctct gccttggaaa aactcggcat agaagatttt gcccaaatgc
     3661 tcaaaggcaa aatgacggtt ttttgcggaa cgtcaggcgt gggcaaatcg agtttaataa
     3721 actcgattta ccccgatttg aaccttaaaa caaagcccgt cagcgaaaaa acagggcgag
     3781 gaattcacac gaccagacac tgcgaaataa ttgagattaa aagatgcgaa gatgcgaaga
     3841 tgcgtagata cggaaatatc cacgcatcca cgtatccacg catccacgcc cctattaaaa
     3901 tcattgacac ccccgggttc agcaatctta aatttgattt tcttttgccg acacaaatcg
     3961 atgaactttt tgatgaaata aagcagcaca aaagcgactg caaattcaaa gactgcctgc
     4021 atattcaaga agaaggctgc aatgttcttg cccacattga tgaaatcgac ctgtcaagat
     4081 accaaagcta ccttgctttt gttcaggaag caaaaggatt taaagaaaaa ataaaaaccg
     4141 aaggcacaaa gaaagaaagt tttcataaaa agaccttaaa caaaaaagtg gcaaagataa
     4201 gcaataaaaa aaggcaatcc gccagaaaca ctttaaaaca gcaattatat aaggatttag
     4261 aacatgcctg acaatatgcg cgaatacata gaactaataa ataaaaatct agataagttt
     4321 ttaaaaataa cctatccgca agaaatattt gagtcaatgc gctacagcgt tttagcagaa
     4381 ggtaaaaggc tcagacctgt tatgtgctta gaaagttgca gaattttcgg cgggaaaatt
     4441 gaagacgcga ttccgacggc gtgcgctatc gaaatgctcc atgcccaaag tttaatccac
     4501 gatgaccttc cttgtatgga caacgacgat ttcagacggg gaaagcccac aaatcacaaa
     4561 gcattttctg aggctaccgc tgttcttgca ggcgatgctc ttttgacttt agcacctcag
     4621 ataataattc agcaatccga aaaccttgat gctcaagtca aaattaaact tgttgaacaa
     4681 tattgcatag cagcaggtgc tttcggtctg attgcaggac aaattgtcga tattcaatcc
     4741 gaaggacaaa aaatttcgcc taaaaccctt gaatttatcc acactcacaa aaccgcggat
     4801 ttatttaaac ttgcgctcaa agcaggggca ataattgcaa aagcagatga aaaaataata
     4861 aaagcaatgg acgatttagg acagtgcttg ggctttgctt tccaaatctg tgacgatatt
     4921 ttggatgaaa cttcttcttt tgaaaaaatg ggcaaaacgt taggcaaaga taaagatgcc
     4981 caaaaatcaa cctacaccgc actttttgga gtaaaaaagg ccagaagtga agtgatttgc
     5041 cttttaaata aggctcatga tataatttca aaaaacaata tcaaatcggg gattttagaa
     5101 gagataataa aagggataca agaaaggtca aattcttaaa atgataaatg gaactcaaat
     5161 cgtaaataca ggttatgaag taatgcttac gggcattttt gcagctttaa ttgctcagat
     5221 aataaagttt ttactccatt tgattgtaaa aaagaaaatt gattttaaag tatttacgac
     5281 aacaggcgga atgcctagct ctcactctgc aggtgttgta ggactttcta ccgcagtggg
     5341 tctgatttcg ggatttgatt caataatatt tgccgtttcg gcaggatatg cgactgttgt
     5401 tatgtacgat gcggcaggac tcagaagggc ttcgggtaaa atggcaactt gcttaaacaa
     5461 aattatggac gatttttata aacacgatgt gcaatcggcg ggcggaaaat taaaagaact
     5521 tcttggacac actcctgttg aagtttttgc aggtgcactt ttaggctttg tttatgcctg
     5581 ttacatgcat ttttattttt taagctaaga gggaaaatga aagaagaaaa aatcataata
     5641 acaatttccg gtacagacaa aataggtata gtggcaacta ttacagctat tttggcaaaa
     5701 cattgtgtaa acatcgaaga tattaaacaa acacttatgc agggtcattt tgtgatgttt
     5761 atgcttggtg atataagcgc atcagaacat tcttttaaag aaataaaaga ggctcttatt
     5821 caagcgggaa aagaaatcca aatggaagtt tgggttcaaa gaaaagccat ttttgacaaa
     5881 atgcacacga tttaagaaat tctgttttat acaaatgcca cactatcctt ctcttaagtc
     5941 attgcgaggg ggtaaatgat ttgtgggggg tatgctaaag tcgcacgcgt cgttgcgagg
     6001 ggtaaatgat tttgggagta tgctaaagtc gcacgcgtag ttgcgaggag cgaggtgcta
     6061 accgagcgac gcggcaatct aactaattaa aatatatttt ttatttccta tttctgtggc
     6121 gtaaagattt ttggattgct tcggctgcgc ctcgcaacga caaaatgatt ttttattgtt
     6181 gggccggaaa gcccaaccta cttttctgga aagatcctga atcaagttca gaatgacgtt
     6241 ataaatttac cgtttactga caaaataaat tttattatta caatataatt ttttctacct
     6301 ttttaaatcc ggaagtgaat caacaaaatc aggaatcccg acttcttttg gattttcttt
     6361 ttgattttgc tcaacagtat ttttttgtac aaaattatct tctcttgcgg gagagttttc
     6421 aatatactgc aatgcttgag gcgcggctaa ccatttgaaa gacttaacag aactttggct
     6481 ttggactttc ccgtcaatta ttaccttaaa tatttttgta ggaagatttg tcttgggcgt
     6541 gagaggcgga attaagtcga ttttttcata atttgcattt gtggtaattt gtctttgagg
     6601 caaatcggtt tttgttattg aagaaattat tttgtcaaca gaaatatcgc ccaggggccc
     6661 tagaacttgg gctacatcat cagaaaccct tcctaaaatt gtcagatttg caacttcatc
     6721 tatcaaatga tatctccctg taatgtacat gctcattgaa ggacccgttg tggtaagaga
     6781 ggaaatattt gcccatgcct tggaaaaatc aacatcccct ctcatatact tataaactcc
     6841 tgtattttta gcacttacgg cttttgctat caaattaaga tttgttttaa acaaattatt
     6901 tgagacaata ttttgcgcat ataaaaggtg ttccagcttg cccagtgttc ccatttctcc
     6961 gtttgaaatt acaaattttg tattaccttt aagggttttt agaagctgat ttctgttttg
     7021 ccctgacatt gtgatgtttg aatcaaaatc aagctgtccg tgaatttctt ctttcatttt
     7081 actaagtgct ctaattgcca cagcagcact caaacccctt ccttgcaaat taagcccaag
     7141 cttttgccat ataaggtcgt aatttatact ccctgcaaat ttgcccaaat aagcatcgcc
     7201 tctgacatta tccatactga aaatattatt tcttaaggaa atatttgaag tgacatttgt
     7261 agttataata tctgccgcct taaatctgtc gagcctgctt gtgccgctta aaacagtaaa
     7321 agatgcatca gtttttttag gaagttttga tataagacca gtaaggtcgc tcaattcaag
     7381 gttttcggag gttatatcga cattgtttat aaccaagcct tgagaaaagt tgttcagcgc
     7441 aagggcattt atattaacga ttgaatttcc aaacaaacct tcaggacaag tcgcacttat
     7501 aaaattttgg ttaaaatgta tcattgaatt tttaaaaccg agttgaagct caggaattga
     7561 aagtgacgaa attgacaaat aaccttcgat atcaggattt ttgatatttc cgttcatggt
     7621 caaatcgcct tttaatcggg cagttgaatt aggataatca ggaagcgaaa ccgtaagctg
     7681 attaggaatt tttattccga tatctctgaa attcgcctca gaatttatat cagaaatttt
     7741 tccgcttaaa gttaagactt tgtagcctga tgaagtattg gttttaaaat tttccgaaag
     7801 agaatttatt gcttttgttg agtaaagggc aatttcttct attataatgt ctttatcaag
     7861 ggttaaatcg cagtttacaa gagaagtctg ccccagccct tttatatcaa cgaaagaaac
     7921 atggttgttt ttgtttgcaa gcatttgagc ctgaattttt tgtttttcac ctccgtaaag
     7981 ttttattata atcggaagtt tcccgattga ttcgggctta taattttttg tcattgaaga
     8041 aatgtctttt gccaaaatga agccttttgc tttaatgtca acaaagaagt ttggcgaatt
     8101 gtagttagaa atctttccgt agaaattcaa tttggaattg tccatgagga ttgaactttt
     8161 ttctatattt aaatcttttt tatcaaaact tacggacaat tcaggcaaca agattaattt
     8221 aaagaaattt tgggaagaaa tttttatgct gtttgcattg attttgccct taaattcttt
     8281 attttttatt gttgaatttg catttgcgct ttgtaccgat atttttgccc ggtgaggctt
     8341 attgtatatt ataaggttat taaaagaagt attaatttca ggcaaaagat tatccagcct
     8401 acctttaagc tttgtactta cacttacgga cccgcccaaa acgtcatttt gactcaaaaa
     8461 atcccttttg cccaatgtgg caagaagccc tttaagctgc aggtttttac catcaattaa
     8521 aatatcggcg ttagcatttt tatcaagact tccttttatt gcaataggct gtgaattaag
     8581 aatcgcgctt gcttttttaa tatttaccat gtcttgtgaa aaatcaatat ctgcaaatat
     8641 tgaacttaaa gacgcattgt ataatttata atccaatttt gcattttcga tttttaaata
     8701 accggcggaa tgaacagttt taaagtcgct ttttaatttg aaatccgcac tcaattttcc
     8761 gtcggcgtga attttatcaa acttttttat cccgaaaatt ctgcaaagat tgtttgctat
     8821 caaaaacaga tttgatattt ctgttttttt gcttttgaca tccaaattca gatatttttt
     8881 gtcaccattt tcaaaagtac cctcaacagt cgaattgtca ctttgagagg tgaaaatatt
     8941 tgaatcgatt ttaattttgt gcccgtcaaa ttttaaatta attttgctat ttggaagtgt
     9001 tactccgtct attgcagttg aaatattttc taaatcaagg ttcccgtcta tttttaaatc
     9061 ttgtttttca ccgcttattt ttaaatcgga ttttatctgc gcaaggaatt tatacctgta
     9121 caagtcttca aaaagtttta ctatattaaa atccgatacg gttttagatt tttgaaaatt
     9181 atattctgga agaacttttg taaaaacaca aatgtcataa gtgatttgtt ttcgattatc
     9241 caaaaatagt gcgccctgcg cttttagctt aactttctta tccaaaacaa aatcgcttaa
     9301 ttgaaagttt tttcccttga aaaaataccc ctttgacgta tcggtattga ttaaagaaac
     9361 cctgtatttt tcgattttta tatccggaat agaaggataa aatgaaaatc gtaaaggcag
     9421 tttttgccca taggcttctt caaattgaat gggttttaag tatttttcaa gcaaaaattg
     9481 ccctttactg ttaactttta gattaaacaa tgctttttga gaatcaagcg tagtgagttt
     9541 tatttttccg aaaataagat acggaacaga tactgctatt ttaaaatcgt ttatctgccc
     9601 gaacttttca ccgtttggat aagacaaatc aaccctttgt gccgttgcgc cggcagaaag
     9661 cttccagtcg gaaaatgttt ttgcgtcttt aaattttatg actataccgg ttttttcctg
     9721 aattgtttgg gttatttgag gcaagtagtc attaatgtca aaaaaggtcg gcagcgcata
     9781 tatgaatgcc aaatataaca atatgcaaaa agaaaaaacg gttattaaaa ttatttttga
     9841 gggtttcatt attccagcct tt
//
LOCUS       DAOQAU010000059         9547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_8422_length_9547_cov_13.237147, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000059 DAOQAU010000000
VERSION     DAOQAU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8422_length_9547_cov_13.237147"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1079
                     /locus_tag="PKI94_07635"
     CDS             438..1079
                     /locus_tag="PKI94_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNW26642.1"
                     /translation="MKLPKVICIACGKGGVGKSTTSINLAVGLSARHAVELLDLDYQK
                     TATIFNRLRTDEGHEPLAVKTADSETQVKNVIKKCKGILIIDTGAFDTPTNRRAMLMA
                     DMVITPMSDSIVDLYGLMMYEKVIRELKEADKNFKVYILPNKIEPRNNDMTEITAVVK
                     KHKANFTLLKSIIFNRKDYRNAFENGLGITELKKATAGEEFTKLIKEIEKCLK"
     gene            1067..1276
                     /locus_tag="PKI94_07640"
     CDS             1067..1276
                     /locus_tag="PKI94_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26643.1"
                     /translation="MSKIKEMDIFDATHKAKLTEAKHESDMLKAINIYNVPKEWIELI
                     KAEGQTFSSFAKLAIKKELKAMGLI"
     gene            complement(1297..1602)
                     /locus_tag="PKI94_07645"
     CDS             complement(1297..1602)
                     /locus_tag="PKI94_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26644.1"
                     /translation="MKCGCGGSFKEKCKEIQGIKCDVMVCDSCDDIVFTIDQSKKYHK
                     MRLIQDELSKEFKKISKVGNSMGITLPAKLKELGFDIGKILDIRLIDANKLMIELHI"
     gene            complement(1599..1895)
                     /locus_tag="PKI94_07650"
     CDS             complement(1599..1895)
                     /locus_tag="PKI94_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26645.1"
                     /translation="MIKMSIFEKLYMQRLSVIDNVVLSVHCVIDKMPALNLDETDIMK
                     AYKHGKLYLEKCERPEKIALSWYDGKQRRTIVLIVNIFENYAKVVTVWEQKGKL"
     gene            complement(1971..3260)
                     /locus_tag="PKI94_07655"
     CDS             complement(1971..3260)
                     /locus_tag="PKI94_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HNW26646.1"
                     /translation="MNSEKAMMIKKNSALIQVHAKTLSAIQRKIINSLIYTMQRTTGQ
                     QGSYNITISALKKFCNISMIGNNELKDSFKGLADIKLEFNYLNKDKNEVWEYMSLLSR
                     VKIVPNEGNIIFELPEFLRDKLINPRLYAPIDIVLIAGFKSSYSIVLYELMRDYLKAP
                     SVPMMTIEDFRNLLGIKSNEYKCFNDLKKRVLDVAVKEVNEKTDICCNYDLIKEVGIR
                     NKYSHIHFFVSKKPVKPAYEENETIKIKDDADYSMDLFEESVSLVNKTVVQIPEGIMS
                     VVPEDHRTAALKTLLSRFLEKGNEYVISNIKYSLEHFQENFIGYLKVALENDYACHVR
                     EAQANIREAAVKKSRQIEQKEKAKQAVHNDHAEILKKIENLVPDKLKPIHNKFDEIVT
                     KSGIKPEFLTDTVKESLMIEAYKALHVVNAYSAPDQD"
     assembly_gap    3328..3427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4098..6665
                     /locus_tag="PKI94_07660"
     CDS             4098..6665
                     /locus_tag="PKI94_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26647.1"
                     /translation="MTDKFKLFSLIIALFLVLSVLDHSKAGISEVKQSLKKDSISSKS
                     TNEAKLKNKSPLPKSKSKTNIKLKAELPEIKKDYPNSFLKPSEVPYKIVKKTINGKIQ
                     VSRFPFIPDKDTNKVKQPQRISDSKTAKRIGEKNKNLDELTGVEGELLPSNWEFKEPP
                     ITNTALPSIPDNNDSRKKEALALLKYISQNLPDYSDPTQKNPNPQMPGISKYCYSLCE
                     NYSDYPEICAYARLYQAEAWMQGVQLTLTKYQIESCRIALNRLNESIDIYSNFAKNNP
                     DCAAAVQIMKGRAKLLQDCIDKHVQYLGYENKYYYLPRANRHLGNLKRIKSDVLKPND
                     DLYSSDDSSISGIHDQTTSYISTILMNIYLMNDTSQFKAVEAQLDSFYKSIPREITIK
                     FREDFDENSAVKERTLTVTKTGDNYVNAKRYNAYMIVENCEKDEKLNIESYHSQFEEM
                     NQYASSSHDGEAYYTNTREILKEYLSILNIPLETTAVYFCHAEQQIEDGKFYEQEDLL
                     MLELDINANSLQPLFPIREKIVNATTHKRKTLVIKRSSLYANSYCSGLFRTVDIIDND
                     DEDTPIPPPSNDQPTLALKKSDVLHVDERSIGVFQGEPEYFKDYLSSALIHQLDVPPD
                     LYDKTKCSYFNKRLVRTYIENKKYNSIDNSDKNNKNYLLCGGSEYVYYPKGNAYVPTK
                     KTAMFFIIQGHSANRNGAEVGEKSNDGIYVTPEELSKSKRYQDNMKVLILDACFCLKF
                     YKTWNEVLPNGVILGYNDKVGFRSMNNVLGLMANELDKASTGITPNQIAGKWLKMNEK
                     SFNTYLADKTRTEDKRPKCTACVVDDVDEPGKRAYVEFELYLDEHNNKHFLYKATTF"
     gene            6677..7552
                     /locus_tag="PKI94_07665"
     CDS             6677..7552
                     /locus_tag="PKI94_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26648.1"
                     /translation="MKRFFLRCIILFMILLFLHTIVYAKSPKNKDEFFEIFSIANKKA
                     YEKTDVYINKVINLFRDYPNSLDAYFISCSLDNIIDANDNYKQVFPKFEELYKKYYDT
                     INNLDTDTSEKVLLSYMLTRGFSFVEGNEEEMEKNSALGYEILNNIKNNCINDNYKAL
                     AILLIANVNPDLTMLTEFKDKYPNNSALPGVEFSIVDKLYAGTNETQFINELLNLKKI
                     HYNVESPFGHKFYIHLYSSIVFAYMKLNDINNAKIYFELIKSEAPNYWNIQTLEKFLG
                     ESTKNKSLFNSMFGH"
     gene            7589..7939
                     /locus_tag="PKI94_07670"
     CDS             7589..7939
                     /locus_tag="PKI94_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW26649.1"
                     /translation="MSAEIMTIKELSAYLDITEATIYKKVSNNEIPYTKLGTLLRFPK
                     WAIDSWISQNTVEPPDALFNEFAKLQSRYHFKKWLESKGVDTSAVTDQQLLELVKKAI
                     ADLSSDNPPKKKSR"
     gene            7936..8979
                     /locus_tag="PKI94_07675"
     CDS             7936..8979
                     /locus_tag="PKI94_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26650.1"
                     /translation="MTDVRLLTYARKLARLGQDEVLKELGIKHFKKLILKMPNEELLK
                     EFGKSAKNINKSLFIKNVIWQTYEQIKAGQKPFDIGNIRSFWYYIKDTMDRVGANKKG
                     DAYTIVSNMFAVMVKAGLFKYRDFGFDDDDKGNRWLGGKYARVILFAEKTSYTDLLQE
                     VNRDFDITTIAVGGQSSYLSIEYFVQELADQGVDLTKEFIIFSIVDFDPAGDIIATNF
                     TDNLEDNGIKTIKVFAGQFYKNFKRNDIMIPANMTPDQRKRVYRLPLSVRKSGQAAKW
                     AARTGGIDGKKNINFGIETIVMPKAQLLELFERELAKVMTIDLEAIAKFREMKNLKVQ
                     MQAWAVKKLQLEG"
     gene            8991..9206
                     /locus_tag="PKI94_07680"
     CDS             8991..9206
                     /locus_tag="PKI94_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26651.1"
                     /translation="MFDKLVMRDICGHQVPAVEIDTPHGQIYAYKIKDEFVYEFPSGR
                     ARRGAQKFFNGSAEDIKTLLKILQDQL"
     gene            9217..9390
                     /locus_tag="PKI94_07685"
     CDS             9217..9390
                     /locus_tag="PKI94_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26652.1"
                     /translation="MEEKEISVSGKQQPQEDFWNKISGYVVAFLFGAFTTFVILYWMF
                     SPMFMEIGNHGAK"
BASE COUNT         3267 a         1694 c         1688 g         2798 t
ORIGIN      
        1 tccggcgcgg gcctacacac ggccttcggc tactttgtat tttaatagag aaaaagaacg
       61 ttcaagccaa gctctgaagc acggagaaga tcgagcgggc cgacgacgaa aaacggaatg
      121 cattagagcg cgttaaaagc catccggaag aaaaagacga cacttgacca agccggccag
      181 acaaacgcgc cttaaaatca aattgtgcaa cctcaaaaat aagataacac agctatattt
      241 tataaaattc atttcaatat caaaatgaag ctgttacaac tttaacacgt cagcttcaaa
      301 acaacttcac ttcaatatta taacaataca cggtaaatga agttaaaaca atatcatttt
      361 agcttcgaaa caacactgtt aaaataatgt ttgacaactt cattatgaag tcgatataat
      421 acctgggtga agttaatatg aagttaccga aagttatatg tatagcctgc ggcaaaggtg
      481 gcgtaggaaa atcgactacg tctattaatt tggctgttgg attatcggcc agacacgctg
      541 ttgaattgct cgacttggac tatcaaaaaa cagcaacaat cttcaaccgg cttcgaaccg
      601 acgaaggcca cgaaccgtta gcagtcaaaa ccgcagatag cgaaacacag gttaaaaacg
      661 ttatcaaaaa atgtaaagga atactgataa ttgacaccgg cgcatttgac acgccgacca
      721 accgacgcgc catgctcatg gccgacatgg taataacgcc tatgagcgat agcatcgttg
      781 atctttacgg tctgatgatg tatgaaaaag tgatcagaga attgaaagaa gcagacaaaa
      841 attttaaggt ttacatttta cctaacaaaa ttgaaccgcg caataacgat atgacagaga
      901 tcaccgcggt cgtaaaaaaa cataaggcta attttacttt gttaaaatca ataatattca
      961 acagaaaaga ttatcgaaat gcctttgaaa atgggctagg aataactgaa ttgaaaaagg
     1021 caaccgccgg agaagaattt acaaaactta taaaggagat tgagaaatgt ctaaaataaa
     1081 agaaatggat atattcgacg ctacacataa agccaagctg acagaagcca agcacgaaag
     1141 cgacatgtta aaggccatca acatatacaa cgttccaaaa gaatggatcg agctgattaa
     1201 ggcggaaggc caaacttttt caagctttgc aaagcttgcc atcaaaaaag aattgaaagc
     1261 catgggtttg atataaccct ggccaaaaga tggtatctat atatgaagct cgatcatcag
     1321 tttgttcgcg tctatcagtc taatatccag aatctttcct atatcaaaac cgagttcttt
     1381 cagtttggca ggcagtgtta ttcccattga attaccaact ttgcttattt ttttaaattc
     1441 cttagatagt tcatcctgaa taagcctcat tttatgatac ttttttgatt gatctatcgt
     1501 aaaaactata tcatcacaag aatcgcaaac cataacatca cattttatgc cctgtatttc
     1561 tttgcatttt tctttgaaag atccgccgca accacatttc ataattttcc tttttgctcc
     1621 catactgtca ccacctttgc ataattttca aatatattca ctattaaaac tattgtcctt
     1681 ctttgctttc catcatacca acttagtgca attttttctg gacgttcgca cttttcaaga
     1741 tacaatttcc cgtgcttata tgctttcatt atatcggttt cgtccagatt aagtgccggc
     1801 attttatcga ttacgcaatg aacgcttaaa acaacgttat cgataaccga aagtcgctgc
     1861 atataaagtt tttcaaaaat tgacatcttt atcacacttc ccgattatgt cattacctat
     1921 attattacac atgttattac aattgtcaat acttatcaat ttgttgtcca ctaatcttga
     1981 tccggcgcgc tatatgcatt aaccacatgt agagctttat atgcctcgat catcagcgat
     2041 tcttttaccg tatccgttaa aaactctggt ttgataccac tttttgtaac tatttcatca
     2101 aatttattat gaattggctt aagtttgtct ggtacaaggt tctcaatctt ctttaatatt
     2161 tctgcatgat cgttatgcac ggcctgcttt gctttttctt tttgctcgat ttggcggctt
     2221 tttttgacgg cggcttctct aatgtttgct tgggcttcac gaacatggca agcataatca
     2281 ttttcaagag caacttttaa atagcctata aaattttctt ggaagtgttc caatgaatac
     2341 ttaatatttg aaataacgta ttcgttccct ttttccaaaa atcgacttaa aagagttttt
     2401 aaagctgcgg tcctatggtc ctctggaacc acggacatta tgccttccgg aatttgaacc
     2461 acagttttat taaccaggct aaccgattct tcaaaaaggt ccatgctata atcagcatca
     2521 tcttttattt ttatagtttc gttttcttca taagctggct taactggttt cttgcttaca
     2581 aaaaaatgta tatgcgagta tttatttctg attccaactt ctttgatcaa atcataattg
     2641 caacaaatat cagtcttttc atttacttct tttacggcaa catcaagcac ccgcttcttt
     2701 aaatcattaa aacacttata ttcattgctt tttattccca ataaatttct aaaatcttca
     2761 atagtcatca ttggcacgga aggagctttt aaataatctc tcatcaattc atacaaaact
     2821 attgagtaag aacttttaaa gccggctatt aaaactatat ctatcggtgc atataaccgc
     2881 gggtttataa gtttatccct taaaaattcc ggcagctcaa aaataatatt cccttcattt
     2941 ggaacaattt taactcgaga taataaagac atgtattccc aaacttcgtt tttgtcttta
     3001 tttagataat taaattcaag ctttatatcg gcaagccctt taaagctatc tttcagctcg
     3061 ttattgccaa tcatgcttat attacaaaac tttttaagag cagaaatagt tatattgtag
     3121 ctaccttgct gacctgttgt tctttgcatc gtgtaaataa gagaattaat gatcttgcgc
     3181 tgtattgctg atagcgtttt tgcatgcacc tgtattaatg cgctgttttt ttttatcatc
     3241 attgcttttt cgctattcat ggttagctcc ttatcttttt aagatcacag ttgaattata
     3301 ccaaacacgg acaagattgt caatacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnntgt cggggttagc tactgaaaat gtcggggtta gctactgaaa atgtcggggt
     3481 tagctactga aaatgtcggg gttacttcga ctatttcctt actctcccaa cgtttttatt
     3541 ccggtttaaa cggttctaaa cggtattaaa cgtacttaaa gaaacaacaa caggtaactt
     3601 cgcgcgcgcg tacgcgtata taataatata tataccgccc caaaagttgt tgttgttatt
     3661 tttttcaaaa ataggagacc gggcatcaaa aaatgaaaag tggcataaac aaaggatagt
     3721 tttaaaaaca ctatatttca ttggtgtttt agacgctagt tgaggatcac cgacgataga
     3781 gtgaaaagcc ttataaaatg aagtacatta aaaaagccta atattaggca gcctagaata
     3841 gaagttgaat ataacgagaa gccgcggggc gctaataatc cgataaatcc tgtccagtgt
     3901 gataatatac aatatgttaa ccggcaataa gcttttgaag ctctgataac cgccagattt
     3961 gctataaccc gcaaaaaaaa tattatcgca aagaaaggcc actggacgga aatcacgaaa
     4021 taagataaaa attatcgcag ttgacgaaca aattttttta aagtataata tcagcatctt
     4081 cgtttgagag gtattaaatg acggataaat ttaaattatt ttcgttaata atagctttgt
     4141 tccttgtttt atctgttttg gatcattcca aagctggaat aagtgaagtt aagcaatctt
     4201 taaaaaagga ttcaattagc tctaaatcaa ccaatgaagc aaagttaaaa aataagtccc
     4261 ctctaccaaa atctaagtcc aaaacaaaca taaaacttaa agcagaatta ccggaaataa
     4321 aaaaagatta tccgaactct tttttaaagc cttctgaggt tccttataaa atcgttaaaa
     4381 aaacgattaa cggtaagata caagtttcac gatttccttt tataccagac aaagatacaa
     4441 ataaagtgaa acaacctcaa agaattagcg actcgaaaac tgcaaaaaga attggcgaaa
     4501 aaaacaagaa tttagatgaa ttaacgggtg ttgaaggaga gttacttcct tcaaactggg
     4561 aatttaaaga accgccaatt acaaatacag ctttgccaag tattcctgat aataacgata
     4621 gtagaaagaa agaagccttg gctcttttaa aatacatatc ccaaaatttg ccggattatt
     4681 ctgatcctac acaaaaaaat cctaaccccc aaatgccagg catctcaaaa tactgttatt
     4741 ctttgtgtga aaattactct gattatccag aaatatgtgc atacgcacga ttatatcaag
     4801 ccgaagcctg gatgcaggga gtacaattaa cacttactaa atatcaaatc gaatcatgta
     4861 gaatcgcgtt aaatcgatta aatgaatcta ttgatattta ttcgaatttt gcaaaaaaca
     4921 atcctgattg tgcagccgca gttcaaataa tgaaaggacg tgctaaatta ttacaggatt
     4981 gtatagataa acacgttcaa tatcttggtt atgaaaacaa gtattattat ttacctcgtg
     5041 ctaacaggca tttggggaat ttaaaacgca tcaaatcaga tgttttaaaa ccaaacgatg
     5101 atctatattc ttctgacgat agttcgattt ctggtatcca cgaccaaaca acaagttata
     5161 tttcaactat attgatgaat atttatttga tgaatgatac aagccaattc aaagccgtag
     5221 aagcacagtt agattctttt tataaatcta ttcctcggga aataactata aaatttagag
     5281 aagattttga tgaaaattcc gcagtaaaag aaagaacgct tacggttaca aagaccggtg
     5341 acaattatgt aaacgctaaa cgttacaatg cttatatgat tgtcgaaaat tgcgaaaaag
     5401 acgaaaagtt aaacatagaa tcttatcaca gccaatttga agaaatgaat caatacgctt
     5461 cttcatcaca tgatggcgaa gcatattata caaataccag agaaatttta aaggaatatt
     5521 tatcaatctt aaacatacca cttgaaacga ctgctgttta tttttgtcac gctgaacaac
     5581 aaatagaaga tggtaaattt tatgaacaag aagacttgtt aatgttggaa ttagatatta
     5641 atgccaattc attacaacca ctatttccta taagagaaaa aattgttaat gcgacaaccc
     5701 ataaaagaaa aacattagta attaagcgtt caagtttata tgcaaacagt tattgtagtg
     5761 gtctttttag gacggtggat attatagata acgatgatga agacactcca ataccacccc
     5821 cgtcaaatga ccaaccaact ttggcattaa aaaaatcgga tgttttacac gttgatgaga
     5881 gaagtatagg tgttttccaa ggcgaacctg aatatttcaa ggattatcta agttcagcat
     5941 tgatacacca acttgatgtg ccaccggatt tatatgataa aactaaatgt tcatatttta
     6001 ataaaaggct ggtaagaact tatattgaaa ataaaaaata caattctatt gataactcgg
     6061 ataaaaacaa caaaaactat ttattgtgcg gcggttctga atatgtatat tatcccaagg
     6121 gaaatgcgta tgttccgaca aaaaaaacag ctatgttttt tattattcaa ggtcatagtg
     6181 ctaatagaaa tggtgcggaa gttggtgaaa aatcaaatga tggaatttat gtgacaccag
     6241 aagaactgag taaatctaaa cgatatcaag ataatatgaa agtattaata ttagatgctt
     6301 gtttttgctt gaaattctat aagacgtgga atgaagtttt accaaacgga gtaatactgg
     6361 gctataatga taaagttggt ttcagatcaa tgaacaacgt gctgggtcta atggccaatg
     6421 aactcgataa agcgtcgacc ggcataactc ccaaccaaat agctggaaaa tggttaaaaa
     6481 tgaatgaaaa aagcttcaat acttaccttg cagacaaaac tcgtacagag gataagcgac
     6541 caaaatgcac cgcgtgcgtt gttgatgatg ttgatgagcc gggcaaaaga gcttatgtag
     6601 agtttgagct gtatttggat gaacacaata acaaacattt tttgtacaaa gcaactacat
     6661 tttaaaggag gatatcgtga aacgtttttt tttaagatgc atcatattat ttatgatatt
     6721 gctattttta cacactattg tatatgcaaa atctccaaag aacaaggatg aattttttga
     6781 gatattcagt atcgccaata aaaaagcata tgaaaaaaca gatgtgtata taaataaagt
     6841 aatcaacctt tttagagatt atcctaattc attggatgct tattttattt catgttcact
     6901 ggataacatt atcgatgcca atgacaatta taaacaagtt ttcccaaaat tcgaagaatt
     6961 gtataaaaaa tattacgaca cgataaataa tttggatacg gatacttctg aaaaagtact
     7021 gctatcctat atgcttacta gaggattttc ttttgtcgaa ggcaatgaag aagaaatgga
     7081 aaaaaattct gcacttggtt atgagatact taacaatata aaaaataatt gtattaatga
     7141 taactataag gcgttagcca tattattgat agcaaatgta aatccagact taacaatgct
     7201 tacggaattc aaagataagt atccaaacaa ttctgcgtta ccaggtgtag agttttcaat
     7261 tgttgacaaa ttatatgctg gtaccaatga aactcaattt ataaatgaac tacttaattt
     7321 gaaaaaaatc cattacaatg ttgaatcacc atttggacat aaattttata ttcatttata
     7381 tagttcaata gtatttgcat atatgaaatt aaatgatatt aataatgcaa aaatttattt
     7441 tgaattaatt aagagtgaag ctccaaacta ttggaatatt caaactctgg aaaagttttt
     7501 gggagaatct actaaaaata aatcactttt taattcgatg tttgggcatt aaatttttaa
     7561 tggtcattta acaaaatatg gagtaacgat gtccgcagag attatgacaa tcaaagagct
     7621 ttcggcctac ctggacataa cggaagccac gatctataaa aaagtttcta acaacgagat
     7681 accatatacc aagctgggaa cgcttttaag attcccaaaa tgggctatag acagctggat
     7741 atctcaaaac accgttgaac ctccggacgc gcttttcaat gaatttgcaa agcttcaatc
     7801 acgctaccat tttaaaaaat ggcttgaatc taaaggcgtg gacacttccg cggtcacgga
     7861 tcagcaatta ttagagcttg tgaaaaaggc gatcgccgat ctatcgagcg ataacccgcc
     7921 gaagaaaaaa tcaagatgac agacgtgcgc ttattgactt atgcccgcaa gttagccagg
     7981 ctcggtcagg acgaagtctt aaaagagctt ggcattaagc atttcaagaa acttatcttg
     8041 aaaatgccga atgaagaact tttaaaagag tttggaaaaa gcgcgaaaaa tatcaacaaa
     8101 tcacttttta taaaaaacgt tatatggcag acctacgaac aaataaaggc cggccagaag
     8161 ccttttgata ttggcaatat ccgctcattc tggtattaca taaaagatac gatggaccgc
     8221 gtcggggcca acaagaaggg cgacgcttac accatcgttt caaatatgtt tgccgtgatg
     8281 gtaaaagcgg gccttttcaa gtatcgtgac ttcggtttcg acgatgacga caaaggcaac
     8341 cgctggcttg gtggcaagta tgccagggta atcttattcg ctgaaaagac ttcatataca
     8401 gacttgttgc aagaagtgaa ccgagacttt gatataacga caatagcggt tggcgggcag
     8461 tcttcatatt tgagcattga atatttcgtt caagagctcg cggatcaggg cgtggattta
     8521 acgaaagagt tcataatttt ttcgatagtg gactttgatc ctgccggtga tataatcgcg
     8581 acgaatttta cagacaatct tgaagacaac ggcataaaga ccataaaagt ttttgccggc
     8641 caattctata aaaatttcaa gcgtaacgat attatgatcc cggcgaacat gacaccggat
     8701 cagcgcaaac gtgtttatcg gctgccgttg tctgtccgca aatccggtca ggctgcaaag
     8761 tgggccgcgc gcaccggcgg tatagatggc aagaaaaata ttaatttcgg gatagaaact
     8821 atagttatgc ccaaagcgca gctgctcgag ctatttgaaa gagagttggc aaaggtcatg
     8881 acgatagacc ttgaagcgat agcgaagttc cgcgagatga aaaaccttaa agtccagatg
     8941 caagcctggg cggttaaaaa acttcagttg gaagggtaaa aagcgtaacg atgtttgata
     9001 agttggttat gcgcgatata tgcggccacc aagtgccggc ggtagaaatc gatacgccac
     9061 atggccagat atacgcatac aaaattaagg atgaatttgt ttatgagttt ccgtccggac
     9121 gagccaggcg cggtgctcaa aagtttttta acggaagtgc cgaggatatt aagacgctat
     9181 taaagatttt gcaagatcag ttgtgagagg tgagccatgg aagaaaaaga aatatccgtc
     9241 agtggtaaac aacagccgca agaggatttt tggaataaaa tttcaggtta tgtggttgcg
     9301 tttcttttcg gtgcttttac tacctttgtt atattatatt ggatgttttc tccaatgttc
     9361 atggagatag gcaaccacgg cgccaagtag aatctgcccg aaccagcgcg ccgcgttcct
     9421 ggctcaatct taacgcacat ataccagata actaaagaag cgcaaaccct tattaaatca
     9481 agaaagtaac ggattgcatt tacctaagtg ccgattaaat cgacacttac gctacaagac
     9541 acatctt
//
LOCUS       DAOQAU010000060         9358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_8667_length_9358_cov_8.184349, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000060 DAOQAU010000000
VERSION     DAOQAU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8667_length_9358_cov_8.184349"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PKI94_07690"
     CDS             complement(<1..971)
                     /locus_tag="PKI94_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26653.1"
                     /translation="MNFEKTDEDLTIQLPTEDEKTLVQKIYQKYDELEDDRRIQLDDI
                     KLVRNAIYTNQVPQINGWDSNIELPDIYELAQTLKSHISDNLYSHPDAMFDVSGVTPQ
                     SQAFANRQKALLVNTFEQMKLEDELEKVIDGIVETGESTLFVGWETKIKSIRRAQSIE
                     EQLIAPTQDGFVIENQLIYDNPKVKYIKPEDFVFDKNRRDSWDSCTKIYRTYLDVEEI
                     SANKANNLLDEVKIEILKGVVADKTRKETDKSVCDNRLEILEYWGDIELTTGEILKNW
                     LIVTAGRREVIRFEANPFIINPFIHANIIENPSTGRGLSPLRVALIL"
     gene            complement(1153..1704)
                     /locus_tag="PKI94_07695"
     CDS             complement(1153..1704)
                     /locus_tag="PKI94_07695"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNW26654.1"
                     /translation="MSKFIKIIINKTRKEFKNIEYIPEVLNLYKRFANYLHDDYFIQK
                     YEDSIDLILNLIEKTSPYFWVVIHKKSNKVAGFAFLDNFIGAENNFHSAEITTCFNPS
                     FWGNYTKFCAKKFIKYCFKKYKLKKLKATIFPQNFKVKALLKRSGFKKEALLKAETLR
                     NGVLQDIEVYSIVNGLTGEQVKS"
     gene            1766..2224
                     /locus_tag="PKI94_07700"
     CDS             1766..2224
                     /locus_tag="PKI94_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26655.1"
                     /translation="MNSEEIMFQQYRLYTEQKEKFVDRSFHTNKFYLLVVLGLFLIMF
                     LLKGFAFAFDISAILVFSAAGMAICVLWWINMDAYNFLIKVKLGKVIEEIEKQLPSQP
                     YTQEFLAIKDLRKNKRMFLFADMQKILAIFAFLTFLILFVNEVIPLFIIK"
     gene            complement(2428..2784)
                     /locus_tag="PKI94_07705"
     CDS             complement(2428..2784)
                     /locus_tag="PKI94_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26656.1"
                     /translation="MKKEEKEIEQKLLSEVSLDELIKKKMEEEIKHEFEISKQKPQKN
                     LITDITKVPPHLIFSKAAIYKIFNRLNKTQTFINGIQAEAMLGLQNSLRDKIRLGQID
                     AFSTQDAYVKFEGVEM"
     gene            complement(2795..3217)
                     /locus_tag="PKI94_07710"
     CDS             complement(2795..3217)
                     /locus_tag="PKI94_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HNW26657.1"
                     /translation="MTQQQKKFVAEYIKTLDLELSAKNAGYKTKDYKNVAQKLLSDKL
                     IINEIKNQIKRQISSLVIQKGYIVKKLLDIVEFSLEEEDILDKDAQPSGKKKLRDTTA
                     GLRALESLCKHLGFANKSDEEPSETKIITISNLDEDKI"
     gene            complement(3440..4078)
                     /locus_tag="PKI94_07715"
     CDS             complement(3440..4078)
                     /locus_tag="PKI94_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26658.1"
                     /translation="MNYFELINKCLVELNYRKVTSFSELSKNDHEKIKNILNIINSEV
                     CGFDNWSFLLRKTNLTLPANCPEIENTINGRINSVFINNIKYEYCGDFEKFILNTQPS
                     KTFSAFNDKLLFPLFNQEKTVEIIYYTNFHAQDSQGSSIPKMSLEQDSSIIPDAYAEP
                     LLVYGTCMRVKANPEHKKFNYWYGMYKDALANMRSKIGTNALETPEIKLSRN"
     gene            complement(4160..4972)
                     /locus_tag="PKI94_07720"
     CDS             complement(4160..4972)
                     /locus_tag="PKI94_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26659.1"
                     /translation="MSQTENEKESSCAESQNLQAKPAEKEIKSSQAEGKEQKDSDTYN
                     QVLEKLEIKPENKDIQEKSAPVETRREDTDFYKNQENLHIQKEVENVIAQDFQKIESL
                     MKFGLINSWQGQNLKNQIIKKAFDLVLQNEKIKQNPSPAFKKDEVFSDFETNNPNFFQ
                     PEGRKEVLDYLKSDAVIIGKDDLKKISDMIENIEKTAIERYLQKDSYEKNLQNSNELA
                     KQKLTANAQNSNFQDKNLSRTFTREQIGKMSSAEFTKYEPLIMEQLKKGLIK"
     gene            complement(4973..5227)
                     /locus_tag="PKI94_07725"
                     /pseudo
     CDS             complement(4973..5227)
                     /locus_tag="PKI94_07725"
                     /inference="COORDINATES: protein motif:HMM:NF021218.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, LLH family"
     gene            complement(5322..6179)
                     /locus_tag="PKI94_07730"
     CDS             complement(5322..6179)
                     /locus_tag="PKI94_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26660.1"
                     /translation="MSDQTVNAYSAFIPEIWSQKLNTMLSKNCVMLQCVNRNYEGEIK
                     NQGDCVKIITPAEVTVSTVSSSAITYNELSPSTTDLIIDQKKFFAFKINDVAQAQANQ
                     SIMEAHLQNAKKAIEQVQDTYLLSQHAYADSSNVVGTEQSAVTLSKTTIYENFVNLAL
                     KLKNADALSNNQKPWVVINPSIESYLLQSPEFISAHNVADETLREGAIGRIAGMDVLV
                     STNLSAVGGKFYVLAGTNEAITFASQLAKIESLRDKDSFSDLVRGLYLYGAKTVVPKS
                     LAKMIVTAV"
     gene            complement(6236..7756)
                     /locus_tag="PKI94_07735"
     CDS             complement(6236..7756)
                     /locus_tag="PKI94_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26661.1"
                     /translation="MTDTFYNLGGGIKQSSTKTELGLDTNKLYWSDSKNIEILQNKGL
                     VRQKGNILIAKDKNSEKITGLHQIKMNKKNFILCSSEKGTFCIFDFESEEPAKIPIEF
                     QDSKTVNFTDYLNGVIISSKSDTLYYMKKNETFDVYDCQLTDEEGIPVFTNIVAVYKG
                     RVWAAKDSVIYFSALGKYDDFTTEGDAGFIKDFHTDTDEIIALKPYKDYLAIYKKNMV
                     YLLTGSNPDEFTIIPFANKGAAAVNGVINVNNKQYFINQGIFALEQVGELNQIQLGNE
                     ITLNIKSEFEKFDTERFDEIIALNYEKKNQVWYFIPYKNDDYFHTIWINDYVNKAWLK
                     RILPQDITTACIFKDDILTADKEGNIYKEDFGNTFDGHAIEFMWKSPFLCAGPTNSRK
                     TIDEFYFVVDEGYDNYFKFATYKNYDSETKDDADIIFSEVITTLVWDSETNTEKNSYW
                     DDDSEHISFWSANIDSTYKAEISESNYSVQLCVEGDSIEHDMAIIGLEFKEVYLDE"
     gene            complement(7772..8260)
                     /locus_tag="PKI94_07740"
     CDS             complement(7772..8260)
                     /locus_tag="PKI94_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNW26662.1"
                     /translation="MKRIILHWTAGGPVPTSYEKECYHFLVDTLGKIHLGKFKPEANL
                     ECKTGMYAPHTGGGNTGSIGVAMCAMANFKDTKNFGQFPITRIQFEATMKLCAQLAKK
                     YDIDICPQNVMTHYEFGQAHPNTTSKGKIDIIFLPPFCWIKKDDIGSFIRSKIRWYVE
                     NE"
     gene            complement(8270..8479)
                     /locus_tag="PKI94_07745"
     CDS             complement(8270..8479)
                     /locus_tag="PKI94_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNW26663.1"
                     /translation="MMKLSNRELEILELLSFGYSDKEIAYILNISARTVQTHVMRSVI
                     KLNARNRTNAVANYIRHYSLNFSTV"
     gene            complement(8674..9054)
                     /locus_tag="PKI94_07750"
     CDS             complement(8674..9054)
                     /locus_tag="PKI94_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26664.1"
                     /translation="MGYIHCCGAKRKSKSYTLVPEKNYLLVELDYLIKCPACGHTVVQ
                     ITRIDFNHDISFVRKVNEKAKKLFENLQNSILCKKEPEYKSIKVFSKFYLNYNEFGVK
                     KKCYSNLSSLKIGLFENNPYINQS"
BASE COUNT         2602 a         1536 c         1560 g         3660 t
ORIGIN      
        1 tttaaaatca gagccaccct caaaggtgaa agccctcttc ctgttgaggg gttttcgatt
       61 atatttgcgt gtatgaaagg atttattata aaagggtttg cttcaaaacg gattacttcg
      121 cgccttcctg cagttacaat taaccaattt tttaaaattt cacctgttgt caattcaata
      181 tccccccagt attcaaggat ttccaatctg ttgtcacaga ctgatttatc ggtttctttt
      241 cttgttttgt ctgccaccac tcccttcagt atttcgattt tgacttcgtc taaaagattg
      301 tttgctttgt ttgcacttat ttcttcaaca tcaaggtatg ttctgtaaat ctttgtgcat
      361 gaatcccaac tgtcacgcct gtttttatca aaaacgaagt cttcgggctt gatgtatttg
      421 accttgggat tgtcataaat aagctggttt tcaatcacaa atccgtcttg agttggtgcg
      481 ataagctgct cttcaatgct ttgtgcgcgt cttatgcttt taatttttgt ctcccaaccc
      541 acaaaaagag tgctttcgcc tgtttctaca attccgtcaa taactttttc caattcatct
      601 tcaagtttca tttgctcaaa agtgtttacc agcagtgcct tttgtctgtt ggcaaaagct
      661 tgtgactgag gggttacgcc cgatacatca aacatcgcat caggatgcga atatagattg
      721 tcgcttatat gcgactttaa ggtctgagca agctcgtaaa tgtcgggtaa ttctatgttt
      781 gaatcccaac cgttaatctg cggaacttga tttgtgtaaa tggcatttct gacaagtttt
      841 atgtcgtcaa gctggattct tctgtcatct tcaagttcgt cgtatttttg ataaattttt
      901 tgaacaagag ttttttcatc ttctgtcgga agctgaattg ttaaatcttc atctgttttt
      961 tcaaagttca tgttaatctc ctttttatta agtaaatagt aaattattaa gtaaatggta
     1021 aatggtaaat agtaaataga agtgaataag tgaataggta aataagtgaa taagttcaat
     1081 ttgttgcgaa gcaacaaaaa tattaacctt tcagctgttc acccattcag ccattaagcc
     1141 aatacaaccc tttcagcttt tcacctgttc acctgttaag ccatttacga ttgaatatac
     1201 ttctatatcc tgtaaaacac cgtttctaag cgtttctgct tttaaaagtg cttctttttt
     1261 aaagcccgag cgttttaaaa gtgctttgac cttgaaattt tgaggaaaaa ttgtcgcctt
     1321 aagttttttg agtttgtact ttttaaagca gtattttata aattttttgg cgcaaaattt
     1381 ggtgtaattt ccccagaaac tcgggttaaa gcaggttgta atctctgcag agtgaaaatt
     1441 gttttctgct ccgataaaat tgtctaaaaa agcaaatcct gcaactttat tgcttttttt
     1501 gtgaattaca acccaaaaat acggtgaagt tttttcaatg agatttaaaa tcaagtcaat
     1561 cgaatcttca tacttttgta taaaataatc gtcgtgaaga taatttgcaa atcttttgta
     1621 caaatttagc acttcgggta tatattctat gtttttaaac tcttttcggg ttttatttat
     1681 tattattttt ataaatttgc tcataaatac ttgacttttt aaaaatctga ctatacaatt
     1741 aactcagtga aaggaaggtg tttatatgaa ttctgaagaa attatgtttc aacaatatag
     1801 gctttatacc gagcaaaaag aaaagttcgt ggatagaagt tttcatacca ataaatttta
     1861 tcttttagtt gttttgggtt tgtttcttat aatgttttta ttaaagggat ttgcttttgc
     1921 ttttgatatt tctgctatat tggtctttag tgccgcaggt atggcaattt gcgttctctg
     1981 gtggataaat atggatgctt ataacttttt gattaaagtt aaattaggca aagttattga
     2041 agaaattgaa aaacaacttc catctcagcc ttatacgcaa gagtttcttg ctataaaaga
     2101 tttgagaaaa aataaaagaa tgtttttgtt tgcggatatg caaaaaatac ttgctatttt
     2161 tgcatttctg actttcctta ttttatttgt aaatgaagtt atacctttgt ttataataaa
     2221 gtaataaaga tttaatgcta aagtgaaggc tagcggtcgg gggaccgctt ttttttggtg
     2281 ctacgcacgt aggcactctg tacagattat ttaaaatact cttgaccaat gttttttgcc
     2341 tttatgcaaa gaaaaacgct gtaaatttat tttatactta ttgcgagcgg gaaaaagcaa
     2401 tgttacgcgg gcaaaaaagc ttatatttta catttctact ccctcaaatt ttacataagc
     2461 atcttgtgtc gaaaatgcat ctatttgtcc caatcgtatt ttatctctga gagaattttg
     2521 cagacccagc attgcctctg cttgaattcc gtttataaaa gtttgtgttt tgtttagccg
     2581 gttgaaaatt ttataaatag ctgctttgct gaaaattaaa tgaggtggaa cttttgtgat
     2641 atctgttatt aaattttttt ggggtttttg ttttgatatt tcaaattcgt gcttgatttc
     2701 ttcttccatt ttctttttta taagttcatc aagcgaaact tcgcttaaaa gcttttgttc
     2761 aatttctttt tcttcttttt tcattttttt tcctttatat tttgtcttcg tcgaggttgc
     2821 ttattgtaat tatttttgtc tcagaaggct cttcatcgga tttgtttgca aaccctaagt
     2881 gtttacaaag agattcaagt gctcttaagc ctgctgttgt gtctcttagt ttctttttac
     2941 ctgagggttg agcgtctttg tctagaatgt cttcttcttc aagtgaaaat tccactatat
     3001 ctaaaagctt tttgacaata tatccctttt ggattactag agaagagatt tgccttttaa
     3061 tttgattttt aatctcattt attattaatt tgtctgataa aagcttctgt gcaacatttt
     3121 tgtaatcttt agttttgtag cccgcgtttt ttgcggaaag ctccaaatcc agtgttttta
     3181 tgtattctgc gacaaatttt ttttgttgtt gtgtcaagtt tttcattttg atttacattt
     3241 cttattattg tttgtgtttt tttgaaaaaa attatataat agtcatgcta tttatatttc
     3301 ggctagtgta aaatcttaac agggagggaa aagttggatt aactgatatt tttggagttt
     3361 gttttgataa ttcctttcat cttgcaatct ctttttcggt taaaaaaact tatacttccc
     3421 tgttttttat ttggattaat cagtttcttg atagcttaat ttcaggtgtc tcaagagcat
     3481 ttgtgccgat ttttgagcgc atgttcgcaa gagcgtcttt atacatgcca taccagtaat
     3541 taaacttttt atgctcaggg tttgccttaa ctctcatgca ggttccatag accaaaagcg
     3601 gttccgcgta agcatctgga ataatagaag aatcttgctc caaagacatt ttgggaatgg
     3661 aggagccttg tgaatcttgg gcgtgaaaat tagtgtagta aattatttca acggtttttt
     3721 cctgattaaa aagcggaaat aaaagtttat cgttaaacgc actaaaggtt tttgacggtt
     3781 gtgtgtttaa aataaatttt tcaaaatcac cgcagtactc gtattttata ttgttgataa
     3841 aaaccgaatt tattcttccg ttaatcgtat tttctatttc cgggcaattt gcagggagtg
     3901 tcaaattggt ttttcttagt aaaaaactcc agttgtcaaa tccgcaaact tcggaattta
     3961 ttatgttaag tatattttta attttctcgt ggtcgttttt gcttaattcg gaaaagctcg
     4021 taacctttct gtaattaagt tctaccaggc atttgtttat taattcaaag taattcattt
     4081 tatctccttt taatcaagta aagtgtgaag cgtgaagggt gaaaaggttt atttctcttc
     4141 actcttcacc catcaccgtt cactttataa gtcctttttt aagttgttcc ataattaagg
     4201 gctcatattt agtaaattcc gcactgctca ttttgccgat ttgctcacgt gtaaaagttc
     4261 ttgatagatt tttgtcttgg aagtttgaat tttgggcatt tgccgtcaat ttttgctttg
     4321 caagttcatt tgaattttgt aagttctttt cgtaagaatc tttttgtaaa tatctttcga
     4381 ttgcggtttt ttcaatgttt tcaatcatat cggatatttt tttaagatcg tcttttccaa
     4441 ttattacagc atctgatttc aagtaatcca aaacttcttt tctgccctca ggctggaaga
     4501 aattaggatt gtttgtttca aaatcgctaa acacttcatc ttttttaaac gccggtgagg
     4561 ggttttgttt aatcttttca ttttgtaata ccaaatcaaa cgctttttta attatttggt
     4621 ttttcaaatt ttgtccttgc caagaattga ttaaaccgaa tttcataaga ctttctattt
     4681 tttgaaaatc ttgtgcaatt acattttcta cctctttttg aatgtgaaga ttttcctgat
     4741 ttttataaaa atcagtgtct tctcttcttg tttcaaccgg agccgacttt tcttgaatat
     4801 ctttattttc aggtttgatt tcaagttttt caagaacctg attgtaagtg tcagagtctt
     4861 tttgctcttt gccttccgct tgagaagatt tgatttcttt ctcagcgggt tttgcttgta
     4921 aattttggct ttcggcacaa gacgattctt tttcattttc tgtttggctc atttatgcct
     4981 ccaaattttt aatgtctttc atgtatttga ctgcctgttc gaccgcttcg tcaataaatt
     5041 ttgacaaaat tacaacaaca atacttttaa gcggctgaaa tataggcagc attgaaacta
     5101 tatattcaat agccatttgt tttttttctt ttccgctttg tgtgtcaagt gattgttccg
     5161 cgatgtgcac agccgagtag gcaagttctt ttatattgtt ttttatattt ttaaacattt
     5221 tttcctttct taaaaggtga gaagtgagct gtgaacagtg agggagtttt aatgccgttc
     5281 actgttcacc gttcactgtt caccgttcac tgttcacccg tttacactgc tgtaacaatc
     5341 atttttgcca aagatttagg cacaactgtt tttgctccgt acagataaag tcctcttaca
     5401 aggtcagaga agctgtcttt atctctaagg ctttcgattt ttgcaagttg ggaggcaaaa
     5461 gttattgctt cgtttgttcc tgccagaacg tagaatttac cgcctacggc agataaattt
     5521 gtgcttacaa gaacgtccat gcctgcaatt cttcctatcg caccttctct taatgtttcg
     5581 tctgcaacat tgtgagcact tataaattca gggctttgaa gcagataaga ttcgatgctc
     5641 gggttaatta caacccaagg tttttggttg tttgaaagtg catctgcatt tttgagttta
     5701 agtgcaagat ttacaaagtt ttcatagatt gttgttttgc ttaaagtaac agcactttgt
     5761 tctgttccta caacgtttga agaatcggca taagcatgtt gtgaaagtaa gtaggtgtct
     5821 tgaacctgtt caatcgcttt tttggcattt tgaagatgtg cttccataat gctctggtta
     5881 gcttgtgctt gtgcaacatc attaattttg aacgcaaaga attttttctg gtctattatt
     5941 aaatctgttg ttgaaggaga aagctcgttg taggttatag cagaagagct tactgttgaa
     6001 accgtaactt ctgcaggagt gattatttta acgcaatccc cttgattttt gatttcgcct
     6061 tcgtaatttc tgtttacgca ttgaagcata acacaatttt ttgatagcat tgtattcagc
     6121 ttttggctcc atatttcagg gataaaagct gaataagcgt ttactgtttg atcgctcatt
     6181 tttttgttcc tttctttttt taatgtaact tttatttttt aacagtgcgg aatttttatt
     6241 catctaaata tacttcttta aattcaagtc ctattattgc catatcatgc tcaatggaat
     6301 ctccttcaac gcagagttgc accgaatagt tggattctga aatttctgct ttataagttg
     6361 aatctatgtt tgcagaccaa aaagaaatat gctcggaatc atcatcccag taagaatttt
     6421 tttcagtgtt ggtttcactg tcccagacta aggttgttat gacttcggaa aatattatat
     6481 ccgcatcatc ttttgtctca gaatcgtaat ttttgtaggt ggcaaattta aaataattat
     6541 cataaccttc atctactaca aaataaaatt catcaattgt ttttcttgaa tttgtagggc
     6601 ctgcacataa aaaaggtgat ttccacataa attctattgc gtgtccgtca aaagtatttc
     6661 caaaatcttc tttatagata ttgccttctt tatctgcggt taaaatatcg tctttaaata
     6721 tgcaagctgt tgtaatatct tggggtaaaa tccttttaag ccaagctttg ttgacataat
     6781 cgttaatcca aatagtatga aaataatcat cgtttttata tggaataaaa taccaaacct
     6841 gatttttctt ttcataattc agggcgatta tttcgtcaaa acgttccgta tcaaattttt
     6901 caaattcgga ttttatattt agagtaattt catttcccaa ctgaatttga tttaattctc
     6961 ccacttgttc aagggcaaaa ataccttgat ttatgaaata ctgtttatta ttgacgttaa
     7021 taacgccgtt aactgccgcc gcgcctttat ttgcaaacgg aattatagtg aattcatcgg
     7081 gattggagcc ggtaagaaga taaaccatat tttttttgta aatggcaagg tagtctttat
     7141 atggtttaag ggcgattatc tcatcggtat cagtgtgaaa atcttttata aatccggcat
     7201 ctccttcggt cgtaaaatca tcgtatttgc ctagtgcaga aaaatatatt acagaatctt
     7261 ttgctgccca aactctgcct ttataaacag ctacaatgtt tgtaaaaaca ggaattcctt
     7321 cttcatctgt aagctggcag tcatatacat caaatgtttc attttttttc atataataaa
     7381 gggtatcgga tttgcttgaa ataattacac catttaaata atccgtaaaa ttaactgttt
     7441 tgctgtcttg aaattcaatg ggaatttttg caggttcttc tgattcaaaa tcaaatatac
     7501 aaaaagtacc tttttctgat gagcataaaa tgaaattttt tttattcatt ttaatttggt
     7561 gcagccctgt aattttttcg gaatttttat cttttgcaat taaaatattg cctttttgtc
     7621 ttaccagccc tttattttgc aaaatttcaa tattttttga atcgctccag tagagtttat
     7681 ttgtatcaag tcctaattct gttttggtgg aagattgttt aattcctcct ccaagattgt
     7741 aaaatgtatc tgtcattttt tttctccgat tttattcatt ttcaacgtac catctgattt
     7801 ttgatcttat aaagcttcct atatcatctt ttttaatcca gcaaaaaggc ggaagaaaga
     7861 tgatatcgat tttgcctttg cttgttgtat tgggatgtgc ctgcccgaat tcgtagtgtg
     7921 tcataacatt ttgggggcaa atgtctatat catatttttt tgccagttgt gcgcataatt
     7981 tcatagttgc ttcaaattga attcttgtta taggaaattg tccgaaattt tttgtatctt
     8041 taaaatttgc catagcacac attgcaactc ctattgagcc tgtgttgccg cctcctgtgt
     8101 gcggggcgta cattcctgtt ttacattcaa gatttgcttc tggcttgaat ttgcccaaat
     8161 ggattttacc taaagtgtca actaaaaagt gataacattc tttttcataa gaagtcggga
     8221 cgggcccgcc ggctgtccag tgtaggataa ttcttttcat ttttactcct taaacggtgc
     8281 tgaaatttaa gctgtagtgc ctgatataat tagctacagc atttgttctg tttctggcat
     8341 ttaatttaat aacacttcgc atgacgtgtg tttgaactgt tcttgcagaa atatttaaaa
     8401 tataagctat ttctttgtcg gaatagccaa agcttaacag ctcaaggatt tccaactctc
     8461 tgtttgataa cttcatcata attcctttta aaaagctact attcgatatt attcttaaca
     8521 ccggccagtt tatttcatgc tacatgctaa aaaacagtat attctaatgt attttctttt
     8581 tgttaaaaaa atacgagggg tttccccctc gagtagagta agatagtaaa gacaagttat
     8641 gtcttggtta ggatagtatg gaatatgtgt tggttatgat tggtttatgt aagggttgtt
     8701 ttcaaacaac cctattttaa gcgaacttaa atttgaataa catttctttt taactccaaa
     8761 ttcgttgtaa ttaagataaa attttgaaaa tactttaatg cttttatatt cgggctcttt
     8821 tttacataga attgaatttt gcaaattttc aaaaagtttt tttgcttttt cgttgacttt
     8881 tctcacaaat gaaatatcgt gattaaaatc tattctggta atttgtacta ccgtatgacc
     8941 acaagcagga cattttatta aataatcaag ctcaacaagc aaataatttt tttcgggtac
     9001 aagcgtataa cttttgcttt ttctttttgc cccgcagcag tgaatgtagc ccatgtaaac
     9061 ccttatttaa acactattat cccctgtttt atggcaaaca gctttaccat cgagggtgtg
     9121 agccttaacc acataatcag tataaatcat tttcaaattt aaaaaagtcc gcaaaactac
     9181 gtagtacatg ccgtgtacgt atttttacgg acaaaaaata aataaaagag gaggcttaaa
     9241 atatttgaca accttaaaat taaggcttaa aatcattata aaaagagagg cttaaaataa
     9301 tgaaaaataa aatattaaaa aggatttttg ggtttacatt ggctgaagtt ttgataac
//
LOCUS       DAOQAU010000061         9013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_9150_length_9013_cov_6.358227, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000061 DAOQAU010000000
VERSION     DAOQAU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9150_length_9013_cov_6.358227"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /gene="fusA"
                     /locus_tag="PKI94_07755"
     CDS             complement(<1..1745)
                     /gene="fusA"
                     /locus_tag="PKI94_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNW26665.1"
                     /translation="MARQTPLERIRNIGIAAHIDAGKTTTTERILFYTGKIHKIGEVH
                     DGAAVTDWMEQERERGITITSAAVTSFWKNHQINIIDTPGHVDFTIEVERSLRVLDGV
                     VAVFCAVGGVQSQSETVWRQANRYEVPRMVFVNKMDRVGANFYRVVDQVKSRLGGNAH
                     PIQLPIGAEDQMKGLIDLVEMKAHIYTNDLGTDIKIEEIPADMLEKAKEYRLELIEAV
                     SEFDDDLMMKFMEGEEPTIEEIKAGIRKAVCENKIVPVTCGTAFKNKGVQLLLDAVVD
                     YMPAPTDVKAIQGELEDGTKVERHSSDDEPFSALAFKVMTDPYVGRLTFMRVYSGKLS
                     SGSYVINSTNGNKERISRLVQMYADQRNEISEVYAGDICAAVGLKDTTTGDTLCDEKN
                     PVILEKMTFPTPVISVAIEPKTKADQDKMGIALQKLAEEDPSFQVKTDQETGQCIISG
                     MGELHLEIIVDRMLREFKVDANVGKPQVAYRETIRGTADQDSKFIRQSGGRGQYGHVK
                     VRIEPAEENAGFVFENKIVGGSIPREYIEPARKGMEEALEGGILAGFPMIDVKVTLYD
                     GTYHDVDSSEMAFKIA"
     assembly_gap    1846..1945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2055..3620
                     /gene="purH"
                     /locus_tag="PKI94_07760"
     CDS             2055..3620
                     /gene="purH"
                     /locus_tag="PKI94_07760"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNW26666.1"
                     /translation="MKKRAYISVFDKDGIVELAQNLIKNDFEIVSTGGTFKFLKENGI
                     KVIEVSKVTGYPEMLHGKVKSLHPEIFGGILADSTNSDEAEEIKINDVKKFDLVVVNL
                     YPFEQVAMQTNDINKLVENIDIGGIALLRAAAKNYKNVTAICDKKDYGRAASAPEETR
                     QELALKAFDLTSNYDTIIGAKLREVFEHKEIKHFNFEKIQDLRYGENPHQKAGLYKIN
                     DGGIAIPPYANKSRAELPFRRQEVNYEILWGKELSYNNILDMTAAVNIVSEFYDVPAA
                     AIVKHTMPCGVALGRDLNDAYQKAFDCDPISAFGGIVAFSQGVNEEIAKHLNSVFLEV
                     VIAPDFDDNALEILKSKKNLRIVKLNTKLEEYKLLQNEEIKITPFGVLIQEQDNKELD
                     KDTFKVVTKTKPTAEQIEDAIFAWKVAKHAKSNAIVIVKDFKTLAIGQGQTSRICAME
                     WALNYACDGTKDAVVASDGFLPAVDNIQAAVQGRIGLIIQPGGSIKDNEVIAEADKYN
                     LAMITTGIRHFKH"
     gene            3725..4903
                     /locus_tag="PKI94_07765"
     CDS             3725..4903
                     /locus_tag="PKI94_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW26667.1"
                     /translation="MNTIQKNKRAMGWLTTAHLANDMYAGFLNPIMPFLAAKMGFTIA
                     IATLIMSVAQVCASLIQPIFGFFADNILKRVFIFWGLIFGSLFIPLGTNAPNVFLLTV
                     CVILGNLGGSFFHPQALGFISRFSDRNFVQNMGIFITAGTLGFALGPIISGSVVQHFG
                     FEKIPYLSIFGIVIALLMFSFVPKISDKNIVLKHKEFKKSFSDILSNKNMLILINVSV
                     AKTLMANSCTILLPFLWKDMGYSPSYIGFALFGFLLAGGFGSILSSRIEEKIGSKTVF
                     YISLIATLPLTGLFVLSYRPYPNVAFAIFVLMGFFAMLAQPVMLVMAQRILPDYKSIV
                     SGFINGFSWGIVAVALTLVGFFAQKFGIAKVVFIVSFLPVLFSYFVRFLPDKVEPSST
                     "
     gene            complement(4896..6017)
                     /locus_tag="PKI94_07770"
     CDS             complement(4896..6017)
                     /locus_tag="PKI94_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26668.1"
                     /translation="MNKEDLIIGIHGDGGFTGGHYNVIAGFSTGLLRGFQNCGVKAYS
                     TKECYEKRLLPNLTIGFNVSGFEAWEEYLKRNIINIMWNVDSIFFQNIEAVKQFQANP
                     NFFLFNISPSDTEALQEYFPLLKHAYVPHAVDLELWKKKDLEKEYDIVFLSSIFDWEA
                     KIEELRESLPKQTFELLMIMFETWMNTPNISFWQMYQIFKQEANLVLDVDQYNFMFKN
                     LSYLVSFAKRAQVIEKLQNFNVKIFGSGPWHKYTKGKVQYMGECDLLESVDVMNKAKI
                     VLHVHPSQLTLGLHERILNASAVESFVMSSDTASIQSEFGDSMGYFNLTNFDDIEEKM
                     AYFLNNPFEMEQKAQIAKEITEQKHTWTKRAEAILEIIK"
     gene            6118..7290
                     /gene="alr"
                     /locus_tag="PKI94_07775"
     CDS             6118..7290
                     /gene="alr"
                     /locus_tag="PKI94_07775"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNW26669.1"
                     /translation="MKTSRQSYINVHRDSWVEVNLEYLAQNVKQIKKLIQNGVKLLAV
                     VKADAYGHGALMVAPTMLASGVDVLGVASIDEGLNLRDAKINCEILVLGAVPIWAFES
                     AVENDIIISIFSDEHINACRQAFVRTGKKVKAHIKVDTGMNRIGVGEADAEEFIKKVQ
                     AAEFIDLKGIFSHLACAEDERETKQQFEKFKKIISSVDTKELDVHILNTAGIISYPDY
                     QFNMVRAGIGLYGLMPTLSKETVNVIANKNESIQKYSSPFHPFTLSPIMSLKGRISRI
                     HTMGANEGISYGHTFVANKEVIVATVPIGYADGVPRALSNKIFGEINGKKVKQIGNIT
                     MDQMMFDISGIKANEGDVITLLGEGLTIDEWAKIVGTINYELTCRLKVRLPRVYTR"
     gene            7343..8269
                     /locus_tag="PKI94_07780"
     CDS             7343..8269
                     /locus_tag="PKI94_07780"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNW26670.1"
                     /translation="MRLPDYLKRPIIDTEKTKKVRKVLKECCLNTVCESARCPNKNEC
                     YQKNTATFLIMGNFCTRNCRFCNISCEKPLPLDKNEPKNVAKAAKELGLNYVVITSVT
                     RDDLPDGGAEHFVKCINEIKKNVPPSRGNRNLLISKGGITPHPTPPPQGGRENVPKIE
                     ILTPDFKGDKKALDTIIKSPVDVFNHNIETVPRLYKNARPQADYQRSLDVLKYVKENS
                     KIPIKTGLMVGLGETFEEIEQVLVDLKSIGIDILTIGQYIAPSKEHLAVNEYYSLEKF
                     EQLKRLTEKIGIKNYQIGPLVRSSYNAFEIFR"
     gene            complement(8396..>9013)
                     /locus_tag="PKI94_07785"
     CDS             complement(8396..>9013)
                     /locus_tag="PKI94_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNW26671.1"
                     /translation="WTIGLCEAIIESGLKITWSSNTRADTADDEMAELMYDSGCRLVS
                     IGVESGSQFILDKIGKKVTIDDIRDTVKIFKRAKIKTYNYFVIGLPWDDEDTIEQTIN
                     FAIELDSDFVSFYTATPLPGTRFFRYAKEHNLLEKQNSWANAYYEPVVKTHCLSKERI
                     MELHKQAVKRFYLRPMYILRTIINIRSFAEIKNYFLAGIGLLLRK"
BASE COUNT         2764 a         1642 c         1668 g         2839 t
ORIGIN      
        1 gcgattttga acgccatctc agaagagtca acatcgtggt aagttccgtc atacagagtg
       61 actttaacgt caatcatcgg gaatcctgct aagataccgc cttcaagggc ttcttccata
      121 ccttttcttg caggttcaat atattctcta gggattgaac cgccgacgat tttgttttcg
      181 aatacaaatc ccgcattttc ttccgcaggt tcgattctga ctttaacgtg tccatattga
      241 ccgcgaccgc ctgattgcct gataaattta gaatcttgat ccgcagttcc tctgatagtt
      301 tcacgatatg caacctgagg tttaccaacg ttagcgtcaa ctttaaattc tctgagcatc
      361 ctgtcaacga taatttcaag gtgaagctcg cccatccctg agataatgca ctgacctgtt
      421 tcctggtctg ttttaacttg aaaagaagga tcttcttcag caagtttttg aagcgcgata
      481 cccattttat cctggtcggc ttttgttttt ggttcaatcg caaccgagat aaccggcgta
      541 gggaatgtca ttttttccaa aattacaggg tttttctcat cacagagagt atctcctgtt
      601 gttgtatctt tcaatcccac agccgcacat atgtcgcccg cgtaaacttc agaaatttcg
      661 tttctttgat cggcatacat ttgaactaat cttgaaatac gttctttgtt tccgtttgtt
      721 gaatttataa cgtaagagcc cgaagataat ttacctgaat aaacacgcat aaatgtcaat
      781 cttcctacgt aagggtctgt cataacttta aaagcaagtg ccgaaaacgg ttcgtcgtca
      841 gatgagtgtc tttcgacttt tgttccgtct tcaagttcac cttgaatagc tttaacgtca
      901 gttggtgcgg gcatatagtc aacaacagca tccagcaata gctgaacacc tttgttttta
      961 aatgccgtac cgcaagttac aggaacaatt ttgttttcgc aaaccgcttt tctgattcct
     1021 gcttttattt cttctatagt aggctcttcg ccttccataa atttcatcat taaatcatca
     1081 tcaaattcag aaactgcttc aataagttcc aacctatatt cttttgcttt ttcaagcata
     1141 tcggcaggaa tttcttctat tttaatatcg gttcccaaat cgtttgtata aatatgtgct
     1201 ttcatttcaa caaggtcgat taagcctttc atctgatcct cagctcctat tggaagctga
     1261 atcggatgag catttccgcc gagtcttgat ttaacttgat caacaacccg atagaaattt
     1321 gcacctactc tgtccatttt gttaacaaaa accatacgag gaacttcata tctgttagcc
     1381 tgtctccaaa ctgtttctga ttgtgattga acaccaccta ctgcgcaaaa tactgctaca
     1441 acgccgtcta atacacgcaa tgaacgctca acttcgattg taaaatcaac gtgtcccggg
     1501 gtgtcgataa tgttgatttg gtgattcttc caaaaagatg taacggctgc tgaggtaatt
     1561 gtaattcctc tttcgcgttc ttgttccatc caatcggtta cggcagctcc gtcgtgaact
     1621 tcgccgattt tatgaatttt gcctgtgtaa aacaaaatac gttctgttgt tgtagttttg
     1681 cccgcatcaa tgtgcgcggc tataccgatg tttctaatcc tctctaaggg ggtctgtctt
     1741 gccattgttt gtttgctcct ttattctaac ttttctgttt tttgtcctag taaataggaa
     1801 atagtaaata gtgaatagaa aatgaaaatc tatttaccat ttactnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnctatt taccatttac ttttatattg tcgcacaaac
     1981 aagcaaattt tcaaatatac gtttaatttt ttttaatatt tatgctagta taagtgagaa
     2041 ataaggagca aaaaatgaaa aaaagagcct atataagcgt tttcgataaa gacggtatcg
     2101 ttgaactggc gcaaaatctt attaaaaatg attttgagat tgtttccaca ggcggaactt
     2161 ttaaattctt aaaagaaaac ggaataaaag ttattgaagt atctaaagtt acaggctatc
     2221 ctgaaatgct gcacggaaaa gtaaaatcgc ttcatcctga aatatttgga ggcatccttg
     2281 ccgattctac aaactcggat gaagcagaag aaatcaaaat caatgacgtc aaaaaatttg
     2341 atttagttgt ggtaaattta tatccgtttg aacaagttgc aatgcaaaca aacgatataa
     2401 ataaacttgt tgaaaatatc gatatcggag gcattgcact tttgcgcgcg gcggctaaaa
     2461 attataaaaa tgtaaccgca atttgtgaca aaaaagatta cgggcgcgcc gcaagcgcac
     2521 ccgaagaaac acgccaagaa cttgcgctga aagcttttga cttgacttcg aattatgata
     2581 caataatcgg tgcaaaatta agagaagttt ttgaacataa agaaattaag cattttaatt
     2641 ttgaaaaaat tcaggattta agatacggcg aaaacccaca tcaaaaggcc ggattataca
     2701 aaattaatga tggtgggata gcaatcccac cctacgctaa caaaagtagg gcggaattgc
     2761 cattccgccg tcaagaggtc aactacgaaa tcctctgggg caaagaatta tcttacaaca
     2821 acattttaga tatgacagct gcggtcaata tagtaagcga attttatgat gtgcccgccg
     2881 ctgcaatagt taaacacaca atgccctgcg gggttgcact tggcagagat ttgaatgatg
     2941 cttatcaaaa agcctttgac tgtgatccga taagtgcctt tggcggaatt gtcgcttttt
     3001 ctcaaggggt aaatgaagaa atagccaaac atttaaattc tgtttttctt gaagttgtta
     3061 tcgcacctga ttttgatgat aatgcccttg aaatattaaa aagtaaaaag aatttgagga
     3121 ttgttaaatt aaacacgaag cttgaagaat ataaattatt gcaaaatgaa gagattaaaa
     3181 taactccgtt tggagtttta attcaagaac aggacaacaa agagcttgat aaagacactt
     3241 ttaaggtggt tacaaaaaca aaacctacgg ccgagcagat tgaagacgcc atttttgcgt
     3301 ggaaagtggc aaaacacgca aaatcaaatg cgattgtaat tgtaaaagat tttaaaacgc
     3361 ttgcaatagg acaaggtcag acaagcagaa tttgtgctat ggaatgggct ttgaattatg
     3421 catgtgacgg gacaaaagat gctgttgttg cctctgacgg atttttacct gctgtcgata
     3481 atattcaggc ggcggttcaa ggcagaatag ggcttataat ccaacccggc ggaagcataa
     3541 aagacaatga agttatagca gaagccgaca aatacaattt ggcgatgata acaacaggta
     3601 taagacattt taagcattag aggtaaatga gcaaaaagtt gattaattga atagttaaag
     3661 ggcgggagtt gcaaagcaac tcccgccctt tttcgttctt ttattttcaa agtataatat
     3721 tcttatgaac acgattcaaa aaaacaaaag agcgatgggg tggttgacaa cggctcatct
     3781 tgcaaacgac atgtatgctg ggtttttaaa cccgattatg ccttttcttg ccgcaaaaat
     3841 gggatttacc atagccattg caacgttgat tatgagtgtt gctcaagttt gcgcctcttt
     3901 aattcagcct attttcggat tttttgcaga taatatttta aaaagagtgt ttattttttg
     3961 ggggttaatt ttcggctcgc tttttatccc tttgggaaca aacgccccga acgtttttct
     4021 tctcaccgtt tgtgtgattt taggtaatct tggcggaagc ttttttcacc cgcaggcgtt
     4081 ggggtttatt tcaagatttt ctgacagaaa ttttgttcag aatatgggga tttttattac
     4141 tgcaggtaca ttaggctttg cgctcggacc gattatttca ggcagcgtag tacaacattt
     4201 cgggtttgaa aaaatacctt atctttcgat ttttggcatt gttattgcgc ttttaatgtt
     4261 ttcttttgta ccgaaaattt ccgataaaaa cattgtcttg aagcacaaag aatttaaaaa
     4321 atctttttcg gacattttgt caaacaaaaa tatgcttatt ttaattaacg tttctgtagc
     4381 aaaaacttta atggcaaaca gttgcaccat attgcttccg tttttgtgga aggatatggg
     4441 atacagccct tcttatatag ggtttgccct gttcggattt cttcttgcgg gaggttttgg
     4501 ctctatttta agcagtcgta tagaagaaaa aatcggttct aaaacagttt tttacatttc
     4561 attgattgcc actttgcctt taacagggct ttttgttctt tcatacagac cttatcctaa
     4621 tgttgctttt gcgatttttg ttttaatggg cttttttgca atgctggcac aacccgtaat
     4681 gcttgtaatg gctcaaagga ttttgcctga ttataaaagt atagtgtcgg gatttataaa
     4741 cggattcagc tggggaattg tcgctgtcgc tctgactctg gtcggatttt ttgcacaaaa
     4801 attcggcatt gcaaaagttg tttttatagt ttcgtttttg cctgtgcttt tctcttattt
     4861 tgtgaggttt ttacccgata aagtcgagcc ttcttctact taataatttc taaaatcgct
     4921 tcggcgcgtt ttgtccaggt atgtttttgt tcggtaattt cttttgcaat ttgggctttt
     4981 tgctccattt caaaagggtt attaaggaaa taagccattt tttcttcaat atcatcaaaa
     5041 tttgtaaggt taaaatatcc catagaatca ccgaattcgc tctgaattga agctgtatca
     5101 gaagacataa caaaactttc gaccgcactt gcatttaaaa tcctttcatg caaaccgagc
     5161 gttaattgcg aaggatgaac gtgaagcacg atttttgctt tattcattac atcgacactt
     5221 tcaagcaagt cgcactctcc catatactga accttgccct ttgtatattt gtgccaaggt
     5281 ccgctcccaa aaatttttac gttgaagttt tgaagctttt caattacctg cgcccttttt
     5341 gcaaacgaaa caaggtaaga taaatttttg aacataaaat tatactgatc aacatccaaa
     5401 accaaattgg cttcttgttt gaaaatctga tacatttgcc aaaaagatat gttcggcgta
     5461 ttcatccaag tttcaaacat tatcataaga agttcaaaag tttgtttcgg caaagattct
     5521 cttaattctt cgattttagc ttcccagtca aaaatcgaag aaagaaaaac tatatcgtat
     5581 tctttttcta aatctttctt tttccaaagt tccaaatcaa cagcatgagg gacataggca
     5641 tgttttagca gaggaaaata ttcctgtagt gcttctgtgt cactgggaga gatattaaat
     5701 aaaaagaaat ttggattagc ttgaaattgt tttactgcct caatattctg aaaaaatatt
     5761 gaatcaacat tccacataat attgataatg ttgcgtttta aatattcttc ccaagcttca
     5821 aaaccagaaa cattaaatcc tattgtaaga tttggcaaaa gtcttttttc ataacattct
     5881 tttgtagaat atgctttgac accgcaattt tgaaagcctc ttaacaatcc tgtcgaaaaa
     5941 ccggctataa cattgtaatg accacctgta aagcctccat cgccgtgtat tccgattatc
     6001 aaatcttctt tattcataaa acttcccctc tgagtttgca aaaattttta atttttctgc
     6061 atcctctctt ttgtttcatt ttagcatact atttttgttt agcatataat taaatttatg
     6121 aaaacatcaa gacaatcata cataaacgtt cacagagatt cctgggttga agtaaatctt
     6181 gaatatttag ctcaaaacgt taagcagata aaaaagctta tccaaaacgg agttaaactt
     6241 cttgcagttg ttaaagcaga cgcttacgga cacggggctt taatggtcgc acccacaatg
     6301 ctcgcctcag gggttgatgt tctgggcgtg gcttcgattg acgaaggctt gaatcttcgc
     6361 gacgccaaaa taaattgtga aattttggtt ttaggcgcag ttccgatttg ggcttttgaa
     6421 agtgctgttg aaaacgatat tataatttcg attttctctg atgagcacat taacgcttgc
     6481 aggcaggcat ttgtaagaac aggcaaaaaa gttaaagcac atattaaagt cgataccggc
     6541 atgaatagaa tcggcgtcgg cgaagccgac gccgaagaat ttattaaaaa agttcaagca
     6601 gctgagttta ttgatttaaa aggaattttt tcgcatctag catgtgccga agatgaaaga
     6661 gaaactaaac aacagtttga aaaatttaaa aaaataattt cttctgttga cacaaaagaa
     6721 cttgacgttc atattttgaa cacagcgggg ataatttcat atcctgatta tcagtttaat
     6781 atggtaaggg ctggaatcgg gctttacggg ttgatgccga cattatcaaa agaaacagta
     6841 aatgttattg cgaacaaaaa tgaatcaatc caaaaatact cttcaccctt tcaccctttc
     6901 accctttcac ccataatgtc cttaaaaggc agaatttcaa ggattcatac aatgggggca
     6961 aatgaaggaa taagctacgg tcatactttt gttgcgaata aagaagtaat cgttgcaact
     7021 gtcccgatag gctacgctga cggtgttcca agagctttat caaacaagat ttttggtgaa
     7081 attaacggca aaaaagttaa acaaatcgga aatatcacaa tggatcagat gatgtttgat
     7141 ataagcggaa taaaagcaaa cgaaggcgat gtaataacgc ttttgggaga gggtttaact
     7201 atcgacgagt gggcaaaaat tgtaggtacg attaattacg aattaacttg cagattgaaa
     7261 gtacgtcttc caagagttta cacgaggtaa aaacatatgt cattctgaac ttgtttcaga
     7321 atctcataaa tgaggttttg cattgagatt acctgattac ttaaaacgtc ctataattga
     7381 taccgaaaag acaaaaaaag tcaggaaggt tttaaaagaa tgctgtctta atactgtttg
     7441 cgaatcggca cgatgtccca ataaaaatga gtgctatcaa aaaaacacgg cgactttttt
     7501 gattatggga aatttctgca caagaaactg ccgcttttgc aatatttcct gcgaaaagcc
     7561 tttaccttta gataaaaatg agccgaaaaa tgttgcaaaa gcggcaaaag aattgggttt
     7621 gaattatgta gtaataactt cggttacaag agatgacctg cccgatggcg gagcagaaca
     7681 ttttgttaaa tgcattaacg aaattaaaaa aaatgttccc ccctcgaggg ggaaccgaaa
     7741 tctattgatt tcgaaggggg gaatcacccc ccaccccacc cctccccctc agggggggag
     7801 ggagaatgtg ccaaaaatag aaattttaac tcccgatttt aaaggcgata aaaaagcact
     7861 tgacacaata ataaaaagcc ctgtagatgt ttttaaccac aacatagaaa cagttccgag
     7921 attgtacaaa aatgcccgcc cgcaggctga ttatcaaagg tcactggatg tgcttaaata
     7981 tgtaaaagaa aattcaaaaa ttcctataaa aacaggctta atggtgggtt tgggcgaaac
     8041 ttttgaagaa attgaacagg ttttggttga tttaaaatca atcgggattg atattttaac
     8101 catagggcag tacattgcac cttctaaaga gcatttggcg gtaaatgaat attattcttt
     8161 agaaaaattt gaacaattaa aaagattaac tgaaaaaatc ggaataaaaa attatcaaat
     8221 cggacctttg gtaagaagtt cctacaatgc ttttgagata tttagatgat gaatatacaa
     8281 tcgaattatt gcaagacctc atatacttaa cgtcatcctg aatttatttc aggatctatc
     8341 cagcatacta tgttgataag atgctgaaac aagttcagca tgacactagg gtaccctatt
     8401 ttcttaaaag tagcccgatt cctgctaaaa agtaattttt aatttcagca aatgagcgaa
     8461 tatttatgat agttcgcaaa atatacatcg ggcgaaggta aaaccttttt accgcctgct
     8521 tgtgtaattc cataattctt tctttgctta agcagtgggt tttaacaacg ggttcatagt
     8581 aggcatttgc ccatgaattt tgtttttcaa gaagattatg ttcttttgcg tatctgaaaa
     8641 atcttgtccc tggaagcggc gttgctgtat aaaaacttac aaaatcgctg tccagttcaa
     8701 tggcaaaatt tattgtctgc tcaatggtat cttcatcatc ccaaggaagc cctatgacaa
     8761 agtagttata tgttttgatt tttgctcttt taaagatttt aacagtatct ctgatatcat
     8821 caatggtaac ttttttacct attttatcaa ggatgaattg cgagccgctt tcaacgccga
     8881 tactaacaag cctgcagccg gagtcgtaca ttaattctgc catttcatcg tctgcggtat
     8941 ctgctcttgt attcgacgac catgttattt taagcccgct ttcaataatc gcctcacaaa
     9001 gtccgattgt cca
//
LOCUS       DAOQAU010000062         8744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_9553_length_8744_cov_5.798941, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000062 DAOQAU010000000
VERSION     DAOQAU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9553_length_8744_cov_5.798941"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKI94_07790"
     CDS             complement(<1..667)
                     /locus_tag="PKI94_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNW26672.1"
                     /translation="MRYRENVRNFCIIAHIDHGKSTLADRLLEFTGTIAERDMQNQLL
                     DTMDIERERGITIKLNAARMNYKAKNGKDYVLNLIDTPGHVDFSYEVSRSLAACEGAL
                     LIVDATQGVEAQTLANVYLAIEQNLEIIPVINKIDLPSADVERVRHEIEEILGIDASL
                     AIPVSAKAGIGIEEVLEAVVDLIPPPVDTSDKPLRALVFDSAYDQYLGTLCFFKIMDG
                     KLKL"
     gene            complement(957..2588)
                     /locus_tag="PKI94_07795"
     CDS             complement(957..2588)
                     /locus_tag="PKI94_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW26673.1"
                     /translation="MLSFNIISNQNKILPSSKSKNGFHFIKSKSHKNCEDKISFGKST
                     KAEDKNSFNTDSVSIYIKDISKLSRKSKEEINRLIFQAQGKGNTAENARNQVIENYLP
                     LVVSVAKTITKENNDFLDLVQAGNLKLIDLINAYKPEKGDFTIYAHCQLRYYMLSCLK
                     QNKNIISIPKNDNDKISMIKREIENFYSEKFRQPNENELAQRLKMSKERLSKLLPFAT
                     PLLYMDEPVREGSSKTFASTLPENEQEDCFKIFNPEISKNDLEFAINQLTPKQRFALN
                     SFFKENELTKDAENLGLEETGRITDAKEKALRKIRKILGNLKTKNEESKTFENLNPAE
                     ISGSFSEKNDYPFEMFPAEQAIKANGKKIKFTTIQYRTLNLLAQNSGKVVSYETFFWE
                     IWRENFDPKAKKLITMIKRINEEISSASKGEFEIISVKNKGYMLATEKTSFKIHDITF
                     FPELSSIKIGDKKTKLTELEYKMMLLFAKNEEKTIPHKEILESVWGEIPKNGVESISI
                     KLFTLRPKLKEISNGKMDIINVPEKGYKLTSSNKT"
     gene            complement(2654..3349)
                     /locus_tag="PKI94_07800"
     CDS             complement(2654..3349)
                     /locus_tag="PKI94_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW26674.1"
                     /translation="MNKILVIDDDKNINELVKINLELAGHKVIQALDGIKGFALAKQE
                     LPNAIVLDVMMPEVDGYTVAQRIRQNPDTKHIPILMLTALSQLNDKVKGFDIGVDDYL
                     VKPFEMEELRVRIRALLKRTNQIPESAATKEILTLNEITLLPEIYSVKINEKQAKLTP
                     IEFEIFHLLMQNHGTMVSSAKLLSDIWGYAPDDDVETIRVHIRHLRTKIDKIANGKKY
                     IETIYGGGYKLNV"
     gene            3578..4021
                     /gene="speD"
                     /locus_tag="PKI94_07805"
     CDS             3578..4021
                     /gene="speD"
                     /locus_tag="PKI94_07805"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNW26675.1"
                     /translation="MAVTSPFSSINQNGKTQIHLGQHVLAEFFECDPNILNNVALVEK
                     LMVDAALECGATIVQKCFHMFNPYGVSGVVIIAESHLAIHTWPELGYAAVDLFTCGDK
                     CDPKVSYNFLKEKFNSKNASYKELKRGIIDEETMKVAHSPFELAI"
     gene            complement(4085..4372)
                     /locus_tag="PKI94_07810"
     CDS             complement(4085..4372)
                     /locus_tag="PKI94_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26676.1"
                     /translation="MILEYKVDETNVQAAWLKTLYDLVEELNCKCKTYIDESYSYTYR
                     HFSRTRIIEVYGSDTMLGWLKLRMERYLHFISAYNESGNIRAEMHDSIAQE"
     gene            4504..4845
                     /locus_tag="PKI94_07815"
     CDS             4504..4845
                     /locus_tag="PKI94_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNW26677.1"
                     /translation="MKVYNSSNNTVIAERAEVAKSFFSRTVGLILKKTLKEGEGLVIK
                     PCFSIHTFFMKFAIDVLFVNKKNEIVALYENVKPWRILPVHFNSYYVVELPSGTISST
                     NISKGDSIKFD"
     gene            5547..>6524
                     /gene="uvrC"
                     /locus_tag="PKI94_07820"
     CDS             5547..>6524
                     /gene="uvrC"
                     /locus_tag="PKI94_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNW26678.1"
                     /translation="MNKILSEKLKLLPSLPGCYLYSDIKGEIIYVGKAKSLKNRVKSY
                     FNKTLEGAKLNVLVPQIENLEYIVTNSEAEALILESHLIKKHKPKYNILLKDDKKYPY
                     FLITDEDFPRIIVTRKRNMNLEKGKYYGPYTDARAMYATLDFLKKIFPLKQCKQPKFK
                     SRPCLYYQIGRCLAPCQGMVTSEEYKKLIEQAELFLQGKQSELIKYLKNQIQEYSDSQ
                     QYEKAARLRDSYIDLQKTLERQRVVFENTKLNQDVISLIYDDGIFAIVILMIREGRLI
                     DKKDFVYFVELPLTPTPSPARGEGNSHGIETFTPSPARGEGNSHGIETFT"
     assembly_gap    6525..6624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6625..7548
                     /locus_tag="PKI94_07825"
     CDS             <6625..7548
                     /locus_tag="PKI94_07825"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26679.1"
                     /translation="PSPARGEGNGQNMVVGLKDFMETFFREYYGNLLLEFPDTIVSNQ
                     LELLGEKTLYQDWLELLAKKKVKISYAKSTQGQELQKLADKNAKVLLGNAKIEKMSKI
                     RDDFNEIGSFLAEKLKLKNFPHRIECYDISHIQGTKTVASMVTFINGVSKKSAYKKFK
                     IKLAEGKPDDFLSMKEVLTRRLKRLGDEGWEKPDLIIIDGGKGQLSAVMDIVNDFSVS
                     SLRGACDEAIQKNNADKILKQVQDDVTLGIDIVSIAKREEEIFLPNQSKPVILPRNSS
                     ALFLFQRIRDEAHRFAITYHKKLRERAAVKK"
     gene            7596..>8744
                     /locus_tag="PKI94_07830"
     CDS             7596..>8744
                     /locus_tag="PKI94_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNW26680.1"
                     /translation="MEFIQNFVHANSILVSVAILLLAYFFIATEKVPKVTIALAGATI
                     AILLGPLSQEKGMQGELNPAYFINYIDFNVIFLLVSMMLIVNIAARSGMFNWFATELL
                     RLTKGHPKSVLFVLGVFTAIASAFLDNVTTVILVMPVTFVVARELDITPVPFLITEIL
                     TSNIGGTATLIGDPPNIIIGSAAGLSFLDFLLELTDIVLIILLISLAILVFMFRKVLK
                     SSPEKMEHIAKLDNSGTIKDYNLMIRSSIVLFLVVLGFVLHDVIHVQSCIVAMAGASF
                     LLMFEKPHQILHDVEWNTIFFFIGLFIIIGGVEASGGISLMAQWLLDITNGSQKVAAM
                     VILWASGILSGIIDNIPYTVTMAPMLLEVQKAMGEAYIHPLWWCLSLGA"
BASE COUNT         2622 a         1445 c         1573 g         3004 t
ORIGIN      
        1 cgagttttaa ttttccgtcc attatcttga aaaaacaaag tgtgcctaaa tattggtcgt
       61 atgcactgtc gaaaacaagt gctctaagag gtttgtctga agtatcaacc ggtggaggaa
      121 taagatcaac aactgcttca agtacttctt caattcctat tcccgctttc gcgcttacag
      181 gaattgcaag gcttgcatca attcccaaaa tttcttcaat ttcgtgcctg actctttcta
      241 catcagcgga tggcaggtca attttgttta taacaggaat aatttctaaa ttttgttcaa
      301 tcgccaaata aacattggca agtgtttgtg cctcaacgcc ctgagtagca tcaacaataa
      361 gcagtgcacc ctcgcatgct gcaagcgagc gcgaaacttc ataagaaaaa tcaacgtgtc
      421 cgggtgtgtc aatcaaattg agaacatagt ccttgccgtt ttttgccttg taattcatcc
      481 ttgcagcgtt taattttatt gtaattccgc gttcacgttc aatatccatg gtatctaaaa
      541 gctgattttg catatcacgt tcggctattg tacctgtgaa ttcaagcaaa cggtcggcaa
      601 gtgttgattt tccgtggtca atgtgtgcta ttatacaaaa atttcgtacg ttttctctat
      661 acctcatgtc tgtcattata acctgctgaa gccaaaactt gcaacttttt aaaagtcgat
      721 taatttaaat gtttatttaa ttgtaacaag atgtaacaaa tatataacta aattatataa
      781 gatatagcaa ttttatatac caaaaagttt ttatataagt atagagtata aacagctaaa
      841 aactgcacga gaaaaagaga atattagaga aaaaatttta acacatagca cactacacac
      901 acttcacact gattaggaat ttattccgga gacccccacc acaaggtctc ttttttttat
      961 gttttgtttg aagatgtaag tttatagcct ttttccggaa catttattat atccattttg
     1021 ccgttagaaa tttctttaag ttttggcctc agcgtgaata attttattga tattgattcc
     1081 actccgtttt taggaatttc tccccaaacg gattcaagaa tttctttatg cgggattgtt
     1141 ttttcttcgt tttttgcaaa tagcagcatc attttgtatt caagttctgt caatttagtt
     1201 tttttgtcac ctatttttat agaagaaagt tcagggaaga aagtaatgtc atgaatttta
     1261 aagcttgttt tttctgtagc aagcatatat cctttgtttt taacgctgat tatttcaaat
     1321 tctcccttag aagcagaact gatttcttcg ttaattcttt taatcatagt tattaatttt
     1381 ttagctttag gatcaaagtt ttctctccag atttcccaaa agaatgtttc gtaagatact
     1441 actttgcctg agttttgagc cagaagattc aacgttctgt attggattgt tgtaaatttt
     1501 atttttttac cgttggcttt tatggcttgt tctgcgggaa acatttcaaa aggatagtca
     1561 tttttttcag aaaaagatcc ggagatttct gcaggattta aattttcaaa tgttttagat
     1621 tcttcatttt ttgtttttaa gtttcccaaa atttttctga tttttcttag tgctttttct
     1681 tttgcgtctg ttattctgcc ggtttcttca agccctaaat tttcagcatc ttttgtcaat
     1741 tcattttctt taaagaagga attaagagca aatctttgtt taggagttaa ttggtttatt
     1801 gcaaattcga ggtcattttt agaaatttcg gggttgaaaa tcttgaaaca atcttcttgt
     1861 tcattttcag ggagcgtact tgcaaatgtt tttgagcttc cttctcttac aggctcatcc
     1921 atataaagca agggggtggc aaacggaagc aacttggata acctttcttt tgacattttt
     1981 aatctttgcg caagttcgtt ttcgttaggc tgcctaaatt tttctgagta aaaattttca
     2041 atttcccgct taatcataga aatcttgtca ttatcatttt ttggtattga aattatattt
     2101 ttattttgtt ttaaacacga aagcatataa tatctaagct gacaatgcgc atatatcgta
     2161 aaatcacctt tttccggttt ataagcgtta atcaaatcaa ttaattttaa atttccggct
     2221 tgtacaagat ccaaaaaatc gttattttct ttggtaatag tttttgcaac acttacaaca
     2281 aggggcaaat aattttcaat tacctgattt cttgcattct cggctgtgtt tcctttgcct
     2341 tgggcttgaa aaattaatct atttatttct tctttggact ttctggaaag ttttgaaata
     2401 tctttaatgt atattgaaac agaatcggta ttaaaagaat ttttatcctc agcctttgtt
     2461 gattttccga aagaaatttt gtcttcacag tttttgtgag attttgattt aataaaatga
     2521 aaaccatttt ttgactttga agaagggaga attttatttt gatttgaaat aatattaaag
     2581 cttaacatat tatttttaaa atcggtaaaa aaaaatattg ctaaaaattg tttatacgga
     2641 aacaaaactt aacttaaacg ttaagcttat atccgccgcc gtaaattgtt tctatatatt
     2701 ttttgccgtt tgctattttg tctatttttg ttcttaaatg tcttatatga acacggattg
     2761 tttcaacatc atcatcgggc gcatagcccc aaatatcgct taacagtttt gccgaagata
     2821 ccattgttcc gtgattttgc atcaagaggt ggaaaatttc aaattcaatc ggggttaatt
     2881 ttgcctgctt ttcattgatt tttacagaat aaatttcggg caaaagcgta atttcattta
     2941 aggttaaaat ttcttttgtc gccgcagatt cagggatttg attagtccgt tttaaaagtg
     3001 ctcttattct gactcttaat tcttccattt caaaaggttt taccaaataa tcgtcaacac
     3061 ctatatcaaa gcctttaact ttgtcattaa gctgcgacag tgctgtcagc attaatatcg
     3121 gaatatgttt tgtgtcagga ttttgtctta ttctttgtgc gactgtgtat ccgtcaactt
     3181 cgggcatcat cacatcaagc acaattgcat ttggaagttc ctgctttgca agtgcaaagc
     3241 ctttgatccc gtctaaagcc tgaataacct tgtgccctgc aagttcaagg ttgattttta
     3301 caagctcatt tatgttttta tcgtcatcta ttactaaaat tttgttcatg taaatatttt
     3361 ataacaaaaa agcattataa gaaatgtaac aaaaattaat aaaatacatt gtttaaagga
     3421 gccattatta ctggattttt agtataaaaa atgtaaaaat ttattaaaat tttttaaatc
     3481 agttgacaca aatacaaaat tacggttttt tattgtaata tgtaaattga tatatttaaa
     3541 aaatcgcgta catagatacc aaaaggaggc acatgaattg gcagtaactt caccattttc
     3601 aagtattaat cagaacggaa aaacacaaat tcatttggga caacacgtat tagctgaatt
     3661 ttttgagtgt gaccccaata ttttaaacaa tgtagcttta gtagaaaaat tgatggtaga
     3721 tgcggctttg gaatgcggtg ctacaatagt tcaaaaatgt tttcatatgt ttaaccccta
     3781 cggcgtaagc ggagtcgtta taatagcaga aagtcatctg gcaattcata catggcctga
     3841 attaggttat gctgcggttg atttgttcac ctgcggtgac aaatgtgatc caaaagtgtc
     3901 ttataacttt ttaaaggaaa aatttaattc caaaaatgct tcttataaag agcttaaaag
     3961 aggaattatt gatgaagaaa ctatgaaagt tgctcattcg ccttttgaat tagctattta
     4021 ataaataaaa tattaaaaaa cgcgcaaaac cctaaagccc tgcgcgcccc actgattagg
     4081 gaatttattc ctgagcaatg ctgtcatgca tttctgcgcg gatattccca ctctcgttat
     4141 aagctgatat aaaatgcagg tatctttcca tcctgagttt taaccagcct aacattgtgt
     4201 ctgagccata gacttctata attcttgtcc ttgaaaaatg cctgtatgtg tagctgtaag
     4261 actcatctat gtaagttttg cacttacaat taagttcttc aactaaatca tacagcgttt
     4321 taagccatgc ggcctgaacg tttgtttcgt caactttgta ctcaagaatc atagacagct
     4381 ccttgtcttt tcaatggggg gtgtgttaaa gtttaattgc ggtcaaaatc ttttactgat
     4441 atatatggta tatacgttat atattataac ggatgttttg gttaaaaaaa agagtgtagg
     4501 ggtatgaaag tttacaattc ttcaaataat acggttatag cagaaagggc tgaagtggct
     4561 aaaagcttct tttcgcgcac tgttgggctt attttgaaaa aaactttgaa agagggcgaa
     4621 ggtttggtca taaaaccttg tttttcgata catacttttt ttatgaagtt tgcgatagat
     4681 gttttgtttg tcaataagaa aaatgaaatt gtcgcattat acgaaaatgt aaaaccgtgg
     4741 cgaattttgc ctgtacattt caattcttat tatgtagttg aacttccttc aggcacaatt
     4801 tcttcaacaa acatttctaa aggcgattcg ataaaattcg attaaatcat tatttacgtc
     4861 attctgaggg ggtaaatgta ttttgatttt tgtccgcccg ctcatatcac tgcgaggaag
     4921 aaatttattt ctgacgaagc aatctagaaa gcattaattt taattatttt ttgtaaacct
     4981 actttcttgt gtcgacaaga aagtaggcaa agaagctccc gaccggatct tcgttcgcta
     5041 atttacacta aactcaaccc aagctcgcga actcgctccg tttacacttc gctcaaacac
     5101 actcgctttg tcgttgtttc gttaagtgtc aattgctcac tccgataggg tctgataaga
     5161 gcccgtgcag ggtttcactc tgaaaccctt aattcgattg atgagtttgc tcatcaatcg
     5221 aaacgttctt taagcgaagg ctttgcctga gcaggcggcg cagccaaaag agtgtttttt
     5281 ctttctgcct tcttttgcct gaaacttgtt tcagtgcaaa atggcgtcac ggcttgcttg
     5341 aatagtccac tctttagcat ttattcacaa tagtgactag gttacttctt tcttttgccg
     5401 tcgatatata aaagaaagaa gtaacataat aaaacggttc atttcttgtc gacacacgaa
     5461 agtaggttta caaaaagaaa gaattattat cggtgggata gcaatcccac cctactatta
     5521 ttgaaaaatt gttatataat ctacttatga acaaaatttt gtccgaaaaa ttaaaactgc
     5581 tcccgtcttt acccggatgt tatttataca gcgatatcaa gggtgaaatc atttatgtcg
     5641 gaaaggcaaa aagcctaaaa aacagagtta aaagttattt taacaaaact cttgaaggtg
     5701 caaagcttaa tgtgcttgtg cctcagattg aaaacctcga atatattgtg accaattcag
     5761 aagcagaagc tttgattttg gaatcacact taataaaaaa gcacaaacca aagtacaata
     5821 ttttattaaa agacgataaa aaatatcctt attttttgat aacagacgaa gattttccgc
     5881 gaattatagt gacaagaaag cgcaatatga atcttgaaaa aggtaagtat tacggacctt
     5941 acacagatgc gcgcgccatg tatgcgactt tggatttttt gaaaaagatt ttcccgctca
     6001 aacagtgcaa acaacctaaa tttaaaagcc gcccatgtct ttactatcaa atagggcgat
     6061 gtcttgcgcc ttgtcaggga atggtaactt ccgaggaata taaaaaattg atagaacagg
     6121 cagagttgtt tttgcaagga aagcaaagcg agcttataaa atatttaaaa aatcaaattc
     6181 aggagtattc ggattctcag cagtacgaaa aagcagcaag acttcgcgat agttatatcg
     6241 atttgcagaa aacacttgag cggcaaaggg ttgtctttga aaatactaaa ttaaatcagg
     6301 atgttatctc attaatttat gatgacggaa tttttgccat tgttattttg atgattcgtg
     6361 aaggcagatt gattgataaa aaggattttg tttattttgt tgaattaccc ctcaccccta
     6421 ccccctctcc cgcaaggggc gaggggaatt cgcatggaat tgaaacattt accccctctc
     6481 ccgcaagggg cgaggggaat tcgcatggaa ttgaaacatt taccnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnccctct cccgcaaggg gcgaggggaa tggacaaaat
     6661 atggtcgtcg ggttaaaaga ttttatggaa acttttttca gagaatatta cggtaatctt
     6721 ttgttagaat ttcccgacac gattgtttca aatcagcttg aacttttagg tgaaaaaacg
     6781 ctctatcagg attggcttga acttttggcg aaaaagaaag ttaaaatcag ttacgcaaaa
     6841 agcacgcagg ggcaggagct tcaaaaactt gccgataaaa acgcaaaagt cttgttaggt
     6901 aatgctaaaa tcgaaaaaat gtctaaaata agagacgatt ttaatgaaat aggctcattt
     6961 ttagccgaaa aattaaagct taaaaacttt cctcatagaa ttgaatgtta tgatatttct
     7021 cacattcagg gcacaaaaac tgttgcttca atggtgactt ttataaacgg ggtaagcaaa
     7081 aaatcggctt acaaaaaatt taagattaaa cttgcagaag gcaaacccga cgattttctt
     7141 tcaatgaaag aggttttgac aaggcgatta aagcgtttag gcgatgaggg ttgggaaaaa
     7201 cccgatttga taataattga tgggggaaaa ggacagttgt ctgctgttat ggatattgta
     7261 aatgattttt ctgtttcgtc gttgcgagga gcttgcgacg aagcaatcca aaaaaataac
     7321 gctgataaga tcctgaaaca agttcaggat gacgtaacat taggaattga cattgtttca
     7381 atcgcaaaac gcgaggaaga aatttttctg ccaaatcaat caaaacccgt aattttgccg
     7441 cgtaattctt cagctttatt tttgttccaa agaatccgtg atgaggctca ccgctttgca
     7501 attacttatc acaagaagct tcgcgagcgt gcggcggtga aaaaataatc tttttgtgct
     7561 aaaatagtca tgatgtgagg gcgaggtagc tttaaatgga atttattcag aattttgttc
     7621 atgcaaacag tattttggtt tcggttgcca ttttgctttt agcttatttt tttattgcaa
     7681 cggaaaaagt gcctaaagtt actattgctc ttgcgggtgc aaccattgca atacttttag
     7741 ggcctttatc tcaggaaaaa gggatgcagg gagaattaaa tcctgcgtat tttataaatt
     7801 acattgattt taatgttata tttttgcttg tttcaatgat gcttatcgta aatatcgccg
     7861 caagaagcgg tatgtttaac tggtttgcca cagaactttt gagacttacc aaaggacatc
     7921 cgaaatctgt tttatttgtt ttaggtgttt ttaccgcaat agcctcggct tttttggata
     7981 acgttactac cgtaattctt gtaatgcctg ttacttttgt tgtcgcaaga gaattggata
     8041 ttactcccgt tcctttcctg ataacggaaa ttttgacttc aaatataggc ggaactgcca
     8101 ctttaatcgg tgatccgccg aatataatta tcggaagtgc ggcagggctt tcatttttgg
     8161 attttttgct tgaattaacc gatattgttt tgattatttt gcttataagt ttagcgatat
     8221 tggtttttat gttcagaaaa gttttaaaat catctcctga aaaaatggaa catattgcaa
     8281 agcttgataa ttcaggcact ataaaagatt acaatcttat gattcgttct tcgatagtcc
     8341 tttttcttgt tgttttgggt tttgttttgc acgatgttat acatgttcaa tcctgtattg
     8401 tcgcaatggc gggggcttct tttcttttga tgtttgaaaa gccgcatcaa attttgcatg
     8461 atgttgaatg gaatacgatt ttctttttta tcggactttt tattataatc ggaggtgttg
     8521 aagcctctgg tggaataagt cttatggcac agtggctttt ggatattaca aacggatcgc
     8581 aaaaagtcgc cgctatggtt attctgtggg cttcgggaat tttatccgga attattgaca
     8641 atatccctta tacagtaact atggcaccta tgcttttgga agtccaaaag gctatgggtg
     8701 aagcttatat tcatccgctt tggtggtgtc tgtctttggg tgcg
//
LOCUS       DAOQAU010000063         8742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_9559_length_8742_cov_6.942558, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000063 DAOQAU010000000
VERSION     DAOQAU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9559_length_8742_cov_6.942558"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKI94_07835"
     CDS             <1..729
                     /locus_tag="PKI94_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26681.1"
                     /translation="IAMSYTQAGKQDKAIEAYSKVLALKPNPTLYEYATTGKMCLETP
                     DKCKIGDDSGELEKMIRYNYGKGLSNAVRKDLEQKRLDVIKQEMNSDKEMDSYDLRKF
                     KDYSNQRSKIEQPEKLSMTVEETNATLLQKSEQPKQPTNDEIVAALKVLNQAGLTNFA
                     NPMAQVNNPMLNAQSAEMAQMSMMLGGNNMQNNNNNNAMFNMLPFMMAQKPNGESSYT
                     PQLMQAMIMNSMMPDFNYNLNENK"
     gene            744..1184
                     /locus_tag="PKI94_07840"
     CDS             744..1184
                     /locus_tag="PKI94_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNW26682.1"
                     /translation="MKIKTTRFGEIEVSEKFIFDFSEPIIGYEKEKQFALIEHKENSA
                     FRWLQAVDFPEVAFVVTVPGFFGIDYSFELPETTQETLGIKEAEDILSLNIVVIPREN
                     PRKSTVNLLAPIIINIKNNKAGQIVLNSNDFPVDFPLFEKEAVC"
     gene            1178..1456
                     /gene="csrA"
                     /locus_tag="PKI94_07845"
     CDS             1178..1456
                     /gene="csrA"
                     /locus_tag="PKI94_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNW26683.1"
                     /translation="MLVLSRKNGQKIIINDDIEITILESRFDHCKIAIEAPKSVKIYR
                     EEVYKQIQFANTMSNKTSLESLDSLTSLMQAQNDIMPEKTNKIFIKKK"
     gene            1459..2055
                     /locus_tag="PKI94_07850"
     CDS             1459..2055
                     /locus_tag="PKI94_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26684.1"
                     /translation="MVSELLYQVAKEKFLSGDISSVQKFFEENSYFLELAYCKMLSGK
                     LKEAKEIFSKNSEDDLRADWGKRFIQFIEGHVTTAPTYFQIRNFLEIDLNLLIKAGRA
                     DYVENIINGADIFYSVNPESYKFIARVMYNNDFIDLALYFLGKAKENFYYDPEMHLIF
                     ANCYLKKGQKELAKQSVNNCLSILPEYAPAKKLSLQLM"
     gene            2084..5044
                     /gene="uvrA"
                     /locus_tag="PKI94_07855"
     CDS             2084..5044
                     /gene="uvrA"
                     /locus_tag="PKI94_07855"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNW26685.1"
                     /translation="MNEKIIITGAKEHNLKNVSLELPKNELIVFTGVSGSGKSSLAFD
                     TIFAEGQRRYVESLSTYARQFLGQMDKPNVEHIEGLSPSISIDQKSTSNNPRSTVGTV
                     TEIYDYLRLLYARIGTPYCPECGDEIKPQTIDEIVDKILGLGEGAKIQILAPIARGKK
                     GEFQSTFEELRQEGFVRVKVDGQIFNLDEDEIKLEKNIKHTIHVVVDRIVVKDEAISR
                     IADSVQIALKKADGIIIVDVVEYTPHPSPPPQGGREQNLTNPSPLAGEGEFHGEATSH
                     RNLGEGSKEIIFSEKLSCPKCNLSFEELAPRIFSFNNPYGACERCSGLGADNVVNPNL
                     VIPDRKKSLSQGAIYPWAKTTNQYYADLINSVCEYYGIDKDKSFEDLTQDEQNIILYG
                     GDDELIKIRVKDFVTKRYGTHYMKFPGVINYLEKKYKENIDSEYWSSEIQKYMITTPC
                     TACGGARLKPFPLAVKINDKNIYEFTLLPIDEAFNFISDLFLELSDYQMKIAKQVLEE
                     IRDRLKFLLDVGLNYLTLARMAGTLSGGEAQRIRLATQIGSGLSGVLYVLDEPSIGLH
                     QRDNDRLIQTLIKLRNLGNTLIVVEHDEETIKHADYIVDIGPNAGINGGEIVAKGSID
                     DIVNCKKSITGKYLSGEKTIPVPQKKREGSGHFLRVKNARLNNLKNIDVDVPLGRISV
                     LTGVSGSGKSTLMQDLIYEYAKYKLLKNKPKPQGIDEIEGFEHIDKVIDIDQSPIGRT
                     PRSNPATYTDVFTHIRDLYSRTNEARVRGYKPGRFSFNVKGGRCEACKGDGVLKIEMN
                     FLSDVYVKCDVCKGKRYNRETLEVKYKGKTISDVLEMSVGEALEFFENIPSIKNKLKT
                     LNDVGLDYIKLGQSATTLSGGEAQRIKLASELNKRATGKTLYLLDEPSVGLHWYDLDK
                     LIKILQKLADSGNTILIIEHNMDLIKIADYIIDLGPEGGNAGGEIVATGSPKEVSEME
                     KSHTGKYLKKVFNS"
     gene            complement(5041..6162)
                     /gene="ald"
                     /locus_tag="PKI94_07860"
     CDS             complement(5041..6162)
                     /gene="ald"
                     /locus_tag="PKI94_07860"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNW26686.1"
                     /translation="MIIGVPKEIKSWENRVSLTPSGAKTLIENGHKVFVQTTAGLGSG
                     FTDEEYEKIGAVILPTIEEVYQKSEMIIKVKEPQPSEYGLMKEGQLIFTYLHLAVEPE
                     LTKALLKRKVTGIAYETIQKECGELPLLTPMSEIAGRMAVQIGASFLENPHGGRGVLL
                     GGVPGVPPAEVVIVGAGVVGLNATKIAYGMGANVTVIDLNLHKLRYFDDIFHGKVTTL
                     MSNSLNIEECAKRADLMIGAVLIPGAKAPHLVSEETVKKMKKGAVIVDVAIDQGGIFE
                     TIDKVTTHENPVYEKHGVMHYSVANIPGAVARTSTIALTNATLPYALNLANKGFIKAA
                     KSCSELAKGVNTCQGHLVSEPVAESLGLEHTELSQIIGF"
     gene            complement(6162..6929)
                     /gene="pyrF"
                     /locus_tag="PKI94_07865"
     CDS             complement(6162..6929)
                     /gene="pyrF"
                     /locus_tag="PKI94_07865"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNW26687.1"
                     /translation="MKITKPASAKDRIVLALDVDNINTAKDIVTELKDYVGFFKVGLQ
                     LLTSCGFEAIKMVKETGGKVYCDLKFHDIPNTVAHACANLVKRNIDFFNIHLQGGSKM
                     VSQVVKAAKETSKDLNTEPPTILGVSLLSSFGQRTLTQELCVDKNIEEYIMQLALMAK
                     ETGLDGIVASAAEARKIRNELGDDFIIMCSATRPTWAAVNDQVRVDTPTEAINAGVDY
                     LVIGRPVTEAPDRIAAVNLIIDEINTALNEQVILGGR"
     gene            7054..7398
                     /locus_tag="PKI94_07870"
     CDS             7054..7398
                     /locus_tag="PKI94_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26688.1"
                     /translation="MSEFHNLEESLKEFLSAQQANTPNAQNINLYKYNNLKIFMMSKQ
                     NKNPHFIVRMGISETVYSLNNGEKLSGGLGADERWVRKWFENPINKASLQELWKRSLK
                     VETVQVDTSEEE"
     gene            complement(7462..7845)
                     /locus_tag="PKI94_07875"
     CDS             complement(7462..7845)
                     /locus_tag="PKI94_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26689.1"
                     /translation="MKKTLLALGILMTLIISSQTTFAACPCSNPCPCSEPCASACPAC
                     PCDCACPACPCDCACPACPCDPCCDKCCKKKCTWWKIFENKCCCKRCDDCGCTCKRCH
                     WWKFWEDRRCVNNGSNCNKCNKCCD"
     gene            complement(8074..8394)
                     /locus_tag="PKI94_07880"
     CDS             complement(8074..8394)
                     /locus_tag="PKI94_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26690.1"
                     /translation="MKKMLVSLFAIVALAIGSQTTFAACPCSNPCPACPVIAPCCSTA
                     MPCPACPVLDPCSCGHQSCCNSCCDSCNSCCDNCCKKKCRWWKFWENKNCCYKCNSCC
                     DCCD"
BASE COUNT         2882 a         1521 c         1722 g         2617 t
ORIGIN      
        1 attgcaatgt cttatactca agccggaaag caagataagg caatagaagc ttattcaaaa
       61 gtattggcac taaaacctaa tcctacattg tacgaatatg ccactacggg taaaatgtgt
      121 ttggaaactc ctgataaatg taaaattggg gatgattccg gcgaattgga aaaaatgatt
      181 agatacaatt atggaaaggg tctttcaaat gcagtcagaa aagacttgga gcaaaaaagg
      241 cttgatgtaa ttaagcagga aatgaatagt gataaagaaa tggatagtta tgatcttaga
      301 aaatttaaag actattcaaa tcaacgctct aaaatagagc agccggaaaa actttctatg
      361 actgttgagg aaaccaatgc aacacttctt caaaaaagcg agcagccgaa acaacctaca
      421 aatgatgaaa ttgtagctgc gcttaaggtt ttaaatcaag cagggcttac aaattttgca
      481 aatccaatgg ctcaagttaa taatcctatg ttgaatgctc aaagtgctga aatggcacag
      541 atgagcatga tgcttggcgg taataatatg caaaacaaca acaataataa tgcgatgttt
      601 aatatgctac cttttatgat ggcgcaaaaa cccaacggcg aaagcagtta tacacctcag
      661 cttatgcagg ctatgattat gaattcaatg atgcctgatt ttaactacaa tcttaatgaa
      721 aacaaataaa aagagagaaa attatgaaaa taaaaacaac aagatttggc gaaatagaag
      781 tttcagaaaa attcattttt gatttttccg agccaattat aggatatgaa aaagagaaac
      841 aatttgcact tatcgaacac aaagaaaatt cggctttcag gtggcttcaa gctgtcgatt
      901 tccccgaagt tgcttttgtg gttacagttc cgggtttttt cgggatagat tattcttttg
      961 agcttcccga aacgacacaa gaaacacttg gaataaaaga agccgaagat attttgtcgc
     1021 ttaatattgt tgtaatcccg cgcgaaaacc cgcgcaagtc aacggtaaac cttcttgcgc
     1081 ctattattat taacataaaa aataataagg caggacaaat tgttcttaat agcaatgatt
     1141 ttcccgtaga ttttccttta tttgaaaaag aggcagtatg ctagttttat ccagaaaaaa
     1201 cggacaaaaa attataataa atgatgacat cgagattaca attttggaat ctcggtttga
     1261 tcattgcaaa attgctatag aagcacccaa aagtgttaaa atatacaggg aagaagttta
     1321 taaacaaatt cagtttgcaa atacaatgag caataaaaca tcgcttgaaa gccttgattc
     1381 tttaacttct ttaatgcagg cacaaaatga cataatgcct gaaaaaacca ataaaatatt
     1441 tataaaaaag aaataattat ggtgtcagaa ttactttatc aagtcgcaaa ggaaaaattt
     1501 ttgtcgggtg atatttcatc tgttcaaaag ttttttgaag aaaattctta ttttttggag
     1561 ttggcttatt gcaaaatgct ttcagggaaa ttaaaagaag caaaagaaat tttctccaaa
     1621 aatagtgagg acgatttgag ggcagattgg gggaaaagat ttattcaatt tattgaaggg
     1681 catgtaacta ctgctcctac atattttcag ataagaaatt ttcttgaaat cgatttgaat
     1741 ttacttatta aagcaggcag agccgattat gtcgaaaata ttataaacgg tgccgatatt
     1801 ttctactctg taaaccctga aagttataaa tttattgcca gagtaatgta taataatgat
     1861 tttattgatt tggctttata ttttttgggc aaagcaaagg aaaattttta ttacgaccct
     1921 gaaatgcatc ttatttttgc aaattgttat ctaaaaaagg ggcaaaaaga actcgcaaag
     1981 cagtctgtaa ataactgcct gtcgatttta ccggaatacg caccggctaa aaaactctcc
     2041 ttacaattaa tgtaatattt tttttatatg cgataattta tttatgaatg aaaaaattat
     2101 tataacaggg gctaaggagc ataatttaaa aaacgtttct cttgagctgc cgaaaaatga
     2161 attaattgta tttacgggag tttcaggctc gggtaaaagt tcgcttgctt ttgatacgat
     2221 ttttgcagaa ggacaaaggc gatatgttga aagtctttca acatacgcac gccagttttt
     2281 gggacaaatg gacaaaccta atgtcgaaca cattgaaggt ctttctccgt ctatttccat
     2341 agaccagaag tcaaccagta acaatccgcg ttcgactgtc ggaacggtta ccgaaattta
     2401 tgactatctc agacttttgt atgcaagaat cggtactcct tactgcccgg agtgtggaga
     2461 tgaaattaag cctcaaacaa tagatgaaat cgttgataaa attttgggat taggtgaagg
     2521 agccaaaatc caaattttgg cacctatagc ccgcgggaaa aaaggcgaat tccagtcaac
     2581 gtttgaagaa ttaagacagg aaggctttgt ccgcgttaaa gttgatggtc agatttttaa
     2641 ccttgatgaa gacgaaatca aacttgaaaa aaatattaaa catacgattc atgttgtggt
     2701 ggacagaatt gtggttaaag acgaggcaat atcaagaata gcagacagcg ttcaaatagc
     2761 cctcaaaaaa gccgacggga taattattgt ggatgttgtc gaatataccc cccaccccag
     2821 ccctcccccg caagggggga gggagcaaaa tttaaccaat ccctcgcccc ttgcgggaga
     2881 gggagaattt catggcgagg ctacgagcca tagaaattta ggtgaggggt caaaagaaat
     2941 tatcttcagc gaaaaacttt cctgtccgaa atgtaactta agttttgaag aacttgcacc
     3001 caggattttt agttttaaca atccttatgg tgcgtgcgaa agatgctcgg ggcttggtgc
     3061 cgacaatgta gttaacccta atctggttat tcccgataga aaaaaatcac tctcgcaagg
     3121 agcgatttac ccttgggcaa aaacaacaaa ccaatattat gccgatttga taaattccgt
     3181 ttgcgagtat tacggcattg ataaagacaa atcctttgaa gatttaaccc aagatgagca
     3241 aaatataatc ttatacggcg gtgatgacga gcttattaaa ataagggtga aagattttgt
     3301 gacaaaaaga tatggcacac attatatgaa attccccggt gttatcaatt atctggagaa
     3361 aaaatataag gaaaatatag attccgaata ttggtcaagc gaaatccaaa agtatatgat
     3421 tacaacacct tgcaccgctt gcggaggagc aaggcttaaa ccgtttccgc ttgcggtaaa
     3481 aattaatgat aaaaacattt atgaattcac tttattgccc atagatgaag catttaactt
     3541 catttcagat ttgtttttgg aattgagtga ctatcaaatg aaaattgcaa agcaggtttt
     3601 agaagaaatt cgtgacagac ttaaattttt gcttgatgtc gggcttaatt atcttactct
     3661 tgcaagaatg gcaggcactt tatcaggtgg tgaggctcag agaataaggc tcgcgaccca
     3721 aataggaagc ggactttccg gagtgcttta tgtgcttgac gagccttcaa tcgggcttca
     3781 tcagcgggac aatgacaggt taattcagac tttgataaaa ttgagaaatc tcggaaacac
     3841 tttgattgtg gtcgagcacg atgaagaaac aattaaacac gcagattaca ttgtggatat
     3901 aggacctaat gcgggaatta acggcggtga aattgtcgca aaaggcagta ttgatgatat
     3961 tgtgaactgt aaaaaatcaa ttacgggtaa atatttaagc ggcgaaaaaa caattcctgt
     4021 tccgcaaaaa aaacgagaag gaagcgggca ttttttacgg gtaaaaaatg cccgacttaa
     4081 caacttaaaa aatatcgatg ttgatgttcc gctgggtaga atttctgtac tcacaggggt
     4141 ttcaggctcg ggaaaatcaa cactgatgca ggatttaatt tatgaatatg caaagtataa
     4201 attgcttaaa aataagccaa agcctcaagg aattgacgag attgaaggat ttgaacatat
     4261 tgataaggtt atcgatattg atcaatcgcc tataggaaga actcctcgct caaatcctgc
     4321 aacttatacg gatgttttta cccatattcg tgatttatat tcaaggacaa acgaggcaag
     4381 ggtcaggggg tacaagcccg gaagatttag ctttaatgtt aagggcggac gctgcgaagc
     4441 ttgtaagggt gatggcgttt tgaaaatcga aatgaatttt ttgtctgatg tttatgttaa
     4501 atgcgatgtt tgtaaaggga aacgctataa cagagaaact ttggaagtca aatacaaagg
     4561 aaaaacaatt tctgatgtgc ttgaaatgag tgtgggcgag gctttggaat tttttgaaaa
     4621 tattccttcg attaaaaata agttaaaaac tttaaatgat gtagggcttg attatattaa
     4681 actcgggcag agcgcaacaa cgctttcagg cggtgaagca cagaggatta aactcgcatc
     4741 cgaattaaat aaaagggcaa cgggaaaaac cttgtatttg ttggatgagc cttcggttgg
     4801 tctgcactgg tatgatttgg ataaattaat taaaatattg caaaagcttg cagacagcgg
     4861 aaatacaatt ttgataattg agcacaatat ggatttaatt aaaattgcag attacataat
     4921 tgacttaggc cctgaaggcg gaaatgccgg cggagaaatt gttgcaacag gcagtccaaa
     4981 agaggtgagc gaaatggaaa aatctcacac aggaaaatat ttaaaaaagg tttttaactc
     5041 ttaaaagcca attatttgtg ataattcggt gtgttctaag cctaaagatt ctgcaacagg
     5101 ctctgaaaca aggtgcccct ggcaggtatt tacacctttt gcaagttcag aacaggattt
     5161 tgccgctttg atgaagcctt tgttcgcaag attgagtgca taaggaagtg ttgcgtttgt
     5221 aagtgcaatg gtcgaagttc ttgcaacggc tcccggaata tttgcaacag agtagtgcat
     5281 tacaccgtgt ttttcataaa cgggattttc gtgagttgta actttgtcta ttgtttcgaa
     5341 aattccgccc tgatctatcg caacatcaac tataacagca ccttttttca tctttttaac
     5401 ggtttcttcg ctgactaaat gcggggcttt tgcgcccgga atcaggaccg cacctatcat
     5461 caaatcagct cttttggcac attcttcgat atttaaactg tttgacatca aagttgttac
     5521 tttgccgtgg aaaatatcgt caaaatatct tagtttatgg aggtttaaat caattactgt
     5581 aacgtttgcg cccattccgt aagctatttt tgttgcattt aagccgacaa cacctgctcc
     5641 gacaattacg acttcggcag gcggaacacc tggaactcca cctagcaaaa cgcctctgcc
     5701 gccatgcgga ttttcaagga atgatgcacc tatttggact gccattcttc ctgcaatttc
     5761 gctcattgga gttaaaagag gaagctcgcc gcattctttt tgaattgttt cataggcaat
     5821 tcccgttact ttgcgtttta aaagagcttt ggttaattca ggctctacgg caaggtgcag
     5881 ataggtaaat ataagttgcc cttctttcat cagtccgtat tcgcttggct gcggctcttt
     5941 tactttaatt atcatttcag acttttgata gacttcttct atggtcggaa gtataacagc
     6001 accgattttt tcatattctt catctgtaaa accgctgcct aatccggcgg ttgtttgcac
     6061 aaagactttg tgaccgtttt caattaaggt ttttgctcct gagggggtca gagaaactct
     6121 gttttcccaa ctttttattt cttttggaac tcctataatc attatcttcc tcctaaaata
     6181 acctgttcgt tcagtgcagt gttgatttca tctataatta aatttacggc cgcgattcta
     6241 tcaggggctt ctgtaacagg acgtcctata acaagataat ccactcctgc attaattgct
     6301 tcagtcggag tatcaaccct tacctggtcg tttaccgccg cccatgtggg tcttgttgca
     6361 gagcacatga ttataaaatc atctcccaat tcgtttctta tttttctagc ttctgcggca
     6421 cttgccacaa ttccgtcaag gccagtttct tttgccatta aagcaagctg cataatgtat
     6481 tcttctatgt ttttgtcaac acataattcc tgtgttaggg ttctttgacc aaaacttgat
     6541 aataaactta cacctaaaat agtcggaggc tctgtattaa gatcttttga agtttctttt
     6601 gccgctttta caacttgtga gaccattttt gaaccgcctt gtaaatgtat attgaaaaaa
     6661 tcaatattac gtttcactaa attggcacag gcatgcgcaa ctgtatttgg aatatcatga
     6721 aattttaagt cgcaataaac tttgccccct gtttctttaa ccatttttat ggcttcaaaa
     6781 ccgcagcttg taagtaactg taatccaacc ttaaaaaagc ctacgtaatc ttttaattcg
     6841 gttacaatat cctttgcggt attaatattg tcaacatcca aagctaaaac aatacgatct
     6901 ttggcacttg caggttttgt gattttcatt tttcccccaa aataacacaa ttaataataa
     6961 atactaccac ttagccaatc taaaatcaag aggggattgc agattgattt tttaagtaaa
     7021 atactttaaa attgtttttg taagaggtat tttatgtcag aatttcataa tcttgaagaa
     7081 tctttaaaag aatttttatc tgcgcaacag gcaaatactc ctaatgccca aaatataaac
     7141 ctgtataaat ataataattt aaagattttt atgatgtcaa aacaaaacaa aaaccctcat
     7201 tttattgtaa gaatgggaat atctgaaacg gtttacagtt taaataacgg ggaaaaattg
     7261 tcgggcggat tgggtgctga tgaaagatgg gttagaaagt ggtttgaaaa ccctataaat
     7321 aaagcctctc ttcaggaatt atggaaaagg tcgcttaagg tcgaaactgt tcaagttgat
     7381 acttcagaag aagaataatt taaaaacagg gcgcaaaccg aaggtttgcg cccgaaaatt
     7441 ataaagtcac tttttaggtt tttaatcaca gcatttattg catttattgc aattgctgcc
     7501 attgtttaca caacgtctat cttcccaaaa tttccaccag tggcatcttt tacaagtaca
     7561 gccgcagtca tcgcatcttt tacaacagca tttgttttca aaaattttcc accaggtaca
     7621 ttttttcttg cagcatttgt cacagcaagg atcgcaaggg caagcaggac atgcacagtc
     7681 acaaggacaa gcagggcatg cacagtcaca agggcaagca gggcaagccg aagcgcatgg
     7741 ttctgaacaa ggacaaggat ttgaacaagg acatgccgca aaagttgttt gagaactaat
     7801 gattagtgtc attaaaatcc ccaatgccag taatgttttt ttcataaaaa tctcctttta
     7861 actacttttt taagttcctc acttaaattt atacgtaact tgttattgat ttaattgtat
     7921 aaccgcaata ctaaaagacc atatctgaac aggtaatatt taagagtaat tgtgaattaa
     7981 acacaaaggc aatgaataaa aagcggaccg aaaactaaag gttttcggtc cgcttccatg
     8041 agattaaaaa actttaacta taagtttcag gttttaatcg cagcagtcac aacagctgtt
     8101 gcatttatag cagcagtttt tgttttccca gaatttccac caacggcatt tctttttgca
     8161 acaattgtca caacaggaat tacaactgtc acaacaagaa ttgcaacagc tttggtgtcc
     8221 gcagctgcaa gggtctaaaa cagggcatgc aggacaaggc attgctgttg agcagcatgg
     8281 tgctataaca gggcatgctg ggcaggggtt tgaacacgga caagccgcaa aggttgtttg
     8341 cgaacctatt gcaagtgcaa cgatagcaaa cagggataca agcatttttt tcatgattaa
     8401 atctcctttt ttgatttttt gtccctgata aagagggact ttttctaaaa aaatacactc
     8461 taattgtatc ctcataaata aaaaaaacga tgtctgttag tacaatattt aggtaaggta
     8521 attattacct gatggggtaa tggaaaaaat aacttttgtg cttttatgaa gttaaggcag
     8581 ggttttaaag tagtctttta cgtacagagc gttatttgca caaccgaatc cgctgctgtt
     8641 tgcattgctt gatgttgtac aatattccgg ataactgctt tttgcagagc ctatcggaat
     8701 taaagaatca tttgacaaga tttgaaaagc aaacatatcg ta
//
LOCUS       DAOQAU010000064         8093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_10642_length_8093_cov_6.124409, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000064 DAOQAU010000000
VERSION     DAOQAU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10642_length_8093_cov_6.124409"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /gene="trxB"
                     /locus_tag="PKI94_07885"
     CDS             <1..953
                     /gene="trxB"
                     /locus_tag="PKI94_07885"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNW26691.1"
                     /translation="RGKKMKTYEFDTVILGGGPAGLSAAIYAARGASSTAIIDISMLG
                     GQPTNYLELENYPGFGLIDGFDLMENFEKHVDRFDVKKFPMQEIDKIDLTAQIKIIQT
                     KEATFKAKTVILATGAKSKKLGISGEKEFLGRGVSYCAVCDGAFYKDKNVAVIGGGNA
                     AVEEGMYLTKFAKKVYIIHRRDTLRADKIIQERAFKNKKVEFVFDAIPVEILGDDLVN
                     TLVLKNVKTNETTNLEVNGVFPYIGFSPNVEGFDGQIKQDGNGFIITDERMQTSIEGV
                     FAVGDIRKTPLRQVITAASDGAVGAVFATKYLETLSEIMV"
     gene            complement(950..1750)
                     /locus_tag="PKI94_07890"
     CDS             complement(950..1750)
                     /locus_tag="PKI94_07890"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNW26692.1"
                     /translation="MQKTLDFINKKTNNFQPEIGIILGSGLGDFASEFESINIPYSEI
                     PDFETSTVQGHKGQLVFAQICGKRVVIMQGRYHFYEGHSMQKITYPVKVMKKLGVKTL
                     IITNAAGAVNKAFNPADLMVITDHINLMGTNPLVGSNDETLGPRFPDMSEIYKKNLIK
                     IAEESAKELKIKLRKGVYAAGAGPSYETPAEVKMLRIIGADVTGMSTVPEAIVANYCS
                     MDVLGISCISNYAAGITDLKLSHAEVIETTNVVKNKFKNLILKILERI"
     gene            complement(1919..2158)
                     /locus_tag="PKI94_07895"
     CDS             complement(1919..2158)
                     /locus_tag="PKI94_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26693.1"
                     /translation="MEQIIKVAWDLNENTENRYQLVYEIAELAKKLVDETQAKKSREE
                     FGFDEYYESSYKKENPVEHAIMVKASEADDNRLVG"
     gene            2264..2905
                     /locus_tag="PKI94_07900"
     CDS             2264..2905
                     /locus_tag="PKI94_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNW26694.1"
                     /translation="MKKRLVWIAELFIWLLILFVIAGSIMFARYNYRKHFNTYQIFLP
                     DVDGLIVGSPVRLMGIQVGYINQINIVGDDVYVKFVITEKNMKVPQGSTATVEFTGLG
                     GSKSLELYAPTTENKFLSDKFIIPMPPKRIHDSLGLLNDMFDKIIGITYTISNFMDQI
                     GIMKYNNGKLQNNLTVTQILNLTSKWIEEATEKCENCKIKDKENGFTKNKSNK"
     gene            2874..3524
                     /gene="upp"
                     /locus_tag="PKI94_07905"
     CDS             2874..3524
                     /gene="upp"
                     /locus_tag="PKI94_07905"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNW26695.1"
                     /translation="MDLQKTRVINNPVILQNLCTMRDKNTDCQGVRIATRKITRVLLY
                     EAAKGLPLIEKTITTPLTVFKAQTIDPDINIIISPILRAGLIFTDEAIDILPQACIRH
                     IGMYRDEKTLKPVWYYNKVPMKVKDPHKFYIFITDPMLATGNSLLEAIKLYADKKIPI
                     ENIKIISIIAAPEGLKNIHSKYPEVEIITAAIDSHLNEHGYIVPGMGDAGDRIFNT"
     gene            complement(3521..5128)
                     /locus_tag="PKI94_07910"
     CDS             complement(3521..5128)
                     /locus_tag="PKI94_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26696.1"
                     /translation="MYNFQKTNNECSECLSRGICSDSPILSSLQEIILLYLKELSFYL
                     IKLKEFGVKNEQIKETVIYSLGNLALSVEYNQQQFRELTNKLNDYVVESKTIYKNFCE
                     KNSLDIESIKTYFKHHKNIYLSDAIKRGEKYFLKKAELLNPNQKALFDIMVFLIKSMS
                     IKLMELKRMGKDHDQGYYAILSLLSSINVTNFSQEQAKRAIDDVLDIYYDILRTIFYT
                     QNELYGPIRQTEVASSAQLGKAILVSGSDFNKLERVLKACEGKEIGVYTHGVEMLFAH
                     AFPKLQSHPNLKGHFGSGFDSYMMDFASFPGSILMTRINLEKSEYLYRGRLFTMDPVA
                     PPGIIKILDGDFEPLIKAALESRGFNKIIEKPPIKVGFSYDEIEYKMAEITDKILNKQ
                     IKHLYIVGLLNYPVLQKQYFESFFELLPSDCYAISLSYNKNRENVYHLDRINDYVLFY
                     RILKEIKNKIPLNKMPLSIFLTRCDKHTISNLLYLSHLGIKHIYMGKCATNIINPTLF
                     NTIKEVYGIKEISDPKTDLEETLGQQL"
     gene            5260..6225
                     /locus_tag="PKI94_07915"
     CDS             5260..6225
                     /locus_tag="PKI94_07915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26697.1"
                     /translation="MKKVFIISPKSIAGELIIDGFACAFKNIGYRVLLKKIDEVTLNE
                     LEKFNPDIIFGYDYSYLTDQGCKEIIQKIQFKNRIFYFGDEPKSKFSLGEKNELYDEL
                     SNLDATVFVWDKAFTKEFKKCYYLPLAVNPLKYATEFSGYKYAITFVGRPLTDKRQKI
                     LSCLVKSFGNKLNIFCYEKHFEKSVEEIKEKKLLEGHELEIYKKSYKGFVKTESELAK
                     IYNSSEINLNITEQGKSSLNYRVFEVLASGGFLLTDEKKDVQKYFIPSKQLETYKNET
                     DLTDKIEFYFQNPGIAQRIAHLGRYECIKSHSFDERIKTIIKKIT"
     gene            complement(6222..7595)
                     /gene="radA"
                     /locus_tag="PKI94_07920"
     CDS             complement(6222..7595)
                     /gene="radA"
                     /locus_tag="PKI94_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNW26698.1"
                     /translation="MAKVKTKWICQECGYESAGYLGKCPECSAWGSLVEEVQQNFSPV
                     VLNSEFFDNKPSLINEIEINEEIRTQSSIKEFDRVLGGGFVTGSVTLLAGDPGIGKST
                     LVLQACGLLCRQGKKVLYVCAEESANQIKLRAQRLGVNEIGLYIYPQTNFELIKKEIE
                     NIKPDFVVVDSIQAIYSQEVSSSAGSVSQIRECCNFLIQIAKNQNITMIVIGHVTKDG
                     NIAGPKVLEHMVDTVVYFEGDKYKSYRLLRSVKNRFGNTAEAGIFEMGINGLAEVINP
                     GGIFIQEHSQEVTPGSVIISTSEGTRPLLVEIQALVGTTPYPSPRRVANGVDFGRVLQ
                     ILAVLEKRVGLNLSKQDVYVNVIGGIEISEPAADLGIALAVATCARDVVVDSHTVIIG
                     EIGLSGEIRAVQGIDKRIKEAQILGFKKAIIPFANKDFEQIKGIEIIKAKRLIDAISA
                     CVSRVEA"
     gene            complement(7618..>8093)
                     /locus_tag="PKI94_07925"
     CDS             complement(7618..>8093)
                     /locus_tag="PKI94_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26699.1"
                     /translation="AIFELALYWQDVNAVYSLNNEINSNVALVDLKDFNMGLPCPAVS
                     VAQSILEKKDSAITLNNPGYSVSSPQTGTLADVEPFALYKIVSANQVNGNPQVSVWVD
                     CRSPFENGIVTQVEFYHKTLIMKASIPRFDKPEPIVIIPDNIFISSPKLVTSRHY"
BASE COUNT         2667 a         1475 c         1373 g         2578 t
ORIGIN      
        1 tgagaggaaa aaagatgaaa acttatgaat ttgatacggt aatattagga ggcggacctg
       61 cggggctttc tgcggcgatt tatgctgcaa gaggggcatc ttctacagcc ataatcgata
      121 taagcatgtt gggcggacag cctacaaatt atcttgagct tgaaaattat ccgggcttcg
      181 ggcttataga cggctttgat ttaatggaaa attttgaaaa acacgtcgac agatttgatg
      241 tcaaaaaatt tcccatgcaa gaaatagata aaattgacct tacggctcaa attaaaataa
      301 tccagaccaa agaagctact tttaaagcaa aaaccgtaat tcttgcaaca ggtgcaaaat
      361 ccaagaaact gggaatcagc ggagaaaaag aattcttggg aagaggggta agctactgtg
      421 cggtgtgcga cggggcattt tataaagaca aaaatgttgc tgtaataggc ggcggtaacg
      481 cggcagtcga agaagggatg tatttgacaa aatttgccaa aaaagtctat atcatacaca
      541 ggcgcgatac tctaagagca gacaaaatca ttcaggaaag agccttcaag aacaaaaaag
      601 tggaatttgt ttttgacgcc attcctgttg aaattttagg tgatgacctt gttaacaccc
      661 ttgttttgaa aaacgttaag acaaacgaaa ccacaaatct tgaagtaaac ggcgtttttc
      721 cttatatagg cttcagcccg aatgtagaag ggtttgacgg tcaaattaaa caagacggaa
      781 acggatttat tataacagat gaaagaatgc aaacttctat tgaaggagtt tttgcagtcg
      841 gcgacataag aaaaactccg ctcagacagg ttataacagc agcttcagac ggggctgtcg
      901 gtgcagtctt tgcaacaaaa tatttagaaa ctctatcaga aattatggtc taaattcttt
      961 ccaatatttt taagataaga tttttaaatt tgttttttac gacatttgtt gtttcaataa
     1021 cctcagcgtg agaaagtttt aaatcagtaa tgccggcagc ataatttgag atacaactta
     1081 tgcccaaaac gtccattgag cagtaatttg caactatagc ctcaggaacg gtagacatac
     1141 ctgtaacatc agcaccaatg attctgagca tctttacttc ggcaggcgtt tcataactgg
     1201 gaccggcacc tgcagcgtaa actccttttc tgagttttat tttgagttct tttgcacttt
     1261 cttcggcaat ttttataaga tttttcttgt aaatttcact catatcgggg aatcttgggc
     1321 ctaaagtctc gtcatttgac cccaccaaag gattagtccc cattaaatta atatgatcag
     1381 ttataaccat taaatcggca gggttaaagg ctttatttac tgcaccggcg gcattagtta
     1441 taataagggt tttgacaccc agttttttca taactttaac agggtaagtg attttttgca
     1501 tggaatgacc ttcataaaaa tggtatctcc cctgcattat aacaactctt ttaccgcata
     1561 tttgagcaaa aacaagctga cctttgtgtc cttgtacggt agaagtttca aaatcaggaa
     1621 tttcagaata cggaatattt atactttcaa attcggatgc aaaatcgcca agtcccgaac
     1681 ctaaaataat tccgatttcg ggctgaaaat tatttgtttt tttgttgata aaatcaagag
     1741 ttttttgcat aaagccatta tagcagtaaa tggtaaatag taaatagtaa atagaaatgt
     1801 taaaacaggg gacaagaggc gaagatgcca agaggcgtag taggataacc cacgcatcta
     1861 ctcatcttat cttcttataa actattcgac tgttcgactt ttcaactgaa acagcctttt
     1921 agcctaccaa tctgttgtcg tcggcttcgg atgcttttac cataatagcg tgttctacgg
     1981 ggttttcttt tttataagag ctttcgtaat attcgtcaaa tccgaattct tcacgggatt
     2041 tttttgcttg ggtttcgtca accagttttt ttgcgagttc tgctatttca taaaccaatt
     2101 ggtatctgtt ttctgtattt tcgtttaaat cccacgctac tttgattatt tgttccattt
     2161 tttaatcctt tattttcctt gctacataaa taatacaata caaaaattca ttagtcaatg
     2221 ttacatattt tcaatatttc tttgaatgtt atataataaa cttatgaaga aacgacttgt
     2281 ttggatagca gaacttttta tctggctgct cattcttttt gtaatagcag gctctataat
     2341 gtttgcaaga tataactatc gcaaacattt taatacgtat cagatatttc tgcctgatgt
     2401 tgacggcttg attgtaggct ctccggtcag attaatgggg attcaagtcg gatacataaa
     2461 ccagataaat attgtcggcg atgatgttta tgtgaaattt gtcattaccg aaaagaatat
     2521 gaaagtccct caaggctcaa ctgctacagt tgaatttaca ggacttggcg ggtcaaagtc
     2581 tttggaattg tatgcgccta caacagaaaa caaattttta tcggataaat ttataatccc
     2641 tatgccgccg aaaagaattc acgattcttt agggctttta aacgatatgt ttgacaagat
     2701 tatcggaata acttatacta tctctaattt tatggatcaa atcgggatta tgaaatacaa
     2761 caacggaaaa ttgcaaaata atcttacagt aacccaaata ttaaacttaa caagcaagtg
     2821 gattgaggaa gcaaccgaaa aatgcgaaaa ctgcaaaata aaggataagg aaaatggatt
     2881 tacaaaaaac aagagtaata aataaccctg tcattttaca aaatttatgc accatgcggg
     2941 ataaaaatac cgactgtcag ggtgtcagaa ttgcaacaag aaagattaca agagtattgt
     3001 tatatgaggc cgcaaaagga cttcctttga tagaaaaaac tattacaaca ccgctaaccg
     3061 tttttaaggc tcaaaccatt gatcctgata taaatataat aatttcaccg attttgagag
     3121 cgggattaat ttttaccgac gaagcaatag atattcttcc gcaggcttgt ataagacata
     3181 tagggatgta ccgcgatgaa aaaaccctaa agccggtttg gtattataac aaagttccta
     3241 tgaaagtcaa agatcctcat aagttctaca tttttataac agatcctatg cttgcaacgg
     3301 gaaattcgct tttagaagca attaagcttt atgcggacaa aaaaatcccg atagaaaaca
     3361 taaaaataat aagtataatt gcagcacccg aaggacttaa aaatattcat tcaaaatatc
     3421 ccgaagttga aattataact gccgcaatag attcacacct taacgaacac ggatacatag
     3481 ttccgggaat gggcgatgcg ggtgacagaa tttttaacac ttaaagctgt tgtcccagag
     3541 tctcttcaag gtctgtttta ggatcagaaa tttcttttat tccatagacc tcttttattg
     3601 tattaaaaag agtaggatta attatatttg tggcgcattt gcccatgtaa atatgtttta
     3661 tcccgagatg gcttaaataa agcaaattag agattgtatg cttatcgcat ctggttaaaa
     3721 atatactcaa tggcatttta ttcaaaggaa ttttattttt gatttcttta agaattctgt
     3781 aaaataaaac gtaatcatta atcctgtcaa gatgataaac attttctcta tttttattat
     3841 aactgagaga aatagcatag cagtcacttg gcaatagctc aaaaaacgac tcaaaatact
     3901 gcttttgtaa aacaggataa tttaaaagcc caactatata aaggtgtttt atctgtttat
     3961 ttagaatttt atcggttatt tcagccattt tatattctat ttcgtcgtaa gaaaatccga
     4021 ctttaatcgg aggtttttct ataatcttat taaaacctct tgattcaagc gcggctttaa
     4081 tcaaaggctc aaaatcaccg tcaaggattt taataattcc gggcggtgcg accggatcca
     4141 tcgtaaaaag acgccctctg tataaatatt cggacttttc taagttaatc cttgtcatca
     4201 aaattgaacc cggaaaggat gcaaaatcca tcatataact gtcaaaaccc gagccaaaat
     4261 gtcctttaag attcgggtgt gactgcagtt ttggaaatgc atgagcaaaa agcatttcca
     4321 ccccgtgagt atatacccct atttctttac cttcacaagc ttttaaaacc ctttcaagtt
     4381 tattaaaatc agagcctgaa accaaaatcg ctttaccgag ttgtgcgctg gaagctactt
     4441 cggtctgtct tattggaccg taaagctcat tttgagtata aaaaattgtt cttagaatgt
     4501 cataataaat atccaaaaca tcatctatcg cccttttggc ttgctcctgt gaaaaatttg
     4561 tcacattaat tgaagacaac aaagataaaa ttgcataata tccttggtca tggtctttgc
     4621 ccattctttt taattccatt aatttaatac tcatgctttt tattaaaaaa accatgatgt
     4681 caaaaagagc tttttgatta gggtttaata attctgcttt tttgagaaaa tatttttcgc
     4741 cccgctttat tgcatcgcta agataaatat ttttgtgatg cttaaagtaa gttttaattg
     4801 actctatatc caggctgttc ttttcacaaa aatttttata aatggttttt gactccacta
     4861 cataatcgtt taatttatta gtaagttccc taaactgttg ctggttatat tcaacagaca
     4921 atgctaaatt cccgagtgaa taaataacgg tttctttaat ctgctcattt ttaaccccaa
     4981 attcttttaa cttgataaga taaaacgaaa gttcttttaa atacaataaa attatttcct
     5041 gcaaagaaga cagaatggga ctatctgaac aaatccctct gctaaggcat tcgctgcact
     5101 cgttatttgt tttctggaag ttatacataa atacacctca atagcattct acaaataact
     5161 tgaacaaaat aaattacacg ttatgttaat cttttggata attaaagtag aaagcacaga
     5221 aaaattgctt tttacttcaa attattataa aattccttta tgaaaaaagt ttttataata
     5281 tcaccaaaaa gcatcgcagg agagcttatc attgacggtt ttgcctgcgc ttttaaaaat
     5341 ataggctatc gagtgctttt aaaaaaaatt gacgaagtaa cactaaatga gcttgaaaaa
     5401 ttcaatcctg atataatttt cggatatgat tactcgtatc ttacagatca aggttgcaaa
     5461 gaaataatcc aaaaaattca atttaaaaat cgtatttttt atttcggtga cgaacccaaa
     5521 agcaaatttt ctttgggtga aaaaaatgaa ctttacgatg aactttcaaa tttagatgct
     5581 accgtttttg tttgggacaa agcttttaca aaagagttca aaaaatgtta ttatcttcct
     5641 cttgcggtta atcctttaaa atatgcgaca gaattttccg gctataaata tgcaataacc
     5701 tttgttggaa gaccgcttac agataaaaga caaaaaattc tatcttgcct tgtcaaatct
     5761 ttcggaaaca aattaaatat tttctgttac gaaaaacatt ttgaaaaaag cgttgaagaa
     5821 ataaaagaaa aaaaacttct tgaagggcat gagcttgaaa tttataaaaa aagctacaaa
     5881 ggttttgtta aaacagaaag cgaattagct aaaatttaca attcttccga gataaattta
     5941 aacataacag agcagggtaa atcttctttg aactacagag tttttgaggt tttggcatcc
     6001 ggcggctttt tgctgaccga tgaaaaaaag gatgtgcaaa aatactttat accctcaaaa
     6061 cagttggaaa cttataaaaa tgaaaccgat ttaacagata aaatagaatt ttattttcaa
     6121 aacccgggta tcgctcaaag aatagcacat cttggcagat atgaatgtat taaaagccac
     6181 tcttttgatg agaggattaa gacaattatt aaaaaaataa cctaagcttc aacccttgaa
     6241 acgcaagcag aaattgcatc tataagcctt tttgctttaa tgatttctat tcctttaatt
     6301 tgctcaaaat ctttgtttgc aaaaggaata atcgctttct tgaatccgag aatttgagct
     6361 tccttaattc ttttgtctat accttgtact gccctgattt ctccggaaag tcctatttcg
     6421 ccgattatta ccgtgtgaga atcaactacg acatctcttg cgcatgtggc aacggcaagc
     6481 gcaattccca aatccgcagc aggctctgat atttcaatcc cgccgattac gtttacataa
     6541 acatcctgct tagaaaggtt taatccgacc cttttttcaa gtacggcaag aatttgtaat
     6601 acccgcccga agtcaactcc gtttgcaact cttcttggtg aaggataagg cgttgttccc
     6661 acaagtgcct gaatctctac caaaagcggt cttgtgcctt cacttgttga aataattaca
     6721 cttccgggag taacctcttg tgagtgttcc tgaataaaaa ttccgcccgg atttatcact
     6781 tcggctaacc cgtttatccc catttcaaaa attcctgctt cagctgtgtt tccgaaacgg
     6841 ttttttaccg agcgcaaaag cctgtaagac ttgtatttat caccttcaaa ataaacaact
     6901 gtgtcaacca tgtgctctaa tactttaggt cctgcaatgt ttccgtcttt tgtaacatga
     6961 cctatcacaa tcattgttat attctgattt tttgcaattt gtattaaaaa attgcagcac
     7021 tctcttatct gcgaaacact tcctgccgag gaggaaacct cttgagagta aattgcctga
     7081 atactgtcaa caacaacaaa atccggcttt atgttttcta tttctttttt tattaattca
     7141 aaatttgtct ggggataaat gtataaaccg atttcattta caccgagcct ctgtgccctt
     7201 agttttattt gatttgcaga ttcttccgca caaacatata aaaccttttt accctgcctg
     7261 caaagtaaac cgcaggcttg aagcaccagc gtagattttc ctataccggg gtctcccgca
     7321 agcagtgtta cagaaccggt tacaaaacct ccgcccaaaa ctctgtcaaa ttcttttata
     7381 ctgctttgag ttctgatttc ttcgttaatt tctatctcat taatcaatga tggcttattg
     7441 tcaaaaaatt ccgagtttaa aacaacaggc gaaaaatttt gctgaacttc ttcaaccaaa
     7501 cttccccagg cagaacactc gggacatttt cctaaatatc ctgcgctttc ataaccgcac
     7561 tcctgacata tccactttgt tttaactttg gccataaaaa aattatataa caaaaaatca
     7621 ataatgtctt gatgttacaa gcttgggact tgaaataaat atattatcgg gaattataac
     7681 tataggctcg ggtttgtcaa atcttggaat agacgctttc attataaggg tcttgtgata
     7741 aaattcaacc tgagtaacta tcccgttttc aaaagggctt ctacaatcta cccagacact
     7801 gacttgcggg ttgccgttaa cctgattggc cgaaactatt ttataaagag caaaaggctc
     7861 aacatcagcc aaggttccgg tttgcggtga agaaacacta taaccgggat tatttaaagt
     7921 aattgccgaa tctttttttt ctaatatact ctgagcaacg cttacggcag gacaaggcaa
     7981 acccatatta aaatctttta agtcaacaag tgcgacattt gagtttattt cattattgag
     8041 agaataaacc gcgttcacat cctgccaata tagtgcaagt tcaaatatag caa
//
LOCUS       DAOQAU010000065         7850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_11136_length_7850_cov_7.047466, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000065 DAOQAU010000000
VERSION     DAOQAU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11136_length_7850_cov_7.047466"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1367)
                     /locus_tag="PKI94_07930"
     CDS             complement(186..1367)
                     /locus_tag="PKI94_07930"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNW26700.1"
                     /translation="MKRAIIVVIDSMGIGAMPDCKEYNDVFECNTLKNVCEFNSGLNL
                     PNLEKLGLGNIQTFQGLKKVEKPLGKYATLAEKSKGKDTTTGHWEIAGLVLDKPFATF
                     PQGFPNELIHEFIEKTGCGAILGNYPASGTQIIEDLNEKHQKTLYPIVYTSADSVFQI
                     AVDTDMIPLETLYQWCEVARNILDEGGWNVSRVIARPYKIIEGKPTRISKDRRDYSMV
                     PSKPTLLNEIKDSGGKVIAIGKIEDIFAKSGITHAIHIGNNKEGLELTLQAVKEELNL
                     EEIKYKDYSSPLTAHPSLIFTNLVDTDMLYGHRNDAKGYGAALEEIDVYLGEIIKNLK
                     DDDLLIITADHGCDPTVEGTDHTREYVPALFYHKNIIPEDLDLIIGFDYIAKIVKDWL
                     S"
     gene            complement(1369..1689)
                     /locus_tag="PKI94_07935"
     CDS             complement(1369..1689)
                     /locus_tag="PKI94_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26701.1"
                     /translation="MKNQKFDEYIISRLSSDKDFATEYLNSALEEYAKDSDSDALALS
                     IKRLVKAQGSVIDFSKEADINREHLYRIFNAKAVPNLNTLFKIIVSLGFKLEAKPILK
                     EREC"
     gene            complement(1686..2015)
                     /locus_tag="PKI94_07940"
     CDS             complement(1686..2015)
                     /locus_tag="PKI94_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNW26702.1"
                     /translation="MLKKEIEIYTTETGKKPFLEWFEKLDIKSQAKITARLERIEIGH
                     YGDFKKLKESINELRFFAGSGYRIYFAEETTKIIILLTGGDKSSQEKDIKKATEYYKK
                     YKERKQK"
     gene            2121..2780
                     /gene="nusB"
                     /locus_tag="PKI94_07945"
     CDS             2121..2780
                     /gene="nusB"
                     /locus_tag="PKI94_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNW26703.1"
                     /translation="MQARRAARELALILFSQFDNKITNYSKADFEEIILKSVRILTNN
                     ASEDLKLTLGALFNIKEFIENYEADHETNLQRPLEVSNLPVPVPMTSDMTGRIDEMIE
                     IAEKALLALEIAEFATLESQNDVKNYAILIAEQFKEHHDFVDAQIQKFAKGWDIQRLV
                     KMDKDILRIAIIELLYIKDAPMKVIVDEALELAKKYSTDDSSSFINGILAKVIVENGV
                     G"
     gene            2904..3821
                     /gene="ftsY"
                     /locus_tag="PKI94_07950"
     CDS             2904..3821
                     /gene="ftsY"
                     /locus_tag="PKI94_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNW26704.1"
                     /translation="MFNFFGLKLDKLKETVSETAHTLVDNVVNAVEEEAEFSEFVLDD
                     MEDLLIRADLGVNYASELVDKLRNQDRIKPSAVKNYLRGEFLNALHFAGDTKINYKEN
                     SLNIYFISGVNGVGKTTLIGKLAYRFKQEGKKVLIAAGDTFRAAAEEQLDIWSKRADA
                     DIVRRDKADPASVVFEAIQKAKDENYDIILVDTAGRLQNKFNLMEELAKIKAVIDKNA
                     PESLRECILVLDANTGQNGLQQAKVFQEAVNLTSVALTKLDGSAKGAIVLAVAKELKL
                     PVKLIGVGEKMEDLKDFNPDDFIEALFAK"
     gene            3832..4503
                     /locus_tag="PKI94_07955"
     CDS             3832..4503
                     /locus_tag="PKI94_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNW26705.1"
                     /translation="MENIEILKRTLSKNGHEIILVEQAKKENIPLTAHGSQLTVLIIG
                     VVHGDEPQGKFLIENYLQNTESKSKNRLLFIPCLNPDGLENNTRSNSNGVDINRNFPT
                     KNWGGGDCEVTSNFYGGESPASEIETKFILEIMKEYSPDVILTLHSPYRVVNYDGPAK
                     ETAEKIGQIMGYPVSSDIGYSTPGSFGTYAGVERNIPIITLELDDKISIYNLILPIKQ
                     LFEEI"
     gene            complement(4471..4983)
                     /locus_tag="PKI94_07960"
     CDS             complement(4471..4983)
                     /locus_tag="PKI94_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26706.1"
                     /translation="MEIRPRKVQTNVIFHDVDHGINKNYNMPVGSKIDVNGLTYNVKE
                     NGTYYKGKRVNQINIHEDERLCLSTFNSTQDSVIDRNDTRVFVNGRHRNYVDDANRRL
                     NEEGADCRIKKTQDEDGSVIDGSCDENGFGVYLTNNRTNNHRLFGVTFPSASNQGSPI
                     SNFFKKLFNR"
     gene            5216..5458
                     /locus_tag="PKI94_07965"
     CDS             5216..5458
                     /locus_tag="PKI94_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HNW26707.1"
                     /translation="MAQEPKIIATIPRSATEQLQISINEYKGKSYLDMRIFYTTDDGA
                     NWLPTKKGVTCSPENIEVLKDAIDEAMKEFMSIEEE"
     gene            5459..7708
                     /gene="priA"
                     /locus_tag="PKI94_07970"
     CDS             5459..7708
                     /gene="priA"
                     /locus_tag="PKI94_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNW26708.1"
                     /translation="MGVLNKYALALVNIKGLGVKTFSYLIPDEMKDIIQIGQAILVPF
                     GRQGNVNAFVVGFGDYLPEGIKAKKISKILDEKPLFTLEYLKLLEWIANYYVCDFVTV
                     LNAAVPMRLLEGNSKTEKNIEYVEGILPRPLREKAGVRGISNRQLEILSALKEKGKMP
                     LIEFEKLAKTTRATIIKLEKLGCVKISDEEIYRNPLENLGVSKKEELFKLSPQQEKVY
                     QGINKKIHKSKTPILIHGITASGKTEIYFKLIDDTIKQGKNVLFLAPEIALASQLTKR
                     LAKKFGTKDVAIWHSSISEGERYDVWQKLYKDEIKILAGVRSAVFAPLKNIGLIIIDE
                     EHESAYKQTTPAPRYDARTIAFKLAEFHNAPLLLGSATPDVGVYYKASNSDNLFELKN
                     RFNDAPMAKTSVINMQNNAKQAYKGIISVQLQKAIEETLEKGQQVILLINRRGFSTYT
                     QCQSCGDVIECENCSIPMIWHSQDLTLKCHYCGRTKSFPEQCEKCGSDTIKNSGMGTQ
                     KIELLIKERFKNARTERIDSDILVKKNEHIKLLERFQKGEIDILIGTQMIAKGLDNPN
                     VTLVGVISADSSFAMPDFRASERGFQLLTQVAGRSGRGEFSGSVIFQTYNPDFYAFQS
                     AKEQNYNEFYETEIKAREEFDYPPFSQVIRLILSGENNFRAEKSAQEIALRLNTIIDK
                     QGISERLIVLGPSPCVIEKIGGKYRFQIIIKNKMEEKGHMFISKFLSQITMPKDIKMT
                     IDIDPIDIL"
BASE COUNT         2594 a         1380 c         1419 g         2457 t
ORIGIN      
        1 gatttacttt tacaacatct tccacagaaa atactgcttc acaaattgat tcacatgcgc
       61 cacaagcaat gcatccgtct tcgattttta cattcatttt aatttctcct tatttctaat
      121 atatgaaaaa gtaaacaaaa agtttactaa atcgattata gcaagaacaa attaaaaatc
      181 cagtgttaag ataaccaatc ttttacaatt tttgcaatgt agtcaaaacc tattatcaaa
      241 tccaaatctt cagggattat atttttatga tagaaaagtg cagggacgta ctcgcgagtg
      301 tgatctgttc cttcaactgt gggatcacag ccgtgatcag cggttattat aagtaaatca
      361 tcgtctttta aatttttaat tatttcgccc aaatagacat caatttcttc cagtgccgct
      421 ccgtagcctt ttgcatcgtt tctgtgccca tagagcatat ctgtgtcaac aagatttgtg
      481 aaaatgagtg aagggtgagc ggtgagcggt gaactgtagt ctttgtattt aatctcttcc
      541 aagttcaact cttctttaac cgcctgaagc gttaattcca aaccttcttt gttgtttcct
      601 atgtgaatag catgagttat tcccgatttt gcaaaaatgt cttcaatctt tcctattgca
      661 atgacttttc cgccagagtc tttaatttca ttgagcaatg tgggcttaga aggcaccatg
      721 gaataatctc ttctgtcttt ggaaattctt gtaggcttac cctcaattat tttataaggt
      781 cttgctataa cccttgaaac attccacccg ccctcatcca agatatttct tgcaacttca
      841 caccattgat aaagggtttc aagcggtatc atatctgtgt caacagctat ttgaaaaacg
      901 ctgtcggctg aagtataaac aatcggatag agcgtttttt ggtgtttttc atttaaatct
      961 tcaataattt gtgtaccgct ggccggataa ttccccaaaa ttgctccgca gcctgttttt
     1021 tcgataaatt cgtggatgag ttcgtttgga aagccttgtg ggaacgtcgc aaaaggtttg
     1081 tcaagaacaa gccctgctat ttcccagtgt cctgttgtag tatctttgcc tttggatttt
     1141 tcggcaagtg ttgcatattt accgagtggt ttttcgactt tcttcaaacc ttgaaaagtt
     1201 tgaatattgc caagcccaag cttttccaaa tttggcaaat tcagtccgct gttaaattcg
     1261 cagacatttt tgagcgtatt gcattcaaaa acgtcattgt actccttgca atcgggcatt
     1321 gcacctattc ccattgaatc tatgacgact ataattgctc gtttcatttt agcattctct
     1381 ctcttttagt ataggcttgg cttccaattt aaaacctaaa cttacaataa tcttaaataa
     1441 agtattcaga ttcggaacag ctttggcgtt aaaaattcta taaagatgtt ctctgttaat
     1501 gtcggcttct ttggaaaaat caataacact gccttgagct ttaacaagcc tttttattga
     1561 aagtgcaaga gcatcggaat ctgagtcttt tgcatactct tccaatgcac tgttaaggta
     1621 ttcggtagca aaatccttat cagacgacag ccttgaaatt atatattcat caaatttttg
     1681 atttttcatt tttgtttcct ttccttgtat tttttatagt attcagttgc ttttttaata
     1741 tctttttctt gagatgattt atctccgcct gtcaacaaga taattatttt tgtggtttct
     1801 tcggcaaaat aaatcctgta ccccgaacct gcaaaaaatc taagctcatt aattgattct
     1861 ttaagttttt taaaatcgcc gtaatgccct atttcaattc tttcaagtct tgccgttatt
     1921 tttgcttgac tttttatatc aagcttttca aaccattcta aaaaaggttt tttacccgtt
     1981 tcggttgtat aaatttcaat ttcttttttt aacataaata tattgtagcg taaaagctac
     2041 attatgtcaa gtatttcgaa ttttacattt gccttatttg tgatttaatt gtagtacaga
     2101 ttacaaatga gggcagaatc atgcaggcac gaagagcggc gcgggaattg gctttaatac
     2161 ttttttcgca gtttgataac aaaattacaa attattcaaa agccgatttt gaagaaatta
     2221 tacttaaatc ggtaagaatt ctcacaaata acgcaagcga agatttaaag ctgactttag
     2281 gcgcgttgtt taatataaaa gaatttatag aaaattacga agcggatcac gaaacaaacc
     2341 tgcaaagacc tttggaagtt tcaaatctac ctgtgcctgt tcctatgaca tctgatatga
     2401 caggaagaat tgacgagatg attgaaattg cagaaaaagc tttgcttgcg cttgaaatag
     2461 cagaatttgc aacgcttgaa agccaaaatg acgtaaaaaa ttacgcgatt ttaatagcag
     2521 agcaattcaa agaacatcac gactttgttg acgcccagat tcaaaaattt gccaagggct
     2581 gggatattca gagacttgtc aaaatggaca aagatatttt acgaatcgcc ataattgaac
     2641 ttttgtacat aaaagacgca cctatgaaag ttattgttga tgaggcactt gaacttgcaa
     2701 aaaaatattc cacagacgac agttcgtcat ttattaacgg gattttagct aaagttattg
     2761 tcgaaaacgg ggtgggttaa ggttttcgtc ttttacgtct ttgcgaggag cgaagcgacg
     2821 cggcaatcta agcacttttc tacgtttttt ggattgcttc accctaaagg gttcgcaacg
     2881 acaaattaaa aataggaaaa tgaatgttta attttttcgg actaaaactt gataaactta
     2941 aagaaaccgt atcagaaacc gcacacacat tggttgataa tgttgttaat gcagtcgaag
     3001 aagaagctga attttccgaa tttgtgctcg atgatatgga agatttgctc atcagagccg
     3061 atttgggagt aaattatgca tctgaacttg ttgacaaact tagaaaccaa gacagaatta
     3121 agccttctgc ggtgaaaaat tatcttagag gagaattttt gaatgcgctt catttcgcag
     3181 gcgatactaa gattaattat aaagaaaatt cattaaatat ttattttatc tcaggtgtaa
     3241 acggcgtggg aaaaaccact ttgataggta aattggctta tcgttttaag caggaaggca
     3301 aaaaagtttt aatcgcagca ggcgacacat tccgcgcagc agcagaagaa cagcttgaca
     3361 tttggtcaaa aagagcagac gctgatattg tccgcaggga taaagcagat cctgcttctg
     3421 tagtttttga agctatacaa aaagcaaaag acgaaaatta tgatataatt cttgttgata
     3481 ccgcaggacg tttgcaaaat aagtttaatc ttatggaaga gcttgcaaaa atcaaagcgg
     3541 taatcgacaa aaatgcacct gaaagtttgc gcgaatgtat tttagtactt gatgcaaaca
     3601 caggacaaaa cggacttcaa caggcaaaag tttttcaaga agcggtgaat ttgacatcgg
     3661 ttgcgcttac aaaacttgac ggctctgcaa aaggtgcaat agtcttagcg gtggcaaaag
     3721 agcttaaatt acccgttaaa ctgataggcg tcggtgaaaa aatggaagat ttaaaagatt
     3781 ttaatccgga tgactttatt gaagctttgt ttgcaaaata aggagtaaaa aatggaaaac
     3841 attgaaatat taaaaagaac actatccaaa aacggacatg aaataatatt ggttgagcaa
     3901 gccaaaaaag agaatattcc gctcacggct cacggttcac aactcaccgt tctaataata
     3961 ggtgttgtcc acggagatga accccaaggt aaatttttaa ttgaaaatta tctgcaaaat
     4021 accgaatcta aatctaaaaa tcgtttgctt ttcattccct gtcttaatcc tgacgggctt
     4081 gaaaataata cacgttcaaa ctctaacgga gttgatatta acagaaattt tccgacaaaa
     4141 aattggggcg gcggtgattg tgaagtaact tcaaatttct acggcggtga aagccctgcc
     4201 tctgaaatag aaacaaaatt tatattagaa atcatgaaag aatattcacc tgatgtaatt
     4261 ttaacgcttc actcccctta cagggttgta aattacgatg ggcccgcaaa agaaacggca
     4321 gaaaaaatcg gacagattat ggggtatccc gtttcctccg atataggcta ttcaactccg
     4381 ggcagttttg gaacttacgc cggagttgaa agaaatattc ctataattac tcttgaattg
     4441 gatgataaaa tcagtattta taatttaatt ttacctatta aacaactttt tgaagaaatt
     4501 tgatataggt gaaccttgat tagatgcaga aggaaaagtc actccaaata atctatgatt
     4561 atttgttcta ttatttgtaa gatatacgcc gaatccgttt tcatcacagc ttccgtcaat
     4621 aactgaaccg tcttcatctt gtgttttctt tattctgcag tcggctcctt cttcattaag
     4681 ccttctatta gcatcatcga cataatttct atgtcttccg ttcacgaaaa ctcttgtgtc
     4741 atttctgtca ataacactat cctgagttga attaaatgtg ctcaaacaca gcctctcatc
     4801 ttcatgtata tttatttggt ttactctttt acctttataa taagttccgt tttctttgac
     4861 attataagtt aaaccattaa cgtcaatttt tgaaccaacc ggcatattat agtttttatt
     4921 tattccgtgg tcaacatcgt gaaagataac atttgtttgt acttttcttg gtctgatttc
     4981 catttttaat cctccctaaa taattaaata tcctctatat acttttaaag ttttttttat
     5041 aacaaaaatt gcttataaat tctaaaacat tatttatcta aacttataaa gcagcaaaaa
     5101 caaatatttt aatcacttta catttgttaa caaaattttt tattgcttta taaaagtttt
     5161 tatttttaat ttatgttata cttttttcgt ataaaaattt aagaaggaga gagaaatggc
     5221 gcaagaacca aaaattatag ctacaatacc tagaagtgcg acggaacaat tacaaatttc
     5281 aataaacgag tacaagggaa aaagttacct tgacatgaga attttttaca caactgatga
     5341 cggtgcaaat tggcttccga caaaaaaagg tgtaacttgt tctcccgaaa atatcgaagt
     5401 tttaaaagat gcaatagatg aagcaatgaa agagttcatg agcattgaag aggaataaat
     5461 gggtgtattg aacaaatacg ctcttgctct tgttaatatc aaagggcttg gggtaaaaac
     5521 tttcagttat ttgattcccg atgagatgaa agatataatt caaatcgggc aggcaattct
     5581 tgtgcctttt ggcaggcaag gaaatgtaaa tgcttttgta gttggctttg gtgattattt
     5641 gcctgaaggt ataaaagcaa aaaaaataag caaaattctt gatgaaaaac cgctttttac
     5701 ccttgaatat ctaaaactgc ttgaatggat tgcaaattac tatgtgtgtg attttgttac
     5761 agtcttaaat gccgctgttc ctatgcgatt gcttgaagga aattcaaaaa cagaaaaaaa
     5821 tattgaatat gttgaaggga ttctccctcg ccccttgcgg gagaaggctg gggtgagggg
     5881 tatttcaaat cgtcagcttg aaattttaag cgcgctcaaa gaaaaaggca aaatgccttt
     5941 aatcgagttt gaaaaacttg caaaaaccac aagagctaca ataatcaagc ttgaaaagct
     6001 tggttgtgtg aagatttccg atgaagaaat ttacagaaat ccgcttgaga atctcggggt
     6061 ttccaaaaaa gaagaacttt ttaagctttc acctcagcag gaaaaggtct atcagggcat
     6121 aaataaaaaa atacacaaaa gcaaaacgcc gattcttatt cacggaatta ctgcatcggg
     6181 caaaacggaa atttatttta aattaatcga cgatacgata aagcaaggaa aaaatgtttt
     6241 gtttttagct cctgaaatag cactcgcctc gcagcttaca aaaaggcttg caaagaaatt
     6301 cggtacaaaa gatgtcgcaa tctggcacag cagtatctcc gaaggtgagc gttacgatgt
     6361 ttggcaaaaa ctatacaaag atgaaataaa aattctggca ggtgtgcgct ctgccgtttt
     6421 tgctccgctt aagaatatcg ggcttataat tatagacgaa gaacacgaaa gtgcttataa
     6481 gcaaacaaca cccgctccaa gatatgacgc aagaacaatc gcatttaagc ttgcagaatt
     6541 tcacaatgcg cccctgcttt taggaagcgc aactcctgat gtgggagttt attacaaggc
     6601 atcaaattcc gataatcttt ttgaacttaa aaacagattt aacgatgcac ctatggcaaa
     6661 aacttcggtt ataaacatgc aaaacaatgc caagcaggca tataaaggta taatttccgt
     6721 tcagcttcaa aaagccattg aagaaacgct tgaaaaagga caacaggtaa ttttgcttat
     6781 aaacaggcgc gggttttcta catataccca atgtcaatcc tgcggagatg taatcgaatg
     6841 tgaaaactgt tctatcccga tgatttggca cagtcaagat ttaaccctaa agtgccacta
     6901 ctgcggcaga acaaaatctt ttccggaaca atgcgaaaaa tgcggaagcg atacgataaa
     6961 aaattcagga atgggaactc aaaaaatcga acttttgatt aaagaacggt ttaaaaacgc
     7021 aaggacagag cgcattgaca gcgatattct ggttaaaaag aatgaacaca taaaacttct
     7081 ggaacgtttt caaaaaggcg aaattgatat tcttatcggt actcaaatga ttgcaaaagg
     7141 attggacaat cccaatgtta cgcttgtggg ggtaatttct gcggattcaa gctttgctat
     7201 gcctgatttc agggcatctg aaagaggctt ccagctttta acccaggtgg caggacgctc
     7261 cggcaggggg gaattttccg gaagcgtaat tttccaaact tacaaccctg atttttatgc
     7321 ttttcaaagt gcaaaggaac agaattacaa tgaattttac gaaacagaaa ttaaagcgcg
     7381 tgaagaattt gattatccac ctttcagcca agttataagg cttattttga gcggagaaaa
     7441 taatttcagg gcggaaaaat ccgcacagga aattgccctg agactaaaca cgataatcga
     7501 caaacaagga atatcagaaa ggcttattgt acttggccct tcgccttgtg ttattgaaaa
     7561 aataggcgga aagtaccgct tccagattat tattaaaaat aaaatggaag aaaaagggca
     7621 tatgtttata tcaaaatttc tttctcaaat cacaatgcca aaagacatca aaatgactat
     7681 agatattgat cctattgaca tattgtaaaa aattttacct atttactaat cctccccaga
     7741 tgacctattt ttgtatcttt cctgtaacat tttgttaaaa aataacaatt tttttctttt
     7801 agatttatta tatagaggac aaggaaaagt caaaaatata caagtgttaa
//
LOCUS       DAOQAU010000066         7640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_11569_length_7640_cov_6.068425, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000066 DAOQAU010000000
VERSION     DAOQAU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11569_length_7640_cov_6.068425"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2132
                     /locus_tag="PKI94_07975"
     CDS             <1..2132
                     /locus_tag="PKI94_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HNW26709.1"
                     /translation="ETPFKASNQAEREGILAKSKLFSPVLAAVFLSFWLQKAALSVTN
                     INVEKNFPDYSCEFCGKDKCERFNRKLFLFNLKLNKYLIKPVDVVWSSILPKYGMERL
                     QNAYNNINFPVRVTSCLLQKDFHASKQEVARFFINTTMGVGGLWDPAKTKFKIEANQE
                     DIAQVLYHYKVKQGPYLVLPIVHGNVRTVVGKILDCPFNPCSYVLGPFAIIASTVFFI
                     NNSACMQPAIKILEQTCADPYEVLRQIDGVESFIKNNNLDRSNIFLEKIASGNKNLVS
                     AAKIDDGNVNVRLTPPVSADIELKNFNPQSPLVDAMRTVLLDKENPKKSKWAQTSVWN
                     NDFEKKIKKASIRFDNKLVKYTYRYILQKDKNAPAAIIYPAIGEGVMAEHSTTMTRIL
                     YDQGYSVIVLGSPFNWEFIKSMPDNYKPGLPSKDTDLTRLLTLKIINRLEAQYKLHFS
                     DKIIVGSSFGALVTLFTAAKEEKENILGVSKFIAINPPIELMFAQRQIDKYAQDWKNS
                     PSDIKMRAAVTVQKVIKKTEEIAAQKPTESVYLPFDDDEAKLIAGFVMKQKLADTVFS
                     VEKCSRCKKDLIHDEVNNMTFDEYAQKYVLAGEKKPYEQIDYESSLYSISDYLKNNDN
                     YKIYHTVDDYFVNSQQLAWLKNQAQDKALYFSNGSHLGYLYRREFLDDFKGEIKLKNS
                     KPQQEKRNLFVKILKPDLEKTDLSLAQ"
     gene            2209..2925
                     /locus_tag="PKI94_07980"
     CDS             2209..2925
                     /locus_tag="PKI94_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26710.1"
                     /translation="MKKMFQKIVATFLLFVMATFNAAFAQETINAPEQNMPLKDSVEI
                     SKVKVIIPKNSKINLAFAQKFNANKAQVGDKVNFILSENLLSNDGKIALPKGTEFIGV
                     VENQVKAKSFNRNARALIVIKQAKLPSGEMINLNANVNKKKGELKSPFYANLPKLILE
                     VGVVAGIGMAAMAIVIGYAIASVFTGGIAAIALLPVVTGAAAGLGVIVCTTSKGVNYK
                     ASAGKVIPFVLQNDLELYLN"
     gene            complement(2945..3511)
                     /locus_tag="PKI94_07985"
     CDS             complement(2945..3511)
                     /locus_tag="PKI94_07985"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HNW26711.1"
                     /translation="MNGLSIVSGLAEYLAANEYIAGVPKIGEQGCCSGVSSIFGSNDD
                     INDQALISDEATSLFEAEKTLQTSSGLTPEEQKEVERLKETDREVRAHEQAHIAASAG
                     ISTSSPHYTYVTGPDGQRYAVEGKVDVSFKESSDPQETIRRAQAMKSAATAPSDPSGQ
                     DFAVAAQAQQVIDKAEQELSQQNDKSSS"
     gene            3671..4396
                     /locus_tag="PKI94_07990"
     CDS             3671..4396
                     /locus_tag="PKI94_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26712.1"
                     /translation="MYKVCSLDYINSEIFDVDILSADEKILYPAGTKITPEILLKLYF
                     KEIYADEKTKIEQLDILPQEQVVEIENNTRTDEYNFDVDEKGYIIFDEQQAMRVSDLA
                     LSIGKEIKYNKEQLEILKQGAYYHNIGITNFKPEDLRKKDFKQLQAQAGYEILKNQKN
                     MPEEIALCAKLWINNYEPSAHNLKEDIPYHHVVAISRFYDKLGTQKIQKEKILEIMLK
                     CGGNKFNIYVLHKFIRMMRGQNG"
     gene            4389..5264
                     /locus_tag="PKI94_07995"
     CDS             4389..5264
                     /locus_tag="PKI94_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNW26713.1"
                     /translation="MANFQKEQLNEFFQFFNDTVKKALLNNLDKEVCKNIDLQIESNM
                     KVQDVESLKNNQALYQLEYVLGELQGSLIVTLPENLIFHLADILMCGGGKKDYSGVLS
                     EIETNSFKKLLEQVFKALEKTFKVKYDKNLVFSEKASLTLKEDDKYQTIFSGLGLDFQ
                     VVEKITINDSEEYIFNLLLNYATLEELMNLSGINYTEVDSKKINVNATDLKRLSDVKI
                     NITAELGRAQIPIKYALELVRGSVVELDTLNNSDIKVFVNGSEFAKAQIFAVEDNYGL
                     KITEIISPEERMQSL"
     gene            5261..6175
                     /locus_tag="PKI94_08000"
     CDS             5261..6175
                     /locus_tag="PKI94_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW26714.1"
                     /translation="MISSIFLILIIFSILFIVAWIWFFGVNSSKSINGIYNRALKKME
                     KEKYKEAKDLFIIVEAQDSNYKELQKYLGICYVKLENYEKAKDYFEKLIKTASKDFLV
                     LQNAATVFFNLKDFQRAEEFYKKALEINKANADCFMNLGISQFCQNKYQDAIESLKDY
                     EFKFGANVKTQFYINRCEESICDENDKQKMSEILNEYVRLSKLPEFPREFDNVLAVFF
                     AKMGKIDLMLESCNKIISYNPEDTNAYKLLGLVNVFMKKPAQAKNDLSIALDLDPENE
                     EVKEILSFILCQEEKHMQSKECREKVRN"
     gene            6270..6413
                     /locus_tag="PKI94_08005"
     CDS             6270..6413
                     /locus_tag="PKI94_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26715.1"
                     /translation="MSKKVLVIDDSPFVFKAVSKALEGTEWEVVGNALDGQLGIDKYL
                     EMS"
     gene            6489..6770
                     /locus_tag="PKI94_08010"
     CDS             6489..6770
                     /locus_tag="PKI94_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW26716.1"
                     /translation="MQKGIKAKPEEIISILRQIEILTNQGNSIQMSCREAGITEQTFY
                     RWRKQYGGMQLPHIKEMKKLQEENVRLKKIVADLTLEKLILNEVVQGKF"
     gene            complement(6976..>7640)
                     /locus_tag="PKI94_08015"
     CDS             complement(6976..>7640)
                     /locus_tag="PKI94_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNW26717.1"
                     /translation="LRAEGWRVNHKRVERIWRKEGLRVPQKQKKRGRLYFNDGSCIRL
                     RPLHKNHVWSYDFVQDKLYNGKKIRMLTILDEFTRKCLRIEVNYSLKSNDLIEVLSEL
                     FITKGVPEYIRSDNGSEFVAKNIQAFLRQIRVKTAYITPGSPWENGFNERFNGILRDE
                     LLNGEVFYTLKEAKVMAENYRREYNEIRPHSSLNYAPPAPVTKLLNAEILTLNLVQKL
                     GA"
BASE COUNT         2745 a         1259 c         1381 g         2255 t
ORIGIN      
        1 ccgaaacgcc gtttaaagcg agcaaccaag cagaaagaga ggggattttg gcaaaaagta
       61 aattattttc gcctgtttta gcagctgttt ttttaagttt ctggttacaa aaagcagcac
      121 tgtcggttac aaatataaat gttgaaaaaa actttccgga ttattcttgt gagttttgcg
      181 gaaaggataa gtgcgaaagg tttaaccgta aacttttctt gtttaacctc aaattaaaca
      241 aatatttgat aaaaccagtt gatgttgttt ggtcatcgat tttacccaaa tacggaatgg
      301 aaagacttca aaatgcttat aacaacataa atttcccggt aagagttaca agctgtcttt
      361 tacaaaaaga ttttcacgcc tcaaaacaag aagtagcaag gttttttata aatacaacaa
      421 tgggtgttgg cggtctttgg gatcctgcaa aaacaaaatt taaaattgaa gctaaccagg
      481 aagatattgc ccaagtttta taccactaca aagtcaaaca aggtccttac cttgtgcttc
      541 cgattgtgca cggtaacgtg agaactgttg tcggcaagat tcttgactgc ccgttcaatc
      601 cttgctctta tgtgcttgga ccttttgcaa taattgcatc cacagtcttt ttcataaaca
      661 attccgcctg catgcagcct gcaataaaaa tcttagagca aacctgcgct gacccttacg
      721 aagttttaag acaaatcgac ggcgtagaaa gttttattaa aaacaataat ctggacagaa
      781 gcaacatttt tcttgaaaaa atagcttcag gcaacaaaaa tcttgtcagc gcggcaaaaa
      841 ttgacgacgg taatgttaac gtaagattaa caccgcctgt ttctgcagat atagaattaa
      901 aaaacttcaa ccctcaaagc cctttagtag atgctatgcg aacagtttta cttgacaaag
      961 aaaaccccaa aaaatcaaaa tgggcacaga cctctgtttg gaacaatgat tttgagaaaa
     1021 aaattaaaaa agcgtcaata agatttgaca acaagcttgt gaaatatact tacagataca
     1081 ttctgcaaaa agataaaaat gcacctgctg caataattta tcctgcaatc ggagaaggtg
     1141 taatggcgga acattcaaca actatgacaa gaattttata cgatcagggt tattctgtaa
     1201 ttgttcttgg aagccctttt aactgggaat tcattaaaag tatgcccgat aattataagc
     1261 ccggattgcc ttcaaaagat accgatttaa caagactttt aactctaaaa atcatcaatc
     1321 ggcttgaagc tcaatataaa ttacacttta gcgataaaat tattgtcgga agctctttcg
     1381 gggctttggt tacattattt acggcggcaa aagaagaaaa agaaaatatc ttgggcgttt
     1441 caaaatttat agcaataaat ccgcctattg aacttatgtt tgcgcaaaga caaatcgata
     1501 aatacgctca ggattggaaa aatagccctt ccgacataaa aatgagagca gccgttacgg
     1561 ttcaaaaagt cattaaaaaa acagaagaaa tcgcggcaca aaagcctaca gaatcggtat
     1621 atttgccctt tgacgatgat gaagcaaagc tcattgcagg ttttgtaatg aaacaaaagc
     1681 ttgcagatac tgttttttcg gtggaaaaat gttcaagatg caaaaaggat ttaattcatg
     1741 atgaagtaaa caatatgact tttgatgaat atgctcaaaa atatgttttg gcaggtgaga
     1801 aaaaacctta tgagcagatt gattatgagt caagtttata ttctatttcc gattacttaa
     1861 aaaataatga taattacaaa atttatcaca cagttgacga ttatttcgta aattctcaac
     1921 agcttgcatg gcttaaaaat caggcgcagg ataaagccct ttatttcagc aacggctctc
     1981 atttggggta tttgtacagg agggaatttc ttgacgattt taaaggtgaa attaaattga
     2041 aaaattcaaa acctcaacaa gaaaaaagaa atttattcgt aaaaatttta aaaccggatt
     2101 tagaaaaaac ggatttatcg cttgcgcagt aaaattattg ctttaattct atttaaatgc
     2161 tattataaat gcattctgtt gacactgaat taaagaattg gagtaagtat gaaaaaaatg
     2221 tttcaaaaaa ttgtagcaac ttttttgtta tttgttatgg cgacttttaa tgccgctttt
     2281 gcgcaggaga cgattaatgc tcctgaacaa aatatgccgc tgaaagattc tgttgagatt
     2341 tcaaaagtaa aagttattat cccaaaaaat tcaaaaataa accttgcttt tgcccaaaaa
     2401 tttaacgcaa acaaagcaca ggtcggagat aaggtcaatt ttattctttc ggaaaattta
     2461 ctctcaaatg acggaaaaat agctttgccg aaaggaaccg agtttatcgg agtggtggaa
     2521 aatcaagtca aagcaaaatc ttttaacaga aacgcaagag cacttattgt aataaaacag
     2581 gcgaaacttc cctcaggtga aatgataaat cttaatgcta atgttaataa aaagaaaggt
     2641 gaattaaaat caccatttta tgcaaacttg cccaaattaa tacttgaagt cggagtagta
     2701 gcaggtatcg gaatggcggc tatggcaata gttatcggtt atgctattgc atctgtattc
     2761 acaggtggaa tagcggcaat agcactgtta cctgttgtaa caggtgcagc agcggggctc
     2821 ggagtaatcg tttgtacaac aagtaaaggc gtaaattaca aggcatctgc aggcaaagta
     2881 attccatttg tgttacaaaa tgatttggaa ctttatttga attaaaaata aatatgctta
     2941 aaaattaaga tgaagattta tcgttttgtt gggataattc ttgttctgct ttatcgatta
     3001 cctgctgagc ttgtgctgca accgcaaaat cctgtcctga ggggtcagac ggtgcagtag
     3061 ctgcggattt catagcttgg gctctgcgta tagtttcttg agggtcagaa ctttctttaa
     3121 agcttacatc cactttgccc tcaacagcat atctttgacc gtcaggccct gttacgtagg
     3181 tgtaatgggg cgaagatgtg cttatgccgg cagaggcggc aatatgtgcc tgttcatggg
     3241 ctcgaacttc gcggtcggtt tctttaagcc tctcaacttc tttttgttct tcgggagtca
     3301 atcccgaact tgtttgcaag gttttttccg cttcaaataa gcttgttgct tcatcagaaa
     3361 tcaaagcttg gtcatttatg tcgtcatttg agccaaaaat tgacgaaaca cctgaacaac
     3421 agccctgttc tccgattttc ggcacaccgg caatatattc gttcgcggct aaatattcag
     3481 ctaaacctga tacaatactc aatccattca ttttctaata ttaacacttt taaaaacgtt
     3541 tttcaagctt acaaaaatta atttttctaa aaaataaata aatcaaaaaa catgctataa
     3601 tatattttga agataataat gtcatgctgt ggttacgctg aaatcaggtg atttattaga
     3661 aaagggtgtt atgtataaag tttgttcact ggattacata aatagtgaaa tatttgatgt
     3721 tgatattttg tcggcagacg aaaagatatt ataccctgcc ggtacaaaaa taacccctga
     3781 aattctttta aaactttatt ttaaagaaat ttatgccgat gaaaaaacca aaatcgaaca
     3841 acttgatatc ttaccgcaag aacaggtagt tgaaattgaa aacaacacaa gaactgacga
     3901 atacaacttt gatgttgatg agaaaggtta tataattttt gacgagcagc aagcgatgcg
     3961 cgtttcagat ttagccctaa gcataggaaa agaaattaaa tacaacaaag aacagcttga
     4021 aatccttaag cagggtgctt attatcacaa tatcggcata acaaatttta agcccgaaga
     4081 tttaagaaaa aaagatttca agcaattaca agcacaggca ggctatgaaa ttttaaaaaa
     4141 tcaaaagaat atgcccgaag agatcgcttt atgcgcaaaa ttatggataa acaattatga
     4201 accgtcagca cataacttaa aagaagatat tccataccat catgtagtcg caataagccg
     4261 cttttacgac aaattaggaa cacaaaagat acaaaaagaa aaaattttgg aaatcatgtt
     4321 aaaatgtggt ggaaataaat ttaatatata tgtgttacat aaatttataa gaatgatgag
     4381 aggacaaaat ggctaacttt caaaaagagc aattaaacga attttttcaa ttttttaacg
     4441 acacagttaa aaaagcacta ttgaacaatc ttgataagga agtctgtaaa aatattgatt
     4501 tacaaataga atcaaatatg aaagttcaag atgtcgaaag tttaaaaaac aatcaggcat
     4561 tgtaccaatt agaatacgtt ttaggagaac ttcaagggag cttgatagta acattaccgg
     4621 aaaatttaat ttttcatctt gcagacatac ttatgtgcgg cggcggcaaa aaagattata
     4681 gcggagtact cagtgaaatc gaaacaaact cttttaaaaa acttcttgaa caagttttta
     4741 aagctcttga aaaaaccttc aaggtaaaat acgacaaaaa tctcgtcttt agtgaaaaag
     4801 cctcactgac tttaaaagaa gatgacaaat accaaacaat tttttcaggt ttaggtttag
     4861 actttcaagt tgttgaaaaa ataacaataa acgactctga agaatatatt tttaacctgt
     4921 tgctgaatta tgcaacgctg gaagaattaa tgaacttgtc aggtataaat tatacagaag
     4981 ttgattcaaa aaaaatcaac gttaacgcca cagacctaaa aagactttct gatgtaaaaa
     5041 taaacataac tgcagaattg gggagagcac aaattcctat aaaatacgct ctggaacttg
     5101 tcagaggttc tgttgtagaa ttggatacgt taaacaattc tgacatcaaa gtttttgtca
     5161 acggttctga atttgcaaaa gcacaaattt ttgcagttga agataattac ggactaaaaa
     5221 taactgaaat tatctcgcct gaagaaagga tgcaaagtct ttgataagtt caatattctt
     5281 aattttaata attttttcaa tcctttttat tgttgcttgg atttggtttt ttggtgtaaa
     5341 ttcatctaaa tcaataaacg gtatttataa ccgtgcctta aaaaagatgg aaaaagaaaa
     5401 atataaagaa gctaaagatt tgtttattat tgtggaagca caagattcaa actacaaaga
     5461 attacaaaaa tatcttggaa tttgctatgt aaagcttgaa aactatgaaa aagccaaaga
     5521 ttattttgaa aaattaatta aaaccgcaag caaggatttt ctagttttac aaaatgcagc
     5581 gactgtgttt tttaatttaa aagattttca aagggcggaa gaattttata aaaaagcttt
     5641 agaaattaat aaggcaaatg cagactgttt tatgaatcta ggcatatctc aattctgtca
     5701 aaataaatat caagacgcaa ttgaatcgct taaagattac gaatttaaat ttggggccaa
     5761 cgttaaaaca cagttttaca tcaacagatg cgaagagtcc atttgcgatg aaaacgacaa
     5821 acaaaaaatg tctgagattc tgaatgaata cgtaagactt tcaaagctcc ccgaatttcc
     5881 aagagaattt gataacgtat tggctgtttt ctttgcaaaa atgggcaaaa tcgacttaat
     5941 gcttgaaagc tgcaataaaa taataagcta caatcccgaa gatactaatg catataaact
     6001 tttagggttg gttaacgttt ttatgaaaaa gcctgctcaa gcaaaaaatg atttatcaat
     6061 agctcttgac ctggatcctg aaaatgaaga agttaaagaa attttatctt ttatactttg
     6121 tcaggaagaa aaacatatgc aaagcaaaga atgcagggaa aaagtaagga attaataagt
     6181 aaatttatag aaggaagata atgtaaacaa tgtattttta agcacaaatt tttatatata
     6241 atattgttgg ttatgattgg agaaaatgta tgagtaaaaa agttttggtt attgatgatt
     6301 caccatttgt tttcaaagcg gtaagtaaag cccttgaagg aacagagtgg gaagttgtgg
     6361 gaaatgccct tgacggacaa ttgggtattg acaaatactt agaaatgtcc tgaccccgta
     6421 taattgtacc agttaaagag ttagtatttt gaaccgatag ttcaaaatac aagaaaggag
     6481 aaactggtat gcaaaaagga attaaggcaa aacctgagga aattatttct atcctcaggc
     6541 agattgaaat ccttacaaat caaggaaaca gcatccaaat gtcttgcaga gaagcaggaa
     6601 ttacagagca aacattctac cgctggagaa aacaatacgg cgggatgcag cttccgcaca
     6661 tcaaagaaat gaaaaaactt caagaagaaa atgtacggct taagaagatt gtagctgatt
     6721 taaccttaga gaaactgata ttaaatgaag tagttcaggg aaagttctaa gccctcagcg
     6781 aaagaaacaa gctgttattc acctcattca aaaattgaac atatccgagc gtaaagcctg
     6841 cacagcactg aaaataaaca gaatgaccaa gcggtatatt ccgcaaaagc caaatgatga
     6901 aaagcttttg cgagaagata tcgctcggct tgtgtcaaaa tacggcaggt acggttacag
     6961 aagaataaca gcatgtcaag cccccaattt ttgtaccaag ttaagagtta gtatttctgc
     7021 gtttaataat ttagttacag gagcaggggg cgcatagttc aaagaactat gcgggcgaat
     7081 ttcgttgtac tcccgtctgt aattttctgc cataactttt gcctctttca atgtataaaa
     7141 cacttctccg ttcaataatt catctcttag tattccgtta aacctttcgt taaagccatt
     7201 ctcccaagga cttccaggag tgatgtaagc agtctttact cggatttgtc gcaaaaaagc
     7261 ctgtatattt tttgcgacaa attccgaacc gttatcactt ctgatatact ctgggactcc
     7321 ttttgtaata aataattccg acaacacctc gattaaatcg tttgatttta agctgtaatt
     7381 gacctctatt ctcaaacatt ttctcgtaaa ttcatccaat atcgtcaaca ttctgatttt
     7441 cttgccgttg tataatttat cttgcacgaa atcgtaactc caaacatggt ttttatgcaa
     7501 aggtctgagc ctgatgcaag agccgtcatt aaagtacagc cttccgcgct tcttttgttt
     7561 ttgcgggact cttagccctt ctttccgcca aattctttca actcttttgt gatttactcg
     7621 ccagccctca gccctgagta
//
LOCUS       DAOQAU010000067         7578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_11721_length_7578_cov_7.004519, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000067 DAOQAU010000000
VERSION     DAOQAU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11721_length_7578_cov_7.004519"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKI94_08020"
     CDS             complement(<1..461)
                     /locus_tag="PKI94_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNW26718.1"
                     /translation="MDYYEILGVSKNAEKDEIKAAFRKKAREYHPDVNKAHDAEQKFK
                     ELGKAYETLMDDDKRSLYDRYGEDGLKNAGFGSEGPFAGGFGDLNDIFNSFFGGFGFS
                     QGYSDPNAPQRGDDLRVDIELTFEEAVFGITKEIKIDHLEICGECKGTGAEP"
     gene            complement(575..1177)
                     /gene="grpE"
                     /locus_tag="PKI94_08025"
     CDS             complement(575..1177)
                     /gene="grpE"
                     /locus_tag="PKI94_08025"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNW26719.1"
                     /translation="MKHKHRKEHEENQADNPFAEETAEERFEDAKMRRSEGEQENSHA
                     STHQLLQASDKLQQDFDNLNNQYLRLAADFDNYRKRQAQERETLIKYGAEDAMKKLIE
                     VLDNFDRAKQSIDNTDDVNQVKESFYVLYNQMFDNLSKLGLEVIEAQGKEFDPNLHEA
                     VMQTPTNDHEENHVIMELQKGYKLGDKVLRPALVNVATGE"
     gene            1459..1749
                     /gene="groES"
                     /locus_tag="PKI94_08030"
     CDS             1459..1749
                     /gene="groES"
                     /locus_tag="PKI94_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNW26720.1"
                     /translation="MSKIKPLSDRIVIKVIEDNEQTSGGIFIPDNAKEKPQKGEIIAV
                     GPGKILDNGQREPMDVKIGEVILYAKYSGTDVKIENETYKILSVKDALGILE"
     gene            1848..>3478
                     /gene="groL"
                     /locus_tag="PKI94_08035"
     CDS             1848..>3478
                     /gene="groL"
                     /locus_tag="PKI94_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNW26721.1"
                     /translation="MAKKIVFQEDARKSLLKGIDAVADAVKVTLGPKGRNVILEKKFG
                     APQIVNDGVTIAKEIELKDGLENAGAQLLKEVSSKTNDVAGDGTTTASVLAQAIVKEG
                     LKNLTAGSNPMCMKRGIDKAVELSVEKIKSMSKSVSTKEETAQVATISAGNASEIGEL
                     IAEAMDKVGHDGVITVEESKSFGTNLKVVEGMQFDKGYISPYFVTDAERMESVLEDAY
                     VLCVNKKINLIADLVPVLEQVARDGRSLLLIAEDVEGEALATLVVNTMRKVLRAVAVK
                     APGFGDRRKDMLEDIAILTGGQLFTEELGIKLENVTTQMMGLAKRVTVTKDETTIVVG
                     DETKKAVEERIKLIKKQIDASDSDYDKEKLQERMAKLTGGVAVIEVGAASEVELKERK
                     LRIEDALNATRAANEEGIVPGGGVALVRVQQELESKLPNIKFSTDDEKIGFKILLSAL
                     DVPLKLIANNAGAKSDVVIDKVRAEKDAFGYDALKNEFTDMIKAGIVDPAKVTRSALE
                     NAASVASMLLTTEAAVVEIQEDKPAMPDMSAMAGMG"
     assembly_gap    3479..3578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3603..4613
                     /locus_tag="PKI94_08040"
     CDS             3603..4613
                     /locus_tag="PKI94_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNW26722.1"
                     /translation="MKKRKIFALFISLLLLGLIFYKIDWSKLLQTFKTFNLKNLWTIV
                     PVYILTLYLRGIRWKNLLLNNPKYCAYNLGTVFTVGSMLNIYLPARAGDIYRAYYLGD
                     KQEEKKMKIFGSIILERTLDGICVFSILFCAVLLYSKTPWMLNLSYLIGAIFVGSFII
                     FYLIFKFNKIDFICSKLILFCQKLPEFLSGGLEKFIQKINKHANSFVEGFEVLNSPKC
                     VFQAFVMSFLIWFIECYVAFLIINSFNLTLGFSAALFVISLISFSTMIPSTSVFLGPY
                     QYAYILALGLYGIDKSTALAVSTVHQGILMVILSAIGLFYLFKFNFSLKESQKLEEAT
                     RL"
     gene            complement(4600..4758)
                     /locus_tag="PKI94_08045"
     CDS             complement(4600..4758)
                     /locus_tag="PKI94_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26723.1"
                     /translation="MDRMFIKEVSEFTETDENNNIIRKSKLVISNDGVETEIILEGNG
                     KIKVTTAV"
     gene            5098..5862
                     /locus_tag="PKI94_08050"
     CDS             5098..5862
                     /locus_tag="PKI94_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26724.1"
                     /translation="MIRVEPTTSATPPNVPQTPVPAPAPAKPSFKGERPDTAEFESMR
                     AEYQNLSNETSGLLSKIGKAGFLVLTGLIGYKTSKWGLQIVEKQAVKMVNAEKTQKII
                     SSVSGFAKDKVATKSKSLATSVLNFGKEKVMPLIAKGFKSIKNLKFVNKISNIFDTVA
                     LKSMYVMDSIKKSRAVNNKVSSKIIKAVSNSLSFVKNKSAKVWDFSKGMTVKAKENMP
                     TGTQIKNTAYETIAVSTGFATAMEGVDIANAVDEAA"
     gene            5864..6301
                     /locus_tag="PKI94_08055"
     CDS             5864..6301
                     /locus_tag="PKI94_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26725.1"
                     /translation="MNINKVQSITFGNKKASKDSFSGDEIAQGAVGGGSAAIIANGKK
                     LTKILPKKAAQKADTVLNGAEVVLKKSNAFSRLSNRFSVTFKGAIESLGNYKLLKPVA
                     KVAKSKIVGRVGALVGGFAAVGALAVNLATTVNVSGKLFDTNA"
     gene            6670..>7578
                     /gene="folP"
                     /locus_tag="PKI94_08060"
     CDS             6670..>7578
                     /gene="folP"
                     /locus_tag="PKI94_08060"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNW26726.1"
                     /translation="MDEFILKEIKNANLEKELEKIGFDKSYLHAAVDKFQYKNIKIFN
                     LTLPQANILKQTAISVGADCALHRESITAKIDKTNCILGGSFSQLKKIAQKLKKQPFK
                     LPKLADLIEGNLKSKLTPIKLKEKVFDFTRPYLIGVVNITKDSFSDGGDFFELGKAKE
                     HILKLIKDGADIIELGAETTKPFSSPTPEDNQLEKLLPILEFIKEEKIQTPVSIDTRS
                     ANVAKKCLEAGADMINDVSGLEFDKNLAQIVANFDVPIVIQHSKGTPETMQQNPCYES
                     LMDEIFLSLKEKIDFAISKGIKRENII"
BASE COUNT         2525 a         1274 c         1395 g         2284 t
ORIGIN      
        1 ggctctgctc ctgttccttt acattctccg cagatttcaa gatggtcaat tttaatttct
       61 tttgtaattc caaaaaccgc ttcttcaaag gtcaattcaa tgtcaactct taaatcatcg
      121 cctctttgag gcgcattggg atcggaatat ccttgtgaaa atccaaaacc gccgaaaaag
      181 gagttaaata tgtcgtttaa atccccaaat ccgcctgcaa acggtccttc tgagccgaat
      241 cctgcgtttt taagcccgtc ttcgccgtat ctgtcataaa gcgagcgttt atcatcatcc
      301 attaatgttt cgtaggcttt tcccaattct ttaaattttt gttcggcatc gtgtgcttta
      361 tttacatcgg gatgatattc tcttgctttt ttcctgaatg ccgcctttat ttcatctttt
      421 tcagcgtttt tagatactcc cagtatttcg taatagtcca ttttatttta aattccttca
      481 ttatatattt tagtaaatgg taaatagtaa attgtaaata gaagtaagga gtgaaatata
      541 aaaatttttc tattcactat ttcctatttc ctatttactc cccggtcgct acattaacca
      601 gtgccggtct tagaacttta tctcccagtt tatacccttt ttgtaactcc attataacat
      661 ggttttcttc gtgatcgttt gtgggagttt gcataacagc ctcgtgaaga ttgggatcaa
      721 attccttgcc ttgcgcctct ataacttcaa gtcccaattt tgaaagattg tcaaacattt
      781 gattataaag cacataaaaa ctttctttaa cctgatttac atcatcggtg ttgtctatcg
      841 attgctttgc tctgtcaaaa ttgtccaaaa cctcgattaa ttttttcatc gcatcttctg
      901 caccgtattt tattaaagtt tcgcgctcct gtgcctgcct ttttctgtaa ttgtcaaaat
      961 ctgccgccaa tcttaaatat tgattattta agttgtcaaa atcctgctga agtttatcgg
     1021 atgcttgaag gagttgatgc gtggatgcgt ggctgttttc ttgttctcct tcgcttcttc
     1081 gcatcttcgc atcttcgaat ctttcttccg ccgtttcttc agcaaaagga ttgtcggctt
     1141 gattttcttc gtgttctttt ctgtgtttgt gtttcatttt aatttctccc tgccgagcgt
     1201 cattgcgagg agaggcaaag cctcgacgcg gcaatctaaa atataaactt ttttggattg
     1261 cttcagcttt tttcaaaagc ttcgcaatga caaactattt aatattatag tttaacaagg
     1321 aacataaaca agttaaattt attttttttt aataatttaa cttcaattat taatttttca
     1381 ttaaaaatgt attttataga aaaatttcgg ggtataaata gaattgacag gttgtattac
     1441 aaaagaagga gggaaactat gtcaaagatt aaaccgcttt cagacagaat agtcattaaa
     1501 gttattgaag ataacgagca gacatcagga ggaattttta ttcccgataa cgccaaagaa
     1561 aaaccgcaaa aaggcgaaat tattgccgta ggcccgggta aaatacttga taacggacaa
     1621 agagagccta tggatgtaaa aatcggggag gttattttat acgcaaaata ttccggaacc
     1681 gatgtaaaaa tagaaaacga aacttacaaa attctttcgg tgaaagatgc tttgggaatt
     1741 ttagagtagt aaatagcaaa aagtaaatag taaaaaggat gaaacgaaaa atttttctat
     1801 tcactatttc ctatttacca tttacttaat taaaaggaga aataaaaatg gcaaagaaaa
     1861 tcgtatttca agaagacgcg cgcaaaagcc tgttaaaagg tattgatgcg gttgcagatg
     1921 ctgtgaaagt aacattggga ccaaaaggcc gcaatgtaat attagaaaaa aaattcggtg
     1981 cacctcaaat tgttaatgac ggtgttacca ttgcaaaaga aatcgaactt aaagacgggc
     2041 ttgaaaatgc aggtgcccaa cttttaaaag aagtttcttc aaaaacaaat gatgttgcag
     2101 gggacggaac aacaacagcg agtgttttgg ctcaagctat cgttaaagaa ggcttgaaaa
     2161 atcttaccgc aggatcaaat cctatgtgca tgaaacgcgg aattgacaaa gcggttgaac
     2221 tttctgtaga aaaaattaaa tcaatgtcaa aatctgtaag taccaaagaa gaaactgcac
     2281 aagttgctac aatttctgca ggtaacgcaa gtgaaatagg tgaacttatc gcagaagcaa
     2341 tggataaagt cggacacgat ggcgttatca cggtagaaga atctaaatct ttcggtacaa
     2401 atttaaaagt cgtagaaggt atgcagtttg acaaaggcta catttcgcct tactttgtaa
     2461 ccgatgcaga gcgcatggaa tctgttttgg aagatgctta cgttctttgc gttaacaaga
     2521 aaattaatct tatcgcagac cttgttcccg tgttggagca ggttgcaaga gacggacgct
     2581 cactgctttt aatcgcagaa gacgttgaag gcgaagcact tgcaaccctt gttgtaaaca
     2641 caatgagaaa agttttaaga gctgttgcgg ttaaagcccc gggatttggc gacagaagaa
     2701 aagacatgct tgaagatatt gcaatcttaa caggcggtca attattcaca gaagaactcg
     2761 gaattaaact tgaaaacgtt acaactcaaa tgatgggact tgcaaaaaga gttaccgtta
     2821 caaaagacga aacaactata gtagtaggcg atgaaacaaa aaaagcggtt gaagaacgta
     2881 ttaaattaat caaaaaacaa atagatgctt cagattccga ttacgacaaa gaaaaacttc
     2941 aagaacgtat ggcaaaatta acaggcggcg ttgcggttat cgaagtgggc gcggcttctg
     3001 aagttgaatt aaaagaaaga aaattaagaa tagaagacgc actcaatgcg acacgcgcgg
     3061 ctaacgaaga aggcatagtc ccaggcggcg gagttgcact tgtgagagtt caacaagaac
     3121 ttgaaagcaa attgccaaat attaaatttt caacagacga tgaaaaaatc ggattcaaaa
     3181 tcctgttatc agcacttgat gttcctttaa aactgatagc aaataacgca ggcgcaaaaa
     3241 gcgatgttgt aatagacaaa gtgagagccg aaaaagacgc tttcggttac gatgctttga
     3301 aaaacgaatt taccgacatg attaaagcag gaatcgttga ccctgcaaag gttacacgct
     3361 ctgcgttgga aaatgcggca tcggttgctt caatgctgtt gacaacagaa gcggcggttg
     3421 tggaaatcca agaagacaaa ccggcaatgc ctgatatgtc tgctatggca ggcatgggnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ccttttatgt taaatttaaa
     3601 atatgaaaaa gaggaagatt tttgcattat ttataagttt gttgcttttg gggctgattt
     3661 tttacaaaat cgactggtca aaactgttgc aaacattcaa aacttttaat ttaaaaaatt
     3721 tatggactat tgttccggtt tatattttaa ccctttattt aaggggaatt cgctggaaaa
     3781 atctgctttt aaataatccc aaatattgcg cttacaattt aggaacagtt tttacagtcg
     3841 gaagcatgct caatatttat cttcccgcac gcgcgggaga tatttatcgg gcttattatc
     3901 tgggagataa acaggaagaa aagaagatga aaattttcgg ctcaataatt ttggagagga
     3961 cattagacgg aatttgtgtt ttttcaattc tgttttgtgc tgtactttta tattcaaaaa
     4021 ccccttggat gcttaattta tcttatctta taggggcgat atttgtggga agttttatta
     4081 ttttttatct gatttttaaa ttcaataaaa ttgattttat atgttcaaaa ttgattttat
     4141 tctgccaaaa actacccgaa ttcttatccg gaggtcttga aaaatttatc caaaaaataa
     4201 ataaacacgc aaactccttt gtagaaggat ttgaagtctt gaatagtccc aaatgtgttt
     4261 ttcaagcttt tgtaatgagt tttttaattt ggtttataga atgttatgta gcttttttaa
     4321 taataaacag ctttaattta acacttgggt tttcggcggc actttttgta ataagcttaa
     4381 tctctttttc aacaatgatt ccttcaactt ctgtattttt aggaccttat caatatgcct
     4441 atattttggc attggggctc tacggcatag acaagtcaac agcacttgcc gtttcaacag
     4501 tacatcaggg aatcctaatg gtaatactct cggccatagg attgttttat ttgtttaaat
     4561 tcaacttttc actaaaagaa agccaaaaat tggaagaagc tacacggctg tagtaacttt
     4621 gattttgccg ttgccttcaa gaataatttc tgtttcaaca ccgtcatttg aaataacaag
     4681 cttcgattta cggattatat tgttattttc gtctgtttct gtaaattcgc tgacttcttt
     4741 tataaacatt ctatccatga tttttacctt tcgttgttgt ctgcacaaga cttaaggggg
     4801 gctattactt atgataacgc attttttgta aatttcaacc cctttggagt tacacatttt
     4861 gttaaaaccg aaaagaaaaa atttttgcct gaaattttca aaaagggtaa ttatttttaa
     4921 aaattcttaa catttttatg gcatgttaaa gtgtttacat ttgtgttaaa atatgcaata
     4981 atatgccatg agaaattata gtgtaaataa ttgtaacaaa agtaatttca tgtagcaaaa
     5041 aaatttttta gggtttttaa attaaggtaa gaaatattaa tttatggagt aaaaaatatg
     5101 attagagtag aacccacaac aagtgcaacc cctccaaatg tgcctcaaac acctgtgccg
     5161 gcacctgcac ctgcaaagcc aagctttaaa ggcgagcgtc ctgacaccgc agaatttgaa
     5221 agcatgcgtg cagaatatca aaatttaagc aacgaaacat cagggcttct ttctaaaata
     5281 ggtaaagccg gtttcttggt tttaacaggt ttaatcggtt ataaaacctc aaaatggggc
     5341 ttacagatag ttgaaaaaca agcagtaaaa atggttaacg ccgaaaaaac tcaaaaaatt
     5401 atatcaagtg tttccggttt tgccaaagat aaagttgcaa caaaatcaaa aagtcttgca
     5461 actagtgttt taaacttcgg taaagaaaaa gttatgcctt taattgctaa aggttttaaa
     5521 tccattaaaa acttaaagtt tgttaataaa atttcaaata tttttgacac tgtagcctta
     5581 aaaagcatgt acgttatgga ttcaattaaa aaatcccgtg ctgttaataa caaagtttct
     5641 tcaaaaatta taaaagctgt ttcaaacagt ctttcttttg taaaaaacaa atccgcaaaa
     5701 gtttgggact tttcaaaagg aatgacagtt aaagcaaaag aaaatatgcc tacaggcact
     5761 cagattaaaa ataccgcata tgaaaccatt gctgtttcaa caggttttgc aaccgctatg
     5821 gaaggtgtag atattgcaaa tgcagtggac gaggcagctt aatatgaaca tcaacaaagt
     5881 acaatcaata acatttggaa ataaaaaagc ttcaaaagat agtttttcgg gtgatgaaat
     5941 cgcacaaggt gctgtgggcg gaggaagtgc cgcaataata gctaacggca aaaaattgac
     6001 taaaatcctg cctaaaaaag ccgctcaaaa agccgatact gttttaaacg gagccgaggt
     6061 tgtgttgaaa aaaagtaacg ctttttcaag gctttcaaat agatttagtg taacatttaa
     6121 aggtgctatt gaatctttag ggaattataa actcttaaag cctgtagcaa aagttgctaa
     6181 aagcaaaata gtaggaagag tcggcgcact tgtcggcggg tttgctgcag taggtgcttt
     6241 ggcggtaaat cttgcaacaa ctgtaaatgt tagcggaaaa ctttttgata ctaacgctta
     6301 gttaatttaa tataaaaact gagagggcgg acttgatgac tatggcaagt tcccctcttt
     6361 tttatgtatt gagccatata ttatttatgt tagttccctt cccctcgccc cttgagggag
     6421 agggaaattt ttctgtgcga ggaacgaaca cttgaaaaat gggtgagggg taaaaagcgc
     6481 acatcttccg catggacccc tcacctgctt ttctaatact cgcaggtttc tgctcgcatt
     6541 gaaaagcatc ctctccctca aggggcgagg gggtcaaaat aaaaaaggaa aagaaaccga
     6601 accaaaagaa aaaaataaaa aatactgact ttaaatcagc tattcaatga gcatgttata
     6661 attaactcta tggacgaatt tattttaaaa gaaattaaaa atgcgaattt agaaaaagaa
     6721 cttgaaaaaa taggctttga caagtcttat ttgcacgctg ccgttgacaa atttcaatat
     6781 aaaaacataa aaatatttaa cttaaccctt ccgcaagcaa atattttaaa acaaaccgca
     6841 atttctgtag gtgcggactg tgcacttcac agagaatcaa taaccgcaaa aatcgacaaa
     6901 acaaactgca ttttgggcgg aagcttttcc cagcttaaaa aaatagcaca aaaactcaaa
     6961 aaacaaccgt ttaagcttcc taagcttgca gatttaatcg aaggtaacct taaatcaaaa
     7021 ttaaccccga taaagcttaa agaaaaggtt tttgacttca caagaccata tcttataggc
     7081 gtggtaaata ttacaaaaga ttctttttca gacggcggcg atttttttga acttggcaag
     7141 gcaaaagagc atattttgaa attaatcaaa gacggtgcag atataataga gcttggcgca
     7201 gaaaccacaa agcctttttc atcgccgaca cccgaagaca atcagcttga aaagcttttg
     7261 cccattttag aatttataaa agaagaaaaa attcaaacac ccgtaagcat tgatacaaga
     7321 agcgcaaatg tcgcaaaaaa gtgccttgaa gcaggtgccg atatgattaa cgatgtatca
     7381 gggcttgaat ttgataaaaa tttggcgcaa atagtagcaa attttgatgt tccgattgta
     7441 attcagcatt caaaaggcac gcctgaaact atgcagcaaa acccttgtta tgagagttta
     7501 atggatgaaa tatttttgtc tttaaaagaa aaaattgatt ttgcaatctc aaaaggaata
     7561 aagcgagaaa atataata
//
LOCUS       DAOQAU010000068         7131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_12807_length_7131_cov_6.369276, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000068 DAOQAU010000000
VERSION     DAOQAU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12807_length_7131_cov_6.369276"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="rpmF"
                     /locus_tag="PKI94_08065"
     CDS             <1..346
                     /gene="rpmF"
                     /locus_tag="PKI94_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNW26727.1"
                     /translation="KMAVPKKRTGHSAQGHRRANWKATKPEITKCSKCGEPVLTHTVC
                     AACGYYKGKAVSIKSSDYSESKLEPKKSVKKAVKKAEPKPKKAEEKAEEIIEEKSVEV
                     VEQIKEEEPKGE"
     gene            412..1464
                     /gene="plsX"
                     /locus_tag="PKI94_08070"
     CDS             412..1464
                     /gene="plsX"
                     /locus_tag="PKI94_08070"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNW26728.1"
                     /translation="MTRIAIDAMGGDHAPREIVAGAIWAASDYGVAIELVGKQDQIEY
                     EIDKISREGIVSTAGGFKNKKIRVDVSKLDIKITPATEVIEMGEAPGQAIRKKKKSSI
                     VLAVDAVATGSSEAIVAAGSTGAAMASSLFGLGRIPGIDRPAIAITLPTMKDPIVVID
                     AGANSNCSPEMLFQFAVMGTTFSKNVLNIENPRVGVLNIGEEAGKGNELAQATYKLLE
                     ENQDKINFIGNIEGREIFSGVCNVVVCDGFVGNVALKITEGTSSMLFRMLKQEFKTDP
                     LARIAGLIAKPFMKRIYKKINYEEFGGALLLGVKGITVISHGSSKAYAIKNAVRVAKE
                     AVETGVNKKISEFLGE"
     gene            1494..2501
                     /locus_tag="PKI94_08075"
     CDS             1494..2501
                     /locus_tag="PKI94_08075"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNW26729.1"
                     /translation="MTRQLIPLKIAGVGYCVPETVITNDDLTKLYDTSDEWIYTRTGI
                     KERRVVSGEENAIDLGYEAAKNALKKANIKPEEIDMIIAASSAPADLYPAMACRIQAK
                     LGVKEIPAFDVTAACSGLIYTMEMARAFIGAGIHKKILIVATDNNSRFCDWTDRSVSI
                     LFGDGAGAMVVTEAEDGINDLLAIDIKADGSIGEYITLPFTGQNCPLVTPCEEKPSHV
                     KMNGREVYKFAVRIVPEYIDKCLEMANMKAEDVDYFIPHQANQRIIEAVQQRLGYSDE
                     KVISNIKYYGNTSAASVPIALAEGVEHGKIKLPCTAILCGFGAGMTWGVAIVRLRKGI
                     C"
     gene            2593..3525
                     /gene="fabD"
                     /locus_tag="PKI94_08080"
     CDS             2593..3525
                     /gene="fabD"
                     /locus_tag="PKI94_08080"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNW26730.1"
                     /translation="MTKKIAFIFPGQGSQSVGMGKDLYDNFEQARHVFDKANEVLGKD
                     IKKICFEGPDEDLKQTINTQPAILTTSIAALEALKSKLNIKPELVAGHSLGEYGALYE
                     AGVFDLETAFKLIQKRAEFMNGVKGGTMAAVLGANEDVLIDCLQKASDEGYVAVANYN
                     SPDQIVITGSVEGVKKASELLMLAGAKRVIPLAVSGGFHSELVKNAGEKFSVYLDDYV
                     LNQAKIPVLTNVDAEITTNAEDFKLKMPKQMYSSVYWTQTIQKMIKEGVDTFIEIGPG
                     KVLAGLNKKIAPEVGTYNVYDKVSLDTCVEELNK"
     gene            3539..4276
                     /gene="fabG"
                     /locus_tag="PKI94_08085"
     CDS             3539..4276
                     /gene="fabG"
                     /locus_tag="PKI94_08085"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNW26731.1"
                     /translation="MSEKKVALITGAARGIGKACAIELAKNGYDIVVNDIALGTNEET
                     ANETAKELRAFGVDTMVVMFDVTDKEAIEKGVEKIVEKFGRIDVLVNNAGITRDNLLV
                     RMTQENWLAVINTNLNSAFYVSTPVVKVMMKQRFGSIINMASIAGVYGNAGQPNYSAA
                     KAGLIGLTRSMAKELGSRNIRVNAVAPGFINTAMTRELKLDGIEEHIPLRRLGEVEDI
                     AAAVKFLAMDATYITGQVLQVDGGLVI"
     gene            4396..4626
                     /locus_tag="PKI94_08090"
     CDS             4396..4626
                     /locus_tag="PKI94_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNW26732.1"
                     /translation="MSTLEKVKKVVVEQLSVDEALVTPEASFTGDLGADSLDTVELVM
                     AFEEEFGCEIPDEEAEKIQTVQDAVNYIDSHS"
     gene            4743..5993
                     /gene="fabF"
                     /locus_tag="PKI94_08095"
     CDS             4743..5993
                     /gene="fabF"
                     /locus_tag="PKI94_08095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNW26733.1"
                     /translation="MDKKRVVITGIGMVSPFGVGTDIFWKSLLEGKSAIRTIQGMDLE
                     KHHVKIAGQVLDFNPEDYMDPKEAKKMDRFTQFALVASDEAIKDAGLDKIIDFDPYRY
                     GVMVSSAAGGFHTFEKNHIAMIERGPTKCSPFTIPMLIVDMSSGRVSMKYGYKGVNKA
                     VVSACATGSHSIGDAFRSIQYGDADVILAGGCEATICNLGIGAFTSARTLSRRNDEPQ
                     RASRPYDKDRDGFVMAEGAGVLIMEEYEHAKARGAKIYAEIVGYGQTADAYDIVAPDP
                     EGTGAQKAMEFALKDANLKPEEIQYINSHGTSTGLGDAAESHAIAKVFGDLDVNKNLL
                     VSSTKSMHGHMLGATGAAESIVCIKAINEGVVPPTINLDNQDEEVANLDYVPHKTRKK
                     EVSAALTNSFGFGGHNASLIFKKI"
     gene            6536..7105
                     /locus_tag="PKI94_08100"
     CDS             6536..7105
                     /locus_tag="PKI94_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26734.1"
                     /translation="MISPIGFNSIAFSSKIDKSRLNRNVSFTSSDIGSSDFSSFLESK
                     TYDYACVDKSRDFFVGAWPMSDNDMIFLANKKATVIDFDYDKIMGTKKFAKNLGLKYL
                     GLDDIADPKNIKACYRGIVNKMKEAKGPVLLHSFRAKDEPFYMGALYMIKEKGISVKE
                     AAEIIEHKILIQPRFDLIEALEKMLKQLL"
BASE COUNT         2480 a         1077 c         1576 g         1998 t
ORIGIN      
        1 gaaaatggca gtaccaaaga aaagaacggg gcattcggct caaggacata gaagagcaaa
       61 ctggaaagcg acaaaacccg aaataacaaa atgttcaaaa tgcggtgaac ctgttttgac
      121 acacacggtt tgtgcggctt gcggatatta taaaggcaaa gcggtaagca ttaagtcatc
      181 ggattattca gaatcaaaac tcgagcctaa aaaatctgta aaaaaagcag ttaaaaaagc
      241 agaaccgaaa cctaaaaaag ctgaagaaaa agctgaagaa ataattgaag aaaaatcagt
      301 tgaggttgtt gagcaaataa aagaggaaga acctaagggc gaataggtaa atagtgaata
      361 aattctattt actatttaca atttactatt cacttaaaaa agaggggaaa tatgacaaga
      421 atagctatag atgcaatggg cggcgaccat gcacctcgtg aaatagtagc aggtgctatt
      481 tgggctgctt cagattatgg tgtcgcaata gaattggtcg gaaagcaaga ccagattgaa
      541 tatgaaattg ataaaataag cagagaagga attgtttcaa ctgctggcgg atttaaaaat
      601 aaaaaaataa gggttgatgt cagtaaactt gatatcaaaa ttacaccggc tacagaagtc
      661 attgaaatgg gcgaagcacc cggtcaggca ataagaaaga agaaaaaatc ttcaatagtt
      721 ttggcggttg atgctgtagc tacaggaagt tcagaggcaa tagtggcagc aggttcaaca
      781 ggtgctgcta tggcttcaag tttgttcgga ctgggaagaa ttcccggaat tgacagaccg
      841 gctattgcaa taactcttcc tacaatgaaa gatcctattg ttgttataga tgcaggtgca
      901 aacagtaact gctcgcctga aatgttattt cagtttgcgg ttatgggaac aactttttca
      961 aaaaacgtac taaatattga aaatcccaga gtaggcgttt tgaacatcgg tgaagaggca
     1021 ggaaaaggaa acgaacttgc tcaggcaact tacaaattat tggaagaaaa tcaagataaa
     1081 attaacttta taggtaacat tgaaggcaga gaaattttct caggtgtttg caatgtagtg
     1141 gtttgcgacg gttttgtcgg aaatgtggca ttaaaaatta ccgaaggaac atcttcaatg
     1201 cttttcagaa tgctcaaaca ggaatttaag acagatccgc ttgcaagaat cgcagggttg
     1261 attgcaaaac cttttatgaa aaggatttac aaaaaaatca attacgaaga attcggcgga
     1321 gctttgctct tgggggttaa aggaattacc gtaatctcac acggaagttc taaagcctat
     1381 gcgattaaaa acgcggttag agttgcaaaa gaagctgttg aaacaggcgt aaataaaaaa
     1441 atatcagaat tcttgggcga atagccttta tattaaaata cgagaggtgg aaaatgacaa
     1501 gacaattaat tccgttgaaa attgccggag taggttattg tgtgcctgaa acggttataa
     1561 caaatgatga tttgacaaaa ttatatgata catcggacga gtggatttat acaagaaccg
     1621 gtataaaaga acgtcgtgtg gtatccggtg aagaaaatgc aatagatttg ggctatgagg
     1681 cggcaaaaaa cgctcttaag aaggcaaaca ttaagcccga agaaattgat atgattattg
     1741 cagcatcatc tgcaccggct gatttatacc ctgcaatggc atgcagaatc caagccaaat
     1801 taggagttaa agaaattcct gcatttgatg ttacggcagc atgttcaggg cttatttata
     1861 ccatggaaat ggcaagagcc tttataggag caggcattca taagaaaatt ctaattgtag
     1921 caacagataa taattcaaga ttttgtgatt ggacagacag aagtgtttca attctttttg
     1981 gtgacggtgc aggcgcaatg gttgtaacag aagccgaaga cggtataaac gatcttctgg
     2041 caatagatat taaagcagac ggttcaatcg gagaatatat aacacttcct tttacagggc
     2101 agaattgtcc tttggttacg ccttgcgagg aaaaaccttc tcatgtaaaa atgaatggac
     2161 gcgaagtgta taaatttgct gttagaattg ttccggaata tattgacaaa tgtttggaaa
     2221 tggcaaatat gaaagcagaa gatgttgatt actttattcc tcatcaggca aatcaaagaa
     2281 ttatagaagc agttcaacaa agactaggct acagtgacga aaaagttatt tctaatatca
     2341 aatactacgg taacacttca gccgcctctg ttccgattgc gcttgcagaa ggcgttgaac
     2401 acgggaagat aaagcttcca tgtacggcaa ttctttgcgg attcggtgca ggcatgacct
     2461 ggggtgttgc tatcgtaaga ctcagaaaag gaatttgtta gttgaaaggc ttaatgggtg
     2521 aatagttaaa tttaactttt aagccattca cctgttcatc tattcagcta aaagatataa
     2581 aaggaatgta tcatgacaaa aaagatagcg ttcatattcc cagggcaagg ctcgcaatct
     2641 gtcgggatgg gaaaagattt atacgataat tttgaacagg caaggcatgt ttttgacaag
     2701 gcaaatgaag ttttgggcaa agacattaaa aaaatatgtt ttgaaggacc ggatgaagat
     2761 ttaaaacaaa caataaacac ccagcctgca attttaacta cttcaattgc tgcattggaa
     2821 gctttgaaat caaaattaaa tatcaagccg gagcttgttg cgggacacag tttgggcgaa
     2881 tatggggcac tttatgaggc aggggttttt gaccttgaaa cagcttttaa acttattcaa
     2941 aaaagagccg agtttatgaa tggagtaaaa ggcgggacaa tggctgcggt tttgggtgca
     3001 aatgaagatg ttttgattga ctgtcttcaa aaagcctctg atgaaggcta tgtggcggtt
     3061 gcaaattaca attcgcccga tcagatagta ataacaggtt ctgtcgaagg ggttaaaaaa
     3121 gcatccgaac ttttaatgct ggcaggtgct aaaagggtta ttcctcttgc ggtttcagga
     3181 ggttttcatt ccgagcttgt taaaaatgca ggcgaaaaat tttcggttta cctggatgat
     3241 tatgtattaa accaagcaaa aatacctgtt ttaaccaatg ttgatgctga gattaccaca
     3301 aatgccgaag attttaaatt aaaaatgcca aaacaaatgt attcaagcgt gtattggact
     3361 caaaccattc aaaaaatgat aaaagaaggg gttgatacat ttatagaaat aggaccggga
     3421 aaagttttgg cagggcttaa taaaaaaata gcacctgaag ttggaactta caatgtttat
     3481 gataaagtat cactagatac ttgcgtcgaa gagttaaata aataaaggag aaaaagagat
     3541 gtcagaaaaa aaagtggcac ttataaccgg tgcggcaaga gggataggaa aagcttgtgc
     3601 aatcgagctt gcaaaaaacg gttatgatat tgtggtaaac gacattgcgt taggcacaaa
     3661 cgaagagacg gcaaatgaaa cagcaaaaga attaagagct tttggcgttg atacaatggt
     3721 tgttatgttt gatgttacag acaaagaagc aattgaaaaa ggggtagaaa aaatcgttga
     3781 aaaattcgga aggattgatg ttcttgtcaa taatgcagga attactcgcg acaatttgct
     3841 tgtgagaatg actcaggaaa attggcttgc tgttattaac acaaatttaa acagtgcgtt
     3901 ttatgtttca acacctgttg ttaaagttat gatgaaacag cgttttggtt ctatcataaa
     3961 catggcaagt atagcaggag tttacggaaa tgccggtcaa cctaattatt cagctgcaaa
     4021 agcaggttta atagggctta caaggtcaat ggcaaaagaa ttgggttcaa gaaacatccg
     4081 tgtgaacgct gttgcgcctg gatttattaa taccgcaatg accagagagc ttaaattgga
     4141 cggaatagaa gaacatattc cgctcagaag gcttggtgaa gttgaagata tagccgccgc
     4201 agttaagttt ttggctatgg acgctactta catcacgggt caagttctac aagttgacgg
     4261 tggattggtc atttgaccaa aaattcaaag aacttaacaa taagcatttt tttatgttaa
     4321 tttcttgcaa aaaattcttt aatcggtata atttttatgg atagttaaga gaaacacaaa
     4381 gaaaagggaa agaaaatgag cacattagaa aaagttaaaa aagtagtagt tgaacagttg
     4441 agcgttgatg aagcactcgt aactcctgaa gcaagcttta cgggcgattt aggtgcagat
     4501 tcattggata ctgttgagtt ggtaatggct tttgaagaag agttcggatg cgaaattcct
     4561 gacgaagaag ctgaaaaaat tcaaacagtt caagatgctg taaactatat cgattctcat
     4621 tcgtaatcga ttttaaggtt aagctgtaac aagagagggg ctttaagttc tcttaattaa
     4681 gggagaaatc ccctccttgt tatatgttta tttttatcaa actagtaaaa aggtaaaaac
     4741 ttatggataa aaaaagagta gtaataacag gtatcgggat ggtgtcacct tttggtgtgg
     4801 gtactgatat tttttggaaa agccttttag aaggcaaaag tgctataaga acaattcagg
     4861 gcatggattt agaaaagcat catgtaaaga ttgcaggaca agtccttgat tttaatcccg
     4921 aagattacat ggatcccaag gaagcaaaaa aaatggatcg ctttacccaa tttgcacttg
     4981 ttgcctcaga tgaagcaatc aaagatgcag ggttggataa gattatagac tttgatcctt
     5041 acagatacgg agttatggta agttctgcag caggcgggtt tcataccttt gaaaaaaacc
     5101 acatagctat gattgaaaga ggacctacaa aatgttcgcc ttttacaatc cctatgttaa
     5161 ttgttgatat gagctcaggc agagtgtcaa tgaaatacgg ctataaaggt gtaaacaaag
     5221 ccgttgtatc agcttgtgca acaggctcac attctatagg agacgcattc agatctatac
     5281 aatacggaga tgctgatgta atacttgcag gcggttgtga agctacaatt tgtaatcttg
     5341 gaataggtgc ttttacaagt gcaagaacac tttcaagaag aaacgacgag cctcaaaggg
     5401 cttcaagacc ttatgacaaa gacagagacg gttttgttat ggcagaagga gccggtgttt
     5461 tgataatgga agagtatgag catgccaaag caagaggtgc taaaatttat gcagaaatag
     5521 taggctatgg tcaaacagcc gatgcttatg atattgtagc tcctgatcct gaaggtacag
     5581 gtgcgcaaaa agcaatggaa tttgcgctta aagatgcaaa tcttaaaccc gaagaaatac
     5641 aatatataaa ttctcatgga acaagtacag gattgggcga tgccgctgaa tcccatgcca
     5701 ttgcaaaagt ctttggtgac cttgatgtaa ataaaaatct tcttgtaagt tcaacaaaaa
     5761 gtatgcacgg tcatatgctc ggagcaacag gtgcggcaga gagcattgtt tgtataaaag
     5821 caataaatga aggagttgtg ccacctacca ttaatcttga taatcaagat gaagaagttg
     5881 ctaatcttga ttatgttcct cataaaacaa gaaaaaaaga agttagtgca gctttgacaa
     5941 attcattcgg gtttggcggg cataatgctt ctttgatatt taagaagata taattaagaa
     6001 ttaagataag ataaagcggc ggattttcgt gaaacgaaaa tccgccgctt tatctttcga
     6061 ttttaaggaa aaaaagaact tttttcctta aaatctgatg tccggtttca aaccttaccg
     6121 ccggctaacc cgcagtcggc aaggttttca cacttctaag gtcgcagatg aaaaagcctc
     6181 gattttccca aaaatcgagg ctttttcatc tgctctttca gctatttacc aacggggcta
     6241 gattttatta attctttttg tcaaagaatc gataaattct ttgatttttt taagtaataa
     6301 ttttaattcg ccactgatac tgcaagcacc tggccaagta taactgggat acattttatt
     6361 ttctccttaa atttcgatac tttatcctat ggcagtaatt tcgggttttt gaggtaaaac
     6421 tttagtgttt taatataaaa tgatacactt ctttacaata aggtgggttt ttagcaatta
     6481 ttttttttag ggtcattatt aattacataa tagcaaaact ctttttaagg taaaaatgat
     6541 ttcacctata ggttttaaca gtatagcatt tagttccaaa atcgataaaa gcaggcttaa
     6601 tcgaaatgtc agctttacgt ccagcgatat tggctcaagt gattttagtt cgtttttaga
     6661 atcaaaaacg tatgattatg cctgtgtaga taaaagcagg gatttttttg taggggcatg
     6721 gccaatgagc gataacgaca tgatttttct tgcaaataaa aaggctacgg ttatcgattt
     6781 tgattatgat aaaataatgg gaacaaaaaa atttgcaaaa aatttggggc tcaagtattt
     6841 aggtttggac gatatagcag atcctaaaaa tatcaaagct tgttatcgtg gaattgttaa
     6901 taaaatgaaa gaagcaaaag gtccggtttt attgcattct tttagggcaa aggacgagcc
     6961 tttttatatg ggagcccttt atatgattaa agaaaaaggg atttccgtta aagaggcagc
     7021 agaaataatt gaacataaaa ttttaattca gcctcgtttt gatttaatcg aggcgttgga
     7081 aaaaatgctg aagcagttgt tgtagaattt taatataaat atttttattt a
//
LOCUS       DAOQAU010000069         6869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_13468_length_6869_cov_7.356619, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000069 DAOQAU010000000
VERSION     DAOQAU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_6869_cov_7.356619"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1400)
                     /locus_tag="PKI94_08105"
     CDS             complement(18..1400)
                     /locus_tag="PKI94_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26735.1"
                     /translation="MIDFQNATQVQNIQTPPAIQQQVEVKTPDTTNLLTQYKNDVPAW
                     AYRGAAFMENKNLNVQVANLNLEQYKEPAKIAVEDISSRAGKPGQFLNWIDVLPKTEL
                     QSLDSIYDTANKAKQGGFTDLAVIGIGGSRHTNEAMMKMLGKDPHVHFYSGIDPDSFK
                     RFADSLDLDKTKFLIVSKSGGTLETTVGYESARKLMQEHLKKEDVSDRFIAMTDASAE
                     KSKLRQAVDRGEFSASGLVHDDVGGRFSIFDDATIFTMAYAGVSKEDSKKMLEASLTA
                     QKEFLNPDINKNEALKLAAFNADAKSNGRNKHFVEYFGDAFAGATLWEKQMKNESLKS
                     KISTDTNIGPGYLHYNAESDLDPNNKDSFYTFIYVKPQDKKTEALLNGAMTAYSAQHP
                     VSKIEFKDLSPETIARFIELKHFETLYTGNMLRQKAGEKTPEGSAMAEVLQPNVEIYK
                     KEVKKVLEPK"
     gene            complement(1899..1982)
                     /locus_tag="PKI94_08110"
     tRNA            complement(1899..1982)
                     /locus_tag="PKI94_08110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1947..1949),aa:Leu,seq:caa)
     gene            2134..2922
                     /locus_tag="PKI94_08115"
     CDS             2134..2922
                     /locus_tag="PKI94_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNW26736.1"
                     /translation="MFENLILLTVFFMLFASLPAFSESMQNKVVVKGADGINRIIDSK
                     TNKPIGKAKIVVPQSNYQTYTDEQGRFQLGAKVDGQTVMSVEKEGYKPFSVTLDKNNN
                     AQAIVLGIEKSTPRDIVIDTEMFHLGDNNFSDASANAREFRVNSVGPFYSKKFKLSSV
                     PQGSSTFLVIGSIIGIDTKLAKSMGQNKITNSYASPPEIYFNGNKIAEIQLNGDGQKI
                     KLPSSLIRQNMMNEVTIKTGRNLMQTAYVDYDDIEFMNLIIENK"
     gene            complement(2925..6368)
                     /locus_tag="PKI94_08120"
     CDS             complement(2925..6368)
                     /locus_tag="PKI94_08120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNW26737.1"
                     /translation="MQYVPLHLHSEYSLLDGAIKIKELCEFAKQNDMPAVAVTDHGAM
                     YGAIDLYKTAKDCGVKPLIGCEFYVYQGDISEKNQSKTHLNHLVLIAKDKQGYANLVK
                     LVSIAHCEGFYYKPRVNFELIKQYHQGLICLSSCVQGELADLILQKDIKAAKERALKY
                     KELFGEDYYIELQDHGLDKQKRSNPDLINLAKELGIKIVITNDSHYLKKEDADWHDTL
                     LCLQTNALKTDENRFHFPNNEFYVKTVSELRDSFKWLDSETFEKAIKNTVEIAEKCHL
                     IIEMGGSLLPKYEVPKNHTTESYLDFKVREGLEKRYKEITPEIEKRVKYELEIIEQMG
                     FSAYFLITWDFINFAKQNGIPVGPGRGSAAGSIVAYALEITDLDPIRHNLLFERFLNP
                     ERISMPDVDIDFCIERRSEVIDYVSKKYGEDHVCQIVTFGTLAAKAALKGVARVFNIP
                     FAESNKLSALIPSTPGAKIDDALKEGMELKILYDNDPEIKKLVDMAKGLEGVKNNIGM
                     HAAGVIISHKPLNEIVPVQPSKDGSIITQYPMTDLEKLGLLKMDFLGLRNLTIISKTI
                     KMIKTRHGLDIDINNIPLDDKKTYEMLTRGETDGVFQLESTGMKRLVRDLKPSVFEDL
                     GALVALFRPGPLESGMVEDFVQRKHGRQAITYDHPLLEPILKDTYGTIVYQEQIMQIF
                     QTLAGYSLGQADMVRRMMGKKKVEEMAKQKEKFIEGAGKNGMMPKEATELFEQIEKFA
                     EYCFNRSHSAAYAFVAYQTAYLKTHYTVEYLSALLSSVANDQDKTQAYIEEALKYGIK
                     VLPPDINKSFAEFTPDEKNIRFGLASIKQVGEIVVEAILNEREENGEFKSVYDFCKRV
                     DSKCSNKRVMEGLIKAGAFSNIEKSRKQLLENLEYIVATANKETQARQLGQGSLFDGL
                     QNDESFAAPQFQLTGSDEEFDDKQIQIFEKEFLGFYVTSHPLSSIRDRLPFLMTHKIS
                     EITEVPNNKMITICGLITSVKQIPTKKDPTKFIKFATIEDLTGKIEIVCFNKILQEYD
                     EFLQAEQKVIISGKVQRREEDTPNIIVETVKPVENSNIFTLELLEEISYEELVALKDI
                     LVANNGSDPVVIKIPNEGDNSRILASSLFWVNSSNDLTSAIKKQFPKKVAVYVKSLDE
                     D"
     gene            complement(6459..>6869)
                     /locus_tag="PKI94_08125"
     CDS             complement(6459..>6869)
                     /locus_tag="PKI94_08125"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNW26738.1"
                     /translation="KNSKNDNFRTLKRGQKMFTEILFSKIHRATVTDANLDYVGSITI
                     DETLIKASKLKEWQKVEILNINNGERFQTYVIKGKADSGEICINGAAARKACKGDKVI
                     IVAYGYFHSNEVEEFTPTIVLVDDKNRIVERVGK"
BASE COUNT         1862 a         1266 c         1308 g         2433 t
ORIGIN      
        1 ttgtattttt aaataaatta tttaggttct aagacctttt taacttcttt tttgtagatt
       61 tctacgttag gctgaagaac ttctgccatt gcagaacctt caggtgtttt ttcgcctgct
      121 ttttgtctta gcatattgcc tgtgtaaaga gtttcaaaat gtttaagttc aataaatctt
      181 gcgattgtct caggcgacag gtctttaaac tcaatttttg aaacaggatg ctgtgccgaa
      241 taagctgtca tcgcaccgtt tagaagtgct tctgtttttt tgtcttgagg tttaacgtag
      301 atgaaagtat agaaagaatc tttgttatta gggtccaggt cagattctgc gttatagtgc
      361 aggtaccctg gtcctatgtt tgtgtcggtt gaaatttttg atttcaaaga ttcgtttttc
      421 atctgttttt cccataaagt ggctcctgca aaagcatcac cgaagtattc gacaaagtgt
      481 ttatttcttc cgtttgattt tgcatcagcg ttgaatgccg caagttttag tgcttcgttt
      541 ttgtttatgt cggggtttaa aaattctttt tgagcggtaa gtgaggcttc aagcattttt
      601 ttggaatctt ctttgctcac gcctgcataa gccatagtga aaattgtggc atcatcaaat
      661 attgaaaatc ttccgcccac atcatcgtga acaagtcctg atgctgaaaa ttctcccctg
      721 tcaactgctt gtctaagttt gcttttttca gctgaggcgt ctgtcattgc gataaatcta
      781 tcggaaacat cttctttttt aagatgttct tgcattaatt ttcttgcact ttcgtatccg
      841 acagttgtct caagagttcc gccggatttt gaaactataa gaaattttgt tttatctaaa
      901 tccaaagaat cggcaaatct tttgaaactg tcagggtcaa tgcctgagta aaaatgaacg
      961 tgagggtctt ttccaagcat tttcatcatg gcttcgttgg tgtggcgaga gccgccgatg
     1021 ccgataacgg ctaaatctgt aaagccgcct tgtttagctt tgtttgcggt gtcatatata
     1081 gaatcaaggc tttgtaattc tgttttaggc aaaacatcaa tccagtttaa gaattgccct
     1141 ggttttcccg ctcttgaaga aatatcttca actgcaattt ttgcgggttc tttgtattgt
     1201 tctaaattca ggtttgcaac ctgaacgttt aaatttttgt tttccataaa agctgctcct
     1261 ctgtatgccc atgcaggcac atcgtttttg tattgcgtta aaagatttgt cgtatcaggt
     1321 gttttaactt ctacttgctg ctgtattgca ggtggagttt gaatgttttg aacctgtgtg
     1381 gcattttgaa aatcaatcat ttattactct cctttaaaat gtattggcag aaacttttag
     1441 taatcacacc tgaggatgcg gaaaaattaa aaatttttct cgcacctccc tagtccagca
     1501 accgccaagc tacccttcta ccaaggctat gcttggcggt tgccttcttc aggtcggata
     1561 caagaaattc tcaaaattgt atattttgga atttcttgta tcctctgtat taacatttaa
     1621 acaaaaaaaa agttgctagt aaaggctgtt ttggacacaa aaattttaca aaacaaaata
     1681 tttgggggta aacgagttga aaagtcgaac agtcgataag ttgaatagtt ttattttatt
     1741 taaatgccga aggccactct gccgaaacta aactgactaa atgtcagttt agttgagagg
     1801 ggagaaccga gcatttgtta tgtcaaatgc ttcggcgagc ggacgtgcct ttgcagaaca
     1861 caaacagacc tcccaaccgg gaggtctgtt tgtgtatttg ccgaaggccg gactcgaacc
     1921 ggcacgaggg ttgagctcgg tggattttga gtccactgcg tctaccattt cgccacttcg
     1981 gcgtacgttt agtaagttta tcatatcaaa aacacttcaa atttcaaata ttatttttac
     2041 tttttttaaa atatccttaa atatattttc aaagcggttt aattattctt tcaaatcgct
     2101 taattatgta aaaaaacaag atgaggattt attatgtttg agaatttaat tttgttgacg
     2161 gtgtttttta tgttattcgc atcacttcct gctttttcag aaagtatgca gaataaagtt
     2221 gttgtcaaag gtgcagacgg tataaacagg attattgatt caaaaaccaa taaacccata
     2281 ggtaaagcaa aaattgttgt gccgcaaagc aattatcaga cctacacgga tgagcagggg
     2341 cggtttcagc tgggggcaaa agttgatgga caaactgtta tgtcggttga aaaagagggc
     2401 tacaagccat tttctgttac acttgataaa aataacaatg ctcaagccat tgttttgggg
     2461 atagaaaaat ctactccgcg ggatattgta attgatacgg aaatgtttca cctgggggac
     2521 aataattttt ccgatgcgtc tgcaaatgca agagaattta gggtaaactc tgttggacct
     2581 ttttattcta aaaaatttaa gttgtcatct gttcctcagg ggtcttccac ttttcttgta
     2641 attggctcta ttataggcat tgacactaaa cttgcaaaat ctatggggca gaataaaatt
     2701 accaactctt atgcaagtcc gcctgaaatt tatttcaacg ggaataaaat tgcagaaatt
     2761 cagcttaacg gcgacggaca aaaaattaaa cttccaagca gtttaataag acaaaatatg
     2821 atgaacgaag ttaccataaa aacaggcaga aatttgatgc agactgctta tgtggattac
     2881 gacgacattg aatttatgaa tttaataatt gaaaacaagt aaaattaatc ttcatcaagt
     2941 gattttacat aaacggcaac ttttttagga aattgttttt taatcgcgct tgtcaaatcg
     3001 tttgaagaat taacccaaaa aagtgatgaa gcaagaattc ttgaattatc gccttcgttt
     3061 ggaattttta ttacaacggg gtctgagcca ttatttgcaa caagaatatc tttgagcgca
     3121 acaagttctt cataagaaat ttcttccaaa agttcaagtg tgaaaatatt tgaattttca
     3181 acaggtttaa ccgtttctac aattatgttt ggggtgtctt cttctcttct ttgaactttg
     3241 cctgagatta taactttttg ttccgcctgt aaaaattcat cgtattcctg caaaatttta
     3301 ttaaaacaaa caatttcaat ttttccggtc aaatcctcta ttgttgcaaa tttgataaat
     3361 tttgtcgggt ctttttttgt aggaatttgt ttaacagaag ttataagccc gcaaattgtt
     3421 atcattttgt tgttgggaac ttctgtgatt tctgaaattt tgtgagtcat caaaaaagga
     3481 agcctgtctc tgatgcttga aagcgggtgg ctggttacat aaaatcctaa aaattctttc
     3541 tcgaaaattt gaatttgttt gtcgtcaaat tcttcgtcgc ttcctgtaag ctgaaattga
     3601 ggagcagcaa agctttcatc gttttgcaag ccgtcaaata aacttccctg cccgagctgt
     3661 cttgcctgcg tttctttgtt tgctgttgca acaatgtatt ccaaattctc aagaagctgt
     3721 tttctgcttt tttcaatgtt tgaaaaagct cctgctttga ttaaaccttc cataaccctt
     3781 ttgttggaac attttgaatc aacccttttg caaaaatcgt aaaccgattt aaattctccg
     3841 ttttcttctc tttcgtttaa aatcgcttca acgacgattt caccgacttg ttttattgat
     3901 gcaagtccga atctgatatt tttttcatcg ggcgtaaatt cggcgaatga cttatttatg
     3961 tcaggcggta aaacttttat tccgtatttt agagcttctt caatataggc ttgggtttta
     4021 tcttggtcat tggctacact tgaaagcaat gccgataagt attctactgt gtagtgtgtt
     4081 tttaaataag ccgtctgata tgcaacaaaa gcgtaagccg cagagtgcga cctgttaaaa
     4141 cagtattcgg caaatttttc tatctgttcg aaaagttcgg tcgcttcttt tggcatcatg
     4201 ccgtttttgc ctgcgccttc aatgaacttt tctttttgtt ttgccatttc ttcgactttt
     4261 ttcttaccca tcatgcggcg aaccatgtcc gcttgtccga gcgaataccc cgcaagcgtt
     4321 tggaaaatct gcataatttg ttcctgataa acaatcgttc cgtaagtgtc tttcaaaatc
     4381 ggctcaagca aagggtggtc gtatgtgatt gcctgtctgc cgtgttttct ttgaacaaag
     4441 tcttctacca tgccagattc caaagggccg ggcctgaaaa gtgcaacgag tgcgcctaaa
     4501 tcttcaaaaa cgcttggttt taaatctctt acaagacgct tcatccctgt ggattcaagc
     4561 tgaaaaacgc cgtctgtttc gcctcgggtg agcatttcgt aagttttttt atcatcaagt
     4621 ggaatgttat ttatatcgat atcaaggccg tgtcttgttt taatcatttt gatggttttg
     4681 cttatgattg taaggttgcg cagccccaga aaatccattt ttaaaagccc gagtttttca
     4741 agatcggtca taggatattg tgttattata ctgccgtctt ttgagggttg aacaggtaca
     4801 atttcgttaa gcggtttgtg agaaattatc acacctgcag cgtgcatgcc gatattgttt
     4861 ttgacccctt ccaatccttt tgccatgtca acaagttttt tgatttcagg gtcgttatcg
     4921 tataaaattt ttaattccat gccctctttt agtgcatcat ctatttttgc acccggagtt
     4981 gagggaatta gtgcagaaag tttgttgctc tctgcaaaag gaatattaaa aactcttgca
     5041 acacctttaa gcgcggcttt tgcggcaagc gttccgaagg ttacgatttg gcatacatgg
     5101 tcttcgccgt atttttttga aacgtaatcg attacttcag agcgtctttc gatacaaaaa
     5161 tcaatatcca catcgggcat gcttattctt tcggggttca aaaatctttc aaacaaaaga
     5221 ttgtgccgga tagggtctaa atctgttatt tcaagggcgt aagcaacaat actccctgcg
     5281 gcagagcctc tgccgggacc gacggggatc ccgttttgtt tggcgaaatt tataaaatcc
     5341 cacgtgatta aaaagtaagc cgaaaacccc atttgttcaa tgatttcaag ttcatactta
     5401 accctttttt caatttcggg ggttatttct ttgtagcgtt tttcaagacc ttctctgact
     5461 ttaaagtcaa gataactttc tgtcgtgtgg ttttttggca cttcatattt tggaagcaaa
     5521 ctgcctccca tttcgattat caaatggcat ttttcggcaa tttcaaccgt atttttaatt
     5581 gctttttcaa aggtttcact gtcaagccac ttaaaagaat cccgaagttc tgaaacggtt
     5641 tttacgtaaa attcgttgtt tgggaaatga aaacggtttt catcggtttt gagggcgttt
     5701 gtctgcagac acaaaagagt gtcatgccag tcggcgtctt ctttttttaa ataatgtgag
     5761 tcgtttgtaa ttacaatttt aattcccagt tcttttgcaa ggtttatcaa atcagggttt
     5821 gagcgttttt gtttgtcaag cccgtggtct tgaagttcaa tgtagtaatc ttcgccgaaa
     5881 agttctttat attttaaagc cctttctttt gcggctttta tatctttttg caggattaaa
     5941 tccgcaagct cgccctgaac gcatgatgac agacaaatta agccttgatg atactgcttt
     6001 ataagttcaa aatttactct gggtttgtaa taaaatcctt cacagtgtgc tatcgagacc
     6061 aattttacaa gattcgcata tccctgtttg tcttttgcta ttaaaacaag gtgattcagg
     6121 tgggttttag attggttttt ttctgaaatg tcgccttggt aaacataaaa ttcgcagccg
     6181 attaaaggct taaccccaca gtcttttgcg gttttgtata agtcaatcgc accgtacatt
     6241 gctccgtggt ctgttacggc aacggcgggc atgtcatttt gctttgcaaa ttcgcaaagt
     6301 tcttttattt ttatcgcccc gtcaagaagc gaatattcac tgtgtaagtg taatggtacg
     6361 tattgcatta tttcaggtga ataggtgaat aggtgaatag gtgaataaga aaaaatcata
     6421 taaaatgcac ttattcactt atttacttat ttacttattc actttcccac cctttcaact
     6481 attctattct tatcatcaac cagaactatt gtaggtgtga attcttctac ttcattcgaa
     6541 tgaaaatatc cgtaagcaac aattatgact ttatcgcctt tgcatgcttt tcttgccgct
     6601 gcaccgttta tgcagatttc gccagaatca gctttacctt ttataacgta agtttgaaat
     6661 ctttcaccgt tgtttatgtt taaaatttca accttttgcc attctttaag tttagaagct
     6721 ttaatcagtg tttcgtcaat agtgattgag ccgacgtaat caagattagc gtcagttacg
     6781 gttgccctgt gtatttttga aaataaaatt tctgtaaaca ttttttgtcc tcttttgagc
     6841 gtgcgaaaat tgtcattttt tgaattttt
//
LOCUS       DAOQAU010000070         6763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_13748_length_6763_cov_6.453936, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000070 DAOQAU010000000
VERSION     DAOQAU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13748_length_6763_cov_6.453936"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..516
                     /locus_tag="PKI94_08130"
     CDS             184..516
                     /locus_tag="PKI94_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNW26739.1"
                     /translation="MNMMQMMQQAQNLQKKLKAAQEELANSEVTGESAGGAVKVVCDG
                     QGKFKSIKLSPEAINPENPSSVDADTVEMLEDIITTAILQASNKATAEMETKMKALTG
                     GINIPGLF"
     gene            619..1218
                     /gene="recR"
                     /locus_tag="PKI94_08135"
     CDS             619..1218
                     /gene="recR"
                     /locus_tag="PKI94_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNW26740.1"
                     /translation="MIYPKSIAALIEQFQKFPAIGPKSAQRMAFHLLKMPMPEVQKIA
                     QALVEAKENTFACDICFNMSSTNPCEICSSSGRDRTMICVVSETKDLIAIEKTNEFKG
                     LYHVLQGLISPIDGIGAEDIRIKELLHRLANEEVKEVILALSPSVEGEATSLYLSKLI
                     KPFGITISRIAFGLPVGTDLEYADEITLARALEGRREIN"
     gene            1234..1815
                     /locus_tag="PKI94_08140"
     CDS             1234..1815
                     /locus_tag="PKI94_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26741.1"
                     /translation="MKRFFLTILFLFVFALSASAKTQTVKVMALSDFSTQNPSPTFRA
                     EIIEGEIFKGDFLDSGTIVEGRVIRIESPKFGKQNAYFVFLPYVLTQNGKTTTTKEPM
                     AVAKVVGYSPVDTKKLVGKVFKTAANFVVKGASLGISFVEGAAKAQEGDRFQSGLVNV
                     YNDSPLSYIEPGKELNIEPGDILILKLSEFSTY"
     gene            1903..2877
                     /locus_tag="PKI94_08145"
     CDS             1903..2877
                     /locus_tag="PKI94_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW26742.1"
                     /translation="MENLLEIKNLFVEYKMEKEFSQGQKTVFAVNDLSLDIKKGEILA
                     IAGESGCGKSTLAKAILKLIESKSGGIFLGTGDNVLKLKQNDLKKFRQNAQIIFQNPF
                     SSLNPKMKIYEILKEPLIVNKNSPALKNITDAEMDKRIKDTIKQVGLDEKALYLYPHE
                     FSGGQRQRIAIARALILQPEFIVADEPVSALDVSIQAQIINLLQELKEKFNLTFLFIS
                     HDLSVIKYLADRVAVMYLGEIVELGTSAEIFNSPQHPYTKALLSAAPGFKKDEKIILS
                     GDLPSPTDLPKGCKFHTRCPYVMDICKIQAPETKNFSQSHCANCHLYD"
     gene            complement(2926..3135)
                     /locus_tag="PKI94_08150"
     CDS             complement(2926..3135)
                     /locus_tag="PKI94_08150"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNW26743.1"
                     /translation="MQETLEQIGFSAGQIYNYLSENNGKSTFTKMKKDLDLKGNFAEL
                     GLGWLAREDKIDITKKGASINVVLR"
     gene            complement(3246..5129)
                     /gene="mnmG"
                     /locus_tag="PKI94_08155"
     CDS             complement(3246..5129)
                     /gene="mnmG"
                     /locus_tag="PKI94_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNW26744.1"
                     /translation="MEYLKNKFDTIVVGAGHAGCEAALAAANLGAKVLLCSLNLDNIA
                     LMPCNPAIGGPAKSTLVREIDALGGVMAEVTDATYIQMKMLNSSKGPAVRALRAQSDK
                     KEYMAYMRKLLEENKNIYLKQCCISRLLVEKGKIKGAVDQYGIEYFAPTVILTTGTSL
                     EGKIFVGLKSYSAGRLGEMPATGLSDCLKTLDFTVKKLKTGTPARVDSRTIDYSKMTI
                     QPGDEELNFYSFKPDRPIREQIPCYLTRTNEKTHQIIKANLDKSPMYQGLIEGVGPRY
                     CPSIEDKIVRFSSNPSHHIFIEPEGLNTYEVYVQGFSSSLPADVQIQMLRTLPGLENV
                     HVIKPAYAVEYDYVPAIQLNHTLMTRLIQGLFNAGQINGTSGYEEAAAQGLIAGINAV
                     KFINNEEMVELSRESSYIGTLIDDLVTKDIQEPYRMLTSRSEYRLLLRQDNADARLIP
                     LGHKIGLISDKQFEHFQQKQKSIENEIKRLSKEKISVNEKTNSILAKYDHNIERGIKL
                     IDLLKRPGIDYKILKELDEKTEELNLPREVYEQVEILTKYDGYLKRQEFHVEQATKLE
                     KFRIPENIDYMSIKHISTETKEQLNKIRPKTLAQASRIGGVKPADISVLMVILEKGLV
                     LNN"
     gene            5237..5833
                     /locus_tag="PKI94_08160"
     CDS             5237..5833
                     /locus_tag="PKI94_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26745.1"
                     /translation="MKKVLFSVLFMFLMLPAFSQTIEGGVTFDWISKSQLQRDENIKQ
                     VQNVLFTDKTIYKYPKKEFKAEYAAVWKDKDHLKTYEEVLRGKKEDADNYYCGFNLKN
                     GLLIAYGIQPKKDMKHIYYYDAMGHLIFVDNFSSAYPKFPYESSQYYKNGELVAKYYY
                     VSDQDQYVFDAKRKFKGRWYKEKMYNRNAKVIMTRSNY"
     gene            complement(5888..>6763)
                     /locus_tag="PKI94_08165"
     CDS             complement(5888..>6763)
                     /locus_tag="PKI94_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNW26746.1"
                     /translation="YDNPNAYNGLRDSDCVISVGLINSDLNTYRLGLPFKPTDFIDIQ
                     GHYTIVENKKYENVLMKDMLQELTKRIKPRVRELPEKIPSFEKVKPAKATPLSINYVF
                     PRLQDFFKPDDMIVVETGIIPHGFAPTRLPKNTEVNTQTLWGSIGWATAATFGVQMAA
                     KDRRTILITGEGSHQLTAGEVSSMMRHKLKPIIIVINNSGYTIERVLSDDPWDPFNEI
                     VKWDYSKLPTVFEGDVWIAQARTDKEFDEVLNQAEIEQKSRLCYIEIFTGKMDLPELT
                     QKVVESIRGKKIKNK"
BASE COUNT         2005 a         1201 c         1339 g         2218 t
ORIGIN      
        1 cttcaagctt ttttgaataa aatctaaaag gggcaatgta gaattttaag ggcattaaat
       61 tgtctgccca gcgcataatt ctgccgtttc gtaaactttc ttgtaaataa gttttctttt
      121 gcataaattt attttataat tattttctga acaaatcaag aaaattaagg ggagaatgaa
      181 tttatgaata tgatgcaaat gatgcagcag gctcaaaatt tacaaaaaaa acttaaagcg
      241 gctcaagaag agcttgccaa ttccgaagtt acaggggaat ctgccggagg ggcggtaaag
      301 gtggtttgtg acggacaggg taagtttaaa tccattaagc tcagccctga ggcgataaat
      361 cctgaaaacc cttcttctgt tgacgctgat acggtagaaa tgctcgaaga tattattaca
      421 accgcaattc ttcaggcaag caacaaagca actgccgaaa tggaaactaa aatgaaggct
      481 ttgacaggcg gaattaatat tcccggattg ttttaagggg tcgtaatttc acctcgcccc
      541 ttgagggaga ggtctgtttt tgatatgagc aagccttgcg aatatctaaa aacagggtga
      601 ggggtaagag gagcaccaat gatttatcca aaatccatag cagcattaat cgagcagttt
      661 caaaaatttc ctgcaatagg tccgaaatct gcacagagaa tggcatttca tcttttaaaa
      721 atgcccatgc cagaggtgca aaaaatagcg caggcgcttg ttgaggcaaa ggaaaatact
      781 tttgcatgcg acatttgctt taatatgtca tccacaaacc cttgcgagat atgctcgtca
      841 tcagggcgtg acagaacaat gatttgtgtt gtttctgaga caaaagattt aattgcaatc
      901 gaaaaaacaa atgaatttaa agggctttat catgtgcttc aggggcttat ttcgcccatt
      961 gacggaatag gcgcggaaga catcaggata aaagaacttt tgcacaggct tgcaaatgag
     1021 gaagttaaag aggtaattct tgccctcagt ccttccgtgg aaggtgaagc gacaagcctt
     1081 tacctttcaa aactgataaa acctttcggt ataaccataa gcagaatcgc tttcgggctt
     1141 cctgtgggaa ctgatctgga atacgccgat gaaatcactc ttgccagagc tcttgaaggc
     1201 agaagagaaa taaattagta ttttattgcg atgatgaaaa gatttttttt aacaatttta
     1261 tttttgtttg tctttgcgct ttcggcaagt gcgaaaaccc agacggttaa ggttatggct
     1321 ttaagtgatt tttcgacgca aaatccttcc ccgactttta gggcagaaat tattgaagga
     1381 gaaatcttta agggtgattt tcttgactcg ggcactattg tagaaggaag agtaataaga
     1441 attgaaagtc ctaaattcgg aaagcaaaac gcttattttg ttttcctccc ttacgtttta
     1501 acacaaaacg gaaaaaccac gacgaccaaa gagcctatgg ctgttgcaaa agttgtcggt
     1561 tattctccgg ttgatacaaa aaaacttgtc ggaaaagtct ttaaaaccgc cgctaatttt
     1621 gtggtcaaag gtgcttcttt gggaatttct tttgtggaag gcgctgcaaa ggcgcaagaa
     1681 ggcgacagat ttcagtccgg tttggtaaac gtttataatg attcgccttt gtcttatatt
     1741 gagccgggca aagaactgaa tattgaaccc ggtgatatac ttattcttaa gctgagtgaa
     1801 tttagtactt attaattatt actccgcaat gatgaccatt ctcggttgaa tttatttata
     1861 attaatagta aaatgctttt tgtttacttt tgcgaggtgt ttttggaaaa tttattagaa
     1921 attaaaaatc tttttgttga atacaaaatg gaaaaagaat tttcgcaagg acaaaaaacc
     1981 gtttttgcgg ttaatgattt gtctttggat attaaaaaag gcgaaatcct tgcaatagca
     2041 ggcgaatcag gctgcggaaa atccaccctc gccaaagcaa ttcttaaatt gattgaatca
     2101 aaatcgggcg gaattttttt aggaacaggt gataatgttt tgaaacttaa acaaaatgac
     2161 ctgaaaaaat tccgccaaaa tgctcagata atttttcaaa acccgttttc aagccttaat
     2221 cctaagatga aaatttatga aattttaaaa gaacctttaa ttgtgaataa aaattcaccg
     2281 gctttaaaaa acattacaga tgccgaaatg gataagagga taaaagatac aataaaacaa
     2341 gtcggcttag atgaaaaagc tttatacctt tacccccatg aattttcagg cgggcaaaga
     2401 caaaggattg caatagcaag agcattaatt ctccagcccg aatttattgt cgcagatgag
     2461 ccggtaagtg ctttagatgt gagtattcag gctcagataa taaatctttt gcaggagttg
     2521 aaagaaaaat ttaatcttac ttttcttttt atttcgcatg atttaagtgt tataaaatat
     2581 cttgcagaca gagtggcagt tatgtatctt ggtgaaattg tcgagctggg aacaagcgcg
     2641 gaaattttta attctccgca gcatccgtac acaaaagccc tgttgagtgc agctccggga
     2701 tttaaaaaag atgaaaaaat aattctcagc ggtgatttgc cttctcctac cgatttgccc
     2761 aaaggctgta aattccacac caggtgccct tatgttatgg acatttgtaa aattcaagct
     2821 cctgaaacga aaaatttttc gcaatcacat tgtgctaatt gtcatttgta tgattaaaaa
     2881 caaaaaaggg cggtgctttc agcaccgccc ttttttgttt aaaacttatc tgagaactac
     2941 gttaattgaa gcaccttttt tagtgatgtc aattttgtct tctctggcta accagccaag
     3001 accgagctca gcgaaatttc ctttaaggtc taaatctttt ttcatttttg tgaaagttga
     3061 ttttccgttg ttttcagaaa ggtagttata aatttgtcct gctgaaaatc cgatttgttc
     3121 taaagtttct tgcatatttc tatctccttt gtgtaaataa aaagtaaatg taactataaa
     3181 atttatacta taacaaagaa aatcaaattg caatatccga aaaatcctac taacggttga
     3241 tttatctagt tattaagtac taatcctttt tccagaatta ccattaaaac ggaaatatcg
     3301 gcaggtttta ctccgcctat tcttgaggct tgagctaaag ttttggggcg gattttgtta
     3361 agctgttctt tggtttccgt tgaaatatgt ttaattgaca tgtaatcaat attttcaggg
     3421 attctaaatt tttcgagttt tgttgcctgc tcgacatgaa attcctgacg tttaagatat
     3481 ccgtcgtatt ttgttaaaat ttcaacctgc tcgtaaacct ctcttggcaa attaagctct
     3541 tcggtttttt catcaagttc tttcaaaatt ttataatcaa tgcccggtcg tttgagaagg
     3601 tctattaatt taattccgcg ttcaatgttg tggtcgtatt ttgcaagaat tgaatttgtt
     3661 ttttcgttaa cagaaatctt ttcttttgaa agccgtttaa tctcattttc tatgcttttt
     3721 tgtttttgct gaaagtgttc aaactgcttg tcacttatta atccgatttt gtgtcccagt
     3781 ggaatcaatc tggcatctgc attgtcctgt cttaataaaa gcctgtattc agagcgagat
     3841 gtcagcattc tgtagggctc ttgaatgtct tttgttacca aatcgtctat cagtgttcct
     3901 atataggaag attctcttga aagttcaacc atctcttcgt tgttgataaa ttttacggcg
     3961 ttaattcctg cgataagacc ttgtgcggca gcttcttcgt agccgcttgt tccgtttatc
     4021 tgtcctgcgt taaaaagccc ctgtataagc cttgtcataa gcgtgtggtt aagctgaatt
     4081 gcgggtacat aatcgtattc aacagcataa gccggtttaa tcacatggac attttccaat
     4141 ccgggaagag tgcggagcat ttgtatttga acatcggcag gcaggctgct tgaaaaaccc
     4201 tgaacataaa cttcataagt gttaagtcct tcaggctcaa taaaaatatg atgcgaaggg
     4261 ttagaagaaa atctcacgat tttgtcttcg attgaaggac aatagcgggg gccgactcct
     4321 tctataagtc cttgatacat aggggattta tccaaatttg cttttataat ttggtgtgtc
     4381 ttttcgtttg ttctagttaa ataacaagga atttgttctc ttatcgggcg gtcgggttta
     4441 aatgaataaa aatttaactc ctcatcaccg ggctggattg tcattttcga ataatcaata
     4501 gttcttgaat ccactcttgc gggagttcct gtttttaatt ttttgaccgt aaaatctaaa
     4561 gtttttagac agtcagaaag tcctgtggca ggcatttctc cgagcctgcc tgcactatag
     4621 gattttagtc ctacaaaaat tttgccttcc agtgaagttc ctgtggttaa aattacggtc
     4681 ggtgcgaaat attcgattcc gtattggtca acagcaccct taattttgcc tttttccacc
     4741 aataaccttg aaatacagca ctgttttaaa tagatatttt tgttttcttc aagcagtttt
     4801 cgcatgtaag ccatgtattc ttttttgtcg gattgggcgc gaagtgctct aacggcaggc
     4861 ccttttgagg aattaagcat tttcatttgg atgtaggtgg catctgtaac ttctgccata
     4921 acaccgccca gcgcatctat ttctcttacc agagtagatt ttgcaggacc gccgattgca
     4981 gggttgcagg gcataagcgc aatgttatca aggttaagcg agcacaaaag aacttttgcg
     5041 cctaaatttg ctgcggctaa agccgcttca cagcccgcgt gtcctgcccc tacgacaatt
     5101 gtatcaaact tgttttttaa atattccata atataattat agtataaaaa agtgagcagt
     5161 gagcggtgag ctgtgaactg agtaggttgg gctttccagc ccaacaaatc ggagtaatga
     5221 tttattgagg tgagttatga aaaaagtttt attttctgtt ttgtttatgt ttttgatgtt
     5281 accggcgttt tctcaaacaa tagaaggcgg agttactttt gactggattt ctaaatctca
     5341 gcttcagcgt gatgaaaata ttaagcaggt tcaaaatgtt ttgtttaccg acaaaactat
     5401 atataaatat cccaaaaaag agtttaaggc agaatatgcc gctgtttgga aagataaaga
     5461 tcacttaaaa acttacgaag aagtgcttag aggtaaaaaa gaagatgcgg ataattatta
     5521 ctgtgggttt aatttgaaaa acgggttact tattgcttat ggcattcagc ctaaaaaaga
     5581 catgaaacat atttattatt acgatgctat gggacacttg atttttgtgg ataatttttc
     5641 ttcagcttat cctaaatttc cttacgaatc atcccaatac tacaaaaacg gtgaattggt
     5701 cgctaaatat tattacgttt cagatcagga ccaatacgtg tttgatgcca aaagaaaatt
     5761 taaaggacgc tggtacaaag aaaaaatgta caaccgcaat gcaaaagtta ttatgacaag
     5821 atcgaattat taatattaaa aaaggggcgg attttcgaag aaaatccgcc ccttttttaa
     5881 ttaaattcta tttattttta atttttttac cacggatact ttcgacgact ttttgcgtga
     5941 gttccggtaa atccattttt ccggtaaaaa tttcgatgta acaaagtctt gatttttgtt
     6001 cgatttctgc ttgatttaag acttcgtcaa attctttgtc tgttcttgcc tgagcaatcc
     6061 aaacatcgcc ttcaaagact gtgggcaatt ttgagtaatc ccatttaact atttcattga
     6121 acggatccca cgggtcatct gaaagcactc tttcaatagt atatccgctg ttatttatga
     6181 caattattat cggttttaat ttatggcgca tcatgctgct tacttcgcct gccgtgagtt
     6241 ggtgcgagcc ttcgcctgtt attaaaattg ttcttctgtc ttttgccgcc atttgaactc
     6301 cgaaggttgc agctgttgcc cagcctattg agccccaaag cgtttgggtg tttacttcgg
     6361 tgttcttagg tagccttgtc ggcgcaaagc cgtgaggaat aattcccgtt tcaacaacaa
     6421 tcatgtcatc aggtttaaaa aaatcctgaa gccgcgggaa aacgtagttt atgctcaaag
     6481 gtgtagcttt tgccggttta accttttcaa aactcggtat tttttctggc aattctctaa
     6541 ctctcggttt aattcttttg gtgagttctt gaagcatgtc tttcatcaaa acattttcgt
     6601 attttttgtt ttccactatt gtgtaatgac cttgaatgtc aataaaatca gtcggtttaa
     6661 atggaagtcc gagcctgtat gtgtttaaat cgctgtttat aagtccgacg gaaatcacac
     6721 agtcagaatc cctaagcccg ttgtaggcat tggggttatc gta
//
LOCUS       DAOQAU010000071         6500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_14545_length_6500_cov_7.750039, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000071 DAOQAU010000000
VERSION     DAOQAU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14545_length_6500_cov_7.750039"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="mraY"
                     /locus_tag="PKI94_08170"
     CDS             <1..927
                     /gene="mraY"
                     /locus_tag="PKI94_08170"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNW26747.1"
                     /translation="LLGVPYIDFLKTKMFGQYIKDVAPQGHAQKSGTPTTGGVFIIAA
                     IILASVITLLLAQKLNTNALIVLITLLFYTLAGFQDDFLKIKGKANKGLSARGKFLRQ
                     IAIALLPALFVTLAGRTFVQVGYDYLYLYWFYPILAVFLVTGFSNAFNLTDGLDGLAA
                     SCGIPSFIACVVILEVFKYHEMAIIAAAVVGALIGFLRYNKPKAQVFMGDTGSLALGG
                     LLGTLAVMGKFEFYLIIFAAVFVIETLSVMIQVASFKTTGKRVFKMSPIHHHFELEGW
                     SEKQIVARFALISLLFSILAIVLFNAFYWGWV"
     gene            956..2305
                     /gene="murD"
                     /locus_tag="PKI94_08175"
     CDS             956..2305
                     /gene="murD"
                     /locus_tag="PKI94_08175"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNW26748.1"
                     /translation="MQNWVEKKVLILGLSKSGIAAAKYLRKKGAEVFITELREQKPED
                     KNTIFELEKLGIKVEMGGHSDKFIENSSIAVTSPGIPPKAEIFKKLKERKIHVISEVE
                     LAYEETSKPFVAITGTNGKTTTTALTAQILNQEYKAQACGNIGSPPCDYLEKDVEYFV
                     CEMSSYQIATSTVFQAQIACWLNFTPDHLDWHDGVDNYFDAKAKLFLPPQSPIFAVFN
                     GADEKLLEFSKKVPSQVFLFDKETSDNCCYIKDAAIFFKRNRQEEKIIDLKDCPLVGH
                     HNYQNIMCSVIVAKLVGIENKNIATAIKSFKAPEHRLEYVAQVNGIKFYNDSKATNPE
                     AAIVAIKSFDKNTVLIAGGRDKNTDLGEFCEEVKKHINTVILIGEAGERFATNLKERG
                     FDNIIFESTLESAIDKSMELKPEVVLLSPACASFDMFKSYEHRGEVFKDYVLFKIHK"
     gene            2280..3467
                     /gene="ftsW"
                     /locus_tag="PKI94_08180"
     CDS             2280..3467
                     /gene="ftsW"
                     /locus_tag="PKI94_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNW26749.1"
                     /translation="MSYSRYTNKKPRQYEPQMSVIISPPDKTLLVVVAFLVILGLMAI
                     FSASAPKCMDAGLNPAKFLLSQMVYLVVGYIGLKFLTNYDYKNLVLITTPLAFFVILF
                     LILVNYTPLGVTVNEAKRWIGIGMFQFQPSEFAKPAVIMLLASAFYKNDNIMDNHKIV
                     YYFLPILLMVFMIFKQPNLSMVILLMLTSLVMYLSAGGPLKVFVMGAGIGAFGLLTAG
                     VTQLIKPYQMSRITTWLNPESDPLGAGYNVIQSMVAFATGGFWGVGYGSSKQKLAWLP
                     ESHTDFIFAVMAEELGFIGCLLVIGLFWTFIHRGFLIASRCPDMYGKLLAVGITFSIG
                     FQAFLNMSVASSLLPATGVTLPFISYGGSSLMVSMWMVGILLNISKKRVKKIRVNANV
                     RHL"
     gene            3451..4596
                     /gene="murG"
                     /locus_tag="PKI94_08185"
     CDS             3451..4596
                     /gene="murG"
                     /locus_tag="PKI94_08185"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNW26750.1"
                     /translation="MSDTYDKKNKYIYFITGGGTGGHIYPAIAVFDALKNDPDTKEIY
                     YVGNPDNLEFEITKKAGYKFLGVKISAMPRKIGFLGLNFLIWFFQLQAATFKALFYIK
                     KYKPDAIFGTGGYVSAPALFAANLADVPFMIHDCDVKPGILSRYISPMAKTVSLAFEG
                     AKEYMKSQNINVNGNPIRESFFESSKKEAREKLGIQDKMTLCIMGGSQGAKSINTAAV
                     GVLKYLCEQRDVQIIFQTGKKKFDEVTALLKEIYPEFESNKNLILKPYIDDMASVLKA
                     SDIAVSRAGSLSLSELCASGVASILIPYPHAAADHQRKNADFMVKERAALYLEDADTT
                     KDTLLIAIEQILDSQEKLKIIQENAFALAKPKATKDIVGQLKGIIFE"
     gene            4589..6277
                     /locus_tag="PKI94_08190"
     CDS             4589..6277
                     /locus_tag="PKI94_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNW26751.1"
                     /translation="MSKQKYNKAIGILTSQGRFHIKLGLERVEKILEYLGNPQKKLKF
                     IHVAGTNGKGSVCAMLSAILSNIEPAPLEGEGQGGGYYKISRLYSKKTLEHAKCLRKN
                     MTDAENILWYYLRKKQMCGFKFRKQSPIGSYIADFVCFEKKLIIELDGGQHLENNSIK
                     YDEVRGNFLKKAGFDVLRFYNTDIFKNIESVLECIFLYLNPPTPNPPPQGGREQNFTP
                     HNNNCFSINYNKQALRSSVLKVGLFTSPHVFEYTERIKINDAQIDKKTFAQEIIKITE
                     LAQKNNIALTEFEILTAVAFEYFAQNNVDLVILETGLGGRFDATNIIKKNICSIITSI
                     DFDHTERLGDTIEKIAFEKAGIIKSGSFVLINKNNLGFEVIKKIAKEKRTQVLTADAG
                     RNFPQENSALQGNYQKENLSLVLKTIEFLNKNGFKICKKDIQKGLEKFKHPCRFEFHT
                     ENILIDAAHNPSGAKALRKSLEKKFPNQNFRFIFGCLRNKNYPQMIENLFSPEDEIFF
                     YQFDNPNSCSFEELNAVCPFAAKSFKKFNKSDKITVICGSIYMIKELLTKIDKK"
BASE COUNT         2152 a         1060 c         1288 g         2000 t
ORIGIN      
        1 ttgttgggag tgccttatat tgattttctt aaaacaaaaa tgtttggtca atatataaaa
       61 gatgtagctc cgcaagggca cgctcaaaaa agcggaacac caacaacagg tggggttttt
      121 ataattgcgg caataatttt ggcatctgtt ataacacttc ttcttgccca gaaattaaat
      181 acaaacgctc ttattgtgct tataactttg cttttttata ctcttgcggg atttcaggat
      241 gattttttga aaataaaagg caaagcaaat aaaggattat cggcacgcgg caagttttta
      301 agacagattg ccatagcact tttgcctgca ctttttgtaa cattagcggg aagaactttt
      361 gttcaagtcg gatatgatta tctttatctt tattggtttt atccgatttt ggcagtgttt
      421 ttggtaactg gcttttcaaa tgcttttaat ctgacagacg gacttgacgg gcttgcggct
      481 tcttgcggaa ttccttcttt tattgcctgc gtggtgattt tggaagtttt taaataccat
      541 gaaatggcaa taattgcagc agcggttgtg ggtgctttga taggcttttt aagatacaac
      601 aaacctaaag cacaagtatt tatgggggat acaggttctc ttgcacttgg cggattgctt
      661 ggaacgcttg ccgttatggg gaaatttgag ttttacttaa ttatttttgc ggcggttttt
      721 gtcattgaaa ctctttctgt tatgattcag gtcgcaagtt ttaaaacaac aggaaaaaga
      781 gttttcaaga tgtctccgat acatcatcac tttgaacttg agggctggag cgaaaaacaa
      841 attgtggcaa gatttgcctt gatttcgctt ttgttctcaa tacttgcaat tgttttattc
      901 aacgcatttt actggggttg ggtataattt tgctatggaa aattgaggga ttgtaatgca
      961 aaattgggtt gaaaaaaaag ttttaatatt gggtttgtca aaaagcggga ttgcagcggc
     1021 aaaatacttg agaaaaaagg gtgctgaagt tttcataacg gaattaagag aacaaaaacc
     1081 ggaagataaa aacaccattt ttgaacttga aaaattggga ataaaggttg aaatgggggg
     1141 gcattcggac aagtttatag aaaattcttc cattgcagtt acaagccccg ggattccgcc
     1201 taaagcagaa atttttaaaa agcttaaaga aagaaaaatc catgtgattt cagaggtaga
     1261 acttgcatat gaggaaactt caaaaccttt tgttgcaata acaggaacta acgggaaaac
     1321 caccacaaca gcacttacgg cacaaatttt aaatcaggaa tacaaagcac aagcctgcgg
     1381 aaatatcgga agcccgcctt gcgattatct tgaaaaagat gttgaatatt ttgtttgtga
     1441 aatgagttct tatcaaattg caacaagcac cgtgtttcag gcgcaaattg catgctggct
     1501 taattttaca cctgatcatc ttgattggca cgacggggtg gataattatt ttgatgcaaa
     1561 agcaaaactt tttttgcctc cgcaaagccc tatttttgct gtttttaacg gagcagacga
     1621 aaaacttttg gaattttcaa aaaaagttcc ttcgcaggtt tttctctttg acaaagaaac
     1681 ttcagataat tgttgttata taaaagatgc ggcaatattt tttaagcgca acaggcagga
     1741 agaaaaaatt atcgatttaa aagactgtcc tttggtcggt caccacaatt accagaatat
     1801 aatgtgctcg gttattgtgg caaaacttgt gggaattgaa aataaaaaca tagcaactgc
     1861 cataaaatct tttaaagcac ccgagcacag acttgaatat gttgcacaag ttaacgggat
     1921 aaaattttat aacgattcaa aagcaacaaa ccccgaagct gctatcgttg cgataaaatc
     1981 ttttgacaaa aacaccgttt taattgcagg cggaagggat aaaaataccg atttgggcga
     2041 attttgcgaa gaagtaaaaa aacatataaa tactgttatt ttaataggag aagcaggtga
     2101 acgttttgca acaaacttga aggaaagagg ttttgataat ataatttttg aaagcacgct
     2161 ggagagtgca atcgataaat caatggagct taagcctgaa gttgtacttt tatcgcctgc
     2221 ttgcgcaagt tttgatatgt tcaaaagcta cgaacacaga ggggaagtat ttaaagacta
     2281 tgtcttattc aagatacaca aataaaaaac ccagacagta tgaacctcaa atgagtgtca
     2341 ttatttcacc gccggataaa actcttttgg tggtggtagc gtttcttgta attttaggtt
     2401 taatggctat attttcggca agtgccccaa aatgtatgga cgcagggctt aatccggcta
     2461 aatttttatt gagccaaatg gtttatcttg tcgtcgggta tatcgggtta aaatttctaa
     2521 caaattatga ttataaaaat ttagttttaa ttacaacacc gcttgccttt tttgtcatac
     2581 tctttttgat tttggtaaat tacactcctt taggggttac cgtaaatgaa gcaaaaaggt
     2641 ggattggaat aggtatgttc cagtttcagc cttcggaatt tgcaaaacct gctgtgataa
     2701 tgcttcttgc aagcgcattt tacaaaaacg acaacattat ggacaaccac aaaattgttt
     2761 attattttct gccgatatta cttatggtat ttatgatttt caaacagccc aacttgagca
     2821 tggtaatttt attaatgttg acttcgcttg taatgtattt aagtgcaggc gggcctttga
     2881 aggtttttgt aatgggtgcg ggaatcggtg ctttcggact tttaaccgca ggagttactc
     2941 agcttataaa gccttatcag atgtcaagaa ttacaacctg gcttaatccc gaatctgacc
     3001 ctttgggcgc aggttataat gttattcaat caatggtagc ttttgcaact ggcggatttt
     3061 ggggtgtggg ctacggaagt tccaaacaaa aacttgcatg gctaccagaa tctcacaccg
     3121 actttatctt tgcggttatg gcggaagaac tcggttttat cggctgtctt ttggtaatag
     3181 ggcttttttg gacttttatt cacagaggtt ttttaatagc gtcaagatgc cctgatatgt
     3241 acggaaagct tcttgccgtt ggaatcactt tttcaatagg atttcaggca tttttgaata
     3301 tgagcgttgc aagctcgctg ctgcctgcaa caggtgttac gcttcctttt ataagctacg
     3361 gcggatcttc gcttatggtt tcaatgtgga tggtaggaat tttgttgaat atttccaaaa
     3421 aaagagtgaa aaagattagg gttaatgcaa atgtcagaca cttatgataa aaaaaacaaa
     3481 tatatttact ttataacagg cggtggtacg ggcggacata tttatcctgc aatagcagtt
     3541 tttgacgcgc tgaaaaacga tcctgacaca aaagaaattt attatgtagg aaaccccgat
     3601 aatctggaat ttgaaattac aaaaaaagca ggttataaat ttctgggagt taaaatttct
     3661 gctatgccaa gaaaaatagg ttttttgggg ctgaattttt taatttggtt ttttcagctt
     3721 caggcagcaa cttttaaggc acttttctac attaaaaaat acaaacccga cgcgattttt
     3781 ggcacaggcg gttatgtttc tgcgcctgca ctttttgcgg caaaccttgc agatgttcct
     3841 tttatgatac acgactgcga cgttaagccg ggtattttat caagatatat atcgcctatg
     3901 gcaaagaccg tttcacttgc ttttgaaggc gcaaaagaat acatgaaatc acaaaacatt
     3961 aatgtaaacg gaaatcctat cagagaaagt ttttttgagt cttcgaaaaa agaagcaaga
     4021 gaaaaactcg ggattcaaga taaaatgacg ctttgtatta tgggcggctc acaaggagct
     4081 aaatcaataa ataccgcagc ggtgggtgtt ttaaaatatt tatgtgaaca gcgtgatgta
     4141 caaataattt tccaaacggg aaagaaaaag tttgatgaag ttactgccct gctcaaagaa
     4201 atttatcccg aatttgaaag caataaaaat ttgatattaa aaccttacat tgacgacatg
     4261 gcatcagtgc ttaaagcaag cgatatagca gtttccagag caggctcttt gagcttgtct
     4321 gaactttgcg cctcaggggt tgcatcgatt ttaattcctt atccgcacgc tgctgctgat
     4381 caccaaagaa aaaatgccga ttttatggtc aaagaaagag cagcacttta ccttgaagat
     4441 gccgacacca caaaagacac ccttttgatt gcaatagagc agattttaga cagccaagaa
     4501 aaacttaaaa tcattcagga aaacgccttt gcacttgcaa aaccgaaggc gacaaaagat
     4561 attgtcgggc aacttaaagg aataattttt gagtaaacag aaatataata aagcaatagg
     4621 aattttaact tctcaaggca ggtttcacat aaaattaggg cttgagcgtg ttgaaaaaat
     4681 acttgaatat ttgggtaatc cgcaaaaaaa actcaaattt atccatgtcg cgggaaccaa
     4741 cggaaaaggc tctgtttgcg ccatgctaag cgcaatttta tcaaatattg aacccgcccc
     4801 ccttgagggg gagggacagg gaggggggta ttataaaata tcacgccttt attctaaaaa
     4861 aactctcgaa catgcaaaat gcttgcgaaa aaatatgact gatgcagaaa atattctttg
     4921 gtattattta agaaaaaaac agatgtgcgg ttttaaattt agaaaacaat cgccaattgg
     4981 aagttatatc gcagattttg tttgttttga aaaaaaactg ataatagagc tggatggcgg
     5041 acagcactta gaaaataatt ctattaaata tgatgaagta agaggaaatt ttttaaaaaa
     5101 agcagggttt gatgttttaa gattttataa tacagatatt tttaaaaata ttgaaagtgt
     5161 tttggagtgt atatttttat atttaaatcc ccccacccca aaccctcccc ctcaaggggg
     5221 gagggagcaa aactttaccc cccacaataa caattgcttt tcgattaatt acaataagca
     5281 agccttacgg tcctctgtgt taaaagtagg tttgtttaca agccctcatg tctttgaata
     5341 taccgaaaga attaaaatca atgacgctca aatcgacaaa aaaactttcg cgcaagaaat
     5401 aatcaaaatc accgagcttg cccaaaaaaa taatattgcc ctgaccgagt ttgaaatttt
     5461 aaccgccgtt gcttttgaat attttgccca aaataatgtt gatttggtta tccttgaaac
     5521 aggcttgggt ggaaggtttg atgccacaaa cataattaaa aaaaatattt gctcaataat
     5581 tacttcaata gattttgacc acaccgaaag actaggagat acgatagaaa aaatcgcttt
     5641 tgaaaaagca ggaataatta aatcaggcag ttttgttttg ataaataaaa ataacttggg
     5701 ttttgaggtg attaaaaaaa tcgcaaaaga aaaaaggacg caagttttaa ccgcagatgc
     5761 agggcggaat tttccgcagg aaaattccgc cctgcaaggc aattatcaaa aagaaaatct
     5821 ttcgcttgtg cttaaaacaa tagagttttt gaataaaaac ggatttaaaa tctgcaaaaa
     5881 agacattcaa aaaggtttgg aaaaatttaa gcatccctgc cggtttgaat ttcatacaga
     5941 aaatatttta attgatgccg cgcataatcc aagcggagct aaagcactaa gaaaaagtct
     6001 ggaaaaaaaa tttcctaatc aaaatttcag atttattttc ggatgcttaa gaaataaaaa
     6061 ttacccgcag atgatagaaa acttatttag ccccgaagat gaaatatttt tctatcaatt
     6121 cgacaaccca aattcttgtt cttttgagga gttaaatgcc gtttgtcctt ttgcggcaaa
     6181 atcttttaaa aaatttaata aatcggataa gataactgta atttgcggtt cgatttatat
     6241 gataaaagaa ttacttacaa aaattgataa aaagtagcat atcactaatt agtttgtgca
     6301 atgttttaat gaattgagga ttttattatg taggggtaaa tcctcaactc ttctgattgc
     6361 ttagtgctca cctctcaaat ttttgggagg ttttttttat taaaaaattg ccaccgtata
     6421 gcaaattatg atactataat ttgcgaaagg ctggaataat gaaaccctca aaaataattt
     6481 taataaccgt tttttctttt
//
LOCUS       DAOQAU010000072         6438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_14755_length_6438_cov_7.054990, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000072 DAOQAU010000000
VERSION     DAOQAU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14755_length_6438_cov_7.054990"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(737..1123)
                     /locus_tag="PKI94_08195"
     CDS             complement(737..1123)
                     /locus_tag="PKI94_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNW26752.1"
                     /translation="MNLNWYDTLNKSGLTPPSWVFAPAWTFLYITIIISFLLLIKDGN
                     LQAKALPITFFVIQMILNLIWSPLFFGKHLVLASLIDIILLWVFILLTIISFWQFSKT
                     ASLILIPYFLWVSFASYLNYKILILN"
     gene            complement(1131..2942)
                     /locus_tag="PKI94_08200"
     CDS             complement(1131..2942)
                     /locus_tag="PKI94_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNW26753.1"
                     /translation="MTEEKTTFKNIIQAMGLVFGDIGTSPIYTLTVIFLTTQINAQNI
                     LSILSLIIWTLLLIVTIQYTWLAMSLSQKGEGGTIVLNELLKKLLKKGRSVAFFSFLT
                     YVGISLLIGDSVITPAISILSAVEGLKFIHFLHNIPQSTILVITLIIAIALFSIQKKG
                     TENISKIFGPIMVIWFVALGLSGLFSILEAPLVLKAFNPIYGLEFLFKNGIAGFFVLS
                     EVILCATGAEALYADMGHLGRKPIVYAWYIVLFALLLNYLGQGAFLLKHPDVQNILFS
                     MIVHQARFLYIPFLLVSLMATIIASQAMISGLFAIIYQAINTRILPLFRVEYTSEKIN
                     SQIYIPSANWFLMFFVIFVILFFKESSKLASAYGFAVTGTFNITIILISIIFFLRKKY
                     FHFMLALMLMIIDMSFLIAAFSKIHTGAYWSIIIATIPFFLIILYVKGQKAMYQAINL
                     MDKEEFLQKYHERYSKGSKIEGQALFFARGIEKVPPYVVQTMFENSIMYSKNIFVHVN
                     KSSEAFGLHYEMETVAEGLQLLRIDAGYMQLFNVDSVLKALDIDGKAIFYGVEDIETN
                     NFFWHIFSTIKKITPSFVSFYNLPTNKMHGVVTRLKL"
     gene            complement(3058..4047)
                     /locus_tag="PKI94_08205"
     CDS             complement(3058..4047)
                     /locus_tag="PKI94_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNW26754.1"
                     /translation="MDLLIFLLLVIFFIFITGLCVGSFLNVVILRAFSNESIIFPPSK
                     CPACQKPLRWYHNIPVLSYLFLRGKCSFCDEKISLQYPIVEIVTAFLFVAVFMKFGFS
                     INLIFMLIFSSLFIVFAVTDIKEKVVFDFHTYTLAILGLIYNFFNFGHLYYGVKIINL
                     GQFALQINNSFIQAILGVLAGIIIMEALARFGYLIAGTRAFGEGDTFIAAGLGAVFGL
                     KYLITVLAYSLIIQVVLTIPVFFKKLYCQKDYQTLAAFIAFFAFAFGIKALDNYELLN
                     NLLVFGVLILILCVLGFYTCKRILQGLKNPENITYLPFGPAMIAGAFFVMFLL"
     gene            complement(4047..4826)
                     /locus_tag="PKI94_08210"
     CDS             complement(4047..4826)
                     /locus_tag="PKI94_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW26755.1"
                     /translation="MSQHEDTFVQIHEWLLTYRNSKDEKLKKQLKNLIVIAAMPFVKR
                     IACGLARRATDPVDDLIQVGSLGLVKAIDLYNPEISNKFKTYATYLITGEIKHYLRDK
                     ASMIKAPREIQELAYRINCVIKQLNEEGFDEPTSEQVAQIMSIPINKIEGIIEVDRRK
                     STLSLDQSTIYDDEDTALIDKIPSGDYQEFMDSYEKKIMLSGAIKKLEPNLKEIIELS
                     YYDELNQREIAQKLNISQMQVSRRLKRALNRMYNIITKAGK"
     gene            5079..5549
                     /gene="rfaE2"
                     /locus_tag="PKI94_08215"
     CDS             5079..5549
                     /gene="rfaE2"
                     /locus_tag="PKI94_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNW26756.1"
                     /translation="MGEVIKRESIKALVERLHKEGKTVVTTNGCFDILHVGHVRYLQK
                     TKSFADISIVLLNSDKSVRTIKGKGRPINSEKDRAEVLCALACVDYVVFFDENSPRDL
                     LAEIKPDVHTKGADYNEKNLPEADVILKNGGRIEFIDFVEGKSTTGLIEAMKKT"
     gene            5622..>6438
                     /locus_tag="PKI94_08220"
     CDS             5622..>6438
                     /locus_tag="PKI94_08220"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNW26757.1"
                     /translation="MKTYTIEEISQIVGGLLSKVQDAKISMLAPPLLADEKCLALALS
                     EEEIQNLAQTKAKAALVPLGVNFENISTIEVERPRLAMMKLLNLFYTEPEVQKGVHPS
                     TIVHPDAKLGQNVAIGPNVVVSRGVQIADGTKIVANSYIGSDVKIGKNCFFHPGVNIG
                     DRVKTGDNVILHHGVSLGADGFSFVTENPDNIEQARKDGAILEENISHKIFKIPSIGS
                     VQIGNNVEIGANTTIDRGTIEDTIIGDDTKIDDQVMIGHNCRVGKGCMIISQVG"
BASE COUNT         2225 a         1108 c         1171 g         1934 t
ORIGIN      
        1 tgcctcattt cttttctttt tttcttgcga ttgaaaaaaa gaaaagaaac caggcggaaa
       61 gaaaagaaaa aagaacgcga acaaaatgac aaagcgtcag tgcttcgcac tgaaaaggac
      121 tattcaattg atgaacaaac tcatcaattg aactaagggt ttcagagtga aaccctgtac
      181 ggactctttc ccgaccttat cggagtgagc aattattact taacgaaaca actccaaagc
      241 gagtgtgttt gagtgccaaa ggcacgagtt cgcgagcttg agttgagttt agtaagaatt
      301 agcgaacgca gatccggtcg ggagcttctt tgcctacttt cttgtcgaca caagaaagta
      361 ggtttataaa aaataattac aaaaattatt cgtttgtgag atcctgatac tattggagac
      421 aatagtctaa agcagggata attcaaacag gtcgtaagga cattttccac gaaatcaaag
      481 atttcgggaa aagaagtcaa tcaagttcag gatgacgtgg ggttggattg cttcttcaga
      541 aataaattac ctctcacaaa acctgacatt tagtcagttt ttgttcggca gagttcgcaa
      601 taacgtgagc gggtggacaa aaaatcaaaa tacatttacc cctcgcaatg acttgagtgg
      661 gcgaatttgg actgttagtc tttataacaa attttgtcaa aactgtgtat ttttaaatta
      721 ttccgtgttt atacaattaa ttcaaaatta aaattttata attcaaataa ctcgcaaagc
      781 ttacccacaa aaagtatggg attaaaatca aagaagcagt ttttgaaaat tgccaaaagc
      841 ttattatcgt aagcagtata aaaacccata ataaaattat atcaattaaa gaggcaagta
      901 ctaaatgttt gccgaaaaaa agcggcgacc agattaagtt caaaatcatc tggataacaa
      961 aaaaagttat cggtaaagct tttgcctgaa gatttccgtc ttttatcaac agtaaaaaag
     1021 aaataataat tgttatatac aaaaaagtcc atgccggtgc aaacacccac gaaggcggtg
     1081 tcaaaccgga tttgtttaaa gtatcgtacc aatttaaatt cataaaaacc tcaaagcttc
     1141 aatcttgtta ccacaccgtg cattttgttt gtcggaagat tatagaaact tacaaaagac
     1201 ggcgttattt ttttgatggt tgagaaaata tgccagaaga aattgtttgt ttcaatgtct
     1261 tcaactccgt aaaaaatggc tttaccgtca atatccaagg cttttaaaac actgtcaaca
     1321 ttaaaaagct gcatgtaacc ggcgtctatt ctcaaaagct gaagcccctc tgcgaccgtt
     1381 tccatttcgt agtgaagccc gaatgcttcg cttgatttgt ttacgtgtac aaaaatattt
     1441 ttactgtaca ttatgctgtt ttcaaacatt gtctgaacca cataaggcgg aactttttca
     1501 atgcctcttg caaaaaacag tgcctgacct tcgattttgc tgcctttgga gtatcgttca
     1561 tgatattttt gtaaaaattc ttctttgtcc ataaggttaa tagcttgata catggctttt
     1621 tgccctttaa cataaagaat tataaggaag aaaggaattg tcgcgataat tattgaccag
     1681 taggctcccg tgtgaatttt ggaaaaagcg gcaataagaa aagacatgtc gataatcata
     1741 agcattaagg caagcataaa atgaaaatat ttttttctta aaaagaaaat gatacttatt
     1801 aatattattg ttatattgaa agttccggtt acagcaaagc cgtaagctga ggcaagtttt
     1861 gacgattctt tgaaaaacag aattacaaaa attacaaaaa acattaaaaa ccagtttgca
     1921 cttggaatat aaatttggga atttattttc tctgaagtgt actcaactct gaaaagcggt
     1981 aaaattcttg tgtttattgc ttgataaata atggcaaaaa gtccgcttat cattgcctgt
     2041 gaggctataa ttgttgccat taagcttact agcaagaaag gtatgtataa aaatcttgcc
     2101 tgatgcacaa tcattgaaaa taaaatattt tgaacatcgg ggtgtttaag caaaaatgca
     2161 ccttgcccca gatagttcaa aagtagcgca aaaagtacta tataccaagc ataaacaatc
     2221 ggttttctgc ccaaatgtcc catatccgca tatagggctt ctgctccggt tgcacaaaga
     2281 atgacctccg acaaaacaaa aaatcctgct attccgtttt taaaaagaaa ttccagcccg
     2341 tatatagggt tgaaagcttt cagtacaagc ggagcttcaa ggatgctgaa taatcccgac
     2401 aaaccgagtg ccacaaacca aatcaccatt ataggaccga atatttttga aatattttct
     2461 gttccttttt tttggattga aaaaagggca atagcaatta ttaaagttat aaccaaaatt
     2521 gtggattgag gaatattatg caaaaagtgg ataaatttta aaccctcgac tgcagataaa
     2581 atgcttatgg caggagttat aacactgtcg cctatcaaaa gcgaaattcc cacataagtt
     2641 aaaaatgaaa aaaatgcgac cgaacgacct tttttaagca acttttttaa taactcatta
     2701 agaactatcg taccgccctc gcctttttgg ctcaagctca ttgcaagcca agtgtactga
     2761 atagttacaa tcaacaaaag tgtccaaatt attaaagata agatgcttaa tatgttttgg
     2821 gcatttattt gagttgtcaa aaatattaca gtaagtgtat aaatagggct tgttccgata
     2881 tctccgaaaa ccagacccat tgcttgtatt atattcttaa aagttgtttt ttcttctgtc
     2941 atgttattct cctgccaaca gattttactc tattacatac aaaatccaaa atcaaggggg
     3001 tgctgtgcac caagacactc tgtatagatt tttttcaaag agtttcaaca ggtcacttta
     3061 aagtaaaaac attacaaaaa acgcacctgc aatcatagca ggaccgaaag gtaaataagt
     3121 tatattctct gggtttttaa gtccttgaag aattcttttg caagtataaa atccaagcac
     3181 gcaaagaatt aaaatcaata ctccaaaaac taaaagattg ttaagcaatt cataattatc
     3241 aagagcctta atcccgaagg caaaagcaaa gaaggcaata aaagcagcaa gtgtctggta
     3301 atctttttgg caataaagtt ttttgaaaaa gacggggatt gtaagcacaa cttggataat
     3361 caaactataa gcaagaactg taattaaata tttaagcccg aaaaccgcac ccaagccggc
     3421 ggctataaaa gtgtcgcctt cgccgaaggc tcttgttcct gcaataagat agccgaacct
     3481 tgcaagagct tccattatta ttattcctgc aagaacgcct aaaatagctt gaataaaaga
     3541 attatttatt tgaagcgcaa attgacctaa atttattatt ttaaccccgt aatacaagtg
     3601 tccgaagtta aaaaaattat aaataagccc caaaattgca agagtataag tgtgaaaatc
     3661 aaaaaccact ttttctttta tatcggttac tgcaaaaaca ataaaaagag agctaaaaat
     3721 aagcataaaa ataagattta tgctaaatcc gaatttcata aaaacggcaa caaacaaaaa
     3781 agcggttaca atttctacaa tcggatactg aaggcttatt ttttcatcac agaaagagca
     3841 tttgcccctc aaaaacagat aagataaaac gggaatatta tggtaccacc taagcggttt
     3901 ttggcaagca ggacattttg aaggggggaa aatgatggat tcattgctga aggctcttaa
     3961 aattacaaca tttaagaagc ttcccacgca aagtcctgtt ataaaaataa aaaatattac
     4021 aagtaataaa aatattaaca aatccattat ttacccgcct tggtgattat attgtacata
     4081 cggtttaagg cccttttaag tcttcttgaa acctgcattt gagagatatt cagtttttgt
     4141 gcaatttctc tttggttcaa ttcatcataa taactcaatt ctattatttc tttcaaatta
     4201 ggctcaagtt tttttattgc tcccgagagc attattttct tttcgtaaga atccataaat
     4261 tcctggtaat ctcccgatgg aattttgtct ataagtgcag tgtcttcatc atcataaatt
     4321 gttgattggt caagcgagag agtgcttttt cttctgtcaa cttcaataat tccttcaatt
     4381 ttattaatcg gaattgacat gatttgtgca acttgttcag aggtgggctc gtcaaatcct
     4441 tcttcgttaa gctgttttat cacgcagtta atcctgtatg caagttcttg aatttcacgc
     4501 ggggctttaa tcatagaagc tttatccctt aagtaatgct taatttcgcc tgttataaga
     4561 tatgtagcat aagttttgaa cttgttgctt atttcagggt tgtataaatc tatagctttc
     4621 acaagcccca gtgaacctac ctgaataagg tcatccacag ggtcggttgc acggcgggca
     4681 agtccgcatg cgattctttt tacaaaaggc atagcggcaa ttacaattaa gttttttaac
     4741 tgttttttta acttttcatc ctttgaattc ctgtaggtta acagccattc gtgaatttgg
     4801 acaaaagtat cctcgtgttg tgacaaaaaa atttcctctc tgattgaaat aatcttatca
     4861 ttatttttta aaatttacaa taatataaaa gttgaaaagt cgaacagtcg aatagtttat
     4921 aagaagataa gatgagtaga tgcgtggatg cgtgggttat cctactacgc ctcttggtat
     4981 cttcgcctct tgtcccctgt tttaacattt ctattcacta tttactgttt actcttttta
     5041 tgctaaattt gacttatggt ttttaaaaga gggcataaat gggcgaggta ataaaaagag
     5101 agagcataaa agcacttgta gaaagacttc acaaagaggg caaaactgtt gttaccacaa
     5161 acggctgttt tgatattttg catgtaggtc atgtaagata tcttcaaaaa accaaaagct
     5221 ttgcagatat ttcgattgtg cttttaaatt cggataaatc cgtccgtacg ataaagggta
     5281 aaggacgccc gattaattcc gaaaaggaca gggcagaggt gctttgcgct ttagcttgtg
     5341 ttgattatgt cgtgtttttt gatgaaaatt caccgagaga tttgctcgct gaaatcaaac
     5401 ccgatgtgca cacaaagggc gcagattaca atgagaaaaa tcttcccgaa gccgacgtta
     5461 ttttaaaaaa tggcggcaga atcgaattta tagattttgt tgagggaaaa tccacaacag
     5521 gtttgattga agcaatgaaa aaaacttaat aatttgataa atattcaaat taatattaaa
     5581 atcaatatta atatttgagg aaattataac aggagaagaa gatgaaaact tatacgattg
     5641 aagaaatatc acaaatagta ggcggactgt tgagcaaagt tcaagatgca aaaatttcaa
     5701 tgcttgcgcc tccgctttta gctgatgaaa aatgtcttgc gctggcactg agcgaagaag
     5761 agatacaaaa tcttgcccaa acaaaagcaa aagcagcact tgttccgctt ggggtgaatt
     5821 ttgaaaatat aagtactata gaagtcgaaa ggccacgcct tgcaatgatg aaattgctta
     5881 atctttttta cacagagcca gaagttcaaa aaggtgttca tcccagcaca atcgttcatc
     5941 ctgatgccaa attgggtcaa aatgttgcga taggtcctaa tgttgtagtt tcaaggggcg
     6001 ttcaaattgc agacggaaca aagattgtgg caaattctta tatcggaagt gatgtaaaaa
     6061 tcggtaaaaa ctgctttttt cacccgggtg taaatatcgg cgatagagtt aaaaccggcg
     6121 ataatgtaat tttacaccac ggagtctcat tgggcgcaga cggtttcagc tttgtaacgg
     6181 aaaaccccga taatatcgaa caggcaagaa aagacggggc tattttggaa gaaaatattt
     6241 cccacaaaat ttttaagatt ccttcaatag gttctgttca aataggtaac aatgtcgaaa
     6301 tcggcgcaaa cacaactatt gacagaggaa ccattgaaga tacgattatc ggtgatgaca
     6361 caaaaattga tgatcaggtc atgataggtc ataattgcag agtaggcaaa ggctgtatga
     6421 ttatttctca agtcggta
//
LOCUS       DAOQAU010000073         6155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_15775_length_6155_cov_6.569836, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000073 DAOQAU010000000
VERSION     DAOQAU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15775_length_6155_cov_6.569836"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PKI94_08225"
     CDS             complement(<1..671)
                     /locus_tag="PKI94_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26758.1"
                     /translation="MIKDFLSKLGASTKTTVGVSISPGIGLEMVEVDKNTKTVLKYGH
                     KPLEYNHSNRDIEDYAAFQEALGGLFDELHIPRRSNIVITIPNINFGIINLPILLTDE
                     AVNNAIISEVEQSYIFKRFEPAISWREIGSNIDTERRTLAYTAIQKTAIEGFKAACKE
                     VGCTLLAIETSLASIFKTLSFSDLAADEMKDNISWNLMVVGQNSYSVFGMFGKKIMEY
                     YEEPLA"
     gene            760..1665
                     /gene="nadA"
                     /locus_tag="PKI94_08230"
     CDS             760..1665
                     /gene="nadA"
                     /locus_tag="PKI94_08230"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNW26759.1"
                     /translation="MKMIEEILKLKKEKNAVILAHCYQNIEIDEVADFIGDSLYLSQQ
                     AAKTEADIIVFAGVYFMAETAKILSPDKKILLPRLESGCQMANMINLAQLREFKFKNP
                     NIPTVCYVNSTAEVKSECDICCTSSNAVKIVSSLGVKEVLFVPDTYLGKWVESKLEGV
                     KVITFPGHCPTHLRITVKDILQQKSQYQNALVLAHPECHQSVVEIADYVGSTTGIMKF
                     AAQSDKKEFIIATEIGVVERLERDYPDKKFILASPKAICHNMKWHHLEDIYNALKYEQ
                     YEIEVNEQISKKAVNCINRMLEVSK"
     gene            1726..2172
                     /locus_tag="PKI94_08235"
     CDS             1726..2172
                     /locus_tag="PKI94_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26760.1"
                     /translation="MELIKILLDLTLAICAVIALKSWKKEIKAKKKKELADKLFECIG
                     YIEYLLIPSDEMAYPSAIYYKTLEKFGISCQENKQRLQKIWIDLKDLNQEYQQLKNID
                     IATKISKIEDFIQSKFKDESIDNNSLKEKIEYIKKFCIEENKKFYS"
     gene            2665..3441
                     /gene="rlmB"
                     /locus_tag="PKI94_08240"
     CDS             2665..3441
                     /gene="rlmB"
                     /locus_tag="PKI94_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNW26761.1"
                     /translation="MENQQSEIIYGKNSVLEALISGKREINKILIANNIHSDAKLNEI
                     KKLAQQKGIVFQFVAKEKLGVNVGVNEAHQGVMALISPIKYTDLDEFLEQKHENASLI
                     ILDGIEDSHNLGAIIRTCVCAGVSAIIIPSRRNVQVNSTVEKTSAGAVNHIPIIKVNS
                     LISAVQKLKNNDWWVIATEASAKDNYYNIDYTGMNFALVMGAEHAGVSRAIIKEADFL
                     VKIPMQRDFNSLNVSNALSAIIFETLRQKNEKCSIINPKS"
     gene            3605..3843
                     /locus_tag="PKI94_08245"
                     /pseudo
     CDS             3605..3843
                     /locus_tag="PKI94_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     assembly_gap    3844..3943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4155..5843)
                     /gene="glgP"
                     /locus_tag="PKI94_08250"
     CDS             complement(4155..5843)
                     /gene="glgP"
                     /locus_tag="PKI94_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNW26762.1"
                     /translation="MALPKVAYFSMEIAMDQSLSTYSGGLGFLAGSHMQSAGYLQLPM
                     AGVTMLWSYGYYDQRIDHNGEVEVAYIRKYYDFLTDLDVYTDVDVFGEKVKVKAYRVE
                     PELFGTCPVYLLTTDIEGNSEWARSISHKLYDGDEKIRIAQETILGIGGIRILQQVGY
                     NFDVVHMNEGHALPAAFELLRQYNGDLERVKQTTVFTTHTPVAAGNEVHWVDTLMEAG
                     FFAGCPRDRVIALGGENFSLTVAALRMSRIANAVSQLHGLVANKMWEWVDGRCPIRAI
                     TNAVNLHYWQDHRIANSKNHKDLLKAKRDMKVELFKYIADTAGKRFDPDVLTITWARR
                     FADYKRAWLILMDKDRLNKLLDENKIQIIFAGKFHPSDVMGREMFNKILHRSHSLKNV
                     VVLPGYELELSAKLKRGSDIWLNTPLRPFEASGTSGMSANMNGALHLSIYDGWTVEGT
                     FNGINGYSVEYPGLDDEIPWEERHWKDHDYIMNIIENEIIPTYYENKEEWARLMRQAI
                     RTSEAYFNSDRMVIEYYSRLYKSIAHDDTSSENKKDIRVADSPTFDAWTFSNIK"
BASE COUNT         2074 a         1098 c         1072 g         1811 t
ORIGIN      
        1 gctaagggct cttcataata ttccattatc tttttgccga acatcccaaa aacagaataa
       61 ctattttgcc ctacgaccat aagattccag cttatattat ctttcatctc atcggcggct
      121 aaatccgaaa aagataaagt tttaaaaatt gaagcaagag aggtttctat tgccaaaagc
      181 gtacatccga cttctttaca agcagcctta aacccttcaa tggcagtttt ttggattgct
      241 gtataggcaa gggttcttct ttcggtatct atatttgagc ctatttcccg ccagcttatt
      301 gcaggctcaa atcttttgaa gatataagac tgttcaactt cggagataat agcattattc
      361 acagcttcat cagtcaacaa aatcggaagg tttattattc caaaatttat gtttggaatt
      421 gttatcacta tattgcttct tcttggaata tgaagttcat caaacagtcc gcccaaagct
      481 tcttgaaaag cggcataatc ttctatatcc ctgttggagt gattatactc aagaggcttg
      541 tgtccatact taaggactgt ttttgtgttt ttatccacct caaccatttc caagccgatt
      601 ccgggtgaaa ttgaaactcc caccgttgtc ttggtgctgg ctcccaattt tgataaaaag
      661 tctttaatca taaaccttaa atttgttacc taatctcatt tttattatac aatatatttt
      721 gtaataagca tattttaaac ataactttac atcttttgaa tgaaaatgat agaagaaata
      781 ttaaaactta aaaaagaaaa aaatgcggta attttagcgc attgttatca aaacatcgaa
      841 attgacgaag ttgccgattt tatcggtgat tccttgtatt taagccaaca ggctgcaaaa
      901 acagaagctg atattatcgt ttttgcgggg gtttatttta tggcagaaac cgcaaaaatt
      961 ttgtctccgg acaaaaaaat tctgcttccg cgtcttgaat caggctgtca gatggcaaat
     1021 atgataaatt tggcgcagtt aagagaattt aagtttaaaa atcctaatat accgacagta
     1081 tgttatgtaa attcaactgc cgaagtcaaa tccgaatgcg acatttgctg cacaagttca
     1141 aacgcggtta aaattgtcag cagccttggt gtaaaagaag ttttgtttgt tcccgacact
     1201 tacttgggca aatgggttga aagcaaatta gaaggagtga aagttataac cttccccgga
     1261 cattgcccca cgcatttaag gattacggtt aaggatattt tgcagcaaaa aagccaatat
     1321 caaaatgccc ttgtgcttgc gcatcccgaa tgtcatcaat cggttgttga aattgcagat
     1381 tatgtcggaa gcacaacggg tattatgaaa tttgccgcgc aaagcgataa aaaagaattt
     1441 attattgcaa cagaaatagg cgttgtggaa cgcttggaaa gagattatcc ggataaaaag
     1501 tttattcttg cctcgcccaa ggcaatttgc cacaatatga aatggcatca tttggaagac
     1561 atttataatg ctcttaaata tgaacaatat gaaattgaag ttaatgaaca gatttcaaaa
     1621 aaagcagtta attgcattaa caggatgtta gaggttagta agtaataaat attttcgtcg
     1681 ttgcgaggaa cgaagcgacg cggcaatcca aaaataggag ataaaatgga attaataaaa
     1741 atacttttag atttaacttt ggcaatttgt gccgttatag ccttaaaaag ttggaaaaaa
     1801 gagataaagg cgaaaaagaa aaaagaactg gccgataaac ttttcgaatg tattggatat
     1861 attgaatatt tacttattcc aagcgatgaa atggcttacc ctagtgcaat ttattataag
     1921 acattggaaa aatttggcat atcttgccaa gaaaataaac aaaggcttca aaagatatgg
     1981 attgatttaa aggatttaaa tcaggaatat caacagttga aaaacattga tattgcaaca
     2041 aaaattagta aaatagaaga ttttatccaa agcaaattta aggatgaatc tatagataat
     2101 aatagtttaa aagaaaaaat tgagtatata aaaaaattct gtatagaaga aaataaaaaa
     2161 ttttattctt aataattaat cttccaaata aaatttaaaa attgaaatta acgagtaact
     2221 ttttgtttgt tcatttcttt tcttgttttt gcgattaaac aagaaaagaa acagaaccta
     2281 gtcactattc gtgacgccat ttcgcacgaa aaacaagttt tcggcgaaag aaggcaagaa
     2341 aagaaaaaaa cgcgaacaaa atgccaaagc gtcaggggca agcccccgaa cccccattta
     2401 attattttga ctttatcgaa gtgagcagaa taaaagggcc ccctttagcg agttctttta
     2461 gtttgagtag gcgaaatgta atgcccgaaa atccgattgt ttgagcatag cgagttgagg
     2521 attttcaatg taatgagccg tactcaaatt taaaaacgag tagcaggggg tggctttttg
     2581 cggtactttt tgccatcaaa aagtacctaa agaaaaatat aagaaagaaa aaataacaaa
     2641 acatgtttaa tgtataatca ttttatggaa aatcaacaat cagaaatcat ctacggaaaa
     2701 aattccgtcc tggaagcttt gatttcaggc aaaagagaaa tcaacaaaat tttgattgca
     2761 aacaatatcc acagcgacgc taaattaaac gaaataaaaa aactcgccca acaaaaagga
     2821 attgtttttc agtttgtggc aaaagaaaaa ttgggggtaa atgtaggggt aaatgaggcg
     2881 catcaaggtg taatggcttt aatttcaccg ataaaataca ccgatttgga tgaatttctt
     2941 gaacaaaagc acgaaaacgc ttcgctgata attcttgacg ggatagaaga ctcacacaat
     3001 ctcggtgcga taataagaac ctgtgtctgc gccggagtaa gcgcaataat aattccttca
     3061 aggcgtaatg ttcaggttaa ttccactgtt gaaaaaacct ccgccggtgc tgtaaatcac
     3121 attccgataa taaaagtaaa tagcctgata agtgctgttc aaaaattaaa aaataacgac
     3181 tggtgggtaa ttgccacaga ggcgtctgcc aaagataatt attataacat cgattacacg
     3241 ggcatgaatt ttgcacttgt aatgggagca gagcatgcag gagtttcaag agcaataatc
     3301 aaagaagcgg attttctggt taaaatcccg atgcaaagag attttaactc gcttaatgta
     3361 tcaaatgcac tcagcgcaat aatttttgaa acacttcgac aaaaaaacga aaaatgtagt
     3421 ataataaatc caaagagtta acggggtttt tgcaatgcct aacgaaaaag aacaattaat
     3481 agacattgaa gataatgttt cggaagcaga atctgtggat ttttctaaac tggaagagga
     3541 agaagacatt cttgatactg ttgaagaacc gaaaattcag gaaaatttaa cctcaataaa
     3601 cacagatgat tccgttcgta tttatcttca gcagataggc aaaattccat tactttcttc
     3661 tgaggaagaa cttcaaatcg caagaaaaat taaagaagaa cattgtgaaa tgtctaaaaa
     3721 cattcttgtt aatgccaatt tacgtcttgt agtaagtatc gccaaaaaat atataggtcg
     3781 cggactttct tttcttgact tgattcagga aggaaatatg ggactgatga aagcagccga
     3841 aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacaata acggaaccaa
     3961 aaaaacgctt gatgaaatcg gctctcaata cggggtttca agagaaagaa taagacaaat
     4021 cgaaaacaga gccatttcaa aacttaaaaa actctgcaaa aacacaaacc ttacaaaagg
     4081 attgaaaaat tacttcggag ggtaaaatac cctgaaattt taatccgtta tcaaaacttt
     4141 atttttagct ttatttactt aatatttgag aaggtccatg catcgaaagt aggactgtct
     4201 gcgactctta tatctttttt gttttctgaa gaagtatcat cgtgagcgat tgatttgtaa
     4261 agtctgctgt aatattcaat taccattcta tcagaattga agtaagcttc tgaagtcctt
     4321 atagcctgac gcatgagtct tgcccactct tccttgtttt catagtaagt aggaataatc
     4381 tcattttcga ttatattcat aatataatcg tgatctttcc agtgtctttc ttcccaagga
     4441 atttcatcat caagtcccgg atactcaacc gaatatccgt taattccgtt aaacgtacct
     4501 tcgaccgtcc agccgtcata aattgaaagg tgcaaagctc cgttcatatt agcggacatg
     4561 ccggaagttc ctgaggcttc aaaaggtctc aaaggagtat ttagccaaat atcagagcct
     4621 cttttgagtt ttgcgctcaa ttcgagttca taacctggca gaacaacaac atttttaagc
     4681 gaatgtgagc ggtgaaggat tttgttaaac atttcacgtc ccataacatc gctggggtgg
     4741 aatttacccg caaaaataat ttgaatttta ttttcatcca aaagtttgtt taatctgtct
     4801 ttgtccatta aaatcagcca tgctctttta taatccgcaa atcttcttgc ccatgttatt
     4861 gtcaaaacat cggggtcaaa tcttttaccg gcagtatctg caatgtattt aaagagttca
     4921 actttcatat cacgctttgc tttgagcaaa tctttatgat ttttggaatt agcaatcctg
     4981 tggtcttgcc agtaatgaag gtttacggca ttggtaatcg cccttatagg acatcttccg
     5041 tcaacccatt cccacatttt gtttgcaaca agcccgtgaa gctgagaaac agcatttgca
     5101 atcctgctca tcctcaacgc tgcaacggtt aacgaaaagt tttcaccgcc aagtgctata
     5161 actctgtcgc gaggacagcc tgcaaaaaat cctgcctcca taagcgtatc gacccagtga
     5221 acttcgttac ctgcagcaac aggagtgtga gttgtaaata cggttgtctg cttaaccctt
     5281 tccaaatcac cgttatattg tcttaaaagc tcaaatgccg caggaagtgc gtgaccttcg
     5341 ttcatatgaa caacatcaaa attatatccg acttgctgaa gaattcttat accgcctatt
     5401 cctaaaatgg tttcttgggc gattcttatt ttttcatctc cgtcataaag cttatgggaa
     5461 atgcttcttg cccattcact gtttccttca atatctgttg taagaagata aacaggacat
     5521 gttccaaaca attcaggctc aaccctgtaa gctttaactt tgactttctc gccaaaaaca
     5581 tctacatctg tataaacatc caaatcggtc aaaaagtcat aatactttct tatataagca
     5641 acttccactt ccccgttatg gtcaattctc tggtcataat aaccgtaaga ccaaagcatt
     5701 gtaactcctg ccatgggaag ctgcagataa cctgcagact gcatgtgtga acctgctaaa
     5761 aaccctaacc ctcccgaata tgtgctaagc gactgatcca tcgcaatttc cattgaaaaa
     5821 taagctactt ttggtaatgc cataaattta acaccttttc tattttacta acactttata
     5881 cttaaaaaat tttttttcaa ttagtcctat gtctttatta ataaacaata taacacattt
     5941 aaaaaaaatt gtttatttat ttttcaaaaa ccctccaaat aatcgaaacc caatatctaa
     6001 aagatttttt tattaagttt tttgaagaaa ggccgctgca caagtataga aaatttgttt
     6061 tttctcactt gtgcagcggc cgaccttaca aaagcgagag tggcaaaatc ggcacgagga
     6121 gtgccgttta gccattcgac actagccgtc tttaa
//
LOCUS       DAOQAU010000074         5813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_17141_length_5813_cov_7.189476, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000074 DAOQAU010000000
VERSION     DAOQAU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17141_length_5813_cov_7.189476"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKI94_08255"
     CDS             <1..552
                     /locus_tag="PKI94_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNW26763.1"
                     /translation="EFDEYPRALDEIVIKTEPRGVNKIIASRDFEIWNSQGSRLGRAA
                     SNWTLVDLNTKSILPIAKVIPFMIHFEKRESDMNFTKIMPPEKPEFEKTFEIRFDDID
                     VNRHVNNANYIVWAFEALPYDFKAQYRLKILDIAYKKEIAFGHKVVSCVQISEENGQK
                     VSLHAVKNATTQEELCLVKALWI"
     gene            complement(738..1130)
                     /locus_tag="PKI94_08260"
     CDS             complement(738..1130)
                     /locus_tag="PKI94_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26764.1"
                     /translation="MELSVPTALDNLAANGVINYDANAYIRGEKPKYGINPQMKAPGL
                     KMDSNAKMQEQPQKDEFKNSIEKIPSWKKILTGVLLVGTAVLLGKKIWKPKNINLKKS
                     SNVVRKPLKDFIAKPISWIKSKIKIKKP"
     gene            1375..2970
                     /locus_tag="PKI94_08265"
     CDS             1375..2970
                     /locus_tag="PKI94_08265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNW26765.1"
                     /translation="MLWILLGVGIFLTYAHHGHLIIDCGREVYYPTQILEGKLLYKDL
                     FNIYGPFSYLFNAVLFKLFGINLNVLYLAGCFCAFLITTLIYKIARKFLTEIISFSIA
                     FFTICTGVLNTNLFNFIFPYSYAMLYGMVAFLISFLFLAKYIQEPHKVLNLYLSGFFA
                     GFCIANKYEFLPYLLVFLYMVLKIEKVSFKTILLLLLSILSIPLVCFSILFLQGMGIN
                     DLVLAFETVKKMAQSQTLKYFYTTQGVVLDKKTLGYLALNFAKTVTPILLMTWSFMMS
                     KKWLSVLLIIFATASGMIFMSPASLTFLPVLILGLFILHFRKYKNEKIVLILALSAIL
                     ISLKSFWGLATLNYGVFFAGFLIVAIFALIENKFYQKAIFVYIILLSLVLGFQNLSEL
                     KDKSYCIQTSRGKICSQKYLTKSSEDLINFIDKNTKKTDSMVILPEGLVINFLMERKS
                     DDYYNSLIPLYWEVFGDEKLIEYFEKTKPDYIVFSNWDNKDYYFRYICSDYAVSFCNY
                     VAKNYRQEKIIDNGFRYLIYRKK"
     gene            complement(2975..3233)
                     /gene="ffs"
                     /locus_tag="PKI94_08270"
     ncRNA           complement(2975..3233)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI94_08270"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(3333..3470)
                     /locus_tag="PKI94_08275"
     CDS             complement(3333..3470)
                     /locus_tag="PKI94_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNW26766.1"
                     /translation="MARYVCTVCQWVYDEEKEGTKFEDLAADWTCPICGVEKDLFELQ
                     S"
     gene            complement(3499..4038)
                     /locus_tag="PKI94_08280"
     CDS             complement(3499..4038)
                     /locus_tag="PKI94_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNW26767.1"
                     /translation="MELKGSKTEKNLEAAFAGESMARNKYTYYAAKAKKEGYVQISHI
                     FEETANNEKEHAKIWYKLLHGGEVANTLTNLQDAANGENYEWTQMYPTFAKEAREEGF
                     EKIAYLFESVAKIEKDHNERYQKLHDNIKNEEVFEKKEKVFWICLNCGHILESTKAVE
                     VCPVCAHPKSYFSMRKIDY"
     gene            4120..4548
                     /locus_tag="PKI94_08285"
     CDS             4120..4548
                     /locus_tag="PKI94_08285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNW26768.1"
                     /translation="MKNILEQKVYYSDTDAYGVVWHGSYLRWLEMGRVELCEDMGYNL
                     IELENQDIVMPVVNINVRYKASAKINNELIITTKVKEFNSICVKFEQKITDKNSGKIF
                     IEALVEVVTVNREGKLYRKMPQILTEAFEKAMKDEEYALK"
     gene            4526..5260
                     /locus_tag="PKI94_08290"
     CDS             4526..5260
                     /locus_tag="PKI94_08290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNW26769.1"
                     /translation="MKNMRLNKYIAASGICSRRKADELIQSGVVSVNGKKVVELGFSV
                     NEKDKVFVKNELIRPKKFEYYRFYKPTGYITTSDDEKDRKIIYDLLPPAMHNLKPVGR
                     LDRDSSGLIILTNDGDLINELTHPSIKVPKVYVVSVEGKVSLHHLEKMAQGIEIEKGK
                     NAYADTLILESTNKKTILQVTLYQGLNRQIRKMFENLGFEVTSLKRIQHATITLEGLK
                     KGQFKPIKPLQVKELRNFLSKLEQKK"
     gene            5257..>5813
                     /gene="coaE"
                     /locus_tag="PKI94_08295"
     CDS             5257..>5813
                     /gene="coaE"
                     /locus_tag="PKI94_08295"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNW26770.1"
                     /translation="MIKVAITGNIASGKTAVEEILRKKGYQVLDTDDISHTLLEDKKV
                     KEQIISEFEKFDIVENNEISRHKLGKIVFTDIKLRKKLEEILHPLIKKEIRRFFRQCE
                     SKGEKIAFVSVPLLFEVNMQKLFDKIVLVHADTQFRLQRLVSRNNLTIEQAKNRIKIQ
                     MPQDEKLKLSDYVIYNNKTSKELELA"
BASE COUNT         1833 a          897 c         1051 g         2032 t
ORIGIN      
        1 gaatttgacg agtatccccg tgcgttagat gaaatagtaa ttaagaccga gcccagaggc
       61 gttaataaaa ttatcgcaag ccgtgatttt gagatttgga atagtcaggg tagcaggctt
      121 gggcgtgcgg caagcaactg gacactggtg gatttgaata caaaaagtat tcttcccatc
      181 gctaaagtta ttccctttat gattcacttt gaaaaaagag aatcggacat gaattttaca
      241 aaaataatgc cgccggaaaa accggagttt gaaaaaactt ttgaaataag atttgacgat
      301 attgatgtta atcgtcacgt taataacgca aattacattg tttgggcgtt tgaagctctg
      361 ccttatgatt ttaaagccca atataggtta aaaattcttg atatagctta caaaaaagaa
      421 atcgctttcg gacataaggt tgtttcttgc gttcaaatat cagaagaaaa cggtcaaaaa
      481 gtttcgctcc atgcggttaa aaatgccaca acacaagaag aactttgttt ggtaaaagca
      541 ctttggattt gataaaattt aagggcggat ttccttttta aggaaatccg cccctttttt
      601 tgcctttcac acaaaattga gaccactgca aaagtgcaga aaatttgttt tttctcactt
      661 ttgcagtggt tgaccttaca aaagtgagaa tggctcggta ctaggagtgc cgtttagcca
      721 ttcgacacta gccgtcttta aggctttttt attttgattt tacttttaat ccatgaaatc
      781 ggttttgcga taaagtcttt caagggtttt ctaacgacat ttgaactttt tttaagatta
      841 atattttttg gtttccagat ttttttgccc aacaggactg ctgttccaac taaaagaacg
      901 cctgtaagaa tttttttcca gcttgggatt ttttctattg aatttttgaa ttcatctttt
      961 tgcggttgtt cttgcatttt ggcattggaa tccattttta atcccggggc tttcatctgt
     1021 gggttaatgc cgtatttcgg tttttcacct cttatgtagg catttgcatc ataatttatt
     1081 acaccgtttg ccgctaaatt atctaatgct gtaggtacgc ttaattccat agtaatccaa
     1141 ccttttttaa tacacactta tataaagttt ttttttgtga aaaaattgct ttttaagtgt
     1201 tattttgtta aattctagta atatatgaaa aaattaagtg aattaatgtc attgcgagcg
     1261 aagcgaagca atccaaacaa gtcttttgtt tcttggattg cttcgtcact cggtttactc
     1321 ctcgctcctc gcaatgacag tggaaaattt agtttagatt taaaatattt ggttttgctg
     1381 tggattttgc ttggagtcgg aattttttta acttacgctc accacgggca tttgattata
     1441 gattgcggaa gagaagttta ttatccgaca caaattttag aaggaaaact cctctacaaa
     1501 gaccttttca acatttatgg acctttttct tacttattta atgccgtttt gtttaagctt
     1561 ttcgggataa acttgaacgt tttgtatttg gcaggctgtt tttgcgcttt tttaataacc
     1621 actttaattt ataaaattgc aaggaaattt ttgactgaaa ttataagttt ttcgattgct
     1681 tttttcacta tttgtacagg agttttaaac accaatcttt ttaattttat ttttccttat
     1741 tcttatgcaa tgttgtatgg aatggtcgct tttttaatat cttttttgtt tttggctaag
     1801 tatattcaag agccgcacaa agtcttaaat ctttatttga gcggattttt tgcggggttt
     1861 tgcattgcaa acaaatatga atttttgcct tatttgttgg tgtttttgta tatggtgctt
     1921 aaaatcgaaa aagtttcttt caaaacaatt ttgcttttat tgttatcgat tttgtcgata
     1981 cctttggttt gtttttcaat tttatttttg caggggatgg ggataaatga tttggtctta
     2041 gcttttgaaa cggttaaaaa aatggcacaa agtcaaacgc tgaaatattt ttatacaact
     2101 caaggtgttg ttcttgacaa aaaaacattg gggtatttgg ctcttaattt tgcaaaaaca
     2161 gtaactccga tattattaat gacatggagt tttatgatga gcaaaaagtg gctgtctgta
     2221 cttttgatta tctttgcgac ggcatcgggt atgattttta tgagtcctgc aagtttaact
     2281 tttttgcctg ttttgatttt gggattgttt attttgcatt tcagaaagta taaaaacgaa
     2341 aaaatagtct tgattttggc actaagtgca attttaatta gccttaaatc tttttggggt
     2401 ttggcgacat tgaattacgg ggtgtttttt gcaggatttt tgattgttgc gatttttgct
     2461 ttgattgaaa ataaattcta tcaaaaagca atttttgttt atattattct attatcgctt
     2521 gttttagggt ttcaaaattt gtcggaacta aaggataaaa gttattgtat tcaaacttca
     2581 agagggaaaa tctgcagcca aaagtattta acaaaatctt ctgaagattt gattaatttt
     2641 atagataaaa acactaaaaa gactgatagt atggtaattt tgcccgaagg tttggttata
     2701 aattttttaa tggagagaaa atcggatgat tattataatt ctttaatacc gctttattgg
     2761 gaagtttttg gcgatgaaaa actgatagaa tattttgaaa aaacaaagcc tgactatata
     2821 gttttcagca actgggataa taaggattat tacttcagat acatttgcag tgattatgcc
     2881 gtttcctttt gtaattatgt agcaaaaaat tacagacagg aaaaaattat tgataacggt
     2941 tttcgatatt taatttacag gaaaaaataa aaagccgtgc cactacctat cgaccggtgt
     3001 gaataaagtt ttaggggaat attatctcaa ttttaagctt acaatacccg caagtgatgc
     3061 cttagtgctg ctatgtttcc atcctgacac ggttcgacag cttacgccat tataatttaa
     3121 aacgtcaaca ataatatatc cgtccaccaa actcgcacta gcctcacagt aggctctgca
     3181 cctcgcataa agcgttcgag tcgagagatc cgcaacctcc ccgtggagca cggcttattt
     3241 ttattttaac atgaagaaag ttagtgaata ggtgaatagg tgcatttata ataaatttaa
     3301 ctgttcaact tatcgactct tcgacttttc aactaacttt ggagttcaaa caagtccttt
     3361 tcgacgccac aaatcggaca tgtccaatct gctgccaaat cttcaaattt ggttccttct
     3421 ttttcttcgt catataccca ttggcatact gtgcatacgt atcttgccat aattttctcc
     3481 tttttgttaa atttacccct agtaatcgat ttttctcatt gagaaataag atttaggatg
     3541 agcgcaaaca ggacaaactt caactgcttt tgtgctttct aaaatatgtc cgcagtttaa
     3601 acaaatccaa aatacttttt ctttcttttc gaaaacttct tcgtttttaa tgttgtcatg
     3661 aagtttttgg tatctttcgt tatggtcttt ttcgatttta gcaacacttt caaacagata
     3721 agctattttt tcaaaccctt cttctcttgc ttcttttgca aaagtcggat acatttgggt
     3781 ccattcgtaa ttttcaccgt ttgcagcatc ttgaaggttt gtcaaagtat ttgcaacctc
     3841 tccgccgtgc aataatttat accaaatttt tgcgtgttct ttttcgttat ttgcggtttc
     3901 ttcaaaaatg tgtgaaatct gtacatagcc ttcttttttt gcttttgccg catagtaagt
     3961 atatttgttt ctcgccattg attcgccggc aaaagcggct tctaaattct tttctgtttt
     4021 gcttcctttt aattccattt taatgccccc tttcaaaata tgtctttttt atattagcat
     4081 aaaaattctt aaggtaaata tttatgttaa aatagcctta tgaaaaatat tttagagcag
     4141 aaagtttatt attctgatac cgatgcttat ggagttgtgt ggcacgggtc gtatttaaga
     4201 tggcttgaaa tgggcagagt cgagctttgc gaggatatgg gctacaatct tatcgagctt
     4261 gaaaatcagg acattgtaat gcctgttgtt aatatcaatg tacgatacaa agcctctgcc
     4321 aagattaata atgagctgat tatcacgaca aaagtcaaag aatttaattc gatttgcgtt
     4381 aaatttgagc agaaaattac agataaaaac tcgggaaaaa tttttataga ggctcttgta
     4441 gaagttgtta cggttaacag agaaggcaaa ttgtaccgta aaatgcctca aatactgaca
     4501 gaagcttttg aaaaagcaat gaaagatgaa gaatatgcgc ttaaataaat atattgcagc
     4561 atcaggaatt tgttcaagaa gaaaagctga tgaattaatc caaagcggtg tggtttctgt
     4621 taacggaaaa aaagttgtag aattgggttt ttctgttaat gaaaaagata aagtttttgt
     4681 aaaaaatgaa cttattcgtc ctaaaaaatt tgaatattac agattttaca aacccacagg
     4741 atatattaca acaagcgacg atgaaaaaga cagaaaaatc atttatgacc tgcttcctcc
     4801 tgccatgcat aacctaaagc cggtcggaag gcttgacagg gattcaagcg ggttgataat
     4861 tcttaccaat gacggcgact tgattaatga attgacgcat ccttcaatta aagttccaaa
     4921 agtctatgtg gtaagtgtgg agggaaaagt ttcacttcat caccttgaaa aaatggcaca
     4981 gggaattgaa attgaaaaag gtaaaaacgc ttatgccgat actttaattc ttgaaagtac
     5041 aaataaaaaa acgattcttc aggtaactct gtatcagggt ttaaaccgtc aaataagaaa
     5101 aatgtttgaa aatttgggat ttgaagtaac ttcgctgaaa agaattcagc atgctacaat
     5161 aacattggaa ggtttaaaaa agggtcagtt taagccgata aaaccgcttc aggttaaaga
     5221 gttgagaaat tttttgtcta aattggagca aaaaaaatga ttaaagtcgc aattacagga
     5281 aatattgcct caggcaaaac cgcagtggaa gaaattttaa gaaaaaaagg ctaccaagtc
     5341 cttgatacag atgatatttc tcatacgttg cttgaagata aaaaggtcaa agagcaaata
     5401 ataagcgaat ttgaaaaatt tgatattgtc gaaaataatg aaatttcaag acataaattg
     5461 ggcaaaatag tttttacaga tattaaatta agaaaaaaac ttgaagaaat tttacatcct
     5521 ttaataaaaa aagaaataag gcgatttttt cgccaatgcg aatcaaaggg cgaaaaaatc
     5581 gcctttgttt ccgtcccttt gctttttgaa gtaaatatgc aaaaactttt tgataaaatt
     5641 gttttggtgc atgcagatac ccaatttagg ctgcagaggc ttgtaagccg aaacaatctg
     5701 actattgagc aggcaaaaaa cagaattaag atacaaatgc cgcaggatga aaaattgaaa
     5761 ttatcagatt atgttatata caacaataag acttctaaag aattagaatt agc
//
LOCUS       DAOQAU010000075         5551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_18367_length_5551_cov_7.538392, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000075 DAOQAU010000000
VERSION     DAOQAU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18367_length_5551_cov_7.538392"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKI94_08300"
     CDS             <1..180
                     /locus_tag="PKI94_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26771.1"
                     /translation="DGVTPKKETKYSQGKIPTILEKIFYRQNGTIEEIEKYGMDGGFI
                     ERKYYPLKKTFITYI"
     gene            306..809
                     /gene="rplI"
                     /locus_tag="PKI94_08305"
     CDS             306..809
                     /gene="rplI"
                     /locus_tag="PKI94_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNW26772.1"
                     /translation="MQVILRKDVQNLGEAGDLINVKDGYARNFLLPQKMAEVATDGAV
                     KNREQNLARIKAKQEKLHQQALEQAQKIEKVALLELSAKAGESGKLFGTITTKKLAEE
                     LLDKSGIDVDRKNLSLNAPINKIGEYKMLIKLTSKVKTEIKINVVASEIIKEELVEEI
                     KEETLEE"
     gene            848..1948
                     /locus_tag="PKI94_08310"
     CDS             848..1948
                     /locus_tag="PKI94_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26773.1"
                     /translation="MTTKKDFQLTAHSSRLTLNCLAIGSLPHKSVEDAIKLVSQNFQI
                     PFWPQLSKLNKNEDMIFQFLENMPGICTNDEKTYLENDSDEFFEQLECFFIDWEEVTL
                     NKNLEKLEKYGISEKNSATVVPFLSLIKKTLPDFAKGQIVGPFTLATSLTDKEGKCAF
                     YDETLREVIIKTLCLKALWQINEIKKASPETTPIIFIDEPSMSQLGTSAFITISKDEV
                     VDMIKEVSDLIKQNGAISAIHCCGKCDWSIPIQADVDIINLDGYFFAQNLSTFSKEVK
                     TFLQKGGKIAWGIIPTLDKDALEKADLTKMISKFEQAIGFLEQKGLNKDLLINNTLIT
                     PSCGAGSLTVELAQKAMNLTNELSKELKEKYK"
     gene            2107..2856
                     /locus_tag="PKI94_08315"
     CDS             2107..2856
                     /locus_tag="PKI94_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW26774.1"
                     /translation="MTIKLAITGKSGSGKTTVTKAFLRIFKEMFPEKSILLFDNDLSS
                     ELGHSFGLDIRNTIYGIRSGKHEYKTGIPQDMTKQEYIDWALEDIVVQLDENVDIIVS
                     WLVASKDCRCPITKQINDALVKFIERYDIVIFDCEFDLKYLNQLVDVEVDTALIVANP
                     TDESAHLAKRIEEFSAKYSAGGQIGVILNKVENTEITSVFELLKKYDLEILGVIPTDK
                     ELIHNSIEKDSELVQESIKQFYFRLNLPQGG"
     gene            2930..3769
                     /locus_tag="PKI94_08320"
     CDS             2930..3769
                     /locus_tag="PKI94_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNW26775.1"
                     /translation="MAIILDGKKLSEKILHELEEKISNLDKKPSFAVILVGNDPASEL
                     YVNIKEKKSKLLGINFSKITYPENIDEKTLLKKIEELNKDDKIDAMLVQMPLPKHINA
                     QNIIQAISPKKDADGITPENIGRISIGAEPFAYPCTPLGIVKLLEEYKIELQGKHAVI
                     IGRSNIVGKPLAQMLLNKNATVTICHSKTKNLSQITKTADILISAIGSPKFVKKDMVK
                     QNATVIDVGTSKSKGKTLGDVDFENVLPLSSYITPVPGGVGPMTIACLMKNVLEISRL
                     RLY"
     gene            complement(3747..4055)
                     /locus_tag="PKI94_08325"
     CDS             complement(3747..4055)
                     /locus_tag="PKI94_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26776.1"
                     /translation="MLPILLDLMNVISERNNCVFEQMVTANQRDYVQQQMADAIQQGA
                     DPDCDAMYELKKIDKNFEIKIAAYETQLKTLNAKYDSELKLVQEEAKKAGTINTGGGG
                     "
     gene            4368..>5551
                     /locus_tag="PKI94_08330"
     CDS             4368..>5551
                     /locus_tag="PKI94_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26777.1"
                     /translation="MKIEEKSLKSLEFDKIAEKLSSFARLEQSRDKCLGLSIYSDAKD
                     IKREIALTKEAKSILDVPLELPLEYVADINEIKNSTISSYLKEEEIVNTAKTIRTSRL
                     VKNFLKENLPQEALLYSMANLLFCDKELEDKIFSIIDNNLKIAQDATPELKSLYNSLK
                     DSQNNLKTRTNELLNNPDFSKHLQEQIYTMRDDRVVFQVRAASKSKVEGIIHDVSASS
                     QSFYIEPSQLVPLNNKIREIQAKIQAEIINILTILTKEIRVHIEEIQISEDLLAQIDF
                     HFAKALYAIKLKAVEPEIINEKVLKFESMRHPLLSDYVESVVENDFEIGKGYRSVIIT
                     GSNTGGKTVVLKTVGLFILMAKAGLFLPCLEAKIYPFEKVFADIGDEQSIAQSLSTFS
                     SH"
BASE COUNT         2064 a          996 c          992 g         1499 t
ORIGIN      
        1 gacggagtta ccccgaaaaa agaaacaaaa tattcccaag gaaaaatacc caccatactt
       61 gaaaaaatat tttacagaca aaacggaacc atagaagaaa tagaaaaata cggtatggac
      121 ggcggtttta tagaaagaaa atattatccg ctaaaaaaga catttataac ttacatataa
      181 tttttagggg tttgttctag gcaaacccta tttatttaat taatattaat ttactttccc
      241 ctcgacattt ttttttgttt ttagtacgat aaatacactg acaaacaaga aaaaaggaga
      301 atacaatgca ggtaatactt agaaaagacg tacaaaacct cggcgaagca ggggatttga
      361 taaatgtaaa agacggatac gcaagaaact tcttgcttcc gcaaaaaatg gctgaagttg
      421 caacagacgg tgctgttaaa aaccgtgaac aaaatttagc aagaattaaa gcaaagcaag
      481 aaaaattaca ccaacaagca ttagagcagg ctcaaaaaat cgaaaaagta gctcttcttg
      541 aattaagcgc aaaagcaggt gaaagcggaa aacttttcgg tacaattaca accaaaaaac
      601 ttgccgaaga acttcttgac aaatcaggca ttgatgttga cagaaaaaat ctttcactca
      661 acgcacctat caacaaaatc ggcgaatata aaatgcttat caaacttact tcaaaagtca
      721 aaaccgaaat taaaataaac gtagtcgctt ctgaaataat taaagaagaa ttagttgaag
      781 aaattaaaga agaaacttta gaagagtgaa cagtaatcag tgaacagtga acagaggagg
      841 aagaaaattg acaacaaaaa aggattttca gctcactgct cacagctcac gcctcacttt
      901 gaactgcctt gcaataggat ctctgccgca caagagtgtt gaagatgcca taaagcttgt
      961 cagccaaaat tttcagattc ctttttggcc gcaactttcc aagctgaata aaaatgaaga
     1021 tatgattttc caatttttgg aaaacatgcc gggcatctgc acaaatgatg aaaaaactta
     1081 tcttgaaaat gactctgatg aattttttga gcagcttgaa tgttttttta tcgactggga
     1141 agaagtaacg ctaaataaaa atcttgaaaa acttgaaaaa tacgggattt cagagaaaaa
     1201 ttccgcaaca gtagtaccgt ttttaagctt aataaaaaaa actttgcccg actttgcaaa
     1261 aggacagata gtgggtcctt tcacgcttgc gacttctctt actgacaaag aggggaaatg
     1321 tgctttttat gatgagactc taagagaagt tatcattaaa accctctgct taaaagccct
     1381 ttggcaaata aacgaaataa aaaaagcatc ccccgaaaca accccgataa tttttattga
     1441 cgagccttcg atgtcacagc ttggcacctc ggcttttata acgatttcta aagacgaggt
     1501 tgttgacatg ataaaagaag tttccgattt gataaaacaa aacggagcaa taagtgccat
     1561 tcactgttgc ggaaagtgcg attggagtat ccctattcaa gcggatgttg atataataaa
     1621 tcttgacggc tacttttttg cccaaaattt aagcactttt tcaaaagaag ttaaaacatt
     1681 tttgcaaaaa gggggcaaaa tagcatgggg gattattcct accctcgata aagatgccct
     1741 tgaaaaagca gacttaacaa aaatgatttc aaaatttgaa caagcaatcg gatttttgga
     1801 gcaaaaagga ctcaacaaag atttattaat aaataacact ctgataacac cctcttgcgg
     1861 tgcaggctct ttgacggtag aattagcaca gaaagcaatg aatttaacaa atgaactttc
     1921 aaaagaatta aaggaaaagt ataaataatt gtcatgctga atttatttca gcatctttcc
     1981 ggctttgtat atcggataga tcctgataca tcgcaggcga tactctccct tgtaactctc
     2041 gctaaagctc gagaacaaga agagaagaca agttcaggat gacacaacat taaaggagaa
     2101 atatatttga caattaagct ggcaatcaca ggaaaaagcg gaagcggaaa aacaacggtt
     2161 acaaaagcat ttttgagaat ttttaaagaa atgtttcccg aaaaatcaat tttgcttttt
     2221 gacaacgacc tttcaagcga acttggacac agtttcgggc ttgacataag aaacacaatc
     2281 tatgggataa gaagcggaaa acacgaatac aaaaccggaa tcccgcaaga tatgacaaaa
     2341 caggaatata ttgactgggc actcgaagat atagtcgtac aactggacga aaatgtcgat
     2401 ataatagttt catggcttgt cgcttcaaaa gactgcagat gcccgattac aaaacagata
     2461 aacgacgctc tggtaaaatt tatcgaaaga tatgatatag taatttttga ctgcgaattt
     2521 gacttaaaat acttaaatca gcttgtggat gttgaagttg acaccgcatt aatcgtggca
     2581 aacccgacag atgaatcggc acatcttgca aaaaggatag aagaattcag cgcaaaatac
     2641 tcggcaggcg gacaaatcgg tgttatactc aataaagtag aaaacacgga aatcacatcg
     2701 gtatttgaac ttttgaaaaa atatgacctt gaaattttag gcgtaatccc tactgataaa
     2761 gaactcatac acaattcaat agaaaaagat tccgaattgg ttcaagaatc aataaaacag
     2821 ttttatttca gattgaattt accgcaaggg ggataatcag tgagctgtga gcagtgaacg
     2881 gcaaacggga tagcaaaaat cccgccaaca aaaacaaagg gggcaaaaaa tggcaattat
     2941 tcttgacgga aaaaaattaa gcgaaaaaat tcttcacgaa ttggaagaaa aaatttccaa
     3001 tcttgacaaa aagccttctt ttgcggtgat tttagtaggg aatgaccctg caagcgagct
     3061 ttatgtaaac ataaaagaaa aaaaatcaaa acttttggga ataaattttt caaaaataac
     3121 ttaccctgaa aacattgacg aaaaaacttt gttgaaaaaa attgaagaat taaacaaaga
     3181 tgacaaaatt gatgctatgt tggtccaaat gccgcttcca aaacacatta atgcgcaaaa
     3241 tataattcag gcaatttcac ccaaaaaaga cgccgacggg attacaccgg agaatatcgg
     3301 aagaatttcg ataggcgcag aaccttttgc ctacccttgc actcctttgg gaattgttaa
     3361 acttttggaa gaatataaaa tagaacttca aggcaaacac gccgttatta taggacgctc
     3421 aaatattgtc ggaaaacccc ttgctcaaat gcttttaaac aaaaatgcaa cggtcacaat
     3481 ctgccacagc aaaacaaaaa acctttcaca aataacaaaa accgccgata ttctaatttc
     3541 agcaataggc agtccaaaat ttgtgaaaaa agacatggta aaacaaaatg cgacagtaat
     3601 agatgtagga acttccaaat caaaaggcaa aacgctcggt gatgttgatt ttgaaaacgt
     3661 tttgccttta agttcttata taactcccgt gccgggaggt gtgggtccga tgaccattgc
     3721 ctgcctgatg aaaaatgttt tagaaattag ccgcctccgc ctgtattaat tgttccggct
     3781 tttttagctt cttcttgtac aagctttagt tcagaatcat atttggcatt taaagttttc
     3841 aactgtgttt cataagcagc aatctttatt tcaaaattct tatcaatttt cttaagttca
     3901 tacatcgcgt cacaatccgg atccgcacct tgctgtatag catcagccat ttgttgttgt
     3961 acataatctc tttggttagc tgttaccatt tgctcaaata cacaattatt tctttcgcta
     4021 ataacattca ttaaatctaa taaaatcgga agcatctctt ttctccttgt tgtatatctt
     4081 atatatataa agtatatact attcaaaata ttgcccactt tgtcttgttt gttaacaatt
     4141 tgtaacatta tgtttaaaaa ccaatatatt gcttgcattt gaggttatgt ttattctgcg
     4201 gctgcggtaa aattaaaatt tttctcgcag ccgccctagt ccagcgggag ccaagctacc
     4261 cttttaccaa ggctatgctt ggctcccgcc ttcttaaggt cgggcacgaa aaacactaaa
     4321 atttgtaaaa ttttgtattt ttccgtgccc tcaactataa taagtttatg aaaatagaag
     4381 aaaaaagttt aaaatcactt gaatttgata aaattgccga aaaactttca agctttgcaa
     4441 ggctcgaaca aagcagggac aaatgtttag ggctaagtat atactccgat gcaaaagata
     4501 taaaaagaga aatcgctctg acaaaagaag ccaaaagcat tttggatgtt ccgcttgagc
     4561 ttcctttaga atatgttgcc gatataaacg agattaaaaa ttcaacaata agtagttatt
     4621 taaaagaaga agaaatcgta aataccgcaa aaacgataag aacttcaagg cttgttaaga
     4681 attttttgaa agaaaatttg ccgcaagagg cacttttata ctctatggca aatctacttt
     4741 tttgcgataa agaactcgag gataaaattt tttctataat tgacaataat ttaaaaatag
     4801 cacaagacgc aacccctgaa ttaaaaagct tatacaattc gctcaaagac agccaaaaca
     4861 atctaaaaac aaggacaaac gaacttttaa acaaccctga tttttcaaaa catttgcagg
     4921 agcagattta taccatgcga gatgacaggg tagtctttca ggtaagagca gcttcaaaaa
     4981 gcaaggtgga agggattatc cacgatgttt ccgcaagcag ccagtcgttt tacattgagc
     5041 cttcgcagct tgtgcctttg aacaataaaa taagagaaat ccaagcaaaa attcaggcag
     5101 aaatcataaa tatccttaca attttaacaa aagaaataag agtgcatatt gaagaaatac
     5161 aaatttcaga agacctgctg gcacaaatcg attttcactt tgcaaaagcc ctctatgcaa
     5221 taaaacttaa agcagttgag cccgagatta ttaacgaaaa agttttaaaa ttcgaatcaa
     5281 tgcgccaccc tttgctttct gattacgttg agagcgttgt cgaaaacgat tttgaaatag
     5341 gaaaaggcta caggtcagtt ataataacag gttcaaacac cggcggaaaa accgtcgtgc
     5401 taaaaaccgt agggcttttt atcctaatgg caaaagcagg attgtttttg ccgtgcttag
     5461 aggcaaaaat ttatcctttt gaaaaagttt ttgccgacat cggcgatgag caaagcatag
     5521 cccaaagcct ttcgactttt tcttctcata t
//
LOCUS       DAOQAU010000076         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_19554_length_5313_cov_5.854127, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000076 DAOQAU010000000
VERSION     DAOQAU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19554_length_5313_cov_5.854127"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..794)
                     /locus_tag="PKI94_08335"
     CDS             complement(96..794)
                     /locus_tag="PKI94_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26778.1"
                     /translation="MFDRFGSIALNTTGLNSNPYNLFSENINSEKDIFCPVGSDSLFT
                     EGIVPIDTNFKLFFDNDFMSSLMLFNLFTKKMMTLKQQSEIITTNFNTNTNLPALKSV
                     YNAELGNTFANIANKNARSTGTIGKCLKGVRLALDWAGLHKGGSMGASAYQAVGNLSN
                     NKNFKQVQVSRNDLKNLPAGCIIVWNRTSKNPHGHIAVTLGNGKEASDHVQNLITNMN
                     ASFTVFAPTKGQIA"
     gene            complement(1152..1775)
                     /locus_tag="PKI94_08340"
     CDS             complement(1152..1775)
                     /locus_tag="PKI94_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26779.1"
                     /translation="MKKYLFTAALCLAFSATSALAACPLNSEKCPCPNNPDLKNPPQK
                     EFKMPDKDAKKRFEAKMIKERQLMYNALNMTEEQKTKAEVLDKKNREEAKKIMENIRC
                     EKNKLRDLKEKKTCPCLVMQQKHELRKAKKELKKHFCKSRQEFEAILTKEQQAKLKIL
                     REEKRAEMMKKGYPDHKKHHGHRFMGSDAPPPPPFGCPMDSEKPMKK"
     gene            1886..2245
                     /locus_tag="PKI94_08345"
     CDS             1886..2245
                     /locus_tag="PKI94_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNW26780.1"
                     /translation="MEEINLRNYAHLGDAVWELFVREITILKTQKAKELHKLTTDKVN
                     AKFQHELLLKIQNFLSEEEIELTRRGRNLPIPVGRKSNQSEYRMATAFEVLIGRLYIK
                     DKTRLDEILTKIKDFLE"
     gene            2343..2415
                     /locus_tag="PKI94_08350"
     tRNA            2343..2415
                     /locus_tag="PKI94_08350"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2376..2378,aa:Ala,seq:tgc)
     gene            complement(2489..2782)
                     /locus_tag="PKI94_08355"
     CDS             complement(2489..2782)
                     /locus_tag="PKI94_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26781.1"
                     /translation="MKKIVKTLVIIFFVSLFICPAKAAAPDEKKEVVILQSELPSQQS
                     QSKYKLSKPKSTSSEEANNFAEEAEGAQDYTQIMNTVNSLVAPFVNFSNYEKR"
     gene            complement(2859..3929)
                     /locus_tag="PKI94_08360"
     CDS             complement(2859..3929)
                     /locus_tag="PKI94_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26782.1"
                     /translation="MNNKSIEKISYKGILVMFALGFSFLGNLAFASDNVLQAIQINGN
                     KDTYDIVLKSDDSAELHKTIQAPNKMVLHLKGIRASKSINTIYSNTASVDSVVVEPIN
                     NETVKILIQADNVGNANVSFDTLKTPLGLLGGSLQTTTGKQIELSKPMNTYKSVYEEG
                     LDENSESFLSGLSSSTLGRYIKKALKSSHLSWMIAFGLFTIIVMGGIKNLKGQDNTIK
                     VGLSQSLRERELESYRGLSSTEENLNHLPLPSIAPQMAGANYGLKAYQSGTRSPYMTH
                     DIQRPAARPTITPTKVPVGAGLKTSQTTAQSRTISNTRPKASNIDSVKFLESMTKIYE
                     KNGRSDLAQGLKTNMKKAGVMG"
     assembly_gap    4163..4172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4260..5000
                     /locus_tag="PKI94_08365"
     CDS             4260..5000
                     /locus_tag="PKI94_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26783.1"
                     /translation="MEQEKLKISIAHLYPKLLNIYGDFGNVLTLKNRCEWRGIEVELT
                     QVNIGDKIDPEKFDIYFIGGGQDKQQVEVSRELQFQKDALHFAMNEGAVFLGICGGYQ
                     LFGHYYQPHNGARLLGLGLLDAYTVAGNKRFIGNVTAKTDYVIPKTLVGFENHSGLTY
                     IQGETKPLAIVEIGAGNNNKDKTEGARFKNVFGTYLHGSFLPKNPHFADYLIKLALEK
                     RYKNPVDLEKLDDELELKAHISLINKPY"
     gene            complement(4997..>5313)
                     /locus_tag="PKI94_08370"
     CDS             complement(4997..>5313)
                     /locus_tag="PKI94_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26784.1"
                     /translation="YLATLSDRKSSGLNGNLTQVCGTVMIDVNGPQKGPNSIGRDVFV
                     FHIANGKGPKLHPYGSSETSSWWKDPDTNQIRYCDPADINTNNGSACTARIIEEGWQM
                     NY"
BASE COUNT         1557 a          877 c          966 g         1903 t
ORIGIN      
        1 aattatcaga ttatgttata tacaacaata agacttctaa agaattagaa ttagcaatta
       61 actctcttga aaaaacatta aacttgcttt aatgtttatg ctatttgacc ttttgtaggc
      121 gcaaagacag taaaagaagc attcatgttt gtaataaggt tttgaacatg atcgcttgct
      181 tcttttccgt tgcctagagt taccgcgatg tgtccgtgag ggttttttga tgttctgttc
      241 caaacaatta tgcagcctgc aggtaagttt ttcagatcat ttcttgacac ttgaacttgt
      301 ttgaaatttt tattatttga aagatttcct actgcttgat acgcagatgc tcccattgaa
      361 ccgcctttat gtaagcctgc ccaatcaagt gcgagcctta ctccttttaa acattttcct
      421 attgttcctg tcgagcgggc atttttattt gctatgtttg caaaagtatt tccaagctca
      481 gcgttataaa cactttttaa agcaggaaga tttgtattgg tgttgaaatt tgttgttatt
      541 atttctgatt gctgttttaa tgtcatcatt ttttttgtaa aaaggttaaa aagcataaga
      601 gaagacataa aatcattatc aaaaaataat ttaaaattcg tatcaatcgg cacaatgcct
      661 tcagtgaaaa gagaatcaga gccaacaggg caaaaaatat ctttctccga atttatattt
      721 tcggaaaata aattgtaagg gttagaattt aaccccgtag tatttaatgc aatagaacca
      781 aacctgtcaa acataaacct ttaatcccaa ataaacctta tatattatat aaagtatttt
      841 tcttgaaaaa aattgcctaa atttcaaaaa ttattttttt atgatgaaca aaaagtatcc
      901 gactataagg tttagtccga taataagtat tataaatatt gattttatta tgttttttag
      961 ggttgtctga atttgcattg tttgatgata actcttaaga attattacat aaccgacaaa
     1021 agtatataag aaaaaattta atctgctcag ttgggtaata attttttaaa aaattatagc
     1081 cgtaaaggat aacaacaggg cgggattttc ctttaggaaa atcccgccct gttgttattt
     1141 ttaaataaga attatttttt catgggtttt tcagaatcca ttgggcagcc aaaaggaggt
     1201 ggaggtggtg catcagagcc cataaacctg tgtccgtggt gttttttgtg atcagggtat
     1261 ccttttttca tcatttctgc tcttttttct tctcttagga ttttaagttt agcctgttgt
     1321 tcttttgtca aaattgcttc aaattcttgt cttgatttgc aaaagtgttt tttaagttct
     1381 tttttagctt ttcttagttc gtgtttttgt tgcataacca aacaaggaca agtttttttc
     1441 tcttttaagt ctcttaattt atttttttcg catctgatat tttccattat ttttttagct
     1501 tcttctcgat tttttttatc aagaacttca gcttttgtct tttgctcttc ggtcatatta
     1561 agagcgttgt acataagctg tctttcttta atcattttag cttcaaatct ttttttagca
     1621 tctttatctg gcattttaaa ttctttttgc ggtggatttt taaggtcagg attatttgga
     1681 caaggacatt tttcagaatt tagcgggcag gcggcaagtg ctgatgttgc actaaaagct
     1741 aagcacaaag ctgcagtaaa taagtatttt ttcatataat tctccttccg gtattcaaaa
     1801 taccaattat cggacagtta gattttaacg ctaaaaaata taaaatcaat aagcaaaaag
     1861 atttatttca tgctaggatt taggcatgga agaaattaat ttaagaaatt atgcgcattt
     1921 gggcgatgcg gtgtgggaat tgtttgtaag agaaatcacc atacttaaaa ctcaaaaagc
     1981 aaaggaattg cacaagctta cgactgataa agttaatgca aaatttcagc atgagctttt
     2041 attaaagata cagaattttt taagtgaaga agaaattgaa ttaacaagaa gaggaagaaa
     2101 tttgcctatt ccggtcggga gaaaatcaaa tcagagcgaa tacaggatgg caactgcatt
     2161 tgaggtttta attgggcggt tatatattaa agataagact cgcttagatg agattttgac
     2221 caaaatcaag gattttttgg agtaaatata tttacaaaaa aaatttaggg agtataattt
     2281 tagttgaacg gcagtttatt ttagtcgaaa taattgaaaa tttaatagta aaaaattgct
     2341 ccgggggatt agctcagttg gtagagcgcc tgccttgcac gcaggatgtc gcaagttcga
     2401 atcttgtatc ctccaaaaac aaagaccgca tctacgatgc ggtctttgtt tttggcagta
     2461 ttttttaaga cgagaattac caagtcaatt atcttttttc gtagttgctg aaatttacaa
     2521 aaggtgcaac caaagagttt actgtgttca ttatttgagt ataatcctgc gcgccttcgg
     2581 cttcttcggc aaagttgttc gcttcttcgc ttgatgtgga tttgggcttg ctcaatttgt
     2641 atttggattg actctgttgc gaaggcagtt cggattgcaa aattaccact tctttttttt
     2701 catccggagc cgctgctttt gcaggacata tgaaaaggct tacaaaaaat ataattacaa
     2761 gtgtttttac aatctttttc atgccaatat tatagcgcaa aaaaaaggga gtttttagcc
     2821 ccctcgtaat atgttgagta ttgttatctg tttctttttt agcccataac tccggctttt
     2881 ttcatatttg ttttaagccc ttgtgccaaa tcagagcgtc cgtttttttc ataaattttt
     2941 gtcattgatt ccaaaaattt cacactgtct atattggaag ctttgggcct tgtattgctt
     3001 atagttcttg attgtgcagt cgtttgtgaa gtttttaatc ctgcacccac agggactttt
     3061 gtcggtgtga ttgttggtct tgctgcgggc ctttgaatgt cgtgtgtcat gtaaggactt
     3121 cttgttccgc tttgataagc ttttagtccg taatttgcac ctgccatttg tggagcaata
     3181 cttgggagtg gtaaatgatt taaattttct tctgttgaag aaagtcctct gtagctttca
     3241 agctctcttt ctcttaaact ttgtgacagg cctactttaa ttgtattatc ttgtcctttg
     3301 aggtttttaa ttccgcccat tactattatt gtaaacagtc caaatgctat catccagctt
     3361 aagtggctgc tttttaatgc ttttttaatg tatcttccca aggtgcttga tgaaagtccg
     3421 cttaaaaaac tttcggagtt ttcatctaat ccttcttcat aaacggattt gtaagtattc
     3481 atgggtttgc ttaattctat ttgttttcct gttgttgttt gcagtgagcc gcccaaaagg
     3541 cccaacggag tttttagggt atcaaaactt acgtttgcat tgcctacatt atctgcctga
     3601 ataagaattt ttaccgtttc attgttgata ggttcaacta caacactgtc aacagaggcg
     3661 gtgttactat aaatcgtgtt gattgacttt gaggctctta tacctttaag atgaagcacc
     3721 attttatttg gggcttggat agttttgtga agctctgcgc tgtcatcaga ttttaaaact
     3781 atgtcgtatg tatctttgtt tccgttaatc tgtatcgctt gaagcacatt gtcagatgca
     3841 aatgcaaggt ttcctaaaaa cgaaaaacct aatgcaaaca ttaccaaaat gcctttgtag
     3901 cttatttttt caatactctt gttgttcact tccctactcc cttgtaactt tctcctcaac
     3961 gtattttgaa atttactcaa cttcattata aagtgtactt tttttggctt catgtatcat
     4021 caatcagggg atttaacttt ttattgacca cttggtctat gaaaagtgag gagtgagagg
     4081 tgagaagtgt gtggattaaa agttgaaaag tcgaatagtc gaatagttga aaagttaaaa
     4141 agttaaaaag ttaaatttat tannnnnnnn nnaattgcac ttattcactt atttacctat
     4201 tcacttctat ttaccattta ctatttacta tttactcccg tttataatat aattttttta
     4261 tggaacagga aaagttaaaa atctccatag cgcatcttta tccgaagctt ttgaacattt
     4321 acggggattt cggcaatgtt ttaacactca aaaaccgttg tgaatggcgc ggaattgaag
     4381 tcgaattgac gcaggtaaat atcggcgaca aaattgatcc tgaaaaattt gacatttatt
     4441 ttatcggggg cggacaggac aagcagcaag ttgaggtttc aagggagctt cagtttcaaa
     4501 aagatgcact tcattttgcg atgaacgaag gggccgtctt tttgggaatt tgcggcggct
     4561 atcagctttt tgggcattac tatcagccgc acaatggtgc aagacttttg ggattggggc
     4621 ttttagatgc ctataccgtt gccggaaaca aaaggtttat cggaaacgtt acggcaaaaa
     4681 cggattatgt tataccaaaa acccttgtag gttttgaaaa tcacagcgga ttgacttata
     4741 ttcagggcga aacaaagccg cttgcaatag ttgaaatcgg tgcgggaaat aataacaagg
     4801 acaaaaccga aggagcgagg tttaaaaatg tttttggcac ttaccttcac ggttcttttt
     4861 tgcctaaaaa ccctcatttt gctgattatt tgattaaact tgctcttgaa aaacgctaca
     4921 aaaatcctgt tgaccttgaa aaattagacg atgaacttga gctaaaagcc cacatttcgc
     4981 taatcaataa accgtactaa taattcattt gccaaccttc ttcaatgata cgcgcagtgc
     5041 aggcagaacc attattcgta tttatgtctg caggatcaca atatctaatt tggtttgtat
     5101 ccggatcttt ccaccaacta gatgtttcag agcttccata aggatgtaac ttaggacctt
     5161 taccatttgc tatatgaaaa acaaaaacat ctctaccaat gctgttaggt cctttttgag
     5221 gaccgttaac atctatcata actgtaccac aaacttgagt taaattacca tttaaaccag
     5281 aagattttct atctgataga gttgccaagt atg
//
LOCUS       DAOQAU010000077         5309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_19577_length_5309_cov_6.230110, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000077 DAOQAU010000000
VERSION     DAOQAU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19577_length_5309_cov_6.230110"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1085)
                     /locus_tag="PKI94_08375"
     CDS             complement(75..1085)
                     /locus_tag="PKI94_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNW26785.1"
                     /translation="MVSKAMVMAAGVGSRLDPLTKNVPKPLVPIANRPVMDILIERLL
                     EVYVKDVIANTHYLSDKIVNHYKKSKINVNFNYIYEKELSGTAGGLKKCQFFFDKGED
                     FLVLSGDGLSNADFATGIKSHKKSGAIATIGIKKVPMEEIPNFGVVVTDKNGFVKTFQ
                     EKPSIKEAKSDKISTGIYIFNYEIFNYIPKGTFYDFAKNVFPDLMSKGLKINTFNVDE
                     YWSDIGTLDQYFQSTKDLFDGLYKINHSKIIKTDKGSYISDKSYIAPSAKLSGFNTIG
                     KDCVIGKNVKLENCIIWDNVEIKDNVALKDCIIASNCEINTDMINQIIGPNQIISEGI
                     YK"
     gene            1216..2400
                     /locus_tag="PKI94_08380"
     CDS             1216..2400
                     /locus_tag="PKI94_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26786.1"
                     /translation="MRKSSGVLKNRRAQQMVEFLLVMPFIIILFGILTEYVYALTINM
                     NLNQGLKSVTSLIYGQINPGVDKEQVRDNFVKYWLRNYLRSRYAPANSENELNVDYRI
                     VGEDAVFIASYKYIPSFTLPNIYFHFLPNSMRFSAISVIPSVLLKENIYDGSTTENIY
                     DGSTTNIVLWGAPVQEANKHGVMELTGNTTGAIVDSIIFLIPKAIAGVPLTDKTYEVK
                     KWNGENYRYLSTSNLAVNLTDKKPYSCNSDFSSCSEISGIPNFISYFPNTVTNIFFYD
                     DYSDALPWVEHPNASISSILNSGNLKNALALVENNTYKSVGNYDDLDILGYNTSLLPS
                     PPVNNHIFSDSGKYVVETYGSLVIVHNGNQTKAELNSILNNLPVPSYSATDFSSEFGV
                     LE"
     gene            2397..3596
                     /locus_tag="PKI94_08385"
     CDS             2397..3596
                     /locus_tag="PKI94_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNW26787.1"
                     /translation="MKKKNASTVMYMIFLFSVILAFCAMAIDATIIYNTRMKLQDATE
                     RAALAGAELFNDKFYPTGTLAATIESAAEEKAEEIFGHFLFGDLKSVNTTIIADAKYS
                     NKKILVKTSAYSPTFFLSFLGVQSVKINAKACAVSEKMDVKSNFDSAMWTTPKALYRS
                     GIIYSEDNSTDTSISKPLGQISSIQNFYSASYETNTPIFRLIDDIDNHPLSLGPGGYL
                     LIRLPAPIVDKPGPDLFIKEAGKALEGYFVFAGIDIDPFNPYLNNATQGGGVAWKNIT
                     CSAISEYEDLNNNLVPYSVSTTHSGTQEKFYGSAYFDLGDSCIKTSAGNNANLSMAKY
                     IKIVDDNSESAFVKRSLGSSLPSSPPYSYYKAMLFGEASTATAGADIDAIQILNFVRL
                     IPPPEHL"
     gene            3706..>5309
                     /locus_tag="PKI94_08390"
     CDS             3706..>5309
                     /locus_tag="PKI94_08390"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNW26788.1"
                     /translation="MKKIFGGKAMLTALFSLICATSPVFASEAALNVPNIQAANPAYY
                     NLLLIGIAVSVVGLLFGFIEFLKVKAIKAHKAMTDVGETIFATCKTYLLQQGKFLIAL
                     EILIGLCIAYYFGVLQKMSVNEVLTILGWSVIGILGSYSVAWFGIRMNTLANARTAFS
                     ALKGNPLNILNIPLKAGMSIGVCLVSVELIMMLIILLFVPGHLAGACFIGFAIGESLG
                     ASALRVAGGIFTKIADIGSDLMKIQFGIKEDDPRNPGVIADCTGDNAGDSVGPTADGF
                     ETYGVTGVALVSFILLGVFKDYQVALLTWIFTMRFLMIITSVVSYWANSIFDSLFSGK
                     IVKFDFEGSLTRLVWITSILSIVMTFSVSKWLLAPLGGNLWLILSGIISCGTLGAALI
                     PEFTKIFTSPKAKHTEEVVRASREGGASLTILSGLVSGNFSGFWIGMVVVMLMGGAYM
                     FSTYIPVEMMIYPSVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSLIEEIP
                     NVKEEIEKDFGFTPDFENAKQYLEEN"
BASE COUNT         1555 a          850 c         1025 g         1879 t
ORIGIN      
        1 ttacaggaaa gatacaaaaa tcgcccgatt ggggaggaaa agtgaaaagt aaaaattttt
       61 gttttcgttt ttatttattt ataaattcct tcggaaatta tttgattcgg ccctatgatt
      121 tggttaatca tgtcagtgtt tatctcgcag tttgaagcta ttatgcagtc ttttagcgca
      181 acattatctt ttatttcgac attatcccag attatacaat tttcaagttt tacatttttg
      241 ccgataacgc agtcttttcc tattgtatta aatccgctaa gctttgcgct gggtgcaata
      301 tagcttttat cgcttatgta agaacctttg tcggttttta ttatttttga gtgatttatt
      361 ttgtataaac cgtcaaacaa atcttttgtt gattgaaagt attggtcaag ggttcctatg
      421 tcactccagt attcatctac gttaaaagta ttaatcttaa gccctttaga catcaaatcg
      481 ggaaaaacat tttttgcaaa atcataaaaa gtgcctttgg gaatataatt aaaaatttca
      541 taattaaaaa tgtaaatacc ggtgcttatt ttatcgcttt ttgcttcttt tattgaaggc
      601 ttttcttgaa atgtttttac aaatccgttt ttatcagtta caacaactcc gaaattgggg
      661 atttcttcca taggtacttt ttttattcca atagtagcaa tcgcaccgga ttttttatgg
      721 gatttaattc ctgttgcaaa atcagcattt gaaagcccgt cgcctgagag tactaaaaaa
      781 tcttcaccct tgtcaaagaa aaattggcat tttttaagcc cgccggctgt tcctgacaat
      841 tctttttcat aaatgtaatt gaaatttaca tttattttgg attttttgta gtggttgact
      901 attttatcag aaaggtaatg agtgtttgca attacgtctt ttacgtaaac ttctaaaagc
      961 ctttcaatta aaatatccat aaccggtcga tttgcaattg gcacaagcgg ttttggaaca
     1021 ttttttgtga gcggatcaag tcttgaaccc acacctgctg ccattaccat tgcttttgaa
     1081 accatgaaaa cctcccttat tattttgatt ttataacaaa aacatttttt ttcaattttc
     1141 ttaagttaag acagataata ataataaaaa ttgtaaattt ttgtattttc agcgtcctca
     1201 gtgtaaaatg ttggcatgag aaaaagttca ggagttttaa aaaacaggcg ggcacagcaa
     1261 atggtggaat ttttgcttgt aatgcctttt attataattc tttttgggat tttaacagaa
     1321 tatgtctatg ctttaaccat taatatgaat ctgaatcagg gcttaaaatc tgtaacttct
     1381 ttaatttatg gtcaaattaa tccgggagtt gataaagagc aggtcagaga taattttgta
     1441 aaatattggt tgagaaatta tttgcgctca aggtatgctc cggctaattc tgaaaacgaa
     1501 cttaatgttg attatagaat agtaggcgaa gatgcagttt ttattgcatc ttataaatat
     1561 attccttcat ttactttgcc aaatatttac tttcatttcc ttcccaattc aatgcgcttt
     1621 agtgctattt ctgtaattcc gtcggttctt ttgaaggaaa atatttatga tggttctaca
     1681 acagaaaata tttatgatgg ttctacaaca aatatagttt tatggggcgc ccctgttcag
     1741 gaagcaaata agcacggcgt tatggagttg acaggaaata ctacaggggc tattgttgat
     1801 tcaataattt ttcttattcc gaaagctatt gcgggggttc cacttacaga taaaacttat
     1861 gaggttaaaa aatggaacgg tgagaattat cggtaccttt caacatcaaa tttggctgtg
     1921 aatttaacag ataaaaaacc atattcttgc aattccgatt tttcgagttg ttcggaaata
     1981 tcaggcattc ctaactttat ttcttatttt ccgaatacgg taacaaacat ttttttctac
     2041 gatgattatt cagatgcttt gccttgggta gagcatccta acgcatcaat tagctcaatt
     2101 cttaattccg gaaatttgaa aaatgcttta gctttggttg aaaataatac ttataaaagc
     2161 gtgggcaatt atgatgattt ggatatttta gggtataata catctttatt gccgtctccg
     2221 cctgttaata atcatatttt ttcagattcg ggcaagtatg ttgtggaaac ttacggttct
     2281 ttagtgattg ttcataatgg aaatcaaacg aaggcagagt taaattctat tttaaacaac
     2341 ttacctgtcc cttcatattc tgctacggat ttttcaagtg aatttggagt tttagagtga
     2401 aaaagaaaaa cgccagcact gttatgtata tgattttttt atttagcgta attctagctt
     2461 tttgcgcaat ggcaattgat gcaactatta tttataatac aagaatgaag cttcaggatg
     2521 ccacagaaag ggcggctttg gcaggagcag agttatttaa cgataaattt tatcctacag
     2581 gaactttagc agcaacaatt gagtcggcag ctgaagaaaa ggcagaagag attttcggac
     2641 attttttgtt tggggattta aaatctgtta atacaacaat aatagcagat gcaaaatatt
     2701 cgaataagaa aattttggtt aagacaagtg cttattctcc tacatttttc ctttctttct
     2761 taggtgttca gtcggtaaag attaacgcaa aggcttgtgc ggtaagcgaa aaaatggatg
     2821 taaaaagcaa ttttgattct gctatgtgga ctactccaaa agctttatat cgttccggta
     2881 ttatatacag cgaggataat tctacagaca catcaatatc taaacctttg ggacaaatct
     2941 catcaattca aaatttttat tcggcaagtt atgaaacaaa tactcctatt tttagattaa
     3001 ttgatgatat tgataatcat cctttaagcc ttggacccgg cggatatttg ctgataaggc
     3061 ttcctgcgcc gattgttgat aagccgggac cggatttatt tataaaagaa gcaggtaaag
     3121 cccttgaggg gtattttgtt tttgcaggta ttgacattga cccttttaat ccttatttaa
     3181 ataatgcaac acagggaggc ggtgttgcat ggaaaaatat aacttgttca gccatttccg
     3241 agtacgaaga tttaaataat aatcttgtgc cgtattctgt cagcacaact cattcaggga
     3301 cacaggaaaa attttacggc agtgcatatt ttgatttagg tgattcttgt attaaaacaa
     3361 gtgcaggtaa taatgcaaat ctttccatgg caaagtatat taaaattgtc gatgataaca
     3421 gcgaaagcgc ttttgttaaa aggtctttgg gctcttcttt gccttcatcg cctccctaca
     3481 gttattataa agcaatgttg ttcggtgaag cttctaccgc aacggcgggt gcagatatag
     3541 acgcaataca gattttaaat ttcgtaagat taattcctcc gcccgagcac ctttaatcta
     3601 aaactttttt ctgttaagtg attgtaaata tttgatttaa tagttaaaga caattataat
     3661 atttcttgta tataacgtat attttttatt tgaaggagag tagttatgaa aaaaattttt
     3721 ggaggcaaag caatgttaac agccctgttt tcgttgattt gcgccacttc gccggttttt
     3781 gcaagcgaag cggcattgaa tgttccgaat attcaagcgg caaatcctgc ttattacaat
     3841 ctcttgttga ttggtatcgc agtgtctgtt gtaggtttgt tgttcggatt tattgagttt
     3901 ttaaaggtta aggcgattaa agctcacaaa gctatgactg atgtgggtga aaccattttc
     3961 gcaacctgta aaacttatct tttgcaacag ggaaaattcc ttattgcgct tgaaatactt
     4021 ataggcttgt gtattgccta ttatttcggt gtgctgcaaa aaatgagtgt aaatgaagtt
     4081 ttaaccatat taggttggtc tgtaataggt attttgggct cttattctgt ggcatggttt
     4141 ggtatcagaa tgaataccct tgcaaatgca agaactgctt tcagcgcgtt aaaaggtaac
     4201 cctcttaata tcttgaacat accgttaaaa gcaggcatga gtataggtgt ttgccttgtt
     4261 tcggttgagc ttataatgat gttaattatc ttgttatttg tccccggaca ccttgcaggc
     4321 gcatgcttta tcggttttgc aatcggtgaa tcgctgggtg catctgcgct tcgtgtagcc
     4381 ggcggtatct ttacaaaaat tgccgatatc ggttcagact tgatgaaaat tcaattcggt
     4441 atcaaagaag atgatcctag aaaccccggt gtaatcgccg attgtacagg tgataacgca
     4501 ggtgattcag taggtcctac cgcagacggg tttgaaactt acggtgtaac aggtgttgct
     4561 cttgtaagct ttattctttt aggcgtgttt aaagattatc aggttgctct gttaacctgg
     4621 atttttacaa tgagattctt gatgattatc acatcagttg tttcttactg ggcaaacagt
     4681 atttttgaca gtcttttctc aggaaaaata gttaaatttg attttgaagg ttctttgacc
     4741 cgcttagttt ggataacttc aattttgtcc attgtaatga ctttctcagt aagtaaatgg
     4801 ttgcttgctc ctttaggcgg caatttatgg cttattttat caggtataat ttcttgcgga
     4861 actttaggtg ctgcattgat tcctgaattc acaaaaatct ttacaagtcc taaagctaaa
     4921 cacactgaag aagtagttag agcttcacgc gaaggcggtg cgtctttgac aatcctttca
     4981 ggtcttgtat caggtaattt ttcaggattt tggattggaa tggttgttgt gatgctgatg
     5041 ggcggggctt atatgttcag cacatatatt cctgtcgaga tgatgattta tccgtcagta
     5101 tttgctttcg ggcttgttgc tttcggcttt ttgggaatgg gacctgttac aatcgcggtt
     5161 gacagttacg gaccagtaac agacaatgct cagtcagttt acgaattgtc tttgattgaa
     5221 gaaattccta acgttaagga agaaattgaa aaagacttcg ggttcacacc tgactttgaa
     5281 aatgcaaaac agtatctgga agaaaacga
//
LOCUS       DAOQAU010000078         4562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_24348_length_4562_cov_6.729532, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000078 DAOQAU010000000
VERSION     DAOQAU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24348_length_4562_cov_6.729532"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PKI94_08395"
     CDS             complement(<1..854)
                     /locus_tag="PKI94_08395"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNW26789.1"
                     /translation="MTIYEEQTKLLVQVLPEVAKEDCFALKGGTAINLFYRDLPRVSV
                     DIDLCYVNFEDRKTAITNIESAMERICQSVNNAGLRAEIKGTESKKIYCYNSSVSIKI
                     EPNYTIRGYAYEPEMMKICEKAEDDFGFAKIKVVSKAELYGGKICAALDRQHPRDLFD
                     IKELLDNDELDDAIIKGFIVLLLSAGRPLHEMLNPNILEQNDVFLSEFSGMTDKEFTY
                     QDHIETLKALIDIVNTEVKADYKDFLLDFVRLEHNFADIDIPNLDKLPAIKWKVKNLE
                     TLHTANKA"
     gene            complement(854..1618)
                     /locus_tag="PKI94_08400"
     CDS             complement(854..1618)
                     /locus_tag="PKI94_08400"
                     /inference="COORDINATES: protein motif:HMM:NF022898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNW26790.1"
                     /translation="MSTENKTNIKKIFTELPYNPVMTSKWLAEHGVYHDLQKKYERNG
                     WFKRIGIGAYAKPDEKLNIDSAMHALQEQLGLSIHVAAMTALNEKHGIMHNVPFNRKT
                     LLFGVRGEKLPAWFKNYFKDTYKLVLTDFLPKNLGMTTYNNGSFTTQIPTAERALLEL
                     IYLLPKDAGLKEVYHIMEMVMTLRPKKIQELLENCTSIKTKRIFLFLADKAQMPWFSE
                     LDLGKIDLGKGFREINKGGIVDYKYQIVINNPEDTN"
     gene            2292..3953
                     /locus_tag="PKI94_08405"
     CDS             2292..3953
                     /locus_tag="PKI94_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26791.1"
                     /translation="MGILKGYVNKENNLNRIYSREDVGKMSAAEYSKNEKAIEHQMKT
                     IGVPTNADLGNFSDVVYVSAYTRADGTEVGAYYRSKPDGIGGSSSTTGGGNGGIMDED
                     PHYPWEGKGDNESKNSMKDKEEKINPLNDLLNYAVTGNFNKDQFIDHLVRNVVPDDDA
                     QDPMGDDMGGKELLEMVKKLNKQIDPILTSVVDLLPQDSMVTDAVKSLVQYKINMGKQ
                     LEIMLDPEKRKEQQIEEMKQWVSNFADFTGQVVSGDFENIKWDEIGEKLKISQIAGMI
                     NPVAGVIATLAVDVAPKIVKLAEACNAGDKAEIIQEAIGAFTNCMGVVGQLKDAVGNK
                     LAEFSKEVDAKIYDEAAGTYDNLEKIQNTGYRYEKFVQAEMFDQANQEALKLDDLSKN
                     MMQGEATGFARDIKPSDLLTELKDSKNNLTLSAQIQKDVATNLPIQDLPEKYYRLSLN
                     NLSSINTIDNDNIKVKLNELGNTSIKDFIKIKHNVSDNVDVVLPKVDSKLSQEASKSI
                     ELKSLVKDNFEAIKNNSFKNKSIGLVKPPIFGPKLKIVFGNFLAA"
     gene            complement(3892..>4562)
                     /locus_tag="PKI94_08410"
     CDS             complement(3892..>4562)
                     /locus_tag="PKI94_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNW26792.1"
                     /translation="KAEGWRVNHKRVERIWREEGLRVPQKQKKRGRLYFNDGSCIRLR
                     PMHRNHVWSYDFVQDRLSNGKKIRILTVIDEYTRKCLKLKVGYSLKADAVLDALADLF
                     LTEGIPEYLRSDNGSEFVAKNIQSFLQQLHVTTAYITPGSPWENGFNERFNGILRDEL
                     LNGEMFYTIKEAEVMLEDYRRLYNEFRPHSSLNYAPPAPMAKVMPLKNCQKLSLTLDQ
                     RLGA"
BASE COUNT         1424 a          830 c          819 g         1489 t
ORIGIN      
        1 tttgccttgt ttgcggtatg taaggtttct aaatttttaa ccttccattt tatcgcaggg
       61 agtttatcaa ggtttggtat gtctatgtcg gcaaagttat gctccaaccg cacaaagtcc
      121 agtaaaaaat ctttgtaatc ggctttaact tctgtattaa caatgtctat aagtgctttt
      181 agtgtttcaa tgtggtcttg gtaagtaaat tccttgtcgg tcattccgct aaattcgctt
      241 aaaaacacgt cattttgttc caaaatgttt gggtttagca tttcatgcaa cggtctgcct
      301 gcacttagca ataaaactat aaagcccttt ataatagcat catccagctc atcattatca
      361 agcagttcct taatatcaaa caaatctctg gggtgttgcc tgtcaagtgc agcacaaatt
      421 tttccgccgt ataattcggc tttggataca acttttattt tggcaaaacc aaaatcatct
      481 tccgcctttt cgcaaatttt catcatttca ggctcgtaag cgtaacccct tattgtgtaa
      541 ttcggttcaa tttttatgct taccgaagag ttataacaat agattttctt gctttctgta
      601 cctttaattt ctgcccttaa tcctgcgtta tttacgcttt ggcaaatcct ttccatcgca
      661 ctttcaatat tggtaatggc ggtttttctg tcttcaaaat taacgtagca aaggtcaata
      721 tccacagata ctctcggcaa atccctataa aacaaattga tagccgttcc gcctttaagt
      781 gcaaagcaat cttctttcgc aacttccggc aacacttgta ccaacaattt tgtctgttct
      841 tcgtatatag tcactaattc gtatcctcgg gattatttat cactatttgg tatttgtaat
      901 caactattcc gcccttgttt atttccctaa aacctttgcc caaatctatt ttgcctaaat
      961 ccaattcgct aaaccagggc atttgtgcct tatccgctaa aaacaaaaat atacgctttg
     1021 tctttattga ggtgcagttt tccaacaact cttgtatttt tttcgggcgt aaagtcatta
     1081 ccatctccat tatatggtaa acttctttaa gccccgcatc ctttggtagc aagtatatta
     1141 attccagcaa agccctttct gctgtgggta tttgggtagt aaatgagccg ttattataag
     1201 ttgtcatgcc taaatttttc ggcaaaaagt cggttaatac cagtttgtat gtgtctttaa
     1261 aataattctt aaaccatgcc ggtagttttt ctcccctaac cccaaacaat aaagtttttc
     1321 ggttaaacgg tacattgtgc atgattccat gtttttcgtt taatgcagtc atggcggcta
     1381 catgtatgct caaacctaat tgttcttgta gggcatgcat ggcactgtct atgtttaatt
     1441 tttcatcggg ctttgcgtag gcacctatac ctatacgttt aaaccaaccg ttacgctcat
     1501 attttttttg caaatcatgg taaaccccat gttctgccag ccatttagat gtcataaccg
     1561 gattgtaagg taattcagta aatatcttct ttatatttgt tttgttttcg gtactcatgg
     1621 tacctatatt agcacatttt taaaataaaa gcaatatttt cgtaaatctt ttgcaataat
     1681 gattgacttg ttttatacac cttagttaaa atgaagctat agcttcattt taactaaggt
     1741 gtagcttgtt ttttgttgtg caaaaagagc ttatatattt gtttttaccc tatcaattcc
     1801 ttaacgctaa aaacaaaaat ttcatccatg gctaatttgt gaatcgtctc caacaaaggg
     1861 agttttattt tgctttttta acaaattgtt ttagcacaaa aatgcaaaac aatttgtgta
     1921 agtaatacaa aaatatcccc cagaggacac gaaccaaggt tgttttttcg ctcctgctct
     1981 aaaacaacgg ttggttaatc gacaagtcga taccctcatc tgcaaggctc gaacgtcctg
     2041 ttctcgcctg catctgagct gaagtccaac ctggggagtt ttttatttat aaggatttca
     2101 gccgatttgt atgatgccct tttttagtgc ttatacctaa taacacgacg atactatacg
     2161 gcacaagttt aattaaaagc ttaactgcca aatttgctta cgttattcaa gctaagtaaa
     2221 aatgcggact tgcgggcctt tttaaaaatt gttattctaa gaatagtgca attttttaga
     2281 aagggataac aatgggaatt ttgaaaggct acgttaataa agaaaacaac ttaaacagaa
     2341 tatattctag ggaagatgtc ggcaaaatgt ctgctgccga atacagcaag aatgaaaaag
     2401 caatcgagca tcaaatgaag acgattggtg ttccaacaaa tgcagatttg ggaaatttct
     2461 ccgatgttgt atatgtctcg gcatatacaa gagcagatgg aacagaagtg ggtgcatatt
     2521 atcgttcaaa acctgatggc ataggaggta gttcaagcac gacaggcggt ggcaacggcg
     2581 gtattatgga cgaagatcct cattatcctt gggaaggaaa aggcgataat gaaagtaaaa
     2641 attctatgaa agataaagaa gaaaaaatta atcctttgaa cgaccttttg aattatgcgg
     2701 taaccggaaa tttcaataaa gaccaattca ttgaccatct tgtgagaaat gttgttcctg
     2761 atgacgacgc tcaagatcct atgggtgatg atatgggtgg aaaagaatta ctagaaatgg
     2821 tcaaaaagct taacaaacaa attgacccga ttttgacttc tgttgtcgat cttttgcctc
     2881 aagattcaat ggtgaccgat gccgtaaaaa gtttggtgca atataaaata aatatgggta
     2941 aacaattaga aataatgctt gaccccgaaa aaagaaaaga acagcaaata gaagaaatga
     3001 aacaatgggt ttctaatttt gcagatttca caggacaagt ggttagcggc gattttgaaa
     3061 atattaagtg ggatgaaatt ggagaaaaat taaaaatttc tcaaattgca ggtatgatta
     3121 accctgttgc aggagttatt gcgacattag cggttgatgt tgcacctaaa atagttaaat
     3181 tggcagaagc ctgtaatgca ggagataaag ctgagataat acaagaagca atcggtgcct
     3241 ttacaaattg tatgggtgtt gtgggtcaat taaaagatgc cgtgggcaat aagcttgcgg
     3301 aattttcaaa agaagttgat gctaaaattt atgatgaagc ggcagggact tatgataatt
     3361 tagaaaaaat tcaaaacaca ggctacagat atgagaaatt tgtacaggct gaaatgtttg
     3421 accaagcgaa tcaagaagct ttaaaattag atgatttgtc aaaaaatatg atgcagggcg
     3481 aagctacagg ttttgcgaga gatattaaac cttcagattt attaacagaa ttaaaggatt
     3541 caaaaaataa ccttacattg tctgcgcaaa tacaaaaaga tgtggctact aatttaccaa
     3601 ttcaagattt acccgaaaaa tattataggc tttctctaaa taatttgagt tctattaaca
     3661 caattgacaa tgataatatc aaagttaaac ttaatgaact tggaaatact tcaatcaagg
     3721 attttattaa aataaaacat aatgtatcag ataatgtaga tgtcgtatta ccgaaggtgg
     3781 attcgaagct tagtcaagaa gcttcaaagt caattgaatt aaaatcactt gtaaaagata
     3841 actttgaagc aattaaaaat aatagtttca aaaataaatc tataggactt gtcaagcccc
     3901 caatctttgg tccaaagtta aagatagttt ttggcaattt tttagcggca taactttcgc
     3961 catcggagca ggtggagcgt agtttaggga gctatgcggt ctgaattcat tgtaaagtct
     4021 cctgtaatct tctagcatta cttctgcctc ttttatcgtg taaaacattt ctccgttcaa
     4081 taattcatct ctgagtatcc cgttaaaccg ctcattaaaa ccgttctccc aaggactacc
     4141 tggcgtgata tatgcagtgg tgacgtggag ttgttgcaaa aaactttgga tattttttgc
     4201 aacaaactcc gaaccgttat cactgcgaag atattctgga atgccttcag ttaagaataa
     4261 atccgcaagt gcatcaagaa cagcatcggc ttttaaactg taaccgactt tgagctttaa
     4321 acattttctt gtatattcat caattaccgt gagtatcctg attttctttc cattgctaag
     4381 cctatcctgc acaaaatcgt agctccaaac atgatttctg tgcataggtc gaagcctaat
     4441 acaagaacca tcattgaagt ataatcttcc tcgttttttc tgtttttgag ggactctaag
     4501 tccctcttct cgccaaattc tttcaactct tttgtgattt actcgccagc cctcagcttt
     4561 ca
//
LOCUS       DAOQAU010000079         4319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_26445_length_4319_cov_6.101079, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000079 DAOQAU010000000
VERSION     DAOQAU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26445_length_4319_cov_6.101079"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PKI94_08415"
     CDS             complement(<1..507)
                     /locus_tag="PKI94_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW26793.1"
                     /translation="MKKTITYKKNKLKIAFTLAEVLIVIGIIGMIADLTLPNLIKTFQ
                     KQQYVTALKKAYSEFNQALIMLANDMGCPGDLKCTGLFDGNSIFSANANQNLGKEIIK
                     YFKVTKNCETNASGKTTYGCFSNNILYDYNANPSQSGDYDDPQYYRFIILSGSSFRIL
                     NGNNNCSYN"
     gene            complement(586..1899)
                     /locus_tag="PKI94_08420"
     CDS             complement(586..1899)
                     /locus_tag="PKI94_08420"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNW26794.1"
                     /translation="MVEVKSLNAIVYNQERIDINDVIAPPYDVISDEYRDELYQRTPY
                     NIVKLILAKGSKDLSDPDNRYDEACKNFRQWLEKEVLIKLKKPCILYIIQKYTTEHGR
                     KIERKGFIARNRIEDFSTKKILPHEFTMDGPKEDRLNLTKKCKANFSQIFMVYSDPKK
                     QIENAVDFSQKPFIDVTDDNGVQNIVYKIEDKKFISLVENVMKDKTLLIADGHHRYET
                     AMNYRDICRQIPQQVRDDNYEYVMSYFTNLDDENLLVFPTHRIITKRIEPYVLLEKVK
                     KYFDVKEFIFDGKNKQKIKNEFLQAIEKENQKQISMGLYMKNVNKFYLLTLRENVNSI
                     LNEFAVPDVLKKLDLTVLHKVLITKEFGFTEDEQMAQEGIKYIKQESEAFDMIDSGKA
                     EASFIMAYPKIKDIKEISQAGFRMPQKSTYFYPKLLSGIVINPLE"
     gene            complement(1948..3789)
                     /locus_tag="PKI94_08425"
     CDS             complement(1948..3789)
                     /locus_tag="PKI94_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW26795.1"
                     /translation="MSSDTMKLKDTVSKDAFRYGYLLGRIYPYLKPVLGRAILGLLIA
                     IPLGLLDGVTAIALKPYLDNVINGSPNATFELLGKTFNSRIFWAMLIPPAIITFAVIQ
                     GGLKYINTYLTDWTGMKVSNSLKLDLFKKLTSLNSKFYDINSSGLVLIRFLNDPDAAS
                     RSIIDSVKTFITTIFGILGLVGVLLYTAWKLAIIGVTVLGLAMLPVAFIRKKIKSVSN
                     ASMVVGGGMTTNFNETFSGNKIIAAYNLQEQQNSAFESQINEQFNLSMTLTKRVGWLS
                     PIMYFVASCGIAIVMAYGNHLIMTGEITSGSFAAFVTSLLLLYKPVKGLGQTLAGLQS
                     TFVAMSRTFELFDLVPEIKNEPDAIELKGFEKQIEFKNVNFEYIEDTPVLKNLCLAVP
                     KNETIALVGNSGGGKSTVVNLIPRFYDVKSGSIEIDGVNIKNFDLVSLRNQISFVFQD
                     NFLFSGTIKENIIMGNKQASDEELQNVVKMAHLDEFIETLEKGIDTYVGERGATLSGG
                     QRQRVAIARAMLKNAPIVILDEATSALDNKSEAIVQKALDNLMKNKTVFVIAHRLSTI
                     KNADRIAVINEGELVELGTHDNLMRIENGKYKHLYEMQFKKVEQLNS"
     gene            3949..>4319
                     /locus_tag="PKI94_08430"
     CDS             3949..>4319
                     /locus_tag="PKI94_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26796.1"
                     /translation="MDKYCVQGENNKIILIEEGIERELGDCEVITGLNIEVKGNNNRI
                     KLELPTVFLNNTFIQIGNDGVDVTIKKNCRLKALFIRCCFGANQKLEIGEGTTFNSGS
                     IILDENAGCLIGNDCMFSSNI"
BASE COUNT         1343 a          737 c          726 g         1513 t
ORIGIN      
        1 attgtaagaa caattgttgt taccatttaa tattctaaaa gaagaaccac ttaaaattat
       61 aaatctataa tattgcgggt catcataatc ccctgactga gaagggtttg cattataatc
      121 atataaaata ttattagaaa aacagccata agtggttttt cccgaagcat ttgtttcaca
      181 attttttgtc actttaaaat attttataat ttctttccca aggttttggt ttgcatttgc
      241 tgaaaaaata ctgtttccat caaataatcc cgtacatttt aaatcacccg gacaccccat
      301 atcatttgct aacataatca aagcttgatt aaactctgag taagctttct ttaaagccgt
      361 aacatattgc tgtttttgaa aggtttttat taaatttggt aatgttaaat ccgcaatcat
      421 accgattata cctataacaa tcagaacctc tgctaaggtg aaagctattt ttagtttatt
      481 ttttttatat gtaatagttt ttttcataaa aataaaactt tctttaattc ttctaatttt
      541 aaaaagttta acatatcaaa gagttgtagt caattttata aatttttatt caagcgggtt
      601 aatcacaatt ccagagagca atttcgggta aaagtaagtc gatttttgag gcattctgaa
      661 ccctgcctga gaaatttctt ttatatcttt aattttagga tacgccataa taaaagatgc
      721 ttctgcttta ccagagtcaa tcatatcgaa ggcttcagat tcttgtttaa tgtattttat
      781 tccctcttga gccatttgtt catcttcggt gaacccgaat tcttttgtaa ttaagacttt
      841 atgcagcacc gtcaaatcaa gttttttcaa aacatcagga actgcaaact cattaagaat
      901 tgaatttacg ttttcgcgta acgtcaataa ataaaattta ttgacatttt tcatatacaa
      961 gcccattgaa atctgttttt gattttcttt ttcaatcgcc tgtaaaaatt catttttaat
     1021 tttttgttta ttttttccgt caaaaataaa ttcttttaca tcaaaatatt ttttaacttt
     1081 ttcaagcaaa acgtagggct caattctttt tgtaatgatt ctgtgggttg ggaaaacaag
     1141 cagattttca tcgtcaaggt ttgtgaaata gctcatgaca tattcataat tgtcatcccg
     1201 cacttgttgc gggatctgcc tgcaaatatc tctgtaattc atcgctgttt cgtatctgtg
     1261 gtgtccatct gctatcaaaa gagttttgtc tttcataaca ttttcaacaa gcgaaataaa
     1321 ttttttatcc tcgattttat aaacaatatt ttggactccg ttatcgtcag ttacatcaat
     1381 aaaaggtttt tgagaaaaat ctaccgcgtt ttcaatttgt tttttagggt cggaataaac
     1441 cataaaaatt tgagaaaaat ttgctttgca tttttttgtc aaattcagcc tgtcttcttt
     1501 aggcccatcc atagtaaatt cgtggggaag aatttttttg gtggaaaaat cttctattcg
     1561 gtttcttgcg ataaaacctt tgcgttcgat ttttctgcca tgttcggttg tgtatttttg
     1621 gataatgtaa agaatacaag gttttttaag ctttatcaaa acttcttttt caagccattg
     1681 gcggaagttt ttgcaggctt catcgtagcg gttatcaggg tcggacaaat ccttggagcc
     1741 ttttgccaga atcagtttta cgatattgta gggggttctt tggtacaatt catcgcggta
     1801 ttcgtctgaa ataacgtcat aaggcggtgc aataacatca tttatgtcaa ttctttcctg
     1861 attataaaca atcgcattta atgacttaac ctcaaccatc acttctcctc tttttagtca
     1921 acagcttttt aagaactgtt cgacttttca actattcaac tgttcaactt ttttaaactg
     1981 catttcataa aggtgtttgt acttaccgtt ttctattctc atcaagttat cgtgagttcc
     2041 taattccaca agttcgcctt cgttaataac ggcaattctg tcggcatttt taattgtaga
     2101 aagcctgtgt gctattacaa aaacggtttt atttttcatt aaattgtcca aagccttttg
     2161 tacaatagcc tctgacttgt tgtcaagtgc agaagtcgct tcatcaagaa ttacaattgg
     2221 cgcattttta agcatagccc gcgcgatagc aactctttgt ctttgaccgc cagaaagcgt
     2281 cgcaccgcgc tctcctacgt aagtgtcaat tcccttttca agggtttcga tgaattcatc
     2341 caagtgtgcc attttgacta cattttgtaa ttcctcgtcg cttgcctgct tatttcccat
     2401 aataatattt tctttgattg tgccggaaaa caggaaatta tcctgaaaaa caaacgaaat
     2461 ttgatttctc aaagatacaa ggtcgaaatt ttttatattt actccgtcaa tttctataga
     2521 gcccgattta acatcgtaaa accttggaat taagtttaca acggtacttt tgccaccacc
     2581 ggagttgcct acaagagcaa tggtttcatt tttaggaact gctaagcata gattttttaa
     2641 aacgggggta tcttcaatat attcaaaatt aacgttctta aattcgattt gtttttcaaa
     2701 gccttttaat tctattgcat ccggttcatt tttgatttca ggcacaaggt caaaaagttc
     2761 aaaagtcctg ctcatagcaa caaacgtaga ttgaagcccc gcaagagttt gccccaatcc
     2821 tttaaccggt ttgtaaagca ataaaagaga tgttacaaac gcggcaaaac ttccgcttgt
     2881 tatttcgcct gtcataatca ggtggtttcc gtatgccata actattgcaa ttccgcacga
     2941 tgccacaaaa tacataatcg gagaaagcca tccgacacgc tttgtaagag tcattgaaag
     3001 attaaattgt tcattaatct gactttcaaa agcagaattt tgctgttcct gaagattgta
     3061 tgcggcaatg attttatttc ccgaaaaagt ttcattaaag tttgtggtca ttccgccgcc
     3121 cactaccata gaagcatttg aaacggattt gatttttttt cttataaagg caacgggaag
     3181 cattgcaagc ccgagcaccg taaccccgat gattgcaagt ttccaggcgg tgtagagtaa
     3241 aactccgacc aagcccaaaa tgccgaaaat cgttgtaata aaagttttaa cgctgtcaat
     3301 tatacttctt gatgccgcgt cagggtcgtt taaaaatctt attaaaacaa gacccgatga
     3361 atttatgtcg taaaattttg agttaagcga agttaatttt ttgaataaat caagttttaa
     3421 agagtttgaa actttcatac cggtccagtc ggtaaggtag gtgtttatgt attttaatcc
     3481 gccctgaatt accgcaaaag tgattatggc aggaggaatg agcattgccc agaaaattct
     3541 gctgttaaaa gtttttccga gaagttcaaa agtggcgtta gggcttccgt ttataacatt
     3601 atcaagataa ggtttaagag caatggctgt tacaccgtca agaagaccta acgggattgc
     3661 tatcagtaag cctaaaattg ctcttcccaa aacaggttta agataagggt agattcttcc
     3721 caaaagatag ccgtacctga aggcatcttt ggaaaccgtg tcttttaatt tcattgtatc
     3781 cgaactcata attcctcaat tactgatgta catctgaata ctattttcac ataaaaaaaa
     3841 ctaaatttcc atataagcat attaattatt tataaagcaa ttattatggt aaaataaaaa
     3901 ttatgtagtg gttaataacg ttaaaaatat atttggaaaa ggagaaaaat ggataaatat
     3961 tgtgttcagg gggaaaataa taaaatcatt cttatcgaag aaggaattga aagagagctt
     4021 ggggactgtg aagttataac tgggttgaat attgaagtga agggaaacaa taatcgtata
     4081 aaattagaac tgcctacagt ttttttaaat aatactttta tacagatagg aaatgacgga
     4141 gttgacgtta ctattaaaaa aaactgtaga ttaaaagctt tgtttattcg ctgttgtttt
     4201 ggagcaaatc aaaaattgga aataggtgaa ggaacaactt ttaatagcgg atctattata
     4261 ttggatgaaa atgcggggtg tttgatagga aatgattgta tgttttcatc aaatataga
//
LOCUS       DAOQAU010000080         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_28483_length_4103_cov_6.249506, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000080 DAOQAU010000000
VERSION     DAOQAU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28483_length_4103_cov_6.249506"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PKI94_08435"
     CDS             complement(<1..438)
                     /locus_tag="PKI94_08435"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNW26797.1"
                     /translation="MKSVKNVKSLVITVAIIVFMLVAYFISASFFEPKAYNFMVRNFS
                     ANKVGSDEIALIIIDDKSLSQIRWPWKRNLYGKIFRYINEYTTAKVIGFDAIIATPDK
                     ENLKSDEEFYNSIKTIKNLVVGFVALNSPYENKTDGIKYDEKFK"
     gene            478..1089
                     /locus_tag="PKI94_08440"
     CDS             478..1089
                     /locus_tag="PKI94_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26798.1"
                     /translation="MQENLIKKISEQKIYPIIRCQDSKTAIDMANAMVEGGIKIIEIN
                     VENSSIYQAIHEVSKIATVSAGGVISSQQANKALDAGAQIISSPIFQMNMVKLSKDKE
                     VPLISCASTSNEAYMAWKTRVPLIKIFPAKALGGVLYIEDLLRPMPFLNIMPSGNISI
                     EEVPDYIKAGAVAVGVGRSFYENADFTEITRKSREILARLKEI"
     gene            1091..2029
                     /locus_tag="PKI94_08445"
     CDS             1091..2029
                     /locus_tag="PKI94_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNW26799.1"
                     /translation="MDEKLDIITIGESLIELSSDVSLSLAESLNKYYGGDTLTTAIAA
                     RRLGSKVGYITRVGCDYFKEFLLDSWQSEGLDISQIKLTGDFNGIYLLARPKNGPKEF
                     SYYRKKTAACKLSIEDISDNYIKNAKILYTSGIAQSLSLSAKEAIKKAYEIAKEKGIT
                     TAYDPNYTPLLMTPEESKELFDDVISDIDIIFLSAKNDAPHILEVDSCEKIIKNLWDL
                     GVNIVVVKSSHDKGYYTGYGGDIVFTPFFVKEIVDTTCSGDAFNGGFLHGISSGLSPF
                     EATKLASVVSGLQSQKIGAVKSVPYANEVYAEYEKI"
     gene            2016..3050
                     /gene="csaB"
                     /locus_tag="PKI94_08450"
     CDS             2016..3050
                     /gene="csaB"
                     /locus_tag="PKI94_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HNW26800.1"
                     /translation="MKKSKPPTVVISGYYGFDNFGDESILSVLINKLKELDAKIAVIT
                     KTPKKTSELHNVKTAWYICPRQIILSLLKCDVLVSGGGSLLQDVTSLKSLLYYLWVIN
                     TALFFKKKVIIFAQGIGPINSKLGQFLTKKALKKCSLITVRDEKSLFLLRGWGIDNVE
                     LVCDPLFDIEIPPSFRSDKVGVQLRDFKNLKEELLIKLAQQINENFSLKEIEIFSFQD
                     SIDLSVCKRFEAILKVMNPQIKTQIIYNQSSKELIDKISKLEYFIAMRFHANLIALKS
                     GVKTLAVSYDEKVEKLAKEAQIPCITINSTEDFKTLFEEIKNLNANKLKEFANSKHFD
                     WSKIEDILKV"
     gene            complement(3117..>4103)
                     /locus_tag="PKI94_08455"
     CDS             complement(3117..>4103)
                     /locus_tag="PKI94_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26801.1"
                     /translation="LSKRKKMRYDNYEIRKKKLDNLYSYKGPNFNYNGGHNCYMNSIF
                     SSYGNCQISNAQKSLNTWSNIMNGISIGTAIAGMFSAFKKTDDKPNPFKPAEPGDTTQ
                     KDVVTTTNDLEAALNTALETDNWQEVKNQLRTSKNTLSANQRTLANYDTQIQKAQADL
                     NITTGIIDDATTQNNQIDTDIERAETRKTQSIEAADTEFTTTEATINSQIAGLEQGVT
                     ALQGTPGAEAAIDRINQQIAQLRAQKTAAEQRCRDAKTTAEQTFTNTVTPLNETKTSN
                     ESKILEYTPVKETQTARLTRLSTEKTTLTTNNQALAQKITLAESRLQQYGRN"
BASE COUNT         1297 a          682 c          750 g         1374 t
ORIGIN      
        1 tttaaatttt tcatcatatt ttatgccgtc tgttttgttt tcataaggcg aattaagcgc
       61 aacaaaaccg actacaaggt tttttatagt tttaattgaa ttataaaatt cctcgtcgga
      121 cttcaaattt tctttatcgg gggtcgcaat tattgcatca aaaccgatta cttttgcggt
      181 tgtgtattca ttaatgtatc ggaaaatttt tccgtacaaa ttccttttcc acggccaacg
      241 aatttgtgaa agagatttgt cgtcaatgat aattaaagct atttcatcag atccgacttt
      301 atttgcagaa aaatttctta ccatgaaatt ataagcttta ggctcgaaaa aagacgccga
      361 aataaaataa gcaacaagca taaaaacaat tattgctaca gtaattacta aagacttgac
      421 gtttttcaca cttttcatga aaccatgata taataaattt tgaaaaaagg atacactatg
      481 caagaaaatt taattaaaaa aatatcagag caaaaaattt atccgataat aagatgtcaa
      541 gattcaaaaa ccgcaataga tatggcaaac gctatggtcg aaggcgggat aaagataatt
      601 gaaattaatg ttgaaaattc ttccatttat caggcaattc atgaagtttc taaaatcgca
      661 acggtttctg caggcggagt gatttcttct cagcaggcaa acaaggcttt ggatgcaggt
      721 gctcaaataa tttcatcccc tatttttcag atgaatatgg taaaactttc taaagacaag
      781 gaagttcctt tgatttcttg cgcttcgact tctaacgaag cttacatggc ttggaaaaca
      841 agagttcctt tgattaaaat ttttcctgca aaagctcttg gcggagtgct ttacatcgaa
      901 gatttgctta gacccatgcc gtttttaaat atcatgccgt cggggaatat ttcaattgaa
      961 gaagtgcccg attatataaa agcgggggcg gtcgcagtcg gggtaggcag aagtttttat
     1021 gaaaatgctg attttaccga aataacaaga aaatccagag aaattctcgc cagattaaag
     1081 gagatttaat atggatgaaa aattagatat aataaccatt ggtgaaagtt taatcgaatt
     1141 atcaagtgat gtaagcctta gtttggctga atctttaaat aaatattatg gcggagatac
     1201 tcttacaaca gccattgctg caagaagact cggctcaaag gtcggctata taactcgtgt
     1261 gggctgcgat tattttaaag aatttttgct tgacagttgg caatccgaag ggcttgatat
     1321 ttcacaaatc aagcttacgg gtgattttaa cgggatttat cttcttgcaa gaccaaaaaa
     1381 cgggcccaag gaattttctt attacagaaa aaagacagct gcttgtaagc tttccattga
     1441 agatatttca gacaattaca ttaaaaatgc aaaaatcctc tacacgtcag gaatagccca
     1501 atctctttcg ctttcggcaa aagaagccat taaaaaagct tatgaaatcg caaaagaaaa
     1561 aggtattaca accgcttatg atcccaatta tactcccctt ttaatgacac ctgaagaatc
     1621 aaaagagctt tttgatgatg taatatcgga tatagatata atcttcttga gcgctaaaaa
     1681 cgatgctccg catattttag aagtagattc atgtgaaaaa attattaaaa atctttggga
     1741 tttgggagta aatatagttg ttgttaaatc aagccacgat aaaggttatt acacaggcta
     1801 cggcggcgat atagttttca ccccgttctt tgtgaaagaa attgttgata cgacatgctc
     1861 gggtgatgct tttaacggcg gatttttaca cggaatatct tccggtcttt caccgtttga
     1921 agcaacaaaa cttgcttctg ttgtatcagg attgcaatca caaaaaatcg gtgctgtaaa
     1981 atcagtccct tatgcaaacg aggtttatgc tgaatatgaa aaaatctaag ccgccaactg
     2041 tggtaatttc aggatactac ggatttgata acttcggtga cgaatcaatt ttatcggttt
     2101 taataaacaa attaaaagaa ttagacgcaa aaatagccgt cattacaaaa acaccaaaaa
     2161 aaacttctga actgcataac gttaaaacag cctggtatat ctgcccgcgt cagataatct
     2221 tgtcattgct gaaatgcgat gtattagttt caggcggcgg aagcctgctt caggatgtta
     2281 caagtcttaa aagcctgctt tactacctct gggttataaa tacggcttta tttttcaaaa
     2341 aaaaagtcat aattttcgct caaggcatcg gtccgattaa ttcaaaattg ggacaattct
     2401 tgacaaaaaa ggcactcaaa aaatgttcgc tgattactgt tcgtgatgaa aaaagtttgt
     2461 ttttattaag gggttgggga atcgataatg ttgaattggt ttgtgatccg ctttttgaca
     2521 tagaaatacc gccgtctttc cgctccgata aagtgggtgt tcaactcagg gattttaaaa
     2581 atttaaaaga agaactgctt ataaaacttg cacagcaaat taatgaaaac ttttcgctaa
     2641 aagaaattga aattttttct tttcaagaca gcattgattt gagcgtttgc aaaagatttg
     2701 aggcaatttt aaaagtaatg aacccgcaga taaaaactca aataatttat aatcaaagct
     2761 ccaaagaact tattgataaa atttctaagc ttgaatattt tatagcaatg cgcttccacg
     2821 ccaatttgat tgcactaaaa tccggcgtca aaactcttgc cgtatcctac gacgaaaaag
     2881 tcgaaaaact tgctaaagaa gctcaaattc cttgtattac aataaacagt acagaagatt
     2941 ttaagactct ttttgaagaa ataaaaaacc ttaatgctaa taaattaaag gaatttgcaa
     3001 attcaaaaca ttttgattgg tcaaaaattg aagatatttt gaaggtttaa acaaaaagcg
     3061 gactaaaaaa gcgggcgagg ctgaaagcct cgcccgcttt aaaagttatt ttaaaattaa
     3121 tttcttccgt attgttgtaa tcttgactcg gcaagagtaa ttttttgagc caatgcttga
     3181 ttattcgttg ttaatgttgt tttttccgtg ctcaaacgag ttaatcttgc tgtttgtgtt
     3241 tcttttacag gggtatattc tagtatttta gactcatttg aagtttttgt ttcattaagc
     3301 ggcgttaccg tatttgtaaa agtttgctct gctgtagttt ttgcatcccg acatctttgt
     3361 tctgccgctg ttttttgcgc tcttaattga gctatttgct gatttattct gtctattgct
     3421 gcttcagccc caggagttcc ttgaagtgct gttacacctt gttcaagtcc tgcaatctga
     3481 gaatttattg ttgcttcggt agtcgtaaat tcagtatcgg cagcttcaat tgattgcgtt
     3541 tttcgggttt ccgctctctc aatatcagta tctatttgat tattttgagt tgtcgcatcg
     3601 tcaattattc ctgttgttat atttaagtca gcttgtgctt tttgaatttg agtatcataa
     3661 ttagccaaag ttctttgatt tgcggatagg gtgttctttg aggttcttaa ttgatttttt
     3721 acctcttgcc aattatctgt ttctaaggca gtatttaaag ctgcttcaag gtcatttgtt
     3781 gttgtaacaa catctttttg tgttgtatct ccaggctctg caggtttaaa tggatttggt
     3841 ttgtcgtcgg tttttttaaa tgcagaaaac attcctgcaa tcgctgttcc tatagaaata
     3901 ccgttcatta tatttgacca ggtgttcagc gatttttgag catttgagat ttggcaattt
     3961 ccgtatgaag agaaaatcga attcatatag cagttgtgac cgccattata attaaaatta
     4021 ggtcctttat aagaatagag gttatcaagt ttcttttttc ttatttcgta gttatcgtat
     4081 ctcatttttt tcctcttact taa
//
LOCUS       DAOQAU010000081         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_28858_length_4066_cov_5.433807, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000081 DAOQAU010000000
VERSION     DAOQAU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28858_length_4066_cov_5.433807"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PKI94_08460"
     CDS             complement(<1..835)
                     /locus_tag="PKI94_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNW26802.1"
                     /translation="MGKIKVADYLIQEINKLGIKEIFGLPGDYNFNILYAIEDNPKVK
                     WIGCTNELNAGYAADGYARINGYGALVTTYGVGELSAMNATAGSFAENIAVIHIVGAP
                     ATKFIKSNALIHHNFQHPNYYACQRAFSNVTEATAFLDENNAKEEIDRILSVFVEYNR
                     PVYISIPVDICKMEIENKPHIQRKKSDKKALASAIESAMRLINRCESPVILGDVLIKR
                     YKVRKEFAKLVEKSKMPVSNLLMGKGLLEADNKNFLGTFLSTYDNPNAYNGLRDSDCV
                     IS"
     gene            1028..1486
                     /gene="ndk"
                     /locus_tag="PKI94_08465"
     CDS             1028..1486
                     /gene="ndk"
                     /locus_tag="PKI94_08465"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNW26803.1"
                     /translation="MERTFVAIKPDGVKRGLIGKIISRIEDKGYKIVGLKMLHVTPEL
                     AAQHYAEHVGKPFYNGLIKFITSGPIIAMVLQGENVIESARKFMGKTNPDDAEGGSIR
                     GDFSQVMGINTIHGSDSKESAQREINLYFESNELCDNWKTMFELISDEKA"
     gene            1473..2594
                     /gene="tgt"
                     /locus_tag="PKI94_08470"
     CDS             1473..2594
                     /gene="tgt"
                     /locus_tag="PKI94_08470"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNW26804.1"
                     /translation="MKKHKFFEYELIHECKKTGARAGLLSTPHGDIETPIFMPVGTNS
                     TVKMLTNQHLEQTGAQIILANSYHLYLRAGTELIKSFGGIHGWMNWQKPILTDSGGFQ
                     VFSLSHLRKITEDGVKFRDPKDGKEHYISPEISMQIQEYIGADIIMAFDECAPYPCSH
                     SEAKVAMERTHRWLDRCFKAHKNPKQALFPIVQGAFYDDLREESAKIIASYDAVGYAI
                     GGVSVGEPTEMKNHIVEITAPLLPKDKPRYLMGVGTPEDLLDGIFRGIDMFDCVLPTR
                     NARHGSFFTYEGRKIIKNKEFETDSKPLDENCNCYACKNHSRAYIRHLYRCGEATAAI
                     LLSIHNTQFLIDFVKKARQAILKDKFDAFMQEYYEKISK"
     gene            complement(2595..2945)
                     /locus_tag="PKI94_08475"
     CDS             complement(2595..2945)
                     /locus_tag="PKI94_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26805.1"
                     /translation="MKINSINQRFLPQKTQNKNIYFNNKTAQDTVTLTMPRKDLNLII
                     NFINSQISQTNMALNGIEYDIIAKRHILSRLSDQYEIRGINSQITKLNKEHQRLENTF
                     NKLSKMLNALTPDK"
     gene            complement(2987..>4066)
                     /gene="lepA"
                     /locus_tag="PKI94_08480"
     CDS             complement(2987..>4066)
                     /gene="lepA"
                     /locus_tag="PKI94_08480"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNW26806.1"
                     /translation="YLTPNRVQVNSLKCGDVGYFAGSIKEITRFVGDTITHKSHPAKE
                     PLAGYKEALPMVFSGLYPVDNDEYHDLKEALERLKLNDSSITFEPESSGALGFGFRCG
                     FLGMLHMEIAQERLEREYNLSLVTTAPSVVYHVFKTNGEMVAIDNPANLPEPQYRDYI
                     EEPYVKVQIITPNEYVGTLMELAQSKRGIFMNMTYLDKTRADLEYQIPLSEVIIDFYD
                     QLKSRTKGYASMDYEFCDYKRSDLVKLDVMLAGEVVDALSVICHRDNAFYIGQKLTAK
                     LKDIIPRQLFEVPIQAAVGGRIIARTNIKAMRKNVLDKCYGGDISRKRKLLEKQKKGK
                     KRMKSVGRVEVPQEAFMAVLSLDED"
BASE COUNT         1199 a          777 c          791 g         1299 t
ORIGIN      
        1 cggaaatcac acagtcagaa tccctaagcc cgttgtaggc attggggtta tcgtaagtgg
       61 aaagaaatgt tcccagaaaa tttttgttgt cggcttctaa aagccctttt cccataagca
      121 gatttgatac aggcattttt gatttttcga caagttttgc aaattctttt ctgactttgt
      181 atctttttat caaaacgtct cccagtatta caggagattc gcagcgatta atcagcctca
      241 ttgcactttc tattgcggat gcaagagcct ttttatcgct ttttttgcgt tgtatatgcg
      301 gtttgttttc gatttccatt ttacaaatat caaccggaat tgaaatgtaa acgggtctgt
      361 tgtattcgac aaaaaccgac aaaattctgt caatttcttc ctttgcatta ttttcatcca
      421 aaaaagccgt tgcttcggtg acgtttgaaa acgctcgttg acaggcataa taattcgggt
      481 gttgaaaatt atggtggata agcgcgtttg attttatgaa ttttgtagca ggtgcgccta
      541 caatatgtat aactgcgatg ttttcggcaa agcttcctgc cgttgcgttc atcgcgctta
      601 actcgcctac cccgtaagtg gttacaagcg caccgtagcc gttaattcta gcgtagccgt
      661 ctgctgcgta acccgcgttc aattcatttg tgcagccaat ccattttact ttcgggttgt
      721 cttctattgc gtataaaatg ttgaaattat agtctcccgg aagcccgaag atttctttta
      781 ttccaagttt gtttatttcc tgtattaaat agtcggcgac tttaattttt cccatgccgt
      841 ttttctcccc aaaatgtata tgcaaataga ttttagcacg aattatatta tagtctttat
      901 ctttacaata tttaatttta aattcatccc ccagccttcc tcccttcaat cctcccgcgc
      961 gcaacaaagt ggacacaagt tgatttttgt ggtttaattt attcgaagaa attaaagagg
     1021 agactttatg gaaagaacat ttgttgcgat taagcctgac ggagttaaaa gagggttaat
     1081 cgggaaaatt atttcaagaa tagaagacaa aggatacaaa atcgtcggct taaaaatgct
     1141 ccatgtaacg cccgaattgg cggcacaaca ctacgccgag cacgtcggta aaccttttta
     1201 caacgggctt ataaaattta ttacaagcgg accgattatt gcaatggttt tacagggtga
     1261 aaacgtaatc gaaagcgcaa gaaaatttat gggaaaaacc aaccccgatg atgccgaagg
     1321 cggctctata agaggtgatt tttcgcaggt tatgggcata aatacaattc acggctctga
     1381 ttccaaagaa agtgcgcaaa gggaaattaa cctttatttt gaatcaaatg aactttgtga
     1441 taactggaaa acaatgtttg aactgatatc agatgaaaaa gcataaattt tttgaatacg
     1501 aattaataca cgaatgtaaa aagaccggcg cacgcgcggg tcttttaagc acgcctcatg
     1561 gcgacatcga aactccaatt ttcatgcccg tagggacaaa ttcaacggta aaaatgctca
     1621 caaatcagca tttagagcag acaggagcgc agattatcct tgcaaattct tatcatcttt
     1681 atttaagagc aggaacggaa ttgataaaaa gctttggcgg aattcacggt tggatgaact
     1741 ggcaaaagcc tattttaacc gattcgggcg ggtttcaggt tttttcgctc tcgcatttga
     1801 gaaaaataac agaagacggg gttaaattca gagaccctaa agacggcaaa gaacactaca
     1861 taagccctga aatttcaatg caaattcaag aatacatcgg agccgatatt attatggcgt
     1921 ttgatgaatg tgctccatat ccttgtagtc actcagaggc aaaagttgca atggagcgca
     1981 cccataggtg gttagataga tgttttaaag cgcacaaaaa tcctaaacag gcacttttcc
     2041 ccattgttca gggagctttt tacgatgatt tgagagaaga aagcgcaaaa ataattgcat
     2101 cttacgatgc tgtaggatat gctattggcg gagtttcggt gggtgagccg acagagatga
     2161 aaaatcatat tgtagaaatc accgctccgc ttttgccaaa agacaaaccg cgctatttaa
     2221 tgggtgtggg aactccagaa gatttgctcg atgggatttt tcgcgggatt gatatgtttg
     2281 actgcgttct gcccacaaga aacgcccgtc acggctcgtt ttttacttac gaaggcagaa
     2341 aaattatcaa aaataaagaa tttgaaactg attcaaaacc attggatgaa aactgcaatt
     2401 gctatgcctg taaaaaccat tccagagcat atataaggca tctttatcgc tgcggtgagg
     2461 cgactgctgc gattttgctt tcaattcata atacgcagtt tttaattgat tttgtcaaaa
     2521 aagcgcgaca agcaatttta aaagataagt ttgacgcttt tatgcaagaa tattatgaaa
     2581 aaataagcaa ataactactt atcaggcgtc agagcgttta acattttgct taatttatta
     2641 aaggtatttt ctaacctttg gtgttcttta ttcagttttg ttatctgaga attaatgccc
     2701 cttatttcgt attggtctga tagtctgctt aaaatatgcc ttttagcaat aatatcgtac
     2761 tcaatgccat ttaaagccat gttagtttgt gatatttgag aattaatgaa atttataatt
     2821 aaattcaaat cttttctcgg cattgttaaa gtaacagtat cttgagctgt tttattgtta
     2881 aaataaatat ttttgttttg cgttttttga ggtaaaaatc tttggtttat agaattaatt
     2941 ttcatattta aactcctttt tttgattata ttatcataaa aacaatttaa tcttcatcaa
     3001 ggctgagaac cgccatgaac gcttcttgcg gaacttctac cctgccgact gatttcattc
     3061 gttttttacc ttttttctgt ttttcaagga gtttgcgctt ccttgaaata tctccgccgt
     3121 aacacttgtc gagaacgttt ttgcgcatcg ctttaatatt tgttcttgca ataattcttc
     3181 cgcctacggc agcttggatg ggcacttcaa aaagctggcg cgggattatg tcttttaatt
     3241 tagcggttaa tttttgaccg atataaaaag cgttatctct atggcaaata acagagagcg
     3301 catctacaac ttcgcctgcg agcatcacat ctaatttaac caaatctgaa cgtttgtaat
     3361 cgcaaaattc gtaatccatg ctcgcataac cctttgtccg agattttaat tggtcataaa
     3421 agtcaataat aacttcactt aaagggattt gatactctaa atcggcgcgg gttttgtcca
     3481 aataagtcat attcataaaa attccgcgtt ttgattgcgc taattccata agagtgccaa
     3541 catattcatt tggtgtaatt atttgaactt ttacataagg ctcttcaata taatccctgt
     3601 attgaggttc gggcaggttt gcagggttgt cgattgcaac catttctccg tttgttttaa
     3661 aaacatgata aacaaccgaa ggggcggttg ttacaagcga caaattgtat tcgcgctcca
     3721 acctttcttg agcaatttcc atgtgaagca tgcccaaaaa accgcatctg aagccaaacc
     3781 ccaaggctcc tgaagattcg ggttcaaatg taatactgct gtcattgagt ttcagcctct
     3841 ccaaggcttc ttttaaatcg tggtattcgt cgttgtcaac aggataaagc cctgaaaaga
     3901 ccatgggaag tgcttcttta taacccgcaa gaggctcttt tgcagggtgg cttttatgag
     3961 tgattgtatc gcccacgaac cttgttattt cttttataga acctgcaaaa taaccgacat
     4021 caccgcattt aaggctattt acctgcaccc tgttaggggt aagata
//
LOCUS       DAOQAU010000082         3955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_30093_length_3955_cov_8.545385, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000082 DAOQAU010000000
VERSION     DAOQAU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30093_length_3955_cov_8.545385"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /gene="der"
                     /locus_tag="PKI94_08485"
     CDS             complement(<1..959)
                     /gene="der"
                     /locus_tag="PKI94_08485"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNW26807.1"
                     /translation="MKRKPIVAIVGRPNVGKSTFVNRLVGSRQSIVDDLPGVTRDRIY
                     FDVEWQNKYFTVIDTGGIIPGDDDEIMLSIFDQVQIACDEADKIIFLVDGKDGAQSAD
                     KDIANILRKNKKEVFLVVNKIDSVKENAMLADFYSLAMGEPMAISALHGSGGVGDLLE
                     IITENFEAQDEQVNDDTIKIAIVGRPNAGKSSIVNALLNEQRVIVSEVSGTTRDSIDS
                     KIKYKEKDFVLVDTAGIRKKAKVDWGVEKFAVDRAIHSIRDCDVAVLVIDATEGISDQ
                     DKKISAIITEAGKGIIITVNKWDLVENKQTNTMNQFDKKIAND"
     gene            1009..1875
                     /gene="hisG"
                     /locus_tag="PKI94_08490"
     CDS             1009..1875
                     /gene="hisG"
                     /locus_tag="PKI94_08490"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNW26808.1"
                     /translation="MEQLKIAIPNKGRLSEKIYELLSAAGLCFPSKSERTLQVTTKDG
                     KYSLIFVRTQDIPRFVEDGVADLGFTGFDIVKEEGASVNKIMDLDFGQCEMVVAVKEE
                     DKYKSASDLPIEIKVATSFPNITKEYFKKLGKKAKIIEVSGATEITPRLGLSNVVVDI
                     TSSGETLRSNKLKIIDKILTSTAVVISRKGLSEVQKKNTNTLLTALKSVLDAREKKYL
                     MAHVPKANLEKIKKFLPGLSSPTIMPLMNDEKNVVIHVVVDADKVYDSIGHLKELGGQ
                     GILIMTVDQMVK"
     gene            1943..3940
                     /locus_tag="PKI94_08495"
     CDS             1943..3940
                     /locus_tag="PKI94_08495"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNW26809.1"
                     /translation="MKKNFFVVLSIIILTIIVGRILSETYFFKHLELLSYDFRSRFNT
                     DNSFWSKDSNTADSDIVIVAIDDYSYKQLLKSSSKKAGQQEWENDVWKNVVNFIERGN
                     PKMVMFDMVFEKPEDKPWYDTSFAQTIKPYPNIILGTYLDDPLVKENNFTKKIELVKN
                     DFTPTAHPLDVSIDNKKLDDIITYTTNAPVNDIYAKYNNMGVINNVLDDDSVVRKNQP
                     LFKLVKEGKTYYMPSLAFAGFLKYMGEGGKIEVKKGKILYKNRVIPIDDEGIVNISWH
                     KLGRSYIYVPISRVLLSKGGQKDLKPEFFKDKMVIIGKTATGSTLDMNSIIDQSFTGP
                     EANAVALDNFINDTDPNNKQSRKFVHELSKPFEILMTVVICIVVALLGLVPQSAVIGF
                     LNGFASIIFYLSFSFWMFVNPTSRVWLPMVEPLYYLAMTAIVVFAFRLWGEMTRKASI
                     MNVFGKFVAPKVLASVLKDPNKLVLKNTKKRITILFCDVKNFSSLCEKYPPEQLVSNL
                     NELFKEVVNIIFANNGTVDKFIGDCIMAYWGDLTDSADEEFLAVKTALEIKKRINELK
                     ITNAQEGKIVFDVKIGINTGEAVLGLAGTDKIMSYTAMGDAVNTASRLETSCSTYKKD
                     ILISKSTYDAVQDKIIAFEIGKISVKGKEEQVEVFEPVDFA"
BASE COUNT         1272 a          720 c          768 g         1195 t
ORIGIN      
        1 atgtcgtttg cgattttttt gtcaaattgg ttcatggtat ttgtctgttt gttttcaacc
       61 aaatcccatt tattaacggt tattataatg ccttttccgg cttctgttat aatggctgaa
      121 attttcttgt cctggtcaga tatcccttct gtcgcatcaa ttaccaaaac cgccacatcg
      181 caatccctga ttgaatgaat ggctctatct actgcaaatt tttccacacc ccagtcaact
      241 ttggcctttt ttcttattcc tgcggtgtcc acaagtacaa aatctttttc tttgtacttg
      301 atttttgaat caatgctgtc gcgcgttgta ccagaaactt cagacactat aaccctttgc
      361 tcgtttaaaa gggcatttac gatagaagat tttcccgcat tggggcgtcc cacgatggca
      421 attttaatcg tatcgtcatt tacttgttca tcctgggctt caaaattctc agttataatt
      481 tcgagcaaat cgcccacccc gccgcttccg tgaagggctg aaattgccat aggctctccc
      541 attgcaagcg aataaaaatc ggctaacatg gcattttctt taacagaatc gattttattc
      601 accacaagaa aaacttcttt tttatttttt ctcaggatat ttgcaatgtc tttgtcggca
      661 ctttgcgcgc cgtcttttcc gtcaacaagg aagatgattt tgtctgcttc gtcgcaggca
      721 atttgcacct gatcaaaaat ggagagcata atctcgtcat cgtcgcccgg gataattccg
      781 cccgtgtcta ttacggtaaa atatttgttc tgccactcga catcaaaata aattctgtcg
      841 cgcgtaacgc cgggcaagtc atcgactatt gactgccttg agcctacaag gcgattgacg
      901 aaagtggatt ttcccacatt cggccgccct actattgcaa ctatcggttt tcttttcatt
      961 tttttattgt atcatgaact tgaaaaggaa aagtgcgagg gcaaaaaaat ggaacagctc
     1021 aaaattgcaa tccctaacaa aggaagattg tcagaaaaaa tttatgaact cttaagcgca
     1081 gcgggacttt gtttcccttc aaaaagcgaa agaacgcttc aggtcacaac taaagacggc
     1141 aaatattcgc tgatttttgt gcgtacacag gatattccgc gctttgtaga agacggcgtc
     1201 gcagacctcg gttttacagg atttgatatc gttaaagaag aaggcgcaag tgtaaataag
     1261 attatggatt tggatttcgg gcagtgcgaa atggtcgttg cggtaaaaga agaagacaaa
     1321 tataaatccg cttccgacct gcctattgag ataaaagtgg caacatcttt tccaaatatt
     1381 acaaaagaat attttaaaaa actgggcaaa aaagccaaaa ttatagaagt ttcaggtgca
     1441 acggaaataa ctccccgatt agggctttca aatgttgtcg tggatataac ttcctcgggc
     1501 gaaactctaa ggtcaaacaa actcaaaatt atcgataaaa ttttgacttc aactgcggtt
     1561 gtaatttcaa gaaaagggct atccgaagtt caaaagaaaa atacaaatac tcttttgact
     1621 gcgctcaaat cggttttgga cgcgcgcgag aaaaaatatt tgatggcaca tgttccaaag
     1681 gcaaatttgg aaaagataaa aaagtttttg ccggggcttt catcacctac gattatgccg
     1741 ttgatgaacg atgaaaaaaa tgttgtaata catgttgttg ttgatgccga taaggtctat
     1801 gacagtatag gacatcttaa ggaactgggc ggtcagggaa ttttaattat gaccgtcgat
     1861 caaatggtta aataacagta aatagtaaat agtaaatggt aaatagaaat tcacttatca
     1921 gcttttctgc tataatattt ttatgaaaaa gaattttttt gttgttttga gcataataat
     1981 tctgacgata attgtcggac ggatactgtc agaaacttat tttttcaaac accttgaatt
     2041 gctttcctac gattttcgtt caaggtttaa caccgataac agcttttgga gcaaagattc
     2101 aaatacggca gacagcgata ttgtgattgt tgcaatagac gattattcat acaaacaact
     2161 tttaaaaagc tcgtcaaaaa aagcaggtca gcaggaatgg gaaaatgatg tctggaaaaa
     2221 tgtcgtaaat tttattgagc gcggaaatcc taaaatggtg atgtttgaca tggtttttga
     2281 aaaacccgaa gacaaacctt ggtacgatac ttcgtttgca caaacaataa aaccttatcc
     2341 caacataatt ttagggacat acttggatga cccgcttgta aaagaaaaca atttcaccaa
     2401 aaaaatcgaa cttgtaaaaa atgattttac tccgacagcg catcctcttg atgttagcat
     2461 agacaacaaa aagcttgatg atattataac ttacacaaca aacgcgcctg ttaacgatat
     2521 ttacgccaaa tacaacaaca tgggcgttat aaacaatgta ctggatgacg attctgttgt
     2581 aagaaaaaat cagccgctgt ttaaactggt aaaagaaggg aaaacttact atatgccatc
     2641 tcttgccttt gctgggttct taaaatatat gggagaaggc ggcaaaatag aagttaaaaa
     2701 aggcaaaatt ttatataaaa acagagttat tcccatagat gacgaaggaa tagtaaatat
     2761 cagctggcat aaactaggca gaagttatat ttatgttccg atttcaaggg ttttactcag
     2821 caaagggggt caaaaagacc tcaagcctga gtttttcaaa gacaaaatgg taataatcgg
     2881 aaaaactgca acaggttcaa cccttgatat gaactcaata atagaccagt catttacagg
     2941 tcctgaagcc aatgcggttg ctctggacaa ttttataaac gatacagatc ctaacaataa
     3001 gcaatcaaga aaatttgttc atgagctctc caaacctttt gaaattttga tgacggtggt
     3061 tatttgtatt gttgtcgcgc ttttggggct tgttccgcag agtgctgtta taggattttt
     3121 aaacggattt gcttctataa ttttctacct gtctttttcg ttttggatgt ttgtaaatcc
     3181 tacttcaaga gtttggcttc ctatggttga gccgctttat tatcttgcaa tgacggcaat
     3241 tgttgttttt gcattcaggc tgtggggcga gatgacaaga aaagcatcta ttatgaatgt
     3301 ttttggtaaa ttcgtcgcgc ccaaagtcct tgcaagcgtt ttgaaagacc ctaataagct
     3361 tgtgcttaag aatacaaaaa agcgtataac aatcttgttt tgcgatgtaa aaaacttttc
     3421 aagcctttgt gaaaaatatc ctcccgaaca gttggtgagc aatttaaatg aactttttaa
     3481 agaagttgta aatataattt ttgcaaataa cggaacagta gataaattta tcggagattg
     3541 cattatggca tattggggcg acttgacgga ttctgccgac gaagaatttt tagccgtaaa
     3601 aaccgcactt gaaatcaaaa aaagaattaa cgaattaaaa attacaaacg ctcaggaagg
     3661 aaaaatcgtc tttgatgtaa aaatcggaat aaacacgggc gaggcggttt taggacttgc
     3721 cggaacagat aaaatcatga gctatacagc tatgggcgat gcggtaaata ccgcttcgcg
     3781 tcttgaaact tcctgctcaa cttacaaaaa agatatttta atttcaaaaa gtacttacga
     3841 tgcggttcaa gataaaatta tcgcttttga aatcggaaaa atctccgtca aaggcaaaga
     3901 agaacaggtt gaagtctttg aaccggttga ttttgcataa atttttaaaa ttata
//
LOCUS       DAOQAU010000083         3667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_33631_length_3667_cov_7.777409, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000083 DAOQAU010000000
VERSION     DAOQAU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33631_length_3667_cov_7.777409"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..737)
                     /gene="infC"
                     /locus_tag="PKI94_08500"
     CDS             complement(234..737)
                     /gene="infC"
                     /locus_tag="PKI94_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNW26810.1"
                     /translation="MLNNNIRAREVRLIDNEGENHGVIPTLQALKMAQEAGLDLVIIS
                     PNQEPPVAKILDHGKYKYEMEKKAKEAKKKQHVVEIKEVKIRYKIDTHDYQVRVKNIK
                     KFIAQGNKVKIVVMLRGREMQHAHLAFELAERFLTDLEGEPFMVEKKAQLEGRNVTLF
                     LAPQPLS"
     gene            complement(1026..2771)
                     /locus_tag="PKI94_08505"
     CDS             complement(1026..2771)
                     /locus_tag="PKI94_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26811.1"
                     /translation="MKISNQIQFRGTPLYLLKPFTTKTEQVKVKYSRVFGDYTPAYCD
                     KSSVSFCGTHDPSISKMGEIYSGLNGFSDQKRLFAPKYFAKPSVSKDLLSFIRGENPT
                     NPKTIETIMQMKTKAANFQAFFTNKDVKTVGDYKTDTLNFLSGIKENVKMINKTIDQV
                     GLSPQEAHLLYKTIAETSPILFTMGFGDAIPHAAQVSRKCMIETFKQGGSKEEILQAG
                     LIGWLHDPKFPLDFCWNNLATHPIIAGSLAYGILNKPENRREISLIFNGNKEKTDKFI
                     KGITETLFINNDSAFVMNKVILHKIKLPHAEEGMTDWIKSSDKIAQERLLAPSQGKKP
                     AEFSKDVRKQLQIVSMDTGISGISIDKLELILRDTYKDFPELKNKTSLEFLQELSEGL
                     INDKVLLNKIREKLEENHGIISIRVPGDNLFCHHQEVKNAKIAAKSLIIADPMLLSPY
                     KIVLEGTQNTALGRILSFCDSFNDNVKYVALEYKDTAKNWQKKLYESILKSADELTGK
                     STLSDFQKNNENTSLDSQIKELFKLIKNNENWGIYANISPADNEQRKELRTLARTIKK
                     YYDEAAETSSEMFGV"
     gene            3030..>3667
                     /locus_tag="PKI94_08510"
     CDS             3030..>3667
                     /locus_tag="PKI94_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNW26812.1"
                     /translation="MQDFNKFTNYSQEILSSAQNIMSSNKNFELQPEHILMAMIKDVE
                     GVVGDYLKELRLLQQSFIDKVVKAIKDYPTVSGPINTGQLYLSQKANELLDIADAQAD
                     ELKDEFISIEHILIAMTKIEHNVQQLLNEFGVNTNSVLKAMKNIRGNKKVDNKDAEEM
                     YKALEKYSTDLTDLARKGKLDPVIGRDEEVRRIIQVLNRRTKNNPCLIGEPGV"
BASE COUNT         1050 a          648 c          634 g         1335 t
ORIGIN      
        1 gctattgcgg catattctat ttaattgttg aaatcagttg gtagagcaag ctcttttcaa
       61 tctgacttcg gtccggctta gcctcaccga ggctattgcg acatattgta tttgattttc
      121 atatttttgg attgcttcgg gggcaacccc tcacaatgac aaacttttat gatgccaaaa
      181 taaattcagc atacctttat cctatcaaaa aaaacctttc agtcaagcca atcttaagat
      241 aaaggctgag gtgcaaggaa caatgttacg tttctgcctt ccagttgagc ttttttctca
      301 accataaaag gttcaccttc taagtctgtt aaaaaccgct ctgccaattc aaaggcaaga
      361 tgtgcgtgtt gcatttcccg ccctctgagc attacaacta ttttaacttt attcccttgc
      421 gcgataaatt tctttatatt tttaactctg acttgatagt catgagtgtc aattttatat
      481 cttattttta cttctttaat ttcgactaca tgttgttttt tcttagcttc ttttgccttt
      541 ttttccattt catatttata tttgccgtgg tctaaaattt ttgcaacggg aggctcttgg
      601 tttggactta ttattaccaa atccagtccg gcttcttgtg ccatttttaa tgcttgcaaa
      661 gtcggtatta caccgtgatt ttccccttca ttatctatta atctcacttc tcttgcgcgt
      721 atattgttgt ttaacaatgc gtttttccct gatttatcct cgtgtgctat ggctgtttct
      781 cctttatcta atgttttaca aagtaaataa taccataatt tttagttttt aatcttcttt
      841 gtaacaaagt gttgcgaata attagcgggg cgggaatccg cctctctttt tatgtgtaca
      901 agtcactatt gcaaaattaa agcaaagagt aggctatatt cgtaagccgt gacgccattt
      961 ttcaagtttc gagcatagct gtcaacggag aaagaaggca aaatcccgcc ctattgatta
     1021 cttatttaca ccccgaacat ttctgaactg gtttccgcgg cttcatcgta atattttttg
     1081 atagtacgag ccaaagtcct aagttctttt ctttgttcat tgtcagcggg gcttatgttt
     1141 gcgtatattc cccaattttc attattttta atcaatttga ataattcttt aatctgactg
     1201 tcaagacttg tgttttcatt atttttttga aagtcgctta atgtagattt tccggtcagt
     1261 tcatcagcag atttaagtat gctttcatac aacttttttt gccaattttt tgccgtatct
     1321 ttatattcca acgccacata ttttacattg tcgttaaaag aatcgcagaa agacaagatt
     1381 ctgcccaaag cagtattttg ggtgccttct aaaactattt tgtaagggct tagcagcata
     1441 ggatctgcaa taataagact tttagcggca atttttgcgt ttttaacttc ctggtggtga
     1501 caaaacagat tatctccggg aactctaata cttattattc cgtgattttc ttccaatttt
     1561 tctctgattt tgttcaaaag tactttatcg tttataagcc cttctgataa ttcttgaaga
     1621 aattctaaag aagttttatt tttaagttcg ggaaagtctt tataggtatc tcttaaaatt
     1681 aattcaagtt tatctatact tattccgctt attccggtat ccattgaaac tatttgtaat
     1741 tgttttctta cgtctttaga aaattctgcc ggttttttgc cttgggatgg tgccaaaagt
     1801 ctttcttgag cgattttgtc tgaactttta atccaatcgg tcatgccttc ttctgcatga
     1861 ggaagtttaa ttttatgcaa tattacttta ttcattacaa aggctgagtc attgtttata
     1921 aaaagtgtct ctgtaatgcc tttaatgaac ttgtctgttt tttctttgtt gccgttaaag
     1981 ataagtgata tttctcttct gttttccggt ttatttaaga ttccgtaagc caaagaacct
     2041 gcaataatag ggtgcgtagc aagattgttc cagcaaaaat ccagcggaaa cttagggtcg
     2101 tgaagccagc ctattaatcc tgcctgaagt atttcttctt tagagccgcc ttgcttaaaa
     2161 gtttctatca tgcactttct tgatacttgt gccgcatgag ggatggcgtc tccaaaaccc
     2221 atagtgaata aaataggtga ggtttcagca atggttttat acaatagatg tgcttcttgc
     2281 ggtgatagcc ctacctggtc aatggtttta tttatcattt taacattttc tttgatgccg
     2341 gataaaaaat ttagcgtatc tgttttataa tctccaactg ttttgacatc tttgttggta
     2401 aaaaacgctt gaaaatttgc tgctttggtt ttcatttgca ttattgtttc aatcgttttt
     2461 ggatttgtag ggttttcacc tctgataaaa ctcaaaaggt cttttgatac agacggtttt
     2521 gcaaaatatt ttggagcaaa aagtcttttt tggtcactaa aaccgtttaa tcctgaataa
     2581 atttcgccca ttttacttat gcttggatcg tgagtgccgc aaaaacttac actcgattta
     2641 tcacaataag caggagtgta atcaccaaag acacgagaat atttaacttt gacttgttca
     2701 gtcttagttg taaatggttt aagtaaatat aggggtgtgc ctctaaactg aatttgattg
     2761 gagattttca tttttatttt cttctttact tggttttaca ttatattcta actcgtaaaa
     2821 taaaataagt tgctatattt acaatttatt attaaaattt gtttacattt cttgcggctg
     2881 aaaatcattg attttcagcc gcactttttt cagaattatt aataaaaaat gaagtttaaa
     2941 tgctttttta aaaaaaagcc ttatattaaa aaatatagtc tcattaaaac tttagtaagg
     3001 agcgggaatt tatctcgtgt tgtggcaaaa tgcaggattt caacaaattt accaattatt
     3061 ctcaagaaat tttatcttcg gctcaaaaca taatgagttc aaacaaaaac tttgaacttc
     3121 agcctgaaca cattttaatg gctatgataa aggacgtaga aggtgttgta ggcgattatc
     3181 ttaaagagtt gagacttttg cagcagtcct tcattgataa agtcgtaaaa gcgattaaag
     3241 actatccgac tgtttccggg cctataaata caggtcaatt atacctttcg caaaaggcaa
     3301 acgagctttt ggatatcgcc gatgctcagg cagacgaact taaagacgaa ttcataagta
     3361 ttgaacacat cttaatcgcg atgacaaaaa tcgaacacaa tgttcagcaa ttgttaaatg
     3421 aattcggtgt aaatacaaat tcggtattaa aagctatgaa aaacataaga ggtaataaaa
     3481 aagtggacaa taaagacgca gaagaaatgt acaaggcact tgaaaagtat tcgactgatt
     3541 taaccgattt ggcgcgcaag ggaaaacttg accctgtaat cggcagagac gaagaagtca
     3601 gaaggattat tcaggtctta aaccgccgta caaaaaataa cccgtgttta atcggagagc
     3661 cgggggt
//
LOCUS       DAOQAU010000084         2987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_45498_length_2987_cov_7.914393, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000084 DAOQAU010000000
VERSION     DAOQAU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45498_length_2987_cov_7.914393"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..2683)
                     /locus_tag="PKI94_08515"
     CDS             complement(473..2683)
                     /locus_tag="PKI94_08515"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNW26813.1"
                     /translation="MAEKPIFTELKDKLTEQNRVPEDIEKIEQAFLFAQKLHEGQYRI
                     SEEPYIVHPIEVAKILIDLKMDTNTIIAALLHDILEDTETPPEEIENLFSTDVLNLVQ
                     GVTKLGKLQFKSTEERQAENFRRMFIAMASDIRIIFLKLADRLHNMRTLNFMAAGKQQ
                     KIAQETLDIFAPLANRLGIGKIKAELEDLSLRYLHPDKYYEIAQLVSQKKAERELTVN
                     LLIDKISLDVKNAKINASITGRAKHYYSIYSKMERLNIAFHDLYDITAVRVIVDTERE
                     CYEVLGLIHSQFKPIPGRFKDYIAMPKGNLYQSLHTSVIGPRSKPLEVQIRTWEMHEV
                     AEYGIAAHWRYKEKGSEKAINNSDVQFSWMRKLVEYEKDTEDAKEYVDGVKLDIFSDQ
                     VFVFTPRGDVFDLPQNATPVDFAYRIHSDVGHKTVGSLVNGRIAQLDTKLKNGDIVEI
                     LTSKTPAPRLDWLNFVVTKQAASKIKQWFKKNNREDHINLGKANLEHELTKAVFDEYT
                     KQDEFEKVAKQMNYQSAEDLFAALGYGETTVNKVVNKLKKPTVKSEGQAFSQSGKSRK
                     SDKDILGLEGLMYSLAKCCSPVPGEPIVGVVTRSKGVSVHRLDCKSLDTIPPERFMDI
                     VWAKENINKTYITTIRVEAQDKLGLLKDIITQVSDNNTNIVFANVKSKNNKLGIIELG
                     IELDNVDTLKRVIVSLQALPEVYSVKRIQTSSNQTGVAAVKKNTKPQKKKHVKH"
     gene            2739..>2987
                     /locus_tag="PKI94_08520"
     CDS             2739..>2987
                     /locus_tag="PKI94_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNW26814.1"
                     /translation="MKNIFEKNNKRLKLQIKVIPNSSKNEIIFEQNFVKIKLTAPPVE
                     NKANKALIEFLAKKLKTAKSNIQIIKGETSKEKTLLISS"
BASE COUNT          872 a          592 c          505 g         1018 t
ORIGIN      
        1 gtttgacaaa atttgttaaa atattttaat aaaactttac tcgtacctct gctcgtctgg
       61 tgtcttggat tattcgggtg agcatagtcc ttcattccgt gacgccattt tgcactaaat
      121 cctagatttc ggcaaaagca ggcattgctc ctgtggtact cggcttcgcc tgcgttccta
      181 cgtcgctatc gatcggtttc ggctctccaa agtcacgcat ttctgcgtgc catttcgagc
      241 cttcacacac ggctcacgca taaaaaaata gctgcttata gcagctagta cttcataatc
      301 ttgggaggag atttggggat agttgtacat gcatgataat tcactgaatt aaaaaatgtt
      361 actatttaag tcaaaaaatt aataaaaatt tacaatctgt ttaaatttta attatttttt
      421 gtatgtaaat ttaaaaagag agcatgaatt catgacaaaa tttatacaaa aattaatgtt
      481 tgacgtgttt tttcttttgg ggcttagtgt ttttcttaac ggcagcaact cctgtttgat
      541 ttgacgaagt ttgaattctt tttacgctgt aaacttcagg caatgcctgc aagcttacta
      601 taactctttt taatgtgtcc acattgtcca attctattcc caattctata ataccgagtt
      661 tattattttt tgacttaaca ttggcaaaaa caatattggt attgttatcc gaaacttgag
      721 tgataatatc ttttaaaagc cccaatttat cctgagcttc tactctgata gttgttatat
      781 aagtcttatt aatattttct tttgcccaaa ctatatccat aaatctttcg ggcggaatag
      841 tgtctaaaga tttgcagtcg agcctgtgaa cagaaactcc ctttgatcgt gtaaccacac
      901 ctacaatggg ttctcccggg acaggcgaac agcattttgc aagagaatac attaaaccct
      961 caagtcccaa aatatcctta tcggattttc tgcttttgcc gctttgcgaa aaagcttgtc
     1021 cttcagattt aacagtcggt tttttaagtt tgtttacaac tttatttacc gttgtttcac
     1081 catagcccaa agcggcaaat aggtcttctg ccgattggta attcatttgt tttgcaactt
     1141 tttcaaactc atcctgtttg gtgtattcgt caaaaacggc ttttgtaagc tcatgttcaa
     1201 ggtttgcttt gcctaaatta atatggtctt cgcggttatt tttcttaaac cattgcttaa
     1261 tttttgaagc cgcttgcttt gttaccacaa aattaagcca gtcaagacga ggtgcgggtg
     1321 tttttgaagt taaaatctca actatatccc cgtttttgag ctttgtgtca agctgggcta
     1381 ttctgccgtt tacaagtgag ccgaccgttt tgtgccccac atccgagtga attctatatg
     1441 caaaatcgac aggcgtggca ttttgaggaa ggtcaaaaac atcgccccgc ggcgtgaaca
     1501 caaaaacctg atcagaaaaa atatccaact taaccccgtc aacatactct tttgcatctt
     1561 cggtatcttt ttcatactcg accagttttc tcatccaaga aaattgaaca tctgaattgt
     1621 taatcgcttt ttcagaacct ttttctttat aacgccagtg ggcggcaata ccgtattcgg
     1681 cgacttcgtg catttcccaa gttcttatct gaacctccaa aggctttgag cgcggaccta
     1741 ttacagaagt gtgcaaggat tgatacaagt tgccctttgg cattgcaata taatctttaa
     1801 atcttcccgg aatcggctta aattgcgagt gaattaatcc caaaacttca taacattcac
     1861 gctctgtatc aacaataacc cttaccgcgg tgatatcata caagtcgtga aaagctatgt
     1921 taagtctttc cattttgctg taaatgctgt aataatgctt ggctctgccg gtaatagacg
     1981 cgtttatttt tgcattttta acatccaaag aaattttatc tattaaaaga tttaccgtaa
     2041 gttctctttc ggcttttttc tgggaaacaa gctgggctat ttcgtaatac ttatccggat
     2101 gcaaataacg cagggataaa tcctcaagtt ctgcttttat ttttccgata cccaaacgat
     2161 ttgcaagcgg agcaaaaata tctaaagttt cttgcgctat cttttgctgc ttgcctgctg
     2221 ccataaaatt aagagtgcgc atattgtgaa gtctgtcggc aagtttgaga aaaattattc
     2281 ttatatcgct tgccattgcg ataaacattc ttctaaaatt ttccgcctga cgctcttcgg
     2341 ttgacttaaa ctgaagcttc cctaactttg tgaccccctg aacaagattt aaaacatctg
     2401 ttgaaaacaa attctctatc tcttccggcg gagtttcagt gtcttctaaa atatcatgca
     2461 aaagtgctgc aattattgtg tttgtatcca tttttaagtc aatgagaatt tttgcgacct
     2521 caataggatg cacaatgtat ggctcttcgg agattctgta ctgcccttca tgaagctttt
     2581 gtgcaaacaa aaaagcctgt tcaatctttt caatatcttc aggtactctg ttttgctctg
     2641 tcagcttatc tttcaactct gtaaaaatgg gtttttctgc catggtataa ttattatact
     2701 ctttttcttc acatttgcat agtaggcagg agatactaat gaaaaacata tttgaaaaaa
     2761 ataataaaag attaaaatta caaataaaag ttattccaaa ttcttcaaaa aacgaaatta
     2821 tttttgagca aaattttgtt aaaattaaac ttaccgcacc gcctgttgaa aataaggcaa
     2881 acaaagccct aatcgagttt ttggcaaaaa aattaaaaac tgcaaaaagc aatatccaaa
     2941 taataaaagg tgaaacgagc aaagaaaaaa cattgcttat ttcatct
//
LOCUS       DAOQAU010000085         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_47227_length_2914_cov_9.968171, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000085 DAOQAU010000000
VERSION     DAOQAU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47227_length_2914_cov_9.968171"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKI94_08525"
     CDS             complement(<1..737)
                     /locus_tag="PKI94_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNW26815.1"
                     /translation="MQNEITSLNNDLVKQTLKLHQKKYRDEEKKFLLEGFKAIEEAFL
                     AKIEFEYVFVEKNKAGKYGFIINPSPALRAPSPARGEGKIIITNEAVLKKISTTDSPP
                     EAVAVAKQKIYTLKDIKSTKKVVLLENIKDLGNLGTIIRTAKAFNQDAIILFGQTADI
                     YNPKCVRSAVGNLWKIPIVQIKDFEILKENFNGFDKAAALPNSKGSILLKDFTAKPPY
                     LIMFGSESEGLSKELIDFADKKLTIEMS"
     gene            complement(727..2394)
                     /locus_tag="PKI94_08530"
     CDS             complement(727..2394)
                     /locus_tag="PKI94_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNW26816.1"
                     /translation="MQKKIIEKVRQFLKNENCAKESYDYLLVNSTNEFLVEYNELEKN
                     SRYLITGFSGSAGEALISQKDLFLFVDSRYHLQAELEVDKNLVKVVKMKSGESFSQLI
                     CKKIKPRKTLAITAKKVSQATLEILENTLKSKNIKIKLLDFDPIEKKIKIKEPQKCTK
                     VDKKIAGLSTDEKFKKVSKNLKKNEAILTTNLEEISYLTNLRDLSQNYSSKLKAAKCV
                     VTKDFAILFTDYKVGKIGENFKVKKLCEFENFIKNSDQINTVFVDKRFINVYDYNLLG
                     KKAKSLKQNPIILMKSVKTKQEIEHYKICFKKADKALFALRDYIEKNENISEYDISKK
                     LEEEFLKNGAKSLSFKSIIAKDANSAQAHYSKNSKKEIVKEGSLILIDCGAYFEGGLA
                     TDCTRVFVKGTPSKLQKQVYTAVLKAFLKAFSLKITDKTTGFAVDKPVRKLLEKIAPK
                     GFCFSHSLGHGIGINVHEAPPKLAFSPVAKTPLKQNMCFTIEPGLYKKGAFGVRLENS
                     CFLDKENGKLIIKSLSNMCFEKKLIDFNSLTKTEKIILSRFEAKDAK"
     gene            complement(2398..2682)
                     /locus_tag="PKI94_08535"
     CDS             complement(2398..2682)
                     /locus_tag="PKI94_08535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW26817.1"
                     /translation="MTQEVKASHLLVETEQEAISLREEILSGTAFEDAAAEHSLCPSG
                     ANGGDLGFFGRGQMVFEFEKAAFDLKVGELSEPVKTNFGWHLILVTDKQD"
BASE COUNT          802 a          517 c          468 g         1127 t
ORIGIN      
        1 gacatttcta ttgttaattt tttatcggca aagtcgatta attctttgct taaaccctca
       61 gactctgagc cgaacatgat taaataaggc ggctttgcag taaaatcttt tagcaaaata
      121 cttccttttg aattgggcaa agccgccgct ttgtcaaagc cgttaaaatt ttctttcaat
      181 atttcaaaat cttttatttg tacaataggg attttccaca aattcccaac tgccgaacgc
      241 acgcattttg ggttataaat atctgccgtt tgtccaaaaa gtattatcgc atcttgatta
      301 aatgcttttg ctgttcttat tattgtgccc aaattcccta aatcttttat attttcaagc
      361 agaacaactt tttttgtaga ttttatgtct tttaaagtat aaattttttg ttttgcaacc
      421 gccaccgctt caggtgggga gtctgttgta gaaatttttt tcaaaaccgc ttcgtttgtg
      481 ataataattt tcccctcgcc ccttgcggga gagggtgccc gaagggcggg tgaggggtta
      541 atgataaaac cgtattttcc ggctttattt ttttccacaa aaacgtattc aaattcaatt
      601 tttgctaaaa aagcttcttc tattgcttta aagccttcca acagaaactt tttttcttca
      661 tcgcggtatt ttttttgatg aagcttcagg gtttgtttta ccaagtcgtt attcaagctt
      721 gtaatttcat tttgcatctt ttgcctcaaa tctgctaagt ataatttttt cggtttttgt
      781 aagcgaatta aaatcaatca attttttttc aaaacacata tttgaaaggg attttataat
      841 taatttacca ttttctttgt ctaaaaagca agaattttcc aaacgcacac caaaagctcc
      901 ttttttataa agccccggct ctatcgtaaa acacatgttt tgttttaagg gcgtttttgc
      961 cacaggcgaa aacgcgagct ttggcggtgc ttcgtgaaca ttaattccta tgccgtgtcc
     1021 cagcgaatga gaaaagcaaa acccctttgg cgcaattttt tcaagcaatt tccttacagg
     1081 tttgtcaacg gcaaaacctg ttgttttatc cgttatcttc aagctgaaag cctttaaaaa
     1141 agcttttagg actgccgtat agacttgttt ttgaagtttt gaaggcgtgc cttttacaaa
     1201 aacccttgtg cagtctgttg ccaagccgcc ttcaaagtat gctccgcaat cgattaagat
     1261 aagcgagcct tcttttacga tttctttttt tgaatttttt gaatagtgtg cttgtgccga
     1321 attggcatct tttgctatga ttgatttaaa gctcaaggat tttgcgccgt ttttcaaaaa
     1381 ttcttcttcc aatttttttg aaatatcgta ttcggaaata ttttcatttt tttctatata
     1441 atctctcagt gcaaataaag ctttgtctgc ctttttaaag cagattttgt agtgttctat
     1501 ttcttgtttt gttttaactg atttcatcag gataatcgga ttttgcttta gagatttagc
     1561 ttttttgcct aaaagattgt aatcgtaaac atttataaat cttttgtcaa caaaaacagt
     1621 attaatttgg tctgaatttt ttataaaatt ttcaaattcg cataattttt taactttaaa
     1681 attttcacca atttttccca ctttataatc ggtaaaaagt atggcaaaat cttttgtaac
     1741 aacacatttg gcagctttga gtttactgga ataattttgt gataaatctc tcaaatttgt
     1801 aaggtaagaa atttcttcca aatttgttgt caagattgct tcattttttt tgagattttt
     1861 tgagactttt ttaaattttt catctgttga caatcctgca atttttttat caacctttgt
     1921 acatttttgg ggttctttta ttttaatttt tttctcaatc gggtcaaaat ccaaaagttt
     1981 aatttttatg tttttgcttt tgagtgtatt ttctaaaatt tctaatgtcg cctgagaaac
     2041 tttttttgcg gttattgcaa gtgttttgcg gggttttatt tttttgcaga taagctgaga
     2101 aaaactttct cctgatttca tttttacgac ttttacaaga tttttgtcaa cttcaagttc
     2161 tgcctgaagg tgatagcggc tgtccacaaa aaggaataaa tccttttgag aaataagtgc
     2221 ttctccggca gagccggaga agcccgtaat caaatatctc gaattttttt ccagttcatt
     2281 atattccacc aaaaattcat ttgtggaatt tacaagaaga taatcataac tttctttggc
     2341 acaattttca tttttgagaa attgccgtac tttttcaatt attttttttt gcatttttta
     2401 atcttgttta tcggttacca aaatcaagtg ccagccgaaa tttgttttaa ccggctcgga
     2461 aagttcaccc acttttaagt caaatgctgc tttttcaaat tcaaaaacca tttgtccgcg
     2521 tccgaaaaac cctaaatccc cgccgtttgc gcctgatgga caaagcgaat gttcggctgc
     2581 tgcatcttca aatgccgttc cggaaagtat ttcttctcgc aaactgatgg cttcctgctc
     2641 tgtttcaacc agaaggtggc ttgcttttac ttcttgtgtc atactaactc ctttaaaacc
     2701 ttaactttaa cattgtatca aaaaaataag tttacattaa gctttatttt aactaaactt
     2761 gaattatgaa attatacgaa caatcaggtg ttaaccttgc cgaagcccaa aaactcaatg
     2821 acaggttaag taaaaattta gggataaaat cttttgcggg aattgttgat ttgtctgagg
     2881 aattttcgct tgtaacctgt tgcgatggaa tcgg
//
LOCUS       DAOQAU010000086         2895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_47708_length_2895_cov_7.095423, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000086 DAOQAU010000000
VERSION     DAOQAU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47708_length_2895_cov_7.095423"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..386
                     /locus_tag="PKI94_08540"
     CDS             90..386
                     /locus_tag="PKI94_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26818.1"
                     /translation="MKLGAYENSISFQALRLRVPQGPNSKGSPDKILSQMGFQKVKEG
                     SQFEKDQFSLRIVPPMLGDRIPFDSPETHEILANPETENKIYKLLKKTFTIEKS"
     gene            complement(990..2447)
                     /locus_tag="PKI94_08545"
     CDS             complement(990..2447)
                     /locus_tag="PKI94_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNW26819.1"
                     /translation="MKRIILTLILILVSATSVFAAKIPDDVKGVIRKDFPRADFRFDG
                     LVTLPDGTLYLPLYPALVKKPDALVLKSTYPEGKTIADKPDIIILNNDFSFLKVITDK
                     KNRRTVINLPNPPVEVRTGLLPQDLLVPSGLIIPDNIKGIIGNLQIDTAKDAGLKVKT
                     DLVLQQRTTNEVFKKGQKNISLIPQLKNKVMYIATCYSKNVQVVEGEANSPAYALEQK
                     SIPIDIEITNDDKLLLVTTFGKTFVDVISLADERSIKQIDLTTQAEEIVIDRAANKAY
                     VSSGEDSSIYIIDLNTMTLKQKIKVKGMCQKLYLTDDGKKLFYFNKKNNDVWVIELDN
                     DFVIKNMGNFPNVSKITFAQNKVYITSRTKNRLAIVDYVTLALLKELEIQQKPIDMLV
                     YKNDLFILSAQNNVLQILDTINDEITGTINLNTQGFSTEIYRIKNSPVALVMDTKVGK
                     YSIIDLDKKSLIKTNLLNIPVSTVIVAEKVRKIGK"
     gene            complement(2458..>2895)
                     /locus_tag="PKI94_08550"
     CDS             complement(2458..>2895)
                     /locus_tag="PKI94_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNW26820.1"
                     /translation="KMRISQEGDAGKNGGPSGDLYVVIRVKPSDYLKREGINVFSEIE
                     ISPSQAVLGDEVVVKTLDGEKQISIPAGIQSASPIRIKSAGVPHLGKSSQRGEHIIIV
                     NVKTPTNLSEEEKQLYKKLYEINTKKKYQEGLFDKVKGWGVNK"
BASE COUNT          809 a          564 c          524 g          998 t
ORIGIN      
        1 aggataggat aggataggat agaaatttta atggcaaatt attttttgac gatttttatt
       61 atgaagtagt aaaaaataaa ggagaaaata tgaaattagg tgcttacgaa aattctattt
      121 catttcaagc tctaaggctt agagttcctc agggaccaaa ttctaaagga tcccctgaca
      181 agattttatc tcaaatgggg tttcaaaaag ttaaagaggg gagtcaattt gagaaagacc
      241 aattttcgct acgaatagtg cctccaatgc ttggtgacag gattcctttt gattctccgg
      301 aaactcatga aattttggct aacccagaga ccgaaaataa aatatacaag cttcttaaaa
      361 agacttttac aatagaaaaa tcataggtgg ttttgtagca agtatggtgc gcaatgcgca
      421 ccgaaaaata atttagcttt ggcgggaaat tttttaaaaa attccccacc tcttagtatt
      481 ttgaacttag gttggggttt gacttctttt caaggcgagg aacgagccgt agaaaatgtc
      541 cttacggttt gcattaatag tccattccag gttataattt tcaatagtat ctaccccaac
      601 agtattatgg tggggttccc aacccatcgt tttttggatt gccgcgtcat tcggcgttgc
      661 ctcactcctc gcaacgacgc aaaaatggtt aaccaaaagc caagaggcat ttaccccctc
      721 gcaatgacgt gaaatattag aagtttagca gcttggcagc ttagaagctt agcaatgcca
      781 aacttccaaa cctctaagct tccaagcttc aaaaactttt caacttatcg actcttcgac
      841 ttttcaacct ttcaggttgt aggatgcaga tttctgcacc tttttagtgt cctttatttt
      901 cggtgcgttt gtgcggacga aataatccaa aatgcgcttc cttttgcatt aacgcaccct
      961 acggctgttc acccttcacc gctcacagct cacttaccaa ttttcctgac tttttcggca
     1021 actattaccg tgcttacggg gatattcaaa aggtttgttt ttataagcga ttttttatca
     1081 aggtcaatga tagaatattt tccgactttt gtgtccatta caagcgcgac aggagaattt
     1141 tttatcctgt aaatttcagt cgaaaagcct tgagtgttaa ggttaattgt tcctgttatt
     1201 tcgtcgttta ttgtgtctaa aatctgaagc acattgtttt gcgcagaaag aataaacaaa
     1261 tcgtttttat aaaccaacat atcaataggt ttttgttgaa tttcaagctc ttttaaaagt
     1321 gcaagcgtaa cgtaatcaac tatcgcaagg cggttttttg tcctgcttgt aatataaacc
     1381 ttgttttgcg caaaagtaat ttttgaaaca ttagggaaat ttcccatatt tttaatcaca
     1441 aaatcattgt caagttcaat tacccaaaca tcattatttt tcttgttaaa atagaaaagt
     1501 tttttgccgt catctgtcag ataaagcttt tggcacatgc cttttacctt aattttttgt
     1561 tttaaagtca tggtgttaag atcaattata taaatgcttg aatcctcacc gcttgaaacg
     1621 taagccttgt ttgcagctct gtcgattaca atctcttctg cctgggtggt aaggtcaatt
     1681 tgttttatgc ttctttcgtc tgcaagcgaa atcacatcaa caaatgtttt cccgaaagtg
     1741 gttaccagaa gaagtttgtc atcgtttgtt atttcaatat cgataggaat agatttttgc
     1801 tcaagcgcat aagcgggcga atttgcctcg ccttctacca cctgaacgtt ttttgaataa
     1861 catgtggcta tatacatcac cttattttta agctgcggta taagcgaaat atttttctgc
     1921 ccctttttaa aaacctcgtt tgtagtgcgc tgttgaagca caaggtctgt tttaaccttt
     1981 agccccgcat cttttgcggt atctatttga aggtttccga taattccttt tatgttatcg
     2041 ggaattataa gtccgctggg aacaagcaaa tcctgaggca aaagccctgt tctgacttca
     2101 acgggcggat tgggaaggtt tatgaccgtt cttctgtttt ttttgtcggt aattactttt
     2161 aaaaaagaaa aatcgttgtt aagaattatg atatcaggct tatcggcgat ggtttttcct
     2221 tcaggatatg tacttttgag cactaaagca tcgggctttt tgactaaagc agggtacaaa
     2281 ggcagataaa gcgttccgtc tgggagggtt acaagcccgt caaacctgaa atcggctcgc
     2341 ggaaaatctt ttctgataac acctttaaca tcatcgggaa tttttgccgc aaatacactt
     2401 gttgcgctta ccagtattaa aattaaagtt aaaattattc ttttcacttc tcacccttca
     2461 cttatttaca ccccaacctt ttactttatc aaaaagtcct tcttgatatt tctttttagt
     2521 attgatttca tataattttt tgtaaagctg tttttcttct tcagaaagat ttgttggagt
     2581 tttcacattt acaattatta tgtgttcgcc tctttgagaa gatttaccca agtgcggtac
     2641 gccggcggat ttaattctta tcggacttgc gctttgtatt cctgcaggaa ttgaaatttg
     2701 tttttcaccg tcgagcgttt ttacaacgac ctcatcgcct aaaaccgcct gtgatggtga
     2761 aatttcaatc tcggagaata catttatccc ttctcttttt aaataatctg aaggttttac
     2821 ccttattaca acgtacaaat cccctgaagg gccgccgttt ttacccgcat ctccttcttg
     2881 cgaaatcctc atttt
//
LOCUS       DAOQAU010000087         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_51826_length_2744_cov_8.364076, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000087 DAOQAU010000000
VERSION     DAOQAU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51826_length_2744_cov_8.364076"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..550)
                     /locus_tag="PKI94_08555"
     CDS             complement(173..550)
                     /locus_tag="PKI94_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26821.1"
                     /translation="MTNLKISFNGELTIRTWDDAENKMQIKYVNTKKCDDKKLIQAAK
                     SILEPKGKMLREVLDSDKTKELDSLLEEILEQKMPNENFIRTMLNDGKNFFYMLTNPV
                     SYLYGSAMPSPLMQGGIGIDFVA"
     gene            complement(643..1512)
                     /locus_tag="PKI94_08560"
     CDS             complement(643..1512)
                     /locus_tag="PKI94_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HNW26822.1"
                     /translation="MKRLAIFAHWDKDNLIEDYVLYYLQNLKNIAQKIIFVSDCELTD
                     EQKEKVSNIVDIVICEKHGEYDFGSYKRGFFAAVKDNMIEDFDELIFANDSCYAPMFP
                     FEKMFEEMEGKNIDFWSASRNYFGIGFENGKPVRIVEPHLQSYFLVFKKQVFTCEDFI
                     EFMKNIKKEPNKINVIINYELGLNKNLKEAGFVGRAYAEEYPQISNPTLCLWDKLVKN
                     GHPFIKTSVLRCNNIDIVYPFKWKNLLKTHTNYPVKLIEEDLKRNKSKIEPKFFWKIL
                     KQRIKQLKNKIRN"
     gene            complement(1534..2415)
                     /locus_tag="PKI94_08565"
     CDS             complement(1534..2415)
                     /locus_tag="PKI94_08565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW26823.1"
                     /translation="MFNLNFKISIITASYNYENFIKETVESVIAQTYSEWELIIVDDG
                     STDNSVDVIKDYCKKDDRIKFFQHENGINKGLKETLLLGIEKASGKWIAFLESDDIFE
                     PTYLEEKIKAINLTKDVGLVFNDVKMFGDKEQIETYGDYLNRREKMLSSDRLKYTDIF
                     KDNPIQTFSCVIVKKELLTNCNFNSPVSQNLDWLLWAQVINKTKFVYIQKPLTRWRIH
                     SRSYIKSTNRENRGEWLAKLLMALDEKKHCPLWYKFFELINKLSVEKLLRPQVNFISN
                     LIVNLFLGKKSAELVKF"
     gene            complement(2430..>2744)
                     /locus_tag="PKI94_08570"
     CDS             complement(2430..>2744)
                     /locus_tag="PKI94_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26824.1"
                     /translation="KLSIAEAKKYSAKKVDVEMLLEEISEKFDRQQEKIDELESKIEK
                     LFKTRPSNMNEFDIKAIVEEVLAQVGGGKGDAKLSKKVDSIEKQITQLSKNIEKLTSY
                     VD"
BASE COUNT          793 a          468 c          404 g         1079 t
ORIGIN      
        1 aaaagggggg gtaatttttt attcgttttg ataagttcag gatgacgcaa gaggcgggct
       61 attttttctt aataaaagta caatcagtgt cacataaaaa ggtaactcgg gttgtaatat
      121 taaaattata aaaaaaattt ttttagttgt tttaaaattc cccaaaaaga tattaagcta
      181 caaaatcaat tcctattccg ccttgcatca gaggacttgg catggcagag ccataaagat
      241 aagaaaccgg gtttgttaac atgtaaaaaa agtttttgcc atcgtttagc atggttctta
      301 taaaattttc gttgggcatt ttttgttcaa gaatttcttc taataaagaa tccagctctt
      361 ttgttttatc tgagtctaaa acttctctta gcattttacc tttaggttct aatatcgatt
      421 tagcggcttg tattaatttt ttgtcatcac atttttttgt gttgacgtat tttatttgca
      481 ttttgttttc ggcatcgtcc caggttctta tggttagttc gccgttgaaa gaaattttaa
      541 ggtttgtcat aatataatcc ttgatttata acagtagtat tatagaatag aaaattaaaa
      601 aagtatcagt ttataaaatt ttaatatagg ggtaaatgta tttcagtttc ttatcttatt
      661 ttttagctgt tttattctct gctttaaaat tttccaaaaa aattttggct ctatttttga
      721 tttatttctt ttcaaatctt cttcaattaa ttttacagga tagtttgtgt gggtttttaa
      781 aaggtttttc catttgaaag gatagactat atcaatgtta ttgcacctta aaacgcttgt
      841 ttttataaaa ggatggccgt ttttaactaa tttatcccat aaacataaag tcgggttgct
      901 tatttgcgga tattcttctg catacgccct ccccacaaag cccgcttctt tcaagttttt
      961 gtttaatccc aattcataat ttattatcac atttatttta tttggctctt ttttgatgtt
     1021 tttcataaac tcaatgaaat cttcacaggt gaaaacctgt tttttaaaaa caagaaaata
     1081 gctttgaagg tgaggttcaa caattcttac cggtttcccg ttttcaaatc ctatcccgaa
     1141 gtaatttctg cttgcgctcc aaaaatcaat gttttttcct tccatttctt caaacatttt
     1201 ttcaaacgga aacatcggag cataacagct gtcgtttgca aagattaact cgtcaaaatc
     1261 ttcaatcata ttatctttaa ctgccgcaaa aaatcctctt ttgtatgaac cgaaatcgta
     1321 ttctccgtgt ttttcgcaaa tcactatatc gacaatattg cttacttttt ctttttgttc
     1381 atctgtaagt tcgcagtctg atacaaaaat tattttctga gcgatatttt tcaaattttg
     1441 cagataatat aaaacgtaat cttcaattaa attatcttta tcccagtgtg caaaaattgc
     1501 caatcttttc attctactcc ctttcggata aatttaaaac ttaaccaact ccgctgattt
     1561 tttgcctaaa aacagattta caatcaaatt acttataaaa ttaacctgcg gacgcaaaag
     1621 tttttctact gaaagtttgt ttataagttc aaaaaactta taccacaaag ggcagtgttt
     1681 tttttcatct aacgccataa gtaattttgc aagccattca cccctgtttt ccctatttgt
     1741 gcttttgatg taacttcttg aatggattct ccagcgggtt aggggttttt gtatgtaaac
     1801 aaattttgtc ttattgataa cttgtgccca caaaagccaa tccaaatttt gcgagacggg
     1861 tgaattgaaa ttacaatttg tcagcaattc ttttttcact atgacacacg aaaaagtctg
     1921 aatcggatta tctttaaaaa tgtcagtgta tttaagtcta tctgatgaaa gcattttttc
     1981 cctgcgattc agataatcgc cgtaagtttc aatctgctct ttgtcgccga acatttttac
     2041 atcgttaaac actaatccca catcttttgt aagatttatt gcctttattt tttcttccag
     2101 ataagtcggc tcaaaaatat catcgctttc cagaaaagca atccatttgc ccgaagcctt
     2161 ttcaattcca agaagtaaag tttcttttaa gcctttgttg atgccgtttt cgtgttgaaa
     2221 aaattttatt ctgtcgtctt ttttgcagta atcttttatg acatcaacgg aattatccgt
     2281 cgagccgtca tcgacaataa tcagttccca ttcggagtat gtttgagcta taacactttc
     2341 aacggtttct tttataaaat tttcgtaatt gtaacttgct gttataatcg atattttaaa
     2401 attaagatta aacattgttt tatgacctat taatccacat aagaagttaa tttttcaata
     2461 tttttactaa gttgggttat ttgtttttcg attgaatcaa cttttttgga aagttttgca
     2521 tcgccttttc caccgccgac ctgtgcaaga acctcttcta cgattgcttt aatatcaaat
     2581 tcgttcatat ttgaaggacg agttttgaaa agtttttcta ttttggattc aagttcgtcg
     2641 attttttcct gctgacggtc aaatttttcg gatatttctt ctaagagcat ttccacatcg
     2701 acttttttgg ctgaatattt tttggcttct gcgattgaaa gttt
//
LOCUS       DAOQAU010000088         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_53530_length_2685_cov_10.242966, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000088 DAOQAU010000000
VERSION     DAOQAU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53530_length_2685_cov_10.242966"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2365)
                     /locus_tag="PKI94_08575"
     CDS             complement(<1..2365)
                     /locus_tag="PKI94_08575"
                     /inference="COORDINATES: protein motif:HMM:NF020379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT4734/BF3469 family protein"
                     /protein_id="HNW26825.1"
                     /translation="MLQTKISIFKNIKDSQNPYDRPVSDFLNRIKEGSNATNLVLKYR
                     ETKDDKYKQALPCCTFSGTFTRRRDANIVDFSQFACLDFDKFEDEAEVLKLKNKLSKE
                     PSLFCAFISPSGKGLKAIFRVENNPERYESLYRALCTKYDDEHIDKGTKDISRCCYES
                     YDPDIYINANAKEWNVYEEEEYTHLGVERYNVVVPLKSESRIIDNIQKWFDKKYSMAL
                     GDRNNNVFKFACALNAYGINEITTLNYLQKYAQKGFTSKEIEQAIKSAYKRYKGEFNT
                     RAFEDNETRIQIQKAVSSGKTKQEIKKDFEKKNNPIVGDDALFEEILETVKTAESVDV
                     FWNINEKGKIILDPFLFEQYLKSNNFMKFYPESSSDTFIFVKKNKSLIEETNRDKIKD
                     FVLNDLKTRKDIGFAPYNYMAMNTKYFSNDFLNMLESVEIEIKKDTADQCFLYYKNCV
                     VEVGKDYVKEIDYLNIDKYVWQDSVIERDFKIFDHHKCVFRSFIWYISGQNKDAYNSF
                     KSAIGYLLHSYKTQADNKAIILNDEIITDEPNGRSGKGVFFNGIKQLKKLVALNGKKI
                     DLNSQFAFQTVKADCQVLVFDDVKRNFPFEDLFSIITEGLEIEYKGQGAIKLPVTKSP
                     KIVITTNYTIKGEGGSFDARKFELELSSYFNSKFSPKDKFGHMFFDEWDSDEWSRFDN
                     FMIQCIQYYLVNGLQNQNHKNLEERKYISETSQDFHEWTKEGHLEEGKRYYNSNSFDL
                     FIEEYPDYRNKLTIKTFKKWIKKYCEFNKLKYSDGTANSLRYFYISKD"
BASE COUNT          827 a          469 c          342 g         1047 t
ORIGIN      
        1 tgtctttact gatgtaaaaa tacctcaaag agttagctgt tccatctgaa tattttaact
       61 tattaaattc acaatatttt tttatccatt ttttgaaggt ttttatcgtc aatttattcc
      121 gatagtctgg atattcctct ataaataaat caaaagaatt agaattgtaa taccttttgc
      181 cttcttctaa atgtccctct tttgtccatt cgtggaaatc ttggcttgtt tcgcttatgt
      241 attttctttc ctctaaattt ttatgatttt ggttttgtaa cccattaaca agataatatt
      301 gaatacattg tatcataaaa ttgtcgaacc tactccactc gtctgaatcc cactcatcaa
      361 aaaacatatg accaaacttg tcttttgggc tgaattttga attaaaataa ctacttaact
      421 ccaattcaaa ttttcttgca tcaaatgagc caccctcgcc ttttattgtg taatttgttg
      481 taatgactat ttttggagat tttgttacag gtaactttat tgctccctgt cccttgtact
      541 ctatttctaa accttctgtt attattgaaa ataagtcttc aaaaggaaaa ttgcgtttta
      601 cgtcatcaaa aacaagcact tggcaatcgg ctttcacagt ttgaaaagca aattgagagt
      661 ttaaatctat tttttttccg ttcaaggcga ccaatttttt taattgtttt atgccattaa
      721 aaaatacccc tttcccactc ctaccatttg gttcatcagt tattatctcg tcattaagga
      781 ttatggcttt gttatctgct tgtgtcttat aagagtgcaa cagataccca atagcagact
      841 taaagctatt atacgcatct ttgttttgcc ctgaaatgta ccaaataaaa gagcgaaaaa
      901 cacatttatg atggtcgaaa attttaaaat cacgctctat taccgaatct tgccaaacgt
      961 atttatctat gtttagataa tcaatttctt ttacatagtc tttaccgacc tcaacaacgc
     1021 aatttttata atacaaaaaa cattggtcgg cagtatcttt ctttatttca atttcaaccg
     1081 actccaacat atttagaaaa tcatttgaaa aatatttcgt gttcatagcc atataattat
     1141 acggagcaaa cccaatatct tttcttgttt ttaagtcgtt tagtacaaaa tcttttattt
     1201 tatccctgtt ggtctcttca atcaaacttt tatttttctt tacgaaaatg aaagtatctg
     1261 aactgctctc agggtaaaat ttcataaaat tatttgattt taaatattgc tcaaaaagaa
     1321 aagggtcaag tattatttta cctttttcgt ttatgttcca aaacacgtca accgactcag
     1381 ctgttttaac tgtttctaaa atttcttcaa aaagtgcatc atcgccaaca ataggattgt
     1441 ttttcttttc aaagtctttt tttatttctt gttttgtttt acctgaactg acggcttttt
     1501 gtatttgtat tctcgtttcg ttgtcttcaa aagctctggt gttgaactct cctttgtatc
     1561 ttttgtatgc tgattttatt gcttgttcta tttcttttga agtaaaacct ttttgagcat
     1621 atttttgtag gtaatttaaa gtcgttattt cgtttattcc gtaagcattc aaagcacaag
     1681 caaacttaaa aacattattg tttctatctc ctaatgccat tgagtacttc ttgtcaaacc
     1741 atttttgaat attgtcaata atacgacttt cagattttaa aggcacaaca acattatatc
     1801 gctcaactcc caaatgtgta tattcttctt cttcgtaaac attccactct tttgcatttg
     1861 cgtttatata aatatcaggg tcgtaacttt cataacaaca tcttgaaatg tcttttgtac
     1921 ctttgtcaat atgttcatcg tcatattttg tgcataatgc tctgtataat gattcgtatc
     1981 gctctggatt gttttctacc ctaaatatag ccttcaatcc ttttccgctt ggagaaataa
     2041 aagcacaaaa caaagaaggt tcttttgaaa gtttattttt aagcttcaaa acctctgcct
     2101 cgtcttcaaa tttatcgaag tctaaacagg caaactgact gaaatcaaca atgttggcat
     2161 ctctccttct tgtaaatgta cctgaaaatg tacaacaagg taaagcctgt ttgtatttgt
     2221 catctttagt ttctctatat tttaaaacta agtttgtagc atttgacccc tcttttatac
     2281 gatttaaaaa atcagacaca ggtctatcat aaggattctg agaatctttt atgtttttga
     2341 aaattgatat ttttgtttgt agcataaatt gtcgaatttt atttaagttt gatgtttttt
     2401 tgtataggtt tttagtaata tatactttat cgtactttat cgtactgcta aagtactgac
     2461 taatttattg atatacaact acttaaccta taaaagtacg aaagtacgat aaaaaatgga
     2521 aaattttcaa aataaaaaaa tacaaaaaaa tattctctat aaagcaataa agggaaatcg
     2581 tactttcgta cttaacgctt gtattttttt aaaaagactc ccgcaaatca atgcaatact
     2641 caaaagcatc tttataactt tcaaactcga aaatagaaaa attca
//
LOCUS       DAOQAU010000089         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_77456_length_2113_cov_7.113217, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000089 DAOQAU010000000
VERSION     DAOQAU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77456_length_2113_cov_7.113217"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..342)
                     /locus_tag="PKI94_08580"
     CDS             complement(157..342)
                     /locus_tag="PKI94_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26826.1"
                     /translation="MNTGYQIESVEITNDSLVDIKANDVQTHWIAEFIKMERKYIIFK
                     PDGSLTPLQTYDIKDNE"
     gene            complement(671..1939)
                     /locus_tag="PKI94_08585"
     CDS             complement(671..1939)
                     /locus_tag="PKI94_08585"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNW26827.1"
                     /translation="MGKIKETKGTGKEEQTKFVGQPIFKQVIDLMERIDLKGIIRKHN
                     SDYYYKEFKSKLHLITMLFGILSRCDSMTEICEGLRALGGKLNHLGLEKAPAKSTASD
                     GLRNRDSSFFETVYFSLVNHYKSFLSDSRTYGLTFKEVLLIDSTTIRLFSDILKGVGR
                     NPKGDGKKKGGLKVHMLIDAVQSVGRFIKITAAKVHDKNFLKSLELISHSMVVFDRAY
                     NYYHQFALWTNKEVYFVTRLKKNAVYLVIEVKRNHYKKKGQAKVLRDEIIEIEYHPED
                     QNGKPLTNVKKIVQLRKVCYQDERNRYYEFLTNNFEITAEEVAFLYKKRWGIELLFKK
                     MKQNFQLHYFYGENVNAIYTQVWCTLIAQLLLTVVQKIANAKKAFSVVASLVRIHLIS
                     LLDVYELLRSTKREYACRYKPPPTGQLCIF"
BASE COUNT          589 a          398 c          338 g          788 t
ORIGIN      
        1 atgttttctc tttcatattt tttgtgtttt ggtttttttg cccttgccta taacgaaccg
       61 gtgctgctgc atggcggggc atctttgatg tcacgcttga atgtcgcaag acagtcaacc
      121 gctggcagca gcacaaagtt atacacaggt tgttctttat tcattgtcct ttatatcata
      181 agtctgcaat ggagtcagac tgccgtcagg tttaaaaata atatattttc tttccatttt
      241 tataaattct gctatccaat gggtttgaac gtcatttgct tttatgtcaa caaggctgtc
      301 atttgtgatt tcaacagatt cgatttgata tccagtattc attttgtcgt taaaaaaaga
      361 aatatttaag tcgtcaaaac tcttaaattg tagttcgata ttcctaatta tttcggatat
      421 tatatttaag tctttacaag tattcttgtc agtaacgaca aatactcttt ggtttgttga
      481 atcgatttta taaatctcat aagtctttgt ctgagcaaat atagccaaag gtgaaaaagt
      541 caatagaaag ataattacat ataaaatcac tattgtccga ctaaatttga caataaggca
      601 aatattgtct taatcatttg aaaatcagtc attgtattat tattttctaa aagcacccct
      661 cggtatttca tcaaaatatg catagttggc ctgttggcgg tggtttgtat ctacacgcat
      721 attctctttt tgtgctacgc aaaagttcat atacatcaag tagacttatt aaatgaattc
      781 tcacaagtga cgctaccaca gagaaagcct tttttgcatt agctattttt tgaaccactg
      841 tcaaaagtag ttgtgcaatt agtgtacacc aaacctgcgt atatattgcg tttacatttt
      901 ccccatagaa atagtgaagt tggaaatttt gtttcatttt tttgaacagt agctcaattc
      961 cccaacgctt cttgtataga aaagcaactt cttcagccgt aatctcaaaa ttatttgtta
     1021 gaaattcata ataccgattg cgttcatctt ggtagcaaac ttttcttagc tgtactattt
     1081 tctttacatt ggtcaatggc ttaccgtttt gatcttcggg gtgatattca atttctatga
     1141 tctcatccct gagaactttt gcttgccctt tctttttgta atggtttctt tttacttcta
     1201 taacaaggta tactgcattc ttttttaatc gagtaacaaa atacacttcc ttattagtcc
     1261 aaagcgcgaa ttggtgataa tagttataag ccctgtcaaa aacaaccata ctgtgtgata
     1321 taagttcaag acttttcaag aaatttttgt catgtacttt tgcagcagta attttgataa
     1381 atcttcctac cgattgaacg gcatcaataa gcatatgaac ttttaatcca ccttttttct
     1441 tcccgtcacc tttgggattg cggcctactc ctttcaatat atcgctgaat aaccggattg
     1501 ttgttgaatc tatcagtaga acctctttga atgtcaatcc ataggttcgg ctgtccgaca
     1561 aaaaactttt ataatgatta accaaagaaa aatacactgt ctcaaaaaaa ctgctatccc
     1621 tgtttcgtaa accatcactg gcggttgatt tggctggcgc tttttctaag ccaagatggt
     1681 ttaatttgcc gcctaaagcc cttagccctt cgcaaatctc agtcatcgaa tcacagcggc
     1741 ttaatattcc gaacaacatt gttatcaaat gtagttttga cttgaactcc ttataataat
     1801 aatcggaatt gtgcttcctt attatccctt ttaaatcaat cctttccatt aaatcaatta
     1861 cttgtttaaa aatcggctgt ccgacaaatt ttgtttgttc ttctttgcca gttcctttgg
     1921 tttctttaat tttacccatg ttgtttatgt tttgccaaaa acaaatttac aacacggggg
     1981 gaaaccttcg ggtagtaccc cttttttttt agtcgtttag tagtgatata aaatcttttt
     2041 tttcatttta acttgtgtat aacgttccgg ggctttgcag cgggcgggtt tttaactgtc
     2101 ctgctgtgtc aac
//
LOCUS       DAOQAU010000090         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_90022_length_1916_cov_8.495433, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000090 DAOQAU010000000
VERSION     DAOQAU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90022_length_1916_cov_8.495433"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKI94_08590"
     CDS             <1..218
                     /locus_tag="PKI94_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW26828.1"
                     /translation="EHINPEYNVIDIEAFALAKVAEQEKIDFLCLKYISDGADGNAAN
                     DWTQEVKKASIALKRELDSRSLTKPTI"
     gene            complement(198..1544)
                     /locus_tag="PKI94_08595"
     CDS             complement(198..1544)
                     /locus_tag="PKI94_08595"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNW26829.1"
                     /translation="MLGKNPEKKPELFRPMLVDFIDHEHELVLLSEKIDWNYFEKEFS
                     PLYSKVGNPSHPIRFMVGCLLLKHLYNLGDETLEKAWIMNPYMQHFCGRVFFEHEFPC
                     DPSNFVHFRKRIGEKGIEKIFAYSVRMHDAKTNTSNFVLSDTTVQENNTSFPTDAKLC
                     KKVIDYCNKIAGNEGIKQRQRYTKVSKQMVRNTYNGKHPKRAKAARKSQRQLKTIAMR
                     LIRELQRNFNAEQQEFYKDLMTLYTKVVTQKRNDADKIYSIHKPFTRCIAKGKAHSQY
                     EFGNKVGLITTANKGKKIILGIKAFLQTPYDGHTIEPLLEQMETGGQKLPKELVYDRG
                     GRGKSEIKGVKISIPSTPRKKDTAYQKQTKRKKFRTRAAIEPIIGHLKTDFRLAKNYF
                     MGETGPQINALLAATAWNMKKMMELLKQKIIFLFYKIQIMLFSNPVFKNKLNSGFC"
BASE COUNT          564 a          344 c          347 g          661 t
ORIGIN      
        1 tggagcatat caatcctgaa tataatgtca tcgatataga agcttttgcg ttggcgaaag
       61 tcgctgagca ggaaaaaatc gattttctat gtttgaaata tatttctgat ggagcagacg
      121 gaaatgctgc taatgattgg acacaagaag tcaaaaaagc ttcaatcgca ttaaaaagag
      181 aactagatag tcgttcctta acaaaaccca ctatttaatt tatttttaaa aacaggatta
      241 gaaaacagca taatttgtat cttataaaat aagaaaataa ttttctgttt cagtagttcc
      301 atcattttct tcatgttcca agcggttgca gctagtaatg cattgatttg tggtcccgtt
      361 tctcccatga agtaattttt tgccagccta aaatcggttt ttaaatgtcc gatgataggt
      421 tctattgccg ctctggttct aaattttttg cgctttgtct gcttttgata agcagtgtct
      481 ttttttcttg gagtgcttgg gatggagatt ttcacgccct ttatttctga ttttcctctg
      541 ccacctctat cgtaaacgag ttcttttggg agcttttgac caccggtttc catctgttcc
      601 aaaagtggtt ctatggtgtg accatcgtaa ggagtttgca aaaatgcttt aatcccgaga
      661 atgattttct tgcctttgtt ggcggtggtt atcaaaccta ccttattccc aaattcatac
      721 tggctatgcg cttttccttt ggcaatacat cgggtaaaag gcttgtgaat gctgtaaatt
      781 ttatcggcat cgtttctttt ttgtgtgaca accttggtgt acaatgtcat taaatcttta
      841 taaaattctt gctgttctgc attaaaattc cgttgcaatt cacgaatcag tctcatggcg
      901 atggttttga gctgtctttg agatttcctt gccgcttttg cccgcttggg atgttttccg
      961 ttgtaggtgt tgcgcaccat ttgtttgctg acttttgtgt agcgttgtct ttgttttatg
     1021 ccttcatttc cggctatttt gttgcaataa tcaatcactt ttttgcacaa ttttgcatcg
     1081 gtaggaaaag aggtattatt ctcctgaacg gtagtatcgg acaaaacaaa atttgaggtg
     1141 ttcgtcttgg catcgtgcat tcttacgctg taggcaaaga ttttttcgat acctttttcg
     1201 ccaattcttt ttcggaaatg aacaaaatta ctcgggtcac aaggaaattc gtgttcaaag
     1261 aaaaccctgc cacaaaaatg ctgcatataa ggattcatga tccaggcttt ttccaacgtc
     1321 tcatcgccca aattatacaa atgtttcagt agcaaacaac ccaccataaa ccgaatcgga
     1381 tggctcggat tgcccacttt ggaatacaag ggcgaaaatt ctttctcaaa ataattccaa
     1441 tctatttttt ctgaaagtag aacaagttca tgctcgtggt caataaaatc caccaacatt
     1501 gggcggaata attctggctt cttttctgga tttttcccca acatatttgc aaggttttaa
     1561 agctctaaga tacaaattct tgcaataaaa aacaagctat tttaaaccca aaatactaat
     1621 aatcagtaaa ttataggtgg tttaaggaga gactacttaa accttacaaa ctggctgaac
     1681 aaatgattgg ggaaaattat atcaatactt cgctaaaaag tcatataaaa tattgatttt
     1741 cagtaaaata agctaaaaat aattggttaa aaaattggat aactcgttga taatcagtat
     1801 atttatgttt gaaaaagaac atacaaaacc ccgagttatc cagtgttaaa agtattgttt
     1861 tttctgcatt tgagcaaatg gaaaaacaat tagattgtag aatcccaaaa agtttc
//
LOCUS       DAOQAU010000091         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_90028_length_1916_cov_6.442235, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000091 DAOQAU010000000
VERSION     DAOQAU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90028_length_1916_cov_6.442235"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>1916
                     /locus_tag="PKI94_08600"
     CDS             24..>1916
                     /locus_tag="PKI94_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HNW26830.1"
                     /translation="MFFIDTKKPVTKPSNYNDFCMSFFQNNSNNCRHCIASNLKCIKQ
                     ALKADKTIICTCHNGLSIFAIPIKLQNEPLGAILGGQVFTEKPDSKKIKQIINKLNVN
                     EKKYTQVMKEVPVIAKEKLVEITQNLSAFTNFISKMAQKNYEITKQSKRNKILRNIVE
                     ILRSTLEPDEIKKFFLETASKYFEPDRCMFVDYDSETKKFLPFRLELLKSNDIPSFVG
                     IDTETVFPEFCQKLKAKQRNIIVKDLEKTLSRKKLLSYEAVKSLEGSRVKSDYGLVVE
                     YQGEIMGLLILHYIKEKRVLNSEEFSFLKVLRDNVGTALHLAELYEKSVRQTQREKLI
                     SNVLAKAISTFDINNIKQIVNEIGTMMKADRCYFVEADMENLKGKSIDYEGEYLSSSD
                     IKSIIGYDFPSEDVKLFIDLFTQAGDLVVFDYEKMREENEEKYAGISRYSKRFEIKSG
                     IGIPIISEGKLKALLAIEYAKEKVLPPDEELDFLRILGNQVGMVFNQIQLYKNTEKLA
                     QREILIREITETIRQSLDINKTKKIISEIMGKTLKADRCFIFEYNEANDKILIVSDEY
                     LSSDKILPYAGKDLNEHIPSLAAELKKGKRYIVNEKGATLGGEKVNLEDGHHEDVLAA
                     IKEYKIN"
BASE COUNT          806 a          236 c          342 g          532 t
ORIGIN      
        1 gaaatggtgg gaagcttgaa ataatttttt tcattgacac taaaaagcct gtaacaaagc
       61 cgagtaatta caatgatttt tgcatgagtt tttttcaaaa taattctaat aattgtcgcc
      121 actgtatagc gtcaaattta aaatgcataa aacaggcttt aaaagctgat aaaacaataa
      181 tttgtacctg tcataacgga ttatcgattt ttgctatacc tataaaatta caaaatgagc
      241 cattaggtgc aatattggga gggcaagtct ttacggaaaa acccgattca aaaaaaatta
      301 aacaaataat aaataaatta aatgtaaacg aaaaaaaata cacgcaagta atgaaagaag
      361 tacctgttat agcaaaagaa aaactggtag aaataacaca aaacttatct gcttttacaa
      421 attttatttc aaaaatggca caaaaaaatt atgaaattac aaaacaaagc aaaagaaata
      481 aaattttaag aaatatagtt gaaattctca gaagcacgct tgaacctgat gaaattaaaa
      541 agtttttttt ggaaacagca agtaaatatt ttgagcctga cagatgcatg tttgttgatt
      601 atgatagtga aacaaaaaaa tttcttcctt ttcgtcttga gttactaaaa tcaaacgata
      661 taccaagttt tgtaggtatt gatacagaaa cagtctttcc tgagttttgc caaaagctta
      721 aagcaaaaca gcgaaatatt atagtaaaag acttagaaaa aacactttcc agaaaaaagc
      781 ttttaagtta tgaagcagtt aaaagcctgg aaggaagcag agtaaaatca gattatggtc
      841 ttgtggttga atatcaaggc gaaattatgg ggctgcttat acttcattat ataaaggaaa
      901 aaagagtatt aaatagtgaa gaatttagtt ttctgaaagt tctaagagat aatgtcggaa
      961 ccgcacttca tcttgcagaa ctttatgaaa aaagtgttcg ccaaacacaa agagaaaagc
     1021 ttataagtaa tgttttagct aaggcaataa gcacttttga cataaataat attaaacaaa
     1081 tagtaaacga aataggaacg atgatgaaag cagacagatg ctattttgtt gaagcagaca
     1141 tggaaaatct aaagggaaaa tcaattgatt atgaaggaga atacttatcc tcttcggaca
     1201 taaaaagtat catagggtat gactttccaa gtgaagatgt taagttgttt attgatttgt
     1261 ttacgcaagc aggagacttg gttgtttttg attatgaaaa aatgcgtgaa gaaaatgaag
     1321 aaaaatatgc ggggataagc agatacagca aacgatttga aattaaaagc ggaataggca
     1381 tacctattat ttcagaagga aagttaaaag cattattggc tatagaatat gcaaaagaaa
     1441 aagtacttcc gcctgatgaa gaacttgatt ttttaagaat tttgggaaac caagtaggga
     1501 tggtttttaa tcaaattcaa ttatataaaa atacagaaaa acttgctcaa agagaaattt
     1561 taatcagaga aattacagaa acaataaggc agtctttaga tatcaacaaa acaaaaaaaa
     1621 taatatctga aattatggga aaaactttga aagcagacag atgctttata tttgaatata
     1681 acgaagcaaa cgacaaaatc ttaatagtaa gcgatgaata cttgtcttct gataaaatcc
     1741 taccatatgc gggtaaggat ttgaacgaac atatacctag tcttgcagct gagttaaaaa
     1801 agggtaaaag atatatagta aacgaaaaag gagcaacact cggtggagag aaagtaaatt
     1861 tagaagacgg acaccatgaa gatgttttag cggcaataaa agaatataaa attaat
//
LOCUS       DAOQAU010000092         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Gastranaerophilaceae bacterium isolate
            AMR_MDS_1254 NODE_107428_length_1713_cov_6.660434, whole genome
            shotgun sequence.
ACCESSION   DAOQAU010000092 DAOQAU010000000
VERSION     DAOQAU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854459
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Gastranaerophilaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Gastranaerophilaceae bacterium
            Bacteria; Bacillati; Candidatus Melainabacteria; Candidatus
            Gastranaerophilales; Candidatus Gastranaerophilaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,721
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,678
            CDSs (with protein)               :: 1,678
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Gastranaerophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107428_length_1713_cov_6.660434"
                     /isolate="AMR_MDS_1254"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022870"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1645
                     /locus_tag="PKI94_08605"
     CDS             155..1645
                     /locus_tag="PKI94_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNW26831.1"
                     /translation="MYKIRQIQYSSKSVSVQVYTILNRKRVILRHIGTARTEKEKADL
                     ITLANDFIEKLSKQLNLFENKESGNILHVSQTEFIGVYYGFLYELLSKLVIKIGFDKI
                     RNVLLTDLSIIRIFEPASKLRSIELLEDYFGIKHRRQGYYESAPKWLDLKGKIEKIAI
                     AFAKKNYSFNYDLLFYDVTTLYFETFEEDEFRKDGFSKDNKSQQPQVLVALLVTKEGF
                     PIAYEVFPGNTFEGHTILPVVQNFILKNQVKEFTVVADAAMISAGNIRQLTESNINYI
                     VGARLGNISNELFLEIDRNIIRKDGKTIRIKTELGYLICGYSSLRYRKDKHEMEKQIE
                     KAKQVVENPSKSKKLKFTKAKGQNIVLNQELIARTQKLLGIKGYYTNLEEATASNQLI
                     MERYHELYRIEQAFRISKHDLQTRPIFHFKEEPIKLHILICFVALVISKHIELQTDTS
                     IKKIIYECKKVTDARLLNKITNREIRIRVKLSEKIIGIMMRLSLLT"
BASE COUNT          602 a          297 c          329 g          485 t
ORIGIN      
        1 tgtgtggtta gcttataaaa ggtctgacag gttgtagaaa tctgtcagat cttttcctga
       61 cttgtccgcc ttctgttatg gtaaaacata atgcattggg aaagagttaa ttaattttaa
      121 aaaaattgta atttgctgac ctaaatggtc aaaaatgtat aaaatcagac aaattcagta
      181 ttcttcaaaa tctgtttccg ttcaagtcta tacgatatta aatagaaaaa gggtaattct
      241 aagacatatt ggtactgcaa ggaccgaaaa agagaaggca gacttaataa ccctagctaa
      301 tgattttata gaaaagctct ccaagcaatt aaatcttttt gaaaacaaag aatctggcaa
      361 catcctccat gtttcccaaa cggagtttat cggagtgtat tacggttttt tgtatgaact
      421 tctctctaaa cttgttataa aaatcggatt tgataagatc cgtaatgtgt tattaacgga
      481 tttaagtatc attaggattt ttgaacctgc ctcaaaactc cgttccatag agcttttaga
      541 agactacttt ggcataaagc accgcaggca gggttattat gaatctgccc ctaaatggct
      601 cgatttgaag ggaaagattg agaaaattgc aatcgctttt gcaaagaaaa actattcgtt
      661 caactacgat ctgttgtttt acgatgtaac gaccctctac tttgagactt tcgaggaaga
      721 tgagttcagg aaggacggct tttctaagga caacaaatcc caacagcctc aggttctggt
      781 agccttattg gtaaccaaag aaggctttcc catagcctac gaagtgtttc caggcaatac
      841 ttttgaaggg cacaccattt tgcctgtggt gcaaaatttt attcttaaaa accaagtaaa
      901 agaatttacg gtagtggccg atgcagcgat gataagtgcc ggaaacatta gacaactaac
      961 cgaaagcaat atcaattaca ttgttggtgc aaggctggga aatatatcta acgagctctt
     1021 tttagaaatc gacagaaaca ttattcgtaa agacggtaaa actatcagga ttaagacaga
     1081 actcggctac ttgatttgcg gctattcttc tcttcggtac cgaaaagata aacacgaaat
     1141 ggaaaaacag attgaaaagg cgaaacaggt tgttgaaaat ccttcaaaaa gtaaaaaact
     1201 aaagtttaca aaagccaagg ggcagaacat agtactcaat caagaactca tagcccgaac
     1261 ccaaaaactg cttggcatca agggctatta tacaaattta gaagaagcta cagcaagtaa
     1321 ccaactcatt atggagcgct atcatgaact ttacagaatt gagcaggctt ttagaatatc
     1381 caaacacgat ttgcaaacaa ggccgatttt ccatttcaag gaagagccaa taaagctgca
     1441 tattcttatt tgttttgtag cccttgtaat ctcaaagcat atcgagcttc aaacagacac
     1501 aagtattaaa aaaattattt atgagtgcaa aaaggttaca gatgcacgat tactcaataa
     1561 gatcacgaat agggaaatta gaattagagt aaagttgtca gaaaaaatca ttggaatcat
     1621 gatgcgttta agtctgctga cctaatttgg acaagtcagg aatgaaaaat cgatgagctc
     1681 gttaattctt cgaagaaggt tatcctttgg tac
//
